LOCUS       DAVKMU010000001       118779 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKMU010000001 DAVKMU010000000
VERSION     DAVKMU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 118779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.001"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            491..1009
                     /locus_tag="VW343_000001"
     CDS             491..1009
                     /locus_tag="VW343_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9754635.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            1139..1645
                     /locus_tag="VW343_000002"
     CDS             1139..1645
                     /locus_tag="VW343_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9754636.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            1645..1815
                     /locus_tag="VW343_000003"
     CDS             1645..1815
                     /locus_tag="VW343_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754637.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            1847..3079
                     /gene="clpX"
                     /locus_tag="VW343_000004"
     CDS             1847..3079
                     /gene="clpX"
                     /locus_tag="VW343_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9754638.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            3088..3675
                     /locus_tag="VW343_000005"
     CDS             3088..3675
                     /locus_tag="VW343_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9754639.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            3686..4066
                     /locus_tag="VW343_000006"
     CDS             3686..4066
                     /locus_tag="VW343_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9754640.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            4118..5008
                     /gene="rapZ"
                     /locus_tag="VW343_000007"
     CDS             4118..5008
                     /gene="rapZ"
                     /locus_tag="VW343_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9754641.1"
                     /translation="MTKKQFHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            5005..5982
                     /locus_tag="VW343_000008"
     CDS             5005..5982
                     /locus_tag="VW343_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9754642.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            5979..6890
                     /gene="whiA"
                     /locus_tag="VW343_000009"
     CDS             5979..6890
                     /gene="whiA"
                     /locus_tag="VW343_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9754643.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(6978..8105)
                     /locus_tag="VW343_000010"
     CDS             complement(6978..8105)
                     /locus_tag="VW343_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9754644.1"
                     /translation="MWMEELPNGKFKYFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKINKKLSTKQVESITFEEIYNLFYKSWAQTVKESTKHNCKSVDKKMKEVIPSD
                     TILANLDRRFLQEAIEKIIESNGYITAKKVRHRLRGIFNYAVQYSYIENNEVDYTTIP
                     QKPKTLEELEKKRNNFLTMQEIKALVDVLNRREYHQKYADMVLVLTLTGMRYGELTAL
                     QLKNIDFENNKIEITGNFDSVNKIKTLPKTTNSIRTIKVSESVIEAIQRQIVRLSERF
                     QPLSSDDYIFCFEKWNQPTTIACFIQILKKYGKQAKIEKNLSSHIFRHSHISFLAESD
                     LPIKSIMDRVGHSNAKMTLEIYSHTTEDMEDKLVNKLDTIF"
     gene            complement(8384..9334)
                     /locus_tag="VW343_000011"
     CDS             complement(8384..9334)
                     /locus_tag="VW343_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEU9754645.1"
                     /translation="MKPFKTIEEQIATLKIRGLSITDESKAAKYLLSNNYYNIINGYS
                     KFFQHPGTDTYIDGVTFDEVSSLYTFDKDVKRAILQAILEAEHHIKSITAHRFAEAYP
                     SQKYAYLNTNSYADNKILDVGFIVSKLSKIINTNKRYRGNSIHHYAHTHSDVPIWVLT
                     DYLEFGDLRTIIENLPNSLQNEIARDLVSFISTNIPDFNDVFPPETLISFLKNINEVR
                     NKCAHNNRLLNFRCRSNSTFWETIHNKEILMGDDSRKTVYSTIISLQCFISKAAFNIL
                     WNTLRKKVIKLEKKLPSIDINVINQSLGFPNDWHRNEPKV"
     gene            complement(9483..10274)
                     /locus_tag="VW343_000012"
     CDS             complement(9483..10274)
                     /locus_tag="VW343_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9754646.1"
                     /translation="MDEKKRMQIIAENITHFRKQRGITQKELAKEVGITASTMTDYMK
                     LRSAPSFGVIQKLADYFGVKKSDIDTTFKEESTNSLPDAPDSLTQQIMDKVVQLTPPN
                     QKIVLRTSEELLESQNEEETKINEVSEVISLYQVEVVSETAAASGFNYGFGYDDTDRE
                     TIEVDERPPRHDIATKVSGDSMQPDYQDGDILYLVDKGLTTYNGDLAIIAYGDRSYFK
                     KIYTENGRLRLVSLNDKYEDIILDFPPAEDTHIKIYAVVGVYRGE"
     gene            complement(10478..11167)
                     /locus_tag="VW343_000013"
     CDS             complement(10478..11167)
                     /locus_tag="VW343_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEU9754647.1"
                     /translation="MIFRVKVNSSVSRPVTVEDICPNCKKPTNPDVVNSSYFSLGENK
                     TSLVLTCRCLGCKHFWTEEFIATRFLINDYTEKYEIEHIKVIPNLPSDIPISDDVEIV
                     SPIGKQIYVQALKAEHEQLDHIAGIGYRKALEFFVKDFSIVTNPDDEDKIIKMSLKQV
                     IEKYIKDEDLKTFALASAYIGNDEGHYYRNNPDKGFTDLKNYLHGVIHYMEMKLNFLD
                     AQELVNRSKKS"
     gene            11374..11538
                     /locus_tag="VW343_000014"
     CDS             11374..11538
                     /locus_tag="VW343_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754648.1"
                     /translation="MKENIEHLHERIKHFQSLIAYMSEHEKKYYLEKDWFDNPTLISI
                     EDAKKEVELL"
     gene            11557..11772
                     /locus_tag="VW343_000015"
     CDS             11557..11772
                     /locus_tag="VW343_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9754649.1"
                     /translation="MQWTLEAMRINKGLTQAELAEKFEVSSQTIARLEKDSSDIGYQL
                     LKKYMFFFNVKFDDIFLGKKYENFVNN"
     gene            11804..12004
                     /locus_tag="VW343_000016"
     CDS             11804..12004
                     /locus_tag="VW343_000016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754650.1"
                     /translation="MESVLESLKQEKDHLEKIIKVVTSGGKFLRLPYQKKSRSISENL
                     KLISQNLDKLSEQVQQTTNQHS"
     gene            complement(12403..12594)
                     /locus_tag="VW343_000017"
     CDS             complement(12403..12594)
                     /locus_tag="VW343_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754651.1"
                     /translation="MKSFKDFRESLTAEDMQAISAKANEATKQIDHTDGLQLGKVSGL
                     TSVITTIELLEKYHEWLHS"
     gene            12713..12856
                     /locus_tag="VW343_000018"
     CDS             12713..12856
                     /locus_tag="VW343_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754652.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     RTV"
     gene            12853..13023
                     /locus_tag="VW343_000019"
     CDS             12853..13023
                     /locus_tag="VW343_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754653.1"
                     /translation="MKEAVKEFLKFRSRFTKIEWFEINQAVEARLNQKADQLKLDDVD
                     LEIISSRLEKVI"
     gene            complement(13016..13255)
                     /locus_tag="VW343_000020"
     CDS             complement(13016..13255)
                     /locus_tag="VW343_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754654.1"
                     /translation="MTEIHACLCGNWVNLSADDDCVMGPNMASPYIWWEENAELYSPI
                     SKPEANSMYHQDYIYIHYRGADYRIHPMFIQIVSR"
     gene            13373..13645
                     /locus_tag="VW343_000021"
     CDS             13373..13645
                     /locus_tag="VW343_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754655.1"
                     /translation="MEAVEIVRIKDVIIEKVSANDEELKRIFGCSKRQAGERRREMQK
                     LPSQQKHLLDSGQLVTIKGFYEYLQYRGTKAWKKEMETSKKMRSAG"
     gene            13642..13857
                     /locus_tag="VW343_000022"
     CDS             13642..13857
                     /locus_tag="VW343_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754656.1"
                     /translation="MNLLSRIKNYFSEEVKETNLDWKEVALDLNQSLIETQEKLQEAN
                     QEIADLKKIVAIYKEKEKEKRWNIFTW"
     gene            13836..14024
                     /locus_tag="VW343_000023"
     CDS             13836..14024
                     /locus_tag="VW343_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754657.1"
                     /translation="MEYIYLVTIVGIGLWSLVNKLDDHAEMKQKERQLMANNVARMNL
                     RNSDKQFTYDVEPPEGLK"
     gene            14036..14377
                     /locus_tag="VW343_000024"
     CDS             14036..14377
                     /locus_tag="VW343_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754658.1"
                     /translation="MTQAERIREYYREHPAASYDEVAEVVGTTNSNVRANLAKDIKAG
                     RCVRLEDKSYDYSPYYNHTQALTELVDWKNDNRREWVDMLTRAAEKETDSNVMRLLIK
                     EANKLMKEVTK"
     gene            14378..14608
                     /locus_tag="VW343_000025"
     CDS             14378..14608
                     /locus_tag="VW343_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754659.1"
                     /translation="MVRNKIGDLTNTLFAQLETLDDRDLTADELKVELHRSKQMVAIS
                     GQILQAGQLALDAEKFKDKVGEVNAPIALLEG"
     gene            14622..15344
                     /locus_tag="VW343_000026"
     CDS             14622..15344
                     /locus_tag="VW343_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU9754660.1"
                     /translation="MHEICPGRLTPEVTRLLNEKFGTNYNKSQIGGVRKRLGLAVGKV
                     YQGRLLTKEQHDYLVLIQKNKISRNVANEMNQKFGLSLTEKQIKSYRRNNNLHSGLTG
                     RFEKGQTPHNKGKKYPNMPKNGGQFKKGNRPPNYVPVGTINYTTNGYPKEKIGEPNQW
                     VLKHRKVWEDHHGLIPKGYSIVFLDGDKTNYDISNLACLSKNEIARMNQNHLFTSNAD
                     LTKSGIGLTKLTNKIREVEKNG"
     gene            15337..15819
                     /locus_tag="VW343_000027"
     CDS             15337..15819
                     /locus_tag="VW343_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEU9754661.1"
                     /translation="MASLYELTGQFLTIYQLDIDDETKADTLEAIDWQEQFEQKAEGY
                     AHVIKNLEADVAMYKAEEESFKAKKQVAQKKLDYVKDNIMAAMNVTGQTEVKSGALII
                     KIAKNPESVKVNEDDLPKKYFTKKVTLAPDKKTLKELLKSGKKVKGAELVRTEKLVIK
                     "
     gene            15819..16178
                     /locus_tag="VW343_000028"
     CDS             15819..16178
                     /locus_tag="VW343_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754662.1"
                     /translation="MELMNKTRVTDSLAVVIGPESIEVLVTEGFLFDVAIRFVKVDET
                     NLDQGNEKPVFTPEYKLVTVAKYKEKPIFESEEDIRKFEKQAKEVKSLFAFAKVNKQN
                     WFNTALYPGVLTEKVGV"
     gene            16178..16663
                     /locus_tag="VW343_000029"
     CDS             16178..16663
                     /locus_tag="VW343_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754663.1"
                     /translation="MKILAIDPSSNKIETSTTGVVLLDNARLVDSWVVSYGMRGFADW
                     FHEIGTNLEFDVVIVEEFKARDNDKSKDNSVAETIAYIQLCYPGAILQFNAGYKSDIP
                     NDLLKILDLWKFEKSHHQDIRAAARLGLFWAMRNDIEEVVHDIGKVVSEYHNNAKKVA
                     S"
     gene            16626..17819
                     /locus_tag="VW343_000030"
     CDS             16626..17819
                     /locus_tag="VW343_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HEU9754664.1"
                     /translation="MSITITLRKWQAEAIKRSEHLSNGIFLEALGGRGKTICALAIAK
                     HKKAKKIIITNNRLAILNGWIDAVKFMNFDKGVEIIIQTDRYLQNQVKKGHKLDCDVL
                     IVDEWQNMSSDKQVALYRKIKRKYTIGLSATPIRKKGQNFYPLEKTVFGWATPNNKFD
                     WQKTHGKMVYDPFSYSKEKWEDFQNYESYISSLPNFFRWEEIEGIENAVENNGFEIKF
                     YQKRVASGNPEKLAEFRKLNLVTVDGKTAMAKQSFGRKTFERYLNQTGVAVDFPKLKP
                     VNADTPLMLQLDGLIERAPHDMLIVSKSKQIVNVISERHPEIGIWTGDIQEGLYKKFV
                     VATSQVLGVGVDGLQHKYQTIVVLDPVEEGSGEYDDYRQLLWRITGSRQQHDVNVIEF
                     YYKES"
     gene            17837..18154
                     /locus_tag="VW343_000031"
     CDS             17837..18154
                     /locus_tag="VW343_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754665.1"
                     /translation="MNKTTEMIVFRSRKTGEFLNSYKDRSSLAFAADFCSLEYCLKLP
                     RKKYEDNKKTYKALAAAFDCEIVAVEAEYKLTYPNGSEVEPIKRDRSSIEDMIKDIIG
                     GVL"
     gene            18154..18981
                     /locus_tag="VW343_000032"
     CDS             18154..18981
                     /locus_tag="VW343_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9754666.1"
                     /translation="MAFTLPANKPQVPKDTPRNFFIYGETMSGKSYLANEFPNPIVLN
                     TDGNAEANTVPSIQLINEKDDKGRITNSVIKQLGDILLALQTQKHSYETVVIDVIDDV
                     IEMIKIAVCDELTPVGKPRLKSLSEIPYGKGYDFFNQAITELVIDLKALPMNVIYISR
                     QVSEYDDNGNATKDKPSLKDKYVNLINGNSDLMIHTEKLGNNYNREVDRKRKTYYADQ
                     VDDKAILKILATIRGAVEPAKGKLAPKKEAAKTTKPAKTEKTKEAPKKEVDSDDELF"
     gene            19001..19507
                     /locus_tag="VW343_000033"
     CDS             19001..19507
                     /locus_tag="VW343_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754667.1"
                     /translation="MSLLDIAKSIKKEGFDPRKDSANGPAPIPAGTYPVVLKKATFNV
                     SDKGWESLGYQFEIRGGDYSGRSEFATFGTLTEWNGKNLDWAVERTMKFFIKALVLAG
                     DSMQGNEEDGKALEEALQRKAVGSYYNLVISVTKGKDGREFRNYDLEEEEAQPLTEAD
                     IDEDDLPF"
     gene            19680..20501
                     /locus_tag="VW343_000034"
     CDS             19680..20501
                     /locus_tag="VW343_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEU9754668.1"
                     /translation="MPSMKEYALQYQKLGFSVIPINPKNKMPLIEFADKPAMTPSEIE
                     NFWDGYPNANIALKTTNFFVIDIDKHGKSNGFESLKKWKHLNLIEPTLQAKTASGGKH
                     LFYFKREDEPITQMIGFLPGVDIKAHENNYVLVAPSATDKGQYEWDLEKSKEGGTMVT
                     PSKYLIQSIKKQYGETHGYKYDGKDGLRDLVRRSHTRDRTQTTDLFETIALGFGDEGG
                     RNDKLAKFVGGLLYRAVDDGVVVQLARLANANSPTPLPEKEMMRTIESMIKKDRR"
     gene            20504..21847
                     /locus_tag="VW343_000035"
     CDS             20504..21847
                     /locus_tag="VW343_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9754669.1"
                     /translation="MIGNVVSIDSQPKMITTAKGDIKANSPSNVLMSFKADDQLSIYL
                     KHNDFSQEHELLKDIKIGNTLFKKGELPSNFDSVVKVYFESVLGVAFSSQAMLDGMET
                     FFSERSYNPVIEYMEKAAEKWDGRKRIDRMLQVYLGAEDIPLVSKIAQMWLVGAVAKV
                     YDPYVKFDYVLDLVGGQGVGKTSLLQKLGGEWYTDAVTDFSNKDNYDIMLKSLIVNDD
                     EMVASNRMSFAETKAFISKTSLRYRKPYMKRTEEFAKNFILARTTNQTEYLKDKTGER
                     RFLPVMADSKRQKKHPMEIEPETIEQIWGEAVTIYRAGADLMFDENTEDELNIYREQF
                     MYRDEVELQVLEYLDMPVPENWQNWSIQQQHQYTSKYFDNSSDFDPGSKKLDKVSTRE
                     MMYNLFMRNSNDRKLSTKINMIMDNHPDWKKSVFRAGGKSTKGFVRVKDSEKTNR"
     gene            22140..22295
                     /locus_tag="VW343_000036"
     CDS             22140..22295
                     /locus_tag="VW343_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754670.1"
                     /translation="MSYTVTLFFDNMVDETHFFKKESDAAKCKAQLESKYRGNRMYKV
                     KQEKLEE"
     gene            22292..22987
                     /locus_tag="VW343_000037"
     CDS             22292..22987
                     /locus_tag="VW343_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9754671.1"
                     /translation="MNKQELIKRIEDLPYTEGPIADTIEINRNWILKSIEQLAESEIG
                     HADEAPRYVKNILARLRELPLHDREFWLKAIMSEFEQDFSHAKWREGYEQGKIEGMVE
                     REKVIVPQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLDG
                     YEVEKEKRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELENAKIGW
                     VFDCEGFEIEEVE"
     gene            23145..23405
                     /locus_tag="VW343_000038"
     CDS             23145..23405
                     /locus_tag="VW343_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEU9754672.1"
                     /translation="MKRFIAIWILLSAGLNIWQSIYIKKLEEKRPIVVYKADNAGAEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYEKVKVGDEVLL"
     gene            23405..23863
                     /locus_tag="VW343_000039"
     CDS             23405..23863
                     /locus_tag="VW343_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754673.1"
                     /translation="MDDILQALAKMLNMTVDEVSSLLTTFKGNAPQIYEMFVKEKMFY
                     DLFSLFQIMSIVIFSISAVVLAVLTLIYFTYDGGFVYSYDIRTGKTEEEIKLERIERK
                     RKDLKIPLKISCISSSASLITLVIAIVLKATLAPNYIFIVNEILPKLTKR"
     gene            23911..23979
                     /locus_tag="VW343_000040"
                     /pseudo
     CDS             23911..23979
                     /locus_tag="VW343_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            23976..24413
                     /locus_tag="VW343_000041"
     CDS             23976..24413
                     /locus_tag="VW343_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754674.1"
                     /translation="MIPKFRVWVKIGKRMVFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYDFDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYDKEFIG
                     IVKQLEGSWVIDTGSDAVCLWTEIEENEIIGNIYENKEFGGRK"
     gene            24410..24781
                     /locus_tag="VW343_000042"
     CDS             24410..24781
                     /locus_tag="VW343_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754675.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFKH"
     gene            24854..25258
                     /locus_tag="VW343_000043"
     CDS             24854..25258
                     /locus_tag="VW343_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9754676.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSEVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            25339..25421
                     /locus_tag="VW343_000044"
     tRNA            25339..25421
                     /locus_tag="VW343_000044"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25370..25372,aa:Tyr,seq:gta)
     gene            25688..26167
                     /locus_tag="VW343_000045"
     CDS             25688..26167
                     /locus_tag="VW343_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEU9754677.1"
                     /translation="MAFFDYSKGGDGKLSGLRIKQKRFADEYIISGNATEAYKKAGYR
                     VSSDRVAGVEGHKLLKNPKIKSYIDERLKQLDSEKIADQQEVLGYLTSVMRGETQEQT
                     LISIGELGQTITDIDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDISD"
     gene            26145..27443
                     /locus_tag="VW343_000046"
     CDS             26145..27443
                     /locus_tag="VW343_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU9754678.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDIGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            27455..28924
                     /locus_tag="VW343_000047"
     CDS             27455..28924
                     /locus_tag="VW343_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9754679.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHNTESSRLKNLKSYYM
                     GDHAILNHKRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            28833..29864
                     /locus_tag="VW343_000048"
                     /pseudo
     CDS             28833..29864
                     /locus_tag="VW343_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            30224..30451
                     /locus_tag="VW343_000049"
     CDS             30224..30451
                     /locus_tag="VW343_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HEU9754680.1"
                     /translation="MAILDDLQALYDNGWDASFNYNGQVCGIFPNSIYDVVVIIADDE
                     YRASSFDDLISLQIEGKTLPEIMNEVEVQYG"
     gene            30600..30812
                     /locus_tag="VW343_000050"
     CDS             30600..30812
                     /locus_tag="VW343_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754681.1"
                     /translation="MEEWKERFKKEYYELKERFQKLDMMIGKYEKGQLEFESKCPIDS
                     LKGQRSTMWNYLRILEQRAKIEEIKL"
     gene            30982..31566
                     /locus_tag="VW343_000051"
     CDS             30982..31566
                     /locus_tag="VW343_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEU9754682.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDSVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPV
                     DSDGITKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            31573..32418
                     /locus_tag="VW343_000052"
     CDS             31573..32418
                     /locus_tag="VW343_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEU9754683.1"
                     /translation="MTAGQTKLATMVNPEVMADMVAAKLPKLIKFTPLAYVETKLEGQ
                     PGSTLTVPAWEYAGDATEIEEGQAITPDQLTTKKTTMTIKKAGKGYEITDESLLSGLG
                     DPLGQATYQLGLAIANKIDNDLVAVAKTATQHITETPTTLEAIDKALDIFEDEEDAQY
                     VAIINPKDATKLKTAVAKEWIKGSELGANMVVSGTFGEVDGVQIVRSKKVDEGKGFLV
                     KVSPSQTQTDDANKYGAFVILLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGGA"
     gene            32430..32612
                     /locus_tag="VW343_000053"
     CDS             32430..32612
                     /locus_tag="VW343_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEU9754684.1"
                     /translation="MSLLLRRHYIQEEQVNQYSDLENKTLEELKDLAKEAGVAGAYKL
                     TKAEIVEVLEELKSEI"
     gene            32602..32790
                     /locus_tag="VW343_000054"
     CDS             32602..32790
                     /locus_tag="VW343_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754685.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVT
                     ISDVLEKILPEPEFLEED"
     gene            32792..33103
                     /locus_tag="VW343_000055"
     CDS             32792..33103
                     /locus_tag="VW343_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU9754686.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTDLLQRIRMHRLARVAGHVFEA
                     E"
     gene            33084..33422
                     /locus_tag="VW343_000056"
     CDS             33084..33422
                     /locus_tag="VW343_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754687.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            33397..33912
                     /locus_tag="VW343_000057"
     CDS             33397..33912
                     /locus_tag="VW343_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU9754688.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            33909..34277
                     /locus_tag="VW343_000058"
     CDS             33909..34277
                     /locus_tag="VW343_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754689.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGIVDLHSELVFQFRMEN"
     gene            34280..34693
                     /locus_tag="VW343_000059"
     CDS             34280..34693
                     /locus_tag="VW343_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9754690.1"
                     /translation="MLANGITLSYGETKDSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYEFGIGDIGELEYKFSYNNSSATAPYRVLRKAADDKKKLYFEQAYPDGTKVIFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSNLTFTDGIGG"
     gene            34698..35078
                     /locus_tag="VW343_000060"
     CDS             34698..35078
                     /locus_tag="VW343_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754691.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSLEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVDK"
     gene            35117..35467
                     /locus_tag="VW343_000061"
     CDS             35117..35467
                     /locus_tag="VW343_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754692.1"
                     /translation="MFLDIEGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            35454..39107
                     /locus_tag="VW343_000062"
     CDS             35454..39107
                     /locus_tag="VW343_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754693.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSSSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIERIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNIGNISEAVGNIAQDFSSTFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVAPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPILALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSLIVGGIKNLGLAIKALTFDKLVSFGETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICRAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTSALANIGAWFTDIFQKAWTGLTNIFSKLGLWFGERWADVTSVLANVSSWFGN
                     MFTSAYNAVKNAFSSIGGFFSGVWSTVQSIFVNAGQKVGSAVGGAFKSAVNAVLGTIE
                     NVVNDFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEA
                     VVPLENTGFIQTLGRVVSSAVVNAMAGISPQGGFSSDGDIVIQIAGHEFGRVAIQEIN
                     KEHERAGQTLLKI"
     gene            39116..39466
                     /locus_tag="VW343_000063"
     CDS             39116..39466
                     /locus_tag="VW343_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754694.1"
                     /translation="MAQLTINGVAVKPPKSFQVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSKFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     LKPWSGAKFNLVER"
     gene            39471..45086
                     /locus_tag="VW343_000064"
     CDS             39471..45086
                     /locus_tag="VW343_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEU9754695.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGEYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMTTGRSLELDNVFITQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFIFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGFEAKIQAQIEAADKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKEAKRKAEEALRNAGASSSLAQESKRIGLDSVARLEAFKSQ
                     TTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEALSRTKNELDGASTLLAQE
                     AKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEA
                     LSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELASRIASVQA
                     SGRNLFLNSLFKQDISKTGIWTTSTYTATIDSESKYLGYNALKIIGLNPSGRDGGNPK
                     VTYPVLGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIK
                     RYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVDTSYSEAPEDIEGQI
                     STVESTFKQRVNSLEAGVNRLTEGLRTKADISALNVTAENIRQSVKSLETDTQNKLNQ
                     KLSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKMKVGGRNLWIKSKT
                     VGAVIEKLPENHVTGQKECYRLENNSTLTFNLEPDFSSRLYQKVTFSAWIKYENVVQG
                     RNFWNVFNCFKHYLFRKNSETGVQSGPDYATLGMYKGSADWKYITFTYDYSEKTNFDQ
                     LKTSLRFNLEGATSGTAWVTGIKVEIGSVATDWSPAPEDGENELLVAKTEFKRTADGL
                     STKMAAVESYVGQDGQRQEALQRYTREESTRQATAVRELVNRDFVGKATYQEDVKGIN
                     QRIEAVKTSANKDIASQIASYRQSVDGKFTDISSQITTYKQDVGGQISGLSNRLTSSE
                     QGTTTQISNISNRINSNKQGTDNQISNLKTQVATNKDNAERQMGRISDQVSANKANAD
                     SQFANVTNQLARKVETTDFQRVKETSKLYERILGNTENGIADKVARMALTNQLFQVEV
                     GKYSVSGPNLIKNSDFKNATNEWGSTQNLGRLVKHSFYHNGQKDLMRLSNATKNENFL
                     YSHRFNLERNTDYVLNFRGFNNSALASYDVYILGRRAGESDGFTIVKKVVSSKKLSTS
                     RCEDVSVTFNSGEMDNAYIRFDNNGSSSGTADLYITEVDLYKGYKPRTWQPHPEDAVA
                     DANKKLEATQTKMTQLAGSWVVENINSAGDIISGINLGANGHNRFVGKLTHITGETLI
                     DRAVIKSAMVDKLKTGNFEAGSVTTTILDAEAVTAEKLKVDDALIRKLTANDAFIDRL
                     TSKRIFSTKVESVISSSTFLEAYQGRIGGFTLGQFDQGGGRWISGVNQFSVGMGNGAG
                     HGVRTAFWANWGNNWNYAGPKAWNVNTDGKMYCRNEVGFYDQVDFSNSSRANFYGNTT
                     FSRSPVFSNGIELGSKDVLGDGWNPKGGRNAVVWWNQVGSGSVKYWMEQKSDRRLKEN
                     ITDTAVKALDKINRLRMVAFDFIENKKHEEIGLIAQEAETIVPRIVSRDPENPDGYLH
                     IDYTALVPYLIKAIQELNQKIEKMEKTIA"
     gene            45181..45384
                     /locus_tag="VW343_000065"
     CDS             45181..45384
                     /locus_tag="VW343_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754696.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITNNILSIQLDEQREGNQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            45387..45737
                     /locus_tag="VW343_000066"
     CDS             45387..45737
                     /locus_tag="VW343_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754697.1"
                     /translation="MAETNQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            45747..46046
                     /locus_tag="VW343_000067"
     CDS             45747..46046
                     /locus_tag="VW343_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754698.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHAVGDIQKEMVRMNDRMEEIEKGE"
     gene            46050..46382
                     /locus_tag="VW343_000068"
     CDS             46050..46382
                     /locus_tag="VW343_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9754699.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            46386..47342
                     /locus_tag="VW343_000069"
     CDS             46386..47342
                     /locus_tag="VW343_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9754700.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLRAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKNTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPDFTVEPDGLITVK"
     gene            complement(47594..48562)
                     /locus_tag="VW343_000070"
     CDS             complement(47594..48562)
                     /locus_tag="VW343_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9754701.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELESVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFVPSQLLGDGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            48698..49555
                     /locus_tag="VW343_000071"
     CDS             48698..49555
                     /locus_tag="VW343_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9754702.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            49542..50321
                     /locus_tag="VW343_000072"
     CDS             49542..50321
                     /locus_tag="VW343_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9754703.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            50345..51697
                     /locus_tag="VW343_000073"
     CDS             50345..51697
                     /locus_tag="VW343_000073"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9754704.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            51771..52145
                     /locus_tag="VW343_000074"
     CDS             51771..52145
                     /locus_tag="VW343_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9754705.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            52147..52995
                     /locus_tag="VW343_000075"
     CDS             52147..52995
                     /locus_tag="VW343_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9754706.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASEFPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            53272..54039
                     /locus_tag="VW343_000076"
     CDS             53272..54039
                     /locus_tag="VW343_000076"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9754707.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            54051..55235
                     /locus_tag="VW343_000077"
     CDS             54051..55235
                     /locus_tag="VW343_000077"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9754708.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDLDLLLQAENLRQAQGKEVNPQVIKETYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            55232..57103
                     /locus_tag="VW343_000078"
     CDS             55232..57103
                     /locus_tag="VW343_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9754709.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLTTLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(57246..58430)
                     /gene="mntE"
                     /locus_tag="VW343_000079"
     CDS             complement(57246..58430)
                     /gene="mntE"
                     /locus_tag="VW343_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9754710.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            58734..61430
                     /locus_tag="VW343_000080"
     CDS             58734..61430
                     /locus_tag="VW343_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9754711.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            61443..62234
                     /gene="yghU"
                     /locus_tag="VW343_000081"
     CDS             61443..62234
                     /gene="yghU"
                     /locus_tag="VW343_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9754712.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTESVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            62350..62760
                     /locus_tag="VW343_000082"
     CDS             62350..62760
                     /locus_tag="VW343_000082"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9754713.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            62772..64367
                     /locus_tag="VW343_000083"
     CDS             62772..64367
                     /locus_tag="VW343_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754714.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            64434..64598
                     /locus_tag="VW343_000084"
     CDS             64434..64598
                     /locus_tag="VW343_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9754715.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            64677..65405
                     /gene="yaaA"
                     /locus_tag="VW343_000085"
     CDS             64677..65405
                     /gene="yaaA"
                     /locus_tag="VW343_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9754716.1"
                     /translation="MKILIPTAKEMNTDLPSIEAISLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTTQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(65682..66230)
                     /locus_tag="VW343_000086"
     CDS             complement(65682..66230)
                     /locus_tag="VW343_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9754717.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            66551..67039
                     /locus_tag="VW343_000087"
     CDS             66551..67039
                     /locus_tag="VW343_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9754718.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            67036..68223
                     /locus_tag="VW343_000088"
     CDS             67036..68223
                     /locus_tag="VW343_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9754719.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            68216..68581
                     /locus_tag="VW343_000089"
     CDS             68216..68581
                     /locus_tag="VW343_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9754720.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            68597..69943
                     /gene="asnS"
                     /locus_tag="VW343_000090"
     CDS             68597..69943
                     /gene="asnS"
                     /locus_tag="VW343_000090"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9754721.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVKNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            70096..70386
                     /locus_tag="VW343_000091"
     CDS             70096..70386
                     /locus_tag="VW343_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9754722.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            70398..70868
                     /gene="ssbA"
                     /locus_tag="VW343_000092"
     CDS             70398..70868
                     /gene="ssbA"
                     /locus_tag="VW343_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9754723.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            70900..71139
                     /locus_tag="VW343_000093"
     CDS             70900..71139
                     /locus_tag="VW343_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9754724.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            71434..72834
                     /locus_tag="VW343_000094"
     CDS             71434..72834
                     /locus_tag="VW343_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9754725.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSIRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            72836..73195
                     /locus_tag="VW343_000095"
     CDS             72836..73195
                     /locus_tag="VW343_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9754726.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEKKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            73249..73998
                     /locus_tag="VW343_000096"
     CDS             73249..73998
                     /locus_tag="VW343_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9754727.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            73988..74287
                     /locus_tag="VW343_000097"
     CDS             73988..74287
                     /locus_tag="VW343_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9754728.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            74358..75293
                     /locus_tag="VW343_000098"
     CDS             74358..75293
                     /locus_tag="VW343_000098"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9754729.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRIITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            75379..75891
                     /locus_tag="VW343_000099"
     CDS             75379..75891
                     /locus_tag="VW343_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9754730.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERDLHDSDF"
     gene            75916..76524
                     /locus_tag="VW343_000100"
     CDS             75916..76524
                     /locus_tag="VW343_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9754731.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDRKYELAIDFDKERLTFYLA"
     gene            76625..76819
                     /locus_tag="VW343_000101"
     CDS             76625..76819
                     /locus_tag="VW343_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9754732.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(76865..78310)
                     /locus_tag="VW343_000102"
     CDS             complement(76865..78310)
                     /locus_tag="VW343_000102"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9754733.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMEKSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            78416..80038
                     /locus_tag="VW343_000103"
     CDS             78416..80038
                     /locus_tag="VW343_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9754734.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            80241..82199
                     /locus_tag="VW343_000104"
     CDS             80241..82199
                     /locus_tag="VW343_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9754735.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            82371..83306
                     /locus_tag="VW343_000105"
     CDS             82371..83306
                     /locus_tag="VW343_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9754736.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTIYSRSKGSSVALENLLAVPKEKFE"
     gene            83331..84053
                     /locus_tag="VW343_000106"
     CDS             83331..84053
                     /locus_tag="VW343_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9754737.1"
                     /translation="MNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPSGSGKSTIV
                     RLLERFYKPLSGYILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRL
                     VTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEIL
                     IFDESTANLDADSEYAIISILYSALKEKTVVIIAHRLSTVKDVDCIFFLEGGKITGSG
                     THKELLENHERYARFVQEQMIE"
     gene            84211..85305
                     /locus_tag="VW343_000107"
     CDS             84211..85305
                     /locus_tag="VW343_000107"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9754738.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDTRDLIADLRQALEG"
     gene            85313..86479
                     /locus_tag="VW343_000108"
     CDS             85313..86479
                     /locus_tag="VW343_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9754739.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVVTFAKR"
     gene            86572..89670
                     /locus_tag="VW343_000109"
     CDS             86572..89670
                     /locus_tag="VW343_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9754740.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVVVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            89718..90335
                     /locus_tag="VW343_000110"
     CDS             89718..90335
                     /locus_tag="VW343_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9754741.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVQPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            90346..91680
                     /locus_tag="VW343_000111"
     CDS             90346..91680
                     /locus_tag="VW343_000111"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9754742.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEVEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            91690..92154
                     /locus_tag="VW343_000112"
     CDS             91690..92154
                     /locus_tag="VW343_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9754743.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            92151..92645
                     /locus_tag="VW343_000113"
     CDS             92151..92645
                     /locus_tag="VW343_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9754744.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            92726..94381
                     /gene="mltG"
                     /locus_tag="VW343_000114"
     CDS             92726..94381
                     /gene="mltG"
                     /locus_tag="VW343_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9754745.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGSETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            94452..94934
                     /gene="greA"
                     /locus_tag="VW343_000115"
     CDS             94452..94934
                     /gene="greA"
                     /locus_tag="VW343_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9754746.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            95216..95614
                     /locus_tag="VW343_000116"
     CDS             95216..95614
                     /locus_tag="VW343_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9754747.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAL
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            96035..97042
                     /locus_tag="VW343_000117"
                     /pseudo
     CDS             96035..97042
                     /locus_tag="VW343_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            97117..97317
                     /locus_tag="VW343_000118"
     CDS             97117..97317
                     /locus_tag="VW343_000118"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9754748.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            97352..98068
                     /gene="atpB"
                     /locus_tag="VW343_000119"
     CDS             97352..98068
                     /gene="atpB"
                     /locus_tag="VW343_000119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9754749.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNILEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            98082..98576
                     /gene="atpF"
                     /locus_tag="VW343_000120"
     CDS             98082..98576
                     /gene="atpF"
                     /locus_tag="VW343_000120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9754750.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            98576..99112
                     /locus_tag="VW343_000121"
     CDS             98576..99112
                     /locus_tag="VW343_000121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9754751.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            99127..100632
                     /locus_tag="VW343_000122"
     CDS             99127..100632
                     /locus_tag="VW343_000122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9754752.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            100648..101526
                     /locus_tag="VW343_000123"
     CDS             100648..101526
                     /locus_tag="VW343_000123"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9754753.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            101612..103018
                     /gene="atpD"
                     /locus_tag="VW343_000124"
     CDS             101612..103018
                     /gene="atpD"
                     /locus_tag="VW343_000124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9754754.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            103029..103448
                     /locus_tag="VW343_000125"
     CDS             103029..103448
                     /locus_tag="VW343_000125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9754755.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(103493..104506)
                     /locus_tag="VW343_000126"
     CDS             complement(103493..104506)
                     /locus_tag="VW343_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9754756.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            104650..105816
                     /locus_tag="VW343_000127"
     CDS             104650..105816
                     /locus_tag="VW343_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9754757.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFIAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            105806..107035
                     /locus_tag="VW343_000128"
     CDS             105806..107035
                     /locus_tag="VW343_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9754758.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            107181..107822
                     /locus_tag="VW343_000129"
     CDS             107181..107822
                     /locus_tag="VW343_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9754759.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            107832..108461
                     /locus_tag="VW343_000130"
     CDS             107832..108461
                     /locus_tag="VW343_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9754760.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            108474..109310
                     /locus_tag="VW343_000131"
     CDS             108474..109310
                     /locus_tag="VW343_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9754761.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(109589..109936)
                     /locus_tag="VW343_000132"
     CDS             complement(109589..109936)
                     /locus_tag="VW343_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9754762.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFVGL"
     gene            complement(110047..111765)
                     /locus_tag="VW343_000133"
     CDS             complement(110047..111765)
                     /locus_tag="VW343_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9754763.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            111999..112324
                     /locus_tag="VW343_000134"
                     /pseudo
     CDS             111999..112324
                     /locus_tag="VW343_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            112397..112843
                     /locus_tag="VW343_000135"
     CDS             112397..112843
                     /locus_tag="VW343_000135"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9754764.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERISIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            113183..113437
                     /locus_tag="VW343_000136"
     CDS             113183..113437
                     /locus_tag="VW343_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754765.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            113548..114654
                     /locus_tag="VW343_000137"
     CDS             113548..114654
                     /locus_tag="VW343_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9754766.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            114612..115220
                     /locus_tag="VW343_000138"
     CDS             114612..115220
                     /locus_tag="VW343_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9754767.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            115444..116313
                     /locus_tag="VW343_000139"
     CDS             115444..116313
                     /locus_tag="VW343_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9754768.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            117224..118420
                     /gene="tuf"
                     /locus_tag="VW343_000140"
     CDS             117224..118420
                     /gene="tuf"
                     /locus_tag="VW343_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9754769.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT        36650 a        21632 c        26702 g        33795 t
ORIGIN      
        1 atttgaaaga gggatgtgat agtagcaccc tctttttctt tgttttatga tagttcatcc
       61 tcgagtaaat tcaagatctt gctcggaaat gaagcttata tagtagattg aatatagaca
      121 aagaccttgt gattggtaaa acattttaga aattcattta cctttcctaa tcgacttggt
      181 ttcatcttat ttcaatctat tatagtattg gggaatttct tcaaaccaca tcagcttggt
      241 cagttctacc tgcgacctca aaacttgtgc tttggtcaag ctgggtttag tttcctagtt
      301 tgctgatgga tttccattga ctataagcat ccaaccctct tttttgtctt ctaaagaatt
      361 cttaaattat cagtctattg caacttttct catataagtt ctttgtcttg ctattggttt
      421 tccttagtag tatactaagg tagtaatcat taagaagtgg ttacaaaaaa taatgaatga
      481 ggtaaagaaa atggtagaat tgaaaaaaga agcagtaaaa gacgtaacat cattgacaaa
      541 agcagcgcca gtagcattgg caaaaacaaa ggaagtcttg aaccaagctg ttgctgattt
      601 gtatgtagct cacgttgctt tgcaccaagt gcactggtat atgcatggtc gtggtttcct
      661 tgtatggcat ccaaaaatgg atgagtacat ggaagctctt gacggtcaat tggatgaaat
      721 cagcgaacgc ttgattacac tcggtggaag cccattctct acattgacag agttccttca
      781 aaatagtgaa atcgaagaag aagctggtga ataccgtaat gttgaagaaa gcttggaacg
      841 tgttcttgtt atctaccgtt acttgtcaga acttttccaa aaaggtttgg atgtcactga
      901 tgaagaaggt gacgatgtaa caaacggtat ctttgcaggc gctaaaactg aaacagataa
      961 aacaatttgg atgcttgcag ccgaacttgg acaagcacct ggtttgtaag aaataaggaa
     1021 acatagataa atctgtagga aatttcttac agattttttc tattttgtaa gctattccaa
     1081 accagtcttt ttttgagttt tttgataaaa tagtactatc agtgaaaagg atggaagcat
     1141 gactaagaaa atcgtagcta tttgggccca ggatgaagag ggtgtgattg gtaaggaaaa
     1201 tcgtctgcct tggcatttgc cagcagaatt gcagcacttt aaagaaacaa ctctgaatca
     1261 tgctatcttg atggggcgtg tgacctttga tgggatgggg cgtcgcttgc ttccaaaacg
     1321 ggaaaccctg attttgacgc gtaatccgga agaaaagata gatggggttg ctacttttca
     1381 ggacgtccag tctgttcttg actggtatca ggatcaagaa aagaatctct acattatcgg
     1441 tgggaagcaa atttttcagg cttttgaacc ttaccttgat gaagtgattg tcactcacat
     1501 tcatgctcgg gtggaaggag atacctattt ccctgaagag cttgacttgt ctctttttga
     1561 gacagtttca agcaaatttt acgccaaaga tgagaagaat ccttatgatt ttaccatcca
     1621 ataccgcaag agaaaggaag tctaatggaa cggagtttat ttggtctatt cacggctttt
     1681 ctgtgtttca tctgctttct ggcaggagca caggcttttc gtaaaaagcg ttacgggctt
     1741 tctatcctcc tttggttaaa tgcctttacc aatctggtca atagtattca tgctttttac
     1801 atgaccttat tttagataga atgacagtat agaatggaag gaaatcatgt ctacaaatag
     1861 aaaaaatgat atgatggttt attgctcatt ttgtggcaaa aaccaagaag aagtacaaaa
     1921 aataattgct ggcaacaatg cttttatttg taatgaatgc gtggagttag ctcaggaaat
     1981 cattcgagaa gaattggttg aggaagtctt ggcagacttg tctgaggtgc caaaaccaat
     2041 tgaactcctc catatcttga accactatgt aattggtcaa gatcgtgcca agcgtgcctt
     2101 ggcagtggcg gtttataacc actacaaacg catcaatttc cacgatacac gcgaagagtc
     2161 agaagatgtg gatttgcaga agtcaaacat tttgatgatt ggcccaactg gttcagggaa
     2221 aactttcctt gcccagacct tggctaagag cttgaatgtg ccttttgcta ttgcggatgc
     2281 gacagctctg acggaggctg gttatgtggg tgaggacgtg gaaaatatcc tcctcaaact
     2341 cttgcaggtt gctgacttta acatcgaacg tgcagagcgt ggcattatct atgtggatga
     2401 aattgacaag attgccaaga agagtgagaa tgtgtctatc acacgtgatg tttctggtga
     2461 aggggtgcaa caagcccttc tcaagattat cgagggaact gttgctagcg tgccgcctca
     2521 aggtggacgc aaacatccac aacaagagat gattcaagtg gatacaaaaa atatcctctt
     2581 catcgtgggt ggtgcttttg atggtattga agaaattgtc aaacaacgtc tgggtgaaaa
     2641 agtcatcgga tttggtcaaa ataataaggc gattgacgaa aacagctcat atatgcaaga
     2701 aatcatcgct gaagacattc aaaaatttgg tattatccct gagttgattg gacgcttgcc
     2761 tgtttttgcg gctcttgagc aattgaccgt tgatgacttg gttcgcatct tgaaagagcc
     2821 aagaaatgcc ttggtgaaac aataccaaac tttgctttct tatgatgatg ttgagttgga
     2881 atttgacgac gaagcccttc aagagattgc taataaagca atcgaacgga agacaggggc
     2941 gcgtggactt cgctccatta tcgaagaaac catgctagat gttatgtttg aggtgccgag
     3001 tcaggaaaat gtgaaattgg ttcgcatcac taaagaaact gtcgatggaa cggataaacc
     3061 gatcctagaa acagcctaga ggtgactatg gaacttaata cacacaatgc tgaaatcttg
     3121 ctcagtgcag ctaataagtc ccactatccg caggatgaac tgccagagat tgccctagca
     3181 gggcgttcaa atgttggtaa atccagcttt atcaacacta tgttgaaccg taagaatctc
     3241 gcccgtacat caggaaaacc tggtaaaacc cagctcctga acttttttaa cattgatgac
     3301 aagatgcgct ttgtggatgt gcctggttat ggctatgctc gtgtttctaa aaaggaacgt
     3361 gaaaagtggg ggtgcatgat tgaggagtac ttaacgactc gggaaaatct ccgtgcggtt
     3421 gtcagtctag ttgaccttcg tcatgacccg tcagcagatg atgtgcagat gtacgaattt
     3481 ctcaagtatt atgagattcc agtcatcatt gtggcgacca aggcggacaa gattcctcgt
     3541 ggtaaatgga acaagcatga atcagcaatc aaaaagaaat taaactttga cccgagtgac
     3601 gatttcatcc tcttttcatc tgtcagcaag gcagggatgg atgaggcttg ggatgcaatc
     3661 ttagaaaaat tgtgaggaaa agaaaatggc aaaaacaatt catacagata aggccccaaa
     3721 ggctatcgga ccctatgttc aaggaaaaat cgttggcaac cttttgtttg ctagcggtca
     3781 agttccccta tcccctgaaa ctggggaaat tgtaggagag aatatccaag aacagacaga
     3841 gcaagtcttg aaaaacatcg gtgctatttt ggcagaagca ggaacagact ttgaccatgt
     3901 tgtcaaaaca acttgcttct tgagcgatat gaacgacttt gttcctttta atgaggttta
     3961 ccaaacggcc ttcaaagagg aattcccagc tcgttcagct gtggaggtag ctcgtcttcc
     4021 tcgtgatgta aaagtcgaaa ttgaagtcat cgcagagatt ggataagcta gttgaagttt
     4081 ggtgttgcca aacttctttt gatataagga gaaaaagatg acaaagaaac aatttcactt
     4141 ggtgattgtg acagggatga gtggcgcagg gaaaactgta gccattcagt ccttcgagga
     4201 tctaggttat ttcaccattg ataatatgcc gccagctctc ttgcctaagt ttttgcagct
     4261 ggttgaaatt aaggaagaca atcctaagtt ggccttggta gtggatatgc gtagccgttc
     4321 tttcttttca gagattcaag ctgttttgga tgagttggaa aatcaagatg gtttggattt
     4381 caaaatcctc tttttggatg cggctgataa ggaattggtc gctcgttaca aggaaaccag
     4441 acggagtcac ccactagcag cagacggtcg tattttagat ggaatcaagt tggaacgtga
     4501 actcttggca cctttgaaaa atatgagcca aaatgtggtg gatacgactg aactcactcc
     4561 acgtgagctg cgcaaaaccc ttgcagagca gttttcagac caagaacaag cccagtcttt
     4621 ccgtatcgaa gtcatgtctt tcggatttaa gtatggaatc ccgattgatg cggacttggt
     4681 ctttgatgtc cgtttcttgc caaatcccta ttatctacca gaactgagaa accaaacggg
     4741 tgtggatgaa cctgtttatg attatgtcat gaaccatcct gagtcagaag acttttatca
     4801 acatttattg gccttgattg agccgattct gccaagttac caaaaggaag gtaagtccgt
     4861 tttgaccatt gccatgggat gtacgggtgg acaacaccgt agtgtggcat ttgctaaacg
     4921 cttggcgcag gacttatcca agaattggtc tgttaatgaa gggcatcgcg acaaagaccg
     4981 cagaaaggaa acggtaaacc gttcatgaga aaaccaaaga taacggtgat tggtggaggg
     5041 actggaattc ccgtcattct aaaaagtctg cgggaaaaag atgtggaaat cgcagctatc
     5101 gtgacggtgg cagatgatgg tggttcttca ggtgaactcc gaaaaaatat gcaacagttg
     5161 acaccgccag gtgatcttcg taatgtcctt gtggccatgt cggatatgcc taagttttat
     5221 gagaaggtct ttcagtatcg gttctctgag gatgccggag cctttgctgg ccatccattg
     5281 ggaaatctca tcattgctgg cttgtcagaa atgcagggtt caacctataa tgccatgcag
     5341 ttattgagca aatttttcca tacaacaggg aaaatttatc cttccagtga ccatcctttg
     5401 acccttcatg cagtctttca ggatgggaca gaagtggctg gagagagtca tattgtagac
     5461 catcgaggca taattgacaa tgtctatgtg accaatgccc taaacgatga tacgcctctg
     5521 gccagccgtc gagtagtgca gaccatcctt gaaagtgaca tgattgtcct agggccaggt
     5581 tccctcttta cctctatttt gcccaatatc gtgattaagg aaattgggcg ggctcttttg
     5641 gaaactaagg cagaaattgc ctatgtctgc aatatcatga cccaacgtgg ggagacggaa
     5701 cactttacag atagcgacca cgtggaagtc ttgcatcgtc accttggtcg cccttttatc
     5761 gacactgtct tggtgaatat tgaaaaagtg cctcaggaat acatgaattc caaccgtttt
     5821 gatgaatact tggtgcaagt ggaacacgat tttgtaggtc tttgtaagca agtttcgcgc
     5881 gtgatttcat ctaacttcct tcgtctggaa aatggcggtg ccttccacga tggagatttg
     5941 attgtggacg agttgatgcg cattatacag gtgaaaaaat gagtttcaca gtagcagtaa
     6001 aagaagaaat cctgggccaa caccatctga gctggcatga attatctgcc attatcaaaa
     6061 tgtctggtag catcggtctc tcgacttcgg gcttgacttt gtcagttgtg acagaaaatg
     6121 ccaaactggc ccgtcacctc tatgagtcct ttctccattt ctatgaaatc aaatctgaaa
     6181 ttcgtcacca ccaacggagc aatcttcgca agaatcgcgt ctataccgtt tttacagatg
     6241 aaaaggtgca ggatttgtta agtgatttgc acttggcaga ctctttcttt ggcctggaaa
     6301 caggtattga tgaggcgatt ttatcggatg aggaagcagg tcgtgcctat ctctgtggcg
     6361 ctttcttggc aaatgggagc attcgtgacc ctgagtcagg caagtaccag ttggaaatca
     6421 gttctgttta tctggaccac gcgcaaggaa ttgcctccct tctccaacag tttttactgg
     6481 atgccaaggt gcttgagcgc aagaaggggg ctgtgaccta tctccagcga gcagaagaca
     6541 ttatggactt cttgatagta ataggagcca tgcaggcacg tgatgatttt gagcgggtta
     6601 agattttgcg agaaacccgt aacgacctca atcgggctaa taatgccgag acagctaata
     6661 tcgctcggac agtttctgcc agcatgaaga ccattaacaa tatcagtaaa atcaaagata
     6721 tcatgggctt agaaaatttg ccagtagatt tgcaggaagt ggcgcaactg aggattcagc
     6781 acccagacta ctctatccag cagttagcag atagtctcag cacccctctg accaaaagtg
     6841 gtgtcaatca cagactcaga aaaatcaata agatagcgga tgaattataa cgatataaag
     6901 caaccccttg aattatcaat atcttcaagg ggttttgttg tgttatgaaa tcaaaaaggg
     6961 gcaatcaagg ggcagaatta aaaaatagta tctaatttat tgaccagttt atcctccata
     7021 tcctcagtag tatgagaata gatttccaaa gtcatttttg catttgagtg cccaactcga
     7081 tccattattg attttattgg gaggtcagac tctgctaaaa acgaaatatg agaatgccta
     7141 aaaatatggc tagataagtt tttttctatt ttggcctgtt ttccatattt ttttaatatc
     7201 tgtatgaagc aagctattgt tgtaggttga ttccattttt caaaacagaa aatataatca
     7261 tcgcttgaca atggctggaa acgttcgcta agtcgtacta tttgtctttg aatagcttct
     7321 atgacactct ctgatacttt gattgtccgt attgaatttg tagtctttgg tagcgtcttg
     7381 attttgttta ctgaatcaaa attacctgtg atctcaattt tgttgttttc gaagtctata
     7441 ttcttcagtt gtaaggcagt taactcacca tatctcatac cagttaatgt cagcacaaga
     7501 accatatcag cgtacttttg gtgatattct cgacgattaa ggacatcgac aagtgctttt
     7561 atttcttgca tggtgagaaa gttgttacgc tttttttcca gttcttctaa agtctttggt
     7621 ttttgaggaa tcgtagtata atcgacctcg ttgttttcaa tgtaagagta ttgaacagcg
     7681 taattaaaga tacctctgag cctatgccgt acttttttag ctgtaatata tccgttgctt
     7741 tcaataattt tttcaatagc ctcttgaaga aaacgcctgt caagattagc aagtatggta
     7801 tcggatggta tgacttcctt catcttctta tcaactgatt tacaattatg ttttgttgat
     7861 tcctttactg tttgcgccca tgatttatag aaaaggttat agatttcttc aaatgtaatg
     7921 ctttctactt gttttgtgct gagtttttta tttatcttct cttgtaacaa gatagcagct
     7981 tgatttcttg cctggggagt tttcttctcc atggttactg aaactttttt taatttctca
     8041 gtatatggat ctttatatcg ctcaaaatat ttaaatttgc catttggcaa ttcttccatc
     8101 cacattgcgt ttacctcact tttttgttaa aatgggtaca agaaaactac ctttttaatg
     8161 gttgtttcct atacatgatt tcctcacact ctccttggcc aaaatttgag tgtggggatt
     8221 ttttggttgc tatctcgtca gataataaaa taaggtgtac ctcttagaag tacaccagac
     8281 gattttatcc ttgacgaaca aggctttttt acattcttag tgtatttctt tttttaattt
     8341 ctgtcaagca gttatttcct aaatggaaat agttggtttt aatttatact tttggttcat
     8401 tacggtgcca atcattagga aaacctaaag actggttgat tacattgatg tctatagaag
     8461 gcagcttttt ctctagtttg ataacttttt ttctaagagt attccacaaa atgttgaatg
     8521 ctgctttact aataaagcat tgaaggctaa taattgttga atatacagtt tttctactgt
     8581 catctcccat caagatctct ttgttatgaa tcgtttccca aaacgtacta ttagaacggc
     8641 atctgaagtt taataagcga ttattgtgag cgcatttgtt tcgtacctcg ttaatatttt
     8701 ttagaaaaga tatcaaggtt tctggtggga aaacatcgtt aaagtcaggt atgtttgtgc
     8761 ttataaaact tactaaatcc cgtgcgattt cattttggag tgaattaggc aggttttcaa
     8821 taatcgtgcg taaatctcca aactctagat aatcagttag tacccagatt gggacatctg
     8881 aatgagtatg tgcgtagtgg tgaattgaat ttcctctgta tcgcttgttt gtgtttatga
     8941 ttttggatag ttttgataca ataaatccca cgtctaatat tttattatct gcataagaat
     9001 tggtgtttaa ataagcgtat ttttgactgg ggtaagcttc ggcgaatctg tgagcagtga
     9061 ttgacttgat atggtgttca gcttcaagaa tagcttgcaa aatggctcgt tttacatctt
     9121 tgtcaaatgt atagagactt gaaacttcgt caaatgtgac gccgtcaata taagtgtcag
     9181 taccagggtg ttgaaaaaac ttactgtatc cattgataat attgtagtaa ttgttactta
     9241 gcaggtactt agctgcttta gattcatctg taatggataa ccctctgatt tttagtgttg
     9301 cgatttgttc ctcgattgtt ttaaatggct tcaaaaaaac tcccccattc taatttagaa
     9361 tgaaggagtt tccccgtatc ggtccccgta gagatcccga cgctttttct ctaatgaatt
     9421 cattctaaat gattttttta aaaaagtcaa ctgtttttta atatttttaa aaaataattt
     9481 ttttattccc ctctatacac cccgactact gcataaatct tgatgtgtgt gtcttcggct
     9541 ggtgggaagt ctaggatgat gtcttcgtac ttgtcattga gtgacactag gcgtaagcgt
     9601 ccgttttcgg tatatatctt cttgaagtaa gaacggtctc catatgcgat aattgctaaa
     9661 tctccgttgt aggtagtcag tcctttgtct actaaataga gaatgtctcc gtcttggtag
     9721 tcgggttgca tggagtctcc actgaccttg gtcgcaatat cgtggcgtgg tggtcgctcg
     9781 tcaacctcta tagtctctct gtctgtatcg tcgtacccaa atccatagtt aaatccagaa
     9841 gctgctgccg tctcagatac aacctcaact tggtacaagc tgataacttc cgatacttcg
     9901 tttatcttcg tttcttcttc gttttggctc tccagaagct cctcagaggt ccgtagcacg
     9961 attttttgat tgggtggggt taattgcact accttatcca ttatctgctg tgtgagcgaa
    10021 tctggagcgt ctggcagtga gttggtggat tcttctttaa aagtagtatc tatatctgat
    10081 tttttaacac cgaaataatc agccagtttt tggataacac caaaagaagg agcgcttctt
    10141 aacttcatat agtctgtcat agtacttgct gtaattccaa cttctttagc caactctttt
    10201 tgggtgatgc cacgttgctt tctaaagtgt gtaatatttt cagcaataat ttgcattcgt
    10261 tttttttcgt ccatttacga ttacctcgta ttttttataa ttacattata tatcattttt
    10321 gaatgataaa caatcaaaaa tacgaaaaaa tcgtattata attaaatcaa gcttaaggaa
    10381 aaggaggtaa ggcaaatgaa cgaactagaa agaacagccc tcaatgagat attgaggact
    10441 gtgacatata tagctgagaa gttggatgaa gtagattcta agatttcttt gaacgattca
    10501 caagctcttg agcatcaagg aaattgagtt tcatttccat gtagtgaata actccgtgta
    10561 ggtagttctt taggtctgta aaacctttat caggattatt tctatagtaa tgaccttcgt
    10621 cgttaccgat ataagcagat gcaagcgcaa atgttttaag gtcttcatcc ttgatatatt
    10681 tttcaataac ttgttttaat gacattttaa tgattttatc ttcatcatca ggatttgtaa
    10741 caatggagaa atctttaaca aagaactcaa gcgccttacg gtatccaata cctgcaatgt
    10801 ggtctagttg ttcgtgttca gctttcagtg cttggacata gatttgctta ccaattggag
    10861 aaactatctc tacatcgtca gatataggta tatcgctcgg aagattaggg attactttga
    10921 tatgttcaat ttcgtatttt tcggtatagt cattgattaa aaatcttgta gctataaact
    10981 cctctgtcca aaagtgctta caacctaagc atctacatgt taatactaag cttgttttat
    11041 tttctccaag agaaaaataa gaagagttca caacatctgg attggttggt tttttacaat
    11101 ttgggcaaat gtcttcaacg gttacaggcc tagaaacaga agaatttact ttcactcgaa
    11161 atatcataag atttctccaa tcgttttatt ttgattatac catatttgaa agggggtgag
    11221 gaaatgagac caagacgata tccgtatagt gggaaaaaag agtccacctt cgtaaaggca
    11281 gaccctgagt taatgttgaa tatcaataag attgatgttg gaaatataca agcgaaaaat
    11341 atatttggaa aaatttaagg agtaggagga tggatgaaag aaaatataga acatcttcat
    11401 gaacgcataa agcattttca gtcattgatt gcttatatgt ctgagcatga gaaaaagtat
    11461 tatttagaaa aagattggtt cgataaccca accctaattt ctatagaaga cgcaaaaaaa
    11521 gaagtagaac tactttaaca gaaaggaggt aggaacgtgc agtggacttt agaagctatg
    11581 agaatcaaca aaggacttac tcaagcagag ttggcagaaa aatttgaagt ttcaagtcaa
    11641 acaattgcta gattagaaaa agatagctct gatatcggtt atcagctatt gaaaaaatac
    11701 atgttttttt tcaatgtgaa attcgatgat atttttttag ggaaaaaata cgaaaatttc
    11761 gtaaataact agaaacaaaa aaataagaag gaggaacaca tagatggaaa gcgttcttga
    11821 aagcttgaaa caagaaaaag accacctcga aaaaatcatt aaggtagtta cctctggtgg
    11881 taaatttctg agattgccgt atcaaaaaaa gtcacgctcg attagtgaga atctgaaatt
    11941 gatttctcaa aatcttgata aattgagcga gcaagttcaa caaaccacga atcagcattc
    12001 atgatttcaa gatgacgaaa gaaacctgtt ttgatttcca gtttgatatc atgttacagc
    12061 gactcgataa agcgattagt gattaaattt ttgagatgta agtttttttc atgtcaaact
    12121 ttttcatgtc aaatttttta cactaataaa tattaactaa caacaagtat taaaagacaa
    12181 ccagtcctac ttctctaaat aaaagagagg gtagaaaaaa taaatacaaa ggaggtgagg
    12241 aaatgagacc aagacgatat ccgtatagtg ggaaaaaaga gtccaccttt gtaaaggcag
    12301 accctgagtt agttgaaaaa cttttaagaa acactagttt tcttgagcgt ttacaaaaaa
    12361 agcctatcaa ttttcagata gactcagaag aatttaagcg tcttagctat gaagccattc
    12421 atgatacttc tcaagtaact caatagtagt tattacagaa gtcaaaccac tgaccttccc
    12481 cagttgcaat ccgtctgtat ggtcaatctg tttagtagct tcattagctt tagcagagat
    12541 agcttgcata tcttcagctg ttaaagattc tcgaaaatct ttaaaggatt tcataaaaac
    12601 tcctcctttc tattggaatt ttgactaaaa cggtgagagg tcctagtcga gattattata
    12661 gcaatttagg aggatattac atcggtcttg aggctgattt ttggaggcaa tattggaaga
    12721 taaaatcata gaacttgctg attacttcat cagcgaatct acaacgtaca gagaagcaaa
    12781 aatagcgtgt gagaagctat tgaaacaagt cagccacgag attgaactca gggcgatgga
    12841 aagtaggaca gtatgaaaga agcagtaaag gaatttctaa aattcaggag ccgatttaca
    12901 aaaatagaat ggtttgaaat caaccaagct gtcgaagctc gtttaaatca aaaagccgac
    12961 cagttgaaac tggacgacgt agatttagaa atcatttcta gcagactaga aaaagttatc
    13021 tagaaacgat ttgaatgaac attggatgga tacgatagtc agcgccacga tagtgaatgt
    13081 agatataatc ctgatggtac atcgagtttg cttcaggttt agaaattggt gagtagagtt
    13141 ctgcattttc ttcccaccaa atgtaaggac tagccatatt tggtcccatt acacaatcgt
    13201 cgtcggctga taggttcacc caatttccgc aaagacatgc gtgaatttca gtcataatat
    13261 tacctccttt ctggtttcat tatagcagaa aaggagatag caaaaaagca cctgacggaa
    13321 atcaggcgca tacttaaata ttcaacatga ttataacacg aaaggagcaa aaatggaagc
    13381 agttgaaatt gtaagaatta aagatgtgat tattgaaaaa gtctctgcta atgatgaaga
    13441 gttaaaacgt atctttggat gttcaaaacg acaagcagga gagcgaagaa gagaaatgca
    13501 aaaactccct agtcagcaaa aacatctttt ggatagtgga caacttgtaa cgattaaagg
    13561 tttctatgaa tacttgcaat atcgtggaac taaagcttgg aaaaaagaaa tggaaacaag
    13621 caagaaaatg aggtcagcag gatgaaccta ctatcaagaa tcaaaaacta tttttcggaa
    13681 gaggtcaaag aaactaatct cgactggaaa gaggtcgctt tagacctcaa tcaatcacta
    13741 attgaaacac aggaaaaact tcaagaagcg aatcaagaaa tcgcagactt gaagaaaatc
    13801 gtagcaatct ataaagaaaa ggagaaagaa aaacgatgga atatatttac ctggtaacaa
    13861 tcgtaggaat tggactatgg tcgctagtaa ataaactaga tgaccacgct gaaatgaaac
    13921 aaaaagagcg ccagctgatg gcaaacaatg tcgcacggat gaatctgaga aattcagata
    13981 agcaatttac ttatgatgta gaaccgcctg aagggttgaa ataaggagga gaaacatgac
    14041 tcaagctgaa cgaattaggg aatattatag agagcaccct gctgcctcat atgatgaagt
    14101 ggctgaggtc gttggtacaa caaatagtaa tgtgagagcg aacctggcca aagacatcaa
    14161 ggcaggcaga tgcgttcgct tggaagataa gtcatacgat tactcgcctt actataacca
    14221 tacacaggca ctcactgagt tggttgactg gaagaatgac aacagacgtg agtgggtgga
    14281 tatgctgaca agagcagcag agaaagaaac ggatagcaac gttatgcgtt tgttaatcaa
    14341 agaagcaaat aaattgatga aagaggtgac taagtagatg gtacgaaata aaataggtga
    14401 tttaactaac acgctcttcg ctcaattaga gactctggat gatagggatc ttacagcaga
    14461 tgaattaaaa gtagaattac accgctcgaa acaaatggtc gcaatctcag gtcaaatctt
    14521 acaagcaggt caattggcgc tagatgctga aaaattcaaa gacaaggtag gtgaagtcaa
    14581 tgccccgatc gctttgctgg aaggatgagt acacggagta catgcatgaa atatgccctg
    14641 gtcgtttaac tcctgaagta accaggttgc tgaatgaaaa atttggtacg aattataaca
    14701 agagtcaaat cggtggcgta cgcaaacgtc tagggttagc agttggaaaa gtctatcaag
    14761 gtcgattgct gacaaaggag caacatgatt atcttgtatt gatccaaaaa aataagattt
    14821 ctcgtaatgt cgcaaatgaa atgaaccaaa aatttggctt atcgctaact gagaaacaga
    14881 ttaagagtta tcggagaaat aataatctac atagtggttt gacaggaaga ttcgagaaag
    14941 gtcagactcc tcacaataag gggaagaagt accccaatat gccaaaaaac ggcgggcagt
    15001 tcaaaaaagg taatcgacct ccgaattatg tacctgtcgg tactatcaac tacacaacaa
    15061 acggttatcc aaaagaaaag attggagaac ctaatcaatg ggttttgaaa caccgcaagg
    15121 tttgggagga ccatcacggg ctgataccaa aagggtactc aatcgttttt ctggacggtg
    15181 ataaaacaaa ctatgatatt tcaaatctgg catgtttatc taagaacgaa attgctagaa
    15241 tgaatcaaaa tcatttattc acgtccaacg ctgatttgac taaatcaggt attggactaa
    15301 caaaacttac aaataaaatc agagaggtag aaaaaaatgg ctagtttata cgaactaaca
    15361 ggtcagttcc tgacaattta ccaattggat atcgatgacg aaacaaaagc agacacgctt
    15421 gaggccatcg attggcaaga acaatttgaa cagaaagcag aaggatatgc ccatgttatc
    15481 aagaatctag aagccgacgt ggcaatgtac aaggctgagg aagagagctt caaagccaag
    15541 aaacaggtgg cacagaaaaa gctggattat gtcaaggata acattatggc agctatgaat
    15601 gtcacggggc aaaccgaagt taagagtggt gccctgatta taaaaattgc taagaatcca
    15661 gaatcagtca aggtcaacga agacgacctt ccgaaaaaat attttacaaa aaaagtgacg
    15721 cttgcgccgg acaaaaaaac actcaaagag ttgcttaaat ctggcaagaa agtcaaaggt
    15781 gcggagcttg tccggacaga aaagttggtg attaagtaat ggaattgatg aataaaacac
    15841 gagtaacaga ttcactagca gttgtgattg gaccagaatc gattgaagta cttgttactg
    15901 aaggttttct atttgatgtt gcgattcgtt ttgtaaaagt agacgaaaca aatcttgatc
    15961 aaggaaatga aaagccagta ttcactccgg aatacaagct ggtcacagtt gctaaataca
    16021 aggaaaaacc tatctttgaa tcggaggaag atattcgaaa atttgagaag caagcaaaag
    16081 aagttaaatc gctatttgcc tttgcaaagg taaataaaca aaattggttt aacactgccc
    16141 tttatccagg agtgctgact gagaaagttg gtgtttgatg aaaattttag ctattgatcc
    16201 aagtagtaat aaaattgaaa ccagcacaac aggagttgtc ttgttggata atgcaagatt
    16261 agttgatagc tgggttgtct cttatggtat gagaggtttc gctgattggt ttcacgaaat
    16321 cggaacaaat cttgaattcg atgtagttat tgttgaagaa tttaaggcga gggataacga
    16381 caagtcgaaa gataatagcg tggcagaaac catcgcctat atccaacttt gctatccagg
    16441 tgccattctt caattcaatg caggttacaa gtcggatatt ccaaacgatc ttttgaaaat
    16501 cttagacctt tggaaatttg aaaaaagtca tcatcaagat attcgagcag cagcaagact
    16561 tggattattt tgggcaatga gaaatgatat tgaagaagtg gttcatgata tcggaaaggt
    16621 ggtgagtgag tatcacaata acgctaagaa agtggcaagc tgaagcgatt aaaagaagtg
    16681 aacatttatc taatggaatc tttttagagg ctcttggggg cagaggcaaa actatctgtg
    16741 cacttgctat tgcaaaacat aaaaaagcta aaaaaatcat catcacaaac aatcgactag
    16801 ctattctgaa tggttggata gatgcagtca agtttatgaa ttttgataaa ggtgttgaga
    16861 ttatcattca gacagataga tatcttcaaa atcaagtcaa aaaggggcat aaattagatt
    16921 gtgatgtgct gatagtagac gaatggcaga atatgtcttc tgacaaacaa gtggccttat
    16981 atcgcaaaat aaagcgaaaa tacacgatag gtctttcagc gacaccaatt cggaaaaaag
    17041 gacaaaattt ctatccgctt gaaaaaacgg tatttggttg ggcaacccca aataataaat
    17101 ttgactggca aaagactcat ggaaaaatgg tctatgatcc atttagctat tcaaaagaga
    17161 agtgggaaga ttttcaaaat tatgaaagtt atatctcgag cttgcctaat ttcttccgct
    17221 gggaagagat tgaaggaatt gagaatgcag ttgagaataa cggttttgag attaagtttt
    17281 accaaaagag agtcgcctct ggcaatccag aaaaacttgc agaatttaga aaactaaatc
    17341 ttgtaacagt ggacggcaaa actgcaatgg ccaagcaatc gtttggaaga aagacctttg
    17401 aacgctacct taatcaaaca ggcgtagcag tcgattttcc aaaattaaag ccagtaaatg
    17461 cggatacgcc attgatgtta caacttgacg gtttaatcga acgagcacca cacgatatgt
    17521 tgattgtcag taaatctaag cagattgtca acgtcattag cgagcgccat cctgaaattg
    17581 gaatctggac gggcgatatt caagaaggac tttataagaa attcgtggtt gctactagtc
    17641 aagtgttagg tgtcggagta gacggcttgc aacacaaata ccaaactatt gtcgtattgg
    17701 atccagtaga agaaggttct ggagaatatg atgattatcg acaattgctc tggcgcataa
    17761 caggaagtcg tcagcagcat gatgtaaatg taattgaatt ttattataaa gaaagttaaa
    17821 aaaagaggaa aacaaaatga ataaaacaac tgaaatgatc gtatttcgta gccgtaaaac
    17881 tggagaattt cttaattctt acaaggacag aagttcttta gcatttgcag ctgacttttg
    17941 cagcttggaa tattgtttga agcttcctcg taaaaaatac gaagacaaca aaaagactta
    18001 caaggctctt gctgcagctt ttgactgtga aattgtcgca gttgaagcgg aatacaaatt
    18061 gacctatccg aatggatcag aagttgaacc tatcaagcgt gaccgttcat caattgagga
    18121 catgattaag gatattattg gaggggttct ctaatggcat ttacacttcc agcaaataaa
    18181 ccacaagttc ctaaagatac cccacgaaat tttttcatct acggcgaaac catgagcgga
    18241 aagtcttatc ttgcaaatga attcccaaat ccaatcgttt tgaacacaga cgggaatgca
    18301 gaagctaaca ctgttccaag cattcagctg atcaatgaaa aagatgacaa gggacgaatt
    18361 accaattcag taattaagca gcttggagat atcttgcttg ctctccagac acagaagcac
    18421 tcttatgaaa cagtcgttat tgatgtaatt gacgatgtta ttgaaatgat taagattgca
    18481 gtttgtgatg aattaacccc agttggtaaa cctcgcttga aatccttgtc ggaaattcca
    18541 tacggcaaag gatacgactt ctttaaccaa gctatcacag aattagtcat tgacctcaaa
    18601 gcattgccaa tgaatgttat ttacatcagc cgtcaggtat ctgaatatga tgacaatggc
    18661 aatgccacca aagacaagcc aagcttgaaa gataagtatg tcaatcttat caatggaaac
    18721 tctgatttga tgatccacac tgaaaaactc ggcaacaact acaaccgtga ggttgaccgc
    18781 aagcgtaaga cctattatgc ggaccaggtt gatgacaagg ccatcttgaa aatcttggca
    18841 actatccgtg gggctgttga gcctgcaaag ggcaagctag cccctaaaaa agaagcagct
    18901 aagacaacta aaccagctaa gaccgaaaaa acaaaagagg cacctaagaa agaagttgac
    18961 tctgatgatg aactatttta agaaataaag gagaatacac atgagcttac tagatatcgc
    19021 aaaatcaatc aaaaaagagg gctttgaccc acgcaaagac agcgccaacg gtcctgcacc
    19081 aatcccagct ggtacttatc cagtagtcct gaagaaagca accttcaacg tatcggacaa
    19141 aggctgggaa agccttggtt atcaatttga aatccgtggc ggtgattaca gtggacgctc
    19201 tgaatttgca acatttggca cactgactga atggaacggt aagaaccttg actgggcagt
    19261 tgaacgcact atgaaattct ttatcaaagc cttggtcctt gctggcgaca gtatgcaagg
    19321 aaatgaagaa gacggtaaag ccttggaaga ggctctacaa cgtaaggcag ttggctctta
    19381 ctacaacctt gttatctctg tgactaaggg gaaagatggc cgtgagttcc gaaactatga
    19441 ccttgaagaa gaagaagcac aaccgctgac tgaagctgat atcgatgaag atgacctccc
    19501 tttctaaaaa atagcaagtt ttgggtcatt gatgaaactg atgaaagatt aggaccattc
    19561 aatacatttg aagaggctta tcaatcgttg ttattttatt taaaaatgac tgaagatgaa
    19621 tatcagtcaa actatacggc ccaggaactt gtttatattt acaaagagga gaaaaaacca
    19681 tgccgtcgat gaaagaatac gcattacagt accaaaagtt agggttctca gtcattccaa
    19741 tcaatcctaa aaacaagatg cctttaatcg aatttgctga taaaccagcc atgactccat
    19801 ctgagattga aaacttttgg gacggctacc ctaatgcaaa cattgcccta aagactacca
    19861 acttctttgt cattgatatt gacaaacacg gcaaatcgaa cggttttgaa tcgctaaaaa
    19921 aatggaaaca tctaaattta atcgaaccga cactgcaagc taagacggct agtggcggta
    19981 aacatctatt ctacttcaaa cgagaagatg agccgatcac tcagatgatt ggattcttgc
    20041 ctggtgttga tattaaggct cacgaaaata attatgtgtt agtcgcaccc tctgccacag
    20101 ataaagggca gtatgagtgg gatctggaaa agtctaagga aggtggcacg atggtcactc
    20161 cttcaaaata tttaatccag tctataaaaa aacagtatgg cgaaactcac ggttataagt
    20221 atgatggtaa ggacggtctt agggatttag ttagacgttc acatactaga gaccgaacac
    20281 agactacaga tctctttgaa accatcgccc ttggttttgg tgatgaaggt ggacgaaatg
    20341 acaaactagc aaaattcgta ggtggtctct tatatcgtgc ggtcgacgat ggtgtagttg
    20401 ttcaacttgc aagattagca aatgcaaata gtccaacccc tttgcctgaa aaggaaatga
    20461 tgcgtactat tgaaagtatg attaaaaaag ataggaggtg attgtgattg gtaatgtagt
    20521 aagtattgac tcacaaccta agatgataac gactgccaag ggagacatca aggccaacag
    20581 tccaagtaat gtgttgatgt ctttcaaagc tgatgatcag ttgagtattt acctaaagca
    20641 caacgatttt tcccaagagc atgaactcct taaagatatc aagatcggca acactctttt
    20701 taaaaaaggt gagctccctt ctaactttga ttcagtcgta aaagtttact ttgaaagtgt
    20761 gttaggtgtt gctttctcta gccaggcgat gctggacggc atggaaactt ttttctcaga
    20821 acgttcttac aatccagtca ttgagtatat ggaaaaggct gctgaaaaat gggatggtcg
    20881 taaacgaatt gaccgaatgc ttcaggttta cctgggggct gaagatatcc ctttagtttc
    20941 taaaatcgct caaatgtggc tagttggtgc agttgctaaa gtttatgatc catacgttaa
    21001 gtttgactat gttctggatc tagtcggtgg acaaggagtt gggaaaacat ccctccttca
    21061 aaaattgggt ggcgaatggt atacggatgc cgtaacagat ttctctaata aagataatta
    21121 cgacattatg ttaaagagtc taatcgtcaa cgacgacgaa atggtggcca gtaatcggat
    21181 gagctttgca gaaactaagg cctttatttc taaaactagc ctacgttatc gtaaaccata
    21241 catgaaacgc acagaagagt ttgccaaaaa cttcatttta gctagaacta ctaaccaaac
    21301 agaatacctc aaggacaaaa ccggtgaacg tcgttttcta ccagttatgg cagatagtaa
    21361 acggcaaaaa aaacatccaa tggaaatcga gccagagaca attgaacaaa tctggggcga
    21421 agccgttaca atctatcgtg ctggtgctga tttgatgttt gatgaaaata cagaggatga
    21481 actgaatatc taccgtgaac agttcatgta tcgtgatgaa gttgaattac aagtgcttga
    21541 atatcttgat atgcccgtcc ctgaaaattg gcaaaactgg tctattcagc aacaacatca
    21601 atacacaagt aaatatttcg ataatagtag cgactttgat cctggaagca aaaaactaga
    21661 taaggtctca actcgtgaaa tgatgtacaa cttatttatg agaaattcga atgacaggaa
    21721 gctgtcaacg aagattaaca tgatcatgga taatcatcct gattggaaaa aaagtgtttt
    21781 ccgggcagga ggtaaaagta caaaagggtt cgtaagagtg aaagattcgg aaaaaactaa
    21841 tcggtagcaa ttaaaaaatt atcggtagtc atcggtagca gttgaggggg agatcggtag
    21901 cattctaccg ataaaatagg acatcggtag cacatcggta gcagtctaac cccttgatat
    21961 tactgacttt tatttaatat ttatatataa tgctactctt ctacctatat ttttaaaaaa
    22021 agtatataaa ataatagtaa taataaagaa agcctataga atagggattc ttgaaaaaaa
    22081 ctttttactt tttagaattt atcggtagca cggtagcagt ttagaaaaaa gaggtaaaaa
    22141 tgtcatacac agtaacatta ttttttgaca acatggtaga cgaaactcac ttttttaaga
    22201 aagagagtga tgctgccaaa tgcaaggctc agttagaaag caagtatcga gggaatcgaa
    22261 tgtataaagt taagcaggag aaattggaag aatgaataag caggaactga ttaaacgtat
    22321 cgaggatttg ccttatacag aggggcctat cgcagataca atcgaaatta atagaaattg
    22381 gatattgaaa tcaattgaac agctagccga atccgaaata ggtcacgcag atgaagctcc
    22441 acgctacgta aagaacatac tagcacgatt gcgagaattg ccattgcatg atagagagtt
    22501 ttggttaaag gctatcatga gcgaatttga acaggatttt agccatgcaa aatggcgaga
    22561 gggctacgag caaggtaaaa ttgagggtat ggttgaacgt gaaaaagtca tagttccgca
    22621 gtgtgtggcg gaatatatag aatttaaaaa gaaaaacaat tttcatgttt acggtgcaat
    22681 gagagtaatt gaagatcatt atgataagaa agttcctgat tggttttacg aaaataacat
    22741 cgaaaaattc tgtcttgctt ggcttgacgg ctacgaggtt gaaaaagaga agcggtattt
    22801 tgttaagatt aaagggaata ttaaagaaaa tatgttggtt tatggagaac ttttgaaaag
    22861 gtatttcttt acaaaaagct ttagtttaga cgatgttata tattcccaca cccgtaaaga
    22921 actagaaaat gcaaaaatcg gctgggtgtt tgattgtgaa gggtttgaga ttgaggaggt
    22981 ggagtgatga gccttacgct aaatagcaca attggagact tagttttggc aatcggagaa
    23041 attatcgttg gttctgatgg taaaaccact acagcgatac tggagatacc tgatcaaagc
    23101 ttttacttag agattgagct taaattgaag gaggaggtca taaattgaaa cgattcatag
    23161 ctatctggat tctgctatct gctggattga atatctggca gagtatctac attaaaaagt
    23221 tagaagaaaa gcgccctatt gtcgtctata aagctgataa cgcaggcgct gagatattcg
    23281 gtaaagtcgt cgagaaagga cggcatggca agctatacac gcttaccatt cgtgactacg
    23341 gggtgttcgt ggttacgaag gacgtgtatg agaaagtgaa agtaggggat gaggtgttac
    23401 tctaatggat gatattttac aagctttagc aaaaatgcta aatatgactg ttgatgaagt
    23461 aagttctttg cttacaacat ttaaagggaa tgcaccacag atttatgaaa tgttcgttaa
    23521 agaaaagatg ttttatgatc tcttcagtct ttttcaaatc atgtcaattg taatatttag
    23581 tatttctgca gtagttttag cagttttaac tctcatatat tttacatacg atggtggttt
    23641 tgtttattcc tatgatatac gtacaggaaa aaccgaggaa gaaattaaat tagaacgcat
    23701 tgaacggaaa agaaaggact taaaaatacc actaaaaatt agttgcattt catcaagcgc
    23761 aagtttgata acattagtta ttgcaattgt tttaaaagca actcttgcac ctaattatat
    23821 attcatcgtg aatgagattt taccaaaatt aacgaagaga taggagttat catgaacgca
    23881 ctagaaaaag tcgaacaatg gtttatcgac cgtgaaatca aagaccgaaa aggtcgctgg
    23941 attgatggct cgtttgtcaa agaggaggat ttggcatgat accgaaattt agagtgtggg
    24001 taaaaatagg aaaacgtatg gttttttcag atgacattct tgctattgac tacgaaaaca
    24061 aagaaatagt gacacaacaa gtttattttg agaatggttt accagacgat agagatatct
    24121 attgttatga ttttgacgaa atcgaactca tgcaatcaac aggactcaaa gacaagaacg
    24181 gcaaggaaat ctttgaaggc gatattgtac gaactactag atttttgggt agagctgacg
    24241 aaattggcgg tttctatgag tatgacaagg aatttatagg gattgttaag cagcttgagg
    24301 gttcttgggt aattgatacg ggcagtgacg cagtatgttt atggactgaa attgaagaaa
    24361 atgaaatcat cggcaacatc tatgaaaata aggagtttgg aggacgcaaa tgagaccttg
    24421 taaatatcca tattcaggaa gaagaaaaaa acaagaaacg ccgtcgccaa tattttctgc
    24481 acgaccaatt tttgacgaag ttccaattgt agaagaagtt aaggttgagt tcggagttga
    24541 agctagtatg gggcgcatat atccagaaac gttaatacat ttagatattt ctggatacgg
    24601 aaatagagtg cattcagtac atcgcttccc cggtatttta ctgagtgttg gtgagtcaat
    24661 ccaactaaag atgcttttct ataaaagact tagaaatttt actacagatc gtttcttgac
    24721 gtttagagaa tctgattgga agttctttat ccgggacctg gtcaacgaat ttaagcatta
    24781 aaaaaagcca agacactctc tgcctcagct aatagttctc gcaaagacta ttatatcaca
    24841 aaggagacag agagtgaaca aggctaaaga gctcttgaaa gagttgcagg atctggacat
    24901 ggacatccaa agccgtatag acgaaattaa cgagcttgag gcaggtttgc tctcaagccc
    24961 taaatggtcc gaggttaaag tccaaggtgg acagactaga aaagttgatg atgtctatac
    25021 tcagttggta gtgatgaaag aggctataga gcaggatact aaagaggtca ttaacagaaa
    25081 acttgaatta ggtagaatga tcaataggct taaaaatcca aagtacaggg cagtattaag
    25141 aatgacttac atcaacaaag gcaccgctga tagcgtttgt tatgatttga atatgagtcg
    25201 tacaacctac tacaggttaa aaaatgaggc ggtcttagct ttggaagaag ttatctaacc
    25261 tcatagtgag cttatgggac tttttggaac agcacggttc taaaaatctg ttagaatggt
    25321 agtatcaaga attgaaaaga gaggtctcag aattggtaga tggttacctg taatgtcagg
    25381 gggctgtaat ggccttggag gttcaagtcc tcccctctcc tttgagtgtt tgtgtcccag
    25441 aatgagttaa atcttctggg tggggattca catatcactc attaactcct atcactcatt
    25501 aacttaaaaa tggttgcgga agcgactgga cctcgcatga ttgcgtagct aattatattc
    25561 cggataagtt ataagctaga gggtttgatt ccctcagagg ttttaaagac tacaaaaaat
    25621 aaaaaagaag tcaaaattta atacgcacgc aaggtagtag tcgccttgca agaaggtcgc
    25681 acatcgtgtg gctttttttg attattcgaa gggtggtgat ggaaaattga gtggattgag
    25741 aataaaacaa aagagatttg cagatgagta catcatctca ggtaatgcga cggaagccta
    25801 taagaaagca ggttatcgtg tttctagtga tagagtggca ggcgttgaag gacataagtt
    25861 actaaagaat cctaagatta aaagctatat agatgaacga ctgaaacagc ttgattctga
    25921 aaagattgcg gatcagcaag aggtcttagg ttatctaact tcagtcatgc gaggagagac
    25981 gcaagaacag accttgataa gcattggaga actagggcag acgattacgg atatagatgt
    26041 aggagctaaa gatagaatca aggcggctga acttcttggt aaacggcata ggctttggac
    26101 ggataaggta gaggcggatg tttctggaac ggtggtgttt gcaaatgagt cagacatatc
    26161 agattaaaca aaacgatatt gtcgttgact tacctaagac agtaggagct ggatacggac
    26221 agttctggcg ctcaagaagt ctttatcgtg tagtcaaagg gtcccgtggt tcgaagaagt
    26281 ccaagacaac cgctttgaat tatgttatcc gtcttttgaa gtatccctgg gccaacttgc
    26341 ttgttattcg tagatactcg aataccaaca agcaatcaac ttatacggat tttaaatggg
    26401 cgtgtaatgt gttgggtgtg actcatttgt ttaaatttaa cgagtctttg cctgaaataa
    26461 ccataaaagc gactggtcaa aaaatcctat tccgtggttt ggatgatgaa ctcaaaatta
    26521 catctattac agtcgatatc ggtagtctgt gttgggcatg gttcgaggaa gcatatcaaa
    26581 ttgagactga agacaagttc agcacggttg ttgagtctat ccgtggtagc ttagatgtac
    26641 ctgatttctt taaacaaatc acagtcacat ttaacccgtg gaatgagagg cattggctca
    26701 agcgtgtatt cttcgatgaa gagacgagac gggctgacac attcgctact acaaccactt
    26761 ataaatgcaa tgagtggctt gatgaagtcg atatcaaacg ctatgaggat ttgtatcata
    26821 cgaaccccag acgtgctaga atcgtttgtg atggcgaatg gggagttgct gaaggtttaa
    26881 tctacgaaaa cgtgactgtc aaggatttca ataaagatga attactacaa gattcagcta
    26941 ataaattatg tatcggtctt gactttggtt ttactcatga tccaactgct ttgtgttgtt
    27001 cgttgataaa tgacacgacg aaagagattt atgtctttga tgaggcgtat aaagtcggat
    27061 tgataaccaa agaagttgcg aagatgataa aagacaaagg ttatcatcgc tcacaaatca
    27121 ttgctgatag cgcagagtca cggctgattg aagagctcag gtcagaacat ggcatatcta
    27181 gaataaaaga gagtcggaaa ggtaaggata gtattatggc aggcgtatcc aaattgcaag
    27241 gatacgctat ttatgtgcat ccagattgta aaaacatcat ggatgaattt tatagttact
    27301 gctaccagcg agataaagaa ggcaactggt tgaataaacc agaggataaa aacaaccact
    27361 tgatggacgc tttgcgttac agccttcaat gtatcgaagg tggaaaagca accgtccgca
    27421 gacgttctga ttatggtcta tagagaggaa agacatgtac caatatttaa cctatccacg
    27481 ggatggatat gatgagggtt ctttgaagaa agacctgatt tacaaattga taacgataca
    27541 taacactgaa agctcacgct tgaagaattt aaaaagctac tacatgggtg atcatgctat
    27601 tttaaatcac aagagacgca acgtgaacgc acccaattac aagacggtag ctaatcatgc
    27661 caaggatatc gcagacacgg ctacgggcta ttttatgggc aatcctatca agtataacaa
    27721 tactgctgac ggtgatattg atgaactact tacagccttt gatggtgctg agattgacca
    27781 agtggatgcg cagaacgcac taaatatggc tatctacggc cgtgcttacg agtacatcta
    27841 tgctaaagag ggtatgactg agttggattc aactagtatt gatccggaga atactttcat
    27901 ggtctacgat gatagtattg agcggaagcc tttgtttgcg gtctattact atgaagtaaa
    27961 agacgatacg aaagacacta ccaagcacca ggctgaggtc tttaccgaaa atctgcacta
    28021 tcacatggtg ctgagaagta cagattcagg aacaactcag agcgaggagg caacacctca
    28081 caaccttggt caaatcccaa ttatcgagta tcgcaataat cactttgcga ttggcgacta
    28141 cgagcaacaa attagcttga tagacgctta taattccttg atggggaatc gtgtcaatga
    28201 taaggaacag gctgtagagt ctatccttgt cttgtatggc acgcagttag cagacactcc
    28261 agaagacgct aaggtagcaa tgaagattct ttctgaagaa ggtcttttgg aattgccggg
    28321 cgatagtgca agggctgagt tcttgaagaa tacgctggac gaaagtgcta ctgaaatctt
    28381 gcgtacagct cttaaagagg acatctacac atttagccat gtgcctaatt tgactgatga
    28441 gaatttcgca gggaatacat caggcgtagc catggaattt aagctgatgg gccttgagat
    28501 gattactaag accaaggaag cgaactataa gcgaggattg cgtcagcgta ttgcgatttt
    28561 tgctcattac ttaggcatga agcagattgc tttagagtct cattcaatcg ttccacaatt
    28621 cagtcgtggt ttgcctaaga acttattaga aatctctcag attgtgaaca atttggaagg
    28681 caaagtgacc aatagacagc ttatttctct cttgccgttt gtggaagacc ctgacgctga
    28741 gctggaagcc ttggaagaag agaaaaagaa gaacatggaa gacatgccga tgttcaacaa
    28801 agacaacacg aaacccgaag acgaggtaga ggatgaagaa tcaggagtat tgggcgaaga
    28861 ggaaagccaa tctgatttac cagcagatgg acaaggccga aaagcaggca gaccagttcg
    28921 ataaggtcta tcaggaagct aagacttact tggataagga aatcaataag attttcgata
    28981 aattccaacg tgattatggt ctaagtcagg tagaagctag acaagtcttg aagaacatga
    29041 aagacaagaa aaatctgaat gaacttcgta aagtacttga agcgagaccg aatgacccga
    29101 acatccaaag attactagct gacttagaca gcccagctta ttctttccgc atgaagcgcc
    29161 tagagcgttt gagcgacgat ttagatcgta tgcgtgaatc tatctatcat tcggagaaga
    29221 caggctcaga tgccttttat agcgacctga tgaaggatag ttactacaag gctacctttg
    29281 acctacaaca gcagacagga ctagcatatg gcttttctgg gcttcctgag aacgagatta
    29341 aacatctaca gtcttttagt tgggtaggag atggaagtac gtactcaaca gacatctgga
    29401 agaatacagg aaaacttact tccagcataa aagatgaact cctcattagc ctcatgacag
    29461 gcagagatac acgagaaact gcacaagcaa ttgctgagag gttcaatgta ggtcagaacg
    29521 atgcaaggcg tttggttcga acagaatccg ccttttttca taaccagatg gagctactca
    29581 gctatgaaga agcagacata gaaaagtata tctttgtggc cgtcttagac aagcgtacat
    29641 cacgcatttg tcaggagcat gacaatcagg tctatgatag ggataaggct gtccctggtg
    29701 tcaattgtcc gcctatgcac ccttggtgta ggtctactac tgtcggatac gatgaggacg
    29761 cagactacag caagttgaag cgcagagcaa ggaatccaga gacaggtaaa gttgagtacg
    29821 tgcctgccga tatgacttat aaagagtggt atagcaagta tgtggatggt aatagagagt
    29881 ctattaaacg taaagcgttt gataaaacta ttaaagatgg tataatagta agtgtatcag
    29941 ggactacaat tggacacact ccgcctggca aaataggttt gcctaatagt gtagttcagc
    30001 ataatgctac aaacggagat gtccttggta gaacttacta tgatgctaga ggttttaaaa
    30061 cgaaagatgt tcattttaca aaccataaac aaccggcacg tcatccttat ggaaaaatcg
    30121 gagaacatgc tcatgatttt gtatttgatg atgaaggtaa gttcgttagt aggagtacta
    30181 gggaattaac agacgatgaa agaaaggaga atcaagatat attatggcga tattagatga
    30241 tttacaagcg ttatatgata atggatggga cgcttctttt aattataatg gtcaagtatg
    30301 tggcattttc cctaattcta tttatgatgt tgttgttatt attgcggacg acgaatatag
    30361 agcatcttct tttgacgatt tgatttcttt acagattgaa gggaaaactt taccggaaat
    30421 catgaacgaa gttgaagtac aatatggcta aagcacctag agagatctaa gtgctttttt
    30481 agtacccaga aaggagacta tccgtacgat tttcttgatt gtgaatttga agtaaaaaga
    30541 atttcagtga tagcttgcga tactatcctt atagaagcct aaaagaaagg aacagaaaaa
    30601 tggaagaatg gaaagaaaga tttaaaaaag aatactacga attgaaagaa cgattccaga
    30661 agttagatat gatgattggg aaatacgaaa aagggcaact agagtttgaa tctaaatgtc
    30721 cgattgattc gttaaaaggt cagcgttcaa ccatgtggaa ttatttaaga attctagaac
    30781 aacgtgcaaa aattgaagaa attaaactat aaaaattaac cgcatcgaaa tcgatgcggt
    30841 tttcctatgc cctaaccgta tggaatcccg tacggttttt atattgtcca aactgtaccg
    30901 atgacaataa aagctgtgct gttccgtcgc cggacgtaaa acgagattat cgagtggcga
    30961 cgtaatcgct ggaggacaat tatgtcagaa gaaatcaatg caactgtatc tactgaatca
    31021 actgagactg tcgacactca aggaaatgtt gattcagtgc aggaagaaaa gcacgaacga
    31081 actttcactc gtgctgaaat cggtaagatg ctatctgccg aacgctctaa atgggaagct
    31141 gagcaagaag ccaaggaaaa cgaagctaag aaacttgcca agatgaacgc tgacgagaaa
    31201 caaaaatatc agttggatca gcgtgagcaa gaactagctg accgtgagaa ggctattgct
    31261 cgcaaggaat tgaccgcaga agctaaagca atgctaagtg aacgtgactt acctgttgag
    31321 ttagtaaatg tagttgattt gacaagcgca gagacggtat cgcagtctgt cgctgtattg
    31381 cagaaatcat gggagcaagc cgtgcaaaaa ggcgttcaag aaaaactaaa aggcggagct
    31441 ccaatgaaac aagcgccagt cgatagtgac ggtatcacaa aagaagaatt tgctcgtatg
    31501 ggttatcaga gtcgaaatga gctctatcaa aagaacccag agctttataa gaaattgaaa
    31561 ggataataga aaatgacagc aggacaaact aaattagcca ctatggttaa tccagaagta
    31621 atggcggata tggtagccgc taaattacct aaattgatta aatttacacc gctagcgtat
    31681 gtagagacaa agcttgaagg tcaaccaggt agcactttaa cagtgccagc atgggagtat
    31741 gcaggagacg ctactgaaat tgaagaaggc caagcaatta cgccagacca attgactact
    31801 aaaaagacta ctatgaccat caaaaaagca ggtaaaggtt atgaaattac cgatgagtct
    31861 cttttgtcag gtcttggtga cccactaggt caagcgactt accagcttgg tttagctatt
    31921 gccaacaaga tcgataatga tttggtagcg gtagctaaaa ctgcaacaca acatattaca
    31981 gaaactccaa caactcttga ggcaatcgat aaagctctag atatctttga ggacgaagaa
    32041 gatgcacagt atgttgctat catcaaccct aaagatgcta ctaagctaaa aactgcagta
    32101 gcaaaagaat ggattaaagg ttcagagctt ggagcaaata tggttgtttc tggaaccttc
    32161 ggtgaagttg atggtgtgca aatcgtgcgc tctaaaaaag ttgatgaagg taaaggcttc
    32221 cttgttaaag tgtcaccaag tcaaactcag acagacgacg ctaacaaata tggagctttt
    32281 gttatcttgc ttaaacgtga tgtggctatc gaaacagacc gcgatatctt gaagaagact
    32341 accgtaatca caggtgatga acactacggc gtttaccttt acgaccctac acgagttgta
    32401 aaattcggtg gcgcgtaaga ggtgacgata tgagtttatt gctacgacgt cattatattc
    32461 aagaagagca ggttaaccag tattctgatt tagagaataa aactctagaa gagttgaagg
    32521 atctagcgaa agaagcaggt gtagcaggcg cttataagtt gacaaaagcc gaaattgtag
    32581 aagttttgga ggaactaaaa agtgaaattt aaaatcaaac aagatttcta tgattgggaa
    32641 tcaaatgtga aacgactggc aggagaggaa cttgagatta ctgaggagcg ctatgctgag
    32701 ctggctgaca atattgccag caacggtgtc actatctcag acgttcttga gaaaatcctc
    32761 cctgaacctg agttcttaga agaggattga tatgtctata gagttgctga agaaattaac
    32821 aggcgaagaa gatactcagc ttctcatgtt gctccaaacg agggctacaa atcttatctt
    32881 gtcagagact aatcgcacat ctttgacacc tgctttaagt cttttaatac ctgaggttgc
    32941 tatcgaactc cacaaccgct caggagcgga aggagagcat tctagaaccg agggtggtat
    33001 agcagtagtc tacggagaaa acggcctgtc tacggatctt ctacagcgaa tacgcatgca
    33061 caggctagca agggtggcag gtcatgtttt tgaagcagag tagactgaaa ccttatccaa
    33121 tgcgacggtt tgaaaagact gtcactgagg aaggtgtcgc gaaagaaggg tatgccaagg
    33181 aagctgagac agtccgcctt gaattgtggc cagctagtag caagctacaa tctgaattgt
    33241 atggcgagcg tgtcaatgat attttgaacg caaatgccaa caagtcagct actatcaaag
    33301 taaaagatgg tgtgtgtatc gatagtcaga cggaagtgac tcatagggtt atttctaaaa
    33361 aggtctacac acatcatcaa gttttggagt tagagcgtgt cagggctact aggggcagat
    33421 aggcttatag ctaaatgtag acgattggct agtaaaaaaa ctggcgagga tatcgtctta
    33481 cgtgcggtgc acaatgctac tataaaggtt gtccaagcag atgcaagaag actcgcacca
    33541 gcgagagatg gagagcttat aactagtatc aaaactaggg caaaaatgga cggagataag
    33601 gctataggcg aagtttacac caaccttaaa tacgctcctt atgttgagtt tggaacggga
    33661 ccaataggac aagctagcca ttctggtatt tctccagagg tcagcgtgac ttacaagtct
    33721 agtccgtggt atgtacatga agaccaaatc aatgtaggac cgtaccactt tcaaaaaatc
    33781 ggggagttct acaagatgta tggtcaacct gcccagcctt atctttatcc agctttgaga
    33841 gacaatcaag agcgtgtgtc taagaatatt tcgaattatg tccgtagaaa gataagagaa
    33901 caaataaaat gattaatatc aagcctgtta tttataaaga attgcaaaag gttgcagata
    33961 atgtgaccga tacttatcct agcgattggg agactttccc agtcgttatt tttttggaag
    34021 aacaaaacaa gccgggtgat tggtttgatg accaggaaca aaaatcctct atccgctaca
    34081 aggtggatat ctttgatgat actagcacta gtgagttagc tgttaaaatc aatcagattt
    34141 ttgagtcttt aggtttgcga agaactgact gccaagacgt gccagacccg tctcatttga
    34201 gacataaggt catgcgtttt gaaggtatcg tcgacttaca ctcagagctt gtttttcaat
    34261 ttagaatgga gaattaaaca tgttagcaaa tggaattacg ctttcttatg gcgaaacaaa
    34321 agatagctat actaaacttg ttggattgaa agaagtacca gagtttggta ttgaactcga
    34381 aaaagtagaa aatactactc ttgaagatac ggtgaagaag tacgagtttg gtattgggga
    34441 cataggagaa cttgagtaca agttctctta taataattca agcgcaactg ctccttatcg
    34501 tgtattgcgt aaggcagcag acgacaagaa gaaactctac tttgaacaag cttatccaga
    34561 cggtactaag gtcatttttg aaggccaagt atctgttaag cttggcggtg gcggtgtcaa
    34621 tgccgttatc gatttcacac ttaagattgc cttgcagtct aatcttacat ttactgatgg
    34681 tattggaggt taattaaatg gcgttaaaat acacaacttg gaaagttact gacgaaaaag
    34741 agttgaagct acgtttgaca tctcatcaag ctgcaactgt ggaagaaaaa atcggcatga
    34801 acttgctgaa gattttcatg cctgaagctg gcgaagagtt cactttaccg cctttgaaag
    34861 ttatgttgtt gttagttcac ggagccttgc agcagtatga acatgggtat tctcttgagg
    34921 atgtctatga tttatacgat gaatacgtgg acaatggcgg agaccaaaca accttcatga
    34981 cagaggtgtt gatgccactc tttgaagtat cgggttttac tccacgagga agcaaggaca
    35041 agaaaacttc caagaagaaa atgacagtag acaagtaatc ttaacggtaa ctcagattat
    35101 tgagaggctt tatcctatgt ttttggacat cgagggcaag cctcttgatt tttgggattt
    35161 gacggtactt gaaatcagag aaatgattga aagctataac cgtgtcaaaa tccaagagcg
    35221 taaagaaaag attattgact catacagact ttcgcagatg atatccaacc acgtttcttt
    35281 attgttatcc aaagatgcca aggcctttga gttctgggaa tatgcgcctg agttgtttgt
    35341 agaagaacaa caagcagtag aacaggaacg acagaaacaa gcacttttgt tgcataagga
    35401 acggatgcgt gaatttgcag agagacataa tcgaaaaagg aaggaggaag taaatggcaa
    35461 ctcttgatga attgaaagtc atgattgacg ctgagatagc gcctttcagg aagaagatga
    35521 aagaagtcga gaatcaggtc aaaggaacat ctgaccaagt gaaaaatgct actgccaaag
    35581 ttcgtgaaca gtcgagctca atcggtagtg cgtttggcaa gctggctaag ttcgctggtt
    35641 ttgcaatcct tggtaagaaa ttacttgatg ttgggatgta ttcaacgcag acggctcttg
    35701 aagtatcagc gtctatgaac caaatcaagc gacagatggg cgagagttcg caatctttct
    35761 taaaatgggt taacgataat gccaacgcta tgaatatggg tgtgggtgag gctaccaact
    35821 acggtgcagt ctactcaaac ttattttctg gatttatcaa agataccaac aagctaagcg
    35881 cctataccgc taagatgttg cagacatcgg cagttgttgc tgaaggttca gggcgcacga
    35941 ttacagacgt tatggagcgg attcgctcag gtttactagg gaacaccgaa gcgattgagg
    36001 acctaggaat caacgtcaac gtggctatga ttgagtccac tgaagccttt aagaagttcg
    36061 caaacggaca gagctggcaa cagttggatt accaaaccca gcaacaaatc cgccttatgg
    36121 ctattctgga acaggctaca gccaagtatg gggatacctt gtctaattct gtaaatggtc
    36181 gtatcagcct atttaagtcg ctgatgaagg acgcagcatt gaaccttggt aactctatgt
    36241 taccgattat caatgccatt atgcctgtct tgaactcttt tgctatggtc ttaaagaacg
    36301 ttactgctaa actcgctgag tttatcgctt tgatgttcaa caagaaagca acagtgaaag
    36361 atggtgttgg tggagcagtt ggagacatgg gtaacgccat gaaggatgct gcaggcggag
    36421 caggagacct tgctgacgca gtagacgacg ctggagattc agcaggagga cttgctgaca
    36481 atcttggaga ctccgccaaa aacgctaaga aggccgctaa agagttgcta ggtcttttgg
    36541 gatttgatga gattaacatc ttgcaaaaac caaaagatga cgacgcaggc gggtctggag
    36601 gcggtggcaa aggtggtaaa ggaaagggag gcggtggcgg acctttcaaa gacatcttgc
    36661 cagaagtcga gttgaccgac atggacaaca aattcaagag catttttgat ggtcttggag
    36721 ataagctcaa agggttgttt gacctcttca agaaaggttt tgatgcagca tttagaccag
    36781 aaggtataga acgcattaag actgccttag accaaatagc taagacaatg ggagaaatcg
    36841 ccactgaccc aagggttgtg aatgccttta accgaatggc tgagaaaatt gcttatgctt
    36901 tagggcaagt gacaggctca ataacaacta tcgggctagg tatcggtgtt ttccttgccg
    36961 aaagtattgc aaatggcctt ggaaggcaaa aagaacgcat taccagggcg ctagtcgctt
    37021 tgtttgataa tattggtaac atttccgagg cagtaggaaa catcgctcag gacttttcta
    37081 gtactttcta cgacgtcatt acctcaactg gtgcggttcg tatcggtagc gctattgtgt
    37141 caactctgtt gagtttgaca tctaccattg ttgaagttgg tagtaaatta gcaggaagtt
    37201 tgtttaaagg ttttgaaaaa gtcgttgtga caagcgctcc taaaatttca tcagtcttcc
    37261 aaagtttatt agatactgtt gcgcctgtat ttgagagcat tgaaaggtct gttaacaaat
    37321 ttggcgatgg cttaagtcgt gtttatgatg aacatgtagc ccctgctatt aactctattg
    37381 ctaatgcttt taacggactg attgatatca tacaaatcct ctgggagaat tcttggcaac
    37441 cttttgctga gtttttatca ggagtattcg gtgttagtat tgaaggaatt tcagatttat
    37501 taggaggtgg ccttttagcc actttgggac tattggcgga tgctattaag ttagtggcag
    37561 atggtttcac cgttttttct gactggtgta aagaaaacaa agaacctatc ttggctttga
    37621 taacaacttg gcaaacgatt aatttcttat catgggctga acaagccggg ggacttgcag
    37681 gagcattcag cttgttaggt agtaaggtct ctttgattgt tggagggatt aagaatctag
    37741 gtcttgctat taaagcattg acatttgata agttggtcag ttttggtgaa acaatctatt
    37801 tgaacacctt atatgcaaaa gattttgtgg tcaattcagg taaaacaatt gcacagctag
    37861 gaaaaactgc tttagaactt ggtaaatcag ctctagcatg gactgctcat gcagcgaaaa
    37921 tgggattagc aaccgcggcg gaatttgcac attctgttgc agcaggagtc gctacagctg
    37981 caacatgggc ttttaatgca gcgttagcag ttttgacaag tccaataaca tggattattg
    38041 cagcaatcgc agctttgatt gctatcggtg tcttgctcta ccaaaactgg gacactgttg
    38101 ttgagtttgc taaaactgca tggcaaggac tatgtgattt tattagtggt atttgtcgag
    38161 ctattggcga atttttcagt ggtctatgga caaaactaca agaaatcttt gagccgatag
    38221 gtcaatggtt tggcgagaag ttccagcaag catgggatgc cattgtcaac atcttctctg
    38281 gtatcggaga gtggttctct ggtgtattcc aaggtgcatg ggacgctatc gttaatatct
    38341 tcactccaat cggctcatgg tttggacaac gttgggcaga tgtgactagt gctttggcta
    38401 atatcggggc atggtttact gacatattcc aaaaagcatg gactggtcta acaaacatct
    38461 ttagcaaact aggtttatgg tttggcgaga gatgggcaga tgttacaagt gttcttgcga
    38521 atgtatcttc ttggtttggg aatatgttta ctagtgctta taatgcagtc aagaacgcgt
    38581 ttagttcaat tggtggcttc ttcagcggtg tatggtcaac ggttcaaagc atatttgtca
    38641 atgctggaca aaaggttggt agcgctgtag gtggagcgtt taagagtgcg gtcaatgcgg
    38701 ttcttggaac gattgaaaat gtagtcaatg acttcatcgg aatgattaat ggagttttag
    38761 gcgttgtcag aaacttacct ggtctaggat gggttggtag tgtaagcaca gttagcctcc
    38821 ctcgtcttgc ccgtggtggt atcgtcgata gtccaacaat cgccatgatt ggtgaagctg
    38881 gtaaagaggc ggtcgtacca cttgaaaata caggatttat ccaaacactt ggacgagtag
    38941 tcagcagtgc ggtagtaaat gccatggctg gtattagtcc acaaggtggt ttttctagcg
    39001 acggcgacat cgttattcaa atcgcaggcc atgagttcgg acgggtagct atccaagaaa
    39061 tcaacaagga acatgaacga gcaggtcaaa ccttgctcaa gatttaggag gttaaatggc
    39121 acaattgaca atcaatgggg tggctgtgaa gcctcccaaa tcttttcaag tcggtattca
    39181 agatatcgat ggagagacag ggcgtaatgc caatggcgac atggtgcgtg accgtatcac
    39241 gaccaaacgc aaactagact gtgaatgggg tatgatgact cagggagaaa taagtcagct
    39301 tttacatgct gtatcatcta aattttttga ggtatcttat ccagacccca tggatggcca
    39361 agtcacaaag actttctatg tcggtgatag gacagctcct agctatacct ttactgagaa
    39421 gcttaaacct tggtctggcg ctaaatttaa tctggtagag aggtaagaaa atggacgctt
    39481 taactagacg acaatttgac agagccatgt ttgccaagga aaggacgctg gctattcgtg
    39541 ttggtgaata tgcttcacgg gatatcaaag aggctagttt tgagtatggc tacatcaagg
    39601 gcgatactta taagcctggt ggaacctgcg ctggtagcgg taaaattacc tttaccagta
    39661 tcattaccac gttcaataag ctggataccc tgcaccctga gattggtcta ctggttgggg
    39721 atacctacca gtgggtcaag atgggggaat acttcatcaa cgatattgag attgaccgaa
    39781 accgcaacac aaccacactt gaacttatgg acggtatgtt taagctcaat cgtgagtacg
    39841 tgacggattt gcatttccca gctgaagtac gagaggttat tcaggaaatc tgcctgaaaa
    39901 caggcattga gttagcgaat gactatttcg gaatcagcgc tatgcgttac catatcgagc
    39961 aagttcctga aggtaagaaa ctttcgttca gggatatgct gagcgctatg actcagatga
    40021 ttgggatgtc ttgcttcttc aaccgagaag gaaagatgga aatccgtgat ttgaccgagt
    40081 ccaacatcac gattaacgct gacagttact tcttgcatgg attgaccaag agtgaaatcg
    40141 agtatcagat agctggtatc acttgtaaga cggataagaa gtctctgacg gtcggtatga
    40201 caacaggtcg gtctttggaa cttgataatg tcttcataac ccagagcgct ttaaatgact
    40261 tgtattacaa actgaaaaac ctaacttact atccgtataa tctcaactac caaggacatt
    40321 tgttacttga ggttgggcag tgggtaacca ttcagaccaa caagaaagag acttttaaag
    40381 ttcctgtctt aagtcagagc tttatcttta aaggtggtct gagaggtcgt atcagtgcag
    40441 atagtaaggc tggaaacgat acccagtatt cttacgaggg tacgattacc aagcagatta
    40501 agcaacaaga tggctttgaa gcgaaaatcc aagcgcagat tgaagcagca gataaagatt
    40561 ttgaccaaaa ggtcgacaaa atcaaaaaag actttaacga tcaagtagaa ctggccaaag
    40621 ccagagctga agaagtcaag agagaactgt ctgacactat caatcagcgc tttaatagct
    40681 ttgacaacgg gccattgaaa gaagctaagc gcaaggctga ggaagctttg cgaaatgctg
    40741 gcgcaagtag ttctcttgct caggaatcca agcggattgg gctggattct gttgctagac
    40801 ttgaagcgtt taagtcgcag actacgagcg cacaaacggc tctgtcgggt gacttggatg
    40861 ttctaaaacg aactatcgca aacgatattc gaccgaagca agcacaggct gaagctgaga
    40921 ttgccaagca agttgaagca cttagccgga ctaaaaatga actggatggc gcaagtaccc
    40981 tgcttgcaca ggaagctaag cggattgagc tggattctgt tgctagactt gaagcgttta
    41041 agtcgcagac tacgagcgca caaacggctc tgtcaggtga cttggatgtt ctaaaacgaa
    41101 ctatcgcaaa cgatattcga ccgaagcaag cacaggctga agctgagatt gccaagcaag
    41161 ttgaagcact tagccggact aaaaatgaac tggctggcgt gaagtcagcg caagcgacgt
    41221 atgaggagac gacgactcgt agactgtcag aactgaccaa cttggccaat ggtaaagcca
    41281 gcaagtcaga actcacgcag acagctgagg agctagctag tcggattgcg agtgtgcagg
    41341 catccggtcg aaatctattc ttgaactcac tattcaagca ggatatttca aaaacaggaa
    41401 tttggacaac gagtacatat acggctacta tcgatagcga aagtaagtat cttggatata
    41461 acgctcttaa aattataggt ctgaatccat ctggccgtga tggagggaat cccaaggtta
    41521 cttatccagt tctgggtcaa ttcgggaaag taattcccgg aagtacgact aatcaagatg
    41581 taaccattag tttttatgct aaggcaaata aaaatggaat aatgctaaga tctcgattag
    41641 ggaatatcgg atataaaact ggaaatgtga cattgtcgac agaaattaaa cgatatgttg
    41701 tccatattcc aaaaggttgg acaaacgaat ccaagcagac cacaaatgaa tggttgttca
    41761 atttcaacca ggaaggaacc gtttggattt ggatgccgaa gtttgaaata agcgatgtag
    41821 atacttctta ttcagaagct cctgaagata tagaaggtca gatttcaaca gttgaatcga
    41881 cctttaaaca acgagtcaac tcgctcgaag ctggtgtgaa tcgtctgact gaaggcctta
    41941 gaaccaaagc cgatatcagc gcactcaacg tgactgctga aaatatccgg caatctgtga
    42001 agagtcttga gacagacacg cagaacaagc taaatcagaa gttgagtcag gctgaatttg
    42061 aggtgcgagc cggctctatc cgtcaggaaa tcctgaacgc aaccaaggat aaggcagata
    42121 agactttagt tgtatctgaa gctgggaaat tgcgtgaaga attttcaaaa atgaaggtgg
    42181 gaggccggaa tctatggata aaatccaaga cggttggagc tgtaattgaa aaattacctg
    42241 aaaaccatgt cacaggtcaa aaagaatgtt ataggttaga gaacaactct actttaacgt
    42301 tcaaccttga accagatttc agctcaaggt tgtatcaaaa agttactttt agcgcttgga
    42361 tcaagtacga aaatgtagtc caaggtcgaa acttttggaa tgtatttaat tgcttcaaac
    42421 attatctttt tagaaaaaat agtgagaccg gagtacagag tggtccagat tatgctacgc
    42481 ttggtatgta taaaggttcg gcagattgga aatatattac attcacttat gactactctg
    42541 aaaaaacaaa ttttgatcaa ttgaagacat cattgcgatt caatcttgaa ggtgctacaa
    42601 gcggtacagc ttgggtaaca ggaatcaagg ttgaaatcgg tagtgtggcg acggactggt
    42661 caccagcacc agaagacgga gaaaatgagc ttttagtcgc taaaaccgag ttcaagagga
    42721 cagctgatgg cctatctact aagatggcag cagttgagag ctatgttggt caagacggtc
    42781 agcgacagga agccctacag cgctatactc gtgaggaaag cacgagacaa gcgacagcag
    42841 tccgtgagct ggtcaatcgt gatttcgttg gtaaggctac ttatcaagaa gatgttaagg
    42901 gtatcaatca gaggattgaa gctgttaaaa ctagtgcgaa taaagacatc gctagtcaaa
    42961 tcgctagcta tcgtcaatct gtagatggta agttcacgga tatttcaagt cagataacta
    43021 cttataagca agatgtgggc ggtcaaatca gtggtctatc aaatagactt acaagcagtg
    43081 agcaaggaac cactactcag atttcaaata tttcaaatcg gataaacagt aataagcaag
    43141 gcacagataa tcagatttca aatttaaaga ctcaggtcgc tacaaacaag gataatgctg
    43201 aacgacaaat gggtagaata tctgatcagg tttctgcaaa caaagcgaat gctgatagtc
    43261 aatttgcgaa tgtgaccaat caactagcgc gaaaagtaga gactactgac ttccagcgtg
    43321 ttaaggaaac cagtaaactt tacgagcgaa ttttgggcaa tactgaaaat ggaattgcgg
    43381 ataaggttgc tcgcatggct ctgactaatc aactgtttca ggttgaggtt gggaaatata
    43441 gtgtaagcgg ccctaacctc attaagaata gtgattttaa aaatgctacg aatgaatggg
    43501 gctcaactca aaatttagga agattggtta agcatagctt ttatcacaac gggcagaaag
    43561 accttatgcg tttaagtaat gcaactaaaa acgaaaactt tttgtatagt caccgtttta
    43621 atcttgaacg aaatactgac tatgtactga attttagagg atttaacaac agtgctctcg
    43681 caagctatga tgtttatatt ttgggacgaa gagcaggcga gagcgatgga ttcacaatcg
    43741 ttaagaaagt tgttagcagc aagaaactat ctacctctag atgcgaagat gtctcagtaa
    43801 cttttaattc cggagaaatg gataatgctt acattcgttt tgataacaat ggctcatcat
    43861 caggaacagc tgatttgtat attacagaag ttgacttgta caaaggttat aaacctagaa
    43921 catggcaacc acatccagaa gatgcagtcg cagatgcgaa taagaagctt gaagcaacgc
    43981 aaacaaaaat gactcaacta gctggctcat gggtagttga aaacatcaac tcggctggag
    44041 atatcatctc tggaatcaat cttggcgcca atggacataa ccgcttcgtt gggaaattga
    44101 cccacatcac tggagagacc ctgattgaca gagcagtcat caagtctgcc atggttgata
    44161 agctgaaaac gggcaatttt gaagctggtt cggtcacgac tacgatatta gacgctgaag
    44221 cggtaactgc tgagaagttg aaagttgacg atgcgcttat tagaaaatta actgcaaatg
    44281 atgcttttat tgaccgactg acatctaaac gtatcttctc tactaaggtt gagtccgtca
    44341 tttctagttc aaccttccta gaagcctatc aaggtcgaat tggtggattc acacttggtc
    44401 aatttgacca gggtggcggt cgctggattt caggtgtcaa tcagttctct gttggtatgg
    44461 ggaatggtgc cgggcatgga gtccggacag ccttctgggc gaactgggga aataattgga
    44521 actatgccgg acctaaagca tggaacgtca atactgatgg gaaaatgtac tgtaggaatg
    44581 aagtcggttt ttatgatcaa gtggattttt cgaattcatc gagagcaaac ttctatggga
    44641 atactacttt ttctcgttct cctgtgtttt caaatggtat cgaacttgga agtaaagatg
    44701 tgcttggtga tggttggaat cccaaaggcg gaaggaatgc ggttgtttgg tggaatcagg
    44761 tcggtagcgg tagcgtgaag tattggatgg aacaaaaatc agacagacgc ttaaaagaga
    44821 acatcacaga tacagctgtg aaagccttgg acaaaatcaa cagattaaga atggttgcat
    44881 ttgatttcat cgaaaataag aaacatgagg agattggtct aatagctcaa gaggctgaaa
    44941 ccatcgttcc aagaattgtc tcacgagatc ctgagaatcc agatggctat ctgcatattg
    45001 actataccgc tttagttcct tacttaatca aggctattca agaattaaat caaaaaatag
    45061 aaaaaatgga gaaaacaata gcatgaataa caacatgttg accaatatcg cactttaaag
    45121 caattcagga gcttgctctt gaaaatagaa aacgaacaca cagattggag aacttagaaa
    45181 atgaacacag aacagcttaa ccaagcctta caaatgacaa ttagtgaaat gtcaacaact
    45241 tcaacaaatt cgatgattac aaataatatc ttgagtattc agttggatga gcaaagggaa
    45301 gggaatcaaa gacttcaagc acgagtggat gagctggaag ctctgcttga tgaacaaact
    45361 aaaccagcag acaaaggaga atagacatgg cagaaacaaa tcaaaacaca gataacttac
    45421 tagaccttac aaaaatcaca gaaccatttg atcttgcgag tgctttgcgc tacatgaaag
    45481 aaaatggaga gttcattcgt tgcaagaatg taagcgatga cttctatatg tatcgtgatg
    45541 ttcaaaaacg tcctgtgatc gtaaatggcc gtcgccaact caaggatgtt gaaaccgttt
    45601 gggcgttcaa ccagtggggt ggtacaatcg caacaatcaa cgtcgccgtt ctgttgaatc
    45661 atgaattcta tatcatgaaa tttgatgcag agggcaatcc tgactggacg gatccaacgg
    45721 taaaacctaa agaataggag gtgtgtatgc caattgaaga agctgaaaaa atcgctcaaa
    45781 gtcaggtagc ttgggcgatt ttgtttatct tgcttttctt tattatcatt cgatatctta
    45841 tcaagacttc ggacaagcga gagaagaaga ttatggattt gcacgagcaa tcaaaggccg
    45901 actctaatag acgagaagag cgtttgatga ctcacctaga aaagaccact acagaattaa
    45961 ccacaatcac tcacgcggtc ggagacattc aaaaagaaat ggttcgcatg aacgaccgca
    46021 tggaagaaat cgaaaaagga gaataacaaa tgcaacaaat tactgaaatc attactaatg
    46081 gagcaatcag catccttgtt attttggctg gtatcgcagt caaggcagtc aaggactacc
    46141 tggttcaaaa aggtggagaa aagaccatca aaatcgttga aatcttggcc aaaaatgcag
    46201 taaatgccgt tgagcaagta gctgctgaaa ctggctacaa gggagatgaa aaactggcac
    46261 aggctcgcgc taaagtccgt gctgagctta caaaatacaa tattagcatg actgacaaag
    46321 acttagacac cttcgtagag tcagcagtga agcagatgaa tgacgcatgg aaaggacgat
    46381 agggaatgga tatcgataga aacagactac gtacaggctt gccacaggtt ggggtgcagc
    46441 cttatcgaca agtacacgcc cattcaacag gcaaccgtaa ctcaaccgta cagaatgaag
    46501 cggattatca ctggcggaaa gacccagaat taggtttttt ctcgcacgtt gttgggaacg
    46561 gtcgcatcat gcaggtagga cctgtgaaca acggaagttg ggatgttggg ggcggttgga
    46621 atgctgagag ttacgcagcg gttgaactga ttgaaagcca ttcaactaaa gaagagttca
    46681 tgacggacta ccgcctctat atcgaattgc tacgcaatct agcggacgaa gcaggcttgc
    46741 cgaagactct tgatacagac gacttggcag gtatcaagac gcatgaatac tgtaccaata
    46801 accaaccaaa caaccactca gaccatgtgg atccatatcc atatcttgct aaatggggca
    46861 ttagccgtga acagtttaag caagacatcg aaaacggctt gagagctgca acaggctggc
    46921 agaaaaatgg cactggctac tggtacgtac actcagacgg ctcttatcca aaagataagt
    46981 ttgagaaaat caacggtacc tggtattatt tcgatggctc aggctatatg ctttcagacc
    47041 gctggaagaa gcacacagac ggtaattggt actactttga ccaatcaggc gaaatggcca
    47101 caggctggaa gaaaatcgct gagaagtggt actattttga tgtagaaggt gccatgaaga
    47161 caggctgggt caagtacaag aacacttggt actacttaga cgctaaagaa ggtgctatgg
    47221 tatcaaacgc cttcgtccag tccgcagacg gaacaggctg gtactacctc aaaccagacg
    47281 gaacactggc agataagcca gacttcacag tagagccaga tggcttgatt acagttaaat
    47341 aaatagaaag gaaactttct aaattgttct ttcaccgcag gctcaggctt gcggtttttt
    47401 tgttttaaaa aaggggcaaa aaaggggcaa aagtgtcgta aatctctgta aaatgatgta
    47461 aaaacattta tttaaaagct caaaatatag ctgttttaaa aggtattgta agatatagta
    47521 aaacgatgta aaggtatttt taaatgcgga tgaattataa aaccacgaat cctatgtgac
    47581 tcgtggttct tttttataaa ctggtagagt gttttggttg tactttttgt tcagggtcaa
    47641 tgtagttgat agcgttgttg acagcagttg gagcttcccc tagacctgtc gcaatcagat
    47701 caatttttcc gtcatagtag cagcagtcac cgatagcata gatacctgct tggctggatt
    47761 cttgtttgct gttgacgata atcttgtgac ggttgaggtc gagcccccag tttttaaggt
    47821 taccgacaga agatttgaaa ccatagttga caaagaggtg gtctaggtca atggtttcag
    47881 tttcatcaga tttgactttt gtgatttcaa gtttatcaag cgtttttcca tctccaagga
    47941 gttggctagg gacgaatggt gtcttgatgg ttacagatga ttcttgcaag gcttgaacac
    48001 tgtgttccaa ggcacggaaa ttatctctgc ggtgaacaag ggtagttggt gcgatttttt
    48061 caaaagccaa agtccaatcc acagccgagt ctcccccgcc aagaatcgtc actttcttac
    48121 cagcgtattg ctgaatgtta gaaacgtggt agtggatatt ttcatagccc tcaacacttt
    48181 caagttccag cggacgtggt ttgaaggcac cgccacccat agcgatgata actgttttag
    48241 tcaggtgact tcctttagaa gttgtgatgg caaattcttc ttcttgtttg tcaatctcaa
    48301 gaaccgtttc attgagatga ataggggtat caaatccatt tagctgttca atcaagcggt
    48361 tagtcaactc ttctccagtc aggtttggga agcctggtac gtctaggatt tccttttcag
    48421 ggtagagaat agcaggttgt ccacctagct ggggaagaga gtcgatgatt tgaaccttgg
    48481 cttggcgtag gtgggcataa aaggctgcaa aaagcccgac aggaccacca cccacaatgg
    48541 taatatcata gagttgagac atagtttctc ctttgttttt tctagtcagt ttattttatc
    48601 atatttttgt tcaatgtaaa atggactagg atagggaaaa aaatgccaga aaatggtata
    48661 ataggacgaa agtgttttga tagggaggat atcagggatg aattttcaac aattatccaa
    48721 tctgcaatat tggaccagtt tgtttgcaag tccatggacg atagctatca atctgattga
    48781 tattttgatt gttgcttata ttttatacca ttttacaaaa gctattgcag gaaccaagat
    48841 tatgattttg gtacgtggag ttttggtgtt tattttagct caaatccttg caaatatgat
    48901 tggtttgact acgatttctt ggttaatcaa tcaaattatt acttatgggg ttattgcggc
    48961 ggttgttatc ttctctccag agattcggac tggtttggaa cgtttgggaa gagcgacaga
    49021 ttttttttcc aatgccccta ttagtgctga ggaacagatg attcgtgcct ttgttaagtc
    49081 tgttgaatac atgagtcctc gtaaaatcgg ggccttggtt gctattcagc gtgtacgtac
    49141 cttgcaggag tatatttcga caggaattcc cttggatgct aagatttctg cagaacttct
    49201 cattaacatt tttattccca acactcccct acatgatggt gcggtgatta tcaaagaaga
    49261 acgtatcgct gtgacgtctg cctatctgcc cttgacaaaa aacacaggga tttccaagga
    49321 atttgggacc agacaccggg cggctatcgg tttatcagaa gtctcagatg ccttgacttt
    49381 tgtcgtatca gaggaaacgg gaggaatttc gataacctat aatggaaggt ttaagcacaa
    49441 cctaacactt gatgaatttg aaacagaatt acgtgaaatc ttacttccaa aagaggaagt
    49501 gggtcttagt tttaaagaac gattgctagg aggatggaaa catgaaaaaa aatagtttat
    49561 atatcatatc ctcactcttt tttgcttgtg tcttatttgt ctatgctacg gcgacgaatt
    49621 ttcaaaacag taccagtgct aggcaggtaa aaacggaaac ctatactaat acagtaacaa
    49681 atgtccctat tgacatacgc tataatagtg ataagtattt tattagcggt tttgcttcag
    49741 aagtatcagt ggtcttgact ggtgcaaatc gcctatcgct agctagtgaa atgcaagaaa
    49801 gtacacgtaa attcaaggtt actgctgacc taacagatgc cggtgttgga acgattgaag
    49861 ttcctttgag cattgaagat ttacccaatg ggctgaccgc tgtggcgact ccgcaaaaaa
    49921 ttacagtcaa gattggtaag aaggctcaga aggataaggt aaagattgta ccagagattg
    49981 accctagtca aattgatagt cgggtacaaa ttgaaaatgt catggtgtca gataaagaag
    50041 tgtctattac gagtgaccaa gagacattgg atagaattga taagattatc gctgtcttgc
    50101 caactagcga acgtataaca ggtaattaca gtggttcagt acctttgcag gcaatcgacc
    50161 gcaatggtgt tgtcttaccg gcagttatca ctccgtttga tacaataatg aaggtgacta
    50221 caaaaccagt agcaccaagt tcaagcacat caaattcaag tacaagcagt tcatcggaga
    50281 catcttcgtc aacgaaagca actagttcaa aaacgaatta aaaatagaaa aggattttat
    50341 aacaatgggt aaatattttg ggactgatgg agtccgtgga gaagctaacc tagaactaac
    50401 accagaatta gcctttaaac taggacgttt tggaggctat gttcttagtc aacatgaaac
    50461 ggaagcgccg aaagtctttg taggacgtga cacacgtatt tcaggggaaa tgctggaatc
    50521 ggccttggtg gcaggtctcc tttcagtagg gattcacgta tacaaacttg gtgtccttgc
    50581 aacaccagca gtagcttact tggttgaaac tgaaggagca agtgccggtg tcatgatttc
    50641 tgctagccac aacccagccc ttgataacgg aatcaagttc tttggcggtg atggcttcaa
    50701 actagatgat gaaaaagaag cagaaattga agccttgcta gatgctgagg aagacactct
    50761 tcctcgtcca agtgcagaag gcttaggaat cttggtagat tatccagaag gcttgcgtaa
    50821 gtatgaagga tatcttgttt caactggaac tcctcttgat ggaatgaagg ttgccttgga
    50881 tacagctaat ggagcagctt ctaccagtgc ccgtcaaatc tttgcagacc ttggtgccca
    50941 attgacggtt atcggggaaa caccagacgg tcttaacatc aaccttaatg ttggttcaac
    51001 acatccagaa gcccttcaag aagtggtcaa agaaagtggg tcagctattg gtttggcctt
    51061 tgatggagac agtgaccgct tgattgctgt tgatgagaat ggtgacatcg tcgatggtga
    51121 caagattatg tacatcatcg gaaaatacct ttctgaaaaa ggacaattgg ctcaaaatac
    51181 aattgtgaca actgttatgt ctaaccttgg tttccacaag gccttgaatc gcgaaggtat
    51241 taacaaggca gttactgcag ttggtgaccg ctacgttgtt gaagaaatga gaaaatcagg
    51301 ctacaacctt ggtggtgaac agtctggtca cgttatcttg atggattaca ataccacagg
    51361 tgatggtcaa ttatcagcag ttcaattgac taaaatcatg aaggaaactg gtaagagctt
    51421 atcagagttg gcggcagaag taacgattta tccacaaaaa ttagttaata tccgagtgga
    51481 aaacgtcatg aaggaaaagg ccatggaagt gccagctatc aaggccatca tcgagaagat
    51541 ggaagaagaa atggcgggga acggccgtat ccttgttcgt ccaagtggaa cagagcccct
    51601 cttgcgtgtt atggcagaag cgcctacaac agaagaagta gactactatg ttgataccat
    51661 cacagatgta gttcgtgctg aaattgggat tgactaaatc ctagaaaact agatgaaaat
    51721 aaaaaattat gatacaatag cttataatat tgtagccgat ggagaaagac atgaatctta
    51781 aacgtgaaca agaatttgtt agtcagtatc attttgatgc gcgtaatttt gaatgggaaa
    51841 atgaaaatgg agctcctgaa accaaggtgg atgtgaattt ccaattgctc caacatgacc
    51901 aagaaaatca agtgacttcc ttgattgtca tcttgagctt tatgattgta tttgataagt
    51961 ttgtcattag tggaacaatc tcacaagtaa accatatcga tggccgtatt gtcaatgaac
    52021 caagtgaatt gaaccaagaa gaggtggaaa ccttggctcg tccatgtttg aacatgctca
    52081 atcgtttgac ttacgaagtg actgaaattg cattagactt accaggaatt aatttggagt
    52141 tctaatatga aattagctgt tttcacagat tcctctgctt atctcagtgc agagaccttg
    52201 caaagagaag acttgtttgt cttggatatt cctgtcaata ttgatggtga ggaatatgtc
    52261 gaaggcatca atctgtctgc tgaggaattt taccaaaaaa tggctcaggc ttctgaattt
    52321 cctaagacca gtcaaccaag tattgccaag ttagatgaaa tcttaacttc gctcaaagaa
    52381 caaggctata cacatgcctt ggggcttttc ctatcttctg gaatttcagg tttttaccaa
    52441 aatatccagt atatggtcga tgactatgag ggcttaacca ttgctttccc agacactttg
    52501 attacaagtg ctcccctagg catcatggtt gaaagcgtct ttaattggcg tgatcagggc
    52561 gatgattttg ccagcattca ggataagcta gccattcaaa tcagccgtac gtcagccttt
    52621 atcatggtag atgacttgga tcatttggta aaaggtggac gcctttcaaa tggggctgcc
    52681 attttgggca atttgcttag cattaagcca atcctttatt ttaacgatca aggtgtgatt
    52741 gaagtttacg aaaaagttcg tactgaaaag aaggccacca agcgcttaat tgaaattatc
    52801 aaggaaacaa cggcttcagg tcaataccgg gtcattgtca ttcatgggaa tgctcctgaa
    52861 aaggctgaag aattgcgtca gcacttgctt gattttggct tgggttcgga tgtttcactt
    52921 gctacatttg gtagtgtcat tggaacgcac ctaggagcag gaagtattgc tctgggttat
    52981 attccagtga tttagttggc gcttctaggc tagttaagat gccttgtatc ttagaaattg
    53041 aacacggact acctgcctct tgaaaaaaga tgcctgtctt gcctttcgtg gaaagtcagt
    53101 gccattccct atttttcaga ggcagctaag gtcctttgta tcttgataat tgaacacgcc
    53161 tggaaccctg tgtgaaaaag atagttcttc caagaagtag acacttcttg attggaattc
    53221 ctattttcac tttgtgttct tacggacttt gtatcttaga caggagtaga gatgagtatt
    53281 cgagtaatta ttgccggttt taagggaaag atgggccagg ctgcttgtca gatggtattg
    53341 actgatccag acttggactt ggtggcagtt ttggatcctt ttgagtctga gtcagaatgg
    53401 cagggtattc ctgttttcaa ggataaggct gatttagctg gttttgaagc ggatgtctgg
    53461 gtagatttta ctactccagc tgttgcctac gaaaatacac gttttgctct tgaaaatggc
    53521 tttgctccag tagttggaac gactggtttc acgagtgaag aaattgcaga gctaaaagaa
    53581 ttttctcgtg cccaagactt gggtggcctg attgccccta actttgcctt gggtgctgtc
    53641 ttactcatgc aatttgcgac gcaggctgcc aaatatttcc caaatgtgga gattattgag
    53701 ctccatcatg acaagaaaaa ggatgctccg agtggaacag ccattaaaac agctgagttg
    53761 atggcagagg ttcgagagtc cattcagcaa ggtgcagcag atgaggaaga gctgattgct
    53821 ggtgctcgtg gtgctgactt tgatggtatg cgcatccact cagttcgttt gccaggcttg
    53881 gtagctcatc aggaagtcat ctttggcaat cagggagaag ggttgaccct ccgtcatgac
    53941 tcctatgatc gcatctcctt catgacagga gtcaatttgg gaattaaaga agttgtcaag
    54001 cgtcatgagc ttgtctatgg attagaacac ttattatgag attaatgcaa atgccttctg
    54061 aatttcagaa ggctttacca gtattagaaa aaattaaaga agcaggcttt gaggcttatt
    54121 ttgttggggg ctctgttcga gatgcccttc tccatagccc tatccacgat gtggatattg
    54181 cgacgtcttc ttatccagaa gagaccaagc agatttttcc gcgaacagcc gatatcggaa
    54241 tcgagcatgg aaccgtcttg gtcttagatg gggatgagga gtatgaggta acaacctttc
    54301 ggacagagga tgtctatgtg gactatcgca gacccagtgc ggtttccttt gtgcgctcgc
    54361 tagaagaaga cctcaaacgc cgtgatttca cagtcaacgc ctttgccttg gatgagacag
    54421 gagaaatcgt tgacttgttc catggtttag aagatttgga aaagcaagtc ttgcgagcag
    54481 ttggagtggc tagtgagcgt ttcaacgaag atgctttacg gattatgcgt ggtttccgtt
    54541 ttcaggctag tcttggtttt gcacttgagc cagaaacatt taaagctatg aagaccttga
    54601 cgccgctttt ggagaaaatt tctgtagagc gtaccttcgt cgagtttgat aaactcttgc
    54661 tggctccatt ttggagaagg ggcttggctt ccatgattga gagtcaagct tatgactatc
    54721 tccctgatat ggcatctagc caggacaagc tcaacagact gtttgatttg gagactgatt
    54781 ttacttttga atcctctgaa caagcctggg cggctttact atgggctttg gagattgaaa
    54841 atgcgcagtc atttttgaaa tcttggaaga cctcacgcca gtttgccaag caagttcagg
    54901 atttgctgat tattttggct ctgcgtgaaa atggagaatt gagtaagcgt gattgttatc
    54961 gctttgactt ggatttactt ttacaggctg aaaatcttcg tcaagctcaa ggaaaagaag
    55021 tcaacccaca agtcattaaa gaaacctacc agagcttgac tattcatgat aagaaagaaa
    55081 tccaaatcaa cggcggtatt ttgatcaagg aatatggcta tcagccaggc ccagacttgg
    55141 gagagatttt aacagagatt gagtttgcca ttgtcgatgg agaattggag aataatcgtg
    55201 aagccatcca tgcttacctg agggagaaaa aatgagtgat tttatcgttg aaaaactaag
    55261 taaatccgtt ggtgacaaga ccgtttttag ggatatttcc tttattatcc atgacttaga
    55321 cagaattggt ttaatcggtg tcaatgggac tggcaagacc acccttttgg acgtcctttc
    55381 tggtgtttct ggatttgatg gggatgtcag tcctttttca gctaaaaatg attaccagat
    55441 tggttacttg actcaggatc ctgattttga tgatagaaag acagttttgg atacggttct
    55501 atctagtgaa ctcaaggaaa tccagctcat tcgtgagtat gaattgatta tgctcgacta
    55561 tagtgaggac aagcaggcgc gtttggaacg tgtcatggca gagatggact ctctccaagc
    55621 ttgggaaatc gagagtcagg tcaagaccgt tcttagcaaa ttgggcattc aagacttatc
    55681 tactcctgtt ggggaattgt caggtggtct gagaagacgg gtacagttgg cacaagtctt
    55741 acttggcaac cacgaccttt tgcttttgga tgagccgacc aaccatctgg atattgcgat
    55801 tattgagtgg ctgaccctct ttttgaaaaa ttctaagaag accgtccttt ttatcactca
    55861 cgatcgttat ttcttagacg ctttgtcaac acggattttc gagttggagc gagcaggctt
    55921 gaccgagtac cagggaaatt accaggacta tgttcgccta aaggcggaac aggatgagcg
    55981 cgacgcggct cttcttcaca aaaaagaaca actctacaaa caagaattgg cctggatgcg
    56041 cagacaaccg caggcgcgtg cgaccaagca gcaggcccgt atcaatcgat tccatgatct
    56101 gaaaaaggaa gtttcaggca gtagtgctga gacagacttg accatgaact ttgaaaccag
    56161 tcggattggg aagaaagtca tcgagtttca ggctgtttcc tttgcctatg aaaacaagcc
    56221 tattttgcaa gattttaatc tcttggtgca ggccaaagac cgtatcggaa tcgttgggga
    56281 caatggtgtt ggaaaatcaa ccctgctcaa cttgattgca ggaagtcttg agccaactaa
    56341 aggacaagtt gtgattgggg aaactgttcg catcgcctat ttctctcagc aaattgaggg
    56401 tttggatgaa agcaagagag ttatcaatta cctgcaggaa gtggcagaag aggtcaagac
    56461 cagtggcggt tctacgactt cgattgcaga gttgctggag cagttccttt tcccacgttc
    56521 gacgcatgga accttgattg agaaattgtc agggggtgag aaaaaacgtc tttatctcct
    56581 caaactgctt ttggaaaaac caaatgttct tcttttagac gagccaacca atgacctaga
    56641 tattgcaact ttgacagtct tagagaattt cttgcaaggt tttgcaggtc ccgttttaac
    56701 agtcagtcac gaccgctatt tcttggataa ggtagcgacc aagattctcg cttttgagga
    56761 tggcaagatt cgtcctttct ttggtcatta caccgactat cttgatgaaa aagcttttga
    56821 aacagatatg gccaatcaag tgcaaaaggt cgaaaaggaa aaagtggtca aggttcgaga
    56881 agacaagaaa cgcatgacct accaagaaaa gcaggagtgg gcaagtattg aaggtgatat
    56941 tgaaaccttg gaaaaacgta tcgctgctat tgaagaggaa atgcaggcta acggctctga
    57001 ctttggtaag ctgactactc tccaaaaaga attggatgag aaaaatgaag cactccttga
    57061 aaaatacgaa cgctatgagt atctcagtga atttgatagt taagactatt cgaaaatcaa
    57121 agtgcaaact atgtcggctt cacattgccg actcaagtac agcctgtgac cggcttccta
    57181 atttgcactt tgatttttat tgagtataaa agaatagaaa aatcccgtct catgaaagga
    57241 tttctctatt cttttttggt ttcttgatga aagatatttt gccaggtttc gtggcgacgc
    57301 cagatactgg tatggatgat accatctaac tcatagcaga tgagtttggt tttttgactg
    57361 atggaagtaa tttgaatgtc ctttatagca gaatttaact cttttttggt cttataagcc
    57421 tctttatcca tctgctctcc atcttggcga atatagacaa aatcatcagt caagagttct
    57481 tctagttggt ttccttggtc aatcaattgt tcacgcataa gcaatttttt atagacattg
    57541 tctaaaattt catcctcagg gataggtgct ggttcgatat ggacatcggt atcaaagacg
    57601 ccaaaacgct cctccagcat agactcgacc tgatccgcga tttcatggct ttcaaaaaca
    57661 gacaagtcag gattcatctc tagtgtaata tccaggtaga tgttgctacc gtaggtgcga
    57721 cctctttgcg atttgacctt gctgattttg ggaatttcca tgatagcctt ttggtagtcc
    57781 tcgagcaggc ggtcgtcaaa gccatctgaa agactaaagg aagactcgat gaagatatca
    57841 taggcagtct tcaagataaa gaaagtgatg atgatagcaa ccagtttatc cacaatcgga
    57901 taattgaaac tactagctag gatggcaatg gcggttccaa gtgaggtaac agcgtcagaa
    57961 agattgtcct tagcagctgc cttcagcgcc ttggagttgg atttcttact gaggcgagta
    58021 ttgtagagat agaccacaaa cataatcgct gcagaaatga ttcctagagt tgcaccaaga
    58081 ggatcaatga ccgtttcttc ccgactgaga atcttttgaa tggtatctct tagaacatcg
    58141 aaaccgacat agaacatgat gatagaagtg atcaagcttg ccaaatcttc aatcttccaa
    58201 tgaccaaaac ggtggtcacg gtctgcaggc tggcgcgcca tccgaatccc gattaagagg
    58261 gccacatttc caatgatgtc cgatacgtta ttaaaaccat cggccaccaa actggatgaa
    58321 tgaaggagat gaccagctgc taatttagct gcagacaaga tcaaataggt cgaaatactg
    58381 ataatggctc cacgctcagc taacttgaga tttgagatag attgcttcat tcaacttcct
    58441 ttctagtcac atagcattct accattatac tggttttcaa ggaaaaattc aaataaggca
    58501 gtttttactc tttgaaaact ccccaaaatt tggtataatg gtactactca agggagtagc
    58561 tggcagaaac ctgtgatagt gtcgtcattc cgaattttat gctgaaaagt atgctttccg
    58621 gccctatctt aaacagcgag acttgttatg attaacaggt ctctttttgt ttgtcgtgaa
    58681 aagatatgac agaaagagag tctgttttgc acacaatgtc aaggaggaga cacatgtcaa
    58741 aagaacaaaa acgccaagcg ttttatactc agagccctga agaggtcttg caggctgtgg
    58801 atgcgaccga gcaaggtttg tcatcaagtg aggcggaaaa gcgccttgcc gaatttgggc
    58861 acaatgaact cgaagaaggc gagaaacgat caatcttggt caaattcatc gagcaattta
    58921 aggatttgat gattatcatc ctagttgcgg cagcaatctt gtcagtcgtg acttctggtg
    58981 gggaagatat cgcagatgcc attatcatcc tagctgtggt tatcatcaac gctgcctttg
    59041 gtgtttacca agaaggaaaa gctgaagaag ctattgaagc cctcaaatcc atgtctagtc
    59101 cagttgcccg cgttcttcgt gatggacaca tggcagagat tgactctaaa gaattggtac
    59161 caggcgatat cgttgccctt gaagcaggtg acgtggtacc agcggaccta cgtttgatag
    59221 aagccaactc tcttaaaatt gaagaagcag ccttgacagg tgaatctgta ccagtcgaaa
    59281 aagacttgtc agtcgagctt gcgacagatg ctggtattgg tgaccgtgtc aacatggcct
    59341 tccaaaactc aaacgtaacc tatggtcgtg ggatgggtgt tgttgtcaat acaggtatgt
    59401 acactgaagt tggtcatatt gctggtatgc ttcaagatgc ggatgagact gatacaccac
    59461 tcaaacaaaa cttgaacaac ctttctaagg tcttgaccta tgctatcttg gtcattgccc
    59521 ttgttacttt tgtagtgggt gtcttcattc aagggaaaaa tccacttggt gagttgttga
    59581 cttctgttgc ccttgccgtt gcagccattc cagaaggact tcctgctatc gttaccatcg
    59641 ttctttctct tggtactcaa gttttggcca aacgacattc catcgttcgt aagttgccag
    59701 cagttgaaac acttggttca actgaaatca tcgcttctga taagactggt acgctgacta
    59761 tgaacaagat gacagtcgaa aaagtcttct acgatgcggt tctacatgac tcagctgatg
    59821 atattgaact aggtcttgaa atgccactac ttcgttcagt tgtcttggcc aatgatacga
    59881 aaatcgatgt ggaaggtaac ttgattggtg acccaaccga aacagccttt atccaatatg
    59941 ccttggacaa gggctatgat gtcaaaggtt tcttagagaa atatcctcgt gtagctgaat
    60001 tgccatttga ctctgaccgt aagctcatgt caacagttca cccattgcca gatggtcgtt
    60061 tccttgtagc agtcaagggt gcgccagacc aactcttaaa acgttgtctt cttcgtgata
    60121 aggctgggga tattgctccg attgatgaga aggttacaaa tctcattcat acaaacaatt
    60181 ctgaaatggc tcatcaagcc ttgcgtgtcc ttgcaggtgc ttataagatt atcgatagta
    60241 ttccagaaaa tctcacttct gaagagcttg aaaatgattt aatttttact ggtttgattg
    60301 ggatgattga ccctgaacgt cctgaagccg ctgaggctgt tcgtgtggct aaggaagcgg
    60361 gaatccgtcc aattatgatt acaggtgacc accaagacac agcggaagcc attgccaaac
    60421 gtttgggaat cattgacgca aacgatacag aaggtcacgt tttaactggt gctgaactca
    60481 atgaactgtc agatgaagaa tttgaaaaag tcgttggtca atactctgtt tatgcccgtg
    60541 tgtctccaga acacaaggtt cgtatcgtca aggcttggca aaaacaaggt aaagtcgttg
    60601 ccatgacagg tgacggtgtc aatgacgcgc cagctctgaa aacagccgat atcggtatcg
    60661 gtatgggaat cactggtaca gaggtttcta agggggcttc tgatatgatt cttgcagatg
    60721 ataactttgc gactattatc gtcgcagtgg aagaaggacg taaggtcttc tcaaacattc
    60781 aaaagactat tcagtaccta ctttctgcta atactgctga agtattaacc atcttcctat
    60841 caaccttgtt tggttgggat gtcttacagc cggttcatct tttgtggatc aacttggtaa
    60901 cggatacctt cccagctatc gctcttggtg ttgaacctgc agaacctggc gtcatgaatc
    60961 ataaaccacg tggacgcaag gcaagcttct tctcaggtgg tgttttgagt tctatcattt
    61021 atcaaggtgt actccaagca gctcttgtta tgagtgttta tggccttgcg attgcttacc
    61081 cagttcatgt gggtgacaat catgctattc atgcagatgc cctaacgatg gcctttgcaa
    61141 cccttggttt gattcagctc ttccatgcct acaatgtcaa gtctgtctac caatccatct
    61201 tgacagttgg cccattcaag tctaagacct ttaactggtc catcttggta tcctttatcc
    61261 ttctcatggc aacaatcgtt gtagaaccgc ttgaaggaat cttccacgta accaaactag
    61321 acttgtcaca atggggaatt gttatggctg gaagcttctc aatgattatc atcgtcgaaa
    61381 tcgttaagtt tattcaacgc aaactcggtt ttgacaagaa tgcgatttaa gggaggtttc
    61441 ggatgtcagc atatcaatta ccgaccgtat ggcaggatga agctagtaat caaggagctt
    61501 ttacggggct aaacagacca acagcaggtg cccgtttcga acaaaacttg ccaaaaggag
    61561 aacaagcttt tcagctttat tcactgggaa caccaaatgg tgtgaaggtt actatcttat
    61621 tggaagaatt actagaagct ggttttaagg aagcggctta cgacttgtat aagattgcta
    61681 tcatggatgg ggatcaattc ggctcagact ttgtgaagct caatccaaat tccaagattc
    61741 cagccttatt ggaccagtca ggtactgaaa gcgtaagagt ctttgagtct gctcatattc
    61801 ttctttacct tgctgagaaa tttggagcct ttttaccaag taatcctgtg gaaaaggtag
    61861 aagttttgaa ttggctattc tggcaagcag gtgcagcacc ttttctaggt gggggatttg
    61921 gacatttctt caattatgct cctgaaaaat tggaatatcc tattaaccgt tttacgatgg
    61981 aagtgaaacg ccagttggat ttattggata aggaattggc tcagaaacct tatattgcag
    62041 gcaatgacta tacgattgca gatattgcta tctggtcttg gtatggacag ttagttcaag
    62101 gaaatcttta ccaaggttct gcaaaattct tggatgcctc aagttatcaa aatctagtaa
    62161 aatgggcaga aaaaattgcc aatcgtccag ctgttaagcg tggattggaa gtaacttata
    62221 cagaaattaa atagcaaaaa agtcatcttc ggatggcttt ttgctacagt tgagggaaag
    62281 ggtttgcagt tatcggcttt tgtgctataa ttgctataat atagtaaaaa gcaagaggag
    62341 tgtgaggaag tggaaaagaa aattgtgaag gatatcttat ttttatctca agtgtctcag
    62401 ccggcaagtc aggaggacct ttatcttgcc agagatttgc aggatacact cttagcaaat
    62461 cgtgatacct gtgttggtct agctgccaat atgattgggg tgcagaagcg cgtgattatc
    62521 tttaatcttg gcttggttcc cgtggtcatg tttaacccag tgcttctgtc ctttgaagga
    62581 tcttatgagg cagaagaagg ctgtttgtcc ttggtaggtg tgagatcaac taagcgttat
    62641 gaaaccataa ggcttgccta tcgtgacagc aagtggcagg aacagaccat taccttgaca
    62701 ggcttcccag ctcagatttg ccagcatgag ctggatcact tggaaggacg aatcatttag
    62761 gaggaaagca aatgaaacga atagtctttg aacttatttt tatcgcaacg acctggtata
    62821 tctttttacc gccccttaac ctgaccagct gggaatttct cttcttcctc tgtgggcatt
    62881 tgttagttgt ggcaatctta tttggctttg gcaaggggat aaaccttgtc aaaacggttc
    62941 atgtgcgcca cggtaaggcg gaagctgcct taaatcttga gggtttcaaa atcaatcggt
    63001 tagggaaaat tctgttagct tcgattggag gaattcttct cttggcagct ttggtttcct
    63061 tggtaacttc cagcatgttt caggctaaaa attatgccaa tgtagtcacg gttacggaaa
    63121 aagactttac tgaatttcct aggagtgaca ccagtaaggt tcctatccta gatagaagta
    63181 ctgctgaaaa aattggagac cgctacttgg gttccctaac cgataaggtg tcgcaatacg
    63241 tagcggcaga tacctatacc caattgacaa ttgatgggaa accttatcgg gtcacaccac
    63301 tagaatatgc agaccctatc aaatggttta acaatcaagc caagggaatc ggtgagtata
    63361 tcaaggtgga catggtaact ggaaatgcgg atttggtgga cttgaagaca ccaatcaagt
    63421 attcagactc ggagtatttt aaccgtgatg tcaaacgtca cctgcgcttg aagtacccga
    63481 ccaaaatctt taaaactcca tcttttgagg tggacgatga gggcaatcct ttctatgtag
    63541 caacggttta ccaaaagcaa tttggacttg ctgttcctcg tcctgcttca gtcattatct
    63601 tggatgctac aaatggagaa accaaggaat acagcttatc agatgttcca gaatgggtgg
    63661 acaggatcta tccagcagag gaaaccattg agcaaatcaa ctacaacggc aagtacaagg
    63721 acggtttctt gaatgccatg atttccaaga aaaacgtgac ccagactacc aagggctata
    63781 attacttgtc tatcggtaat gacatctatc tctacacagg tgtgacgtcg gctaatgcgg
    63841 atgagagtaa tcttggtttc atccttgaaa atatgcgaac aggagaaatc actaagtata
    63901 gcttggcttc tgcgacagaa gaatcagccc gtgaatcagc agaaggtgct gttcaggaga
    63961 aatcctacaa agcaaccttc ccaatcctca tcaacctcaa tgacaagcct ctctacatca
    64021 tgggcttgaa ggacaatgct ggcttggtca aagagtacgc cctggtagac gcagtcgagt
    64081 accaaaatgt tatcgttgct actacagtgg aagagatgct cagcaagtat gccaataaaa
    64141 acgaccttga aattgacaat gcaacgacag aaagcatcaa tggagtagta gcagacctca
    64201 aatcagctgt tatcaaggga gacactgtct acttctttaa agttgatggc aagatataca
    64261 aggtcaaggc ttcagtatcc gatgaccttc cttaccttga aaatggtaaa accttcgaag
    64321 gtcaagtagg aaaagacaat tatctcaaga cctttaagct acggtaaaaa taggtttttt
    64381 tcagaaagta tatgttataa taaggtaaat taagcctaat ggaggacaaa gaaatgggtt
    64441 tttatttgat ggttgcttca atgctactcg gactgctcgc tttgaagata ggtttttctc
    64501 agttcaagga gaaaaaagat aaattcttat ctatcttaac aagtttagct ggcacagccc
    64561 ttgttctcgt ggctgtctgg ttaggatggc ccaaataaat agaaaaacct cggtcaaacc
    64621 gaggtttttc aaatgaattt cttggttgtg gctaaaaaat atgctacact atcaatatga
    64681 aaattttaat cccaacagca aaagaaatga acacagactt gccaagtatc gaggcaattt
    64741 ctttaaaacc agaaagtcag gccgtgcttg atgccttggc tctctattct gccagtcaat
    64801 tggagagttt ctacaaggta tcagctgaga aagcggcgga agaatttcaa aatatccaag
    64861 ctttgaaaag gcaaactact caacactatc cagccttgaa actttttgat gggcttatgt
    64921 accgcaacat taagagagat aagctgaccg aggcggaaca agattatctt gaaaatcatg
    64981 tcttcattac ctcggctttg tacggtgttg ttccagcctt gtcacccatg gctcctcacc
    65041 gtttggattt tttgatgaaa ttaaaagttg ctggtaagac tttgaagagc cattggaagg
    65101 cagtctatga tgaagctctg aagaaggaag aagtgatttt ctctctcttg tcatcagagt
    65161 ttgagactgt attttctaag gaaatcagag caaagatggt gaccttcaaa ttcatggagg
    65221 atagaggcgg tcagctgaag attcactcaa ctatctccaa gaaagcgcgc ggggcctttc
    65281 taacagcttt aatagaaaat caagtacaaa ctgtggggga agcacgtcgc ttgaactttg
    65341 ctggatttgt ttaccgagaa gatttgtcac aaccacaggg attggttttt gtaaaggaag
    65401 tatagaaatc agatattttg tatttggcaa tatgtatcat cagctatttt agtttataaa
    65461 tgagaaaaaa gcgaacaagc tagtcttctg agcgtcagaa aagtagtctt gttcgctttt
    65521 attatgttat actcttcgaa aatctcttca agccatgtca gtgtcgcctt accgtatata
    65581 tgttactgac ttcgtcagtt ctatctacaa cctcaaagca gtgctttgag tagcttgcgg
    65641 ctagcttcct agtttgcttt ttgattttta ttgagtgtta gttacttgat agcttcgacc
    65701 aagtcttgag cttgtttttc aagtgagttt aggactgttt cttcaagaac caattttccg
    65761 tctgcccagg cagagtcatt aacacgtgca gcagtgaaat caccaacgcc ttgtgtacgg
    65821 ataaatggca agaggtcttt gtagatagcg aaaagttgat cgtgccctgc attggctaca
    65881 gatgatactg tgacaaactt gtcttgaagg gcagaaacgc cacgtgtatc agacaagtca
    65941 agggcacgag atagccagtc aagcaagttt ttcactgtac cagggataga gaagttgtag
    66001 actggagaga aaatccagat agcatccgca acgagaactg cttcacgagc agcagctaca
    66061 gctggatgtg ttggaacttc caaatcttgg ctgaagagag gaagggctga ataatcaagg
    66121 tagctaactt ccgctttacc agcaagtgct ttctcagctt cgagcgccat ttggtggttg
    66181 aaagaacctt gacgtagtga tccgacgata aataatactt ttttagacat gatgtgtctc
    66241 ctttttctta aaatttcgag aactctctcg ttgttataaa atatattaca acaaaacaaa
    66301 agaacttgca agaaatttgc tcagagttta tttgaaaatt gtaaaatttc ggaaactcta
    66361 gtcatttgtg actgaaaaag taagaactgt ttcactttca aaatcggttt tgtttgaagg
    66421 gagaaataga attggttgct caaagcacag ctttgaggtt gcagataaag ctgacatggt
    66481 ttgaagagat tttcgaagag tattcttata ttcacttgta ataaagctta atagatggta
    66541 aaatagacga gtgaaaaaaa gacaaaacaa agcaaaaaat aatctactgt ggcagtatgg
    66601 tctagggatg acgattttgt ttgtggttat cagtgcttcc tttctgtata tggtttctct
    66661 tagcatgaaa ccctatcaaa cagctaaaag tgaaggagaa aaattagctc agcagtatgc
    66721 aggattagag caggctgatc aggttgattt atacaatggc ttggaatctt attacagcgt
    66781 tcttggtcgt aataaacagc aagaagcgct tgctgtcctg attggtaaag atgaccataa
    66841 gatttacgtt tatcagctaa atcagggtat ttcacaagaa aaagcagaaa cggtttctaa
    66901 ggaaaaggga gctggcgaga ttgacaagat aacctttggt cgttatcaag acaagccaat
    66961 ctgggaagtt aagtcaggat ctgattttta tctagtagat tttgaaacag gagcattggt
    67021 caataaggag ggcctatgaa actatccaac cgtgttttag aaatggaaga aagtgtgact
    67081 ttggctgctg gagcccgtgc caaagcactg aaggctgagg gtagagatat tttgtctcta
    67141 accttgggtg agccagattt cactactccc aaaaatatcc aagatgccgc cattgcatcg
    67201 attcgagatg ggcgtgcttc tttttataca gtaacctcag gtctgccaga gcttaaggcg
    67261 gtggtcaata gctactttga gcgcttttac ggctattctg tagcgtcaaa tcaagtgaca
    67321 gtcgctgcgg gagccaaata ttctctctat accttcttta tggctgtggt caatccaggt
    67381 gatgaagtca tcatcccaac cccatactgg gttagctatg gagatcaggt caagatggca
    67441 gaaggcgttc ccgtctttgt ttctgctaag gaagacaatc actttaaggt gaccgtagag
    67501 cagttagaag cagctcgcac tgacaagacc aaggttttgg tgctgaattc gccatctaat
    67561 cccacaggta tgatttacac ccgtgaggaa ctcttggcaa ttggaaactg ggctgtagaa
    67621 aatgatattc tcatcctagc agatgatatc tatggccgct tggtttataa tggtcatgag
    67681 ttcacaccga tttctagcct atcggaagcg attcgcaagc aaacagtggt catcaatggt
    67741 gtgtctaaaa cctatgccat gactggttgg cggattggtt atgccgttgg agaagcagac
    67801 attatcgctg ccatgtccaa gattgcaggt caaacaactt cgaatccgtc agcagtagcc
    67861 caatatgcag cagttgaggc tctatcaggt gagcaagata ctgtagaaag catgcgtcag
    67921 gcctttgagg aacgtcttaa taccatctat cccctccttg cagaggtgcc aggatttgaa
    67981 gtggtcaaac cacaaggggc cttctatctc ttcccaaatg tcaaaaaggc catggagatg
    68041 aaaggctaca cggatgtgac agactttaca actgctatct tagaagaagc cgaagtggcc
    68101 ttggtaacag gagctggatt tggagcacca gaaaatgtcc gcctcagcta tgcgacagac
    68161 ctagacacgc ttaaagaagc agtcgaacgc ttgaaagcat ttatgggtag tgagaatgat
    68221 tgatcatttt gagattaagg taaaggattt acaaatttca gaaggatttt ataggagttt
    68281 tctcgctcct ttggactata aattggcttt taagactagt tctctaatta gttttctatc
    68341 cccgaacagc cctcatcctg gtggtgattt ttggctgact cagggaacac aagatcctgt
    68401 tcattttgct tttttagcag aaaataagga agaagttcag gcttgttatg aggctggctt
    68461 agaggcaggt gggcgagaca atggggttcc tggttatcga agtgagcatc cgatttatta
    68521 tgctgctttt atgattgacc tggatgggaa caatatagag gtggtttgcc ataaagaata
    68581 aaaaagaaaa ggaaaaataa tgacaaaacg tgtaacgatt attgatgtaa aagactatgt
    68641 tggtcaggaa gtgacgattg gcgcttgggt tgccaacaaa tcaggaaaag ggaaaatcgc
    68701 tttcttacaa ttgcgtgatg gaacagcctt ctttcaaggt gtggctttta aaccaaactt
    68761 tgtcgaaaaa tttggtgaag aagtgggact tgagaagttt gatgttatca aacgcttgag
    68821 ccaagaaacg tctgtttatg tgacaggtat tgtcaaagag gacgaacgtt ctaaatttgg
    68881 ctatgagttg gacatcacag acatcgaagt gatcggtgaa tctcaagact acccaatcac
    68941 accaaaagaa cacggaacag actttttgat ggataaccgt cacttgtggc tacgctctcg
    69001 taagcaagta gctgtgttgc aaatccgtaa cgctattatc tatgcaactt atgagttctt
    69061 tgacaagaac ggttttatga agtttgacag cccaattctt tcaggaaatg cggcagaaga
    69121 ttctacagaa ctctttgaaa ctgactactt cggaacgcca gcctacttga gccaatcagg
    69181 tcagctttac ctagaagcag gggctatggc tcttggtcgt gtctttgact ttggtccagt
    69241 tttccgtgct gaaaaatcaa aaacacgccg tcacttgact gagttctgga tgatggatgc
    69301 tgagtactca tacttgacac atgatgagtc gcttgacttg caagaagctt atgtgaaagc
    69361 tcttctacaa ggtgttcttg accgcgcgcc tcaagccttg gaaaccttgg aacgtgatac
    69421 agaactcttg aaacgctaca ttgcagagcc attcaaacgt atcacttacg atcaagccat
    69481 tgacctcttg caagagcatg aaaatgatga agatgctgac tacgagcatc ttgagcatgg
    69541 tgatgacttt gggtcaccac acgaaacttg gatttcaaac cactttggtg tgccaacatt
    69601 tgtcaagaac tatccagcag ccatcaaggc cttctacatg aaaccagttc ctggaaatcc
    69661 agagcgcgtg ctttgtgcag acttgcttgc tccagaaggc tatggagaaa ttatcggtgg
    69721 gtctatgcgt gaggaagatt acgatgccct tgtcgctaag atggatgaac ttggcatgga
    69781 tcgtacagaa tatgaattct accttgacct tcgtaaatac ggtacagttc cacacggagg
    69841 atttggtatc ggtatcgaac gtatggtaac cttcgcagca ggaacaaaac atatccgtga
    69901 agctattcca ttcccacgta tgttgcaccg tatcaaacca taagaacagg aaaacgccca
    69961 tgtggcgttt tttcgaattc tagtgttttt acttgcgtaa aaaaattttt tctagtataa
    70021 tagtttattg tgagcgaacc tcacttaccc cttgcaaagt cttggggtca ttagaccaaa
    70081 aggaggaaca tatcaatggc taaatacgaa attctttata tcattcgtcc aaacattgaa
    70141 gaagaagcta aaaacgcttt ggtagcacgt tttgactcta ttttgactga caacggtgca
    70201 actgttgttg aatcaaaaac ttgggaaaaa cgtcgtcttg catacgaaat ccaagatttc
    70261 cgtgaaggac tttaccacat cgttaacgtt gaagcaaatg acgatgcagc tcttaaagag
    70321 tttgaccgtc tttcaaaaat caacgctgac attcttcgtc acatgatcgt caaaattgac
    70381 gcgtaagaag gtaaactatg attaacaatg ttgtacttgt agggcgtatg acacgtgacg
    70441 ctgagttgcg ttatacccca tcaaatgtag cagttgcgac ttttactctt gcagtaaacc
    70501 gtacatttaa gagtcaaaat ggtgaacgtg aggctgattt tataaatgtc gttatgtggc
    70561 gccaacaggc tgaaaatctt gctaactggg ctaaaaaagg ctcacttatc ggggtgacag
    70621 gtcgtatcca gactcgtagt tacgataacc agcaaggaca acgtgtctac gtgacagagg
    70681 tcgtggctga gaatttccaa atgttggaaa gccgtagtgt gcgtgagggt cacacaggtg
    70741 gagcttactc tgcaccaact gcaaactatt cagcacctac aaattcagta ccagactttt
    70801 cacgtaatga aaatccattt ggagcaacaa acccattgga tatttcagat gatgatttac
    70861 cattctaatg gacaactaaa cttataaagg agaaaaaaca tggctcaaca acgtcgtggc
    70921 ggattcaaac gccgtaaaaa agttgattac atcgcagcaa acaaaattga atatgttgat
    70981 tacaaagata ctgagcttct tagccgtttc gtttcagaac gtgggaaaat ccttcctcgt
    71041 cgtgtaacag gaacttcagc taaaaaccaa cgtaaagtaa caacagctat caaacgcgct
    71101 cgcgtaatgg ctttgatgcc tttcgtaaac gaagattaaa gaaaagaccc ggttgggtct
    71161 ctttttcatg agcttgaaaa caagaaagcg aattaagacc ccaatgggtc ttttttcaca
    71221 aactttgaaa tgagagagcg agttaggctc agtggctccc tttttacgag tttgaaaaca
    71281 agagaacaca ttaaaagcac gatggagctt tttcataaat atgtcttttt tctcatccaa
    71341 gaaattaaaa taaaaatgat aaactttttg aaaatagggg atttgcctac tttatgatat
    71401 aatggaaaga gttagattga aagaggtagt gagatggacg cgaaattaag atacaaggca
    71461 aagaagatca agattgtctt ttttgatatt gatgatacat tgcggaattc aaagacaggg
    71521 tttattccaa ctacaattcc cactgttttt aaacagttgc gtgaaaaagg aattttaaca
    71581 ggaatcgcct ctggacgtgg catttttggt gttgttccag agattcgtga tctcaagcct
    71641 gacttttttg taactttgaa tggggcttat atcgaagata aaaaaggtca ggtcatttat
    71701 cagcatcaga ttgaaaagtc agatgttgag gagtatatct cttgggctaa gcaagaagga
    71761 attgagtatg gcttggttgg gagtcatgat gccaagttgt cgattcgcac cgatatgatg
    71821 agtgaagcta tcaatccaat ttatcccgac ttagatgtag atcccgattt ccatgaaaaa
    71881 gaagatatct atcagatgtg gacttttgaa gataagggag atgacttgca cttgcctgac
    71941 agtctctcag acaaacttcg catggttcgt tggcatcaac attcgtctga tattgtgccg
    72001 atttcaggct ccaaagcgac gggggtggaa aaggttgtgg aacaccttgg cttgaaacca
    72061 gagaaggtca tggtttttgg agatggtctc aacgacttgg aactctttga ttatgctgga
    72121 atcagcgttg ccatgggaat ttctcatgat aaaatcaaag aaaaagcaga ttatattaca
    72181 aaaacattag aagaagatgg catttttgat gccttagaag tatttggtat ggtagaaaaa
    72241 gaattgcatt ttccacaagt agacattgaa acagtagaag gtcctcttgc gactattaag
    72301 accaatcacg gagacttacg tatcaagctt ttccctgaac atgctcctaa aacagtggct
    72361 aactttgtat ctctttcaaa agatggctac tatgatggtg tcattttcca ccgtattatc
    72421 aaggacttta tgatccaagg tggagaccca actggaactg gtatgggtgg cgagtcaatc
    72481 tacggcgaat cttttgagga tgaattctca gaagagcttt acaatatccg tggtgctctt
    72541 tccatggcaa atgctggtcc aaataccaac ggcagccagt tctttatcgt gcaaaaccaa
    72601 cacctacctt attctaagaa agaaattact cgtggtggtt ggccagaacc gattgcagaa
    72661 atctatgcca atcaaggtgg gacacctcac ctagaccgcc gtcacacggt ttttggtcag
    72721 ttagctgatg aagcatctta cgctgtcttg gatgccattg ctgctgttga gacaggagct
    72781 atggacaagc cagttgaaga tgttgtaatt gaaactattg aaatcgagga ctaagatgaa
    72841 aatcggtgat aagctaaagg ggcgtattac agggattcag ccctacggtg cctttgttga
    72901 gttagagacg ggtgatacgg ggctgattca catttcagag attcggacag gatttattga
    72961 aaatattcat gaaaccttga aagtcgatga agaggttcaa gttcaggtag tggatttaga
    73021 tgaatttacg gggaaagcca gtctttctat ccgcactttg gaggaaaaaa agtaccagtt
    73081 tcctagacgg agacgctttt caagcgaccg ctttaactac ggttttgctc cctttcgacg
    73141 aatgctacca atctggactg gagaagccct gcaccattta aaaaagaaga agtagtttag
    73201 agttttatat tttttgtaat gttaatatga atttgctata ataggaccat ggaagaagaa
    73261 caattattaa aatcaggaga gcgcattaac cagctctttt cgacagatat caaaatcatt
    73321 caaaatagag aggtttttag ctattcggtg gatagtgttc tcttatcacg atttccacgt
    73381 tttcctaaga aggggttgat tgtggatttc tgtgctggga atggagcagt ggggcttttt
    73441 gctagcactc gtactcaagc acagatattg tctgttgaga ttcaggagcg tttggcggat
    73501 atggctgaac gctctgtccg tttgaatgga ttagaggagc aaatgcaggt catctgcgat
    73561 gatttgaaaa atatgcctgc tcacattcag gggagtaagg tggatatgat tttgtgtaat
    73621 ccaccctatt tcaaggtgga tccttattct aatctgaacg agagtgaaca ttatctcttg
    73681 gctcgacacg aaatcacgac taatttggaa gaaatctgtc gtagtgccca gagtattctc
    73741 aagtctaatg ggcgtttggc catggttcat cgtcctgatc gacttctgga tattttggat
    73801 acgttaaaac gacataatct agcccctaag cgcctgcagt ttgtttatcc aaaaagagaa
    73861 aaggaagcca atatgctttt gattgaggcg atcaaggatg gctcaacaag tggctttaag
    73921 gtcttacctc ctttgattgt ccacaatgat gatggctctt atacgcccga aatcgaagag
    73981 atttattatg gatcataagg cttatatgta cgttctggag tgtcgtgatg gatcctacta
    74041 tataggctat acgactgata tgagaagacg cctcgctatc cacaatagtg ggaagggagc
    74101 caaatataca cgagcacgct tgccagtcaa acttatctat gctcaaggtt ttgccagtaa
    74161 ggaagaagcc atgtcggctg aagctcttct caagcgtaag aagaggccac agaaggaaga
    74221 atttttatct gaaaatcaag atagaaattt actcagttat tttgaagaat cgtggggagt
    74281 cctttgactc ctcttacctt tgtcaaaaag cgaaaaaaat gctacaataa agaaaataaa
    74341 caaaatgagg tattatcatg tccaagattc tagtatttgg tcaccaaaat ccagactcag
    74401 atgccatcgg gtcatctgta gcttttgcct accttgcaaa agaagcttac ggtttggata
    74461 cggaagctgt tgcccttgga actccaaatg aagaaacagc ctttgtcttg aactattttg
    74521 gtgtggaagc accacgtatt atcacttctg ccaaagcaga gggggcagag caagttatct
    74581 tgactgacca caatgaattc caacaatctg tatcagatat cgctgaagta gaagtttatg
    74641 gtgttgtaga ccaccaccgt gtggctaact ttgaaactgc aagcccactt tacatgcgtt
    74701 tggagccagt tggatcagcg tcttcaatcg tttaccgtat gttcaaagaa catggtgtag
    74761 ctgtgcctaa agagattgca ggtttgatgc tttcaggttt gatttcagat acccttcttt
    74821 tgaaatcacc aacaacacac ccaacagata aaatcattgc tcctgaattg gctgaattgg
    74881 ctggtgtgaa cttggaagaa tatggtttag caatgttgaa agctggtacc aacttggcta
    74941 gcaaatctgc tgaagaattg attgacatcg atgctaagac ttttgaactc aacggaaata
    75001 atgtccgtgt tgcccaagtg aacacagttg acatcgctga agttttggaa cgccaagcag
    75061 aaattgaagc tgcaatgcaa gctgccaacg aatcaaacgg ctactctgac tttgtcttga
    75121 tgattacaga tatcgtcaac tcaaactcag aaatcttggc tcttggtgcc aatatggaca
    75181 aggtcgaagc ggctttcaac ttcaaacttg aaaacaatca tgccttcctt gctggtgccg
    75241 tttcacgtaa gaaacaagtg gtacctcaat tgactgaaag ctttaatgcg taagattttg
    75301 ggtgtcagct caaaatcgga aagtctagtt tgccttatat cgaaaggagt ttcggctcct
    75361 tttttctagg agtgaagtat gttagaaaat ggcgatttga tttttgtgag agatgggtca
    75421 gacatgggac aggccatcca gacttccaca ggtaactata gccatgttgc catttatttg
    75481 gatgggatga tttatcatgc tagtggacag gctggtgttg tctgtcaaga accggcagac
    75541 ttctttgagt ccaatcattt atacgacctc tatgtttacc cagaaatgga tatccagtcg
    75601 gtgaaggaaa gagcttgcaa acatcttgga gcaccctaca atgcttcttt ctatccagat
    75661 gcagctggtt tctactgctc ccagtatata gcagaaatcc tacctatttt tgaaactatt
    75721 cctatgaaat ttggagatgg ggagcaggag attagtgatt tttggaggga gtattacata
    75781 gaactaggtc tgcctgttcc tctgaaccaa gctggtacca atcctagtca gttggcagca
    75841 tcgcctctgt tacaatgtaa agaaagggat cttcatgatt cagattttta atccatctcg
    75901 tttgacgaga cagccatttt tggagaattg atccgctatc tggaccagta tgaggatgtg
    75961 attctacggg aaattaaggc tcaatttcca gatgttgcag ttgataaact catggaagag
    76021 tatataaagg caggcttgat tctacgtgaa aataagcgct attacctcaa ttttcctacg
    76081 cttgaatcac ttgatagtct tgaactggat caagagattt ttgtcagaga agctagtccg
    76141 gtctatcaag ccttgttgga gcagagtttt gagacggaat tgcgcaatca aatcaatgca
    76201 gctattttag ttgaaaagac ggactttgcg cgcattaaaa tgaccctgtc caattatttt
    76261 tacaaggtca aacagcagta tcctttgaca gaaaaacagc aggagctcta tgacatttta
    76321 ggagatgtta atcctgagta tgccctcaag tatatgacgg cttttttgtt gaaatttctc
    76381 aaaaaagacc agcttatgca gaaatgccgt gatatctttg tggacagttt ggttgtctta
    76441 ggctatattg tgcaaaatga agatagaaag tatgagttgg ctatcgattt tgataaggag
    76501 agattaactt tctacttagc gtgatttctt gtttctgagc acattgtttg actttcctta
    76561 gtattcggta taaactatat gtaaccggta acacatatcg gaataaacta aaggagacaa
    76621 tcatatgtca cttgaaaaca aattggaaca agcaacaggc gctgtcaaag aaggttttgg
    76681 taaagttact ggagacagca agacagaact tgaaggagct gttgaaaaaa cagttgctaa
    76741 ggcaaaagac gttgtagaag acgcaaaagg tgctgtagaa ggtgccgttg aaggtttgaa
    76801 aaacgttttt actaaataat aggaaaaaat caagggtttc attttccctt gattttttct
    76861 attcttataa ataattttct gcgacggctg tatctcctgg gtaggattct ttcttgccct
    76921 ggatgatttg gtaacaatcg actcccttac ccgcaataat aactgcatct aattcgtgat
    76981 ttgtgatagc cattgccgcc ttgatggctt cttggcgatc cgcaatcttt tcaacaggat
    77041 gattgatgta gctactaatt tcatctgcaa tggccattgg gtcttcatag ttagggtcat
    77101 cagcagtcag aaagacttga atctcagggt gttgattgag gaggaggcca aagtccttac
    77161 gacgactttc tcccttgttt cctgttgatc ccagaaccag agcaatcttt ccggtttgat
    77221 gagtttcaac cacattgatg agttttttca gactatcccc attgtgggca tagtcgatga
    77281 agaccttggc tccatttttc tgagtgagga cttccatacg accaggaacg cgggttgcag
    77341 cgatgccttt tttgatgtcc tcaagacttg ctccgagacg gagacaagca agtccagcag
    77401 caactgcatt ttcttggttg aagttgccaa tgagttgaat atcataatct ccagcgagtt
    77461 tacccgtagc tgaaaagcta aaggctttgg aattctcgat ttggttatca aattggctac
    77521 catagaaatc atggtcttga tcttcaacct gttctttcaa gactgagaag tggtccatgt
    77581 cactgttaat gatgactgct cggctctttt ccatcaagag acgcttgtgg tagaaatagt
    77641 cttcaaagct agggtgttca atcgggccga tatggtctgg gctgatattt aggaaaactc
    77701 ccacatcaaa ggttagacca tagacacgtt tgaccagata ggcttgactg gagacttcca
    77761 tgatgaggtg ggtacggtca ttttgcacag cctgattcat catgtcaaag aggtcaatac
    77821 tctcaggggt tgtcaacgct gacttaaaga aagtctcgcc atcaagagtt gtgttcatgg
    77881 tcgacaacat agcaggtcta tgcccttgag ataagatgtt ataggcgaaa taggctgctg
    77941 ttgtcttacc cttagtacca gtaaaggcaa ggagtttgag tttttcctgt ggattaccat
    78001 agaactccat ggcaatcaaa ctcatggctt tctttatatc gttcacaatg atgacaggga
    78061 taccgacttc gtagtccttt tcagctacat accaagctaa tccttgtgtt atagcagaaa
    78121 gaaggtattc ttttttaaag gcagcgcctt ttgcgaaaaa aagagtgtct tctgttactt
    78181 ttcggctgtc gtagctgatg ctatcaaaaa taactttgct gtagttgtag tggtaatgac
    78241 cttggtcaat aatttcgcga aaaaggccat ctttctttaa aatatctaat acggtttcaa
    78301 tcttaatcat actttctatt gtaaaccgaa agtcgtaaat ttacaagtaa caaggaaaag
    78361 tttataatgg aagataagga gtttttccta gctatcaaaa ttgaatgagg aatctatgtc
    78421 gcacgaaaac aatcaccagc aggcccagat gttacggggg actgcttggc taacggctag
    78481 taactttatc agtcgcctac tcggggctgt ttacattatc ccttggtaca tctggatggg
    78541 ggcttatgca gctaaggcaa atggtctctt taccatgggt tacaatatct atgcttggtt
    78601 cttgttggtt tcaacagcgg ggattccagt tgcggtggcc aagcaagttg ccaagtataa
    78661 taccatgcga gaagaagagc atagctttgc cctgattcgg agtttcttag gctttatgac
    78721 aggactaggc ctggtttttg ctttagtctt gtatgtcttt gctccttggc tagcagactt
    78781 gtctggcgtg ggcaaagact tgatcccaat catgcaaagc ttggcttggg gagtcttgat
    78841 tttcccgtct atgagtgtta tccgaggatt tttccaaggg atgaataacc tcaaacccta
    78901 tgccatgagc caaattgctg agcaggtcat tcgtgttatc tggatgctcc tagcaacctt
    78961 tatcattatg aagctcggtt caggagatta tctagcagcc gttacccaat caacctttgc
    79021 tgcctttgtc ggtatggtag ccagttttgc agtcttgatt tatttccttg cccaagaagg
    79081 ttcactcaaa agagtctttg aaacaggaga taagattaac agtaagcgtc tcttggttga
    79141 taccattaag gaagccattc cttttatcct gacagggtct gccatccagc tcttccagat
    79201 tttggatcag ctgaccttta tcaatagtat gagctggttt accaactaca gcaatgagga
    79261 cttggttgtc atgttttctt atttctcagc caatcctaat aaaatcacga tgattttgat
    79321 ttctgtaggg gtttcgattg ggagtgttgg tttgccactt ttgacggaaa actatgtcaa
    79381 gggggacttg aaagcagctt ctcgtctcgt tcaggacagt ctcaccctac tctttatgtt
    79441 cttgctacca gcaacggttg gagtggttat ggtaggagaa cctctatata cagtcttcta
    79501 tggtaagcca gatagtttgg ctctgggctt atttgtcttt gcagttttgc agtctattat
    79561 tttaggcttg tacatggtct tgtctccaat gcttcaggcc atgttccgca accgcaaggc
    79621 cgttctctat tttatctatg gttctattgc caagctagtc ttgcaactac ctaccatcgc
    79681 cctcttccac agttatggtc ctttgatttc aacaaccatt gctctcatca ttcctaacgt
    79741 cttgatgtat cgggatattt gtaaagtaac tggtgtcaag cgcaaggtga ttttgaagcg
    79801 aactatttta atcagtttgc tgaccctagt catgtttctg ttaataggaa ccatccagtg
    79861 gctgttagga tttttcttcc aaccaagtgg acgtttgtgg agcttctttt atgtagctct
    79921 tgtcggtgcc atggggggtg gactttatat ggttatgagt ctgcgtacct atttattaga
    79981 taaggtaata ggaaaagccc aagcagatcg cctgcgagca aaatttaagc tttcgtaagg
    80041 tagcatttat aaataaaagg aataagttga acaatctttt aggagaactt gaaacttatt
    80101 ccttttttag cgtaaaaata gctaaaatta tctttcaaaa atagaaaatg aggttttttt
    80161 gattttttgg aaaaaacaat tgactaaaca aaccaagagt tgtataataa aacaaatatt
    80221 taaagcagga ggttccggaa atgaaaaagt ctaagagcaa atatctaacc ttggcaggtc
    80281 ttgtcctggg tacaggagtt ttattgagcg cgtgtggaaa ttctagcacg gcgtcaaaaa
    80341 cctacaacta tgtttattca agtgatccat ctagcttgaa ctatctagca gaaaaccgcg
    80401 cagcaacatc cgatattgtt gcaaatttgg tagacgggtt attagaaaat gaccaatatg
    80461 ggaatattat tccatcatta gcagaggatt ggactgtttc tcaggacggt ttgacctata
    80521 cctacaaact tcgtaaagat gccaagtggt ttacttctga gggagaagaa tatgcgcctg
    80581 taactgccca ggattttgtg acaggtttgc aatatgcagc tgataaaaaa tcagaagcct
    80641 tgtatctagt gcaggactct gttgctggtt tggatgacta tatcactggt aaaacaagcg
    80701 acttttcaac tgtcggtgtc aaggcacttg atgaccaaac ggttcaatat actttggtta
    80761 aaccagaact ttactggaat tcaaaaacac ttgcaacgat actttttcct gttaatgcag
    80821 atttcctgaa atcaaaaggg gatgattttg ggaaggcgga tccatctagt attttgtaca
    80881 atggaccttt cttgatgaaa gcacttgtct caaaatctgc tattgaatat aagaaaaacc
    80941 ctaattactg ggatgctaag aatgtctttg tagacgatgt gaaattgacc tactatgatg
    81001 gtagcgacca agaatcactg gaacgtaatt ttacagctgg tgcttatact acggctcgtc
    81061 tttttcctaa cagctccagc tatgaaggga ttaaagaaaa atacaaaaac aatatcatct
    81121 atagtatgca aaattcaact tcatatttct ttaattttaa cctagatagg aagtcttaca
    81181 attatacttc taaaacaagt gacattgaaa agaaatcgac tcaggaagca gttctcaata
    81241 aaaacttccg tcaggctatc aattttgctt ttgacagaac atcttatggg gctcagtctg
    81301 aagggaaaga aggtgcaaca aagattttgc gtaacctagt ggttcctcca aactttgtca
    81361 gtatcaaggg aaaagacttt ggtgaagttg tagcctctaa gatggtcaac tatggtaagg
    81421 aatggcaagg tatcaacttt gcggatggtc aagaccctta ctacaatcct gagaaagcca
    81481 aggctaagtt tgcggaagct aagaaagaac tcgaagcaaa gggtgttcaa ttcccaatcc
    81541 acttggataa gactgtggaa gtaacagata aagtaggcat acaaggagtt agttctatca
    81601 aacaatcaat tgaatctgtt ttaggttctg ataatgtagt gattgacatt cagcaattaa
    81661 catcagatga gtttgacagt tcaggctact ttgctcaaac agctgctcag aaagactatg
    81721 atttatatca tggcggttgg ggacctgatt atcaagaccc gtcaacctat ctcgatattt
    81781 ttaatactaa tagtggagga gttctacaaa atcttggact agagcctgga gaagccaatg
    81841 acaaggctaa ggcagttgga ctggatgtct atactcaaat gttggaagaa gctaataaag
    81901 agcaagatcc agccaaacgt tatgagaaat atgctgatat tcaagcttgg ttgattgata
    81961 gttctttagt tcttccaagt gtttcgcgtg ggggaacacc atcattgaga agaaccgtac
    82021 catttgctgc tgcctatggt ttaaccggta caaaaggggt tgaatcatat aaatacctca
    82081 aagtacaaga taagattgtc acaacagacg aatatgcaaa agccagagaa aaatggttga
    82141 aagaaaaaga agaatccaat aaaaaagccc aagaagaatt ggcaaaacat gtcaaataaa
    82201 atctaattgt agataagttt gtgtaagaag tctttgtgct taaaaacgca taatatcagg
    82261 tatataaaat cctagatgtt atgcgttttg ttataggaag atttattaga ttttatcctc
    82321 aaattgagtt attctcgact ttatccgttg aaatattata aggagtattt atgaaattaa
    82381 aattattaag agtagatact aaggtgatta tggggagttt cttacttgtt ctgtctagtc
    82441 tacttgcttt gttgcttccc cttatcttaa agggtttaat agatgggagt tctattgaaa
    82501 atataggttc caaagtattt caatcgtttt tgatttttat tggtcaagcc ttgttttctt
    82561 ctattggtta ctatctgttt agtcaatcgg gtgaaaaaaa gatagcaaaa atcaggaaaa
    82621 aagtgataga ggggttgatt tatgcagaga aatccttctt tgataagagc caaagtgggg
    82681 agttgacttc tgccattgtc aatgacacga gtgtcattcg tgagttttta attacgactt
    82741 tcccaaatat tattctgagt ttagttatgg tacttggttc cattgtagtc ttatttagtc
    82801 ttgattggaa tctttctcta cttttattca tcactcttcc ttgtatgatg tttattatct
    82861 tgcccctttc caatatcagt gaaaagtata gtcgtcgttt acaggaggaa atcggatttt
    82921 taacagggca attgactgaa aagattcaag aacatgaatt aattaagact aaccaagcag
    82981 agaaaagtgt acaggatgtt ttggataatt gtattgaaag ggtacaaaac aattcactga
    83041 aatcagatag ggttacttct tttgagaccc cgtttgctct cttatttatc tttgcgacta
    83101 tagttgtgat gctaacctat gggggttatc gggtcagcgc aggatatata tctgtgggaa
    83161 ccttggtttc gtttttgatt tacctctttc aattacttaa tcctattagt aatatagcta
    83221 attttgtaac tatttattct aggagcaagg gatcttcagt tgcactggag aacttgcttg
    83281 cagttcctaa agaaaaattt gagtgaggaa aatcggtatc aggacaaggg ttgaatttta
    83341 accatgtcta ttttggttat gatgaaaatc gacctgtctt aaaggatatt acttgttcaa
    83401 ttttcaaggg gcaaaaaatt gcttttgttg gaccatctgg atcaggaaaa tcaacgattg
    83461 tgcgtttgtt agagcggttt tataaaccgc tttcaggata tattctaatg gagcaatcaa
    83521 gtatatatga ttttaactta aaagaatgga gaagtaaaat cgcttgggtt tcacaaaata
    83581 atgcagtctt atctggcagt attcgtgaca atctttgtct cggtttgaat cgcttagtaa
    83641 ctgatgatga attgatgaaa gtgctagact tagtatcact aggtgatgag attcgctcca
    83701 tgaaagaggg actagatact gaagttggtg aacgcggacg attcttgtca ggggggcaaa
    83761 gccaaagact tcaaatagct agaacctact taaaagatgc tgaaattctt atatttgatg
    83821 aatccactgc taatcttgat gcggattctg agtatgcgat tatcagtatc ctctattctg
    83881 cattaaagga gaagacggtt gtgattatag cgcatcgttt gtcaacggta aaagatgtgg
    83941 attgtatttt cttcttagag ggggggaaaa tcactggctc aggaactcat aaggaactac
    84001 tggaaaatca tgagcgttat gctcgttttg tgcaggagca aatgatagag tgaaggtgtc
    84061 ttttgagatt caccatttta tagtctatta aagggagcag gaaaaactcc ctttttatat
    84121 agtttgaaac tataactagc tcttgaaaag aagaaaatga gttgatgaaa ataagtggta
    84181 caatagttac tatagatttg gaggtattgt atgagcaagg aattacacat taacacaatt
    84241 ttggctcagg cgggtattaa gtcagatgaa gcgacaggtg cattggtgac accgcttcat
    84301 ttttcaacga cctatcagca tccagagttt ggtcgatcta ctgggtttga ctatacgcgc
    84361 actaaaaatc caactcgtag taaggctgag gaagtcttgg cggctattga gtcagcagac
    84421 tatgccctag cgactagctc agggatgtca gctattgtac tggcctttag cggctttcca
    84481 gtaggaagta aggtcttggc agtgcgtgat ctttacggtg gttcttttcg ctggtttaac
    84541 caagtggagc aggaaggtcg tttccatttt acctatgcca atacagaaga agagttgatt
    84601 gctgagttag aaaaggatgt ggatgttctc tatatcgaaa ccccaaccaa tcccttgatg
    84661 ttggaatttg atatcgaaaa actagcaaaa ttggctcatg ctaagggtgc caaagtggtg
    84721 gtggacaata ccttctatag ccctatctac caacgtccga ttgaagatgg agcagatatc
    84781 gttctccatt cagcaaccaa gtatctagca ggccacaatg atgtcttggc tggagtggtt
    84841 gtgaccaata gtttagaact atacgagaag cttttttaca atctcaatac aacaggggca
    84901 gtcttgtctc catttgacag ctaccagttg cttcgtggtc tcaagacctt gtctcttcgt
    84961 atggagcgct caacagctaa cgcccaagaa gtggttgcct ttttgaagga ttctccagca
    85021 gttaaggaag ttctctacac tggtcgtgga ggcatgattt cctttaaagt agccgatgaa
    85081 acacgcattc ctcatatttt gaacagtctc aaggtcttct cttttgcgga aagtttgggc
    85141 ggagtggaaa gtcttattac ttatccaacg actcaaactc atgctgatat tccagcagaa
    85201 gtacgccatt cttatggttt gacagatgac ctcttgcgtt tgtctattgg gattgaggat
    85261 actagagatt tgattgcaga tttgcgccaa gccttagaag gataagacaa agatgggaaa
    85321 atatgatttt acaagcctgc ccaaccgttt agggcaccat acctataaat ggaaagaaac
    85381 agaaacggat agtgaagttc taccagcttg gatagcggat atggactttg tggtcttgcc
    85441 tgaaatccgc caagccgtgc aaacttacgc agaccaactg gtttatggtt atacctatgc
    85501 cagtgaagac ttaattaagg aagttcaaaa gtgggaagct acacaatacg gttacaactt
    85561 tgacaaagag gctcttgtct ttatcgaggg tgtggtacca gccatctcaa cagctattca
    85621 aacctttaca aaagaaggcg aggcggtttt aattaacacg cctgtctacc caccctttgc
    85681 tcgcagtgtc aagttgaata atcgtagatt gattactaat tccttagtgg aaaaggatgg
    85741 tctgtttgag attgactttg accaacttga aaaggatttg gtggaagagg aggttaaact
    85801 ctatattctt tgcaatcctc acaatcctgg tggacgtgtt tgggaaaaag aagtgttgga
    85861 gaagattggc caactctgcc aaaaacacgg tgttttgtta gtttcggatg agattcacca
    85921 agatttgacc ctctttggtc acaaacacca gtctttcaat accatcaatc ctgccttcaa
    85981 aaattttgct atcgtcttga gcagtgccac taaaacattt aatattgctg gaacaaaaaa
    86041 ttcctatgca gtcattgaaa atcctaagtt gagactggct ttccagaaac gcctgttggc
    86101 caataatcag catgaaattt caggcttggg ttatttggcg acagaagctg cctatcgata
    86161 cggtaaagat tggctagagg aactcaagca agtctttgaa gaccacatca attatgtggt
    86221 agatctattt ggaaaagaga ctaaaatcaa ggtcatgaaa ccgcaaggta cctacttgat
    86281 ttggcttgac ttttcagcct atgacctgac tgatgaaaca ttgcaagagt tgttgagaaa
    86341 tgaagccaag gttatcctca accgtggttt ggattttgga gaggaaggaa gtctccatgc
    86401 ccgcatcaat atagctatgc ccaaatctct gttgcaggaa gtctgtcagc ggattgtggt
    86461 tacttttgcc aaacgttaaa aatccagtct tctaggagaa aagtcttcct agaaggctat
    86521 tttcataggc gaaaatatgg tataataaaa agataaggta aaggtgaaaa tatggctaaa
    86581 ttgattccgg ggaaagttcg tatcgaaggt gttgcccttt atgaaactgg taaggttgat
    86641 atcatcaagg aaaagaacaa tcggctctac gctcgcgttg cagaagaaga actgcgctat
    86701 agtttagagg atgatttggt tttttgtgcc tgtgattctt ttcaaaagag gggctactgt
    86761 gtgcatttgg cagcgctaga gcattttctg aaaaatgatg agcgtggtca ggaaatcttg
    86821 tggagtctgg aagaaggtca tgaagaaaaa gaggccgttg aaaccaaggt gaccttgggt
    86881 ggcaagtttt tgaatcgaat tttatctcca aaatcagaat gcgcctatga gttatcagct
    86941 gtgggtcagg tggaagcagg aaccaatcat atcttgtgga ctctgagaat cggccaaatc
    87001 aatagccaaa aatactatgt cattcgggat attccacttt ttttaaggat tgtcgagcag
    87061 agaaagtcgt atatgattgg caagatttat gaggagtctc tttcttggga agcctttgat
    87121 gatgccagcc aagaactttt gactttcttg cacggactaa cggaggaagg gctggttcca
    87181 gacctctttt tccaaaacca aggtcggcac ctctttttcc ctctgacctt ttttgagcag
    87241 ggtgtgaatt tactgatggc cttgccccat tttcaatttg accatcaagt ggatagctac
    87301 caaaccctgc tttttcagga tatgcatgct gatgccaatc tatttgcctt tacagtaaca
    87361 gaatactcgg actattttga aatggaaatc agtgagagtc ctagggtcaa tgtcttttat
    87421 caaggagcag tacttttcca taaaggtcag gtttattttt taacagacca gcagatgcgt
    87481 cttctcaagg aaattaaagc gttacctgtg ggtcaacaag gaaagaaata cctgcaattt
    87541 gatagcagtg atcgagacaa attggcctcc tgtctgactc tttttatcca gatgggcacc
    87601 gtttcagctc cagaacgtct acaaatcaaa acttttgcgc cctcttttta ctttgacagg
    87661 gaagaagata atcggattcg tttagaaatt cagtttgatt atggaaatag acaggtatct
    87721 agccgacaag agctagaaga attacccttt tcaagtgacg cggacttaga agaaagaatt
    87781 ttccaagtct gtttggctgc gggctttgaa gccgattttc aatcttggcg tcaggcctta
    87841 aaagcggagt ctgtctatca tttttttcat gatatcattc cagtctttga aaaactcggg
    87901 catgttgacc tatcagataa actagaagag ctttatagcc tagcgagtcc tcaggtgcag
    87961 attgcctcca agggaggtct cttggaaatc cagtttgatt ttcaaaatat tgcccaggag
    88021 gaaatcgacc aagccatgca ggccttggtt gccaatcagg atttttatat tgattcgtct
    88081 aatcaagttt actttttcga tgaagaaacc aagaaaattc gccaaaatct ccaggaactg
    88141 gggcaatttg aattaaaaga tggaaccttg caggctcgga aatccttggc ctacagttta
    88201 gctcatctct ttgaagggcg agaccgtgtt tctttttcac aagaattcca gaatctggcc
    88261 caggatttga cgcatccaga ggactttcct ttgcaagcaa ctcaggtcaa ggctgacttg
    88321 cgagattatc aggaaaaggg aattggctgg ttgcagatgc tccatcatta tggttttggt
    88381 gggattttgg ctgatgatat gggacttggg aaaacccttc agaccattgc ttttttgact
    88441 agtcaagtga caaaagaaag tcgagtcttg attctcgctc cgtcaggttt gatttacaac
    88501 tgggcagatg agtttcagaa atttgcccca cagttggatg tggttgttgt tcatggtttg
    88561 aaagcaagtc gtgaggagat tcttgccgag agccatcaaa tctatgtgac tagctatgcc
    88621 accttccgtc aggacagtga gttttatcag gggatggcct ttgacttcct tttcttagat
    88681 gaggctcagg tcatgaaaaa tgcccagacc aagattgccc agaccttgag acaatttgtg
    88741 gtgccgtcgg tctttgcctt gtcaggaact ccgattgaaa accatctggg tgagttgtgg
    88801 tctattttcc aaatcgtcat gcccggactt ttgccaagca agaaagaatt tatgaaattg
    88861 ccagcagagc gagtggctca gtttatcaag cctttcgtga tgcgacgcaa gaaagaagaa
    88921 gttctgactg aattgccaga cttgattgag gtggtttata aaaatgaact ggaagaccag
    88981 caaaaggcta tttaccttgc ccagttacaa cagatgcgag accatctggc tcaagtgtca
    89041 gaacaggaat ttcagcgaag tcgtgtggaa attttatctg gtttgatgcg cttgcgtcaa
    89101 atatgtgaca ctcctgccct gtttatggaa gattatcagg gagccagcgg caaactagat
    89161 agtctccgag acctactggt acaggtggca gacggcggac accgtgtctt gattttctct
    89221 cagttcaagg gaatgttgga aaaaattgaa caagaactgc cagacttggg cctgacttcc
    89281 tttaaaatta cgggttcaac cccagccaag gaaagacaag acatgaccaa ggcctttaat
    89341 caaggagaaa gagatgcctt tctgatttcc ctcaaggctg gtggggtcgg tctgaacctg
    89401 acaggtgcag atacagtgat cttggttgac ctttggtgga atcctgcggt ggaagcgcaa
    89461 gccattggcc gtgcccatcg gatgggtcag gaagaaacgg ttgaggtcta tcgcttggtg
    89521 accaagggaa ccattgaaga aaaaattcag gaactccaag aacaaaagaa acatctggtg
    89581 tcacaagtat tggacggcac agagtcacgt ggtagtctga ccctagcaga aattagagaa
    89641 attttgggaa tttctgaagc cagcacttga aaaatcaaga caatacgcta taatgaagtg
    89701 ttgaaaggaa atagaaaatg aaacaagaac gatttccatt ggtgtcagat gacgaggtca
    89761 tgttgactga aatgccagtc atgaatctct atgatgagtc tgatctgatc agtaatatca
    89821 agggtgagta tcgagataaa aattatttag aatgggctcc tattgctgaa gaaaaaccag
    89881 tacaaccgat tgaaaagcaa gtagggaaac ctaaaaaagc tcctttaggg gttaaaaaag
    89941 aaggaaagag ctatgcggag gtggcgcgtg aagaagcgcg tgcagacttg aaaaagaaac
    90001 gctctgctaa ctatctaact caggatttca gccttgcgag acgtcattct cagcccagtc
    90061 tagttagaca gggcaatcaa ccgacaactc ctttccaaaa ggaaaatcct ggtgaatttg
    90121 tcaaatatag ccaaaaattg acccagtctc attatatctt ggcggaagaa gttcattcta
    90181 tccctaccaa gaatgaagaa gtgtcagcac ctgctccaaa gaaaaacaat tatgattttc
    90241 taaagaagag ccaaatctac aataaaaaaa gtaaacaaac agaacaagaa cgtcgggttg
    90301 cccaagagtt gaatctgacc agaatgacag aataggggag aaaacatgtc aaagacatat
    90361 cattttatcg gaattaaggg atcagggatg agtgccttgg ccttgatgtt gcaccagatg
    90421 gggcacaagg ttcagggatc agatgttgaa aagtactact ttacccaacg cggtcttgag
    90481 caggcaggaa ttaccattct tccttttgat gaaaagaatc tagacggtga tatggaaatt
    90541 atcgctggaa atgcctttcg tccagataac aacgtcgaaa ttgcctatgc ggaccaaaat
    90601 ggtatcagct acaaacgtta ccatgagttt ctaggtagct ttatgcgtga ctttgttagc
    90661 atgggagtag caggagcaca tggaaaaact tcaacgacag gtatgttgtc tcatgtcttg
    90721 tctcacatta cagataccag cttcttgatt ggagatggga caggtcgtgg ttcggccaat
    90781 gccaaatatt ttgtctttga atctgacgaa tatgagcgtc acttcatgcc ttaccaccca
    90841 gaatactcta ttatcaccaa cattgacttt gaccatccag attatttcac aagtctcgag
    90901 gatgttttta atgcctttaa cgactatgcc aaacaaatca ccaagggtct ttttgtctat
    90961 ggtgaagatg ctgaattgcg taagattacg tctgatgcac caatttatta ttatggtttt
    91021 gaagttgaag gcaatgactt tgtagctagt gatcttcttc gttcaataac tggttcaacc
    91081 ttcaccgttc atttccgtgg acaaaacttg gggcaattcc acattccaac ctttggtcgt
    91141 cacaatatca tgaatgcgac agccgttatt ggtcttcttt acacagcagg atttgatttg
    91201 aacttggtgc gtgagcactt gaaaacattt gctggtgtta aacgtcgttt cactgagaaa
    91261 attgtcaatg atacagtgat tatcgatgac tttgcccacc atccaacaga aattattgcg
    91321 accttggatg cggctcgtca gaaataccca agcaaggaaa ttgtagcagt ctttcaaccg
    91381 cataccttta caagaaccat tgccttgttg gacgactttg cccatgcttt aaaccaagca
    91441 gatgctgttt atctagcgca aatttatggc tcggctcgtg aagtagatca tggtgacgtt
    91501 aaggtagaag atctagccaa caaaatcaac aaaaaacacc aagtgattac tgttgaaaat
    91561 gtttctccgc tcctagacca tgacaatgct gtttacgtct ttatgggagc aggagacatc
    91621 caaacctatg aatactcatt tgagcgtctc ttgtctaact tgacaagcaa tgttcaatag
    91681 gatgttccca tggaaattcc aattaagatc attcaggcaa gcaagtctga tttgcctgag
    91741 ataggggcac ttcagacctc gtcttttcca gctgaaaagc agcaactttc ccatatttta
    91801 gaagaaagta tccgtaagtg tgcggatacc tttcttctag ctagggatga aaatcaactt
    91861 ttaggctata ttttatcaag tccccagtca gacaatccgc aatatctaaa agtacattct
    91921 ttagtcatcg agtctgacca tcagagacag ggcttgggaa cacttcttct tgcagccttg
    91981 aaagaggtgg cagttgagct ggattacaaa gggattcgtt tggagagtcc tgatgagctg
    92041 ctttcctatt ttgaaatgaa cggttttgtt gatgaagaag caactttgct ctatgcaact
    92101 agccagggct atagtatgat ttggtttaat cccttttatc tggaggaaca atgaaaatca
    92161 gacaagcaag attagaagat ttggatcgga ttgttgaact tgaatttgaa aatttttcgg
    92221 tcgaagaagc cattcctcct tctgtctttg aagcacattt gagagaaatt cagacctctt
    92281 ttctggttgc tgaaaaagaa ggaagaatca tgggttatat cgaaggacca gttggcctgc
    92341 accgccatct gcaagaccag tcttttacag aagaaataaa agactatagt catgagcctg
    92401 gtggttatat ctctgtgacc tgtctgtcta ttgccaagga ggcacaggga ctcggactgg
    92461 gtcagaaatt gctgacagcc ttgaaagaag ttgctcttga agatgagaga gatggcatta
    92521 atctaacctg tcatgactat ctcatcgcct attatgaaaa acatggattt gtcaacgaag
    92581 gccagtccca gtcaaccttt gcaggggaaa catggtatga tatggtctgg gaaatgaaaa
    92641 aataagttag gaaaagtatc atagattgct ttttttggac ttttaagata taatattatg
    92701 gattatcaac aaggaggaaa aacttttgag tgaaaagtca agagaagaag agaaattaag
    92761 ctttaaagag cagattctga gagatttaga aaaagtaaaa ggctatgatg aagttctgaa
    92821 agaagatgag gcagtagttc gcactcctgc aaatgaacct tcagctgaag aactcatggc
    92881 tgattccttg tcaacggtag aggagattat gagaaaagct cctaccgtgc ctactcaccc
    92941 aagtcaaggt gtaccagctt ctccagcaga tgagattcaa agagaaactc ctggtgttcc
    93001 aagtcatcca agtcaagatg taccttcttc tccagcggaa gaaagtggat caagaccagg
    93061 tccaggtcct gttagaccta agaaacttga aagagaatac aatgaaaccc caacaagggt
    93121 agctgtttcc tatacgacgg cagagaaaaa agcagaacaa gcaggttcag aaacacctac
    93181 gcctgctaca gaaacagtgg atatcatcag cgatacatca cgtcgtagcc gtagagaagg
    93241 agcaaaaccc gttaagccta agaaagagaa gaagtcacat gtgaaagctt ttgtgatttc
    93301 attccttgta ttccttgcct tgctctcagc aggtggttac tttggttacc agtacgtgct
    93361 agattctttg ttacctatcg atgctaattc taagaaatat gtgacggttg gaattccaga
    93421 aggttcaaac gttcaagaaa tcggtacgac gcttgaaaaa gctggtttgg taaagcatgg
    93481 tctgattttt agtttttatg ccaagtataa aaattatacc gacttgaaag caggttacta
    93541 caatttgcaa aagagtatga gtacagaaga cttactcaaa gagttgcaaa aaggtggaac
    93601 agatgaaccg caagaacctg tacttgcgac tttgacaatt ccagaaggtt ataccttgga
    93661 tcagattgct caagctgtgg gtcaattgca aggtgacttc aaagagtctt tgacagcgga
    93721 ggctttcttg gctaaagttc aagatgagac gtttatcagt caagcagtag cgaaatatcc
    93781 tactttactg gaaagtttgc ctgtaaaaga cagcggtgcg cgttatcgtt tggaaggata
    93841 ccttttccca gctacatact ctatcaagga aagcacaact attgagagct tgattgatga
    93901 gatgttagct gctatggata agaacctatc tccttactat agtactatca aatctaaaaa
    93961 tttgactgtc aatgagttgt tgaccattgc ttccttggtc gaaaaagaag gtgccaagac
    94021 agaagatcgt aagctcattg caggtgtatt ctacaatcgt ttgaatcgtg atatgccact
    94081 tcaaagtaat attgcaatct tgtatgccca aggaaaactg gggcaaaata tcagtctagc
    94141 tgaggatgtt gcgattgata ccaacattga ttcaccttat aatgtttata aaaatgtagg
    94201 tctcatgcct ggtccagtcg atagtccaag tctggatgcg attgagtcaa gcatcaatca
    94261 aactaagagc gataacctct actttgtagc agatgtcaca gaaggcaagg tctactatgc
    94321 taacaatcaa gaagaccacg accgcaatgt cgctgaacat gtcaacagca aattaaacta
    94381 aacaaactaa aattatgtga tacttcacat aattttcttt agaaaatatt atcagaagaa
    94441 agttgagaaa aatggcagaa aaaacatatc ctatgaccct tgaggaaaag gaaaaacttg
    94501 aaaaagaatt agaagaattg aaattggttc gtcgaccaga agtggtagaa cgcattaaga
    94561 ttgcccgttc atacggtgac ctttcagaaa acagtgagta cgaagcagct aaggatgaac
    94621 aagcctttgt cgaaggacaa atctctagct tagaaacaaa aatccgctat gctgaaatcg
    94681 tcaatagcga cgcagttgcc caggacgaag tagcgattgg taaaacagtc accatccaag
    94741 aaattggtga ggacgaagaa gaagtttata ttatcgtagg ttcagctggt gcggatgcct
    94801 ttgcaggtaa ggtttcaaat gaaagcccaa ttgggcaggc cttgattggc aagaaaacag
    94861 gtgatacagc aaccattgaa acgcctgttg gtagctatga tgtaaaaatc ttgaaggttg
    94921 aaaaaacagc ctaaaaacag aaaaaggagt ggggaggcga tgtgcttcac tcactccttt
    94981 ttccattttg ctactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttgccgtatg
    95041 tatggttact gacttcgtca gtttcctagt ttgctctttg attttcattg agtattagat
    95101 gtataatgca ttgaagttaa aatagtacac tttgatttct aagacattgt tagaaattag
    95161 attaaattcc ttaatcgatt tgcgcatgtt ttatttcatt tcattatacc ttagattgga
    95221 aaataaggaa tcacataaat cgcaagaaat agggaatctg attcgtgttt ataaccgttc
    95281 aaaaagagaa gaggctgaaa gtgagccact taatctttat gtcgaagatg aaaagggcaa
    95341 tctccttgcg ggtttgatag cagagacttt tggaaattgg ctagaaatcg agtatttgtt
    95401 tgtaaaagag gaactgcgag ggcaaggaat cggttcaaaa ctattgcaac aggcagaaag
    95461 tgaagctaag aatcgaaatt gttgttttgc ctttgtcaat acttaccagt tccaggcact
    95521 ggacttttat cagaaacatg gctacaagga agttttttct ttgcaagact atctctacat
    95581 taggcaaaga tattattacc aaaagaatct gtaaggcgag tatggaggtg gactgatgta
    95641 tatgagtaac tatcgataaa taccagttat aacctagaaa ttgtgtcttt ctagacgata
    95701 ttgaggacaa tataatcgta gccccaaaat taggaatcaa ggcttatcag gttaagaaaa
    95761 gaagtgatgt tgttgatatt ttaaaacaat ttatttaaac taaaaactct ctatcttttt
    95821 aaattgacat taatccgaga ttcccctctc ggattttgtg ttgctttcat taaatttaca
    95881 ggtaagcctt gctagcttca gtaaatatac acaagttttt cagaatagag tgaaatccaa
    95941 aagaaaatgg aaataatgaa agctcttgta ggcataagaa aacacctctg tgttatactt
    96001 gttgttcaac cacaaacaca agaaaggcac agagatgcaa aaccattata ctacaaaagg
    96061 taaacatttg acaatcgata gccgtcgctt aatcgaacga tggaaaaaag aaggaaaatc
    96121 aaatagagaa attgcctctc tgcttggaaa agttcctcaa actatccaca ctgaaatcaa
    96181 gcgtgggaca gtccgacaat gtcttggaaa agggcgcttc aaagaggttt attctgccga
    96241 ctatgctcaa cagtcttatg aaaacaatcg caagcgctcg gtcaggaaat taagcgtgac
    96301 caaggaacta aaggaaaaga ttctccacta tcataaccaa aaattttcgc ctgaaatgat
    96361 ggtgatggct aaaggggtta acgtgggaat ttcaaccatt tactattgga ttcatcatgg
    96421 aaaattgggg ttaagcaaac aggatctgct ttaccctaga aaaggaaaag cgcttaagaa
    96481 acaggctagc atcaacttta aacctgctgg tcaatccatc gcacagcggc ctgaagctat
    96541 caatcttcgc ttggagaatg ggcattatga gattgatacg gttctactta cgagagcgaa
    96601 aaactactgc ttgcttgtct tgacggatcg aaagagtaga catcagatca tccgattgat
    96661 tccaaataaa agtgctgagg tggtcaatca ggctctaaaa ctcatcttaa aacaacacaa
    96721 gattctttcc atcacggcag ataatggaac ggaattcaat cgcttgtttg atatattttc
    96781 tgaggagcac atctattatg cgcaccccta tgcctcttgg gaaaggggaa ctaatgagaa
    96841 tcacaacagg ctcattcgta gatagttacc taagggaacc aagaaaatga ctcccaaaga
    96901 agtcgcattc atcgaaaagt ggattaacaa ctatcctaaa aaatgcttgg actacaagtc
    96961 acccagagaa gacttctgga tggctaactt gaacttgaaa tttagcaaaa tggaaataat
    97021 ttttattaaa cgcttccaat agcggttaaa agttgacaaa tttaaatttt aggactacac
    97081 taagtaagta aattttattt taaaggagaa tttgttatga atttaacatt tttaggctta
    97141 tgtattgcct gtatgggcgt atctgtcggt gaaggtttat tgatgaatgg actgtttaaa
    97201 tcagtagcac gccaaccaga tatgctttct gagtttcgta gtttgatgtt tttaggtgtt
    97261 gcctttattg aaggaacttt ctttgtaact cttgtcttct catttattat caaataaata
    97321 catggaacga gaagaaaagg gaggatttta gatggaagaa agtattaatc caatcatctc
    97381 tattggtcct gttatcttca atctgactat gttagccatg actttgttga ttgtgggagt
    97441 tatttttgtc tttatttatt gggcaagccg caatatgacc ttgaaaccca aaggaaagca
    97501 aaatatactt gagtatgtct atgactttgt tattggattt acagaaccta acattggttc
    97561 gcgctacatg aaagattact cactcttttt cctttgttta ttccttttca tggtgattgc
    97621 caataacctt ggcttaatga caaagcttca aacgatcgat gggactaact ggtggagttc
    97681 gccaaccgct aatttacagt atgacttaac cttatctttt cttgtcattt tgttgacaca
    97741 tatagaaagc gttcgtcgtc gtggatttaa aaaaagtata aaatctttta tgagtcctgt
    97801 ttttgtcata ccgatgaata tcttggaaga atttacaaac ttcttatctt tggctttgcg
    97861 gatttttggg aatatctttg caggagaggt catgacgagt ttgttacttc ttctttccca
    97921 ccaagctatt tattggtatc cagtagcctt tggagctaat ttggcttgga ctgcattttc
    97981 tgtctttatt tcctgcatcc aagcttatgt ttttactctt ttgacatctg tgtatttagg
    98041 gaataagatt aatattgaag aggaatagaa aggagtaact gatgcacgta acagtaggtg
    98101 aattaattgg taattttatt ttaatcactg gctcttttat tcttttgcta gtcttgatta
    98161 aaaaatttgc atggtctaat attacaggca ttttcgaaga aagagctgaa aaaattgctt
    98221 cagatattga tagagctgaa gaagcccgtc aaaaagcaga agtattggct caaaaacgcg
    98281 aagatgaatt ggctggtagc cgtaaagaag ctaagacaat cattgaaaat gcaaaggaaa
    98341 cagctgagca aagtaaggct aatatcttag cagatgctaa actagaagca ggacacttaa
    98401 aagaaaaagc caatcaagaa attgctcaaa ataaagtaga agctttacag agtgttaagg
    98461 gtgaggtcgc agatttgacc atcagcttag ctggtaaaat catctcacaa aaccttgaca
    98521 gtcatgccca taaagcactc attgatcagt atatcgatca gctaggagaa gcttaatgga
    98581 caagaaaaca gtaaaggtaa ttgaaaaata cagcatgcct tttgtccaat tggtacttga
    98641 aaaaggagaa gaagaccgta tcttttcaga cttgactcaa atcaagcaag ttgttgaaaa
    98701 aacaggtctg ccttcttttt taaaacaagt ggcagtagac gagtcggata aggaaaaaac
    98761 aattgctttt ttccaagatt ctgtgtcgcc tttattacaa aactttatcc aggttctggc
    98821 ctacaatcac agagcaaatc ttttttatga tgtgcttgta gattgcttga accgacttga
    98881 aaaagaaaca aatcgatttg aagtgacgat tacgtctgct catcctctaa ctgatgaaca
    98941 gaagactcgt ttgctccctt tgattgagaa aaaaatgtct ctgaaagtaa ggagtgtaaa
    99001 agaacaaatc gatgaaagtc tcattggtgg ttttgtcatt tttgccaatc acaagacaat
    99061 tgatgtgagt attaaacaac aacttaaagt tgttaaagaa aatttgaaat agaaagtggt
    99121 gttcttttgg caattaacgc acaagaaatc agcgctttaa ttaagcaaca aattgaaaat
    99181 ttcaaaccca attttgatgt gactgaaaca ggtgttgtaa cctatatcgg ggacggtatc
    99241 gcgcgtgctc atggccttga aaatgtcatg agtggagagt tgttgaattt tgaaaacggc
    99301 tcttatggta tggctcaaaa cttggagtca acagacgttg gtattatcat cctaggtgac
    99361 tttacagata tccgtgaagg cgatacaatc cgccgtacag ggaaaatcat ggaagtccct
    99421 gtaggtgaaa gtctgattgg tcgtgttgtg gatccgcttg gtcgtccagt tgacggtctt
    99481 ggagaaatcc acactgataa aactcgtcca gtagaagcac cagctcctgg tgttatgcaa
    99541 cgtaagtctg tttcagaacc attgcaaact ggtttgaaag ctattgacgc ccttgtaccg
    99601 attggtcgtg gtcaacgtga gttgattatc ggtgaccgtc agacagggaa aacaaccatt
    99661 gcgattgata caatcttgaa ccaaaaagat caagatatga tctgtatcta cgtcgcgatt
    99721 ggacaaaaag aatcaacagt tcgtacgcaa gtagaaacac ttcgtcagta cggtgccttg
    99781 gactacacaa tcgttgtgac agcctctgct tcacaaccat ctccattgct cttcctagct
    99841 ccttatactg gggttgctat ggcggaagaa tttatgtatc aaggtaagca tgttttgatt
    99901 gtatacgatg atctatcaaa acaagcggta gcttatcgtg aactatcgct cttgcttcgt
    99961 cgtcctccag gtcgtgaagc cttcccaggg gatgttttct atctccacag ccgtttgctt
   100021 gagcgctcag ctaaagtttc tgatgaactt ggtggtggat caattacagc cctaccattt
   100081 atcgagacac aagcaggaga tatctcagcc tatatcgcaa ccaacgtgat ttctatcact
   100141 gatggacaaa tcttccttgg cgatggcctc ttcaatgcag gtattcgtcc agccatcgat
   100201 gcgggttcat ctgtatctcg tgtaggtggt tctgcacaaa tcaaagccat gaagaaggtt
   100261 gctggtacac ttcgtatcga ccttgcttca taccgtgagt tggaagcctt tactaagttt
   100321 ggttctgact tggacgcagc aacacaggct aagttgaacc gtggacgtcg taccgttgag
   100381 gtcttgaaac aacctgttca caaaccatta cctgttgaga aacaagtaac cattctttat
   100441 gctttgacac atggtttctt ggatactgtt ccagtagatg atattgttcg tttcgaggaa
   100501 gagttccatg ccttctttga tgctcaacat ccagagattt tggaaaccat tcgtgataca
   100561 aaagacttgc cagaagaagc agtcttggat gctgcgatta cagagtttct caatcaatct
   100621 agcttccaat aagaatagag gtgtcagatg gcagtgtctc taaatgatat taaaacaaaa
   100681 atcgcctcaa caaaaaatac gagtcaaatc actaatgcca tgcaaatggt atcggctgct
   100741 aagttaggtc gctctgaaga agctgctcgc aacttccaag tttacgctca gaaagtgcgt
   100801 aaacttttga cagatatcct tcatggtaat ggagctggtg cttcaactaa tccgatgttg
   100861 attagccgtt ctgtgaagaa gacaggctat atcgttatca cttcagaccg cggtttggtt
   100921 ggaggttata attcctctat tttgaaagct gttatggagt tgaaagaaga ataccaccca
   100981 gacggtaaag gttttgaaat gatctgtatc ggtgggatgg gagctgattt ctttaaggct
   101041 cgcggtattc aaccacttta tgaattacgt ggcttggcag accaacctag ctttgatcaa
   101101 gttcgtaaga ttatttcaaa aactgttgaa atgtaccaaa atgaactctt tgatgagctt
   101161 tatgtttgct acaaccacca tgtcaatacg ctaaccagtc aaatgcgtgt ggaacaaatg
   101221 cttccgattg ttgacttgga tccaaatgaa gcggatgaag agtacagctt gacttttgaa
   101281 ttggaaacca gccgagaaga aattctggag cagttgttgc ctcagtttgc agaaagtatg
   101341 atttacggtg ccattatcga tgccaagaca gctgagaatg ctgcgggtat gacagccatg
   101401 caaacagcga cagataatgc taagaaagtc atcaatgatt tgacaattca gtataaccgt
   101461 gccagacagg cggcgattac acaagaaatt acagaaatcg tagcaggtgc tagtgcctta
   101521 gaataggctc tagtccagct cgtatgaaaa tgaacttagg acctagttga gctaggaacc
   101581 gacagtatct tatatagaat aggagaagga gatgagttca ggtaaaattg ctcaggttat
   101641 cggtcccgtt gtagacgttt tgtttgcagc aggggaaaaa cttcctgaga ttaacaatgc
   101701 acttgtcgtc tacaaaaatg acgaaagaaa aacaaaaatc gtccttgaag tagccttgga
   101761 gttaggagat ggtatggttc gtactatcgc catggaatca acagatgggt tgactcgtgg
   101821 aatggaagta ttggacacag gtcgtccaat ctctgtacca gtaggtaaag aaactttggg
   101881 acgtgtcttc aacgttttgg gagataccat tgatttggaa gctcctttta cagaagacgc
   101941 agagcgtcag ccaattcata aaaaagctcc aacttttgat gagttgtcta cctcttctga
   102001 aatccttgaa acagggatca aggttattga ccttcttgcc ccttacctta aaggtggtaa
   102061 agttggactt ttcggtggtg ccggagttgg taaaaccgtc ttaatccaag aattgattca
   102121 caacattgcc caagagcacg gtggtatttc agtatttact ggtgttgggg aacgtactcg
   102181 tgaggggaat gacctttact gggaaatgaa agaatcaggc gttatcgaga aaacagccat
   102241 ggtctttggt cagatgaatg agccaccagg agcacgtatg cgtgttgccc ttactggttt
   102301 gacaatcgct gaatacttcc gtgatgtgga aggccaagac gtgcttctct ttatcgataa
   102361 tatcttccgt ttcactcagg ctggttcaga agtatctgcc cttttgggtc gtatgccatc
   102421 agccgttggt taccaaccaa cacttgctac ggaaatgggt caattgcaag aacgtatcac
   102481 atcaaccaag aagggttctg taacctctat ccaggctatc tatgtgccag cggatgacta
   102541 tactgaccca gcgccagcaa cagccttcgc tcacttggat tcaacaacaa acttggaacg
   102601 taagttggta caattgggta tctacccagc cgttgaccca cttgcttcaa gctcacgtgc
   102661 cttggcacct gaaatcgttg gagaagagca ctatgcagtt gctgctgaag taaaacgtgt
   102721 ccttcaacgt taccatgaat tgcaagatat cattgctatc cttggtatgg atgagctttc
   102781 tgatgaagaa aagaccttgg ttgctcgcgc ccgtcgtatc cagttcttct tgtcacaaaa
   102841 cttcaacgtt gcggaacaat ttactggtca gccaggttct tatgttccag ttgctgaaac
   102901 tgtacgtggc tttaaggaaa tccttgatgg taaatacgac cacttgccag aagatgcctt
   102961 ccgtggtgta ggttctatcg aagatgtgat tgcaaaagct gaaaaaatgg gattttaaga
   103021 ggtgatctat ggctcagtta actgtccaga tcgtgacacc agatggtctc gtctatgatc
   103081 accatgccag ctatgtatcg gttcgaactc tggatggtga gatggggatc ttgccacgac
   103141 atgaaaatat gattgcggtt ttagcagttg atgaagtaaa ggtaaaacgt atcgatgata
   103201 aagatcacgt gaactggatt gcagtaaacg gtggcgttat tgaaattgcc aatgatatga
   103261 tcacaatcgt cgctgactct gcagaacgtg ctcgtgatat cgatatcagt cgtgcagaac
   103321 gtgccaaact tcgtgcagaa cgtgcaattg aagaagcaca agacaaacat ttgattgacc
   103381 aagaacgtcg tgctaagatt gctttgcaac gtgctattaa ccgtattaat gtcggaaata
   103441 gactataaga aaaaatgaac ttgaaaatac caagttcatt ttttatggtg ttttaaggag
   103501 caaaacggat gcagactgct tcgggaacat ggaagtcgtt ggagagttct gctagacgac
   103561 cattgtcaca attacgttta aagacagttg cattgtcaga gtcttgatgg acaacaatga
   103621 gaaatttttg gtcgggtgtc aaatcaaaat cacgtggagt ctgaccatgc gttggaacga
   103681 tttctaataa ctctaagcta ccgtccgcaa ggatggtata tactgcgata gaatcatggc
   103741 cacggttaga agcgtagagg tatttaccgt ctttagagag atgaatagca gcggttccat
   103801 taaagccttc gtaagcttct ggtaaagttg aaataacctg catacgttca aattcgccaa
   103861 cgccatcgta gattaaaact tcgatagtac tattgagttc acaaatgaga taagcgattt
   103921 tatagtggtt atggaaaatg atatggcgtg agcctgctcc tggcttgctg tgataggtat
   103981 agagcttaga taattttcct tcttgatcga ggtcataggt gatgacttgg tcagttccca
   104041 agtcgcaggt cactagatag tggtcaggtg ttaaatctgt atagtgaaca tggggggaag
   104101 cttgattttc atgtggacct tggccactgt gttgatccat atcactaagt agaagactac
   104161 catcttcctg gcgtttataa acaaggactt gtcccttgtg atagttggct gcgtaaacca
   104221 aatcacgctt ttcatcgaca gcaacataac agtggggagc tccttcttca acaacatgat
   104281 ttaacacagt cccgtcagtt tgataggctg caattccccc cttatcgtct tggctaccaa
   104341 cagtgtataa atgttggtgc tggtcaaagg caaggtaggt tggacttggc tcagctgcaa
   104401 aaagttctag atttgaaagc tgaccagttt ctgtatcaaa gtctgccttg taaatccctt
   104461 gagaagtacg acgtgtataa gttccaaaat aaacagtttc tttcattact atacctctgt
   104521 gtaaagataa gactattata tcacaaaaac aagcaaatta aagacattca attagatgta
   104581 agcactttaa aaaagagtta ttttgtttca aaaatggtat aatgagagaa caatagaaag
   104641 gaagtattta tggagcaaaa agagaaacat tttagcctat cttggttttt caagtggttt
   104701 ttagataaca aggcaattac ggtattttta gtaaccttat tattgggatt gaatcttttt
   104761 attttaagta agattagttt tctattttca cctgttttag actttatagc agttgtgatg
   104821 ttgccagtca ttttgtctgg tttgttatat tatttgttga atcctattgt tgattggatg
   104881 gagaagcata aggttaatcg tgttatagct atcactattg tctttgttat catcgctctc
   104941 tttatcattt ggggcttggc agtcgccatt ccaaatctgc aacgtcaggt tttgaccttt
   105001 gcaagaaacg ttcctgttta cttagaagat atagatagga ttgttaatgg attggtagcc
   105061 cagcacctgc cagatgattt cagacctcaa ttagagcaag ttttgaccaa tttttctagc
   105121 caggctacag ttttggcaag taaggtttca tctcaggcag tcaactgggt gagtgccttt
   105181 attagcggag cttctcaagt gattgttgcc ttgattatcg ttcctttcat gctcttttat
   105241 ctcttgcgtg atgggaaagg cttgcgtaac tatttgaccc aattcattcc aagaaaattg
   105301 aaggaacctg ttggacaagt tctatcagat gtgaatcaac agttgtccaa ctatgttcga
   105361 gggcaagtga cagtggctat tattgtagca gtaatgttta tcatcttctt caagattatt
   105421 ggtctacgct atgcggttac gctgggggtt actgctggta ttttaaatct ggtcccttat
   105481 cttggtagct ttctagccat gcttcctgcc ctagtattgg gtttgattgc tggtccagtc
   105541 atgcttttga aagtagtgat tgtctttatt gtagaacaaa ctattgaagg ccgttttgtc
   105601 tctccattga ttttgggaag tcaattaaac atccatccta ttaatgttct ctttgttttg
   105661 ttaacttcag gatctatgtt tggtatctgg ggagttttac ttggtattcc ggtttatgcc
   105721 tctgctaagg ttgtcatttc agccattttc gaatggtata aggtagtcag tggtctatat
   105781 gaattagagg gtgaggaagt caagagtgaa caatagtcaa cagatgttac aggctttgga
   105841 ggagcaagat ttaactaagg ctgagcatta tttcgccaaa gctttagaaa atgattcaag
   105901 tgatcttctg tatgagttgg caacttatct tgaagggatt ggtttctatc ctcaggccaa
   105961 ggaaatttac ctgaaaattg tagaggattt tccagaggtt catcttaatc tagctgcaat
   106021 tgctagcgag gatggtcaaa tagaagaagc ctttacctat cttgaggaaa tccaagctga
   106081 cagtgactgg tatgtctcgt ctttggctct gaaggcagac ctttaccagc tggaaggttt
   106141 gacagatgtg gcacgtgaga aattattgga ggccttgacc tactcagagg attctctctt
   106201 gatattgggt ttggcagagt tggatagtga gttggaaaat taccaagcgg ctattcaagc
   106261 ctatgcccag ttagataatc gctcgattta tgagcaaacg ggcatttcca cctatcaacg
   106321 aattggcttt gcctatgctc agttagggaa atttgaaacg gctactgagt ttttagaaaa
   106381 agccctggag ttagaatacg atgacttaac agcttttgag ttggccagtc tttattttga
   106441 tcaagaagaa tatcaaaaag ccaccctcta ctttaagcag cttgatacca tttctcctga
   106501 ctttgaaggc tatgagtatg ggtacagtca ggctttacat aaggaacatc aagttcaaga
   106561 agccctgcgt atcgctaagc aaggattaga gaaaaatccc tttgaaactc gcctcttgct
   106621 agctgcttca caattttctt atgaattgca tgatgctagt ggtgcagaaa attatctcct
   106681 tactgcaaaa gaagacgctg aggatacaga agaaatcttg cttcgtttag ccactattta
   106741 tctggagcag gagcgttatg aggatattct agacttgcag agtgaggagc cagaaaatct
   106801 tttgaccaag tggatgattg ctcgttctta tcaagaaatg gacgatttgg atactgctta
   106861 tgagcattat caagagttga caggagattt gaaggacaat ccagaatttc tggaacacta
   106921 tatctatctc ttgcgtgaat tgggacattt tgaagaagca aaagtccatg ctcacactta
   106981 cttaaaactg gttccagatg atgtgcaaat gcaagaactg tttgagagat tgtaagaatg
   107041 tttaaacata tagaactgta gtttatctct tttgatagct acggttttta tttgtatatg
   107101 gtagaatctt tttacaaaaa tacttggtaa tcttgtttat tcatgctata ataggaacaa
   107161 ttacttttag gaggtgcagt atgtcttatt tatttgagat attaccgagt ttactgaatg
   107221 gtgcgagcac gactgtacag gtctttgcac tggtcttgct attttcgatt cccttgggcg
   107281 ttttgattgc ctttgccttg caagtccatt ggaagcccct ccattatctg attaacattt
   107341 acatctgggt tatgcgagga acccccttac tcttgcaact gatttttatc tattatgtgc
   107401 tcccaagtat tgggattcgt ttagaccgcc ttcctgcagc tattattgcc tttgttttga
   107461 attatgcggc ttactttgca gaaattttcc gtgggggaat tgacactatt ccaagaggac
   107521 agtatgaggc tgccaaggtc ttgaagttta gcccttttga cagagtgcgc tatattatct
   107581 tgccccaagt gaccaagatc gttcttccta gtgtctttaa tgaagttatg agtttggtca
   107641 aggatacttc tttggtctat gctctcggaa tttcagacct tatcttggct agtcgaacag
   107701 ctgctaaccg cgatgctagt ctggttccta tgttcttggc aggagccatt tatttgattt
   107761 tgattgggat tgtgacaatt atttccaaaa aagttgagaa gaagtatagt tattatagat
   107821 aggaggctgc catgttagaa ttacgaaata tcaataaagt ctttggagac aaacaaatcc
   107881 tgtctaattt cagtctaagt attcctgaaa agcaaatcct ggctatcgtt ggaccttctg
   107941 gtggaggtaa gacaactctt ttacgtatgc ttgcaggtct tgaaaccatt gattcagggc
   108001 aaatctttta taatggacaa cctttagagc tggatgaatt gcagaagcgc aacctactgg
   108061 gatttgtctt ccaagatttt caactatttc ctcatctatc agttctggaa aatttgactt
   108121 tatcgcctgt gaagaccatg ggaatgaagc aggaagaggc tgagaagaag gcgagtggac
   108181 tcttggaaca gttaggacta ggaggacacg cagaggccta tcctttctca ctatctggtg
   108241 ggcaaaagca gcgagtggct ttggcgcgtg ctatgatgat tgacccagaa atcattggct
   108301 acgatgaacc aacttctgcc ctggatccag aattacgttt ggaagtggag aagctaatct
   108361 tgcaaaatag ggaacttggg atgacccaga ttgtggttac ccatgatttg cagtttgctg
   108421 aaaatatcgc agatgtatta ttgaaagtag aacctaaata ggaggaaaaa tggatgaaaa
   108481 aatggatgct tgtattagtc agtctgatga ctgctttgtt cttagtagct tgtgggaaaa
   108541 attctagcga aactagtgga gataattggt caaagtacca gtctaacaag tctattacta
   108601 ttggatttga tagtactttt gttccaatgg gatttgctca gaaagatggt tcttatgcag
   108661 gatttgatat tgatttagct acagctgttt ttgaaaaata cggaatcacg gtaaattggc
   108721 aaccgattga ttgggatttg aaagaagctg aattgacaaa aggaacgatt gatctgattt
   108781 ggaatggcta ttccgctaca gacgaacgcc gtgaaaaggt ggctttcagt aactcatata
   108841 tgaagaatga gcaggtattg gttacgaaga aatcatctgg tatcacgact gcaaaggata
   108901 tgactggaaa gacattagga gctcaagctg gttcatctgg ttatgcggac tttgaagcaa
   108961 atccagaact tttgaagaat attgtcgcta ataaggaagc gaatcaatac caaaccttta
   109021 atgaagcctt gattgatttg aaaaacgatc gaattgatgg tctattgatt gaccgtgtct
   109081 atgcaaacta ttatttagaa gcagaaggtg ttttaaacga ttataatgtc tttacagttg
   109141 gactagaaac agaagctttt gcggttggag cccgtaagga agatacaacc ttggttaaga
   109201 agataaatga agctttttct agtctttaca aggacggcaa gttccaagaa atcagccaaa
   109261 aatggtttgg agaagatgta gcaaccaaag aagtaaaaga aggacagtaa gataaaatag
   109321 tggctgagac tacgttttga ttagcaaaac gtagtttttt ttgtaatcta ggaaaacgat
   109381 aatagcgatt gaatctggat aattgaatat ggaatagccc actgtgattt ctaaaacatt
   109441 gttaaaaatt gatttgactt ccaaaattaa aatgttctgt aatgaaatac tgatgtaact
   109501 gttttaggaa caataaaacg cataatatca aggtttttgc accttacatt atgcgttttt
   109561 gtgattttaa gacttgttag ctgatttttt acaatcctac gaaatctttg atttcttgtg
   109621 ctgacattga agagtcgcaa cggacgttga tttgtccatc tgtaatatga acaaaacctg
   109681 gtacagttgg gattccataa cgtgagcgga atgcttgcaa atcattgagt tggcttggtt
   109741 cttcactatt gatgaagtaa atgtgagctt tggtttcagc tacgacacct gacaatgtac
   109801 ctgcaaattt acggcagtaa gggcaagttt tgcgaccgat aaagaaggtt gcagtttctt
   109861 ttttatcaag agcttcttgc gcacgcacaa ctgtagtgac ttcaaggtct ttgatgttat
   109921 ctaaaaattg ttccatgaga ttacctcgct ttcattgata agtctagtat gccataaagt
   109981 ttctaaaatt gcttagattt gatacgaaaa aagatgaggt tggttggtct catcttttat
   110041 aggtctttat tttacaaatg cattgatttc tgcttcgatg ttagtaatct tagcttgtga
   110101 ttcttcgttg gtttccccta caactgcaat gtagaacttg atttttggtt ctgtacctga
   110161 agggcgaacg gcaatccatg aaccgtcagc aagtgtgtat ttcaacacat cacttggagg
   110221 agttgtcaag tttgtaacag taccgtcagc aacagtagca gtttgtgcct tgaagtcttc
   110281 tacgacagtg atagctgttg cgttccattc tgttggagca ttgttgcgga atttagccat
   110341 aatcgctttg atttgttcag caccatcgac acctgaaaga gtaacagaga ttgttttttc
   110401 tgcgtagtag ccatattctt tatagatttc ttcgataccg tcagcaagtg tcaaaccacg
   110461 agaacggtag taggcagcaa gttcagcaac tacaagaacg gcttggatgg catctttatc
   110521 acgtacaaat ggtttaatca agtaaccgaa gctttcttca aatcccatca tgtaagtgtg
   110581 gttgtgtttt tcttcgaatt cttggatttt ttcagcgata aatttgaaac ctgtcaagac
   110641 gttgaacata gttgcgccgt agctttcagc aatcttcgtt accaagtcag ttgaaacgat
   110701 agatttgcag agagcggcat tttcaggaag agttccagcg tttttgtgag cttccaagat
   110761 gtatttagcc atgatagcac cgatttggtt acctgaaagg ttgaggtagc tgccatcttt
   110821 ttgaagaact tcaacaccaa cacggtcagc gtctgggtca gttgcgacaa gaacatctgc
   110881 accaacttga cgaccaagtt cttcagcaag ggcaaaggct gcttggcttt ctgggtttgg
   110941 agatgttaca gttgagaagt ctgggtcagc agttgcttgc gcttcaacaa cttgaacaga
   111001 gtcaaatcct gcttgggcaa gagcacgacg agccaacatt tcaccagtac catgaagtgg
   111061 tgtgtagaca atcttcatgt ctttaccaaa ttcttcaatc aaggctgggt tgatgtttat
   111121 gtccttaacc tctttaaggt attctatgtc aacagcttcg ccgataactt caatcaagcc
   111181 agaagctttt tcagtttcca catcagcaac ttcaactgca aatgggtttt cgattgcacg
   111241 gatataagta gtcaaagcgt ccgcatcgtg tggaggcatt tgtccaccgt cttcaccgta
   111301 aaccttgtaa ccgttaaatg gagcagggtt gtggctggct gtgaccatga tacctgcgaa
   111361 acagttgaga tgacgaactg caaatgatag ttctggagtt ggacgaaggc tttcaaatac
   111421 gtaagatttg atgccgtgtt tagcaagaac tgccgcagat tcaaaggcaa actcaggtga
   111481 gaagtgacgg ctatcgtagg caattgctac accgcgttct ttctcgtttc caccttttga
   111541 ctcaatcaaa cgagccaatc cttcagtagc ttggcgaaca acgtagatgt tgatacggtt
   111601 tgtaccagca ccaaccaagc cacgcatacc tgcagtacca aattcaagat ttgtatagaa
   111661 ggcatcttcc ttagtttttt cgtccatatt ttccaaatct tgacgaaggt agtcaggaag
   111721 ctccacaaaa tcaacccatt tctggtaatt ttcttggtaa gacattcaaa ttctccttta
   111781 tttttaaaac atttaatcag tttgattata tcattttttt tagttttagt aaaaccttat
   111841 ctgcttcgaa aatctcttca aaccaggtca gattgaattt tggggttata tgatgtttag
   111901 gctaggaaaa attcaatttc agtaaaaaaa gtaagtcttc tcataacaaa acattgatat
   111961 agttacttag ttttaaacaa gcatattata ataaagctat ggcatatagt actgatttta
   112021 aacagcgagc attagattac atcaaagagg ggcacagcca tgtcgaggca gccaagtttt
   112081 ttggtgttgg cgtcagaact ctcttcacgt gggaaaagaa agacgtgaac aagaacacat
   112141 agagaggaaa aagcgagtcg tcaaaaaccg aaagattcct ttagaggaat tgaaagcctt
   112201 tgtagaggct catccagatg cttttttacg ggaaattgcg gcacattttg attgtgctgt
   112261 tccttcagta tgggcagctt taaagcagat taaggtcact ttaaaaaaag atgacgagct
   112321 ttaaggaaca agacccagaa aagtagcctt atttcttaag aattttaata gtttaaagca
   112381 cctagcacct gtttatattg atgaaacagg aatcgaccgc tatctctatc gtccttatgc
   112441 aggggctcct agaggggaga aagtctatga aaagattagc ggacgtcgtt ttgagcgaat
   112501 ttcaattgtt gcaggacaag tagacggaga gtttatagct cccatgattt acaagaaaag
   112561 catgacaagc gatttctttg tggagtggtt caaaacgcaa ctcctacctg ctttgaagac
   112621 acctcatgtt attgtcatgg gcaatgctgg ttttcatccc aagaacattt tggatgaact
   112681 ctgcatccaa gataaacact ttttcttacc tctaccacct tattcaccgg atttgaatcc
   112741 tattgagcaa gcttgggcta tcttgaaaaa gaaagtgacg gatttattaa gggaagttcc
   112801 aactattttt gaatgtttgg aatgcttttt taaaactaga tgactataac ggttctaaag
   112861 gaacctatcg agtagtcatt aaaactaagg atactgctgg ttaagagaag acggtataca
   112921 atcaaaccat tcaccgtgta gccgaaatcg ttcagaatga agacttgtat aaaaaaggtt
   112981 tgaatgttga acttgcgcac caacaaatta agggattttt tgaagcagag tttaaaaatc
   113041 gtattaatgg agttcttaat actaaaataa aaaatagtac attaaatcgt gtaaataaaa
   113101 aaactataca ccagagcaac aaaaactcca tgatcaattt gaagcagaag caacggaaga
   113161 tgctaaaaaa caaggcgata ttgtgttgaa tgttgaccag gatttcatga gcatatctaa
   113221 gtctaataaa agtggttcag actggaagaa aactttcaca gtgaggacaa ccaataggct
   113281 agcaaatgac ttgaataatg tcttgaaaca ggctgataaa gatactccta ataccccaac
   113341 ttggctaaac tcagctgctt ctaaagctaa agatgatgac agagcatata aactactgaa
   113401 gactcttata ccaggagaaa attacctatc atgttaagga taatcagcta gaagtagaaa
   113461 cagataaata cacatatact gccgctagaa atggtagtaa ggaagttggt attcaagagt
   113521 cagatatagc agcaactcta agtgccgatg aatataattc taatcgccaa acttttgaga
   113581 gagaatacaa atacaaaagc aaatgccctt aataatggtt gggctagatc tggttctgaa
   113641 gagttcaaaa agttctccca ctttgtaggg gtagacaaag ggattgtgcg aacgaatgta
   113701 ctgactggta aaaaactatc tgataagatt aggaaagaag tgggctctgg agatagcaaa
   113761 ctaggaaaag gcggctattt ctctactggg gatgttctat taggaaaaga tgttgtttct
   113821 tataccgtac aagtattttc agagaataat gaaagagtag gagtaaacac tcaaagtcac
   113881 cgtgttcagt ataatctccc aattctagct gacttttcag tcatccaaga tactgtggaa
   113941 ccatcacgaa ccgttgttga aaaaatcatt ccaaaactaa atattcccga agaagagaaa
   114001 gggaaaataa ccgaagaaat caagaaaaag aaaaaaacct cagaattggc agaactaatc
   114061 tcagaaaatg tgaaagttcg ctatgttgat gaacaagggc gtttgctatc attgaaaaat
   114121 gatactggaa ttggagaaaa agaaagtgac ggaacctaca ttaccaataa aaaacaactg
   114181 attggtacca gctacaatgt cacagataaa aaactcagta gcatgactac tactgacgga
   114241 aaatattata cttttaaaga agcagataca aattctgcaa gtttaactgg gaatattgta
   114301 agcgaaggta gaacagtgac cttagtttat agagaaagcg aagcgccaac cactgctaca
   114361 gtaacagcca attactataa agaaggtagg caagagaagt tggtagagtc tgttataaaa
   114421 gctgatttag cgataggttc tgagtatacc acagaatcaa aaactattga agggaaaaca
   114481 acaactgagg acaaagaaga ccgagttatc acaaggaaaa caacatacac cttggtagca
   114541 actcctgcaa atgcgtacca gaagacggtg caacagttga ctattactac cgtgagaatg
   114601 ttgaggaaac agtggttccc aaaacagcaa cctctactga gacgaagact ataacgcgta
   114661 tcattcatta cgttgataaa gttacgaacc aaaatgtaaa agaagatgtt gttcaacctg
   114721 taaccttaag ccgtacaaaa actgagaaca aggtcacggg agttgtaacc tacggtgaat
   114781 ggacaacagg aaactgggac gaggttatat ctggtaagat tgacaagtac aaagatccag
   114841 atattccaac agttgaatca caagaagtta cgtcagactc tagtgataaa gaaataacgg
   114901 taaggtatga ccgtttatca acaccagaaa aaccaatccc acaaccaaat ccagagcatc
   114961 caagtgttcc gacaccaaac ccagaactac caaatcaaga gactccaaca ccagataaac
   115021 caactccaga accaggtact ccaaaaactg aaactccagt gaatccagac ccagaagttc
   115081 cgacttatga gacaggtaag agagaggaat tgccaaacac aggtacagaa gctaatgcta
   115141 ccttggctag tgctggtatc atgaccttgt tagctggtct aggattagga tttttcaaga
   115201 aaaaagaaga tgaaaaataa tagattttag aatctaggaa ccaggaaaag ctcacagatg
   115261 tgggcttttt tcctggtttt gagaacgagg tctttcgtaa agaataaaaa cgcttacaag
   115321 tctgttgaac tgggaaacta tgaatcctat ttttttaaaa atatttccag aaatcagttg
   115381 ctaaataggg taaaaattga taaaataagg aatatctaaa aaattttaag gagaatctag
   115441 caaatggatt tcacatgggc actgaagtat gccactgaat ttttgggaac tgccattttg
   115501 atcattcttg ggaatggtgc agttgccaac gttgaactta aaggtacgaa aggtcaccaa
   115561 agtggctgga tcgtcatcgc tgttggttat ggtatggggg ttatgatccc agccttgatg
   115621 tttggtaacg tatctgggaa tcacatcaac cctgctttca ctctagggct tgcagttagc
   115681 ggtcttttcc cttgggcaca agtggtacct tacattatcg cgcaagtctt gggggctatc
   115741 tttggccaag ccttagttgt ggcaacatac cgtccattct acttgaaaac tgaaaaccca
   115801 aataacatct tgggaacttt ctcaactatt tcaagtattg accatggtac aaaagaaagt
   115861 cgctatgcag caactgtcaa tggtttgatt aatgagtttg ttggttcatt tgttttgttc
   115921 tttgcagctc ttggtttgac taaaaacttc tttggtgctg aagtgcttca attcatgaaa
   115981 caaaaggcaa tagaagcagg acaaacagtt gatttttctg acttggctat taaagcacag
   116041 gtggctccac acactgcttc aggactttct gtggctcact tggcacttgg attcctcgtt
   116101 atggctttgg taacatcact tggaggacct acaggacctg ccttgaaccc agcccgtgac
   116161 ttgggaccac gtctccttca tgctttcctt ccaaaatcag ttcttggtga gcataaaggc
   116221 gattcaaaat ggtggtattc ttgggtacca gtagtagcac ctatcgcagc agcaattgcg
   116281 gcagtagctg tattcaaatt cctttatctc taagaaatag ctcctttaac atttgagtga
   116341 gcaccatcta taagtaagag aggatcagac tggttctctc tttttgattt tttagggaaa
   116401 tgaaagaaac tctaaacaaa ctcctctcca gcagtggttt agaagtctca gtgggctatt
   116461 ccagcttcaa tgggctatag taggttgcag ttgaaataat agacccttgt ttctaaaaca
   116521 ttgttagaaa ttggtttgaa ttctccaatc aaattgtgca gttttcattc tactatatat
   116581 tatcggagat gggttcccta tcttgtaagt ctgctttata gtgggttgaa gttggaatag
   116641 tcctcccttc tttctcaaac attgtgagga attgatttac cttcctcaac aaaatgttca
   116701 gtttctattt cattttgcta taaaataagc gattaggggg gctattcttc gacctacatt
   116761 gactctgctg agtcctatga ttgttatcgt tttatctgca attttatact caatgaaaat
   116821 caaagggcaa actaagaagc tagccgcagg ttgttcaaaa cacagttttg aggttgtata
   116881 gtagattgaa actagaatag tacaccttaa cttctaaaac attgttagaa atcgatttga
   116941 ctgtcctgat cgatttgccc tattcttgtt tcattttact atataaacca gagactgttt
   117001 acattttcag caagtgagtg gatggataat gctgaaaact ccttgaagga taagtctatt
   117061 tagtactttc tattagttag ttaaattttt accaagaata attcacaaaa acgttgtaaa
   117121 acacttgcaa tttagctgaa atttgataaa atagtaagga aagttagact gtattgccta
   117181 ctgtctatct ataaaatata ttttattgga ggcttttact caaatggcaa aagaaaaata
   117241 cgatcgtagt aaaccacacg ttaacattgg tactatcgga cacgttgacc acggtaaaac
   117301 taccctaact gcagctatca caactgtttt ggcacgtcgc ttgccttcat cagttaacca
   117361 acctaaagac tatgcgtcta tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat
   117421 caacactgcg cacgttgagt acgaaactga aaaacgtcac tacgctcaca tcgacgctcc
   117481 aggacacgcg gactacgtta aaaacatgat cactggtgct gctcaaatgg acggagctat
   117541 ccttgtagta gcttcaactg acggaccaat gccacaaact cgtgagcaca tccttctttc
   117601 acgtcaggtt ggtgttaaac accttatcgt cttcatgaac aaagttgact tggttgacga
   117661 cgaagaattg cttgaattgg ttgaaatgga aatccgtgac ctattgtcag aatacgactt
   117721 cccaggtgac gatcttccag ttatccaagg ttcagcactt aaagctcttg aaggtgactc
   117781 taaatacgaa gacatcgtta tggaattgat gaacacagtt gatgagtata tcccagaacc
   117841 agaacgtgac actgacaaac cattgcttct tccagtcgag gacgtattct caatcactgg
   117901 acgtggtaca gttgcttcag gacgtatcga ccgtggtatc gttaaagtca acgacgaaat
   117961 cgaaatcgtt ggtatcaaag aagaaactca aaaagcagtt gttactggtg ttgaaatgtt
   118021 ccgtaaacaa cttgacgaag gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt
   118081 tcaacgtgat gaaatcgaac gtggacaagt tatcgctaaa ccaggttcaa tcaacccaca
   118141 cactaaattc aaaggtgaag tctacatcct tactaaagaa gaaggtggac gtcacactcc
   118201 attcttcaac aactaccgtc cacaattcta cttccgtact actgacgtta caggttcaat
   118261 cgaacttcca gcaggtactg aaatggtaat gcctggtgat aacgtgacaa tcgatgttga
   118321 gttgattcac ccaatcgccg tagaacaagg tactacattc tctatccgtg agggtggacg
   118381 tactgttggt tcaggtatgg ttacagaaat cgaagcttaa ttcgatttag ttcccagaag
   118441 aacaattatt taagtcagac actaaaagaa tcttgcttgg caaggttctt tttttagata
   118501 ttgaactaat actaaatgaa aatcaaagag caaactataa tatattgaaa ctagaatagt
   118561 acacatctac ttctaaaaca ttcttagaaa tcgatttgat tgtcttgatt gatttgtctt
   118621 gttcttattt cattttgtta tagaaagtta gctgcagact gctcaaaaca ctgtttttag
   118681 gttgtagata gaactgacga agtcagtaac atctatacga caaggcgaag ctgacgcggt
   118741 ttgaagagat tttcgaagag tataatacta gactcaaat
//
LOCUS       DAVKMU010000002        98028 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKMU010000002 DAVKMU010000000
VERSION     DAVKMU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 98028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.002"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..588
                     /locus_tag="VW343_000141"
     CDS             <1..588
                     /locus_tag="VW343_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754770.1"
                     /translation="SLIKLDQMGLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSH
                     GANLSLYEYCLLDNSLTLKNDWNYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVP
                     FVFLDLIGEGKKEYEMFFQWLNFFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIF
                     KICPGVYKGLSYYLMKFLLKSFSNEYLKTTDEVNR"
     gene            585..1763
                     /locus_tag="VW343_000142"
     CDS             585..1763
                     /locus_tag="VW343_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9754771.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            2188..2421
                     /locus_tag="VW343_000143"
     CDS             2188..2421
                     /locus_tag="VW343_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9754772.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            2704..4653
                     /gene="mutL"
                     /locus_tag="VW343_000144"
     CDS             2704..4653
                     /gene="mutL"
                     /locus_tag="VW343_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9754773.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(4978..5445)
                     /locus_tag="VW343_000145"
     CDS             complement(4978..5445)
                     /locus_tag="VW343_000145"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9754774.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIAVAWIPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(5446..6651)
                     /locus_tag="VW343_000146"
     CDS             complement(5446..6651)
                     /locus_tag="VW343_000146"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9754775.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(6671..7306)
                     /gene="ribE"
                     /locus_tag="VW343_000147"
     CDS             complement(6671..7306)
                     /gene="ribE"
                     /locus_tag="VW343_000147"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9754776.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(7291..8391)
                     /gene="ribD"
                     /locus_tag="VW343_000148"
     CDS             complement(7291..8391)
                     /gene="ribD"
                     /locus_tag="VW343_000148"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9754777.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(8526..8640)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8797..9390
                     /gene="ruvA"
                     /locus_tag="VW343_000149"
     CDS             8797..9390
                     /gene="ruvA"
                     /locus_tag="VW343_000149"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9754778.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            9400..9963
                     /locus_tag="VW343_000150"
     CDS             9400..9963
                     /locus_tag="VW343_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9754779.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            9960..10637
                     /locus_tag="VW343_000151"
     CDS             9960..10637
                     /locus_tag="VW343_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9754780.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIATYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVDYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(10792..11823)
                     /locus_tag="VW343_000152"
     CDS             complement(10792..11823)
                     /locus_tag="VW343_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9754781.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKVLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEEPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDNNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(12078..12761)
                     /locus_tag="VW343_000153"
     CDS             complement(12078..12761)
                     /locus_tag="VW343_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9754782.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(12773..13717)
                     /locus_tag="VW343_000154"
     CDS             complement(12773..13717)
                     /locus_tag="VW343_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9754783.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            13849..16680
                     /gene="uvrA"
                     /locus_tag="VW343_000155"
     CDS             13849..16680
                     /gene="uvrA"
                     /locus_tag="VW343_000155"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9754784.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            16673..17734
                     /locus_tag="VW343_000156"
     CDS             16673..17734
                     /locus_tag="VW343_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9754785.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLFPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            17866..18264
                     /gene="spx"
                     /locus_tag="VW343_000157"
     CDS             17866..18264
                     /gene="spx"
                     /locus_tag="VW343_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9754786.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            18330..18899
                     /locus_tag="VW343_000158"
     CDS             18330..18899
                     /locus_tag="VW343_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9754787.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            18986..19252
                     /locus_tag="VW343_000159"
     CDS             18986..19252
                     /locus_tag="VW343_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9754788.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            19256..19675
                     /gene="ruvX"
                     /locus_tag="VW343_000160"
     CDS             19256..19675
                     /gene="ruvX"
                     /locus_tag="VW343_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9754789.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            19691..19996
                     /locus_tag="VW343_000161"
     CDS             19691..19996
                     /locus_tag="VW343_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9754790.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            20409..21659
                     /locus_tag="VW343_000162"
     CDS             20409..21659
                     /locus_tag="VW343_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9754791.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            21656..22213
                     /locus_tag="VW343_000163"
     CDS             21656..22213
                     /locus_tag="VW343_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9754792.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            22359..23891
                     /gene="cls"
                     /locus_tag="VW343_000164"
     CDS             22359..23891
                     /gene="cls"
                     /locus_tag="VW343_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9754793.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGINLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            23969..24112
                     /locus_tag="VW343_000165"
     CDS             23969..24112
                     /locus_tag="VW343_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754794.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            24149..25687
                     /locus_tag="VW343_000166"
     CDS             24149..25687
                     /locus_tag="VW343_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9754795.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFEFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            25807..28014
                     /gene="nrdD"
                     /locus_tag="VW343_000167"
     CDS             25807..28014
                     /gene="nrdD"
                     /locus_tag="VW343_000167"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9754796.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            28026..28166
                     /locus_tag="VW343_000168"
     CDS             28026..28166
                     /locus_tag="VW343_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754797.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            28210..28716
                     /locus_tag="VW343_000169"
     CDS             28210..28716
                     /locus_tag="VW343_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9754798.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNLASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            28709..29299
                     /gene="nrdG"
                     /locus_tag="VW343_000170"
     CDS             28709..29299
                     /gene="nrdG"
                     /locus_tag="VW343_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9754799.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            29296..29913
                     /locus_tag="VW343_000171"
     CDS             29296..29913
                     /locus_tag="VW343_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9754800.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQASMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            30179..30487
                     /gene="rpsJ"
                     /locus_tag="VW343_000172"
     CDS             30179..30487
                     /gene="rpsJ"
                     /locus_tag="VW343_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9754801.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            30704..31330
                     /gene="rplC"
                     /locus_tag="VW343_000173"
     CDS             30704..31330
                     /gene="rplC"
                     /locus_tag="VW343_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9754802.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            31355..31978
                     /gene="rplD"
                     /locus_tag="VW343_000174"
     CDS             31355..31978
                     /gene="rplD"
                     /locus_tag="VW343_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9754803.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            31978..32274
                     /locus_tag="VW343_000175"
     CDS             31978..32274
                     /locus_tag="VW343_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9754804.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            32292..33125
                     /gene="rplB"
                     /locus_tag="VW343_000176"
     CDS             32292..33125
                     /gene="rplB"
                     /locus_tag="VW343_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9754805.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            33229..33510
                     /gene="rpsS"
                     /locus_tag="VW343_000177"
     CDS             33229..33510
                     /gene="rpsS"
                     /locus_tag="VW343_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9754806.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            33522..33866
                     /gene="rplV"
                     /locus_tag="VW343_000178"
     CDS             33522..33866
                     /gene="rplV"
                     /locus_tag="VW343_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9754807.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            33879..34532
                     /gene="rpsC"
                     /locus_tag="VW343_000179"
     CDS             33879..34532
                     /gene="rpsC"
                     /locus_tag="VW343_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9754808.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            34536..34949
                     /gene="rplP"
                     /locus_tag="VW343_000180"
     CDS             34536..34949
                     /gene="rplP"
                     /locus_tag="VW343_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9754809.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            34959..35165
                     /locus_tag="VW343_000181"
     CDS             34959..35165
                     /locus_tag="VW343_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9754810.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            35190..35450
                     /gene="rpsQ"
                     /locus_tag="VW343_000182"
     CDS             35190..35450
                     /gene="rpsQ"
                     /locus_tag="VW343_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9754811.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            35476..35844
                     /gene="rplN"
                     /locus_tag="VW343_000183"
     CDS             35476..35844
                     /gene="rplN"
                     /locus_tag="VW343_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9754812.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            35922..36227
                     /gene="rplX"
                     /locus_tag="VW343_000184"
     CDS             35922..36227
                     /gene="rplX"
                     /locus_tag="VW343_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9754813.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            36251..36793
                     /gene="rplE"
                     /locus_tag="VW343_000185"
     CDS             36251..36793
                     /gene="rplE"
                     /locus_tag="VW343_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9754814.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            36811..37080
                     /gene="rpsN"
                     /locus_tag="VW343_000186"
     CDS             36811..37080
                     /gene="rpsN"
                     /locus_tag="VW343_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9754815.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            37365..37763
                     /gene="rpsH"
                     /locus_tag="VW343_000187"
     CDS             37365..37763
                     /gene="rpsH"
                     /locus_tag="VW343_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9754816.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            37955..38491
                     /gene="rplF"
                     /locus_tag="VW343_000188"
     CDS             37955..38491
                     /gene="rplF"
                     /locus_tag="VW343_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9754817.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            38575..38931
                     /gene="rplR"
                     /locus_tag="VW343_000189"
     CDS             38575..38931
                     /gene="rplR"
                     /locus_tag="VW343_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9754818.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            38949..39443
                     /gene="rpsE"
                     /locus_tag="VW343_000190"
     CDS             38949..39443
                     /gene="rpsE"
                     /locus_tag="VW343_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9754819.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            39415..39639
                     /gene="rpmD"
                     /locus_tag="VW343_000191"
     CDS             39415..39639
                     /gene="rpmD"
                     /locus_tag="VW343_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9754820.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            39784..40224
                     /gene="rplO"
                     /locus_tag="VW343_000192"
     CDS             39784..40224
                     /gene="rplO"
                     /locus_tag="VW343_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9754821.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            40237..41547
                     /gene="secY"
                     /locus_tag="VW343_000193"
     CDS             40237..41547
                     /gene="secY"
                     /locus_tag="VW343_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9754822.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            41698..42336
                     /locus_tag="VW343_000194"
     CDS             41698..42336
                     /locus_tag="VW343_000194"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9754823.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            42453..42671
                     /gene="infA"
                     /locus_tag="VW343_000195"
     CDS             42453..42671
                     /gene="infA"
                     /locus_tag="VW343_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9754824.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            42696..42812
                     /gene="rpmJ"
                     /locus_tag="VW343_000196"
     CDS             42696..42812
                     /gene="rpmJ"
                     /locus_tag="VW343_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9754825.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            42830..43195
                     /gene="rpsM"
                     /locus_tag="VW343_000197"
     CDS             42830..43195
                     /gene="rpsM"
                     /locus_tag="VW343_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9754826.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            43213..43596
                     /gene="rpsK"
                     /locus_tag="VW343_000198"
     CDS             43213..43596
                     /gene="rpsK"
                     /locus_tag="VW343_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9754827.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43639..44574
                     /locus_tag="VW343_000199"
     CDS             43639..44574
                     /locus_tag="VW343_000199"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9754828.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            44586..44972
                     /gene="rplQ"
                     /locus_tag="VW343_000200"
     CDS             44586..44972
                     /gene="rplQ"
                     /locus_tag="VW343_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9754829.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            45237..45503
                     /locus_tag="VW343_000201"
     CDS             45237..45503
                     /locus_tag="VW343_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9754830.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            45513..46850
                     /locus_tag="VW343_000202"
     CDS             45513..46850
                     /locus_tag="VW343_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9754831.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            47094..47786
                     /locus_tag="VW343_000203"
     CDS             47094..47786
                     /locus_tag="VW343_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9754832.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(47889..49534)
                     /locus_tag="VW343_000204"
                     /pseudo
     CDS             complement(47889..49534)
                     /locus_tag="VW343_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(49524..50615)
                     /locus_tag="VW343_000205"
                     /pseudo
     CDS             complement(49524..50615)
                     /locus_tag="VW343_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(50628..51749)
                     /locus_tag="VW343_000206"
                     /pseudo
     CDS             complement(50628..51749)
                     /locus_tag="VW343_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(51966..52163)
                     /locus_tag="VW343_000207"
     CDS             complement(51966..52163)
                     /locus_tag="VW343_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9754833.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(52238..53014)
                     /locus_tag="VW343_000208"
     CDS             complement(52238..53014)
                     /locus_tag="VW343_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9754834.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            53136..53882
                     /locus_tag="VW343_000209"
     CDS             53136..53882
                     /locus_tag="VW343_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9754835.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            53895..54875
                     /locus_tag="VW343_000210"
     CDS             53895..54875
                     /locus_tag="VW343_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9754836.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            55070..55390
                     /locus_tag="VW343_000211"
     CDS             55070..55390
                     /locus_tag="VW343_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9754837.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            55436..55744
                     /locus_tag="VW343_000212"
     CDS             55436..55744
                     /locus_tag="VW343_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9754838.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            55761..57059
                     /locus_tag="VW343_000213"
     CDS             55761..57059
                     /locus_tag="VW343_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9754839.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLAMSTMVYFPFFKIQDRLACQNEIKQS"
     gene            57210..59648
                     /locus_tag="VW343_000214"
     CDS             57210..59648
                     /locus_tag="VW343_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9754840.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            59667..60335
                     /locus_tag="VW343_000215"
     CDS             59667..60335
                     /locus_tag="VW343_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9754841.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            60353..61441
                     /locus_tag="VW343_000216"
     CDS             60353..61441
                     /locus_tag="VW343_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9754842.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            61923..64424
                     /locus_tag="VW343_000217"
     CDS             61923..64424
                     /locus_tag="VW343_000217"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9754843.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            64618..65313
                     /locus_tag="VW343_000218"
     CDS             64618..65313
                     /locus_tag="VW343_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9754844.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            65325..65741
                     /locus_tag="VW343_000219"
     CDS             65325..65741
                     /locus_tag="VW343_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9754845.1"
                     /translation="MITIKKQEIVKLEDVLHLYQVVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(66556..66648)
                     /locus_tag="VW343_000220"
     CDS             complement(66556..66648)
                     /locus_tag="VW343_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9754846.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            66809..67780
                     /gene="ruvB"
                     /locus_tag="VW343_000221"
     CDS             66809..67780
                     /gene="ruvB"
                     /locus_tag="VW343_000221"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9754847.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            67761..68333
                     /locus_tag="VW343_000222"
     CDS             67761..68333
                     /locus_tag="VW343_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9754848.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            68564..69322
                     /locus_tag="VW343_000223"
     CDS             68564..69322
                     /locus_tag="VW343_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9754849.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            69331..70134
                     /locus_tag="VW343_000224"
     CDS             69331..70134
                     /locus_tag="VW343_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9754850.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            70156..71415
                     /gene="rseP"
                     /locus_tag="VW343_000225"
     CDS             70156..71415
                     /gene="rseP"
                     /locus_tag="VW343_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9754851.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            71428..73281
                     /locus_tag="VW343_000226"
     CDS             71428..73281
                     /locus_tag="VW343_000226"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9754852.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            73380..74759
                     /gene="bglA"
                     /locus_tag="VW343_000227"
     CDS             73380..74759
                     /gene="bglA"
                     /locus_tag="VW343_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9754853.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEHYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            74952..76760
                     /gene="glmS"
                     /locus_tag="VW343_000228"
     CDS             74952..76760
                     /gene="glmS"
                     /locus_tag="VW343_000228"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9754854.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGFDVDKPRNLAKSVTVE"
     gene            complement(76898..76975)
                     /locus_tag="VW343_000229"
                     /pseudo
     CDS             complement(76898..76975)
                     /locus_tag="VW343_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            77032..78081
                     /locus_tag="VW343_000230"
     CDS             77032..78081
                     /locus_tag="VW343_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9754855.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLALLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(78181..82017)
                     /gene="spuA"
                     /locus_tag="VW343_000231"
     CDS             complement(78181..82017)
                     /gene="spuA"
                     /locus_tag="VW343_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9754856.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNKSDISSLGNANASLEKTEEKPATEPT
                     TPAASPADPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQ
                     GLWTWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNL
                     TGDKSVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVK
                     NPSSAQWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFT
                     DLKNHSQIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKH
                     SNITNHLGNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDE
                     TYSYDGKLGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTW
                     KQTLDSTNKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSNDAKIDDAHKVA
                     KAAFVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEA
                     FIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSL
                     TGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFM
                     DADGTPRTSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEA
                     YKAARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGY
                     PNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSI
                     KKDPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDK
                     VPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKG
                     LIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVN
                     ADEKAREFNLGTAFAHLRNAEVLADENQAGPVGIANPKGLEWTEKGLKLNALTATVLR
                     VSQNGTSHESTAEEKPDSTPSKPEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQ
                     AEQPAQEAQASSVKEAVQNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGISL
                     LALLGLGFLLKNKKEN"
     gene            82434..82847
                     /gene="rpsL"
                     /locus_tag="VW343_000232"
     CDS             82434..82847
                     /gene="rpsL"
                     /locus_tag="VW343_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9754857.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            82867..83337
                     /gene="rpsG"
                     /locus_tag="VW343_000233"
     CDS             82867..83337
                     /gene="rpsG"
                     /locus_tag="VW343_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9754858.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            83762..85843
                     /gene="fusA"
                     /locus_tag="VW343_000234"
     CDS             83762..85843
                     /gene="fusA"
                     /locus_tag="VW343_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9754859.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            85947..90338
                     /locus_tag="VW343_000235"
     CDS             85947..90338
                     /locus_tag="VW343_000235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9754860.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREVFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            90440..90703
                     /locus_tag="VW343_000236"
     CDS             90440..90703
                     /locus_tag="VW343_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9754861.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            90696..90974
                     /locus_tag="VW343_000237"
     CDS             90696..90974
                     /locus_tag="VW343_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9754862.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            90995..91147
                     /locus_tag="VW343_000238"
     CDS             90995..91147
                     /locus_tag="VW343_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754863.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            91169..92410
                     /locus_tag="VW343_000239"
     CDS             91169..92410
                     /locus_tag="VW343_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9754864.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            92420..92650
                     /locus_tag="VW343_000240"
     CDS             92420..92650
                     /locus_tag="VW343_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9754865.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            92873..93595
                     /locus_tag="VW343_000241"
     CDS             92873..93595
                     /locus_tag="VW343_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9754866.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            93812..95146
                     /gene="pepC"
                     /locus_tag="VW343_000242"
     CDS             93812..95146
                     /gene="pepC"
                     /locus_tag="VW343_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9754867.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQTKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(95202..96113)
                     /locus_tag="VW343_000243"
     CDS             complement(95202..96113)
                     /locus_tag="VW343_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9754868.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(96137..96940)
                     /locus_tag="VW343_000244"
     CDS             complement(96137..96940)
                     /locus_tag="VW343_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9754869.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(96968..97957)
                     /gene="manX"
                     /locus_tag="VW343_000245"
     CDS             complement(96968..97957)
                     /gene="manX"
                     /locus_tag="VW343_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9754870.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT        29313 a        17975 c        21715 g        29025 t
ORIGIN      
        1 tctttgataa aattagatca aatgggatta gaattgacaa atgggggaat aatagaagtg
       61 tttattccta atcatttgag aaagttgaaa aattttattg aagaggaatt caataaattt
      121 agaaattctc acggagctaa tctaagcctc tatgaatact gtttgcttga taattcttta
      181 actttaaaaa atgattggaa ctattctgat ttagttatga aatttacgag taatttttat
      241 gctgatataa aagacttgtt catggaaaat tctgatattg aaatcatcca tgaagaggga
      301 gtaccttttg tatttttaga tttaataggt gaaggtaaaa aagaatatga aatgtttttt
      361 caatggttaa acttctttta caaacagctt ggaatcacat tgtatgctag aaatagtttt
      421 gggtttcgga atctaacagt agagtatttt ggaattattg ggacagaaag atatatattt
      481 aagatttgtc caggtgttta taaagggtta agttattatt tgatgaaatt tttattaaaa
      541 tctttttcaa atgaatattt aaaaactact gatgaggtta atagatgaaa aatttgataa
      601 agttgctaat aattagattg attgttaact tagcagacag tgtattttat atagtagcat
      661 tgtggcacgt tagcaataat tattcttcga gcatgttctt aggaatattt attgcagtaa
      721 attatctacc ggatttgtta ctaatctttt ttggaccagt tattgacaga gtaaatccgc
      781 aaaaaattct tataatatca attttggttc aattagcagt ggctgtaata tttttattat
      841 tattaaacca aatatcattt tgggtgataa tgagtctagt gtttatttca gtaatggcta
      901 gctccataag ttacgtgata gaagatgtgt tgattcctca agtggtagaa tatgataaga
      961 ttgtatttgc aaattctctt tttagtattt cgtataaagt attagattct atttttaatt
     1021 cattcgcatc atttttacag gtggcagtag gatttatttt attggttaag atagatatag
     1081 gcatattttt acttgctcta tttatattgt tgttgttaaa atttagaact agcaatgcga
     1141 atatagaaaa cttctctttc aaatattaca agagagaagt gttgcaaggt acaaagttta
     1201 ttttaaataa taaattatta tttaaaacca gtatttcttt aacgcttata aacttttttt
     1261 attcatttca gacagtagtt gtaccgattt tttctattcg atattttgat ggtccgattt
     1321 tttatggtat ttttttaact attgctggtt tgggtggtat attgggaaat atgctagcgc
     1381 caatcgtaat aaaatattta aaatcgaatc aaattgttgg tgtatttctt tttttgaacg
     1441 gctcaagttg gttagtagca attgttataa aagactatac tttatcactt attttatttt
     1501 tcgtttgttt tatgtctaaa ggagtcttca atattatttt taattcgttg taccaacaaa
     1561 tacctccaca tcaacttctt ggtagggtaa atactaccat tgattctatt atttcttttg
     1621 gaatgccaat tggtagttta gttgcaggaa cgcttattga tttgaatatt gaattagtgt
     1681 taattgctat tagcatacct tattttttgt tttcttatat tttttatacg gataatggat
     1741 tgaaagaatt tagtatatat tagaaatgtt tatgttcatt caaaagcata atgactataa
     1801 ctgaaaaaga aaagtgatat ctttaaggtt gttcttcttg gtggtgagat tcgtgagaca
     1861 acccaagctt ttgtcggaaa gattaccaat gctttgatgg ataggatgta ctttagcaag
     1921 atgtttttag tggtaacggt atcgtggatg gacgtgtaat aacctcttct ttcgaggagt
     1981 attttactaa aaaactagcc ttggagcgtt ccccagaaac ggacttactc attgactctt
     2041 caaagatttg gggagaagat tttgcttcat ctgttccttg aaaaaagtca cagcagtcat
     2101 cacagacgat agtactgaac aaaactatga agagttagaa atttatacgc aggtgattgt
     2161 ataaaggatc tggaaataga taagaagttg attagtattg acctaggtgg tacaaatatt
     2221 aagattactg ttctttcaaa tgacggtgag attgaaactt tgtggagtat tacaacagat
     2281 acaagtgaga aaggttctca aattatatcg gacatcatca gttctattaa aaataaattg
     2341 accgaacgga atattcctga tagcgacctt cttggaatcg gtatgggaag ttgctcatca
     2401 tactttcctt gtaaatcata ggggctataa actctccgtc tacttgtcct gcaacaattg
     2461 aagtctgctc aaaacgccgt ccgctaatct tttcatagac tttctccctt ttaggagcct
     2521 agctttctag tttgttcttt gatttttatt gagtatacca ctattttact ccctctggca
     2581 agggactttg tctatgtgga gggattgggc tcctatgtgg tggagctttt ctgttctttc
     2641 tgaaatatgg tataatagca ctaatcaatt tctaggaaaa tagatacaga aaggggctga
     2701 aagatgtctc atattattga attgccagag atgctggcaa accaaatcgc ggctggagag
     2761 gtcattgaac gtcctgccag tgtggtcaaa gagttggtag aaaatgccat tgacgcgggc
     2821 tctagtcaga ttatcattga gattgaggaa gctggtctca agaaggttca aatcacggat
     2881 aacggtcatg gaattgccca cgatgaggtg gagttggccc tgcgtcgcca tgcgaccagt
     2941 aagataaaaa atcaagcaga tctctttcgg attcggacgc ttggttttcg tggtgaagcc
     3001 ttgccttcta ttgcgtctgt tagtgtcttg actctgttaa cggcggtgga tggtgctagt
     3061 catggaacca agttagtcgc gcgtgggggt gaagttgagg aagtcatccc agcgactagt
     3121 cctgtgggaa ccaaggtttg tgtggaggat ctctttttca acacgcctgc ccgtctcaag
     3181 tatatgaaga gccagcaagc ggagttgtct catatcattg atattgtcaa ccgtctgggc
     3241 ttggcccatc ctgagatttc ttttagcttg attagtgatg gcaaggaaat gacgcggaca
     3301 gcagggactg gtcaactgcg ccaagcaatc gcaggaattt acggtttggt cagtgccaag
     3361 aagatgattg aaattgagaa ctctgaccta gatttcgaaa tttcaggttt tgtgtccttg
     3421 cctgagttga ctcgggctaa ccgcaattat atcagcctct tcatcaatgg ccgttatatt
     3481 aagaacttcc tgctcaatcg tgctattttg gatggttttg gaagcaagct tatggttgga
     3541 cgttttccac tggctgtcat tcacatccat atcgaccctt atctagcgga tgtcaatgtg
     3601 catccaacta agcaagaggt gcggatttcc aaggaaaaag aactgatgac tctggtttca
     3661 gaagctattg caaatagtct caaggaacaa accttgattc cagatgcctt ggaaaatctt
     3721 gccaaatcga ccgtgcgcaa tcgtgagaag gtggagcaaa ctattctccc actcaaagaa
     3781 aatacgctct actatgagaa aactgagccg tcaagaccta gtcaaactga agtagctgat
     3841 tatcaggtag aattgactga tgaagggcag gatttgaccc tgtttgccaa ggaaaccttg
     3901 gaccgattga ccaagccagc aaaactgcat tttgcagaga gaaagcctgc taactacgac
     3961 cagctagacc atccagagtt agatcttgct agcatcgata aggcttatga caaactggag
     4021 cgagaagaag catccagctt cccagagttg gagtttttcg gacaaatgca cgggacttat
     4081 ctctttgccc aagggcgaga tggactttac atcatagatc agcacgctgc tcaggaacgg
     4141 gtcaagtacg aggagtaccg tgaaagcatt ggcaatgttg accagagcca gcagcaactc
     4201 ctagtgccct atatctttga atttcctgcg gatgatgccc tgcgtctcaa ggaaagaatg
     4261 cctctcttag aggaagtggg cgtctttcta gcagagtacg gagaaaatca atttattcta
     4321 cgtgaacatc ctatttggat ggcagaagaa gagattgaat caggcatcta tgagatgtgc
     4381 gacatgctcc ttttgaccaa ggaagtttct atcaagaaat accgagcaga gctggctatc
     4441 atgatgtctt gcaagcgatc tatcaaggcc aatcatcgta ttgatgatca ttcagctaga
     4501 caactcctct atcagctttc tcaatgtgac aatccctata actgtcctca cggacgtcct
     4561 gttttggtgc attttaccaa gtcggatatg gaaaagatgt tccgacgtat tcaggaaaat
     4621 cacaccagtc tccgtgagtt ggggaaatat taaaagtata aaaaagtctg ggaaaaattt
     4681 tcaaaatcag aaaaacgcat aaaatcaggt gttcaaaaac cttgatttta tgcgttttat
     4741 catagaaata tttacttcat tttttcctaa ttcttttcga aactcttttt aaacgacgtc
     4801 agttttatca gtaatctcaa aacagtgttt tgagctaatt ttgccagttt tgtctgtaac
     4861 atcgaagttg tgttttacca ctctgcgact ggtttcctag tttgctctat gattttcaca
     4921 gagtattaaa ttgcgatttt gccaagtttc tttattcgtc taaaagtaga gtctgttcta
     4981 tgcgtctaat gtacgaatta ggttgaccat ttcaatagct ccttgtgcac actcagaacc
     5041 cttatttcct gctttagtgc cagctcgttc tatggcttgt tcaattgtat ctgtcgttag
     5101 cacaccaaac ataacaggaa tttcgctatt taaactgatt tgggcgattc ccttagatac
     5161 ctcgctacat acataatcat aatgacttgt attccctcta atgacagctc ccaagcagat
     5221 aattgcatca tattttttac tttttgccat ttttgatgca atcagtggta tttcaaaagc
     5281 tcctggaatc caggctaccg ctatatcttt ctcgtttaca ttctctcttt tgagattatc
     5341 tagtgctcca gataataatt ttgaagttat aaattcatta aatctcgcta caacaatacc
     5401 tattttaata ttgtttgcta ctaaattacc ttcataagtg ttcatttatt tttcctccat
     5461 atttaaaatg tgacccattc gatttttctt tgtttctaaa taaaaactat cgtaaggatt
     5521 ggcttctatt tcgattgata ttctactgga aatggtaatt ccatattttt ctaactgttc
     5581 aaccttgtca ggattatttg tcagtaaatg aagtgactga agtcccagat ctttaagcat
     5641 ttgtgctcca atatgatatt ctcttaaatc accttcaaag cctaatgcaa gattggcatc
     5701 aagcgtatcc atgccttgat cttgtaaatg ataggctttt aatttattga taagtccaat
     5761 tcctcgtccc tcctgtcgca agtaaagtaa gacacccgaa ccattctcaa caatcatttt
     5821 catagcttta tcgaattgct gtccacaatc gcaacgtaaa gagcctaaaa catcccctgt
     5881 taaacattcg gagtggaccc gacataatac attggcttca tcctctatgt ttcccataat
     5941 aagagcaaga tgatgttccc catttagttt atctatatag ctaattgctt tgaaattacc
     6001 gtatctagta ggcatattga cagttgaaac tcgttctacc agctgatcat atacttttct
     6061 atattcttgt aattctttga tggtaattag tggaatgttg tgtttcttcg agaactgaat
     6121 taaatcatct gttctcatca ttttgccatc atgattcatt atttcacaac ataggccaca
     6181 ctcttttagt ccagctaatt ttaataaatc aacagttgct tctgtgtgtc catttctttc
     6241 tagaacacca cctttttttg caattaaagg aaacatgtgt cctggcctgc gaaaatcaga
     6301 gggtgttata tcttcagcta cacacatacg tgcggtcagt cctctttcct cggcagaaat
     6361 acctgtggtc gtttctttat aatcaattga aactgtaaaa gcagtcttat gattatctgt
     6421 attgttttca accataggtg aaagcattaa ttgattagct aaactttcgc tcataggcat
     6481 acaaattaat cctttggcat aagtagccat aaaattaaca ttttctgttg tagctgcttg
     6541 tgcagaacaa attaagtctc cttcattttc tctatccttg tcgtctataa caagaacaag
     6601 tcgtcccttc tgcaatgctt ctaatgcttc ttgtattttt cgatattcca ttgactgatt
     6661 atcctttctg ctaaaatcca ttttgatata atagttcctt agatatttct gattttggag
     6721 agttatccat cagtttttgc acatatttac ctaagatatc attttcaaga tttactgtac
     6781 tcccgacttg tttactctta agaatggttt gttccaaggt atgagggata acagatactg
     6841 aaaagtttac tttggagact ttagcgacag tcagactaat gccgtcaatt gtaatagatc
     6901 ctttttcaac tattaaatct aaaatttctt tttgtgtgtt gatttgatac catacagcat
     6961 tatcatcttt ttttattgac gagatttttc ctgtaccatc aatgtgtcct gtaacgacgt
     7021 gacccccaag tcgaccgttg acagataagg ctctttctag attcacctca cttccatgtt
     7081 ttaatagagt aagagctgtt cgactccatg tttcattcat tacatcaact gtaaaggatt
     7141 gatggttgaa atgagtaact gtaagacaga taccatttac tgctatacta tcgcctaaat
     7201 ggatatccgt taatattttt gaggctttaa ttgatagttt acaattacga gagtctttct
     7261 gtattctttc aacttttccg atttcttcaa ttattcctgt gaacatggat aaatcacttc
     7321 actttctatg agatagtcat ttcctatttg agaaaaagca taaggtttca atctaatagc
     7381 gtcatttggc aaagaaatgc cttcacctcc gacaggaaac ttggcactgc ctccaaaaat
     7441 ttttggtgca atatatattt tcagctcatc aacaatttgt tgttccaaag cactccaatt
     7501 cattagactg cccccttcta gaactaggct atcaatctgc atgtttccta gatgttgcat
     7561 taaactcgat aagtctatat gattgccttt tttctttatg gaaagtattt cacagccatg
     7621 attttgatat agcttcattt tatttttgtc ttcagaggaa gtggcaatgt aagttttaat
     7681 atcatttgct gtttttacga ttttagaggt aagaggagtt cgtaaatgtg tatcgcatat
     7741 gatacggata ggatttttcc cttcctccaa tctacatgtc agcaaaggat cgtcttgaat
     7801 aacagtattg actcccacca taattgcact aacatagtgt cgtaactgat gcacatgctt
     7861 tcttgcttct tcttcagtaa tccatttgga ttgatttgtt ttagtggcta tttttccatc
     7921 cattgacatt gcatatttca taaaaacata gggtacatgc tgggtaatat actttctaaa
     7981 actttttatt aagttaagac actcattttc taaaattcca acagtaactt gaagattatt
     8041 ttcctcaagt atctttactc cttttccaga tacaatagga ttacagtcta ggcttccaat
     8101 gactactctt gtaataccac tatcgattat agcatctata cagggaggtg ttttcccgaa
     8161 gtgacaacag ggttcaagtg ttacataaag cgtcgctccg acaggggatt ctctacagtt
     8221 tttaagagca tttctctcag catgtgggcc accaaaaaac tcatgataac cttgtccgat
     8281 gatgtgatta tcttttacaa taactgcgcc gaccatagga ttgggattga cgtaaccagc
     8341 ccctttttgt gccagtttta ttgctaattt catatatttt gaatcgctca tctcactacc
     8401 tccaaaaaaa atataccttg aataggggac tactcaaggc atacaaaaga aaacttatgc
     8461 gattaacaaa aatactctga aatgacaagt aatcatttca gagcacgcaa aaagcacaaa
     8521 tatactttta tcttctttca tccagactat actgtcggct ttggaatttc accaaatcat
     8581 gcctttcggc tcgtgggcta taccaccggt agggaattac accctgccct gaagatagtt
     8641 attcaattac agatgattat agtacttaat tttgaatatg tcaacagata aataccgatt
     8701 gtttttgata tactgtattt gtgataatcg attctcgctc ctcggataaa gaaaatatga
     8761 tatactagat aaacgaaata agagagaagg aatactatgt acgcatattt aaaaggaatc
     8821 attaccaaaa ttactgccaa atacattgtt cttgaaacca atggtattgg ttatatcctg
     8881 catgtggcca atccttatgc ctattcaggt caggttaatc aggaggctca gatttatgtg
     8941 catcaggttg tgcgtgagga cgcccatttg ctttatggat ttcgctcaga ggatgagaaa
     9001 aagctctttc ttagtctgat ttcggtctct gggattggtc ctgtatcagc tcttgctatt
     9061 atcgctgctg atgacaatgc tggcttggtt caagccattg aaaccaagaa catcacctac
     9121 ttgaccaagt tccctaaaat tggcaagaaa acagcccagc agatggtgct ggacttggaa
     9181 ggcaaggtag tagttgcagg agatggcctt cctgccaagg tcgcagtgca agcaagtgct
     9241 gaaaaccaag aattggaaga agctatggaa gccatgttgg ctctgggcta caaggcaaca
     9301 gagctcaaga aaatcaagaa attctttgaa ggaacgacag atacagctga gaactatatc
     9361 aagtcggccc ttaaaatgtt ggtcaaatag gagcagagaa tgacaaaacg ttgttcgtgg
     9421 gtcaagatga ccaacccgct ctacatcgcc tatcatgatg aggagtgggg tcagcccctc
     9481 catgatgacc aagcattgtt tgagttgttg tgtatggaaa cctatcaggc aggcctgtct
     9541 tgggaaacgg tactcaacaa acgccaagct ttccgagaag cctttcatag ctatcaaatt
     9601 cactcagtcg cagagatgac tgacactgaa ttggaagcca tgctggagaa tccagctatc
     9661 attcgaaata gagccaagat ttttgctaca cgcgctaacg cccaagcctt tctacagtta
     9721 caggcagagt acggctcttt tgatgcctat ctttggtctt ttgttgaggg gaaaactgtc
     9781 gttaacgatg ttcctgatta tcgccaagcc ccagctaaaa cacccttatc tgagaaatta
     9841 gccaaagatc tcaaaaaacg aggcttcaag ttcacaggcc cagtcgccgt attgtctttt
     9901 ctacaggctg cagggctagt tgatgaccac gagaatgatt gtgagtggaa aggtcttaaa
     9961 tgatgtctaa caaaaataag ggaattctga tttttgcgat tctctataca gtcctctttg
    10021 tgtttgatgg cgttaaattg ctggcttctt taatgccatc tgccattgca acttatcttg
    10081 tttatgtagt tttagcttta tatggctcct tcttgttcaa ggatagattg atccaacaat
    10141 ggaaggagat tagaaagact aaaagaaaat tcttctttgg agtcttaaca ggatggctct
    10201 ttctcattct gatgactgtt gtctttgaat ttgtatcaga gatgttgaag cagtttgtgg
    10261 gactagatgg acaaggtcta aatcagtcta atattcaaag tacctttcaa gaacaaccac
    10321 tactgatagc tgtttttgct tgtgtcattg gacctctggt agaagaatta tttttccgtc
    10381 aggtcttatt gcattacttg caggaacggt tgccaggttt actaagcatt attctggtag
    10441 gacttgtttt tgctctgact catatgcaca gtttggctct atcagagtgg attggtgcag
    10501 ttgattactt aggtggaggc cttgcctttt ctattattta tgtgaaagaa aaagagaata
    10561 tctactatcc cctacttgtt cacatgttaa gcaacagcct ctccttaatc attttagcta
    10621 tcagtatagt aaaatgaaat aagaacagga caaatcaatc aggacagtca aatcgatttc
    10681 taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatacactgt aacatgtgat
    10741 gaaaatgcca gtaatgatac cgagaaaaaa gctgagaaac ttttcccagc tttatttgtt
    10801 atagtcaaag cgaatgactt gttcctgtgc atctacatga gcatggaccc caaagggtac
    10861 aattgctctt ggagttgcgt ggccgacatt cagattatag acaatcggga tattgttgtc
    10921 aatgatatcc aatagtgcct ctttatagtc gtcatggaaa gtttcatcca taggttttcc
    10981 gaccaagagt ccattgatga ccgcgaatat gccagtgtcc tttaaagtta gcaacatctt
    11041 tttgaagtct tctggcttag gctcttcttc gcttgtttcg agcaagagga ttttcccttc
    11101 ccagtctgac aagtcaggga aaagtttgta tttttggcag agttccgtgc tatctgcgta
    11161 tcgagagttg tcaaagatat cgtagaggga ttcgaggcaa ccaccgagga ttttcccctc
    11221 gaactgagca cttccttgca acaagtcaaa acctgtattt gtatgactga cacgaggtgt
    11281 tcccagggcc gtgggactaa aatcagttcg ttcctcatac caaacgtcac tagggcggat
    11341 ttctgaaatt cttcccgtct caatcaattc tttaaagtag tgaaggctat aggctagcat
    11401 ttctttgtct aattcacaaa tgtctgctaa aaaggattga ccataaaaag tcttgattcc
    11461 taatttatgc aacatgaggt ggttcatggt tgtatccgag aagccaagaa aaattttttg
    11521 tttgataacc ttttggagtt ggtcattttc aaaaagataa ggtagcaagc gataggtatc
    11581 gtctccacct atggcacata ggatcatgtc gatgctatca tcagaaaagg catgaatcaa
    11641 atcctctgca cgagcttcag gatggtcctt gataaagtct aatcctttta acgaatgggg
    11701 caaaaagatg ggattgagtc ccagaacctt gagacgttgg atacccaagt ccacttcgtg
    11761 tttgacaaaa tcctctccga taatgccact agataaacta acaataccaa tagtagaaac
    11821 catatctcat cctcctagaa atagattgag cctattttat cacaaaatat aagagaaagc
    11881 tatgtgggat ttcagaagaa tacttttaat actcttcgaa aatctcttca aaccacgtca
    11941 gttttatctg caacctcaaa gctgtgcttt gagcaacctg cagctagctt cctagtttgc
    12001 tctttgattt tcattgagta taactcaaaa aagtaagtga aaaagcaacc gcacctgcag
    12061 ttgcttttat cgttttctta ttcttgatag cgtgttggtc ctgcacttgc actacggata
    12121 ttcaagcgtt tcttgagata gaagcgaagg gctaggagag ctgctccaat aatagctagt
    12181 ggcaatggat ccagtactgg gttaaggcta gctggtagga agcttgttgc aaagaagaca
    12241 agcaaccaaa ggaacataga agctaggata actagtacag atttccagaa aggtggacgt
    12301 tgactgcgat ccatatctgg tccatagtat tggtaaacaa agtagtacat caagtagaag
    12361 gcaaatccac caaccagtcc aactaataga agagtaatca atccatagcc gaaagcttga
    12421 tctgccgcaa agaaggttgt gagggcgctg acaagggcaa agaggctagt gatgaaaaga
    12481 gctgagtcca taatcatcag ttttgggtca tcattttctt ttggatgctc tttttcgtac
    12541 tgctctttga cagtgaagct atgagcccaa tgagttggtg cgccgtatag ggaacgggca
    12601 gttgtacctt tagattgctc ctcaaggatt tggggaatga cttcctcaaa aatagtctgg
    12661 atttcagcgt ctgttttccc atctttgatg aattgttggg tagcaatgtg gacaaactct
    12721 tggtttttct tagttaattt ttgtagatca atttgagaca taggaactcc tcttagaacc
    12781 attttttatg gatgagatag agagtgagcg agacactcat agcaaaggcg ataaagacga
    12841 ttaaccagaa ggcatttggc tctccgttta ggggaatttc attatcctta aagttcatcc
    12901 cgtaggcaga aaagaccatg gttgggatgg acatgacgat ggtcacaagg gccaaggttt
    12961 tcatgatgtt gttctggttg ttagaaatga tagaggcaaa ggtctctgtc atagaatgca
    13021 agacgtttcc ataaatatct gccatctcga tggcctgttg ggtttcaatc agggtgtctt
    13081 caagcaggtc ttcgtcctca aggtatttct tgatattgct ggttgaactg gtcaatttct
    13141 taatcacgcg ctcatttgtt ttgagggagg ccttgaaata gacgatagtt ttttccaatt
    13201 ccatgagctc aatcaattct tcattacgag ttgattgatg cagttgactt tcgatttgtt
    13261 cactcttgcg gtcgattgaa cgaagggctg ttaggtaaag ctctgcattg cgataaagaa
    13321 tttgaaagat aaaacgtgaa cgcatgaagg tatagaaatt acgcaatcga cggttgataa
    13381 agacatcaag gacaggtagt ggttccaaac acgtagtgat aatggtttcc tcagtgatga
    13441 taataccaag cgggatggtt acgtagtagg tgcggttatt tctttcctcc gtgaccggca
    13501 cgtctacgat aatcagggta tactcgtctt caatggtaat acgagacatt tcttccgcat
    13561 cgagcggtgc tcgaaggtcg gcaatatcaa tatcgaaggt gttggcgatt tcgagtgatt
    13621 cattttgagt cggattgacg agattgatcc aagtacccgg ttcaagcgta tcgatctctt
    13681 taaattcagt tgttgtagag agaaaaactt gtttcatatc ccttatcctt tctcattttt
    13741 cagatttttt cacactgtac tattatacta caaaatcggc cttttgggta atagaatctc
    13801 actttttttg gtataatggt aagcaataat ggactagaaa gaacaaagat gcaagataaa
    13861 attgtcattc atggggcgcg tgcccataat ttaaaaaata ttgatgtgga gattccgcga
    13921 gacaagttgg ttgtcgtgac tggcttgtca ggttcaggga agtccagtct ggcttttgat
    13981 actctctatg cggagggaca acgtcgctat gtagagagtt tgtctgccta cgctcgtcag
    14041 ttcttgggaa atatggaaaa accagatgta gatgccattg atggtctcag cccagctatt
    14101 tccatcgacc agaaaacgac tagtaaaaat cctcgctcga cggtgggaac gacgactgaa
    14161 atcaatgact atctgcgtct cctctacgca cgtgtgggga cgccttactg tatcaacgga
    14221 catggagcta tcaatgcttc ttctgtggag caaatcgttg ataaggtttt ggagttacct
    14281 gaacgccagc gcttgcagat cttggctcct gtcatccgca agaaaaaagg ccaacataag
    14341 agtgttatcg agaaggttca gaaagacggg tatgttcgtg ttcgtgtgga tggggaagtc
    14401 tatgatgtga ccgaagtgcc agagttgtct aagagcaagc aacacaatat cgatgtcgtg
    14461 gttgaccgta ttgttatcaa ggagggcatt cgtagccgtc tctttgattc cattgaagct
    14521 gcccttcgta tcgcagaagg ttatgtgatt atcgacacca tggacgactc tgagttgttg
    14581 ttctctgagc attatgcctg tccagtttgt ggatttactg tcccagagtt agaacctcgt
    14641 ctcttctctt tcaatgctcc ctttggctct tgtagtgagt gtgacggctt ggggatcaag
    14701 ctggaggtag atactgattt ggtagtgcca gatgccagca aaaccttacg tgagggagcg
    14761 ctagcacctt ggaatcctat ctcatccaac tactatccaa acatgttaga gcaggccatg
    14821 aaagtctttg gagtggctat ggataagcct tttgaggacc tgtcagaaga agataagaac
    14881 ttgattctct atgggtcaga tggtaaggaa ttccatttcc actatgagaa tgaatttggt
    14941 ggcgtgcgcg atatcgacat tccgtttgag ggagttatca ataatatcaa acgtcgttac
    15001 catgaaacca atagcgatta cactcgcact cagatgcgtc tctacatgaa tgagctgacc
    15061 tgcggaacct gtcaaggcta tcgtctcaat gaccaggcct tgtctgtccg tgtgggcggc
    15121 cagcaagggc cacatattgg agaaatctca gacctgtcta tcgctgacca cttggacttg
    15181 gtgagccagt taactttgtc tgaaaatgaa gccatcattg ctcgtcccat tctcaaggaa
    15241 atcaaggatc gtttgacctt ccttaataat gtgggtctta actatctaac cctgtcacgt
    15301 tcagcaggaa ccctttcagg tggggaaagt cagcgcattc gtttggcaac ccagattggt
    15361 tccaacctat caggcgtcct ctatatccta gacgagccgt caatcggtct tcaccagagg
    15421 gacaatgacc gcctgattgc cagtctgaaa aagatgcgtg acttgggcaa tactcttatc
    15481 gtggtggaac acgacgaaga taccatgcgt gaagctgatt atctgattga cgttggtcct
    15541 ggtgccggtg tttttggtgg tgagattgtt gcagcaggta cacctaagca agtggctcgc
    15601 aacagcaagt ctatcacagg tcagtacttg tcaggcaaac gtgtcatccc agtaccagaa
    15661 gagcgccgtg tcggaaatgg tcgttttatt gaagtgacag gagcgcgtga gaacaacttg
    15721 caaaatgtca cggctcgctt tccactagga aaatttatcg cagtgacagg tgtgtcgggt
    15781 tcagggaaat cgactctaat caacagcatt ctcaaaaaag ccattgccca gaagctcaac
    15841 cgtaattcag acaaacctgg taagtttaag actattacag ggattgagca tgtcgaccgt
    15901 ttgattgata ttgaccagag ccctatcgga cggacgccga ggtctaaccc agctacctat
    15961 acaggagttt ttgacgatat acgtgacctt tttgctcaga caaatgaagc caagattcga
    16021 ggctacaaga aggggcgctt cagtttcaac gtcaagggag gtcgctgtga agcttgctca
    16081 ggtgacggga ttatcaagat tgagatgcac ttcttgccag atgtttatgt ggcttgtgaa
    16141 gtttgccacg gaacccgcta taacagtgaa accctagaag ttcactacaa ggaaaagaat
    16201 atttcgcagg tcttggatat gacggtcaac gatgcggtag aatttttcca acacattccg
    16261 aaaattcaac gcaaactcca gaccatcaag gatgtagggc taggctatgt gaccttggga
    16321 cagccagcta ccaccctttc tgggggagaa gcccaacgta tgaagttagc tagcgaactc
    16381 cacaaacgct cgacaggaaa atctttctac attctggatg agccgacgac agggcttcat
    16441 acagaggaca ttgctcgcct gctcaaggtt ttagctcgct ttgtagacga tggcaataca
    16501 gtcctcgtca tcgagcacaa tctggatgtt atcaagacgg cagaccatat cattgacttg
    16561 ggacctgagg gcggtgtcgg tggtggaacc atcatcgtaa caggaactcc agaagaagta
    16621 gctgccaacg aagccagcta tacaggacac tatttgaaag gaaagttaca tcatgaataa
    16681 acgcgtacaa gcatttctag ctaaaatgca agaaaaagaa ctagatggta tcatcatcaa
    16741 caatcttaaa aacgtctatt atttgactgg tttttggggc tcaaacggaa cagtctttat
    16801 cagtcgtgac cgtcaggtct tagtgacaga ctctcgctat atcatcgcag ctaagcaaga
    16861 aaccagtggt tttgagattg tggctgatcg tgatgaattg gctgtcattg caggaattgt
    16921 taaggacatg ggcttgactc gtatcggttt tgaagatgag atttcagtgt cttattacca
    16981 ccgtatgcag gcagcttttg caggtttgga cttgtttccg caaactcagt ttgtggaagg
    17041 tcttcgtatg attaaggatg aggcagagat tgcagcgatt cgcaaggctt gttctatctc
    17101 agaccaagct ttccgcgatg cgcttgactt tatcaaacca ggaaaaactg aaattgagat
    17161 tgccaacttc cttgacttcc gcatgcgtga gttgggagca tctggcttat cttttgatac
    17221 gatcctagct agcggtatca attcttctaa accccatgcc catccaatgc acaaaccagt
    17281 ggagttggga gaagccatta ccatggactt cggctgtctc tatgaccact atgtcagtga
    17341 tatgacccgg actatctatc tagggcatgt tagcgatgag caggcagaga tttacaatac
    17401 ggttctaaaa gctaaccaag ccttgattga ccaagctaag gcaggcttag gtttccgtga
    17461 ctttgacaaa atccctcgtg atattatcat tgaggcaggt tatggcgact actttactca
    17521 cggcattggc cacggtattg gtctggatat ccatgaggaa ccctacttta gccagacttc
    17581 tacagaaact attaagacag gtatggcctt gaccgatgaa ccaggtatct atatcgaagg
    17641 caaatatggc gttcgtatcg aggatgatat cctgattaca gagacaggtt gtgaattatt
    17701 gaccctagct ccaaaagagt tgatagtcat ttagttgttt gtcaagaaaa aagtacgaaa
    17761 atgacgaaaa aagtcaaaaa aaattaaaaa taggtcgcaa gtcggatgtt ttttatggta
    17821 taatagacta aactgatagt aacatgtagc gaaaggggta ggtacatgat taaaatttat
    17881 acagtctcaa gttgtactag ctgtaaaaaa gcaaaaacct ggctcaatgc ccaccagtta
    17941 agttataaag aacaaaacct tggtaaagaa ggaattacga gagaagaatt actggatatt
    18001 ctaaccaaaa cagataacgg aatagccagc atcgtttcgt ctaaaaatcg ctatgccaaa
    18061 gcccttggag tggatattga agatttgagt gtcaatgaag ttctcaatct gattatggaa
    18121 acaccgagaa ttttaaagag cccaatcctt gtagatgaaa aacgcctgca agttggctac
    18181 aaggaagacg atattcgtgc cttcctacca cgctctgtcc gtaatgtaga aaatgcagaa
    18241 gcacgtttac gtgcagctct ataaacatca aggctgggag caattcccag tcttattcta
    18301 ttttaatttc aaaaagaaag aagaaagaaa tgaaaaaaat agttcttgtt agtctagctt
    18361 tcctttttgt cctggttggt tgcggacaga aaaaagaaac tggaccagct acaaaaacag
    18421 aaaaggatac gcttcagtcg gcattgccag ttattgaaaa tgccgagaag aatacagttg
    18481 taaccaagac tttggtcttg cccaagtcag atgatggtag ccagcaaact caaaccatta
    18541 catacaagga caagactttt ttgagcttaa cgattcaaca aaaacgtcca gtttctgatg
    18601 agttgaagac ttatattgac caacatggag tggaagaaac tcaaaaagct cttcttgaag
    18661 cggaggagaa ggataagtct atcattgaag ctcgtaaatt ggtaggtttc aaacttgaaa
    18721 caaaactatt gagcgcaacg gaacttcaaa caacgactag ttttgatttt caagttctgg
    18781 atgtcaagaa ggcttcccag ttggaacatc tgaagaatat tggtttggaa aatcttttga
    18841 aaaatgaacc aagcaaatat atttcagaca gattggcaaa tggtgcgaca gaacagtaga
    18901 aaatccaaat aaatagactg cctgaattgt agaaggtaag gaattatgct ataatggtac
    18961 tattctaagg aaagagggtg ttagaatggg atttactgaa gaaacagtac gttttaaatt
    19021 ggacgattcc aataaaaaag aaattagcga aactttgaca gatgtttatg cttcgttgaa
    19081 cgataagggt tacaacccaa ttaaccaaat cgtaggttac gtattgagtg gagaccctgc
    19141 ctacgttcct cgttataata atgcacgaaa tcaaatccgt aagtatgagc gtgatgaaat
    19201 cgttgaggaa ttggttcgct actacctcaa aggacaagga gtcgatctat aacctatgag
    19261 aattatggga ttggacgtcg gttcaaaaac ggtaggggtg gcgattagcg atccgcttgg
    19321 ttttacagct caagggcttg aaatcatcca gataaatgaa gaacaaggcc aatttggttt
    19381 tgaccgcgtt aaggaattgg ttgatactta caaggtggaa cgatttgtag tgggcttgcc
    19441 taaaaacatg aacaatacaa gtggaccgcg cgtagaagct agtcaagcct acggagcaaa
    19501 gctagaagag ttttttggtt taccagtaga ctatcaggat gaacgcttga caacagtggc
    19561 tgctgagcgc atgttgattg aacaagcaga tatcagtcgc aataagcgca agaaagtcat
    19621 tgataagtta gcagctcagc tgattttaca aaattattta gatagaaaat tttaatataa
    19681 aggagaggct atgtcacacg atcataacca cgaccacgaa gaacgtgaac taatcacact
    19741 agtagatgag caaggaaatg aaaccttgtt tgaaatcctt ttgacaattg atggaaaaga
    19801 agaatttggt aaaaactatg ttcttctagt accagttaac gcagaagaag acgaagacgg
    19861 acaagttgaa atccaagctt actcattcat cgaaaacgaa gatggaacag aaggcgaatt
    19921 gcaaccaatc ccagaagact cagaagacga atggaacatg attgaagaag tcttcaacag
    19981 ctttatggag gagtgataca gtctgggaga ctgtatcagc ccaactccca gaaatgggga
    20041 agagttggaa cgtccagtgg atgtttttag cccagttcct ttaaataaga gagaactggt
    20101 aggttcgggg ggacgttttt agcccagctc ctttaaataa gatagagctg gtaagtctgg
    20161 gagactgtat cagcccaact cccagaaatg gggaagagtt ggaacgtcca gtgaacattt
    20221 ttaccccaac tcttagaaat tgagaagagt tggaacatct agtggatgtt tttagcccat
    20281 attcacattc atagtggcta gttattagct aaccaggtaa gaggtcggga cgaaaatcct
    20341 ggccttgttt ttattagtaa ccatacttta atgcgataat tgattggact tttgtcaagg
    20401 agatgtgtat gtttgaagta gaagaatggc tccatagtcg gattggttta aattttcgat
    20461 caggtttggg ccgaatgcag caagcggtgg atttgttagg aaatcctgag cagtcttacc
    20521 ctattatcca cgtaacaggg actaatggga aaggatctac cattgctttt atgagggaat
    20581 tatttatggg gcatggcaaa aaagttgcga cctttacctc ccctcatatc gtctctatca
    20641 atgaccgaat ctgcattaat gggcaaccta tagcagacgc agactttatc cgtttggctg
    20701 atcaggtcaa ggagatggag aaaacgcttc tgcaaactcc tgaccagttg tccttttttg
    20761 aattgctgac cttggttgct tttctttatt ttagggagca agaggtggat ttggttttat
    20821 tagaagtggg aattggtggc ttacttgaca cgaccaatgt ggtaactgga gagcttgctg
    20881 tcatcacctc cattgggctt gaccatcaag aaaccttggg tgatagtcta gaagcaattg
    20941 cagagcagaa agctggtatt ttcaaggctg gtaagaaggc agtgattgcg aaattgcctc
    21001 cagaagctag gcttgtctgt cagaaaaaag ccgaatcttt agctgttaac ctttatcagg
    21061 caggtcaaga ttttttaatg ctgaatggtg atttttcaag ctctttacta aatctttcac
    21121 agctgaacat aggcttagaa ggagtctatc agcaggagaa tgcagccttg gcgttgcaaa
    21181 cttttcttct ttttatggga gaaggaaagg aagctgttga tgaacaggct gtaagaaagg
    21241 ccttggaaca gacccattgg gctggtcgct tggagcgtat tcgcccacag atttacttgg
    21301 atggtgctca taacctccct gccttgactc gcttggttga gtttatcaaa gaaaaagagc
    21361 aggaaggcta tcgacctcaa atcctttttg gagccttgaa acggaaggat tatcaaggga
    21421 tgttaggtta cctgactgaa aaattgcctc aggtggaact caaggtgacc ggctttgact
    21481 atcagggggc tttggacgaa agggatgtga caggttacga tatagtttct tcttgccgag
    21541 aatttatcag cgattttgaa gaaagagcag atgctcagga tttgctgttc gttacagggt
    21601 ctctctattt tatctcagaa gtacggggct acctgctgga ccgtgagcag ataaattgac
    21661 ccccttgttt gaacttgtta tactagggga attgctggtt agagaaaatt tctctaaatt
    21721 ggtagcagaa aggaaattca tcatgaaatt aaaaagattc acactttctc ttgcttctct
    21781 agcaagtttt agtctcttag tagcttgttc acaaagagct caacaggttc aacagcctgt
    21841 tgctcagcag caggtccaac agcctgctca acaacctgct caacagaata ccaatactgc
    21901 aaatgcagga ggtaaccaaa atcaagcggc tccagtacaa aaccaacctg ttgctcaacc
    21961 gaccgatatt gatgggactt atactggtca ggatgacgga gaccgtatca ctttagtggt
    22021 aactggaacg actggtacat ggactgagct cgaatctgac ggggatcaga aagtcaaaca
    22081 ggttacattt gattcagcaa atcaacgcat gattattggc gatgatgtca aaatttacac
    22141 tgtaaacggt aatcaaatcg tcgtagatga tatggataga gacccatcgg accaaatcgt
    22201 tttaactaaa taagactatg caagtagaaa gggctggatt tttcagcctt tttactttta
    22261 cctagaaaat aatcataaat acttgccttc taccgaatac ctgcgttata ctagtatcaa
    22321 ttacttgttt ttagcattca aactatcaag gaggggatat gaaatatagg aaatttcaat
    22381 tattgatgtc caagtatggc tttagtcttt cgattatgtt acttgaactt tgtcttgttt
    22441 ttggtctctt tctttattta ggacgcatgg ctcccatttt atggattact gtcctcattc
    22501 tactgagtat catcacaatc atttcgatag tcaaccgtaa tacgactcct gagaataagg
    22561 taacctggtt gttagtagcc tttgtgccag tatttggtcc cttgctctat ctgatgtttg
    22621 gtgaaaggcg attgtccaaa aaagaaatca aacaactgaa gaagctaggc tctatgcatt
    22681 tccaagaagc aaatagccag ctactaaaag agaaattaaa agaaagtgac aaggcagctt
    22741 atggagtcat caagtcctta ttgagtatgg ataccaatgc tgacatctat gatcaaactg
    22801 cctctacatt ttttcctaac ggagaagcta tgtggaaaaa gatggtagaa gatcttaaaa
    22861 aggctgagaa atttattttc ttggaatatt acattataga agaaggtttg atgtggaatc
    22921 gcatactaga tatactagag caaaaggtag ctcagggtgt agaggttaag atgctctatg
    22981 atgatatcgg ctgtatggct actttaacag gagattatgc acatcgactt cgtcagctgg
    23041 gcatcgaggc ccataaattc aataaagtta ttcctcgttt gacagtggct tataataaca
    23101 gagatcatag aaaaatattg attgttgatg gtcagatagc ctatactggt gggatcaatc
    23161 tggcagatga gtacattaac cacgtcgaga gatttggtta ttggaaggat agtggaattc
    23221 gcttagacgg actagcagta aaagctctga cacgcttatt tttgaccact tggtacatta
    23281 atcgaggaga aattagtgat tttgatcaat atcatttaga aaatcattct atcccgagtg
    23341 acggtttaac cattccatac ggaagtggac ccaagccaat ttttcgagcg caggtaggga
    23401 aaaaagttta tcagagttta atcaatcaag caacagaatc ggtctatatt acgacacctt
    23461 atttgattat agattatgat ttaacagaga caatcaaaaa tgcagctatg agaggggtcg
    23521 atgttcgaat tatcacccct tacataccag ataagaagtt cattcagtta gtcacgagag
    23581 gagcttatcc cgaccttctt tctgctggtg ttcggattta tgagtatagt ccaggtttta
    23641 ttcatagtaa gcagatgttg gtagacgaag attttgcggt ggtggggaca atcaatctcg
    23701 actaccggag cttggtacac cattatgaaa atgcagtctt actctataaa actccttcta
    23761 taagggaaat cgcccgagat tttcgaaata tatttgcaga ttctcaggaa gtctatcctc
    23821 attctatcaa aacgagctgg tatcaaaagc ttgtaaaaga aatcgcccag ctattcgccc
    23881 ctatcttata agaaatctag aacttaggat acaaaccaga cctatttttc ttgtctttta
    23941 cgaatagaaa gaaagaggag gggaattcat gctaactaag gaagaagtga atgatatgat
    24001 tgagttcaaa cagacacatt tacaccgctt tcgggaaatt gaaacctttg tgaaactatg
    24061 caaaaaatca ctcaaacagc catcgcaagc tgacaatcct aggacttttt gatatactaa
    24121 gacagataaa ttgaacagga gaaacgatat gagtgtgtat ggtagagtag aagaagttca
    24181 taaggagaat cgtgaacctc tagaatacca aatcgaacaa gaatcgcatc atcgtgaatc
    24241 aagtcgtctt cctttggtga aaattttact atggagtacg cttgtaacgg ggataactct
    24301 aggagtaccg ctattactcg acttaatgag tgcacaagaa gtgcaggatt tttatgcagg
    24361 ttgggcgctt catcagacag ggaagattta cagcgactat tatggaagtc aaggtttgct
    24421 ttattatttg ctgacttacg tgagtcaggg cggatttttc tttgccatct ttgagtggtt
    24481 agccttggta gcaggaggat ttttcctttt tagatcagcg gacaccttga cagagcaagg
    24541 agaccaagct ggacatctgg tgactatttt ttacatgcta gttacaggtc ttgcttttgg
    24601 tggaggctat gcgactcttt tagcgcttcc tttcttattc gcagccttta gtttagttgc
    24661 ggcttaccta agcaatccaa gccatgataa gggatttgta cggattgggc tagctttggc
    24721 aggcggattt ttctttgctc ccttatcatc gctcctgttt attgctgtag tgagtttagg
    24781 cttgttggtc tttaaccttg ggcatagacg ctttgcgcat gggttttatc agtttcttgc
    24841 agtggcttta ggtttttcac ttgtctttta tccaactgcc tactatagtg ctgcaacagg
    24901 aagttttggg gatgcgatta gtggtattcg ttatcctatt gacagtattc gctttgattt
    24961 tacttctaaa attttagaga atatgttttt ttatggcttg ttgtcccttg gtttgggatt
    25021 tgtggtttgt atctttttag gtctctttca atccaaaccc tttaaactat acgtcatttc
    25081 ggttcctgca agtcttgtgg taattttagg tttgatttta cttttctttt cacaagagcc
    25141 tctgcacgct tcttatttga tggtcgtctt ccctgttttc ctacttttat tggtaaccaa
    25201 tattaagagt caacagaggg ggcgtagtgc tagaagaagc cgaagagaaa cgccagttag
    25261 cctatggagt cgtttcttca aaggaaatct atatctgcta gtttttgagt ttgtctatct
    25321 tttgtctgtt ccttttttga tgaagtttgt cctttatcca gtaccttatc aagaacgtaa
    25381 tcgtcttgct gatttggtaa aagaggagac aaatacggaa gatgctatct atgcatggga
    25441 tgatactgcg actctttatc gtaagagtga gcgcttgtcg ccatcggcga ttttgtcccc
    25501 gttgcactat acagcaactg aggaaaatcg taataagtta cttaatgact tgaaagaaaa
    25561 acaacctaag gtgattgtgg taaatgataa ggtggtagtc tggtctgaag tggaaacact
    25621 cttaaaagaa aattaccaac aagtaaagac tgattactca gagtttaaag tctataaaat
    25681 taaataacca aatcaatatc ttgtgtattt ttaaaaattt taggattttt aacacaagat
    25741 attgattttt ctttttagag tggtataata ctttttagaa agaacatttt agaaaagagc
    25801 atgcatatga ttgcactaga agaaaaaatt acaattttgc caactctctt cgtcgagaaa
    25861 cgagatggga gacgtgttgt atttgatgtg gacaagattg acaaggctct ccacaaggcg
    25921 gctgacaagg ttatggatgt gacacccctg gttgaaaaac gcctcaatga tctgactgag
    25981 cgaattatta cagaaattca tagtcgcttt ccacagggaa ttaagattta cgaaattcaa
    26041 aatatcgtag aacatgaact ccttgaagcc aaagaatatg cgctggctga ggagtatatt
    26101 acttatcgga cacagaggga ttttgagcgc tcaaaagcga cggatatcaa ctttagtatt
    26161 cataaacttc tcaacaaaga ccagacagtt gtcaatgaaa acgctaataa agacagtgat
    26221 gtctttaaca ctcagcgtga tttgacagca gggattgttg ggaaatcaat cggactgcaa
    26281 atgcttccta agcacgtagc caatgcccac caaaaggggg atatccacta tcacgatttg
    26341 gactacagtc cctatacccc tatgaccaac tgctgtttga ttgattttaa gggtatgttg
    26401 gaaaatggtt ttaagattgg aaatgcagag gtagagagtc ccaagtctat ccagactgcg
    26461 acagcacaga tttctcaaat cattgccaac gttgcttcta gccagtacgg tggctgttca
    26521 gctgaccgta tcgatgaaat tttggcgcct tatgcagaga agaattatca aaaacatctc
    26581 aaagatgcag aagagtgggt attgcctgaa aaacgggaag attacgcttg gaagaaaacg
    26641 caaaaggaca tctacgatgc catgcaatct cttgagtatg aaatcaatac tctcttcact
    26701 tcaaatggac aaacaccttt tacttcgtta ggttttggtc tgggaaccag tcgttttgaa
    26761 cgagaaattc aaaaagctat tttaaacatt cgcatcaagg gtcttggttc agaacaccgt
    26821 acggctatct ttcctaaact tattttcact ctgaaaagag gccttaattt agaggaagga
    26881 actcccaact acgacatcaa gcagttggct cttgagtgtg caaccaaacg gatgtatcca
    26941 gatgttttat cttacgataa gattgttgac ttgacaggat ccttcaaggt gcctatgggt
    27001 tgccgttctt tcctccaagg atggaaggat gaaaatggtg tagaagtcaa ttcaggtcgc
    27061 atgaatctgg gtgttgtgac ggttaatctg cctcgtattg ctcttgagtc tgaaggtgat
    27121 atgaataagt tctgggaaat cttcaacgag cgaatgaata tcgcagaaga tgctcttgtt
    27181 taccgtgtcg aacgcactaa agaggcgaca ccagcaaatg ctcctattct ttatcagtac
    27241 ggtgcttttg gccatcgtct aggtaaagaa gaaagtgttg accagctctt taagaatcgt
    27301 cgtgctaccg tttcgctggg ctatatcggc ttgtatgaag tagcgacagt tttctttggt
    27361 aacagctggg aaagtaatcc agatgctaag gaattcacgc tagacatcat tcacgatatg
    27421 aaacgccgtg tagaagagtg gtcagaccaa tatggctacc atttctctat ctactcaaca
    27481 ccatccgaaa gtctgacaga ccgtttctgc cgactagata tagacaagtt tggctctatt
    27541 cctgatatca cagacaagga atactacacc aactctttcc actacgatgt tcgtaaaaat
    27601 ccaacaccgt ttgaaaaatt ggactttgag aaagtctatc cagaagcagg tgcgtcaggt
    27661 ggtttcatcc attattgtga gtatccagtc cttcagcaaa atccaaaggc cttggaagct
    27721 gtctgggatt atgcttatga ccgtgtaggc tatctaggca ccaatactcc gattgaccgt
    27781 tgctacaagt gtgactttga aggggatttt gaaccaactg agagagggtt tgcttgtcca
    27841 aactgtggca atagcgaccc taaaacagta gatgtggtga agcgaacttg tggctaccta
    27901 ggtaatcctc aagcaagacc gatggtcaac gggcgtcaca aggaaatcgc tgcgcgtgtc
    27961 aaacatatga atggttcaac gattaaaata gctgggcatc aagtaacaaa ttagaaagaa
    28021 atgaaatggg aaaatatcaa ctagacgata aggggcgcgc acaagtgacc cgttatcacg
    28081 agaaacactc taaaggtgga gctggtaaga aagaacgctt gcttagcttc agagaacaat
    28141 ttttaaacaa gaacaagaaa aaataaaagt gagagccagc tctcgctttt ctcatagtgg
    28201 gaggtaagga tggaattacg cagaccaaga ttagcggata agaaagctgt tttagatatg
    28261 atgacagagt ttgaaaagag tcaatcagcc catgatggag gattttggga tgttgaggat
    28321 ttttcttacg aggagtggct agaaggtaac cgtaataaag aaatgggaat atggctacct
    28381 gaaaatcgcg ttccatcgat acagttcgta ttgtttggtg aatcaggttg tgctctagga
    28441 tttttgaatc tgcgattgag actgaatgag gggttactga attatgctgg ccacattggc
    28501 tactccatcc gtccatctga aagaggcaag ggttatgcaa aagagactct ccgtcagggc
    28561 ttgcaagttg ctaaggaaaa gaacatcaag aaagctctgg tgacctgtag cgtgaataat
    28621 cttgctagca gagcagtcat tctagcaaat ggtggaatat ttgaggatgc tcgcaatgga
    28681 gtcgagcgtt attggataga ggtagcgaat gaataatcca aaaccacaag aatggaaaag
    28741 cgaggaactt agtcaaggtc gtatcattga ctacaaggcc tttaactttg tggacggcga
    28801 aggcgtgcgt aactctctct atgtatcagg ctgtatgttt cactgcgagg gatgttataa
    28861 tgttgcgact tggtctttta atgctggcat tccctataca gcagaattag aagagcagat
    28921 catggcagat cttgctcagc cctatgttca aggtttgact ttgttgggag gggagccttt
    28981 tcttaatact gggattctct tgccacttgt taagcggatt cggaaggaat tgccagacaa
    29041 ggacatctgg tcctggaccg gctacacttg ggaagaaatg atgttggaaa ctccagataa
    29101 actggaattc ttgtcactga ttgacattct tgtcgatgga agatatgatc gaactaagag
    29161 aaatcttatg ctccagtttc gaggttcgtc taaccaacga attatcgatg tgcaaaaatc
    29221 gctcaaaagt gggcaagtag tgatttggga caagctcaat gacggaaaag aaagctatga
    29281 acaggtgaag agagaatgaa gaaaaaagac ttagtagacc aactagtctc agagatcgag
    29341 acggggaaag tcaggacact gggaatatac ggtcatggag cttcaggtaa atcaaccttt
    29401 gcacaggaat tgtaccaagc tttagattct actacagtaa atttgctaga gacagatcct
    29461 tatatcacct caggacgcca tctggtagta cccaaggacg cgccgaatca aaaggtgaca
    29521 gccagtctgc cagtggcgca tgaactggag agtttgcaga gagatatcct tgccttgcag
    29581 gcgagtatgg atgtcttgac aattgaagaa ccttggaagg ctagtgaggt cttgtctgga
    29641 gccaaaccaa ttttgattgt cgaagggatg tctgttggct ttctacccaa ggaactcttt
    29701 gaaaaaacca tctgtttcta cacggatgag gagaccgaat taaagcgacg ccttgctaga
    29761 gatacgactg tgagaaatcg cgatgcatcc tttatattgg ctagccatca gatgagacgg
    29821 gagcagtatc tgcgatacta taaagaaact gagtctaagg cggatatctt agtggaccaa
    29881 tcagaagata aatttgatgt caagaggact taacttatgt atagaagaaa tatagaagaa
    29941 aatccctaat tttagaaaga aaaagtagga aaacagtttc atccccaaaa aacgaaaaaa
    30001 cctaacaaat cccttgcaat cgcaggggat ttgtgttatt ctattatggt gctgtaaatt
    30061 acagccttag ttttgatgca agaggttgcg acacgctcgg ttgcattgcc acgcaacacc
    30121 gcgtcggttt tcttgtggag ctagcctatt atcttaaata gacgaaaagg agaaaaagat
    30181 ggcaaacaaa aaaatccgta tccgtttgaa agcttacgaa caccgtacgc ttgacacagc
    30241 ggctgcaaaa atcgtagaat cagctactcg tacaggtgca caagttgcgg gtccaatccc
    30301 acttccaact gaacgtagcc tctacacaat cattcgtgcg actcacaaat acaaagactc
    30361 tcgcgaacaa tttgaaatgc gtacacacaa acgtttgatc gatatcgtta acccaactca
    30421 aaaaacagtt gatgccttga tgaaattgga tcttccaagt ggtgtaaacg tagaaatcaa
    30481 actttaatct aaaatataaa agagcagagg ctggtgtttc aatctaattg aacacgggct
    30541 aaactcggtg tgaaaaagat aaacttcctc gcgtctgcta gacactgtgt cagtttccta
    30601 ttttcacttt gagtttgacg ccctttgtat cttagacttg agcataaaaa acgctcgtta
    30661 aaaacttttt gaataaaaaa tatagaaaag gaactatttt ctcatgacaa aaggaatctt
    30721 agggaaaaaa gtgggaatga ctcaaatctt cactgaagct ggcgaattga tccctgtaac
    30781 agttattgaa gcaactccaa acgttgttct tcaagttaaa actgttgaaa cagacggata
    30841 caacgctatc caagttggtt tcgatgacaa acgcgaagta ttgagcaaca aacctgctaa
    30901 aggacatgta gcgaaagcta acacggctcc taagcgcttc attcgtgaat tcaaaaacgt
    30961 tgaaggcttg gaagttggtg ctgaaattac agttgaaaca ttcgcagctg gagacgttgt
    31021 tgacgtaact ggtacttcta aaggtaaagg tttccaaggt gttatcaaac gccacggaca
    31081 atcacgtgga ccaatggctc acggttctcg ttaccaccgt cgtccaggtt ctatgggacc
    31141 tgttgcacct aaccgcgtat tcaaaggtaa aaaccttgca ggacgtatgg gtggtgaccg
    31201 cgtaacaatt caaaatcttg aagttgtaca agttgttcca gaaaagaacg ttatccttat
    31261 caaaggtaac gtaccaggtg ctaagaaatc tcttatcact atcaaatcag cagttaaagc
    31321 tggtaaataa taaagaaagg ggaaatcagt cacaatggca aacgtaacat tatttgacca
    31381 aactggtaaa gaagctggcc aagttgttct tagcgatgca gtatttggta tcgaaccaaa
    31441 tgaatcagtt gtgtttgatg taatcatcag ccaacgcgca agccttcgtc aaggaacaca
    31501 cgctgttaaa aaccgctctg cagtatcagg tggtggacgc aaaccatggc gtcaaaaagg
    31561 aactggacgt gctcgtcaag gttctatccg ctcaccacaa tggcgtggtg gtggtgttgt
    31621 cttcggacca actccacgtt catacggcta caaacttcca caaaaagttc gtcgcctagc
    31681 tcttaaatca gtttactctg aaaaagttgc tgaaaacaaa ttcgtagctg tagacgctct
    31741 ttcatttaca gctccaaaaa ctgctgaatt tgcaaaagtt cttgcagcat tgagcatcga
    31801 ttctaaagtt cttgttatcc ttgaagaagg aaatgaattc gcagctcttt cagctcgtaa
    31861 ccttccaaac gtgaaagttg caactgctac aactgcaagt gttcttgaca tcgcaaatag
    31921 cgacaaactt cttgtcacac aagcagctat ctctaaaatc gaggaggttc ttgcataatg
    31981 aatttgtatg atgttatcaa aaaacctgtc atcactgaaa gctcaatggc tcaacttgaa
    32041 gcagggaaat atgtatttga agttgacact cgtgcacaca aacttttgat caagcaagct
    32101 gttgaagctg ctttcgaagg tgttaaagtt gccaatgtta acacaatcaa cgtaaaacca
    32161 aaagctaaac gtgttggacg ttacactggt tttactaaca aaactaaaaa agctatcatc
    32221 acacttacag ctgattctaa agcaatcgag ttgtttgctg ctgaagctga ataatctaag
    32281 gaggaaatat cgtgggaatt cgtgtttata aaccaacaac aaacggtcgc cgtaatatga
    32341 cttctttgga tttcgctgaa atcacaacaa gcactcctga aaaatcattg cttgttgcat
    32401 tgaagagcaa ggctggtcgt aacaacaacg gtcgtatcac agttcgtcac caaggtggtg
    32461 gacacaaacg tttctaccgt ttggttgact tcaaacgtaa taaagacaac gttgaagcag
    32521 ttgttaaaac aatcgagtac gatccaaacc gttctgcaaa catcgctctt gtacactaca
    32581 ctgacggtgt gaaagcatac atcatcgctc caaaaggtct tgaagtaggt caacgtatcg
    32641 tttcaggtcc agaagcagat atcaaagtcg gaaacgctct tccacttgct aacatcccag
    32701 ttggtacttt gattcacaac atcgagttga aaccaggtcg tggtggtgaa ttggtacgtg
    32761 ctgctggtgc atctgctcaa gtattgggtt ctgaaggtaa atacgttctt gttcgtcttc
    32821 aatcaggtga agttcgtatg attcttggaa cttgccgtgc tacagttggt gttgtcggaa
    32881 acgaacaaca tggacttgta aaccttggta aagcaggacg tagccgttgg aaaggtatcc
    32941 gcccaacagt tcgtggttct gtaatgaacc ctaacgatca cccacacggt ggtggtgaag
    33001 gtaaggcacc agttggtcgt aaagcaccat ctactccatg gggcaaacct gctcttggtc
    33061 ttaaaactcg taacaagaaa gcgaaatctg acaaacttat cgttcgtcgt cgcaacgaga
    33121 aataatatta aactagtcgc ttaagcaact agtaaatccg ccagctcggt agcgctccat
    33181 aggagtgcaa gccgctgtgg tacaacattt aaaggagaaa atataaaaat gggacgcagt
    33241 cttaaaaaag gacctttcgt cgatgagcat ttgatgaaaa aagttgaagc tcaagctaac
    33301 gacgaaaaga aaaaagttat taaaacttgg tcacgtcgat caacgatctt cccaagtttc
    33361 attggttaca ctattgcagt ttatgacgga cgtaaacacg tacctgttta catccaagaa
    33421 gacatggtag gccacaaact tggtgaattt gcaccaactc gtacttacaa aggtcacgct
    33481 gcagacgaca agaaaacacg tagaaaataa ggagaacata aatggcagaa attacttcag
    33541 ctaaagcaat ggctcgtaca gtacgtgttt cacctcgtaa atcacgtctt gttcttgata
    33601 acatccgtgg taaaagcgta gccgatgcaa tcgcaatctt gacattcact ccaaacaaag
    33661 ctgctgaaat catcttgaaa gttttgaact cagctgtagc taacgctgaa aacaactttg
    33721 gtttggataa agctaacttg gtagtatctg aagcattcgc aaacgaagga ccaactatga
    33781 aacgtttccg tccacgtgcg aaaggttcag cttcaccaat caacaaacgt acagctcaca
    33841 tcactgtagc tgttgcagaa aaataaggag gtaaaatcgt gggtcaaaaa gtacatccaa
    33901 ttggtatgcg tgtcggcatc atccgtgatt gggatgccaa atggtatgct gaaaaagaat
    33961 acgcggatta ccttcatgaa gatcttgcaa tccgtaaatt cgttcaaaaa gaacttgctg
    34021 acgcagcagt ttcaactatt gaaatcgaac gcgcagtaaa caaagttaac gtttcacttc
    34081 acactgctaa accaggtatg gttatcggta aaggtggtgc taacgttgat gcactccgtg
    34141 caaaacttaa caaattgact ggaaaacaag tacacatcaa catcatcgaa atcaaacaac
    34201 ctgatttgga tgctcacctt gtaggtgaag gaattgctcg tcaattggag caacgtgttg
    34261 ctttccgtcg tgcacaaaaa caagcaatcc aacgtgcaat gcgtgctgga gctaaaggaa
    34321 tcaaaactca agtatcaggt cgtttgaacg gtgcagatat cgcccgtgct gaaggatact
    34381 ctgaaggaac tgttccgctt cacacacttc gtgcagatat cgattacgct tgggaagaag
    34441 cagatactac atatggtaaa cttggtgtta aagtatggat ctaccgtggt gaagttcttc
    34501 cagctcgtaa aaacactaaa ggaggtaaat aaccaatgtt agtacctaaa cgtgttaaac
    34561 accgtcgtga attccgtgga aaaatgcgcg gtgaagcaaa aggtggaaaa gaagtagcat
    34621 tcggtgaata cggtcttcaa gctacaacta gccactggat cactaaccgc caaatcgaag
    34681 ctgctcgtat cgccatgact cgttacatga aacgtggtgg taaagtttgg attaaaatct
    34741 tcccacacaa atcatacact gctaaagcta tcggtgtgcg tatgggatct ggtaaagggg
    34801 cacctgaagg ttgggtagca ccagttaaac gtggtaaagt gatgttcgaa atcgctggtg
    34861 tatctgaaga gattgcacgt gaagcgcttc gacttgctag ccacaaattg ccagttaaat
    34921 gtaaattcgt aaaacgtgaa gcagaataag gagaaggcat gaaacttaat gaagtaaaag
    34981 aatttgttaa agaacttcgt ggtctttctc aagaagaact cgcgaagcgc gaaaacgaat
    35041 tgaaaaaaga attgtttgaa cttcgtttcc aagctgctac tggtcaattg gaacaaacag
    35101 ctcgcttgaa agaagttaaa aaacaaatcg ctcgtatcaa aacagttcaa tctgaagcga
    35161 aataatagac tagggaagga gaaatttcaa tggaacgcaa taatcgtaaa gttcttgttg
    35221 gacgtgttgt atctgacaaa atggacaaga caatcacagt tgtagttgaa acaaaacgta
    35281 accacccagt ctatggtaaa cgtattaact actctaaaaa atacaaagct catgatgaaa
    35341 acaatgttgc caaagaaggc gatatcgtac gtatcatgga aactcgcccg ctttcagcta
    35401 caaaacgttt ccgtcttgta gaagttgttg aagaagcggt catcatctaa tcaaacctga
    35461 aaggagaaaa ctgaaatgat tcaaacagaa actcgtttga aagtcgcaga caacagcggt
    35521 gctcgcgaaa tcttgactat caaagttctt ggtggttcag gacgtaaatt tgcaaacatc
    35581 ggtgatgtta tcgtggcatc tgtaaaacaa gctactcctg gtggtgcggt taaaaaaggt
    35641 gacgttgtta aagcagttat cgttcgtact aaatcaggtg ctcgtcgtgc tgatggttca
    35701 tacatcaaat ttgacgaaaa cgcagcagtt atcatccgtg aagacaaaac tcctcgcgga
    35761 acacgtatct ttggcccagt tgcacgtgaa ttgcgtgaag gtggcttcat gaagatcgtg
    35821 tcacttgctc cagaagtact ttaattttta ggaacaaact agtcccctag cttcaagcta
    35881 gggtgccctt atgggcgtaa gaaaaatcaa ggagaaacct aatgtttgta aaaaaaggcg
    35941 acaaagttcg cgtaatcgct ggtaaagata agggaacaga agctgttgtc cttactgccc
    36001 ttccaaaagt aaacaaagtt atcgttgaag gtgttaacat tgttaagaaa caccaacgtc
    36061 caactaacga gcttcctcaa ggtggtatca tcgagaaaga agcagctatc cacgtatcaa
    36121 atgttcaagt tttggataaa aatggtgtag ctggtcgtgt tggttacaaa tttgtagacg
    36181 gtaaaaaagt tcgctacaac aaaaaatcag gcgaagtgct tgattaatca cgaaggaaag
    36241 gagaagtata atggcaaatc gtttaaaaga aaaatatctt aatgaagtag ttcctgcttt
    36301 gacagaacaa ttcaactact catcagtgat ggctgtgcct aaagtagata agattgtttt
    36361 gaacatgggt gttggtgaag ctgtatcaaa cgctaaaagc cttgaaaaag ctgctgaaga
    36421 attggcactt atctcaggtc aaaaaccact tatcactaaa gctaaaaaat caatcgccgg
    36481 cttccgtctt cgtgaaggtg ttgcgatcgg tgcaaaagtt acccttcgtg gtgaacgtat
    36541 gtacgaattc ttggataaat tggtatcagt ttcacttcca cgtgtacgtg acttccacgg
    36601 tgtcccaaca aaatcatttg atggacgcgg gaactacaca cttggtgtga aagaacaatt
    36661 aatcttccca gaaatcaact tcgatgacgt tgacaaaact cgtggtcttg acatcgttat
    36721 cgtaacaact gctaacactg acgaagagtc acgtgcattg cttacaggcc ttggaatgcc
    36781 ttttgcaaaa taatatagga ggtaaatcta atggctaaaa aatcaatggt agctagagag
    36841 gctaaacgcc aaaaaattgt tgaccgttat gctgaaaaac gtgctgcatt aaaggcggca
    36901 ggggactacg aaggtttatc taaattacct cgcaacgcct caccgactcg tttacataat
    36961 cgttgtaggg ttacggggcg cccacattca gtttaccgca aatttggtct gagtcgtatc
    37021 gcttttcgcg aacttgcgca taaaggtcaa attcctggtg taacaaaagc atcttggtaa
    37081 tttaagatat caagagtgtc aaaactccaa gtaaaaatag gaaacttgac gaagaaacta
    37141 aagtttctag gaaagtttat ctttttcaca cagagtttag cccgggttca attgggcttg
    37201 ccaatttgaa cacgagctac agctttggca aaaaagacca atttgctttg gagcattgct
    37261 tctgcattaa attgtctatt tttgctcgtg ctgttacgct ctttgtatca tgtattaact
    37321 agcaagtgca acttgcaaac tactagtaag aggagaaaaa caaaatggtt atgactgacc
    37381 caatcgcaga cttcctaact cgtattcgta atgctaacca agctaaacac gaagtacttg
    37441 aagtacctgc atcaaacatc aaaaaaggga ttgctgaaat ccttaaacgc gaaggttttg
    37501 taaaaaacgt tgaaatcatt gaagatgaca aacaaggcgt catccgtgta tttcttaaat
    37561 acggaccaaa tggtgagaaa gttatcacta acttgaaacg tgtttctaaa ccaggacttc
    37621 gtgtctacaa aaaacgtgaa gaccttccaa aagttcttaa cggacttgga attgccatcc
    37681 tttcaacttc tgaaggtttg cttactgata aagaagcacg ccaaaagaat gttggtggtg
    37741 aggttatcgc ttacgtttgg taaaatcaag atacaaagct cgtaaagaac aaagcaaaat
    37801 taggaagttg gagaagtttg tttacaaaca agccaactta tctattttgc acagttctta
    37861 gagcgtgttc agttcagctc ttgaactaaa taagtatctg aaccccgtga aaactggccg
    37921 ttctggcctg acaatttaac aggagaaaat aaacatgtca cgtattggta ataaagttat
    37981 cgtgttgcct gctggtgttg aactcgctaa caatgacaac gttgtaactg taaaaggacc
    38041 taaaggagaa cttactcgtg agttctcaaa agatattgaa atccgtgtgg aaggtactga
    38101 aataactctt caccgtccaa acgattcaaa agaaatgaaa actatccacg gaactactcg
    38161 tgcccttttg aacaacatgg ttgttggtgt atcagaagga ttcaagaaag aacttgaaat
    38221 gcgtggggtt ggttaccgtg cacagcttca aggatctaaa cttgttttgg ctgttggtaa
    38281 atctcatcca gacgaagttg aagctccaga aggaattact tttgaacttc caaacccaac
    38341 aacaatcgtt gttagcggaa tttcaaaaga agtagttggt caaacagctg cttacgtacg
    38401 tagccttcgt tcaccagaac catataaagg taaaggtatc cgttacgttg gtgaattcgt
    38461 tcgccgtaaa gaaggtaaaa caggtaaata atgttgagtg gttgatcatc aaccaccaac
    38521 ctattttcca actttgtgca tagcacacga tttaaaacta aagaggtgaa aactgtgatt
    38581 tcaaaaccag ataaaaacaa actccgccaa aaacgccacc gtcgcgttcg cggaaaactc
    38641 tctggaactg ctgatcgccc acgtttgaac gtattccgtt ctaatacagg catctacgct
    38701 caagtgattg atgacgtagc gggtgtaacg ctcgcaagtg cttcaactct tgataaagaa
    38761 gtttcaaaag gaactaaaac tgaacaagcc gttgctgtcg gtaaactcgt tgcagaacgt
    38821 gcaaacgcta aaggtatttc agaagtggtg ttcgaccgcg gtggatatct atatcacgga
    38881 cgtgtgaaag ctttggctga tgcagctcgt gaaaacggat tgaaattcta ataggaggac
    38941 actagaaaat ggcatttaaa gacaatgcag ttgaattaga agaacgcgta gttgctgtca
    39001 accgtgttac aaaagttgtt aaaggtggac gtcgtcttcg tttcgcagct cttgttgttg
    39061 ttggtgacca caatggtcgc gtaggatttg gtactggtaa agctcaagaa gttccagaag
    39121 caatccgtaa agcagtagat gatgctaaga aaaacttgat cgaagttcct atggttggaa
    39181 caacaatccc acacgaagtt ctttcagaat tcggtggagc taaagtattg ttgaaacctg
    39241 ctgtagaagg ttctggagtt gccgctggtg gtgcagttcg tgccgttgtg gaattggcag
    39301 gtgtggcaga tattacatct aaatcacttg gttctaacac tccaatcaac attgttcgtg
    39361 caactgttga aggtttgaaa caattgaaac gcgctgaaga aattgctgcc cttcgtggta
    39421 tttcagtttc tgatttggca taagaaaggg gataaaatgg ctcaaattaa aattactttg
    39481 actaagtctc caatcggacg cattccatca caacgtaaaa ctgttgtagc acttggactt
    39541 ggcaaattga acagctctgt tattaaagaa gataacgctg ctatccgtgg tatgatcaca
    39601 gcagtatctc acttagtaac agttgaagaa gtaaactaat gaagttttag gggatgtgca
    39661 ctgtaccatc ccctaaaact agatatagtc atctatgatg acatcgtata ggcgagttga
    39721 tgggggagac aaccttttct cccttatcgg cgctagcatt ttacaaaaga ggagaaaata
    39781 aaaatgaaac ttcatgaatt gaaacctgca gaaggttctc gtaaagtacg taaccgcgtt
    39841 ggtcgtggta cttcatcagg taacggtaaa acatctggtc gtggtcaaaa aggtcaaaaa
    39901 gctcgtagcg gtggcggagt tcgccttggt tttgaaggtg gacaaactcc attgttccgt
    39961 cgtcttccaa aacgtggatt cactaacatc aacgctaaag aatacgcaat tgtgaacctt
    40021 gaccaattga acgtctttga agatggtgct gaagtaactc cagttgttct tatcgaagca
    40081 ggaattgtta aagctgaaaa gtcaggtatt aaaattcttg gtaacggtga gttgactaag
    40141 aaattgactg tgaaagcagc taaattctct aaatcagctg aagaagctat cactgctaaa
    40201 ggtggttcag tagaagtcat ctaagagagg tgacctatgt tttttaaatt attaagagaa
    40261 gctcttaaag tcaagcaggt tcgatcaaaa attttattta caatttttat cgttttggtc
    40321 tttcgtatcg gaactagcat tacagttcct ggtgtgaatg ccaatagctt gaatgcttta
    40381 agtggattat ccttcttaaa catgttgagc ttggtgtcgg ggaatgccct aaaaaacttt
    40441 tcgatttttg ccctaggagt tagtccctat atcactgctt ctattgttgt ccaactcttg
    40501 caaatggata ttttacccaa gtttgtagag tggggtaaac aaggggaagt aggtcgaaga
    40561 aaattgaatc aagctactcg ttatattgct ctagttctcg cttttgtgca atctatcggg
    40621 attacagctg gttttaatac cttggctgga gctcaattga ttaaaactgc tttaactcca
    40681 caagtttttc tgacgattgg tatcatctta acagctggta gtatgattgt cacttggttg
    40741 ggtgagcaaa ttacagataa gggatacgga aacggtgttt ccatgattat ctttgccggg
    40801 attgtttcct caattccaga gatgattcag ggcatctatg tggactactt tgtgaacgtc
    40861 ccaagtagcc gtatcacttc atctatcatt ttcgtaatca ttttgattat tactgtattg
    40921 ttgattattt actttacaac ttatgttcaa caagcagaat acaaaattcc aatccaatat
    40981 actaaggttg cacaaggtgc tccatctagc tcttaccttc cgttaaaggt aaatcctgct
    41041 ggagttatcc ctgttatctt tgccagttcg attactgcag cgcctgcggc tattcttcag
    41101 tttttgagtg ccacaggtca tgattgggct tgggtaaggg tagcacaaga gatgttggca
    41161 actacttctc caactggtat tgccatgtat gctttgttga ttattctctt tacattcttc
    41221 tatacgtttg tacagattaa tcctgaaaaa gcagcagaga gcctacaaaa gagtggtgcc
    41281 tatatccatg gagttcgtcc tggtaaaggt acagaagaat atatgtctaa acttcttcgt
    41341 cgtcttgcaa ctgttggttc cctcttcctt ggtgtgattt ccattttacc gattgcagct
    41401 aaagatgtat ttggtctttc tgatgttgtt gcctttggtg gaacaagtct cttgatcatt
    41461 atctctacag gtatcgaagg aatcaagcaa ttggaaggtt acctattgaa acgtaagtat
    41521 gttggtttca tggacagaac agaataaaag tatttactga atcagtaaat gctgagggag
    41581 tggaggttta aactctgaca tttgtaagag ttggatctcc cctcttctat tttgttttta
    41641 aataggggtg aaaagacttt ttgcttctat ttaaaaataa aataaggaga tcaaatcatg
    41701 aatcttttga ttatgggctt acctggtgca ggtaagggaa ctcaagcagc aaaaatcgta
    41761 gaacaattcc atgttgcaca tatctcaaca ggtgatatgt tccgcgctgc aatggcaaat
    41821 caaactgaaa tgggtgttct tgctaagtca tatattgaca agggtgaatt ggttcctgac
    41881 gaagttacaa atggaatcgt aaaagaacgc ctttcacaag atgatattaa agaaacagga
    41941 ttcttattgg atggttaccc acgtacaatt gaacaagctc atgccttgga caaaacattg
    42001 gctgaacttg gcattgaact agaaggtgtt atcaatattg aagtgaaccc tgacagcctc
    42061 ttggaacgtt tgagtggccg tatcatccac cgcgtaactg gagaaacttt ccacaaggtc
    42121 tttaacccac cagttgacta taaagaagaa gattactacc aacgtgaaga tgataagcct
    42181 gagacagtaa aacgtcgttt ggatgttaat attgctcaag gagaaccaat cattgctcac
    42241 taccgtgcca aaggtttggt tcatgacatc gaaggtaatc aagatatcaa tgatgtcttc
    42301 tcagatattg aaaaagtatt gacaaatttg aaataaagcg tttttcatac ttgcaaaaat
    42361 tcgctacaaa tgttatactg agatagtctg acttataatt gttgtctctg tgtctagagg
    42421 catcgaatcg aaatttatgg aggtgctttt gcgtggcaaa agacgatgtg attgaagttg
    42481 aaggcaaagt agttgataca atgccgaatg caatgtttac ggttgaactt gaaaatggac
    42541 atcagatttt agcaacagtt tctggtaaaa ttcgtaaaaa ctatattcgt attttagcgg
    42601 gagatcgtgt tactgtcgaa atgagtccat atgacttgac acgtggacgt atcacttacc
    42661 gctttaaata atcgaaaaac ttggagggat aagaaatgaa agtaagacca tcggtcaaac
    42721 caatttgcga atactgtaaa gttattcgtc gtaatggtcg tgttatggta atttgcccag
    42781 caaatccaaa acacaaacaa cgtcaaggat aagatagaaa ggagaaaaca tggctcgtat
    42841 tgctggagtt gatattccaa atgacaaacg cgtagtaatc tcattgactt atgtttatgg
    42901 tatcggactt gcaacatcta agaaaatttt ggctgctgct ggaatctcag aagatgttcg
    42961 tgtacgtgat cttacatcag atcaagaaga tgctatccgt cgtgaagtgg atgcaatcaa
    43021 agttgaaggt gaccttcgtc gtgaagtaaa cttgaacatc aaacgtttga tggaaatcgg
    43081 ttcataccgt ggtatccgtc accgtcgtgg acttcctgtc cgtggacaaa acactaaaaa
    43141 caacgctcgc actcgtaaag gtaaagctgt tgcgattgct ggtaagaaaa aataatatag
    43201 gaggtaaaag tcttggctaa accaacacgt aaacgtcgtg tgaaaaagaa tatcgaatct
    43261 ggtattgctc atattcacgc tacatttaat aacactattg ttatgattac tgatgtgcat
    43321 ggtaatgcaa ttgcttggtc atcagctggt gctcttggtt tcaaaggttc tcgtaaatct
    43381 acaccattcg ctgctcaaat ggcttctgaa gctgctgcta aatctgcaca agaacacggt
    43441 cttaaatcag ttgaagttac tgtaaaaggt ccaggttctg gtcgtgagtc agctattcgt
    43501 gcgcttgctg ccgctggtct tgaagtaaca gcaattcgtg atgtgactcc agtgccacac
    43561 aatggtgctc gtcctccaaa acgtcgccgt gtataatcat cgcattacac tgcttttcgt
    43621 ttaagaggga gtaactaaat gatcgagttt gaaaaaccaa atataacaaa aattgatgaa
    43681 aataaagatt atggcaagtt tgtaatcgaa ccacttgaac gtggctacgg tacaactctt
    43741 ggtaactctc ttcgtcgtgt acttctagct tctctaccag gagcagctgt gacatctatc
    43801 aacattgatg gtgtgttaca tgagtttgac acagttccag gtgttcgtga agacgtgatg
    43861 caaatcattc tgaacattaa aggaattgca gtgaaatcgt acgttgaaga cgaaaaaatc
    43921 atcgaactgg atgttgaagg tcctgctgaa gtaacagctg gtgacatttt gacagatagc
    43981 gatattgaaa ttgtaaatcc agatcattat ctctttacaa tcggtgaagg ttcttctcta
    44041 aaagcgacta tgactgttaa cagtggtcgt ggatatgtac ctgctgatga aaataaaaag
    44101 gataatgcac cagttggaac acttgctgta gattctattt atacaccagt tacaaaagtc
    44161 aactatcaag tggaacctgc tcgtgtaggt agcaatgatg gtttcgacaa attaaccctt
    44221 gaaatcttga caaatggaac aattattcca gaagatgctt tagggctttc agcacgtatt
    44281 ttgacagaac atcttgattt gtttacaaat cttactgaga ttgctaagtc aactgaagtg
    44341 atgaaagaag ctgatactga atctgacgac cgtattttag atcgtacgat tgaggaactg
    44401 gacttgtctg tgcgttcata caactgttta aaacgtgccg gtatcaatac tgtgcatgat
    44461 ttgacagaaa aatctgaagc agagatgatg aaagtacgaa atcttggacg caagagtttg
    44521 gaagaagtga aactcaaact cattgatttg ggtcttggat taaaagataa ataaaggagg
    44581 aatacatggc ttaccgtaaa ctaggacgca ctagctcaca acgtaaagca atgcttcgcg
    44641 atttgacaac tgaccttttg atcaacgaat caatcgtgac aactgaagct cgtgctaaag
    44701 aaatccgtaa aactgttgaa aaaatgatta ctctaggtaa acgtggtgat ttgcatgcac
    44761 gtcgtcaagc agctgctttc gtacgtaatg aaatcgcatc tgaaaactat gatgaagcaa
    44821 ctgataagta cacttctact acagcacttc aaaaattgtt ctcagaaatc gcacctcgtt
    44881 atgctgaacg taacggtgga tacactcgta tccttaaaac tgaaccacgt cgtggtgatg
    44941 cagcgccaat ggcgatcatc gaattagtat aaaatcatca attttgttga gtgttatgat
    45001 gatggagtct tgtgctctta gtctagctct ggtctaccgc taggatttcg gtcctagcgg
    45061 gaacactcat cataagttgg gatagtagac gcttgtttac gaaattgttt ttttcttaag
    45121 aacaacttcg taagcaggcg tttttgagta ttttcgttag aattatgcta tactatttga
    45181 aaagaatcct gtttaatgtt aaggtttctt attttaagaa gaattggagt ttacttatga
    45241 aagccattat aactgttgtt ggtaaagata aatctggaat tgttgcaggt gtttctggta
    45301 aaattgcaga attaggattg aatattgacg atatctctca aactgtattg gatgaatatt
    45361 ttacgatgat ggctgttgta tctagtgatg aaaagcaaga ttttacctat cttcgtaatg
    45421 aatttgaagc ttttgggcaa actttgaatg taaaaatcaa tattcagagt gcagcgattt
    45481 tcgaagctat gtataatatc taggaggtca tcatggatat tagacaagtt actgaaacca
    45541 tcgccatgat tgaggagcaa aacttcgata ttagaaccat taccatgggg atttctcttt
    45601 tggactgtat cgatccaaat atcaatcgtg ctgcggagaa aatctatcaa aaaattacga
    45661 caaaggcggc taatttagta gctgttggtg atgaaattgc ggctgagttg ggaattccta
    45721 tcgttaataa gcgtgtatcg gtgacaccta tttctctgat tggggcagcg acagatgcga
    45781 cggactacgt ggttctggca aaagcgcttg ataaggctgc gaaagagatt ggtgtggact
    45841 ttattggtgg tttttctgcc ttagtacaaa aaggttatca aaagggagat gagattctca
    45901 tcaattccat tcctcgcgct ttggctgaga cggataaggt ctgctcgtca gtcaatatcg
    45961 gctcaaccaa gtctggtatt aatatgacgg ctgtggcaga tatgggacga attatcaagg
    46021 aaacagcaaa tctttcagat atgggagtgg ccaagttggt tgtattcgct aatgctgttg
    46081 aggacaatcc atttatggcg ggtgcctttc atggtgttgg ggaagcagat gttatcatca
    46141 atgtcggagt ttctggtcct ggtgtggtga aacgtgcttt ggaaaaagtt cgtggacaga
    46201 gctttgatgt agtagccgaa acagttaaga aaactgcctt taaaatcact cgtatcggtc
    46261 aattggttgg tcaaatggcc agtgagagac tgggtgtgga gtttggtatt gtggacttga
    46321 gtttggcacc aacccctgcg gttggagact ctgtggcacg tgtccttgag gaaatggggc
    46381 tagaaacagt tggcacgcat ggaacgacag ctgccttggc cctcttgaac gaccaagtta
    46441 aaaagggtgg agtgatggcc tgtaaccagg tcggtggtct atctggtgcc tttatccctg
    46501 tttctgagga tgaaggaatg attgctgcag tgcaaaatgg ctctcttaat ttagaaaaac
    46561 tagaagctat gacggctatc tgttctgttg gattggatat gattgccatc ccagaagata
    46621 cgcctgctga aactattgcg gctatgattg cggatgaagc agcaatcggt gttatcaaca
    46681 tgaaaacaac agctgttcgt atcattccca aaggaagaga aggcgatatg attgagtttg
    46741 gtggtctatt aggaactgca cctgttatga aggttaatgg ggcttcgtct gtcgacttca
    46801 tctctcgcgg tggacaaatc ccagcaccaa ttcatagttt taaaaattaa gaaaatagga
    46861 gaaattttaa gttctattta agattagacg tgtatactat aatcattaaa taaagacctc
    46921 ctaatattat ttgaaacaga taacactgaa ttagtttgaa tttgattttc atctaatatc
    46981 tttatttaat gaactcctaa actttttcat aataatctcc ttcaaaagtc gcctgtatgg
    47041 gtggctttta ttttatcatt catgatataa tagaagcaaa cggaggacgg aaaatggtaa
    47101 aagtacgatt gtatttggta cgtcatggca agaccatgtt taacacgatt ggtcgcgcgc
    47161 aaggttggag cgatactccc ttaactgctg aaggtgaacg agggattcaa gagttaggaa
    47221 ttggtttgcg agaatctgat ctacagtttg agcgcgctta ttcgagtgat tctggtcgta
    47281 ccattcagac catgggaatt atccttgaag aacttggctt gcaggggaaa atcccttatc
    47341 gcatggacaa gcgtatcaga gaatggtgtt tcggtagttt tgatggagcc tatgatggcg
    47401 atcttttcat gggcattatt cctcgtatct ttaatgtgga ccacgttcac caattgtctt
    47461 atgctgaact ggctgagggc ttggtagagg tcgatacagc tggttgggct gaaggctggg
    47521 aaaaactcag tggccgaatc aaggaaggct ttgaaatgat tgcaaaagaa atggaagatc
    47581 aaggtggagg taacgccctt gttgtcagcc atggaatgac tattggaacc attgtttatc
    47641 tgattaatgg catgcatccg catggtctag ataatggtag cgtgacgatt cttgaatatg
    47701 aggacggtca gtttagggtt gaagttgtcg gtgaccgtag ttaccgagag ctaggacgtg
    47761 agaagatgga agaaggctct atttaatcag tctagacttg cttgccatga gctagggatt
    47821 tgataagaat atcaagataa gaaaaaacag ccgagggtac tcctttcggc tgtttttgat
    47881 gtggaaaact aaagtgtaat gctattgctt ttagagattt tcataaacaa gagcaaggaa
    47941 cctactgtta gaacagtcag gatagttgac aaggctgcgg ctacaccgta atttcctctg
    48001 agaacctctg tataaatagc tacagtcatt gttcttgttt tgacattgta gaggaggata
    48061 gaagtagaga gttttgaaat cattatgacc taagataaga tggctccaga aatgatacca
    48121 gatagcatca ttggagttgt aatcttagca aaggtattga gacgactact tcctaagctt
    48181 tcagcagctt cttcaatact tggtgctatt tgttgtaagc tagcaacaga tgagcgaata
    48241 gtataaggta atcttctgac agatagagac ataatcaaga tgaaagcagt ccctgtaatc
    48301 ataagaaatc cacttccaaa tagaccagta ttgaaggaag aaatgaaggc aatccctaga
    48361 acggttcctg gtacaatata aggtaccata ctgaggctgt caattaagtt tgtaaacaaa
    48421 ttccgttttc taacggctag gtaggagata aatgtcgcaa atagaacaac tagaactaag
    48481 gcaatcaaag ggatacgaat ggtattgaaa atagcagatc ccatacgatt gaaagctacc
    48541 ttgtaactgt ttggagaata acctttaaca gataccatac ctgatgtttt taggaaagag
    48601 gtataaatta agtagatttg aggtaaaaca gagataaaga taattccgta gactgttgca
    48661 taaatggcag ctatttttcc ttttgtagtt tttttaggct caattggatg gagcagattc
    48721 atgctgaaac tgtagcggtt tgcaatgtgt ttttggataa ggaaaattgc caagacaatg
    48781 ataatcgcca taattgcaaa agcagaattt cctccaacct cgctaataaa ttgggtataa
    48841 atcaggacag ggaaggtccg atatccttcg ccaatcaaca taggcgttcc aaagtctgag
    48901 aatgctctca taaatacaag caaggagctg ctagtaaggt tggaactagg agaggtaaaa
    48961 caaccgttac gataggttta aatccgaagg accccatgct ttcagctgct tcaagtagag
    49021 aattgtcaat actgttcatt gttccagcaa catatagaaa taccagtggg aatagttgca
    49081 gtgtaaagac aagtacaatt cctttgaatc cataaatatc gatagctgga agatgaaggg
    49141 catttgtcaa aaatttagtg atgaccccat ttcgtcctag caagagaacc caggagtagg
    49201 ctcctacgaa aggagctgac atggaagcaa tgataatcaa tatttgtaga aatttcttcc
    49261 ccttgaagtc atacatagag aagagataag ctaatagggt tcctacaact aaggaagtga
    49321 cagtagcggt aatggaaacc ttgaaactgt tgactagtgt ctcagagtag tagactttac
    49381 taaagaaagt gacaaaatta gctagtgaga attgtccttc atgtataagt gcttgcttga
    49441 gcacggtaac gataggataa acgagaaaga caagataggt aagaaagatg aagaaagagg
    49501 aggttgtcca aatatttagt tttttacatt ccatggttga ctccttttat caggttttgg
    49561 gaaccatctg cagaaaagat gtttaatttt tgcgtattga ttcgtagacg aatacgattg
    49621 cctttttgta gttcttcttc aaaagttgat tcttcactaa cttgaatttt tgaggcaaaa
    49681 cctgtctcaa tgaaatactc cgtatttagt ccaagataga cgctatcgct aatagttcct
    49741 tcaatatctc cagattcatc tttgataaac tcttcgggac gaatgcttac atgaatagct
    49801 tgctcctcaa cctgatcaag agctggcatt cgaagggcat agccatctga aaagacgata
    49861 taagcgccgt cgctccgttt ttcaagatta gtagggataa tatttgtgcg tccgataaag
    49921 gttgccacaa actcattagc tggtttatga tagagttctt ttggtcggcc gatttgttgg
    49981 atcaccccat ctttcataac agcaatttgg tctgaaatag ccatggcttc ttcttggtcg
    50041 tgggttacat aaacagttgt aattcccact tcgtgttgga tttctcggat ggcttgacgc
    50101 atatccaagc gaagtttggc ctccagatta ctaagtggct cgtccatgag gagaacactt
    50161 ggattaaccg ctaaggcgca tgccaaggtg acacgttgtt gttgtccacc actgagttta
    50221 tcgggctttc aatccgcata ttgagcaatt tgcatgagtt caagatactt gttggtctgt
    50281 tgaatcaatt cttcttttgg aaccttcttt tgcataagac caaaagcaac gttgtctcgg
    50341 acagtcaaat gtgggaaaat agcgtagttt tggaaaacca gcccgatatt gcgtttgctg
    50401 ggttccatat tattgatttt tgtatcatcg aagtaaaatt ctccatcttt gatactgttg
    50461 aaacctgcaa tcatacgaag aagggtcgtt ttcccacatc ctgaagctcc aagaagggta
    50521 aagagacttc cttttggaat tgtaatgttc aaattctcaa taacagggac atcgtggtag
    50581 atttttttgg cgttaataat ttcgatctca ctcatagtga acctctttta ctgtttagat
    50641 tggatatctg taaagacttc gttgtatttc ttaacgatat ctgatttatt cttgatgaca
    50701 taatcataat cttcagtgag tgttttgatt ttgtcaattg gtttcatgtt ttcgcttgtt
    50761 ttagcatttt tacgaacagg acggttagta gtggttgtac caagtgtatc ttgtacttct
    50821 tgagagataa taaaatcgat aaatttcttg gcattttcca tatttttagc ttttttaacg
    50881 atagcagcac tagcaggtag gaagacggtt ccttcttttg gatagactac tttaatgtta
    50941 gctccgtcat ttaagagttt aactgctgga tcctcataag agagaccaac agccatttct
    51001 ccatcaacga ctgctttata gacaccagat gaacttgaac cgattttata gtgtattgaa
    51061 actagaatag tacacctcta cttctaaaac attgttagaa atcgatttga ctgtcctgat
    51121 taatttgtcc tgttcttatt tcattttact ataccatcaa taagtgtgaa aagatctttt
    51181 acataagacc aagccttatc atctttgtaa ccaccttgag cttgtagcat atttgttaat
    51241 tgagcaaagg cgctagaaga gtttgctggg tcagcagttg cgatttttcc ttttagttca
    51301 ggtttgagaa ggtcgctata tccttcgatg ttcatgcctt tagttaaatc agggtggacg
    51361 attaaaacac taccatctag tgtataagga gtagagaagc cagttgtgtt ttgatattct
    51421 ttgataacat tatcattttc ttttgaaata tagttttcaa agagttctcc gtgggtagca
    51481 tattgtgtat aagaaccacc aaagataaca tcagctacag gaacttcttt ttctgactct
    51541 agttttttga aaagttctcc agtaccagct tgaatcagtt ctattttgat accatatttt
    51601 tcttcaaagg caggaatagt tgctccaatt aagccttctg agtttggtga ataaacgact
    51661 agcgaaccac cgtctccttt atcagatgaa ctgtcatcgg cagattcatt agaagaacaa
    51721 gcagcataat acatccattt ctttttcatg atggatatct ccgttgtgtt atttaagttt
    51781 attttaaaac aatgtaagcg tttttaaaac ctacaattct attctatagt gtattgaatc
    51841 tataacagta cactttgact gctaaaatat ttctataaat taatttgact ttcctgatag
    51901 agatgttcac atcttatttc aattcactat attagagtaa aattctctac aaaaagaaga
    51961 atagcctatt tgactattct tccgagtgat ttcaatttct ttggggaaat atggagatac
    52021 tttttaaata ctttggcaaa atatttatag tctgaaaatc caacttggtc agagatagcg
    52081 cttaaagggg agttgggaga ttcagccatc ttatctatag cttgtttcag gcgatattgt
    52141 aagatatatt catttagagt catggggaag tcttctttga taaccttata tagatgcttg
    52201 ttatctgcaa atagctagag catttcaagg aaataaacta gaaataacaa ttaatatggt
    52261 ggttcagaaa taccttttga taatcaataa gatcttcagg atgaagtgcg ttgattccat
    52321 ccatttcata tttccgattt aataaacgat atttgttttc accaaattga tgaaaaggga
    52381 gtagttgaac ttggtcgata tttaatgagt taaatagagt agcgaatttt tctgcatcct
    52441 ctaaactatt gttaaaatta ggaataactg ggattcttaa aacgatagtt ttattttgag
    52501 aaaaagcata atgaatgttt ttaataatca tttgattaaa aaccccagtc acttttttat
    52561 gttttataga attataatgt tttaggtctg tgtagataaa atccacatat tgaattaaat
    52621 caataaattt ttcatgatca acaaaggcag tagtttcaat ggcagtgtgt atgtgatgtt
    52681 ctttagctga ttttaagatg gctttagcaa attcaaactg agcaaatatt tcacctcctg
    52741 ataaagttaa acctccaccg gattcttcgt aaaattcttt gtcttttaat acctctgtaa
    52801 taatttcttc tacagtcttt tcttcaccga ctaaggtgaa tttctctcgt tgagcatctt
    52861 tcattttttc aggtttcatt ctttgagatt caggattaga acaccatgga cagcgcagag
    52921 gacatccttt taaaaaaaca gttgtacgaa tacccgggcc gtcatgaatt gaaaagtgtt
    52981 gaatattaaa aataattcct ttagatattt ccataagagt tcctcctctt cataagtcta
    53041 tcctatcaca aaaacgaaag aaaaacaatt acgaacgaaa gtttaatttg ttgttttttg
    53101 ctttttattt tggtagaata aaacggaggt gtaaaatgaa aagattggaa caaattatta
    53161 aattagtatc agaatatgaa aagatcgacg ttaatacatt atcggaaaaa ttaaatgtat
    53221 cgaaagtaac gattagaaaa gatttagata aattagagtc aaaaggttta ttacacagag
    53281 agcatggata tgctgtatta aatagtggag atgacttaaa tgtacgtttg tcaattaact
    53341 atgaaattaa gagaaaaatt gttcaggaag cagtaaaatt ggtgtcagat aatgaaacaa
    53401 taatgataga atctggatcg acctgtgctt tacttgctga ggaaatttgc aagcaaaaaa
    53461 gaaatgttac gattgtaaca aattcatttt ttatagcaaa ttttgtgaga gcttatgatt
    53521 catgtcgtat tattgttctt ggtggagagt ttcagaagga ttcacaggtt actgtgggac
    53581 ctttattaaa agaaatgata cagacttttc atgtgcatca agcttttgtt gggacagatg
    53641 gttacgataa agagatgggc tttacaggaa aagatttaat gcgcagtgag gtagttcaat
    53701 atatttcagc agcgtcggat aaagtcattg tactaactga ctcaagtaaa tttgataaaa
    53761 ggggtacagt aagaagattt gctttaagtc aagtctatga agtaataaca gacgaaaaac
    53821 tttctaaaca aaatatagct acattagaaa atgctgggat aatggttaag gtagtttcgt
    53881 aagaggttaa atgtatgaat caagatagga ataaactgct ctctaaaatt gcttatctgt
    53941 attatattga aaacttaaat cagtcacaaa tagcagcaaa attaggaatt tatagaacct
    54001 ctattagtag aatgttaaca gaagcaagga atgcaggaat tgttaaaatt gaaatagaga
    54061 attttgatac caatatgttt aagttggaaa attatgtaaa agaaaaatat ggtctggaaa
    54121 gtttagaaat tattccaaat gaatttgatg ataatccaac aattttatct gaaagaattt
    54181 ctcaagttgc agcaggtgtc cttaggaatc taattgatga taatatgaaa attggctttt
    54241 cttgggggaa aagtttaagt aatttagtag atttaattca cagtaaaagt gtccgaaatg
    54301 ttcacttcta tcctctagca ggtggtccta gtcacataca cgctaaatac catgtgaata
    54361 cactgattta tgaaatgtct agaaaatttc atggagagtg tacatttatg aatgcaacga
    54421 ttgtgcaaga aaataaattg ttagcagatg gtattttgca atcaagatat tttgaaaatt
    54481 tgaaaaatag ttggaaagat ttagatatag ctgtagtcgg aattggtgat tttagcaata
    54541 aagggaaaca tcaatggtta gacatgctta cagaggatga ctttaaagaa ttgaccaaag
    54601 ttaaaactgt aggagaaatt tgttgtcgat tttttgattc aaaaggtaaa gaagtctatg
    54661 aaaacttaca ggaaagaacg atagccatct ctttggaaga tttaaaaaat attcctcaaa
    54721 gtttagctgt tgcttacggt gatacgaaag tatcttcgat tctttctgtc ttgcgtgcta
    54781 atttagtaaa tcatttgatt acagataaaa atacaatttt aaaagttttg gaagaagatg
    54841 gtgatttgac ttttagagag attctaggtg agtgaaaatg atagactgat cagtttatcg
    54901 ttttttcttt ttagtcgatt gcacatttgt gcttatataa acaaaaatag tttatctgtt
    54961 gtttttggat tgacaacttt attatgtagt tgtattctat agttacaaaa gaaaatttta
    55021 aaatttcaaa tgaaaaaagc tttttacata gtgaaatgag gaggaattta tggaaattat
    55081 tgttccagat caaattatca tgggtttaat tttatatgct ggcgatgcga aacaacatat
    55141 ttataaagca ttagattaca taaaaaatgg tacatgtgaa cggtgtgaag aagaaatcca
    55201 gttagctgat gccgccttat tagaagccca taatctacaa acaaaattct tggcacaaga
    55261 agcgtctggt acaaagacag aaattacagc tctctttgtt cattcacaag atcatctcat
    55321 gactagcatg acggagatta atttaatcaa agaaattatt agtttgagaa aagaacttca
    55381 taaaaaataa tactagagta ttatcattgt tattaacata gaggaggaaa acataatggt
    55441 gaagattggt ttgttttgtg cagcaggttt ttctactggt atgcttgtaa ataatatgaa
    55501 aattgcagcg caatctagtg gagttgaggc agaaatagag gcgttttctc agtctaaatt
    55561 agcggattat gcgccaaata tagatgttgc actattgggt ccacaagttg cttatacatt
    55621 agataaatca aaagaaattt gtgataagtg tgatgttccg atagctgtta ttccgatgat
    55681 ggactatggt atgttagatg ggaaaaaagt attagatttg gccctatctt tgattagtgg
    55741 gtaagaaaag gagatttatt atgtcaaaga tggatgttca gaaaatcatt gcaccgatga
    55801 tgaagtttgt gaatatgcgt ggcattatag ctctaaaaga tgggatgtta gcaattttgc
    55861 cattgacagt agttggcagt ttgttcttga ttatgggaca attgccgttc gaaggattaa
    55921 ataagagcat tgctagtgtt tttggagcta attggacaga gccgtttatg caagtatatt
    55981 caggaacttt tgctattatg ggtctaattt cttgtttttc aattgcctat tcttatgcta
    56041 agaatagcgg agtagaggct ttaccagctg gagttctatc tgtatctgca ttctttattt
    56101 tgctaagatc atcttatatc cctaaacaag gtgaggcgat tggggacgct attagtaaag
    56161 tttggtttgg aggccaagga attatcggtg ctatcattat aggtttggta gtaggaagta
    56221 tttatacctt ctttataaag agaaaaattg ttattaagat gccagaacaa gttccacaag
    56281 ctattgccaa acagtttgaa gcaatgattc cagcatttgt aattttctta tcttctatga
    56341 ttgtatatat tttagcgaag tcattgacta atggcggaac attcatagaa atgatttatt
    56401 ctgctattca agttccgttg caaggtttaa ctggatcttt gtatggtgct attggaattg
    56461 cattctttat atcatttttg tggtggtttg gtgttcatgg gcaatcggta gtaaatggag
    56521 tagtgacagc tctgctttta tctaatcttg atgctaataa agctatgtta gcctctggta
    56581 atctatcatt agaaaatggt gcacatattg ttactcaaca atttttagat tcatttttaa
    56641 ttctatcagg ttcagggatt acgtttggtc ttgtagttgc catgcttttt gcagcaaaat
    56701 caaaacaata ccaagcctta ggaaaagttg cagcttttcc agcaatattt aacgtaaatg
    56761 agccagttgt atttggattt ccgattgtca tgaatccagt tatgtttgta cctttcattc
    56821 ttgttcctgt acttgcagct gtgatagtat atggagctat tgcaacaggt ttcatgcaac
    56881 cattctcagg ggtaacattg ccttggagta caccagctat tctatcagga tttttggtgg
    56941 gtggatggca aggtgttgtg actcagttat tagtattggc gatgtctaca atggtttact
    57001 ttccattctt taagatacaa gatcgtttag cttgccaaaa tgaaatcaaa caatcttaga
    57061 ggtatttgtg tgttactgtt aaactcacac atttgtgcta aaaattagag agttaaaatt
    57121 tttctagtta aaagcttgaa aatttctata aaaatcggta ttatattttc gaaagaaata
    57181 aaaatatttt cgaaagaaag gtgcttacga tggtaaatac agaagtagca agaacaacaa
    57241 tcaagacaga atattttggc agccttactg aaaggatgaa caaatatcga gaagatgttt
    57301 taaataaaaa accttatatt gatgctgaga gagcagttct agcaacacgc gcctatgaac
    57361 gatacaagga acaacctaat gtcctaaaac gtgcatatat gctgaaagaa attttggaaa
    57421 atatgtctat ctatattgaa gaagaatcta tgattgcggg aaatcaagct tcttccaata
    57481 aagatgctcc tatttttccg gaatatacgc tagaatttgt tctcaatgag ttggatcttt
    57541 ttgaaaagcg tgatggagat gttttctata ttacagaaga aacaaaagaa caacttagaa
    57601 gtattgctcc gttttgggaa aataataatt tacgtgctag agctggtgcc ttattacctg
    57661 aagaagtgtc tgtttatatg gaaacaggat tcttcggtat ggaaggtaag atgaattctg
    57721 gagatgctca cttagcagtt aactatcaga aacttttgca atttggttta agaggttttg
    57781 aagagcgggc tcgtaaagcg aaggtagctc tagatttaac agatccagca agtattgata
    57841 aatatcattt ttacgactct atatttatcg taatcgatgc tattaaagta tatgcaaagc
    57901 gctttgttgc tcttgctaaa agtttagccg aaaatgcaaa tcctaaacgt aagaaagaat
    57961 tacttgagat tgcagatatt tgctctagag tcccatatga accggcaact acttttgcag
    58021 aagctattca atcagtttgg tttattcaat gtattttaca aattgaatct aatggccact
    58081 ctctttcata tggccgtttt gatcaatata tgtatccata tatgaaggct gatttagaaa
    58141 gtggtaaaga aacagaagat agcattgttg aacgtctgac aaatctttgg attaagacaa
    58201 ttacaattaa taaggttcgc agtcaatcac atacattttc ttcagcagga agtcctttat
    58261 atcaaaatgt tacaattggt ggacagactc gagataagaa ggatgctgtt aacccattat
    58321 cttatttggt attaaaatca gttgcacaaa cccatctacc gcaacctaat ctaactgtac
    58381 gttaccatgc aggtttagat gctcgtttca tgaatgagtg tattgaagtg atgaaacttg
    58441 gttttggtat gcctgcattt aataatgatg agattattat tccttctttt attgcaaaag
    58501 gagtattgga agatgatgct tatgattaca gtgccattgg atgtgttgaa acggcagttc
    58561 cagggaaatg gggctatcgt tgcacaggta tgagttatat gaacttccct aaggttctac
    58621 ttatcacgat gaatgatgga attgatccgg cttcgggtaa acggtttgca ccaagctttg
    58681 gtcattttaa ggatatgaag aacttttctg aattagaaaa tgcttgggat aaaacactaa
    58741 gatatttgac acgaatgagt gttattgttg aaaattctat tgatttatca ttggaacgag
    58801 aagttcctga tattctatgt tcagcattga ctgatgattg tattggtcgt ggaaaacacc
    58861 ttaaagaagg tggagcagta tatgattata tatcaggatt gcaagttgga attgcaaatt
    58921 tgtcggattc attagctgca attaaaaaat tggtgtttga ggaagaacgt ataagcccaa
    58981 gtcagctttg gcatgcactg gaaacagatt atgccggaga agaaggtaag gtcattcaag
    59041 aaatgttgat tcatgatgca cctaagtatg gtaatgatga tgattatgct gacaaattgg
    59101 ttactgctgc ttatgacatt tatgttgatg aaattgctaa atatcctaat acacgttatg
    59161 gaagaggtcc aattggagga attcgttatt caggaacatc ttctatctca gccaacgtag
    59221 ggcagggacg tggaacatta gcaactccag atggacgcaa cgcgggtaca ccgttagcag
    59281 agggttgttc accatcacat aatatggatc aacacggccc tacatctgtt ttaaaatctg
    59341 tttcaaaatt accaacagat gaaatcgtag gtggggttct cttaaatcag aaagtaaatc
    59401 ctcaaacgtt agccaaagaa gaagataaat taaaactaat tgctttgtta cgaacattct
    59461 ttaatcgttt acatgggtac catattcaat acaatgttgt ttccagagag acgctgattg
    59521 acgctcagaa acatcctgaa aaacacagag acttaattgt tcgtgttgca ggatactctg
    59581 cattcttcaa tgttctttct aaggcaaccc aagatgacat tataggacgt actgagcata
    59641 ctttgtaaaa taaagaggtt ctttttatgg aatttatgct tgacacatta aatttagatg
    59701 agattaaaaa gtggtctgaa attttgccgc tagctggggt aacttcaaat cccactattg
    59761 caaaaagaga gggttctatt aatttttttg aacgaatcaa agatgtaaga gaattgattg
    59821 gctctacacc ctctattcat gttcaggtga tttctcaaga ttttgaaggc atcttaaagg
    59881 atgctcataa aattcgaaga caagcaggag atgatatatt tatcaaagta cctgttactc
    59941 cagctggatt acgtgcaata aaggcgctaa aaaaagaggg ctaccatatc actgcaacag
    60001 ctatttatac agttattcag ggattattag ctatcgaagc aggagcggat tacctagctc
    60061 catattataa tagaatggaa aatctgaaca ttgattcaaa ttctgtcatt cgtcaattag
    60121 ctcttgctat tgatagacag aactctccta gtaagatttt agctgcatcc tttaaaaatg
    60181 tagcacaagt aaataatgct ttagctgcag gtgcgcatgc tgttacagca ggagcggatg
    60241 tttttgaatc agctttcgcc atgccatcta tccaaaaggc ggttgatgat ttttctgacg
    60301 attggtttgt tattcaaaat agtcgttcca tttagataga gaggaaatac atatgagaat
    60361 ttttgctagt ccttctagat atattcaggg ggaaaatgcc ttgtttgaaa atgccaaatc
    60421 aattttggat ttgggaaatt gccctattct attatgcgat cagttggttt atgatattgt
    60481 tggaaaacga tttgaagatt acctacatag gtatggtttc catattgttc tggcgctatt
    60541 taatggtgaa gcttctgaca atgaaatcaa tcgagttgtt gccttggctg agaaagaaaa
    60601 ttgtgatagt attatcggtc ttggtggggg aaagacgatt gatagcgcaa aagctattgc
    60661 agatttgatt gaaaagcctg ttattattgc tccaacaatt gcatcgaccg acgcacctgt
    60721 atctgcttta tctgttattt atacagatga aggtgcattt gatcattatc tattttattc
    60781 taaaaatcca gatttagttt tggttgatac aaaagttatt tcacaagccc ctaagcgttt
    60841 attagcgtct ggtattgcag atggtttagc aacttgggtt gaggcgcgtg cggttatgca
    60901 ggcaaatgga aaaactatgt tgggacaaca gcaaacattg gctggagttg caattgcgaa
    60961 gaaatgtgaa gaaacgctgt ttgcagatgg tttacaggct atggcagctt gtgaagctaa
    61021 agtggtgaca ccagcattag aaaatattgt tgaagctaat actttattga gtggtctagg
    61081 ttttgaaagt ggaggattag ctgcggcgca tgcaattcat aatggtttta ctgcattgac
    61141 aggtgacatt catcatttaa cacatggtga aaaagtagct tatggaactt tagtacaact
    61201 attattggaa aatagaccta aagaagaact tgataagtat attgagtttt acaaaaaaat
    61261 tggtatgcca acaactctaa aagaaatgca tttggatcaa gttggatatg atgatttaat
    61321 aaaagttggt aaacaagcaa ctatggaggg tgagacaatt catcagatgc cgtttaagat
    61381 ttcgccttca gatgttgctc aagctattat cgctgtagat gcctatgtaa attcaaaata
    61441 aacaataagg actactgttt tccaaatggt agtcttttat tgatccctgt attgaattct
    61501 atagaagatt gaaataggat gagaacaaat cgattgggaa agtaaaatta atttctataa
    61561 atgttttagc aattgtttcg tactatttca gattcagtct actatatgtt cttcataaat
    61621 caaaaagcga cataggttgt cggcaatcaa aaagcgacat aggttgtcgg ctatttattg
    61681 tgaatacatt aattagcatt ccagttttat cttcggtcta aaataagtat tttgtgctat
    61741 acgagataag cttcttgact tactccttga tttactgcat aacaatggga taaaaagtgg
    61801 gagatagagc aattcatagt catcaaaatt aatgagatac agtatacagt ttttccttta
    61861 aacacatttc aaattccctc aaaaatggta taatagtaac atcacaaaat tggagagaga
    61921 ccatgagttt ttacaatcat aaagaaattg agcctaagtg gcagggctac tgggcagaac
    61981 atcatacatt taagacagga acagatgcat caaaacctaa gttttatgcg cttgatatgt
    62041 tcccttatcc gtctggagct ggtctgcacg taggacaccc agaaggttat actgcaaccg
    62101 atatcctcag tcgttacaaa cgtgcgcaag gctacaatgt ccttcaccca atgggttggg
    62161 atgcttttgg tttgcctgca gagcaatacg ctatggatac tggtaatgac ccagcagaat
    62221 ttacagcgga aaacattgcc aacttcaaac gtcaaattaa tgcgcttgga ttttcttatg
    62281 actgggatcg tgaagtcaac acaacagatc caaactacta caagtggact caatggattt
    62341 tcaccaagct ttacgaaaaa ggcttggcct atgaagctga agtgccagta aactgggttg
    62401 aggaattggg aactgccatt gccaatgaag aagtgcttcc tgacggaact tctgagcgtg
    62461 gaggctatcc agttgtccgc aaaccaatgc gccaatggat gctcaaaatc acggcttacg
    62521 cagagcgctt gctcaatgac ttagatgaac tagattggtc agagtctatc aaggatatgc
    62581 aacgcaactg gattggtaaa tcaactggtg ccaatgtaac tttcaaagta aaaggaacag
    62641 acaaggaatt tacagtcttt actactcgtc cggacacact tttcggtgcg actttcactg
    62701 tcttggctcc tgaacatgaa ttagtagacg ctatcacaag ttcagagcaa gcagaagctg
    62761 tagcagacta taaacaccaa gccagcctta agtctgactt ggctcgtaca gaccttgcta
    62821 aagaaaaaac aggggtttgg actggtgctt atgccatcaa ccctgtcaat ggtaaggaaa
    62881 tgccaatctg gattgcagac tatgtccttg ctagttatgg aacaggtgcg gttatggctg
    62941 tgcctgccca cgaccaacgt gactgggaat ttgccaaaca atttgacctt ccaatcgtcg
    63001 aagtacttga aggtggaaat gtcgaagaag ctgcctacac agaggatggc ctgcatgtca
    63061 attcagactt cctagatgga ctcaacaaag aagacgctat tgccaagatt gtggcttggt
    63121 tggaagaaaa aggctgtggt caggagaagg ttacctaccg tctccgcgac tggctcttta
    63181 gccgtcaacg ttactggggt gagccaattc caatcattca ttgggaagat ggaacttcaa
    63241 cagctgttcc tgaaactgaa ttgccgcttg tcttgcctgt aaccaaggat atccgtcctt
    63301 caggtactgg tgaaagtcca ctagctaact tgacagattg gcttgaagtg actcgtgaag
    63361 atggtgtcaa aggtcgtcgt gaaaccaaca ctatgccaca atgggctggt tcaagctggt
    63421 actacctccg ctatattgat ccacacaata ctgagaagtt ggctgatgag gacctcctca
    63481 aacaatggtt gccagtagat atctacgtgg gtggtgcaga gcatgctgtt cttcacttgc
    63541 tttacgctcg tttctggcac aaattcctct atgacctcgg tgttgttccg actaaggaac
    63601 cattccaaaa actctttaac caagggatga ttttgggaac aagctaccgt gaccaccgtg
    63661 gtgctcttgt ggcaaccgac aaggttgaaa aacgtgatgg ttccttcttc catgtagaaa
    63721 caggggaaga gttggagcaa gcgccagcca agatgtctaa atcgctcaag aacgttgtta
    63781 acccagacga tgtggtggaa caatacggtg ccgataccct tcgtgtttat gaaatgttta
    63841 tgggaccact cgatgcttcg attgcttggt cagaagaagg tttggaagga agccgtaagt
    63901 tccttgaccg agtttaccgt ttgattacaa gtaaagaaat ccttgcggaa aacaatggtg
    63961 ctcttgacaa ggtttacaac gaaacagtca aagctgttac tgagcaaatt gagtctctca
    64021 aattcaacac agctattgcc caacttatgg tctttgtcaa tgctgctaac aaggaagata
    64081 agctttatgt tgactatgcc aaaggcttta ttcaattgat tgcaccattt gcacctcact
    64141 tggcagaaga actctggcaa acagtcgcag aaacaggtga gtcaatctct tatgtagctt
    64201 ggccaacttg ggacgaaagt aaactggttg aagacgaaat cgaaatcgtt gtccaaatca
    64261 aaggtaaagt ccgtgccaaa ctcatggtcg ctaaagacct atcacgtgaa gaattgcaag
    64321 aaattgctct agcggatgaa aaagtcaaag cagaaattga cggtaaggaa atcgtgaaag
    64381 tgattagtgt accaaataaa ttggttaata ttgttgtaaa ataaccaatt tatttgctga
    64441 tttccaacct caaacagttt cccaaactgt ttgagccaac taaactcgtt aatatcgttg
    64501 tgaaataagg taggaatcct tcagagtaga atctggagga ttttttgaat cttcttatga
    64561 aagtatggta tactatgggc aactataaag tttgaaaagt gaaaaaagga gaaagctatg
    64621 ccagtaaatg aatatggtca aatgattggg gagtcaatgg aaggttatac accaggtgaa
    64681 ctgccttcta ttgatttctt agaagggcgt tatgctagga tagaggctct ttcagtggaa
    64741 aagcatgcgg aggatttgct agctgtttat gggcctgata cgcctcagga gatgtggacc
    64801 tacctttttc aggagccagt agcagatatg ggggagctgg ttagcctttt acatcagatg
    64861 ttggctcgta aggaccgttt ttactatgca atcatagaca aggcgactgg taaggctttg
    64921 ggatcttttt ctctcatgcg cattgaccag aataaccgag taatagaagt cggagcagtc
    64981 actttttctc cagagctaag gggtacacgg ataggaactg aagctcagta tctcctagct
    65041 cgctatgttt ttgaggagct aaactatcgt cgctacgagt ggaaatgcga tgccttaaat
    65101 ctgccatcca gacgagctgc ggagcgtttg agctttattt atgaaggaac cttccgtcag
    65161 gcagtggttt ataagggacg tacgagagat acggattggt tgtctatgat tgataaggac
    65221 tggcctcaag tcaaagatcg tttggaaaca tggttgcgtc ctgaaaattt tgataaaaat
    65281 ggacagcagt acaagagctt gagagaactt taagaggtgt tgacatgatt actattaaaa
    65341 agcaagaaat tgtcaagcta gaggatgttt tacatctcta tcaggttgtc ggttggacaa
    65401 attataccca tcaaccagag atgctggagc aggccttatc tcattcatta gtaatttatc
    65461 tggcacttga tggtgatgct gtggtgggct tgattcgttt ggttggagat ggattctcat
    65521 cagtattggt tcaggattta atcgttttac caatctatca gcgtcaagga attggtagtg
    65581 ccttgatgaa agaggcttta gaggattaca aagatgccta tcaagtccag ctggtgacag
    65641 aagagacaga aagaaccttg ggcttctatc gttctatggg ctttgaaatc ttatccacct
    65701 ataattgtat agggatgact tggatgaatc gtaaaaaata acaaaacttg ttttttctta
    65761 agcaaaattt aaggatggtc tagtattata tagtcattaa ataaagacct cctaacttta
    65821 tttaataaaa tcctaaactt ttttcatcac aatctcctaa tgaagccacc caatcaggtg
    65881 gcttttttgc gtggtgaggt gctggtgata gaaatttttt gcaaaatagt aaaattttag
    65941 aaaagttaag ctagttttaa gtttcgtctt gtatcatgta gttattaaat aaagacctcc
    66001 taactttatt taataaaatc ctaaactttt ctttttcata ataatctccc ttaactccac
    66061 ccaatcaggt ggagtttttt agctctattt caggcttttg gggactattc taaaaataat
    66121 ttttcgatat ttttcggtat ttttcggatt ttggtcgggg aattggcggg gactttttta
    66181 gcgaatataa ctaagaaata ggtctgtggt cgcttcagct agttcaaact caatttgatt
    66241 gtaagtgact gttaattgaa gaactggaac tactgtaata atgattttgc ttgtctagta
    66301 gtggcgtatt aaatgagaag tgacatgtaa gattgtctct tcgtttacga aatcaaaatt
    66361 tctatgaaat tgatagaatc cctaacgttc tcttaatctt aaccgtcttg tcgtacagcg
    66421 gttgaagaaa atgtaaacct ttgacaaaat tcgtgaacta tgagataata aaaaggaatt
    66481 gagtactagt tctatatcat aggctagaaa agaccccaag ctcacgacca aataagcttg
    66541 gggtcttttt tgacactatt tgtccttgtt tagccattta tcgactatac gaagaacgac
    66601 accgaccaca attggtccga tgatagtttt gagtactaat tccatcatgg gctatctcac
    66661 ctcctttcgt aggcggtgta gcagtgtcgt agaatattat accacataag tgctaaactt
    66721 aggagtcacc caatcaggtg gcttttttgt ttgtggcttg gttttttgat ataatagagc
    66781 catgagtaga attttagata atgagataat gggggatgag gagttagtag aacgcacgct
    66841 ccgtcctcag tatttacgtg aatatatcgg acaggataag gtcaaggacc agctacaaat
    66901 ctttattgaa gctgccaaaa tgcgggatga agcgctggat catgtgctct tatttgggcc
    66961 tccaggtctc gggaaaacga ccatggcctt tgttattgcc aacgaactgg gagtcaatct
    67021 taagcagacg tcgggtccag tcattgaaaa agccggagat ctggtagcta ttttgaatga
    67081 gttagagcct ggggatgtac tttttattga tgagatccat cgtttgccaa tgtcagtgga
    67141 agaggtgctt tatagtgcta tggaggactt ctacatagat attatgattg gggctggtga
    67201 gggtagtcgt agtgttcatt tggagttacc accttttacc ttgattggtg cgacgactcg
    67261 ggctggtatg ctctccaatc cgctacgggc acgttttggg attacaggcc atatggagta
    67321 ttatgcccat gctgacttga cagaaattgt cgagcggacg gcagatattt ttgagatgga
    67381 aatcactcat gaggcagcat ctgagttggc cctacgtagt cgtgggaccc ctcgtattgc
    67441 caatcgtctc ctcaagcgcg tgcgcgattt tgcccagata atggggaatg gggtaattga
    67501 tgatattatt accgataagg ctttgactat gctggatgtt gaccatgaag gtttggacta
    67561 tgtggatcaa aaaatccttc gtaccatgat tgagatgtac agtggaggac ctgttggtct
    67621 aggaactctt tctgtgaata tcgccgaaga gcgtgagaca gttgaagata tgtatgagcc
    67681 ttacttgatt caaaaaggtt ttatcatgcg gacacggtct ggacgggtgg cgactgctaa
    67741 ggcatatgag cacttaggtt atgaatacag tgaaaaataa gcaagaaatc ttagaggctt
    67801 ttagagaaaa tccagatatg atggccattc tgacgatcat ccgagacctt ggtctgaaag
    67861 actcgtggtt ggcagcaggt tctgtcagaa atttcatttg gaatcttttg tcagacaaat
    67921 ccccttttga tcatgaaaca gatatagatg tgattttctt tgatccagat ttttcttatg
    67981 aggaaacctt attactggag aaaaagctgc gagaggattt tcctcagtac cagtgggaat
    68041 tgaaaaatca ggtctatatg catcagcaca gtcctcacac tgcttcctat accagttctc
    68101 gtgatgctat gagtaagtat ccagaacggt gtacgacagt tggactgcgc ttgaatgaag
    68161 aatcagattt tgaactctat gcaccttatg gtttggagga tattttgaat tttcaagttc
    68221 gtccaactcc tcatttctta gaaaatgaag accgaatgga actctatcaa acacgtttat
    68281 ccaagaaaaa ttggcaggag aaatggaaaa atttgatttt taaaaatact taaggaaact
    68341 ttaagctagg gagtgtacac taagttcata agttaagaag accttaactt aaactcctaa
    68401 aactttttca taataatctc cctataaaaa taaagtcgcc caatcaggcg gcttattttt
    68461 ttgaaaaatg ggcttggtgc ctgagaataa atagcttagt gatagaagaa aatggggaaa
    68521 tatggtataa taaaacgata gatttttgaa taggaataag atcatgtttg gattttttaa
    68581 gaaagataag gctgtggaag tagaggttcc gacacaggtt cctgctcata tcggcatcat
    68641 catggatggc aatggccgtt gggctaaaaa acgtatgcaa ccgcgagttt ttggacataa
    68701 ggcgggcatg gaagcattgc aaaccgtgac caaggcagcc aacaaactgg gcgtcaaggt
    68761 tattacggtc tatgcttttt ctacggaaaa ctggacccgt ccagatcagg aagtcaagtt
    68821 tatcatgaac ttgccagtag agttttatga taattatgtc ccggaactac atgcgaataa
    68881 tgttaagatt caaatgattg gggagacaga ccgcctgcct aagcaaacct ttgaagcttt
    68941 aaccaaggct gaggaattga ctaagaacaa cacaggattg attcttaatt ttgctcttaa
    69001 ctatggtgga cgtgctgaga ttacacaggc gcttaagttg atttcccagg atgttttaga
    69061 tgccaaaatc aatccaggtg acatcacaga ggaattgatt ggtaactatc tctttaccca
    69121 gcatttgcct aaggacttac gagacccaga cttgattatc cgtactagtg gagaattgcg
    69181 tttgagcaat ttccttccat ggcagggagc ctatagtgag ctttatttta cggacacctt
    69241 atggcctgat tttgacgaag cggccttgca ggaagctatt cttgcctata atcgtcgtca
    69301 tcgccgattt ggaggagttt aggaggaaat atgacccagg atttacagaa aagaaccttg
    69361 tttgcaggga ttgccctggc tattttccta ccaattttaa tgattggggg cctcttgctt
    69421 cagatagcaa tcggaatcat agccatgcta gccatgcatg aacttttgaa gatgagaggt
    69481 ctagagacca tgacgatgga gggcctcttg accctctttg caacctttgc attgaccatt
    69541 cccttggaga attacctgac ttttttgcca gttgatggga atgtggttgc ctatagtgtt
    69601 ttgatttcaa tcatgttagg aacgaccgtt tttagcaagt cttatacgat tgaggatgcg
    69661 gttttccctc ttgctatgag cttctacgtg ggctttggat ttaatgcttt actagatgct
    69721 cgtgttgcag gtttggacaa ggctctctta gccttgtgta tcgtctgggc gacagacagt
    69781 ggtgcctatc ttgttgggat gaactatggg aaacgaaagt tagcaccaag ggtatcgcct
    69841 aataaaaccc ttgagggtgc cttgggtggt attttaggag caattttagt aaccattatc
    69901 tttatgatag ttgacagtac agttgctctt ccatatggaa tttacaagat gtcagtcttt
    69961 gctattttct ttagcattgc tggacaattt ggtgatttac tagaaagttc gatcaaacgt
    70021 cattttggtg ttaaggattc tgggaaattt atccctggac atggtggtgt tttggatcgt
    70081 ttcgatagta tgttgcttgt atttccaatc atgcacttat ttggactctt ttaatcaaaa
    70141 gacggaggaa acgctatgct cggaatttta acctttattc tggttttcgg gattattgta
    70201 gtggtgcacg agttcgggca cttctacttt gccaagaaat cagggatttt agtacgtgaa
    70261 tttgccatcg gtatgggacc taaaattttt gctcacattg gcaaggatgg aacggcctat
    70321 accattcgaa tcttgcctct gggtggctat gtccgcatgg ccggttgggg tgatgataca
    70381 actgaaatca agacaggaac gcctgttagt ttgacacttg ctgatgatgg taaggttaaa
    70441 cgcatcaatc tctcaggtaa aaaattggat caaacagccc tccctatgca ggtgacccag
    70501 tttgattttg aagacaagct ctttatcaaa ggattggttc tggaagaaga aaaaacattt
    70561 gcagtggatc acgatgcaac ggttgtggaa gcagatggta ctgaggttcg gattgcacct
    70621 ttagatgttc aatatcaaaa tgcgactatc tggggcaaac tgattaccaa ttttgcaggt
    70681 cctatgaaca attttatctt aggtgtcgtt gttttttggg ttttaatctt tatgcagggt
    70741 ggtgtcagag atgttgatac caatcagttc catatcatgc cccaaggtgc cttggccaag
    70801 gtaggagtac cagaaacggc acaaattacc aagattggct cacatgaggt tagcaactgg
    70861 gaaagcttga tccaagctgt ggaaacagaa accaaagata agacggcacc gactttggat
    70921 gtgactattt ctgaaaaggg gagtgacaaa caagtcactg ttacacccaa agatagtcaa
    70981 ggtcgttacc ttctaggtgt tcaaccgggg gttaagtcag attttctatc catgtttgta
    71041 ggtggtttta caactgctgc tgactcagct ctccgaattc tctcagctct gaaaaatctg
    71101 attttccaac cagatttgaa caagttgggt ggacctgttg ctatctttaa ggcaagtagt
    71161 gatgctgcta aaaatggaat tgaaaatatc ttgtacttct tggcaatgat ttccatcaat
    71221 attgggattt ttaatcttat tccgattcca gccttggatg gtggtaagat tgtgctcaat
    71281 atcctagaag ccatccgccg caaaccattg aaacaagaaa ttgaaaccta tgtcaccttg
    71341 gccggagtgg tcatcatggt tgtcttgatg attgctgtga cttggaatga cattatgcga
    71401 ctctttttta gataatcgag gaatattatg aaacaaagta aaatgcctat cccaacgctt
    71461 cgcgaaatgc caagcgatgc tcaagttatc agccatgctc ttatgttgcg tgctggttat
    71521 gttcgccaag tttcagcagg tgtttattct tatctaccac ttgccaatcg tgtgattgaa
    71581 aaagctaaaa acatcatgcg ccaagaattc gaaaagattg gtgctgttga gatgttggct
    71641 ccagcccttc ttagtgcaga attgtggcgt gaatcaggtc gttacgaaac ctatggtgaa
    71701 gacctttaca aactaaaaaa ccgtgaaaaa tcagacttta tcttaggtcc aactcacgaa
    71761 gaaaccttta cagctattgt ccgtgattct gttaaatctt acaagcaatt gccactcaac
    71821 ctttatcaaa ttcagcccaa gtatcgtgat gaaaaacgcc cacgtaatgg acttcttcgt
    71881 acacgtgagt ttatcatgaa ggatgcttat agtttccacg ctaactatga tagtttggat
    71941 agtgtttatg atgagtacaa agcagcctat gagcgtattt ttactcgtag tggtttagac
    72001 ttcaaggcta ttattggtga cggtggagcc atgggtggta aggatagcca agaatttatg
    72061 gccattacat ctgctcgtac agaccttgac cgctgggttg tcttggacaa gtcagttgcc
    72121 tcatttgacg aaattcctgc agaagtgcaa gaagaaatca aggcagaatt gctcaaatgg
    72181 atagtctctg gtgaagatac cattgcttac tcaagtgagt ctagctatgc agctaactta
    72241 gaaatggcaa caaacgagta caaaccaagc aaccgtgttg tcgctgaaga agaagttact
    72301 cgtgttgcaa cgccagatgt taaatcaata gatgaagttg cagctttcct caatgttcca
    72361 gaagaacaaa cgattaaaac cctcttctac attgcagatg gtgagcttgt tgcagccctt
    72421 ctagttggaa atgaccaact caacgaagtc aagttgaaaa accacttagg agcagatttc
    72481 tttgacgttg ctagcgaaga agaagtggcg aatgttgttc aagcaggatt tggttcactt
    72541 ggaccagttg gtttgccaga gaatattaaa attattgcag atcgtaaggt gcaagatgtt
    72601 cgcaatgcag ttgtcggtgc taacgaagat ggctaccact tgactggtgt gaacccaggc
    72661 cgtgatttta cttcagaata tgtggatatc cgtgaagttc gtgagggtga aatttcccca
    72721 gatggacaag gtgtccttaa ttttgcgcgt ggtattgaga tcggtcatat tttcaaactc
    72781 ggaactcgct attcagcaag catgggagca gatgtcttgg atgaaaatgg tcgtgctgtg
    72841 ccaatcatca tgggatgtta cggtatcggt gtcagccgtc ttctttcagc agtgatggag
    72901 caacacgctc gcctctttgt taacaaaacg ccaaaaggtg aataccgtta cgcttgggga
    72961 atcaatttcc ctaaagaatt ggcaccattt gatgtgcatt tgattactgt taatgtcaag
    73021 gatgaagaag cgcaagcctt gacagaaaaa cttgaagcaa gcttgatggg agctggttat
    73081 gaagtcttga cagatgaccg taacgaacgt gtcggtgtta aatttagcga tagtgacttg
    73141 attggtcttc caatccgtat cactgttggt aaaaaagcgg ccgatggcat tgtagaagtt
    73201 aagatcaagg caactggtga caccattgag gttcatgcag ataacgtgct tgaaacgctt
    73261 gaaatcctaa gtaagaaata aaaactataa tcagaagaaa aacaaggaaa aaatgtaact
    73321 agtttttacc ttgttttttc tgtataatgg gagaaatgag tagataaaga ggtaaatgta
    73381 tgctaagatt tccaaaggat tttgtctggg gatcctctac ttctggaccg cagacagaag
    73441 gacgtgtagc tggtgacggt aagggagaca atctctggga ttactggttc caagtggagc
    73501 caaatcgtta ctataatggg attggttcag ataagacatc gactttttat gaaaattggg
    73561 agcgggatat tgagcttttg ttagagactg gtcacacagc ctttcggact tctattcagt
    73621 ggtcacggat ttttccacaa ggctgtggaa aagtcaaccc tcaaggtgtg gatttttatc
    73681 gtaaggtctt tgaggctatt aaggctaaag gaattcgtct gttagtcaat ctctatcatt
    73741 ttgatttacc ttttgccctt caagaggatg gtgatggttg ggaaaataag gcgacagttt
    73801 cagcctatga agactatgct cgtttttgtt ttgagactta tggagattta gtggatcaat
    73861 ggattacctt taacgagccc atcgttcctg tagaatttgg ttatttttat gatgcccatt
    73921 atccacataa ggtggatgca gaggcagctg ttaaggtagc ctatcataca caattggcca
    73981 gcagccgggc tgttaaggct tgccatgaac ttttgcctga ttccaagatt gggattgtcc
    74041 tcaacttgac accggcttat ccacgtagcc agcatcctgc tgatgtcaag gcagctcgta
    74101 ttgcggccct ttttcaggcc caatctttct tagatccatc tgtcttgggg acttatccac
    74161 aggagttggt agaaatcttg catgaacacg gtcttttacc tgatgctaca gaggaagagt
    74221 tggaactcat tcgtgataat acggtggact tccttggtgt gaactactat caacctttgc
    74281 gtgttatggc tcctcgattt gctaagcatc cagagagtcc actcttacca gaacattttt
    74341 acgagcatta tgtgatgcct ggacgtaaaa tcaattctca ccgtggttgg gagatttatg
    74401 agcaagggat ttatgacatc gcccaaaata tcaaggaaaa ttatggcaat attgagtgga
    74461 tgttgactga gaatggtatg ggtgttgaag gggaagaaaa attccgtcaa gatggaatga
    74521 ttcaagatga ttaccgtatt gactttgtaa aaggtcatct tcgtgaactt caccgtgcca
    74581 ttgaagatgg tgccaactgt aagggctact tgatttggac ctttattgac tgctggtcat
    74641 ggctcaacag ctataaaaat cgctatggtt tggtcgaatt agacttggaa acgcaagaac
    74701 gtcgtctgaa gaaatcaggc cactggttca aagaattaag cgataataat ggattttaaa
    74761 aaggactctg actgattatc ccaatcggtc agagttttta tgcttacagg agcttaattt
    74821 gaactatacc aatttttact cttgacaagt gaaagaaaca agtatatact gtttttactg
    74881 attctataaa agatgaatta gactatacca attttaagga gaaagcacag cttgcctgtg
    74941 tcgtatatac tatgtgtgga attgttggtg ttgttggaaa cacaaatgca actgatattt
    75001 tgattcaagg gcttgaaaag cttgaatacc gtggctatga ttctgcggga atttttgtcc
    75061 tagatggtgc tgataaccat ttggtgaagg cggttggtcg tattgcagaa ttgtctgcca
    75121 agacagctgg tgttgaggga acaactggta tcggacatac tcgttgggct actcacggaa
    75181 aaccaactga ggacaatgct cacccacacc gctctgagac agaacgtttt gtcttggtgc
    75241 ataatggggt gattgagaac tatcttgaaa tcaaggaaga ataccttgca ggtcaccact
    75301 tcaaggggca gacagatact gaaatagccg ttcatttgat tggaaaattt gcggaagaag
    75361 aagggctctc agttcttgaa gccttcaaaa aagctcttca tattatccgt ggttcatatg
    75421 cctttgcctt gattgactct gaaaatccag atgtcatcta tgtagcgaaa aacaaatctc
    75481 cacttttgat tggtcttggg gaaggctaca atatggtctg ctcagatgct atggctatga
    75541 ttcgtgaaac caaccaatac atggaaattc atgaccaaga gttggtaatc gtcaaggctg
    75601 atagcgtgga agttcaagac tatgatggta acagtcgtga acgtgctagc tatactgcgg
    75661 aacttgactt gtcagatatc ggtaagggaa cttatcctta ctacatgctt aaggaaattg
    75721 atgagcaacc aactgttatg cgtaaactca ttcaagccta cacggatgat gctggtcaag
    75781 tagtgattga tcctgctatc attaaggctg ttcaagacgc agaccgcatc tacatccttg
    75841 cagctggaac atcttaccat gcaggatttg cttctaagaa aatgttggaa gaattgacag
    75901 atacaccagt tgaacttgga atctcatctg agtggggcta cggtatgcca cttctcagca
    75961 agaaaccact cttcatcttt atcagccaat ctggtgaaac agcggatagt cgtcaagttt
    76021 tggtcaaggc taatgaaatg ggaattccaa gcttaacagt gacaaacgtt ccaggttcaa
    76081 ccctctcacg tgaagccaac tataccatgc tccttcacgc aggtcctgaa attgccgtgg
    76141 catcaactaa agcctataca gcgcaaatcg cagcccttgc cttccttgca aaagcagtcg
    76201 gagaagcaaa tggtaatgct aaagcgcaag cctttgacct ggttcatgaa ttgtcaatcg
    76261 tagctcagtc tatcgaatca actctttcag agaaagaaac cattgaagcc aaggttcgtg
    76321 aacttcttga aacaactcgt aacgcctttt acatcggacg tggtcaagat tactacgtag
    76381 ccatggaagc aagtctcaaa ctcaaagaga tttcttatat ccagtgtgaa ggttttgcgg
    76441 caggagaact caagcacgga accattgcct tgattgaaga aggaacgcct gtcttgtctc
    76501 tcttgtcaga tccagttctt gccaaccata ctcgtggaaa tatccaagag gtcgcagccc
    76561 gtggtgccaa agtcctcact atcgcagaag agaatgtagc caaagatacc gacgatatcg
    76621 tccttacgac cgtacatcca tacctctcac caatttcaat ggtcgtacca acgcaattag
    76681 tcgcttactt tgcaaccctc caccgtggct tcgatgtgga caaaccacgt aaccttgcca
    76741 agtcagtaac ggtagaataa gctaaaaaag tctagttatc taggcctttt tcttgtgagc
    76801 aaattggttg cttgtactca agattcgtgt tatactcttc gaaaatctct tcaaaccacg
    76861 tcaacgtcgc cttgccgtag gtatggttac tgacttcgtc agttctatcc acaacctcaa
    76921 aacactgttt tgagcagcct gcagctagct tcctagtttg ctctttgatt ttcattgagt
    76981 attagaataa tttttcaaat tgaagtacag agatagataa ggagaatcac aatggtagaa
    77041 ttgggaattt caacatttgg ggaaataacg gagcttgaag ggactggaca aacttacagt
    77101 catgctgaac gcattcgcca gttggtggca gagattgagc tggctgacaa ggttggtttg
    77161 gatgtatatg ggattggtga gcaccatcgg gcggattttg cagtatcagc cccagagatt
    77221 gttctggccg ctggggcagt caataccaag aaaatccgtt tgaccagtgc agtcagcatt
    77281 ctgtcaagca tggacccgat tcgtttgttc caacagtatg ccactatcga tgctttgtca
    77341 aatggacgag cggagattat ggctggaagg ggctctttca cggaatcttt ccctctgttt
    77401 ggttatgact tgaaagacta cgactctctt tttgatgaga aattagcctt gcttcagtta
    77461 gtcaatgaaa agaccaagtt agactggcaa ggtcgattga ctcaaacgat tgctggtaaa
    77521 gaagtttatc ctcgtccagt tcaggacaaa ttgcccttgt ggatagctac aggtggtcat
    77581 gtcgaatcaa cagtgaagat tgctcaggct ggtctaccga ttgtatatgc tattattggt
    77641 ggtaatccac gttattttaa aaagttgatt caggcttatc gtgagattgg aagcgaagcg
    77701 ggtcatgctg acaaagactt gaaagtggga gcccattctt gggggtggat tgcggaagat
    77761 ggtgagcagg cggtgaaaga ttatttccat ccgactaagc aagtggtgga tgctatttcc
    77821 aaagaccgtc cgcactggca ggaattgcgt tatgagcaat atttggagca ggtagggcca
    77881 aatggcgcca tgtttgttgg caatccagat caggtggccg aaaaattgat tcgcatgata
    77941 gaggatttag gattggaccg cttcatgctt catctaccgc ttggttctat gcctcatgac
    78001 caggttctga gagctattga actcttcgga acacaagttg cacccaaagt acgagcttat
    78061 tttgctatga aagaggctta ataaaaaatc ctaatcaagt ttattaggac aacaaatatt
    78121 gtacgaaaat taatcccccg agctataatg cttgggggat ttttctatag gagggctagt
    78181 ttagttctct tttttatttt ttagtaagaa accgagacca aggagcgcaa ggaggctgat
    78241 tcctgcaaat aggagtttgt tttcgttttt gattcctgta tttggaagtt cagcttgtct
    78301 atctggggtt gcaggtatat ttttcttgct agagttttct accgattcgt tttgaaccgc
    78361 ttcttttaca gatgatgctt gtgcttcttg tgctggttgt tcagcttgtg aatcagctgt
    78421 agcttggcta ggtttatttt ccgcatcagc tactttggca tctggtttag tagaatcagg
    78481 tctagcttct ggagctgggt cttgatgttc aggcttggaa ggggttgagt ctggtttctc
    78541 ttctgcagtt gactcatggc tagttccatt ttgagagact cgaagaacag tagctgtaag
    78601 ggcattcaat ttcaagcctt tttcagtcca ttcaagtcct ttcgggttgg caattccgac
    78661 tggtcctgct tggttttcat ctgccaaaac ttccgcattt cttagatgtg caaaggcagt
    78721 tcccaaatta aattcgcgag ctttttcatc cgcattgaca aagactgcgt agatatcgcc
    78781 gtttggagca gtgatttggt agccaatcac tacatcctct ttttccacac cattttggcc
    78841 tgggacagtg atgaggtgga cacggtcttt gatatcttgg agactcttaa gtcggaaggc
    78901 atctgtagat tgacgaaggg caatcaaacc tttcatatag tcacggctct tgacattttc
    78961 aggataagct ttaccatctg tagccttagt ccagtcaaac ttgttgactg cgtcgctaga
    79021 atcgtaagaa tcatggatga agtaaggata gtcaaatggg ttgccgtcct tatcacgcaa
    79081 caagtgagat ttgtttggaa ccttatcctc tgctactgga gtcttgtagg ctgggtcacg
    79141 gaattgttta gtacgtccat attcctgacc ggagtggata aatggagttc cttgagctgt
    79201 caagaccatg agatttccaa gtcgtaaacg acggtggatt tcagcatagt tctcagcctt
    79261 gcttgggtct tttttgatag actgggcaat gatgtcaaag agggtcaagt tatcatgggc
    79321 tgcgatgtat tggatgacat ctccagggct gtcagcttca aagttagttg gttgagcaat
    79381 gagattttta aagatggtgt tgacatcacg cttgccacct gtgataaagg caggttgacc
    79441 ttcgtttgga taaccagatt tgaggttgtt acggatgtca tctgaaaaga cagcgacagt
    79501 atcggtatgt ttcatccaat cttggtcagc agctttagta ggcatgtttt catcaccggc
    79561 ataggttctc caaccttcgc ctaacatgat tagatttgga ttgagggcgc gtgcagcctt
    79621 gtaagcttct tcgatagaag cggcgtcatg gtctcccatc atatcgaagc ggaagccatc
    79681 cactttgtag gtatcaacta ggtatttgat agagtcaact aggaggcgtt tggtcatatg
    79741 gtgggttgtc cccaagcgtc caccaccaaa gctagttcga ggtgtgccat cggcatccat
    79801 aaagtggtag tagtttggct ctaggtcttc aaagatatcg actttggctg tgtggttata
    79861 aacgacatct aggatagctc ccataccacg tttgtggatt tcgttgatga ggtttttaaa
    79921 ttctgcgatt cgtttttctg gattcttagg atcgcttgag tacataccag tcaaggagaa
    79981 gtagttttga gggtcatatc cccagttgta gttgctgttg cttgaagcat agtcagacaa
    80041 gcgttcatgg tttttcaatt cattgacaaa gtagtaagac aagactggaa ggagctggat
    80101 atgggttaca cccaagtctt tgagatagtc tagtttttca atgaaggctt caaaagtccc
    80161 aaatggtttg gtcaagtctt ttgcaatggc aggatctgaa gtgaagtcac gcacatgagc
    80221 ttcgtagata acggcgtctt cacgagtctt gaaattgtga atcttaccat aagtcaagtc
    80281 ttgaggtccg agtttagctg gatctacaaa ggcggcttta gccactttat gggcatcgtc
    80341 aatcttggca tcgttgctat tccaagcagc aagagattta gcgtaaggat cgagtgcaag
    80401 aacagtttta ccttgacgct cgatttggta ttgataatag tagccagtga aatctgtgat
    80461 tccgagtttg tttgtgctgt ctagagtttg tttccaagtt cctctttccc ctttttcgag
    80521 agcgacagtt ccaactactt tgtcagggtc attcttgtcg tagacaacaa cagaaacctt
    80581 atcagcactt ggtgaccaaa gggtcaaatc aacttgtttt ccttcttctt ttaggtcagc
    80641 tcccagtttg ccatcatagc tgtatgtctc atctttcagg cgccagcttg ttttggtagt
    80701 gaattggtcg gaattgtagc taacagtata aggatgtttt gtgtcagaga aatctccgct
    80761 gtaggtcact ttcttaccag cttcatcgat tgcaacatcg gtaatagtta ccttgtttcc
    80821 taggtgatta gtgatgttgg agtgtttgag gatatcttct tttttagcac cgacaagtgt
    80881 tgaaaagcta ctttcaatgc tagaagtgcc tacgtgttgg gctcctgtca tacggatatc
    80941 atggacatag tatggatttg tgtaaatcga ttcatcatcg tcttttagga aaatttggct
    81001 atgatttttc aaatctgtga acttataatt ttctttacgg attttcacgt cgtctccttg
    81061 tttgctctca tctagtaata aaaatccaaa ttctcttgcg gcttcattaa gaggaatgtc
    81121 gatatagcgg ccatatttgc ctgtagccgt aaagtctgtt ccgtcaggcc attgagcgct
    81181 acttggattt ttcacatctc cccagtacca gagagatttc ttgtcatagt tgccatctgt
    81241 gcggtagtag ttgacgcgaa cagttcctgc aggctgtggc tcgtaagaga aaaccttgta
    81301 atcttggtct aaccaagctt cgttcatttt tggaactagt ttttctacag atttatcgcc
    81361 ggttagattt tttccagctg tattgttgat gaggaagcta attttcttgg cttgttctcc
    81421 ctttaatttg acatctaggt aatagccgta gtcatctttc ttggcatcct tgaaggacaa
    81481 agctccgttt ggccagtttt cagatggttt ttcaacatcg tcccaagtcc atagtccttg
    81541 agcatccttg ttttcttcag gaagtttttt gacatggata cggaagtagt tatcttcgat
    81601 gggctcttct gccttagttt cagttgttac tggactagta gaagtagttg gctcacttga
    81661 acgatcttgt ccagtttgtg gtgctggatc ggctgggctt gcagcaggag ttgttggctc
    81721 tgttgcaggt ttttcttctg ttttctctag ggaagcgttt gcatttccaa gtgaagaaat
    81781 atcacttttg ttcttaccag agccagctgc tgtatcagta agaggttggg caagggtggt
    81841 tgtatttctg ttgttgggtt cgttagtggt tggtgtactt tcgtcggatg agatagttgg
    81901 tgtagccatg gcaagcaaga caaggcttgc gccgataagg acagaaccag tcccattttt
    81961 gagggaacgg atgctgtaga ccattttttt ctcagtgtga gatggtgttt ttctcataat
    82021 gattctccaa tcaataaatt tctatatcag tatagcatgt agtaaaacaa tatgcaagcg
    82081 ttttctttga tttgtgaaaa aaattcttac ttttgagaag ttttctgtac tattttatat
    82141 atcagtataa atgacggaga taaaaatgtc acataagcca agccgtatta ttaggaagat
    82201 gatgtgttcc cagctatttt tattcagaaa ttgatctcta acattaccaa taaaaaagaa
    82261 aaatatcttg acaaacaagg gaaaattttg ttacaataat agacggtact ttttactttt
    82321 ggtctctcaa gagtgtacag ggacgtgctg acaaatgttg caaaagtaca cacagatgat
    82381 atctgtcacc aagtgtatca tcaccaaaaa taaaaaaaca caggagaatg tagatgccta
    82441 caattaacca attggttcgc aaaccgcgta aatcaaaagt agaaaaatct aaatcaccag
    82501 ctttgaacgt tggttacaat agtcataaaa aagttcaaac aaacgtttct tcaccacaaa
    82561 aacgtggtgt tgcaactcgt gttggaacaa tgacacctaa aaaacctaac tcagcccttc
    82621 gtaaattcgc tcgtgtacgt ttgagcaacc ttatcgaagt tactgcctac atcccaggta
    82681 tcggacacaa cttgcaagag cacagcgtgg tgcttcttcg cggtggacgt gtaaaagacc
    82741 ttccaggggt acgttaccat atcgtccgtg gtgcacttga tactgcaggt gttaacgatc
    82801 gtaaacaagg tcgttctaaa tacggtacta aacgtccaaa agcataagga aaggggataa
    82861 agagaaatga gtcgtaaaaa tagagctcca aaacgtgacg tattgccaga tccgctatac
    82921 aattcacaac tagttactcg tcttatcaac cgcgttatgc ttgatggtaa acgtggtact
    82981 gctgcttcaa tcgtttacgg tgcttttgag caaatcaaag aagctactgg caacgatgca
    83041 cttgaagtat ttgaaacagc tatggaaaac atcatgcctg tacttgaagt acgtgcacgt
    83101 cgtgttggtg gttctaacta ccaagtccca gttgaagttc gtccagaacg tcgtacaaca
    83161 cttggacttc gttggttggt aacaatcgct cgtcttcgtg gtgaacacac aatgcaagac
    83221 cgtcttgcaa aagaaatctt ggatgctgct aacaacactg gtgcagctgt taagaaacgt
    83281 gaagatactc accgtatggc tgaagctaac cgtgcattcg cacacttccg ttggtaagat
    83341 aggatgcgaa agcgttaaga aagtcccaga gaaaataggg aatcgaagca ggttgcggtt
    83401 gcaaccaatg agattcatct ttttctccag acttttagct tgagctcaac taaatcatga
    83461 tgctaggcac ggtaaggatg caaggtgaaa ataggaaact gacgcagtat tcaacgaata
    83521 caaggagttt tatctttttc acgcagcatc ccgttccagc tcacatcggc taactaactt
    83581 tagcccgggt tcaaattagc taaatcgatt agtattagct ataactcagc ttaccatctc
    83641 gtaagttgaa accaacaata gcatgaaaac attgagaacg ggtaggtcct gcctatccgt
    83701 ttttattaaa atcgtgttat aatagaatag aaatcaaaaa taaataggag aaacaaacct
    83761 catggcacgc gaattttcac ttgaaaaaac tcgtaatatc ggtatcatgg ctcacgtcga
    83821 tgccggtaaa acaacaacta ctgagcgtat tctttactac actggtaaaa tccacaaaat
    83881 cggtgaaact cacgaaggtg cgtcacaaat ggactggatg gagcaagagc aagaacgtgg
    83941 tatcacgatc acatctgctg cgacaacagc tcaatggaac aaccaccgcg taaacatcat
    84001 cgacacacca ggacacgtgg acttcacaat cgaagtacaa cgttctcttc gtgtattgga
    84061 tggtgcggtt accgttcttg actcacaatc aggtgttgaa cctcaaactg aaacagtttg
    84121 gcgtcaagca actgagtacg gagttccacg tatcgtattt gccaacaaaa tggacaaaat
    84181 cggtgctgac ttcctttact ctgtaagcac acttcacgat cgtcttcaag caaatgcaca
    84241 cccaatccaa ttgccaatcg gttctgaaga tgacttccgt ggtatcattg acttgatcaa
    84301 gatgaaagct gaaatctata ctaacgacct tggtacggat atccttgaag aagacatccc
    84361 agctgaatac cttgaccaag ctcaagaata ccgtgaaaaa ttgattgaag cagttgctga
    84421 aactgacgaa gaattgatga tgaaatacct cgaaggtgaa gaaatcacta acgaagaatt
    84481 gaaagctggt atccgtaaag cgactatcaa cgttgaattc ttcccagtat tgtgtggttc
    84541 agccttcaaa aacaaaggtg ttcaattgat gcttgatgcg gttatcgact accttccaag
    84601 tccacttgac atcccagcaa tcaaaggtat taacccagat acagacgctg aagaaactcg
    84661 tccagcatct gacgaagagc catttgcagc tcttgccttc aagatcatga ctgacccatt
    84721 cgtaggtcgt ttgacattct tccgtgttta ctcaggtgtt cttcaatcag gttcatacgt
    84781 attgaatact tctaaaggta aacgtgaacg tatcggacgt atccttcaaa tgcacgctaa
    84841 cagccgtcaa gaaatcgaca ctgtttactc aggtgatatc gctgctgccg ttggtttgaa
    84901 agatactaca actggtgact cattgacaga tgaaaaatct aaaatcatcc ttgagtcaat
    84961 caacgttcca gaaccagtta tccaattgat ggttgagcca aaatctaaag ctgaccaaga
    85021 caagatgggt atcgcccttc aaaaattggc tgaagaagat ccaacattcc gcgttgaaac
    85081 aaacgttgaa actggtgaaa cagttatctc aggtatgggt gaacttcacc ttgacgtcct
    85141 tgttgatcgt atgcgtcgtg agttcaaagt tgaagcgaac gtaggtgcgc ctcaagtatc
    85201 ttaccgtgaa acattccgcg cttctactca agcacgtgga ttcttcaaac gtcagtctgg
    85261 tggtaaaggt caattcggtg atgtgtggat tgaatttact ccaaacgaag aaggtaaagg
    85321 attcgaattc gaaaacgcaa tcgtcggtgg tgtggttcct cgtgaattta tcccagcggt
    85381 tgaaaaaggt ttggtagaat ctatggctaa cggtgttctt gcaggttacc caatggttga
    85441 cgttaaagct aagctttatg atggttcata tcacgatgtc gactcatctg aaactgcctt
    85501 caagattgcg gcttcacttt cccttaaaga agctgctaaa tcagcacaac cagctatcct
    85561 tgaaccaatg atgcttgtaa caatcactgt tccagaagaa aaccttggtg atgttatggg
    85621 tcacgtaact gctcgtcgtg gacgtgtaga tggtatggaa gcacacggta acagccaaat
    85681 cgttcgtgct tacgttccac ttgctgaaat gttcggttac gcaacagttc ttcgttctgc
    85741 atctcaagga cgtggtacat tcatgatggt atttgaccac tacgaagatg tacctaagtc
    85801 agtacaagaa gaaattatta agaaaaataa aggtgaagac taatccgtcc tcactctaga
    85861 aggaagtcac ttagtggctt ccttttgtct ttagaaaata cctctaaata tggtaaaata
    85921 gtagaagaat aatgtgagga aaatgaatgt caaatagttt tgaaattttg atgaatcaat
    85981 tggggatgcc tgctgaaatg agacaggctc ctgctttagc acaggccaat attgagcgag
    86041 ttgtggttca taaaattagt aaggtatggg agtttcattt cgtattttct aatattttac
    86101 cgattgaaat ctttttagaa ttaaagaaag gtttgagcga agaattttct aagacaggca
    86161 ataaagctgt ttttgaaatt aaggctcggt ctcaagaatt ttcaaatcag ctcttgcagt
    86221 cctactatag ggaggttttc tctgaaggtc catgtgctag tcaaggtttt aagtcccttt
    86281 atcaaaattt gcaagttcgt gctgagggta atcagctatt tattgaagga tctgaagcga
    86341 ttgataagga acattttaag aagaatcatc ttcctaattt agccaaacaa cttgaaaagt
    86401 ttggttttcc aacttttaac tgtcaagtcg agaagaatga tgtcttgacc caagagcagg
    86461 aagaggcctt tcatgctgaa aatgagcaga ttgttcaagc tgccaatgag gaagcgctcc
    86521 gtgctatgga acaactggag cagatggcac ctcctccagc ggaagagaaa ccagtctttg
    86581 attttcaagc gaaaaaagct gcagctaaac ccaagctgga taaggcggag attactccta
    86641 tgatcgaagt gacgacagag gaaaatcgtc tggtatttga aggggttgtt tttgatgtgg
    86701 agcaaaaagt gactagaaca ggtcgtgttt taatcaactt taaaatgacg gactatactt
    86761 caagtttttc tatgcaaaag tgggttaaaa acgaggaaga ggcccagaag tttgacctca
    86821 tcaagaagaa ttcttggctc cgagttcgag ggaatgtgga gatgaataat ttcacacgcg
    86881 atttgactat gaacgtacag gatctgcagg aagttgttca ctatgagcgg aaggatttga
    86941 tgccagaagg tgagcgtcgg gttgagtttc atgctcatac taacatgtcg actatggatg
    87001 ctttgccaga ggtcgaagag attgttgcaa cagctgctaa gtggggacac aaggcggttg
    87061 ctatcacgga ccatgggaat gtccagtcct ttccacatgg ctataaggcg gctaagaaag
    87121 cgggaatcca gctgatctat ggaatggaag ccaatatcgt ggaggaccgt gtccctatcg
    87181 tctataacga agtggagatg gacttgtcag aagcaaccta cgtggtcttt gacgtggaaa
    87241 cgacgggact ttcagctatc tataatgact tgattcaggt tgcggcctct aagatgtaca
    87301 aggggaatgt tattgctgaa tttgatgaat ttatcaatcc tgggcatccc ttgtcagctt
    87361 ttactacaga gttaactgga attacagatg atcatgtcaa aaatgccaaa ccactagaac
    87421 aagttttgca agaattccaa gaattttgca aggatacggt cctagttgcc cacaatgcta
    87481 cctttgacgt tggctttatg aatgctaatt atgagcgtca tggtcttcca aagattagtc
    87541 agccagttat tgatacgctg gagtttgcta gaaacctcta tcctgagtat aaacgccatg
    87601 gtttggggcc tttgaccaag cgttttggtg tggccttgga acatcatcac atggccaact
    87661 acgatgcgga agcgactggt cgtctgcttt tcatctttat caaagaggta gcagaaaaac
    87721 atggtgtgac cgatttagct agactcaaca ttgatctaat cagtccagat tcttacaaaa
    87781 aagctcggat caagcatgcg accatctatg tcaagaatca ggtaggtcta aaaaatatct
    87841 ttaagctggt ttccttgtct aataccaagt attttgaagg agtgccacgg attccgagaa
    87901 cggttctaga tgcccatcga gagggcttga ttttaggttc agcctgttca gagggtgaag
    87961 tttttgacgt ggtcgtttct caaggtgtgg atgcggcggt tgaggtggcc aagtattatg
    88021 actttatcga ggtcatgcca ccggctatct atgcgccctt gattgccaaa gagcaggtca
    88081 aggatatgga ggaactccag accattatca agagtttgat agaggttgga gatcgccttg
    88141 gcaagcctgt tctggctacg ggaaatgttc actatatcga accggaagaa gagatttatc
    88201 gtgaaattat cgtccgtagt ttgggacagg gtgcgatgat taaccgaact atcggtcatg
    88261 gtgaacatgc ccaaccagca ccacttccaa aggctcattt tcgaacgact aatgagatgt
    88321 tggatgaatt tgcctttttg ggagaggaac tggctcgtaa actggttatt gaaaacacca
    88381 atgccttggc agaaatattt gaacccgttg aagtcgttaa gggtgacttg tatacgcctt
    88441 tcatcgacaa ggctgaagaa acagttgctg agttgaccta taagaaagct tttgagattt
    88501 atggaaatcc gctgccagat attgttgatt tgcggattga aaaagaatta acatccatac
    88561 tggggaatgg atttgctgtg atttatctgg catcgcagat gctggtgcaa cgttctaatg
    88621 aacggggtta tttggttggt tctcgtgggt ctgtcggatc tagtttcgtt gcgaccatga
    88681 ttgggattac ggaagtcaat cctctctctc ctcactatgt ctgtggtcag tgtcagtaca
    88741 gtgagtttat cacagatggt tcgtacggtt caggatttga tatgccccat aaggactgtc
    88801 caaactgtgg tcacaaactc agtaaaaacg gacaggatat tccgtttgag accttccttg
    88861 gttttgatgg ggataaggtt cctgatattg acttgaactt ctcgggagaa gatcagccta
    88921 gcgcccactt ggatgtgcgt gatatctttg gtgaagaata tgccttccgt gcgggaacgg
    88981 ttggtacggt agctgccaag actgcctatg gatttgtcaa gggttacgag cgagattatg
    89041 gtaagtttta tcgtgatgca gaagtagaac gcctcgctca aggagcggcg ggtgtcaagc
    89101 ggacaacagg ccaacacccg gggggaatcg ttgttattcc gaactacatg gatgtctacg
    89161 attttacgcc tgtccagtat ccagcagatg atgtcacggc tgaatggcag accactcact
    89221 ttaacttcca cgatatcgat gagaacgtcc tcaaactcga tgtactggga catgatgatc
    89281 cgaccatgat tcgaaaactt caggatttgt ctggtattga ccctaataaa attcctatgg
    89341 atgacgaagg cgtgatggca ctcttttctg ggactgatgt gctaggggta acacctgaac
    89401 aaattggaac gcctacgggt atgttgggga ttccagagtt tggaacaaat ttcgtacgtg
    89461 gaatggtaga cgaaacccat ccgacaacct ttgcggaatt gcttcagctg tctggtctgt
    89521 cccacggtac tgacgtttgg ttggggaatg ctcaggatct gattaagcaa ggaatagcgg
    89581 acctatcgac tgttatcggt tgtcgggacg acatcatggt ttacctcatg catgcgggtc
    89641 tggaacctaa gatggccttt accattatgg aacgggtacg taagggtttg tggctaaaga
    89701 tttcagaaga ggagagaaat ggctatatcg aagcaatgaa ggctaataag gtgccagagt
    89761 ggtatatcga atcctgtggg aaaattaagt acatgttccc taaggcccat gcggcagcct
    89821 acgttatgat ggccttgcgt gtagcttact tcaaggttca ccatcctatt tattactact
    89881 gtgcttactt ctccattcgt gctaaggctt ttgatatcaa gaccatgggt gcgggcttgg
    89941 aggtcatcaa gcgcagaatg gaagaaatct ctgaaaaacg gaagaacaat gaagcctcta
    90001 atgtggaaat cgatctctat acaactcttg agattgtcaa tgagatgtgg gaacgaggtt
    90061 tcaagtttgg taaattagat ctctactgta gtcaggcgac agagttcctc atcgacgggg
    90121 atacccttat cccaccattt gtagcaatgg atggtctggg agagaacgtt gccaagcaac
    90181 tggtgcgggc gcgtgaagag ggagaatttc tctctaaaac agaactacgc aagcgtggtg
    90241 gactctcatc aaccttggtt gaaaagatgg atgagatggg tattcttgga aatatgccag
    90301 aggataacca gttgagtttg tttgatgagt tgttttaaaa aattgcttaa taatctatta
    90361 aaagaggcta acgtatatcc aatagattta cattagcttt cttttttgtt aaaatagtct
    90421 atggaaagag ggtgagagta tgtcaaagat gagtataagc atccgtctgg atagtgaggt
    90481 taaggagcag gcccaacagg tgtttagtaa tctgggaatg gatatgacaa cagctattaa
    90541 tattttcctt cgtcaggcaa ttcaatatca gggactacct tttgatgtta gactagacga
    90601 aaatcggaag ttgctccaag cgttaacgga tttagaccaa aatcgtaata tgagccagtc
    90661 ttttgaatca gtctcagatt tgatggagga cttacgtgct taagattcgt tatcataaac
    90721 agtttaaaaa agattttaag ttggctatga agcgtggttt gaaggcagaa ttattagaag
    90781 aagttttgaa ttttctggtt caagaaaaag aacatcctgc cagatatcgt gatcattcat
    90841 tgacggcatc caagcatttt caaggagttc gtgaatgcca tacccagcca gattggcttt
    90901 tggtttataa agtagacaag tcggaattga ttttaaattt gctgaggaca ggcagtcaca
    90961 gtgatttatt ttaatctatt ttaagggggt tctcatgaaa ctaagaatat ttgcggaaga
    91021 taagccggct aagaaggtat ttgaatatca attagaactt gctgatcgta caattcttct
    91081 atcgacagca ctcttgtcag gtgctattgc tttagcagga atcttttctg ctttgagaga
    91141 aaaataaaaa tagaaaagag aaaacagaat ggttttacca aattttaaag aaaatctaga
    91201 aaaatatgcg aaattgttgg ttgcgaacgg aattaacgtg caacctggtc acactttggc
    91261 tctctctatt gatgtggagc aacgtgaatt ggcacatcta atcgtgaaag aagcttatgc
    91321 cttgggtgcg catgaggtca tcgttcagtg gacagatgat gtgattaacc gtgagaaatt
    91381 cctccatgcc ccgatggagc gtttggacaa tgtgccagaa tacaagattg ctgagatgaa
    91441 ctatctcttg gagaataagg ctagccgtct tggagttcgt tcatctgatc caggtgcctt
    91501 gaacggagtg gacgctgaca agctttcagc ttctgctaaa gctatgggac ttgccatgaa
    91561 gcctatgcgt atcgcaactc aatctaacaa ggttagctgg actgtagcag ctgcagcagg
    91621 acttgagtgg gctaagaaag tcttcccaaa tgctgcgagc gacgaagaag cagttgattt
    91681 cctttgggac caaattttca aaacttgccg tgtctacgaa gcagatcctg ttaaggcctg
    91741 ggaagagcat gcagctatct tgaagagcaa ggccgatatg cttaataagg agcaattttc
    91801 agcccttcac tacacagcgc caggaacaga tttaacactt ggtttgccaa agaaccatgt
    91861 ttgggaatca gctggtgctg tcaatgcaca gggcgaagga ttcttgccaa atatgccgac
    91921 agaagaggtc ttcacagcac ctgacttccg tcgtgcagat ggttatgtca cttctacaaa
    91981 accgcttagc tacaacggaa atattattga aggcattaag gtgaccttta aggatggaca
    92041 aatcgtagat atcactgctg agaagggtga tcaggttatg aaagaccttg tctttgaaaa
    92101 tgcgggtgcg cgtgccttgg gtgaatgtgc cttggtacca gatccaagtc caatttctca
    92161 gtcaggcatt accttcttta acaccctttt cgatgaaaat gcgtcaaacc acttggctat
    92221 cggtgcagcc tatgcgacta gcgttgttga tggagcggag atgagcgaag aggagcttga
    92281 agctgcaggg cttaaccgtt cagatgttca cgtagacttt atgattggtt ctaaccaaat
    92341 ggatatcgat ggtattcgtg aggatggaac gcgggtacct cttttccgta atgggaattg
    92401 ggcaaattaa ggagataata tgttaggaag tatgttcgtt ggtctcctag tgggattttt
    92461 agcaggtgct atgaccaatc gtggagagcg aatgggatgt tttggaaaaa tgtttctcgg
    92521 ttggatcgga gcctttctag gtcacttgct ctttggaact tgggggccag ttttatcagg
    92581 aacagctatt atcccagctg ttttaggtgc catgattgtc ttagctattt tttggagacg
    92641 aggaagttaa tttcctaaat ctgatactca atgaaaatca aagagcaaac taggaaacta
    92701 gccgcaggtt gctcaaagca ctgctttgag gttgcagata gaactgacga agtcagtaac
    92761 atatatgcgg caaggcgacg ttgacgcggt ttgaagagat ttttgaagag tatgaaacga
    92821 aaaggaggtt ggtcattgta ctagcctcct tttgatatga tataatagtt ctatgagatt
    92881 agataaattt ttagttgcct gtgcggtggg aagtcggact gaggtcaaaa acttgctcaa
    92941 ggctgggcgc gtgactgtaa atggtaaaaa agaaaaatca gctaaattgc agattgatga
    93001 aaaaatagat gagattcgct ttgatgggca agtgttggag tatgaagagt ttgtctacta
    93061 catgatgaac aagcccaaag gagttatctc agcgactgag gatcccaagc acagaaccgt
    93121 tctggacttg ttggatgact tggcgcggag taaggaagtt tttccagtag gacgcttgga
    93181 tattgacacg catggtcttt tgctcttgac caataatggt cagctggctc atgttcttct
    93241 ttctcccaag cgtcatgtgg acaagactta tcaggctcag gtcaagggaa ttatgaccca
    93301 agaagatgtg gagacattta tcaagggcat tccgctcaaa gactttacct gtcagcccgc
    93361 tagactggag attctttcca cagatgcaga aaagaatcag agccaaatcc gtgtgaccat
    93421 tgcagaaggg aagtttcatc agatcaagcg tatggtgggc tactgtggca aggaagtggt
    93481 ggacttgcaa cgattgacta tgggaacttt agtattagat gagaacctgg aacgagggga
    93541 atggcgtcgc ttgaccaagg aagaattaga aattctccgc gctaatatta tttaatttag
    93601 tttaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgtacttgag
    93661 tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtataa attcgaaaaa
    93721 ggtgagggag aatgtcctta ccttttatgt tttttagttg cttcctttgt gaaaagagtt
    93781 ataatagact gtagaataaa aggaggaatc tatgaacgcg attcaagaat catttactga
    93841 taaactattt gccaactatg aagcaaatgt aaaataccaa gcgattgaaa atgctgccag
    93901 ccacaacgga atttttgcag ctctggaacg tcgccaaagc catgtagaca acacacctgt
    93961 tttctcattg gatttaacca aggacaaggt cactaaccag aaagcgtctg gtcgttgctg
    94021 gatgtttgcg gctctcaaca ccttccgcca caaactcatc tcgcaataca aattggagaa
    94081 ctttgagttg tcacaagccc acactttctt ctgggataag tatgagaaat caaactggtt
    94141 cttggagcaa gtcattgcga cttcagacca agaattgact agccgcaagg ttagcttctt
    94201 actccaaaca cctcaacaag atggtggtca atgggatatg gtcgtttccc tctttgaaaa
    94261 atacggtgtc gtgcctaagt cagtttatcc tgagtctgtt tcatctagca gcagtcgtga
    94321 gctaaatgcg atccttaata aattgcttcg tcaagatgct caaatcttgc gtgatttgct
    94381 tgtttctggt gcagatcaag tgactgttca aactaagaaa gaagacctct tgcaagaaat
    94441 ctttaacttt cttgctatgt cattaggact tccaccacgc aagtttgact ttgcttatcg
    94501 cgataaagat aacaactaca aaagtgaaaa aggaatcaca ccacaagagt tctacaagaa
    94561 atatgtcaat cttcctttag aagactacgt ttctgttatc aatgctccaa ctgctgataa
    94621 accttacgga aaatcttaca cagttgagat gttggggaat gtggttggta gccgtgcagt
    94681 tcgctacatc aacgttccaa tggagcgctt gaaagaattg gcgattgccc aaatgcaagc
    94741 aggtgagact gtttggtttg gttctgatgt cggccagctc agcaaccgta aggctggcat
    94801 ccttgcgaca gatgtttatg actttgaatc aagcatggac attaaactta ctcaagacaa
    94861 ggctggacgt ttggactata gtgaaagctt gatgacccac gccatggtct tgacaggtgt
    94921 tgacttggac gaaaatggta aatcaaccaa gtggaaggtt gaaaactcat ggggagacaa
    94981 ggtcggtaca gatggttact ttgttgcctc agacgcttgg atggacgaat acacatacca
    95041 aatcgttgtt cgtaaggaat tgctgacagc agaagaacaa gctgcctatg gagcagaacc
    95101 aatcgtactt gcaccatggg atccaatggg agccttggct gaataaaagc atagaaaaaa
    95161 ggaatcagat tttagaacct ggttcctttt tagttgcttg attacatgat gtgaagaaca
    95221 tgtgccacaa tacccactgc gaagagggca aggataatag tgattggaga tacttttttc
    95281 ttaagtaagt acatgcaaag taaagtaagg agtagtcctg ataatccagg aatcaacata
    95341 tccaagtttt gttggaaagt agtaactttt tcaggagttt gagacaatcc ttgtcctact
    95401 tgtgcgaatg cttcttggat acctttagac ccttctggca atttatccca atggatataa
    95461 gccttttcat ctagttgaac tttagaaaca tcgaaagcaa atttaatatt tacccagcgt
    95521 tgaacaagga cagcaagaat gaacatccca aggatagaag ctcctttagt gatatcttga
    95581 aggataccac cagacatatc tttagtgatt tctgatccag ccttgtatcc aatctcttga
    95641 acataccaca agaatgacat acgaatcaag ttccatgcaa caaagaagag gagtggaccc
    95701 aagatattgc cagtaagggc aagtgaagca ccgagagatc caaggattgg gcgtactgta
    95761 aaccagaata ctgggtcacc gataccagca agaggtccca tcataccgat tttaacccct
    95821 tggatagcag cgtcatcgat ttccacaccg ttagcacgtt cttcttcaag cgcaagagta
    95881 acccccatga ctggagcagc tacgtatgga tgagtgttga agaactcaag gtgacgctca
    95941 agagcagcga tttgatcttc ttttttagta tagagttttt tgatagctgg aatgagtgta
    96001 taagcccagc ccaagttttg catccgttca aagttccaag acccttgtaa gaaggttgaa
    96061 cgccaccaaa cttttttacg atctgattta gttaattgaa gtttttcagt catgatgttt
    96121 tcagtccttt cttatcttag tagtcttcta ggatatcgcc gattgggtcg ttagaagttg
    96181 cggctcctcc gccaccattt ccaccagttt tagaaaggtg aaggtagata agagcgatag
    96241 caacaccgat agcaccgaat ccgattagag taatatctga cacagcagcg agaacgaaac
    96301 caagagcgaa gaatggccat acttcacgag ttgccatcat gttgataacc atggcgtaac
    96361 caacggcaac gaccatacca ccaccgatag ccataccatc tttgagccag tctggcatgg
    96421 cactaaggat actttgtaca gtttcagttg gtaccataag gagaagagct gcaggaagcg
    96481 cgatacgaag tccttggaaa agtagcgcaa tgaaatgcgc acgctccaca gcgccgaagt
    96541 cacctttttt agcggcagca tctgcagtat gaaccaaacc aactgaaatt gtacgaacaa
    96601 tcattgtcaa gaaaagtcca gctacggcaa gagggatagc aaccgcttgg gcaacaccga
    96661 taccagtctt ggtaaagtca ccaccaagaa ccatgataat ggcagcagcg acagaagcaa
    96721 gtgcagcatc aggagcgata gcagcaccga tatttgacca accaagggca atcatttgaa
    96781 gcgatccacc gaggataatc cctgcttcca agtgacctgt tacaagccca ataagggtac
    96841 aggctacaag tggttggtga aattggaact ggtcgaggat gccttcaaga cctgcaaaga
    96901 aggctacaac gactactaaa accatagaaa taatagacat gtttaaaatc ctttcataaa
    96961 taatggctta tttgacattg gctttgttaa tcaagtcaaa caaatctttt ttagaatcat
    97021 ttggtacttt acggacatca aattcaacac ccaagtcacg cattttttca aatgtagcaa
    97081 catcttcttt gtccatagac aaaacggtat tgaccaatgt tttacctgtt gagtgagcca
    97141 tagaaccaac gttaagagtc ttgattggca cgccgccttc gatggcacga agggcatctt
    97201 gaggtgtttc aaacaagata agggcatgtg tttctccaaa acgtgggtct tttgaaatct
    97261 caatcagttt ttgaattgga accacgttag ccttgacatt acctggagct gcttgtttaa
    97321 tcaattcttt acgaaggtcg tctttagcca cgttatctga agcaacgatg atacgatttg
    97381 cttttgaatc tggagtccaa gcagttgcaa cctgaccgtg aagtagacgt gtgtcaagac
    97441 gggcaagatt gattttcaat ttaccgtctc cgataacagt tccttctggg atagcagttt
    97501 gggcaactgg agcagctgca gcgcttgcaa cttcttcaac tggatttagc tcttctggaa
    97561 gagctttgat gccatctttg gcttctttaa tgatattagc agcgactttt tctacacctg
    97621 cagcagcgtc catgaggcgc tctgtgtagg cttgaatcaa catcggtaag ttaagtcctg
    97681 tgatgatggc aaacttacgc tcaggatttt ctcccatcac gcgactagct tggttaaatg
    97741 gagaaccact ccaaaggtca gccaaaacta gaacctcatc ttctgcgtca aatgcagcaa
    97801 cagcgttatt aaacttagcg tatagatcat caggaccttc atttggcata aaggtcacaa
    97861 cttgaacctt ttcttgttca ccaaagatca tagatcctga ctgatgaata cccgcagcaa
    97921 attcgccgtg gctcgcaata atgattccga tactcattat tgtcattcct ccttttttgt
    97981 tttagttttc atttaagaaa actttaagac tgtttaagta taaaccgt
//
LOCUS       DAVKMU010000003        69254 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKMU010000003 DAVKMU010000000
VERSION     DAVKMU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 69254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.003"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..247
                     /locus_tag="VW343_000246"
     CDS             <1..247
                     /locus_tag="VW343_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9754871.1"
                     /translation="MATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATG
                     WFTVSGQWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
     gene            complement(726..875)
                     /gene="rpmG"
                     /locus_tag="VW343_000247"
     CDS             complement(726..875)
                     /gene="rpmG"
                     /locus_tag="VW343_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9754872.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(891..1073)
                     /gene="rpmF"
                     /locus_tag="VW343_000248"
     CDS             complement(891..1073)
                     /gene="rpmF"
                     /locus_tag="VW343_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9754873.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            complement(1225..1425)
                     /locus_tag="VW343_000249"
     CDS             complement(1225..1425)
                     /locus_tag="VW343_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9754874.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            complement(1428..2435)
                     /locus_tag="VW343_000250"
     CDS             complement(1428..2435)
                     /locus_tag="VW343_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9754875.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            2626..4656
                     /locus_tag="VW343_000251"
     CDS             2626..4656
                     /locus_tag="VW343_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9754876.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            4660..4944
                     /locus_tag="VW343_000252"
     CDS             4660..4944
                     /locus_tag="VW343_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9754877.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            4957..6303
                     /locus_tag="VW343_000253"
     CDS             4957..6303
                     /locus_tag="VW343_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9754878.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFITKPSAKEE"
     gene            6307..7164
                     /locus_tag="VW343_000254"
     CDS             6307..7164
                     /locus_tag="VW343_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9754879.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            7161..8093
                     /locus_tag="VW343_000255"
     CDS             7161..8093
                     /locus_tag="VW343_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9754880.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            8326..10029
                     /gene="ilvD"
                     /locus_tag="VW343_000256"
     CDS             8326..10029
                     /gene="ilvD"
                     /locus_tag="VW343_000256"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9754881.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(10190..10270)
                     /locus_tag="VW343_000257"
                     /pseudo
     CDS             complement(10190..10270)
                     /locus_tag="VW343_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10328..10669)
                     /locus_tag="VW343_000258"
     CDS             complement(10328..10669)
                     /locus_tag="VW343_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9754882.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            10793..11644
                     /gene="rgg"
                     /locus_tag="VW343_000259"
     CDS             10793..11644
                     /gene="rgg"
                     /locus_tag="VW343_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9754883.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            11837..13084
                     /locus_tag="VW343_000260"
     CDS             11837..13084
                     /locus_tag="VW343_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9754884.1"
                     /translation="MKLLFRNPAYRILILSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            13162..14451
                     /locus_tag="VW343_000261"
     CDS             13162..14451
                     /locus_tag="VW343_000261"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9754885.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKEA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVSYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            14442..14522
                     /locus_tag="VW343_000262"
                     /pseudo
     CDS             14442..14522
                     /locus_tag="VW343_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            14792..15106
                     /locus_tag="VW343_000263"
     CDS             14792..15106
                     /locus_tag="VW343_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754886.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            15116..15322
                     /locus_tag="VW343_000264"
     CDS             15116..15322
                     /locus_tag="VW343_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9754887.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            15322..15519
                     /locus_tag="VW343_000265"
     CDS             15322..15519
                     /locus_tag="VW343_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754888.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQGDQFVWLYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            15522..16256
                     /locus_tag="VW343_000266"
     CDS             15522..16256
                     /locus_tag="VW343_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9754889.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            16268..16909
                     /locus_tag="VW343_000267"
     CDS             16268..16909
                     /locus_tag="VW343_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9754890.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            16927..17157
                     /locus_tag="VW343_000268"
     CDS             16927..17157
                     /locus_tag="VW343_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754891.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            17509..19272
                     /gene="aspS"
                     /locus_tag="VW343_000269"
     CDS             17509..19272
                     /gene="aspS"
                     /locus_tag="VW343_000269"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9754892.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            19250..20191
                     /locus_tag="VW343_000270"
     CDS             19250..20191
                     /locus_tag="VW343_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9754893.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(20401..20481)
                     /locus_tag="VW343_000271"
                     /pseudo
     CDS             complement(20401..20481)
                     /locus_tag="VW343_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(20617..21603)
                     /locus_tag="VW343_000272"
     CDS             complement(20617..21603)
                     /locus_tag="VW343_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9754894.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(21613..22413)
                     /locus_tag="VW343_000273"
     CDS             complement(21613..22413)
                     /locus_tag="VW343_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9754895.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PGQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(22674..23516)
                     /locus_tag="VW343_000274"
     CDS             complement(22674..23516)
                     /locus_tag="VW343_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9754896.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(23518..24810)
                     /locus_tag="VW343_000275"
     CDS             complement(23518..24810)
                     /locus_tag="VW343_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9754897.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(24921..26192)
                     /locus_tag="VW343_000276"
     CDS             complement(24921..26192)
                     /locus_tag="VW343_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9754898.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            26742..28259
                     /gene="malQ"
                     /locus_tag="VW343_000277"
     CDS             26742..28259
                     /gene="malQ"
                     /locus_tag="VW343_000277"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9754899.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            28285..30543
                     /gene="glgP"
                     /locus_tag="VW343_000278"
     CDS             28285..30543
                     /gene="glgP"
                     /locus_tag="VW343_000278"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9754900.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(31588..32160)
                     /locus_tag="VW343_000279"
     CDS             complement(31588..32160)
                     /locus_tag="VW343_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754901.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTTIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(32783..33631)
                     /locus_tag="VW343_000280"
     CDS             complement(32783..33631)
                     /locus_tag="VW343_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9754902.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            33783..34061
                     /locus_tag="VW343_000281"
     CDS             33783..34061
                     /locus_tag="VW343_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754903.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            34063..36126
                     /locus_tag="VW343_000282"
     CDS             34063..36126
                     /locus_tag="VW343_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9754904.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(36204..37460)
                     /locus_tag="VW343_000283"
     CDS             complement(36204..37460)
                     /locus_tag="VW343_000283"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9754905.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            37602..40067
                     /locus_tag="VW343_000284"
     CDS             37602..40067
                     /locus_tag="VW343_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9754906.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            40132..41043
                     /locus_tag="VW343_000285"
     CDS             40132..41043
                     /locus_tag="VW343_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9754907.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            41273..41971
                     /gene="dapD"
                     /locus_tag="VW343_000286"
     CDS             41273..41971
                     /gene="dapD"
                     /locus_tag="VW343_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9754908.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            42039..43169
                     /locus_tag="VW343_000287"
     CDS             42039..43169
                     /locus_tag="VW343_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9754909.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            43181..43720
                     /locus_tag="VW343_000288"
     CDS             43181..43720
                     /locus_tag="VW343_000288"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9754910.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVVYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            43704..44381
                     /locus_tag="VW343_000289"
     CDS             43704..44381
                     /locus_tag="VW343_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9754911.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(44453..45352)
                     /gene="galU"
                     /locus_tag="VW343_000290"
     CDS             complement(44453..45352)
                     /gene="galU"
                     /locus_tag="VW343_000290"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9754912.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(45374..46390)
                     /locus_tag="VW343_000291"
     CDS             complement(45374..46390)
                     /locus_tag="VW343_000291"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9754913.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            46612..47015
                     /locus_tag="VW343_000292"
                     /pseudo
     CDS             46612..47015
                     /locus_tag="VW343_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(47130..47555)
                     /locus_tag="VW343_000293"
     CDS             complement(47130..47555)
                     /locus_tag="VW343_000293"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9754914.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(47623..48273)
                     /gene="phoU"
                     /locus_tag="VW343_000294"
     CDS             complement(47623..48273)
                     /gene="phoU"
                     /locus_tag="VW343_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9754915.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(48288..49040)
                     /gene="pstB"
                     /locus_tag="VW343_000295"
     CDS             complement(48288..49040)
                     /gene="pstB"
                     /locus_tag="VW343_000295"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9754916.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(49042..49857)
                     /gene="pstA"
                     /locus_tag="VW343_000296"
     CDS             complement(49042..49857)
                     /gene="pstA"
                     /locus_tag="VW343_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9754917.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(49850..50713)
                     /gene="pstC"
                     /locus_tag="VW343_000297"
     CDS             complement(49850..50713)
                     /gene="pstC"
                     /locus_tag="VW343_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9754918.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(50831..51706)
                     /locus_tag="VW343_000298"
     CDS             complement(50831..51706)
                     /locus_tag="VW343_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU9754919.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGKYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(51873..53204)
                     /locus_tag="VW343_000299"
     CDS             complement(51873..53204)
                     /locus_tag="VW343_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9754920.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMVLTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(53205..53912)
                     /locus_tag="VW343_000300"
     CDS             complement(53205..53912)
                     /locus_tag="VW343_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9754921.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(53996..54298)
                     /locus_tag="VW343_000301"
     CDS             complement(53996..54298)
                     /locus_tag="VW343_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9754922.1"
                     /translation="MTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(54285..54506)
                     /locus_tag="VW343_000302"
     CDS             complement(54285..54506)
                     /locus_tag="VW343_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9754923.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            54468..55026
                     /locus_tag="VW343_000303"
                     /pseudo
     CDS             54468..55026
                     /locus_tag="VW343_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55341..57032)
                     /locus_tag="VW343_000304"
     CDS             complement(55341..57032)
                     /locus_tag="VW343_000304"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9754924.1"
                     /translation="MNTKELIASELSSIIDSLDQEAVLKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            57168..57614
                     /gene="argR"
                     /locus_tag="VW343_000305"
     CDS             57168..57614
                     /gene="argR"
                     /locus_tag="VW343_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9754925.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            57665..60199
                     /gene="mutS"
                     /locus_tag="VW343_000306"
     CDS             57665..60199
                     /gene="mutS"
                     /locus_tag="VW343_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9754926.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDCEGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            60346..62040
                     /gene="patA"
                     /locus_tag="VW343_000307"
     CDS             60346..62040
                     /gene="patA"
                     /locus_tag="VW343_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9754927.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(62371..62640)
                     /locus_tag="VW343_000308"
                     /pseudo
     CDS             complement(62371..62640)
                     /locus_tag="VW343_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            62832..64598
                     /gene="patB"
                     /locus_tag="VW343_000309"
     CDS             62832..64598
                     /gene="patB"
                     /locus_tag="VW343_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9754928.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAHQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPIMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            64737..65426
                     /locus_tag="VW343_000310"
     CDS             64737..65426
                     /locus_tag="VW343_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9754929.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            65430..65609
                     /locus_tag="VW343_000311"
     CDS             65430..65609
                     /locus_tag="VW343_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754930.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            65596..66945
                     /locus_tag="VW343_000312"
     CDS             65596..66945
                     /locus_tag="VW343_000312"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9754931.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            67069..68529
                     /locus_tag="VW343_000313"
     CDS             67069..68529
                     /locus_tag="VW343_000313"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9754932.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            68543..68977
                     /locus_tag="VW343_000314"
     CDS             68543..68977
                     /locus_tag="VW343_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9754933.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
     gene            69098..69169
                     /locus_tag="VW343_000315"
     tRNA            69098..69169
                     /locus_tag="VW343_000315"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69131..69133,aa:Glu,seq:ttc)
BASE COUNT        20334 a        12866 c        14983 g        21071 t
ORIGIN      
        1 aatggctact ggttgggtta aagacaaagg cttatggtat tacttaaacg aatcaggttc
       61 aatggctact ggttgggtta aagacaaagg cttatggtat tacttaaatg aatcaggttc
      121 aatggctact ggttggttta cagtttctgg tcaatggtac tatacctata attcaggaga
      181 tttattagta aacacgacta cacccgatgg ctatcgagtc aatgctaacg gtgagtgggt
      241 aggataggta tccattagct aaatctaatc attctgaact aaaaaatctt cttacatatg
      301 taggaagatt ttttggattc tcaaaactag tttaaaagac tacgctattt aaaccaattt
      361 taagaatgga tatataagga tattcagaaa tctagttggt caacttttgt attttgaata
      421 taccttagat ggtgacgatg tcttggtata tctggtactc acttacatat tctaacttat
      481 tttaactaag atacaactgt agttggtgaa tttgctacaa atagtgtaga ggcgttattt
      541 gaaaaatata caaaaaagga accccaactt acatttgagt tcctcttgct tttaattgtc
      601 cgaatccgta ttcttatttc aataatgtag tcattcgaat gatttccctt gcaatatagt
      661 gaaattaaca aaaagaaaaa acgctgatca agcgtttttc ttctgtattg attcgtattg
      721 aatgcttact tcacttctgt aaacacaacg tgtttgcgaa gttttggtga gtatttcttc
      781 aattgaaggc ggtctggagt gttacgtttg tttttagaag taaggtacaa gcgttcacca
      841 gattctttgt gttcaagtgt aatatttacg cgcatagtat ctcccttcta ttattcagct
      901 gatgcagctt tagcgatttt acgtcctttg tagtatcctt taagtgatac acggtgagaa
      961 cgtgagtaat ctccagtagt ttcgtcaaag tttacagatg gagctgttac tttgtagtgt
     1021 gtacgacgtt tgtttttctt cgcttttgaa gtgcgacgtg caggtactgc cattttgatt
     1081 tctcctttag gtatttatat tcgattcaat ctactgattt tcatcaacca tactaggata
     1141 acacattttt tttgtaaagt aaagttactt gacaaaaaaa gatgaaaaaa ttctcctatc
     1201 cgacacggca gacaggagaa ggtgttaaat atcaatctca aatggttcgt caatggtttc
     1261 tgatacgtat tttccgtctt ttttccgttg cttgacacac tctgtgagga gatattctat
     1321 ttgcccattg actgaacgaa agtcgtcttc tgcccatgat gcgagtgcag cgtataactt
     1381 tgttgagagt cgaaggggga tctgcttttt tttagcttca gccatcttta gtaaagactt
     1441 cctgtgttga caattggttg tgcatcatga ttgccacaaa gaacgacaag gagatttgaa
     1501 accatggcag cttttcgttc ttcgtcaagt tctaccaatt ccccttcatt gagccgttct
     1561 agtgccattt caaccattcc tacagcacca tctacaatca tcttacgtgc atcaataatg
     1621 gcagatgctt gttggcgttg aagcataacg gcagcaattt ctggagcata agctaggtaa
     1681 gtgatacgtg cttcaaggat ttccaagcca gcatcctcaa cacgactttg gatttcttca
     1741 cgaatacggt tagcaacaat ttcgctagag ccacggagac taccttcatc tgcttgcccg
     1801 tcacccgtag tatccacatt aggagacaca tcgtaaggat agatgcggac aatattacgg
     1861 agggcgctat cacattgcaa tgaaagatat tctttgtagt tatcaacgtt gaagactgcc
     1921 ttagcggtat cgacaactct ccaagttacc gcgataccga tttctacagg gtttcctaag
     1981 caatcattga ttttttgacg agaattgctc aaggtcatga ctttgaggga aatctgtttc
     2041 ttgccaattt caagatttac atcattgtca tttgatgatt tagctcctaa aaaaggagat
     2101 tttgtgctaa catcaccact ttgtccaagt cgagtgtggt ttgcagggtt gactgctacg
     2161 ctgaagggat tgacaaagta aaaaccaggt tctttgatgg tacctgtata attaccaaag
     2221 agtgtcagaa ccagagcttc ctgaggtttg acaactttta aaccagcatg agctagacct
     2281 gcaattacga ttagtaaagg tccgataata attccaaaaa tgttttccga accaacaccc
     2341 attataaata taaagatacc aagtacgatt gtcaattcaa tgagaatcaa tgcgaataca
     2401 ccatttggtt ttgaattgat gagtttttca gtcataagaa tgacctccta ttatttgata
     2461 tctaaatgat atcactttgt ttttaaaagt caatagaaag ttgaaaataa tcactaaaaa
     2521 gtttcagccc aataatttct tttaaaaggt gagattatat tgtgctcaaa tactctttta
     2581 ttcttataat ttttacacta taattgtagt tagaaaggaa gagaaatgtt tgattataga
     2641 gcactagtta ttttgatgac tttgatggat cattgtgagc tatcattata tgaattatct
     2701 gttaaggtga gcttacctat aaaggaagtc aaagaaggaa tagattattt agtgccttat
     2761 ctagctaata aagggatagt gctggataaa aaacaaggtc gctatagttt atcaaatcgt
     2821 acgaagcagt ctttgacaga tattattaag tcggatgaat tggttttacc aaagtcgact
     2881 cgcttagctt taatctatct ttacactttt tgccgattag attttatatc gaataatcat
     2941 taccaagact ttttgaaagt aagtaagaat acaaccttat ctgatattca atcattaaga
     3001 aagattatgt tagataatga tttggagcta gggtacagta gagcaaaagg ttatacttta
     3061 cacggttcag agtggaataa gcaccgctta gcttttcaaa tggttagtga gttgctggaa
     3121 tcctccatag ggatttgggg tttggattat gttttatcca gttgggggta ttcattaact
     3181 tatgatttga taaatcaggt agttaaagat tattatgaga agctacagct agtacctatt
     3241 gttaatcaat taaaagtttg ccttttcggt ttagtattca ttctgtgtag gtatcaaagg
     3301 gatgttgaaa gagtatgtct ttcagagaca ttagtatctc ctgttattca agatataacg
     3361 actattttat tggatacagt ggttgatttg ggaattatag atacggtgtt ttctgaggat
     3421 gattatcgct atatcaccgt tttattatca agttgctttg aaggtgaagt agatgttgct
     3481 ccggtttact ttaatcaatt aacagaggct attataagta gaatggaaga tatttctttg
     3541 ttacatttta aacaaaggga aggattgaga gaaaatcttc gccgccacct tataccggct
     3601 tactatagat taaagttcgg cctacctagt tcaaatgaat atgtattgca tgttaaagaa
     3661 cattatcctg atttatttga attagtcaaa gattctttga tgcccttgat ggacgctatt
     3721 gacaatccca tacctgatag tgaaacagca tattttgtta tccatttcgg aggttattta
     3781 aagaaagcag atactttgcc tcaaaaatgc tataaagcag caatcatttg tcctaatggt
     3841 gttagttctt cattgatgtt aaaagagaat ctattagcat tatttcctca aattgagttt
     3901 ataggaacct caaagattga tgatttacag gtgaaagcta gtagtgacta tgatatggtt
     3961 ttttctacca taaaggtgga gacagaaaag ccaaattatc tagtttcggt tatgatgact
     4021 gaagagcaag caatacaact agtagaacta gtgttaaaag attttccgaa tttagagtat
     4081 ggggattttg agattgaaca aatcctgaat attgtcaaaa gatatggaat tattacacaa
     4141 gaattggaat taagattggc gttaaagaat tatctttacc aaaaaaatga tagaaaggaa
     4201 attgtaccag tgctagaaca actcattacc aaagaaacct atcaggttag ttcgcaaaaa
     4261 ttaggatgga aggaggcgat tcgtttagca gctaaaccgc ttttggatca agataagata
     4321 actgagaact accctgaggc aatgattcaa aaagtagaag agtttgggcc ttttattaat
     4381 ttagggaagg gagtagcaat tcctcacgct cggccagatg aaggtgtgaa tgaaatagga
     4441 atgtcaatgt tagttttgga agaaccgatt tatttattgg ataatccaga acaagaggta
     4501 agattgttga tttgtattgc agccattgat aatgagagtc atttaaaggc tttgtcacat
     4561 ttaacaacaa ttttaagaga taaaaatcat gttcaaactt taatatcatc aaaaaactat
     4621 gatgacatta aaatgataat taaacaggag gattagataa tgttaaaaat tggtacagct
     4681 tgtggttcag gattaggttc aagttttatg gtacagatga atattgaatc tgtattgagt
     4741 gatttgaatg tttcggatgt agaagttgaa cattatgatt taggtggagc agatccaaat
     4801 gcagctgata tttggattgt tggtcgtgat ctagctgatt cagctagtca tcttggagat
     4861 gttcgtatct taaatagtat tattgatatg gatgaactac gagaattaat tactaaactt
     4921 tgtgaagaaa aaggacttat ataggaggct agcgttatga tgaagttcat attggatatt
     4981 gttagtacac cagctatttt agtagcttta attgcaattt taggattagt tcttcagaag
     5041 aagaaattac ctgatattat taaaggtgga attaagacct ttgttggttt cttagttgta
     5101 tctggtggtg caggaattgt acaaaattct ttaaatccat ttggtaccat gtttgagcat
     5161 gcttttcatt tatctggcgt tgtgccgaat aatgaagcaa ttgtagctgt agctttaaca
     5221 acatatggct cagctactgc actgattatg tttgcaggca tggtgttcaa tatcttaatc
     5281 gctcgtttta ctcgatttaa atatattttt ttaacagggc accacactct atatatggca
     5341 tgtatgattg cggtcatttt atcagttgct ggctttacta gcttgcctct tatcttacta
     5401 ggaggattag cactcggtat tattatgagt atttccccag catttgttca aaaatatatg
     5461 gttcaattaa ctggaaatga caaggtggct ttaggtcatt tcagttcttt gggatattgg
     5521 ttgagtggtt ttactggtag ccttatcggt gacaaatcaa aatcaacaga ggacattaaa
     5581 tttccaaaga gtttagcttt tttacgtgat agtactgtta gtatcacttt atccatggca
     5641 gttatttaca ttattgtagc tatctttgca gggtcagaat atatagaaaa agaaatcagt
     5701 agtggtacaa gtggtctagt ttatgcttta caattagcag gtcaatttgc agcaggggta
     5761 tttgttattt tagcaggtgt tcgccttatt ttgggcgaaa ttgttccagc ctttaaaggt
     5821 atttcagagc gtcttgtacc taattcaaaa cctgctttgg attgtccgat tgtttatact
     5881 tatgcaccca atgcagttct aattggattt atctctagtt ttgttggtgg tttagtaagt
     5941 atggcaatta tgattgcttc aggaacggtt gttatcttac caggtgttgt gcctcatttc
     6001 ttctgtggag cgactgcagg tgtcattggg aatgcatctg gtggtgttcg tggagccact
     6061 attggagcat ttttacaagg tattttaatc agttttcttc cagtcttttt aatgccagtt
     6121 ttgggaggac ttggtttcca aggatcaact ttctcagatg cagattttgg tctatcagga
     6181 attattttag gaatgttaaa tcaatttggc tcacaagcag gcattgtgat tggtcttgtt
     6241 cttattctag cagttatgtt tggagtatcc tttattacaa agccatctgc aaaggaggaa
     6301 taagggatga ttttaagtaa aaatagggaa gatgagttaa gaaaatttgc gactaacatc
     6361 cgattaaata ctcttagaac attgaatcat cttggattcg gacattacgg aggaagtctg
     6421 tctatcgtag aagttttagc agtgctttat ggtgaaataa tgccaatgac tccagaaata
     6481 tttgcagcac gagatagaga ttatttcata ttatcaaaag gtcacggagg accagctcta
     6541 tacagtacac tctatttgaa tggtttcttt gacaaagaat tcttatattc tttaaataca
     6601 aatggaacca aattaccgtc tcatcctgat agaaatctaa cgccaggcat agatatgaca
     6661 acgggctctt taggacaagg aattagtgtt gcaactggac ttgcatatgg tcagagaata
     6721 agaaagagtc ccttttatac ttatgctatt gttggagatg gtgagttaaa tgagggacaa
     6781 tgttgggagg ctatacagtt tgcttctcat caacagttat ccaacttaat tgtatttgtt
     6841 gatgataaca aaaaacaatt agatggtttt acaaaggata tttgtaatcc aggtgatttc
     6901 gtagaaaaat tttcagcatt tggatttgaa tccattaggg tcaagggttc agatattaga
     6961 gaaatttatg aagggattgt ccaattaaaa cagtcaaata attcatcacc taagtgcatt
     7021 gtattagata ctattaaagg tcaaggggtt caagagctgg aagaaatgaa atccaaccat
     7081 catcttcgcc ctactgtaga ggagagacaa atgttaactt cagttgtaga aagattaagt
     7141 caggaattgg aggaaacaga atgatgagaa gcacgaaaga attacggcat gtatatagag
     7201 acttcctttt agaggctaat caaagttatt ctgatatagt agtcttagaa gccgatttgt
     7261 caagttcgat ggctactaat aatcttgaaa aggattttgg agaccgttat gtgaatgttg
     7321 ggattatgga agcagaaatg gtcggccttg cagcaggctt gtctattcag gggtttagac
     7381 cttatcttca tacatttggt ccttttgctt cacgaagagt atttgatcaa ttatttattt
     7441 ctcttggata cgcacaattg gatgccactg tgattggatc agatgcagga gtaactgcag
     7501 aaatgaatgg tgggacacat atgccatttg aagaaattgg attgttacgt ttaattccta
     7561 aatcgatcat ttttgaagca actgatgata tccaatttcg tgaaatattg aaccagacat
     7621 tagacttaaa aggactaaaa tatattcgaa caattagaaa agctccagtt cctgtgtatc
     7681 aaggtggaga agatttttct aagggctaca ttgagttaag gcatggtgaa gatcttgtaa
     7741 tcgttgcttc tggtataatg gttgctccaa gtattcgagt tgcagatgaa ctgtctaaat
     7801 taggttattc agtaagtgtg atagatttat ttagaatcaa accgatacca gaacagataa
     7861 aaacaatgtt aagtggaaaa actatattta ctgtagaaaa ccacaatcag ataggtggaa
     7921 ttggcagttc tttatgtgaa ttattctctg atgatggaat gacaaaaatt catcggatgg
     7981 gtgttcaaga aagattcggt caagtaggga aaatggatta tcttcttaat gagtatggtt
     8041 tgagtgaatc gaatataaaa gagaaggttc tagaaattta taatgcaaga tagatgttaa
     8101 atacacttat ctgaaagaat ttctgtaaaa attacagtac gttatttaaa gaaagcggat
     8161 taaaaagcat attctggtca tcagaaagtg cactaatccg ctttgttgtt tttaatttta
     8221 aatatttcac atgtgtatag aattttctga aaattcaaga tttttattct gttcttcatg
     8281 cgaaaattgt gctataatag taaaaactga aatgggaggg ataagatgac tgaattagat
     8341 aaacgtcacc gcagtagcat ttatgacagc atggttaaat cacctaaccg tgctatgctt
     8401 cgtgcgactg gtatgacaga taaggacttt gaaacaccga ttgtgggagt gatttcgact
     8461 tgggcggaaa atacaccatg taacattcac ttgcatgatt tcgggaaact ggctaaagaa
     8521 ggtgtcaaat ctgcaggcgc ttggcctgta cagtttggaa ccattaccgt agcagacggg
     8581 atcgctatgg gaacgcctgg tatgcgtttc tctctaacat ctcgtgacat catcgcggac
     8641 tccatcgagg cggctatgag tggtcacaac gtggatgcct ttgtcgctat cggtggctgt
     8701 gacaagaaca tgcctggatc tatgattgct attgctaata tggatatccc agctattttc
     8761 gcctatggtg gaactattgc accgggaaat cttgatggta aagatatcga cttggtttct
     8821 gtctttgaag gtatcggaaa atggaaccac ggtgacatga cagctgagga cgtgaaacgt
     8881 cttgaatgta atgcctgccc tggccctggt ggctgtggtg gtatgtatac tgctaatacc
     8941 atggcaactg ctatcgaagt tctagggatg agtttgccag ggtcatcctc tcacccagct
     9001 gaatcagctg ataagaaaga agatatcgaa gcagcaggac gtgctgttgt taagatgttg
     9061 gaacttggtc tcaaaccatc agatatcttg actcgtgaag cctttgaaga tgctattact
     9121 gtaacgatgg ctctcggtgg ttctacaaac gccactcttc acttgctcgc cattgcccat
     9181 gccgcaaatg ttgacttgtc acttgaggac ttcaatacga ttcaagaacg tgtgcctcac
     9241 ttggccgact tgaaaccatc tggtcagtat gtcttccaag acctctacga agtcggtggt
     9301 gtccctgcgg ttatgaagta cttgttggca aatggtttcc ttcacggaga tcgcatcaca
     9361 tgtactggta agactgtagc tgaaaacttg gctgactttg cagacttgac tccaggccaa
     9421 aaagttatca tgccacttga aaatccaaaa cgtgcggatg gtccgcttat catcttgaac
     9481 gggaaccttg ctcctgacgg tgcagttgcc aaggtatcag gtgttaaagt gcgtcgtcac
     9541 gttgggccag ctaaggtctt tgactcagaa gaagatgcga ttcaggccgt tctgacagat
     9601 gaaatcgttg atggcgatgt agtcgttgtt cgttttgttg gacctaaagg tggtcctggt
     9661 atgcctgaga tgctgtcact ttcttcaatg attgttggta aaggtcaggg agataaggtg
     9721 gccctcttga cggacggacg tttctctggt ggtacttatg gtctggttgt tggacatatc
     9781 gctcctgaag ctcaggatgg tggaccaatt gcctatctcc gtaccggcga tatcgttacg
     9841 gttgaccaag ataccaaaga aatttccatg gccgtatccg aagaagaact tgaaaaacgc
     9901 aaggcagaaa caaccttgcc accactttac agccgtggtg tcctcggtaa atatgcccac
     9961 atcgtatcat ctgcttcacg cggagccgtg acagacttct ggaatatgga caagtcaggt
    10021 aaaaaataaa ctcatactcg aaaatctctt caaatcacgt caacgtcgcc ttgccgtagg
    10081 tatggttact gacttcgtca gttctatcca caacctcaaa acagtgtttt gggctgactt
    10141 cgtcagttct atccacaacc tcaaaacagt gttttgagct gacttcgtca gttctatcca
    10201 taacttcaaa acagtgtttt gagcaacctg cggctagttt tctagtttgc cctttgattt
    10261 tcattgagta tcaaaaagca agccattttc ggcttgcttt tttcatcttt aaaagtgatt
    10321 ggcctgctta acgaggtggt agtccaaggg caatacggcc atagcgactg attcgtgtga
    10381 ttttccaagc aggcgaccag gtaacttcaa ccttgatatc tttgataccc tcgatttgtt
    10441 tcagacctgc cacaatttca ataggcaggc tttcggcgca atcacaggca gtatcggtga
    10501 aggtcatgac aatcttgcag agccccgttt catccagatt gatttcataa atcagaccta
    10561 gattgtaaac atccaattcc acatctgtat caaaaacttt ctctagtttt tcgataattt
    10621 ggtcttgcaa ggccaaagca cggtcattga ttttgatatc gtctctcata gcagtccctc
    10681 cacgtgataa atatcctcta ttttctcata attctgacaa tttggcaagt ttttgatgaa
    10741 ttttctgaaa aataggataa aatgaagaaa atacggaatc aaggggaagt ctatggagca
    10801 gattggaaaa gtctttagac aattacgaga gtcaagaaat atctcactga gacaagcaac
    10861 cggaggacaa ttttcgccgt ctatgttgtc ccgttttgaa acaggtcaga gtgagctttc
    10921 ggtggaaaag tttctatttg ctctggaaaa tatatctgcc agtgtggagg agatcctctt
    10981 tctggcgaga ggtttccagt atgatacaga ttctgagttg agaaaagaaa ttacagatgt
    11041 cttggaacca aagaatatag cacctcttga ggacttatat cgcagggagt atcaaaagca
    11101 tgcccattct cacaacaaac agaaacatat tctaaatgcc attattatca agtcttatat
    11161 gaagagcatg gatgaaaggg tagagttaac agcagaggaa ggaaaagtcc ttcatgatta
    11221 tttgttttct accgagatct ggggaatcta tgaactcaat ttattttcag tcagttctcc
    11281 atttttatct gtttctcttt ttactagata tgtacgagaa atggtacgta aatctgattt
    11341 tctaatggaa atgtctggca atcgaaacct ttttcatact atactattga atggcttttt
    11401 agctagtatt gagtgtgaag aatttaccaa tgcctattat tttaaacgtg ttatcgaaga
    11461 gcatttctac aaggaaaatg agacctattt ccgaattgtt tatctgtggg cagaaggtct
    11521 ccttgatagc aagcaaggta gagtcaagga aggccagaaa aagatggagg atgctgtccg
    11581 tatttttgag atgcttggct gtaataaatc tgccgaatac tatagaaata cgaccgaatg
    11641 ttgaatatct gcaaaccaag aaaaaatttg aagcgataga actattgccc tctctcaggt
    11701 cattacaaaa gttctatcgt ttctttttgc tcattttatt tgttgcatat attcaagagt
    11761 tctccctctc aaatttctaa gtgctatact atagtcatac ttcatataga ggttcgaaca
    11821 agaagggaga ttatctatga aactattgtt tagaaatcca gcttatagaa ttttgatttt
    11881 atcacgcttc ttcaatgctt ttggtgcttc gattttcaac ctggtcttta tcgtctatgc
    11941 atcgaccttg tcacaagcat cttttgctgt tgctatggcg aatattgtca tgattcttcc
    12001 gaccctcttt acagtttttg tagggattcg ggcagattac acgagggaca aggtcaaatg
    12061 gatggtctat agcggtttgt ttcaggcggt tctatttttt ctagcagccc tagttgttca
    12121 gcaagctagt ctctttgcct tttctagcct gtgtttgatc aatgtcatta gtgatatcat
    12181 cagtgatttt gcaggtggcc tgcgtatgcc tcttattaag gaaaaagtag ctgaagatga
    12241 tttgatggag gcttattctt tttcacagtt catcacctat atttcagcta ttggtggtca
    12301 agctttcgga gtctggctct tagctctatc ggtcaacaat ttttctctcg ttgcgggaat
    12361 caatgcctgc tttttcctag tatcagccac tatcctcttt ttaggaaaaa gcaaattgag
    12421 cctgtcaatg tcatctgctg atggtgaaaa cttaaaaaat gagaagcttt ctatcaaaga
    12481 ccagttccta acaatttacc gaaatttacg tctcgttttt cttaaaagtg ggcagaaaaa
    12541 ctttggtttt atgctctttg ctgtcttgct tattaattcc ttgggtggcg ctttgggtgg
    12601 aatttataat atcttctttt tgagccattc tctcttgaat ttttcttata cagaggcact
    12661 atttatcaat caattctgtg ttttagtagc aatcatcatc agtagcctta cgggcaatga
    12721 ttattttggg aagcagtcct tgcctagatt gatgatgtgg gcgaccgtag gacttagtct
    12781 agttggtttg gctaatttat tcaatcaagt cgtgcttggt ttgctatttc tcttctttac
    12841 cctgtatgtg tctggtaaag ttcaaccaaa gattagtgcc atgctcatga aaaatctagc
    12901 tccagaggtt ctagctcgta ctagtaattt tttaggtcta ttgtttacct tatccatacc
    12961 tgtgggaaca gcttgttttt cactagtagc cgtatggaat atacagttga cttggatgct
    13021 atttgttggt ctttccttgc tagctatttt tttgacaatt cttaatctca aaaatgatat
    13081 ctaaatccta atttttttct tactgtttta atccctttat ttgtggtaaa ataagactat
    13141 taagtttaaa gaggattccc tatgaaatta caaaaaccaa aaggaacgca ggatatttta
    13201 cctgctgaat ctgctaagtg gcagtacgtt gagggctttg cccgtgagat tttcaagcgc
    13261 tataactatg cagaagtgcg cacgcctatt tttgagcatt acgaggttat cagtcgctct
    13321 gtcggagata caacggatat cgtaaccaag gaaatgtacg atttttatga caagggtgac
    13381 cgtcatatta ccctccgtcc agaaggaact gcgcccgttg tccgttccta tgtggaaaat
    13441 aaactcttcg ccccagaagt gcaaaagcca agcaagttct actatatggg acctatgttc
    13501 cgttatgagc gtccacaggc agggcgcttg cgccaattcc accaaattgg tgttgagtgt
    13561 tttggctcta gcaatccagc taccgatgtg gaaacaatcg ctatggcagc ccattttttg
    13621 aaggaaatcg gtattcaagg tgtcaaattg cacctcaaca ctcttggaaa tcctgagagc
    13681 cgtgcagcct accgccaagc cttgattgac tatttgacac cgctcaagga gaccttgtct
    13741 aaggatagcc aacgtcgctt ggaggaaaat cctcttcgtg tcttggactc taaggaaaaa
    13801 gaagacaagg aggcagtaga gaatgcgccg tctatcttgg actttcttga tgaagaaagc
    13861 caagctcatt ttgatgctgt gcgtcagatg ttggaaaatc ttggagtaga ttacatcatc
    13921 gataccaata tggtgcgtgg tctggactac tacaaccaca ccattttcga gtttatcaca
    13981 gagattgagg gcaatgacct gaccgtctgt gcgggtggtc gttacgatgg tttggtttct
    14041 tattttggag gccctgaaac tgctggattt ggttttggac ttggtgtaga gcgcctgctt
    14101 ctcattcttg aaaagcaagg tgtgaccctc cctatcgaaa acgccctaga tgtctatatc
    14161 gcagtcttgg gcgaaggggc aaatatcaag gccttggaat tggtacaggc tcttcgccaa
    14221 caaggtttca aagcagagcg tgattacctc aaccgtaaac taaaagctca gttcaagtca
    14281 gccgatgtct ttgtggctaa gaccctcatc accctaggag agagcgaagt cgaaagcgga
    14341 caagtgacgg tcaagaacaa ccaaactcga gaagaagtgc aagtgtcact tgagacaatc
    14401 agccaaaact tctcagaaat ctttgaaaaa ctaggatttt atactcaata aaaatcaaag
    14461 agcaaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt gtggatagaa
    14521 ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagta
    14581 accataccta cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtattaat
    14641 gatagataaa gtggtcagga acttactcct gacctttttc ttttgcaaaa tgacaaaaat
    14701 cataaacttt ttgacaaata tacttgtcaa aaagaaaaag atatgttaca atgaattcaa
    14761 gttaagaaat aggaagcaga aaggagttat aatgtgggta ctaggattta tactatttat
    14821 gattttcttt tattctaata attctaaaaa aatcaagaaa ctagagaata aaatcaaaaa
    14881 acttgagcga aaagagaaag gaaacgcaga aatgtcgaga ttattacaag aaatgattgg
    14941 aaaggaacca attataacgg gagtgtatat tgggccagat aactgggaag ttgtggatgt
    15001 tgatgaggaa tgggtcaaac tacgtagtgt tgataatacg ggaaaagaaa aattcaagtt
    15061 gcaacgtatt gaagatatcc aaaccgttga atttgacgga gagtaggtgt gtaacgtgca
    15121 actaaaaaat cgtctaaaag agcttcgagc tcgcgatggt ctcaatcaaa ccgacctagc
    15181 caaactggca ggggtttcca gacagaccat tagcctacta gaacgggatg agtacacccc
    15241 atccattatc attgccctga aaatatctca aattttcaac gaaacagtcg aatcggtatt
    15301 tcgtttggag gaggacgagt gatgaacaag tataaagtga tttattatgt agtggtcata
    15361 gctttattag tcagcgtatt tctactgatt gggatggacc taagctggtt taatccctat
    15421 caaggcgacc aatttgtttg gctctacttt gctctcatcc cagtaattga atggattgaa
    15481 aagaaatcca aaaatctagc aagtgaaaaa ggagaatgaa tatgaaaaag aaacgtagat
    15541 tgttattttt gatgtctatt gtcttggggg ggttcttggg catgtttgta gggatgttta
    15601 aggcacgtgt cgaatcccac gaaattattt tagatgtaaa agccttgatg ccatggatat
    15661 cagctatttg tttactgata ggtttcatta gtatgttttt gactttcaat ttcttaaaga
    15721 aaagcagaaa atttcattcc ttgtatcaag aggaaatgga tgacgatctg aatgaaacct
    15781 attatgtgca aatgtatcgg aatcttgagt ttggaaccat tgcttttaat attacaggtg
    15841 tagcgattcc attggctatt tttatttcat taagtgaggt gattatattg catacaaacc
    15901 ctcaaacatt tttcctttct ttcttactct ttgtggtatt cttagtcgct caaaaatctc
    15961 tttttaaaac cattgcgatt gttcgtcagt ttgatttgga atttttcgct acaccaaagg
    16021 atgtcttgaa ctatataaat tcttatgatg aaggggagcg tcaggctaat ttggaacaga
    16081 gttttcgaat tttattccaa ttacaccaat atgtcttacc agccttatat atttttctta
    16141 ttatcatttc tttcttgaca ggagagattc agttactagc tttcttgctt gtaggagcca
    16201 tccatgttta tatcaatgtg atgcagttac ctatggtaaa acgttatttc aaataaagga
    16261 gttatctatg aaattactta aaaaccttgg ctggattctt ctagcccttc tatccttttt
    16321 atttatctat ggctttattc aggggctcgc gactgcgtcg cttgctttag gcgcttcacc
    16381 ctatgctgtt actctactct atgtggcctt ggctggagta tatgtgtacg gcatttacaa
    16441 atggtatcag aaggctcctg ttcatattga gaagagcggc tttaaccgat ttatttggct
    16501 tcctgccttg gtttggttct tatctctggt cgttcaattc ttcttgccag atgatccttc
    16561 agtaaatcag caaatagcga cagacttgac cttgtctcaa ccacttttct cattctttgc
    16621 tgtggttatt tttgctcctt tgacggaaga gattgttttt agagggatgt tagcacgcta
    16681 tctctttcct aagcaggaca atagtaaacg aaccctgatt tttcttctgg tatctagtct
    16741 tctatttgcc ttgattcatt ttccaggtga tgtgcaacaa ttttttgtct attttagcct
    16801 tggttttagt ttgggcttgg cttacattag cagaaaaggt ctggtctaca gtatttctct
    16861 tcacgctttg aataatttag tcggcttttt gatgattctc atgctataat agagtcagga
    16921 ggtcacatga aacgagtaat tttattagca gtgattcagg cagtcgttct atttttcatc
    16981 attggagcgc tagcttatgc cttcaaaggc gatttcttct ataactatct agcagttgtc
    17041 tttgctccta ttgcaggtgt actgcgtttt gggacggctt acataacgga aattgtcttg
    17101 cctcgaaagg cagccgaaat cgctgaaaag cgtaaagcag gcaaaaattc aaaataaaag
    17161 agtccggtga agttcatcgg atttttgtat tatactcaat gaaaatcaaa gagtaaacta
    17221 ggaagctagc cgcaggttgc tcaaaacact gttttgaggt tgtagatgaa actgacgaag
    17281 tcagctcaaa acatggtttt gaggttgtag atgaaactga cgaagtcagt aaccatacct
    17341 acggtaaggc gacgctgacg tggtttgaag agattttcga agagtattag cgctaatttt
    17401 tagggctttc acttgacttt taaagactgg caaacttttc tgatgatttt catgaagagc
    17461 ctgcgctttt atggtaaaat agtaacagaa taaaagagga gagaaataat gaaacgtagt
    17521 atgtatgctg gtcgtgttcg tgaggaacac atcggacaag aaataacctt gaaaggatgg
    17581 gttggccgtc gtcgtgacct tggtggtttg atctttatcg atcttcgtga ccgtgaagga
    17641 atcatgcagt tggttatcaa ccctgaaaaa gtatctgcag aggttatggc aacagctgaa
    17701 agccttcgta gcgaatttgt tattgaggtg actggtcagg tcgctgcgcg tgagcaagcc
    17761 aatgataagt tgccaactgg tgcggttgag ttaaacgtga cagctcttat tgtgcttaac
    17821 acagctaaga caacaccatt tgagattaag gatggcattg aggcaaatga cgatacacgt
    17881 ttgcgttacc gttaccttga ccttcgtcgt ccagaaatgt tggaaaatct taaacttcgt
    17941 gccaaggtga cccactctat ccgcaactat ttggatgagt tggagtttat cgacgtggag
    18001 acaccattcc tttctaagtc aacgcctgaa ggggcgcgtg attatttagt gccgtctcgt
    18061 gttaataagg ggcattttta cgctcttcct caaagtccac aaatcacgaa acagctcttg
    18121 atgaatgctg gttttgaccg ttactaccaa atcgttaaat gtttccgtga cgaggacttg
    18181 cgtggagacc gccagcctga atttactcag gtcgacttgg aaacgtcctt ccttactgag
    18241 aaagaaatcc aagatattac agaaggcttg atcgcgcgcg tgatgaagga aacaaaaggc
    18301 atcgaagtaa cgctaccgtt ccctcgtatg aaatacgatg atgctatggc tctttacggt
    18361 tctgacaagc cagatactcg ttttgacatg ttgcttcagg acttgacaga agtggtcaaa
    18421 ggtgtagact ttaaagtctt ttcagaagca cctgctgtaa aagcgattgt ggtcaaagga
    18481 gctgcggaca actattcacg taaagacatc gacaagatga cggaagtagc caaacagtat
    18541 ggtgccaaag gtcttgcttg ggtcaaggtg gttgatggag aattaaacgg accagttgcc
    18601 aagttcttga ctggtatcca agaagaattg acaacagcgc ttgctcttga agataaggac
    18661 ttggttctct ttgtggcgga tacgcttgaa gtggctaatg caacactggg tgcccttcgt
    18721 ggacgtattg ctaaagagct tggcttgatt gataatgata agttcaactt cctttgggtg
    18781 gttgactggc cgatgtttga atggtctgaa gaagaaggcc gctacatgag cgcccaccat
    18841 ccattcacac ttccacagga agagactgct cacgaattag aaggtgattt ggctaaggtt
    18901 cgtgccattg cttacgatat cgtcttgaac ggttatgagc ttggtggtgg tagccttcgt
    18961 atcaaccaaa aagaccttca agaacgcatg ttcaaggctc ttggtttctc aactgaagaa
    19021 gccaatgacc agtttggttt ccttcttgaa gccatggact atggtttccc accacacggt
    19081 ggtttggcta tcgggcttga ccgttttgtc atgttgcttg ctggagaaga aaatatccgt
    19141 gaagtcattg cctttcctaa gaacaacaag gcaactgacc caatgacaca agctccatca
    19201 acagtcgctc tcaaacaact agaggaactc agcttacaag tagaagaaga tgaaacaaac
    19261 aaaacgaatt aagcggtggc gctattatct gcgccgcttt gcttatcaga taaaaatttt
    19321 acgtgtctta caaagtatct ctcgagaaaa gtatgatgag aagatttcgg cctctctggt
    19381 ctatggtttt ttatcagcag tagcagttaa tttctttttc caaccagggc atgtgtattc
    19441 gagtggtgca acaggtctgg cacagattat ctctgccttg agtaatcact ggtttggttt
    19501 tcatattccg atttcgctaa gcttctacgc cattaacttc cctttgatgg tcttagcttg
    19561 gtatcagatt ggccataagt tcaccgtctt tacctttatc acggtatcta tgagttcctt
    19621 ctttatccag tttgtccctg tggcaacctt gacggaggat cccattatca attccctttt
    19681 tgggggtgtt gttatgggtt tggggattgg ttttgctctt cgaaacaata tctccagtgg
    19741 tgggacggat atcgtcagcc tgactattcg caagaaaacg ggtaagaatg tcggtagtat
    19801 ttctttcttg gtaaatggaa ctatcatgct gatagcaggt ttgacctttg gttggaaata
    19861 cgctctttat tctatgatta ccatctttgt ctctagccgt gtgacagacg cagtctttac
    19921 taagcaaaag cgtatgcagg ccatgattgt gacaaatcat ccagagaagg taattgaaaa
    19981 aatccataaa aaattgcacc gcggagcaac catgatccac gatgcagaag gaacctataa
    20041 tcacgagaga aaggcagttt taatcactgt cattacacgt gcagagttta atgaatttaa
    20101 acagattatg acacaagtgg atccaagctc ctttgtctct gtctcggaaa acgttcatat
    20161 tctaggaaga tttgttgaga tagataatta gtgattaata cttttcgaaa atctcttcaa
    20221 accacgtcag cttcaccttg ccgtatatat gttactgact tcgtcagttc tatctgcaac
    20281 ctcaaaacca tgttttgagc tgacttcgtc agttctatct gcaacctcaa aaccatgttt
    20341 tgagctgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc tgacttcgtc
    20401 agttctatcc acaacctcaa aacagtgttt tgagcaacct gcggctagct tcctagtttg
    20461 cactttgatt ttcattgagt ataaaaagat cttgggaaaa gactcaattt cagtagaaaa
    20521 ggaagtaaat cttcccacaa taaaacgcat agtatcaagt tttttcaata cctgatagta
    20581 tgcgtttttg cttttaacga ctctttgccc agtctcttat ttttcgataa ttttgtgggc
    20641 gatcaattga cggtaacaaa tttgtttatt gtttttatta tcattaataa tctggagaat
    20701 agtttcaagg gaaacacgac caagctctaa actattgata tcgacatagg ctgccaagtt
    20761 gagcttggga ttaaccgagt caaagctgag aacagggaca tccagctggt gtttggcaat
    20821 atagttacaa acaccttcag ctaggaggct atcggttgtg atgatagcat caatttgtgg
    20881 atcgtgcttg aataatcgct tgctaaattt atagcccttt tcttccagaa actcgtcggc
    20941 aaagtagatg cgattgttgt cagtggtaag tttgtaatgt ttaagcgcct gttcatagcc
    21001 cgttaaacgg tctttggtca cgaagagctt tttacttcct ccgataaagg caatgcgttt
    21061 gcagcctttt ttgatgaaat attcagtcgc atcaaaacca gcttgaacat tgtcgttgtc
    21121 gacaagtggg atgaaaggag atagagattt acctaagata aggaagggga actgttcttc
    21181 tgcgacgagt tttacgagag ggtcttcttc ttgggcatag agaaagatta gcccatctac
    21241 acgcttgccg tagaccattt gtgaaatagc gttgagacgc tccttctcat ctttccctgt
    21301 tgctatttga atggcatagt ggttttcaga tgcgacttga gagatgccac gtagaaccga
    21361 tggaaagaaa ggattctggt agaaggcgtc tgagtcatca ggaagaacta atccgataac
    21421 ctgagtatag ctgcttacca agctacgagc gttaaggttt gggtggtagt tgagttcctt
    21481 catagctttg cgaacgcgtt tttttgtttc gtcgctaatg gttgatttat tttgaataac
    21541 acgggttacg gttgaaggcg aaacaccagc agccttggcc acgtctttaa tcgtaacggg
    21601 cataaaaatc tcctatttat ggtaatttgg atcacggaaa tgtgttttat aaaagatagt
    21661 gaccagatac agaacaaaaa gaatattttg tacagtaatc agggttgtca tattttggcc
    21721 aaataatccc aaaataagtg taatcagagt cggcaatcct aaacagttca agataaagtg
    21781 atagcactct ttaaaggttc taaatgaaaa gaggcgtgat ttcttagtga tataaaggag
    21841 aagactagct cctagagaga cgataaagaa attcaaacca aagaggaagc tcgcaccgag
    21901 aactaggaag aggctgatat agacacgatt ttgttggtac cagtctttag aaattgcttg
    21961 ggtcaagctg tctttgcttt tgaaactctc agtctgaatg gctcggtaag agatgcgggt
    22021 cagttccttg ctttccttgc tgatgactag ttcatttgta tcgaaatgca gttgcaagtc
    22081 cttaggtaat tccttgattt gacctggacc aatcacaaca gaaggggctt gactagctgt
    22141 tccagtataa gttaatgtgc catcgacaat tgtagcatgt tcagagagat cctggacaac
    22201 ctcatttgtc agaggttcat agacattatc gataaaagtt tctagcggat aggtctcctg
    22261 ggagctgttt tggatagcaa tgggtaccat agacaagctg ataaggaaga tactggtaaa
    22321 gagaagttga aaccagttga gctcgaaacg tttggacagg ggcttacgaa acccccaaat
    22381 acttgaaaaa taggaaaatg gatatggaag cataggtatc tttctaaaag gtgttttcta
    22441 tatgagtatt attatataga gaaatgcgct ttatttcaag tctagggggt aaattgcttg
    22501 ctacaaggat attatttaca gtgacaatcg ctaatagtca atgaaaatca aagtgcaaac
    22561 caggaagcta gctgcaggtt gctcaaagca caactttgag gttggagata aagctgacgt
    22621 ggcttgaaga gattttcgaa gagtataaaa tgaaaaaggg tggcggggat aaattatccc
    22681 ttgtcgccac cacttgtaag tcctgaaaca aagttctttt gtaggaagaa gaagagaata
    22741 cagattggaa gggcgatgag gatagcacct gctgagaagt aggcaatctt caagtttttc
    22801 gcattgttaa cgaaggtttg gagacctacg gcaacagtaa agtattcttt ctcacgaagc
    22861 aagaaactag agaggatgta gtccccgaaa ggtcccatga aggcccagag agcttgtacg
    22921 gcaaccattg ggcgaacaag tggtagaaca atttgccaga agcggcggaa gtgtcctgca
    22981 ccgtctagtt ttgcagattc gtctaaagac attggcactg tatcgaagta gcctttcatg
    23041 agccaagcat tcatcgggat accaccacca acgtagagga agatgaggaa ccagttgtgg
    23101 ttaagggcgt tcaacataag cgccataacg aagaaggctg tcaaagcggc catagttggc
    23161 accatttgga tgatcaagaa gaagaccaaa ctttgtttac gagccaagaa gttgtaacgg
    23221 ctgtaagcat aaccagcaag tacgatgata cttgtttgaa cagccatggt aattaaggcg
    23281 ataatcaaag tgttgaggta ccaagtaccg tacaaggttt cagtgaagag gcctttaaag
    23341 ttatcaaaat tgaggtcgat attagtatct agtttaaagg ctgagacgtt acctgcttta
    23401 aaggctgaca tgatggtaat caacagtgga tagataatta caattgatag accaatcagg
    23461 taaaggtaag taaggctttg agtcagtcta cgtttgagtt taattgagtt attcatctta
    23521 gacgtcctcc atatcaaatg cgtgtagttt cttgaatgcg atcatagaga ttgagatgac
    23581 aatgatagag ataatcaagg taacagctgc cgccattgag tattgaggag atgtacctgt
    23641 tgtcaaacgg tagatccatg agatcaagat atcggttgaa ccagctccac ctccgacact
    23701 accaggtcct ccaccattga agaggtacat gatagagaag ttgttaaagt tgaaggtgta
    23761 ttggctaatc aaagtaggtg ccgcaacagc caaaatcatt gggaaagtga tgttgcggaa
    23821 tttttgccaa gcgttggcac cgtcaatata agctgcttcg taaaggtcgt taggaataga
    23881 ttgcaagata cccaaagtca aaacgtagat gtatgggaat ccgagccaac cttgcatcat
    23941 aatcaaggca atcttagtcc aagttgggtc tgttttccaa ggaataagag ctccatcaag
    24001 gaaaggaagg aatttagcca agattggcaa tacttgagtg ttgatagcac cgacactatc
    24061 gttaaacatg tttgagaatg tcaagatagt gatgaaggct gggacagccc aaggaagaag
    24121 gaaaataaca ccaaagatac gttttccttt gataaatggt tggttggcaa tgatagctgt
    24181 gaagatacca attacgattt gtaaagtaga agctgccaaa gcccaaatga tagtccaaga
    24241 aagaacagaa ccaaaggcag aacggaaggt actcaagctc caaatgtttg taaagttggt
    24301 caaaccaacc cagtccaaca acttgtttgg tggcaagtgt tggaagtcgt agttggtaaa
    24361 ggcgatcatc aaggttacga taactgggaa gataatcgcg aaggtcatgg caacataaga
    24421 tggaatgatc aagaggtaag ggaagccatt ttcataaatc cctttgatca tgtctttgag
    24481 tgtgcgtgga actggaattc cattgttaat gcgttttgca atcgtatgtg catctttgat
    24541 atttgagaaa taaaagagta cataaacgat tacaaagatt agatggaagg caccacgaat
    24601 cagcataaag agggaattat cacgacctgg tttgtcacca agagtgatga ggttgctcaa
    24661 ttcaggggtt gcaagtgcta ggaagtaaag gacaaatacg atggttacac caaggaagat
    24721 aaaacctttg gctttttgtt tattgtaaat ctgtcctaac ccaggaatga tagacagcag
    24781 ggctgcttta ctaggttgtt gcttttccat attaactcct ttcataggat actacttttt
    24841 tataagaaag ctaaattatt tgtgtctcta ttgataaatt caaaggggga tttgatttcc
    24901 accccccttg aacaaatttt ttattcacca aatttttgtt tgattgtttc tttgatcaat
    24961 gttacagcat cgttagcagc tgttttagca tctttttgac cacttacagc atcaaagagc
    25021 atatttttcg ctggatccca aactgcagac atttgagaga tgtttggcag tggttgagtg
    25081 ttcttgaact gtttgataac agctgttgtc aactcatcgt ttttaccttc agcgtatgaa
    25141 cgagcctcag tattagctgg gatttcgtta gtcttatcat ataatacttt ttgttgttca
    25201 gttgcaacaa ggaagtctac aaatttttga gaagcttcaa ggttcttaac ggcttgagga
    25261 atgacccaag ctttaccacc accgaatgca gcatattctt ttccatttgg aagagttggg
    25321 atagttgcaa ctccgtagtt tactttagca tctttaaagg cttgagcttt ccaaggtccg
    25381 tcgatgatag cagctgtttt accttcttgg aattgagttt ggattaagtt tccagcacct
    25441 tctgtatctt gcataccttt aggccatttt tcgtaccaag atttagcgta gttgatacct
    25501 gcgatagaac cgtcgtttgc aagaccgatg tctttagcgt ctttaccgtt ttggccaaag
    25561 acgtaagcac cgttaccggc aagaagtcca tatgtatagt agaagtttgt ccagtcagct
    25621 aggaaggcag tagttttacc atcttcacca gcgaatgcgt atttgctatc tttagcaagg
    25681 ttttccaagt cagcaaatgt ttttggagca tctttcacca agtctttgtt gtagtacata
    25741 acaagtgact cgataacggc aggagcaccg taaactttac cattagcagc tgttacaaga
    25801 gatttagttg tgtcgtctgt tttagcacca tcgctcaatt tcacttctga aagttgtccg
    25861 tcagaaccaa ggctacctac acggtcgtat ggagccatca taacatcagg gacattacca
    25921 gattggttgt caagagaaag tttatcaaga cctcctagag catcaccagt tttaagagtg
    25981 acttttactc cagcttcttt ttcataagct ttagcaacct cttcaatata gctcttatat
    26041 ccctcgtcta catatacagt gagttctttg acttcagatg aaccagaatc agcaggctta
    26101 tcagcagttt tgcttccgca agctaccaaa agcaagctag caagtgtagc agttccaagc
    26161 accgcagtgc tcttcataaa tttagatgac atagtgtatt cctcccaaag aatagcaagt
    26221 tttattgata aggaaacgca aacgttttcc tttatgagct tagtatagca caaatagaaa
    26281 acggttgcaa gtattttttg taaaaatttt aaaaaatttt taagcttgat ttgatagcat
    26341 aattttgcat tttaatagaa gaaaagtatg aaatagataa gaatacagta tttttaataa
    26401 aaatatatgg aatctgtcta aagagggaaa tcaggagggg ctacctccct gtttctaatc
    26461 cctatctatt ttctatagac gtttgtgctt tgttgcatat aaattcaagc tccctaagga
    26521 ggttttgtac tttgtagact gtctcgtgag agtcaaactt taggattaga tagttttaag
    26581 ttatctttgc taaaatgagt tagtctcctt aaaaataatc aaaaaagttt tttaaaaacg
    26641 cttgcaatta tgcttgaaaa ggagtatact tataagtaac gcaaacgttt gcgtctgcaa
    26701 aatacgcaac gttccattat tttaacacac gaggtgctat tatgaaaaaa cgtcaaagtg
    26761 gtgtgttgat gcacatctct tctcttccag gagcttacgg aatcggatca tttggtcaaa
    26821 gtgcttacga cttcgttgat ttcttggtcc gtacaaaaca acgttactgg caaatccttc
    26881 cattaggagc aactagttac ggggattctc cttaccaatc tttctcagcc ttcgcaggaa
    26941 acactcattt tatcgattta gatatcttgg tggagcaagg tttgttggaa gcaagtgacc
    27001 ttgaaggagt tgactttggt agcgatgcgt ctgaagttga ctatgctaaa atctactatg
    27061 cacgtcgtcc tcttttagaa aaagcggtga aacgtttctt tgaagtcgga gatgttaaag
    27121 attttgagaa atttgctcaa gacaaccaat catggcttga gctctttgct gagtatatgg
    27181 ctatcaaaga gtattttgac aatcttgctt ggactgaatg gccagatgca gatgctcgtg
    27241 ctcgtaaagc ttcagcactt gaaagctatc gtgagcaatt ggcagacaag ttggtttacc
    27301 accgtgtgac tcaatacttc ttcttccaac aatggttgaa attgaaagct tacgctaacg
    27361 acaaccacat cgaaatcgtt ggggacatgc caatctacgt agcggaagat tcaagtgata
    27421 tgtgggcaaa tccacatctc ttcaaaacag atgtcaatgg taaggctact tgtatcgcag
    27481 gatgcccacc agatgagttt tctgtaactg gtcagctttg gggtaatcca atctatgact
    27541 gggaagcaat ggacaaagac ggctacaaat ggtggattga acgcttgcgt gaaagcttca
    27601 aaatctacga tatcgttcgt atcgaccact tccgtggctt cgaatcttac tgggaaatcc
    27661 ctgctggttc cgatacagca gcacctggtg agtgggtgaa aggtccaggc tacaagcttt
    27721 ttgcagccgt taaggaagaa cttggtgagc taaacatcat cgcagaagac cttggcttca
    27781 tgacagatga agtgatcgaa ttgcgtgaac gtactggctt cccaggaatg aagattcttc
    27841 aatttgcctt caacccagaa gacgaaagca ttgatagccc acacttggca cctgctaact
    27901 cagttatgta cacaggaaca cacgataaca atacggttct tggttggtac cgtaatgaga
    27961 ttgatgatgc gactcgtgag tacatggctc gttacacgaa ccgtaaagaa tacgaaacag
    28021 tggtacacgc tatgcttcgt acagtatttt catcagttag ctttatggca attgcaacta
    28081 tgcaagattt actagaattg gatgaggcag ctcgtatgaa cttcccatct acccttggtg
    28141 gaaactggtc ttggcgtatg actgaagatc aattgacacc agctgtcgag gaaggtttgc
    28201 ttgacttgac aacaatttat cgccgaatta atgaaaattt ggtagattta aagaaataag
    28261 acaataatca ggagacaact aaacatgtta tcactacaag aatttgtaca aaatcgttac
    28321 aataaaacca ttgcagaatg tagcaatgaa gagctttacc ttgctcttct taactacagc
    28381 aagcttgcaa gcagccaaaa accagtcaac actggtaaga aaaaagttta ctacatctca
    28441 gctgagttct tgattggtaa actcttgtca aacaacttga ttaaccttgg tctttacgac
    28501 gatgttaaaa aagaacttgc agctgcaggt aaagacttga tcgaagttga agaagttgaa
    28561 ttggaaccat ctcttggtaa tggtggtttg ggacgtttgg ctgcctgctt tatcgactca
    28621 attgctactc ttggtttgaa tggtgacggt gttggtctta actaccactt tggtcttttc
    28681 caacaagttc ttaaaaacaa ccaacaagaa acaattccaa atgcatggtt gacagagcaa
    28741 aactggttgg ttcgctcaag ccgtagctac caagtaccat ttgcagactt tactttgaca
    28801 tcaactcttt acgatattga tgttactggt tatgaaacag cgactaaaaa ccgcttgcgt
    28861 ttgtttgact tggattcagt tgattcttct attattaaag atggtatcaa ctttgacaag
    28921 acagatatcg ctcgcaactt aactctcttc ctttacccag atgatagtga ccgtcaaggt
    28981 gaattgctcc gtatcttcca acaatacttc atggtttcaa acggtgcgca attgatcatc
    29041 gacgaagcaa tcgaaaaagg aagcaacttg catgaccttg ctgactacgc agttgtccaa
    29101 atcaacgata ctcacccatc aatggtgatt cctgaattga ttcgtctttt gactgcacgt
    29161 ggtatcgagc ttgacgaagc aatctcaatt gttcgtagca tgactgccta cactaaccac
    29221 acaatccttg ctgaggcgct tgaaaaatgg cctcttgaat tcttgcaaga agtggttcct
    29281 cacttggtac caatcatcga agaattggac cgtcgtgtga aggcagagta caaagatcca
    29341 gctgttcaaa tcatcgatga gagcggacgt gttcacatgg ctcacatgga tatccactac
    29401 ggatacagtg ttaacggggt tgcagcactt catactgaaa tcttgaaaaa ttctgagttg
    29461 aaagccttct acgaccttta cccagaaaag ttcaacaaca aaacaaacgg tatcactttc
    29521 cgtcgttggc ttatgcatgc taacccaaga ttgtctcact acttggatga gattcttgga
    29581 gatggttggc accatgaagc agatgagctt gaaaaacttt tgtcttatga agacaaagca
    29641 gctgtcaaag aaaaattgga aagcatcaag gctcacaaca aacgtaaatt ggctcgtcac
    29701 ttgaaagaac accaaggtgt ggaaatcaat ccaaattcta tctttgatat ccaaatcaaa
    29761 cgtcttcacg agtacaaacg ccaacaaatg aacgctttgt acgtgatcca caaatacctt
    29821 gacatcaaag ctggtaacat ccctgctcgt ccaatcacaa tcttctttgg tggtaaagca
    29881 gctccagcct acacaatcgc tcaagacatt atccatttaa tcctttgcat gtcagaagtt
    29941 attgctaacg atccagcagt agctccacac ttgcaagtag ttatggttga aaactacaac
    30001 gttactgcag caagtttcct tatcccagca tgtgatatct cagaacaaat ctcacttgct
    30061 tctaaagaag cttcaggtac tggtaacatg aaattcatgt tgaacggagc tttgacactt
    30121 ggtactatgg acggtgctaa cgtggaaatc gctgagttgg ttggagaaga aaacatctac
    30181 atcttcggtg aagattcaga aactgttatc gacctttacg caaaagcagc ttacaaatca
    30241 agcgaattct acgctcgtga agctatcaaa ccattggttg acttcatcgt tagtgatgca
    30301 gttcttgcag ctggaaacaa agagcgcttg gaacgtcttt acaatgaatt gatcaacaaa
    30361 gactggttca tgactcttct tgacttggaa gactacatca aagtcaaaga gcaaatgctt
    30421 gctgactacg aagaccgtga cgcatggttg gataaagtca tcgttaacat ttctaaagca
    30481 ggattcttct catctgaccg tacaatcgct cagtataacg aagacatctg gcacttgaac
    30541 taatactctt cgaaaatctc ttcaaaccac gtcagcttta tctgcaacct caaagcggtg
    30601 ctttgagcaa cctgcggcta gcttcctagt ttactctttg gttttcattg agtataagat
    30661 acaaatttat actaatacat tttgtaaaaa agcgagtttc gattgaaatt cgctttttta
    30721 aatgctgtag atttgggtca atcttgtcta aaaataggga aatcctagat acagtgaaga
    30781 ctttaaatgc tggtttttac tgtcctcagc cttatatttt ttcgtagttg attacctcac
    30841 acctcttgta ttcgcttaca taaagtatta taatataatt gtaggaaaga aggtgttttt
    30901 atgatataca cacttaaatt ggtgttgttt attacctttc ttgtaataag cttgttacct
    30961 gataagattt ttggaaaaaa taaaaaaatt tggaaaatag tttttgcaat attgacggca
    31021 gtggcagcat tgtcatttat gtactaagtt attttaagaa tgtagggaaa taaaccctac
    31081 attcttttta gttttttctg ttttctaaat tctatttatc caagcgcttc aacatttctt
    31141 gcttcttcgc ttcaagttct gcacgctttt cttcgatttc ggcatgtttt ttctcgagtt
    31201 cagaacaact tgcaccattg ctaaattctt ttcgccatca ggagataggg tgagtcgaca
    31261 tgtctattac tcacccaaag cagtcctaca aagcaggaat tttctgttac ttttttggaa
    31321 atagtaacgt ttatacagct ttgacacttc gtatcaaagc gccaaacaca ctccgaggga
    31381 tttacagaaa gcagaaaagg aatgatctgg tataagatca ttccttttct ctctttttct
    31441 ttaagtaatt atatacaatg tacgacgaag tcgtcattgc aatgctgatc caccacctaa
    31501 agggaacttt aaacaacatt gataagataa agaatataaa caacgaaaat acgttatacc
    31561 caattaattt tattgtatat ctcatgatta aaagttaatc cttccgttgt taggaatggc
    31621 atcattttta tcccataatt gtgctaaata agtccccggt gataataaat tcatagcgga
    31681 ttctaaagca acatcattta caaaccaact acctagatat ctagaaattg ctgaacgaat
    31741 agcacttttt gctgcatgtt ttccttttac tttaattaga tttgcaaggc ctgcagtagt
    31801 tcctcctaat gctaaagcta ttgtagtatc taatagagca cccatttgat taactgtaat
    31861 accttgccaa gctgctctaa atggagagta tgtaggtggg attgtataat cgccttgtaa
    31921 ttgtcggtta attacttctt tgatccattg ttgtgagacg tctggatgaa aagattggat
    31981 ttcgtttgca agtgtattga tttgttcttc tgttagagaa gtgacaggtt gaagttccat
    32041 atttgtttca atttgcgata cttgttcaga agcgtataca gctgaaacac ttggaatcgc
    32101 tgatacaatt aacacaattg acgtcaaaaa aaccgaaata aatttcatta atttgttcat
    32161 gagcttttct cctttttatt tgtatctgct tacattttat tatatactgt tattatagtc
    32221 aagaaaaata tgctattatg ttaaaaaaat atttttcaaa atataaatgg acggatttat
    32281 tttggatttt atttgttatt ttgacctgcc tctatattgg taaccatgat ttgtttactc
    32341 tcaatcatca agaattctct tttcgtggta gcttttgggg tttggtactg gccttatatc
    32401 acttattgtt cattgataag tttgtaattt cgaatcgaaa ataaagatta gaactatgat
    32461 tgactggcta cttttaatat tgattttgga ggaagatttt gtctctatta tttattattt
    32521 taaatttatc tattttgtac aatatctatt ctttgagacg ggagaaatcg aaatacttga
    32581 tttatacggc ctatatcata tttggggtaa atgtagtata tggtattcaa tggttattaa
    32641 gagaactgat ttcaactatt tccccttaat gtgtatggaa actataacaa aacgcataag
    32701 attaaggttt gtgtacctgt tcttatgcgt ttttgtaata ttgaagattt tcggtaagtt
    32761 ttataccttt gggaagtcta gtttagaatg ctttcccaac cagaatctct gcttcgatgg
    32821 taatctctgt cagttggctc cagtcaatct tgctctggtc aacatgaaag aggagaggag
    32881 tcatactgag tagggcttgg agttgttctg ctgtgatagt cttagtcaga gaggcagttt
    32941 gactagatag gatggtaaag tgttcctgga aatgctcctt gatatcttgg ttagaatact
    33001 ccttgtttgt cagctggtcc tgtactcttt gacggatttc tttgaggtga ttttcagttg
    33061 ggataacctt tatcaagata ccgtctttgg ataaaacgcg acgaaattct ccatagttgg
    33121 caggtgagaa gatatcaagc agaatatcca tgttagcgtc ttttatagga agtcgtgcca
    33181 agtcgccaac gaaccaattg actgcccagt tgggttcact tttagcagcg atttggactg
    33241 aatctttgga gatgtcaaag gcatagaaag ttttttcaga gtgactttct tgtagtttgc
    33301 gagaatagaa tccttcacca caaccgatat ccaaaattgt tgtggtagtt tttgagcttg
    33361 caagcaagtc agatacagca tctaagatag cttggtaaaa gccggcttct aggatttgtt
    33421 gacggttttg aaaattttcc ttgtcatagt tagcagattg cttgatttga ggtgctagat
    33481 tgacatagcc aaatttcgcc aagtcaaaag aatgacggtt gcagcacttg aaattagtct
    33541 ctaacagagt cagattttct tgacagatag gacaggcaaa ggcagtcgca gaagcaaaac
    33601 gctgaagttt gggcttgaga tttgtattca tagtttaagt gtatcaaaag aggcaaatga
    33661 aagcaacttt aggaataagt agatgggaga ttcagtagaa ataaaactaa ttctccaatt
    33721 tatagaatga aattgctttt atatctaaat tgctatataa taatgataag gaggaaataa
    33781 gtatgttaaa agaagtatta accgttgcaa aagttgcgaa aaaatcttca ttatttttgg
    33841 gtggtgtcgc atttggtacc cttggtttga aaatcttagc aagtaaggaa gctaaaaaag
    33901 gttattctaa agctttggct aaggcttaca agttgaaaga cgagctagat gcatctgttt
    33961 ctgttgtgaa gcaacatgga gacgatgtct tgcaagatgc caaatatttg tacgagcaag
    34021 agaaaaaaga agagcaatta gatagcctta taggtgaata atatgtcttt taaagtgcta
    34081 catagaggat accaacatat ccgactatca tcttcttttt cacttacctt ggatattcaa
    34141 gactatcttc gttccttggc gagagatgaa aaggggattg agtctatcca gttttacatg
    34201 gatcaacagc actttactct acgcataaaa gaaggctttt ctgtattaga taatgcagaa
    34261 gcctttttaa aaagaattga taaagggaaa gtttctgagt tgatgactct tcccattcgt
    34321 agagaagaga gtgcttattc tattgtttca ggtgcagcgg ttaagcgtgt actttttcgt
    34381 agttttgtgc cgtatcctat tcgctatata tggacttgtt atcaggcttt gggttatatt
    34441 agagaagcct atcaaacact agcgcgtaag gaactaacga tggaagtctt ggactgttcg
    34501 gctattttat tgtctttgtt tatgaaccaa tccaagacag ctagcaatat catgtttatg
    34561 cttgatttgg ggaatcattt agatcagtgg tccttgaaaa aaactgcaac agatttagaa
    34621 cagagtcttc ttgcaaaaga gagcgatgta ttcctagtac agggcgatac ggttgttagt
    34681 atcaagagtt ccgatgttca aataggagat gtcttgatct tatctcaagg aaatgaaatt
    34741 ctgtttgatg gacaagtagt ttcaggttta ggtatggtca acgaaagttc cttgacagga
    34801 gagagttttc cagttgaaaa aagagagtct gatttggttt gtgcaaatac agtattagaa
    34861 actggagagt tacgcattcg tgtaacagat aatcagatga acagccgtat tttacagctg
    34921 attaagttga tgaagaaatc tgaagaaaac aagaaaacga aacaacgcta tttcatcaag
    34981 atggcggata aagtcgttaa atataatttc ttgggggctg ggctgactta cctattgaca
    35041 ggttcttttt ctaaggctat ttctttccta ttggtcgatt tctcctgcgc tttgaaaatc
    35101 tctactcctg tagcttattt gacagttatc aaggaagggt tgaaccgtga aatggtgatt
    35161 aaggatggag atgttctgga gaaatatctg gaagttgata ctttcttgtt tgataagaca
    35221 ggaacaatca caactagtta tcctatagtt gaaaaggtgt taccttttgg agactatagt
    35281 gaggaagata ttctcagaat cagtgcctgt cttgaggaac acatttatca tcctattgct
    35341 aatgccatcg tcaagcaagc tgagatagag ggaattgaac atgaggaaat gcatgggaaa
    35401 ctccaatata tcgcaagcaa ggggatcaaa tctcatatag atggccaacc agttcttatt
    35461 gggaattatg tcttgatgca ggatgagcag attcatatca gttcagaaca aaatgcttta
    35521 attgaagagt acaagagtca ctacaatctc ttattcttgg cttatcaaaa tgaattgatt
    35581 ggaatgttct gcattcatac tcctttgaga aaagaagcaa aaacagcctt ggataaactt
    35641 aaggcacagg ggaaaaaatt gattctggca acaggagaca ccttgattag gacagaggaa
    35701 ttagtcaaag atttgccctt tgatcaggtc tatacagact tgaaacctga tgggaaattt
    35761 gagttagtag agaaactgca gaaagcaggt cacactattt tgatggttgg agatggattg
    35821 aatgactcag cggctttaac tctatcagat atcggtgtgg tgatgaatga gagtgcagat
    35881 atttctaagc agatgagtga tatcttattg ttagataatc gtttggattt cttccaagag
    35941 ttggattcgt tatcatcatc tttgcaaaca ctcatcaaga aaaatattca agataccgtt
    36001 gtcgtaaata gtagtttgat tggctttggc ttatttaatt ggctcagtcc ttcaaatctc
    36061 tctatcttac ataatctaac aaccttgcgc attgtcctgc gtagcctgtc tattaaaaat
    36121 agatagatga gagttattaa cagcaaaaag gagttacctt tctcgaggtt aactcctttg
    36181 tttatagata aatgttgaat ggtttagtaa gtcaatacaa agtatttttt cttcccacga
    36241 cggataacag tcagttcatt ttctaactta tcagcgtcac tcaagacata gtcaaggtct
    36301 tggatgcggt cgccgtttac gtagatggct ccgttttgga cgtcttcacg ggcttggcgt
    36361 tttgagttaa ctataccaga tgagacgagc agttccacga tattgttgtt ttcgtctgcc
    36421 tgtacttggt agtttggcac accacgaagt ccttgtttga gctctttgac agaaaggttt
    36481 ttgatgtttc ctgcaaagag ttgctcagtg atgttaagtg cttctttgta ggcttcttct
    36541 ccgtgaacaa gtgtaacaac ttcacgagcc aagacttttt gagccaagcg ttcgtgtggc
    36601 gctgcttcaa attgtttacg aatatcttca atctcatcaa gtgacaagaa agtaaagatt
    36661 ttcaagaagc gaacagcgtc agcgtccatc acgttcatcc agaattggta catttcgtat
    36721 ggagaagtct tttcgggatt gagccagacg gcatttcctt ctgatttacc aaatttttta
    36781 ccagttgcat ctgtgattag tggaacagtg ataacgtgac cagtcttgtc cgccttacga
    36841 cgaagcaatt cggtaccagc tgtcatattt ccccactggt cagaaccacc gatttgaaga
    36901 gtgacattat ggtcttggtt aaggacgaag aagtcgtacc cttgcatgat ttggtaagcg
    36961 aactcagtgt aagaaattcc tgtttcgatc cgttttttaa cagattcctt actcatcatg
    37021 tagttgaccg tgaagtattt tccaatgtca cggaggaagt caatgaagct gatgctgcca
    37081 aaccagtcgt agttgttgac catgacagcc ttgttttcgc cattttcaaa gtcaagaaaa
    37141 cgagaaagtt gtccttggat agacttgacc cagccatcta ctgtgtcttt tgtttggaga
    37201 ctacgttcag catctttgaa ggacggatct ccgatgagac ctgtagcacc gccaacgagc
    37261 gcataaggtt tgtgacctgc tagttgcaag cgacgacttg tcaagattgc gacaaggtgg
    37321 cctaggtgaa ggctgtcagc agttggatcg tagccagtat aataagaaac ttgaccttct
    37381 tctagggctt tacgcaaagc ttcttcatca gtcgtttgaa atatcaaacc acgctctttt
    37441 agctcatcaa aaatgtgcat gtgtcttttc tcctttttaa aattattgtt tctacctatt
    37501 gtatcacaaa cttgggcaat agcctagcag aatagggaag aaagtggcta ttttattgaa
    37561 aatttccctt ttgtggtata atagatagag tgaggaaatc catgcaaaat caattaaatg
    37621 aattaaaacg aaaaatgctg gaatttttcc agcaaaaaca aaaaaataaa aaatcagcta
    37681 gacctggcaa gaaaggttca agtaccaaaa aatctaaaac cttagataag tcagccattt
    37741 tcccagctat tttactgagt ataaaagcct tatttaactt actctttgta ctcggttttc
    37801 taggaggaat gttgggagtt gggattgctt tggggtacgg agtggcctta tttgacaagg
    37861 ttcgggtgcc tcagacagaa gaattggtga atcaggtcaa ggacatctct tctatttcag
    37921 agattaccta ttcggacggg acggtgattg cttccataga gagtgatttg ttgcgcactt
    37981 ctatctcatc tgagcaaatt tcggaaaatc tgaagaaggc tatcattgcg acagaagatg
    38041 aacactttaa agaacataag ggtgtagtac ccaaggcggt gattcgtgcg accttgggga
    38101 aatttgtagg tttgggttcc tctagtgggg gttcaacctt gacccagcaa ctaattaaac
    38161 agcaggttgt tggggatgcg ccgaccttgg ctcgtaaggc ggcagagatt gtggatgctc
    38221 ttgccttgga acgcgccatg aataaagatg agattttaac gacctatctc aatgtggctc
    38281 cctttggccg aaataataag ggacagaata ttgcaggggc tcggcaagca gctgagggaa
    38341 ttttcggtgt agatgccagt cagttgactg ttcctcaagc agcattttta gcaggactcc
    38401 cacagagtcc cattacttac tctccttatg aaaatactgg ggagttgaag agtgatgaag
    38461 acctagaaat tggcttaaga cgggctaagg cagttcttta cagtatgtat cgtacaggtg
    38521 cattaagcaa agacgagtat tctcagtaca aggattatga ccttaaacag gactttttac
    38581 catcgggcac ggttacagga atttcacgag actatttata ctttacaact ttggcagaag
    38641 ctcaagaacg tatgtatgac tatctagctc agagagacaa tgtctccgct aaggagttga
    38701 aaaatgaggc aactcagaag ttttatcgag atttggcagc caaggaaatt gaaaatggtg
    38761 gttataagat tactactacc atagatcaga aaattcattc tgccatgcaa agtgcggttg
    38821 ctgattatgg ctatctttta gacgatggaa caggtcgtgt agaagtaggg aatgtcttga
    38881 tggacaacca aacaggtgct attctaggct ttgtaggtgg tcgtaattat caagaaaatc
    38941 aaaataatca tgcctttgat accaaacgtt cgccagcttc tactaccaag cccttgctgg
    39001 cctacggtat tgctattgac cagggcttga tgggaagtga aacgattcta tctaactatc
    39061 caacaaactt tgctaatggc aatccgatta tgtatgctaa tagcaaggga acaggaatga
    39121 tgaccttggg agaagctctg aactattcat ggaatatccc tgcttactgg acctatcgta
    39181 tgctccgtga aaagggtgtt gatgtcaagg gttatatgga aaagatgggt tacgagattc
    39241 ctgagtacgg tattgagagc ttgccaatgg gtggtggtat tgaagtcaca gttgcccagc
    39301 ataccaatgg ctatcagacc ttagctaata atggagttta tcatcagaag catgtgattt
    39361 caaagattga agcagcagat ggtagagtgg tgtatgagta tcaggataaa ccggttcaag
    39421 tctattcaaa agctactgcg acgattatgc agggattgct acgagaagtt ctatcctctc
    39481 gtgtgacaac aaccttcaag tctaacctga cttctttaaa tcctactctg gctaatgcag
    39541 attggattgg gaagactggt acaaccaacc aagacgaaaa tatgtggctc atgctttcga
    39601 cacctagatt aaccctaggt ggctggattg ggcatgatga taatcattca ttgtcacgta
    39661 gagcaggtta ttctaataac tctaattaca tggctcatct ggtaaatgcg attcagcaag
    39721 cttccccaag catttggggg aacgagcgct ttgctttaga tcctagtgta gtgaaatcgg
    39781 aagtcttgaa atcaacaggt caaaaaccag ggaaggtttc tgttgaagga aaagaagtag
    39841 aggtcacagg ttcgactgtt accagctatt gggctaataa gtcaggagcg ccagcgacaa
    39901 gttatcgctt tgctattggc ggaagtgatg cggattatca gaatgcttgg tctagtattg
    39961 tggggagtct accaactcca tccagctcca gcagttcaag tagtagttct agcgatagca
    40021 gtaactcaag tactacacga ccttcttctt caagggcgag acgataattc tctaaatgaa
    40081 gtggccaatc aatggattgc cacttttttc ctttcgtgtt acaataaggg tatgaatcag
    40141 tatcagaaaa agattgttaa cggaaaaatt tattcgctcc tatccggctt aatatgggga
    40201 atctgtggaa ttttaggaga gtacttcttt actcattatc aggtgtcttc gggctggatt
    40261 acctctatgc gtttgacact ggcagggagt cttgtactca tttggtctgc aatacaatta
    40321 aaatcgcaag tgctagatat ttggcgagac aagaaaaatt acctgccctt tttagcctat
    40381 gctattttgg ggattttttc agttcagtat tttttctatc tctgtgtaga atactcaaat
    40441 gctacgacag caactatttt acagtttatt agccctgttt ttatcctctt ttacaatcgc
    40501 ttggtttatc aaaaacgagc gtcaaaaagc gctgttttct atgttttggt tgccatgctg
    40561 ggtgtttgct tgatggcgac aaagggagat ctctctcagt tatccatgac gccgctagct
    40621 cttataacag gtttgctgag tgccatgggt gttatgttta atgttatctt gccccaacct
    40681 tttgctaagc gttatggttt tgttcctacg gttgggtggg ggatgatttt ggcaggtttg
    40741 tttagcaatg tcctctcgcc ggtttatcag ctttccttta ctcttgatat ttggagtatc
    40801 ttgatttgcc tcattatcgc tttctttgga acggcttttg cttttttcat ttccatgaag
    40861 gctgtgtcct tggtttctcc tttggtggtt gccgttatca gtgccagtga acctctctct
    40921 tctgctctct tgagtgtttt gttcttagga ttagtagtgg attggtccct ccttctagct
    40981 atagccttga ttattttacc catgatattt ttgtctatag aagaagcgaa agaaagtaga
    41041 taaaaagtct tttcttaatt ctaaaagtgt gctatactag aatagtcaat aaaacacggg
    41101 gagatagttg tgaagagtgt ttttaggttg tatcggtagg ggcttgcttt taccgacagc
    41161 acgttttatc tcacatccct aaaaaatcat acctaaaaac agacaaaaag gtttcaaatt
    41221 tgaggccttt tttggtagaa taggtatcat tataacgaac caggaggcac ctatgactgc
    41281 tacaaaaatg aacgctcaag aaattatcca atttatcgcc aatgctgaaa agaaaaccag
    41341 tgtcaaagta acctttgagg ggcaactcgc aactgctgtg cctagctctg ttgtcaaact
    41401 aggaaatgtc ctattcggag actggaagga cgtggctccg cttcttgaag gtttggtaga
    41461 aaatcaagat tatgttgtcg agcaagatgc tcgtaattct gcagttcctt tgctagataa
    41521 gcgtgctatc aacgctcgta tcgagccagg tgctattatc cgtgaccagg tggaaattgg
    41581 tgacaatgct gttatcatga tgggagctgt tatcaatatc ggtgctgaaa tcggtgctgg
    41641 aaccatgatt gacatgggtg ccatccttgg tggccgtgct atcgttggaa aaaatagcca
    41701 cgttggtgca ggtgcagttt tggcaggtgt gattgagcca gctagtgctg aaccagtccg
    41761 tgtcggagac aatgttctta tcggtgctaa tgcagtggtt atcgaaggag tccaaatcgg
    41821 tagtggttca gttgtcgctg caggagctat cgttacccaa gatgtcccag aaaacgtggt
    41881 agtagcaggt gttccagctc gtatcatcaa agaaattgat gcccaaaccc aacaaaaaac
    41941 agcgctagag gatgcgcttc gtaccttgta attgtaaaag taaaaaagag gcggaaccct
    42001 ttttccagcc tctttctgct ataacggagg acagatagat gttagatttg attcagacta
    42061 gacgagcttt acaccagatt ccagagattg gcttggagga gttcaagact caggcttatt
    42121 tgctggatgt gattgagaaa ttgactacgg gcaaggattt tgttcaaatt cgtacttggc
    42181 ggacagggat tttggtctac ctgcagggaa gtcagccgga gcgaaccatt ggttggcgga
    42241 cagatattga tggtctgcct atcgtcgaac aaacaggtct accttttgct tctcaacacc
    42301 aaggtcgcat gcatgcctgt ggccatgatt ttcatatgac cattgcctta ggctgtcttg
    42361 agcgcgccct tgaggagcaa cccaagaata atctgctctt tctatttcag cctgctgaag
    42421 aaaatgaagc tggtggcatg ctcatgtatg aggacggtgc ttttggagat tggttgccaa
    42481 accaattcta tggtctccat gttcgtccag atttgaaagt cggccagatt gcgactaata
    42541 ctcatacact ctttgcagga acttgcgagg tgaagatccg tttcaaaggc aaaggtggcc
    42601 acgcagcttt tcctcatgaa gccaatgacg ccttggtggc ggctagttac tttgtgaccc
    42661 aagtgcagtc agttgttagc cgcaatgtca acccaatcga gggagcagtg gtgaccttcg
    42721 gcgtttttca agctggaacc accaacaatg tcatcacaga cacagctttt ttacatggaa
    42781 ctattcgtgc cttgacacag gacatgagtc tcttggtgca aaagagggta aaaacagttg
    42841 cagaaggggt tgcagctgcc tttgatatgg aagtcgaagt ggaactcaaa caaggtggat
    42901 acctacctgt tgagaacaat ccagccttgg cgcgtgaact gatggacttc tttgacgaaa
    42961 aagacggaat cgagttgatt gatatcgaac ctgctatgac aggcgaggac tttggttatc
    43021 tcctttcaaa ggttgatggc gtgatgtttt ggctaggtat cgatagtccc tacgctcttc
    43081 atcaccctca aatgagtcct aaggaagaag tcttagctat tggggtggct gcggtgtcta
    43141 gtttcctgaa aaagaaggca gcagaataga gggcttgtct atgaaatcgg aattacgcaa
    43201 gcaagtcttg catgaaatga aggctatatc tcaagagcaa aaacaggcta tagaccaagc
    43261 tttaaccgag cggcttttac accatccctt ctaccaagaa gccaaggtca tcgcaaccta
    43321 cctctctttt tctcatgagt ttcaaacgcg ggaactgatt gagcaggcgc tgaaggacgg
    43381 caagaaggtt ttaataccca aaacttatcc caaggggcgc atggagtttg tggtctatga
    43441 tccgcaacag ttggtaaaaa cttcctttgc cttactggag ccacagggag atgtggaagt
    43501 ggtggatgca tctcagattg atttgattca tgttcctggt ctggctttta cgacgaaagg
    43561 atatcggata ggatacggtg gaggctatta tgaccgctat ctggaacatt tttctggtcg
    43621 tactttgagt acgatatatc cttgtcaaat tcaggacttt atacctgaaa accatgatat
    43681 tcctgttcag gaggtattaa ttgatgaagg aaatctttga tagacgttac cctgtgacga
    43741 gtttcttcct cttagtgacg gccttggtat ttttactaat gttggtcact gcaggcggaa
    43801 actttgacag ggcagataca ttatttcgat ttggagccat gtatgggcca gctattcgcc
    43861 tctttcccga gcaggtttgg cgtctcttgt ctgccatttt tgttcatatt gggtgggaac
    43921 atttcattgt taatatgctt tcactttatt atcttggaag gcaggtagag gagattttcg
    43981 gttctaagca gtttttcttt ctctatcttt tatcaggaat gatgggcaat ctctttgttt
    44041 ttgtatttag tcctaaatcc ttagcagcag gcgcttctac ctctctttat gggctatttg
    44101 ccgcgattat tgttcttcgc tatgcaactc gcaatcctta tattcaacag ctagggcaat
    44161 cctatctgac actttttgtg gttaacatta ttggaagtgt tctgattcca ggaatcagcc
    44221 tagcaggcca tatcggtggt gcagttggtg gcgcatttct agcagttatc tttccagtta
    44281 gaggagaaaa acggatgtat aataccagcc agagattagg agcggtagtc ttgttcgtag
    44341 gactcgccat tttgcttttc tacaagggaa tgggaatgtg acgaagttga aagaaaattt
    44401 gtgtaatgaa aaaagataag gcattttttg aaccttatct ttttatttta ttttattcct
    44461 tctcagtcaa ttcttttcca agttggatga ggtaattctt caaatcatct ttgacttgtg
    44521 ggtgtttgag ggcgtagtcg atggatgttt tcatgaagcc aaacttgtct ccgacatcgt
    44581 aacgagcccc attgaactca cgagcaaata cacgttgtgt tttattgagg gtgtcgattg
    44641 catctgtcag ctgaatttca tttcctgcac ctggagcttg gttttcgagg atttggaaaa
    44701 tttcaggcgt gaggaggtag cgtccgataa tagcaaggtc gctaggagcg tcctctggag
    44761 ctggtttttc aacaaaggtt tcaacactgt aaagaccatc ttttccttcg ccttgcggag
    44821 caataacccc gtaagcagat acttcgtcat gagggactgg catgacagcg atagtagacg
    44881 cgtgggtacg ctcgtagtca tccatgagtt gtttggtaag tggaacagcc ttttcgtctg
    44941 tgatatccat caagtcatca ccaagcataa cgacaaaagg ttcatttccg acgaaagcct
    45001 tagcttgcaa aacagcatct ccgagaccgc gtggatgagt ttggcggata aaatgcagac
    45061 gcatgtcagt tgttttatca actagcttca aaagatctgt tttccctttt tctttgaggt
    45121 tatattccaa ttcgaagttt gaatcaaagt ggtcctcaat agaacgtttt gatttaccag
    45181 tgacaactag aatatcttca atacctgatt tgagagcttc ttccacgata aactggatag
    45241 ttggtttgtc tacgattggc aacatttctt tggcaagggc cttggttgct ggtaaaaatc
    45301 gagttcctag tccagcagca gggatgactg cctttctaac ttttgatgtc ataaaaatcc
    45361 tttctataga gggttaagac cactcatttt ctgctttaaa ttcattgttc atgatgtcat
    45421 aaatggcatc tttgatattg gttccgtgat aaataacttg gtaaatagcc tgtgtaatgg
    45481 gcatatagac tccaagttct tgggctagtt cataggctgc tcgagtcgtt gaaattcctt
    45541 cgattaccat gcccatatta gcttctatat cagctaggga ttctcctcgt ccgagagcat
    45601 ctccagctct ccagttacga gagtggatgg aagttcccgt tacgatcaaa tctcccacac
    45661 cagataagcc gctataggtc aatggactgg ccccgagtgc tacccctagg cgggtgattt
    45721 ctgctaaacc tcgagcgatg atggctgcct tagcattatc accaaatcca agaccatgta
    45781 aagctccagc accgacagca ataatatttt taagagcacc agcagtttca accccgataa
    45841 catccgtatt ggtataaagt cggaagtagt gattactaaa tagcttctga acgtattgag
    45901 ctgtttgtaa atctttagaa gcagcagtta ttaaagttag gtcacgcaca atggtctctt
    45961 ctgcatgact aggccctgaa acaacgacga tatcactacg gagatgttca ggaatttctt
    46021 cttcaagaat ggttgataat cgtttatggc tatcaggttc taatcccttt gatgcgtgca
    46081 tgatgataac cttatggtcc aaggtttgtg caacttgctg ggcaacaagt cgtgtcactt
    46141 ttgttgggac aacaaacaaa atcgcatcca catctttcaa tgtttctgct aagtcggtgt
    46201 aggcaatgat attttcgtct agaacgacat ctttaaagta gtgcttatta gtatggtgtg
    46261 tattaatttc attgatttgc tcgggaagat ttccccaaat acgtacctcg tgtccattgt
    46321 catttaagac ttgtgaaagg gcggttcccc aagaaccagg ccccaagacg gcgacggttt
    46381 gtttttccat gatttcctcc ttgatagagt tctttctctt attctatcat attctagggg
    46441 attttgcact tgcattactg gggtatggtg gaaaaataca caaaaaccga gacgaaggga
    46501 taaactctta gtctttagtt aaagtgagaa gaaatctgat agatagtttg attcagtata
    46561 tgcttataat agaggtaaac aactcaggaa gttcttgtag gttgcgagag tttggctaaa
    46621 aaaatcagtc gttcagaatt ttgtggtgat gagtctgaat taagtatccg tcaattaatt
    46681 agaattgaaa atggagaatc cagaccaaca ctaacaaagt taaaatatat cgctgaacgt
    46741 ttgggggttg aagattacaa gttgatgcca agttatatag agttggataa ggaataccta
    46801 gaattgaagt atttcttgat gaggactcct acatacgaag atgaaactat cgcccaaaag
    46861 aaagagagtg ttttgataag atttttgaag agtattatga taggctacct gaggaagaaa
    46921 gatttatcat cccaaattat tcatatctgg cactaacgaa ctacacagtt caaaaattac
    46981 cagaaaagct agttgaaata ctgtccttct ggtgattttg cttaatttct gatgttcgag
    47041 acataattga ggagatttgt cttgattttt tcctaaaaaa gggcagactc ctcccttggt
    47101 ttgatgctcg atttttaaaa taaggtgact tattctgtta ggcatgtttt cccctgccct
    47161 ctagctctat atggaacagg gagtatcaga tttatctggc agattgtatc aaataaggct
    47221 tgaaactgtt ttgaataggc atatagactt gagaatttga gaatatgtcg tctagcagta
    47281 gagacatatt ttccggcaat atgaaggaag agacgtcgaa aacgcttgat agtcaaggcc
    47341 tttacttcat caccagctag ctgctttaaa aagaggtaga gattataagc cagacagccc
    47401 atcatcatac gtacttcatt cttaatcatg gtcgaactat ctgtcttatc cccaaagaat
    47461 cctgctttcc tttctttgat aaagttttca gcattccctc gttcacgata gaattgaaac
    47521 agctcctcac tatctacact gcttaaattg ttcaaatgaa cttttttaca accataaaaa
    47581 agagcactta atcgtactct tctcttttaa ggattagttg aattaattca aatccaccag
    47641 ttctcctgtt tctaggtaga ctaggcgttc acaaatgtta gcaatgtaat cagcgaagcg
    47701 ttccagatgc cctatgatat aaaggtattg agttccattg ggaattgagg tttcttggtc
    47761 tttcataagt ccaatgattt cctttgataa ggcataataa tattggtcaa tctgttcatc
    47821 tttttgagca atactaatag cttttgaggc ttggtgcaaa ggaaaggcaa ccaataaatc
    47881 ggctagcatg ctgagggata atttacccat ttggtgtaac tgttcttcgt caggggctag
    47941 ttgattttct tttagttgca aaacagcttt agcaatgcct gccatatggt ctcccatacg
    48001 ttcaaggtct gaacaagaag acatgatgct aatcacaaat cgaaggtcag acacttgtgg
    48061 ctgctgcaag gccaacaaac gggcacaggt caattcgata gcgctttgac cttggttgat
    48121 agcatgatcc ttattgataa ttagctctgc catctccttg tctttggagg ctaaggccag
    48181 taaggctttt gaagctgttt caaggacaag ttgccctagt cctaaaaagg attgttctaa
    48241 ttcatgcaat tctaagtcaa attgatttct cataggtttt tccttcctta tccgaaccgt
    48301 cctgaaatat agtcttctgt gcgctgatct tttggattgg taaacacgtc aacggtatct
    48361 ccaaattcgc aaatttctcc tgttaagaaa aaagcagttt tatctgaaat acgtgaagct
    48421 tgttgcatgt tatgggtaac aatgataatc gtataatcct tttttagttg ctgaatgagg
    48481 tcttcaattt ttaaagtgga gatagggtct aaggctgaag tcggctcatc cattaacaga
    48541 atatcaggtt ctactgctaa agctcgcgca atgcaaaggc gttgctgctg accgccagat
    48601 aaggacatgg cactcttttt aagatcatct ttgacttctt cccaaatggc tgcccctttt
    48661 aaagatttct ccactaaggc atctaattgt tttttgtctc gaataccatg tgtccttggg
    48721 ccataagcca cgttatcata gatagacatg gcaaagggat taggctgttg aaaaaccatc
    48781 cctacacgct tacgtagctg attaaggttg aatttgctac tataaatatc ttgctcatct
    48841 aagaggactt ggccttcaat atggcaagaa ggaaccaaat cgttcatccg gttaagggtt
    48901 tttagaaaag ttgatttgcc acaaccagat gggcctatca aggcagtaat ctgtctttct
    48961 ggtaattgaa tcgaaatatt ttttaaggct tgaaaatccc cgtaaaataa gtctaggtgt
    49021 ctgactgaaa atgttcccat actaggaagc tcctttcaca agttttcgag ataataagct
    49081 tgatagagta tttatcatta aaacagtaat aatcaaaatc acaccggtag cataggcttc
    49141 attgacatgt agcccctcac ttgacagcat atacatatgc agggctagag aacgacctga
    49201 agacatgaga ctacttggcg tattggtaga ggtacctaat gtatacatga gggcagctgt
    49261 ttcaccaacg atacggccaa tagctagtat cactccagct aaaatacctg gcatggcaac
    49321 tggtagaaca attctaaaaa cagtccgtaa cttacctgcc ccaagtccat aacttgcttg
    49381 acgcatgcta tcactaacag ataaaagggc ttcttctgtt gagcgaataa tgactggcaa
    49441 caccatgata actgaggtta agattcctga taacagagag tattgaaaac ctaagaagac
    49501 tacaaagaag agcatgccaa acagaccaaa aacaatggaa ggaatcccag acaaggtatc
    49561 tgaggccaat cgcatgattt taacacaaag ggaatctttt tttgtatatt ccacaagata
    49621 aaaaccagca aaaatcccta tgggcaaggc taaaagaaga gcaccaaaga ccagaataac
    49681 ggtggaaata atcgctggca taagggaaat gttctcagaa gtataagtcc aagaaaagag
    49741 ggatagactt agatgaggta agcctttgat gaggataaaa ccaatgatta aaaagagaga
    49801 gccaaaggtt aaagctgaaa aacaataaac gagaagtttt agcaggtatt tactcataag
    49861 atgattttcc tttcaagtag gcaaagtagg cattaatcaa gagaataagg aaaaagagaa
    49921 ctgctgaggt tgcaataagg gcttccctat gctgacctga tgcgtaagcc atctccagaa
    49981 caatattggt tgttaaggtt ctggttcctg aaaagagtcc acttggaata attggctggt
    50041 tgcctgccac caaaataact gccatggttt cacctactgc gcgaccgatt cctaaaataa
    50101 ctgctgataa aataccagat ctcgcagctg gcaagatgac actaaaaata ctccgttcat
    50161 gactagctcc tagagccaag ctaccagaat aatacgtttt gggaactgtt cggatagcag
    50221 attctgacaa actgataatg gttggcaaaa tcattattcc taatagtaac gaagcggtta
    50281 ggacactcat gccatttcct aaaaagcttc taatccaagg caccaataat tgtaggccga
    50341 aaaaaccata aacaatagat ggaatggctg ccatcaagtt gatagctgat tttaagaagc
    50401 catagacggg ctttggacaa taataaacca taaacaccga tgtcaagatg cctgttggca
    50461 ccccaatcac aatcgctcct aaggtaatta ataaggaacc aacgatcatt ggtaaaatac
    50521 catagcttgc cggaatgttc gttggcgacc aatcactgcc taataaaaaa cgggcaaagc
    50581 cgtagttagc tatgaaaggt aagccattac taaaaataaa gaaacagatt agcaaaatag
    50641 ctacaacagc tactgttgca ctcatgaaaa aaattgccct aaaaactgct tctttgaagg
    50701 cttgttttgt cacatcttgt cctttctagt gaagaaagta agggagatac gacacctccc
    50761 tacttgcctt ctttatctta ttgtacgatg aaacgtctgc atctctttag agatttatgg
    50821 agcaaacatt ttatttaatc ttgtcccagg tggttaattt gccactaaaa acgtctgcaa
    50881 gttcagccat actgacttgg cttgccttat tgtcattatt gaccacaaca gcaataccgt
    50941 ctaaagcaat agcatcatgg gtgagactct taccttcttc aggagttaat tccctagaaa
    51001 ccataccaat atcagcggtt ttctccttaa cagcggtaat acctgctgaa gacccattag
    51061 aggtaatatc aatcgtaact tctggatttt cttttttata agcttctgct aatttttcca
    51121 ttaaagaaga tactgaagtg gaacctacaa cagacaactt gcctgataag tgttggcttg
    51181 tatattctgt ggtttcggtt ttagcttcaa taaatttatt atctgtgacc acttgttgac
    51241 cttgtttgga gtggataaag ctgataaaat cttgacctag cttggaaaga ttagaagacc
    51301 aaacaatgtt gaagggacgt tgaagagggt atttaccatc taaaactgtg tctcgactag
    51361 ccttgacacc atcaatctct aaagctttga cagatttcgt taaagatccc aaggagatgt
    51421 agccgatagc attagcattc ccttgaactg ctgagagaac accttctgta ctattttgaa
    51481 tcacagctgt tttggcagtg ttgtcaattt ttttatcacc gtcttttttg agaatccctg
    51541 tgatttctgt gaaggcaccc cgtgttccag agccattttc tcgtgaaatc acctcaatcg
    51601 ttcctgaagc tgactgtttg gaagcagctg actgattgcc acaggcaaca agcccaaatc
    51661 ctgataagcc aatggctgca agagtaagca tttttttgaa tttcataata atcaccttta
    51721 tctctatgta tttttcttgt gtaggcttac tacatttata gtctaacaag tctttgtaaa
    51781 ggtttatccc tgattcatgt aaagattgtg taaagaatca aaaaaagcca cttttgaaaa
    51841 atggctgctc ctaaaaatag ctttaaaaat tattagtcct gtgcgaaaga ttggttagga
    51901 agaaaaatcg tgaagcaact gcctctgcca agctgactcg tcaccgtgac ttggccacct
    51961 aataattgac tgagttcttt gacaatggca aggccaagac cagtgccacc agtttgtctg
    52021 cttcgacctt tattaactcg gtaaaaacgt tcaaaaatac gatcctgctc taattgacta
    52081 ataccaatcc ctgtatctga tacagaaatc ttaatgcctt cgttcacctt ttgggtcttg
    52141 acctcaattt ttcccccttg ttcagtgtaa cggatggcat tggataaaag attgagtaag
    52201 atttgggaaa gtaattgact atctgatacg agggtgacat catctggcac ctgcaccttt
    52261 agctgtaaat ccttcttctt gagctgaggt tgcaagcttt gagtcaaatc ctgtacaaat
    52321 tctgccaaag aaagggtcgt ccattgtata ggcatttgtt gagccttaga taaggtaaga
    52381 agatgctcaa caatatgctc aagacgcaag ctttctttgt aaataatgtc tagaaagtca
    52441 tccttgagcg cttcttcttc agctgacatc cccttaatgg tttcagcaaa gcccttaatc
    52501 gaagtaactg gtgtcctcaa ttcatgggag gcatttgaga caaaggctaa atttaacttt
    52561 tcataagttc taatcgttgt taaatcatat agcaagacga gcacagcttc cacagattgg
    52621 gtggggctaa aaacgggaac tgctgtcact tctaaaatca agtcaccctc atgaaaccca
    52681 cttacttctt gttttaacct tgttttttga tcaaaggctt ggtgaactaa atttcgaata
    52741 tccatccgtt tgaggtcatc aagtgaactt atgtcgccgt ccacatcggg aaaataatga
    52801 ggcagagagc gactggataa taacatctga ccttgagcgg aaactaaaaa cgtccccatg
    52861 gttaggtgcg acagaagaac ctccattgtt tcggctaggt ccttgtattg ctgatcctgt
    52921 tgggagactt tggtttttag ggcagacaca tactgagcca aagactttaa gtcttcttgc
    52981 cctttttcta aaaagtattc actactggtc aagagaggtt ggtgcaaggt ctcaaaagca
    53041 acttcccatt tccaaaggca aaagagccag tagccacctt gtcccaaaga aagggctaga
    53101 agaaagagac cgatgccttt actgatccaa gttaatacca tccctgcaat cagaatgagg
    53161 ctaacactta gattgactag ccaaaattga aggtagcgtt tcatctataa ctccttgaac
    53221 ttataaccat aaccccgaat ggttcgaata aattgagggg ctttaggatt gtcttcaatt
    53281 ttttccctca acttaccaat atgaacgtcc accaaacgtg tttcctgccc aaagtcatac
    53341 ccccagatac gttccaaaag acgctctcta gtcagtgtca tgttgggatg tttcataaga
    53401 tagagcaaga gttcaaattc ttttggggtc aaactcagta acttattcgc cttgtagact
    53461 tcatgacgct cagggtatac tttcaaggtc ccaaatagcc aagaatcgtc agcgatatta
    53521 tctgaatcat ctccttcttg ttctccttta gttcgcctga ggacagcctt gacacgcgcc
    53581 agcaattctc tagggctaaa aggtttggtc aggtagtcat cagcccctaa ttccaaggcc
    53641 aaaaccttat caaattcatc acttttcgca gaaaccatca taattggagt tttgacgcct
    53701 ttggctctca gccgcttaca aacttccatg ccatctaatt gtggtaacat gatatcaagc
    53761 aagataaaat caaagggttc tgtttctgcc aaagctaagg ccttccgtcc atttgttacc
    53821 aattgagtag aaaagccttc cttacttaaa tggtagtcaa gcaatttcag aatgtgttct
    53881 tcatcatcca ctaataagac ttgttttgtc atctattatc tcctattggt aacattataa
    53941 cacaattatc agaaatccta acattgctaa atcagattaa atttgcttat caagactagt
    54001 atctggtcaa acgctcaatc atctccttgt gctctggata ggtcgccagt agatctaccc
    54061 tttcaaataa ttcaaaatcc tcaaattcaa aaccaggagc aacaagacaa gaaaccagag
    54121 catcatcctt atcaactgtt gatccccaaa tagtgccctt aggaacacag tagtgaagtt
    54181 gttgcccttt ggatatgtcc aggcctaaag tgactgcttc gtagtgacca tctgctgtaa
    54241 tcatgtgaac agtaagtggg gattctgcat gaaaatacca gatttcatct gctgtcaatc
    54301 ggtgaaaatg tgaaggattc gtttcttctt aataagaaat aaatactggt ataaagcgcc
    54361 cttcccttac cagcaaggtt tatagtgtct gaagcttttt ttgtttgtct aaaatagcca
    54421 ccttcaacat ggggagctaa ctctagagtt cttatcaagt cttctttatc cgtcggagcc
    54481 aatgggttga agtaactctt gttcaaagtg gtgttacgat ttcaagaact cctctcagtt
    54541 ctgaggacac ggtaatgatt gatgcgacgg aagtacaaat caatcgccct aaaaaaagaa
    54601 ttagcgaatc attctggtaa aaagaaattt cacgctatga aggctcaagc gattgtcaca
    54661 agtcaaggga gaattgtttc tttggatatc gctgtgaact atagtcatga tatgaagttg
    54721 ttcaaaatga gtcgcagaaa tatcggacaa gctggtaaaa tcttggctga cagtggttat
    54781 caagggctca tgaagatata tcctcaagca caaattccac gtaaatccag caaactcaag
    54841 ccgctaacag ttgaagataa agcctgtaac catgcgctat ctaaggagag aagcaaggtt
    54901 gagaacatct ttgccaaagt aaaaacgttt aaaatgtttt caacaaccta tcgaaatcat
    54961 cgtaaacgct tcggattacg aatgaatttg attgctggta ttatcaatca tgaactagga
    55021 ttctagtttt gcaggaagtc tattatttgg ttaggtgaat tagtgaagcg tttaggcaag
    55081 tgtctctggt tacgacgtca tggcctctaa atcgattata tttaggggtc atgactagtg
    55141 aagcagttag ctagttcgca tataagcggc tagcgtctaa caattaggaa ctttagttcc
    55201 aataacttta agattacgac gttttaggac ataaatcgat catatttatg tcctaaaact
    55261 agtgaagcgc ctagccaaag tccgaatagg atttggcgtt agttacttag attgctttgc
    55321 aatcaagtaa ctttggcgat ttacatcttc tctggtgctt ctactccaag caagcgaagg
    55381 gcttctttga gaacgactgc ggttgcgtag ctgagggcta gacggctgtc gcgttctggg
    55441 ctttcatcca agatacgtgt atgtgcatag tatttgttaa aggattgagc caggctaatt
    55501 gcaaatttag caatgataga aggttcaaag ttatctgccg cacggttgat aatacgtggg
    55561 aagtcttgaa tgagtttaat gatttcccag ctttcagtat cattcaagct atagttgcca
    55621 gctgtttctg gtttgaaatc ggctttgcgt aagatagatt ggatacgagc gtaggcatat
    55681 tgaacgtaag gtccagtttc accctcgaag gataccatag cttctaggtc gaagtcgtat
    55741 ccatttgtac ggtcagtttt gaggtcatag aatttaatgg ctccaacccc aacagcatgt
    55801 gctacttggt ctttgttttc tagttcagga tttttagcct cgatttggac cttggcacgg
    55861 ctaacagcct ctgcaacagt aggctctagc aagatgacat tccctttacg agtagagagc
    55921 ttcttccctt cttttgtaac cagaccaaaa ggaacgtggg taatgtcgtc actccaatcg
    55981 tagcccatct cttgcaagac agctttgagt tgtttaaagt gggcagattg ctcttgacca
    56041 acgacgtaga tagatttagc aaattggtat tcatttttac ggtagagggc tgcagccaag
    56101 tcacgtgtga tatagagagt tgcaccatca gacttcttga tgagggctgg atgttcaatt
    56161 ccatatttct caagattcac aacttgggca ccttctgatt caagaagtag tcctttttca
    56221 gaaagaatgt ctacaactgc atccatctta tcattgtaga aggcttctcc gttatagctg
    56281 tcaaattcaa ccttcaattc attgtaaagg cggttaaatt ccactaaact ttcatcgcgg
    56341 aaccattgcc aaagagcgag agcttcctca tctccatttt caagtttacg gaaccattcg
    56401 cgcgcttctt catccaagct agggtcattt tcagcttcag cgttgatgcg gacatagagt
    56461 ttaaggagtt catcgattgg atgagctttt acagcttctt cgtcgcccca ttttttatag
    56521 gcaacaatca acatcccaaa ttgtttaccc cagtctccca aatggttgac cttgaccgtt
    56581 tgataaccga ttttttggaa aatatgtgac aagctatctc cgataacagt tgaacgcagg
    56641 tggccaatag aaaatggttt agcgatattc ggactagaca tgtcgataac aacattttct
    56701 tgtttaccaa tattttggtc agcatagtgt tctttttcag tggtaacagc ttgcaatact
    56761 tgagcagaaa tggcagattt atcaaggaaa aagttaacgt aaggtcctgt tgcgacaact
    56821 ttttcaaagg cttggctgtt cattttttca gccagttcag ccgcaatcat ttgtggtgct
    56881 ttacgttcga cttttgcaag agaaaaagca gggaaagcaa tgtctcccat ttctgagttt
    56941 ttaggggttt ccagtaactt taaaacagcc tcttggtcca ggctatcaat gatgctagat
    57001 aattcgctag caatcaattc ttttgtattc attaagagct cctttttgga cttttctact
    57061 attttatcac aattttaaag aaagaagaaa aaatttttga aatctcctgt ttttttggta
    57121 taatatggtt ataaatatag ttataaatat gcacgcaaga ggattttatg agaaaaagag
    57181 atcgtcatca gttaataaaa aaaatgatta ctgaggagaa attaagtaca caaaaagaaa
    57241 ttcaagatcg gttggaggcg cacaatgttt gtgtgacgca gacaaccttg tctcgtgatt
    57301 tgcgcgaaat cggcttgacc aaggtcaaga aaaatgatat ggtgtattat gtactagtaa
    57361 atgagacaga aaagattgat ttggtggaat ttttgtctca tcatttagaa ggtgttgcaa
    57421 gagcagagtt taccttggtg cttcatacca aattgggaga agcctctgtt ttggcaaata
    57481 ttgtagatgt aaacaaggat gaatggattt taggaacagt tgctggtgcc aataccttat
    57541 tggttatttg tcgagatcag cacgttgcca aactcatgga agatcgtttg ctagatttga
    57601 tgaaagataa gtaaggtctt gggagttgct ctcaagactt atttttgaaa aggagagaca
    57661 gaaaatggcg atagaaaagc tatcacccgg catgcaacag tatgtggata ttaaaaagca
    57721 atatccagat gcttttttgc tctttcggat gggtgatttt tatgaattat tttatgagga
    57781 tgcggtcaat gctgcgcaga ttctggaaat ttccttaacg agtcgcaaca agaatgccga
    57841 caatccgatc cctatggcgg gtgttcccta tcattctgcc caacagtata tcgatgtctt
    57901 gattgagcag ggttataagg tggctatcgc agagcagatg gaagatccta aacaagcagt
    57961 tggggttgtt aaacgagagg ttgttcaggt cattacgcca gggacagtgg tcgatagcag
    58021 taagccggac agtcagaata attttttggt ttccatagac tgcgaaggca atcaatttgg
    58081 cctagcttat atggatttgg tgacgggtga cttttatgtg acaggtcttt tggatttcac
    58141 gctggtttgt ggggaaatcc gtaacctcaa ggctcgagaa gtggtgttgg gttatgactt
    58201 gtctgaggaa gaagaacaaa tcctcagccg ccagatgaat ctggtactct cttatgaaaa
    58261 agaaagcttt gaagaccttc atttattgga tttgcgattg gcaacggtgg agcaaacggc
    58321 atctagtaag ctgctccagt atgttcatcg gactcagatg agggaattga accacctcaa
    58381 acctgttatc cgctacgaaa ttaaggattt cttgcagatg gattatgcga ccaaggctag
    58441 tctggatttg gttgagaatg ctcgctcagg taagaaacaa ggcagtcttt tctggctttt
    58501 ggatgaaacc aaaacggcta tggggatgcg tctcttgcgt tcttggattc atcgcccctt
    58561 gattgataag gaacgaatcg tccaacgtca agaagtagtg caggtctttc tcgaccattt
    58621 ctttgagcgt agtgacttga cagacagtct caagggtgtt tatgacattg agcgcttggc
    58681 tagtcgtgtt tcttttggca aaaccaatcc aaaggatctc ttgcagttgg cgactacctt
    58741 gtctagtgtg ccacggattc gtgcgatttt agaagggatg gagcaaccta ctctagccta
    58801 tctcatcgca caactggatg caatccctga gttggagagt ttgattagcg cagcgattgc
    58861 tcctgaagct cctcatgtga ttacagatgg gggaattatc cggactggat ttgatgagac
    58921 tttagacaag tatcgttgcg ttctcagaga agggactagc tggattgctg agattgaggc
    58981 taaggagcga gaaaactctg gtatcagcac gctcaagatt gactacaata aaaaggatgg
    59041 ctactatttt catgtgacca attcgcaact aggaaatgtg ccagcccact ttttccgcaa
    59101 ggcgacgctg aaaaactcag aacgctttgg aaccgaagaa ttagcccgta tcgagggaga
    59161 tatgcttgag gcgcgtgaga agtcagccaa cctcgaatac gaaatattta tgcgcattcg
    59221 tgaagaggtc ggcaagtaca tccagcgttt acaagctcta gcccaaggaa ttgcgacggt
    59281 tgatgtctta cagagtctgg cggttgtggc tgaaacccag catttgattc gacctgagtt
    59341 tggtgacgat tcacaaattg atatccggaa agggcgccat gctgtcgttg aaaaggttat
    59401 gggggctcag acctatattc caaatacgat tcagatggca gaagatacca gtattcaact
    59461 ggttacaggg ccaaacatga gtgggaagtc tacctatatg cgtcagttag ccatgacggc
    59521 ggttatggcc cagctgggtt cctatgttcc tgctgaaagc gcccatttac cgatttttga
    59581 tgcgattttt acccgtatcg gagcagcaga tgacttggtt tcgggtcagt caacctttat
    59641 ggtggagatg atggaggcca ataatgccat ttcgcatgcg accaagaact ctctcattct
    59701 ctttgatgaa ttgggacgtg gaactgcaac ttatgacggg atggctcttg ctcagtccat
    59761 catcgaatat atccatgagc acatcggagc taagaccctc tttgcgaccc actaccatga
    59821 gttgactagt ctggagtcta gtttacaaca cttggtcaat gtccacgtgg caactttgga
    59881 gcaggatggg caggtcacct tccttcacaa gattgaaccg ggaccagctg ataaatccta
    59941 cggtatccat gttgccaaga ttgctggctt gccagcagac cttttagcaa gggcggataa
    60001 gattttgact cagctagaga atcaaggaac agagagtcct cctcccatga gacaaactag
    60061 tgctgtcact gaacagattt cactctttga tagggcagaa gagcatccta tcctagcaga
    60121 attagctaaa ctggatgtgt ataatatgac acctatgcag gttatgaatg tcttagtaga
    60181 gttaaaacag aaactataaa accaagactc actagttaat ctagctgtat caaggagact
    60241 tctttgacaa ttctccactt ttttgctaga ataacatcac acaaacagaa tgaaaaggag
    60301 ctgacgcatt gtcgctccct tttgtctatt ttttaaggag aaagtatgct gattcagaaa
    60361 ataaaaacct acaagtggca ggccttggct tcgctcctga tgacaggctt gatggttgct
    60421 agttcacttc tgcaaccgcg ttatctgcag gaagtattag acgccctcct tactgggaaa
    60481 tatgaagcta tttatagtat cggggcttgg ttgattggtg tggccgtagt cggtctagtt
    60541 gctggtggac tcaatgttgt cctcgcagcc tatattgccc aaggagtttc atccgacctt
    60601 cgggaggatg ccttccgtaa aattcaaacc ttttcttatg ctgatattga acaatttaat
    60661 gcgggaaatc tagtcgttcg aatgacaaat gatatcaacc agattcagaa cgttgtcatg
    60721 atgaccttcc aaattctttt cagacttccc ctcttgttca tcggttcgtt tatcctagcg
    60781 gttcaaacct taccttctct gtggtgggtg attgttctca tggtagtctt gatttttggt
    60841 ttgactgctg tcatgatggg aatgatgggg cctcgttttg ccaagtttca aacccttctt
    60901 gagcgcatca atgccattgc caaggaaaat ttacgtggcg ttcgtgtggt caagtccttt
    60961 gtccaagaaa aagagcaatt tgctaagttt acagaggtct cagacgagct ttttggtcaa
    61021 aacctttaca ttggttatgc cttttcagta gtggaaccct ttatgatgtt ggttggttac
    61081 ggggcggtct tcctctctat ttggctggtc gcgggaatgg ttcagtcgga tccgtctgtt
    61141 gttggttcca tcgcttcttt tgttaattac ctaagccaga ttatctttac cattgttatg
    61201 gttggatttt tgggaaattc tgtcagccgt gccatgattt ccatgcgtcg tattcgagaa
    61261 attcttgacg cagagccagc tatgaccttc aaggatatcc cagatgaaga gttggttgga
    61321 agccttagct ttgaaaatgt gacctttacc tatccaatgg acaaggaacc gatgctgaaa
    61381 gatgtgagct ttactattga acctggtcaa atggttggtg tagttggagc gactggtgca
    61441 ggaaagtcaa ccttggctca attgattcca cgtctctttg atccacagaa cggggccatt
    61501 aaaatcggtg gcaaggatat tcgagaagtg agtgaaggaa ccctgcgtaa aacagtttcc
    61561 atcgttctcc aacgtgccat tctttttagt ggaacgattg cagataactt gagacagggg
    61621 aagggaaatg ctactctatt tgaaatggag cgcgcagcca atattgccca ggctagtgaa
    61681 ttcattcatc gtatggagaa aacctttgaa agtccagttg aagaacgggg aaccaatttc
    61741 tctggtggac aaaaacaaag gatgtcgatt gcgcgtggga ttgtcagcaa tccacgtatt
    61801 ctgatttttg atgattcgac ctcagccttg gatgccaaat cagagcgctt ggtgcaagaa
    61861 gctttgaata aggacttgaa ggggacgaca accattatta ttgctcaaaa aattagctcg
    61921 gttgtccatg cagacaagat cttggttcta aatcaaggac gattgattgg tcaaggtacg
    61981 catgcagact tggttgccaa caatgccgtt taccgtgaaa tctatgaaac acagaaatga
    62041 aagacaaact ataagaaaag tcaatagttt tatctaaact atttcttatt tcaatttgat
    62101 gatttggcga tgattttaga gcacggcaaa aggcccttga aaaagtccat tttttcaaag
    62161 gtaatcctgt gttaatttca gaaattacat cactttttgt tcgtcaaatg gcagctcttt
    62221 ttttaggata taaaacaggg ttcggataag tttttttgca aggtggatga tggctacatt
    62281 gtaatgtttt ccttgttcta atttagtctt aagataggcc ttaaaagcag gcgaaaagcg
    62341 agggcatgct ttggcagctt gtatgagtac ctaccgcaga tgaggggaac tccgtttgac
    62401 cattcttcct gctaaatcaa tctgatctga ctgataaata gaagaatcca gtccagcgaa
    62461 agcttgtaat tgagcaggat tatcaaaggc atgaatattt cgaatctcag ctaaaatgac
    62521 cgcccctaaa cgatccccaa tcccagtaac cgtcgtgatg accgagttga actcagccat
    62581 caagtcattg acacatgttt ccgccttgtc aatgagcccc ttgtaatgtt tgatgttttc
    62641 attacacgag ataaaacgtc tatgcgttat caaactcatt accaattaaa acaaaaagct
    62701 gtggttagat cctttcggaa attgtcaagc gattggagga aatgaactaa tccacagcgg
    62761 cttattccaa gtataccact tgggctttgg cagtagctaa ctgcgctaaa tataatataa
    62821 ggaggagtaa aatgaagaca gttcaatttt tttggcatta ttttaaggtc tacaagttct
    62881 catttgtagt tgtcatcctg atgattgttc tggcgacttt tgcccaagcc ctctttccag
    62941 tcttttctgg acaagcggtg acgcagctag ccaatttagt tcaagctcat caaaatggca
    63001 atccagaact tgtatggcaa agcctatcag gaatcatggt caatcttggc ctgctggttt
    63061 tggttctatt tatctctagt gtaatataca tgtgtctcat gacgcgcgtg attgcagaat
    63121 cgaccaacga gatgcgcaaa ggtctctttg gtaagcttgc tcagttgacg gtttctttct
    63181 ttgaccgtcg acaagatggc gatatcctgt ctcattttac cagtgatttg gataatatcc
    63241 tccaagcctt taacgaaagc ttgattcagg tcatgagcaa tattgtttta tacattggtc
    63301 tgattcttgt catgttttcg agaaatgtga cgctggctct catcaccatt gccagcaccc
    63361 cattggcttt ccttatgctg attttcatcg tgaaaatggc acgcaaatac accaacctcc
    63421 agcagaaaga ggtagggaag ctcaacgcct atatggatga gagcatttca ggccaaaaag
    63481 ccgtgattgt gcaaggaatt caagaggata tgatggcagg atttcttgaa caaaatgagc
    63541 gcgtgcgcaa ggcaaccttt aaaggaagaa tgttctcagg aattcttttc cctatcatga
    63601 atgggatgag cctgattaat acagccatcg tcatctttgc tggttcggct gtacttttga
    63661 atgataagtc tattgaaaca agtacagccc taggtttgat tgttatgttt gcacaatttt
    63721 cacagcagta ctaccagcct attatccaag ttgcagcgag ttggggaagc cttcagttgg
    63781 cctttactgg agctgaacga attcaggaaa tgtttgatgc agaggaggaa atccgacctg
    63841 aaaaggctcc aaccttcact aagttgcaag aaagtgttga aatcagtcat atcgattttt
    63901 catacttgcc tgataaacct attttgaaag atgtcagcat ttctgcccct aaaggccaga
    63961 tgacagcagt tgttgggccg acaggttcag gaaaaacgac tattatgaac ctcatcaatc
    64021 gcttttatga tgttgatgct ggtggtattt attttgatgg taaagacatt cgtggctatg
    64081 acttagatag tcttagaagc aaggtgggaa ttgtattgca agattcggtc ttgtttagcg
    64141 gaacgattcg agacaatatc cgatttggtg tgccagatgc tagtcaggaa atggttgagg
    64201 tagcagcaaa agcaacccac attcacgact atatcgaaag tttgcctgat aagtacgata
    64261 ctcttattga tgatgaccag agcatctttt caacagggca gaagcaattg atttcaatcg
    64321 ctcgaaccct gatgacagat ccagaagttc tcattctcga tgaagcaact tcaaacgtag
    64381 atacggtgac agaaagcaag attcagcatg ccatggaggt ggttgtagca ggtagaacta
    64441 gtttcgtcat tgcccaccgc ttgaaaacca ttctcaatgc agatcagatt attgtcctta
    64501 aagatggaga agtcattgaa cgtggtaacc accatgaact tttgaagcta ggtggctttt
    64561 attcagaact ctatcacaat caatttgttt tcgaataaga aagaagttgt cctatgtggg
    64621 cagctttttc ttgtccataa aaaatgttta tcacagcctt aaaaaaaaca tattagacga
    64681 aagtcatttt gagtgatatg ataggactat cgttagcatt cgaaaggaga ggcatcatgg
    64741 ctagaacggt tgtaggagtt gctgcaaatc tatgtcccgt agacgcagaa ggcaaaatca
    64801 ttcattcatc tgtatcttgt agattcgcag agatcattcg tcaagtcggt ggtctccctt
    64861 tagtcattcc tgttggtgat gagtcagttg tacgtgatta tgtggaaatg attgacaaac
    64921 tcattttgac aggaggccaa aatgttcatc ctcagtttta tggagagaag aagaccgtcg
    64981 agagcgatga ttacaatctg gtccgtgacg aatttgaatt ggcactcttg aaggaagcgc
    65041 ttcgtcagaa taaaccaatt atggcaatct gtcgcggtgt ccaacttgtc aatgttgcct
    65101 ttggtggaac cctcaatcaa gaaatcgaag gtcactggca aggactacct ttcgggacat
    65161 ctcactctat tgagacagta gaaggaagcg tggtggctaa gctatttggg aaagaaagtc
    65221 aggtcaactc agtccatcgt caaagcatta aagatttggc acctaatttc cgtgtaactg
    65281 ctattgattc aagagaccaa actattgaag cgattgagtc tatcgacgag caccgcatta
    65341 tcggtttaca gtggcatcca gagtttctgg ttaatgaaga agatggcaat ttagaattat
    65401 ttgagtattt attgaatgaa ctgtaacgat tggaacatct ggtcgttctt tcttttattt
    65461 tttcaaaatt tttagaatgt ggcattttac gcaaacgttt gaattctgat aaaaattgga
    65521 gaaattcgac aaaaaaactt gaaaaaaacg aaggtaagcg ttatgataga aaagaagaaa
    65581 tattggagga agaacatgtc acatattaaa tttgattatt caaaagtttt agacaaattt
    65641 gttgcaccac atgaagtgga atacatgcaa tcacaagtaa cagcagcaga tgaattgatc
    65701 cgtaaaggaa ctggtgctgg tagcgacttc ttgggatggt tggaccttcc tgaaaaatac
    65761 gaccgcgaag aatttgaccg catcttgaaa gctgctgagc aaatcaagtc agacagcgat
    65821 gttttggttg tgatcggtat tggtggatct taccttggtg caaaagcagc aatcgacttc
    65881 ttgaatcacc attttgctaa cttgcaaaca aaagaagaac gcaaagctcc acaaatcctt
    65941 tacgcaggaa actcaatctc atctacttac cttgctgact tggtagagta tgttgcagac
    66001 aaagacttct cagtaaacgt aatttctaaa tcaggtacaa caactgaacc agcgattgct
    66061 ttccgtgtct ttaaagaact cttggttaag aaatacggtc aagaagaagc taacaaacgt
    66121 atctatgcaa caactgaccg ccaaaagggt gctgttaagg ttgaagcaga cgctaacggt
    66181 tggggaacat ttgttgttcc agatgatatc ggtggacgct tctcagtatt gacagccgtt
    66241 ggtttgcttc caatcgcagc atcaggagct gacataaaag ctcttatgga aggtgcgaat
    66301 gcagctcgca aagactacac ttcagacaaa atctctgaaa acgaagctta ccaatacgca
    66361 gtcgttcgta acatccttta tcgtaaaggc tatgcaactg agatcttggt aaactatgag
    66421 ccatcacttc aatacttctc agaatggtgg aaacaattgg ctggtgaatc agaaggaaaa
    66481 gaccaaaaag gtatctaccc aacttcagcc aacttctcaa ctgacttgca ctcacttggt
    66541 caatttatcc aagaaggaac tcgtatcatg tttgaaacag ttgtccgtgt tgacaaacct
    66601 cgtaaaaacg tgcttattcc tactttggaa gaagaccttg acggacttgg ttaccttcaa
    66661 ggaaaagacg ttgactttgt aaacaaaaaa gcaactgacg gtgttcttct tgcccacaca
    66721 gatggtgatg taccaaacat gtatgtgact cttccagagc aagacgcttt cactcttggt
    66781 tacactatct acttcttcga attggcaatt gccctttcag gttacttgaa tgctatcaac
    66841 ccatttgacc aaccaggtgt tgaagcttat aaacgtaaca tgtttgccct tcttggaaaa
    66901 ccaggatttg aagaattgag caaagaactt aacgcacgtc tataatagaa gaaaagagtg
    66961 gtttgcccac tctttttact ctctttatcc atagaaattg gactcagcca agacttgtga
    67021 tataatatag aaagcaaaaa ggcagacgcc tagataatag gagaaactat gtcaaaagat
    67081 atccgcgtac gttacgcacc aagtccaaca ggactactac acatcggaaa tgcccgtaca
    67141 gcattgttta actacttgta tgcgcgccat catggtggaa catttctcat ccgtatcgaa
    67201 gatactgacc gtaaacgcca tgtcgaggat ggtgaacgtt cacaacttga aaaccttcgc
    67261 tggttaggca tggattggga tgaaagtcca gaatcacatg agaattatcg ccagtctgag
    67321 cgtttggact tgtatcaaaa atacatcgac caactattag ctgaaggaaa agcctacaaa
    67381 tcttacgtta cagaagaaga gttggcagct gaacgtgaac gtcaagaagt agctggcgaa
    67441 acaccacgct acatcaatga ataccttggt atgagtgaag aagaaaaagc agcttacatc
    67501 gcagaacgtg aagcagcagg gatcatccca actgttcgtt tggctgtcaa tgagtcaggt
    67561 atctacaagt ggcatgatat ggtcaaaggc gatatcgaat ttgaaggtgg caatatcggt
    67621 ggtgactggg ttatccaaaa gaaagacggt tacccaactt acaactttgc cgttgttatc
    67681 gatgaccacg atatgcaaat ctctcatgtt atccgtggag atgaccatat tgctaataca
    67741 ccaaaacagc ttatggtcta tgaagctctt ggttgggaag ctccagagtt cggtcacatg
    67801 accttgatta tcaactctga gactggtaag aagttatcta aacgtgatac taatacactt
    67861 caatttatcg aagattaccg aaagaaaggt taccttccag aagcagtctt taactttatt
    67921 gctcttcttg gttggaaccc aggtggtgaa gatgagattt tctctcgtga agaactcatt
    67981 aaactttttg atgaaaaccg cctcagcaag tcaccagcag cctttgatca gaaaaaacta
    68041 gactggatga gcaatgatta tatcaagaat gcagaccttg aaaccatctt tgaaatggca
    68101 aaaccattct tagaggaagc aggccgtttg actgacaagg ctgaaaaatt agttgagctc
    68161 tataaaccac aaatgaaatc agtagatgag attatcccat tgacagatct tttcttctca
    68221 gatttcccag aattgacaga agcagagcgc gaagtcatga cgggtgaaac agttccaaca
    68281 gttcttgaag cattcaaagc aaaacttgaa gcgatgacag atgataaatt tgtgacagaa
    68341 aatatcttcc cacaaattaa agcagttcaa aaagaaacag gtattaaagg gaaaaatctt
    68401 ttcatgccta ttcgtatcgc agtttcaggc gaaatgcatg ggccagaatt accagataca
    68461 attttcttgc ttggacgtga aaaatcaatt cagcatatcg aaaacatgct aaaagaaatc
    68521 tctaaataaa gaggatacaa taatggacat ctgggaaaag atgtacgaag aagcacagaa
    68581 actatacaat ccacatgaag tatcagactt tgtttatgcc aatcatgttg tagctgcagt
    68641 agaagcagaa gatggacaaa tatttacagg attctgtatg gagggaacct gtggtgtttt
    68701 ccatctctgc gcagaacggg cgaccctctt caatatgtac caattttcag gacaaactaa
    68761 ggttaagaaa gtattagcct ttcgagacaa accaccttat ggtggaagtt cagccatgcc
    68821 ttgcggtgct tgtagagaat tccttttaga gcttaatgct gaaaataaag atgcagaatt
    68881 catgatggac tataatataa gaaaaacagt taaagtcgca gaactaatcc cttattggtg
    68941 gggagaagaa cgtgcttcta agtttaatga gcgatagaag agtcagtata attctggctt
    69001 tttttactat aagttgaaaa aataaaaaaa attagaaaaa gtagttgaca aagtttgaaa
    69061 agactgtata atagtaagag ttgaaaataa caactcaggt ccgttggtca aggggttaag
    69121 acaccgcctt ttcacggcgg taacacgggt tcgaatcccg tacggactat ggtatgttgc
    69181 gtcaggacca cttgatgaaa aaaagtttaa aaaaacttaa aaatcttcaa aaaagtgttg
    69241 acaagtgaaa gtgg
//
LOCUS       DAVKMU010000004        60228 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKMU010000004 DAVKMU010000000
VERSION     DAVKMU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 60228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60228
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.004"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..137)
                     /locus_tag="VW343_000316"
     CDS             complement(<1..137)
                     /locus_tag="VW343_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9754934.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     G"
     gene            313..1758
                     /gene="cps4A"
                     /locus_tag="VW343_000317"
     CDS             313..1758
                     /gene="cps4A"
                     /locus_tag="VW343_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9754935.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVITALVLLVALLGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAVYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNMPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1760..2491
                     /locus_tag="VW343_000318"
     CDS             1760..2491
                     /locus_tag="VW343_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEU9754936.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKVKELFVDNPRKIIMDQLI"
     gene            2500..3192
                     /gene="cpsC"
                     /locus_tag="VW343_000319"
     CDS             2500..3192
                     /gene="cpsC"
                     /locus_tag="VW343_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU9754937.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSMTRIYVVNRNQGEKSGLTNQDLQAGSSLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKDLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPATSPSSPNIKRSTLIGFLAGVIGTSVIVLILELLDTRVKRPKDIEDTLHMT
                     LLGIVPNLNKLK"
     gene            3202..3891
                     /gene="cpsD"
                     /locus_tag="VW343_000320"
     CDS             3202..3891
                     /gene="cpsD"
                     /locus_tag="VW343_000320"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEU9754938.1"
                     /translation="MPTLEIAQKKLEFVKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PEEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGTVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEVNKRDVQKAKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GFYGNYGKKII"
     gene            3902..5269
                     /locus_tag="VW343_000321"
     CDS             3902..5269
                     /locus_tag="VW343_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9754939.1"
                     /translation="MDEKGLKIFLAVLQSIIVILLVYFLSFVRETELERSSMVILYLL
                     HFFVFYFSSYGNNFFKRGYLVEFNSTIRYIFFFAIAISVLNFFIAERFSISRRGMVYF
                     LTLEGISLYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKL
                     VAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETMG
                     IDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVIAKRIIDIMGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYVQYTSE
                     QKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            5273..6016
                     /locus_tag="VW343_000322"
     CDS             5273..6016
                     /locus_tag="VW343_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU9754940.1"
                     /translation="MRDRIQLLGVTIDLLTMNETIDIVEQYVLEKRPLHLMGVNADKI
                     NQCHTDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEVLQDMLKVFKRDYPNLIVIGHRNGYFSEEDEQAIQEDIREKNPDFVF
                     IGITSPKKEYIIQKFMDSGVNSVFMGVGGSFDVLSGHIQRAPLWMQKSNLEWLFRVAN
                     EPKRLFKRYFVGNISFIGRVLKAKRGVKY"
     gene            6013..6837
                     /locus_tag="VW343_000323"
     CDS             6013..6837
                     /locus_tag="VW343_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU9754941.1"
                     /translation="MNQTEMIRLIQKVELDAIKEFKKICEENDIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREAYDKLPSVFKDRIIAGKYQVLTYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSVLRKIYFGKVYWYRFLASLGTTYVGDH
                     VDMHSTKQKLIIGFFKKLGFAKLFPQNSVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDTFFKVPTEYDRYLKRLYGENYLYEEPSDDEKKSHLGGH"
     gene            6839..7717
                     /locus_tag="VW343_000324"
     CDS             6839..7717
                     /locus_tag="VW343_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9754942.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNYNAKQIVIIDNFSNNGTG
                     EKLQELYESDLEIDVLINHENAGFARGNNVAYQFAKEKYNPDFMVIMNNDIEIETENF
                     EKIVTDIYREEKFHLLGPDIFSTTYQLHQNPKRLTHYTYGEVKALNEKFKKGSQVSLA
                     LKIKCWLKASKVLRTAIYQNRRKKGSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIRVLHHQNVATNQVYTNLLEKTLFSNKCNF
                     KSTSYFLKLMKENEDV"
     gene            7718..9055
                     /locus_tag="VW343_000325"
     CDS             7718..9055
                     /locus_tag="VW343_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU9754943.1"
                     /translation="MSYLFLLCLTLFLLTIFYFFAFIQDLIAPPVVMSVMFLISSVFA
                     LVNSKNWNIEYSGIAYILIISGIIIFSLPLMALKSPNFNTEVKIADRLIDIQFWKIAL
                     TIIIDLFILYLYRKEIYNLVLSNGYTGSNIQWFFRNATSYEGELTVRTFIRVLIRVID
                     VSAYIFGYTFINNFLIYRHKRPKDILLLVPLLIFISKTLISGGRQDIIKILIAYVIMM
                     YIQQKRKVGWNRVISHKYIHLGFVGLIAGIPAFYYSLFLAGRSTTRTLFESVSTYLGG
                     SIQHFNQYIENPLDPGEVFGSETLVPILNILGEMGLVNYRSTIHLEFRTLGVTVGNVY
                     TFFRRPLHDFGLVGMYVFVFAVGAFFAIYYLVLRKKQVGFNLDIHTIIYSYVFYWIFL
                     SSIEQYSFTMISLYTLVFIVLVYFMAIFYWCTDFKRGKLIFKISDSSIKLKEE"
     gene            9078..10499
                     /locus_tag="VW343_000326"
     CDS             9078..10499
                     /locus_tag="VW343_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754944.1"
                     /translation="MNTKIKNIITSFSYVISSNLLIVLTSSLVVLIFPKIMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYTNLDRKQFYSQMILFSSFLMLISLVLF
                     TLNLITVRDENARYIYNMAIISMIVTNLRTLYVYILQMTNRLKDSSVILISDRVLYVL
                     LLFMFIVFGWHEYKVMIWADILGRTFSLMLSFWICKDIVFQPLSKFILDFKESLDNIR
                     VGINLMLSNIASSMIIGIVRMGIQWNWNIETFGKVSLTLSISNLLMTFINAIGLVIFP
                     LIKRTKTENLPKIYSNLRNALMLVMFAILLFYYPLKFILDIWLPAYKDALVFMALIFP
                     MSVYEGKMALVINTYLKAMRMEKDILKINALVMLTSMVVTLVTTLLLNNLGLTVVSIV
                     ILLALRSIIAELILSKKLKISVKQDIALELLMTIIFISSSWYLSIWIAVIIYLLAYTL
                     YLYLKHKDIRMYIEYFKNHKKIS"
     gene            10563..11651
                     /gene="wecB"
                     /locus_tag="VW343_000327"
     CDS             10563..11651
                     /gene="wecB"
                     /locus_tag="VW343_000327"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU9754945.1"
                     /translation="MKKIMLVFGTRPEAIKMCSLVNELRKQEDMKTVVCVTGQHKEMV
                     SPVLDLFGVQPDYDLEIMKANQNLFSITISILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIANYHFAPTELAKENLIKE
                     GRNNIYVTGNTVIDALTTTVQKDYTHPDLDLNDGNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYDDVKVIYPIHKNPLVRETATEIFGDTERIQIIEPLDVLDFHNFMNHSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKMLLDDPE
                     EYKKMSQASNPYGNGDASKQIVRILRGI"
     gene            11690..12559
                     /gene="rfbA"
                     /locus_tag="VW343_000328"
     CDS             11690..12559
                     /gene="rfbA"
                     /locus_tag="VW343_000328"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU9754946.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            12560..13156
                     /locus_tag="VW343_000329"
     CDS             12560..13156
                     /locus_tag="VW343_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU9754947.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            13166..14215
                     /gene="rfbB"
                     /locus_tag="VW343_000330"
     CDS             13166..14215
                     /gene="rfbB"
                     /locus_tag="VW343_000330"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU9754948.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            14281..15132
                     /gene="rfbD"
                     /locus_tag="VW343_000331"
     CDS             14281..15132
                     /gene="rfbD"
                     /locus_tag="VW343_000331"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU9754949.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVARASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            15208..15642
                     /locus_tag="VW343_000332"
     CDS             15208..15642
                     /locus_tag="VW343_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9754950.1"
                     /translation="MLLVLVSLAHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTF
                     LSGKAENQVITLYHHDQCRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            15715..15972
                     /locus_tag="VW343_000333"
     CDS             15715..15972
                     /locus_tag="VW343_000333"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9754951.1"
                     /translation="MVNCEPLEAYRQLEEAELVGCWAHVRRKFFEVPPKQADKSSLGA
                     KGLAYCDQLFSLERDWEALPADERLQKRQEHLQPLMEDFFA"
     gene            16018..16164
                     /locus_tag="VW343_000334"
     CDS             16018..16164
                     /locus_tag="VW343_000334"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9754952.1"
                     /translation="MEYSLKYEETFKTILKDGHLVLSNNLAERAIKSLVMGRSKRVQW
                     TLLA"
     gene            16235..16393
                     /locus_tag="VW343_000335"
     CDS             16235..16393
                     /locus_tag="VW343_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9754953.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            16749..17225
                     /locus_tag="VW343_000336"
     CDS             16749..17225
                     /locus_tag="VW343_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9754954.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            17263..17568
                     /locus_tag="VW343_000337"
     CDS             17263..17568
                     /locus_tag="VW343_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9754955.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            17616..19091
                     /locus_tag="VW343_000338"
     CDS             17616..19091
                     /locus_tag="VW343_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9754956.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            19117..19194
                     /locus_tag="VW343_000339"
                     /pseudo
     CDS             19117..19194
                     /locus_tag="VW343_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            19396..26097
                     /locus_tag="VW343_000340"
     CDS             19396..26097
                     /locus_tag="VW343_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9754957.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIENRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLYKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVKVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKDQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     VPKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSFPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKIEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPSTGSAASQAAVAAGLTLLGLSAGLVVTKGKKED"
     gene            complement(26345..27201)
                     /locus_tag="VW343_000341"
                     /pseudo
     CDS             complement(26345..27201)
                     /locus_tag="VW343_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27411..27770)
                     /locus_tag="VW343_000342"
     CDS             complement(27411..27770)
                     /locus_tag="VW343_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9754958.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            27793..28371
                     /locus_tag="VW343_000343"
     CDS             27793..28371
                     /locus_tag="VW343_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846832.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEU9754959.1"
                     /translation="METRNRGIYRKVRLLRLWVIFVIMKVMKSYNTLNDYYRKLFGEK
                     TFKVPIDAGFDCPNRDGTVAHGGCTFCTVSGSGDAIVAPDAPIREQFYKEIDFMHRKW
                     PDVQKYLVYFQNFTNTHEKVEVVRERYEQAINEPGVVGINIGTRPDCLPDETIEYLAE
                     LSECMHVTVELGLQTTYEATSDLINRAHSYEL"
     gene            28372..28818
                     /locus_tag="VW343_000344"
     CDS             28372..28818
                     /locus_tag="VW343_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU9754960.1"
                     /translation="METVKRLRKYPKIEIVSHLINGLPGETHEMMVENVRRCVTNNDI
                     QGIKLHLLHLMTNTRMQRDYHEGRLQLMSQDEYVRVICDQLEIIPKHIVIHRITGDAP
                     RDMLLGLMWSLNKWEALNSIEMEMRRRGSVQGCKAVKQEFENEKTT"
     gene            28802..29359
                     /locus_tag="VW343_000345"
     CDS             28802..29359
                     /locus_tag="VW343_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9754961.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            29352..29606
                     /locus_tag="VW343_000346"
     CDS             29352..29606
                     /locus_tag="VW343_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754962.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            29618..31672
                     /locus_tag="VW343_000347"
     CDS             29618..31672
                     /locus_tag="VW343_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9754963.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            31851..32711
                     /locus_tag="VW343_000348"
     CDS             31851..32711
                     /locus_tag="VW343_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9754964.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            32922..33632
                     /gene="ccdA2"
                     /locus_tag="VW343_000349"
     CDS             32922..33632
                     /gene="ccdA2"
                     /locus_tag="VW343_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9754965.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            33652..34218
                     /locus_tag="VW343_000350"
     CDS             33652..34218
                     /locus_tag="VW343_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9754966.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            34229..35341
                     /gene="msrB"
                     /locus_tag="VW343_000351"
     CDS             34229..35341
                     /gene="msrB"
                     /locus_tag="VW343_000351"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9754967.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            35699..36436
                     /locus_tag="VW343_000352"
     CDS             35699..36436
                     /locus_tag="VW343_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9754968.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            36433..38124
                     /locus_tag="VW343_000353"
     CDS             36433..38124
                     /locus_tag="VW343_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9754969.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            38240..38803
                     /locus_tag="VW343_000354"
     CDS             38240..38803
                     /locus_tag="VW343_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9754970.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            38825..44767
                     /locus_tag="VW343_000355"
     CDS             38825..44767
                     /locus_tag="VW343_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU9754971.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAEAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEKAPVEPEKQPEAPEEEKAVEETPKQEDTQPEVVETKDEAANQP
                     VEEPKVETPAVEKQTEPTEEPKVEQVGEPVEPREDEKAPVSPEKQPEAPEEEKTAEET
                     PKQEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLETAKGVYASE
                     PVKQPEVNSETKAEKVAANTDAKQSEVNSETASLKTAISGLNTDKVELENQLKIAQGK
                     TETDFSMESWTVLSTAKNKAQEVKDNGTATQEQINEAEKSLKTALADLSVDKTALGSA
                     IDTATKKNKENYTNQTWAELETVLTAAKSVNTNESKQSEVNEAVEKLTATIEKLVELS
                     EKPRLTLSIEKRDIDRKVTVTYTLENPANTQIKSITATLKKGEEVVKDFVLTEENLKT
                     NHLTALFEKLDYYKEYTLSTDMVYNRGNDDETESISEELIQLNLKKLELKDIQTVSLM
                     KFENGQESQVTHLSDKPTDLSKLYLKVTSSTSKDAVLAVSSIEEEIVENKKIFKIHAD
                     TPELVVRKKDGSLSKGFDYYMERVIPHDGDIYYDFKDLISAMTSNPTGTFILGRDISS
                     RNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIFDLEYPLFDTIKSGVVKDIDFKHVNM
                     VFPDSNQGDNVATIARVIKDKTKIENVNVEGYLEGRDHVAGLVNNLEGNSEIENISFT
                     GKIKSKGGNSITAGIAGRNILSRVKRAYVNANIEVLGSTNSSMLVAVNGTTLNASGGW
                     GAWGRLTESVAKGTLEIKRSGQAGGVTATVWPYGAIDKVVSYAKVTKGKELFGSDGDL
                     NNNWFMQKINNIFGVQGISSGDSGNDSKFKRISEEEAKQKVASYNITAPNLMSDSSLL
                     VDRLNESWKNTDQFESIQDYQSQNQLIYQNLTKFTPYYNKEFIVHEGNALTPEQEILK
                     TKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGSQKRYKVTSTGKFSITNLPEYQVEDL
                     NVVYTSEHIVHPLDSSLINNLVEELKKVELYTESTYQVLGIDKDNANKLNRTKRLFLD
                     ESLDAVKTQLPTFVKTMFENEWLHINGESSGAVAALRQKIMDNKTAILLALTYINRYY
                     DVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRLGSSGENRLKGSENAETFKQLFASET
                     KQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDYIQFEERPSLVEEIKDAKYRVYDNLT
                     APYYQGYILPLLTLKNTHLAILSNYSTMTFVSREKRPNWKNEDFDKWVKYVATAHRNH
                     VDTWYKILPDNIKGKMVKENVTAVWEGLSIPGSEWVDQNAVDRKGRDYAPAREFFNLV
                     GGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSEYGISVFTHELTHVNDTWIYLGGYGR
                     RENMGPEAYAQGLFQSPVPGQPGWGALGLNMAFERKNDGDLIYNASPTQFENRKELDS
                     YMKNYNDTLMMVDYLEGDAVISKGKEAITKWFKKVEPKVVSQTAQYDTVRQLTAEEKE
                     KLSVSSVDDLVDQGLMSDRAVGNNTYNPADFETSYIAIDYMTGIYGGGKNSVGSPGAL
                     MFKHNTFRMWGYYGFEEGVLGYASNKFKQASRDEGHAGLSDNFIISKISKGEFLTMEA
                     FKKGYFKKVVEELKTKGIRPVTINQKTYSTFEELQEGFKQAVERDLKKNQLDERETRN
                     FKFQVFRQLLQQTDSFKTSIFR"
     gene            44828..46549
                     /gene="pabB"
                     /locus_tag="VW343_000356"
     CDS             44828..46549
                     /gene="pabB"
                     /locus_tag="VW343_000356"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9754972.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            46550..47167
                     /locus_tag="VW343_000357"
     CDS             46550..47167
                     /locus_tag="VW343_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9754973.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SFMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            47217..48215
                     /locus_tag="VW343_000358"
     CDS             47217..48215
                     /locus_tag="VW343_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9754974.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEREPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTV
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            48342..49301
                     /locus_tag="VW343_000359"
     CDS             48342..49301
                     /locus_tag="VW343_000359"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9754975.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            49398..50237
                     /locus_tag="VW343_000360"
     CDS             49398..50237
                     /locus_tag="VW343_000360"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9754976.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRIDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(50285..50455)
                     /locus_tag="VW343_000361"
     CDS             complement(50285..50455)
                     /locus_tag="VW343_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9754977.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            50576..51460
                     /gene="miaA"
                     /locus_tag="VW343_000362"
     CDS             50576..51460
                     /gene="miaA"
                     /locus_tag="VW343_000362"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9754978.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            51453..52691
                     /gene="hflX"
                     /locus_tag="VW343_000363"
     CDS             51453..52691
                     /gene="hflX"
                     /locus_tag="VW343_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9754979.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            52684..53307
                     /locus_tag="VW343_000364"
     CDS             52684..53307
                     /locus_tag="VW343_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9754980.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            53322..54251
                     /locus_tag="VW343_000365"
     CDS             53322..54251
                     /locus_tag="VW343_000365"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9754981.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            54272..55027
                     /locus_tag="VW343_000366"
     CDS             54272..55027
                     /locus_tag="VW343_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9754982.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(55081..55989)
                     /locus_tag="VW343_000367"
     CDS             complement(55081..55989)
                     /locus_tag="VW343_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9754983.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            56053..56322
                     /locus_tag="VW343_000368"
     CDS             56053..56322
                     /locus_tag="VW343_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9754984.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            56322..56702
                     /locus_tag="VW343_000369"
     CDS             56322..56702
                     /locus_tag="VW343_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9754985.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            56820..57017
                     /locus_tag="VW343_000370"
     CDS             56820..57017
                     /locus_tag="VW343_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9754986.1"
                     /translation="MGILSIILGLLFPIVGLILGIIGLVLAISYQKESQLDYKIEKIL
                     NILGIVISVVNWIVAIALIFR"
     gene            complement(57057..57782)
                     /locus_tag="VW343_000371"
     CDS             complement(57057..57782)
                     /locus_tag="VW343_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9754987.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            57944..59785
                     /gene="typA"
                     /locus_tag="VW343_000372"
     CDS             57944..59785
                     /gene="typA"
                     /locus_tag="VW343_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9754988.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPVEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            59805..60059
                     /locus_tag="VW343_000373"
     CDS             59805..60059
                     /locus_tag="VW343_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9754989.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT        18676 a        10622 c        13142 g        17788 t
ORIGIN      
        1 tttcctttct gttgataggc tagtagcaga cgattgattt gtctttcaga aagattgagt
       61 tcgacacagg cccgtttctt tgttttcttt ccttgggcta tagcttttat cacaagatat
      121 tttttcgttt cattcatatt cagttggatc cttttcatat gactattcta ccaaatggga
      181 cattttcacg ttcgatttac taaagacatt atcacattcg aattacatta aagatgcaga
      241 tagtaaaaaa atgtagacat taccgtaaaa aagtgatata atcgtatgat gttcaaggta
      301 taggtgttaa tcatgagtag acgttttaaa aaatcacgtt cacagaaagt gaagcgaagt
      361 gttaatatcg ttttgctgac tatttattta ttgttagttt gttttttatt gttcttaatc
      421 tttaagtaca atatccttgc ttttagatat cttaacctag tgataactgc gttagtccta
      481 ctagttgcct tgctagggct actcttgatt atctataaaa aagctgaaaa gtttactatt
      541 tttctgttgg tgttctctat ccttgtcagc tctgtgtcgc tctttgcagt acagcagttt
      601 gttggactga ctaatcgttt aaatgcgact tctaattact cagaatattc aatcagtgtc
      661 gctgttttag cagatagtga tatcgaaaat gttacgcaac tgacgagtgt gacagcaccg
      721 actgggactg ataatgaaaa tattcaaaaa ctactagctg atattaagtc aagtcagaat
      781 accgatttga cggtcaacca gagttcgtct tacttggcag tttacaagag tttgattgca
      841 ggggagacta aggccattgt cctaaatagt gtctttgaaa acatcatcga gtcagagtat
      901 ccagactacg catcgaagat aaaaaagatt tataccaagg gattcactaa aaaagtagaa
      961 gctcctaaga cgtctaagaa tcagtctttc aatatctatg ttagtggaat tgacacctat
     1021 ggtcctatta gttcggtgtc gcgatcagat gtcaatatcc tgatgactgt caatcgagat
     1081 accaagaaaa tcctcttgac cacaacgcca cgtgatgcct atgtaccaat cgcagatggt
     1141 ggaaataatc aaaaagataa attaacccat gcaggcattt atggagttga ttcgtccatt
     1201 cacaccttag aaaatctcta tggagtggat atcaattact atgtgcgatt gaacttcact
     1261 tctttcttga aaatgattga cttattggga ggggtagatg ttcataatga tcaagagttt
     1321 tcagctctac atgggaagtt ccatttccca gtagggaatg tccacctaga ctctgagcag
     1381 gctctaggtt ttgtacgtga acgctactca ctagccgatg gagaccgtga tcgtggtcgc
     1441 aaccaacaaa aggtcattgt agcaattatt cagaagttaa cttctacaga ggttttgaaa
     1501 aactatagta gtattcttca aggattgcag gattctcttc aaacaaatat gccgattgag
     1561 actatgatag atttagtgaa tactcagttg gaaagtgggg ggaattataa agtaaattct
     1621 caagatttaa aagggacagg tcggatggat cttccttctt atgcaatgcc agacagtaac
     1681 ctctatgtga tggaaataga tgatagtagt ttagctgtag ttaaagcagc tatacaggat
     1741 gtgatggagg gtagatgaaa tgatagacat ccattcgcat atcgtttttg atgtagatga
     1801 cggtcccaag tcaagagagg aaagcaaggc tctcttggca gaatcctaca ggcagggggt
     1861 gcgaaccatt gtctctacct ctcaccgtcg caagggcatg tttgaaactc cggaagagaa
     1921 gatagcagaa aactttcttc aggttcggga aattgcaaaa gaagtggcag atgatttagt
     1981 cattgcttat ggcgcagaga tatactatac tctggatgct ctagaaaagc tagaaaaaaa
     2041 agaaattcct acccttaatg atagtcgtta tgccttgatt gagtttagca tgcatacttc
     2101 ctatcgtcag attcatacgg gattgagcaa tattttgatg ttgggaatca cgccagtaat
     2161 tgctcatatt gaacgttatg atgctttaga gaataacgaa aaacgtgttc gtgaactgat
     2221 tgatatgggg tgctatactc agataaatag ttatcatgtt tcaaaaccta agttctttgg
     2281 tgaaaaatat aaattcatga aaaagagagc tcggtatttt ttggaacgtg atttagttca
     2341 tgtagttgca agtgacatgc acaatttaga cagtagacct ccatatatgc aacaggcata
     2401 tgatatcatt gctaagaaat atggagcgaa aaaagtaaaa gaactttttg tagataatcc
     2461 cagaaaaatt ataatggatc aattaattta ggagaaaata tgaaggaaca aaacactttg
     2521 gaaatcgatg tattgcaact attcagagct ttatggaaaa gaaagttggt cattttatta
     2581 gtggcaatta taacttcttc agttgctttt gcctacagta cttttgttat caaacctgag
     2641 tttactagta tgactcggat ttatgtagtt aaccgtaatc agggagagaa gtctggttta
     2701 accaatcaag acttgcaggc aggatcatcc ttggttaaag actatcgtga aattatccta
     2761 tcgcaggatg ttttggagga agttgtttct gatttgaaac tagatttgac gccaaaagat
     2821 ttggctaata aaattaaagt aacagtacca gttgataccc gtattgtctc tgtttcagtt
     2881 agtgatcgag ttcctgaaga ggcaagccgt atcgctaact ctttgagaga agtagctgct
     2941 caaaaaatta tcagtattac tcgtgtttct gatgtgacaa cactggagga ggcaagaccg
     3001 gcgacatcac cgtcttcgcc aaatattaaa cgcagtacac taattggttt tttggcagga
     3061 gtgattggaa ctagtgttat agttcttatt cttgaacttt tggacactcg tgtgaaacgt
     3121 ccgaaagata tcgaagatac actgcatatg acacttttgg gaattgtacc aaacttgaat
     3181 aagttgaaat aggagagagg aatgccgaca ttagaaatag cacaaaaaaa actggagttc
     3241 gttaagaagg cagaagaata ttacaatgcc ttgtgtacaa atatacagtt gagcggagat
     3301 aaactaaaag taatttccgt tacttctgtt aaccctgagg aaggaaaaac aactacttcc
     3361 gtaaatatag caaggtcgtt tgcgcgtgca ggctataaaa ctcttttgat cgatggcgat
     3421 actcgaaatt cagttatgtc aggatttttt aaatctcgtg aaaaaattac agggctaacg
     3481 gaatttttat ctgggacagc tgatttatct cacggtttat gtgatacaaa tattgaaaat
     3541 ttatttgtag ttcaatcggg cactgtatca ccaaacccta cagccttgtt acaaagtaaa
     3601 aattttaatg atatgattga aacattgcgt aaatattttg attatatcat tgttgataca
     3661 gcacctattg gaattgttat tgatgcggca attatcactc aaaagtgtga tgcgtccatc
     3721 ttggtaacag caacaggtga ggtgaataaa cgtgatgtcc aaaaagcgaa acaacaatta
     3781 gaacaaacag ggaaactgtt cctaggagtt gttttgaata aattagatat ctcggttgat
     3841 aagtatggag tttacggttt ctatggaaat tatggtaaaa aaataattta ggaaagattc
     3901 tatggatgaa aaaggattga aaattttttt ggcagtatta cagagtatta ttgtcatttt
     3961 attggtttat tttcttagct ttgttagaga gacagaactt gaacgttctt cgatggttat
     4021 actatacctt ctccactttt ttgtattcta ttttagttcc tatggtaaca atttttttaa
     4081 aagagggtac ctagttgagt ttaatagtac cataagatat atttttttct ttgcaatagc
     4141 tataagtgta ttaaactttt ttatagcgga acggtttagt atctctagaa gaggaatggt
     4201 atacttctta actttagaag gaatatcctt atacttgtta aatttcttag taaagaaata
     4261 ttggaagcat gtgtttttta atctaaaaaa tagcaagaaa attttactgt taacagtaac
     4321 gaaaaatatg gaaaaagttc ttgataaatt gctagaatct gatgaacttt catggaaatt
     4381 ggtagcagta agtgttttgg ataaatctga ttttcaacat gataaaatac ctgtaattga
     4441 aaaggaaaaa attattgaat ttgcaacgca tgaagttgtg gatgaggtgt ttgtcaatct
     4501 tccaggagag agctacgata ttggagaaat tatctctagg tttgagacaa tggggataga
     4561 tgtaactgta aatcttaaag catttgataa gaatttgggt cgcaataaac aaattcatga
     4621 gatggtagga ttgaatgtag tcactttctc tacaaatttt tataaaacta gtcatgtaat
     4681 tgctaagcgg attattgata tcatgggtgc attggtaggg ctgatactat gtggtttagt
     4741 cagtattgta ttggttcctt tgattcgaaa ggatgggggc tctgctattt ttgctcagac
     4801 gcgtatagga aaaaatggtc gtcagttcac tttttataag tttcgctcta tgtgtgtaga
     4861 tgccgaggcg aaaaaaagag aactcatgga acaaaatacc atgcagggtg gaatgtttaa
     4921 ggtggacgat gatcctcgta tcacgaaaat tggtcgtttt atacggaaga ctagcttgga
     4981 cgagttacca cagttttata atgttctaaa gggagatatg agtttggttg gcacacggcc
     5041 acctacagtg gacgagtatg ttcagtatac ttcagaacag aaacgtcgac tcagctttaa
     5101 acctggtatt acaggtttat ggcaggttag cggccgtagt aaaataaccg attttgacga
     5161 tgttgtaaaa ttagatgtgg cttatattga taattggaca atctggaaag atattgaaat
     5221 tttgcttaaa actgttaaag ttgtatttat gagagatgga gcgaagtgag gaatgaggga
     5281 tagaatccaa cttttaggtg taacaattga tttgcttacg atgaatgaaa cgatagatat
     5341 tgtagaacaa tatgtattag aaaaaagacc actacacttg atgggggtga atgctgataa
     5401 aattaatcag tgtcatacag atgagaaaat caaaaaaatc gttaatgagt caggaatcat
     5461 taatgcggat ggagcatcag ttgttcttgc aagtaagttt ttaggaacgc ctgttcctga
     5521 acgagtagcg ggtattgatt tgatgcaatg tcttttagag ttgtcaaata aaaaaggata
     5581 ttcagtttac ttttttggag caaaagaaga agttttgcaa gatatgctca aagtatttaa
     5641 gagagattat ccaaatttga tagttattgg acacagaaat ggctattttt ctgaagagga
     5701 tgaacaagct attcaagaag atattcgtga aaagaaccct gattttgtgt ttattggaat
     5761 tacgtctcct aaaaaagaat atattattca aaaatttatg gatagtggcg tcaattcggt
     5821 atttatggga gttggcggta gttttgatgt cttgtctggt catatccaac gagcacctct
     5881 atggatgcaa aagtcaaatt tagagtggtt attccgtgta gctaatgagc ctaaacgtct
     5941 ctttaaacgt tattttgtag ggaatatttc attcatagga agagttttaa aagcaaaaag
     6001 aggtgtaaaa tattgaacca gacagagatg attcgcttaa ttcaaaaagt tgaattagat
     6061 gctataaaag agtttaaaaa aatctgtgaa gagaatgata tagatttttt cctccgcggt
     6121 ggtagtgtac ttggtgcggt caaatacgac ggctttattc catgggatga tgatatggat
     6181 atcgctgtcc ctcgtgaagc atacgacaaa cttccaagtg ttttcaaaga tagaattatc
     6241 gctgggaaat atcaggttct tacttatcaa tactgtgata cgttgcattg ctactttcct
     6301 cgactattcc ttttagaaga tgaaagaaaa cgtttgggct tgccacgaaa taccaatcta
     6361 ggattgcatt tgattgatat cattccttta gatggagcac caaatcattc ggttttaaga
     6421 aagatttact ttggcaaagt atactggtat cgttttttag caagtttagg aacaacttat
     6481 gttggcgacc atgtggatat gcattccact aagcaaaaac taattattgg tttctttaaa
     6541 aaactaggat ttgcaaaact atttcctcaa aattctgtat acagacgctt ggataatctc
     6601 tataaaaagt atgattggaa aaagcagaag tatgcgggga ctatcaatgc ttctttattt
     6661 gctaaagaag ttatgccagt agagatttgg ggagaaggag tagagaagcc ttttgaggat
     6721 actttcttta aagttccaac ggagtatgat cgctacctga aaagacttta cggagaaaac
     6781 tatctttacg aagagcctag tgatgatgaa aagaaatcgc atttaggagg acactaattt
     6841 gttttgttat attattttgc attacaaagt cttagaagaa actatttctt gtgttaaatc
     6901 tataaaagaa ggcaattata atgcaaagca aatcgttatt attgataatt tttctaataa
     6961 tggtactggt gaaaaactac aagagcttta tgaatcagat ttagaaattg atgttttgat
     7021 taaccatgaa aatgctggtt ttgctcgtgg aaataatgtg gcttatcaat ttgctaagga
     7081 aaagtataac cccgatttca tggttatcat gaataacgat attgagatag aaacagaaaa
     7141 ttttgaaaaa attgtgacag atatctatcg tgaggaaaaa ttccatttgc tcgggccaga
     7201 tatcttctcg actacttacc aacttcacca aaacccaaaa cggttgacac attatactta
     7261 tggagaagtt aaagctctaa atgaaaaatt taaaaaaggg agccaagtta gtctagcttt
     7321 aaaaatcaaa tgttggttga aagctagtaa agttcttcga acagcaatct atcaaaatag
     7381 acgtaaaaaa ggatcagtag actatagaaa acaggtagaa aacccaattc ttcatggttc
     7441 ttttattgta tattcgagag attttatcga aaaagaggag tatgctttta accctaacac
     7501 cttcttctac tatgaaacag agatattaga ttatgaagct gaattaaaag gatacaagag
     7561 aatttataca cctaaaatta gagttttgca ccatcaaaat gttgcaacta atcaagttta
     7621 cacgaacttg ttagaaaaaa ccttgttttc aaataaatgc aactttaaat ccaccagtta
     7681 ttttttgaag ttgatgaaag aaaacgagga tgtttaaatg agttatttat ttttactttg
     7741 ccttacatta ttcttattga ctatattcta tttctttgct tttattcaag atttaattgc
     7801 tcctccagta gttatgtctg taatgtttct aattagttca gtatttgcac tggttaattc
     7861 aaaaaactgg aatattgaat atagtggaat agcctatatt ctcataatta gtggtattat
     7921 tatattttcg cttcctttaa tggcattaaa atcacctaat tttaatactg aggttaagat
     7981 tgctgatcga ttaattgata tccaattttg gaaaattgct ctaactatta taattgatct
     8041 ctttattttg tatctttaca ggaaggaaat atataacctt gttcttagta atggatatac
     8101 ggggtcaaat attcagtggt tttttagaaa tgcaacgagt tatgaaggtg aattgacagt
     8161 gcgaactttt attcgagttc tcattcgtgt tattgacgta tctgcttata tttttggata
     8221 tacttttatt aataattttc ttatctatcg ccataaacgc cctaaagaca tattactttt
     8281 agtaccttta ttaatattta tttcaaaaac tttaatatca ggaggccggc aagatattat
     8341 taaaattctg attgcctatg taatcatgat gtatatccaa caaaaacgga aagttggatg
     8401 gaatagagtc atatctcata aatatattca ccttggattt gttggtttaa tagcaggtat
     8461 tccagcattt tactactctt tgtttttagc cggtcgttca acgactagga cgctatttga
     8521 gagtgtttcg acctatctag gaggctcaat tcagcatttt aatcagtata ttgaaaatcc
     8581 attagatcct ggtgaagttt ttggcagtga aacattggtg cctatattaa atatattagg
     8641 ggaaatgggc ctagttaatt atcgtagtac aattcattta gaatttcgga cactaggagt
     8701 tactgtagga aatgtttata ctttttttag aagacccttg catgattttg gtctagttgg
     8761 tatgtatgtg tttgtctttg ctgtaggtgc tttttttgct atttattatt tagttctgag
     8821 aaagaaacag gttggtttta atttggatat tcataccatt atttattctt atgtctttta
     8881 ttggattttt ttatcatcaa tcgagcaata ctcgttcaca atgattagtc tatatacact
     8941 tgtatttatt gtgttggttt actttatggc tatcttttac tggtgtacag attttaaaag
     9001 aggaaaactg atttttaaaa tttctgactc aagtatcaaa ttaaaagaag aataacagaa
     9061 tgtataggag agggtagatg aatactaaaa ttaaaaatat aataactagt ttttcttatg
     9121 ttatttcttc aaatctgctc atagttttaa cctcatcact agttgttttg atttttccta
     9181 aaataatggg ggtaactgag tacagttact ggcaacttta tattttttat ctgacctata
     9241 tcggtttttt ccacttgggt tggattgatg ggatttatct caaatatggt ggcttagaat
     9301 atacaaattt agatagaaaa cagttttatt ctcagatgat tctattttct agtttcttaa
     9361 tgctaatctc gctggtatta tttactttga acctaataac tgtaagggat gaaaacgcaa
     9421 gatatattta taatatggct atcattagca tgatagtcac aaacttaaga acactctatg
     9481 tttatatctt gcagatgaca aatcgcttga aggatagttc agtcattcta attagtgatc
     9541 gcgttttata tgtactcctt ttattcatgt ttattgtatt tggatggcat gagtacaagg
     9601 tcatgatttg ggctgatatt ctaggtcgaa cattttctct catgctttcc ttctggattt
     9661 gtaaagatat tgtgtttcag cctttgtcaa aatttatctt agatttcaag gagtcccttg
     9721 ataatatccg tgttggaatc aatttaatgc tatctaacat tgcgagtagc atgattatag
     9781 gcattgttcg tatgggaatt caatggaatt ggaatattga aacattcggg aaagtgtcat
     9841 taactttgag tatatctaat ttattaatga cttttattaa tgccattgga ttagttatct
     9901 ttcctttgat aaagcgaaca aagactgaga atttacctaa aatttattct aatttaagaa
     9961 atgctttgat gttggttatg ttcgcaatct tgctcttcta ctatccttta aaatttattc
    10021 ttgatatttg gcttcctgct tataaggatg ccttagtttt catggcccta atttttccta
    10081 tgtcagttta tgaagggaaa atggctttgg tgataaatac atatttaaaa gcaatgagaa
    10141 tggaaaaaga cattctcaaa attaatgctt tggttatgtt aactagtatg gtagtgacat
    10201 tagtgactac tctactacta aataatttgg ggctgacagt tgtatctata gttattttac
    10261 ttgctttaag aagtataata gctgaattaa ttttatccaa aaaactgaag atatcagtca
    10321 agcaagacat tgctttagag ttacttatga cgattatatt tatttcttca agttggtatc
    10381 tctctatttg gattgcagta ataatttatt tattggcgta tactttatat ttgtatttaa
    10441 agcacaaaga tatcagaatg tatatagaat actttaaaaa tcataaaaaa atatcataaa
    10501 aattatatat caatgaaatg gtagattaca tttctacctt tttatccatt taggaggaaa
    10561 cgatgaaaaa gataatgcta gttttcggta cacgtccaga agcaataaaa atgtgttcat
    10621 tagtcaatga gttgagaaaa caggaagata tgaaaacagt tgtttgtgta actggtcaac
    10681 acaaggagat ggttagccct gttttagatt tatttggagt tcaaccagac tatgatttag
    10741 aaattatgaa ggctaatcaa aacttgttct ctataacaat aagtatttta gaaaaaataa
    10801 aacctgtgtt agaaaaagaa caaccagata ttgttttggt tcacggtgat actactacga
    10861 catatgcagc agctttagcg gcattttatc taggaattaa agttggtcat gttgaagctg
    10921 gattacgaac ttacaacctg caaagtcctt tcccagaaga gttcaacaga caatcgactt
    10981 ctattattgc aaattatcat tttgctccta cagaattagc taaagaaaat ctaataaaag
    11041 agggtagaaa taatatctat gttactggaa atacagtgat tgatgcactt acaactacag
    11101 tacaaaagga ttatacacac cccgatctag atttaaacga tggaaatcgc ctcatcttat
    11161 tgactgctca tagacgcgaa aatctcggag aacctatgag acatatgttt agagctgtta
    11221 aacgagtttt aaatgaatat gacgatgtta aagtaattta tccaattcat aaaaatccat
    11281 tggtacggga aacagctaca gaaatttttg gagatacaga acgtattcag atcattgaac
    11341 ctttagatgt tcttgatttt cacaacttta tgaatcatag ttatatgatt ctaactgact
    11401 caggaggggt tcaagaagag gcaccttcgt taggaaagcc tgtattggtc atgcgagata
    11461 cgacagaaag acctgaagga gtagctgctg gaacgttgaa attggttggg actgatgagg
    11521 agactattta tcaaaacttt aagatgcttt tagacgatcc cgaagaatat aaaaaaatga
    11581 gtcaagctag taatccttat ggaaatggtg atgctagtaa acagattgtt cgaattttac
    11641 gtggaatttg agtgtgttca gataaagtaa tatagaaagg taccctacta tgaaaggtat
    11701 tattctagca ggtggttcgg ggacacgttt atatcctttg actcgcgctg catcaaaaca
    11761 acttatgccg gtttatgata aaccgatgat ttactaccca ctttcaacat tgatgttggc
    11821 tgggattagg gatattttga ttatttccac tccacaggat ttacatcgat tccaagagct
    11881 tcttcaagac ggatctgagt ttgggctcaa actttcttat gcagagcaac caagtccaga
    11941 tggtttggca caagccttta tcattgggga agagtttatt tctgatgata gcgttgcgct
    12001 gatcttaggt gacaatatct atcacggttc tgggctttcc aagatgctac aaaaggcagc
    12061 gagtaaggag tcgggagcaa ctgtttttgg ctaccatgtc aaggatccag agcgctttgg
    12121 tgtggttgag tttgatcagg atatgaaggc tatttctatt gaagaaaagc cagagcaacc
    12181 tcgttcaaac tatgcagtta caggtctcta tttctatgat aatgatgtag tagagattgc
    12241 caagagtatt aaaccaagtc ctcgtggtga actggaaatt acagatgtaa acaaggctta
    12301 cctagatcgt ggtgatttgt ctgttgagct tatgggacgt ggctttgctt ggctggatac
    12361 tggaactcat gaaagtttac tagaggcttc acagtacatc gaaacagtgc aacggatgca
    12421 aaatgttcag gtagcaaact tagaagaaat tgcttaccgt atgggctata tcagtcgaga
    12481 agatgtattg gccttagccc aaccacttaa gaaaaatgaa tacggacagt atctgctccg
    12541 tttgattgga gaagcataga tgacagataa ttttttcggt aagacgcttg cggcacgcaa
    12601 ggttgaagct attccaggca tgttggagtt tgatatcccc gttcatggag ataaccgtgg
    12661 ctggtttaaa gaaaatttcc aaaaggaaaa aatgcttcca cttggatttc cagagtcttt
    12721 ctttgcagaa ggaaaattgc aaaacaatgt atccttctca cgtaaaaatg tccttcgagg
    12781 tctccacgca gagccttggg ataagtacat ctctgtagca gatggaggga aagttctggg
    12841 ttcttgggtt gatctacgcg agggtgaaac ctttgggaat acctatcaga cagtgattga
    12901 tgcgagtaag ggaatctttg ttcctcgagg cgtagctaat ggctttcaag ttttatcaga
    12961 tacagtgtca tatagctatc tggtcaatga ttactgggct cttgaactca aacccaagta
    13021 tgcctttgtg aactacgctg atccaagcct tggtattgaa tgggaaaata ttgcagaagc
    13081 agaggtttca gaagcagaca aacatcatcc actacttaag gatgtaaaac ctttgaaaaa
    13141 agaagatttg gaataaggaa agaatatgac tgaatacaaa aatattatcg tgacaggtgg
    13201 agctggcttt atcggttcta actttgtcca ttatgtttac gagaactttc cagatgttca
    13261 tgtgacagtc ctagataagt tgacttatgc tggaaaccgc gcgaatattg aggaaatttt
    13321 aggtaatcgt gttgagttag ttgttggtga cattgctgat gcggagttgg tagacaagtt
    13381 ggctgctcaa gcagatgcta tcgttcatta tgcagcggaa agccacaatg ataattcgct
    13441 caatgatcca tcgccattta ttcatactaa cttcatcgga acctatactc ttttagaagc
    13501 tgctcgtaag tatgatattc gcttccacca tgtatcgaca gatgaagttt atggggatct
    13561 ccctttacgc gaagatttgc caggtcatgg tgaaggacca ggtgagaaat ttactgctga
    13621 aacaaaatac aacccaagct ctccgtactc atcaaccaag gcagcctcag atttgattgt
    13681 caaagcctgg gtgcgttctt ttggagtcaa ggcaacgatt tccaactgtt caaataacta
    13741 cggtccttat caacatatcg aaaaattcat cccacgtcag attactaaca tcctaagtgg
    13801 tatcaagcca aaactttacg gtgaaggtaa gaacgttcgt gattggattc ataccaatga
    13861 ccattcttca ggagtttgga caatcttgac aaaagggcaa atcggtgaaa cctacttgat
    13921 tggggctgat ggtgagaaga acaataagga agttttggaa cttatcctta aggaaatggg
    13981 acaagctgcg gatgcctatg atcatgtgac tgaccgtgca ggacatgacc ttcgctatgc
    14041 gattgatgct agcaagctcc gtgatgagtt ggggtggaaa cctgaattta ccaactttga
    14101 agctgggctc aaggcaacaa tcaagtggta tacagataac caagaatggt ggaaagcaga
    14161 gaaagaagct gttgaagcca attatgctaa gactcaggag attattacag tataaaaagc
    14221 aggaaatagc tgctttttat tgctatattg ggaagagtta catattagaa aggtctagag
    14281 atgattttaa ttacaggggc aaatggccaa ttaggaacgg aacttcgcta tttattggat
    14341 gaacgtaatg aagaatacgt ggcagtagat gtggctgaga tggacattac cgatgcagaa
    14401 atggttgaga aagtttttga agaggtgaaa ccgactttag tctaccactg tgcagcctac
    14461 accgctgttg atgcagcaga ggatgaagga aaagagttgg acttcgccat caatgtgacg
    14521 gggacaaaaa atgtcgcaag agcatctgaa aagcatggtg caactctagt ttatatttct
    14581 acggactatg tctttgacgg taagaaacca gttggacaag agtgggaagt tgatgaccga
    14641 ccagatccac agacagaata tggacgcact aagcgtatgg gggaagagtt agttgagaag
    14701 catgtgtcta atttctatat tatccgtact gcctgggtat ttggaaatta tggcaaaaac
    14761 ttcgttttta ccatgcaaaa tcttgcgaaa actcataaga ctttaacagt tgtaaatgac
    14821 cagtacggtc gtccgacttg gactcgtacc ttggctgagt tcatgaccta cctagctgaa
    14881 aatcgtaagg aatttggtta ttatcatttg tcaaatgatg cgacagaaga cacaacatgg
    14941 tatgattttg cagttgaaat tttgaaagat acagatgtcg aagtcaagcc agtagattcc
    15001 agtcaatttc cagccaaagc taaacgtccg ctaaactcaa cgatgagcct ggccaaagcc
    15061 aaagctactg gatttgttat tccaacttgg caagatgcat tgcaagaatt ttacaaacaa
    15121 gaagtgagat aagtagtaga atgattttct agtctaataa aagaggcaga taatgaactc
    15181 caaaggagct taagatgtac gattatcttg ttgttggtgc tggtctcttt ggcgcatagc
    15241 tttggctcag tttctattat cgctcacacc atccatcaga agtttaatct gaaggtaccc
    15301 aattatcgcc aagaagaaga ttgggctagg atgggtttac caatcacacg taaggaaatc
    15361 tctaattggc atatcaaggc aagtcaatac tatttagagt ccctttataa ccttttacga
    15421 gaaaagttgt tagaacaacc tcttcttcat gcggatgaaa cctcttatcg ggtcttagaa
    15481 agtgatagtc agctgaccta ctattggacc tttttgtctg ggaaagctga gaatcaagta
    15541 atcacgcttt accaccatga tcagtgtcgg agtggttcgg tagtgcaaga attcctagga
    15601 gattattctg gctatgtgca ttgtgatatg ttgcggcagt aacttaggac tttagtcctc
    15661 tagttctgtc tatgcgatag cagtccaagg tttaggagca aggcgacgct aagcttggta
    15721 aactgcgaac cgctagaagc ttatcgtcaa ctggaagaag ctgaacttgt tggatgttgg
    15781 gcgcatgtga gaaggaaatt ttttgaagtg ccccccaagc aagcagataa atcatcctta
    15841 ggagctaaag gtttagctta ttgtgatcag ttattttcct tggaaagaga ctgggaggct
    15901 ttgccagctg atgaacgact acagaaacgt caagaacatc tccaacccct aatggaagac
    15961 ttctttgctt agtgccgtcg tcagtcagtt ttatcgggtt caaaactagg aagggcaatt
    16021 gaatacagcc tcaagtatga agaaaccttt aagaccattt taaaagacgg acatctggtc
    16081 ctttccaata atctagctga acgcgccatt aaatcattgg ttatgggacg gagtaaaaga
    16141 gtccagtgga ctcttttagc ctaagctaaa ttttaaaaag cgagggtggt tattttctca
    16201 aagttttgaa ggagctaaag caagagctat tattatgagc ttattggaaa cagctaaacg
    16261 tcatcaacta aatagtgaga aatatctatc ctatcttcta gaatgtcttc caaacgagga
    16321 aactctcgta aacaaagagg ttttagaggc ctatttacca tggactaaag ttgtacaaga
    16381 aaagtgcaaa taagaaatct ccagattagg aactatccgt gagttcacta atctggagat
    16441 ttttcaatag acttcgttat tgggcggtta cgatatgatc actgacttcg tcagtcttat
    16501 ctacaacctc aaaactgtgt tttgagcaac ctgcggctag cttcctagtt tgctctttga
    16561 ttttcattga gtattagaac agaaaaaata cttggagtat ttgtttgtgt gtttattttt
    16621 atataacaaa ctataaacaa aacaaaaata taaaaaaaga gacaaaaaaa gaacagaaag
    16681 caattgacaa cggtttcata tagtgttata cttatggcat aaagttaatg gaaagaaggg
    16741 aaaaacttat ggtattagat tatttctttg acaaaaacct tgtgttttgc ttagaagcgg
    16801 ataatcaaga acaactcttt gatcaggttg caactttatt ggaagaacga gaaattgtca
    16861 ctccaactta tcgtgaagcc ttgatcacgc gtgaaaagtc atttccaact ggtttagata
    16921 tggaatttct aggaaaggac ttgccaaatg tagcgattcc tcatacagat attgttcata
    16981 atctagctga gaaagtggtg gttgttcgat tagaaaaacc agtaactttt cacaatatga
    17041 tagctcctga taaggaagta gaagtatccc tactcttctt tattattaac aattcaagtt
    17101 caagtcaaac aaatattctg gctcagttga tggacttttt cacaggaaat ggacatcttg
    17161 aagacctatc aaaaatttcc gaaccagaaa aactttatgc ttacattgct gaagcaaccg
    17221 cttaatcttg tctattaaaa aataaaatcg gaggaaatcc aaatgattaa aattcttgct
    17281 gcctgcggtg caggtgttaa ctcaagtcac caaattaaaa gtgctctaga agaagaactt
    17341 tcaaaccgtg gttatgatgt tcactgtgat gcagtcatgg tgaaagatgt aaacgaagac
    17401 cttatgaaag gttacgatat ctttacacca atcgctgcaa cagaccttgg ttttgaacca
    17461 ggtatcccag ttatcgaagc tgggccaatc ttattccgta tcccagcaat gagcgctcca
    17521 gtatttgaca atattgaagc agctattaaa gaacacggat taagctaatt ctaatttgtt
    17581 aacattcata taacataaaa aagggaggaa ctgttatgga tgcaatcttt gacctaatcg
    17641 gaaaggtttt caatcccatt ttagaaatgg gtggacctgt catcatgtta atcattttga
    17701 cagtattggc tttacttttt ggagtgaaat tctccaaagc gcttgaaggt ggtatcaaac
    17761 ttgccatcgc tcttacaggt atcggtgcta tcatcggtat gctaaatggt gctttctcag
    17821 catcacttgc aaaattcgtt gaaaacactg gtatccaatt gagtattacc gacgttggtt
    17881 gggcaccact cgcaacaatc acttggggtt ctgcttggac actatacttc ttgctcatca
    17941 tgttgattgt caacatagtg atgctagcta tgaagaaaac agatacactt gacgttgata
    18001 tctttgatat ctggcacttg tctatcacag gtctcttgat taaatggtat gctgataaca
    18061 atggtgtgag tcaaggggtt tcactcttta ttgctacagc agctatcgtc cttgtcggtg
    18121 tgttgaaaat tatcaactct gacttgatga aacctacatt tgatgacctt cttaacgccc
    18181 caagttcatc accaatgaca tcaactcaca tgaactacat gatgaaccca gttatcatgg
    18241 ttttggataa gatttttgaa aaattcttcc caggccttga taaatatgac tttgatgctg
    18301 ctaaattgaa caagaaaatc ggtttctggg gatctaaatt cttcatcggt ttcatccttg
    18361 gtatcgttat cggtattatg ggaactccac atccaattgc aggtgttgca gatgcagata
    18421 aatggcgtct tgttatcaaa ggatggttgt ctcttggttt gactgccggt gtatctttgg
    18481 aactcttctc acttatcggt tcatggttca tcgcagccgt agaaccacta tcacaaggta
    18541 ttacaaacgt tgctactaaa cgtcttcaag gacgtaaatt caatatcggt cttgactggc
    18601 cattcatcgc tggtcgtgct gaaatctggg cttgtgccaa cgtacttgca ccaatcatgt
    18661 tgattgaagc agtgcttctt tcaaaagttg gaaatggtat cttgccactt gcaggtatca
    18721 tcgctatggg tgttactcca gctctcttgg ttgtaactcg tggtaaattg ctccgtatga
    18781 ttatcttcgg aacactcttg ttgccactct tccttctttc aggtacactt attgcaccat
    18841 ttgcaacaga acttgctaaa ggtgtaggtg ccttcccaga aggtgtgagc caaactcaat
    18901 tgattactca ctctactctt gaaggaccaa tcgaaaaact tcttggttgg acaattggta
    18961 acactacaac tggtgatatc aaagcaatcc ttggtgcagt agtcttcctt gtattctata
    19021 tcggtatctt tgcttggtac agaaaacaaa tgatcaaacg taacgaagag tacgcagcaa
    19081 aagcaaaata atgcgctcct ataaaatata aactcaatga aaatcaaaga gcaaactagg
    19141 aaactagcca caggttgctc aaaacacagt tttgaggttg tagataagac tgacgaagtc
    19201 agctcaaaac atggttttga ggttgtagat gaaactgacg aagtcagtaa tcatatctac
    19261 ggcaaggcga cactgacgaa gtttgaattg gattttcgca gagtgtaaat tgttgaaact
    19321 aggttgtcat accatgtata ccacttgggc tttggcagta gctaactgca ctaaatataa
    19381 tataaggagg gttttatggg gaaaggccat tggaatcgga aaagagttta tagcattcgt
    19441 aagtttgctg tgggagcttg ctcagtaatg attgggactt gtgcagttct attaggagga
    19501 aatatagctg gagaatctgt agtttatgcg gatgaaacac ttattactca tactgctgag
    19561 aaacctaaag aggaaaaaat gatagtagaa gaaaaggctg ataaagcttt ggaaactaaa
    19621 aatgtagttg aaaggacaga acaaagtgaa cctagttcaa ctgaggctat tgcatctgag
    19681 aagaaagaag atgaagccgt aactccaaaa gaggaaaaag tgtctgctaa accggaagaa
    19741 aaagctccaa ggatagaatc acaagcttca agtcaagaaa aaccgctcaa ggaagatgct
    19801 aaagctgtaa caaatgaaga agtgaatcaa atgattgaaa acaggaaagt ggattttaat
    19861 caaaattggt actttaaact caatgcaaat tctaaggaag ccattaaacc tgatgcagac
    19921 gtatctacgt ggaaaaaatt agatttaccg tatgactgga gtatctttaa cgatttcgat
    19981 catgaatctc ctgcacaaaa tgaaggtgga cagctcaacg gtggggaagc ttggtatcgc
    20041 aagactttca aactagatga aaaagacctc aagaaaaatg ttcgccttac ttttgatggc
    20101 gtctacatgg attctcaagt ttatgtcaat ggtcagttag tggggcatta tccaaatggt
    20161 tataaccagt tctcatacga tatcaccaaa tacctttaca aagatggtcg tgagaatgtg
    20221 attgctgtcc atgcagtcaa caaacagcca agtagccgtt ggtattcagg aagtggtatc
    20281 tatcgtgatg tgactttaca agtgacagat aaggtgcatg ttgagaaaaa tgggacaact
    20341 attttaacac caaaacttga agaacaacaa catggcaagg ttgaaactca tgtgaccagc
    20401 aaaatcgtca atacggacga caaagaccat gaacttgtag ccgaatatca aatcgttgaa
    20461 cgaggtggtc atgctgtaac aggcttagtt cgtacagcga gtcgtacctt aaaagcacat
    20521 gaatcaacaa gcctagatgc gattttagaa gttgaaagac caaaactctg gaccgtttta
    20581 aatgacaaac ctgccttgta cgaattgatt acgcgtgttt accgtgacgg tcaattggtt
    20641 gatgctaaga aggatttgtt tggttaccgt tactatcact ggactccaaa tgaaggtttc
    20701 tctttgaatg gtgaacgtat taaattccat ggagtatcct tgcaccacga ccatggggcg
    20761 cttggagcag aagaaaacta taaagcagaa tatcgccgtc tcaaacaaat gaaggagatg
    20821 ggagttaact ccatccgtac aacccacaac cctgctagtg agcaaacctt gcaaatcgca
    20881 gcagaactag gtttactcgt tcaggaagag gcctttgata cttggtatgg tggcaagaaa
    20941 ccttatgact atggacgttt ctttgaaaaa gatgccactc acccagaagc tcgaaaaggt
    21001 gaaaaatggt ctgattttga cctacgtacc atggtcgaaa gaggcaaaaa caaccctgct
    21061 atcttcatgt ggtcaattgg taatgaaata ggtgaagcta atggtgatgc ccactcttta
    21121 gcaactgtta aacgtttggt caaggttatc aaggatgttg ataagactcg ctatgttacc
    21181 atgggagcag ataaattccg tttcggtaat ggtagcggag ggcatgagaa aattgctgat
    21241 gaactcgatg ctgttggatt taactattct gaagataatt acaaagccct tagagctaag
    21301 catccaaaat ggttgattta cggttcagaa acatcatcag caacccgtac acgaggaagt
    21361 tactatcgcc ctgaacgtga attgaaacat agcaatggac ctgagcgtaa ttatgaacag
    21421 tcagattatg gaaatgatcg tgtgggttgg gggaaaacag caaccgcttc atggactttt
    21481 gaccgtgaca acgctggcta tgctggacag tttatctgga caggtacgga ctatattggt
    21541 gaacctacac catggcacaa ccaaaatcaa actcccgtta agagctctta ctttggtatc
    21601 gtagatacag ccggcattcc aaaacatgac ttctatctct accaaagcca atgggtttct
    21661 gttaagaaga aaccgatggt acaccttctt cctcactgga actgggaaaa caaagaatta
    21721 gcatccaaag tagctgactc agaaggtaag attccagttc gtgcttattc gaatgcttct
    21781 agtgtagaat tgttcttgaa tggaaaatct cttggtctta agactttcaa taaaaaacaa
    21841 accagcgatg ggcggactta ccaagaaggt gcaaatgcta atgaacttta tcttgaatgg
    21901 aaagttgcct atcaaccagg taccttggaa gcaattgctc gtgatgaatc tggcaaggaa
    21961 attgctcgag ataagattac gactgctggt aagccagcgg cagttcgtct tattaaggaa
    22021 gatcatgcga ttgcagcaga tggaaaagac ttgacttaca tctactatga aattgttgac
    22081 agccagggga atgtggttcc aactgctaat aatctggttc gcttccaatt gcatggtcaa
    22141 ggtcaactgg tcggtgtaga taacggagaa caagccagcc gtgaacgcta taaggcgcaa
    22201 gcagatggtt cttggattcg taaagcattt aatggtaaag gtgttgccat tgtcaaatca
    22261 actgaacaag cagggaaatt cacccttact gcccactctg atctcttgaa atcgaaccaa
    22321 gtcactgtct ttactggtaa gaaagaagga caagagaaga ctgttttggg gacagaagtg
    22381 ccaaaagtac agaccattat tggagaggca cctgaaatgc ctaccactgt tccgtttgta
    22441 tacagtgatg gtagccgtgc agaacgtcct gtaacctggt ctcaagtcga tgttagtcaa
    22501 gcaggagttg tgactgttaa aggtacagca aatggacgtg aggtagaggc tcatgtcaag
    22561 gttctagcaa ttgcgaaaga attaccaaca gttaaacgta ttgctccaaa tactgacttg
    22621 aattctgtag acaaatctgt ttcttatgtt ttgactgatg gaagtgtaca agagtacgaa
    22681 gtagatagct gggagattac ggaagtagat aaagccaaac tttcagtagc tggatcacgt
    22741 attcaaatga ctggtcagtt agctggagaa actattcatg caacccttgt ggtagaagaa
    22801 ggaaatgctg cagcacctgt agtgccaact gttactgttg gaggtgaagc agtaacaggt
    22861 cttactagtc gacaaccaat gcaatatcgt actctatctt atggtgccca attgccagaa
    22921 gtcacagcaa gtgctgaaaa tgctgatgtg acagttcttc aagcaagcgc agcaaacggc
    22981 atgcgtgcga gcatctttat tcagcctaaa gatggtggcc ctcttcaaac ctatgcaatt
    23041 caattccttg aagaagcgcc aaaaattgct cacttgagct tgcaagtgga aaaagctgac
    23101 agtctcaaag aagaccaaac tgtcaaattg tcggttcgag ctcactatca agatggaacg
    23161 caagctgtat taccagctga taaagtaacc ttctctacaa gtggtgaagg ggaagtcgca
    23221 attcgtaaag gaatgcttga gttgcataag ccaggagcag tcactctgaa cgctgaatat
    23281 gagggagcta aagaccaagt tgaactcact atccaagcca atactgagaa gaagattgcg
    23341 caatccatcc gtcctgtaaa tgtagtgaca gatttgcatc aggaaccaag tcttccagca
    23401 acagtaacag ttgagtatga caaaggtttc cctaaaactc ataaagtcac ttggcaagct
    23461 attccgaaag aaaaactaga ctcctatcaa acatttgaag tactaggtaa agttgaagga
    23521 attgaccttg aagcgcgtgc aaaagtctct gtagaaggta tcgtttcagt tgaagaagtc
    23581 agtgtgacaa ctccaatcgc agaagcacca caattaccag aaagtgttcg gacatatgat
    23641 tcaaatggtc acgtttcatc agctaaggtt gcatgggatg cgattcgtcc agagcaatac
    23701 gctaaggaag gtgtctttac agttaatggt cgcttagaag gtacgcaatt aacaactaaa
    23761 cttcatgttc gcgtatctgc tcaaactgag caaggtgcaa acatttctga ccaatggacc
    23821 ggttcagaat tgccacttgc ctttgcttca gactcaaatc caagcgaccc agtttcaaat
    23881 gttaatgaca agctcatttc ctacaataac caaccagcca atcgttggac aaactggaat
    23941 cgtactaatc cagaagcttc agtcggtgtt ctgtttggag attcaggtat cttgagcaaa
    24001 cgctccgttg ataatctaag tgtcggattc catgaagacc atggagttgg tgtaccgaag
    24061 tcttatgtga ttgagtatta tgttggtaag actgtcccaa cagctcctaa aaaccctagt
    24121 tttgttggta atgaggacca tgtctttaat gattctgcca actggaaacc agttactaat
    24181 ctaaaagccc ctgctcaact caaggctgga gaaatgaacc actttagctt tgataaagtt
    24241 gaaacctatg ctgttcgtat tcgcatggtt aaagcagata acaagcgtgg aacgtctatc
    24301 acagaggtac aaatctttgc gaaacaagtt gcggcagcca agcaaggaca aacaagaatc
    24361 caagttgacg gcaaagactt agcaaacttc aaccctgatt tgacagacta ctaccttgag
    24421 tctgtagatg gaaaagttcc ggcagtcaca gcaagtgtta gcaacaatgg tctcgctacc
    24481 gtcgttccaa gcgttcgtga aggtgagcca gttcgtgtca tcgcgaaagc tgaaaatggc
    24541 gacatcttag gagaataccg tctgcacttc actaaggata agagcttact ttctcataaa
    24601 ccagttgctg cggttaaaca agctcgcttg ctacaagtag gtcaagcact tgaattgccg
    24661 actaaggttc cagtttactt cacaggtaaa gacggctacg aaacaaaaga cctgacagtt
    24721 gaatgggaag aagttccagc ggaaaatctg acaaaagcag gtcaatttac tgttcgaggc
    24781 cgtgtccttg gtagtaacct tgttgctgag gtcactgtac gagtgacaga caaacttggt
    24841 gagactcttt cagataaccc taactatgat gaaaacagta accaggcctt tgcttcagca
    24901 accaatgata ttgacaaaaa ctctcatgac cgcgttgact atctcaatga cggagatcat
    24961 tcagaaaatc gtcgttggac aaactggtca ccaacaccat cttctaatcc agaagtatca
    25021 gcgggtgtga ttttccgtga aaatggtaag attgtagaac ggactgttgc acaaggaaaa
    25081 gttcagttct ttgcagatag tggtacggat gcaccatcta aactcgtttt agaacgctat
    25141 gtcggtccag agtttgaagt gccaacctac tattcaaact accaagccta cgacgcagac
    25201 catccattca acaatccaga aaattgggaa gctgttcctt atcgtgcgga taaagacatt
    25261 gcagctggtg atgaaatcaa cgtaacattt aaagctatca aagccaaagc tatgagatgg
    25321 cgtatggagc gtaaagcaga taagagcggt gttgcgatga ttgagatgac cttccttgca
    25381 ccaagtgaat tgcctcaaga aagcactcaa tcaaagattc ttgtagatgg aaaagaactt
    25441 gctgatttcg ctgaaaatcg tcaagactat caaattacct ataaaggtca acggccaaaa
    25501 gtctcagttg aagaaaacaa tcaagtagct tcaactgtgg tagatagtgg agaagatagc
    25561 tttccagtac ttgttcgcct cgtttcagaa agtggaaaac aagtcaagga ataccgtatc
    25621 cacttgacta aggaaaaacc agtttctgag aagacagttg ctgctgtaca agaagatctt
    25681 ccaaaaatcg aatttgttga aaaagatttg gcatacaaga cagttgagaa aaaagattca
    25741 acactgtatc taggtgaaac tcgtgtagaa caagaaggaa aagttggaaa agaacgtatc
    25801 tttacagcga ttaatcctga tggaagtaag gaagaaaaac tccgtgaagt ggtagaagtt
    25861 ccgacagacc gcatcgtctt ggttggaacc aaaccagtag ctcaagaagc taaaaaacca
    25921 caagtgtcag aaaaagcaga tacaaaacca attgattcaa gtgaagctag tcaaactaat
    25981 aaagcccagt taccaagtac aggtagtgcg gcaagccaag cagcagtagc agcaggttta
    26041 actcttctag gtttgagtgc aggattagta gttactaaag gtaaaaaaga agactagaaa
    26101 ttttgatacc ttctttatca ttaaagacca aagaaaaact cttgtcctaa atagaagata
    26161 aagagcctga gacaaaatag ttctcaacca caaaaaagct agatatttcc aattgtggaa
    26221 ctctagcttt ttaattttga gtcattctct tattgtatag tagattgaaa ctagaatagt
    26281 acacttttac ttctaaaaca ttgttagaaa tcgatttgat tgtccttatc gatttgtcct
    26341 gttcttattt cattttacta tattttagga ggttattggc cataagtacc aatcccatgt
    26401 caattctcac ttgacgcttc cctctcagat tacatctctt gtaacccaaa gaagccttta
    26461 tctgcccaaa gacaggttcc acatcaatct tgcgttgagc gaaaatctgt ctaccttggg
    26521 gagataaaag cgcctgacat tctttagctt tcaagttttg atagcgttcg ttcatataca
    26581 gtcccttttg aggggctgat tcaggttcgt cggcgtagta aaccttgatt tcttgttgaa
    26641 agtctgtctg tgttttctga tatttggtat ggtgaaaacg ataataccag ccatcaggat
    26701 gtgtgtagct atcctccttg tcattatagt gccaattcgc taagttttta gctgactctt
    26761 tataccctct cttttgtttc ttatcaaaca tggcatattt aatcagaagg ggagtgattt
    26821 cttcttgaaa atagacctgt atctgttctt gaagtttggc ggagaatttc tctgtcgttt
    26881 tcttccacac aaaactatac ttgttggcat tggcttcaat cttagttctg tcaataaaca
    26941 aacaatctaa ggtcactaac tcttccattt ttcaattttt cgaccagaga aaatcccttg
    27001 tgaataggca aatagaagag tagatacaag cattttaggg tgataagacg ggcgatcaaa
    27061 ggcatgatag gaggtgtaga agtgacgttc ctccaaggta ttcaccactt tttcaatagt
    27121 aaaaacgaga tgatcttgtg gtaagaagga actgatttct agtggtaaag ttgtttgatt
    27181 tgtgttatag tgaatatgca tgggatagcc tttctaaatg gtttattggg caattctatt
    27241 ttaccaagac tatcgtaacc atgcaaaaaa gcaacggcaa atccgttgcc tttttggata
    27301 taagagacta ttgtcccaga ctcttttctt tgtataaaat tgtatagtgt tattgtctat
    27361 actattttgt agagaaagca aaaatcccta gaaaatgctt agggattcga ctatttcatt
    27421 aagaacaatt caacaatcat ttctatatac atgataaggg ttaataagaa tcctgcacgc
    27481 cagaagaatt tgataaattt aggatagtaa aagctgcgtt ttttcaaaag gaagaaaaag
    27541 acgagaataa tggctaggat cgagagggct agccccagtc tagggaggaa attatgggta
    27601 aaggttttag ctgtaatcaa gtaatactca aataccaaga ctggaaaagc caaatccgca
    27661 aagtttcgtc ctagtttttt taatctaaaa agtttggtta ggataatgca gacgactaag
    27721 gtcagtatca ataataaaat agatgctaat ttcattaaaa tcatacccat attgtatcat
    27781 aaaaaaaggc taatggaaac gagaaacaga ggaatttata ggaaagtcag gcttttgaga
    27841 ttgtgggtga tttttgttat aatgaaagtc atgaaatctt ataatacctt gaatgattat
    27901 tatcgaaaac tctttggaga aaagactttt aaagtcccta ttgatgcggg atttgactgt
    27961 cccaatcgtg atggaactgt ggctcatgga ggctgtactt tttgtacggt ttcaggttct
    28021 ggagatgcta ttgtagcccc agatgcgcct atccgtgagc aattttataa ggaaattgac
    28081 tttatgcacc gtaagtggcc ggatgttcag aagtatctgg tttattttca aaattttacc
    28141 aacacccatg aaaaggtgga agttgttcga gagcgttatg aacaggctat caatgagcca
    28201 ggtgtggtag gtatcaatat tggaacgcgc ccagactgtt taccagacga aaccatcgaa
    28261 tatttggctg agttatcgga gtgcatgcat gtgacggttg aattgggctt gcaaactact
    28321 tatgaagcaa cctctgacct gattaaccgt gcgcattctt atgaattgta ggtggaaacg
    28381 gtcaagcgtt tgagaaagta tccaaaaatt gagattgttt cccatttgat caatggtttg
    28441 cctggtgaaa cccatgagat gatggttgaa aatgtccgcc gttgtgtcac gaataacgat
    28501 attcaaggga ttaaactgca tttgctccat cttatgacaa atactcgtat gcaacgagat
    28561 taccatgagg gacgtttaca attgatgagt caggacgaat atgtcagggt catctgtgac
    28621 caactggaaa tcattcccaa acatatcgtc atccatcgaa ttacaggaga tgcgcctagg
    28681 gatatgctgc ttgggcttat gtggagcctc aataaatggg aagctctcaa cagcattgag
    28741 atggagatgc gacgtcgtgg aagtgttcaa ggatgcaagg ctgtaaaaca ggagtttgaa
    28801 aatgaaaaga ccacttgaga tggcacatga ttttttggct gaggtcgtga caaaagagga
    28861 tgtcgtagtg gatgcgacta tgggaaatgg tcatgacacg ctttttttag ccaagctagc
    28921 caagcaagtc tatgcctttg atattcagaa gcaagccttg gaaaagaccc aagagcgttt
    28981 gcatcaggct ggcttgacaa atgcccagtt aatcttgcaa ggccatgaga cactggacca
    29041 gtttgtgaca aaagctaagg cagggatttt taatttgggc tatttgccgt cagctgataa
    29101 gtctgtcatc acccgaccgc agacaacgat tgaggcatta gaaaagctat gtggcttact
    29161 tgtcaaaggt ggacgaattg ctattatgat ttactatggt catgaaggag gcgacctcga
    29221 gagagatgct gtcttggatt ttgtgatcca gttgaaccaa caagagtaca cagctgccat
    29281 ttaccgaact ttaaaccaag tcaacaaccc gccgttttta gtgatgattg aaaaattaga
    29341 gagatacaga catggataaa caatacctac acgaaaagct ggatgccatg cgccagaatt
    29401 ttgttgaatc aacccaccac gaacgagcgg tgggcgtgct agaccaagcg catatgagca
    29461 aaaaaatgct taaaatcaag aaaaaattag ttgctcttga aatggaacgg tgccagagaa
    29521 aaattgagca caaagactgt tccaagattg accaaaaaat caaagagcag aaggagatat
    29581 ttgaatcctg ttgtaaaaaa gattaaggag gacgtgcgtg gaattactga tttacctcat
    29641 cctattttta ctggttttga ttgtctcaag tacaaccaat aagctcctgc cctttttgcc
    29701 tctccctttg gtgcagattc ttttgggaat tgtgattggt ctctttttac ccaatactga
    29761 ctttcacctt aatacggagt tgtttttggc actggttatc ggacccttgc ttttccgaga
    29821 ggctgaagaa gcagatgtta cggctatttt aaaacactgg cgaatcattg tttatctcat
    29881 atttccagtg atttttatct cgaccctgag tttgggtggc ttggcccatc ttctttggtt
    29941 cagccttccc ttggcagctt gcttggctgt tggtgcagcc cttggtccta cggacttggt
    30001 ggcctttgcc tctctttcgg agcgttttag ctttcctaag cgcgtgtcca atattcttaa
    30061 gggcgaagga ctcttgaatg atgcttctgg tttggtggct tttcaggtag ctttgacagc
    30121 ttggacaact ggagcttttt ctctggggca agctagcagt tcgctcatct tttcaatcct
    30181 aggcggtttt ttaattggat ttttaacagc catgaccaac cgcttcctcc ataccttctt
    30241 gctaagtgtg cgcgcaacgg atattgccag tgaactttta ttagaattga gtttgcctct
    30301 agtgaccttc tttctggcag aagaagtcca tgtttcaggg attattgccg tcgtagttgc
    30361 tggaatttta aaggcaagtc gcttcaagaa aatcacgctc ctcgaagccc aagtggatac
    30421 ggtgaccgag acggtctggc atacagtgac ctttatgctc aacggttctg tctttgtgat
    30481 tttagggatg gagttggaaa tgatagcaga acctatcttg accaatccaa tctataatcc
    30541 tctactttta ttgctatctc tcatcgccct tacctttgtc ctctttgtca ttcgttttat
    30601 tatgatctat ggctattatg cctatagaac ccgacgcctc aagaaaaagc taaataagta
    30661 tatgaaggac atgtttctct tgaccttttc aggtgttaag ggaacggtgt cgattgctac
    30721 gattctcttg ataccaagta atctagaaca ggagtatcct ctcttgcttt tccttgttgc
    30781 aggtgtgacg cttgtcagct ttttaacagg tctcttggtc ttgcctcatc tttctgatga
    30841 agaggaagaa agcaaggatt atctcatgca tatcgccatt ttgaatgaag taacgctaga
    30901 gttggaaaaa gagttggaag acaccagaaa taaacttccc ctctatgcgg ctattgacaa
    30961 ttatcatgga cgtattgaaa atctcatttt aagccaagaa aaccaggatg atcaagaaga
    31021 ttgggctgct ttgaaactct tgattcttag tattgaaagt gatggtttgg agcaggccta
    31081 tgaagagggg aacattagca atcgtgctta ccgagtttac caacgttatc tgaaaaatat
    31141 agaacaagga atcaatcgta aacttgcctc aagactgacc tattatttcc ttgtttcttt
    31201 gcggatttta cgttttctcc ttcatgaagt ttttactctc ggaaagacct tccgtagctg
    31261 gaagaacaag gagcaaagcc gtctccgtgc tcttgattat gaccaaattg cagagctcta
    31321 tcttgccaat acagagatga ttattgaaag tttggaaaac ctgaagggag tctacagacg
    31381 ctctttgatt agttttatgc aggagtctcg tcttcgagaa acagctatta tcagcagtgg
    31441 tgcctttgtc gaacgggtta tcaatcgtgt caaacccaac aatatcgatg aaatgctgag
    31501 aggctattat ctggagcgca agttgatttt cgaatacgaa gaaaaacgat tgattacgac
    31561 taagtatgcc aagaaattac gacaaaatgt aaataactta gagaactatt ccttgaagga
    31621 agctgccaat accctaccgt atgatatggt ggaattggta agaagaaatt agttaatact
    31681 cttcgaaaat ctcttcaaac cacgtcagcg tcgccttacc gtatgtatgt gactgacttc
    31741 gtcagtctta tctacaacct caaaacagtg ttttgagcag cttgcggcta gctttctagt
    31801 ttgctctttg attttcattg agtataagat tataaatgaa ggagtatgac atgaaaaagt
    31861 ggtggaaaga gctgatagac aaacctttat taaaagcttt tttgcattat tatcaagcat
    31921 cagatagtga gttgaccagt gtcgcagtag cctactattg gttgatttcg attttccccc
    31981 tgcttttggt ggtggtcaat atcctcccct attttcagat tcctgtggga gaatttttgg
    32041 gctttatgaa agacgtcctg cccccaagcc tctatgaagg tgtggaaaaa atagccagag
    32101 aagtcttgac gcaaccttca acaggtttat tgagtttttc tgttttatcg gcgttatgga
    32161 gtttttcaaa atctatgaat ttcttgcaaa aagcttttaa caaggcttat ggcgtcgaaa
    32221 agagtcgtgg acttatttcc catcagatgc taagtctcct agtcagtttt ggcttgcagc
    32281 tcctctttgc gtttgccttg tttttgatct tatttggtca gatgattttg gctttacttg
    32341 ctcattattg gacaaaagat ggcattatct atcaggcttt acaagggttg gcaggtcctc
    32401 tgatttacgc cctcctcttt gctatcttgg tcatgcttta ttattttctt cccaatctat
    32461 ctaacaggaa aattagctat accctaccag gcagtgcctt tgttctctta gtgattttag
    32521 gtttactaac tctgttctct agttatctca attactatgt ccatcatttg gtggatgtcc
    32581 gaattttagg ttccgttctt cttgttgtta tgatgttttg gtttatcttg attgctaaaa
    32641 ttgttatcct aggcgcggtg atcaatgcca gtatgcagag tttgaaagat ccggtcttta
    32701 aaaaagacta actatttatc cattacaaaa gcgcatagta tcaagattct ataaagcctt
    32761 gatactatgc gcttttgtgc tttgacagtt ttttgtatag tttttgtcca agatttctaa
    32821 atcatctgat taaattccta ttttccccta tcattgtccc tgttttttcg aaaatggaag
    32881 gagtataata aatctatcaa taaaatgata ggaggaactt tatgggtcat attttctttt
    32941 ttctaagtgt ctttttggca gggattctat ccttcttttc tccttgtatc ttacctttgt
    33001 taccggtcta tacaggagtg ttactagatg ataaggatgg tgctcaggct tctagcggca
    33061 aattttcaat ctcagttact agtttattac gaactctggc ctttatagca ggaatttcct
    33121 ttatatttat tttgttgggc tatggagctg gttttttagg cgatttgctt tatgcttctt
    33181 ggttccaata tcttactggg gcaattatta tccttcttgg tttgcaccaa atggagattc
    33241 tacactttaa ggggctttat aaggaaaaga ggctacaact gcaaggacag gggcaaaatg
    33301 gtaagggcta tagtcaggca tttttattgg gcttgacctt tagttttgct tggacgcctt
    33361 gcgtggggcc ggttctgggg tctgttttgg ccttggcggc ttcaggtggt tcaggagctt
    33421 ggcagggagc tggtctcatg ttggtgtata cgctgggctt ggcgctacca ttcttgcttc
    33481 tagctctgac ctctagttat gttttgaaac atttccgaaa acttcatccc tatctcggaa
    33541 tcctcaaaaa agtgggtggt tttctcatta ttgtgatggg attcttggtt ctgtttggaa
    33601 atgcttcaat tttaagtcaa ttatttgaat aaaatggaaa ggaatatcaa tatgaaaaaa
    33661 tggcaaacat gtgttcttgg agcaggttcg ctcctttgtt tgacggcttg ttcaggcaag
    33721 tccgtgacta gtgaacacca aacgaaagat gaaatgaaga cggagcagac agctagtaaa
    33781 acaagcgcag ctaaagggaa agaggtggct gattttgaat tgatgggagt agatggcaag
    33841 acctaccgtt tatctgatta caagggcaag aaagtctatc tcaaattctg ggcttcttgg
    33901 tgttctatct gtctggctag tcttccagat acggatgaaa ttgctaaaga agctggtgat
    33961 gactatgtgg tcttgacagt agtgtcacca ggacataagg gagagcaatc tgaagcggac
    34021 tttaagaatt ggtataaggg attggattat aaaaatctcc cagttctagt tgacccatca
    34081 ggcaaacttt tggaaactta tggtgtccgt tcttacccaa cccaagcctt tatagacaaa
    34141 gaaggcaagc tggtcaaaac acatccagga ttcatggaaa aagatgcaat tttgcaaact
    34201 ttgaaggaat tagcctagga ggcgtcttat gaatgataag ttaaaaatct tcttgttgct
    34261 aggagtattt tttctagcca taaccggttt ctatgttcta ttgatacgaa atgcagggca
    34321 gacagatgcc tcgcaaattg aaaaggcggc agttagccaa ggaggaaaag cagtgaaaaa
    34381 aacagaaatt agtaaagacg cagacttgca cgaaatttat ctagctggag gttgtttctg
    34441 gggagtggag gaatatttct cacgcgttcc cggggtgacg gatgccgttt caggctatgc
    34501 aaatggtaga ggagaaacaa ccaagtacga attgattaac caaacaggtc atgcagaaac
    34561 cgtccatgtc acctatgatg ccaagcaaat ttctctcaag gaaatcctgc ttcactattt
    34621 ccgcattatc aatccaacca gcaaaaataa acaaggaaat gatgtgggga cccagtaccg
    34681 tactggtgtt tattacacag atgacaagga tttggaagtg attaaccaag tctttgatga
    34741 ggtggctaag aaatacgatc aacctctagc agttgaaaag gaaaacttga agaattttgt
    34801 ggtggctgag gattaccatc aagactatct caagaaaaat ccaaatggct actgccatat
    34861 caatgttaat caggcggcct atcctgtcat tgatgccagc aaatatccaa aaccaagtga
    34921 tgaggaattg aaaaagaccc tgtcacctga ggagtatgca gttacccagg aaaatcaaac
    34981 agaacgagct ttctcaaacc gttactggga taaatttgaa tccggtatct atgtggatat
    35041 agcaactgga gaacctctct tttcatcaaa ggacaagttt gagtctggtt gtggctggcc
    35101 tagttttacc caacccatca gtccagatgt tgtcacctac aaggaagata agtcctacaa
    35161 tatgacgcgt atggaagtgc ggagccgagt aggagattct caccttggcc atgtctttac
    35221 ggatggtcca caggacaagg gcggcttacg ttactgtatc aatagcctct ctatccgctt
    35281 tattcccaaa gaccaaatgg aagaaaaagg ctacgcttat ttactagatt atgttgatta
    35341 atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtatg tagggttact
    35401 gacttcgtca gttttatcta cagcctcaaa gctatacttt gagctgactt cgtcagtttt
    35461 atctacagcc tcaaagctat actttgagct gacttcgtca gttttatcta caacctcaaa
    35521 gctatacttt gagctgactt cgtcagtttt atctacaacc tcaaagcggt actttgagaa
    35581 acctgtggct atcttcctag tttacttttt gatttttatt gagtataaga ggtctttcct
    35641 aagcagttag aggagaattt tgctatactg atactagtaa gtggtaaagg agcagatcat
    35701 gacctacaca atcttaatcg tagaagatga atatctggta agacaaggtt tgactaaact
    35761 ggtcaatgta gcagcctacg atatggaaat catcggtcag gctgaaaatg gaaggcaggc
    35821 ttgggaattg atccaaaagc aggtgccaga tatcatttta accgatatca acatgcctca
    35881 tctaaatggc atccagttgg ccagtctggt acgagaaacc tatcctcagg ttcatttggt
    35941 ctttttaaca ggttacgatg attttgatta tgccttgtct gctgtcaaac taggtgtgga
    36001 cgactacctg ctcaaaccct tttctcgtca ggatattgag gaaatgttgg ggaaaatcaa
    36061 acaaaaacta gacaaggaag agaaagaaga gcagttacaa gatttattga ctaataggtt
    36121 tgaaggaaac atggcccaga aaatccagtc tcatctggct gatagccaat ttagtttaaa
    36181 gtctttagcc agtgacttag gttttagtcc gacctatctg agttccttga ttaagaaaga
    36241 gttgggcttg ccttttcagg attatctggt gagagaacgt gttaaacaag ccaagctctt
    36301 gcttttaact acagatctga agatttatga gatcgcagag aaggttggtt ttgaagatat
    36361 gaactatttt acccaacgtt ttaagcagat tgcaggtgtg acacctcgtc agtttaagaa
    36421 gggagaagac cgatgaagcg ttcttctctt ttagttagaa tggttatttc catctttctg
    36481 gtctttctca ttctcctagc tctggttgga actttctact atcaatcaag ttcttcagcc
    36541 attgaggcca ccattgaggg caacagccaa acgaccatca gccagactag ccactttatt
    36601 cagtcttata tcaaaaaact agaaaccacc tcgactggtt tgacccagca gacggatgtt
    36661 ctggcctatg ctgagaatcc cagtcaagac aaggtcgagg gaatccgaga tttgtttttg
    36721 accatcttga agtcagataa ggacttgaaa actgttgtgc tggtgaccaa atctggtcag
    36781 gtcatttcta cagatgacag tgtgcagatg aaaacttcct ctgatatgat ggctgaggat
    36841 tggtaccaaa aggctattca tcagggagct atgcctgttt tgactccagc tcgtaaatca
    36901 gatagtcagt gggtcatttc tgtcactcaa gaacttgttg atgcaaaggg agccaatctt
    36961 ggtgtgcttc gtttggatat ttcttatgaa actctggaag cctatctcaa tcaactccag
    37021 ttggggcagc agggctttgc cttcattatc aatgaaaacc atgaatttgt ctaccatcct
    37081 caacacacag tttatagttc gtctagcaaa atggaggcta tgaaacccta catcgagaca
    37141 ggtcagggtt atactcctgg tcacaaatcc tacgtcagtc aagagaagat tgcaggaact
    37201 gattggacgg tacttggcgt gtcatcattg gaaaagttag accaggttcg gagtcagctc
    37261 ttgtggacct tgcttggggc cagtgtcaca tctcttcttg tctgtctctg cttagtgtgg
    37321 ttcagtctta aacgctggat tgctcctttg aaggatttga gagaaaccat gttggaaatt
    37381 gcttctggtg ctcaaaatct tcgtgccaag gaagttggtg cctatgaact gagagaagta
    37441 actcgccaat ttaatgccat gttggatcag attgatcagt tgatggtagc tattcgtagc
    37501 caggaagaaa cgacccgtca gtaccaactt caagcccttt cgagccagat taatccacat
    37561 ttcctctata acactttgga caccatcatc tggatggctg aatttcatga tagtcagcga
    37621 gtggtgcagg tgaccaagtc cttggcaacc tatttccgct tggcgctcaa tcaaggcaag
    37681 gacttgattt gtctctctga cgaaatcaat catgtccgcc agtatctctt tatccagaaa
    37741 caacgctatg gagataagct ggaatacgaa attaatgaaa atgttgcctt tgataattta
    37801 gtcttaccca agctggtcct acaacccctt gtagaaaatg ctctttacca tggcattaag
    37861 gaaaaggaag gtcagggcca tattaaactt tctgtccaga aacaggattc gggattggtc
    37921 atccgtattg aggatgatgg cgttggcttc caagatgctg gtgatagtag tcaaagtcaa
    37981 ctcaaacgtg ggggagttgg tcttcaaaat gtcgatcaac ggctcaaact tcattttgga
    38041 gccaattacc agatgaagat tgattctaga ccccaaaaag ggacgaaagt tgaaatatat
    38101 ataaatagaa tagaaactag ctaactccca gtcaattctg ggagttttat gcgtaattgg
    38161 gcaaacttta taggttgtct atcttgctaa aaacaagaaa aaacggtatg atagaagagt
    38221 gacaacagag gtaacagaga tgaacgagac acagattcaa agagaaacac gtcaggtagt
    38281 ggaagatgtt ttagaaaaga ccaatttgaa gcagggggtc ctttttgttt taggtctatc
    38341 ttctagtgag gtgctaggtg gtcagattgg gaaagaatcc agtcaagaga ttggtgagct
    38401 gatagtggag actatcttgg gtattctagg tagtagaggt atccatttag cagttcaagg
    38461 ttgtgagcat gtcaatcggg ctctcgttgt tgaacgtcag gtggcagagc agtttgatct
    38521 ggaaattgtc agtgtccatc ctactcttca tgcaggaggt tcggggcagt tggcagcctt
    38581 taagtttatg caggatccag ttgaggttga atttatcaag gctcatgctg gattggatat
    38641 cggagacact gcaattggca tgcatgtcaa gcatgttcag gttccgattc gccctatttt
    38701 gagagagatt ggtcatgccc atgtaacggc actggctagt cgtccaaaat taatcggagg
    38761 tgcgcgtgcg cactatccgc aagacgctat tagaaagtct tgagaatgaa taaaggagat
    38821 aaaagtgttt aaaaaagacc gtttttcaat tcgtaagatt aagggagttg taggctctgt
    38881 atttcttgga agccttttga tggctccttc tgtagtggac gcagccacct atcactatgt
    38941 aaataaagag attatttcac aagaagctaa agatttaatt cagacaggaa agcctgacag
    39001 gaatgaagtt gtatatggtt tggtgtatca aaaagatcag ttgcctcaaa cagggacaga
    39061 agcatctgtt ttgacagctt ttggtttgct gactgttggg agcttgcttt taatctacaa
    39121 gagaaagaaa attgctagcg tctttctagt tggagctatg ggattggtag ttcttcctag
    39181 tgcagaggct gtagacccag ttgcgaccct agcgctggct agtcgagagg gtgttgttga
    39241 aatggatggc tatcgctatg ttggttatct atcaggtgac atcctcaaaa cgcttggctt
    39301 ggacactgtt ttagaagaaa cctcagcaaa acctggagag gtgactgtag tcgaagttga
    39361 gactcctcaa tcaacaacaa atcaggagca agctaggaca gaaaaccaag tagtagagac
    39421 agaggaagct ccaaaagaag aagcacctaa aacagaagaa agtccaaagg aagaaccaaa
    39481 atcggaggta aaacctactg acgacaccct tcctaaagta gaagagggga aagaagattc
    39541 agcagaacca gctccagttg aagaagtagg tggagaagtt gagtcaaaat cagaggaaaa
    39601 agtagcagtt aagccagaaa gtcaaccatc agacaaacca gctgaggaat caaaagttga
    39661 acaagcaggt gaaccagtcg cgccaagaga agacgaaaag gcaccagtcg agccagaaaa
    39721 gcaaccagaa gctcctgaag aagagaaggc tgtagaggaa acaccgaaac aagaagatac
    39781 acagccagaa gtagtagaaa caaaagatga ggctgctaat caacctgttg aagaaccaaa
    39841 agttgaaacg cctgctgtag aaaaacaaac ggaaccaaca gaggaaccaa aagttgaaca
    39901 agtaggtgaa ccagtcgagc caagagaaga cgaaaaggca ccagtctctc cagaaaagca
    39961 accagaagct cctgaagaag agaagactgc ggaagaaaca ccgaaacaag aagataaaat
    40021 aaagggtatt ggtactaaag aaccagttga taaaagtgag ttaaataatc aaattgataa
    40081 agctagttca gtttctccta ctgattattc tacagcaagt tacaatgctc ttggacctgt
    40141 tttagaaact gcaaaaggtg tctatgcttc agagcctgta aaacagcctg aggtaaatag
    40201 cgagacaaaa gctgaaaaag ttgcagctaa tacagatgct aaacaaagtg aagtaaattc
    40261 tgagactgca agtcttaaaa cagcaatttc tggattaaat acagataaag ttgaactcga
    40321 aaatcaactt aagattgcac agggaaaaac tgaaactgat ttcagcatgg aatcttggac
    40381 ggttttgagt acagctaaaa ataaagctca ggaagtaaaa gataacggaa cagcgaccca
    40441 agaacaaata aacgaagcag agaaaagttt gaaaacagcc cttgctgact tatctgttga
    40501 caaaacagct ttgggaagcg ctatcgatac agctaccaag aaaaataaag agaactatac
    40561 aaatcaaaca tgggcagagt tagaaacagt tcttacagca gcaaaatctg taaacactaa
    40621 cgagtctaaa caaagtgaag ttaatgaagc tgttgaaaaa ttaactgcaa ctattgaaaa
    40681 attggttgaa ttgtctgaaa agccacgttt gactctatct attgaaaaga gagacataga
    40741 tcgaaaagtt acggtcacgt atactttgga aaatccagca aatactcaaa taaaatcaat
    40801 aactgcaact ctgaaaaaag gagaggaagt tgttaaggat tttgttttaa cagaagaaaa
    40861 tcttaaaact aatcatttaa ctgctttgtt tgaaaagcta gattactaca aagaatacac
    40921 tctatctacg gatatggtat ataacagagg aaatgatgat gagacggaga gtatttcaga
    40981 agaactaatt cagctgaatc ttaagaaact agaattgaaa gatatccaga cagtctctct
    41041 gatgaaattt gaaaatggtc aagaaagtca ggttacacac ttgtctgata aaccgaccga
    41101 tttgagtaaa ttatacttaa aagttacatc atcaacgtct aaggatgctg tgttagccgt
    41161 tagctctata gaagaagaga ttgttgaaaa taaaaaaatc tttaaaattc atgcagatac
    41221 accagaatta gtagttcgta agaaagatgg cagtctttct aagggatttg actattacat
    41281 ggagagagtt atccctcatg atggtgatat ttattatgat tttaaagact tgatttcagc
    41341 tatgacatct aatcctacag gtacgtttat tttagggaga gatatttcct cgcgtaatgt
    41401 aaaaccggat ggaaatggaa aatcttatat caaaggtgaa tttaaaggaa aactacttgg
    41461 tacaaatgat aatgttagac actcgatctt tgacttagaa tatccattgt ttgatactat
    41521 aaaatcagga gtcgtgaaag atattgattt caagcatgtc aatatggtat tcccagatag
    41581 caatcaagga gataacgtag caacaattgc aagagtaatc aaagataaga caaaaattga
    41641 gaatgttaat gttgaaggtt atctcgaggg tcgagatcat gtagctggtt tagttaataa
    41701 tcttgaaggt aattccgaaa ttgaaaatat atcctttaca ggaaagatta agtctaaagg
    41761 tggtaattct attacagcag gtattgcagg tagaaatatc ttatctcgtg ttaaacgtgc
    41821 ctatgtaaat gcaaatattg aagtccttgg ttcaacgaat tcgagtatgc ttgttgcagt
    41881 aaatggtaca acattgaatg ctagtggagg ttggggagcc tggggaagac ttactgagtc
    41941 cgttgctaaa gggacgttag aaattaaacg cagtggacaa gctggtggag ttactgcaac
    42001 agtctggcct tatggagcaa ttgataaggt tgtcagctat gcaaaagtta ccaaaggaaa
    42061 agaattattt gggtcagatg gtgatttaaa taataattgg tttatgcaaa aaattaataa
    42121 tatttttggt gttcaaggta tcagctcagg tgactcaggg aatgattcta agtttaagcg
    42181 tatttccgag gaagaagcaa agcaaaaggt tgcctcttat aatattacag ctcccaactt
    42241 gatgagcgac tcaagtcttt tggtagacag actcaatgaa agttggaaaa atactgatca
    42301 atttgaaagt atacaggatt atcaatccca aaaccaatta atttaccaga acttgactaa
    42361 atttactcct tattacaata aagaatttat tgttcatgaa ggaaatgctc tcactcctga
    42421 acaggaaata ttgaagacta agaaaatcaa atcgattgta gggttgaaag gaacagagtt
    42481 cgttgtagat ggcagtgata ttgatactat catgcttcat tttgaggatg gtagtcagaa
    42541 gcgatataag gtaacctcta ctggtaagtt ttcgataact aatttaccgg aatatcaggt
    42601 tgaagattta aatgtggtgt atacatcaga acatatagta catccactag attcgtcact
    42661 gataaataac cttgttgaag aattgaaaaa agtagaattg tatacagagt ctacttacca
    42721 agttctaggt attgataaag ataatgctaa caaattgaat agaactaaga gattattctt
    42781 agatgagtca ttagatgctg ttaaaacaca attgcctacc tttgtaaaaa caatgtttga
    42841 aaatgagtgg ttgcacatca atggagaatc tagtggagct gtcgctgctt tacgacaaaa
    42901 aatcatggat aataagacgg ctatattgct tgctttgact tatatcaatc gttattatga
    42961 tgttaaattc tcagactata atattaaaaa actgatgctc tttaaaccaa ctttccatgg
    43021 tgaaaagatt gatctattag atcgattaat ccgactagga tcatctggag aaaatagatt
    43081 aaaaggttca gaaaatgcag aaacgttcaa gcagttattt gctagcgaaa cgaagcagaa
    43141 ggatttggtg acttatttag actacaatcg ctctttgttg actaattatc aaacaacagg
    43201 ggaatggttt aaagaaacga ctaaggatta tattcaattt gaggaacgtc catccttagt
    43261 agaggagatt aaagatgcca aatatcgagt ttacgataat ttaaccgcac cgtattatca
    43321 aggatatatc ctacctctct taacgttgaa gaatactcat ttagcaattt tatcgaacta
    43381 tagtactatg acatttgtca gccgtgaaaa acgccctaat tggaaaaatg aagattttga
    43441 taagtgggtg aaatatgtag caactgctca tagaaaccat gttgatactt ggtataagat
    43501 tttacctgat aatatcaaag gtaaaatggt taaagagaat gttactgctg tttgggaagg
    43561 attgagcatc ccaggaagtg aatgggtaga ccagaatgct gtagatagaa aaggaagaga
    43621 ttacgcccca gctcgagaat tctttaatct tgtaggtggt cctatgggag gttggtatgc
    43681 ttaccatggt tatggtgcgc atgctggagg aagaaatcgg gttaattatg aggtgtttga
    43741 tgtattaagt gagtatggta tatcggtctt tactcatgaa ttgactcacg ttaatgatac
    43801 ttggatttac ttaggagggt atggtagacg tgaaaatatg ggacctgaag cttatgctca
    43861 gggattattc cagtctcctg ttccaggtca gccgggttgg ggggcgcttg gtctcaatat
    43921 ggcatttgaa agaaaaaatg atggtgatct aatttataat gcctccccaa ctcaatttga
    43981 aaataggaaa gagttagata gttatatgaa aaattataac gatactctca tgatggtgga
    44041 ctacttggaa ggagatgcag ttatctctaa aggtaaagaa gctattacta aatggtttaa
    44101 aaaagttgaa cctaaggttg ttagtcaaac agctcagtat gatacggttc gtcagttgac
    44161 tgctgaagag aaagaaaaac tttctgtttc tagtgttgat gatttagttg atcaaggatt
    44221 aatgtcagac agagctgtag gaaataatac ctataatcca gctgattttg aaacaagtta
    44281 tattgcgata gattatatga ctggtattta tggtggagga aagaatagtg taggttcacc
    44341 aggtgccttg atgtttaaac ataatacttt ccgtatgtgg ggctactatg gttttgaaga
    44401 aggtgttcta ggatatgctt ctaataaatt taagcaagca tctagagatg aaggtcatgc
    44461 tggacttagt gataacttta ttatttctaa gatttccaaa ggggaatttt taactatgga
    44521 agcatttaaa aagggatatt ttaagaaagt agtagaagag ttaaaaacta aaggtattcg
    44581 tcctgttact atcaatcaaa aaacatatag cacctttgaa gaattgcaag aaggatttaa
    44641 gcaggctgtt gaaagagatt tgaagaaaaa tcagttagat gagagggaaa caagaaactt
    44701 caaattccaa gttttcagac aacttcttca acaaacagat agctttaaaa cttctatctt
    44761 tagataatcg aaatctctca tctgctttgc ggatgagggt tttcttattt tatgctatac
    44821 ttaaggtatg catagaaaaa cagtgattga ttttaggact ttaggggaga gatatacctt
    44881 tacccagcct attaaagagt tgaaaacgag aaatgtagaa gaagtggcag atttgctggc
    44941 acaagtggaa agctaccaag agcaaggtta ttatgtggtg gggtatgtca gctacgaggc
    45001 tgcacctgct tttgaggaaa aattagcagt tcacaaggat cccctactgg gagagtacct
    45061 gctttatttt actgttcatg atagcgtaga aacttcccct attcctctga cttatgagga
    45121 tattgatttg ccctcaaatt ggcaggaagt aacgtctgca gcagactatg aaaaggccat
    45181 tgcccagata caccatcact tgaggcaggg agacacctat caggtcaact acaccgtcca
    45241 actcaagcaa aagttaagtg ccaatccttt tgccatctac aatcgtatgg tggtagagca
    45301 ggaggcgggc tacaatgcct atgtggaaca tgacgagatg gcagtgattt ccatgagccc
    45361 agagctcttt tttgagcaaa atgatcgcga gttgacaaca cgaccaatga aggggacgac
    45421 tcagcgtggg gtaactgacc aagaagatct tgaacaggcc agttggttgg aacaggatcc
    45481 caaaaatcgc tctgaaaata tgatgattgt ggacctcttg cgcaatgata tgaaccgtat
    45541 ttctgaagtt gggagcgagc acgtggagcg tctgtgccag gtagagcagt attcaactgt
    45601 ttggcagatg acttcgacca tcaagagtca gttgcgagag gatgtggacc ttgttgaaat
    45661 cttccgctca ctctttcctt gtggttccat aacgggtgca ccgaaaattg cgacaatgga
    45721 gattatcaag aacttggagc ctcaaccgcg tggagtctac tgtggaacga ttggtctctt
    45781 gcttccaaat ggacgacgga tttttaatgt ggccattcgt accattcaac ttcacaaagg
    45841 tcaagccatt tatggagttg gcggagggat tacttgggat agcacatggg aatctgaata
    45901 ccgagaggtt catcaaaagg cagcagttct ttatcgtaaa caagctcgct tccaactgat
    45961 tacgacaggg gaaatcagcc agaagaacct gctgtttgaa gatcaacatc tggaaagact
    46021 gagaaaagct agtcgttatt ttgcctttcc ttttgatgca gaagacttgg gacacaagat
    46081 tgaggaagag tgtcaggatt gtgaagctaa tcaagattac cgcttgcgaa tcagccttag
    46141 caaatctgga gagatagaag tcaatcgtca agtattaacc cctctcagta caagcttttg
    46201 tcaggcccaa gtctgccttc aggaagctgc tttgaatcaa tcctttacct actttaaaac
    46261 cactcaccga ccgcatttga gcctaggaga acaagagaag atttaccaca ataagtcagg
    46321 agaactgctt gaaacctcta taggaaattt ggttctgaaa atcgctggaa aactctacac
    46381 accgcctatc cgacttggaa tcttgccagg aatttaccgt cagcatttgc tagaaacagg
    46441 acaggtagaa gagaaagtct tgaccttggc agatttaacc caagcagaag ctatttacgg
    46501 ctgtaatgca gtgagaggct tgtatgaact aagccttgag gagaactaga tgaaattgat
    46561 atttttgcat gggttagggc agtcagcaga gtcttggaaa gaagttcgga atctactgac
    46621 agattatcct tctgaggcta tcgagttatt tccttctgga gttagtaact atcaacaagc
    46681 taaagagcga gtttatcagc acctagctca agagacagaa ccttttgttt tgattggatt
    46741 gtccttaggt gctgccctga cactagaact ttccagttac gatttaccaa atcttcgagc
    46801 tttgattctg tcaggatgcc cgcttaaatt agcagggaat atcctatttt atcttcagtt
    46861 gctgattttt aaactacttc ccaaaagagt atttgaaaaa cagggagcag acaagactct
    46921 tatggtcgga gtttctgagg aattgaagac acttgattta acggatatag cagggacttg
    46981 ctcctatcca accttactaa tttgtggcag caaagataaa ccgaatctta gttttatgaa
    47041 agctctccat agactgttaa cagattcgca gtttcagatc attcctgatg gccctcatgt
    47101 cttgaataag gcaaaaccaa aggagtttgt agaaaagacc agaagtttcc ttgaattgct
    47161 gaaataagtt tgataaaata atattgaaat cgatatcata attatgggag aaagaaatga
    47221 ataaacgtct atttttaaaa atgagtctgg tgacgttgcc aattttagcc ttgttttcac
    47281 aaccagtttt ggcggaagaa aacatccatt tttcgagctg taaggaagct tgggcgaatg
    47341 gctattcgga tattcacgag agagaacctg gttattctgc caagttagac cgtgatcatg
    47401 atggtgtggc ttgcgaattg aaaaatgctc ctaagggtgc ttttaaagca aaacagtcaa
    47461 cggctattca aatcaacaca agttcagcaa caacaagtgg ttgggttaag caggacggcg
    47521 cttggtacta ctttgatgga aatggaaatc tagtgaaaaa tgcatggcag ggaagctatt
    47581 acctgaaagc tgatggtaaa atggcacaga gtgaatggat ttatgactct tcttatcaag
    47641 cttggtatta tttgaaatca gatggttctt atgcaaaaaa tgcatggcaa ggagcttatt
    47701 accttaaatc aaacggtaaa atggcacaag gtgagtgggt ttatgattct tcttaccaag
    47761 catggtatta cttgaaatca gatggttcat atgctcgcaa tgcatggcaa ggaaactact
    47821 atttgaaatc agatggtaaa atggctaaag gtgaatgggt ttatgatgcc acctatcaag
    47881 cttggtatta tttgacatca gatggttctt atgcttacag tacatggcaa ggaaattact
    47941 atctaaaatc ggatggtaaa atggctgtca atgaatgggt tgatggtgga cgttattatg
    48001 ttggcgctga cggagtttgg aaggaaggtc aagcaagtac agtttcttct agtaatgata
    48061 gcaatagtga atattctgct gctttaggaa aggcaaaaag ttataattcg ttattccaca
    48121 tgtcaaaaaa acgtatgtat agacaattaa cttctgattt tgataaattt tcaaatgatg
    48181 cagctcaata tgccattgat catttagatg attaacagat actggtaggc gaaaaaattc
    48241 cttataattc atttacgcaa acctttgcat aagtgaatgg attttgatat actagtactg
    48301 taagcatttt caattactac acaatgaaaa aggagaatat gatgagtcaa aagattattg
    48361 ggattgacct tggtggaact tctatcaaat ttgcaatctt aacaacagca ggagaaatcc
    48421 aaggaaaatg gtcaatcaag accaacattt tggatgaggg aagtcatatc gttgatgata
    48481 tgattgagtc tattcaacat cgtttggact tgcttggatt ggcagcagcg gacttccaag
    48541 gcattggaat gggatcacca ggtgtggttg accgtgacaa agggactgtt atcggtgcct
    48601 acaacttgaa ctggaaaacc cttcaaccaa tcaaacaaaa gattgaaaaa gctttgggca
    48661 ttccattttt catcgataat gatgccaacg tagcagctct tggtgagcgc tggatgggtg
    48721 ctggagataa ccaaccagac gttgtcttta tgacactcgg tactggtgtt ggtggtggta
    48781 ttgtcgcaga aggcaaattg cttcatggtg ttgctggtgc agcaggtgaa cttggtcata
    48841 tcactgttga ctttgaccaa ccaatctcat gtacttgtgg taaaaaaggc tgccttgaga
    48901 cagttgcttc agcaacaggg attgtcaact tgactcgtcg ctatgccgat gaatacgaag
    48961 gcgatgcagc cttgaaacgc ttgattgata acggagaaga agtaactgct aagactgtct
    49021 ttgatctcgc aaaagaagga gacgaccttg ctttgattgt ttatcgtaac ttctcacgtt
    49081 acttgggaat cgcttgtgct aacattggtt caatcctaaa cccatcaaca atcgtcatcg
    49141 gtggtggtgt gtcagctgcg ggagaattcc ttctacaagg tgttcaaaaa gtttacgatg
    49201 aaaatagttt cccacaagta cgcacatcta ctaaattagc tcttgcaact ctaggaaatg
    49261 acgctggagt tatcggagca gcatcacttg tattgcaata agataatgag agaggaaaat
    49321 gctaacctag agttagtaat gttcctcttt tattttttat gctatactag ttaggacaat
    49381 gaatgaggtg agagtaaatg acaaaagcag atacgatttt taaagagaat attgaacgca
    49441 ttctaaagga aggtgttttc tcagagcagg ctcgtcctaa gtacaaggat gggactgttg
    49501 ctaattccaa gtatgtaact ggtgcctttt ccgagtacga cttatctaaa ggagaatttc
    49561 ctatcacaac cttgcgtccc attgccatca aatccgccat caaagaagtt ctctggattt
    49621 accaagacca gtcaaatagc ttagaagtgc ttaatgacaa gtacaatgtt cactactgga
    49681 atgactggga agttggagac acgggaacca ttggtgagcg ctatggtgcc gttgttaaga
    49741 aacacgacat tatcaataag cttctcaaac agttggaaac caatccttgg aaccgccgca
    49801 atattatttc gctctgggat taccaagctt tcgaagaaac agatgggctg ctcccgtgcg
    49861 cctttcagac catgtttgat gtccgccgta tagatggaga aatctatctg gatgcgacct
    49921 tgacccagcg ctcgaatgat atgctggtgg cccaccacat caacgctatg cagtatgtgg
    49981 ctttgcagat gatgattgcc aaacattttg gctggaaggt tgggaagttc ttctacttca
    50041 tcaacaacct ccatatctat gataatcaat ttgaacaagc tcaggaattg ctccgtcggg
    50101 agccgtcaaa ctgccaacca cgcttggttt taaatgttcc tgatgggact aatttctttg
    50161 atatcaaagc agaagatttt gagttggtgg attatgaccc tgttaagcca cagttgaagt
    50221 ttgacctagc tatttaaaag aatagaaaaa agaagttgag aataatccca acttcttttg
    50281 tttcttaacg tgatacgcgg cgacgagctg cttttttacg gttttcttcg atgaaagctg
    50341 ctttttgctc ttctggttcg attactttct ttttaaatgc gtatactgca cctgcaacgg
    50401 cagcgacagt tcctgcgaca cctgttacaa gacctttagc gaatccttta gccatgagtc
    50461 ttcctccttt atattctcaa tcagccagcc tcctcaagag gtcacatttt tctgactgac
    50521 ctttttgtgt tataataata gtaacgaaaa aatgggaatt tttcaaggaa aaaagatgaa
    50581 aacaaaaata attgtgattg ttggaccgac tgctgttgga aagacggctc tagctattga
    50641 agttgcaaag cgttttaatg gcgaagtggt tagtggagat agccagcaag tctatcgagg
    50701 acttgatatt gggacggcca aggctagtcc agaagagcag gcagctgttc ctcatcattt
    50761 aatcgatgtt agagagataa ccgagtctta ctcggctttt gattttgttt cagaagctaa
    50821 gatgactatt gagggtattc acaaccgtgg caagctagcc attatcgccg gtgggactgg
    50881 actttatatc cagagcttgc tagaaggtta ccacctaggt ggggagactc ctcatgagga
    50941 gattttagct tatcgagcta gtttggagcc atattcagat gaggaattag cccatttggt
    51001 ggatcaagca ggccttgaga ttccccagtt taatcgtcgt cgtgctatgc gtgccttaga
    51061 aattgcccat tttggtcagg atttggaaaa tcaagagacc ttgtatgaac cgctgattat
    51121 ctgcttggat gatgaacgta gtcaacttta tgagcgtatc aaccaccgag tggatttgat
    51181 gtttgaggct gggcttttgg atgaggccaa gtggctgttt gaccattccc ctaatgtaca
    51241 ggctgctaaa ggaattggct ataaggaact ctttccttat ttccgtggag agcagacctt
    51301 ggaggaagct agtgagagtc tcaaacaggc gacccgtcgt tttgccaagc gtcagctgac
    51361 ctggttccgt aatcgcatgc aggtcacctt ttatcagatt ggagaatctg gtgtacaaga
    51421 ccgcatttta agccagatag aggagttttt agatgattga aacggagaaa aaagaggagc
    51481 gagtcctgct gattggtgtg gaattgcagg gtatggacag ttttgacctc tccatggaag
    51541 aattggctag tttagcgaaa acggcagggg cagtcgttgt agatagctac agacaaaaac
    51601 gtgaaaaata tgattccaag accttcgtcg gctctggtaa gttggaagag attgcgctta
    51661 tggtggatgc agaagaaatc actactgtca tcgtcaacaa ccgtctgacc ccaaggcaga
    51721 atgtcaatct agaggaagtt ctcggtgtta aggtcattga ccgtatgcag ttgattttgg
    51781 atatctttgc catgcgggct cgaagccatg aagggaagct ccaagtccac ctagcccaac
    51841 tcaaatacct tttgcctcgc ttggttggtc aggggattat gctcagccgt caggcagggg
    51901 gaattggttc ccgtggtcct ggtgaaagcc agctggagct gaaccgtcgt agcgttcgca
    51961 atcaaatcac ggatattgag cgccagctca aggtggttga gaaaaatcgg gcgactgtca
    52021 gagaaaaacg tttggagtct agcactttta agattggttt gattggttat actaatgctg
    52081 ggaaatcaac tatcatgaac atcttgacca gtaagaccca gtatgaagca gatgagctct
    52141 ttgcgactct ggatgcgaca accaagagta ttcatctggg aggcaatctc caagtaactt
    52201 tgacagatac cgttggcttt atccaagatt tgccgacaga gttggtgtcc agtttcaagt
    52261 caaccttgga agaaagcaag catgtggacc ttctggttca tgttatcgat gctagcaatc
    52321 cttaccacga ggagcatgaa aaaacggttc tctccatcat gaaagacctg gacatggaag
    52381 atattcctca cttgacgctt tataataaag cggatttggt ggaggatttc acgcctaccc
    52441 aaacgccata taccctcatt tctgccaagt ctgaggacag tcgtgaaaac ttgcaagcat
    52501 tattgctaga taagattaag gaaatttttg aagcatttac cctgcgagtg cctttttcaa
    52561 agtcctacaa gattcatgat ttagagagtg ttgcaattct ggaagaacgt gattatcagg
    52621 aagacggcga agtgattaca ggctacattt ccgagaaaaa taaatggagg ttagaagaat
    52681 tttatgactg atattaaaac tttggctcta aagtatggag gttatacaag tctggacaag
    52741 gtctatctgg atcagcttct agctggtaaa acagagcagg agcagttaac acttattaca
    52801 cctccgccaa gtgttgttaa tgcctacttt gcagaactct atcagaaaaa aagtcctcag
    52861 gctgcgacgg attattttgc agaactcagt caggaactga atctttacaa tactgagcca
    52921 agtttcaccc tagaaaataa gccttttatt cggcttaatc tgtctggtaa atcctttggt
    52981 ttttgttatg agagtgacgg ctttggtcgg attttctctg aaaatgagga gaaaatctcg
    53041 gatgacttgc tctttgaaat tgcgcaaatt ttctcccatc aattagtctt tgaagagtct
    53101 ggtaagattt acatgaagcc tgtcaaggac gaggaagttg tcagcctaga aagtctcaca
    53161 gctttgactg atttggaaag attggctgat ggtcgaaaac gtctcaaagg ctacagccaa
    53221 gaggatttat tgcaagaagc tgctgctttt tctggcaaac gctatttccg atcggaaaac
    53281 cgcacagcca tgttatatat tgattaatta gaaagtatcg aatggatatt caatttttag
    53341 gaacgggggc tggtcagccc tctaaagccc gcaacgtttc aagtctcgcc ctgaaacttt
    53401 tggacgagat taacgaagtt tggctctttg actgtggaga aggtacgcaa aatcgcattc
    53461 tggaaaccac aattcgacca cgtaaggtca gcaaaatctt tattactcat ctgcatggag
    53521 accacatttt tggtttgcca ggtttccttt ctagccgtgc ctttcaggcc aatgaagagc
    53581 agacagattt ggaaatctac ggacctcaag gaatcaagtc atttgtctta accagccttc
    53641 gtgtgtcagg ttctcgtctg ccctaccgca ttcatttcca tgagtttgac caagattctc
    53701 tgggtaaaat tcttgaaacc gataaattca ctgtgtatgc agaggagctg gaccacacta
    53761 ttttctgtgt tggctatcgt gtcatgcaaa aggatctaga agggacgctg gatgctgaaa
    53821 aactcaaggc tgctggtgtt ccgttcggcc cgctttttgg taaaatcaaa aacggccagg
    53881 atcttgtttt ggaagacgga actgaaatca aggcagcaga ctatatctca gcgccacgtc
    53941 caggtaagat tatcactatt ttaggagaca ctcgaaaaac ggatgccagt gtgcgtctgg
    54001 ctgtcaatgc agatgtccta gttcatgagt ccacttatgg caagggtgat gaaaaaattg
    54061 ctcgtaacca tggtcactca actaatatgc aagctgcaca agtagcggta gaagcaggtg
    54121 ccaaacgcct cctactcaac catatcagtg cccgtttcct ctcaaaagat ataagcaaac
    54181 tcaagaagga cgctgccaca atttttgaaa atgtccatgt ggtcaaagac ttggaagaag
    54241 tggaaatcta gcagtcacag aaaggataag tatgcctact attctcatta ccggagctag
    54301 cggtggtcta gctcaagaaa tggtcaaact cctgcccaat gaccaactca tcttgcttgg
    54361 tagaaataag gaaaaattag cccaactcta cggaaattat tcccatgcag aattgattga
    54421 aattgatatt accgacgatt cagccctaga agctctggta actgatcttt atctccgcta
    54481 tggcaagatt gatgtcttga ttaacaacgc tggttacggg atttttgagg gatttgacca
    54541 gattgctgat aaagatattc accagatgtt tgaggtcaat acctttgccc tgatgaatct
    54601 gtctcgtcac cttgcggctc gtatgaagga aagcagcaaa gggcatatca tcaacatcgt
    54661 cagcatggca ggtctaatag ctactggcaa gtctagtctt tactcagcga ccaagtttgc
    54721 ggctattggt ttttcaaatg ctctgcgact cgaacttatg ccctatggag tctatgtgac
    54781 aacagtcaat ccaggaccaa tccgtacagg atttttcgac caagccgacc cagatggaac
    54841 ttatcttaaa tcggttgacc gcttcctgct agaaccagat gcagtggcta aaaagattgt
    54901 caagattata ggaaaaaaca aacgagaact caatctcccg attttgttga acctagctca
    54961 taagttttat accctctttc ctaagctagc tgataagttg gcaggggaaa cttttaatta
    55021 taagtaaaaa gaaccaatgt gcaggttgtt gctagcctac atattggttc ttatcatttc
    55081 tcaactatca aactgaactt cttctaggag gtagtctata aagcgttcgc ccatcttaga
    55141 taggctggtt ttctcatgct ggatatagac cagctcaatc gggtcatcaa tatccagtgg
    55201 gatagaaacg atattgtctc cgtttaggtt gctgttcaaa atccctgtcg caatggtata
    55261 accatccaaa ccaatcaaga gattaaagag ggtggcacgg tcactgacca caatggattt
    55321 cttgtggtgt tcttgagaaa gaatctcttc tgaaaagtag aaggagttgt gcgtcccttg
    55381 gtcatagctg aggtaaggga aattctccaa atcagacagt ttcaccttgt ctttctttgc
    55441 cagagggttg gtcttgctga caaagatatg tggttgcgct gtgaagagat ggtgggctag
    55501 caggtgattg tcatccagca tcttggttaa aacatcacgg ttgtaactgt ttaagaagag
    55561 gaccccgacc tcactgcgga agttcttgac gtcgtcgata atctcccaag tccgagtttc
    55621 acgaaggaag agttcgtatt tctccatatc gcttttcttg agcaaagaga caaaggcatt
    55681 gaccacaaag gcatagtgtt gagacgaaac gctaaatagt tcgcggtggg cgacaggatt
    55741 tttatagcgt tcctccagaa gctgggtctg ctcgacaacc tgacgggcat aggagagaaa
    55801 ctccatccca tcacgggtca aggtgattcc cttgggattg cggataaaga tctcaatgcc
    55861 catttcattt tccaaatctc gcactgcatt ggagagactt ggctgagtga taaagagttg
    55921 cttggctgcc tcattcatgg agccagtttc gacgattttg ataatataat gtaattgttg
    55981 aattctcatg tttttattgt accatacttg ccttagaatc gctaaagaag atgggaaaat
    56041 aaggagcatt gtgtgagtct cttcttgaaa aaaacacaaa attttgctag aatgagtctt
    56101 atgaaaacat tctatgatgt gcagcaattc ctcaaacgat ttggtattat tgtttacatg
    56161 ggaaaacgct tatatgatat tgaactgatg aagttggaac tatctcggat ttacgatgca
    56221 gggttgatgg ataaattaga ctatctagag gcagaagcag ttcttcgcag agagcacaag
    56281 gtagaattgg attatattga gaaaaatgga gaaaagaact aatggttact tggattttgt
    56341 gggcacttat actagcaatg ttggcgtgga tgggctttaa ctatcttcgt attcgccgtg
    56401 cggctaaaat tgtggacaat gaggagtttg aagccttgat tcgtacgggt caattgattg
    56461 atttgcgcga cccagcagaa ttccacagaa aacatatcct tggtgcacgc aatattcctt
    56521 caagtcagtt gaaaactagt cttgcagccc ttcgtaaaga taaacctgtc cttctctacg
    56581 aaaaccaacg tgcgcaacga gttacaaatg cagctcttta cttgaaaaaa caaggttttt
    56641 ctgagattta tatcctttct tatggcttgg attcttggaa agggaaagtg aagactagct
    56701 aaccttattt ccaaattagt tgaatgatag gatgaagcta ttgttttata gtataattaa
    56761 tttgtataaa atttaaggag ttcttagaac atgaaagaaa agaagaaaat ccactttttg
    56821 tgggtatctt gtcgattatt cttggtttgt tatttccaat tgtaggcctg attttgggta
    56881 tcataggatt ggtcttggct atttcatacc aaaaagagtc acaattagac tataaaatag
    56941 aaaagattct taatatctta ggaattgtga tttctgtagt taactggatt gtagcaatcg
    57001 ccttaatttt tcggtaaata aaagaaaaaa gctttagttt taaagctttt tttattttat
    57061 cgactcatct ctaaatagtt tttaatgatt tgtaactctt tttggttcaa agggcggtag
    57121 ttaccttctg ctaaatctgg gttcaatgtg aagtccccaa attggattct cttgaggcta
    57181 gtcaccttaa caccaaccga gaggaacatt ttcttgattt gatgaaattt tccttctgaa
    57241 atggtgatag aggcttgact gatggaggga cttgcagata gaatctctag ttttgcgggt
    57301 ttacagacag taccatctaa aaagacaatt cccttttgaa aggcttggat atggtcaggt
    57361 gttagaagtc cattaacctc aacttgataa gtcttatcga catgatattg gggatggagg
    57421 agttggaagc ccaagggacc gttatcggtc aagagaagga gtcctgttgt atctcggtcc
    57481 agtcggccga cggcatagag cttgtcagac tggatgtcag atggaagcag gtccatgacg
    57541 gtcggaagtt ccttgtcttt gttggctgta acgacaccag caggtttatg aagcataaga
    57601 taggtgtgtt catagccttg aatgattcga tcctgaaaaa ggagttcttg tagtcctgta
    57661 tcgatatttt gagctaggga gcgggctggg caaccatcga ctagaatttc ccctctgagg
    57721 agtgcttgtt tcatggcctt tcggctgatt ttttcttgag ctaataaatt atctaaacgc
    57781 atactgtgcc tatcttatca taagtcgctt gctttgaaaa ggcttgacgt gaaaagaaaa
    57841 gcggagtgaa aacatttaca cttgcttgag ttatgttatt tatttgaaat tatggtataa
    57901 tcgttcagtt agaaaataaa ttttgaatat tatagaggaa atcatgacaa aattaagaga
    57961 agatatccgt aacattgcga ttatcgccca cgttgaccac ggtaaaacaa ccctcgttga
    58021 cgaattattg aaacaatcag aaacgcttga tgcacgtact gaattggcag agcgtgctat
    58081 ggactcaaac gatatcgaaa aagagcgtgg gattaccatc cttgctaaaa atactgccgt
    58141 tgcttacaac ggaactcgta tcaacattat ggacacacca ggacacgcgg actttggtgg
    58201 agaagttgag cgtatcatga aaatggttga cggtgttgtc ttggtcgtag atgcctatga
    58261 aggaaccatg ccacaaactc gtttcgtatt gaaaaaagcc ttggaacaag accttgtccc
    58321 aatcgtggtt gttaacaaaa tcgataagcc atcagctcgt ccagtagaag tagtggatga
    58381 agtcttggaa cttttcatcg agcttggtgc agatgacgac cagcttgatt tcccagtggt
    58441 ttatgcttca gcgatcaacg gaacttcttc attgtcagat gatccagctg accaagaagc
    58501 gactatggca ccaatctttg acacgattat cgaccatatc ccagctccag tagataactc
    58561 agatgagcct ttgcagttcc aagtgtcgct tttggactac aatgacttcg ttggacgtat
    58621 cggtatcggt cgtgtcttcc gtggtacagt taaggttggg gaccaagtta ccctttctaa
    58681 acttgacggt acaactaaaa acttccgtgt tacaaaactc ttcggtttct ttggtttgga
    58741 acgtcgtgaa atccaagaag ccaaagcggg tgacttgatt gccgtttcag gtatggaaga
    58801 catctttgtc ggtgaaacca tcactccgac agatgcagta gaagctcttc caatcctaca
    58861 catcgatgag ccaactcttc aaatgacttt cttggtcaac aactcaccat ttgctggtaa
    58921 agaaggtaaa tgggtaacct ctcgtaaggt ggaagaacgc ttgcaggcag aattgcaaac
    58981 agacgtttcc cttcgtgttg acccaactga ttcaccagat aaatggactg tttcaggacg
    59041 tggagaattg cacttgtcaa tccttatcga aacaatgcgt cgtgagggct atgaacttca
    59101 agtatctcgt ccagaagtta tcgtaaaaga aatcgacggt gttaaatgtg aaccatttga
    59161 acgtgtacaa atcgacactc cagaagaata ccaagggtct gttatccaaa gcctttctga
    59221 acgtaaaggt gaaatgttgg atatgatttc aactggtaat ggtcaaactc gtttggtctt
    59281 ccttgttcca gcgcgtggtt tgattggata ctcaactgag ttcttgtcaa tgactcgtgg
    59341 ttacggtatc atgaaccata ccttcgacca atacttgcca ttgattccag gggaaattgg
    59401 tggacgtcac cgtggtgccc ttgtttctat cgatgctggt aaggctacaa cttactcaat
    59461 catgtctatc gaagaacgtg gtacgatctt tgtcaaccca ggtactgagg tttatgaagg
    59521 aatgatcatc ggtgaaaact ctcgtgaaaa tgacttgaca gttaacatca cgaaggcaaa
    59581 acaaatgacc aacgttcgtt cagctactaa ggaccaaaca gctgttatca agacgcctcg
    59641 tatcttgaca cttgaagagt ctcttgagtt cttgaacgac gatgagtaca tggaagtaac
    59701 gcctgagtct atccgtttgc gtaaacaaat ccttaacaaa gcagagcgtg agaaagctaa
    59761 caagaagaaa aaatcagctg aataagagct agaaagagag aaagatggtc tatttagtcc
    59821 taggaatttt actgctccta ctctatgtat ttgcgacacc agaaagcatt aaagggactg
    59881 tcaatatcgt cgctatggta tgtattttag tggcactctt gattttattg gttctatctt
    59941 ttctgaaaat ttttcaatta ccaacagaaa tattcctagc aatagccatg ttgatcctag
    60001 cttactttag tgttagagac atcacactca tgccagtcaa aaaaagtaaa agaagataaa
    60061 aaagagagtt tcggctctct ttttgctagg aattttacaa tagtttgaat tgctttttag
    60121 ttaacaccat ctcttttact tgtatagtat gaatgggaaa aacttgaata aaattcgtta
    60181 atggcatcta ttttatattt ggttagtact ggttgcatat tattttga
//
LOCUS       DAVKMU010000005        56927 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKMU010000005 DAVKMU010000000
VERSION     DAVKMU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.005"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..137)
                     /locus_tag="VW343_000374"
     CDS             complement(<1..137)
                     /locus_tag="VW343_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9754990.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     G"
     gene            307..378
                     /locus_tag="VW343_000375"
     tRNA            307..378
                     /locus_tag="VW343_000375"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:340..342,aa:Glu,seq:ttc)
     gene            384..457
                     /locus_tag="VW343_000376"
     tRNA            384..457
                     /locus_tag="VW343_000376"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:418..420,aa:Asn,seq:gtt)
     gene            complement(498..1040)
                     /locus_tag="VW343_000377"
     CDS             complement(498..1040)
                     /locus_tag="VW343_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9754991.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(1300..2553)
                     /locus_tag="VW343_000378"
     CDS             complement(1300..2553)
                     /locus_tag="VW343_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9754992.1"
                     /translation="MKCFVGLDISSTKLDVCIMLSDTSTPVTASLSNDLSGATEIKNH
                     ILDLNHTYHFERIVIGMEATSLYSFHPAMFFHEDRQLKELHVEIMVEQPNKIKKYRDA
                     FEENKNDTIDAFYIADYFRIERFSPSFLKEEKYMALQHLTRTRLQIIEQLTRTKQHFI
                     ENIYYKCNTLAYQFKKEELSTNLWSSTMMTLMTDEMTLDELANMPLQELDDWLQKLGK
                     GHFKDSEKLAQTIQAAIRGSYRLSKLQQDSVNVILGLLAREIRNLEKMIKEIDKAIED
                     MVETIPEYQCLTSIPGVGKVYAAGIIAEIGQIERFKDHPQVAKFAGLNWREKQSGNSN
                     SQHTSLVNRGNRYLRYYLVEAANSVRRYDDEYKDFYKKKYHEVPKHQHKRAILLTARK
                     FVRLVDVLLRNHQLYTPPRRIMEDN"
     gene            2909..4667
                     /locus_tag="VW343_000379"
                     /pseudo
     CDS             2909..4667
                     /locus_tag="VW343_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(4697..7240)
                     /locus_tag="VW343_000380"
     CDS             complement(4697..7240)
                     /locus_tag="VW343_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9754993.1"
                     /translation="MKSFLKTYRTYFISLIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLSPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYTLMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVLFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSTYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVDFDY
                     FSPIPYEKIENTNNLISVTSSSNEDAAIQYQIEVPENSQIYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(7302..8924)
                     /locus_tag="VW343_000381"
     CDS             complement(7302..8924)
                     /locus_tag="VW343_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9754994.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            9130..10155
                     /gene="trpS"
                     /locus_tag="VW343_000382"
     CDS             9130..10155
                     /gene="trpS"
                     /locus_tag="VW343_000382"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9754995.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            10307..11785
                     /gene="guaB"
                     /locus_tag="VW343_000383"
     CDS             10307..11785
                     /gene="guaB"
                     /locus_tag="VW343_000383"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9754996.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(11836..12933)
                     /gene="recF"
                     /locus_tag="VW343_000384"
     CDS             complement(11836..12933)
                     /gene="recF"
                     /locus_tag="VW343_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9754997.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(12936..13304)
                     /gene="yaaA"
                     /locus_tag="VW343_000385"
     CDS             complement(12936..13304)
                     /gene="yaaA"
                     /locus_tag="VW343_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9754998.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            13463..14713
                     /locus_tag="VW343_000386"
     CDS             13463..14713
                     /locus_tag="VW343_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9754999.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            14710..15993
                     /locus_tag="VW343_000387"
     CDS             14710..15993
                     /locus_tag="VW343_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9755000.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGYVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTIDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            16035..16856
                     /locus_tag="VW343_000388"
     CDS             16035..16856
                     /locus_tag="VW343_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9755001.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            16867..17412
                     /gene="pgsA"
                     /locus_tag="VW343_000389"
     CDS             16867..17412
                     /gene="pgsA"
                     /locus_tag="VW343_000389"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9755002.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            17409..18236
                     /locus_tag="VW343_000390"
     CDS             17409..18236
                     /locus_tag="VW343_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9755003.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            18221..19060
                     /locus_tag="VW343_000391"
     CDS             18221..19060
                     /locus_tag="VW343_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9755004.1"
                     /translation="MGIALENVNFIYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            19053..19847
                     /locus_tag="VW343_000392"
     CDS             19053..19847
                     /locus_tag="VW343_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9755005.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            19906..20724
                     /gene="mreC"
                     /locus_tag="VW343_000393"
     CDS             19906..20724
                     /gene="mreC"
                     /locus_tag="VW343_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9755006.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            20724..21218
                     /gene="mreD"
                     /locus_tag="VW343_000394"
     CDS             20724..21218
                     /gene="mreD"
                     /locus_tag="VW343_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9755007.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            21312..22490
                     /locus_tag="VW343_000395"
     CDS             21312..22490
                     /locus_tag="VW343_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9755008.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            22714..23493
                     /gene="rpsB"
                     /locus_tag="VW343_000396"
     CDS             22714..23493
                     /gene="rpsB"
                     /locus_tag="VW343_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9755009.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNK"
     gene            23559..24599
                     /locus_tag="VW343_000397"
     CDS             23559..24599
                     /locus_tag="VW343_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9755010.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            24845..25168
                     /gene="tnpB"
                     /locus_tag="VW343_000398"
     CDS             24845..25168
                     /gene="tnpB"
                     /locus_tag="VW343_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9755011.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWSSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(25309..25596)
                     /locus_tag="VW343_000399"
                     /pseudo
     CDS             complement(25309..25596)
                     /locus_tag="VW343_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            25587..25985
                     /locus_tag="VW343_000400"
     CDS             25587..25985
                     /locus_tag="VW343_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9755012.1"
                     /translation="MKAPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(26182..27102)
                     /gene="cysK"
                     /locus_tag="VW343_000401"
     CDS             complement(26182..27102)
                     /gene="cysK"
                     /locus_tag="VW343_000401"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9755013.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(27387..28022)
                     /locus_tag="VW343_000402"
     CDS             complement(27387..28022)
                     /locus_tag="VW343_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9755014.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            28078..29376
                     /locus_tag="VW343_000403"
     CDS             28078..29376
                     /locus_tag="VW343_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9755015.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            29373..30035
                     /locus_tag="VW343_000404"
     CDS             29373..30035
                     /locus_tag="VW343_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9755016.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            30115..30663
                     /gene="raiA"
                     /locus_tag="VW343_000405"
     CDS             30115..30663
                     /gene="raiA"
                     /locus_tag="VW343_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9755017.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            30799..32772
                     /locus_tag="VW343_000406"
     CDS             30799..32772
                     /locus_tag="VW343_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9755018.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            32769..33221
                     /gene="rplI"
                     /locus_tag="VW343_000407"
     CDS             32769..33221
                     /gene="rplI"
                     /locus_tag="VW343_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9755019.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            33265..34617
                     /gene="dnaB"
                     /locus_tag="VW343_000408"
     CDS             33265..34617
                     /gene="dnaB"
                     /locus_tag="VW343_000408"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9755020.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            34619..34885
                     /locus_tag="VW343_000409"
     CDS             34619..34885
                     /locus_tag="VW343_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755021.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            34974..36320
                     /gene="cbpD"
                     /locus_tag="VW343_000410"
     CDS             34974..36320
                     /gene="cbpD"
                     /locus_tag="VW343_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9755022.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      36403..36505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            36576..36866
                     /locus_tag="VW343_000411"
     CDS             36576..36866
                     /locus_tag="VW343_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9755023.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQGADNVFSNVKINVGEILSIDEKLEKYTETTH"
     gene            36829..37587
                     /locus_tag="VW343_000412"
     CDS             36829..37587
                     /locus_tag="VW343_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755024.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWFNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            37625..38632
                     /locus_tag="VW343_000413"
     CDS             37625..38632
                     /locus_tag="VW343_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9755025.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            38632..39360
                     /locus_tag="VW343_000414"
     CDS             38632..39360
                     /locus_tag="VW343_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755026.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPQGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            39479..39937
                     /locus_tag="VW343_000415"
     CDS             39479..39937
                     /locus_tag="VW343_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9755027.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            39939..42371
                     /locus_tag="VW343_000416"
     CDS             39939..42371
                     /locus_tag="VW343_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9755028.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIATLRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            42692..43345
                     /gene="rr06"
                     /locus_tag="VW343_000417"
     CDS             42692..43345
                     /gene="rr06"
                     /locus_tag="VW343_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9755029.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            43342..44673
                     /gene="hk06"
                     /locus_tag="VW343_000418"
     CDS             43342..44673
                     /gene="hk06"
                     /locus_tag="VW343_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9755030.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            44768..45295
                     /locus_tag="VW343_000419"
     CDS             44768..45295
                     /locus_tag="VW343_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9755031.1"
                     /translation="MVLAIILVIFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            45374..47353
                     /gene="pspC"
                     /locus_tag="VW343_000420"
     CDS             45374..47353
                     /gene="pspC"
                     /locus_tag="VW343_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU9755032.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVAVASLVMGSVVHATEKEVTT
                     QVPTYSNMAKTEHRKAAKQVVDEYIEKMLREIQLDRRKHTQNFAFNMKLSAIKTEYLY
                     GLKEKSEAELPSSEAELPSEVKAKLDAAFEQFKKDTLKLGEKVAEAEKKVAEAEKKAK
                     AQKEEDRRNYPTITYKTLDLEIAESDVEVKKAELELLKEEAKTRNKDTIKQAKAKVES
                     KQAEATRLEKIKTDRKKAEEEAKRKAAEEDKVKDKLKRRTKRAVPGEPATPDKKENGA
                     KSSDSSVGEETLPSPSLKPEKKVAEAEKKVAEAEKKAKDQKEEDRRNYPTNTYKTLEL
                     EIAESDVKVKEAELELVKEEAKESRNEEKVKQAKAKVESKQAEATRLEKIKTDRKKAE
                     EEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPAPKPENPAEQPKAEKPADQQAEED
                     YARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNG
                     SWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMATGWLQY
                     NGSWYYLNANGDMATGWLQNNGSWYYLNANGDMATGWVKDGDTWYYLEASGAMKASQW
                     FKVSDKWYYVNGSGALAVNTTVDGYGVNANGEWVN"
     gene            complement(47583..48563)
                     /gene="dusB"
                     /locus_tag="VW343_000421"
     CDS             complement(47583..48563)
                     /gene="dusB"
                     /locus_tag="VW343_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9755033.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(48550..49422)
                     /gene="hslO"
                     /locus_tag="VW343_000422"
     CDS             complement(48550..49422)
                     /gene="hslO"
                     /locus_tag="VW343_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9755034.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            49587..51014
                     /locus_tag="VW343_000423"
     CDS             49587..51014
                     /locus_tag="VW343_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9755035.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKLERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            51173..52681
                     /gene="glpK"
                     /locus_tag="VW343_000424"
     CDS             51173..52681
                     /gene="glpK"
                     /locus_tag="VW343_000424"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9755036.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            52723..54549
                     /gene="glpO"
                     /locus_tag="VW343_000425"
     CDS             52723..54549
                     /gene="glpO"
                     /locus_tag="VW343_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9755037.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            54619..55323
                     /locus_tag="VW343_000426"
     CDS             54619..55323
                     /locus_tag="VW343_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9755038.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            55620..55823
                     /locus_tag="VW343_000427"
     CDS             55620..55823
                     /locus_tag="VW343_000427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755039.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(55910..56518)
                     /locus_tag="VW343_000428"
     CDS             complement(55910..56518)
                     /locus_tag="VW343_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755040.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT        17698 a         9710 c        12348 g        17171 t
ORIGIN      
        1 tttcctttct gttgataggc tagtaacaga cgattgattt gtctttcaga aagattgagt
       61 tcgacacagg cccgtttctt tgttttcttt ccttgggcta tagcttttat cacaagatat
      121 tttttcgttt cattcatatt cagttggatc cttttcatat gactattcta ccaaatggga
      181 cattttcacg ttcgatttac taaagacatt atcacattcg aattacatat tttagagaaa
      241 aaatctcaaa agtgattgac aattagcaag aaattgatat aatggttata tctgctacag
      301 atagaaggtc cgttggtcaa ggggttaaga caccgccttt tcacggcggt aacacgggtt
      361 cgaatcccgt acggactatt ttatccgcca tagctcagtt ggtagtagcg catgactgtt
      421 aatcatgatg tcgtaggttc gagtcctact ggcggagtat agataaaagg gaacacagct
      481 gtgttcctct ttttgtatca atttgtatca ccaagcattt tcataaggaa gtctgttatt
      541 tcttgagaac tttctttttt tccatgtgca atccaagttt ggcagacacc aaaaagtgca
      601 tgagttagat agatgctact atattctaat tcagtggtat ttagattcag ttgcataaat
      661 cgcttttgta aatctgtact aagcatgata tgaagtttat ttcgtaagaa attttggatt
      721 tctttagtcc cattttcaga aagaagggca gccagaagtg gttctgactc tagatattca
      781 aaaacttcta aaatagcgtc tcttttgtga tgagcatgtt tttgaaaaat atattcaaat
      841 gtatggaata gcttgctttg atagtgctca atcatatcat acttatcctt atagtgagta
      901 tagaagctgg aacgactaat tccggctttt tctactaatt tgacagtaga aattttatca
      961 aatggctgtt ccatcagtaa ttgtaccata gcgttttcaa tagttcgctt tgtttttaag
     1021 cgtttgttac tttcttgcat atttcctcct tgtaaacaaa ttagactata tgtctaaaaa
     1081 tagatttttt atcttgtaat ttagattttt taatgtataa tctattatat caaaatttta
     1141 gacaatatgt ttaaaaaagt ctagtggtaa aatgtcaagc gaccccccgc tcaaagtttg
     1201 aaaactattt caaaaaaagg gtgcactaaa gaagccctac tgaaaacgat gttttcacac
     1261 tgtttttagt agattttcaa ggagaggtgc agatataact tagttatctt ccataatcct
     1321 ccttggtggc gtatagagtt gatggttgcg tagtagcaca tccaccagac gcacaaattt
     1381 tctagcggtt agtaagatgg ctcgtttgtg ttggtgttta ggtacttcat ggtatttctt
     1441 cttatagaaa tccttgtatt cgtcatcata acgccttacc gagttggcgg cttcaaccag
     1501 gtagtagcgc agataacgat tgcccctatt cacaagcgaa gtatgttgtg agtttgagtt
     1561 accagattgc ttttctctcc aattcagacc agcaaatttt gcgacctggg gatggtcttt
     1621 aaagcgttcg atttgtccaa tttcggcaat gataccagca gcgtagacct tcccgacccc
     1681 aggtatggag gtcaggcatt gatattcagg aatggtttcc accatatctt caatggcctt
     1741 atcaatttcc ttgatcattt tttccaggtt tcgaatttcc cgagccagaa gtccgagaat
     1801 gacattgact gaatcttgtt ggagtttaga gaggcgataa gaacctctga tagcggcttg
     1861 aatggtttga gcaagcttct cagagtcctt aaaatgccct ttacccagtt tttggagcca
     1921 atcatctaat tcttgaagag gcatgttagc aagttcgtct aaggtcatct catctgtcat
     1981 caaggtcatc atcgttgaac tccataggtt agtagagagt tcttctttct tgaattgata
     2041 agccaaggta ttgcacttgt agtagatatt ttcaatgaag tgctgtttgg ttctggttaa
     2101 ttgctcgatg atttggagtc tggttcttgt gagatgttgg agagccatgt atttctcttc
     2161 ttttaaaaaa gaaggtgaaa atcgctcaat acggaaataa tccgcaatgt agaaagcatc
     2221 aatggtatcg tttttgtttt cttcgaaagc atcccgatac ttcttgattt tattaggttg
     2281 ttcgaccata atctcaacat gtagttcttt caactgccta tcttcatgga agaacatagc
     2341 agggtggaaa ctgtagagac tggttgcttc catgccaatg acaatccttt cgaagtgata
     2401 ggtatgattc aaatcaagga tatgattctt aatctctgtt gccccagaaa ggtcattaga
     2461 gagggaagca gtgacggggg tgctagtatc actcaacatg atacacacat ctaattttgt
     2521 agagctgatg tctaaaccga caaaacattt cattaaggaa ggtctccttt ccattagatt
     2581 tggaagtttt tttgactact gtaaggtttc ccagaaacac cttgttccaa catcctcgca
     2641 taagagaatc cttatcacag cctgacagag ccgcctgcat cctactagat acagaagtca
     2701 agctgtggaa acagctagtg tgttggaagt ttgaacaagg cttgggtaac agacttttta
     2761 atgaagtcca agcaacaagg agaaaaagaa acaaccatga gtccattcca tgactctatt
     2821 gttctagaaa accttgcaat agtcaagtga taacgaataa aacttgggat tgaaatatcc
     2881 ccatgattat atattacgag gagaaactat gtttaaagaa tggaaagcaa tttaaaaaaa
     2941 acaaccttta ttattgtcat gatcgggatt tctcttattc cagctctgta caatatcata
     3001 tttttgtcat caatgtggga tccatatggg caattgtctg acttacctgt ggcagttgta
     3061 aataatgata aagaggcttc ctataatggt aatactatgg caataggaaa agacatggtg
     3121 tccaatttaa aagaaaataa aaccttggat tttcattttg tagatgaaga ggaaggaaag
     3181 aagggattgg aagatggcga ttactatatg gtagtgactt taccaagtga tttatctgaa
     3241 aaagcaacta cattatccaa tattcaatcg acagcagctt atcaatcatt gacaagtgag
     3301 caacaaactg agataagtga ttctgtatct caaaattcaa ctgatagtat tcaatcggct
     3361 cagtcaattg tagctttagt acaagattta cagggaagtt tagaaaactt acaaaatcaa
     3421 tcttctaatc tttcgacttt aaaaaatcaa gctaatcaag tattacctat tacttctact
     3481 tctttgatag gattgtcaag tggattaaca gagatacaag gagctgttac tagcaaatta
     3541 gttcctgcca gtcagtcgat tgcatcaggt gtaaacgcat atactacagg tgttgataaa
     3601 gtttctcagg gcgcaagtca actaagtgaa aaaaatgcta ccttgacagg tagtttggat
     3661 caattagttt caggctcaaa caccttgaca caaaaatctt ctagcttgac agcaggagtt
     3721 ggtcaattag ttgaaaaaac tccagaatta gtatctggta ttgaaaaatt atcaactggc
     3781 tccaattaat tgaatcaaaa gagtcaagaa ttgatagcag gagttgataa attgcagtca
     3841 ggctctagcc aactagctga caaatccagt cagttacttt caggtgtttc tcaattagaa
     3901 agtggagcta ataaattggc agatggagct gggaaactag cagaaggtgg aacaaagtta
     3961 acttctggat tggaaggttt acagacagga cttgcttctt taggacaagg actaggtaat
     4021 gctagtgatc aactcaaatc agcatcaaca gaatctaaaa atgcagagat tttgtcaaat
     4081 ccactcaatc tttccaaaac agacaatgac caagttcctg taaatggaat tgcaatggct
     4141 ccttatatga tatcagttgc tctttttgtt gcagcaatat caacgaatat gatctttgcg
     4201 aagttacctt caggacgtca tccagagagc cgttgggctt ggttgaaatc tcgagctgaa
     4261 ataaatggta ttatagctgt tttggcaggg attttggtat atggaggagt tcatcttatt
     4321 ggtctaactg ctaatcatga aatgagaaca tttattctca tcatcttaac aagtttagta
     4381 ttcatgtcta tggtgaccgc tttaacaaca tggaatagcc gtataggagc tttcttctcg
     4441 cttatttttc ttttattaca gttagcatca agtgcaggta cctatccgct tgctttgaca
     4501 aatgatttct ttagagctat taatccttgg ttgccaatga gttattcagt ttcgggatta
     4561 cgacaaacaa tctccatcaa caagtcattt tcctagctgt catactagct ctatttactg
     4621 gtttaggtat gctagcctat caacctaaga aaatggaaga agattaaaaa aatcgaccga
     4681 ttaactggtc gattttttat gccttagatg actttcgtct gtgattatag attccaaata
     4741 gtaagagaga agtaaaggaa cagattgctc cagtaataaa accattggga atgaaggaaa
     4801 gtgtaatagt tcctttcccc ttgggaatgt caactttcat aaatccagtt tgagcttgtt
     4861 taatttctat tttcttacca tcttggtagg cagaccaacc tttgtcataa ggaatggtga
     4921 agaaaataga tgtatcttgt tggacatcat atgtagcaaa aaccttgttt ttagaagttg
     4981 atactgtgac aggttgttct ttaatttttt gaattgcctc ggtgaaagtt ttggtatcta
     5041 aacgatagaa ggtaggagat tcaaatgata cttgtgaatt tccagggaaa ctaacattga
     5101 tattgaaagt ttttttctct ttagtatatc ctagattaaa gaaggagaag acattatcag
     5161 ttgtaaaagt ctttttttca ccatttacaa ggatgtcaac cttcttttgt ttatcgttag
     5221 aaaagtgaag gtttatgaaa gagagataaa tttggctgtt ttctggaact tcaatttgat
     5281 actggattgc tgcatcctca tttgaagaac ttgtgacact aatcaaatta ttagtatttt
     5341 ctattttttc ataagggatt ggagaaaaat aatcaaaatc aacgttagca agttgattta
     5401 aaaacgaggc ctgattatcc aaggtatgtt cattgaactt gacatcattg taaacagatt
     5461 gactagcaac tgcaatcgga agagagtatt gattttcata tagggtaaga ttatcttttt
     5521 gatagatatc tttaaaacca tacttatcaa taggactgtc tgagatattg tactggatac
     5581 caaataaact atcagccaaa atactattat ttgcatatcg gagattgaga ttagtcccag
     5641 aggatttaaa cccaagttta tctaaagtag agcttgatga acgatttcga acagatgaaa
     5701 attgagagat tccattgtag ttgaatttca tactgtcatt tcctgtctga gtttgtagtt
     5761 tttcagtacg agtaaattga tttccaatat atgttgagaa agattccata gctgggatat
     5821 ctcgactata agtacttcga gaagcaaatc cccattcctt agcaattccg tccatttgag
     5881 atgaagcatt taaactcatt tcaaccagta taaataaaga gattagaatg gcaaatagat
     5941 tcacagatat aaactttttg ataactgcaa ggagtaaaag agaatagaca accaaaaatt
     6001 caagagtaag cagaatattc aaatctgtta aaaaagaata atgcgatttt agatagatgg
     6061 tagctaaaaa tcctgttact acaagaaaaa gcgaaactaa aaaattccag actttaagtt
     6121 ctttcagacg ctttaagact tctgctgctg tgtaaattaa caaggtagag aaaatccaag
     6181 catagcgatg taaaaacatg tttggagtat gcatgccttg ccaaaataag tcaagagctt
     6241 ctatgtaaaa gcttgcaatt agaaatgcaa agaataatac atatatgagt ttcacgtgaa
     6301 acttaataga tttcagcgta aaaaataaaa tggtcaaaat aaaaggaagt agtccaacaa
     6361 aaatcattgg gatggcccca tactttgttg tgtcaaagga accaatgaat tgcttagcaa
     6421 agagatcaag ataccagcta ctttcagttt gaaactttgt aacttcagtc aatttttccc
     6481 catgtgtctg taaatcaaac agagtgggaa gagtcataat caaactagcc ataccagcta
     6541 aaaaggagat aactatgaaa tcaagaacag atgattttcg agtcttaaag tcccacgaaa
     6601 tttgacagag ataccagaaa ataagaaaca atactgtcat atatccaaaa taataatttt
     6661 gaataaataa gattgacaga cttgtaaagt acaataggag tttcttttca gttatcagta
     6721 gatgtaaacc agttataatt aaaggaatca agataaaaac atctagccag gtttttatct
     6781 ctaattgact gacagtgaaa ctcatcagag tataggaagt agataaggct agttttaaag
     6841 tctgagggat agattgaaac aatttattca aactaaaaaa ggttgacaga ccaatcaatc
     6901 caaattttaa gagagtggtc agataaacag catctggcat attcgttaga tcaaaaaagt
     6961 aaaccagagg tgagagaaaa ctacccaagt aataactaga cagagcatag aagtttagcc
     7021 ctagaccact tgtaaaggtg taaaacagac tactatttcc atgtaggata tttcgtaagg
     7081 ctacatcaaa aataacgtat tgatgaaagc catctcctaa tagaggagag ttgtcgctat
     7141 tccagtagat accttgagat agatatactc cagacataat cactacagga atgatgagag
     7201 aaataaaata ggttcgatat gtttttaaaa atgatttcat gttacctcgt agaatgatag
     7261 aaaactcagt tggttaaccc aactgagttt tgaagtttta tttagtcttt ccaaagttct
     7321 ttaacttttg cttgtacttc tgcattttct aggaattcat cgtaggtttc atcgatacgg
     7381 tcaatgacgc catttttaga taagacaatg atatggttag ccaaagtttg aataaattcg
     7441 tggtcatggc tagcaaagat gattgattct ttaaagtttt tcaatccatc attcaagctt
     7501 gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa gtacaaggac atttgatttt
     7561 aagagcatga gttttgaaag catgacacga actttttctc cccctgacaa gacatttaca
     7621 ggtttgttaa cttcatctcc agagaagagc atacggccga ggaagccacg taggaaagta
     7681 ttgtcatctt cttctttact tgcgaattga cgcaaccagt caagaattga ttctcctcct
     7741 gcaaaatcag ctgagttatc ttttggtagg taagattgac tagttgtaac tccccacttg
     7801 acagttcctt catagtcaat atctcccatg attgcacgaa ttaatgcagt cgtttgaata
     7861 tcattttgtc caataagtgc tgtcttatca tctggacgca agatgaaact aatattatcc
     7921 aagatagttt caccatcaat ctttacagtt agattttcta ctgtcaagag atcattacca
     7981 atctcacgtt ccgctttaaa gttgataaat ggatatttac gactagatgg tacaatctct
     8041 tctagctcaa tcttatcaag cattttctta cgtgatgttg cctgccttga cttagaagca
     8101 ttggcagaga aacgagcaac gaattcttgc aattgtttaa ttttttcttc tgctttagca
     8161 ttacggtctg ctagcaattt agcagcaagc tcagaagatt ccttccagaa gtcgtagttt
     8221 ccgacataga gtttgatttt tccaaagtca aggtcggcca tgtgagtaca aactttgttt
     8281 aagaagtgac ggtcgtggga tactacgata actgtgttat caaagtcaat caagaagtct
     8341 tctaaccaag taatcgattg gatatccaaa ccgttagtag gctcgtccaa gagaagaaca
     8401 tctggtttac caaaaagtgc tttggcgagg agaaccttta ctttttcacc gttggccaat
     8461 tcgctcatgt tttggtagtg taattcttct ggaatgttta ggttttgaag tagttgagag
     8521 gcttcactct ctgcttccca acctccaagt tcggcaaact ctccttcgag ttcggcagca
     8581 cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga tagcatcttt ctctttcatg
     8641 atgctataaa gtttttcatt tcccatgata acgacatcaa tggcacgttc atcttcgtag
     8701 tcaaagtgat tttgacgaag aacagagaga cgttcatctg gaccaagaga gatgtgacca
     8761 gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg ttgattttcc ggcaccatta
     8821 gcaccgatta atccgtaagt atttccttct gtaaatttga tattgacatc atcaaaaagt
     8881 ttgcgatcac taaaacgtag tgaaacatca gatactgtaa gcaatatttt tctcctatat
     8941 gtgtaatata tttattctac tagaaaatac agaaatattc aaatttttat ttgtcaattt
     9001 tgtgtaaatt atatttacag tatcctttac acaaatctgt aaaaagcaag gctgatttat
     9061 tttgataaat tacggttatt tcattaaaaa aatgctataa ttgaaaggac tatatcgaag
     9121 gagaacaaaa tgactaaacc cattatttta acaggagacc gtccaacagg aaaattgcat
     9181 attggacatt atgttggaag tctcaaaaat cgagtattat tacaggaaga ggataagtat
     9241 gatatgtttg tgttcttggc tgaccaacaa gccttgacag atcatgccaa agatcctcaa
     9301 accattgtag agtctatcgg aaatgtggct ttggattatc ttgcagttgg attggatcca
     9361 aataagtcaa ctatttttat tcaaagccag attccagagt tggctgagtt gtctatgtat
     9421 tatatgaatc tagtttcgtt agcacgtttg gagcgaaatc caacagtcaa gacagagatt
     9481 tctcagaaag gatttggaga aagcattccg acaggattct tggtctatcc aatcgctcaa
     9541 gcagctgata tcacagcttt caaggctaat tatgttcctg ttgggacaga tcagaaacca
     9601 atgattgagc aaactcgtga aattgttcgt tcttttaaca atgcatataa ctgtgatgtc
     9661 ttggtagagc cggaaggtat ttatccagaa aatgagagag cagggcgttt gcctggttta
     9721 gatggaaatg ctaaaatgtc taaatcacta aataatggta tttatttagc tgatgatgcg
     9781 gatactttgc gtaaaaaagt aatgagtatg tatacagatc cagatcatat ccgcgttgag
     9841 gatccaggta agattgaggg aaatatggtt ttccattatc tagatgtttt tggtcgtcca
     9901 gaagatgctc aagaaattgc tgatatgaaa gaacgttatc aacgaggtgg tcttggtgat
     9961 gtgaagacca agcgttatct acttgaaata ttagaacgtg aactgggtcc tattcgtgag
    10021 cgccgtattg aatttgctaa ggatatggga gaagtttata atatgattca aaaaggtagt
    10081 gaaagagcgc gtgaagttgc gggtcaaacc ctatctgagg taaaaggggc aatgggactt
    10141 cattacttta actaagttta ttttacaaga aactatcatt tctatctcga agaaaagata
    10201 gttttttatt tacccctgta acatttgaca aatttttata gaatggtatg atagatacaa
    10261 tataaaaaga gtcaagctca aaaagaaaga aaagaggaaa cttcgaatgt ctaattggga
    10321 cactaaattt ttgaaaaaag gttttacctt tgatgatgta ttgcttattc cagctgaaag
    10381 tcatgtgttg cctaacgatg cagatttaac aactaaattg gcagataatt tgactttaaa
    10441 tatcccaatt attaccgctg ccatggacac agttacagag agtcaaatgg ccattgctat
    10501 tgctcgtgca ggtggtctcg gagttatcca taaaaacatg tcaattgctc aacaagcaga
    10561 cgaggttcgt aaggtaaaac gttctgaaaa tggagttatt attgatccgt tcttcttgac
    10621 gcctgaacat acaattgctg aagcagatga gcttatgggt cgttaccgca tcagtggtgt
    10681 tccagttgtt gaaacacttg aaaatcgtaa attggttggt attttgacaa accgagatct
    10741 tcgttttatt tcagattata atcaaccaat ttcaaaccat atgactagtg aaaatcttgt
    10801 tactgctcct gtgggtacgg atcttgcaac ggctgagagt attcttcaag agcatcgtat
    10861 tgaaaaactt ccgttggtcg atgaagaagg cagtctttct ggtttgatca ctatcaaaga
    10921 tattgaaaaa gttattgagt ttccaaatgc ggctaaagat gaatttggtc gtcttctagt
    10981 tgcaggtgca gtaggtgtta cttcagatac atttgaacgt gcagaggctc tttttgaggc
    11041 aggagcggat gcgattgtta ttgatactgc acatggtcat tctgcaggtg tcttgcgtaa
    11101 aattgccgag attcgtgctc atttcccaga tcggactttg attgctggaa atattgctac
    11161 tgctgaaggt gcacgtgccc tttatgaagc gggtgtagac gttgttaagg ttggtattgg
    11221 accaggttct atctgtacta ctcgtgtgat tgctggtgtt ggtgttccgc aagtaacagc
    11281 tatctacgat gctgcagctg ttgcgcgcga atatggtaaa acgattattg ctgacggtgg
    11341 gatcaagtat tctggagata ttgtaaaagc acttgctgca ggtggaaatg ctgttatgct
    11401 tggatctatg tttgctggaa ctgatgaagc tccaggcgaa actgaaatct tccaaggacg
    11461 taaattcaag acttaccgtg gtatgggatc aattgctgct atgaagaaag gttcaagcga
    11521 ccgttatttc caaggttctg tcaatgaagc aaacaagctc gttccagaag gaattgaagg
    11581 tcgtgttgct tataaaggag cggcagctga tattgttttc caaatgattg gtggtattcg
    11641 ctctggtatg ggttactgtg gtgcagctaa ccttaaagaa ctacacgata atgctcaatt
    11701 tattgaaatg tctggtgctg gtttgaaaga aagccatcct catgatgtgc aaattactaa
    11761 tgaggcacca aattattcta tgtaaaaaac aatgaaaaga actccagtga aaacaggagt
    11821 tcttttacaa tgttgtcaat ttccatttac agaagcttta ccatcctgaa tagtgaagat
    11881 acttagattt tctggcagat tttgaagatg gtctaagctt gttgttgtga taaaggtttg
    11941 gattgattga gaaatcgttt ctaataattt taactgtcta gtgttgtcaa gttcactcat
    12001 cacatcgtca agcagtaata taggagattc tgtggtaatg ctttccatta attcgatttc
    12061 tgctaatttt atcgagagga cgagactacg atgttgacct tggcttccga aactagcatc
    12121 catcccattt atataaaaag aaatgtcatc tcgatgagga ccgacaccag tattcttttt
    12181 aaataaatct ctggatctac ttttttctaa agcaattttg aaagattcgg ataagttttg
    12241 tttgtcagtt atattgacag aagattgata ggatattgac aactcttcga tctgattaga
    12301 gagttcaaaa tgtttcttac gcccaaatga ttctagtttt tttatgaaat ctaagcggtg
    12361 attcattaca cgacatccat aatcaactag ctgatcatct aacacagaaa ggaatgtttc
    12421 atctattttt tgagctgatt ttaggtaagt gtttctttgc tttaggatgt ggttataatt
    12481 ggttaagtca gataaataga ttggcttaat ttgcccaagt tccatatcaa tgaattttcg
    12541 tcgaatcgaa ggtgctcctt taattagttg taaatcttca ggagcaaata agacaacatt
    12601 catgtgtcct acataatctg aaaggcgtgc ctgttttaag tgattaactt ttgttacacg
    12661 gcctttttgt gttagttcga tttctagggg aatggatcca gtttttttct gaacgagacc
    12721 tgaaagatga agttgttcct catcaaaatg aatgagattt ttatctgttc gagttcgatg
    12781 actacgcgtt aaggctaaaa aatagatagc ctctaacatg tttgtttttc cttgtgcatt
    12841 acgtcccaaa aagacattca atttaggatt aaagtctatt ttcgtttctt tgtaattacg
    12901 aaaagtcttg agagatagat gttgtagcca catgattatc taccagggaa tcgtggagct
    12961 tgttggcttt taggtgatga agcaggtttc gatttgtctt ttttaactcc cttattcatc
    13021 tttttgacaa gtttagcgat ccgttctttt tcaactttat cagcttggta ttcatcttgt
    13081 tcttcagaag taggttgtgt caacaagatg tcaatgttca tgtcagggat gtcaacttta
    13141 tcgccaatac gaagtttttt tccacgacga ctctctaatt ccctattaaa gtaaacagaa
    13201 tgttcagaga gaaatgattt aatagctcct ccgctatgtg taattccaag ttctttgagt
    13261 agtgcttgga gggtaataaa ttcttcaaat aatttgtatt ccataattca cctcaatctt
    13321 tggtattata ccatattttc ctaaaaatag tgagagagac agcgagaaat gtaagcgatt
    13381 tttatttcaa aagtgttaca gggaacaaga aaatttcagg ttttcgtgat ataatagaag
    13441 tctgtatata aggaggtaaa tcatggagtt agtgcatgga atttcaacac attttatcca
    13501 atcaaaaaag tttaaaacaa acaaaattac cgtgcgtttt accgctccat tatcccttga
    13561 tacgattgca ggtcacatgt tgagtgcaag tatgctagag actgctaatc agatgtaccc
    13621 cacttctcaa gatttgagga gacacttggc cagtctatac ggtacagata tgtcaaccaa
    13681 ttgtttcaga agagggcaaa gccacattat agaattgaca tttacctatg ttcgtgatga
    13741 gtttttaagt aggaaaaacg tgctaacctc tcagattttg gaacttgtaa aagaaactct
    13801 tttttcaccc gcagtagttg ataatgggtt tgatccggcc ttatttgaaa ttgagaaaaa
    13861 acaattgcta gcaagtttag cagctgatat ggatgattct ttttattttg cacataaaga
    13921 attggataaa ttgttttttc atgatgaacg tcttcaattg gaatatagtg atttacgaaa
    13981 tcgtatttta gctgaaactc cacaaagttc ttattcttgt ttccaagaat ttttagccaa
    14041 tgatcgaata gatttctttt tcctaggtga ttttaatgag gttgaaattc aaaatgtatt
    14101 agaatcattt ggctttaaag gtcgaaaagg agatgtgaag gttcagtatt gtcaacctta
    14161 ttctaatatc cttcaggaag gtatggttcg gaaaaatgtg ggacaatcca ttttggaatt
    14221 aggttatcat taccgttcta aatatggtga tgagcaacat ttacccatga ttgtaatgaa
    14281 tggtttactt ggtggatttg ctcactctaa gctctttaca aatgtccgtg aaaatgctgg
    14341 attagcttat accatttcaa gtgagcttga tttatttagt ggattcttga ggatgtatgc
    14401 tggtatcaat cgagaaaatc gtaaccaggc tcgtaaaatg atgaataatc aactgcttga
    14461 tttaaaaaaa ggttatttta cagagtttga gttaaatcag accaaggaaa tgattcgttg
    14521 gtcgttgtta ctttctcaag ataatcaatc ttcattgatt gaacgtgctt atcaaaatgc
    14581 cttatttgga aaatcttcag cagactttaa aagttggatt gcaaagcttg aacaaattga
    14641 caaagatgct atttgtagag tagctaataa tgtgaaacta caagcgattt actttatgga
    14701 aggaatagaa tgacaaaggt tgtttttgaa gaaaaatact atccagctgt aaaagaaaag
    14761 gtttatcgaa ctcgtttggc caacggattg acagttgctc ttttgcctaa aaaggaattt
    14821 aaagaggttt acgggagtgt cactgtacag tttggttcgg tagatacgtt tgtcacagaa
    14881 gttgacggat atgtaaaaca atatcctgga ggaattgctc attttcttga acataaatta
    14941 tttgagagag aagattctag tgatttgatg tcggctttta cgagtctagg tgcagatagt
    15001 aatgccttta caagctttac aaaaacaaac tatctttttt cagcaacgga ttatttttta
    15061 gaaaatttag atttacttga tgaattggta acatcagcac actttactga agcttccatt
    15121 ctgacagagc aggatattat tcagcaagaa cgagaaatgt accaagatga tccagattcg
    15181 tgtttattct tttcaacttt agcgaatttg tatcctggta cacctttagc aactgatata
    15241 gttggaagtg aggagtccat ttcccaaatc aatctaacta atttgcaaga aaattttaca
    15301 aagttttaca aacctgtaaa catgtctctg tttttagttg gtaattttga tgtggagcga
    15361 gtacaggact attttgaaag caaagaactg aaagattcag attttcagga agtagcaaga
    15421 gaaaagttgt ttttacagcc tgtaaagcca acagatagta tgagaatgga agtatcttct
    15481 cccaaactag cgattggagt tagaggtaag cgagaagttt ctgaagcgga ttgctatcga
    15541 catcatattt tattaaaatt attgtttgca atgatgtttg gttggacttc ggatcgtttt
    15601 caaaaatgtt atgaatcagg taaaattgat gcgtccttat ctctggaagt tgaaataaca
    15661 agtcgctttc attttgtcat gttgacaata gatacgaaag agccagttgc tttgtctcat
    15721 caatttagga aggctattcg taattttaca aaggatttag atattacaga ggaacattta
    15781 gatattatca aaagagagat gtttggcgaa tttttcagta gcatgaactc tcttgaattt
    15841 attgcaacgc aatatgatgc ttttgaaaat ggtgagacaa tttttgattt gccgaaaatt
    15901 ttacaggaaa ttactttaga ggatgtcctt gatgctggac atcatttaat agatgatggt
    15961 gacatagttg attttacaat attcccatcg tagtaaccta tcataataga cactagaaag
    16021 aagggatgac aagtatgaga aaaaaaacaa ttggagaggt tttacgatta gctagaatca
    16081 atcagggatt gagtttagat gaattgcaga aaaagacaga aatccagtta gatatgttgg
    16141 aagcaatgga agcagacgat ttcgatcaac ttccaagtcc tttttacacg cgttctttct
    16201 tgaaaaaata tgcatgggct gttgagttag atgaccaaat tgttttggat gcttatgatt
    16261 ctgggagtat gattacttat gaggaagtag atgttgatga agatgagttg acaggtcgta
    16321 gacgttcaag taagaaaaag aagaaaaaaa catcattttt acctttattt tattttatcc
    16381 tttttgcttt atcgatttta atttttgtga cttattatgt ttggaactat attcaaactc
    16441 aaccagagga gccttctctt tctaattaca gtgtggttca atcaacaagt tcaactagct
    16501 ctgttcccca ctcctcaagt agtagttctt ctagtataga atcagctata agtgtatcag
    16561 gcgaaggaaa tcatgtagaa atcgcttata agacaagtaa ggaaacagtt aaattgcaat
    16621 tggcagtttc agatgttaca agttgggtca gtgtttcaga aagcgaactt gagggcggtg
    16681 taaccttatc gccaaagaag aaaagtgcag aagcaacagt tgcaactaaa agtcctgtaa
    16741 caattacgtt aggtgttgta aaaggtgttg atttgacagt agataatcag actgttgatt
    16801 tatcgaaatt aacagctcag actggacaaa tcactgtaac ctttactaaa aattaaggaa
    16861 aaacgaatga aaaaagaaca aattcccaat ctcttaacaa taggtcgaat tctctttata
    16921 cctattttta tctttatttt gacgatagga aattcgatag agagtcatat agttgcagct
    16981 attatctttg ctgttgccag tattaccgac tatttagatg gatatttagc tcgtaaatgg
    17041 aatgtggtca gtaattttgg taaatttgca gatcctatgg cggataagtt actagttatg
    17101 tcggctttta ttatgttgat tgagttaggt atggctccgg cttggattgt tgcagtgatt
    17161 atctgtcgtg agttagctgt gacaggttta aggcttttat tggttgaaac tggtggaaca
    17221 attttagcag cagcaatgcc tggaaaaatt aaaactttta gtcagatgtt tgctattatt
    17281 ttcttgctat tacattggac tttgcttggt caagttctac tttatgtagc cttatttttc
    17341 actatctact ctggctatga ctatttcaag ggtagtgcct atgtatttaa agggacattt
    17401 ggttcgaaat gaaatcaata attgatgtaa aaaatctttc ttttcgctat aaagaaaatc
    17461 agaactacta cgatgtgaag gatattacgt ttcacgtgaa acgtggagaa tggctttcga
    17521 ttgtagggca taatggtagt ggtaaatcaa cgacggttcg attaattgat ggcttactgg
    17581 aagcagaatc cggagagatt gtaattgatg gccaacgttt gactgaggaa aatgtttgga
    17641 atatacgtcg tcaaatcggt atggtttttc aaaatccaga caatcaattt gttggagcga
    17701 ctgttgaaga tgatgttgct tttggtttgg aaaatcaggg actttctcgt caagaaatga
    17761 aaaagagagt ggaagaagct ctggctttag ttggcatgtt ggactttaaa aagagagagc
    17821 cagcgcgtct atcaggtggc caaaagcaac gtgtggccat tgcaggtgtt gtagccctaa
    17881 gaccagctat tttaatctta gatgaagcaa cgagtatgtt ggatcctgag gggcgtagag
    17941 aacttattgg gacagtaaaa ggaattcgaa aagactatga tatgacggtc atttctatta
    18001 cccatgattt ggaagaagtc gccatgagtg atcgcgtatt ggtcatgaaa aaaggggaaa
    18061 ttgaatcaac tagtagtcca agggagcttt tctctcgaaa tgatttagat caaattggat
    18121 tagacgatcc ttttgctaat caattaaaaa aatctttgag ccagaatggc tatgatttac
    18181 ctgaaaatta tttgacagaa agtgagctag aggataagct atgggaattg ctctagaaaa
    18241 tgtgaatttt atatatcaag aaggtactcc cttagcttca gcagctttgt cggatgtttc
    18301 tttgacgatt gaagatggct cttatacagc tttaattggg cacacaggta gtggtaaatc
    18361 aactatttta caactcttaa atggtttatt ggtgccaagt caagggagtg tgagggtttt
    18421 tgatacctta atcacctcga cttctaaaaa taaagatatt cgtcaaatta gaaaacaggt
    18481 tggcttggta tttcagtttg ctgaaaatca gatttttgaa gaaacggttt tgaaggacgt
    18541 tgcttttgga ccgcaaaatt ttggagtttc tgaagaagat gctgtgaaga ctgcgcgtga
    18601 gaaactggct ctggttggaa ttgatgaatc actttttgat cgtagtccgt ttgagctgtc
    18661 agggggacaa atgagacgtg ttgccattgc aggcatactt gccatggagc catctatatt
    18721 agtcttagat gagccaacag ctggtctaga tcctctaggg agaaaagagt tgatgaccct
    18781 gttcaaaaaa ctccaccagt cagggatgac catcgtcttg gtaacgcatt tgatggatga
    18841 tgttgctgaa tatgctaatc aagtctatgt aatggaaaag ggacgtttag taaagggggg
    18901 caaaccaagt gatgtctttc aagacgttgt ttttatggaa gaagttcagt tgggagtacc
    18961 taaaattacg gccttttgta aacgattggc tgatagaggc gtgtcattta aacgattacc
    19021 gattaagata gaggagttca aggagtcgct aaatggatag tatgattttg gggcgttata
    19081 tcccagggga ttcgattgtt caccgattgg atccacgtag caaattgctt gctatgatgc
    19141 tactgatttt gattgtgttt tgggctaata accccttgac gaatctgatt ctttttatag
    19201 cgacagggat atttattgcc ttgtcaggag tatctctttc attttttatt cagggcttga
    19261 agtctatgtt tttcttgatt gccttcacaa ctatttttca actatttttc atttctaatg
    19321 ggaatgtttt atttgagttt tcgtttgtga gaatcacgga ttatgctttg caacaagctg
    19381 ggattatttt ttgtcgcttt gtattgatta ttttcttttc aactttgtta accttaacaa
    19441 ccatgccttt aagtttggca tcagctgtcg aagctttatt agcaccttta aagcgtgtga
    19501 aagttccagt tcatgaaatt ggattgatgc tgtctatgag tttgcgtttt gtcccaacct
    19561 tgatggatga tacgactcgg attatgaatg cacagaaagc tcgtggagtg gattttggag
    19621 aaggaagcat cgttcaaaaa gtaaaggcga tgattcccat tttgattcct ctttttgcga
    19681 caagtttaaa acgtgcagat tccttggcta tcgctatgga agcgcgtggc tatcagggtg
    19741 gaaaaggcag aagtcaatac cgacaattga aatggactct aaaggatacg ctgaccattc
    19801 ttgttattct cgtacttggt tgttgtttat ttttcttaaa atcttagtag ctttcaggaa
    19861 ttgataaaaa gttactgtaa cagtttttga tataaaggta gggatatgaa ccgttttaaa
    19921 aaatcaaaat atgtcattat tgtttttgtc actgttctgc ttgtgtcagc tctcttagcg
    19981 acgacttatt caagtacaat tgtgacaaaa ttaggagatg gaatctcatt ggttgataga
    20041 gttgtacaaa aaccttttca gtggtttgat tctgtcaaat cagatttggc tcatttgaca
    20101 cgaacatata atgaaaatga aagtttgaag aaacagcttt accaattaga agttaaatca
    20161 aatgaggtgg aaagtttaaa gacagaaaat gaacaactgc gccaattgct tgatatgaag
    20221 tctaaattgc aagccacaaa gactttagca gcagatgtta ttatgcgttc tccggtatct
    20281 tggaagcagg agttgacctt agatgcaggt agatcaaaag gtgcttctga gaacatgtta
    20341 gctattgcaa atggtggctt gattgggagt gtttcaaaag tagaggagaa ctctactata
    20401 gtcaaccttc tgacaaatac ggaaaatgct gataagattt ctgttaaaat tcaacatggc
    20461 tctactacaa tttatggaat tattattggc tatgacaagg aaaatgacgt tcttaaaatt
    20521 agccaattaa atagtaatag cgatattagt gcgggagata aggtgactac tggtggatta
    20581 ggaaacttta acgttgctga tattcctgtt ggtgaagtgg ttgccacaac gcatagtaca
    20641 gactatttga cacgagaagt aactgttaaa ttgagtgcag atactcataa tgtagatgtg
    20701 atagaattag tggggaattc ataatgagac agttgaagcg agttggagta tttttattgc
    20761 ttcctttctt tgttctaatt gacgcccata ttagccagct tctgggctca tttttccccc
    20821 atgtacattt ggctagtcat tttctttttc tatttctctt atttgagacg atagaagtat
    20881 cagagtatct ctacctagtc tattgttttg ttataggctt ggtttatgat gtttactttt
    20941 tccatctaat agggattaca actctcttat ttatcttatt gggagccttc cttcataaat
    21001 tgaatagtgt tattttgttg aatcgttgga caagaatgct agctatgatt gtgctgacat
    21061 tcctgtttga aatgggtagt tatcttttgg cttttatggt agggttgaca gtagatagca
    21121 tgtcgatttt tatagtctat agcttggtac cgacgatgat tttaaatttt ttatggatta
    21181 ctgtttttca atttattttt gaaaaatatt atctataaga acgacatata aatgtaacaa
    21241 aggcgtaata tttattaggc ctttttttgg tatactagta ttgtctttaa aagaaggagt
    21301 atctacgtaa tatgaagaaa aaaatcttag cgtcactttt attaagtaca gtaatggttt
    21361 ctcaagtagc tgttttaaca actgcgcatg cagaaacgac tgatgacaaa attgctgctc
    21421 aagataataa aattagtaac ttaacagcac aacaacaaga agcccaaaaa caagttgacc
    21481 aaattcagga gcaagtatca gctattcaag ctgagcagtc taacttgcaa gctgaaaatg
    21541 atagattaca agcagaatct aagaaactcg agggtgagat tacagaactt tctaaaaaca
    21601 ttgtttctcg taaccaatcg ttggaaaaac aagctcgtag tgctcaaaca aatggagccg
    21661 taactagcta tatcaatacc attgtaaact caaaatcaat tacagaagct atttcacgtg
    21721 ttgctgcaat gagtgaaatc gtatctgcaa acaacaaaat gttagaacaa caaaaggcag
    21781 ataaaaaagc tatttctgaa aaacaagtag caaataatga tgctatcaat actgtaattg
    21841 ctaatcaaca aaaattggct gatgatgctc aagcattgac tacgaaacag gcagaactaa
    21901 aagctgctga attaagtctt gctgctgaga aagcgacagc tgaaggggaa aaagcaagtc
    21961 tattagagca aaaagcagca gctgaggcag aggctcgtgc agctgcggta gcagaagcag
    22021 cttataaaga aaaacgagct agccaacaac aatcagtact tgcttcagca aacactaact
    22081 taacagctca agtgcaagca gtatctgaat ctgcagcagc acctgtccgt gcaaaagttc
    22141 gtccaacata cagtacaaac gcttcaagtt atccaattgg agaatgtaca tggggagtaa
    22201 aaacattggc accttgggct ggagactact ggggtaatgg agcacagtgg gctacaagtg
    22261 cagcagcagc aggtttccgt acaggttcaa cacctcaagt tggagcaatt gcatgttgga
    22321 atgatggtgg atatggtcac gtagcggttg ttacagctgt tgaatcaaca acacgtatcc
    22381 aagtatcaga atcaaattat gcaggtaatc gtacaattgg aaatcaccgt ggatggttca
    22441 atccaacaac aacttctgaa ggttttgtta catatattta tgcagattaa tttacagagg
    22501 gactcgaata gagccctctt ttcaggtttt accgtgacaa tccctattaa aaattatatc
    22561 aaaatagctt gaaaatattg gaaaagtatg gtaaaatgaa aattgtcgtg tgaacgataa
    22621 tactcattct tgatgaattg tgaagcagtt gcccttgggt cgttttgcga gttgaagtca
    22681 agaagaggaa aaaaacaaaa aggagaaata ctcatggcag taatttcaat gaaacaactt
    22741 cttgaggctg gtgtacactt tggtcaccaa actcgtcgct ggaatcctaa gatggctaag
    22801 tacatcttta ctgaacgtaa cggaatccac gttatcgact tgcaacaaac tgtaaaatac
    22861 gctgaccaag catacgactt catgcgtgat gcagcagcta acgatgcagt tgtattgttc
    22921 gttggtacta agaaacaagc agctgatgca gttgctgaag aagcagtacg ttcaggtcaa
    22981 tacttcatca accaccgttg gttgggtgga actcttacaa actggggaac aatccaaaaa
    23041 cgtatcgctc gtttgaaaga aattaaacgt atggaagaag atggaacttt cgaagttctt
    23101 cctaagaaag aagttgcact tcttaacaaa caacgtgcgc gtcttgaaaa attcttgggt
    23161 ggtatcgaag atatgcctcg tatcccagat gtgatgtacg tagttgaccc acataaagag
    23221 caaatcgctg ttaaagaagc taaaaaattg ggaatcccag ttgtagcgat ggttgacacc
    23281 aatactgatc cagatgatat cgatgtaatc atcccagcta acgatgacgc tatccgtgct
    23341 gttaaattga tcacagctaa attggctgac gctattatcg aaggacgtca aggtgaggat
    23401 gcagtagcag ttgaagcaga atttgcagct tcagaaactc aagcagattc aattgaagaa
    23461 atcgttgaag ttgtagaagg tgacaacaaa tagtaattac ctaggagggc ggggcttagc
    23521 ccggctctcc tattttcaaa aaatatagga gaattaaaat ggcagaaatt acagctaaac
    23581 ttgtaaaaga gttgcgtgaa aaatctggtg ccggtgttat ggacgctaaa aaagcgcttg
    23641 tagaaacaga cggtgacatc gaaaaagcga ttgaattgct tcgtgaaaaa ggtatggcta
    23701 aggcagctaa gaaagctgac cgtgttgctg cagaaggttt gactggtgtt tatgttaacg
    23761 gtaatgttgc agcagttatt gaagtaaacg ctgaaactga cttcgttgca aaaaacgctc
    23821 aattcgttga attggtaaat actacagcta aagtcattgc tgaaggaaaa cctgctaata
    23881 atgaagaagc tcttgctttg ataatgcctt caggtgaaac tcttgaagct gcatacgtat
    23941 ctgcaacagc aactatcgga gagaaaatct cattccgtcg ctttgcattg attgaaaaaa
    24001 cagacgcaca acactttgga gcataccaac ataacggtgg acgtatcggt gttatttcag
    24061 ttgttgaagg tggagacgaa gcacttgcta aacaattgtc aatgcacatc gcagcgatga
    24121 aaccaacagt tctttcttac aaagaattgg atgagcaatt cgttaaagat gagttggcac
    24181 aattgaatca cgttatcgac caagacaacg aaagccgtgc aatggttaat aaaccagctc
    24241 ttccacactt gaagtatgga tcaaaagctc aattaactga tgatgttatt gctcaagctg
    24301 aagctgacat caaagctgaa ttggctgcag aaggcaaacc agaaaaaatc tgggacaaaa
    24361 ttatcccagg taaaatggat cgcttcatgc ttgataatac taaagttgac caagcttaca
    24421 cacttcttgc acaagtttac atcatggatg acagcaagac agttgaagca taccttgaat
    24481 cagttaacgc ttcggtagtt gagtttgctc gctttgaagt tggtgaaggt atcgagaaag
    24541 ctgcaaacga cttcgaagct gaagttgcag ctacaatggc agcagccttg aataactaat
    24601 aatagaaagg aagcaaattg cttccttttt tgtatagggc ttagattttt cctgaaattg
    24661 ttttttcccc agtctcactc gattagaata attaaaacaa aaagccctat aataagggct
    24721 aagtgcattt aggagactat acttcaaatt catagtaagc gcgtcataac aaggtatcta
    24781 tcctaattcc ccaacttcca gtataccaga taaaaaataa ttatcggagg aaaggtattg
    24841 tactatgata attcaactaa gtgatttagg tcaagttcac cttgtttgtg gcaagacaga
    24901 tatgaggcag ggaatagact cattagccta tgtagttaaa acccactttg aattggatcc
    24961 tttctccgat caagtctttc tcttttgtgg tggacgtaaa gaccgcttta aagttcttta
    25021 ctgggatggt caaggatttt ggatattata taaacgcttt gagaacggaa aactgacttg
    25081 gtccagtaca gaaaaggatg tcaaagctct cacatctgaa caagtagatt ggctgatgaa
    25141 gggcttttct atcactcccc aaatatagtg gatttaaact agaatagtac acctctgctt
    25201 ctaaaacatt gttagaaatc gatttgacta tcctgttctt atttcatttt actataaatt
    25261 catcagaaag tcgtgatttc tattgaaatg aggactttct ttttatactc atctgttttc
    25321 aaaaagcatt ctagtccgtc tccgattaac gatggacttt atcacctcct tctccagtcc
    25381 ttgtatgaca tcttccaaag ttcgaaaggc tttattctta aatccacgtt tacgaatctc
    25441 tttccacact tgttcaatgg ggttcatctc tggtgtgtat ggaggaataa atgcaaagcc
    25501 aatattagtc ggaatcttta aggtacttga tttatgccat atagcattgt ccataacgag
    25561 taaaagataa tcatctggat aagcttgtga aagctcctat tcctaaagcc cctttagcgc
    25621 ataactttgg ctcagcttct attatcgctc acaccatcca tcagaagttt aatctgaagg
    25681 tacccaatta tcgccaagaa gaagattggg ctaggatggg tttaccaatc acacgtaagg
    25741 aaatctctaa ttggcatatc aagacgagtc aatactattt ggagcccctt tataacctct
    25801 tgcgagagag actattgact cagcccttac ttcatgcgga tgaaacttct tatagggtgc
    25861 tagagagtga tagtcagctg acttactatt ggactttttt gtctgggaaa gctgagaaac
    25921 aagggattac gctttaccat catgttctaa tcgatttgtt catatcttat ttcaatccat
    25981 tataaatagt gagaaatatc tatcctatct tctagaatgt cttccaaacg tggaaactct
    26041 cgtaaacaaa gaggttttag aggcctattt accatggact aaagttgtac aagaaaagtg
    26101 caaataagaa atctccagat taggaactat ccgtgagttc tctagtctgg agatttttca
    26161 atagacttcg ttattggacg gttacaattc ataaagtgct gtagagagat aacgttcacc
    26221 gttatctggt gctagggcaa ggactttttt acctgtacct aattttttgg caacctcgat
    26281 ggctccgtag atagctgcag ctgaggaaat ccctacaagg aagccttctt ttccaccaat
    26341 ttcacgtccg agtgcaagag cgtcatctga tgttacacga acgataccat cataggcttt
    26401 agtatcaagt gtatcaggaa taaatccagc tgagatacct tgaattttgt gaggaccagg
    26461 tttttcacca gatagaatag cagattcatc tgcttctact gcaaaaactt gaatgttaga
    26521 attttctgat ttgagtgcat gagaaacacc agaaatcgtt ccaccagtac ctactccagc
    26581 aacaaaggca tctaatccat ctttaccgaa agcagctagt atctcagctc ctgttgttct
    26641 ttcgtgtact tctggattag ctggattgtc aaattgaaga ggaaggaaac catcacgttc
    26701 agcagcgatt tcttgagcct tagcaatagc acctttcatt ccctcgctac caggagttag
    26761 gacgagttca gcaccataag cttggataat tttacgtcgt tctacactca tagtttcagg
    26821 cataacgatg acgactttat accctttagc agcacctacc catgaaagtc caataccggt
    26881 gtttccactt gttgcttcaa caatagtaga accaggtttc agaataccat cttgttcagc
    26941 tttttcaatc atgctaaggg caatacggtc ttttacagat gaaccaggat taaatgcttc
    27001 aagctttata tagacgtctg cagcaccttc tggcacgatg ttgttaagtt taacaatcgg
    27061 tgtttgaccg attaattcag taatgttgtt ataaatagac atatgaatac ctctttgtat
    27121 aagatatctt tagtataacg ctatccatac catttgtaaa atatagaaat cctatcgaag
    27181 cgataggatt tctatatatc atagatcaaa gtcattaatt ttcaagtgat tgcgttcagc
    27241 taattctagt aaataggtgt gaaggaaaac gatatcaggt ttcgtttgaa ttgcttcatt
    27301 ttctagtata ttttggtacg catcttttta ttttgctcgc taaagtagtt gtgagaatgt
    27361 caacgaaacg ctgtattatt cattgtttac actaagttta caggaacttc aacctctcgt
    27421 aaaccttggt cagttaaagt gacttttcca ttaaaaaact ccacaagtgc agctttaata
    27481 gtttcttttt cttctttatc aacataaatc atcgtatcga cttgatctgt aaagtttgta
    27541 tccagctcca tgagaccatg ttctttaagg aagttactgt actcttggta ctgagcataa
    27601 gacatttgaa tagcaatgcc agcctgttct tttatttcaa taataccaat ttctttgaca
    27661 gctaaggcga cactgccggc gtaagcacga attagtcctc cagcgcctag tttaatacca
    27721 ccaaagtagc gtgtcacgac cacacagaca ttggtgagat tgtgattttc tagtacccca
    27781 agcatgggaa caccagcagt accactaggc tcaccatcat cacttgtacg tttaatttca
    27841 ctacgttctc caataatgaa ggcagagcag ttatgtgtcg ctttgtagtg ttcttttttg
    27901 atggcagtaa tgaagtcacg agcctcttct tcgctataaa cacgcttggc atggcagata
    27961 aagcgagatt ttttgatttc ttcttggact tgaccgtcct ctttaattgt cctaaattcc
    28021 atgatttaat tgtactaaaa aatcatctaa agtgctagtt tttacgaata aagaagtatg
    28081 aaagtaaatt tagattatct cggtcgttta tttactgaga atgaattaac agaagaagaa
    28141 cgtcagttgg cggagaaact tccagcaatg agaaaggaga aggggaaact tttctgtcaa
    28201 cgctgtaata gtactattct agaagaatgg tatttgccca tcggtgctta ctattgtcga
    28261 gagtgcttgc tgatgaagcg agtcagaagt gatcaaactt tatactattt tccgcaggag
    28321 gattttccaa agcaagatgt tctcaaatgg cgcggccaat taactccttt tcaagagaag
    28381 gtgtcagagg gattgcttca agtagtagac aagcaaaagc caaccttagt tcatgcggta
    28441 acaggagctg gaaagacaga aatgatttat caagtagtgg ctaaagtgat caatgcgggt
    28501 ggtgcagtgt gtttggctag tcctcgcata gatgtttgtt tggagctgta caagcgcctg
    28561 caacaggatt tttcttgcgg gatagctttg ctacatggag aatcggaacc ttattttcga
    28621 acaccactag ttgttgcaac aacccatcag ttattgaagt tttatcaagc ttttgatttg
    28681 ctgatagtgg atgaagtaga tgcttttcct tatgttgata atcccatgct ttaccacgct
    28741 gtcaagaata gtgtaaagga gaatggattg agaatctttt taacagcgac ttcgaccaat
    28801 gagttagata aaaaggtccg tttaggagaa ctaaaaagac tgaatttacc gagacggttt
    28861 catggaaatc cgttgattat tccaaaacca atttggttat cggattttaa tcgctactta
    28921 gacaagaatc gtttgtcacc aaagttaaag tcctatattg agaagcagag aaagacagct
    28981 tatccgttac tcatttttgc ttcagaaatt aagaaagggg agcagttagc agaaatctta
    29041 caggagcaat ttccaaatga gaaaattggc tttgtatctt ctgtaacaga ggatcgatta
    29101 gagcaagtac aagcttttcg agatggagaa ctgacaatac ttatcagtac gacaatcttg
    29161 gagcgcggag ttaccttccc ttgtgtggat gttttcgtag tagaggccaa tcatcgtttg
    29221 tttaccaagt ctagtttgat tcagattggt ggacgagttg gacgaagcat ggatagaccg
    29281 acaggagatt tgcttttctt ccatgatggg ttaaatgctt caatcaagaa ggcgattaag
    29341 gaaattcaga tgatgaataa ggaggctggt ctatgaagtg cttgttatgt gggcagacta
    29401 tgaagactgt tttaactttt agtagtctct tacttctgag gaatgatgac tcttgtcttt
    29461 gttcagactg tgattctact tttgaaagaa ttggggaaga gaactgtcca aattgtatga
    29521 aaacagagtt gtcaacaaag tgtcaagatt gtcaactttg gtgtaaagaa ggagttgaag
    29581 tcagtcatag agcgattttt acttacaatc aagctatgaa ggattttttc agtcgctata
    29641 agtttgatgg agacttcctg ttaagaaaag ttttcgcttc atttttaagt gaggagttga
    29701 aaaagtacaa agagtatcaa tttgttgtaa ttcccctaag tcctgataga tatgctaata
    29761 gaggatttaa tcaggttgag ggcttggtag aggcagcagg ctttgagtat ctggatttat
    29821 tagagaaaag agaagagaga gccagttctt ctaaaaatcg ttcagagcgc ttggggacag
    29881 aacttccttt ctttattaaa agtggagtca ctattcctaa aaaaatccta cttatagatg
    29941 atatctatac tacaggagca actataaatc gtgttaagaa actgttggaa gaagctggtg
    30001 ctaaggatgt aaaaacattt tcccttgtaa gatgaggaaa aaatttgcaa aaatagaatg
    30061 aaagcgttat aataaaatca taaaaacaaa aatgtttaga aagaaggtac tcatatgatt
    30121 aaatatagta tccgtggtga aaacctagaa gtaacagaag caattcgtga ttatgtagtt
    30181 tctaaactcg aaaagatcga aaagtacttc caaccagaac aagagttgga tgcccgaatt
    30241 aacttaaaag tttatcgtga aaaaacggct aaagtggaag taacgattcc gcttggatct
    30301 attactctcc gcgcagaaga tgtatctcaa gatatgtatg gttcaattga ccttgtaact
    30361 gataaaattg aacgtcagat tcgtaaaaat aaaacaaaaa tcgagcgtaa aaataaaaat
    30421 aaggtagcaa ctggtcaatt atttacagat gctttggtgg aagattcaaa tgttgtccag
    30481 tctaaagttg ttcgttcaaa acaaattgat ttaaaaccaa tggatttgga agaagcaatt
    30541 ctacaaatgg atttattggg gcatgatttc tttatctatg tggatgttga agatcagaca
    30601 accaatgtga tttatcgtcg tgaggatggc gaaattggtt tgttagaggt taaagaatct
    30661 taatttcaat atgtgtaaag gtaggtttac tgaattgtaa actacctttt tcttataatt
    30721 gatagaatta ctgattacac ttttaaaaag tcgctttttg aggggtatta tggtataatg
    30781 ggtgccaaga ggttttgaat gaaaaaattt tatgtaagtc caatttttcc tattctagta
    30841 ggattgattg cgtttggagt cttatccact ttcattattt ttgttaataa taatctgttg
    30901 acggttttaa ttttgtttct ttttgtagga ggctatgttt ttttatttaa gaaactgaga
    30961 gtgcattata caaggagtga tgtagaacag atacagtatg taaaccacca agcggaagaa
    31021 agtttgacat ctctattgga acaaatgcct gtaggtgtta tgaaattgaa tttatcttct
    31081 ggagaggttg agtggtttaa tccctatgct gaattgattt tgaccaagga agatggtgat
    31141 tttgatttag aagctgttca aacgattatc aaggcttcag taggaaatcc gtctacttat
    31201 gccaagcttg gtgagaagcg ttatgctgtt catatggatg cttcttccgg tgttttgtat
    31261 tttgtagatg tatccaggga acaagccata acagatgaat tggtaacaag tcgaccagtg
    31321 attgggattg tctctgtgga taattatgat gatttggagg atgcaacttc tgagtcagat
    31381 attagtcaaa tcaatagttt tgtagctaat tttatatcag agttttcaga aaaatacatg
    31441 atgttttctc gtcgggtaag tatggatcga ttttatctat ttactgacta cacggtgctt
    31501 gagggcttga tgaatgataa attttctgtt attgatgctt tcagagaaga gtcgaaacag
    31561 agacagttgc ccttgacctt aagtatggga ttttcttatg gcgatggaaa tcatgatgag
    31621 atagggaaag ttgctttgct caatttgaac ttggctgaag tacgtggtgg cgaccaggtg
    31681 gttgttaagg aaaacgacga aacgaaaaat ccagtttatt ttggtggtgg gtctgctgct
    31741 tcaatcaagc gtacacggac tcgtacgcgc gctatgatga cagctatttc agataagatt
    31801 cggagtgtag atcaggtttt tgtagtcggt cacaaaaatt tagacatgga tgctttgggc
    31861 tctgctgtag gtatgcagtt gttcgccagc aatgtgattg aaaatagcta tgctctttat
    31921 gatgaagaac aaatgtctcc agatattgaa cgagctgttt cattcataga aaaagaagga
    31981 gttacgaagt tgttgtctgt taaggatgca atggggatgg tgaccaatcg ttctttgttg
    32041 attcttgtag accattcaaa gacagcctta acattatcaa aagaatttta tgatttattt
    32101 acccaaacca ttgttattga ccaccataga agggatcagg attttccaga taatgcggtt
    32161 attacttata tcgaaagtgg tgcaagtagt gccagtgagt tggtaacgga attgattcag
    32221 ttccagaatt ctaagaaaaa tcgtttgagt cgtatgcaag caagtgtctt gatggctggt
    32281 atgatgttgg atactaaaaa tttcacctcg cgagtaacta gtcggacatt tgatgttgct
    32341 agctatctca gaacgcgcgg aagtgatagt attgctatcc aggaaatcgc tgcgacagat
    32401 tttgaagaat atcgtgaggt caatgaactt attttacagg ggcgtaaatt aggttcagat
    32461 gtactaatag cagaggctaa ggacacgaaa tgctatgata cagttgttat tagtaaggca
    32521 gcagatgcca tgttagccat gtcaggtatt gaagcgagtt ttgttcttgc gaagaataca
    32581 caaggattta tctctatctc agctcgaagt cgtagtaaac tgaatgtaca acggattatg
    32641 gaagagttag gcggtggagg ccactttaat ttggcagcag ctcaaattaa agatgtaacc
    32701 ttgtcagaag caggtgaaaa actgacagaa attgtattaa atgaaataaa ggaaaaggag
    32761 aaagaagaat gaaagtaatc tttttagcag atgttaaagg aaaaggtaaa aaaggcgaaa
    32821 ttaaggaagt accaacaggg tatgcgcaaa actttcttat caaaaagaat ctagccaaag
    32881 aagcgactgc tcaagctgta ggtgaacttc gtggtaaaca aaaatcggaa gaaaaagctc
    32941 acgctgagat gattgcagaa ggaaaagcaa ttaaagcaca acttgaagca gaagaaactg
    33001 ttgtagaatt tgttgaaaaa gttggtccag atggtcgtac ctttggttct attaccaata
    33061 agaagattgc agaagaattg caaaagcaat ttggaattaa gattgataaa cgtcatattc
    33121 aagtacaagc tccgattcga gcggttggtt tgattgatgt gccagtgaaa atctatcaag
    33181 atatcacaag tgtaatcaat cttcgtgtga aagaaggata agtttacacc ttcttgacaa
    33241 gattgtaaaa ggaagggaag tctgatggca gaagtagaag agttacgagt acaacctcaa
    33301 gatatcttag ctgagcaatc cgttttaggg gctatcttta ttgatgagag taaacttgtt
    33361 tttgtgcgag aatacattga gtctcgggac ttttttaagt atgcccatcg tttgattttc
    33421 caagccatgg tcgatttatc cgatcgtggt gatgccatag atgcaacaac ggttcgtact
    33481 atccttgata atcaaggtga tttacagaat attggtggct tgtcttactt ggttgagatt
    33541 gttaattctg tgccaacttc tgctaatgcg gagtattatg ctaagattgt tgcagaaaaa
    33601 gcaatgctac gtcgtttaat tgccaagttg acagagtctg tcaaccaagc ttacgaagcg
    33661 tcacaaccag ctgatgaaat tattgctcag gcagaaaaag ggttgattga tgtcagtgaa
    33721 aatgcaaatc gaagcgggtt taagaacatt cgagatgtgt tgaatctcaa ctttggaaat
    33781 ctggaagctc gctcgcaaca aacgaccgat attacaggta ttgcgacagg ttatcgtgat
    33841 ttggatcata tgacaacagg acttcatgag gaggagttga ttatcttagc agctcgtcca
    33901 gcagttggta agacagcatt tgccttgaat atcgctcaga atattgggac taagttggac
    33961 aaaacggttg ctattttttc actcgaaatg ggtgcggaaa gcttggtaga ccgtatgtta
    34021 gctgcagaag gcttggtgga gtcacattct atccgtacag ggcaattgac agatgaggag
    34081 tggcaaaaat atactattgc tcagggtaat ctagctaacg ccagtatcta tatcgatgat
    34141 acgccaggta ttcggattac agagattcgt tctcgttctc gtaaattggc tcaagaaact
    34201 ggaaatcttg gtttgatttt gatagactat ttgcaactta tcacgggaac tggtcgagaa
    34261 aatcgtcaac aagaagtttc tgaaatttct cgtcagttga aaatactagc caaggaattg
    34321 aaggttccag taatcgctct gagtcagctt tctcgtggtg tagaacaacg tcaggacaag
    34381 agaccggtct tgtctgatat tcgtgaatct gggtctattg agcaggacgc tgatatcgta
    34441 gcttttctct atcgcgatga ctactatgaa cgtggtggtg aagaagagga gggtatccca
    34501 aataataagg tggaagttat tatcgagaaa aaccgtagtg gagctcgtgg aacagtggaa
    34561 ttgattttcc aaaaagaata caataaattt tcaagtatct caaagaggga ggcataagat
    34621 gtcagatgca tttacagatg tagccaagat gaaaaaaatc aaagaagaaa tcaaggcaca
    34681 tgagggacaa gtcgtagaaa tgactttgga gaatggtcgt aagcgccaaa aaaatagatt
    34741 gggtaagcta attgaagttt atccatccct atttattgtg gagtttgggg atgtggaagg
    34801 agataaacaa gttaatgttt acgttgaatc ctttacttac tcagatattc ttacagaaaa
    34861 gaatttgatt cattatcttg actaaagtga gaaattttct cactttttct tttttctccg
    34921 aataatttag gtgaaggcaa tcatcgcttt atattatttt ttaaggagga agaatgaaaa
    34981 ttttaccgtt tatagcaaga ggaacaagtt attacttgaa gatgtcagtt aaaaagcttg
    35041 ttcctttttt agtagtagga ttgatgctag cagctggtga tagtgtctat gcctattcca
    35101 gaggaaatgg atcgattgcg cgtggggatg attatcctgc ttattataaa aatgggagcc
    35161 aggagattga tcagtggcgc atgtattctc gtcagtgtac ttcttttgta gcctttcgtt
    35221 tgagtaatgt caatggtttt gaaattccgg cagcttatgg aaatgcgaat gaatggggac
    35281 atcgtgctcg tcgggaaggt tatcgtgtag ataatacacc gacgattggt tccattactt
    35341 ggtctactgc aggaacttat ggtcatgttg cctgggtgtc aaatgtaatg ggagatcaga
    35401 ttgagattga ggaatataac tatggttata cagaatccta taataaacga attataaaag
    35461 caaacacgat gacaggattt attcatttta aagatttgga tggtggcagt gttgggaata
    35521 gtcaatcctc aacttcaaca ggcggaactc attattttaa gaccaagtct gctattaaaa
    35581 ctgaacccct agttagtgca actgtgattg attactatta tcctggagag aaggttcatt
    35641 atgatcaaat tctcgaaaaa gacggctaca agtggttgag ttatactgcc tataatggaa
    35701 gctatcgtta tgttcaattg gaggctgtga ataaaaatcc tctaggtaat tctgttcttt
    35761 cttcaacagg tggaactcat tattttaaga ccaagtctgc tatcaaaact gaacccctag
    35821 ttagtgcaac tgtgattgat tactattatc ctggggagaa ggttcattat gatcagatac
    35881 ttgaaaaaga cggctacaag tggttgagtt atacggctta taacggaagt cgtcgctata
    35941 tacagctaga gggagtgact tcttcacaaa attatcagaa tcaatcagga aatatctcta
    36001 gctatggatc caataatagt tcaactgtcg gttggaagaa aataaatggt agttggtatc
    36061 atttcaaatc aaatggttct aaatcaacag gatggctgaa agatggttct agctggtatt
    36121 atttgaaatt atctggtgaa atgcagacag gatggttaaa ggagaatggc tcgtggtatt
    36181 atctgggtag ttcaggggca atgaaaacag gctggtacca ggtctctggt aagtggtatt
    36241 attcttactc ttcaggcgcc ttagctgtta atacgacggt ggatggctac agagtaaaca
    36301 gtgatggaga acgagtatag aaaattggag taggagaaat ttcctgctct tttctgtaag
    36361 cagtttcctt gacatttttc ttattttgcg ctatcatata tacatataat atatgggagt
    36421 ctgtgtacag tctgagagga agtgttaaac ttcgaccgca cctgatctgg gtaatgccag
    36481 cggagggaac gatacttagt ctaattttgc accttttcca tgtatggtaa aggtttttct
    36541 ttttttaaaa aggaaaacga gaagaggagg ttcttatgaa agcaagcatt gccttgcaag
    36601 ttttacccct agcacagggg attgatcgga tagctgttat tgatcaggtc attgcttatc
    36661 tgcaaactca agaagtgacg atggtagtga caccatttga aacggtcttg gaaggggagt
    36721 ttgatgagct tatgcgcatt ctaaaagaag cgctggaagt ggcagggcag ggggcagaca
    36781 atgtcttttc caatgtcaaa ataaatgtag gagagatttt aagtattgat gagaaacttg
    36841 agaagtatac tgagacgaca cattagtcta ttgggctttc tcggagtctt gtcaatctgg
    36901 cagttagcag gttttcttaa acttctcccc aagtttatcc tgccgacacc tcttgaaatt
    36961 ctccagccct ttgttcgtga cagagaattt ctctggcacc atagctgggc gaccttgaga
    37021 gtggctttac tggggctgat tttgggagtt ttgattgcct gtcttatggc tgtactcatg
    37081 gatagtttga cttggttcaa tgacctgatt taccctatga tggtggtcat tcagaccatt
    37141 ccgaccattg ccatagctcc tatcctggtc ttgtggctag gttatgggat tttgcccaag
    37201 attgtcttga ttatcttaac aacaaccttt cccatcatcg ttagtatttt ggacggtttt
    37261 aggcattgtg acaaggatat gctgaccttg tttagtctga tgcgagcaaa cccttggcaa
    37321 atcctgtggc attttaaaat cccggttagc ctgccttact tttatgcagg tctgagggtc
    37381 agtgtctcct acgcctttat cacaactgtg gtatctgagt ggttgggagg ttttgagggg
    37441 cttggtgttt atatgattca gtccaagaaa ttgtttcagt atgataccat gtttgccatt
    37501 attattctgg tgtcgattat cagtcttttg ggtatgaagc tggtcgatat cagtgaaaaa
    37561 tatgtgatta aatggaaacg tacttaaaat agagcgtttt tgaaaaagaa aagaggaaat
    37621 caaaatgaag aaaacatgga aagtgttttt aacgcttgta acagctcttg tagctgttgt
    37681 gcttgtggcc tgtggtcaag gaactgcttc taaagacaac aaagaggcag aacttaagaa
    37741 ggttgacttt atcctagact ggacaccaaa taccaaccac acagggcttt atgttgccaa
    37801 ggaaaaaggt tatttcaaag aagctggagt ggatgttgat ttgaaattgc caccagaaga
    37861 aagttcttct gacttggtta tcaacggaaa ggcaccattt gcagtgtatt tccaagacta
    37921 catggctaag aaattggaaa aaggagcagg aatcactgcc gttgcagcta ttgttgaaca
    37981 caatacatca ggaatcatct ctcgtaaatc tgataatgta agcagtccaa aagacttggt
    38041 tggtaagaaa tatgggacat ggaatgaccc aactgaactt gctatgttga aaaccttggt
    38101 agaatctcaa ggtggagact ttgagaaggt tgaaaaagta ccaaataacg actcaaactc
    38161 aatcacaccg attgccaatg gcgtctttga tactgcttgg atttactacg gttgggatgg
    38221 tatccttgct aaatctcaag gtgtagatgc taacttcatg tacttgaaag actatgtcaa
    38281 ggagtttgac tactattcac cagttatcat cgcaaacaac gactatctga aagataacaa
    38341 agaagaagct cgcaaagtca tccaagccat caaaaaaggc taccaatatg ctatggaaca
    38401 tccagaagaa gctgcagata ttctcatcaa gaatgcacct gaactcaagg aaaaacgtga
    38461 ctttgtcatc gaatctcaaa aatacttgtc aaaagaatac gcaagcgaca aggaaaaatg
    38521 gggtcaattt gacgcagctc gctggaatgc tttctacaaa tgggataaag aaaatggtat
    38581 ccttaaagaa gacttgacag acaaaggctt caccaatgaa tttgtgaaat aatgacagaa
    38641 attagactag agcacgtcag ttatgcctat ggtcaggaga ggattttaga ggatatcaac
    38701 ctacaggtga cttcaggcga agtggtttcc atcctaggcc caagtggtgt tggaaagacc
    38761 accctcttta atctaatcgc tgggatttta gaagttcagt cagggagaat tgtccttgat
    38821 ggtgaagaaa atccccaggg gcgcgtgagt tatatgttgc aaaaggatct gctcttggag
    38881 cacaagacgg tgcttggaaa tatcattctg cccctcttga ttcaaaaggt ggataaggca
    38941 gaagctattt cccgagcgga taaaattctt gcgaccttcc agctgacagc tgtaagagac
    39001 aagtatcctc atgaacttag cggtgggatg cgccagcgtg tagccttact ccggacctac
    39061 ctttttgggc acaagctctt tctcttagat gaggccttta gcgccttgga tgagatgaca
    39121 aagatggaac tccacgcttg gtaccttgag attcacaagc agttgcagct aacaaccttg
    39181 attattactc acagtatcga ggaggccctc aatctcagtg accgcatcta tatcttgaaa
    39241 aatcgccctg ggcagattgt ttcagaaatt aaactagatt ggtctgaaga tgaggacaag
    39301 gaagttcaaa agattgccta caaacgtcaa atcttggcag aattaggctt agataagtag
    39361 aaaaataggg agttggtgaa gattatcctt taccagcgcc ctttttcttt taaaaatgag
    39421 aaaatttcgg tataatagtc aaacaaggtc aaggtttaaa gagagaggtg ggtttgttat
    39481 gagatttaaa aatacatcgg atcatattga ggcctacatc aaggcgattt tagatcaatc
    39541 tggtatcgtg gagttgcaac ggagtcagtt ggcagatacc tttcaggttg ttcctagtca
    39601 gattaactac gtgatcaaga cacgctttac ggaaagtaga ggctacttgg ttgaaagtaa
    39661 gcgtggtggc ggaggctaca ttcgtatagg acggattgag ttttctagtc atcatgaaat
    39721 gctccgggag ctgctttact cgattggtga gcgagtcagt caagaaattt atgaggatat
    39781 tctccagctt ttggttgagc aggaattgat gaccaagcag gagatgaatt tgctagaatc
    39841 agtagctttg gatcgcgttt taggagaaga agctccagtt gttcgagcaa acatgctacg
    39901 tcagatcata caagaggtag atagaaaagg gaagtaagat gaactattca aaagcattga
    39961 atgaatgtat cgaaagtgcc tacatggttg ctggacattt tggagctcgt tatctagagt
    40021 cgtggcactt gttgattgcc atgtctaatc acagttatag tgtagcaggg gcaactttaa
    40081 atgattatcc gtatgagatg gaccgtttag aagaggtggc tttggaactg actgaaacgg
    40141 actatagcca ggatgaaacc tttacggaat tgccgttctc ccgtcgtttg caggttcttt
    40201 ttgacgaagc agagtatgta gcgtcagtgg tccatgctaa ggtgctaggg acagagcacg
    40261 tcttctatgc gattttgcat gatggcaatg ccttggcaac tcgtatcttg gagagggctg
    40321 gtttttctta tgaagacaag aaagatcagg tcaagattgc tacccttcgt cgaaatttag
    40381 aagaacgtgc aggctggact cgtgaagatc tcaaggcttt acgccaacgc catcgtacag
    40441 tagctgacaa gcaaaattct atggccaata tgatgggcat gccgcagact cctagtggtg
    40501 gtctcgagga ctatacgcat gatttgacag agcaagcgcg ttctggcaag ttagaaccag
    40561 tcatcggtcg ggacaaggaa atctcacgta tgattcaaat attgagccgt aagactaaga
    40621 acaaccctgt cttggttggg gatgctggtg tcgggaaaac agctctggcc cttggtcttg
    40681 ctcagcgtat tgctagtggt gacgtgcctg cggaaatggc taagatgcgc gtgttagaac
    40741 ttgatttgat gaatgtcgtt gcagggacac gcttccgtgg tgactttgaa gaacgcatga
    40801 ataatatcat caaggatatt gaagaagatg gccaagtcat cctctttatc gatgaactcc
    40861 acaccatcat gggttctggt agcgggattg attcgactct ggatgcggcc aatatcttga
    40921 aaccagcctt ggcgcgtgga actttgagaa cggttggtgc caccactcag gaagaatatc
    40981 aaaaacatat cgaaaaagat gcggctcttt ctcgtcgttt cgctaaagtg acgattgaag
    41041 aaccaagtgt ggcagatagt atgactattt tacaaggttt gaaggcgact tatgagaaac
    41101 atcaccgtgt acaaatcaca gatgaagcgg ttgaaacagc ggttaagatg gctcatcgtt
    41161 atttaaccag tcgtcacttg ccagactctg ctatcgacct cttggacgaa gcagcagcaa
    41221 cagtgcaaaa taaggcaaag tatgtaaaag cagacgattc agatttgagt ccagctgaca
    41281 aggccctgat ggatggcaag tggaaacagg cagcccagct aatcgcaaaa gaagaggaag
    41341 tacctgtcta caaagacttg gtgacagagt ctgatatttt gaccaccttg agtcgcttgt
    41401 caggaatccc agttcaaaaa ctgactcaga ctgacgctaa gaaatatcta aatcttgaag
    41461 cagaactcca taaacgggtt atcggtcaag atcaagctgt ttcaagtatt agtcgtgcga
    41521 ttcgccgtaa tcagtcagga attcgcagtc acaagcgtcc gatcggttcc tttatgttcc
    41581 taggacccac aggtgtcggt aagaccgagt tggccaaggc tttggcagaa gttctctttg
    41641 atgacgaatc agcccttatc cgctttgata tgagtgagta tatggagaaa ttcgcagcca
    41701 gccgtctaaa tggagctcct ccaggctatg tgggttacga agaaggtggg gagttgacag
    41761 agaaggttcg caataaaccc tattccgttc tcctctttga tgaggtagag aaggctcacc
    41821 cagatatctt taatgttctc ttgcaggttc tggatgacgg tgtcttgaca gatagcaagg
    41881 gacgcaaggt cgatttttca aataccatta tcattatgac atcgaaccta ggtgcgactg
    41941 cccttcgtga tgataagact gttggttttg gggctaagga tattcgtttt gaccaggaaa
    42001 atatggaaaa acgcatgttt gaagaactga aaaaagctta tagaccggaa ttcatcaacc
    42061 gtattgatga gaaggtggtc ttccatagcc tatctagtga ccatatgcag gaagtggtga
    42121 agattatggt caagccttta gtggcaagtt tggctgaaaa aggcattgac ttgaaattac
    42181 aagcttcagc tctgaaattg ttagcaaatc aaggatatga cccagagatg ggagctcgcc
    42241 cacttcgcag aaccctgcaa acagaagtgg aggacaagtt ggcagaactt cttctcaagg
    42301 gagatttagt ggcaggcagc acacttaaga ttggtgtcaa agcaggccag ttaaaatttg
    42361 atattgcata aaagaataaa agtaccagca tctgaccata agtcacagtg gagtgaaact
    42421 caatgaaaat caaagagcaa actaggcagc tagccgcagg ttgctcaaaa cactggtttg
    42481 aggttgcaga tagagctgac gtggtttgaa gagattttcg aagagtataa gctgcaagat
    42541 gaatgatttt cttgtattga cgttgttgtt gacaaaaagt agcggataaa tgaaatccat
    42601 tccattatca tagatgatag gctggtagga aattttcaaa tagcatacag gaaatagatg
    42661 tatggagttc tggtagtaga aagggagaga gatgaacatt ttagttgcag atgacgagga
    42721 aatgattaga gaaggaattg cagcatttct gacagaagag ggttatcatg tcattatggc
    42781 taaggatgga caagaggtct tggaaaaatt tcaagatctc cctatccatc tcatggtact
    42841 ggatttaatg atgcctagga agagtggttt tgaagtgtta aaagaaatca atcaaaagca
    42901 cgatattcct gtcatcgtct tgagtgctct gggagatgaa actactcagt cacaggtatt
    42961 tgatctctat gctgatgatc atgtgacaaa acctttttct ttggtactgc ttgtcaagcg
    43021 tattaaggcg cttatcagac gttactacgt catagaggat ctttggcgat atcaggatgt
    43081 aacagtggat tttacctctt acaaagcaca ttataaaaat gaagaaattg atctcaaacc
    43141 aaaggaatta ctggtactaa agtgtttgat tcagcataaa aatcaagttt taagtagaga
    43201 gcagatattg gaagaaattt caaaagatgt agctgattta ccttgtgata gggtcgttga
    43261 tgtctatatt cgtactcttc gcaaaaaatt agctttagat tgtatcgtga ctgtgaaaaa
    43321 tgttgggtat aagattagct tatgataaaa aatcctaaat tattaaccaa gtctttttta
    43381 agaagttttg caattctagg tggtgttggt ctagtcattc atatagctat ttatttgacc
    43441 tttccttttt attatattca actggagggg gaaaagttta atgagagcgc aagagtgttt
    43501 acggagtatt taaagactaa gacatctgat gaaattccaa gcttactcca gtcttattca
    43561 aagtccttga ccatatctgc tcaccttaaa agagatattg tagataagcg gctccctctt
    43621 gtgcatgact tggatattaa agatggaaag ctatcaaatt atatcgtgat gttagatatg
    43681 tctgttagta cagcagatgg taaacaggta accgtgcaat ttgttcacgg ggtggatgtc
    43741 tacaaagaag caaagaatat tttgcttttg tatctcccat atacattttt ggttacaatt
    43801 gctttttcct ttgttttttc ttatttttat actaaacgct tgctcaatcc tcttttttac
    43861 atttcagaag tgactagtaa aatgcaagat ttggatgaca atattcgttt tgatgaaagt
    43921 aggaaagatg aagttggtga agttggaaaa cagattaatg gtatgtatga gcacttgttg
    43981 aaggttattc atgagttgga aagtcgtaat gagcaaattg taaaattgca aaatcaaaag
    44041 gtttcctttg tccgcggagc atcacatgag ttgaaaaccc ctttagccag tcttagaatt
    44101 atcctagaga atatgcagca taatattgga gattacaaag atcatccaaa atatattgca
    44161 aagagtataa ataagattga ccagatgagc cacttattgg aagaagtact ggagtcttct
    44221 aaattccaag agtggacaga gtgtcgtgag accttgactg ttaagccagt tttagtagat
    44281 attttatcac gttatcaaga attagctcat tcaataggtg ttacaattga aaatcaattg
    44341 acagatgcta ccagggtcgt catgagtctt agggcattgg ataaggtttt gacaaacctg
    44401 attagtaatg caattaaata ttcagataaa aatgggcgtg taatcatatc cgagcaagat
    44461 ggctatctct ctatcaaaaa tacatgtgcg cctctaagtg accaagaact agaacattta
    44521 tttgatatat tctatcattc tcaaatcgtg acagataagg atgaaagttc cggtttgggt
    44581 ctttacattg tgaataatat tttagaaagc tatcaaatgg attatagttt tctcccttat
    44641 gaacacggta tggaatttaa gattagcttg tagacagatt agttttttat taaagttcac
    44701 atagggttaa cataagtgta ttattcttta tatagataaa aatatagata aaagaaagga
    44761 tactaatatg gtattagcga ttattttagt aatattcctt attcgattga tttttttaaa
    44821 gcgttcgata gagaatgaga aacgaatcct tagcaatggc gggaaagaat ttggagttga
    44881 gaatacaaaa cgattaacgt tggctcatat tgttttttat ctctcttgcc tcgttgaagc
    44941 aatggtgcat aagacaatgt ttgatggtat gagcatggtt ggtttagtct tgcttatttt
    45001 ttctatgctg atgttgatgt tggtgattca cttgttggga gatatttgga cagtgaagct
    45061 tatgcttgtc aataatcaca aatatgtaga tcatatctta tttaggacag taaaacatcc
    45121 taattacttt ttaaatatcc ttcctgagtt gattggcttg accttgttga gtcatgccta
    45181 tatgactttt gttttagttt ttccagttta tgcagttatt ttgtatcgac gaatagctga
    45241 agaggaaaag ctattacatg aagttataat cccaaatgga agcataaaga gataaataca
    45301 aaattcgatt tatatacagt tcatattgaa gtgatatagt aaggttaaag aaaaaatata
    45361 gaaggaaata aacatgtttg catcaaaaag cgaaagaaaa gtacattatt caattcgtaa
    45421 atttagtatt ggagtagcta gtgtagctgt tgccagtctt gttatgggaa gtgtggttca
    45481 tgcgacagag aaggaggtaa ctacccaagt acccacttat tctaatatgg caaagacaga
    45541 acataggaaa gctgctaaac aagtcgtcga tgaatatata gaaaaaatgt tgagggagat
    45601 tcaattagat agaagaaaac atacccaaaa tttcgccttc aacatgaagt tgagcgcaat
    45661 taaaacggag tatttgtatg gattaaaaga gaagtcggaa gctgagttgc cgtcatcgga
    45721 agctgagttg ccgtcagaag taaaagcaaa gttagacgca gcttttgagc agtttaaaaa
    45781 agatacattg aaactaggag aaaaggtagc agaagctgag aagaaggttg cagaagctga
    45841 gaaaaaagcc aaggctcaaa aagaagaaga tcgccgtaac tacccaacca ttacttacaa
    45901 aacgcttgac cttgaaattg ctgagtccga tgtggaagtt aaaaaagcgg agcttgaact
    45961 attgaaagag gaagctaaaa ctcgaaacaa ggacacaatt aagcaagcaa aagcgaaagt
    46021 tgagagtaaa caagctgagg ctacaaggtt agaaaaaatc aagacagatc gtaaaaaagc
    46081 agaagaagaa gctaaacgaa aagcagcaga agaagataaa gttaaagata aactaaagag
    46141 gcggacaaaa cgagcagttc ctggagagcc agcaacacct gataaaaaag aaaatggtgc
    46201 gaagtcttca gattctagcg taggtgaaga aactcttcca agcccatccc tgaaaccaga
    46261 aaaaaaggtt gcagaagctg agaagaaggt tgcagaagct gagaaaaaag ccaaggatca
    46321 aaaagaagaa gatcgccgta actacccaac caatacttac aaaacgcttg aacttgaaat
    46381 tgctgagtcc gatgtgaaag ttaaagaagc ggagcttgaa ctagtaaaag aggaagctaa
    46441 ggaatctcga aacgaggaaa aagttaagca agcaaaagcg aaagttgaga gtaaacaagc
    46501 tgaggctaca aggttagaaa aaatcaagac agatcgtaaa aaagcagaag aagaagctaa
    46561 acgaaaagca gcagaagaag ataaagttaa agaaaaacca gctgaacaac cacaaccagc
    46621 gccggctcct caaccagaaa aaccagctcc agctccaaaa ccagagaatc cagctgaaca
    46681 accaaaagca gaaaaaccag ctgatcaaca agctgaagaa gactatgctc gtagatcaga
    46741 agaagaatat aatcgcttga ctcaacagca accgccaaaa actgaaaaac cagcacaacc
    46801 atctactcca aaaacaggct ggaaacaaga aaacggtatg tggtacttct acaatactga
    46861 tggttcaatg gcgacaggat ggctccaaaa caatggctca tggtactacc tcaacagcaa
    46921 tggcgctatg gcgacaggat ggctccaaaa caatggttca tggtactatc taaacgctaa
    46981 tggttcaatg gcaacaggat ggctccaaaa caatggttca tggtactacc tcaacgctaa
    47041 tggttcaatg gcgacaggat ggctccaata caatggttca tggtactacc tcaacgctaa
    47101 tggtgatatg gcgacaggat ggctccaaaa caatggctca tggtactacc taaacgctaa
    47161 tggtgatatg gcgacaggtt gggtgaaaga tggagatacc tggtactatc ttgaagcatc
    47221 aggtgctatg aaagcaagcc aatggttcaa agtatcagat aaatggtact atgtcaatgg
    47281 ctcaggtgcc cttgcagtca acacaactgt agatggctat ggagtcaatg ccaatggtga
    47341 atgggtaaac taaacctaat ataactagtt aatactgact tcctgtaaga actctttaaa
    47401 gtattcccta caaataccat atcctttcag tagataatat acccttgtag gaagtttaga
    47461 ttaaaaaata actctgtaat ctctagccgg atttatagcg ctagagacta cggagttttt
    47521 ttgatgagga aagaatggcg gcattcaaga ggctctttaa gagagttacg ggttttaaac
    47581 tattaagcct tctccaattg caagagggct tcaatctctg ccagggtgct agcttgcgaa
    47641 atggctccac ggagtttggc agcgccagat gttccacgga ggtagtgagg agcgaggccg
    47701 cggaattcac gaactgcgac gttttctcct ttgaggttaa tcaatcgttt caagtgttcg
    47761 taggcgatct tcatcttgtc ttcaaaggtc aaatcaggta ggatttctcc tgtttcaaag
    47821 taatggttga tttggttgaa gaggtaagga tttcccatgg cagctcggcc aatcatgact
    47881 gcgtcagcac caacttcttc gatgcgttgc ttggcttctt ggacagtacg gatatcaccg
    47941 ttggcgatga atggaatctt ggttagagct tgggcaacct tgtaaagggt ctcaaggtct
    48001 gcgtggccag tatacatttg ttcacgggta cggccatgca tggcgagggc agaaacacct
    48061 gcagcctcag cagcgagggc attttctact gccagagatg ggtccgccca gccggtacgc
    48121 attttgacag taagtgggat atcaaggaca gactggacct tgttgatgat agagtagatc
    48181 ttgtcaggat ccttgagcca catagctcca gcttcgttct tcacgatttt gttgacaggg
    48241 cagcccatgt tgatatcgac gatatcggtc ttggtgtttt cttggatgaa ttctgctgcg
    48301 cgtgctaggc tgtcttcatc gctaccaaaa agttggatag agacagggtt ttcgccctca
    48361 tcgatatgaa gcatgtgcag ggttttttcg ttgttgtatt ggattccctt gtcagagacc
    48421 atttccatta caacgagtcc agctccgagc tcttttgcga tggtacgaaa ggctgagttg
    48481 gtcacgccag ccataggcgc taaaacggta cgattgggaa tctcaatatt gccaatcata
    48541 aaaggtgtat taagatttgt cacgaatgag ttcctccagg tccttttcat caaagttgta
    48601 agtagtttgg cagaattgac aagtgatttc tgccccgtgg tcttcctctt tcatttcctg
    48661 taagtctgag cttggaaggc tggcaagagc gttcataaag cgttcatggc tacagtcaca
    48721 ttggaaacgg atttcttctt cagaaagacg cttgtaggct tcgtccccgt agatagcctt
    48781 gaggagggct tcgatatggt cgtcgctttc gagaagagta gagatagctg gcatttcttg
    48841 gatgcgtttt tcaaagcgag caatctcttc tttcttggct cctggcaaga cttgaactag
    48901 gaaaccacct gcaaccttga ccttgtcttc ctcgtccaaa aggacattga ggccgaccgc
    48961 tgaaggcgtt tgttggcttt cagtaaggta aaaggcaagg tcttcaccga tttctccaga
    49021 gatgagggga gttatagagt tgtaaggatt tccagcaccg tagtctgtga taacgaggaa
    49081 ttgaccattt ccaacaaaag gtccgactag gacttcacca gtcgcagtct ttttgatgtc
    49141 aacaccagga ttttgaacat agcctttgac gttccccttg gtatcagcga cggtgataat
    49201 agcacctaga gagctagagc ccaacacctt aactgtaagt ttggtatttc ctttttcatt
    49261 ggctgcgaga atctggctgg cgataagagt tcgaccaagc gctacagttg agctagcttg
    49321 ggtttgatgt ttttcttgag cagtgcggac ggtttccgtg ctatcaagga caaaagcacg
    49381 aaaggctccg ctttctgata tagttttaat aattttatcc atagctacta ttttagcata
    49441 aaaatgccca aagggggagc cgtgtgttta ctgattttca ggataatgga ccaggaaatc
    49501 agcatgaaaa taaaaagaga aacagattat ttcagcattt gtaagattta tgctatgctt
    49561 aaggtagaaa atgaaagggg taacaaatgt atttaggaga tttgatggag aaagccgagt
    49621 gtggtcaatt ttcaatcctt tcctttctat tacaagagtc tcagacgacc gtcaaggctg
    49681 taatggaaga aacaggattt tcaaaagcaa ccctaaccaa atatgtcacc ctgctcaatg
    49741 acaaggcttt ggatagtggc ttagagctgg ctattcactc agaagatgaa aatctgcgtc
    49801 tgtctatcgg tgcagctacc aaggggagag atattcggag cttgtttttg gagagtgctg
    49861 ttaaatacca gattttggtt tatcttctct accaccaaca gtttttagcc catcagctgg
    49921 ctcaagaatt ggtgattagc gaggctacgc ttggtcgtca cttggctggt ttaaatcaga
    49981 ttttgtcaga atttgattta tccatccaaa atggacgttg gcgaggtcca gagcatcaga
    50041 ttcgctattt ctatttctgt cttttccgaa aggtctggtc gagtcaggaa tgggaaggtc
    50101 acatgcagaa actagagaga aaacaggaga ttgccaattt agaggaaatc tgcggtgcaa
    50161 gtttgtctgt ggggcagaaa ttggacttgg ttctctgggc tcacatcagt caacaacgtc
    50221 ttcgggtcaa tgcttgtcag tttcaagtca tagaagagaa aatgcgaggg tattttgaca
    50281 atattttcta ccttcgtttg ctgagaaagg ttccgtcctt ttttgctggg caacatattc
    50341 cactaggagt tgaggatggt gagatgatga tattcttctc ttttctccta tctcatcgca
    50401 ttcttcctct tcatactatg gagtatattc ttggttttgg agggcagttg gcagatttac
    50461 tgacgcaatt gattcaagaa atgaagaagg aggaactatt gggggattat acagaggacc
    50521 atgtcaccta tgaactcagt cagctttgtg ctcaagtcta tctctataag ggctatattt
    50581 tacaggatcg ctacaagtac cagttagaga atcgtcatcc atatttactg atggaacatg
    50641 attttaaaga gacagcagag gagatttttc atgctctacc tgcttttcaa caggggacag
    50701 atttagataa gaagattctc tgggaatggc tccagttaat cgaatatatg gctgaaaacg
    50761 gtggccagca tatgcggatt ggtctggatt tgacatctgg ttttcttgtc ttttcaagga
    50821 tggcagccat tttgaaacgg tatttggaat acaatcgttt tattaccatt gaagcttatg
    50881 accctagtcg gcattatgat ttgctggtta ccaataaccc gattcataag aaggaacaga
    50941 caccagtcta ttatttaaaa aacgacttgg atatggaaga tttggcaggg attcgtcagt
    51001 tattattcac ttaaaaggct tggttaatcc aggtcttttt tgtgaaattc acacaatctc
    51061 ctcacatttt tttaaaaatt aaaaaaagtt gataaacaag aaagcgcttt attttgtata
    51121 ctagtaagtg taaagaggaa acacctcaag atctttatca ggaggacagt acatgtcaca
    51181 agaaaaatac atcatggcca ttgaccaggg aactacaagt tctcgtgcca tcattttcaa
    51241 caaaaaaggg gaaaaggtta gctcgagtca aaaagagttt acccagattt tccctcaggc
    51301 aggttgggtt gagcacaatg ccaatgaaat ttggaactct gttcagtcag ttattgcggg
    51361 tgctttcatc gaaagtggtg tcaagccaaa tcaaatcgag gcaatcggga ttaccaacca
    51421 acgtgaaaca acggttgtct gggataagaa aacaggactt cctatctaca atgctatcgt
    51481 ttggcagtca cgccagacag cacctttggc tgagcaacta aaaagccaag gttatgtgga
    51541 aaaattccat gaaaagactg gtttgattat tgatgcttac ttctctgcta ccaaggttcg
    51601 ttggattttg gatcatgtag aaggtgctca agagcgagca gaaaaagggg aattgctctt
    51661 tggtactatc gatacttggt tggtttggaa attgactgac ggtgcggctc acgtgactga
    51721 ctactcaaat gcagctcgta ccatgcttta taacattaaa gaactcaaat gggatgatga
    51781 gattttggaa atccttaaca ttccgaaggc tatacttcca gaagttcgtt ctaactccga
    51841 aatctacggc aagacagctc cattccattt ctacggtgga gaggtgccaa tctcaggtat
    51901 ggctggggac caacaagcag ccctctttgg acagttggct tttgagccag gtatggttaa
    51961 gaatacttat ggaacaggct ctttcatcat catgaatact ggggaagaga tgcagttgtc
    52021 tgaaaacaac ctcttgacaa ccattggtta cggaatcaac ggtaaggttt attatgcctt
    52081 ggaaggttct atcttcatcg caggaagtgc tattcagtgg cttcgtgacg gtcttcgcat
    52141 ggttgaaaat tcaccagaat ctgaaaaata cgctcgtgat tctcacaaca acgatgaagt
    52201 ttatgtcgtt ccagccttta caggtctagg cgctccatac tggaaccaaa atgctcgtgg
    52261 ttccgtcttt ggtttgactc gtggaacaag caaagaagac tttatcaagg cgactttgca
    52321 atctattgct tatcaagtgc gtgacatcat cgacaccatg caagtggata ctcagaccgc
    52381 cattcaagta ctgaaggtgg atggtggtgc agccatgaac aacttcctca tgcagttcca
    52441 ggcggatatt ttaggcattg atattgcacg tgctaaaaac ttggaaacaa cagctctagg
    52501 agcggccttc ctagcaggtt tatcagtagg gtattggaaa gacttggacg agttgaaact
    52561 cttgaacgag acaggagaac tctttgagcc atctatgaac gaatctcgca aggaacaact
    52621 ctacaagggc tggaagaagg ctgtgaaagc aactcaagtc tttgcggaag tagacgacta
    52681 atactggcag aataaagcga tttatttaga aagtgtgtaa atatggaatt ttcaaagaaa
    52741 acacgtgaat tgtcaattaa aaaaatgcag gaacgcaccc tggacctctt gattatcggt
    52801 ggaggaatca caggagctgg tgtagccttg caggcggcag ctagcggtct tgagactggt
    52861 ttgattgaaa tgcaagactt tgcagaagga acatctagtc gttcaacaaa attggttcac
    52921 ggaggacttc gttacctcaa acaatttgac gtagaagtgg tctcagatac ggtttctgaa
    52981 cgtgcagtgg ttcaacaaat cgctccacac attccaaaac cagacccaat gctcttacca
    53041 gtttacgatg aagatggagc aacctttagc ctcttccgtc tcaaagtagc catggacttg
    53101 tacgaccttt tggcaggtgt tagcaacaca ccaactgcga acaaggtttt gagcaagaaa
    53161 gaagtcttgg aacgccagcc aaacttgaag aaggaaggct tggtaggagg tggagtgtat
    53221 cttgacttcc gtaacaacga tgcgcgtctc gtgattgaaa acatcaaacg tgccaaccaa
    53281 gacggtgccc tcattgccaa ccacgtgaag gcagaaggct tcctctttga cgaaagtggc
    53341 aagattacag gtgttgtagc tcgtgatctc ttgacagacc aagtatttga aatcaaggcc
    53401 cgtctggtta ttaatacaac aggtccttgg agtgataaag tacgtaattt gtctaataag
    53461 ggaacgcaat tctcacaaat gcgcccaact aagggagttc acttggtagt ggattcaagc
    53521 aaaatcaagg tttcacagcc agtttacttc gacacaggtt tgggtgacgg tcgtatggtc
    53581 tttgttctcc cacgtgaaaa caagacttac tttggtacaa ctgatacaga ctacacaggt
    53641 gatttggagc atccaaaagt aactcaagaa gatgtagatt atctacttgg cattgtcaac
    53701 aaccgctttc cagaatccaa catcaccatt gatgatatcg aaagcagctg ggcaggtctt
    53761 cgtccattga ttgcagggaa cagtgcctct gactataatg gtggaaataa cggtaccatc
    53821 agtgatgaaa gctttgacaa cttgattgcg actgttgaat cttatctctc caaagaaaaa
    53881 acacgtgaag atgttgagtc tgctgtcagc aagcttgaaa gtagcacatc tgagaaatat
    53941 ttggatccat ctgcagtttc tcgtgggtct agcttggacc gtgatgacaa tggtcttttg
    54001 actcttgctg gcggtaaaat cacagactac cgtaagatgg ctgaaggagc tatggagcgc
    54061 gtggttgaca tcctcaaagc agaatttgac cgtagcttta aattgatcaa ttctaaaact
    54121 taccctgttt caggtggaga attgaaccca gcaaatgtgg attcagaaat cgaagccttt
    54181 gcgcaacttg gagtatcacg tggtttggat agcaaggaag ctcactatct ggcaaatctt
    54241 tacggttcaa atgcaccgaa agtctttgca cttgctcaca gcttggaaca agcgccagga
    54301 ctcagcttgg cagatacctt gtcccttcac tatgcaatgc gcaacgagtt ggctcttagc
    54361 ccagttgact tccttcttcg tcgtaccaac catatgctct ttatgcgtga tagcttggat
    54421 agcatcgttg agccagtttt ggatgaaatg ggacgattct atgactggac agaagaagaa
    54481 aaagcaactt accgtgctga tgtcgaagca gctctcgcta acaacgattt agcagaatta
    54541 aaaaattaag aaaaaataaa agaggtggag ggcagcattc cttgtcgccc gtcccttctt
    54601 tttaatggag acagaaagat gatgaatgaa ttatttggag aatttctagg gactttaatc
    54661 ctgattcttc taggaaatgg tgttgttgca ggtgtggttc ttcctaaaac caagagcaat
    54721 agctcaggtt ggattgtgat tactatgggt tgggggattg cagttgcggt tgcagtcttt
    54781 gtatctggca agctcagtcc agctcattta aatccagctg tgacagttgg tgtagcttta
    54841 aaaggtgact tgccttgggc ttccgttttg ccttatatct tagcccagtt cgcaggggcc
    54901 atgctgggtc agattttggt ttggttgcaa ttcaaacctc actatgaggc agaagaaaat
    54961 gcaggcaata tcctggcaac cttcagtact ggaccagcca tcaaggatac tgtatcaaac
    55021 ttgattagcg aaatccttgg aacctttgtt ttggtgttga caatctttgc tttgggtctt
    55081 tacgattttc aggcaggtat cggaaccttt gcagtgggaa ctttgattgt cggtatcggt
    55141 ctatcactag gtgggacaac aggttatgcc ttgaacccag ctcgtgacct tggacctcgt
    55201 atcatgcaca gcatcttgcc aattccaaac aagggagacg gagactggtc ttacgcttgg
    55261 attcctgttg taggccctgt tatcggagca gccttggcag tgcttgtatt ctcacttttc
    55321 tagtttatac tcttcgaaaa tcaaattcaa accacgtcag cgtcgcctta ccgtactcaa
    55381 gtacagcttg cggctagctt cctagtttgc tctttgattt tcattgagta ttagaaaaca
    55441 attatgttga tagagcttgg gcaagagccc aatttcagca aaaaatgaag taaatcttct
    55501 cataataaaa cgcatcatat caagcacgaa aattccacga ggtcaactac agtcagaaag
    55561 ctgaacaaca agccaaaacg cccaaaaaag gcggcaaaaa gcaagcacct gcaagcaacg
    55621 tgccgaaatg gtcaaatcct gattatgtca acgaattaga cccaaaaatc gttgatatgc
    55681 tagtagaatt tcacaagtca caaggcactt tggaaactcc cgaggcgcaa gcagaaatcg
    55741 cccaaaaacg tgaagaaatc gagcaaagga gagctgagct tgagggtaaa aaacaagagc
    55801 ttttgaaccg cttgaacaaa tagagtttcg caagtattat gcttacaaat tacttgagca
    55861 attaactaaa atataaactc tgcctagata taaaggcagg gtttatattt tagaaattca
    55921 cgtaggttgt tacggttttt acatacccag tatagtttga gtttctatag tattcagtga
    55981 taaacttcca ttttctttga gcaacatgga tataagtact tgttatgtag tatggatatg
    56041 ggctttgtga atccaagtaa gactgataag cttgtatacc aaaatatgct ccaccaatta
    56101 ttgcacccca tggacccccc aataaagcac ctatcctacc aatcatataa ctgattccag
    56161 caccagtcat gaagttagcg aatgtgttag cttgtttatt cccatgtatt gtgttgacgt
    56221 aattccaaac attaggatcg tatgatctaa aagatatatt taggtcgatt tcattctttt
    56281 gataagccat ataaaatgcc ccattgatat agacgccgtc agcacgtcgt tcaatagtgt
    56341 ctacacttcc atctggattg acaacctcaa gaacttcatc gcttaaaata tttacttgcg
    56401 tatctccgaa ccgcactgat gagccattct caaactgagc ctcaccagat acaactttag
    56461 agtttgccga taagctatca tcagcaaaaa caacaagcga cggggaaatg ctagacatac
    56521 agaaaacaga cataactagc aaacacatgc atttaaacat cttagacata acggaaactc
    56581 ttttgtattt ttgatttttt tcaactttta ttatacaata aaaccaaata aaaagaaagc
    56641 ggtaacaata tgcttaatgc gaaaattttt tatatatttt tatgtttgat cgttatcgaa
    56701 actacaggct tgttgttgtt gaaaagaggt ctcgaaatgg gttatttaga cacagaagct
    56761 attatcctcg cagttttttc atttgctttt tacaacctat gttcattcgc ttgggtctgc
    56821 tctacaataa aaaacaataa aaaataaata gacgtatttt caaaaaaaaa aaaaaaaaat
    56881 gcatatttat attagcaaaa tgacgattta aatcgtcgtt tttttgt
//
LOCUS       DAVKMU010000006        56923 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKMU010000006 DAVKMU010000000
VERSION     DAVKMU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.006"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(99..632)
                     /gene="queF"
                     /locus_tag="VW343_000429"
     CDS             complement(99..632)
                     /gene="queF"
                     /locus_tag="VW343_000429"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9755041.1"
                     /translation="MKRQSALVVFSGGQDSKTCLFWTMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(939..1253)
                     /gene="trxA"
                     /locus_tag="VW343_000430"
     CDS             complement(939..1253)
                     /gene="trxA"
                     /locus_tag="VW343_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9755042.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(1270..1491)
                     /locus_tag="VW343_000431"
     CDS             complement(1270..1491)
                     /locus_tag="VW343_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9755043.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(1590..1970)
                     /locus_tag="VW343_000432"
     CDS             complement(1590..1970)
                     /locus_tag="VW343_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9755044.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(2115..3068)
                     /locus_tag="VW343_000433"
     CDS             complement(2115..3068)
                     /locus_tag="VW343_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9755045.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(3136..4344)
                     /gene="sstT"
                     /locus_tag="VW343_000434"
                     /pseudo
     CDS             complement(3136..4344)
                     /gene="sstT"
                     /locus_tag="VW343_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            4483..5043
                     /locus_tag="VW343_000435"
     CDS             4483..5043
                     /locus_tag="VW343_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9755046.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            5100..6008
                     /locus_tag="VW343_000436"
     CDS             5100..6008
                     /locus_tag="VW343_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9755047.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            5960..6550
                     /locus_tag="VW343_000437"
     CDS             5960..6550
                     /locus_tag="VW343_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9755048.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            6553..7659
                     /gene="yqeH"
                     /locus_tag="VW343_000438"
     CDS             6553..7659
                     /gene="yqeH"
                     /locus_tag="VW343_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9755049.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            7873..8184
                     /gene="yhbY"
                     /locus_tag="VW343_000439"
     CDS             7873..8184
                     /gene="yhbY"
                     /locus_tag="VW343_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9755050.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            8236..8865
                     /locus_tag="VW343_000440"
     CDS             8236..8865
                     /locus_tag="VW343_000440"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9755051.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVEYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            8865..9458
                     /gene="yqeK"
                     /locus_tag="VW343_000441"
     CDS             8865..9458
                     /gene="yqeK"
                     /locus_tag="VW343_000441"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9755052.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVGKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            9459..9959
                     /locus_tag="VW343_000442"
     CDS             9459..9959
                     /locus_tag="VW343_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9755053.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEEHFADVL
                     DYKHIF"
     gene            9972..10325
                     /gene="rsfS"
                     /locus_tag="VW343_000443"
     CDS             9972..10325
                     /gene="rsfS"
                     /locus_tag="VW343_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9755054.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            10428..11174
                     /locus_tag="VW343_000444"
     CDS             10428..11174
                     /locus_tag="VW343_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9755055.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKEKKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            11184..12281
                     /locus_tag="VW343_000445"
     CDS             11184..12281
                     /locus_tag="VW343_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9755056.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVNILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTARSVEELVELVTTKRY
                     TKARVRRLLTYILMQARENVLPEVIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNPSIADQNFGRVPIRIETN"
     gene            12519..12773
                     /locus_tag="VW343_000446"
     CDS             12519..12773
                     /locus_tag="VW343_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9755057.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            12777..13031
                     /locus_tag="VW343_000447"
     CDS             12777..13031
                     /locus_tag="VW343_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9755058.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            13222..14835
                     /locus_tag="VW343_000448"
     CDS             13222..14835
                     /locus_tag="VW343_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9755059.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            14965..15591
                     /gene="gmk"
                     /locus_tag="VW343_000449"
     CDS             14965..15591
                     /gene="gmk"
                     /locus_tag="VW343_000449"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9755060.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            15616..15930
                     /locus_tag="VW343_000450"
     CDS             15616..15930
                     /locus_tag="VW343_000450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9755061.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            15996..18392
                     /locus_tag="VW343_000451"
     CDS             15996..18392
                     /locus_tag="VW343_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9755062.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQVRILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTRNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            18405..19340
                     /locus_tag="VW343_000452"
     CDS             18405..19340
                     /locus_tag="VW343_000452"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9755063.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPNRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            19333..20646
                     /gene="rsmB"
                     /locus_tag="VW343_000453"
     CDS             19333..20646
                     /gene="rsmB"
                     /locus_tag="VW343_000453"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9755064.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVALKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFANGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            20661..21401
                     /locus_tag="VW343_000454"
     CDS             20661..21401
                     /locus_tag="VW343_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9755065.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            21398..23377
                     /gene="pknB"
                     /locus_tag="VW343_000455"
     CDS             21398..23377
                     /gene="pknB"
                     /locus_tag="VW343_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9755066.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            23492..24250
                     /locus_tag="VW343_000456"
     CDS             23492..24250
                     /locus_tag="VW343_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9755067.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            24252..24701
                     /locus_tag="VW343_000457"
     CDS             24252..24701
                     /locus_tag="VW343_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9755068.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            24763..25389
                     /locus_tag="VW343_000458"
                     /pseudo
     CDS             24763..25389
                     /locus_tag="VW343_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            25457..25705
                     /locus_tag="VW343_000459"
     CDS             25457..25705
                     /locus_tag="VW343_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755069.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            25836..27008
                     /locus_tag="VW343_000460"
     CDS             25836..27008
                     /locus_tag="VW343_000460"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9755070.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTVLSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            27008..28282
                     /locus_tag="VW343_000461"
     CDS             27008..28282
                     /locus_tag="VW343_000461"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9755071.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQDDPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(28318..29283)
                     /locus_tag="VW343_000462"
     CDS             complement(28318..29283)
                     /locus_tag="VW343_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9755072.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVTTTGYFLPVTLLPGKSI"
     gene            complement(29264..30718)
                     /locus_tag="VW343_000463"
     CDS             complement(29264..30718)
                     /locus_tag="VW343_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9755073.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            30771..30968
                     /locus_tag="VW343_000464"
     CDS             30771..30968
                     /locus_tag="VW343_000464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755074.1"
                     /translation="MQAFYVKKEEKLSKDYHKINTGNQSNFYENVKDNEIKDFLTKVS
                     NLFFKEFLMKQSKTINLKLAY"
     gene            31094..32977
                     /locus_tag="VW343_000465"
     CDS             31094..32977
                     /locus_tag="VW343_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9755075.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFIIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGDKVKVGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            33126..34013
                     /locus_tag="VW343_000466"
     CDS             33126..34013
                     /locus_tag="VW343_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9755076.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(34056..34673)
                     /locus_tag="VW343_000467"
     CDS             complement(34056..34673)
                     /locus_tag="VW343_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9755077.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(35088..35276)
                     /locus_tag="VW343_000468"
                     /pseudo
     CDS             complement(35088..35276)
                     /locus_tag="VW343_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(35786..35866)
                     /locus_tag="VW343_000469"
                     /pseudo
     CDS             complement(35786..35866)
                     /locus_tag="VW343_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            35982..36875
                     /locus_tag="VW343_000470"
     CDS             35982..36875
                     /locus_tag="VW343_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755078.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            36872..38071
                     /locus_tag="VW343_000471"
     CDS             36872..38071
                     /locus_tag="VW343_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755079.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(38424..39191)
                     /locus_tag="VW343_000472"
     CDS             complement(38424..39191)
                     /locus_tag="VW343_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755080.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(39208..39903)
                     /locus_tag="VW343_000473"
     CDS             complement(39208..39903)
                     /locus_tag="VW343_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755081.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(39908..40273)
                     /locus_tag="VW343_000474"
     CDS             complement(39908..40273)
                     /locus_tag="VW343_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9755082.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            40413..40886
                     /gene="nrdR"
                     /locus_tag="VW343_000475"
     CDS             40413..40886
                     /gene="nrdR"
                     /locus_tag="VW343_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9755083.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            40887..42056
                     /locus_tag="VW343_000476"
     CDS             40887..42056
                     /locus_tag="VW343_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9755084.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            42057..42953
                     /gene="dnaI"
                     /locus_tag="VW343_000477"
     CDS             42057..42953
                     /gene="dnaI"
                     /locus_tag="VW343_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9755085.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            42950..43663
                     /locus_tag="VW343_000478"
     CDS             42950..43663
                     /locus_tag="VW343_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9755086.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAKMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            43677..44987
                     /gene="der"
                     /locus_tag="VW343_000479"
     CDS             43677..44987
                     /gene="der"
                     /locus_tag="VW343_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9755087.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            45078..45413
                     /locus_tag="VW343_000480"
     CDS             45078..45413
                     /locus_tag="VW343_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755088.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            45553..45888
                     /locus_tag="VW343_000481"
     CDS             45553..45888
                     /locus_tag="VW343_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755089.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            46073..46864
                     /locus_tag="VW343_000482"
     CDS             46073..46864
                     /locus_tag="VW343_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755090.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILHVVPYMVLKKYEDNV"
     gene            46857..47498
                     /locus_tag="VW343_000483"
     CDS             46857..47498
                     /locus_tag="VW343_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755091.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            47502..48152
                     /locus_tag="VW343_000484"
     CDS             47502..48152
                     /locus_tag="VW343_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9755092.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            48163..48450
                     /locus_tag="VW343_000485"
     CDS             48163..48450
                     /locus_tag="VW343_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755093.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            48596..51109
                     /gene="secA"
                     /locus_tag="VW343_000486"
     CDS             48596..51109
                     /gene="secA"
                     /locus_tag="VW343_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9755094.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            51190..52221
                     /locus_tag="VW343_000487"
     CDS             51190..52221
                     /locus_tag="VW343_000487"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9755095.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            52223..53254
                     /locus_tag="VW343_000488"
     CDS             52223..53254
                     /locus_tag="VW343_000488"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9755096.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            53293..53661
                     /locus_tag="VW343_000489"
     CDS             53293..53661
                     /locus_tag="VW343_000489"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9755097.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            53651..54754
                     /locus_tag="VW343_000490"
     CDS             53651..54754
                     /locus_tag="VW343_000490"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9755098.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            54773..56788
                     /gene="recG"
                     /locus_tag="VW343_000491"
     CDS             54773..56788
                     /gene="recG"
                     /locus_tag="VW343_000491"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9755099.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHYMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIETWQEDPEWRMIALHLEKKEHLD"
BASE COUNT        16827 a        10959 c        12989 g        16148 t
ORIGIN      
        1 agctgacttc gtcagtttta tctacaacct caaacactgc tttgagtaac ctgcggctaa
       61 cttcctagtt tgctctttga ttttcattga gtatgacttt agcggttgtc aattttctct
      121 ggataaaggt cgtgttggaa gaggcgttgt tctgccaagc cctcatactt agttccttgc
      181 ttaccgtagt tatagtaggg gtcgattgaa atgccaccgc gcggagtgaa ttttccccag
      241 acttctaaat agcgagggtc tagcaagttg accaagtctt tcccgatggt gttgatacag
      301 ttttcgtgga aatctccgtg gtttcggtag ctaaatagat atagtttgag ggattttgac
      361 tcgacacaga gcttgtcagg aatgtaggaa atatgaatcg tcgcaaagtc tggctgagca
      421 gtgatttgtc ccagcagaga catatcgagg atatggtgac gaatgccctg ttccttagcg
      481 atttctctag taatttgaat ttcgaggtga tgacgttggc cgtaggcaaa ggtgacagct
      541 tcgactgttt catagtgttg catggtccag aaaaggcagg tttttgaatc ttgaccacca
      601 ctaaagacga ccaaggctga ttgacgtttc atagtactcc ttccaaaatg ggaaatgttc
      661 agagcacgta aaaagctccc attagggagc taaaaaatac caaatcgagg ttttttttag
      721 cgatggcata tcccaaatat cgtaatattc tatttatata gtaaaatgaa ataagaacag
      781 gacaaatcga tcaggacagt caaatcgatt tctaacaatg ttttagaagt agagatgtac
      841 tattctagtt tcaatctact atagtctagc atatcttttg aaaaatggca aagggcaaga
      901 aaaaagagac caaagaaagt acttggtctc tcgtttgatt agctcaattc agcaatgatg
      961 gccttgattt gttctgctgt gtgaacacct gcaacttgtt tgacaacttg gccgtctttt
     1021 ttgaagagaa gagttggaat agacatgatt ccaaaagcac gagctgtgtt tggattttca
     1081 tcaacgtcca ttttaacgat tttcaagaca tcttctgaaa gttcttcaga caatttgtcc
     1141 aagattggac cttgcatacg acatggacca caccaagttg cccagaagtc tactaagacc
     1201 aaaccgtctt ttgtttcttg ttcgaatgtt gcatctgtaa ttgcttttgc cattgtattt
     1261 ctcctttttt tagttatatt ggcttaaatc ttgtttcatg agatagaaga agatatctcc
     1321 ataagtccca tggtagtcca aattatgacc cttgtaagtt aatttttgga cagggtagta
     1381 gtctgcgacg ccgataaggc aagcttgttg cgaacgttca aagtcttcat aagactcgaa
     1441 agtcacagtt ctttcgttct tgctggcatc tatataggta atttcaatca tgtttaaaac
     1501 tcctttgttt aatgctaact ttattttact ccttataaaa gagaatgtca agaaaaatga
     1561 ttgcgcacgc aacttttttt aaaatcatct taaatcaaga aatccaaacc tgcttccaag
     1621 ctttcttcga cagtcttttg tagcgaggcc agtgtctttt gcccatcatt tgtcaggcag
     1681 ataaaactag agcgtctatc ttgatggcaa cacatgcgac tgagtagacc gcaattttta
     1741 gcttccaagc gagccaccat cctagaaact gcgctcgggc tcagatgaag cttatctggc
     1801 aggtcaatct ggcgtagaga tttttcttca gccaagtcca gatagtagag caggtagaac
     1861 tctttcaagg tcagactttg ctcgctctgt tgggcaatgg tctcttccaa gagactttca
     1921 atttctttct gacgccgatt gaagtcaaac catttttcca aataggtcat agtgtctcct
     1981 ttctttttag agtcataatt atagaagaaa gtccattaac gggcagtctt tgcgtcataa
     2041 gatgattgcg catgcaataa ttatactact tttcaagaat gctggcaagc tctgtttttt
     2101 agtggttttc ttttttactg tctatatttt tggtaaaaat caacttttac ttggatgaag
     2161 gttttggctt cacgtaggag ttgaagaaga gtggcgcggg tttcaaactc ttctcttgtc
     2221 ttgggcagac tgcggttccg gaagacttcc agataacgtt caatttcatc tagcaaatca
     2281 gaagcaggat tggtctggct cagttgacct gcaatttttg aaaagagttg cgctaagatc
     2341 aggctttcac tggcggcaag gtgacaagtg ttaatctgtt gggccatgtt tctcaggata
     2401 cgactttgtc gttgtctcat ctcaaagtag tggatatggt agtcggtctg gtgaaagagg
     2461 tggtcagagt gatccaaata gaccagtctg agggcttctt tcaaaagcgt gtctaattct
     2521 gctaccagct gtgctcggtt gcgtccgtct cctctggata aatagtattt gaagcgctgg
     2581 aggatatctt ttaacttttc ttccaccagc gtgtggtagt gctggatttc ctcttctcgt
     2641 gaaggcatat agagattaac aagcaaggca aatcctgtac caatagcaaa gagaaggaat
     2701 tcgttgacta ggaggtctgg agaggttgac tcttgaacca agagatggct aaccaaaacc
     2761 gtgcttggtg tgatgccaat ttcccagccc atcttgtagg ctaaaggaac gtagaaggcc
     2821 agatagaggc cgagactcca gatatgaaat ccgctcaagt gaaaagctag aacgccgata
     2881 gccagagcta gaagcataga aaaaagacga ttgcgagcca gttttaaagt acttctacgc
     2941 gtatcagata ggctcaagag agcgataatt ccagccgaaa ctgctgacga aagattgaga
     3001 aaataagcaa gcaggcaggc aagacaggta gctaagatga gcttggtcgt acgttggcta
     3061 atagacataa gaatttccta ataagttaga ataaaagcgt aaaagacaag acatgagcag
     3121 gcttgccttg atgagttatt ttttacgggt tgctgcgtat tcggcaacgg cggtaaagag
     3181 gacatctgta gaagagttaa gggctgtttc acatgagtct tggatgacac caatcacaaa
     3241 accaacccca acaatttgta tggcaatatc gttagaaata ccgaaaaggc tacaagcaac
     3301 tgggataaga aggagggaac ctccggcaat acctgaagca tcacaggctg agatagctgc
     3361 taccacactg aggacaaagg ctgtggcaaa gtcaacagga attccaagag tgttaactgt
     3421 aacaagggtc aaaacgttaa tggtaatcgc tactccagcc atattgatag tagaaccgag
     3481 tgggatagaa acagaatagg tatctgggtt gagtccaagg tcatggcaga gtttcatgtt
     3541 gacaggaatg ttagtcgcag aactacgagt gaaaaaggct gtcacaccgc tgacacggag
     3601 gcagttccaa actagagggt aaggattgcg tctcataaag aagaaggcaa tcaaagggtt
     3661 gaccacaggg gcaacaaaaa gcatagtcgt tactaataga accaataaaa taccgtagtt
     3721 ggcaaggctt ccgactccct tgtcagaaat ggttttaaaa acaagaccaa ggattccaaa
     3781 tggagccaga ttgatgatcc attcgacaat tttagaagtc acgtcagcga tagtttttag
     3841 caattcttta ctatttttac tggcttctct catagcgatt ccaaaaatga ctgcccaaga
     3901 taagattcta atatagttag cagtaagcag ggcgttgact gggttgtcaa ccagtttgag
     3961 caagaggttg ctgaggacct gcccaatccc atctggtggt gcaatttcag tattggcact
     4021 atttagggta atttcaatag ggacgatgaa acttgctagt acagctacaa gagcagcggc
     4081 gaaagtccct atcataggat atacaagaaa acaacagttt tcatattgct atcttgtccc
     4141 ttttgatgtt ggaaagggca ttggcaacga gggcaaagac taggatagga gcaacagctt
     4201 ttagacctcc aacgaataaa tccccgagta gcccaatccc tgagagatta ggaagggtca
     4261 gtcctaggat tccccacaaa gcataccaat caagatacgc ttgacaaggc ttgccttatt
     4321 ccaagcatga atgattcttt tcataataat ctcctttttg tgtagtgatt atgattatag
     4381 tataaatgat agacaaaatc aagaattttc tgtctatttt ttgaatattt atggagaatg
     4441 agactgatga aaatatggta taatgaaata aaggagtttt atatgcaaaa atttattcag
     4501 gcttatattg aaaagctaga tgtgacaacc attatcgaga atattctaac caaggtcatt
     4561 tctcttttac tgcttttgat tgtattttat attgctaaaa aaatgcttca taccatggtg
     4621 cagagaattg tcaaaccttc tctaaaaatg tctcgtcatg atgtcggacg ccaaaaaacc
     4681 atctcacgtt tactagaaaa tgtgtttaat tatacgctat atttcttttt actctactgc
     4741 attttgtcga ttttaggttt gccagtttct agtttgctgg ctggagctgg tattgctggg
     4801 gtagcgattg gtatgggagc ccaaggcttt ctgtctgatg tcatcaatgg ctttttcatc
     4861 ctctttgaac gtcaactgga tgtgggagat gaggtcgttc tgacaaatgg accgattact
     4921 gtatcgggta aggttgtcag tgtgggaatt cgtacgacac agcttcgtag cgaggagcaa
     4981 gcccttcact ttgtccctaa ccgaaatatc acagttgtta gcaatttctc acgcacagac
     5041 tagacctgtt attttaagta atttgtggta caatagaggg agtttaataa ggagaaaaga
     5101 tggttttaga aaagcagttg ggcaatggtt gtacctggat agacctagac ctaggaaagt
     5161 tgaataaact agaagacctt tctgaaattt acggtttgga caaggaaacc attgaatacg
     5221 cactggatag aaacgagcgc gcccacatgg attaccaccg tgaaagtggg acggttacct
     5281 ttatctataa tgtcctagac gtaaaaaagg acaaggccta ctatgagact tttcccatga
     5341 cctttattgt cgagcatcgt cgcctgatta ccattagtaa taccaagaac gcctatgtca
     5401 ttgaacagat gactcgttat ctggagagcc atgacacgct ttcgatttat aagtttctct
     5461 ttgccagtct ggaaattatc agcaatgcct actatcctgt cattgagcag atggacaaga
     5521 gtagggatga ggtcaatgac ctcttgcgcc agcgaactac caagaaaaac ctctttgccc
     5581 tgtctgattt ggagactggt atggtttatc tgacggcagc tgccaaacaa aatcggattt
     5641 tgttagagca tattcaaggt catgccttgt atcgtagttt tgatgagatt gagagagaac
     5701 agtttgatga tgccatgatt gaggctcatc agctggtatc catgacagac ctaatctctc
     5761 agattttaca gcagctttca gcctcttaca acaatattct aaacaataat ctgaatgaca
     5821 atttgacaac cttgactatc atttcagtct tgctagctgt tttggcagtc gtgacaggct
     5881 ttttcggaat gaatgttccc ttacctttaa cagatgagcc ccatgcttgg ctctatatca
     5941 gtttggctag tgcaggtttg tggattgttt tatccttgtt actaaggaaa attgcgaaaa
     6001 aaagttaaga aaaggagcca gaatggcgat tgaaaattat ataccagatt ttgctgtgga
     6061 agcagtctat gatctgacag tcccaagcct gcaggcgcag ggaatcaagg ctgttttggt
     6121 cgatttggat aataccctca ttgcttggaa caaccctgat ggaacgccag agatgaagca
     6181 atggctacat gaccttcggg acgcgggtat tggcattatc gtagtgtcaa ataacaccaa
     6241 aaaacgcgtt caacgagcag ttgagaaatt tgggattgat tacgtttact gggccttgaa
     6301 gcccttcaca tttggtattg accgtgctat gaaggaattc cactatgaca aaaaggaagt
     6361 ggtcatggtt ggcgaccagc tcatgacaga tatacgagca gcccaccgtg cagggattcg
     6421 gtcaatttta gtcaaaccct tggtccaaca tgactcaatc aaaacgcaga ttaaccgaac
     6481 tcgtgagcgt cgtgttatga gaaaaatcac tgaaaagtac ggaccgatta catataaaaa
     6541 aggaatttaa ctatggaaga aattctctgt attggttgtg gagcaaccat tcagacgaca
     6601 gataaagctg gtcttggttt taccccccag tcggcacttg aaaaaggttt ggagactggc
     6661 gaagtctatt gccaacgctg tttccgtctc cgccactaca atgaaatcac agatgtccag
     6721 ttgacgaacg atgatttcct caagctcttg cacgaggtgg gagacagtga tgccttagtg
     6781 gtcaatgtca ttgatatctt tgattttaat ggatctgtca tcccaggttt accacgtttc
     6841 gtctcgggca atgatgtcct cttggtagga aataaaaaag atatccttcc taagtcagtt
     6901 aagtctggta agattagcca gtggctcatg aaacgtgccc atgaagaagg tcttcgtcca
     6961 gtcgatgtgg tcctaacttc agcacaaaat aaacatgcca ttaaggaagt cattgacaag
     7021 attgaacact accgtaaggg ccgcgatgtc tatgtggtcg gtgtgaccaa cgttggaaaa
     7081 tcaactctaa tcaatgctat tatccaagaa atcacgggtg atcagaatgt catcactact
     7141 tcacgcttcc caggtacaac cttggacaaa atagagattc cgcttgacga cggatcttat
     7201 atttacgata cgccgggaat tatccaccgc caccagatgg ctcactactt gacggccaaa
     7261 aacctcaagt atgtcagtcc taaaaaggaa atcaagccta agacctatca gcttaatcct
     7321 gagcaaaccc tatttttagg tggtttggga cgctttgact ttatagcagg agaaaagcaa
     7381 ggatttactg ctttctttga taatgaactc aaactccatc gtagcaagct tgaaggagct
     7441 agtgctttct acgataagca cctgggaact cttctgacac caccaaatag caaggaaaaa
     7501 gaagatttcc caaggctagt ccagcatgtc tttaccatta aagataagac agacctagtc
     7561 atctcaggcc taggctggat tcgtgtaaca ggcatagcaa aagtcgccgt ctgggcacca
     7621 gaaggcgtcg ccgtcgtcac acgaaaagca attatttaaa acaaaatcgc tgagcgaaca
     7681 aagtgagctc atatagcgat agttattgcc gtggtgtgat tgccaatctg tagattggca
     7741 aggggcacaa ttgagaccaa ggctcaattg tgaggtcagg aaatccattt ttcaaagatg
     7801 aaatctttgg aaaattagtt ccgaccgtcc ctcaccacct aaaataacta tccaaaaaca
     7861 aggaagaaaa ttatgtcatt aacatcaaaa caacgtgcct tcctcaacag ccaggcacac
     7921 accctcaaac ctatcatcca aatcgggaaa aatggactca acgaccaaat caaaaccagc
     7981 gtccgtcaag ctcttgatgc gcgtgaatta atcaaggtta ctctcttaca aaacacagat
     8041 gaaaacatcc acgaagtagc tgaaattttg gaagaagaaa tcggtgtgga tacagtccaa
     8101 aaaataggac gcatcttgat tttgtttaaa caatctagca agaaagaaaa tcgcaagatt
     8161 tctaagaaag tcaaagaaat ctaagaccga aactccaaat aactgttttt atagagaaat
     8221 aaaggggact agcctatgac aatcgaacta ttgactccct ttaccaaggt agagttggag
     8281 ccagaaatca aggagaaaaa acgcaaacaa gttgggattt taggggggaa ttttaaccct
     8341 gttcacaatg cccatctcat tgttgcggat caagtacggc aacagttggg actggatcaa
     8401 gttcttctca tgcctgaata ccaacctcct cacgttgata aaaaggaaac catccctgaa
     8461 caccatcgtc tcaagatgct tgagttggca attgagggaa ttgacggcct agtcattgaa
     8521 accattgagt tggagcgcaa gggtatttcc tacacctacg acaccatgaa gattttgaca
     8581 gagaagaatc cagatacgga ttattacttt atcatcggtg ccgacatggt tgagtatctg
     8641 cctaagtggt accgaattga tgaactggtt gacatggttc agtttgtggg ggttcagcgt
     8701 ccacgctaca aggtagggac ttcctatcca gttatctggg tggacgtacc gctcatggat
     8761 atctcgtcca gcatggtgcg ggacttcctt gcccaaggtc ggaaacccaa ctttctccta
     8821 cctcagccag tgctagacta catcgagaag gaggggctct actgatgtcc tatcaagact
     8881 atatcaactg ctcccgtgag gccttgttgg aaaaaatggc agagcttcta cctgagaaac
     8941 gtctaaccca ttgtctaggt gtggagcgtg ccgctatgga attagctcag cgctttggtg
     9001 tagatgttgg gaaagcaagt ctagcaggtc ttcttcatga ctatgctaaa aagttgtcaa
     9061 atcaggaatt tctagccttg attgaccgtt accagctaga ccctgacctc aaaaactggg
     9121 gcaataatgt ctggcatggt atggtcggaa tttacaaaat tcaggaagat ttggatttgc
     9181 atgattctga aattctgcga gccattgaaa ttcatacagt cggagctggt caaatgacag
     9241 acctagataa ggtcatctac gtcgcagact atatcgagca caatcgagcc tttcctggag
     9301 tagatgtggc gcgtgagatt gcaagtctat cgctcaataa ggcggtggcc tacgaaacag
     9361 ctcgtaccgt ggagtatcta gctcatcagg gattccccat ctatccccaa acccttgaaa
     9421 cctataacgc ctttgtgcac tatttgaaag aggactaaat gaagacggct tttataatca
     9481 ttgatgttca gaatgtttta gtagaaactg ggttccagac aaaaagtcta ttggaaaaaa
     9541 tttcttattt acaaaaccag gctagaagca agaatattga aattatctat gttcaacata
     9601 ttgagaattc tgaagctcaa acatcagaag attggcagtt atctgcgctt ttaaatcgaa
     9661 aacctgctga aaaggtcttt cagaagaagt ataacagtat tttcaaagaa actggcttaa
     9721 aagaatactt ggataaacag gggattgaaa aattagtttt atgtggtatg cagacagaat
     9781 attgtgtgga tacctctgtc aaggttgcct ttgaatatgg ctatcagctt attgttccag
     9841 aaggtgctgt cacaaccttt gatggggatg acattccagc agaaacgatt aatgaatttt
     9901 atgaggacat ttgggaggag cactttgcag atgtcctaga ttacaaacat attttctaaa
     9961 aagaggatta aatgaacgaa aaagaattac tagaactagt cgtgaaagcg gctgatgaga
    10021 aacgtgcgga ggatatcctc gcacttgatg tacaagattt gactagtgtg acggactact
    10081 ttgtcatcac tagctcaatg aatagccgtc agttggacgc tatcgcagct aatatccgtg
    10141 aaaaagtagc tcaagcgggc tttaaaggta gccatgtcga aggcgatgca gctggaggct
    10201 gggtcttact ggacctcggt gctgttgtcg tgcatatctt ttcagaagaa atgcgtgccc
    10261 attataatct agagaagcta tggcatgagg cgaattcagt agatatttca gaagctcttg
    10321 cttagaagat gaactcagtt gtagtcaact gggtttcttt gcttatttta gaaaaattga
    10381 tagaaaggta aagggctagt ggtgtttacc atggagctct tagtatcatg attatggcga
    10441 cttatgaaac ctttgcggcg gtctatgatg cggtcatgga cgatagttta tacgacaaat
    10501 ggacgaattt ttctctgcgt catttgccta agaccaagga gaaaaagaaa ctcttggaac
    10561 tggcttgtgg gacaggaatt caatcagtgc gcttctctca ggctggtttt gatgtgactg
    10621 gacttgactt gagtgcggat atgttgaaga ttgcggagaa gagagcaact tcagccaagc
    10681 aaaagattgc ttttatagaa ggtaacatgc tgaatttgtc caaggcaggg aaatacgatt
    10741 ttgtcacgtg ttattcggac tctatctgct atatgcagga tgaggtggaa gtaggggacg
    10801 tctttaagga cgtgtacaat gcgctgaatg aagagggagt tttcatcttt gacgtgcact
    10861 cgacctacca gacagatgaa gtattccctg gctattccta ccatgaaaat gcggaagatt
    10921 ttgccatgct ttgggataca tatgaggacg aagctcctca ctccatcgtg catgagctga
    10981 ctttctttat caaggaggct gacggttcct ttagtcgcca cgatgaagta catgaggaga
    11041 ggacctatga gatcttgacc tatgatatct tactggaaca ggctggattc aagtccttca
    11101 aactctatgc agactttgag gataaggaac caacagaaac tagcacccgt tggttttttg
    11161 tggcgcagaa gtaggagatt agcatgacca tcacaggtat tatcgcggag ttcaatcctt
    11221 ttcataatgg ccataaatac ctgctggatc aggcagaggg actgaaaatt gttgccatgt
    11281 cagggaattt catgcagcgt ggagagcctg ctattgtgga taagtggaca agggcccaga
    11341 tggcgctgga aaatggcgca gacttggtag tggaattgcc ctttttagtc agtgttcagg
    11401 cagctgattt ctttggtcaa ggagctgtga atatcttgga tcgcttgggc attgatagtc
    11461 tagtttttgg gacagaggaa gttcgagatt atcagaaaat cgctgactta tacacagaga
    11521 aaggtgctga gatggagaaa tttgtggaaa atctgcctga ttccctttcc tatccccaga
    11581 aaacccaagc tatgtggaag gaatttgctg gtcttgattt ttcaggtaat acccccaatc
    11641 atgttcttgc tctggcctat gccaaggcgg ttgcaggacg aaatatcaag ctgcatccga
    11701 ttcagcgtca gggggcaggt taccattctg tgaataaaga tgtggacttt gcctcggcga
    11761 cagccctccg tcaacatcag aaggaccaag atttcttaga acgctttatg ccttctgttg
    11821 ccctatttga gcaggccagt aaggtgatct gggaagacta ttttcccttg ctccgctatc
    11881 aaatcttgtc aaatcccgac ctaaccacca tctatcaggt caatcaagaa atggctgtgc
    11941 gcattaaaga agctattaaa acagcccggt ctgtggaaga attggtcgag ttagtgacta
    12001 ccaaacgtta caccaaggcg cgtgtcagac gcctattgac ctatattttg atgcaggcta
    12061 gagaaaatgt cttgccagaa gtcattcatg tccttggatt tactgaaaaa ggtaggcaac
    12121 atctcaagtc tttgaaaggt caggtcagtc tagtcagccg aattggcaaa gaaccttggg
    12181 atgctatgac ccaaaaagca gaccagattt accaactagg aaatccaagt atagcagacc
    12241 agaattttgg aagagtgccg attagaatag aaacaaacta agtctaccga aaggtaggct
    12301 ttttgagatt ttttacgaat aatactcttc gaaaatcaaa ttcaaatcgc gtcaacgtcg
    12361 ccttgccgta ctcaagtaca gcctgcggct agtttcctag tttgctcttt gattttcatt
    12421 gagtataaat agatagaaaa gaaaatcttg tacaagttcc tgacaatttc tttcttttcg
    12481 tgtataatag tggaaaagag ctaaaacgag gtatggttat ggaagcagtc ctttactcaa
    12541 cattccgaaa tcatttaaag gactacatga agaaggtaaa tgatgaattt gagcctttga
    12601 cggtggtcaa taaaaatcca gatgaggaca ttgtagtcct ttcaaagagt gagtgggata
    12661 gtatccaaga aaccctgaga attgctcaaa ataaggaact ttctgataag gttttgcgag
    12721 gaatggctca agttcgtgct ggaagtactc aggtccatgt tattgaggag taagaaatgc
    12781 tgctcaagtt tacagaagat gcctgggcag attattgcta ctggcaaaat caggataaga
    12841 aaacgttaaa aagaatcaat aaactaatca aggatattca acgtgatccc tttacaggaa
    12901 taggtaaacc agaaccactc aaatatgatt accaaggagc ctggtcacgg cgtattgatg
    12961 cagaaaatcg cttgatttat atgatggatg gagatagcgt ggctttcttg tcctttaaag
    13021 atcattacta agtctattga aaggtaggct ttttctcaag tctctactaa actactattt
    13081 ctaaacaaca agtttatttt tgcaaatgtg ttccaaattc cccaaaacat attctatttt
    13141 aggtttgtga aaatcacttt tttatggtag aatgtaaaag aatgtatgtc attcggaata
    13201 acaataaaat gaggtaaaaa catggaaatc atgtcgcttg cgattgctgt ttttgccgtc
    13261 atcattggtt tagtcattgg atatgtcagc atctcagcta agatgaaatc atctcaggaa
    13321 gctgcagagt tgatgctttt aaatgctgaa caagaagcaa ctaatttacg tggacaagct
    13381 gagcgtgaag cggatttact tgttaatgaa gccaaacgtg aaagcaagtc tcttaaaaaa
    13441 gaagcactat tggaggccaa agaagaagcc agaaaatacc gtgaagaagt ggacgctgaa
    13501 ttcaaatcag aacgtcaaga actcaaacaa atcgaaagtc gtttgacaga gagagctact
    13561 agccttgacc gtaaggacga caatttgacg agtaaagaac aaacacttga acaaaaagaa
    13621 caaagtattt ctgatagagc gaaaaacctt gatgcgcgtg aagagcaatt agaggaagtc
    13681 gaaagacaaa aagaagcaga actagagcgt attggtgctc tgtctcaggc agaagcacga
    13741 gatattatct tggctcagac agaggaaaac ttgaccaggg agattgccag tcgcattcgc
    13801 gaagctgagc aagaggtcaa ggaacgttct gacaaaatgg ccaaggacat cttggttcaa
    13861 gctatgcaac gtatcgctgg tgaatatgta gcggagtcaa caaactcaac ggttcatctg
    13921 ccagacgata ctatgaaggg acgcattatt ggtcgtgaag gtcgtaacat tcgtaccttt
    13981 gaaagtttga caggggtcga tgtgattatc gacgatacac cagaagtggt gaccttgtca
    14041 ggatttgatc cgattcgtcg tgagattgcc cgtatgacta tggaaatgtt gctcaaagat
    14101 ggtcgtatac atccagctcg tatcgaagag ttggttgaga aaaaccgtca agagattgac
    14161 aataagattc gtgaatacgg tgaggctgct gcctatgaaa ttggtgcgcc aaaccttcat
    14221 ccagacttga tgaagattat gggacgtttg cagttccgta cttcatatgg acaaaatgtt
    14281 ttgcgccatt cgattgaggt tgctaagttg gctggtatca tggcgagcga acttggtgaa
    14341 aatgcggctc ttgcccgtcg tgctggattc cttcacgata tcgggaaagc cattgaccat
    14401 gaggttgaag gtagccacgt tgaaatcggt atggaattgg cccgtaagta caaggaaccc
    14461 ccagttgtgg tgaatacgat tgctagtcac cacggagatg ttgaagctga gagcgtgata
    14521 gcagttatcg tcgctgcagc agatgccttg agcgcagccc gtccaggtgc tcgtagtgag
    14581 tctcttgaaa gctacatcaa gcgtctccat gatttggaag aaattgctaa cggctttgaa
    14641 ggagtgcaaa ctagctttgc ccttcaagca ggacgtgaaa ttcgtatcat ggtcaatcca
    14701 ggaaaaatca aggacgacaa agtcacaatc ttggctcaca aagttcgtaa gaaaattgaa
    14761 aacaatctcg attatccagg aaatatcaag gtaaccgtga ttcgcgagct tcgtgcagta
    14821 gattatgcta aataaataag aaagagcagt tgaaaaatta ctgctttttt gttacactag
    14881 atagaaagac tgtaaaggat aatctggctt tttgccatta ttctagagaa atgttatttc
    14941 acagattgac taaaaggaga cacaatggca gaccgaggct tactaatcgt tttttctggt
    15001 ccttcagggg ttggaaaagg aacggttaga agagagattt ttgagagttc tgaaaaccaa
    15061 tttcaatact ctgtatcgat gacgacacgc gcacaacgtc ctggagaagt ggacggtgtc
    15121 gactatttct tccgtactcg tgaagaattt gaagagctga ttcgtcaagg acagatgttg
    15181 gaatacgcag aatatgtcgg taactactat ggaactcctc tgacctatgt caatgaaacc
    15241 ttggacaagg gaatcgatgt tttccttgaa attgaagttc agggtgctct tcaggtcaag
    15301 aaaaaggttc cagatgctgt ctttatcttc ctgacaccac cagatttgga tgaattgcaa
    15361 gatcgcttgg taggtcgtgg aacagatagt gcagaagtga ttgcccaacg aatcgaaaag
    15421 gccaaggaag aaattgccct catgcgtgag tatgattatg ccattgtcaa cgatcaggtg
    15481 cccctagctg ctgaacgtgt caaatgtgta attgaagcag aacacttctg tgtggatcgt
    15541 gtcattggtc actatcagga gatgttacca aaatctccaa ctacccgata aaatttagaa
    15601 aataggtaca agcaaatgat gttaaaaccc tctattgata ccttgctcga caaggttcct
    15661 tcaaaatatt cactcgtaat cttggaagca aaacgtgccc acgaattgga agcaggtgcc
    15721 ccagcaactc aaggtttcaa gtctgaaaaa tcaactcttc gcgctttaga agaaatcgaa
    15781 tcaggaaacg ttacaattca cccagatcca gaaggaaaac gcgaagcagt gcgtcgccgt
    15841 atcgaagaag aaaaacgccg caaagaagaa gaagaaaaga aaatcaaaga gcaaattgct
    15901 aaagaaaaag aagatggtga aaaaatttaa ggttgggggg actcaatctt attttttcta
    15961 ttgcaagaat gtactgacaa ggaggaggtg agaagatggc cctagctaag attatcgtag
    16021 atgtgccctt gatgcagacg gaccagccct atagttacag gattccggag gaatttgagg
    16081 gaatgctgga agttgggatg cgggttcatg tgccttttgg taagggtaat cgcctgattc
    16141 aagggattgt tcttggtttg gagtcccaat cagatggaga agagatggag caagatttaa
    16201 aagatattgc agaggtgctg gatttttctc ctgttctcac gccagaacaa ctctggctgg
    16261 ctgaggagtt acgcaaatct gtcttctcct acaaaatttc tatcctcaag gccatgcttc
    16321 caggatttct gaattctagc tatgacaaga ttctctatcc tctggaaggc ttgagtcagg
    16381 aagaacgagt gcgcctgttt ggttcagaag attcgctagc cttttcttca ctagaccttg
    16441 ccaagcaagc tgaaatgatg cgtttgacta gaaaaggcct gcttggtctg gaatatcagg
    16501 cagtcgatca aaagaaggtc aagacccagt cttggtatga ggttgaccat gctcaattag
    16561 aaggtgttga gatttctaca cgtgccaaga aaaagttgga actgagagat tatctgctgt
    16621 ctcatccaga gagcgcttcc ttggctagct tgttagagtc ctactcgcga gagcaagtca
    16681 acttctttgt ggatcaaggt gctgttacca tagtccaaaa ggaagttcaa cgctcggctg
    16741 cttattttga aggaattgaa gcaagtagac ctttggagtt aaatccagaa caaagacagg
    16801 cgcgtgatgc ggttgtgagt tctattggca gttctcaacc tccctttcta cttcaaggga
    16861 ttacaggaag tgggaagacc gaggtttact tgcagattat ccaaggtgcc ctggataagg
    16921 gcaagacagc tattttgctg gtacctgaga tttccttgac tcctcagatg accgagcgtt
    16981 ttatagcacg ttttggggac aaggtagcca ttcttcactc aggattatcc aatggtgaaa
    17041 agtatgatga atggcgcaag gtggagcgtg gcgatgccca agttgttgtt ggtgccagat
    17101 ctgctatctt tgctcctctg aaaaatctgg gtgtcatgat tattgatgaa gagcatgaag
    17161 cggcttataa gcaggacagc aatccccgtt accatgccag agaggtagct attttacggg
    17221 ctcagtataa tcaagcaact ctggtacttg gatctgcaac gccgagctta gagagccggg
    17281 cacgtgctgg caaaggcgtc tatcaacact tacgtctaac ccaacgtgcc aatcctttgg
    17341 ctacaatccc tgaggttcaa gtgattgact ttcgagacta tatcggacaa aatgagacgt
    17401 caaactttac gcctcctttg ctagaggcta tccaagaccg tctggttaaa aaagagcagg
    17461 tggttctcat gctcaatcgc cgtggttatt ctagctttgt catgtgtcgg gagtgtggga
    17521 cggtggatac ttgtcccaac tgcgatattt ctctgacctt gcatatggat accaagacta
    17581 tgaactgcca ttattgtggt ttctcgaagg atattcctca ggtctgtcct aactgtaaga
    17641 gccacagtat tcgttactat gggacgggaa ctcagaaggc ttatgatgag ctagcagaac
    17701 tctttcccca agttcgcatt ttgaggatgg atgtggatac gactcgaaag aaaggcagtc
    17761 accaagcctt gcttgaccag tttgggcgag gggaagcgga tattttactt ggtactcaga
    17821 tgattgcaaa gggtttggat tttcccaatg ttaccctagt aggagttcta aatgcggata
    17881 cggccttgaa tctgcctgat ttccgttctt ctgagagaac cttccagctc ttgactcagg
    17941 tggcaggtcg agcaggtcgg gctgaaaaag caggtcaagt cttgatacag tcctacaatt
    18001 cgcagcacta tgctattcga tttgccaagg atcaagatta cgaaggcttt tatgcctatg
    18061 aaatgggaat cagacgacaa ctcggctatc caccttacta tttcacgatt ggtattaccc
    18121 tttctcacaa gaaagaagaa gaggttttca aacgtgccta tgaagtcatg aacattttgc
    18181 ggtcaggctt gtcagagacc agtcccattc tggggccaac gccaaaaccg attgcccgta
    18241 cccgcaacct ctatcattac cagattttaa ttaaataccg tttagaagat gagctggggc
    18301 cgaccctcaa ccaggtcttg gccttgactc aagaacggga aaatagtgag ctccgtctca
    18361 gcattgacca tgagccgcag caatttttat aagaaggaga agatatgaca aaactaatct
    18421 ttatggggac ccccgacttt tcagcaacag tcttaaaagg acttttgaca gatgaccgtt
    18481 acgaaattct agccgttgtg acccagccaa accgtgctgt tggtcgtaaa aaagttatcc
    18541 aagaaacccc agtcaagcag gctgccaagg aagcaggact atctatctac caacctgaaa
    18601 aattatctgg aagtccagag atggaagatc ttatgaagct aggagcagat ggaattgtga
    18661 ctgctgcttt tgggcagttt ctcccaagca aactccttga tagcatggac tttgctgtca
    18721 acgttcatgc ctccctcctt cctagacacc gtggtggtgc gcctatccat tatgccttga
    18781 ttcaagggga tgaggaagct ggtgtgacca tcatggaaat ggttaaggaa atggatgcag
    18841 gagatatgat ttctcgtcgc agcattccga tcacagatga ggacaatgtt ggcaccttgt
    18901 ttgaaaaatt ggcgctagtt ggtcgtgatt tgcttttgga cactctgcct gcctatattg
    18961 ctggtgatat caaacctgaa ccgcaggata cgagtcaggt taccttctct ccaaatataa
    19021 agccagagga agaaaaactg gactggaaca aaaccaatcg tcaactcttt aaccaaattc
    19081 gtggaatgaa cccctggcct gttgcccata ctttccttaa gggcgaccgc tttaagattt
    19141 atgaagccct accagtagaa ggtcagggaa atccaggtga gattctctct atctgcaaga
    19201 aagaattgat tgtcgcaacg gctgaagggg ctctatccct caaacaagtg cagccagctg
    19261 gtaagcctaa gatggacatt gcttccttcc tcaacggagt tggacgtaca ttgactgtag
    19321 gagaacgatt tggtgactaa agtagaaacg gctagaagtt tagctctagc agtgctagag
    19381 gatgtttttg tgaatcaagc atattcaaat attgccttaa ataaacacct caaggggagt
    19441 cagctctctg cagcagacaa gggcttagtg accgagctag tctatggaac ggtagccctt
    19501 aaactgactc tggaatggta cctatcccac tttatcgaag acagagacca gttagacagc
    19561 tggctctatg tccttcttct catgagtgcc taccaactcc gctatttgga caagattcca
    19621 gatcatgctg tggtcaatga agcagtggaa ttggccaaac tccgtaaaaa aggcagtgaa
    19681 aaattggtca acgctgtcct tcgccgtatc ttgcgtgaag gctggccaga tattgctagc
    19741 atcaagcgaa aaaacaagcg tgactccatt gcctattctc tcccagtttg gctagttgcc
    19801 aaactcaagg aagaatacgg agaggagaga gcaaaagcca tctttgaaag cctgttagtg
    19861 cgaaacaaag ccagtattcg tgtaacagac ctaagtcgaa aagaggaaat ccaagccttg
    19921 ttggaggcga ataattccct tttagcaacc actggtctgg ttaaggagca ggggcatttt
    19981 gcagggcata atttgtttgc gaatggagcc attaccatcc aagatgagtc cagtcagctg
    20041 gttgctccga cgcttgattt acaaggtgat gagcgagtgc ttgatgcctg tgcggctcca
    20101 ggtgggaaaa cagcccatat agcctcttat cttacgacag gtcaggttac tgctctggac
    20161 ttgtacgacc acaagttgga tttaattcaa gaaaatgccc aacgtctggg agttgcagat
    20221 cgggttcaaa ctcaaaaatt ggatgccaga aaggtgcatg agttttttga ccaggattcc
    20281 tttgataaga ttttggtgga tgctccttgt tcaggaatcg gtcttttgcg ccgaaaacca
    20341 gatatcaaat acaataaaga aacggcagat ttcgcgtcct tgcaggaaat tcagttagaa
    20401 atattaggta gtgtttgtca aatactaggc aaaggtggta taataactta tagcacctgc
    20461 actatcgtct cagaggagaa ttttcaagtc gttaaggcct ttttagagag tcatcccgag
    20521 ttcgagcagg taaaactaga acatgaatgt aaggatatca tgaaagacgg ctgtatcctc
    20581 attacacctg aattgtatgg aagtgatgga ttcttcatca gtcaatttcg caagatatcg
    20641 gattaggaag gaactgacac atggaaattt cattattaac agatgttggt cagaaacgaa
    20701 caaataacca agactatgtc aaccactatg tcaatagagc tggacgtacc atgattattt
    20761 tagctgatgg gatgggaggt catcgcgcag ggaatatcgc tagtgaaatg gcggtcacag
    20821 acctgggtgt agcttgggtt gatacccaga tcgatacagt caatgaagtg cgtgaatggt
    20881 tcgcccatta cctagaaatt gaaaatcaaa agattcacca gcttggtcag gatgaagctt
    20941 acagaggcat gggaactact ttggaagtcc ttgctattat tgataatcag gctatctatg
    21001 ctcatattgg tgattcgcgt atcggcttga ttcgtggaga agaataccat cagttgacga
    21061 gtgatcattc tttggttaat gaattgctca aggctggtca attgacacca gaagaggcag
    21121 aagctcatcc gcaaaaaaat attatcaccc agtctattgg gcaaaaagat gaaattcagc
    21181 ctgattttgg gacagttatc cttgagtcag gtgactatct cttgctcaat agtgacggct
    21241 tgaccaacat gatttcaggc agtgagattc gtgatattgt aaccagtgat attcctttag
    21301 cagataaaac ggagacactt gttcgttttg ctaacaatgc aggaggttta gacaacatta
    21361 cggttgccct tgtttctatg aacgaggagg atgaagaatg atccaaatcg gcaagatttt
    21421 tgccggacgc tatcggattg tcaaacagat tggtcgagga ggcatggcgg atgtctacct
    21481 agccaaagac ttaatcttag atggggaaga agtggcagtg aaggttctga ggaccaacta
    21541 ccagacggac ccgatagctg tagctcgttt tcagcgtgaa gcgagagcta tggcagatct
    21601 agaccatcct catatcgttc ggataacaga tattggtgag gaagacggtc aacagtatct
    21661 tgcaatggag tatgttgctg gactagacct caaacgctat atcaaggaac attatcctct
    21721 ttctaatgaa gaagcagtcc gtatcatggg acaaattctc ttggctatgc gcttggccca
    21781 tactcgagga attgttcaca gggacttgaa acctcaaaat atccttttga caccagatgg
    21841 gacggccaag gtcacagact ttgggattgc tgtagccttt gcagagacaa gtctgaccca
    21901 gactaactcg atgttgggct cagttcatta cttgtcacca gagcaggcgc gtggttcgaa
    21961 ggcgactgtg cagagtgata tctatgccat ggggattatt ttctatgaga tgttgacagg
    22021 ccatatccct tatgacgggg atagcgcggt gaccattgcc ctccagcatt tccagaaacc
    22081 cctgccgtcc gttattgcag aaaatccatc tgtacctcag gctttagaaa atgttattat
    22141 caaggcaact gctaaaaagt tgaccaatcg ctaccgctcg gtttcagaga tgtatgtgga
    22201 cttgtctagt agcttgtcct acaatcgtag aaatgaaagt aagttaatct ttgatgaaac
    22261 gagcaaggca gataccaaga ccttgccgaa ggtttctcag agtaccttga catctattcc
    22321 taaggttcaa gcgcagacag aacacaaatc aatcaaaaac ccaagccagg ctgtgacaga
    22381 ggaaacttac caaccacaag caccgaaaaa acatagattt aagatgcgtt acctgatttt
    22441 gttggccagc cttgtattgg tggcagcttc tcttatttgg atactatcca gaactcctgc
    22501 aaccattgcc attccagatg tggcaggtca gacagttgca gaggccaagg caacgctcaa
    22561 aaaagccaat tttgagattg gtgaggagaa gacagaggct agtgaaaagg tggaagaagg
    22621 gcggattatc cgtacagatc ctggcgctgg aactggtcga aaagaaggaa cgaaaatcaa
    22681 tttggttgtc tcatcaggca aacaatcctt ccaaattagt aattatgtcg gccggaaatc
    22741 ttctgatgtt atcgcggaat taaaagagaa aaaagttcca gataatttga ttaaaattga
    22801 ggaagaagag tcgaatgaga gtgaggctgg aacggtcctg aagcaaagtc taccagaagg
    22861 tacgacctat gacttgagca aggcaactca aattgttttg acagtagcta aaaaagctac
    22921 gacgattcaa ttagggaact atattggacg gaactctaca gaagtaatct cagaactcaa
    22981 gcagaagaag gttcctgaga atttgattaa gatagaggaa gaagagtcca gcgaaagcga
    23041 accaggaacg attatgaaac aaagtccagg tgccggaacg acttatgatg tgagtaaacc
    23101 tactcaaatt gtcttgacag tagctaaaaa agttacaagt gttgccatgc cgagttacat
    23161 tggttccagc ttggagttta ctaagaacaa tttgattcaa attgttggga ttaaggaagc
    23221 taatatagaa gttgtagaag tgacgacagc gcctgcaggt agtgtagaag gcatggttgt
    23281 tgaacaaagt cctagagcag gtgaaaaggt agacctaaat aagactagag tcaagatttc
    23341 aatctacaaa cctaaaacaa cttcagctac tccttaaaag cagatggata atcaaaatga
    23401 gtaatcaatc tttgtcatga tttcatggca aagatttttt ttgagtccag atttgtgata
    23461 gaatagaggg agttgataaa aggaggaaag catggaagaa tcaaaagaat taaatgccgt
    23521 cattgatgtg attatgctag cggggactat tctccttaaa agtggctcag aaatccatcg
    23581 tgtagaagat accatgattc gaatcgcgca ttcgcagggg attgtggatt gcaatgtcct
    23641 tgccatgcct gccgctatct ttttctctat tgaaaatacc aatatttcgc gcatgaagcg
    23701 cgtgacctcc tcttcttata acatcgaaaa agtctgcgat gtgaaccaga tttctcgtca
    23761 gctagttggg gggcagattg acttagaaac agcctttaag caattgacgg ccttgcaagc
    23821 tcaacccctt ccctatacta agttgcaggt aactctggct gcgaccttta gtgctccttt
    23881 cttttcagtt atgtttagcg gaaatatcta cgacgcactt ggtgcaggag tggcgacctt
    23941 atttggtttt gccttttccc tctatgtgga aaaatttatc cgaattccct ttgtgacagc
    24001 ctttgctgga gcctttgtct ttgggataat tgcccagttt tgggctcgct acacaggttt
    24061 tccttcaacg gcagatttga ttatagctgg tgcggtcatg ccgtttgtac caggtattgc
    24121 cttgaccaat gcggtccgtg atattatgac caaccacata aactctggta tgagtaagat
    24181 gtttgaatcc ctgctcatta ccctcgcttt aggggcagga acttctgtcg ccttggtatt
    24241 gatgaactaa tatgacacta acaacctttt tattacaagc agtagcaagt tttcttgcca
    24301 ttatcacttt tttaatcgta ctcaatgtgc aacggtctat gctcttacct ggagggattt
    24361 tgggcatgac tgtctggcta atctatctct tgctcaagga accgaccaat gtcattgtag
    24421 ctaccttcat tgcagccatt attggttctt gtgtcagcca gattttaagt attctttata
    24481 agacacctgc tgtggtcttt atcttggcca ttttggcacc gctggttcca ggttatctct
    24541 cctaccgaac aactgccttt tttgtgacag gggactataa taaagcactg gcaagtgcga
    24601 ccttggttgt catgttggct ttggtaatct ctattggaat ggctagcgga acagtgattc
    24661 tcagactgta tcattatata aaaacacatc gagtatcgta gactttacag aaataaaaga
    24721 attttctgaa aaatgagata aataaattaa caacgctttc tatatgtgcg agaataccgc
    24781 acttatgaag aaattgcgac tgattttggt atccacgaaa gcaacttact ccgtcggagc
    24841 caatgggttg aagtaactct tgttcaaagg tggtgttacg atttcaagaa ctcctctcag
    24901 ttctgaagac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc ctaaaaaaaa
    24961 caattagcga atgattctgg taaaaagaaa tttcacgcta tgaaggctca agcgattgtc
    25021 acaagtcaag ggagaattgt ttctttggat atcactgtga actattgtca tgatatgaag
    25081 ttgttcaaaa tgagtcgcag aaatatcgga caagctggta aaatcttggc tgacagtggt
    25141 tatcaagggc tcatgaagat atatcctcaa gcacaaactc cacgtaaatc cagcaaactc
    25201 aagctgctaa cagttgaaga taaagcctat aaccatgcgc tatctaagga gagaagcaag
    25261 gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat
    25321 catcgtaaac gcttcggatt acgaatgaat ttgattgctg gcattatcaa tcatgaacta
    25381 ggattctagt tttgcaggaa gtctactact ttccttattg tctgtaaatc tactgacctt
    25441 gttgtttatc ccagtcatgg tttctagttc gggctcagag tttcaaagtg gatggcaaga
    25501 gcatcaattg attgctgaga aggttagtaa aacacttgat aagacatttg ataaggatgt
    25561 cagagaaatt ccgaccagtc agttttatca aaaatttgta gatgagatgg gaaggactta
    25621 ctcaggaaat ttgatcctcc aggagctgat aactgtgaat ggagcttata aagctactta
    25681 tatcggtgaa ctctctagca actaatcatt ctttaaaatg atgaccaata agaggcttga
    25741 tttggtgaat caagtctttt ttctctcttt tactgccatt tgtgataaaa tagtatagaa
    25801 cgatttttta catgaatgat aaaacagagg taaatatgac aatcggtatt gataagattg
    25861 gttttgcgac cagtcaatat gtcttgaaat tacaagactt agcagaagcg aggggaattg
    25921 accctgaaaa attaagtaaa ggactcttac tcaaggaatt gagtattgcg cccctaactg
    25981 aggacatcgt gaccttggcg gccagtgcta gtgactctat tttaactgag caagaaagac
    26041 aagaagttga catggtcatt gtggcgaccg agtcaggaat tgaccagagt aaggctgcgg
    26101 ccgtctttgt gcatggcttg ctgggcatcc agccctttgc tcgtagtttc gagattaaag
    26161 aagcctgcta tggggcgact gctgccctcc attatgccaa attgcatgtg gaaaattctc
    26221 cggagtccaa ggtcttggtc attgcaagtg atattgccaa atacggtatt gaaactccgg
    26281 gagaacccac tcagggtgcc ggaagtgtgg ctatgttgat tacacaaaat ccacgcatga
    26341 tggcctttaa taatgacaat gtagctcaga cccgtgacat catggatttc tggcgaccaa
    26401 attactcgac aactccttat gtaaatggtg tctattctac ccaacaatac ttggatagtt
    26461 tgaaaacgac ttggcttgaa tatcaaaaac gctaccagct tactttggat gattttgcgg
    26521 ctgtttgttt ccacttgcct tatcctaaat tagcgctaaa aggcttgaaa aaaatcatgg
    26581 ataagagcct gcctcaagag aaaaaagacc tcttacaaaa gcattttgac cagtctattc
    26641 tctacagtca aaaggtgggg aatatctaca caggttcact tttccttgga cttttgtctc
    26701 tcttggaaaa tacagatagc ttgaaagctg gggataaaat cgccctttat agttacggaa
    26761 gtggagctgt ggctgagttc ttcagtggtg aattggttga aggatatgaa gcttatttgg
    26821 ataaagaccg cttgaacaag ctcaaccaac gaactgtctt atccgttgca gactatgaaa
    26881 aggtcttttt tgaggaagta aacttggatg aaacaaactc tgcccagttt gctggctatg
    26941 aaaatcaaga ttttgccttg gttgaaattc tcgaccacca acgccgttat agcaaggttg
    27001 aaaaataatg aagataagtt ggaatgggtt ttctaaaaaa tcataccaag agcgcctcga
    27061 gctgttaaaa gctcaggcgc tccttagtcc tgagagacaa gctagtctgg agaaggatga
    27121 acagatgagt gtgactgtgg cagaccagct gagtgagaat gtagtgggaa ctttttctct
    27181 gccttattcg ctggttccgg aggtacttgt caacggtcag gaatacaccg ttccctatgt
    27241 gacagaagaa ccctctgtgg ttgcggcggc cagctatgcc agcaaaatca tcaagcgtgc
    27301 aggtggtttt actgcacaag tccatcagcg ccagatgatt gggcaggtag ccctttatca
    27361 agttgctaat cctaaactag cgcaagagaa gattgccagc aagaaagcgg agctcttgga
    27421 gcttgccaat caagcctatc cttctatcgt taaacgtggg ggtggggcgc gtgacctgca
    27481 tgtcgagcag ataaaaggcg aaccagactt tctcgttgtt tatattcatg tcgataccca
    27541 ggaagccatg ggtgccaata tgctcaacac catgctggaa gccttgaaac cagtcttaga
    27601 agaactcagt cagggacaga gtctcatggg aatcctgtcc aactacgcga ccgattctct
    27661 ggtgactgca agctgtcgca tggcctttcg ctacttgagc cgccaaaagg atcaaggacg
    27721 agagattgcg gagaaaattg cgttggctag tcagtttgcg caggatgatc cttaccgagc
    27781 tgctactcat aataaaggaa tttttaatgg tattgatgcg attttgattg ccactggtaa
    27841 tgactggcgt gccatcgaag ctggggccca tgcctttgcc agtcgagatg gacgctatca
    27901 aggtcttagt tgctggacgc tggaccttga aagagaagaa ttggtcggtg agatgaccct
    27961 gcccatgcct gtagcgacta agggtggctc tatcggcctc aacccacgtg tagctctcag
    28021 tcatgatcta ctaggaaatc cttctgccag agaattagcc cagattatcg tgtctatcgg
    28081 tcttgcccaa aattttgcag ccctcaaagc cttggtaagt acgggcatcc agcaaggcca
    28141 catgaaacta caggccaaat ccctagctct cctagctggg gctagtgaat ctgaagttgc
    28201 tcccctagtc gagcagctta tcgcagataa aacctttaac ctagagacag cccagcgcta
    28261 tctcgaaaat ttaagatcat aaaaaactca gacgaatcgg tctgagtttt cttgtgttta
    28321 aatacttttt cctggtaata gtgtaactgg taagaagtaa ccagttgtgg tgacttcctt
    28381 gccttcaatc ttttgcaaga gaagatcaat agtgagacaa gcaatctctt ccaaaggttg
    28441 cttgatagta gccaattgag ggtagtaatt ttcgataaag taggtcccat catagccgat
    28501 gaccttgagc tcttttggga cagaaatgcc caattcttga gcgattttaa tgaccagaat
    28561 agctgtcaaa tcatccgaag caaaaatggc atctggtttt tcccgggtca agatattctt
    28621 gatttccatt tcttttctga cgggagaaaa gtcactggaa acattgataa taggagcttt
    28681 tgggagtacg gatgcaaaac cagcgtggcg cagtccggtt ggcgaattgg aattgtcatt
    28741 ccctgtaatc atgatgatag actgggcacc tgtcttgacc aaggtttggg cagcaagaac
    28801 cccaccagca tagttgtcag aggagacgac agggatgtct ggcgataggt ttcggtcaaa
    28861 ggaaataatc ggcgctgtca cacgattgta gtcttcgatt cctaggttgt gactaccaga
    28921 aatgatgccg tccacctgat tggcttccaa catttcgatg tattcacgtt ccttctcaga
    28981 atcatgttca ctgttgcaga tgatggtctt gtaaccattt ttgaagagtt ggtgttccaa
    29041 tttatcaatc aattctgcat agaaaacatt ggaaatattg gggaaaatca agccgattaa
    29101 cttagctgat tttccttgca gactacgagc caggttgttg ggtttatagc ccaattctcg
    29161 catggcttca ttgacttttt ggatggtttt ctcagataga tacccttttt tattgataac
    29221 ccgagaaacg gtagtaggac tgacgcctgc aagtttggcg acatcagtta gttttgcgac
    29281 cataatctaa ttcatagtaa gttccagttg ggtttccaga tttgatcagg ataccatttt
    29341 ggtccgcatg tgggaagaca cgaccagaaa atactttttc tcctttattg atgaaaattt
    29401 caaagacaga gttatcgatg aagattgtag cagtagtagc ctgattctcg atagggcaag
    29461 aacgagttgt cccaaattct tgggcatact gttctccagc ctggctacga tccactgtca
    29521 cttgaccgtt tacaaggtca aagttgattg aaagtccctt accttcttta tcagcaagta
    29581 agacaatctc gctctggcta ttagcttcca agttgagttc aagttcgtaa gtgttcttgg
    29641 tttgggaacg gtttgagaag gcttcttcag aagcacgaag gtccttaata gcagcgactg
    29701 gatactggta gagcttgtcg tctttgatag tgagttcctt gaccaaagag aaggttcctt
    29761 ggtggtcaaa acggtcagat gggtaagaaa catctggcaa accaagccag ctaactgcta
    29821 gagcacgccc atcaggagcg ttgaaggctt gagttgcata ggcttcgaaa ccgtaatcca
    29881 tgttttgaag ttgagacaca tctaccattt tggcattttt agggtcaaag gaagccccaa
    29941 tcttatacat atttggaaag atattatcgt agtctagaac tttcttatcc aatccttgtg
    30001 gacagtagag aaggacaggt tgttcctcta caaagaccag attaggacat tccatcatgt
    30061 aggcagtacg gtcgttagca aagtcaaggt cgccaactgc ttgccagttt gtgtagtcgt
    30121 tattgacagc cttgtagaga cgaacgaaac cttttttctc caagtcctgt ccaccgacga
    30181 cagcatagta ttgaccttta aagttaaaaa tttgtggatc gcggaagtgg tcagtagagt
    30241 ctgctggctg gtcaatcaag atcttgtcaa tctttgtaat cttaccctcc ttgtccatca
    30301 aagcaccgat ctggtatggg tgacggatcc agttttcatc gcgaacattt cctgtataaa
    30361 ataggaataa gttatcgcca aattgcatgg cagaaccaga gtaggcaccg tggctatcta
    30421 atggagtatc tggtaaaact ttgattccag tttctttaaa gtgaaccaaa tcatcacttt
    30481 ctagctgtgc ccaagatttt aaaccgtggg ctgcaccaaa aggaaaattc tggtaaaaga
    30541 ggatccactt gccatcaaag taagaaaagc catttgggtc gttgagaagt cctgtttttg
    30601 gctcaacatg gtaatgagta tgccatggag attgtgccat attttccttt atatgttgaa
    30661 tttcttcttg cgtccaatct tgataaagtc tgtaacgacg ctcagttgtc cattccattc
    30721 attcctccaa aaatcttatc acttttaata gtaactgttt tcgataaaaa atgcaagcct
    30781 tttatgttaa aaaagaggaa aaactgtcaa aggattatca taaaataaat acaggaaatc
    30841 aatcaaattt ttacgaaaat gtgaaagata atgaaataaa agacttttta accaaggttt
    30901 caaacttatt tttcaaggaa ttcctgatga aacagtcaaa aacaatcaat ctaaagcttg
    30961 cctactaagt ggatagaata tttttctgca aaacgcttga catattttta aaacatgata
    31021 aaataatagt cgtaagggcg aataaaatcg ctttcaaaca agaacaaaat gttatataag
    31081 gagatttttg caaatgaaca atcaggaaat tgcaaaaaaa gtcatcgatg ccttgggcgg
    31141 acgtgaaaat gtcaatagtg ttgcccactg tgcgactcgt ctacgtgtca tggtcaaaga
    31201 tgaagagaaa atcaataaag aagtgattga gaacttggaa aaagttcaag gtgctttctt
    31261 taactcaggg caataccaaa ttatctttgg tacaggtaca gttaacaaaa tgtacgatga
    31321 agttgttgta cttggcttac caacatcatc taaggatgac atgaaagcag aagttgctaa
    31381 acaagggaac tggttccaac gtgctatccg tacttttggt gatgttttcg ttccaatcat
    31441 cccagttatc gtagcgacag gtctcttcat gggtgtgcgt ggtcttttca acgctcttga
    31501 aatgccactt ccaggtgact ttgcaactta cacacaaatc ttgacagata cagccttcat
    31561 catcttgcca ggtttggttg tgtggtcaac cttccgtgta tttggtggaa atcctgccgt
    31621 tggtatcgtt cttggtatga tgcttgtctc tggctcactt ccaaacgctt gggcagttgc
    31681 tcaaggtggt gaagtaacag cgatgaactt ctttggtttc atccctgttg ttggtttgca
    31741 aggttccgtt cttccagcct tcatcatcgg ggttgtcgga gctaaatttg aaaaagctgt
    31801 ccgcaaggtt gttccagatg tcattgacct cttggtaaca ccattcgtga cacttttggt
    31861 catgtctatc cttggactct ttatcattgg accagttttc cacgttgttg aaaactacat
    31921 ccttattgct acaaaagcga ttcttagcat gccatttggc cttggtggtt tcttgattgg
    31981 tggggttcac caattgatcg tcgtgtcagg tgtgcaccac atcttcaact tgcttgaagt
    32041 gcaattactt gctgctgacc atgctaaccc attcaacgct atcatcacag ctgctatgac
    32101 agctcaaggt gctgctactg ttgcggttgg tgttaaaaca aaaaatccaa aactgaaaac
    32161 acttgctttc ccggctgctc tttctgcctt cctaggtatt acagagcctg ctatcttcgg
    32221 ggtgaacttg cgcttccgta aaccattctt cctttcattg attgctggtg caatcggtgg
    32281 tggattggct tctatccttg gacttgctgg tactggtaat ggtatcacca tcatccctgg
    32341 tacaatgctt tatgttggta acggacaact tccacaatac cttcttatgg tagctgtatc
    32401 atttgccctt ggttttgctc ttacttacat gtttggttac gaagatgaag tagacgcaac
    32461 tgcagctgca aaacaagctg aagtggctga agaaaaagaa gaagttgcgc cagcagctct
    32521 tcaaaatgaa acacttgtaa ctcctatcgt cggtgatgtt gtcgctcttg ctgatgtcaa
    32581 tgacccagtc ttctcaagtg gagctatggg acaaggtatc gctgtgaaac caagccaagg
    32641 cgtggtctat gcaccagctg atgctgaagt ttcaattgcc tttccaacag ggcacgcttt
    32701 tggtttgaaa acaagaaatg gtgctgaagt tttgattcat gttggtattg atactgtatc
    32761 tatgaacggt gacggttttg aaacaaaagt tgctcaaggt gataaggtga aagttggcga
    32821 tgttcttgga acatttgact caaacaaaat cgctgcagct ggacttgatg atacaacaat
    32881 ggttatcgtt acaaatacag gtgactacgc ttcagtagct ccagtcgcaa caggttcagt
    32941 tgctaagggg gatgctgtga tcgaagtgaa aatctaatca atcctctcta atgtgaaaac
    33001 gaacaaatga catatttgtt cgtttttatt agatagtaag atttacagag gaaaatctaa
    33061 aaaatagaga catttagact ttcgaagtat gctataataa agaaaataaa aacaagaggt
    33121 ttatcatgac aaaattatat ggaagcttgg aagcgggcgg tacaaagttt gtctgtgctg
    33181 tcggtgatga aaactttaac gttgtagaaa aaacacaatt tccaacaaca actccaatcg
    33241 aaacaatcga taaaaccatt gagttcttct caaaattcga taaccttgct ggtcttgcag
    33301 ttggttcatt tgggccgatt gatattgaca aaaactcaaa aacttatggc tttatcacga
    33361 cgactccaaa accaaactgg gcaaatgtgg acttgcttgg tgcccttcgt cgcgccctaa
    33421 atgtgccaat gtacttcaca acagacgtaa acagctctgc ttacggtgaa gtggttgccc
    33481 gtaacaatgc tggtggtcgt atcgaaaact tggtttacta cacaatcggt acaggtatcg
    33541 gtgcaggtgt catccaacgt ggtgagttta tcggtggtgt gggtcaccct gaaatgggtc
    33601 attattatgt tgctagacac ccaatggata ttgaaaaaga gtttaagggt gtttgtcctt
    33661 tccataaggg atgtctagaa ggttatgcag ctggtccaag tttggaagct cgtacaggtg
    33721 tacgtgggga aactattgaa ctcaacaacc ctgtttggga tgttcaagcc tactatatcg
    33781 ctcaagctgc ggttaatgcg acagtgactt tccgcccaga cgtgattgtc tttggtggag
    33841 gggtcatggc tcaacaacat atgctggacc gtgtccgtga gaaatttaca tctcttctta
    33901 atggttacct accagtacca gatgtgcgtg actatatcgt gacgccagca gtcgcaggaa
    33961 atggttctgc cacacttggg aactttgttc ttgcaaaaga agtttcaaaa taaagcacaa
    34021 aatccgcttg ggaaaccaaa cggatttttt acgtgtcaaa gcatttgcca gaaaaagtca
    34081 ataatatagg tcagaccgta ggtataaacc acgagggtaa agggtttggt cagaatgatg
    34141 atggtcatgt agcgcttgaa actcatcttg gtcagggcag ccagcataca gagaaagtca
    34201 gctgggctaa tgggccaaat catcataaaa ataaagaagc ggtcaaaacg attgccctta
    34261 tctagccagc cgatgtactt gtcgtaggtg cgcttgctga cgacagactg gacaaaggca
    34321 gctccgtata ggcgcactag ataaaagata atggcacagc caatcacgat gccgatatag
    34381 ttgtagatag tcccgatgat gtgcccgtag ataaagaccc cagccaccga ggtcaaggcc
    34441 cctggaatga tagggacgac agtctgtaaa atctgtaaaa agataaagag aggtggaccc
    34501 cagatgcctg cctgctggat aaaggcagag agggtttcct tggattgtaa aatcccagcc
    34561 tgataagccc aaatacagaa aatcaaactc ccaaccccac cgacaatcga tgagatattg
    34621 ataactcgct gcagaagggg agaaacagct ttcatttgtt tatcctgtga catgtaaact
    34681 cctcatagat agtatatttc tactatacca tattttggag gagaaagggg gaatgagtct
    34741 tttttgataa ggaatagaaa gtaaatagcc cttcgagact tagaataggt ctgtcaacta
    34801 aaggttatat gcattctagg tttcgaataa tcgcaattcc ataaatttaa aagtattcta
    34861 agtttcgaat agcttatctg ggataatttt gaggcattcg cgctttcgaa tagtcatttt
    34921 ccctaaagtt tatatgtatt ctgaagctag aaagactaaa atataaaagt agtaagagtc
    34981 tctctctgat ggacgtaatt tatggctagt atgaatcctt tgaattgtta gttaatttta
    35041 atattaatca gttctgaaat aattactaaa tatagttaga tggtgagtta attattgcta
    35101 aatttcaagt tcaagttagt catccagaag tcttctctgg gggacttgta gttcaagcat
    35161 tttttaggat agttgttaat ccacttttcg ataaatgcga cttcctttgt agttgttttc
    35221 ttagttccct gaggtaacca tctatgaatg agcctgttgt gattttcatt agatcctata
    35281 gtgatgtgca taatagatat agcagattga aactagaata gtatacatct acttctaaaa
    35341 cattgttaga aatcaatttg actgtcctga tcaatttgtc ctgtttttat ttcattttac
    35401 tatataatcc ttagaaaaca tatcggacaa gcggttgaat tccgttccgt ccattagtag
    35461 ataaaggatt actccttctt gaaatgacag atgaacttgt ttcttataaa aatgttgtct
    35521 gaaactatta gcctttttcc agaagtttta gaggaagaaa actttacgag gaaaaaggag
    35581 ttactcaaat aacaattcag gattaaaaat agacagttga ggagcggaag gtataaatta
    35641 agtttgctac tgtataatgg atttatcact aatactcttc gaaaatctct tcaaaccacg
    35701 tcagcttcgc cttgccgtag atatagttac tgacttcgtc agttctatcc acaacctcaa
    35761 aacagtgttt tgagctgact tcgtcagttt tatctgcaac ctcaaagctg tactttgagc
    35821 agcctacggc tagcttccta gtttgctctt tgcttttcat tgagtataaa agtgctccag
    35881 gccatatttt acacagcaat agtcttctaa aaatggacaa aaacattgta aaggctatca
    35941 aaaaggagta taatgagtgc aagaaatttc ggaggtgaaa gatgctagaa gtaagaagtc
    36001 tagagaaaag ttttggctcc aagcaagttt tgtttggtat tgactttcaa gcgcgaccag
    36061 gtcgtatttt gggactagtc ggaaaaaatg gtgctggaaa aacaacgatt ttccatagta
    36121 ttttgaaatt cctagaatat cagggagaaa tcggtctgga tggtcaggat attcgtcagg
    36181 agacctatgc tcggattggt tatctgcctg aagaacgcag tctcatgcct aaattgacag
    36241 ttcttgaaca agttcgctac ttggcgactc taaaaggcat ggatgccaaa gaagtcaaag
    36301 aaaaactccc tcaatggatg aagaggttgg aagtgaaagg aaagctgaca gataaaatca
    36361 agagtctgtc aaaaggaaat cagcagaaga ttcagctcat tattactctg attcatgaac
    36421 cagacctgat tatcttggat gagcctttta gtggattgga cccagttaat acagaattgc
    36481 tcaaacaagt catttttcag gaaaaagagc gcggagcaac cattatcttt tctgaccatg
    36541 tcatgaccaa tgttgaggaa ctttgtgacg atattctgat gatccgagat ggccgtgtgg
    36601 tcttgcacgg accagttcag gatgtccgca atcaatacgg gaaaacgcgt ctctttgttt
    36661 caagtgaacg aagcaaggaa gaattggaaa atcttcctca tgtcaaacag gtgagcttga
    36721 ccaaacaagg cagttggaaa ttgattttgg aggatgagag cgctggaagg gaactcttcc
    36781 caatcttgac tcaaggtcaa tatatcgcaa catttgacca acaagcgcca actatcgatg
    36841 aaatctttaa actagaatca ggagtggaag tatgagaaat atgtgggttg taatcaagga
    36901 aacctatctt cgacatgtcg agtcatggag tttcttcttt atggtgattt cgccgttcct
    36961 ctttttagga atctctgtag gaattgggca tctccaaggt tcttctatgg ctaaaaataa
    37021 taaagtggca gtagtgacaa cagtgccatc tgtagcagaa ggactgaaga atgtaaatgg
    37081 tgttaacttc gactataaag acgaagcaag tgccaaagaa gcaattaaag aagaaaaatt
    37141 aaaaggttat ttgaccattg atcaagaaga tagtgttcta aaggcagttt atcatggcga
    37201 aacatcgctt gaaaatggaa ttaaatttga ggttacaggt acactcaatg aactgcaaaa
    37261 tcagcttaat cgttcaactg cttccttgtc tcaagagcag gaaaaacgct tagagcagac
    37321 aattcaattc acagaaaaga ttgatgaagc caaggaaaat aaaaagttta ttcaaacaat
    37381 tgcagcaggt gccttaggat tctttcttta tatgattctg attacctatg cgggtgtaac
    37441 agctcaggaa gttgccagtg aaaaaggcac caaaattatg gaagtcgttt tttctagcat
    37501 aagggcaagt cactatttct atgcgcggat gatggctctg tttctagtga ttttaacgca
    37561 tattgggatc tatgttgtag gtggtctggc tgccgttttg ctctttaaag atttgccatt
    37621 cttggctcag tctggtattt tggatcactt gggagatgct atctcactga ataccttgct
    37681 ctttattttg atcagtcttt tcatgtacgt agtcttggca gcattcctag gatctatggt
    37741 ttctcgtcct gaggactcag ggaaagcctt gtcgcctttg atgattttga ttatgggtgg
    37801 tttttttgga gtgacagctc taggtgcagc tggtgacaat ctcctcttga agattggttc
    37861 ttatattccc tttatttcga ccttctttat gccgtttcga acgattaatg gctatgcggg
    37921 gggagcagaa gcatggattt cacttgctat tacagtgatt tttgcggtgg tagcaacagg
    37981 atttatcgga cgcatgtatg ctagtctcgt tcttcaaacg gatgatttag ggatttggaa
    38041 aacctttaaa cgtgccttat cttataaata gaagagcctt gcgaaagcga ggtttttaaa
    38101 gatgaaaagg atggaatcac gaacctaatt atgtacacat tatgtaaaca ggttgcttga
    38161 aaactcaatt gtatagtgaa ctgaatctgg aataggataa tattacttct aaaacattct
    38221 tataaattga tttgaattcc aaaatcccat cgttccgttc ttatttcatt ttgcgatata
    38281 gtgaaaggaa ataagtcagc tcacaaaaaa cgcataatat caaggatttt taacacttga
    38341 tactatgcgt tttctaattt taaagacttt ttgcctggtc tcttttgttc tatgagtatg
    38401 ttacatatct aggtaaaaat tatttaaagc aaattaacct tatttctcaa gatgtagtag
    38461 gttccgagat aaaagatagc tatgaatatg agttctttaa caatggctat acctgctccc
    38521 atatagaaat ggtcattgag tcctaccagt gaattgacat agaaattaga actaagattg
    38581 aagaaaagtt caataaatcc aatgacgatt tggataccaa tgtaggctgc aacagcgagt
    38641 gctgtacggt attcattgaa aagctgtcca atggaaatag ccaggtagat gcagaggatt
    38701 cctgaaatgg tatttagtag gaaggatata cctgtaagaa agatctgagg gagatgtgtt
    38761 tctacaaatg taatcacata agaaagagga atccattctg gagctgttaa agccagaata
    38821 ataacagcac ttagagccaa tacagcggtg ctaatcaatg accagataaa ggcaccgatt
    38881 agtttggctg tgatgatatg gtgttcagaa actggcaagg tcaaagtcag atagccttgt
    38941 cggtcgtaga cactaccttt gaagcgttta ataatcaaga aaatggttga aatcgcaagt
    39001 gtaagcatca agccaccaaa gactgtagct agaaaaagta gcatagtagc ctgactttct
    39061 tggtaaggta gatttttaaa gccttgtgtc tgtattccga tgagggcaga aaggactagc
    39121 acggctgcgt agagggctaa ataccacttg ttaacatttt taaattcgta gtgaactaaa
    39181 ttccaaaaca taaataatct cctttgctta ggccttaaat tcctgacgga agagttggtc
    39241 aatggattca cctgactcgt agcgaatatc atctacattt ccttgacgga cgacttttcc
    39301 gtcttttagg aagacaattt catccaagat tggctcgata tcagaaatca agtgggtaga
    39361 aatcaaaacg gtagaagttg gtgagtagtt gttgataatg gtattgagga tataagcacg
    39421 ggctgctgga tccaccccac caatgggttc atccaaaaca tagagacgag catcacggct
    39481 cataaccaaa atcagttgaa ccttttcttt gtttcctttt gatagtttct tgagacgact
    39541 attttcatca atgcccaggt ctgcaagtag atgatgagcg cgttcaagat tgaaatcttt
    39601 atagaaggtc ttgaagtagg ttagggcttc tttgaccttc atttgctcat tgagataggt
    39661 cgtatcaggc aaataagcta caacggcctt ggttgctggg cttgggtcca tgtcgttgat
    39721 gaggacacgt ccttgatctg gttgtaagag gccattaatt agtttaatca gggttgtttt
    39781 tcctgagccg tttggcccaa gaaggccgac aatttttcca gctggaatgt caagagaaac
    39841 attttcaagg gctggtgttg ctccatatga tttggataca ttttcaaata ctagtaatga
    39901 cataggctta aactccttta atataatcac tgactacgcc tggtagttct tctttttcat
    39961 agccaaaatg gatcatggag gaaacgaagt gttccaattc ttcttctgat aattgtttgc
    40021 gtgactgagc gattagctcc ttatccttag tcacaaatcg tccagttgtt cgcttgctgt
    40081 agacaaatcc ttctcgttca aggtctgata aggctctttg gatggtattg ggattgacac
    40141 cagcctcgct agctagctcc ctcacggttg gaagttgttg attgggttcc agtgtatggg
    40201 aaacaatctg aagcttgatt ttctccataa tctgtaaata gatgggtttt ttgttgtcaa
    40261 atgtccagga catcttagtc tcctttctct tatccttttg tcttaactaa ataatacaac
    40321 aaaacaaaaa acttgtcaag caaaaagaag aattgttttg ggaatcgctt aaaaaatggt
    40381 ataatgaaaa gaaaacgata aggagggaaa ggatgcgttg tccaaaatgt ggggctacca
    40441 agtcaagtgt tatcgatagt cgccaagcag aagaagggaa caccattcgt agaagacgtg
    40501 agtgcgacga atgccaacac cgttttacaa cctacgaacg agtagaagaa agaaccttag
    40561 tggttgttaa aaaagatggt acacgggaac aattctccag agataaaatc tttaatggga
    40621 ttatccgctc agcccagaaa cgtcctgtgt caagtgatga aatcaacatg gtagtcaatc
    40681 gtatcgaaca gaaactccgt ggtcgaaatg aaaatgaaat tcaaagtgag gacattggtt
    40741 cactcgtcat ggaggagttg gctgaattgg acgagattac ctatgtacgt tttgctagtg
    40801 tctatcgtag ttttaaggat gtcagtgagt tagagagctt gctccaacaa atcacccagt
    40861 cctctaaaaa gaaaaaggaa agataaatga agccaattga ccgtttttct tatctaaaga
    40921 ataatcgggt gtcgcaagat acctcatctc tggtacagtg ctacctcccg attatcggtc
    40981 aggaggcact gagcctttat ctttatacaa tcagtttttg ggataatggt agaaaggaat
    41041 atcttttttc aagtatcctc aatcatctca actttgggat ggatagactg ataaaatcat
    41101 tgaaaatctt atctgctttt aatctcttga ccctctatca aaagggggat gtttatcagc
    41161 tagccctcca tgctcctcta tctagtcaag acttcttgga gcatcctgtt tatcgcagac
    41221 tcttagagaa aaagattggc gactctgctg tggaggattt gaaagttgaa agtgctgatg
    41281 gtgaagaaat acctgtctca ctcaatcaag tctttccaga attggcagaa ctaggcagtc
    41341 aagaagacct tggtctcaag aagaaagtgg ccaatgattt tgacttggaa cattttcgcc
    41401 agctgatggc tcgagatgga cttcgttttg cggatgagca gtccgatgtc ttaaacctct
    41461 ttgctattgc cgaggagaag aaatggactt ggtttgaaac ctatcaattg gccaagtcaa
    41521 cagctgtttc tcaggttatt tcaaccaaac gcatgcgtga aaaaattgct caaaaaccag
    41581 tttcctctga ctttaatcct aaggaagcaa ccattatcaa ggaagctaaa agtaagactg
    41641 ccctgcagtt cttagcagaa atcaagcaaa cacgcaaggg gaccattacc caaacagaaa
    41701 gagaactttt gcaacagatg gctggcttgg gcttgctgga cgaagtcatc aatatcattc
    41761 tcttgttgac ctttaataag gtggattcgg caaacatcaa tgagaaatat gccatgaagg
    41821 tggctaatga ctatgcctat caaaagatac attcagcaga agaggccgtt ttacgaattc
    41881 gtgagcgagg gcagaaaaca aaaacacaaa aacagaatca gactgcccca gcaaaaacca
    41941 atattcctaa atggagtaac ccagattata agaatgaaac cagcgaggaa actcgtctgg
    42001 aactagaacg taagaaacaa gaactattag ctcgattaga aaaaggagga gattagatgg
    42061 aaagtgtcgg agacgtactc aaacgtcaac ccagccgttt ttattatcaa gatttggtcc
    42121 agaaaatcat gaaggaccct gatgttgcgg cttttatcca gcaagaatcc ttgactccag
    42181 aggaattaaa tcgcagtatc tccaagttta atcagtacat caccgagcgt gacaagtttc
    42241 tccgtgggga tacggattat attgccaaag gctacaagcc gattttggtt aagaatcatg
    42301 gctatgcaga tgtttcatat gaagaaactc ctgagctaat cgcggctgaa aaagaagcgg
    42361 ctattaagaa tcgtctcaag ctaatcaatc taccagccag tctcaaaaaa gctagtttgg
    42421 ctcaagttga tttggatgat ttgggacgct tgccagtttt tgaaaaacta ttagcctttg
    42481 tggagcaata tccagctatt cgaaaaggtc tttacttata tggagacttt ggtgtgggta
    42541 aaagtttcat ggtggcggcc ttagctcatg atttatcaga aaaacgtggt gtttcatcaa
    42601 ctctcctcca ctatcctagc tttgtcattg atgtcaaaaa tgctatcagt gatggcaatg
    42661 ttaagacctt ggtggatgag attaagctgt ctgaggtcct gattttagat gatattggcg
    42721 ccgagcaatc aaccgcttgg gtgcgtgacg aaatcctgca ggtcattctc caatatcgga
    42781 tgcaggaaaa tttaccgacc tttttcacat ccaacttcaa ctttgaagat ttggagaagc
    42841 attttgcgaa agtgaaacat ggaaatgacg aaacctggga agccagacgg gtcatggaac
    42901 gcatccgtta tttggctgag gagactcgtt tagaaggagt aaaccgtcga tgacagaaac
    42961 cattaaattg atgaaggctc atacttcagt gcgcaggttt aaagagcaag aaattcccca
    43021 agtagactta aatgagattt tgacagcagc caagatggca tcatcttgga agaatttcca
    43081 atcctactct gtgattgtgg tacgaagtca agagaagaaa gatgccttgt atgaattggt
    43141 acctcaagaa gccattcgcc agtctgctgt tttccttctc tttgtcggag atttgaaccg
    43201 agcagaaaag ggagcccgac ttcataccga caccttccaa ccccaaggtg tggaaggtct
    43261 cttgattagt tcggtcgatg cagctcttgc tggacaaaac gccttgttgg cagctgaaag
    43321 cttgggctat ggtggtgtga ttatcggttt ggttcgatac aagtctgaag aagtggcaga
    43381 gctctttaac ctacctgact acacctattc tgtctttggg atggcactgg gtgtgccaaa
    43441 tcaacatcat gatatgaaac cgagactgcc actagagaat gttgtctttg aggaagaata
    43501 ccaagaacag tcaactgagg caatccaagc ttatgaccgt gttcaggctg actatgctgg
    43561 ggcgcgtgcg accacaagct ggagtcagcg cctagcagaa cagtttggtc aagctgaacc
    43621 aagctcaact agaaaaaatc ttgaacagaa gaaattattg tagaaagtga gaaattatgg
    43681 ccctaccaac tattgccatt gtaggacgtc ccaatgttgg gaaatcaacc ctatttaatc
    43741 ggatcgctgg tgagcgaatc tccattgtag aagatgtcga aggagtgaca cgtgaccgta
    43801 tttatgcaac gggtgagtgg ctcaatcgtt cttttagcat gattgataca ggaggaattg
    43861 atgatgtcga tgctcctttc atggaacaaa tcaagcacca ggcagaaatt gccatggaag
    43921 aagcagatgt tatcgtcttt gtcgtgtccg gtaaggaagg aattaccgat gcagacgaat
    43981 acgtagcccg taagctttat aagacccaca aaccagttat cctcgcagtc aacaaggtgg
    44041 acaaccctga gatgagaaat gatatctatg atttctatgc tctcggtttg ggtgaaccat
    44101 tgcctatctc atctgtccat ggaatcggta caggggatgt gctagatgcg atcgtagaaa
    44161 atcttccaaa tgaatatgag gaagaaaatc cagatgtcat taagtttagc ttgattggtc
    44221 gtcctaacgt tggaaaatca agcttgatca atgctatctt gggagaagac cgtgttattg
    44281 ctagtcctgt tgctggaaca actcgtgatg ccattgatac ccactttaca gatacagatg
    44341 gtcaagagtt taccatgatt gatacggctg gtatgcgtaa gtctggtaag gtttatgaaa
    44401 atactgagaa atactctgtt atgcgtgcca tgcgtgctat tgaccgttca gatgtggtct
    44461 tgatggtcat caatgcggaa gaaggcattc gtgagtacga caagcgtatc gcaggatttg
    44521 cccatgaagc tggtaaaggg atgattatcg tggtcaacaa gtgggatacg cttgaaaaag
    44581 ataaccacac tatgaaaaac tgggaagaag atatccgtga gcagttccaa tacctgcctt
    44641 acgcaccgat tatctttgta tcagctttaa ccaagcaacg tctccacaaa cttcctgaga
    44701 tgattaagca aatcagcgaa agtcaaaata cacgtattcc atcagctgtc ttgaacgatg
    44761 tcatcatgga tgccattgcc atcaacccaa caccgacaga caaaggaaaa cgtctcaaga
    44821 ttttctatgc gacccaagtg gcaaccaaac caccaacctt tgtcatcttt gtcaatgaag
    44881 aagaactcat gcacttttct tacctgcgtt tcttggaaaa tcaaatccgc aaggcctttg
    44941 tttttgaggg aacaccgatt catctcatcg caagaaaacg caaataaaaa agtagaatct
    45001 ggaatgacag ttccggattt ttttgatata ataaaataat agaaaacgct atcaaaagaa
    45061 ggagggtgat gtcggtgatg gaacatttgt ttaaattctt acttttagca ccgtactttt
    45121 attttgataa ctggattgag aaggccaaca gaaatagtaa gttttttcca attttttatt
    45181 atttttactg gttttacatc cccttctatt ctctttttag ccttgcttgg acagttgttt
    45241 cagttctgtt tttcaatacc gtcttgagaa atgtgacaga tatcaagtta tggggcattt
    45301 ggtttctttt tattctgcta gctattggta tgaattggtt aacttattcc tgtttcaaag
    45361 aaatgtttcg cttgagacag gaactaggga agtctaaggg tggaaggcat tgatttatat
    45421 cggtttctat ttgtaggagg tggcttatgg tacctctaaa atcatttatt acacgctatt
    45481 ccaaggttta tattggtaat aacatttaat agaacgaaac taaagaaaac aatttaaaag
    45541 aggaggtttg tgatggaata cttgtttaaa atgataatct tattaccctg tttttacttt
    45601 ttcagctgga ttgaaaaggc aaacagagat agtaagattt tcccaatttt ttactatttt
    45661 tactggattt acatcaccct ctatgctctt tttagccttg cttggacagt tttttcagtt
    45721 ctgtttttca atatcgtctt gagaaatttg acagatatca agttatgggg tatttggcta
    45781 ttattactac ttatcgcttt tgctagtgac tggttagact atatctgttt caaaaaaatg
    45841 cttgacttga gacgggaact agggaagtct aaaggcggaa ggcattgatt tatactcttc
    45901 gaaaatctct tcaaaccacg tcaacgtcgc cttggattat atatgtaact gacttcgtca
    45961 gtcttatcta caacctcaaa gcagtgcttt gagcaacttg cggctagttt cctagtttgc
    46021 tctttgattt tcattgagta ttatattact ttctatttgt aggaggtggc ttatgaagat
    46081 tcctctctta actttggcaa ggcataaatt tgtttatgtc ttgcttactt tgctttttct
    46141 tgctttggtt tatcgtgatg ttttgatgac ttatttcttt tttgatattc atgcgcccga
    46201 tctagctaaa ttcgatggac aagcaattaa aaatgactta ttaaaatcag cattagattt
    46261 tcgtattctc cagttcaatc taggttttta tcaatcattt attattccaa tcatcattgt
    46321 tttgctaggt tttcaatata ttgagctgaa aaataaagtt ttacgattga gtattggaag
    46381 agaagtgagc tatcaagggt taaaaagaaa gttgactttg caagttgcaa gtatcccttg
    46441 tttgatatat ttagtgactg tgctgataat tgcaattata acctatttct ttgggacttt
    46501 ttctcctctt gaatggaatt ctctattttc tgatgggagt ggtttacaaa gactcctaga
    46561 tggagagata aaaagctatt tgttctttac ttgtgtccta ctaatcggta ttttcatcaa
    46621 tgcaatctat tttttacaaa tagttgatta tgtggggaat gtgactcgtt cggcaatcac
    46681 ctatttgatg tttctttggc ttggttctat gctgctctat agtgccttgc cttactatat
    46741 ggttcctatg acgagtttga tgcaagctag ctatggggat gtaagtttga tgaaactctt
    46801 tactccttat atccttcatg ttgtccctta tatggtactt aaaaaatatg aagataatgt
    46861 ttaagaattt taacaatatt tggctaaaga gaaagatcgt tttactactt cgtatagttc
    46921 tgatgatgat tttgataaac tatctattgt caacagcggt tcaaaagcag gatgctgtta
    46981 tctttttcaa gagagaattg atttcaattt tttcctataa tgactattct gaagcgaatt
    47041 tagaaatccc caaactcttg ttaaaccttt cgcttttcat ggtaggatgg ctctctgtca
    47101 ttttacttga aagtgatttg gcagaccatt accatcactt gattcgctat caatcaagct
    47161 cctttttcga ttatacaagg aaacgattgg ttgtcatttc taaatttttt actcaagatt
    47221 tgtttgtctg gtttcttggt ttacttccgc taggaattca tttcaaaaca gtcgcacttt
    47281 tctttttact tgctcagtta atgatgttgt acttactact gtcttatctg atagcactga
    47341 ttagtgcggg cgctggtttt tctttttttc tctatttttt ggcatttgtg ggacaagaat
    47401 ggatgatgga tcatattgta acagtgtatt tagtactctt aagcttatta gttatgttga
    47461 ttgttagtcg cttggaagag aaatttaaga aaggataaac gatgagactt gaaattataa
    47521 atggacagaa aatttatggg aaaagaccta ttttaaatca gttgaatttg gtgtttcaat
    47581 caggaaaaat ttatggactt aaaggtgata atggatctgg caagacggtt cttttaaaga
    47641 tacttgctgg ttatattaag cttgacaaag gaaaagttct tcaagatggt aaagtttacg
    47701 gggtaaaaaa tcattatatt caggatgcag gaattttaat tgaaaaagtc gagtttttat
    47761 ctcatttatc cctgagagaa aatttggaac tgttaaggta tttttcatct aaagttacgg
    47821 aaaaaagaat tgcctattgg attcaatact atgatttaca ggaatttgaa gacgttgaat
    47881 accgtcattt atccttggga acaaagcaaa aaatggcctt gattcaagcc tttatttcct
    47941 ctccttctat actctttctc gatgaaccta tgaatgcttt ggatgagaag agtgtgaggt
    48001 taaccaaaca ggtcatttta tcttacctga aaaaagaaaa tggtctggtt atcctgacgt
    48061 cgcacatatc ggaagatatt tcagaccttt gtacagatgt attagttgtc gaaaatggac
    48121 atatacaata tgtaaaggat atacaatcct aggagatggc ttatggcaca tctaaaatca
    48181 tttattacac gctattccaa ggtttatatt ggtttagttc tgctgatctg gctgtctttc
    48241 ttctttatcc cttgggatag agcgattctg ggggtaagga ttgacatctt catcatgcag
    48301 aaaatcttgc tagcttttgg aattctgtcc attctcatgg ccttgctgtc caagaaagtc
    48361 agtctctttg tttttggact gatttgctgt ctttctcttt ggattaactt atttatcaca
    48421 tttgccattt tgccgatttt tggcaattaa acagtcataa aagtcggaga ggttagcttg
    48481 aaaactaacc tctttttcct tttcaaaatg gggattcttc cttgaaaata atcagtaatt
    48541 gtgctaaaat taaaggaaca ttctaaaata ttcggaattt aaagtaagga aaaacatggc
    48601 taatatttta aaaacaatta tcgaaaatga taaaggagaa atccgtcgtc tggaaaagat
    48661 ggctgacaag gttttcaaat acgaagacca aatggctact ttgactgacg accaactaaa
    48721 agcaaaaaca gttgaattta aggaacgtta tcaaaatgga gaatcactgg attcattgct
    48781 ttacgaagca tttgcggttg tccgtgaagg tgccaaacgt gtcctaggtc tcttccctta
    48841 taaggttcag gtcatggggg ggattgttct tcaccatggt gacgtgccag agatgcgtac
    48901 aggggaaggg aaaaccttga ctgcgaccat gccggtatac ctcaatgccc tttcaggtaa
    48961 aggggttcac gtagttacgg ttaatgaata cctgtcagaa cgtgacgcga ctgagatggg
    49021 tgaattgtac tcttggcttg gtttgtcagt agggattaac ttggctacca aatctccaat
    49081 ggagaaaaaa gaagcctatg agtgtgatat tacttactca actaactcag aaatcggatt
    49141 tgactacctt cgtgacaaca tggtcgttcg cgccgaaaac atggtacaac gtccgcttaa
    49201 ctatgccttg gtcgatgagg ttgactctat cttgattgac gaggctcgta cacctttgat
    49261 tgtatcaggt gccaatgcgg ttgaaaccag tcagttgtat cacatggcag accactatgt
    49321 aaaatctttg aacaaagatg actacatcat cgatgtgcag tctaagacta ttggtttgtc
    49381 tgattcaggg attgacaggg ctgaaagcta cttcaaactt gaaaacctct atgacatcga
    49441 aaacgtggct ttgactcact ttatcgataa cgcccttcgt gccaactaca tcatgcttct
    49501 cgatattgac tatgtggtga gcgaagagca agaaatcttg attgtcgacc aatttacagg
    49561 tcgtaccatg gaaggtcgtc gttattctga tggattgcac caagctattg aagccaaaga
    49621 aggtgtgcca atccaggatg aaaccaagac atctgcctca atcacgtacc aaaacctttt
    49681 ccgtatgtac aagaaattgt ctggtatgac gggtacaggt aagactgagg aagaagaatt
    49741 ccgtgaaatc tacaacattc gtgttattcc aatcccaaca aaccgtcctg ttcaacgtat
    49801 tgaccactca gaccttcttt atgcaagtat cgaatctaag tttaaagcgg ttgtcgaaga
    49861 cgttaaggct cgttaccaaa agggtcaacc tgtcttggtt ggtacagtgg cggttgaaac
    49921 tagtgactac atttctaaga aattggttgc agctggtgtt cctcacgaag tcttgaatgc
    49981 caaaaaccac tatagagaag cccaaatcat catgaatgct ggtcaacgtg gtgccgttac
    50041 catcgcaacc aacatggcgg gtcgtggtac cgacatcaag cttggtgaag gtgttcgtga
    50101 acttggagga ctttgtgtta ttggtacaga acgtcatgaa agtcgtcgta tcgataacca
    50161 gcttcgtgga cgttcaggtc gtcaaggaga tccaggtgag tcacaattct acctatctct
    50221 tgaagatgat ttgatgaaac gttttggttc tgaacgcttg aagggaatct ttgaacgttt
    50281 gaacatgtct gaagaggcga ttgagtctcg catgttgacg cgtcaggttg aagcagctca
    50341 gaaacgtgtc gaaggaaata actacgatac ccgtaaacaa gtccttcaat acgatgatgt
    50401 catgcgtgaa caacgtgaga ttatctatgc tcaacgttac gatgtcatca ctgcagatcg
    50461 tgacttggca cctgaaattc aggctatgat caaacgcacg attgaacgtg tcgttgatgg
    50521 tcatgcgcgt gccaaacaag atgaaaaact agaggcaatt ttgaactttg ctaagtacaa
    50581 cttgcttcct gaagattcta ttacgatgga agacttgtca ggcttgtctg ataaggccat
    50641 caaggaagag cttttccaac gtgccttgaa ggtttacgat agtcaggttt caaaactacg
    50701 cgatgaagaa gcagttaaag aattccaaaa agttttgatt ctacgagtgg tggataacaa
    50761 gtggacagat catatcgatg cccttgatca attgcgtaac gcggttggac ttcgtggcta
    50821 tgctcagaac aaccctgttg ttgagtatca ggcagaaggt ttccgtatgt ttaatgatat
    50881 gattggttcg attgagtttg atgtgacacg cttgatgatg aaagcacaaa ttcatgaaca
    50941 agaaagacca caggcagaac gtcatatcag tacaacagcg actcgcaata tcgctgctca
    51001 ccaagcaagt atgccagaag atttggattt gagccagatt ggacgcaatg aactttgccc
    51061 atgtggttct ggtaagaaat ttaaaaactg tcacggtaaa agacaataaa atgagatagt
    51121 ttagaggcgg atatcttgtg aaaagtaaat ttttactggg tatccgtttg ctttataagg
    51181 agatgagtta tggtatttac agcaaaaagc tctaaaataa atatagaaga agttcgtgcc
    51241 ttgtcaaaat tagaaggtca ggctttggag aggaaatcac agcgcgatca agagctagaa
    51301 gccattatac gtggagaaga tcagcgaatt ctcttggtaa tcgggccatg ctcatctgac
    51361 aacgaagaag ctgtccttga atacgctaag cgtttggcag tcctacaaga agaagtggca
    51421 gatcgtatct ttatggttat gcgtgtttat actgccaaac cccgtaccaa cggagatggc
    51481 tataagggct tgattcacca gcctaacgcg acagaagcgc ctagtcttat caatggaatc
    51541 aaagccgttc gccatcttca ctatcgtgtc atcacagaaa cagggatgac aactgctgat
    51601 gaaatgcttt atcctgaaaa ccttccgctt gtagatgatt tgatttctta catggcagtt
    51661 ggtgcccgtt cagttgaaga ccagcaacac cgctttgtgg caagtggggc aggattttct
    51721 actggtttta aaaatccaac ctctggaaat ctcaatgtca tgtttaatgg gatttatgct
    51781 gctcaaaaca aacaaagttt ccttttctta ggaaaagaag tagaaacaac tgggaacccg
    51841 ctttcacacg ctattcttcg tggtgctctt aatgagtatg gaaaaaatat tcccaactac
    51901 tattatgaca atttaattga taccattgcc caatatgaga aaatgggctt ggaaaatcct
    51961 tttatcatca ttgataccaa tcatgacaat tctggtaagc agtatattga acagatccga
    52021 attgtccgcc agaccttgat taaccgtgct tggaatgaaa aaattaagca gttcgttcgt
    52081 ggttttatga ttgagtctta tctggaagat ggtcgacaaa atgagccaga agtatttggc
    52141 aagtctatca cagacccttg cctgggttgg gataacacag aagctcttgt cagagaaatt
    52201 tacaaaacgt taggagaata agatggcatt tattgaaaaa ggtcaagaaa tcgatatgga
    52261 agtcatcaag gctgaaaccc aattgtctgc ggaagccttg agactcaagg aaagccgtga
    52321 cagggaattg gcagatatta tttcagggga agatgaccgt attctcttgg tgattggtcc
    52381 ttgctcttct gataatgaag aggcggtctt ggaatatgct cgccgtttat ccgccttgca
    52441 aaagaaggta gcggacaaga ttttcatggt catgcgcgtg tatactgcta agcctcgtac
    52501 caatggagac ggctataaag gattggttca ccagccagat acttctaagg ctccaagcct
    52561 gattaacggt ttgcaggctg tgcgccagtt gcattaccgc gtgattacag agactggttt
    52621 gacaacggca gatgagatgc tttatccgtc aaatctgatc ttggtggatg acttggtcag
    52681 ctaccatgcc gttggagctc gttctgtgga agaccaagag caccgctttg tggcttctgg
    52741 gattgatgca ccagtaggga tgaaaaatcc aacctcagga aatttgggtg ttatgtttaa
    52801 cgccatctat gctgctcaaa acaagcaaac cttcctttat catgggcagg aagttgagac
    52861 atcaggtaat cctttggccc atgttatcct ccgtggagca gtcaacgagt atggcaatta
    52921 tatgccgaat tactactatg aaaacctact ccaagccatt gaacgctatg aaaccatggg
    52981 acttgaaaat ccttttatcc tcattgacac caaccatgat aactcaggca agcaatatat
    53041 ggagcagatt cgaattgttc gccagacctt gcagaatcgt gattggaatg agaaaattaa
    53101 aaagacggtt cgaggattta tgattgaatc ttacctagca gatggtcgtc aaaaccaacc
    53161 agagatcttt ggttgctcta ttactgaccc ttgcctaggt tgggaaaata cagaggcctt
    53221 ggtagaagag atttatgtta ccttgacaaa ataagtgaaa aggatggagt tggggaatct
    53281 caactccttt tgatgagaat gatagttgga cacggaattg acatcgaaga attggcttcg
    53341 atagaaagcg cagttacacg acatgaagga tttgctaagc gtgtactgac cgctcaggaa
    53401 atggagcgct tcaccagtct caaaggacgc aggcaaatag aatatttagc tggtcgctgg
    53461 tcggctaagg aggccttttc caaggctatg ggaacgggca ttagcaagct cggttttcag
    53521 gatttggaag tattgaacaa tgaacgtggg gcgccttatt ttagtcaggc accattttca
    53581 ggaaagattt ggctgtctat cagccacacc gatcagtttg tgacagccag tgtcattttg
    53641 gaggaaaatc atgaaagcta gtccacatag accaaccaag gctctgattc atctgggagc
    53701 tattcgacaa aatattcagc aaatgggggc tcatatccct caaggaacgc tcaagttggc
    53761 tgtggttaag gccaatgctt atggtcatgg agctgttgcc gttgccaagg caattcaaga
    53821 tgatgttgat ggcttttgcg tttccaatat cgatgaagcc attgaactca gacaagctgg
    53881 actcagcaag ccaatcctca ttttaggagt ttctgaaatc gaagctgttg ctctagctaa
    53941 agaatatgac ttcaccttga cagtggctgg actggagtgg attcaagcac tcttagataa
    54001 ggaagtggac ctaactggat tgacagtcca cctcaagatt gattcaggga tgggacggat
    54061 tggttttaga gaggcaagtg aggttgagca ggctcaagat ttgctccaac aacacggtgt
    54121 tcgtgttgaa ggaatcttta cccactttgc tactgctgat gaggaatcag atgactattt
    54181 taatgcccag ttagaacggt ttaaaactat tttagctagt atgaaggaag ttccagagct
    54241 ggttcatgct agcaattctg caacgactct ttggcatgta gagactattt tcaatgcggt
    54301 tcgtatggga gatgccatgt atggtctcaa tccaagtgga gcggtcttgg ctttgcctta
    54361 tgatttgata ccggccttga ccttggagtc tgctctggtt catatcaaga cagttccagc
    54421 tggtgcttgc atgggctatg gagcaactta tcaaacggat agcgagcaag tcatcgcgac
    54481 cgtgccaatc gggtatgcag atggatggac aagagacatg caaaatttct ctgtcttggt
    54541 agatggccaa gcttgcccaa ttgtcggcag ggtttcgatg gaccaaatca ctattcgatt
    54601 gcctaagcct tacccactcg gaaccaaggt aaccttgatt ggctccaatg gggataagga
    54661 aatcactgca actcaggtag cgacctaccg cgtaaccatt aactatgagg tggtttgcct
    54721 cctcagcgac cgtattccga gagaatatta ttagaaaaga aaggagtgga gcatgaatct
    54781 acatcaaccc ttgcatgtct tgcctggtgt gggaccaaag tcagcagaaa aatacgccaa
    54841 actaggaatt gaaaacttgc aagatctctt gctctacttt cctttccgtt atgaagactt
    54901 caaaaccaag caggtgctgg aactagaaga cggtgagaag gcagttcttt ctggtcaggt
    54961 agtgactcct gctagtgtcc agtattatgg tttcaagcgc aatcgcctgc gttttagtct
    55021 caagcaggga gaggtcgttt ttgcggtgaa tttctttaac cagccctatc tggctgataa
    55081 aatagagttg ggagcaaccc ttgctgtctt tggaaaatgg gaccgcgcta aggctagtct
    55141 gactgggatg aaggttctgg ctcaggtaga agatgacctc cagcctgtct atcgtctggc
    55201 tcagggaatc agtcaggcca gtctggtcaa ggtcatcaag acggcttttg atcagggact
    55261 ggacctcttg atagaagaaa atctgcccca gtctttacta gacaaataca aactcatgtc
    55321 ccgttgtcag gcagtccgag ctatgcattt tccaaaggat ttggcagaat acaagcaggc
    55381 tcttcgccgt atcaagtttg aggaactctt ttatttccaa atgcagttgc agatgctcaa
    55441 gtctgaaaat agagttcagg gaagtggtct ggttctgaat tggtctcagg aaaaagtgac
    55501 agcagttaaa gcaagtcttc cttttgccct gacccaagct caggaaaaga gtttgcagga
    55561 aattttaact gatatgaagt ccgaccacta catgaatcgt ctcctacaag gagatgtggg
    55621 gagcggaaaa acggtagtcg ctggcttggc catgtttgcg gcagtgacag caggttatca
    55681 ggctgcccta atggtaccaa cagaaatcct cgcagagcaa cactttgaga gtttacagaa
    55741 cctttttccc aatttgaaac tggctctctt gacaggttcc ttgaaagctg cagaaaagag
    55801 agaggtcttg gagaccattg ccaagggtga ggctgatttg attataggaa ctcacgctct
    55861 gatacaagat ggggtggagt atgctcgtct tggtttgatt attatcgatg agcagcaccg
    55921 ttttggtgtg gggcaaaggc gtattttacg ggaaaaaggc gacaatccag atgtcctcat
    55981 gatgacggcg actcccattc cacggacgct tgccatcaca gcctttggag atatggatgt
    56041 ttccattatc gaccagatgc cagcaggtcg gaagcctatt gtgacgcgct ggatcaaaca
    56101 tgagcaacta cctcaggtct tgacttggtt agagggggaa attcaaaaag gttcccaagc
    56161 ctatgtcatc tctcctttga ttgaagaatc agaagctcta gatttgaaaa atgccattgc
    56221 cttatcagag gagttgacga ctcattttgc aggcaaggca gaggtggctc ttctacatgg
    56281 taggatgaag agtgacgaaa aagaccagat catgcaggat ttcaaggaga gaaagacgga
    56341 tattctggtt tcgacgacgg ttattgaggt tggggtcaat gttcccaatg cgactgtcat
    56401 gattatcatg gatgccgatc gcttcggtct cagtcaactt caccagctta gaggtcgtgt
    56461 cggtcggggg gacaagcagt cctacgctgt tctcgttgcc aatcccaaga cggattctgg
    56521 gaaagaccgc atgcgcatca tgacagaaac gaccaatgga tttgtccttg cggaggaaga
    56581 tttgaaaatg cgtggttctg gtgagatttt tggaaccaga cagtcaggac ttccagagtt
    56641 ccaagtggct gatattatcg aagattttcc gattttagaa gaagcaagaa aggttgctag
    56701 ctacattagt tctatagaaa cttggcaaga agatccagag tggcgcatga ttgcccttca
    56761 tctggaaaag aaagaacatc tggattaagc tttctctaag gaaaacttaa gctagttttt
    56821 agatttggct cttatactaa agtcatcaaa aagaaacgag gactctcata tgacagtaac
    56881 gattaaagta aattaccaaa ccactttcca aaagaaggaa gca
//
LOCUS       DAVKMU010000007        52882 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKMU010000007 DAVKMU010000000
VERSION     DAVKMU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 52882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.007"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            265..501
                     /locus_tag="VW343_000492"
     CDS             265..501
                     /locus_tag="VW343_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9755100.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            732..2018
                     /locus_tag="VW343_000493"
     CDS             732..2018
                     /locus_tag="VW343_000493"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9755101.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            2219..2686
                     /locus_tag="VW343_000494"
     CDS             2219..2686
                     /locus_tag="VW343_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9755102.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            2719..2812
                     /gene="ffs"
                     /locus_tag="VW343_000495"
     ncRNA           2719..2812
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW343_000495"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2872..3315
                     /locus_tag="VW343_000496"
     CDS             2872..3315
                     /locus_tag="VW343_000496"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9755103.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            3317..3832
                     /locus_tag="VW343_000497"
     CDS             3317..3832
                     /locus_tag="VW343_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9755104.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     TPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            3846..5207
                     /gene="radA"
                     /locus_tag="VW343_000498"
     CDS             3846..5207
                     /gene="radA"
                     /locus_tag="VW343_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9755105.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYISGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            5280..5777
                     /locus_tag="VW343_000499"
     CDS             5280..5777
                     /locus_tag="VW343_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9755106.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            5798..6142
                     /locus_tag="VW343_000500"
     CDS             5798..6142
                     /locus_tag="VW343_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755107.1"
                     /translation="MYEEKQYFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDFMNCTPKV
                     RQKKSNFWGVLL"
     gene            6139..7490
                     /locus_tag="VW343_000501"
     CDS             join(6139..6580,6580..7490)
                     /locus_tag="VW343_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9755108.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNSMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
     gene            7496..8056
                     /locus_tag="VW343_000502"
     CDS             7496..8056
                     /locus_tag="VW343_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9755109.1"
                     /translation="MSNFLGSVHLLGVLFPDSTFLNAFESAIVAPLVEESLKLLPLVF
                     VLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIHTDLPEGFDFTISRILERIISGIAS
                     HWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLALGTHFLFNSPFVELETELPLAI
                     PVVTAIALYGFYHAYCFVEKHNELMT"
     gene            8201..9169
                     /locus_tag="VW343_000503"
     CDS             8201..9169
                     /locus_tag="VW343_000503"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9755110.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            9287..9640
                     /locus_tag="VW343_000504"
     CDS             9287..9640
                     /locus_tag="VW343_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9755111.1"
                     /translation="MTVRHPGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAQNVTIL
                     EGSNIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            9855..9971
                     /locus_tag="VW343_000505"
     CDS             9855..9971
                     /locus_tag="VW343_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9755112.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            9964..10214
                     /locus_tag="VW343_000506"
                     /pseudo
     CDS             9964..10214
                     /locus_tag="VW343_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866636.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10823..13456
                     /gene="polA"
                     /locus_tag="VW343_000507"
     CDS             10823..13456
                     /gene="polA"
                     /locus_tag="VW343_000507"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9755113.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVGTEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            13541..13978
                     /locus_tag="VW343_000508"
     CDS             13541..13978
                     /locus_tag="VW343_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9755114.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(14214..15224)
                     /locus_tag="VW343_000509"
     CDS             complement(14214..15224)
                     /locus_tag="VW343_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9755115.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            15373..16542
                     /locus_tag="VW343_000510"
     CDS             15373..16542
                     /locus_tag="VW343_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9755116.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEVFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            16539..17309
                     /gene="recO"
                     /locus_tag="VW343_000511"
     CDS             16539..17309
                     /gene="recO"
                     /locus_tag="VW343_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9755117.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKCMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            17306..18298
                     /gene="plsX"
                     /locus_tag="VW343_000512"
     CDS             17306..18298
                     /gene="plsX"
                     /locus_tag="VW343_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9755118.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            18304..18537
                     /locus_tag="VW343_000513"
     CDS             18304..18537
                     /locus_tag="VW343_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9755119.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            18583..18873
                     /locus_tag="VW343_000514"
                     /pseudo
     CDS             18583..18873
                     /locus_tag="VW343_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            19076..19306
                     /gene="blpU"
                     /locus_tag="VW343_000515"
     CDS             19076..19306
                     /gene="blpU"
                     /locus_tag="VW343_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9755120.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            20021..22174
                     /locus_tag="VW343_000516"
     CDS             20021..22174
                     /locus_tag="VW343_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9755121.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            22187..23536
                     /locus_tag="VW343_000517"
     CDS             22187..23536
                     /locus_tag="VW343_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9755122.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKYVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            23706..24413
                     /locus_tag="VW343_000518"
     CDS             23706..24413
                     /locus_tag="VW343_000518"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9755123.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNVAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            24415..24558
                     /locus_tag="VW343_000519"
     CDS             24415..24558
                     /locus_tag="VW343_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9755124.1"
                     /translation="MFATENLRLSTKRTQAEKVPQTFSLCRELGELTDVYEIVWEKLQ
                     GLK"
     gene            24615..28340
                     /locus_tag="VW343_000520"
     CDS             24615..28340
                     /locus_tag="VW343_000520"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9755125.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            28433..29875
                     /locus_tag="VW343_000521"
     CDS             28433..29875
                     /locus_tag="VW343_000521"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9755126.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGVEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            30094..31116
                     /locus_tag="VW343_000522"
     CDS             30094..31116
                     /locus_tag="VW343_000522"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9755127.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIAGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            31113..31658
                     /gene="purN"
                     /locus_tag="VW343_000523"
     CDS             31113..31658
                     /gene="purN"
                     /locus_tag="VW343_000523"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9755128.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            31742..32251
                     /locus_tag="VW343_000524"
     CDS             31742..32251
                     /locus_tag="VW343_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9755129.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            32276..33823
                     /gene="purH"
                     /locus_tag="VW343_000525"
     CDS             32276..33823
                     /gene="purH"
                     /locus_tag="VW343_000525"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9755130.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            33945..35207
                     /gene="purD"
                     /locus_tag="VW343_000526"
     CDS             33945..35207
                     /gene="purD"
                     /locus_tag="VW343_000526"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9755131.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            35610..36098
                     /gene="purE"
                     /locus_tag="VW343_000527"
     CDS             35610..36098
                     /gene="purE"
                     /locus_tag="VW343_000527"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9755132.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            36085..37176
                     /gene="purK"
                     /locus_tag="VW343_000528"
     CDS             36085..37176
                     /gene="purK"
                     /locus_tag="VW343_000528"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9755133.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            37186..37413
                     /locus_tag="VW343_000529"
     CDS             37186..37413
                     /locus_tag="VW343_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9755134.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            37476..38774
                     /gene="purB"
                     /locus_tag="VW343_000530"
     CDS             37476..38774
                     /gene="purB"
                     /locus_tag="VW343_000530"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9755135.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(38829..42767)
                     /gene="strH"
                     /locus_tag="VW343_000531"
     CDS             complement(38829..42767)
                     /gene="strH"
                     /locus_tag="VW343_000531"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9755136.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLETLDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(43082..43798)
                     /locus_tag="VW343_000532"
     CDS             complement(43082..43798)
                     /locus_tag="VW343_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9755137.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            44146..45933
                     /locus_tag="VW343_000533"
     CDS             44146..45933
                     /locus_tag="VW343_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9755138.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            45930..46406
                     /locus_tag="VW343_000534"
     CDS             45930..46406
                     /locus_tag="VW343_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9755139.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            46434..47339
                     /locus_tag="VW343_000535"
     CDS             46434..47339
                     /locus_tag="VW343_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9755140.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            47326..48141
                     /locus_tag="VW343_000536"
     CDS             47326..48141
                     /locus_tag="VW343_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9755141.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            48148..48552
                     /locus_tag="VW343_000537"
     CDS             48148..48552
                     /locus_tag="VW343_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9755142.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            48805..49971
                     /locus_tag="VW343_000538"
     CDS             48805..49971
                     /locus_tag="VW343_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9755143.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCATDGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            50087..51124
                     /locus_tag="VW343_000539"
     CDS             50087..51124
                     /locus_tag="VW343_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9755144.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            51332..52186
                     /locus_tag="VW343_000540"
     CDS             51332..52186
                     /locus_tag="VW343_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9755145.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWNIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            52336..52593
                     /locus_tag="VW343_000541"
     CDS             52336..52593
                     /locus_tag="VW343_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9755146.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT        14930 a         9826 c        12570 g        15556 t
ORIGIN      
        1 tgaatttttt tatactagaa aatcagaacc ataataccta tataaaaata ttatagtttt
       61 aataggattt acccaaaagt tttaaggcgg tctttttaga actttaattg tttgaaattt
      121 aggtagcaaa tttgtttcta ttttgtcaac ttttcctatt tttatcttgt tgaggctggt
      181 attttaacaa ttcaggaatt gatagtgaat gtgtaaaatt ttttgttaga ataagtttat
      241 aaaaaagaaa aggagtattt gattatgtta caaaaaattt atgagcagat ggctaatttc
      301 tatgatagta ttgaagaaga gtatggtcct acatttggtg ataattttga ctgggaacat
      361 gttcatttta aatttttaat ttattattta gtgagatatg gcattggttg tcgtagggat
      421 tttatcgttt accattatcg tgttgcttat cgtttgtatc ttgaaaaatt ggtaatgaat
      481 cgggggttta tttcttgttg aggtaatttt agtaaatttc cgaactaatt tactctttta
      541 tggaaagata atagtaaata gctagtaatt tttctaaatc attttttaat agttggaaat
      601 agcaaatctt tctattgttt cttcttgata aaaaggcgat tttttattat aataaattgt
      661 aagatataat tgcaggtgag agtcctgcca tgtatgtgag aaaggaagag cctgagggct
      721 cagacaaaat tatgacttca gttgttgttg taggtaccca atggggtgat gaaggtaaag
      781 ggaagattac agacttcctt tcagcgaatg cagaagtaat tgcacgttac caaggtggtg
      841 ataatgctgg tcacacgatt gtgattgacg gtaagaaatt taagttgcac ttgattccat
      901 ctgggatttt cttccctgaa aaaatatctg tcattgggaa tggtatggtt gtaaatccta
      961 aatctcttgt aaaagagttg agctatcttc atgaggaagg tgtaacaact gataacttgc
     1021 gtatttctga tcgtgcgcat gttattttgc cttatcatat cgagttggat cgcttgcaag
     1081 aagaagctaa gggcgacaat aagattggta cgacaattaa gggaattggt ccagcttata
     1141 tggacaaggc tgctcgtgtt ggaattcgta ttgcagatct tttagataaa gatattttcc
     1201 gtgagcgttt agaacgtaac cttgctgaaa agaatcgtct ttttgaaaaa ttgtatgaca
     1261 gtaaagcgat tgttttcgat gatatttttg aagaatatta cgaatatggt caacaaatca
     1321 agaaatacgt gatagataca tctgttatct tgaatgatgc gcttgataat ggcaaacgtg
     1381 tgctttttga aggtgcacaa ggtgttatgc tagatatcga ccaaggtact tatccatttg
     1441 ttacgtcatc aaaccctgta gctggtggtg tgacaattgg ttctggtgtc ggtccaagca
     1501 agattgacaa ggttgtaggt gtatgtaaag cttatacgag tcgtgtagga gatggtcctt
     1561 tcccaactga gttgtttgat gaagtgggag aacgtatccg tgaagtgggt catgaatatg
     1621 gtacaacaac tggtcgtcca cgtcgtgtag gttggtttga ctcagttgtg atgcgtcata
     1681 gccgtcgtgt ttctggtatt actaaccttt ctttgaactc tattgatgtt ttgagcggtt
     1741 tggatactgt gaaaatctgt gtggcctatg atcttgacgg tcaacgtatt gactactatc
     1801 cagctagtct tgagcaattg aaacgttgca agcctatcta tgaagagttg ccaggttggt
     1861 cagaagatat taccggagtt cgcaatttgg aagatcttcc tgagaatgcg cgtaactatg
     1921 ttcgtcgtgt gagtgaattg gttggcgttc gtatttctac tttctcagta ggtcctggtc
     1981 gtgaacaaac aaatatttta gaaagtgttt ggtcctaaga gatttttaag atttgtttaa
     2041 gataggtcgg gtatactata gacggttaca agaagacctc ctaacttgtt gtaacaaata
     2101 tcctaaactt ttctttttca taataatctc cctatagagt caccgcattc ggtggcttct
     2161 tttgtgttgg gattcatgat ataataataa aatcgataag taggaaaaga gaaaagagat
     2221 gtattatacg cttgaagaaa aagaagtctt tatgagggag gctttgagag aggctgagat
     2281 tgctcttgaa cacgatgaaa ttccaattgg ttgtgtgatt gtcaaagatg gggaaatcat
     2341 tggtcgtggg cataatgcgc gtgaggaatt acagcgagcg gttatgcatg cggaaattat
     2401 ggctatagag gatgcgaact tgagtgagga gagctggcgc ttgctggatt gcacactttt
     2461 tgtgaccatt gaaccttgtg tcatgtgtag tggagcgatt gggcttgccc gcattccaaa
     2521 tgtggtctat ggggctaaaa accagaaatt tggcgctgct gggagtttgt acaatatctt
     2581 gacagatgag cgtctcaatc atcgtgtgga ggttgaaata ggaattttgg aagatgaatg
     2641 cgcagctatc atgcaggatt tttttagaaa tagacggaaa aaataatttt gcttttaaaa
     2701 tgaataggaa tgtgatataa taaatagtgg agcaacagtt ctgcgtgaag cgggtcaggg
     2761 gaggaatcca gcagccctaa gcgatttgaa ttgtgtgctc ttttttcgtg ctttttccga
     2821 ataaataaga tagaataatc tagaataaat gataatagaa aagagaaaat tatgaaaatt
     2881 cgtggttttg aattggtttc gagttttaca gatgaaaatt tattgcccaa gcgtgagaca
     2941 gcgcatgcgg ctggttacga cttaaaggtt gctgtgcgta cagttgttgc gccaggagag
     3001 attgtcttgg ttccgacagg ggttaaggct tatatgcagc cgactgaggt tctctacctc
     3061 tatgatcgtt cttcaaatcc tcgtaagaag ggcttggttt taattaactc agttggggtc
     3121 attgatgggg attattatgg aaatcctgga aatgaagggc atatttttgc gcagatgaag
     3181 aatatcacag accaagaggt tgttcttgaa gttggggaac gtattgtcca ggctgttttt
     3241 gctactttct taattgcaga tggagatgca gctgatggcg ttcgaactgg tggatttgga
     3301 tcgacagggc actagaatga agattatctt tgtacgtcat ggggagccag attaccgtga
     3361 gttagaggag cgttcttata taggatttgg gatagatttg gcacccttgt ctgagatggg
     3421 acggcagcaa gtccagaaat tgagcaaaac tcctttactc tcgtcagctg aaataatcgt
     3481 atcttctgca gtcacaagag ctttagaaac ggcttcgtat gtggtctgtg ctacgggtct
     3541 tcctttaaga gtagagcctt tattacatga atggcaggtc tataaaacag gaatagaaaa
     3601 ctttgaaaca gctagaagac tgtttttaga aaacaagggg gagttgcttc ctaatagtcc
     3661 tattcaatat gagacagcta cggaaatgaa gtctcggttt ctagaatgta tgtctaagta
     3721 tcgagaacat cagactgtgg tagttgttgc tcatcgaatg ctcatgcgcc agtttgtgcc
     3781 aaatgagaag attgattttt gccaagtgat tgagtgtgag ttagagatat agaaagaggt
     3841 ttgtcatcgc aaagaaaaaa gcgacatttg tatgtcaaaa ttgtgggtat aattccccta
     3901 aatatctggg acgttgcccc aactgtgggt cttggtcttc ttttgtggaa gaggttgagg
     3961 ttgccgaggt caagaatgcg cgtgtgtcct tgacaggtga gaaaaccaag cccatgaaac
     4021 tagctgaggt gacttccatc aatgtcaatc gaaccaagac ggagatggag gaattcaacc
     4081 gtgtgcttgg aggcggagtg gtaccaggaa gtctcgtcct catcggtggg gatcctggga
     4141 ttgggaaatc aactcttctc ctacaagtct caacccagtt gtcccaagtg gggacagttc
     4201 tctatatcag tggggaggag tctgcccagc agattaaact acgtgcagag cgcttaggtg
     4261 atattgatag tgagttttat ctctatgcag agaccaatat gcagagtgtt cgtgcagaag
     4321 tggagcgtat ccagccagac tttcttatta ttgattccat ccagaccatc atgtctcctg
     4381 agatttcagg ggtgcagggg tctgtttctc aggtacgtga agtgaccgct gaactcatgc
     4441 agttggccaa gaccaataac attgccatct ttatcgtagg tcatgtgacc aaagaaggaa
     4501 ccttggctgg gcctcgtatg ttggagcata tggtggatac ggtgctttac tttgaagggg
     4561 agcgtcacca cacctttcgt attttgagag cggtcaaaaa tcgttttggt tccactaatg
     4621 agattgggat ttttgagatg cagtcgggcg gcttggttga ggtactcaat ccgagtcaag
     4681 ttttcctaga agagcgtttg gatggggcaa ctggttcctc catcgttgta accatggaag
     4741 ggacgcgtcc gattttggcg gaggttcagg ctttggtaac accgaccatg tttggaaatg
     4801 ccaagcgtac tacgacagga cttgatttta accgtgctag cttgattatg gctgttttgg
     4861 aaaaacgggc agggcttctc ttgcaaaatc aggatgccta tctcaaatct gctggtggtg
     4921 ttaaattgga tgaacctgcc attgacttgg ctgttgcagt tgctattgct tcgagctaca
     4981 aagacaagcc aactaatcct caggaatgtt ttgtcggaga actgggcttg acaggagaga
     5041 ttcggcgcgt gaatcgtatt gagcaacgca tcaacgaagc tgctaaactg ggctttacta
     5101 agatttatgt acctaagaat tccttgacag gaatcactct gcctaaggaa attcaggtca
     5161 ttggcgtgac aacgattcag gaagttttga aaaaggtctt tgcataatcc gtgacaaatt
     5221 ctcttaaaaa tgataagata ggagaaatat ttgactatca aattttcaag gagggaatcg
     5281 tgtcgtattt tgaacagttt atgcaagcta atcaggctta tgttgcccta catgggcagt
     5341 taaatctgcc acttaaaccc aaaacaagag tagctattgt gacctgtatg gactcacgtt
     5401 tgcacgttgc gcaagctcta ggtttggcac ttggggatgc tcatatcttg cggaatgcag
     5461 gtggtcgagt gactgaagac atgattcgtt cgctagttat ttcccagcaa caaatgggga
     5521 caagagagat tgtggtattg caccatacag actgtggtgc tcagaccttt gaaaatgaac
     5581 cttttcagga gtatttaaaa gaggaattag gtgtagatgt gtcagaccag gacttcttgc
     5641 ccttccaaga tatagaagag agtgtacgcg aggatatgca actgcttatc gagtctcccc
     5701 taataccaga cgatgtcatt atctctggtg ctatttacaa tgttgataca ggaagtatga
     5761 cagtcgtaga attataaata cttcatttag aaagaaagtg tatgaagaaa agcagtattt
     5821 tatttttatt ttattgctat gtattggttt acagtatgaa accatctact atacggacgg
     5881 ttcgaggtca ggtgcggaat atggactaat gggagtttct atctttctag ctctctttta
     5941 catgattccg gctctttatt ttctcttccg tattgggaaa aaatgggaat tgccaaagaa
     6001 ggttttgatt ctgtctttat tgggagggat gttcctttca ggctggttgt ctagttttgc
     6061 taatacttat atccatgatt ttatgaactg caccccaaaa gttagacaga aaaaatctaa
     6121 cttttggggt gttttattat gaaattgagt tatgaagata aagttcagat ctatgaacta
     6181 agaaagcaag gacaaagctt caaacagctt tcaaaaagat ttggtgtgga tgtttctggt
     6241 ctaaagtaca tggtgaaatt aattgaccgt tatggcatag aaatcgttaa aaaagggaag
     6301 aatcgtcact attcttctaa attgaaacaa gaaatgatgg ataaagcctt actggaaggt
     6361 tgttcacaaa gaagtattag tcttgattat gccctcccaa accaaggaat gctttcattt
     6421 tggccggcac aatacaagaa aaacgggtat actattgttg agaaaacaag agggagacca
     6481 gctaaaatgg gacgtaaacg gaagaagact tgggaagaaa tgacagaact agaacgactc
     6541 caggaggaga atgaacgctt acggactgag gtggcctacc taaaaaagtt aaaagagtta
     6601 gaggaaaggg acgaagccct agagcgagaa aggcagagac agttagagaa atggtttcag
     6661 gaggatttcg actagatttt cttcttgaaa cagcacgttt agctcgctcg acttactatt
     6721 atcagttgaa acaactggat ggggttgata aagataaaga gattaaaacc gaaattcagg
     6781 gcattgataa tgaacataaa ggcaactatg gctatcgtcg aatccattta gaactaagga
     6841 atcgtggttt tgtggtcaat cataagaaag ttcaacggtt gatgagaatt cttggtttaa
     6901 cggctcgaat tcgccgtaaa cggaagtatt cttcctacca aggagagatt ggcaagaaag
     6961 cagagaatct cattcaacgc cagttcgaag catcgaggcc aatggaaaag tgctatacag
     7021 atgtgacaga gtttgccatt ccaaatagca cacagaaact ctatttatca ccagttttag
     7081 atggttttaa cagtgaaatc attgcttatc atctttctac ttcgccaaac ttagaacaag
     7141 tgaagagtat gttagaacag gcctttacag agaaatacta tgagaatacg attctccata
     7201 gtgaccaagg ctggcaatac caacacgatt cttatcatcg gttcctagag agtaagggaa
     7261 ttcaagcatc catgtcacgc aagggcaaca gcccagacaa cagcatgatg gaatctttct
     7321 ttggcatttt aaaatccgaa atgttttatg gctatgagaa gaactttaga tctttagaaa
     7381 accttgaaca agctattgtg gactacattg attactacaa caacaaacga attaaggtaa
     7441 aactaaaagg acttagttct gtgcagtaca gaactaaatc ctttggataa attatttgtc
     7501 taactttttg gggtcagtac atttactggg ggttcttttc ccagatagta catttttaaa
     7561 tgcctttgaa agtgctattg tggctccttt ggtagaagaa tccttgaaat tattgccact
     7621 tgtttttgtt ttggctttga ttcctgtgcg aaaattaaaa tctttgtttt tacttggaat
     7681 tgcttccggt ttgggattcc aaatgattga ggatattggt tatattcata cggatttgcc
     7741 agagggcttt gactttacta tttcgcgaat tttagagcgt atcatctcag gaattgcctc
     7801 tcactggact ttttcaggtc tagctgtagt aggtgtttac ttgctttaca gagcctataa
     7861 aggacagaag gttggcaaga aacagggcct tatttttcta ggtttagcct tgggaactca
     7921 cttcttgttt aactctcctt ttgtggagtt ggaaacagag ttgcctttag cgattccagt
     7981 ggttacggct attgctctct atggttttta tcatgcttat tgctttgttg agaaacacaa
     8041 tgagttgatg acctagaata tttttcaaaa gaatgatgca agggtacaaa tatggtgccc
     8101 ttcttttatt tttgattgaa aaatagtgca aaaagcgcta caatggtaga tggaaaatct
     8161 tgtgaaaagc acaagttata catatatacc ggaggaaatc atgtcttttt ctgatttaaa
     8221 gctatttgcc ctttcttcta ataaagaatt ggcagaacgt gtggcgcagg agattgggat
     8281 agagttgggg aaatcaagtg ttcgccaatt ttcagatgga gagattcagg tcaacattga
     8341 agaatcaatc cgtgggaaac acgtctttat cctacaatca actagttcgc ctgtaaatga
     8401 caatctgctt gaaattttga ttatggtaga tgctttgaag cgtgcgagtg cagaatctgt
     8461 caatgttgtc atgccttact atgggtatgc acgtcaggat agaaaggcga gagcgcgtga
     8521 gccaatcact tcaaaacttg tcgcaaatat gcttgaagta gctggagtgg atcgtttatt
     8581 gaccatcgac ttgcatgctg cgcaaattca aggattcttt gatattcctg tggatcattt
     8641 gatgggtgct cctctgattg cagattattt tgagcgtcgt ggtatggttg gttctgacta
     8701 tgtggttgtc agcccggacc atggaggggt gactcgtgct cgtaagttgg cagaattttt
     8761 gaaaacttct atcgctatta ttgataaacg tcgtagcgtt gataagatga atactagtga
     8821 agttatgaac atcatcggta aggttgaagg caagacttgt atcttgattg atgatatgat
     8881 tgataccgct ggaacgattt gtcatgcggc agatgctctt gcggaagctg gtgctgttga
     8941 agtctatgca agctgtacgc acccagttct ttctggtcct gctacggaca atatccaaaa
     9001 atcagctatt aagaaattgg ttgttttgga taccatctat ctgccagaag agcgtttgat
     9061 tgataagatt gagcagattt caatcgctca tctcctaggg gatgctatcg tacgtattca
     9121 tgaaaaacga ccactttctc cacttttcga tattgagaaa aaaatttaat gaccaagcct
     9181 gagatgattc tcaggttttt tcgtcttttt tccgaataaa tagatagtag cggtgaatct
     9241 agtaaagcta gatttaaaac tgtgatataa taaaaagaga agaaatatga ctgtacgtca
     9301 tccgggcatt agcccaacca atgacttggt agctaagaaa atctttagca atccagaaat
     9361 tacttgtcaa tttattcgcg atatgctgga tttgccagcc caaaatgtga ccattttgga
     9421 gggtagcaat attcacgtat tactctccat gccttactcg gtgcaggatt tttataccag
     9481 tatagacgtc ttggcggagt tggataacgg tactcaagta attattgaga ttcaagtcca
     9541 tcatcagaat tttttcatca atcacttgtg ggcttacctg tgcagtcagg ttaatcaaaa
     9601 tcttgaaaaa attcgtcagc gagaaggtga tactcactag atctacaaac acatcgctcc
     9661 tgtttacgtc attgctatcg tggatagcaa ttatttctca gatggcctgg cttttcatag
     9721 cgttagtatg cgcgaagaca caacaggtga ggtattggcg attaccaaca atggacagga
     9781 aaaccatctg gttaagatgg cattcttgga attaaaaaat acagagaaac cagcaaagac
     9841 aaggttcgca agccatggtt ggagtttttc ggcaacaagc cctttaccca gcaaccgcaa
     9901 cgagccatta cccaagcaaa tcaactgctg gactacaaga gctggtccga ggaggacagg
     9961 aaaatgttta gtcaactaca tatgcgagaa gaacaagtct tgttagcaca ggactatgcg
    10021 aaggtattga acaaggacta gagcgtggta ttgaacgtgg tcgtgcagag ggttgtgaac
    10081 aaggacgaga agaaggcctt gaagagggat taaaagtagg tttagtaaat ctagtacgtc
    10141 aaggtcttct aacacctgag gttgcgagtg agcaattagg catgaccgtg gctgagtttg
    10201 agtccttgct atgagttcat actttatata gtagaaagtt gatgggatgg caagtttatt
    10261 atcaaaatac ttgtcatttt tgcttgacat ttctatcttt ttataagata tagtaaaatg
    10321 aaataataac aggacaaatc gttcaggaca gtcaaatcga tttctaacaa tgttttagaa
    10381 atcgaggtgt actattctag tttcaatcta ctatagtatt agcgaagtaa gttatgtctc
    10441 gctattcgta cagtttggat agtcgcaaga ttgtctttga aatttcctgt tttaaagaga
    10501 aaaaagcctc tttgacgcta ttttttcatc tttttgaaag ttctataatg aagttagcca
    10561 cccagccctc cttttcctcc ttctatagtg agttgaaata agatgtgaaa caactctatc
    10621 aggaaagtca aattaattta tagaaatatt ttagcagtca aggtgtacta ttatagattc
    10681 aatatactat attatatcac atttttaaat ctaggtttac taaattctct ggctctatct
    10741 atttattcgg aaaaagtttg aaaaatactt gctctagctc ttcccaatgg tattttttga
    10801 ttctttcctt tataatgggt gtatggataa gaaaaaatta ttattgattg atgggtcttc
    10861 tgtagctttt cgggcgtttt ttgcgctgta tcagcagttg gaccgtttta agaatgcggc
    10921 tggtttgcat accaatgcga tttatggttt tcagttgatg ttgagtcatt tgttggagcg
    10981 ggttgagccg agtcatattt tggtggcttt tgatgcggga aagacgacct tccggacaga
    11041 gatgtatgcg gactataagg gtggtcgggc caagactcct gatgagtttc gtgagcaatt
    11101 tcctttcatt cgtgagttgc tggatcatat ggggattcgt cactatgagc tggctcagta
    11161 tgaggcggat gacatcattg ggacgctgga taagctagca gagcaggatg gttttgatat
    11221 taccattgtc agtggggaca aggatttgat tcagctgacg gatgagcata cggtggttga
    11281 aatttccaag aaaggtgtgg ctgagtttga ggcctttacg ccagattacc tcatggaaga
    11341 aatgggcctc acaccagctc agtttatcga tctcaaggcg ctcatgggtg ataagtcgga
    11401 taatatccct ggggtgacca aagtcggtga aaagacgggt attaagctct tgctggagca
    11461 tggttcgctt gaggggattt atgaaaatat tgatggaatg aagacttcta agatgaagga
    11521 aaatctcatc aatgacaagg aacaggcctt tttgtctaaa acactagcga ccattgatac
    11581 caaggcaccg attgcgattg gtttagagga cttggtctat agtggtccag atgttgaaaa
    11641 tcttgggaaa ttttacgatg agatgggctt caaacagctc aagcaggctt taaatgtgtc
    11701 gtcagctgat gtggctgagg gtttggattt tactattgtt gaccaaatca gtcaagatat
    11761 gctgagtgaa gagtctatct tccactttga gctttttggt gagaattacc atacggataa
    11821 tttggttgga tttgcctggt cttgtgggga taagctctat gccacagaca agcttgagct
    11881 gttgcaagac ccgattttca aggatttctt agaaaaaaca tctctgagag tttatgactt
    11941 taagaaggtt aaagttcttt tgcaacgttt tggtgtggat ttgcaggcgc ctgcttttga
    12001 catccgtttg gctaaatacc tcctttcgac tgtggaggac aatgaaattg cgaccatcgc
    12061 tagtctttat ggtcagactt acttggttga tgatgaaact ttctacggta agggtgttaa
    12121 aaaggccatt cctgaacgtg agaaattctt ggaacactta gcttgtaaac ttgctgtttt
    12181 ggtaggaaca gagcctattt tacttgaaaa actcagcgaa aatgggcaat tagagcttct
    12241 ttatgatatg gagcaacctc tggcttttgt tcttgccaag atggaaattg ctgggattgt
    12301 ggtcaagaaa gagaccttgc ttgagatgca ggctgaaaat gagcttgtca ttgaaaaact
    12361 gactcaagag atttacgagc tggctggtga ggagtttaat gtcaactcgc ctaagcagtt
    12421 gggcgtgctt ctctttgaga aattgggact tcctctagaa tacactaaga aaaccaagac
    12481 aggttattcg acagcagtgg atgtgttgga gcgtctcgct cctattgctc cgattgttaa
    12541 gaaaatcctg gattaccgtc aaattgctaa gattcaatct acttatgtaa ttggcttgca
    12601 ggactggatt ttggctgatg gaaagattca tactcgctat atgcaggatt tgacccagac
    12661 cgggcgtttg tctagtgtgg atccaaactt gcaaaatatt cctgctcgat tggaacaagg
    12721 gcgcttgatt cggaaggctt ttgtgccaga gtgggaggat agtgtgctac tcagctctga
    12781 ctattcacag attgaattgc gcgttttggc gcatatttct aaggatgagc acttgattaa
    12841 ggccttccaa gagggggcag atatccatac ttcgacagcc atgcgggtct ttggcattga
    12901 gcgtcctgat aatgtgactg caaatgaccg tcgcaatgcc aaggcagtta actttggagt
    12961 ggtttatggg atttcagact ttggcttgtc taataatttg ggaattagtc gtaaggaagc
    13021 caaagcctac attgatacct actttgaacg ttttccaggt attaaaaact acatggatga
    13081 agtggtgcgg gaggcgcgtg ataagggcta tgtagagacc ctctttaagc gtcgccgtga
    13141 gttgccagat atcaattcgc gcaacttcaa tattcgtggt tttgcggagc gaactgctat
    13201 caactcacct atccagggtt cggcagcaga tattctcaag attgccatga ttcagctgga
    13261 taaagccttg gttgcaggtg gttatcagac taagatgctg ttacaagtgc acgatgaaat
    13321 cgtccttgaa gtgcctaaat ctgaattggt agagatgaaa aaattggtga aacaaaccat
    13381 ggaagaagcc attcaactca gtgttcctct tatcgcagat gagaatgaag gggcaacctg
    13441 gtacgaggct aaataaaaag ggggctagtc ctcctttttt gtagtagaat tctgcaaacc
    13501 ttttcaaaat gtgctatact gatgaaaaag gaggatttct atgagtcaag aatttatcaa
    13561 tccaagtgat ggcgtgattc gtcagtatct cgcaacgagt aaaacccttg ctgtggtggg
    13621 tttgtctgac cgtgaagaaa caactagcaa tcgagtaaca aaggaaatgc aggctcgggg
    13681 ctataaaatc atcccagtca atcccaaggc ggcaggtggc gaaatcttgg gtgaaaaggc
    13741 ttatgctagc ctcgctgaaa ttccttttcc tgtagatatt gtcaatgttt atcgtcgcag
    13801 tgaatttctg cccgatgtgg cgcgtgattt tctcaaagct gatgccaaga ttttttgggc
    13861 acagctagga cttgaaagtt tagaagcgaa agagattttg cgtgatggtg gatgtgatga
    13921 tatcgtgatg aatcgctgta tcaagagaga acatactcgc ttgattgagg aagcataaga
    13981 aaaaggtagc tgatgggcta ccttttgtgt tatactcaat gaaaatcaaa gagcaaacta
    14041 gcaaactagg aaactagccg cgggctgctc aaaacactgt tttgaggttg tagatagaac
    14101 tgacgaagtc agctcaaaac accgttttga ggttgcagat agaactgacg aagtcagtaa
    14161 catatatacg gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga gtattagaaa
    14221 atgccgataa gggtctgcat accaagagtg gtgaggatga tggcaatcca gcagatggct
    14281 ccgagaacaa tggattttcc actggatttg accatagcga ccagattggt tttgagaccg
    14341 atagcactca tggccatgac aatgaggaat ttagagagtt gtttgagagg ggtaaagaaa
    14401 ctactagaca caccgagaga ggtcagaagg gtggttagga gcgatgcaag gataaagtaa
    14461 aggataaaaa gtgggaagac ttttttcagt tgtaggcttt gcttgttttc ttgttggcga
    14521 ctttgccagt aggatagaaa gagcgtgata gggataatgg ccaaagtacg tgtgagttta
    14581 acaatggttg cagactcgag ggtattgctt tggtaaagac tgtcccaagc gctggcggca
    14641 gccgttacag aggaagtgtc gttgaccgca gtccctgcaa agagggcgaa gccttcattg
    14701 gatagatgaa gccaggtgcc gagggttgga aagatgagcg cagccaagac attgaagaaa
    14761 aagataacgg aaatggcttg ggctacttcc ttttccttag catcaataac gggcgctgtc
    14821 gctgcaatgg cagaaccccc acagatagaa gaacctactc caaccaaggt agccagtttt
    14881 gtatccagtg caaagaaacg ctggaagagg taggcaataa tcagagctat tgagatagtg
    14941 gacaggatga cagggagtga agattggcca actgcgaaga cctgcgagat attgagacca
    15001 aaaccaagca agacaacggc atattggagc aacttcttgg aactaaaggt caaaccagca
    15061 tccagttgtt tataggacga gagaaaggga tgtaggagca tgcctatgaa aatggcaaaa
    15121 acgggcgcgc caatcacagg gaagaatcct cctaagtacc aagataggat ggaaatgaga
    15181 aggcaggcca agatacctgc tccatttttt gatagaaatg acataaaaac ctccaaataa
    15241 aatctattac cattatagac ctataaacag gaaaagtaaa atagaaagtg gaaagctatt
    15301 ctctaatgta tttttgcggt cggggggctt ttgtagtata atagagatac gttttgaaag
    15361 taggaggtat ctatggactt aactaagcgc tttaataaac agttagataa aattcaagtt
    15421 tcgttgattc gtcagtttga ccaggctatt tcggagattc ctggggtctt gcgtttgacc
    15481 ttgggggaac ctgattttac aacgccagac catgtcaagg aggcggccaa gcgagcgatt
    15541 gatcagaacc aatcctacta tacagggatg agtggtctgc tgactctacg tcaggcagcc
    15601 agtgactttg ttaaggaaaa gtaccaactg gactatgctc ctgaaaatga aatcttggtt
    15661 acaattgggg cgacagaggc tttatctgcg actttgacgg ctattttgga agagggagac
    15721 aaggtacttt tgccagctcc tgcttatcca ggctatgaac cgattgttaa cttagttggg
    15781 gcagaaattg ttgagattga tacgactgaa aatggttttg tcttgactcc tgagatgttg
    15841 gagaaggcca ttttggagca gggtgataag ctcaaagcgg ttattctcaa ctatccagcc
    15901 aatccgacag gaattaccta cagtcgagag cagttagagg ccttggcagc tgttttacgc
    15961 aagtacgaag tttttgttgt ctgtgatgag gtttactcag aattgaccta cacaggcgaa
    16021 gcccatgtgt ctctaggaac gatgttgaga gaccaggcta ttattatcaa tggtttgtct
    16081 aaatcgcatg ccatgacagg ttggcgtttg gggctgattt tcgctccggc gaccttcaca
    16141 gcccagttaa tcaagagtca ccagtacttg gtcactgccg caaataccat ggcgcaacat
    16201 gctgcggtag aagccttgac ggctggtaaa aatgatgcgg agcccatgaa gaaggaatat
    16261 atccagcgtc gggactatat catcgaaaaa atgactgctc ttggttttga gattatcaaa
    16321 ccagacggtg ccttctatat ttttgctaaa attccagcgg gctacaatca agactccttt
    16381 gcttttctga aggattttgc tcagaagaag gccgttgcct ttatccctgg tgcagccttt
    16441 ggacgttacg gggaaggcta cgtccgccta tcttatgcag ccagcatgga gactatcaaa
    16501 gaagccatga aacgacttga ggagtacatg agagaagcat gattcagtct atcacgagtc
    16561 aaggcttggt gctttacaat cgcaattttc gtgaggatga caagctcgtc aaaattttta
    16621 cagagcaggt tggcaaatgc atgttttttg tcaaacacgc tggtcagtct aagctggcgc
    16681 ctgttattca gcccttggtg ctggcacgat ttctcttgcg aatcaatgat gacggactca
    16741 gttacatcga agactatcat gaggtcatga cttttcccaa gattaatagt gacctctttg
    16801 tcatggccta tgcgacctat gtggcagctc ttgcagatgc tagtttgcag gacaatcagc
    16861 aggatgctcc cttgtttgct tttttgcaaa agactttgga gttgatggaa gcaggcttgg
    16921 attatcaggt tttgaccaat atttttgaaa ttcaaatttt gactcgattt ggaatcagcc
    16981 tcaattttaa tgagtgtgtc ttctgccatc gggttggtca ggcttttgac ttttctttca
    17041 aatatggagc ctgcctctgt ccagagcatt atcatgagga taagagacgt tgtcatctca
    17101 atcccaatat cccctatctg ctcaatcaat ttcaagctat tgattttgag actttggaga
    17161 ccatttcgct caagcctgga atcaagcaag agctacgcca atttatggat caattatatg
    17221 aagagtacgt tgggattcac ctaaaatcaa agaaatttat tgattcccta gcagactggg
    17281 gacaattact aaaagaggaa aagaaatgaa aaaaatcgca gtagatgcca tggggggcga
    17341 ttacgcacct caggccattg ttgagggtgt caatcaagcc ctatctgact tttcagatat
    17401 cgaggttcaa ctttacggag atgaagctaa aatcaagcaa tatctgacag cgacagagcg
    17461 cgtcagcatt atccatacgg atgagaagat tgattcggat gatgaaccta cgagagctat
    17521 taggaataag aaaaatgcca gtatggtatt ggcagccaag gctgtcaaag atggtgaagc
    17581 agacgctgtc ctttcggctg ggaatacagg tgccttgttg gcagcaggat tcttcatcgt
    17641 gggtcgtatc aagaatatcg accgtcctgg actcatgtct accttgccta ccgttgatgg
    17701 aaaaggtttt gacatgctag accttggtgc caatgcagaa aatacagccc agcacctcca
    17761 tcaatatgcg gttctaggtt ccttctatgc taaaaatgtc cgtggcattg cgcaaccacg
    17821 cgttggtttg ctcaacaacg gaacagagag tagcaagggc gacccgcttc gtaaggaaac
    17881 ttatgaatta ctggcggctg atgaaagttt gaactttatc ggaaacgtgg aagcgcgtga
    17941 tttgatgaat ggcgttgcag atgttgttgt ggcagatggt ttcacgggaa acgctgtgct
    18001 caaatccatc gaagggacag ctatgggaat catgggcttg ctcaagacag ctattacagg
    18061 tggtggtctt cgagcgaaac taggtgccct ccttctcaag gacagcctca gaggtttgaa
    18121 aaaacagctc aattattcag atgttggtgg agcggtcttg tttggtgtta aggcacctgt
    18181 tgtcaagact catggctcaa gcgatgccaa ggctgtttat agtacgattc gccagattcg
    18241 taccatgcta gaaacagacg tagttgccca gactgcgcgt gaattttcag gagaataaaa
    18301 gagatgacag aaaaagaaat ttttgaccgt attgtgacca ttatccaaga gcgacaggga
    18361 gaggactttg tcgtgacaga atccttgagt ctgaaagacg atttggatgc ggattctgtt
    18421 gacttgatgg agtttatctt gaccctggaa gatgaattta gtatcgaaat cagcgatgaa
    18481 gaaattgacc aactccaaaa cgtaggagat gtggttaaaa tcattcaagg aaaatagcaa
    18541 tcggagttcc aagtcaacgg aagtagatgg tttttagaaa tgagaaatat cggacaagct
    18601 ggtaaaatct tggctgacag tggttatcaa gggctcatga agatatatcc tcaagcacaa
    18661 actccacgta aatccagcaa actcaagccg ctaacagttg aagataaagc ctgtaatcat
    18721 gcgctatcta aggagagaag caaggttgag aatatctttg ccaaagtaaa aacgtttaaa
    18781 atgttttcaa caacctatcg aaatcatcgt aaacgcttcg gattacgaat gaatttgatt
    18841 gctggtatta tcaatcatga actaggattc tagttttgca ggaagtctaa tagtaaaaaa
    18901 gtgattagaa aacatctttt ttaaaaatag agatgatttt gaaacaaaaa agctaattca
    18961 agacgtttcg atgccaattc aagatttgga tgaaaaaaat taatagatac tgttatacta
    19021 aacttgtcaa gtttgtaaca agacaaatat taaaaataaa aaagaggtat tcgttatgaa
    19081 tacaaaaacg atgtcacaat ttgaaattat ggatactgag atgcttgctt gcgttgaagg
    19141 tggcggatgc aattggggag attttgccaa agcaggtgtt ggaggaggag cagtacgagg
    19201 tcttcagcta ggaattaaaa caagaacatg gcaaggtgca gcaactggtg ctgcgggagg
    19261 agctatactt ggaggtgtgg cctatgcagc gacatgttgg tggtaattat ggattttaaa
    19321 agttttatta ttggtttagt agttggtata tttggtcctt atatggatga tttaattaga
    19381 aaaaaatttt taaagtcttc ggagaagaaa acagaaaaat ctgttaaaaa ataatcaaaa
    19441 ctataaatga tgaatctgaa tcaaaattat tttgcgcatg taaagaggag tcttatagta
    19501 acgagtcaaa aaaggagtaa ctatgaatcg taatttagaa cggtgttatc tattctgact
    19561 aggaatagat cataccagag gtagcttaga aatagcaggg acattagaaa ttgaagtaat
    19621 aaataggatg tcgtaagtgt tactatcaat gatttatttg tttcaagctt gcctagggtg
    19681 acagtaaaaa atcaatttcc tttcaatagc atatttttag tgggcaggac tcttgttctg
    19741 cctatttttt tatccaaaaa gtgcagttgg gagggagata ggctcatttg ggaaggaagt
    19801 ccagtttttg tttagtgatt ggggtaagat agttgttatc agatgagttt atactcttcg
    19861 aaaatcaaat tcaaaccacg tcaacgtcgc cttgccgtat atatgtgact gacttcgtca
    19921 gtcctatcta caacctcaaa acagtgtttt gagcagcctg cggctagttt cctagtttgc
    19981 tctttgattt tcattgagta ttagggaaaa ggagatgaat atgaaatttg ggaaacgtca
    20041 ctatcgtccg caagtggatc agatggactg cggtgtagct tcattagcca tggtttttgg
    20101 ctactatggt agttattatt ttttggctca cttgcgagaa ttggctaaga cgaccatgga
    20161 tgggacgacg gctttgggct tggtcaaggt ggcagaggag attggttttg agacgcgagc
    20221 cattaaggcg gatatgacgc tttttgactt gccggatttg acttttcctt ttgttgccca
    20281 tgtgcttaag gaagggaaat tgctccacta ctatgtggtg actgggcagg ataaggatag
    20341 cattcatatt gccgatccag atcccggggt gaagttgact aaactgccac gtgagcgttt
    20401 tgaggaagaa tggacaggag tgactctttt tatggcacct agtccagact ataagcctta
    20461 taaggaacaa aaaaatggtc tgctctcttt tatccctata ttagtgaagc agcgtggctt
    20521 gattgccaat atcgttttgg caacactctt ggtaaccggg attaacattg tgggttctta
    20581 ttatctgcag tctatcattg atacctatgt gccagatcag atgcgttcga cactagggat
    20641 tatttctatt gggctagtca tcgtctacat cctccagcaa atcttgtctt acgctcagga
    20701 gtatctcttg cttgttttgg ggcaacgctt gtcgattgac gtgattttgt cctatatcaa
    20761 gcatgttttt cacctcccta tgtccttctt tgcgacacgc aggacagggg agatcgtgtc
    20821 tcgttttaca gatgctaaca gtatcatcga tgcgctggct tcgaccatcc tttcgatttt
    20881 cctagatgtg tcaacggttg tcattatttc ccttgttcta ttttcacaaa ataccaatct
    20941 ctttttcatg actttattgg cgcttcctat ctacacagtg attatctttg cctttatgaa
    21001 gccgtttgaa aagatgaatc gggataccat ggaagccaat gcggttctgt cttcttctat
    21061 cattgaggac atcaacggta ttgagactat caagtccttg accagtgaaa gtcagcgtta
    21121 ccaaaaaatt gacaaggaat ttgtggatta tctgaagaaa tcctttacct atagtcgagc
    21181 agagagtcag caaaaggctc tgaaaaaggt tgcccatctc ttgcttaatg tcggcattct
    21241 ctggatgggg gctgttctgg tcatggatgg caagatgagt ttggggcagt tgattaccta
    21301 taataccttg ctggtttact ttaccaatcc tttggaaaat atcatcaatc tgcaaaccaa
    21361 gcttcagaca gcgcaggttg ccaataaccg tctaaatgaa gtgtatctag tagcttctga
    21421 gtttgaggag aagaaaacag ttgaggattt gagcttgatg aagggagata tgaccttcaa
    21481 gcaggttcat tacaagtatg gctatggtcg agacgtcttg tcggatatca atttaaccgt
    21541 tccccaaggg tctaaggtgg cttttgtggg gatttcaggg tcaggtaaga cgactttggc
    21601 caagatgatg gttaattttt acgacccaag tcaaggggag attagtctgg gtggtgtcaa
    21661 tctcaatcag attgataaaa aagccctgcg ccagtacatc aactatctgc ctcaacagcc
    21721 ctatgtcttt aacggaacga ttttggagaa tcttcttttg ggagccaagg aggggacgac
    21781 acaggaagat atcttacggg cggtcgaatt ggcagagatt cgagaggata tcgagcgcat
    21841 gccactgaat tatcagacag aattgacttc ggatggggca gggatttcag gtggtcaacg
    21901 tcagagaatc gctttggcgc gtgctctctt gacagatgcg ccggtcttga ttttggatga
    21961 ggcgactagc agtttggata ttttgacaga gaagcggatt gtcgataatc tcatggcttt
    22021 ggacaagacc ttgattttca ttgctcaccg cttgactatt gctgagcgga cagagaaggt
    22081 agttgtcttg gatcagggca agattgtcga agaaggaaag catgctgatt tgcttgcaca
    22141 gggtggcttt tacgcccatt tggtcaatag ctagaaagag gagaggatga aaccagaatt
    22201 tttagaaagt gcggagtttt ataatcgtcg ttaccataat ttttccagta gtgtgattgt
    22261 acccatggcc cttctgcttg tgtttttact tggctttgca actgttgcag agaaggagat
    22321 gagtttgtcc actagagcta ctgtcgaacc tagtcgtatc cttgcaaata tccagtcaac
    22381 tagcaacaat cgtattcttg tcaatcattt ggaagaaaat aagctggtta agaaggggga
    22441 tcttttggtt caataccaag aaggggcaga gggtgtccaa gcggagtcct atgccagtca
    22501 gttggacatg ctaaaggatc aaaaaaagca attggagtat ctgcaaaaga gcctgcaaga
    22561 aggggagaac cactttccag aggaggataa gtttggctac caagccacct ttcgcgacta
    22621 catcagtcaa gcaggcagtc ttagggctag tacatcgcaa caaaatgaga ccatcgcgtc
    22681 ccagaatgca gcagctagcc aaacccaagc cgaaatcggc aacctcatca gtcaaacaga
    22741 ggctaaaatt cgcgattacc agacagctaa gtcagctatt gaaacaggtg cttccttggc
    22801 cggtcagaat ctagcctact ctctttacca gtcctacaag tctcagggcg aggaaaatcc
    22861 ccaaactaag gttcaggcag ttgcacaggt tgaagcacag atttctcagt tagaatctag
    22921 tcttgctact taccgtgtcc agtatgcagg ttcaggtacc cagcaagcct atgcgtcagg
    22981 gttaagcagt caattggaat cccttaaatc ccaacacttg gcaaaggttg gtcaggaatt
    23041 gacccttcta gcccagaaaa ttttggaggc agagtcaggt aagaaggtac agggaaatct
    23101 tttagacaag gggaaagtta cggcgagtga ggatggggtg cttcatctta atcctgagac
    23161 cagtgattct agcatggttg cagaaggtgc cctactagcc caactttatc catctttgga
    23221 aagagaaggg aaagccaaac tcacagctta tctaagttca aaatatgtag caagaatcaa
    23281 ggtcggtgat tctgttcgct atactacgac tcatgatgcc gggaatcaac ttttcctaga
    23341 ttctactatt acaagtattg atgcgacagc tactaagact gagaaaggga atttctttaa
    23401 aatcgaggcg gagactaatc taacttcgga gcaggctgaa aaacttaggt acggggtgga
    23461 aggccgcttg cagatgatta cgggcaagaa aagttaccta cgttattatt tggatcaatt
    23521 tttgaacaaa gagtaatgtt cgtgttttta gagttaaata atttttaaac tgtgagaaag
    23581 attcttcttg cagttttttc tttacaattt ttgaaaaaca tctactattt attcggttaa
    23641 attcttgtgt tttttggttt tttgtggtaa aatgtgctca agtaatacga aaggcgaact
    23701 ttaaaatgtc aaaacaattg atctattcgg gaaaagctaa agatatctat acaactgagg
    23761 atgaaaatct tattatttca acttacaagg accaggcgac tgctttcaac ggtgtcaaga
    23821 aggagcagat tgcaggtaag ggagtcttga ataatcagat ctcatctttt atttttgaga
    23881 aattaaatgt ggctggtgtg gcgactcact ttgtggagaa actttcagac acggaacaac
    23941 tcaataaaaa ggttaagatt attcctttgg aagtcgtgct ccgcaactat actgctggtt
    24001 ccttttcaaa acgttttggt gtggatgagg gaatcgcctt ggagactccg attgtcgaat
    24061 tttactacaa aaatgatgat ttggatgatc catttatcaa tgatgagcat gtgaaattcc
    24121 tacagattgc gggtgaccag cagattgcct acttgaagga agaaacgcgt cgtatcaatg
    24181 aactattgaa agtctggttt gctgagattg ggcttaaatt gattgacttt aagctagagt
    24241 tcggttttga caaggatggc aagattatct tggcagacga attttcacca gataactgcc
    24301 gcttgtggga cgctgatggc aaccacatgg ataaggatgt tttccgtaga ggattgggag
    24361 aactaaccga cgtttatgag attgtttggg aaaagttgca ggaattgaaa taatctgttt
    24421 gcaacggaaa accttcgtct ctcaactaaa aggactcagg ctgaaaaggt cccccagacc
    24481 ttttcactct gtagagaact aggtgaacta acagatgttt acgaaattgt ctgggaaaag
    24541 ttgcagggtt taaaataaca acctcaaggc tgtttgggaa tattgcaaga gctgaaataa
    24601 aggaataaga attgatggat aaacgtattt ttgttgaaaa aaaggctgat tttcaggtca
    24661 agtcagagag tttggttaga gagctccagc acaacttggg actgtcaagc ttgaaaagta
    24721 ttcgtattgt gcaagtatat gatgtatttg acttggctga ggacttgttt gcacctgcag
    24781 agaagcacat tttctctgag cagggaaccg accatgtttt agatgaagta tctgtgcagg
    24841 cggatcttgc taactatgct ttctttgcca ttgaaagtct gccagggcag tttgaccagc
    24901 gtgcagcttc gtcacaggaa gccttgcttt tgttgggaag ttcgagtgac gtgacagtca
    24961 acacagccca actttacttg gtgaataaag atattgatgc gactgagttg gaagctgtca
    25021 aaaactacct gctcaatcca gttgattctc gtttcaagga tatcacgaca gggattgcca
    25081 agcaggagtt ttcagagtca gacaagacca ttccaaaatt gactttcttt gaaagctata
    25141 cagcagaaga ctttgctcgc tacaaggccg agcaagggat ggccatggaa gtggatgatt
    25201 tgctctttat ccaagactac tttaagtcaa tcgggcgcgt gccaactgag actgaactca
    25261 aggttttgga cacttactgg tctgaccact gccgtcatac gacttttgag acagagttga
    25321 aacacatcga cttttcagct tctaaatttc aaaagcaatt gcagtcaacc tatgacaagt
    25381 atattgccat gcgcgaggag ttaggtcggt ctgaaaaacc acaaaccttg atggatatgg
    25441 cgactatttt cggtcgttat gagcgtgcta acggacgatt ggatgatatg gaagtctctg
    25501 acgaaatcaa tgcctgctca gttgaaattg aagtggatgt tgatggtgtc aaggaacctt
    25561 ggctcctcat gtttaaaaat gaaacccaca accatccaac agaaattgag ccatttggtg
    25621 gagcggctac ctgtatcggt ggagctattc gtgatccgtt gtcaggccgt tcctatgttt
    25681 accaagccat gcgtatttca ggtgctggtg atattacagc accgatttcg gaaactcgtg
    25741 ctgggaaatt gccacaacaa gtcatttcta aaacagcagc tcatggttat tcttcatatg
    25801 gtaaccagat tgggcttgcc acaacctacg ttcgtgaata cttccaccca ggctttgtag
    25861 ctaaacgtat ggaacttggt gccgttgttg gtgcggctcc caagggcaat gttgtccgtg
    25921 aaaaacctga agcaggtgat gtgatcatcc ttctcggagg caaaacaggt cgtgatggtg
    25981 tcggtggtgc gacgggctct tctaaggttc aaacagttga gtctgtagag actgctggtg
    26041 ctgaggttca aaaaggaaat gccatcgaag aacgcaagat tcagcgcctc ttccgtaatg
    26101 gcaatgtcac tcgtctgatc aagaagtcca atgactttgg ggcaggcggc gtctgtgtgg
    26161 ctatcggtga attggcagac ggtcttgaaa tcgacctcaa caaggtgcct cttaaatacc
    26221 agggcttgaa tggtacagaa attgccatct ctgaatcaca agaacggatg gcggtcgtgg
    26281 ttcgtcctga agatgtggat accttcgttg ccgaatgtaa caaagaaaat attgatgctg
    26341 ttgtggtggc gacagtaact gaaaaaccaa atcttgtcat gcactggaat ggtgagacaa
    26401 tcgttgactt ggagcgtcgt ttccttgaca ccaatggtgt gcgcgtggtt gtcgatgcca
    26461 aagttgtgga caaggatgtc aaactcccag aagagcgtca aacatctgct gaaacactgg
    26521 aatcagatac ccttacggtt ctatctgacc tcaaccatgc aagtcaaaaa ggattacaga
    26581 ctatctttga ctgctctgtt ggacgctcaa cggttaatca cccacttggt ggtcgttacc
    26641 aactcacacc aactgaggca tctgtgcaga aattgccagt tcaacacggt gtgactcata
    26701 ctgcgtcggt cattgctcaa ggtttcaacc catatgtagc tgaatggtct ccataccacg
    26761 gtgctgctta tgcggttatc gaagcaactg ctcgtttggt ggctgctggt gccaactggt
    26821 ctaaggctcg tttctcttac caagaatatt tcgagcggat ggacaagcaa gcagagcgtt
    26881 tcggtcagcc agtagctgct cttctaggtt ctattgaagc acaaattcag cttggcttgc
    26941 catctatcgg tggtaaggac tccatgtctg gtacctttga agaattgacc gttccgccaa
    27001 ccttggttgc ctttggggtg acgacggcag atagccgtaa ggtgctctct ccagaattta
    27061 aagctgttgg ggaaaatatc tactacatcc caggtcaagc cctctctgca gagattgatt
    27121 ttgacttgat taagaaaaat tttgctcagt ttgaagcaag ccaagctgac cataaagtga
    27181 cgtctgcatc agctgtcaaa tacggtggtg tagttgaaag tttggctctt gctacctttg
    27241 gaaactatat tggtgcagag gtgaccttgc ctgaacttaa aacagctttg acagctcaat
    27301 taggcggctt tgtcttcaca tctcctgaag aaattgctgg agtagagaag attggacaaa
    27361 cgaaagcaga ctttacactg actgtcaacg gtgtgaagct agatggacac aagcttgaca
    27421 gtgcatttca agggacattg gaagaagttt acccaacaga atttacccaa gcgaaagaac
    27481 tagaagaagt accagctgtg gcatcagatg ttgtgattaa agccaaagaa aaggttgaaa
    27541 aacctgtggt ttacatccca gtctttccag gaaccaactc agaatatgat tcagctaagg
    27601 ccttcgaaaa agaaggtgca gaggtcaatt tggtgccatt cgtgaccttg aatgaagaag
    27661 ctattgtcaa gtcagttgaa actatggttg acaatatcga caagactaat attctcttct
    27721 ttgccggtgg attctcggct gcggatgaac cagatggttc agctaagttt atcgtcaata
    27781 tcctgcttaa tgaaaaagtg cgtgtggcta ttgatagctt tatcgcccgt ggtggcttga
    27841 ttatcggtat ttgtaatgga ttccaagcct tagtcaaatc gggtctccta ccatacggaa
    27901 actttgaaga tgctaacagt actagtccaa ccctcttcta caatgatgcc aaccaacacg
    27961 tggccaagat ggtggaaact cgtattgcca ataccaactc accatggttg gctggtgtgc
    28021 aagtgggcga tatccacgct attcctgttt cgcacggtga agggaagttt gtcgtgacgg
    28081 ctgaggaatt tgcagagctc cgtgacaatg gacaaatttt cagccaatac gttgacttta
    28141 acggtaaacc aagtatggat tctaagtaca atccgaatgg ttctgtccat gccatcgaag
    28201 gaattaccag caagaatggt caaatcatcg gtaagatggg ccactcagaa cgttatgagg
    28261 atggtctttt ccaaaatatc ccaggcaata aagaccaaca cctgttcgca tcagcggtta
    28321 aacatttcac tggaaaataa gacttacaga ttttctaata gatagtatca gtaatgtaaa
    28381 agtcatgtaa atctagctct tgatgattac aaatgaaaat taggtataaa aaatgacata
    28441 cgaagtaaaa tctcttaatg aagaatgtgg tgttttcggt atttggggac atccagatgc
    28501 tgctaagttg acctattttg gactccacag tcttcaacac cgtggtcagg agggggcagg
    28561 aatcctctcc aatgatcaag gacaactgaa gcgccatcgt gacatggggc ttttatcaga
    28621 agttttcaga aatccagcca atttggataa attgacagga gctggtgcga ttgggcatgt
    28681 gcgttatgcg actgctggcg aagcttctgt tgataacatc caacccttcc tcttccgttt
    28741 tcacgatatg cagtttggtt tggctcataa tggaaatctg accaatgcag cctctctcaa
    28801 gaaagaactg gaacaaagag gagcaatttt cagcgcgact tcggactctg aaatcttggc
    28861 tcacctcatt cgtcgcagtc ataatcctag cctgatgggc aaaatcaagg aagcgctcag
    28921 ccttgtcaaa ggtggttttg cctatatctt gctgtttgag gacaagttga ttgcggctct
    28981 tgaccctaat ggctttcgtc cgctttctat cgggaaaatg gccaacggag cggtggttgt
    29041 ttcctctgaa acctgtgctt ttgaggtcat tggtgtcgag tggatttgtg atttgaagcc
    29101 aggtgagatt gtgatcattg atgacgaggg cattcagtat gacagctata cagatgatac
    29161 ccagttggcg atttgttcta tggagtatat ctactttgcc cgccctgatt ctaatatcca
    29221 cggtgtcaat gtccatacgg cacgtaagag aatgggagcg caattggcgc gagaatttaa
    29281 gcatgaggca gatattgtag ttggtgtgcc caattcttcc ctaagcgcgg ctatgggatt
    29341 tgcggaagaa tcaggcttac caaatgaaat gggtctgatc aaaaaccaat acacccagcg
    29401 aacttttatc caaccgactc aagaattgcg ggagcaagga gtgcggatga aactgtctgc
    29461 tgtttcgggt gttgtcaaag gcaaacgtgt ggtcatggtg gatgattcca ttgtacgtgg
    29521 aacaacctct cgtcgtatcg ttcagctctt gaaagaagcg ggtgcgactg aggttcacgt
    29581 tgccattgga agtcctgcac tagcgtatcc atgtttctac gggattgata tccagacccg
    29641 tcaggagctg attgcagcca atcatacggt cgaagaaact cgccaaatca ttggtgcgga
    29701 cagtctgact tatctttcaa ttgatggctt gattgagtcg attggtatcg aaacagatgc
    29761 gccgaacggt ggtctctgtg tcgcttactt tgacggtgac tacccaacgc ctctctatga
    29821 ctacgaagaa gactatcgta gaagtttgga agaaaagacc agtttttaca agtaggcgac
    29881 agattctcca ttaaagaaaa ggaaaaaaca aatgacaaat aaaaatgcat atgcctcacg
    29941 tctcactact gactaaaggc ttaagcattt agtcagtaga cgctttgtcc tataggatca
    30001 aagctagagc cctgactagt atttttagat aaaaagatgg tttatctaaa aatacgtcgc
    30061 agtctttctc aaaaaaagaa aaggaaaaat aaaatggcaa ataaaaatgc gtacgctcaa
    30121 tctggtgtgg atgttgaagc gggttatgaa gttgttgaac ggattaaaaa gcacgtggct
    30181 cgtacggagc gtgcaggtgt catgggagct cttggtggct ttggtggtat gtttgacctt
    30241 tccaagactg gggttaaaga acccgtcttg atttcaggga ctgacggtgt cggaaccaag
    30301 ctcatgttgg ctatcaagta cgacaagcac gataccatcg ggcaggactg tgtggccatg
    30361 tgtgtcaacg acatcattgc tgcaggtgcg gaacccctct attttctcga ctacgtagcg
    30421 acagggaaga atgaaccagc taagctagaa caagtggttg ctggtgtggc agaaggttgt
    30481 gtgcaggctg gtgctgccct catcgctggg gaaacggctg aaatgccggg catgtacggc
    30541 gaagacgact atgacttggc tggttttgcg gtcggtgtgg ctgaaaaatc tcaaatcatt
    30601 gacggttcaa aggtggtaga gggagatgtt cttctcggac ttgcttcaag tgggattcac
    30661 tcaaatggtt actctttggt tcgtcgtgtc tttgcggatt acacaggtga ggaagtccta
    30721 ccagaattgg aaggcaagaa acttaaggaa gttctacttg agccgactcg tatctatgtc
    30781 aaggctgtct tgccgctcat caaagaagag ttggtcaacg gcattgccca catcacaggt
    30841 ggtggcttta tcgaaaatgt ccctcgtatg tttgcagatg acctagctgc tgaaattgat
    30901 gaaagcaaag ttccagtgct tccaattttc aaagcccttg aaaaatatgg tcagattaaa
    30961 cacgaagaaa tgtttgaaat cttcaatatg ggtgtgggac ttatgttggc ggtcagccct
    31021 gaaaatgtag agcgtgtaaa agaattgttg gatgaagcag tctatgaaat tggtcgcatc
    31081 gtcaagaaag aaaacgaaag tgtcattatc aaatgaaaaa aatagcggtt tttgcctctg
    31141 gtaatggctc aaattttcag gtgattgccg aagaatttcc agtggagttt gtcttttcag
    31201 accatcgtga tgcctatgtg cttgagcgtg caaagcagct cggcgttctg tcctatgctt
    31261 ttgaactcaa ggagtttgag agcaagacag actacgaagc agcccttgtc gaactcttgg
    31321 aagaacacca gattgacttg gtttgcctag caggctacat gaaaatcgtt ggaccaacct
    31381 tattgtcggc ttatgaaggt cggattgtca acattcatcc agcctacttg ccagaatttc
    31441 caggagctca tgggattgag gatgcttgga atgctggcgt gggtcagtct ggtgtgacca
    31501 ttcactgggt ggattcgggt gtggatacag gccaggtcat caaacaggtt cgtgtgccac
    31561 gactagctga tgataccatt gacagatttg aagctcgcat ccatgaagca gagtacaggt
    31621 tgtatccgga agtagtgaag gctctattta cagattgact ttttgatgat tcatatgata
    31681 tctttgattt taaattggag tcagtgtttg ttgaagacgg cttcaaacgg aggtatttgt
    31741 aatgttagaa tctaaaaaaa caactcgata tgtattttat gtctatctga tgttattaac
    31801 ttggggaatc ttatttaagt ttgaaacaaa tcctgaattt atagcatttt tcttagctcc
    31861 aaggtatatc aattggattc cattttcaga accactaata gtcgatggaa aaattgtttt
    31921 tgctgaaatg ttatttaatc tgatttcctt tattccatta ggtgtttgtt tccctttgat
    31981 aaaaactaat ttatctagtt taagaatagt cgggacaggt ttcttgatta gtttattgtt
    32041 tgagtgctta cagtatattt tagcaatagg tataacagat ataacggatt tgactttaaa
    32101 tacgctaggt gtctgtgtag gcttactgat ttatcaaatt tttataagag tgttcaaatc
    32161 acagactaga aaatggatca atatcttagg tatgcttagc cttggttttg cttatcttgt
    32221 tttactgtta ctgcatttaa ttggtgttta actaatgatt aaaaaggaga atataatgac
    32281 taaacgcgtc ttaatcagcg tctcagacaa agcgggcatt gttgaatttg cccaagaact
    32341 aaaaaaactt ggttgggaga ttatctcgac aggtggaact aaggttgccc ttgataatgc
    32401 tggggtggag accattgcga tcgatgatgt gacgggcttc ccagagatga tggatggccg
    32461 tgttaagact cttcatccaa atatccacgg gggtcttctc gctcgtcgtg atttggatag
    32521 ccacttggaa gcagccaatg agaaccaaat tgagcttatt gaccttgtag tggtcaacct
    32581 ttacccattt aaggaaacta tccttaaacc agatgtgact tatgctgatg cagttgaaaa
    32641 tatcgatatt ggtgggccgt ctatgcttcg ttcagcagcg aaaaatcatg ccagtgttac
    32701 agttgtggta gatcctgctg actacgctgt ggttttggac gaattggcag caaacggcga
    32761 aacctcttat gaaactcgcc aacgtttagc agccaaagta tttcgtcaca cagcggctta
    32821 tgacgccttg attgcagaat acttcacagc tcaagtgggt gaaagcaagc ctgaaaaact
    32881 cactttgact tatgacctca agcaaccaat gcgttacggt gagaatcctc aacaagacgc
    32941 ggacttttac cagaaagctt tgcctacaga ttactctatt gcttcagcca aacagctcaa
    33001 cgggaaagaa ttgtcattta ataatatccg tgatgcagat gctgctatcc gtatcatccg
    33061 tgacttcaaa gatagtccaa ccgttgtggc tctcaagcat atgaatccat gtggtatcgg
    33121 ccaggcggat gacatcgaga cggcttggga ttatgcttat gagtctgacc cagtgtctat
    33181 ctttggtggt atcgttgttc ttaaccgtga ggtggatgct gcgacagctg agaagatgca
    33241 cggcgttttc ctcgaaatca tcattgcacc aagctatacg gatgaagcgc tagctatttt
    33301 gatcaataaa aagaaaaact tgcgtatcct tgccttgcca tttaatgctc aagaggctag
    33361 cgaagtggaa gcagaataca caggtgtagt cggtggactt ctcgtgcaaa atcaagacgt
    33421 ggtcaaggaa agcccagctg actggcaagt ggtgaccaaa cgtcagccaa ccgagacaga
    33481 agcgactgct cttgagttcg cttggaaggc tatcaagtac gtcaaatcaa atggtattat
    33541 cgtgaccaac gatcacatga cacttggtgt tggtccaggt caaaccaacc gtgtggcttc
    33601 tgttcgcctt gccattgacc aagccaaaga tcgtctggac ggggcggtcc ttgcttcaga
    33661 tgccttcttc ccatttgcgg ataacgtgga agaaatcgcc aaagcaggaa ttaaggccat
    33721 catccagccc ggtggctctg tccgtgacca agaatccatc gaagcagcgg ataaatatgg
    33781 cttgactatg gtctttacag gtgtgagaca ttttagacat taagaagata aaagggaaga
    33841 aaacagtttc tttccttttt tggcttaaaa tgctaactga aacaagatta aaacgaactt
    33901 ttttgatata atgttggtaa ataattcgca aaagaggttg aggaatgaaa ctgcttgttg
    33961 tcggttctgg tggtcgtgag catgcgattg ctaaaaagtt acttgaatca aaagacgtgg
    34021 aaaaagtctt tgtagctcct gggaatgatg ggatgactct ggatggtttg gaattggtaa
    34081 atatctctat ttccgaacat tctaaattga ttgacttcgc aaagaccaat gatgttgctt
    34141 ggacctttat cggtccagat gatgcccttg ctgctggtat cgtggatgat tttaaccaag
    34201 ctggacttaa ggcctttggt ccgactaggg ctgcagcgga gctggagtgg tccaaggatt
    34261 tcgccaagga aatcatggtc aaatacggcg ttccgacagc aacatatggc acattttcag
    34321 atttcgagga agccaaggcc tatatcgaaa agcatggtgc gcctatcgta gtcaaggcgg
    34381 atggcttggc acttgggaag ggtgtcgtcg ttgctgagac ggttgagcaa gcggtcgaag
    34441 ccgctcatga gatgcttttg gacaataaat ttggtgactc aggtgcgcgc gtggttattg
    34501 aggaattcct tgaaggagag gaattttcac tctttgcctt tgtcaatggt gataagttct
    34561 acatcatgcc aacggctcag gaccacaaac gtgcctatga tggcgacaaa gggcctaaca
    34621 cgggtggtat gggtgcctat gcgccagtcc cacacttacc acagagtgta gttgatacag
    34681 cggttgacac cattgtcaag ccagttctag aaggggtgat taaagaaggt cgcccttatc
    34741 tgggagttct ttacgcaggg cttatcctga cagctgatgg accgaaagtc attgagttca
    34801 acgctcggtt cggagatcca gaaactcaga ttatcttgcc tcgcttgacc tctgactttg
    34861 ctcaaaatat cacagatatc ctggatagca aggagccaaa tatcatgtgg acggacaagg
    34921 gtgtgactct gggtgtggtt gtcgcatcca agggctaccc gctagactat gaaaggggcg
    34981 ttgagttgcc agcaaagaca gaaggcgatg tcatcaccta ctatgcaggg gctaagtttg
    35041 cggaaaatag cagagcactg ctctcaaacg gcggacgagt ttatatgctc gttaccacag
    35101 cagataccgt caaagaagcc caagccagca tctaccaaga actatcccaa caaaaaatag
    35161 aaggactctt ctaccgaaca gatatcggaa gcaaggcaat taagtaaaga tataagaata
    35221 acgcgccgta gtcgccaaac acgataatgg tcgtcgtggt gaaaagacca gaacagtgaa
    35281 tgttctggtc agggggaaac ttggagacct taggctcaaa gtttaggaat gaaaccgaag
    35341 gtttgcttcc gcctccatca cctaagacca ttatcaaaaa gaaaaataaa aattcacaaa
    35401 atacgttaat gatcgtatgg tttgcgagcg ttagcgagct aatatagaac aatcaccgcc
    35461 gttgtgaaag aacgattgga tgataatcca atcgttcagg gaaattggaa gaccttgggt
    35521 ttccaattta ggcatgagac acctttggtg gctgctgccg tccctcacaa gctaaggtga
    35581 ttgttgaaaa agaggaaaaa ggagaagaaa tgaaaccagt aatttccatc atcatgggct
    35641 caaaatccga ctgggcaacc atgcaaaaaa cagcagaagt cctagaccgc ttcggtgtag
    35701 cctacgaaaa gaaagttgtt tccgcacacc gtacaccaga cctcatgttc aaacatgcag
    35761 aagaagcccg tagtcgtggc atcaagatca tcatcgcagg tgctggtggc gcagcgcatt
    35821 tgccaggcat ggtagctgcc aaaacaaccc ttccagtcat tggtgtgcca gtcaagtctc
    35881 gtgctcttag tggagtggat tcactctatt ctatcgttca gatgccgggt ggggtgcctg
    35941 ttgcgaccat ggctatcggt gaagctggag cgactaacgc agctctcttt gccctccgtc
    36001 tcctctctgt agaagataag tccattgcgg atgcacttgc caactttgct gaagaacaag
    36061 gaaaaatcgc agaggagtcg tcaaatgagc tcatctaaaa caatcggaat tatcggtggc
    36121 ggtcaactgg gtcagatgat ggccatttct gctatctaca tgggccacaa ggttatcgcg
    36181 ctggatcctg cggcggattg cccggcctct cgcgtggcgg aaatcattgt ggcaccttat
    36241 aacgatgtgg acgccctccg tcagttggca gaccgttgcg atgtcctcac ttacgagttt
    36301 gaaaatgtcg acgctgacgg tttggatgcc gttatcaagg atggacaact ccctcaagga
    36361 acagatctgc tccgcatttc gcaaaatcgt atttttgaaa aggacttttt gtcaaacaag
    36421 gctcaagtca ctgtggcacc ctacaaggtc gtgacttcta gcctagactt ggcagatatc
    36481 gacttgtcga aaaactatgt cctcaagact gcgactggtg gctacgatgg tcatggacaa
    36541 aaggttattc gttcagaagc agacttggaa gcagcctatg cgctagcaga ctcagcagac
    36601 tgcgtcttgg aagaatttgt caactttgac cttgagattt ctgtcatcgt gtcaggaaat
    36661 ggcaaggagg tgacgttttt cccagttcag gaaaatatcc accgcaacaa tatcctgtct
    36721 aagaccatcg taccagcccg catttctgaa agtctagcag acaaggctaa agctatggca
    36781 gtgcgaatcg cagaacaact caacttgtct ggaactctct gtgtggaaat gtttgcgact
    36841 gatgatgacg ttattgtcaa tgaaatcgcc ccacgaccac ataactctgg gcactattct
    36901 attgaagcct gtgatttctc tcagtttgac acccatattc tcggtgttct cggagcacca
    36961 ttaccagtca tcaaactcca tgcgccagcc gttatgctta atgtcctcgg tcagcatgtc
    37021 gaggctgctg agcaatatgt gacagaaaat tcaagcgccc acctccacat gtatggtaaa
    37081 atagaagcga agcacaaccg caagatggga catgtgacct tgtttagtga tgtaccggat
    37141 agtgtggatg agcttgggga agggattgat ttttaggaca agtctatgat acaaattatc
    37201 gttaatacat ttattgaaaa ggataagact ggagcagttg ttgaagtgtt gtatgccagt
    37261 gctgaccaag ataaggtaca agctaaatat gaagaactag ctgcacaata ccccgaaaat
    37321 tatttagcta tctatgatgt accgctggat acggatttga atacactaga tcattacccg
    37381 tctgtgttta ttgaaaaaga ggagtttgag tagaaatctt ggtttaccta gatagcttat
    37441 tcccaacagc ttaagaagaa aggaaaaatt aacacatgat caaccgttac tctcgccctg
    37501 agatggcgaa tatttggagt gaagaaaata aataccgtgc ttggcttgag gtggaaatcc
    37561 tctctgacga ggcatgggct gagttggggg aaatccctaa ggaagatgtg gctttgattc
    37621 gcaagaaggc ggactttgac atcgaccgta ttttggaaat tgagcaggag acgcgccacg
    37681 atgtggtggc tttcacgcgt gcggtttctg agactcttgg tgaagagcgc aagtgggttc
    37741 actatgggtt aacttctacc gacgtggtgg atactgctta tggttacctc tacaagcagg
    37801 ccaacgacat catccgtcgt gaccttgaaa acttcactaa tatcatcgct gacaaggcca
    37861 aggagcacaa gttcaccatc atgatggggc gtactcatgg tgtgcacgct gagccgacaa
    37921 cctttggtct taaattggca acttggtaca gcgaaatgaa acgcaatatc gagcgtttcg
    37981 agcatgcagc tgctggtgta gaagctggta agatttctgg tgcggttggg aactttgcca
    38041 atatcccacc atttgtagag gagtatgtct gcgataaact tggcatccgt gcccaagaaa
    38101 tctctacaca agtccttcct cgtgaccttc acgctgagta ctttgcggtt cttgccagca
    38161 ttgcgacttc aatcgaacgt atggcgactg agattcgtgg tctacaaaaa tctgagcaac
    38221 gcgaagtaga agagttcttt gctaaagggc aaaaagggtc ttcagcaatg cctcacaaac
    38281 gcaacccaat cggttctgaa aatatgactg gtctggcgcg tgtcattcgt ggtcacatga
    38341 ttacggctta tgaaaacgtc gctctctggc acgaacgcga tatttctcac tcatcagctg
    38401 agcgtatcat cacaccagat acgaccattt tgattgacta catgctcaac cgttttggaa
    38461 atatcgtcaa gaacttgaca gtcttcccag aaaatatgat ccgaaacatg aactcgactt
    38521 ttggtcttat ctttagccaa cgggctatgt tgacattgat tgaaaaaggc atgacccgtg
    38581 agcaagccta tgacttggtg caaccaaaaa cagcctactc ttgggacaac caagtagact
    38641 ttaaaccact tcttgaggca gattcagaag taacatcacg tctcactcaa gaagaaatcg
    38701 atgaaatctt caacccagtt tattacacca aacgagtgga tgatatcttt gaacgtcttg
    38761 gactaggtga ttaattaaaa aataaacagc gagcttcaat ctcgctgttt attttttatc
    38821 gaaaagactt agtcttcttt tcttttagtg agtccatagg ctgctagtgt ggacatgagt
    38881 cctgcgacta ctagtcctgc agaatcgtga gttcctgttt caggaagttt tttctctgtt
    38941 accacaggag ctggatcttg aggaagagct ttgctttcct cagcaggagc agttgatgga
    39001 gctggttggc ttgggatttc tagttttggt ttttcttcag caatagcggc ttgtccgttt
    39061 tcatcgccta catgtgttac catagttccg acttcgacta tttgagtaac ggcttcctgt
    39121 gctacgacac tatttacaag tgttttcact tccttaccat cggcagaagt gctcacagag
    39181 tagaagttgc tacgatgtcc attgacgccc ttagtaatga cttgtgtttt tcctttgagt
    39241 aagagtggat tttcacgagt cactgtggta aatggaattt cttctttttg gatatccagt
    39301 ctaggtttta cctcagtagt tggtgcaaga ccactttcat cacccttgtg agttacagga
    39361 gcgccaactt caaccacttg gtttataact tctttggtta cctggctatc aaggactgtt
    39421 tctgttgttt ttccattttc agtgagtaca gagatgtaat gagttcgttc acctttgact
    39481 cctgctgtga taatattttc ctgaccagct gggaggttag gattttcttt cttgataact
    39541 tcaaatggaa tttcttcagt tcttgtgatg agttctggtc tggtttcaac attggcagcc
    39601 acttcatttt catctaggct tcctgaatga gttgcagctg gtttgaggcc ttgaagagcg
    39661 gcttttaggt tggctacaag tgtgtcaagc tcagcttgtt tattacggtt gaggttgtaa
    39721 tttagagctg ttttagctgc gtcaagggtc tcaagacttt ctttactata tccttctaag
    39781 tttgtaggaa ttttagctaa ttcttcgcgg agagcattat aattagcacg gaagtagtct
    39841 ttgttgtggt ctgcaaaggc agtcatgagt tcaaagattt cctcttcctt gtattcagcg
    39901 cttggtctat ctgcccagat tgaaagcata cttccgactg ttggaagatc tacttcagga
    39961 tatttggtag aagctagttg attgaatggt gtttttccag tattctcaat agctttcttg
    40021 aggaaaccac caccatcttc tggtttttga ccaagaatgt agtaccagtc accgttggta
    40081 ttcaagaatt tatagccttt gcttgctagg tattgaggtg atgcgaggtt atatccccac
    40141 cagcctttag accagtaaga aatcaagaca tctttgtcaa actgaacatc gtccttgtct
    40201 tcatagtaga agccatcgtt gaaggccatt ggttgaagcc ctctttcttt ggccatagct
    40261 gcgagggtgt tggcatattc ggcaaatttg ccatagagtt gataccactt gaggtagtac
    40321 cagccttggg cactagtcgc atcgttggcg tattcgtcag taccaaagtt gaaaatcttt
    40381 gttttacctg caaagaagtc catgtattta ccgatgaggg cttttacaaa gttcatcgct
    40441 tcttcgtttt tcaagtccat agttgttttt gaaactttat caaagtgggc ttgaggattt
    40501 ttaataccta atttttccat ggcaaccagc atagcatcca tgtgacctgg actgttaata
    40561 gctgggatga gaccgatgtc cttagattta gcgtattcaa ttagctctgt tacttctgcc
    40621 tgtgttagtg cagtaccgtt tggatcgtcg tagtaagctt tagttccttc gataatagct
    40681 tttttaacgt catcactagc ataggttttt ccgttggcag taatggtcat atcatcgagt
    40741 agaaagcgaa gtccgtcatt tcctagaagg agatggacat cagaatatcc gagctcactg
    40801 gccttgtcta cgatgcgttt gagctggttc agagtaaagt atttgcgtcc agcatcgatt
    40861 gagattacct tgtttttggc aagtttttca acctcacgtt tagcttcttc ttctttttga
    40921 gcttcaggcg tgagggtcaa gttgttgaca gtttcttgaa gtttagcaat ggcttgatca
    40981 atcgtatctt gttgggcacg gctaaggttg ctatcgagag agcgaatagc tttttcagct
    41041 tcttttacgg ccgtgacgct ttctgcagta taacggttca ggtcttttgg tacctcgtta
    41101 agtgcttgct ctgcagattc ataatcagct gcgaagtatt cagcgttggc atttgcaaaa
    41161 tgacgcatga gtttgaagag gcgtgatggt gaataacgtg cagatggagt gtcagcccaa
    41221 gcagctacca taccaccgat gattgggata tcagctcctt ctgtttttgg tacagaagtg
    41281 attggtgtgt ttttaatacc attgagcccc tgatcgagat tgtaccagcc ttggccatca
    41341 gcgtttcgtc caagaacgta gtaccaagca tcattggtat taaggatttg gtgacctttt
    41401 tcagctagta gtttagaaga agcgacatcg tagcctcccc aaccaccagt ccacatagaa
    41461 acgatgatgt ctttgtcaaa actaccaaag cttgtgtcgc tattgtagta gataccgtcg
    41521 ttaaaggcca ttggtttgag accgtgcgat tttacaatac gagcgaggtc attggcgtag
    41581 gcaataaatt tttcatagcc ttttacaggg tagccttcgt ttggatagta tttatcagct
    41641 tgaagcacac tccaaccttt agcatctgtc gcatcattgg catattcatc aagtccgatg
    41701 ttgaagattt cagtcttttt cgcgaaataa gcagcatact tgtcgataag ggcttttgta
    41761 aaagcgacag cttgttcgtt gtcaagatcg acagtacggg ctgatttctt cccaaaatag
    41821 ctaaagttag ggttttggat tcccaattct ttcatggcat tgagaatcgc atccatgtgt
    41881 ccaggactat ttactgtcgg aatgagaccg atacctttat ctttggcata gttaatcaga
    41941 tctgtcattt gactttctgt taagtgattg ccgtttggat cgttgtaata atcatttgta
    42001 cctttttcaa tggcgcgttt gacatcgtca ctggcatagg tcttgccgtt agctgtgatg
    42061 ctcatatcgt ccaacatgaa acggagtcca tcatttccga ctaataggtg taaatcagtg
    42121 tagccataat gtttcgcttt atcgatgatt tccttgagct gttctggtga gaaatattta
    42181 cgtccagcat caatagaaac aattttcttt ttcgctagtt tttcatttac agttgcagca
    42241 cgttcctttc ctgcctctgt ttccggtttg tcagcctctg ctttcgcttc atctttttta
    42301 gctggtttat ccttgtcagt cttgtctgta tttgactctt tagaatcaac ctctttcgct
    42361 tcttcctttt tagggctagc ttcttctgcc tttttattag cagtttcttt ttcagcagaa
    42421 gttggagtta ccacttctgc tttatcacta ggagttgaac taacttcctc ttgtggtttt
    42481 tcttctgttt ttggaagact agctacctta tcagtagctg gagtttctgt ttctacagtt
    42541 tttggagctt ctggttgaag ctctgcttta ggtgtttcct cagtccgatt ttcggatgat
    42601 tgaggggaat cagaaaccgt atggatggtc ggttggtttt ctgtagtagt aggagtaact
    42661 ccatcggctg caacagtctg tgcttggaag gcaaatccaa ttagaacaga agctgctcct
    42721 acagcgtatt tacgaataga aaaacgctgt tgtttttcat gtttcattgc aaaacctcct
    42781 gattgcattg ttatattgat agcgattata taaatcaacg cctttatttt atttcttata
    42841 ttaacgagag tcaagaggag atgacaaaaa actataataa gtataaaaaa atataaaatt
    42901 taaacttaag atttcagatt ggtcggaaaa aatgaatata tatatctagt ataatttttg
    42961 gttctatttc tataaaatat tccacaaatt atagaatctt ccaaaaatag gtaagcgcta
    43021 cctttttggt gtggtataat aagcatagaa aaagcccaag cgattagctc aggctttctt
    43081 cttagtgatc acggtcacat gagataaatt taatcttgta gtaatcagat cgtttgtaag
    43141 tttcactgta ttctaaaact tggccagttg attcgagttt ggtgatttta gtttgtagga
    43201 cagtagggaa ttgttcatcg actccgagga ctgaagccgc atgttctgga gttggaaaga
    43261 ctatttcgtt gatttcttca aagtgttcat cattcatgtg aatgtggtag tctaacttga
    43321 aacgattata gatagaacta tagtattcaa ggtttggata atttgcgttg atatattgtt
    43381 ctgggatgta ggatgtatgg tagatataaa cgacaccgtt tgattcgcgg atacgttcaa
    43441 tcttgtagta gaattgatcg ccgcgtagac ccaatttttc caagtaaaca agcttgtttc
    43501 cgcgttcaat tgaaagaaca gttaccttat catctttagc attgaagagt tcaatatctg
    43561 aaaactctac aagcttgtgt ttgcgcgcac gtgaaacgaa ggttcctttt ccttgttggc
    43621 ggacaatata gccatctttg gcaaggtcgt ttaaggcgcg aacaactgtg atagagctga
    43681 catcgtacat tgaaatgagt tctgcttcag tgtaaaattt atctccactg ctaaactgcc
    43741 cagagatgat tttatttttt aattcgtctt ttatgtattg atatttggga attgccatat
    43801 ttttacctct attttccttc tccatagaaa attttaccat aaaagcgaaa aaaatagtag
    43861 tcttttgaat aaaaaattag aataatagtt tgaaaaatta agcgatatag ggattgctca
    43921 tattttataa atgtattcta tacaaaatta ttttaggtcc tatattccga atttaaagag
    43981 cgtttgaaat gggtctaaaa atgtttgaaa ctaattgaat gtgtgtataa tagtaaaaat
    44041 gcagtaaata tcccgacaat ttctattgac aattcaaaca gattggttta taattaatat
    44101 aacaacaaat gaaagcgcaa actttcgcgg tcggaaggta gttttatgac acgatttgag
    44161 atacgagatg atttctatct cgatggaaaa tcatttaaga ttttatctgg tgccattcat
    44221 tattttaggg ttcctccaga ggattggtat cattcgctct ataacttgaa ggctcttggt
    44281 tttaatacgg tagagactta tgttgcttgg aatttacacg agcctcgtga aggtgagttt
    44341 cattttgacg gtgatctgga tttagagaaa tttctccaaa tagcgcagga tttgggtctc
    44401 tacgcaattg tgcgtccgtc tccatttatc tgtgcggaat gggaattcgg tggcttacca
    44461 gcttggctct tgaccaagaa catgcgaatt cgctcatccg acccagcata tatcgaggca
    44521 gttggtcgct actatgatca gttattgcca agactggttc ctcgtttgtt ggacaatggt
    44581 ggcaatattc tcatgatgca ggttgaaaat gagtatggtt cttacggaga agataaggct
    44641 tacctgagag cgattcgaca gctaatggaa gagtgtggcg taacctgtcc cctctttaca
    44701 tcagatggtc catggcgagc tactctgaaa gctggaacct taattgaaga ggacctcttt
    44761 gtaacaggaa actttggttc taaggcacct tacaactttt cgcagatgca ggaattcttt
    44821 gatgaacatg gtaagaaatg gccactcatg tgtatggagt tctgggatgg ttggtttaat
    44881 cgttggaaag aaccgattat cacacgggat cctaaggaat tggcagatgc agttcgagaa
    44941 gttttggaac aaggctccat caatctttac atgttccacg gtggtacaaa ctttggtttc
    45001 atgaatggtt gctcggctcg aggaactttg gacctgccac aagttacgtc ttatgattac
    45061 gatgcccttc tggatgaaga aggaaatcca actgctaaat atcttgcagt caagaagatg
    45121 atggcaacac atttttcaga gtatccgcag ttggaaccac tctacaaaga gagtatggag
    45181 ttggatgcta ttccactagt tgaaaaagtt tctttgtttg aaaccttaga tagcttgtca
    45241 agtcctgtag aaagtctcta tcctcaaaag atggaggagc tgggacaaag ttatggctac
    45301 ctactttatc gaacagaaac aaactgggat gcagaagaag aaagacttcg tatcattgat
    45361 ggtcgagata gggcccagct gtatgtcgat ggtcagtggg ttaaaactca atatcagaca
    45421 gagattgggg aagatatttt ttatcaaggt aaaaagaaag ggctatctag gttagatatc
    45481 ttgatagaaa atatggggcg tgtcaactat gggcataagt tcttagcgga tacgcaacgt
    45541 aagggaattc ggacaggggt ctgtaaggat ctgcatttct tactaaactg gaaacactat
    45601 ccactcccac tagacaatcc tgagaaaatt gatttttcaa aaggatggac tcaaggacaa
    45661 ccagcctttt acgcttatga ctttacagtc gaagagccaa aagatactta cctagacttg
    45721 tctgagtttg gtaagggggt tgcctttgtc aatgggcaga atctaggacg tttttggaac
    45781 gttggcccaa ctctctcact ttatatccct catagctatc tcaaggaagg tgccaaccgc
    45841 atcattatct ttgaaacaga aggtcaatat aaagaagaga ttcatttaac tcgtaaacct
    45901 acactaaaac atataaaggg ggaaaactta tgacaattgt aggatgccgt attgatggac
    45961 gtttgatcca cggacaagta gccaatcttt gggctggaaa actaaatgtt tcacgcatta
    46021 tggttgtaga cgacgaagtt gtcaacaacg atattgaaaa gagtggtttg aaacttgcga
    46081 caccaccagg tgtgaaattg agtattttgc cagttgagaa agctgcagcc aatattcttg
    46141 ctggcaaata cgatagccaa cgtctcttta tcgtggctcg taaaccagac cgcttccttg
    46201 gtttggtaga agcaggtgta ccacttgaaa cccttaatgt tgggaatatg tctcaaacac
    46261 cagaaactcg ttctattaca cgttctatca acgtagtaga caaggatgtg gaagacttcc
    46321 acaaactggc agaaaaaggt gttaaactta ctgctcagat ggttccaaat gatccaattt
    46381 cagacttttt gagcttatta aaataggaaa aaatttttta ggaggtcatt gttatgatac
    46441 aatggtggca aattttactt ctcactttgt actcagctta tcaaatctgt gatgagttga
    46501 cgatcgtttc atctgcaggt tcccctgtat ttgctggttt cattactggt ttaatcatgg
    46561 gagatgtgac tactggttta cttatcggtg gtaacttgca actgttcgtt cttggggttg
    46621 gtaccttcgg tggtgcttct cgtatcgacg caacttctgg tgcggttctt gcgacagcct
    46681 tctctgtttc acaaggaatt gatgcaccgc ttgcaattac tacaatcgct gtaccagtag
    46741 cagctctctt gacttacttc gacgttcttg gtcgtatgac tactaccttc ttcgctcacc
    46801 gtgtggatgc tgcaatcgaa cgctttgact ataaaggtat tgaacgcaac tacttgcttg
    46861 gtgcgattcc gtgggctcta tctcgtgccc ttccagtctt ctttgccctt gcttttggtg
    46921 gtgcctttgt acaatcagta gtagacttcg ttgaagccta caaatgggtt gcagatggct
    46981 tgacactcgc aggacgtatg cttccaggtc ttggatttgc aatcttgctt cgttaccttc
    47041 cagttaaacg taaccttcac taccttgcta tgggatttgg tttgacagct atgttgactg
    47101 ttctttactc atatgtaaca ggtcttggtg gcgctgttgc tggtatcgta ggtactcttc
    47161 ctgctgaagt tgctgaaaaa attggtttcg tgaacaactt caaaggtttg tctatgattg
    47221 gtatttctat cgtaggtatt ttccttgcag tgcttcactt caaaaatagc caaaaagtag
    47281 ctgtagcagc accttctaca ccatcagaaa gtggggaaat cgaagatgac gaattctaat
    47341 tacaaactta caaaagaaga ttttaatcaa atcaacaaac gtagcttgtt tactttccaa
    47401 ttaggttgga actacgaacg tatgcaagct tctggttacc tttacatgat cttgcctcag
    47461 ttgcgtaaaa tgtatggtga tggaactcct gaattgaaag aaatgatgaa agttcatact
    47521 caattcttca atacttcacc attcttccat accattatcg ctggttttga ccttgccatg
    47581 gaagaaaaag atggtgtagg ttcaaaagac gctgttaacg gtatcaagac aggtttgatg
    47641 ggaccattcg ctcctcttgg ggatacaatc tttggttcac ttgtacctgc tatcatgggg
    47701 tcagtcgcag caactatggc tatcgctggc caaccttggg ggatcttcct ttggattgca
    47761 gttgcagtag cgtatgacat cttccgttgg aaacagttgg aatttgctta caaagaaggg
    47821 gttaacctta tcaacaacat gcaaagtacc ttgacagctt tgattgacgc tgcatctgta
    47881 cttggtgtct tcatgatggg tgctcttgta gcaacagtga ttaactttga aatttcttac
    47941 aagttgccaa tcggtgaaaa gatgattgat ttccaagaca tcttgaacca aatcttccca
    48001 cgtttgcttc cagcaatctt tactgccttt atcttctggt tgcttggtaa aaaaggtatg
    48061 aactctacta aagctatcgg tattattatc gtacttgctt tggctctttc tgcccttggt
    48121 cactttgcac ttggaatgta attccttatg actaaatcat taattttggt gagccatggt
    48181 cgcttctgtg aggagcttag aggtagcaca gaaatgatta tgggcccaca agacaacatt
    48241 tacacagtag ctcttcttcc agaagatggc ccagaagaat ttactgctaa atttgaagct
    48301 gttattgaag gattggatga tttcctagtc tttgcggatc ttctcggtgg gacaccttgt
    48361 aatgtggtga gtcgcttgat catggaaggt cgtgatattg acctttacgc agggatgaat
    48421 cttccaatgg tgattgaatt tatcaatgcg agccttacag gcgcagatgc ggactacaag
    48481 agccgtgctg cagaaagcat tgtgaaagtt aatgacctgt tagcgggctt cgatgatgac
    48541 gaagatgaat aatactcttc gaaaatctct tcaaactacg tcaacgtcgc cttgccgtag
    48601 gtatatgtta ctgacttcgt cagtcttatc cggcaacctc aaagcggtgc tttgagcagc
    48661 ctgcggctag tttcctacag attttagttg gaactcgatt caattcatgt gacaacgtga
    48721 aaatcgttag agcattttat atagaatata catgggaatg gagcttactc ccattcccat
    48781 atttaataga aaaagaggaa ctcaatgcta cattatacaa aagaagactt gctcgaattg
    48841 ggtgcagaaa tcactacgcg tgaaatctac caacagcctg atgtatggag agaagctttt
    48901 gaattttatc aagcaaaacg tgaagaaatt gcagcctttc tacaagaaat cgctgataaa
    48961 catgactata ttaaggttat cttgacaggt gctgggactt ctgcttatgt gggagatacc
    49021 ttgctacctt actttaagga agtctatgac gaacgcaaat ggaatttcaa tgctattgcg
    49081 acaacagata tcgttgccaa accagcaacc tatttgaaaa aagatgtggc aactgtcctt
    49141 gtgtcttttg ctcgtagtgg gaactcgcct gaaagtgtgg cgactgttga tttggccaaa
    49201 tccttggtgg atgagcttta tcaagtgacg attacttgtg caacagatgg taaattggct
    49261 cttcaagctc acggtgatga ccgtaatctc ttgctcttgc aaccagctgt ctctaatgat
    49321 gctggatttg ccatgacttc tagctttacg tctatgatgt tgacagctct cttggtcttt
    49381 gatcctacag aatttgctgt taagtctgaa cgttttgaag ttgtatctag tcttgcccgt
    49441 aaagttttag acaaggtaga agatgtcaaa gagctcgttg atttagactt taaccgtgtc
    49501 atctatctag gcgctggtcc tttctttgga cttgctcatg aagctcagct caagattttg
    49561 gaattaactg ctggtcaagt tgcgaccatg tatgaaagcc cagttggctt ccgtcacggt
    49621 ccaaaatctc ttatcaacga caatacagtt gttttggtct ttggtacaac gacagcctac
    49681 actcgtaagt acgacttgga cttggttcgt gaagttgctg gtgaccagat tgctcgtcgt
    49741 gttgtgcttt tgagtgatca agcttttggt cttgaaaatg tcaaagaagt ggtccttggt
    49801 tgtggcggtg tcttgaatga tatttaccgt gtcttccctt acatcgttta tgcccaactc
    49861 tttgctctat tgacttcact caaggtagaa aataaaccag atacaccgtc tcctacaggt
    49921 acagtaaacc gtgtagtaca aggtgtcata attcacgaat atcaaaagta agacagtgtt
    49981 tatgaattct tgacaagagg atttgtaaat tatcagataa accatagatt gtcagtacgc
    50041 tttctatggt ttgtttgctt gagagaaata gtaaaaggag aacagaatga aagcatacac
    50101 agagcgtgta tttggaaatg ttgagggcga ggatgtcttg gcctatcgat ttgagacaga
    50161 cggtggctac caacttgagg ttatgactta tggcgcgacc atcttgcgct atgtcgcacc
    50221 tgacaaggct ggaaattttg ccaatgttat cttgggattt gatgactttg atagttatgt
    50281 aggcaatagt cccaagcatg gagcaagtgt aggtcctgta gcgggtcgta ttgcaggtgc
    50341 gacctttgag ctcaatggta agacctatga ccttgaggtt aataatgcta gcaactgtaa
    50401 tcacagtggt tcaactggtt gggattccag cttgtttgaa gttgaagaag taagcgatca
    50461 tggcttgact ctctacacag agcgtacaga tgggacagga gggttccctg gaaatctcaa
    50521 gatttggatc agttatcact tggaagaaac tggtgcctat gaaatcatct acaaggtaac
    50581 gaccgatcag gatacgctgg tcaatccaac caaccacagc tatttcaact tgtctggtga
    50641 tttcacgcag acgattgacc gtcatgtctt ccaactaaac acagagggca tttactcaat
    50701 cgctcctgac ggtgttcctg ccaaaactcc agaagccaac cgtgatgtgg tcaaacacat
    50761 ctacaatggt accttgttga aggatatctt tgcagaagaa gatgagcaaa tccagctggc
    50821 atcaggtttg gatcacccat ttgcccttcc tgcaggccat gacaatgctg gattccttta
    50881 tgaccaaaat tcaggtcgct tcctgctttt caagacagaa gctccttgct ttgtggtcta
    50941 cacagcaaac tttgtggatg aaagtgtcat cataggaggt cagccaatgc tacagcacaa
    51001 tgggattgct cttgaagcgc aagctttacc agatgccatt cacagtgacc ttaaaggcca
    51061 agtcattctt aaagctggtc aaaccttcac cagtaagaca cgttatgaac ttgttgtgaa
    51121 ataaaaaaag ctggtcaaaa agtctttaaa attaaaataa cgcatattgt caggtgatga
    51181 aaagtttgat aatatgcgtt ttattatgaa atgatttact ttatcttatc ctgaaatggt
    51241 gttatttttg agttatctgg cttgactttc ttgatggaaa ttatataata gttgtaataa
    51301 caataattac aacgagaaaa gaggaccatt tatgacctat gaatacaaga gtcacattta
    51361 tttggcagag acagttttaa atgtaaagga tttggccagt caaacagcct tttatcagca
    51421 agttattggt ttagaaatct tatctcaaac tgagacagag tccattctag gccttggtgg
    51481 aaaagtcttg gtacagctga ttcaagcaca agagagcgga gaagtgaggg aacattatgg
    51541 tctttaccat ttggccattc tcttgcccac acgcaaggct ttggctgatg tcttgaaaca
    51601 cctgacagat ttgcaaattc ctcttgtcgg cggtgcagac catggttaca gtgaagctat
    51661 ttacttggaa gatttggagg gaaatggcat tgaactctat cgagataagc cagtttccac
    51721 atggaatatt cgagaagatg gacgcattat cggagtgacg gaggctcttg cggcgcagga
    51781 catttatgag ttgggggaaa gagtagagcc ctttatcctg gcagaaggta caagaatggg
    51841 gcatattcat ctttccgtca aggatagtcg aaagtccagc cagttttatc aaaaggtgtt
    51901 agggctagag aataaattca gtgtgcctgg cgctagttgg attgcggctg gagattacca
    51961 tcatcattta gcagttaacg aatggggagg aaaaggtctg gcttcgcgta agcaaggctt
    52021 accaggttta gcctactatg tcatcgaagt cgcacataaa aaagaactgt taacgattgc
    52081 ccaacgagca caagaagttg acgcaccaat caaatggatg acatcgagcc aattggaaat
    52141 cacagactca gatggcatcg tgacccgtat tcgtttagca aggtaaaatt aggaattttt
    52201 aataagttat aagtattgct agtttgcttt ttgaacggtc tttggtaaca gaactgcgtg
    52261 agctggatgg gtgagaattt tgacaaagaa gctgattaga gatataatga agaaaaatcg
    52321 agcaaggaaa aagaaatgac aaattcaaag tatattactt gtttgaaacg ttcagagggc
    52381 cagttgtgtg gaattcaaaa gatgattgaa ggagatcgtg actgtgctga tattgtgacg
    52441 caactgacag cagtgaaatc tagtgtagag cgcgtgattg agatgataat taccgaaaat
    52501 cttactgaat gtatcaatca gccactagat gattctgaag ctcaaaagga acgcctagaa
    52561 aaggctatcc gatacttgat taaacggaaa taaatgaatg ttttgagacg atatgtgcaa
    52621 aggtctatta actattagaa tagtttaatg tgagtcaaaa gaagttgtaa aaaatcttta
    52681 aaattaaaaa acgcatagta tcaggtattg aaatgtactg ccccccaaaa gttagattct
    52741 ttctgtctaa cttttggggg gcagtacata aaaaccttga tagtatgcgt tttattgtgg
    52801 ggagacttat actcttcgaa aatctcttca aaccacgtca gtttcacctt gccgtagata
    52861 tggttactga cttcgtcagt tc
//
LOCUS       DAVKMU010000008        51960 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKMU010000008 DAVKMU010000000
VERSION     DAVKMU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.008"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..839)
                     /gene="lytB"
                     /locus_tag="VW343_000542"
     CDS             complement(<1..839)
                     /gene="lytB"
                     /locus_tag="VW343_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU9755147.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQA"
     gene            complement(881..1867)
                     /locus_tag="VW343_000543"
     CDS             complement(881..1867)
                     /locus_tag="VW343_000543"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9755148.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1830..2630)
                     /locus_tag="VW343_000544"
     CDS             complement(1830..2630)
                     /locus_tag="VW343_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9755149.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            complement(2880..3260)
                     /locus_tag="VW343_000545"
     CDS             complement(2880..3260)
                     /locus_tag="VW343_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9755150.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3318..3677)
                     /gene="rplT"
                     /locus_tag="VW343_000546"
     CDS             complement(3318..3677)
                     /gene="rplT"
                     /locus_tag="VW343_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9755151.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3729..3929)
                     /gene="rpmI"
                     /locus_tag="VW343_000547"
     CDS             complement(3729..3929)
                     /gene="rpmI"
                     /locus_tag="VW343_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9755152.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(3962..4492)
                     /gene="infC"
                     /locus_tag="VW343_000548"
     CDS             complement(3962..4492)
                     /gene="infC"
                     /locus_tag="VW343_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9755153.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4508..4639)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4813..5988)
                     /locus_tag="VW343_000549"
     CDS             complement(4813..5988)
                     /locus_tag="VW343_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755154.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     gene            complement(5992..6579)
                     /locus_tag="VW343_000550"
     CDS             complement(5992..6579)
                     /locus_tag="VW343_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755155.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMTDYISLPIRKYSLGMKQRLVIAMYFLSQAKFWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            complement(6611..6799)
                     /locus_tag="VW343_000551"
     CDS             complement(6611..6799)
                     /locus_tag="VW343_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755156.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGSY"
     gene            complement(6979..9219)
                     /locus_tag="VW343_000552"
     CDS             complement(6979..9219)
                     /locus_tag="VW343_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9755157.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            complement(9203..9853)
                     /locus_tag="VW343_000553"
     CDS             complement(9203..9853)
                     /locus_tag="VW343_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU9755158.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            complement(9921..10490)
                     /locus_tag="VW343_000554"
     CDS             complement(9921..10490)
                     /locus_tag="VW343_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755159.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            complement(10712..11665)
                     /locus_tag="VW343_000555"
     CDS             complement(10712..11665)
                     /locus_tag="VW343_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU9755160.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEEGPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            11913..12338
                     /locus_tag="VW343_000556"
     CDS             11913..12338
                     /locus_tag="VW343_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9755161.1"
                     /translation="MELSIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDVLLLFTYLHMAAAPEL
                     ADAMLAAKITGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            12339..12752
                     /locus_tag="VW343_000557"
     CDS             12339..12752
                     /locus_tag="VW343_000557"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9755162.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGGGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            12698..13024
                     /locus_tag="VW343_000558"
     CDS             12698..13024
                     /locus_tag="VW343_000558"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755163.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(13391..13651)
                     /locus_tag="VW343_000559"
                     /pseudo
     CDS             complement(13391..13651)
                     /locus_tag="VW343_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(13654..14154)
                     /locus_tag="VW343_000560"
     CDS             complement(13654..14154)
                     /locus_tag="VW343_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755164.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            14190..14369
                     /locus_tag="VW343_000561"
     CDS             14190..14369
                     /locus_tag="VW343_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755165.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            complement(14347..15315)
                     /locus_tag="VW343_000562"
     CDS             complement(14347..15315)
                     /locus_tag="VW343_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9755166.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(15401..15616)
                     /locus_tag="VW343_000563"
     CDS             complement(15401..15616)
                     /locus_tag="VW343_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9755167.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(15625..16479)
                     /gene="cvfB"
                     /locus_tag="VW343_000564"
     CDS             complement(15625..16479)
                     /gene="cvfB"
                     /locus_tag="VW343_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9755168.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGCVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(16539..17096)
                     /gene="frr"
                     /locus_tag="VW343_000565"
     CDS             complement(16539..17096)
                     /gene="frr"
                     /locus_tag="VW343_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9755169.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(17105..17842)
                     /locus_tag="VW343_000566"
     CDS             complement(17105..17842)
                     /locus_tag="VW343_000566"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9755170.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(17926..19260)
                     /gene="trmFO"
                     /locus_tag="VW343_000567"
     CDS             complement(17926..19260)
                     /gene="trmFO"
                     /locus_tag="VW343_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9755171.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(19555..19809)
                     /locus_tag="VW343_000568"
     CDS             complement(19555..19809)
                     /locus_tag="VW343_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9755172.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            19804..20608
                     /locus_tag="VW343_000569"
     CDS             join(19804..20172,20174..20608)
                     /locus_tag="VW343_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9755173.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKKQLANHSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     CRDMKLFKMSRRNIGQAGKILSDSGYQGLMKIYPQVQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKIENIFAKVKTFKMFSTIYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(20597..21243)
                     /locus_tag="VW343_000570"
                     /pseudo
     CDS             complement(20597..21243)
                     /locus_tag="VW343_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(21352..21870)
                     /locus_tag="VW343_000571"
     CDS             complement(21352..21870)
                     /locus_tag="VW343_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9755174.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(22097..22966)
                     /gene="rsmI"
                     /locus_tag="VW343_000572"
     CDS             complement(22097..22966)
                     /gene="rsmI"
                     /locus_tag="VW343_000572"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9755175.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(22969..23286)
                     /gene="yabA"
                     /locus_tag="VW343_000573"
     CDS             complement(22969..23286)
                     /gene="yabA"
                     /locus_tag="VW343_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9755176.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(23326..24216)
                     /locus_tag="VW343_000574"
     CDS             complement(23326..24216)
                     /locus_tag="VW343_000574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9755177.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(24213..24851)
                     /locus_tag="VW343_000575"
     CDS             complement(24213..24851)
                     /locus_tag="VW343_000575"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9755178.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLELHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMDLAK"
     gene            complement(25071..25868)
                     /gene="proC"
                     /locus_tag="VW343_000576"
     CDS             complement(25071..25868)
                     /gene="proC"
                     /locus_tag="VW343_000576"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9755179.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFYQLLAKAGLLVELGES
                     LIDAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(25872..27134)
                     /locus_tag="VW343_000577"
     CDS             complement(25872..27134)
                     /locus_tag="VW343_000577"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9755180.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAIGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(27144..28274)
                     /locus_tag="VW343_000578"
     CDS             complement(27144..28274)
                     /locus_tag="VW343_000578"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9755181.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(28354..30237)
                     /gene="cbpE"
                     /locus_tag="VW343_000579"
                     /gene_synonym="pce"
     CDS             complement(28354..30237)
                     /gene="cbpE"
                     /locus_tag="VW343_000579"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9755182.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(30240..31127)
                     /locus_tag="VW343_000580"
     CDS             complement(30240..31127)
                     /locus_tag="VW343_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9755183.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(31117..31578)
                     /locus_tag="VW343_000581"
     CDS             complement(31117..31578)
                     /locus_tag="VW343_000581"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9755184.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(31575..32483)
                     /locus_tag="VW343_000582"
     CDS             complement(31575..32483)
                     /locus_tag="VW343_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9755185.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            32476..32673
                     /locus_tag="VW343_000583"
                     /pseudo
     CDS             32476..32673
                     /locus_tag="VW343_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(32725..33396)
                     /locus_tag="VW343_000584"
     CDS             complement(32725..33396)
                     /locus_tag="VW343_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9755186.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(33450..33671)
                     /locus_tag="VW343_000585"
     CDS             complement(33450..33671)
                     /locus_tag="VW343_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9755187.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            33943..34752
                     /locus_tag="VW343_000586"
     CDS             33943..34752
                     /locus_tag="VW343_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9755188.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(34933..35808)
                     /gene="aguB"
                     /locus_tag="VW343_000587"
     CDS             complement(34933..35808)
                     /gene="aguB"
                     /locus_tag="VW343_000587"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9755189.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(35818..36903)
                     /gene="aguA"
                     /locus_tag="VW343_000588"
     CDS             complement(35818..36903)
                     /gene="aguA"
                     /locus_tag="VW343_000588"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9755190.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(36900..38027)
                     /gene="nspC"
                     /locus_tag="VW343_000589"
     CDS             complement(36900..38027)
                     /gene="nspC"
                     /locus_tag="VW343_000589"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9755191.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEIMDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(38027..39286)
                     /locus_tag="VW343_000590"
     CDS             complement(38027..39286)
                     /locus_tag="VW343_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9755192.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(39283..40143)
                     /gene="speE"
                     /locus_tag="VW343_000591"
     CDS             complement(39283..40143)
                     /gene="speE"
                     /locus_tag="VW343_000591"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9755193.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            complement(40174..40311)
                     /locus_tag="VW343_000592"
     CDS             complement(40174..40311)
                     /locus_tag="VW343_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755194.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            40398..41113
                     /locus_tag="VW343_000593"
                     /pseudo
     CDS             40398..41113
                     /locus_tag="VW343_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(41079..42554)
                     /locus_tag="VW343_000594"
     CDS             complement(41079..42554)
                     /locus_tag="VW343_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9755195.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            43261..44256
                     /locus_tag="VW343_000595"
     CDS             43261..44256
                     /locus_tag="VW343_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9755196.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVRKSSVTKELKEKILHYHNQ
                     KFSPEMMVMVKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKSAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGIKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            44282..44926
                     /locus_tag="VW343_000596"
     CDS             44282..44926
                     /locus_tag="VW343_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9755197.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(44968..46956)
                     /locus_tag="VW343_000597"
     CDS             complement(44968..46956)
                     /locus_tag="VW343_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755198.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(46958..47716)
                     /locus_tag="VW343_000598"
     CDS             complement(46958..47716)
                     /locus_tag="VW343_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755199.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            48144..48335
                     /locus_tag="VW343_000599"
     CDS             48144..48335
                     /locus_tag="VW343_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755200.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(48689..48961)
                     /locus_tag="VW343_000600"
     CDS             complement(48689..48961)
                     /locus_tag="VW343_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9755201.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(49019..49468)
                     /locus_tag="VW343_000601"
     CDS             complement(49019..49468)
                     /locus_tag="VW343_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9755202.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            complement(49455..51584)
                     /locus_tag="VW343_000602"
     CDS             complement(49455..51584)
                     /locus_tag="VW343_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9755203.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            complement(51686..51931)
                     /locus_tag="VW343_000603"
     CDS             complement(51686..51931)
                     /locus_tag="VW343_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEU9755204.1"
                     /translation="MNWNNLYFKFDNKDSASEEILNKMDNLPYSNKLILVNRPYKNLK
                     SQCVIPGQEHLPELAIMSDPLNTFDIMAWLKKGGNAL"
BASE COUNT        15171 a        11624 c         9529 g        15636 t
ORIGIN      
        1 gcttgactat gagaatcgta gacccattct ttttcagcta ttttcccatc aaatttgaga
       61 taaaaccaag attccttatc ccaaatccat tcattggctg ccatgtagcc accagatttt
      121 agataataat agtgaccatt ctcgaaaatc cattctttat cagcatagtt tccattttct
      181 tttatgtaaa accaagattg gtattgtttg tcaaaaagcc aaccttgctg tactttggca
      241 ccactagcat tcacataagc ttgattaatc cactgacttg ttagtagata accaccggat
      301 ttgaaataat agtccttccc tttaatttgg agccattctt tctctgcgtg ctgtccatct
      361 gctttgatat aaaaccaagc atcgtattga gaatcataga cccagtcttc tattactttg
      421 gcacctgttg caccaacata ggaagttcct acccaagcat ttcttttcat ctttccatct
      481 tggtcaagat aataaaaggc tcccttgtct tctacccatt ctgatttggc catatagcca
      541 ccagatttga ggtaaaaata gtcgtcacct tgctttagcc attcattttc agcatagtta
      601 gcatctgctt ttatatagaa ccaagattga taatgagtat caaaaaccca ctcattcgct
      661 gcttgactgc catcttcttt cagatactgt tttccttgcc aagtaccatc actagccatt
      721 gcaccctctg gactagctaa gaaaaatagc agagctaaaa aaataaatct tactttcttc
      781 attaaccttc ttcctctgtt cttatttatt ttattgtacc aaaattctcc taaattcata
      841 ttacaaacta ataaaaaatc aagaacagat tgattgcagt ttacctcaga gactctttta
      901 cttcctgacg gagttcttcc agactgctaa taccgtattt atccatgact tttggtaaat
      961 tttcgatgat gtcagggcag gcataaggat tggtaaagtt agctgttcca actccgatag
     1021 cagatgcccc agccagatac atttctaggg cagcttcagc cgaatccact ccccccattc
     1081 caatgatagg caggtctgtt gtttgggcta cttggcggat gagtttgagg gctactggaa
     1141 agactgctgg acctgacatt ccacctgttc cattggccaa gattggtttt ctggttttga
     1201 ggtcaaagcg cattccaacc agagtattga tcatggtcaa gccacttgct cccgcatctt
     1261 ctgcagcttt tgcgacagta acgatatcgg tcacactcgg ggttaatttg acataaactg
     1321 gcacttctga ggcttccaca gctgctttca ccacatcata agccaaatct ggatcttgac
     1381 caatcaaaag tccatgatta cagtggtcaa cattgggaca agaaatattg agctcgatag
     1441 cttttacatt agttgccttg gaaatcccat gagaaacagc tgcatactct tgttttgaaa
     1501 aaccagctac attggcaata ataggaagat ttggatattc tctttccagc caaggtagct
     1561 tttcagccaa aacaacctct aaaccaggat tttgcaagcc aattgcattg agcataccag
     1621 caggcgtctc tgctactctt ggagttgggt tcccaaaacg tggttcaagg gttgtcgcct
     1681 tgatcataat agaacctaaa aggtttaaat catagtactt ggcatactct tgtccaaagc
     1741 caaaacaacc tgatgctgga ataatcggat ttttcaaatc caaaccaggt agagacactt
     1801 gtaatcgatt tgtagtcata attttctcct tataatacaa ctgttcctgt gcggaaaaca
     1861 ggaccatctt cacagacgcg ttggttgacc gtctcgcttt ctggtacttt tagaacacag
     1921 gcatagcaag ctcccatccc acaagccata cgagattcca gagataaata ggctcttggg
     1981 tgatcatcaa aggtttgatt gatatacttc atcattcctg gagccccaca cgagtaaaca
     2041 gcatcaaact gactatctaa atcattgata acaacggaaa catttccctt gatgccataa
     2101 gaaccatcat ctgtcgttac aaagacctga ccatactgag ccaattccgt tttcaaaata
     2161 acagcatcct tattagcaaa accgaggact gtcactactt tcactccacg ttcatgcaat
     2221 tccttggcca cctcaagcaa gggtggaaca ccaatcccac caccaacaag gagaacctga
     2281 ttctgctcat caaggtcaga caagtcaaaa ccatttccct gaggccccat cacatcaaga
     2341 gtgtctccct gacttaaggt tgaaaaaatt gcagtcccag ctccctcaat ccgataaatg
     2401 aggtgacact gcttgtttgc cttgtcaata gacgaaattg aaataggacg acgtaaaaga
     2461 tgggcatcgt ctggtacacg cagatgaaga aattggcctg ctcgcatggc ttcaaccatt
     2521 tctccttcta ggactaattc aaagattgct ggcgcgattt cctcttgtgc aaccaccttc
     2581 atggtttcca accgaatgac acccaaacgc ttcttacatg tgggattcat aacttttcct
     2641 ccttaaattt taaggggacc agataaagaa aagaccttgc tagtgcaagg cctcagttaa
     2701 aatcgtacaa cacaagagag cacactcaaa aagtctcctc tagaaaattg cactattctt
     2761 ttatctgaca ccttgtgagt ctctctggac tccccttaaa ggttgaattt ttattagtat
     2821 actctttcag ctaaaaaaag tcaagtaaaa aacgaatatt ctacttaact tgcacgaaat
     2881 tatttttcac gaatgacttc gaccttatat ccatcagggt ctttgacaaa gtaatagttt
     2941 ggtgtagttc ctggtagacc atttggctca gtcacttcat agccttttgc actgtgttct
     3001 tgatgaagtg cctcaagatc aggtgtactg agggcgatat gggcaaaccc atcaccaacc
     3061 acatacggac cgtgatcgta gttataagtc aactccaact catagtcatc accctcaaga
     3121 cctagataga caatcgtgaa ggcatggtct ggaaaatctc tgcgacgcaa ttctttaaaa
     3181 ccaaaagcat cttgataaaa tgcgattgat ttttcaagat tttctactcg taagcaagtg
     3241 tgtagcattt ttgaagccat atttttctcc tttattttta aaaagactgg acaatcctgt
     3301 tccagtctca tcagttgtta tttaccaagt tttgctttag ctgcatctgc aagagctgtg
     3361 aaagctactg catcgttaac agccaagtca gcaagcattt tacggttaac ttcgatctca
     3421 gccaatttca aaccatgcat caattgtgag tatgaaagtc cgttcatacg agctgccgca
     3481 ttgatacgag tgatccacaa tttgcggaag tcacgttttt tctgacgacg gtcacggtat
     3541 gcatagtagt aagagttcat tacttgttct tttgcagtac ggaacaagat gtgtttagct
     3601 ccatagtaac cttttgctaa tttaagaata cgtttacgac gtttgcgtga tacaacgcca
     3661 cctttaacac gtgccatgtt ttatttcctc caaatatttc ctagaattgt ttacttacag
     3721 tcaagctatt atttcaagcg agtaagcatt gctttgatac gtttgtaatc tcctgaatgc
     3781 accatagatg ctttacgaag atgacgacgt tgtttcttag tttttccgtg gaaacggtga
     3841 gaagtgtaag cacggaaacg tttaagtcca ccagaacctg tacgtttgaa acgtttagct
     3901 gatgcgcggt gtgttttttg ttttggcata attttttctc ctttatttaa ctttctgaca
     3961 attatttttt gtcagtcgct ggcgccaatt gcatgaacat ttgacgtcca tccattttag
     4021 cacgttgttc gatgatggca atatcttgag ttgcttcagc aaactcggct aaaacttttg
     4081 caccaatctc tttatgggta atcatacgac ccttaaagcg aatagatacc ttaactttat
     4141 ttcctttttc aaggaactta cgtgcattac gaagttttgt atcaaagtca cccttgtcaa
     4201 tagttggact tagacgaact tctttcacag taacaacgct ttgtttttta cgttgttctt
     4261 tttgcttctt ctggtactca aatttgaact taccgtagtc cataattttt gcaacaggcg
     4321 gtttggcttg gggttgaatc aatactaggt caacattagc gttatcagcc aaagcttgcg
     4381 cttcactgag tggcttgatg cctagctgtt ctccttcaag accaatcaag cgaacttcac
     4441 gtacacgaat ctcatcattg atgaataagt cttgctttgc tatggttttc acctcttttg
     4501 ttttattaga gaaaaacaat agcggactcg taaatataca agcccgcacg ttatgatagc
     4561 gtttcttcga aacttttcat ccgtagggcc aggcaactta atgtcacaag gcgagaagct
     4621 ctcacttctg cttttctcaa cttttatatg ataccaagtt ttctagctct tgtcaagata
     4681 aaaagttatt ttttgaaaag tttgtgtttg tattttggtt tggttttcga atcaaaaaga
     4741 gagaatcaag ttgattccct ctctatgtta gtttttactt attttcccta cctataggaa
     4801 agctagaatc tattaaaaaa ttcttttttt ctgtgaactt ccccatcttt caataaatag
     4861 aattcccaat agcaaaaaga taatcaggca aggaagtaag accattccca tgctccaatt
     4921 tagatcgaca ttatcaatct gcttaggtaa tcttccagat aaaatctcca ctgaacgcaa
     4981 gtaagtaaag ggaatcagat gtgcaatcct ttgaagaggc tgaattgttt ggatgccaaa
     5041 caataagcca acaatcccaa tgagtgaaag aaagaggaca ggcatttttt gcttgaaaaa
     5101 gtaagcaatc aagtacacaa cttccacaat gacgataaag gctaagaaag ctaagagcaa
     5161 gccaggaaat aatacatctt gtattttccc aatagttact tcttgattca ctaagctata
     5221 aattgggtag ggataatcta actgtccaaa accacttatc agacttccca ctagaaaaga
     5281 aaagccacag attccgataa acagcacagt tacatatccc actccaactc caagagagga
     5341 tattgcaaat gtcacttttg aaacaggata taagtgagct gtgtccagat gattttgata
     5401 tctttctgca aatagttgtg ttagcataaa aataatcgca accacaaaca agcttgggat
     5461 gataacctct aaaatccaga caatctgatc aatcccgtgg gtcggaaact ccaaagtatg
     5521 tgcttttatg ttcaagggat acagggcttg gtaaatcttc cgttcgcggt caacccccat
     5581 ttttaagcca gagctagcag tcgggtcatt tgatacaaat tcataattct tctcttcatc
     5641 tttccactgc agatagtagg cttctttcca gcgcccttct tttagtaaag ttagaatttc
     5701 tgtctttcgc gtcaaaagat ttttttgcac gtctaaatta tttttagcaa actggtattc
     5761 ctccgagctg gtatcagaca tttgggagag tttctcttca ttttcattga tagccctctc
     5821 gttggctgca atgcgacttt ccaacctgct ctccaagctg tgtgagtttg cagtctgact
     5881 atttaaataa aaggtaacac cgagtacaga tacaaataaa gctaagataa tccagtttaa
     5941 acgacttttg aaaacttttt tcaataaaaa tagactaaca tctttcataa actaaacctc
     6001 ttctatctgc ccctgatgaa tggttactac tctatcgcag atatcaacca actcttcctt
     6061 atagtgggaa cttaaaagaa ccagctgttc ttgtctatcg atttgtgcta gcctatcaaa
     6121 aaacttctgt cgataatact cgtctaagcc atttgtaatc tcatccatga gccagaattt
     6181 ggcctgactg agaaaataca tagcaatcac caagcgttgc ttcatcccta aggaatactt
     6241 gcggatggga agactgatat agtcagtcat ttcccagtag gcgatttcat ctctcaagtt
     6301 taggtctgac ttccagatgt tttttatgag acgaaggtag tccatcccac ttaagtttcc
     6361 atccagccat tcaacgctct cataataaaa caaagaagga ggaactgcga tgtgtccact
     6421 actaagggga agcaacttgc tcatagctcg gaatagtgtc gtctttcccg agccattgat
     6481 agcaagaagg ccataaatcc tacccttttt aaaggtaaaa tccgcatctt gcaagatgac
     6541 ttgtcgcgtt tttaaggtaa catgagtaag atttaacata tccagccctc cttttctcac
     6601 tctttaagga ttaataactt ccagtatagt agtttatgac ctcataacga gcgtagttcc
     6661 agcctccgcc aactttatac tcagaatagc tgtaataacg agaccattcc ggaatccaag
     6721 catactgatg gtcgtgatag ttggtactat attccaaaac cgtattccaa tcatacttgt
     6781 aacttttagt gactgtcaca gcagatacac tggactgaag aagaccaata gattataaac
     6841 taactaacta aaacaacttt tgctgatttt taatgatttt atatcctcaa tattgaatac
     6901 gttttcatta tgccgttttt atcattatta tgcaacaaat ttttagttta tttcactaat
     6961 ctacaacatt tatccttcct atcgaacact ttcgattttc caactatcca accccttaaa
     7021 acgtatagct ccttgctggt cagttcgata aactttgcta ttgatacctt ccagtcgtgt
     7081 caatgtttcc tgatggggga gtttcattcg attgctcttt ccaactgaga taagagtaag
     7141 ctctggtttg agtttttcta gaaaggctgg acttgatgat tttttattgc catgttggct
     7201 agctttcaaa acatttactt tcaagtctgg atagtgcttc agcaagtcct tctctccttt
     7261 ctcctccaaa tttcccgtga agagaaattg cttatccaag aatttcccat acagaactag
     7321 ggtatcatcg tgtcctccat ctcccatttt ccttggagat agaacttcta actgacttcc
     7381 aaaaatgggc aagttctccc ctactatcat actacgcacc tttgtttgag tcgcctgtag
     7441 ttctgccaca aattccttct gtttcagact gtcttttgat actagaatct cccctacatg
     7501 gaaagccttg gtcatctctg acaaatctcc aacatgctcc ttgtccgtgt tagtcaaaat
     7561 tagctggtca atcttagcta ctcctcgact tttgagatag ggtatcaagc ttcgctgggc
     7621 attgctggtc gtcatctttt cttgccattt tttgattttc ttataagatt ctgccttacc
     7681 acctacatct atgagaatgg ttttcccagt tacatcccgt aggaaaatac tttctccttg
     7741 ccccacatcc agcatggtga tttcattttc cagtggatgc ttggtcagga gaaagagccc
     7801 tgtaatcaat aagcacaata ccgttagctt tttaatgttt ttcctcaaat catagaccaa
     7861 agccaaagaa attaacaata ggattaaaag ccattcattg ggttgtccaa agacaagcgg
     7921 tctacttgcc acctgcgaga ccaagcgaat aatgccctct aaccattcaa agataaagtt
     7981 cagctgaatg actggataga gaaaggaaag gacaaataag atagacaaga gcggtaagaa
     8041 gaccaagtca aaaagaaagg aaaagacaaa ggtcaaaagg atagaccaag gttgaaattc
     8101 cgcaaaatag aaggatagaa tgggcaatat gcccaaggag atgactagac tttcactagt
     8161 aacagccttg agcccctccc cttctttgct ggtcatggtc aggataaaag cataagcaca
     8221 ggacaagact cctcctgctg tcaagaaaaa gtttggcatg acaataaaga ggacaagcac
     8281 cgtcaaggca aaattatcca agcccttaac cccatgttga gccagtagct tttgcaagag
     8341 actgcgaata accgatgctg aaaatccagt tagtcccgca tagataaggg aaaagggata
     8401 agtcagccac ttcaactttt cttgtgtcaa gcccaatcgt aaaagtagtt tcttaaatcc
     8461 gtccatgaaa aaccctacct gcatacctga caaggcaaaa aggtgaataa ttcctaaact
     8521 ggaataaagc tcattcatct cctcaaaatc ggtgtccaga tgtcctagca agagtcctgt
     8581 catgtaattg cgcatagggt ctgggaagtg catcttaatc caaaccacag cctttcgacg
     8641 taaactggac aggttttcac ctatatccca actgccaacc ttttggagtg actggattct
     8701 tttaatattg agtgtctggt aaattccctg agtcttcaga tagccttggt agtcaaagcc
     8761 accaaaattt ctctgccctt ctggctccga aagcttccct tctagtccta tctcatgaag
     8821 gtcagttaaa gcttgaaagg cttctttctc ctcctcggac tggagtttat aatagacttg
     8881 gaaagcacga ccatcagact taccacgaaa ggacagactg tcaccgttaa ccttaatagt
     8941 gtctggtaaa atccgtaccc tttcaacaga atccaccaaa ttttgactca cttgactctg
     9001 ttgccaagtt tgaaacagaa accagaagcc aaagactcca caaatcacta gaactttacc
     9061 tgctgatttc caaggaaatt ggataaagag gcagactagc agaaaaacaa aacccaacaa
     9121 agcaaaatag gatactgaga aaatggcgta gtaaagccat agtaacagaa aactcaggta
     9181 aattagggga atagagaaat tcttaatcca ctgtaacata gtctttaagt ttttctatgg
     9241 tcttggcacc aatgccagag accttcttga gctcgtctac tgacttgaac ttgccatttg
     9301 cctcacgatg gtcaataatg tcctgagctc gttttcctcc cagtcccttg acctgcttga
     9361 gttcttccag actggccttg ttgagattga ccttcttttc cttgcttgtt gaagaagctg
     9421 tccccgaacc agtctgttga ctaactgctt cttctccctt agtaggaacg taaaccagag
     9481 cctcatcact aactttctga gctagattga gcgacttgct gtctgcttgc tctgtcaagc
     9541 caccagcctt ctgaacagca tcattgactc gactacctac aggcaagtca taaatccctg
     9601 gcgatttgac agcacctttg acatctactg tgattagatc ttgttcaact ggttcttcct
     9661 tctcttcctt gttcacttcc tttccggtcg atgagtcctt ggaaacagct gcaacttcag
     9721 cctgcaaatt cgtctctttg acagatgttt gtggagctgg ctttagcagg aaaaatcctc
     9781 ctacaagcaa gcccagacca gtacagatga cgatgatttt atactctttg attttctcga
     9841 taattgcttc catattttct cctctcttag attattcgta agaggaagaa aaaacagtcg
     9901 aaaaatttct tttcaactgc ttatttattt gcaaaatagt cttccttggt caagacataa
     9961 tgaactcttg tcacgattcg gcctttttca tgctggtcca tacaagcata tggttctgca
    10021 tgggaaaaac gcatgcctga tttctccatg acctttcctg acgcgggatt agccttatcg
    10081 tgaagggcag tcaacttatt catccctatc ttctcaaaag ctagctcaat cacagcacga
    10141 ttggcttctg tcgttaatcc ttgattccaa tactttttat tgataatgta gccaatagct
    10201 gccttcttaa gaacaggatc aatcttgtgt aagtcaatag ttccgataaa cttgccgtta
    10261 tcttttaatt caatccccca acgtcccaag ggattagcca agtagaactg agcaatgtta
    10321 ttcttggttt cttccaagct ttgattggtt ggaaaagtgt aacgtgtatt acccttgtcc
    10381 gaggcatagt caaacattgc ttccgcatca tccaaagtta caggtctaag caataaacgc
    10441 tccgtttcta tagacggata ctgggcaaat ttcacaaata ttgattccat acttttccct
    10501 ccattagagc ttgattttca ctagtctagc ataaaaaaat ttggatgaca agtttttctt
    10561 gatttttgaa gcggtttcat atacaatgaa accgtcacat ttttgattta tgaactggaa
    10621 tgtgacaacc aaaacaagac cgtcaattga aaggtaggtt atctcaatgt taatcggaat
    10681 cccaaaagaa atcccaaatt tcaagttgaa gttagccacc caaaagaaat tcacttggcg
    10741 acttgtagtc caagcatttt ttagggtagt tgttaatcca attttcgata aaagctactt
    10801 ctttcggagt cgttttcttg gttcctttag gtaaccatct ccgaattaat cgattatgat
    10861 tttcatttga acctctctcc catgaagagt aagcatgtgc gtagtagata tgttcctcag
    10921 gaaatacctc agacaatcgt ttgaactccg aaccattatc tgcagtaatg gacagaatac
    10981 gatgctcccc taatagtaac gtaagcgcct gattgacaga ttcagcagtt ttatttggaa
    11041 ttaaccttat aatttggtgt ctgctccgcc ggtcggttaa gactaacagg caataatttt
    11101 ttatcttagt cagtaggacg gtatcaattt cataatgacc attttccaag cgaagattaa
    11161 taacgtcagg accttcttcg atagatttac ctgccggctt aaagttcgga ctagcttgct
    11221 tcttgacacc tttccttttt ctgggataaa gcatgtcggc cttcgtcaat cctaaatgac
    11281 cattatgaaa ccagtagtag atggttgaaa taccaacttt cacttgcttc ttgttaacca
    11341 tcatttcagg cgaaaatttt tgcttatgat agtgtaagat cttctctctg atttcctttg
    11401 ttaaaattaa ctttttcacc gaccgttttc gattgaattg gtaaacagtt tgtgcgtaat
    11461 cggcagaata gacctttttg tatagccctt ttctcacttg ttgtaaagtt gtacctcttt
    11521 tgacttcatt atgaatcgtt tgaggcgcct ttcctaacaa gcctgcaatt tcacgattgg
    11581 acttattttc attcttccac cgctcaatca agcgacggtt atctattgtc aaatgtttcc
    11641 cttttggggt ataatgttct tgcatctctg tgcctttcct tgtgtttgtg gtgaacaaca
    11701 agtatagcac agaggtgttt tcttatacct taaatcacat ttcatttgac aacaggaacc
    11761 cttgaacctg ttttttcagc taaaccaagg ctatgattag ccttggccac ctagcctaac
    11821 agtttcaaac ctgttatcaa atgaaatcat caacttactc aaacatagtg aaacatctaa
    11881 ttcttgacta aggtaaggtg gctaacttca ttatagaact ttcaatccca aaagaaatta
    11941 aaaataacga aaaccgtgtc gccctcacac ctgcaggtgt tcatagctta gttagtcgtg
    12001 gtcatcgtgt ccttatcgaa acaaatgctg gtctcggttc tggctttact gatgctgact
    12061 atcaaaagca aggagctgag attgtcgcta ctgctggtga agcttgggca gcagagttgg
    12121 ttgtgaaagt aaaagaacct ttaagttctg aatacggtta cttgcgcgac gttcttcttc
    12181 tcttcaccta cttgcacatg gccgctgctc cagaattagc agatgctatg ttagcagcaa
    12241 aaataacagg aattgcctat gaaactgttc gtgacaatca aggacaacta ccgctcctcg
    12301 ttcctatgag tgaggttgca ggtcgtatgg ctgtttaaat cggagctcac ttccttacta
    12361 agcaagctgg tggctctggt gttctacctg gtggtgtacc aggtgttcca aaaggaaaag
    12421 taactatcat cggtggtggt gtcgtcggta cacatgctgc ccgcatcgcc cttggtcttg
    12481 gtgctcaagt gactatttta gatattagtg ccaagcgtct ctcagttcta gaagaagtct
    12541 ttggaagtca aattcaaact cttatatcta attcattcaa cattgaagca agtatgagag
    12601 atgctgatgt ggtgattgga gccattctca tccctggtgc aaaagcaccg gaattggtga
    12661 cagatgagat ggtcaaacaa atgcgtccag gctctgtatc gttgacgttg ctgttgacca
    12721 aggtggcgtt atcgaaacag ctgaccgtgt gacaacgcac gatgaacccg tctatgaaaa
    12781 acacggtgtt ctccactatg ccgttgccaa tatccctggt gcggttgctc gcacttcaac
    12841 catcgcccta accaatgtca ctcttcctta tatcgaagct ttggctggca aaggattcgc
    12901 acaagcaatc tctgaagatg aaggcttgcg tcaaggtgtg accacttatc aaggttactt
    12961 gactagccta ccagttgctc aaggacttaa tcgtgactac actgatatca atgatttagt
    13021 ataaattttg aaaagaaaag ggtgacttcg caagtcctac ccactgtgct ttgtgtcatc
    13081 agctattaca tcgttggttt attgtactgt atactttgca actggagact ttcgttccag
    13141 actcaattgt gcataagaaa ctctaaattg aataaagtca acagagtttc gcccaaccac
    13201 ttcgtcttgt aatgacaatt atataaacca aaggaagctg agctctttag tcccagcttc
    13261 tttttatata taaaatttta cccgtgcaaa gacagggcct tagcagactt cttttttact
    13321 tcgttcaccc ttgctttttc tttttaggtt tgggcgttgg cagttggtta tacatagcta
    13381 aaatcaggtc ttatagaaac atcttattat caagttcttc cactcaaatc atttctttgg
    13441 cacctttgta tggaaactca aaaaaagatt ggtcaatctt atctaagact gcttgcacgg
    13501 gtttaactaa aaagcgatca tcataaatgc cgccaataat cttgccgcgg aagtaaagaa
    13561 tatactcccc catcatggaa cggtaagtca catcatctaa tcctgataat tgttccaaaa
    13621 caaattccaa atagttctta cttgatgcca tttctaatct tctaggctct gttcagcgat
    13681 aacaaccgta tagagttctt gcttaacctc gcatccaatt gatttaaagc cctgcttttc
    13741 ccaaaaatgc tgagattgcg gatttccctt aacataagcc aaacgtgcct ttcgaaagtt
    13801 cttagcaaaa taagctagtg cttctgtcac aatatgacta ccaatccctt tcctctgata
    13861 ggcttgatca accataaaca aaccaataaa aacagtctcc tcatcaggat gtgcatagac
    13921 aaaatctata acagccacaa ggtcagatcc attccaaaat ccaacaaaaa acttatcagc
    13981 cttagcttta ccttcaggta gacaaagcat gtcctctttt acagttgcaa aatttggctc
    14041 tggtggacaa tgctgaaaat acagaggatt actttcatat aaagataaaa tacttggaat
    14101 atccttttca gttagtatcc tacaactgta atacttagat agttggtcaa tcatcttttc
    14161 aaattcctac tttcttgtgc cctgtgatta tgacacagga agatgcactg atcgtcatca
    14221 gccacataaa agttctttcc atcgtgccta atcgttgtct caaacctttg gataaaacct
    14281 ttagcctata caactggatt ttcctctctc aaaagtatat tcttttgcag gcaaacttcc
    14341 tcaaaatcag tcgtgtgcaa cttcagtaga atattcatag gctcggataa tctgagcgac
    14401 aactggatgg cgaaccacat ccttggctga aaaatgaaca aagtcaatct gatggatgtt
    14461 cttgagtttc tcttgagcat caatcaaacc ggacttgaca ttacgtggca ggtcaatctg
    14521 actaatatct ccattgacaa tcatcttaga atgaaaacct aaacgagtca agaacatctt
    14581 catctgcatg atggtcgtat tttgcgcctc atcgagaatg acaaaggcat catccaaggt
    14641 ccgtccacgc atataggcaa ggggcgcaat ctcgataatt tcacgctcca tgagtcgagt
    14701 tgtttggtct ttccccagaa tctgatacaa ggcatcgtaa acaggacgaa ggtaaggatc
    14761 caccttctcc ttaagatcac ccggaagaaa tccaagactc tctcccgctt ccaccgctgg
    14821 acgagttagg ataattcgct tgacttgccc acgtttaagg gcagtcactg ccaaggtcac
    14881 tgcaaggaag gtcttccctg tacctgctgg cccaattcca aaggtcacat catgctgttt
    14941 gacactgtcc acataaagct tttgccctag ggttttgaca cggataggtt tcccagtatt
    15001 atctttgata atttcttctt cgtaaagggc gacaaacttg tcaatttcat catttttgac
    15061 catgctaatc gcagtgacta catctggcgt accaacggtc atcccacgat ttaccaagac
    15121 catcaaagcc tgaataactt gacgggcttc ctcacaggca gactcttctc ccaaaacctg
    15181 gacaatctcc gtacgagcat gaatcacaac atcaagctct tcttccatca aacgaagatg
    15241 gcgttcattg gaaccaaaaa gatgaaacag gtcatctgga tgactcagtt gaatgtctat
    15301 tgaatgttcc ttcaaataaa gaacctctct aatcctttta tttcttttta ttatagcaga
    15361 gggatggaga aattactagc ccaaacccaa tgaataaatg ctagtgttct agatattcct
    15421 gccaaatgct gtcaaaatct cctaggttaa aagagaaact ggcatgctcc tccaaaaagc
    15481 gactgacctc atcaaaatca tctgtatgtt ttggaaaagc tgcctcttca aaagcgaggt
    15541 ctgccaaaat tgctttggga ctgttacttt taggattgcg ctcggtcatg agccaagtgt
    15601 aaaatgattt tctcataagc ctccctaaat caactctgtc ccaaactggt cctgcttgat
    15661 tttaccagcc ttcataagac cacctaaagc tttcttgaac tgacctttag aaatgccaaa
    15721 ggttgccttg atgtcgtctg gagatgactt atcatttaag gtcatgaaac cgccattact
    15781 ttccaaataa gtcaaaatca tctgagcatc gttttccaac atttcaaagg agcgtggttt
    15841 gagggagagg ttcagagtgc ggtccacttc acggaaacca ataacgcgcg catctaatac
    15901 ttgccccaaa cgtggctctg cgtaacgctc gctaggatga ataaaaccaa gcatattatt
    15961 ttctggtagg taaacaaaag ttcctgacag cttgagacgg taaacaatgg ctggccagtt
    16021 ttggttctgc atgttgttgt aggcaggacg agcaagacgt tggaagtctt cttgataagc
    16081 caagaggccc cagatacggt ctttcttatc cacttcaaga cggatgtaga gttggtcgcc
    16141 cttcttaggc cagagttcct tgagctcagg gagaatatcg agtgacacaa cgatttcctt
    16201 gtcaggaagg cctgtatcta caaagacacc caagtcctta cgaacctctg tgacacatcc
    16261 ccaaccaaat tggtcctgag tggcagtcac ttctaaggtt gtcaggcgga gtttttgctt
    16321 catatccgtg tatgcaaaac ctttgaccgt atcccctact gtatgttggc cctcttcctt
    16381 agcaagagca taggtttgac catccttttg cacaaagtaa aaacggtcat tttcatcgat
    16441 gatcagtcca acgataaaac ttgcaagatt tgtattcata tttccttctt tcgaataaaa
    16501 ctcagccagc aatgccaact gagtttttct gtttattttt agacttccaa aagttctttc
    16561 tctttgttag cagtcatgtc gtcgatgtgt ttaacagcat cgtctgttac tttttgaata
    16621 tctttttcaa gagtcttcaa ttcgtcttca gtgatttctt ttgctttttc ttgtttctta
    16681 gcttcgtcca tagcatcgcg acggatattg cggacagcca ctttagcatt ttcgccgacc
    16741 ttcttcactt ctttagcaag gtcacgacga gtttcttctg taagagctgg gataaccaag
    16801 cgaatcacag aaccgtcatt agccggtgtg ataccaagat cagaagcgtt caaggcacgt
    16861 tcgatgtctt tcaatgaaga cttgtcaaat ggtgttacca acaaaacacg cgcttctgga
    16921 atcgtaattg aagcgatttg gttaagagga gtttcgactc catagtattc tacatgtaca
    16981 cggtcaagca agcttgcatt ggcacgacca gcacggatac caccaaattc acgagcaagt
    17041 gattggtgag attgggtcat tctctcttta gctttttcaa taattacgtt agccatattc
    17101 tttcttattc cttttcttcg atattatttg aaactgttgt tccgatattt tcaccaaata
    17161 cgacacgttt gatgttgcct gattggttca tgttgaagac aaccaagtca atgtcgttgt
    17221 ccattgagag ggttgaggct gttgagtcca tgatacgaag acctttgttg ataacatcac
    17281 ggtgggtcaa ttcttcaaac ttaacggctg tcttgtcctt cttaggatcg gcattgtaca
    17341 caccatcgac gccattttta gccatgagga tggcatctgc ttcgatttca gctgcacgaa
    17401 gggccgctgt tgtatctgtc gagaagtatg gtgaaccaat tccagcacca aagataacga
    17461 tacggccttt ttcaaggtga cgaagggcac gtccacggac ataaggctct gccacttgtt
    17521 gcatagcaat agctgtttgt acacgcgtat caaccccaac ttgttgcaat gaatctgcca
    17581 tcacaagagc attcataaca gtcccaagca ttccagtgta atctgcctga acacggtcca
    17641 tacctgcttc tgctgcaggt tctccacgcc aaagatttcc tccaccaata acaagggcaa
    17701 tttcgatacc taagctatga acttcttgaa tctcttttgc gattgtttga actgtttgga
    17761 tatcaatccc tacgccacgt tcaccggcaa gggcttcacc tgataacttg attaaaatac
    17821 gtttatactt gggattcgcc attttcactc tccttctttc atcctaccta ttttatcaca
    17881 atttctaaga tttttatagt atcatgagca attctttcaa aaaaattaga cagtcaaaaa
    17941 ttcctctaag tcggcaaggg cacgctctgc aattttttca taacgagcct tcttatcacg
    18001 gatacgctcg ccttccaact ccttgatgat cccaaaattg acattcattg gttggaaatg
    18061 tttgctgtcg gcatgggtaa tgtaatgagc taagcttcca atcgctgtcg tctcggggaa
    18121 aataacctcg ctttcttcct tgaagagacg agctgcgtta attcccgcaa ctaagcccga
    18181 agccgccgac tcaacatagc cttccacccc cgtcatctga ccagcaaaga agagatttgg
    18241 ttgtttcttg gaacggtaag tctgctcaag aagatttggt gaatccatgt aagaattgcg
    18301 atgcatgaca ccataacgga caaactccgc attttcaaga cccggaatca tttggaagac
    18361 acgcttttgt tctccccatt tgaggtgggt ctggaaacca acaatattgt agaggctacc
    18421 agctgcatta tcctgacgaa gttgcacaac cgcataaggt gttttaaatt ctccatcacg
    18481 aggtcctgtg tagtcgtccg gatactcaag accgactggc ttcatagggc cataaagcat
    18541 agttttaatg ccacgtttgg ccataacttc gattggcata catccttcaa agtacttttc
    18601 tttttcaaaa gaattgagcg gtgcttcttc cgcattgacc aaggcttcat ggaaatccat
    18661 aaactcttgc ttggtcattg gagcattgag ataggctgct tctcccttat cataacgaga
    18721 tttgagatag accttactca tatcgatagt gttgacatcg ataataggcg ctgctgcatc
    18781 gtagaaatag aagccatcgc cattattaag ggcatgaatc ttttcagcca gggcatcgct
    18841 agtcaaggga ccagtcgcca caactgtaat aacatctgtt ggcaattctg taatttcatc
    18901 acgcaccact tcaatcaagg ggtggttgac cactttttcg gtcaccattt gagagaaacc
    18961 atcacgatcc accgcaaggg ctccaccggc aggaacacgt gtagcctcag cagattccaa
    19021 gataacagaa cccaagcgac gcatttcttc cttgagaaga ccaactgcat ttgtcaaagc
    19081 atccccacgc aaagaattgg aacaaaccaa ctcagcaaaa ttgtctgttt tgtgctgagg
    19141 agtcgacttg acaccacgca tttcataaag tttaactgga ataccacgct ctgcgatttg
    19201 gtaggctgct tcagaacctg ccaaaccagc accgataaca ttgatataag attgagacac
    19261 gacactaata cctctttggg agtgtgaagt taagattcac attgaaaaag ccaatcagac
    19321 ttacgagatt tcgagtttct tggctcaggc tgaaaaagtc ccccagactt tcctcgctct
    19381 cgaatttctt ggctcaagct gaaaaagtcc cccggacttt ttcactccca caaatctttc
    19441 tatttttttc ttctactagt ataacaaaaa aagggaagaa ggcaaacttc cctgtttagt
    19501 cattttcttg atgtaaagag atagagagta ttccagttaa gaatcaatta tatgctactc
    19561 tataaaatct tttccacata acggatcgat agggactgtt attctatctc ttgctataac
    19621 cgtattatct aaaacagcat aacattcaac atagtgatct cctttaaact gtgaatcttc
    19681 gtgatatttt attttacctg aaaaaatagc accacgctca caatttcttc taatagcctc
    19741 agcttcaata tttcttactt tccaatagac ttcctgcgaa acaaaatatg gtatagtagt
    19801 tctatgaatg atgaagcaag taaacaacta actgatgcac gatttaagcg tcttgttggt
    19861 gttcagcgca cgacttttga agagatatta gctgtattaa aaacagctta tcaacttaaa
    19921 cacgcaaaag gtggacgaaa acctaaatta agcctagaag accttcttat ggccactctt
    19981 caatatgtgc gagaatatcg aacttatgaa caaattgcgg ctgattttgg tattcacgaa
    20041 agcaacttaa tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag tggtgttacg
    20101 atttcaagaa ctcctctcag ttctgaggac acggtaatga ttgatgcgac ggaagtaaaa
    20161 atcaatcgcc ctaaaaaaac aattagcgaa tcattctggt aaaaagaaat ttcacgctat
    20221 gaaggctcaa gcgattgtca caagtcaagg gagaattgtt tctttggata tcgctgtgaa
    20281 ctattgtcgt gatatgaagt tgttcaaaat gagtcgcaga aatatcggac aagctggtaa
    20341 aatcttgtct gacagtggtt atcaagggct catgaagata tatcctcaag tacaaactcc
    20401 acgtaaatcc agcaaactca agccgctaac agctgaagat aaagcctgta accatgcgct
    20461 atccaaggag agaagcaaga ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatgtt
    20521 ttcaacaatc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg
    20581 cattatcaat catgaactag gattctagtt ttgcaggaag tctaatgtaa ttctcaagct
    20641 gtgagttcat tatgatttgt aaatccgata tatcttcagc agctaaaaac acattttgaa
    20701 atgttccaag tccgttttca ccaaaactat attctctctt actatactta ctcttattat
    20761 tctctatata gttagagtca aggttcttaa cctcttgaag ttcattttct ttacttctga
    20821 ggttaaaatc ttttacttct gaagttaagt tttttgactt ctgtaagtca ttttctttta
    20881 cctgaaatat actcataaag tctttaacat aaatgatgtt cggcttacca agtccaagcc
    20941 ttactctttc gatcagtcct attccttttt tacatcaagc tcatctaatg tttttatggc
    21001 agttggcttt gagatatttc ttcttttcat aatttcttcg acagtaaaat agataaatac
    21061 tctgccttcc ttgtctatcc agttattctt aaatgacatt cctgtctgtt taagaagtat
    21121 ggaataaagg ataattgctt ctgctgataa gccccgaaat tcttctctat caactaatat
    21181 ctccggtact tttaagaagt taaatctttc cgcttctctg ttataaaaat attcaaagtc
    21241 catgcaagat cacctccctt cttaaaaatt tgcaaagaaa aagacgatag tttttcttct
    21301 atcgcctttg gtaaccattt ttatgaaatt ttttaaagtt aaaaatataa tttataatac
    21361 tatcttcttc cattttctga ttttagttct gaaagttcca aaaggtgcaa ccgtatttac
    21421 atgaataaac ttatacactt cccaagtcgc tgttttagtc gcttcatcag cccactttct
    21481 catatgcggt tcaaataatt cttcctcact caacgaatca atcattgcag agatagaatt
    21541 aatattttca tttaattttg ctttcaactc ttgcagagat aaatgagcgt aggtatctgt
    21601 gaaccactga tataattcac caagttgatt ccatttaaat ttatccgatg gtgtttttac
    21661 ttgaagcccc tttctttcat cttcttccca tttaagaacc aagttggtcc aaccaacctg
    21721 ataagcaagg ttttctgctg gagttctgtc aacttcatca gctctcttat cttttaaatt
    21781 ttctggaata ttatcaaatt ctaaaatata tttctcaaat gttttctcta tctcagcttt
    21841 tagttcttct ttattttcgt atgttcgcaa aaaatcacct tctttctgat ttatcaatcc
    21901 tttgatttaa aatgacctta tttttctatt tataccttct aagaacgcta taactgctgc
    21961 tccaaccatc ggtattccgt atgggcttaa taaaaaactt aataccaaag cttctatacc
    22021 aagtgataca tctttatatt atcatcacta attaagttaa acagaaacag acagaattat
    22081 tacatcctgt ctccctttat tgtttttctt cccagtcgtg gtaggcagcg tagagctgac
    22141 ttttattcca ctggtaaatc ttagcgactt ccttgatagc ttggtttttc ttcacacctt
    22201 gctggatgcg ggtttgaatt tctacgaaca agtcttcctc gtccttttcc tccacaccct
    22261 gactggcacc ctcaacaatg agaagacatt cgcccttgag tggcgtttca gcaatgcttt
    22321 ctaataactc agagatagta cctcgttggt attcttcata gattttggtc aattccctga
    22381 ccaagacaac ggagcggtca ccgtagactt ctaacatatt ttccaacgtg tctgctacac
    22441 gatgaggtga ttcataaaaa atctgtgttt caggataatc ttttttcaag ccaaaaaatt
    22501 gcttctgctg acctgatttt ctcggtaaaa aaccgtaaaa gatatgtggc tgtggcgcta
    22561 aaccactggc aatcaaggca gaaattcctg cagaggcacc tggaactgtc acaactgcaa
    22621 tttcttcctc aatagctgcc ttaactaaat catgaccagg gtctgaaatg ctaggcaaac
    22681 cggcatcaga gacctgagca atactttgcc ctgctttcaa gaaaccaatc aaatcaggaa
    22741 ttttttcctt ggcattgtgc tcatgaaaac tgatctgctt ggtggaaatg tcaaaatgct
    22801 tgagcaaaag ccctgtattg cgcgtatcct cagcagcaat ccagtccact tctttcaagg
    22861 tctggatagc acgaaaagtc atatcatcta gattgccaat cggcgttgcc actagataca
    22921 gcttgccata gggagactgc cccttaaaac ttttttgaat ttgcatgctt actccctgta
    22981 taacaactca tcacagaaca tacattcttc gtcctgctct cgacgttgtc cataaaaatc
    23041 attacatacg tgaaatccat cacggtaaat acgacggaca ctttcgcgaa catgcttggc
    23101 cttgacggga gcgtctgctt ccacctcacc caagcgttct cgcaacttac tattttccaa
    23161 gcgaagagct gtattttctt ctaccaagct cttgagattt ttcttgatgg cttccacatc
    23221 ggctaaggtt accaataatt gttgggaaaa atcatccagc gcgtcaaata attctttttt
    23281 gtccataaaa cagccctttc ctttctttat cattcatttt tgagtttata tttctttcaa
    23341 gaccagatat tccatggcat tttgaaagct gacattagct tgccacattt ttctagcttc
    23401 tagtaaatct tgtagaatca ctcttactct tacctgcaag aggtcctgcc cacagagaac
    23461 ttcaagaatc cgtaaaacct gatcctgttt ttccttatca tctgccaagt tggctaattt
    23521 ggcaacctgt agataacttt cttttttctt agctactaac caagtcagca ggcgttcact
    23581 ttcatcgacc aaggtccaaa aacttgcctg attagccaac ttttctgctt cagctcgcga
    23641 ttgactaaac ttagctaaaa gagtcgcttt tttcttaaca agtcccattt gttctaagag
    23701 taagataagt ttttcttctt gctttttaaa gtggaagatc tgagtccgac ttcggattgt
    23761 cggtaacatc ttttcctcat cgctagtcaa gaagaaaata taaacttcac tctggggttc
    23821 ttcgatgacc ttgagcagag aattggctgc gttgggatgc attttatccg cttgctcgat
    23881 aataaagacc tgttgctggc tttcaatccc tgcttgagaa aactgtccca ccaattcccg
    23941 aatgcgttct gtcttgatga cctgattgac tggcttaatc aaggtgacat ctggaaactc
    24001 ttcctgttca atcagcttgc aacttcggca tttctcacat ggtaagacgc caactttatc
    24061 cgtacaaaag aggctcttag ctaaaaattg cgccatttcc aagcttccaa agaaacctga
    24121 aaagagatag gcgtgattga gctggtcttg ttctaagata cggacaaaac ggtcaaactg
    24181 agctggttgc caagccttta gttgatcttg tttcatttgg ccaagtccat tccgtcaaac
    24241 aagacagcct tggtagtttc cacaacttgc tccaaaggga gactagcatc aatcttgaca
    24301 atgcgatttc cttctttttc caggagagaa aggtagcctt gacggacttt cttgtgcaat
    24361 tccaaacctt ccaagtccag acgattgacc tcgcggtcac tattcgcagc aatgcgagcc
    24421 agcccttctt ccacctcgat gtcaaaatag agtgtcaaat cgggtttgag gccatctgtc
    24481 gcaaactgat tgagccagtc aatggcttca atatctaagc cacgaccaaa tccctgatag
    24541 gcaacagaac tatcgataaa acgatccatg atgaccaact tgccagcttc aagggctgga
    24601 agaacttttt ccaccaaatg ctgtctgcga ctggcaatat agagaagtag ctctgtttta
    24661 gcatccatct gagtatgact tggatccaaa atcacttccc gaatcttctc cccaatcaag
    24721 actccgccag gttcacgggt cgtcagcacc tttactcctt tttcctctaa aattggtagc
    24781 agagcctcta aaacactggt cttgcctgct ccctctggtc cctcaagaga gactaaaaat
    24841 ccttttgaca tgtctaactc atttcttttt tactacttct attctatcaa aaaaataggg
    24901 gtttgtgaca atctttttgt cttctcattt catactcaat gaaaatcaaa gagcaaacta
    24961 ggaagctagc cgcaggctgt acttgagtac ggcaaggtga agctgacgca gtttgaattt
    25021 gattttcgaa gagtatcaac caccataaaa gaggcagata aaactacctc ttatttacct
    25081 agttcttgtg ttcgtttgta ggcttgatga actgcatcca tgactgttcc tcggaaagca
    25141 tgcgcttcta ggcttgctac accagcgata gtcgaaccgc ctgggctaca gacttggtct
    25201 ttcaatactc caggatgttg ctgactttca aggaccaatt gcccagctcc taccacagtt
    25261 tgtgctgcca ttttcaatgc tatttctcgt ggtaatcctg tctgaacacc tgcatctgcc
    25321 aaggcctcga taaaaagata gacaaaggct ggtccacaac ctgcaagacc tgtcgctgca
    25381 tcgattaaac tttctcctag ttcaaccaag agaccagcct tggctaaaag ctgataaaag
    25441 agctcactgt cctcagccct gcaattagga gacaaggcat aactaatcac tccttgcccg
    25501 atagaagcag gggtattagg catcatacga ataattcggt gttgacttgg gataagactt
    25561 gctagttttt ctaaggtcaa tccagctgcc atcgaaatca aaagaagact ttctcttttt
    25621 tcaaggatgg tctggtattg agaaagcagt tcagaaaact gagcaggctt aactcctaga
    25681 aaaatcacat ctgcttctgc aaacatttct tcattgctgg aagcctgacc accaaagtct
    25741 gcaatgaaag catctacctt agcttgacta cgattggcaa ggagaatctc atctgacgtc
    25801 ctagtctgca agacagattt tgccaagcta gcacccatat tccccaaacc gataaatcca
    25861 atcttcatct cttactccct tatctgccca tcaccggcaa ccacatactt gtagctggtc
    25921 aactctttca agcccatggg accacgcgcg tgcaatttct gagtagaaat ccccatttca
    25981 caaccaagac caaattgtcc tccatctgtg aaacgagttg aggcattaac atacaccgct
    26041 gcagagtcca cttgatctgt aaagtatgct gcagcttcag cattttccgt cacaatagca
    26101 tccgaatgat gggtgctgtg ggattcaatg tgcgcaaccg cttcttctaa actgctcaca
    26161 accttaacag caaggacata gtctaaaaac tcggtgtcaa agtcttgggt ctcagctgct
    26221 tgacctgaaa caaactggct tgctttgcta tccaagcgga attgaattgg ttccagtcca
    26281 gcttccttac gctctgcaac cagcatttga tccaagcgag gaaggatgct tgctgccttg
    26341 ttttcatgaa ccagcagaac ctccatggca ttacaaacag aaggacgact ggttttagca
    26401 ttgttgatga tagacagcgc cttgtcttcg tctgcatcct tatccacata gacatggaca
    26461 atcccagtcc ctgtctcgat aacaggtaca atcgcattct caaccactgc attgatcaag
    26521 ccagctcctc cacgaggaat gagaaggtct agatagccct tggccttcat catagcataa
    26581 ctactttcac ggctagtatc ctccaccagt tgaatcacat ttggatgaat agtagtcgtc
    26641 tccaagccct tcttcaaggc tgtgacaatg gcatgggttg tttgataggc atccttacca
    26701 ctacgaagaa caaccgcatt tccactctta agagtcaaag cagccgcatc agacgtcaca
    26761 tttggacggc tttcatagat aataccgatg acacctatag ctacacgttt ttttgtgata
    26821 accaaaccat tttcaagctg acttgtttct aaaacttcac cgattggatc tggtaaggca
    26881 accacttcac gaattcctct tgccatcgct tctatacgat ctgcatccaa ataaagacga
    26941 tccaacatca catctgagat tttcccctta gccgctgcca tatcgagggc attagccgct
    27001 aaaatttcct cagtagcagc cactaagtga tcagccatgg ctagcaaggc ttggtttttc
    27061 acttcttcac tagctgtgtt aatcgatttt ttaacagcct gtacctgttc aaattgttct
    27121 tgtctactca ccatagttta cctctaaaat tctgtaaaaa gtagttggat ttcaggagta
    27181 atggaaatcc agtcgtcacg gtaaatcaag acacccttgg ctttttgaga acgcaacata
    27241 tcctccaaag cagatgctcc aaattgcacg cgtccttttc caagtgattt tccactttcc
    27301 ttgtcaaata ctgtcacgat atcaccgtaa gaaaaggctc cttctgcttc aacgatacca
    27361 gataagagaa gactctttcc atgttgagag agagcttccg cagccccttt atcaacccaa
    27421 atagaacctt gactctgagc atagaaggca agccattgtt tctgggtacg aagccccttc
    27481 tcttgagcaa caaagtaaga accatcctcg gtctcctctg ccgcctcaat catggaatct
    27541 gatttcaagg atgagcagat ataaacagga actcctgatt ccgtcgcgat agttgcagcc
    27601 ttgattttgg ttaacatacc cccagttccg tttgacgaac cagctccacc agccatatca
    27661 ataatctcac gattgatggt ctcgattctc tccaagcgtt tggctcttgg atctgaatta
    27721 ggatttccag tatagagacc gtccacatct gtcaagaaaa ctaaaaggtc tgcttggacc
    27781 atcgccgcta cttgagcact tagagtgtca ttgtccccaa ccttgagctc atcaataacg
    27841 acactatcat tctcattgat gataggaatt gccccacggt tgagcaaaac cgacaaagcc
    27901 tgatgggcat ttttataacg acgcttatcc acaaagtcat cttgggtcag caagatttgt
    27961 gcagaaacga tttgacgcaa gagaagattg gtcgtatatt cttccaacaa aagtccctgc
    28021 cctaccgctg ctgaagcctg tttatcagca attttagtcg gacgcttttt aaatcctaag
    28081 gctccaaaac cagccgcaat ggcacctgaa gacaccaaaa tcaactcatg accagcctcg
    28141 tgcagcatag ccaactgctg ggtaatatcc tttaccttac tacgtgataa acttccatcc
    28201 tcatttgtca gagaagaagt acccacctta aagacaatcc gtttgtattt catagtctca
    28261 ctccgattat tcatatttat ccattataac atgaataaca ggaaatagaa aagagttgat
    28321 aggaaaaaga aggccctaaa accttctttt ctactactgt tctgattccg atttgtttac
    28381 actcgtttca gagctttgtg aagttgagga tgtcaaatct tgactagttg aaaaatttgt
    28441 aacactttca tgttgactcg tttctttatt ttcaagatcc tttttcacta cttctttgga
    28501 ttcttttatt tctgaatgac ttgtagtttt aattgtagca gatttctcga tcgaaacatc
    28561 cactaaccag gcaccacttg aatccacagt ataaccttct ggtgtcttac cgttcacaag
    28621 taattgacca ttttctttca agaagtacca tttatccttg tccttgatcc aacccacagc
    28681 tcgtgaaccg tctgtcttgt agaaatacca atcatttgcc tttttatgcc agccttgctt
    28741 catagctcct gaatcttcca gataataatg ttgaccagca acttctatct cacctgtctt
    28801 catgacacca gtagaatcca tatagtacca ggtttcttta tcttttaccc agcccatttt
    28861 catttcccct gatggagcaa agtaatacca ttgccccata tattgaagcc atcctgtctt
    28921 catagaacca tcaacatcca aataatacca ctgctcttta tcttgaatcc aaccaatttc
    28981 catctggttt tctttgttaa aataatacca gattccagcg attttcttcc aatttttagc
    29041 agaagcacca gagtctgtca aatagaacca atgattgttc cattttttcc attgattctg
    29101 taacaagata cccgtttggt taaagtaata ccattcacct tcgatttcat tccaaccgac
    29161 agcatactct cctgtagaat caggcgcttg ataccaccag ttcccatatg cactcttatg
    29221 ccaaccagct tgaaaacttg gaatcggctt gtaggatgtt gaaatattga caaaaccgtc
    29281 ttttcgaata tcaaaaactg ttgcatcata gtctttgctg gctgcgttga ttctctcaat
    29341 tcctcgttct ttgagccaat taacatactc actatcaaca ccatttttcc aaggtagact
    29401 atccgaagtt tgaacaatca aactcggact caaattttta atgaaatcct tggtatttga
    29461 tttgttggta tcatggtgat gattaaactt catcaaatca acttttccaa tgagaggacc
    29521 atacttgtct tctgctccat gaacattatc taaatcgccc ccaaggtaaa ttttcttgcc
    29581 attgactttc accacgctaa tcaaggaatt ggaattgtca tcccaaattt tctttaattc
    29641 acccgatgaa tcagtttcat tttcataatt atagagctga atatccatgt ccccaaactg
    29701 aaaatgagca tccccttgtg tgatattttg aataactgaa acaccttttt ctgcagcagt
    29761 ctgtaaaacc ttatcatagc catacagatt atcccataga cgttcagaat tagtaatacg
    29821 actatcacta tatttcttaa gatagactcg gtcaactgga taggtagaca gtaattcatc
    29881 aacatttcca atatgatcac tgtgggtatg ggtcaccaaa ataaaatcaa gtttttggac
    29941 acccaattcc ttcaaacgac gaaagacacg gtctgttaga acatgcttat aagacgtttc
    30001 aattccttct ctccatggat agcgagaatc acttccatct gggaaatcat aatcttctcc
    30061 tgtatccacc atggcaaaat gtccattgct ttcaagaata atcgcatcac tgccaccttc
    30121 ttgaacattg ataaagtgga ttttatttcc tgaactttct tgagcctgaa cattcccata
    30181 ccatgacaaa cctaaaaaag cgcctacaag tgctaaacta gttaatttct ttttcattct
    30241 tactttctca atctctccaa ggtttcctta aaaatctctg ggatatctgc tttaaattcc
    30301 aaggtcttac ctgttctcgg atgagtaaaa cctaaagtct tggcatgaag aaattgtcca
    30361 tgtcctttca aagtcttgcg aggaccatag acctcatcac cagcgactgg atggccgata
    30421 taagccatgt ggacacggat ttgatgagtg cgccctgtct ccagttgcaa ctctactaag
    30481 ctataatcgc caaagcgttc caagacgtga aaacgcgtca ctgcaggctt ccctttagca
    30541 gttacagcct gtttcttacg gtctttttca ctccggccaa tcggcgcttc aattacacca
    30601 cgatcattag gtagatttcc atgaacaatc gcccaatatt tgcggagaga ctttttatcc
    30661 ttgagttctt gggcaagtgc tagatgcgca tcatcgtttt tagcaatcat gagaagacct
    30721 gacgtatcct tatcaatacg gtgaacaatc cctggacgca gaaccccatt gatacccgac
    30781 aagtccttaa tatgatacat gagggcattt actagggttc cactggtatg accagcactc
    30841 gggtgcacaa ccattccctg aggtttgtta acgacagcca catcctcatc ttggtagact
    30901 atttctagcg gaagatcctc agccacatac tctaatacct ctggttctgg cacatggtaa
    30961 gtgacgacat caccctcttg gactgtgtat ttagctttct tgacttgacc attgaccaag
    31021 acctggcctg atttaatttg ttcattcgcg agactacgtg ataattctga caaatctgac
    31081 aaagccttat ccaaacgcag accaccagtt tcaattttaa tttccattta tttcctcttt
    31141 tagcattgca atcaataaaa taatcactcc aaccgtcaga tagctatctg ccacattgaa
    31201 aattgcaaag ttgataaagt caaggtggaa catatccaca acaaagccct gactgaccct
    31261 gtcaataaag tttccaagac cacccgcgat tattagagtc aaacccaaga ccatccagaa
    31321 tgagtcctcc atgtgtttat gtaaatacca aatggcacct atcacgacaa ccagagtaat
    31381 gacagcgaat aacaactgct gatcttgtaa gatagaaaag gctgcacctc gattttgcag
    31441 gtaggtcaag ctaacgaaat tggggatcca ggagcgcact tcacccagtg gaatctgctg
    31501 gacgatatag gatttgacca actgatccag cccaatcaaa agcagtacaa tgactgccac
    31561 tattgctctt tttttcatga tttcctcttt tgatcaaaat attcttgcat gacttctacg
    31621 aagagagtcc cagcttgact aagctccact tcttcacgtt taacatagac catgcggtta
    31681 tctaggttat ccttgagacg aataactgta atgccattaa cactgtcact atctaaaaat
    31741 ccagaacctg tcgcataggc gtccgtccgc tccaaaatac cattcaaggt ggcacggtct
    31801 gtcacattaa acatctgtga gctagcgctg gtatcaacaa agttctctga ataataaagg
    31861 tactcgtctt tctcttgagt gaaacgaacc gttggtaaat ccgctaaatc ctccatgact
    31921 aattcctctt tctgggctaa aggatgaccc tcacagagat aaatatgggt atggaaagga
    31981 atcaattcga tgacctccag acctaacttt tcaacccgtt gcataatccc ctttttattt
    32041 tgattgttga ggtagataat cccaatctca ctatgccctt gcgccacttc atctaatatt
    32101 tgaacagtag ttgattcaaa aatacggaag ttcttatagt caggatagcg ctctgaaaag
    32161 gccgtaatag ttggtggtaa gaagtcatag tgctggctag caacggaaaa ttcatctttt
    32221 tcttcttcag gattggcata ctgattttga aaaatatcaa atcctttaac caattcttgc
    32281 gctttttcat aaaattccat cccacgacgg gtcaagaaag tccctgagct ggtccgacgg
    32341 aaaatcttaa aacccaactc tttttccaaa tcccgaacag aaatagacag actcggctga
    32401 ctaacataca tcttttcagc agcttcacga aaagtaccac tattggcaat agccacaaca
    32461 tagcgtaatt gttgaatgtt catcttctac ccctaacttc tttatctttt cattatacca
    32521 tattttagaa gttttccaaa aaggaaaaaa gaacatccta ttcttcttaa ctatcttcac
    32581 tatctgcctt tctcacgcca atcttatttt caaaatcaat tcaaaaaaat aagtggctac
    32641 acaccacttc agtatcggaa agaaaaacgt tgactatttg tgaaaaaaga aaatgccgga
    32701 aaattccgac atttttttag taagctaact tcctgaaaat aggagtaacc aaacatccca
    32761 gaaggcagtg atattgatga gaatatgaac tagtattgga tagtagagat ttttagtcat
    32821 gcgatacaat agagctaaaa tcagacctcc actagcataa atgaaaaaag caagaggagt
    32881 taaagcaaaa ttgatatgaa tataaccgaa aataatagca gaaagcaaaa catctccgta
    32941 ccaaggtgag tttttgaaaa aggttgtcat aagcacacct cgataaatca attcctcagc
    33001 aataggggcg atgaagcaaa cgatgagcaa gaaataaggg aactcctgtc tccccatcat
    33061 ttctatcgtt tcattcaaag aaatctgatt tgaagacagg gatatgaaat acgaaaagag
    33121 gaagtcagac atatatgaaa tgatgtagcc cagtaaaagg tagatgaagt acctcagctg
    33181 ccacttgaaa tgaaaaattt tctttcctgc aaaaaccagt agatagatga ccgctaagaa
    33241 aagaactccg ctctccatca aaagcagaat ctgaaaaaat tcacgacttg ctggtagata
    33301 gggctttgcg aggtgattga aaactctcca aaaccaagtt gatttgtaaa aaattaagga
    33361 caatgctagt aaaatttgaa tagcccgttt tttcatatta aattctctgc tttctcccct
    33421 tgtttcttca tattactaat aaattttact taaaatctcg cagcacttcc tttgcaaaga
    33481 tgattgcctc ctccaatata tcctaaatca taggtgcctc tgggacaaaa tccatgaacc
    33541 ggcagagtta atactggttc tatttcccaa tcgtacctac cattaggatt tttaccatat
    33601 gaatctgctc caccttgtat cagctctaat tcttcatttg ttagatttat attttttgtc
    33661 attaaaatca tgatatattt cctccattat tcttcagatt agttgagtaa tctgatactc
    33721 atacctactt acaaaaaaac tattatatta agttggtttt ttaatttgtc tagttgcaac
    33781 attgacaaac atagtatagc atatctttgc gaaatatcct cttcaaatca tgaattgtca
    33841 tcaaaacatc ttaaactata aaatcaatta gtctcaagct ttctatcaat ttcttctcca
    33901 aaatatgcta taataatagc aaaagataaa gaaggaagac ctatgattaa actactagcc
    33961 ttggatatgg acggcaccct cctcaatgaa gccaaggaaa tcccacaagc tcacattact
    34021 gctattcaca aagccattga aaaaggtgtc aaactggttc tctgtacggg tcgccccctt
    34081 ttcggtgtcc tcccctacta caaaaaactg ggactcgacc tccagaatga gtatgtgatt
    34141 gttaacaacg gttgttcaac tcaccagact agcgactggg gcttggttga ctggcaagaa
    34201 cttagtccag ctgacatcga atacctctat gaccttgctg aaaagagtga tgttcagttg
    34261 acactttttg acgagtcaca ttattttgtt ctcggtggaa agcccaatca agttattgaa
    34321 aatgatgcta aactagtatt ttcagacctg actgaaattt ctcttgagga agcgactagt
    34381 ggaaagttcc ggatgttcca aggtatgttt ttaggaacaa aagaacaaac agacgatttt
    34441 gagcagcgtt ttgctgaaga gctttgccaa cgattcagtg gagttcgttc gcagcctgtc
    34501 atttatgaag caatgccact tggaacgaca aaggctactg ctctttcacg actagctgag
    34561 attttgaaga ttgattcctc agagattatg gccatgggcg atgctaataa cgatatcgaa
    34621 atgctccagt ttgcagggct tgggattgca atgggaaatg ccagcgatta tgtcaaatct
    34681 cttgcggatg ccgttacctc aagcaacgaa gaagacggcg ttgcgcgtgc tattgagaaa
    34741 tatattctat aaaaaagaaa agcaaataga cagaagttac tgctatttgc ttttttgcta
    34801 atattttaaa atacggacta atcaatagag aagaatagcg aatcataaaa atcgatttac
    34861 tagatgccat agtatcttat agttgctaat aagaagttag tctagcagaa tgaatctctc
    34921 atttctccca cactaatctg taatttgtct atacatttct ggtcttctat ctcgaaacaa
    34981 gccccagttt aggcgttcac ttgctccctt gtctaggtta taagtagcta acagaacagc
    35041 ttcttcttgt ctttcagctc gttctagaat agctcctgtt tcatccgtca taaaggagga
    35101 accgtagaag tcaagactgg aactctgtcc gccattttcc tcactaggag taacctcctc
    35161 taaaccataa cgattggctg cgatgactgg aacaatattc gctgctgcgt gcccttgcat
    35221 agtacgttgc cagtgaccac aactatctgt atccaaaatc ggctctgaac cgatggctgt
    35281 tggataaaag agcaattcag caccattcaa tgcaagacag cgcgctgttt cagggaacca
    35341 ttgatcccaa cagataccga taccaatctt agcatagcga gtattccaga ccttgaaacc
    35401 agtgttacca ggcgtgaaat agaatttttc ttgataataa tggtcatctg gtatgtgggt
    35461 ctttcgataa acgcccagca cttccccatc tgcatcaatg acggcaatag agttatacaa
    35521 gacattacca tctttttcat agaaactgat tggtaaaaca acttgtagtt ccttagcaat
    35581 caccttaaaa tgctgaatgg cagtattttc cgctacagat tgggcatact ggtagtagtc
    35641 atactgacgt tcctgacaga aatagggatg ttcaaacaac tcgggcaaga gaataatttg
    35701 ggctccttgc tcagcagcct gacgtactaa acgctctgcg gtttggatat ttgttgccac
    35761 atccttagcg cattgcatct gaatggttgc aactcttaca tttctcatct ttttctccta
    35821 ttctggaatt tgttgggtga tacagtggat attgccacca cctaagagaa tatctctggc
    35881 tggtattccg acaactttac ggtctgggaa acacttgctg aggatatcta aggccacttg
    35941 gtcgtttaca tcctcaaact gtggaaccaa gacagccttg ttggcgatat aaaagtttac
    36001 gtaggaagct gctagtcgtt cacctgcgta tcgcttttct tctccttctt catagatgta
    36061 gcctggcaaa tcttcttctg tcacaacttg tcgaactgca gggataggca atttatgaat
    36121 ggtgaagtga caaccttttg catctgtttc ctgttctaag agttcgagat ctgcttttga
    36181 catggcatac tggggatcat tttcgtcatc tgtccaagcc aaaacaagct cagcaggacc
    36241 aacaaaggca gcaacattat cgacgtgttc attggtttca tcctgataaa taccataagg
    36301 aagccaaata actttttcag caccaagact ttctaataat gtgttttcaa tctcctcttt
    36361 agtcaggtta ggattgcgac caggactaag caagcaactt tcagttacga gaatagttcc
    36421 ttgaccatcg ctatggattg cgcctccttc cagtacaaaa ggtttagcat catagacagg
    36481 cctttccaag gcctcagcaa aacgactggc tacttggtca tcctcttcat aatcttgata
    36541 aagaccatca taggtgcctc cccaagcatt aaaggcccaa tccacggcta atttcttacc
    36601 tttatcattg acgagaatgg ttgggccagt atcacgcgcc caggcatcat tggtgggaat
    36661 gtctaaataa acaaccttgt ctccaagata ggattgggct tcagatagat aggcctgctc
    36721 caccaaaaga tagactcttt ccccttctgc tatggtctcg atgatctgag taaatgctct
    36781 tttagcagcc tttccttgaa aaggccatga tcctggtcga gtcggccata tcatgagggt
    36841 accatgatgg ggttcgtact ctgctggcat gtgatagcct aatttttttg gactgtccat
    36901 catgataatc tccctttaaa gtcttgatag ccaaaagctt tgagtaagct acagtctccc
    36961 tgttcgtcca tgagatagag acttggcaat ccaataccat taaaggtatt atttttgaca
    37021 aaagaataaa tggccatgtc ttgaaaataa agtctgtctc cgatttggac tggattttca
    37081 aaactataat caccaatcac atcgcccgtc agacaggtat tagaagaaag tctgtaggta
    37141 tgggcttttt cctgtgactc aaagccattt ctcaaaggtg gacgataggg catctcaagt
    37201 acatcaggca tatggcaggt cgcagaggcg tctaaaacca agatttccat accgttttct
    37261 acaatatcta atacctcagt tgctaaataa cccgcattaa gcgcaatggc ttcaccaggc
    37321 tcgatataga tttcaagatt gtaagttttt cggatacgct tgatttctga aatcagcaaa
    37381 tccacatcgt aaccttctct tgtaatatga tgaccaccac ccatattgag ccattttacc
    37441 tcatgtaagt agggaccaaa ctgttcttct actgctttca aagttgtttg taaatcatct
    37501 gctccctgct cgcaaagggt atgaaaatga agtccgtcaa ccaaatctag caaatcactc
    37561 ggaatcttgt ctatagtaac tccaaagcga gaacctggtg cacaagggtc atagagcgcg
    37621 tgatctcctt gagttgaaca ctgagggttg aggcgcaaac caacactgac accagcctct
    37681 cgacaacgcg gaccgtgttt acgcaactgt ctctctgagt taaagacgat atggtccatt
    37741 atctctagca attcctccaa gtctgcatcc ttgaaagcag gcgcaaatac atggacttca
    37801 ccaggaaatt cttcccttgc caatttggcc tcatagagtc cactagctgt cgtacctgat
    37861 agatactggc taatcaaggg ataagttttg tagagggaat atgccttctg ggcaagcaag
    37921 accttgcaac cggcctcttc ttgtacatat tgtagaatgc ggcaattagc ttctaacttg
    37981 gccaagtcaa taacataggc tggtgttggt acttgttcta acttcattag tccaccattt
    38041 gtggattttc aaccacaacc catggcaaac catactcatt caaagcttcc atgaatggat
    38101 ctggatctaa ctcctcaagg ttatacactc cagcttgttt ccaagttccg ttcatgacta
    38161 attttgtccc aatcatggct ggaactcctg tcgtataaga aatagcttgc gaaccaacct
    38221 ctgcgtaaca ttcctgatgg tcgcagacat tgtagatata gatagtcttt ttgacaccgt
    38281 ctttgacacc tgtaaagata catccaatat tggtttttcc gactgtacgt ggcccaagac
    38341 tggcaggatc tggaagcaag gctttcaaaa attgaattgg aacaatttct tggccgttaa
    38401 agttaatggt atccgtacga aggagtccaa cattttcaag acatttcatg tgcgtcaagt
    38461 aagattgacc aaaagtcata aagaaacgaa tgcgtttgac acctggaatg ttcttggcca
    38521 atgattcgat ttcttcatgg tgaaggagat acatatcttt ttgtccaact tgagggaaat
    38581 catactcacg cttgatagac atagcttcga cttcgaccca tttcccatct tcccagtaag
    38641 aacctggcgc agaaacctca cggagattaa tttctggatt aaagttggtt gcaaatggat
    38701 aaccgtggtc accgccatta cagtctaaaa tgtcgatata atggatttca tcaaaatagt
    38761 gtttgagggc ataagctgaa aagacactag ttacacctgg gtcaaaacca gaaccaagaa
    38821 gagcagtcaa gcctgcttct ttgaatttct cttgataagc ccactgccat gagtagtcaa
    38881 agtaggctgt aaaaccaagt tccttacaac gtttttcgta gatagcacgc cactcagggt
    38941 cttctgtgtc ttctgcttcg tagttggctg tatcgatata gtgaacacct gttgccaaac
    39001 aagcatccat aatggttaaa tcttgataag gcagagctac attcaaaaca gcttctggtt
    39061 tgtagctttc aatcagggca atcacttctt caaccttgtc agcatcaagt gctgcagttt
    39121 caattttagt acttgttttg ccttctagct tcgctttcaa gtcatcgcat tttgacttgg
    39181 tacggctagc aatcataatc tctgtaaatg tttcgctatc ttgacaaatc tttgaaatag
    39241 caacttgggc aacgccccca caaccaataa ctagtaaacg actcattttt ttccttcctc
    39301 ttcttctaaa atgtcctcaa catacttggg caacataaag gctcccacgt gtaagtttgc
    39361 agtgtagtat tctgtgaaaa gctggcgttt tttccagcct tccttgtcaa aatctttgac
    39421 agggtggtat tttttcgatg caaatccaaa caaccaatag ccagctgggc tagttggaat
    39481 atgggcctga taaacccgac tgattggaaa ggcttgattg accttgcggt gcatgcttcg
    39541 gcaggccgac tcatcctcgt caaagaaggg actcccatgc tggtaaatca tgatgccgtc
    39601 ttccttcaga gctcgataac tattgccgta gaattccttg gtaaagagtc cttccgtatg
    39661 gccaaatgga tctgtcgcat cgttgatgat aatatcgtaa tcatcttcgc agtttcgcaa
    39721 aaagcgtagc ccattttggt agtaaatggt aacacgagga tcatctagcc ctgcagcaaa
    39781 gtctgggaaa tactcacgac agacctcgac caacatctca tccggttcca caatatcaat
    39841 ttgctccagt tcaggataga gggttaatac ttgggcaaca ccgccgtcac cacccccaat
    39901 aaccaatact ttctttggat ttgggtggac agccatggga acgtgaacgg tcatttcatt
    39961 gtagacgaaa tcatccgcat ctgagaacaa gacatggcca tttaaaatca gtattttccc
    40021 aaaagctggc gtatccaaga cttcgatatc ctgccattca ctttttccag cgtaaagttg
    40081 cttggctgtt ctcagagaca atttgacatc tggagtatga acttcagaaa accataaatc
    40141 catctatttc tccttatatt atatttagcg cagttagcta ctgccaaagc ccaagtggta
    40201 tacttggaat aagccactgt ggattagttc atttcctcca atcgcttgac gatttccgaa
    40261 aggctctaac cacagctttt gtttcaattg gtaatgagtt tgataacgca tagacgtttt
    40321 atctcgtcta aggtaacaag gcaaaaagtt cgtggataac caagtagact tgaaatacta
    40381 catctggagg tactcccatg ctttatgtgg gcattgatat cgctaaaaat aaacacgatg
    40441 ttacagcctt gaatgttcaa ggaaaaactg ttcttaaacc actcactttt tcaaataata
    40501 aagctggttt tgaactctta gatctgtctc ttcgacagct caaccaagac tgtctcatcg
    40561 ctcttgaaga tactggacat tatgccttta atctactgaa cttcctgcat gaacaaggat
    40621 ataaagttta cacctacaat cctttgctca tcaaggaatt tgccaaatca ttatcacttc
    40681 gcaagactaa aacggataag aaagacgctc atggcatcgc tcttaaactt ctttctgacc
    40741 ccaatcgtga acaatttcaa cacgataatc ggcaagtaga actaaaaata ctggctagac
    40801 atattcatcg tctcaagaaa aacagtctga ttggaaagta caatacactc gttgtcttga
    40861 tatcatcttt cctgagttgg ataaaatcgt tggaaagcat tcagaatata cctaccaact
    40921 cttgacacgc taccctaatc ctcagaaaag gattgaggca ggatttgata agctgataga
    40981 aattaagcga ttgaccgctt ctaattcaag ggctttttgc cgtgctctaa aatcatcgcc
    41041 aaatcatcaa attgaaataa gaaatagttt agataaaact attgactttt cttatagttt
    41101 gtctttctct taataacgtt gatatgattg acctctggat cttccgtccc ttggagggag
    41161 caaccacgtt ccttggcgaa ttggatatag tctacaattt ctcgtgtaat gcgttcacca
    41221 ggagccaaga taggaatccc tggaggatag cacatgacaa attctccaca gacctgtcca
    41281 acagactcat ctaaggtcaa actttttctc tctgaataga aggcttcttg tggagacagc
    41341 accaactcgg gctgaatata ttctccagct attaagtcct tcccatctcg tgaatagagt
    41401 ctcttaatat cagccagagc accaaccaag cgctcgatgt cttggatgcg gtcgccgatg
    41461 gaaatatagg ccaagatatt gccgatatca ccaaactcga tctgaatgtc gtattcgtct
    41521 cgcaagaggt cataaacctc gatacctgtt aagcctattc cctgagtgta aactgacaac
    41581 ttggttacat caaaatcaca gaccgacaca ccatctatta actcttttga gtaagcatag
    41641 taaccgccaa tggcattgat ttcatgacga gcgtactcgg atagctcaat gacttcctca
    41701 aacgactctt taccacgaag ggccaagttg cgacgtgaaa tatccaaact agccatcaat
    41761 aagtaagagg cagatgtaga ctgggtcagg tttataatct gacgaacgta ctcgggattc
    41821 atctgctccc cgattaaaag aagcgaactt tgtgtcaaac tcccaccaga cttatgcatg
    41881 gaaactgctg ccatatcagc tcctgcatcc atagcagaaa ttggaagttt accagtgaaa
    41941 tgcaaatgcg ctccatgggc ttcatctact aaaaccatca tgccagcttc atgagccatt
    42001 tctgtcaacc cctttaggtc tgaacagatg ccgtagtaag taggattgtt gattaggata
    42061 gccttagcat ctggatggtc ctttatggcc tgtgctactc ggtcattttc aagacctaaa
    42121 gcgataccaa tcttaggatc tacactcatc tcgatataga tgggaatggc accacataga
    42181 accaacgcat tgatagcaga tttatggaca tttcgtggca gaataatctt atctcctgcc
    42241 ttgcaggttg ccagaatcat agtctgcacc gatgaagttg ttccaccaat cattagaaag
    42301 gcatggctag ctccaaaagc atctgcagcc agctcctctg catcacgaat aatcgaaata
    42361 ggatggccta aattatccaa gggtttcatc gaattgacat caatgcctac acatttttct
    42421 cctaagagtt cgacaagttc tggatttccc cgtccacgct tgtgacctgg aacatcaaag
    42481 ggaacaatcc ttttcttgcg taacttcacc aaggcttcat aaattggggc ttggttttga
    42541 tctaactctt tcaaaacata ctcctttcaa tatttttagg accttatgaa aactaaggaa
    42601 ctaaaggttg actcatgaaa aaagagcagg ttttcacctg ctctgcaatc ttctgacgtg
    42661 tttattttgg tttctgttga gtatgtctgt tcccgatgtt tcctaactct ctagtagcaa
    42721 atattacgta ctttattgat cgtttcacaa gatgacgtgt gctttcacca cactaaaaat
    42781 ttatgaatta ggacaagtgt cctaacatca acttataaaa gtaggctttt aaccctatca
    42841 ataatgtggc ttttcaacca cgcttcggca ttcaaccctg cctgtccgta ggcatttttt
    42901 actcgggttt atatttgcta gaaaacatca tctcattttc taagctttat tactatatca
    42961 tgtttttaag aacttgtaaa gtattttttg ataaaaaaca aaaatatagt agattgaaac
    43021 tagaatagta cacttctgct tctaaaacat tattagaaat agattttact gtcctgatcg
    43081 atttgttctg ttcttgtttc attttactat atgttcgtaa atgaaagttc tataatgaag
    43141 ttagccaccc agccctccta aaaattagat ggttcaacta gctgatagct cttgtaggca
    43201 taagaaaaca cctctgtgtt atacttgttg ttcaaccaca aacacaagaa aggcacagag
    43261 atgcaagaca attatactac aaaagccaaa catttgacaa tcgatagccg tcgcttaatc
    43321 gaaagatgga aaaaagaagg gaaatcaaat agagaaattg cctctctgct tggaaaagtt
    43381 cctcaaacta tccacactga aatcaagcgt gggacagtcc gacaatgtct tggaaaaggg
    43441 cgcttcaaag aggtttattc tgccgactat gctcaacagt cttatgaaaa caatcgcaag
    43501 cgctcggtca ggaaatcaag cgtgaccaag gaactaaagg aaaagattct ccactatcat
    43561 aaccaaaaat tttcgcctga aatgatggtg atggttaaag gggttaacgt gggaatttca
    43621 accatttact attggattca tcatggaaaa ttggggttaa gcaaacagga tctgctttac
    43681 cctagaaaag gaaaagcgct taagaaacag gctagcacca actttaaatc tgctggtcaa
    43741 tccatcgaac agcggtctga agctatcaat cttcgcttgg agaatgggca ttatgagatt
    43801 gatacggttc tacttacgag agcgaaaaac tactgtttgc ttgtcttgac ggatcgaaag
    43861 agtagacatc agatcatccg attgattcca aataaaagtg ctgatgtggt caatcaggct
    43921 ctaaaactca tcttaaaaca acacaagatt ctttccatca cggcagataa tggaacggaa
    43981 ttcaatcgct tgtttgatgt attttctgag gagcacatct attatgcgca cccctatgcc
    44041 tcttgggaaa ggggaactaa tgagaatcac aacaggctca ttcgtagatg gttacctaag
    44101 ggaatcaaga aaatgactcc caaagaagtc gcattcatcg aaaagtggat taacaactat
    44161 cctaaaaaat gcttggacta caagtcaccc agagaagact tctggatggc taacttgaac
    44221 ttgaaattta gcgtcagaaa caaatcacga aattgattca aattttgcag gtcgtttaaa
    44281 tatcctgcgt gcgggtgttc ttgatgctaa cgatggaatt atttccattg ctggtgtggt
    44341 tatcggagtt gccagtgcca cgagcaatat ctggattatc tttttatcag gttttgcggc
    44401 tatcttagct ggtgcctttt caatggctgg tggagaatat gtatccgttt caactccaaa
    44461 agataccgag gaagctgccg tttcgcgaga aaaactcttg ctagaccaag atagggaact
    44521 agccaaaaaa tccctctatg cttcttatat ccaaaatgga gaattcaaaa cttctgccca
    44581 actcttgact aataagatct ttcttaaaaa tccactcaag gctctggtag aggaaaaata
    44641 tgggattgag tatgaagaat ttaccaatcc ttggcacgct gccatttcta gcttcgttgc
    44701 ctttttcctt agaagtttgc ctccaatgct gtcagtgacc atttttccaa gtgattaccg
    44761 catccctgct accgtcctta ttgtcggtgt ggcccttctt ctcactggtt acacaagtgc
    44821 tagacttgga aaagccccaa ccaaaacagc tatgattcgg aaccttgcta ttggtctctt
    44881 gaccatggga gttaccttcc tgctcgaaca acttttcagc atttagaata caagaaatac
    44941 ctcgattttg aagtcgaggt atctttttta catttgcaca atcttgcgat aacttcttga
    45001 agtaatcata aaaatcagca cataggcgat gaggaagata gcgcagatag acaaggtcac
    45061 aatcaacatc atagtcgtat ccagtacacc aatcactttt aaaatcaggc taagcatatg
    45121 gtaggcaaag gctagatgta ggaaggcaaa gagcaaagga aggaagaaaa cagttaaaac
    45181 ctgtttgttg atggtttgct tgatttgctt ttggtccaaa ccgactttct gcaagataat
    45241 aaagcgttca cggtcttcgt agccttcaga aatttgtttg tagtagatga ccagaacagt
    45301 tccgaccata aagataatgg ataggaaaat accgataaag aagacaccac caaagagggc
    45361 actcatctga gcactagcat ctgctagagt gctaccataa acatagctac cttctgtgtc
    45421 taattgagca ttaaattgat tgaggtagtt ttcatactcc tcagcgacct tgagttgttc
    45481 ttcttcgctg gcatctacat tcataccacc gtaaaactga ttatagatag ccgaatctgg
    45541 gaattgatcc aaaaaggctt gtaaatcagg aacaacaagg taattgtaat cagtagtcaa
    45601 gatattaaac ttatttggaa catggttgac aatgaaatct ttattaaatt cttctttgac
    45661 agaaaattga tgatcattta gagttagagc tttctgtcct ttcagtccgt catttttggc
    45721 aaagagaccg acctcatttc ctgatagaga cagtttttga ccagtcatat tttcataatc
    45781 tttttggtca aataccatga aaactgtttt tggttggaca cggttttgtc ctttttcaaa
    45841 aatagttaac ttggttcctt cttgatttgc aataccaaag ttactgtaac gaagcacttc
    45901 tttctcttta atcttataac ctttgtcact tgcaaactgg ctcaagagtt tgtccaaatc
    45961 ttctttttca acattttgcc ctgaaacccc aaaatcatga ggatttagaa cttttttaaa
    46021 ggattctgcg gaattgaaaa tgcttgtcgc tgctgacatg gttaccaaaa ccattgttga
    46081 caaaatagcg atagttgcta gtccaactgc atttttcttc atacggaaaa tcaagttaga
    46141 aacagatatg agattattag gttggtaata gtatttctta tttttcttta agatttgaag
    46201 gaaaacggta atccctgcat tgaacaaaag ataagtacca aagataacca gtaaaacagc
    46261 tatgaagaag gttgttaagg ctgtaagagg atcttttacc gtaagggcaa gataatatcc
    46321 aatccctaaa cttatggaac caagaatagt ttggagagga aggaagcgac cttttttctc
    46381 tccgctagct ttctcacgtg agagctggag ggcattcata cgggcgattc gaagggcatt
    46441 caggaacatg aggcctagga aaatcaaacc gaagacaaga agtactgtaa tgacaacttt
    46501 catctggaag gtagcgacca gttccacctt caatttcatc agtttgagta ggaaagcgaa
    46561 aatcaacttg tcaaacaagg ctccaatacc gatacccgct ccaacagtta gaatcccaaa
    46621 taccactaac tccttaaagg tcatactgat aagatgacgc ttctccaagc ccaacatgcc
    46681 ataaattcct agttccttgg aacggttctt catgacaaaa ctattggcat agagaacgat
    46741 aatggctgac gcaagggtga cgacaaacat accaaatcca agtgtagcct gaatggttgt
    46801 tcctccacgg atttccgcaa tcttaggatt gaaggttaga gagtaaaaga gataggtgac
    46861 agtgactgcc aagagaacag ccagcgcaaa aggatagtag agtttgcggt ttttaatcaa
    46921 gttcgatacc gctaacttat tggttaatcg aaacatacta attcacctcg cttgccatga
    46981 cagtcaaggt atcagagatt tcttggaaca tctgacgttc tgtcttctct ccacggtaga
    47041 tttgattgta aagaatgccg tctttgataa agagtacacg tttagctctg ctagctgctg
    47101 ctgttgagtg ggttaccatg agaatggttt ggccgcgctc attgatttca tcaaagacat
    47161 caagtaaggc tgcagatgac ttggagtcaa gagctcctgt cggttcgtcc gcaaggagaa
    47221 tttcaggttc tgtgatgatg gcgcgggcta ctgctacacg ctgtttctga ccaccagaaa
    47281 tctcgtaagg gtacttctct tgtaattggt ttattcccag attctcagct gtcaccacca
    47341 atttcttcat catctctgta ataggtcttc ttgacaagac aagcggaagc aagatattgt
    47401 cctttacaga tagagtatct agcaagttaa agtcttggaa gacaaagccc aacttttcac
    47461 gacggaagct agaggcctgt gaatttttaa tggttgcggt gtcagttcca ttcaagtaaa
    47521 cctgaccacg acttggttta tccagcatag ctagaatatt gagaagagtt gatttaccag
    47581 aaccagactc acccatgatg gcaacgtagt cacccttttc gacggtaaag tgaatatcct
    47641 tgagggcttc tacttggttg ccctgaaaac gtgttttata aattttttga acgtgtttta
    47701 catctaaaag tgtcatgaga atctcctttc ttaatatccc atcaaatgaa atgtagcttg
    47761 catcatagtg catcccagct gaacgcgctc aatttctttg tgacttggtt ttctagtttt
    47821 cttatgtaag atttgtttca tgatgttact cctttgttct ttatgagtct agtttacatc
    47881 aaaaaaagac cgcttgccat aacctaacct tacaaaagag actttaatct tacatttttg
    47941 taagattggg tgaaatataa gcaaaattac ttagaaaatg atttgatttc ctatttttaa
    48001 agttgtaaaa tataagtgtg aaaaggctta ctttgaaaag gaggaagcgg gaactaacat
    48061 tttagtaagg ttacctagat aacataccat tcaatttaac acttgcaagt cgttcaacct
    48121 ataaggaaag aaggtgcgct ttaatgaaac acaaagaaca tatactcatc ggacttctct
    48181 atctgctctc tccattcatc ggtcagctct tagttgaaca cacacacttt atcggtacag
    48241 aatttaccgg cactgcttat gttatttgct ggttatcagt tgttatcagc atccaccatt
    48301 tttccaaaaa tgtattgtct caacagcaaa aatagattaa aaaactgcgt ttaaattact
    48361 gcttttatag taaaatgaaa taagaacagg acaaatcgat caggacagtc aaatcgattt
    48421 ctaacaattt tttagaagta taggtgtcct attctagttc caatccacta tagtttgtgg
    48481 atgaagctgg cgaaatatta ttaatactat tcaaaaatca aagtgcaaac tatatcaact
    48541 tcacattgcc gactaaatta cagcctgcgg ctggcttcct agtttgcact ttgattttta
    48601 ttgagtataa aagtaaaaaa gctttaaatc aagcagttta gactattgat ttaaagcttt
    48661 ttgcgtattc caaatctttt gtaaaacctc accaaaacca gccttcatta tcgtcaatgg
    48721 cttgagcttc tttgcgttta cgtgggcctg ttccgtacat ttctgcttcg atttcttcct
    48781 tggttggcat gatagaagct aggatgatat agataatcac cccaagtcca aagtttgcga
    48841 ctgtaaaaat ggcaaacaga aaacgtacaa gggtaacatc aaagttccac ttgtctgaca
    48901 gacctgctaa aactcctgaa atcatgcgat ttcgtctcat tttataaaac ttactgttca
    48961 tgatatttcc tctttctgct aggttagact agtatatcac ggataggctc ggctttcatc
    49021 agtcctcagg ctgatttact aatagcaact ttcctcgaca aagtccacag cgatagcgtt
    49081 gggtatcaat cctacgctta cgctgataag tttgctggca ggattggcaa cgatagagct
    49141 tgattggctt ggagttacta ttgggcaagg atggtacaaa ccgtaatcca tccactgctt
    49201 tcaacagttc cttaaaatcc cgatccttgt gttgatagcc tttcccttga aaatagaggt
    49261 gataatgaca gagttcatgt cggacaattt tcctaaaaac gtccaacccc agttcctgat
    49321 aaaccttggg attaaaatcc aaatgcccat ctttggggaa aaatcgccca cctgtcgaac
    49381 gtagacgcct attccactgg acatgatgga taaaaggtct gccgaagtct tctagtgaaa
    49441 cctgcttgac gtaatcagtc agtttcattt ggagctagga gagacagatt aactttttca
    49501 cgttcagtat caattttctt aacccaaacg ctcaccaaat ctccaactgc caccacttga
    49561 ctagggtgtt tgataaactt gcgactcata tgggaaatat ggatgagacc gtcctcatga
    49621 attccgatat caacaaaagc accgaaatca acaacattac gcaccactcc ttctagcttt
    49681 tgtccaacca ctaagtcctt gatatctagg acatcttgac ggagaactgg agcatcaaag
    49741 gaatcacgga aatctcgacc tggtttgaga agatctgcaa tgatatcttt aagagtttct
    49801 ggaccaaggt ctaactcttg cgccatttcc ttgactgaaa gcgacttgag tttactttgg
    49861 gcctcttcat tcagatcctt aatgtccaag catttgaaga gttccttaac ggcagcgtaa
    49921 ttttctgggt gaactcctgt attatcaagg atattgctac tttcagggat acggaggaaa
    49981 ccagcagcct gttcaaaagc cttggccccc aaacgaggaa ctttcttgat ttgggagcgt
    50041 gaagtgattt ttccctcttc ctcacggtat ttgacgatat tttcagaaat ggttttattg
    50101 agtccagcta cgtgtgaaag aagagctggg ctagctgtat tgacattgac accaacttgg
    50161 ttaaccactg tatcgacaac aaagtccaga ctctcagata gtttcttctg actgacatcg
    50221 tgttggtatt gaccgacacc aattgactta ggatcgattt tgaccaattc cgcaagagga
    50281 tcttgcaaac gacgggcgat agaaatggca gagcgttttt caacggtcaa gtctggaaac
    50341 tcctgacgag caagttcgct ggcagaatag acagaagcac cactttcatt aacgataaca
    50401 tagctgactt cagggaaatc tttcagaact tccgctacaa aagcttcact ttcacgactg
    50461 gccgttccat ttccaatggc aataatctct acaccgtatt gaccaattaa atctgctaaa
    50521 tctttcttgg cttcttcgat ttgacgagct gatgctggtt taacaggata aataacctga
    50581 gttgtcagca tttttcctgt tgcatccacg acagctagct tggcacctgt acgaaaggct
    50641 gggtcaaacc caagaaccac gcgccctttc agtggagcaa ccaagaggag attgcgcaga
    50701 ttgtcagaaa aaagttggat agctccctct tcagctttct cagttaattc tgtccgaata
    50761 cgacgctcaa tagcaggcaa gacctttttc ttaacggatt gctgaacaac ttcatcaata
    50821 taagcatttt tcaccttgaa acgagtagca aagaaggcaa gaatacggtc cgtcgcatgt
    50881 tcaaaaccga tcttcaagac accaagtttc tccccacgat tgagagccaa ggtacgatag
    50941 ccttgcatag ttccaactgt ctctgaaaaa tcataataaa tctgaaaaac ctgcttttca
    51001 tcaagacttt catccttggc ttgagaagtg agtttagagt gtctcagcac ttcctgataa
    51061 gtcatagaac gcaaggtcac atcttccgat aaggcttcga ccaaaatatc aactgcaccg
    51121 gtcaaggctt ccttgccagt cgcaaatcct tcacagacga acttttcagc ttctttctct
    51181 aagtcaacta tattctgcaa aatcaaacga gcaagaggaa agagtccagc ttcacgggca
    51241 atggttgcct tggtacgacg cttttcctta taaggaagat agagttcttc aacgtctgct
    51301 aatttttcgg caactaagat agcttcttcc aattccttgg tcaacttacc ttgttcttga
    51361 atcttagcta agacagcttc cttacggtca ttgagatttg tcagactttt atccaaatca
    51421 ataatagcct taatcgccac ctcatccaga ctaccagtca tgtccttgcg ataacgcgcg
    51481 ataaagggaa tagtcgcccc ttcagctgtc aaacttagaa cggtatcaat ttgctttaac
    51541 gtcactccca aatcctgaga gattttttca tattttttat ccataaatct attataccac
    51601 aagctaaacg tttcaaatta actcgtagaa catttaaaaa atatgtagga aatagattta
    51661 tatgctacag cgcaataact tgcacttaaa gagcattgcc accttttttt aaccaagcca
    51721 tgatatcaaa agtatttaat ggatcagaca taatagccag ttctggaaga tgttcctgac
    51781 ctggaataac acattgactt ttcaaatttt tatatggacg attgactaaa attaatttat
    51841 tagaataagg aagattatcc atcttattta aaatttcttc actagctgaa tctttattat
    51901 caaatttaaa ataaagatta ttccaattta tgcgtttttt tcttttttcc cacttagttc
//
LOCUS       DAVKMU010000009        48874 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKMU010000009 DAVKMU010000000
VERSION     DAVKMU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.009"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(35..397)
                     /locus_tag="VW343_000604"
     CDS             complement(35..397)
                     /locus_tag="VW343_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755205.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNIEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(688..2568)
                     /locus_tag="VW343_000605"
     CDS             complement(688..2568)
                     /locus_tag="VW343_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9755206.1"
                     /translation="MVRFIGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2562..3431)
                     /locus_tag="VW343_000606"
     CDS             complement(2562..3431)
                     /locus_tag="VW343_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9755207.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3515..6160)
                     /locus_tag="VW343_000607"
     CDS             complement(3515..6160)
                     /locus_tag="VW343_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9755208.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(6251..7531)
                     /locus_tag="VW343_000608"
     CDS             complement(6251..7531)
                     /locus_tag="VW343_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9755209.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKNFLLDQLVACDGGTGLMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7702..9786
                     /locus_tag="VW343_000609"
     CDS             7702..9786
                     /locus_tag="VW343_000609"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9755210.1"
                     /translation="MKPLLETIDTRFGTASKHAFSWGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKASAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(9826..11505)
                     /locus_tag="VW343_000610"
     CDS             complement(9826..11505)
                     /locus_tag="VW343_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9755211.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGSNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12260..13489
                     /gene="arcA"
                     /locus_tag="VW343_000611"
     CDS             12260..13489
                     /gene="arcA"
                     /locus_tag="VW343_000611"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9755212.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13547..14563
                     /gene="argF"
                     /locus_tag="VW343_000612"
     CDS             13547..14563
                     /gene="argF"
                     /locus_tag="VW343_000612"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9755213.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14728..15675
                     /gene="arcC"
                     /locus_tag="VW343_000613"
     CDS             14728..15675
                     /gene="arcC"
                     /locus_tag="VW343_000613"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9755214.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNSGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15886..17397
                     /locus_tag="VW343_000614"
     CDS             15886..17397
                     /locus_tag="VW343_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9755215.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17419..18750
                     /locus_tag="VW343_000615"
     CDS             17419..18750
                     /locus_tag="VW343_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9755216.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEENLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            18845..18972
                     /locus_tag="VW343_000616"
                     /pseudo
     CDS             18845..18972
                     /locus_tag="VW343_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(19137..19427)
                     /locus_tag="VW343_000617"
     CDS             complement(19137..19427)
                     /locus_tag="VW343_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755217.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(19415..19666)
                     /locus_tag="VW343_000618"
     CDS             complement(19415..19666)
                     /locus_tag="VW343_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9755218.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFDGMGDQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            19870..19947
                     /locus_tag="VW343_000619"
                     /pseudo
     CDS             19870..19947
                     /locus_tag="VW343_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20065..20949)
                     /locus_tag="VW343_000620"
     CDS             complement(20065..20949)
                     /locus_tag="VW343_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9755219.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(21094..22245)
                     /locus_tag="VW343_000621"
     CDS             complement(21094..22245)
                     /locus_tag="VW343_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9755220.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(22391..24157)
                     /locus_tag="VW343_000622"
     CDS             complement(22391..24157)
                     /locus_tag="VW343_000622"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9755221.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(24214..27330)
                     /locus_tag="VW343_000623"
     CDS             complement(24214..27330)
                     /locus_tag="VW343_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9755222.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAISEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            complement(27340..29634)
                     /locus_tag="VW343_000624"
     CDS             complement(27340..29634)
                     /locus_tag="VW343_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9755223.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRV
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(29693..30493)
                     /locus_tag="VW343_000625"
     CDS             complement(29693..30493)
                     /locus_tag="VW343_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9755224.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(30490..31263)
                     /locus_tag="VW343_000626"
     CDS             complement(30490..31263)
                     /locus_tag="VW343_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9755225.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(31288..31758)
                     /locus_tag="VW343_000627"
     CDS             complement(31288..31758)
                     /locus_tag="VW343_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9755226.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(31749..32180)
                     /locus_tag="VW343_000628"
     CDS             complement(31749..32180)
                     /locus_tag="VW343_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9755227.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(32173..32613)
                     /locus_tag="VW343_000629"
     CDS             complement(32173..32613)
                     /locus_tag="VW343_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9755228.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(32625..33263)
                     /locus_tag="VW343_000630"
     CDS             complement(32625..33263)
                     /locus_tag="VW343_000630"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9755229.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            complement(33378..34781)
                     /locus_tag="VW343_000631"
     CDS             complement(33378..34781)
                     /locus_tag="VW343_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9755230.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELNSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            34957..35730
                     /locus_tag="VW343_000632"
     CDS             34957..35730
                     /locus_tag="VW343_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9755231.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(36901..38406)
                     /gene="adcA"
                     /locus_tag="VW343_000633"
     CDS             complement(36901..38406)
                     /gene="adcA"
                     /locus_tag="VW343_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9755232.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(38416..39222)
                     /locus_tag="VW343_000634"
     CDS             complement(38416..39222)
                     /locus_tag="VW343_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9755233.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(39215..39919)
                     /locus_tag="VW343_000635"
     CDS             complement(39215..39919)
                     /locus_tag="VW343_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9755234.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(39919..40359)
                     /gene="adcR"
                     /locus_tag="VW343_000636"
     CDS             complement(39919..40359)
                     /gene="adcR"
                     /locus_tag="VW343_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9755235.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(40514..41782)
                     /gene="dltD"
                     /locus_tag="VW343_000637"
     CDS             complement(40514..41782)
                     /gene="dltD"
                     /locus_tag="VW343_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9755236.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(41775..42014)
                     /gene="dltC"
                     /locus_tag="VW343_000638"
     CDS             complement(41775..42014)
                     /gene="dltC"
                     /locus_tag="VW343_000638"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9755237.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(42028..43272)
                     /gene="dltB"
                     /locus_tag="VW343_000639"
     CDS             complement(42028..43272)
                     /gene="dltB"
                     /locus_tag="VW343_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9755238.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(43269..44819)
                     /gene="dltA"
                     /locus_tag="VW343_000640"
     CDS             complement(43269..44819)
                     /gene="dltA"
                     /locus_tag="VW343_000640"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9755239.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(44840..44971)
                     /locus_tag="VW343_000641"
     CDS             complement(44840..44971)
                     /locus_tag="VW343_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9755240.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(45285..46463)
                     /locus_tag="VW343_000642"
     CDS             complement(45285..46463)
                     /locus_tag="VW343_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9755241.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            complement(46915..48191)
                     /gene="ltrA"
                     /locus_tag="VW343_000643"
                     /pseudo
     CDS             complement(46915..48191)
                     /gene="ltrA"
                     /locus_tag="VW343_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT        14487 a        10692 c         8819 g        14876 t
ORIGIN      
        1 tgaaatacta agcctacaca gactgatttc aattttacca ttcttcatag tcaaaaccat
       61 cataatctgg atattgttct ggcgacgttc caaccgaacg tacgagataa gcttcttcgt
      121 cctctgtctc gatttctctc agcacttggc attcaaaacg ccattcatca ccgaagtcga
      181 aaataaactt aaatttttgt ttgacactta ggctatccag atagacattt tctgtgtaac
      241 gttcttcaac atcgtcacta acaaagctaa gaaagtaaga atctgcatga ctccactcaa
      301 tattatccat gaaaaatgcg tgagcatgat catttgcaaa atcaaaactc cataaaatca
      361 catctgctag ttccgctaac gtttttgttt ttggaatcta tatatgtcgg tagcaacctt
      421 ttttaaattt gacggaaatg atccaagcct ctacagatgc tggcttttca gcttctattt
      481 cttgttttac taattttaac tgtagcattt gaaaccagat aattcttctg tttcacgcga
      541 aacaccctgc gccaagcaag gtgttcttgt ttttatactc aatgaaaatc aaagagcaaa
      601 ctaggaagct agctgcaggc tgctcaaaac actgttttga ggttgtagat ggaagctgac
      661 gtggtttgaa gagattttcg aagagtatta agtcgtataa atcgtcgaag ctgtcgcaat
      721 ggtatgccac tggttagctg tagttgctgc gaaatccttg tctgcgtaga agtcagtaaa
      781 tggtaggatt tcaacttcca gctcgtcgat gcggtcaagc tggccagcca gataaacctc
      841 gatacggctt tctgctcgtt tgatgcgttg caagagtccg cccatacgga tgtcaactgt
      901 atccaaacca aagaccttgt tttctttcag ccattggtga ctaaagaggg catggaagtc
      961 ttcaatttgg cttctaagtt ctggtaattc ttgtctggcg atttgttgta aactttcttt
     1021 atcatccgct tggtaggcct gacgaatgcg tcgtcccaca tctactttgc tacttaaaat
     1081 agcattcaac tgggcctgag tttcaaagag ataagcatag tttccagctt tttctttaat
     1141 gttagcaagc gtctcagcag cctgagcgaa gtgcggtttg tcctgttcag gtgtcatgtg
     1201 tcggtcaagt atcggacaga gaacatcctg ataaaagaca tatcggttgg gattgatgcc
     1261 actgagattg cctggtaggt ctggtaagag gttggcaagg tcaatctgca taaaatcctc
     1321 aaccgttaga ccagtattgg tcttgaagtg cgcagacaaa ctatctaggt catggcgata
     1381 gctgagttct gcccagattt gcaagcttgg taggatagag aactgggcag tttcaccccc
     1441 attgtctccc caacccgtta cgatgacttc tttaatctga ttggcacggc aggctttatt
     1501 agcctcgata gccactagac ggctaaaatg gttgtgaggt gtaaagccaa tccacttcca
     1561 agctccccct gcaaatgcaa ggtcatggct aatcttgtga tgattgcgga aattacggtt
     1621 gtatttttcc tcgctatcct gataataatc ccagtaaacc agagtcacac ggtctttgag
     1681 acggtctagg tagacacgag tttcctctgg aatttccaca tcacggtcgt actggccatc
     1741 cgctgacatg agtttgaaga acatatcact ccacatctgg cagtggaaac catatttgtc
     1801 agcaatatcc agcacgcgct ccaagtgttg gcacatgagg agactacgat ccacaacacc
     1861 gttcaaaatc aggtagcgtc ccaaaccaac caagtgggct tcgtccatcc cgatattgac
     1921 cttgcgagtc ttcagtttag acaacgtggc aaacatgcca tcaatcaggt cataaacctt
     1981 ttcttcgcca atgagaagaa tgtcctctac atcacggagc tcctgcactt ccttgacacc
     2041 ccatttgaca aaggccgaca agtgggccaa ggtctggatg catggtacaa aggtcatgtc
     2101 aaactgttgg gcataggctt cgatttcctg caactcctct gctgaataag ctccacggaa
     2161 atagccaaag taaggctgtc cttcaatctg gtaagtgtct tccatgtaaa gctcaaaggt
     2221 tgagtagccc atgagagcca agacctcaat catctgcttg gcagaagcca cattcagcac
     2281 cgcatttcga gaacagtcaa ccatgtaagc caaatcttcg taagctgctt gttcctcaat
     2341 ctctacttta tcagcttctg ctagaactgt taccaacaag gacaaggcac gataaagttg
     2401 gtgaggtttg cgataggtca attgatagtg accttcctca ccctggatag agatagatgc
     2461 ttggtcagac tgagtgacag ccacttccac atctggtaga gaaatgtgac cttttaaaac
     2521 ctcaatagct tgcgtttgtt tgggactaag tcctataaat cttaccactg cttttcctcc
     2581 tgtagccagt tgacaagagc accgtagaga ttggcatctg cgtgataggt gcaggcctgg
     2641 ataactggtg cgaccgtgta ttcttcgtag gcatcgacaa agtcttcaac agccttctta
     2701 acaccttgga taaaatctgg attttgactg atagagccac ccagactgat gacatctgga
     2761 tcgatgagat actggatatt gagcaagcct tgcgtcaaat tgcggttcat gcgctcaatg
     2821 gcttcttgac aaagagcatt accagctgcg gcctcttggt aaatcttgcg accgtcccaa
     2881 tcagtatgac cagatttttc aatcacgtat cgtaccatat tcccagttga tgctagttgc
     2941 gaccagttat taagtttttc agcaggggca agggttgtca tgtagccaaa ttctccaccc
     3001 agaccgtggc gacctcgatg aagtctacca ttgataatca tggctccgcc aatccctgtc
     3061 ccaatcacga cacaggctgc attttcaagc tctggatgag ctagtagttc actgagtcca
     3121 acgcagttgg catcattttc taaatggaca ggtagctgat aagagctaag cgcctcatac
     3181 caagaaaagc catggatgta gggcaccgca ctgaagccat caatcacacc tgtctcttga
     3241 ttgactgcac ctggaacact catagcaatc ccactgtaat cctgttctga caagcgttga
     3301 tctagccacg ctagtaaatc ctccaagttt tcaggcgttg aaatacttgt cttatccagt
     3361 attttcccat caggagtcag actggcaaac ttaatcccag tccctccgat atcaatcgtt
     3421 gcaatggtca ttgtttttac cataaaaagg ggcgaatcag cagttagttc ttactttttc
     3481 gcccctcctt tcttttgatg tttacttttt gaaattaaat ttcttctttt ttgatgaatt
     3541 ctgtacgaat ctcttgtgga gccaatagtc ctgaatgaac tggatatggt cgctcaagta
     3601 agtcaagaat ggtttgttga tgttctgata tgcgcacatt ttcttgactc atattatagt
     3661 aacggaggac atagccttct tcattttccg ccactttaaa ggctgttgga caaacttgcg
     3721 gtatgctgag aacagaatgg ctcaagaggc taccagtcgc aaccacgctt ccttcctgtc
     3781 tagcaagctg aaggctggta aacggtgtct gcaaggcttt ggcacgacga taggctgaga
     3841 agcgttcttg ggcttggtgg cattcaatcg caaattcgac ttcaaactca cgcaagcatt
     3901 gtgcttctgg cgttgggaag tagccccagt cacctagctc acctgatgca cgcaaaatgg
     3961 tcacggcaat ggtgttatcc ccaaggattt cgtattcatt caatcccttg ttggatacag
     4021 tcaccccttt ttcatcgtca tacagactga caaaagcttg ttggtgttga ggattttcag
     4081 gattttccca tgaagcagct ggtttgtttg gtcgtgtcac cacctcatag atactttcag
     4141 aatcattgct tggacgcgtg ttatgagtct tgaccaagag acggatacgg tgatccttgg
     4201 cagtgttagt aaagcgagtc ttgaagcgga tttgtggatt gtcaacaaag acaatcaact
     4261 cagtttccag aggaatgttt gtcaattctt ctgaccgtcc agcctcacgc ttcataaact
     4321 cgatgatacc ttgttgctct tcttctagct tttcatccgc actgacaggc acggtcaatt
     4381 catgtttgag caagatttta gcatagcgat ctgtgttttc caagacctcg tggcccttaa
     4441 gctctgcaaa gattggctct gttccttttg gttggaaata gatatactcg tttccgatgt
     4501 caccccggtc ttcaaagcgg ataaagtctt cataggcttc gtgagttgtc ttgtcataga
     4561 ctgtgatgtt gtcatccaca ctcaccgtta cgaatggtgt atcaatcact ccgttttggt
     4621 aaataccctc acggtgttct tgttttcctt ccagcaattg gaaggttgtc caagaaagcg
     4681 gcgctaggtg aactggaatg gtcacgcgca cttgtcgagc aatacgagct tggcggaact
     4741 tatcttttgg taaatcgtac tcaaaattag ctccgaggtc ttcgattgta gcctctacag
     4801 gacgaccatc caagtcctcc acacggtaac ttggcaagat aagagcagcc atctttttgt
     4861 agccttctgt tgggtgcaat tccttgaaat cacaagtcgc cacatcaatc actgtgctga
     4921 cagtatcgac cttatcatgc aagcctgtgt taatgacagt aaagagatag tcactttgag
     4981 ccttatccgt agcaatttta cccttccact cgttgagcaa gttactttta acaaagtttc
     5041 ctacttggtt gaccttggca aaacgcgttt ccatctcgcg gtgaacttcg tccacgctac
     5101 agccacagat actatcatgt ggcgcgttct gcaaaagtgt tttccaagca taggtcaact
     5161 ggtccttgtg gttgtgtcca ccagtgataa tagtcaaggg ttctaccact tgctctagga
     5221 ggttgctatt ttcttggaag gcttgtttca ggtaaatgcg ggatgaagaa gtgttggcaa
     5281 gtgtgtacca gccatctgtt tcctgactgg tcaactcgcc tgtaacagtt gataagtgtt
     5341 caggaagcgc accttctaca gcttgaacat attcatcaaa agaactatga acaaagatta
     5401 catccgggaa gagttcattt gccacacgaa tggcttcgct cagatttttc tgtacaggct
     5461 ggtggtcaca gccgttcatc atcaaccatt ggttggtcga agcgtaggca cgcacatctg
     5521 acaatttttg tttccagaag gtcaaggcct catctttgtc aactgggatt tcattcccat
     5581 tactgtacca gttggcaaag agaataccta aaacacgact accatccaca ccctgccagt
     5641 acatttctga aaactgggat gtaaactgct catcttcaag gacttggttg tcaaatccaa
     5701 tcggcttcac accacgacca aaggccgcca catgaatgcc tgatttttga agaatttgag
     5761 gtgcttgtcc catatttcca aaggtatctg gaaagtagcc aatctgggtt gatttacccc
     5821 atttggcagc ttcttgttga ccaatcaagg tattgcggac attggcttca cttgaaatca
     5881 aataatcatc ctgcaagatg taaaagggac caattttaag cttgccctcg tcaatgtagc
     5941 gttggacctt gtcgcgattt tcagggcgaa tttgtaagta gtcgtcaagg acaatagttt
     6001 gtccatccaa gtggaaactc ttgaactcag ggtcattttc aaagagatca aagagattgt
     6061 caaacaattc caccaactgc atacgatgac tttcaaaagg caagtaccac tcacggtccc
     6121 agtgactatg tgagataata tgtacaacaa cattttccat gaagtaaaac ctcattctaa
     6181 atttaaattt taacgtttta aatgtaaact tgtgattctg ataagaatcg gttgagctaa
     6241 agcgagcccc ttagcgaata tccaagtaat ccaagaccaa ctcacagaac atcatgttag
     6301 cccaggagaa ccattcacga gagtagagag ttgggtcatc cacgtggaag ctttcatgca
     6361 taagacctgt accgccatcg caggcaacca gctgatccag caagaagttt ttctctgcct
     6421 tatctcttgt tgtcaagcct tggatagaaa gggcgattgg ccagatatag cgatagaagg
     6481 tatgagaact tccaagaccg ctagcgtatt ctccttggta gaaatatgga ttttcagagc
     6541 tcagaatggt acggcgagta gcttgataca cttcatcatc gaccgaacag tagcccagat
     6601 agggcgcagc tagtagactt ggtacgtttg gatcatccat aatgctggca tttcctaggc
     6661 catccacttc aaaggcataa atcttttcac ccttactgtt ggtggtgtaa gcgtagtttt
     6721 cgattccttc ttggatttca tcctgaagac gcttggcatc agcaataaca ctctggctat
     6781 cagctaggtt taatgctgcg aagatttctt gcacataacc caagactact acagcaaaca
     6841 tatttgacgg aatcaagtaa ctatactggc aacagtcatc actcggacga aaggctgacc
     6901 aagtcatacc tgtcactgca aagtcaggtc caaagccatc atttaccaag gtgtcttcct
     6961 tacggtccgt atctcggaca aaacgataag gagagttctt gtggtcttgt tcaactgtcc
     7021 atagatggag aatttcctta gtcgctgcga caaaagtctc atcaaactga ctagtctcgc
     7081 cagtttcttt ccagaggaga taagccaact gcaaaggata gcaaagcgag tccacctcat
     7141 acttgcgttc ccaaatccag ccattaaggt cggtgtggtc ggtctcgtgg tgccccttcc
     7201 agttttcctc tatgttgaag gaattggcat aaggatcctt gagtaccaag gtcatctgac
     7261 gtttgaccaa acctgcaatg gtctgacgca ggagggcatc tcttttagct acatgcaggt
     7321 agggtctgag ctgggctgtc gaatcccgaa gccacatagc aggaatatcc ccagtcaaga
     7381 caaaagttga accatcttct aagatttcaa ccgtattgtc caaggtatcg gtgtagcaac
     7441 gctcgaaaac atccacccac tctggatggt ccttagcccg ctctgctact tcatctagcc
     7501 attctctaac aatttctttc gaataaatca tcgtgcaatt ccctctttca aacaagtttc
     7561 atgttcagta tatagctttt gagacagaaa atacatacac aaatgatagt catttttcta
     7621 caaaatagtt atttttgaaa ctccttttct aaagtccctc tttttctgat ataatgtaga
     7681 aaaacagcta aaggaaagac tatgaaacca ctacttgaaa ccatcgatac ccgctttggt
     7741 actgctagca agcatgcctt ctcttgggga aataccctgc catacacagg cgtgcctttt
     7801 gggatgaatt actttgtgcc ccagaccagt gaccaggatg gatcttggtt ttttgatccg
     7861 catctgccta tctttcaggg gattcgatta actcaccagc ccagtccttg gattggggac
     7921 tactcttggc tccttctgac acctgtcacc agtcaactgg ggggagacag cctctttcac
     7981 cgccagtctt cttatgatat agataaagct tgcttccagc ctcactattt gaagctcttt
     8041 tctctgcgtt atcagattga aacccagctc acaccgactt gctacggtgc ttctattcgt
     8101 ttgaagcaaa agcaaggcaa agccctctcc ctctatcttc acgcaacaga tgaactgaca
     8161 gtagagcaag tagataagcg aactcttgcc ctgcgacaag aaggtaaaac tgaaaccaac
     8221 aaaaattcgc taacgatgtt cactgccctg caaatgaaca cggatattct tgctatcagc
     8281 caagaagctg gggactggcg aattgactta gcaagtagtc aaaccgagat gcaactagcc
     8341 acttctttca tctctccttc tcaagcactg cttaatctac ctcaagaaga ttttgatagc
     8401 tgtaaagcaa gtgcccaagc ggattgggaa aatctcctcc atcgttttga cattatagag
     8461 acaggagagg ctgaccgaac cttctttgac cactgcctct acagactctt cctcttccca
     8521 cagacttttt atgaggttga tgagtcaggg caagccatcc acatggatct ggctactggt
     8581 actgtcaagc ctggtgtcct ctttagcaac aatggtttct gggatacctt ccgcaccacc
     8641 ttccccctct ttgcccttat cataccggag cactatcaac gctttttaga aggtttcctc
     8701 aatagctacc gcgatactgg tttccttcca aaatggctgg ctccagatga acggggtatg
     8761 atgccaggta cacttttaga cggtattatc gcagatagcg cctgcaagga catggcccct
     8821 gacctagaag gagaactctt ccaagccatg ctcgaaacag ccagcaaagc cgaccctctc
     8881 ggaatcaatg gccgccacgg actagcccaa taccaagaac taggttacct ctctaccgac
     8941 caccacgaaa gtgttagcca tactctagac tatgcctata gtgacttttg tatcgccagc
     9001 tgtgccaaaa aactagagaa catagaaatc gctgaaacct acaaggctgc ttcacaaaat
     9061 taccgccagc tatttgacgc tgagacaggt tacatgcgag cacgagacaa tcaaggaaac
     9121 tttcaccctg acttctctcc ttatagttgg gggcgagact acgctgaatg ctctgccatt
     9181 caagctactt taggcgttct ccacgacatc cctggcttaa tccaactgat gggtggaaaa
     9241 gaaaccttta gcaactatct tttgaaagcc tgtcaagatg ctcccctctt tgaaacaaca
     9301 ggctatggtt acgaaattca cgaaatgagc gagatggcta ctgctccttt tggacaaata
     9361 gccatttcca accagcctag tttccacatt ccttatctct tccgctacag cgattaccct
     9421 gactacactg cccttcttat caagacgctc cgtcagaaag cttttcaccc aagttgggaa
     9481 gcctatcctg gagatgaaga caatggtagt ctctctgctt ggtacatctg gtcagctctc
     9541 ggattttatc caacctgtcc aggaaaacca agctatgacc tcggaatccc tctctttgac
     9601 catctacgag tctacctcgc taaagaagat aaatggctgg atatccatac taaacaaaac
     9661 cacaaccatt ttaactttgt caaagaatgc cgactggaca aaacactcgt atcaactatt
     9721 caacaccaag acctcttaaa agctgaacaa ctaactttca ccctcagctg gttaccaagt
     9781 cactaataac caaaagaacc tttgcatcgc gcaaaggttc ttcttttatg aaacttggac
     9841 ttgaagttga tccacaagct gaccatccac cgttaggttc agatagaaat ccaaggtttt
     9901 atctcctgcg aaatacaggg ttggtagatg caaatctttt ttgctttccc ctgcttggaa
     9961 ttgaaggact tgaatctcat cctgataggc gacaccatgg acacctgtcc ccggttgaat
    10021 cgaaatctta gcctctaaag aggagctact ttcactacgt tcaatcctaa aatgaagagt
    10081 ctctcccttt gccacagcta ggcttttttc tgcaaatgct agcccctgaa cagctactgt
    10141 ttgtgataac ctcggagttt tatagagtga aatcttggtc aacaaaggca aagcctgtga
    10201 ttctgtaatg actacacgta tcttctgtgc ttcaacaact gctcctcgta agagacgttt
    10261 gtaaccaaca gtatgacccg aaccaaactc ctgccagaca ccatccacct ctacttgcac
    10321 atgaaaagca gcgattcgtt gccctagctt caaatcttct cttaactcaa ttacatcaaa
    10381 agttttagga gaacctaagt cgagttctaa ctggatgggc aagtctgcat cgcttgccca
    10441 agagctggtc tcaaggccgt ctgtcaaatg gcgacaagca aagtctgcgg aaagagctgg
    10501 accagatacc tcagctccca gagccaaatc ttctttatag agctcattgc gataggtcgc
    10561 aaattcataa agtcgttcaa tatcctttgc atcaaagagc ccagcttgat tcggcggaat
    10621 attaagcaag agtggagttc ctcgccctac tgagtgaaag tagatttcga ccaactcctc
    10681 gagagactta ggatcctgat cctcatggta gaaccagcct ggacggatgg aaacatctgc
    10741 ctctccgatt gaaaaaatcg tgcccgaggg atccccgtgc tgaagatagt tcagctctgc
    10801 ttctgttcct agtttatcag gattcacctt ttgccacagt ggatcacctg cataccctcg
    10861 ttcattgcca atccagcgga tactggtgcc ttctgttgaa aaaatcaagc aagcgccctg
    10921 caggtcacga atggtttcaa accatttttc aaattcataa ttaacctttt gcgcgccctc
    10981 tcctctggca ccatccatcc aaacctcagc gaacttacca gcattcccat agttaggatt
    11041 tgataagatt tccttcaact gagccagata ataggcattg tagtccgctt ctcggtccac
    11101 atgatagagg ggactatggg catcccacgg tgacaggtag acccccatat ccatatcaaa
    11161 ctctgtggca gcttgggata cttcaaggag caagtcgccc tttcctctcc tccaaggact
    11221 gaccttaacc gaataatctg tgtgagctgt cggataaagg acaaagccat cgtggtgctt
    11281 gaccaccaaa atcaactttt tgaagcccgt ttccttgagc acacgaaccc actcacgcgc
    11341 atccaactga ctcgggttaa agcgctcagg atcctcctgt ccagtccccc attcttggtc
    11401 ataaaaggta ttagaaccga agtggataaa agctgctagt tcatctccca gataagctag
    11461 ctgagtctga cttggtaacg gtccatgcgg tttgattttc ttcatcgtta tatatctcct
    11521 tctttagcaa cttctcttgt tcacaaggtt atccacaggt tgtggataat aaaccatgat
    11581 ctaacagcca gctctttcca actccaactg gtaggctcag gatttttctc ctcaatattc
    11641 cctcttcaaa cagctctgtc atatggttct gctaacacct ctcagaatgc tactctccat
    11701 aattattctc aggcttcttt caggtcattg tgtgaagacc attcactctc cagacttttc
    11761 cacagattgt gaataagtga ttattctaaa ctatcacctc gtcgactata acaaaaaata
    11821 aaaagagggt caataagata attatctctt cctaaatact cagaaaagat tctataattt
    11881 gtacaattga acaagaaatg cttctccaaa atctatcttc aaaagaatgt tgcatctttt
    11941 tatcctttca cacaaacatt tctctttata cagatccaag tgataaccag atgataattg
    12001 gacacttttt ggtcatttgt atattttttg aaaaattttt taaaaaaatt ttatatcctt
    12061 tttatagaag tttatgcatt tgtatctatg tattctatcc tatgttatgt ataataacat
    12121 aaaattgtgc taaatattat aatttatatt attttccgag aaattatagc cattgacatt
    12181 acctaggaat tgcgttataa catacatgta agcggtaccc aaaatggtgc caagtcaaaa
    12241 tttttaagga ggaaaataca tgtcttcaca tccaattcag gtcttctcag aaattgggaa
    12301 actgaaaaaa gttatgttac accgtccagg caaggagtta gaaaacttgt tgccggacta
    12361 tcttgaaagg cttctttttg atgatattcc tttcttggaa gatgctcaaa aagaacatga
    12421 tgcatttgcc caagctcttc gcgatgaagg aattgaggtt ctctacctag aacaactcgc
    12481 tgctgaatca ttgacctctc cagaaatccg cgatcaattt atcgaggaat acttagacga
    12541 agccaacatc cgtgatcgtc aaaccaaggt tgctattcgt gaattgcttc acggcatcaa
    12601 ggacaaccaa gaattggttg aaaaaacaat ggctgggatt caaaaagttg aattgccaga
    12661 aattcctgac gaagctaaag atctaactga cttagttgaa tcagattatc catttgcaat
    12721 tgacccgatg ccaaacctct atttcactcg cgacccattt gcaacaattg gaaacgccgt
    12781 atcgcttaac cacatgtttg cagacactcg taaccgtgaa acactctacg gtaagtatat
    12841 cttcaaatac cacccaatct atggcggaaa agtggatttg gtctacaacc gtgaagaaga
    12901 tacgcgtatc gaaggtggag acgagctagt tctttctaaa gacgtccttg cagtaggtat
    12961 ctctcaacgt acagacgcag cttctatcga aaaacttttg gtcaacatct tcaagaaaaa
    13021 tgttggcttc aagaaagttt tggcctttga atttgctaac aaccgtaaat tcatgcactt
    13081 ggatactgtc ttcactatgg tagactatga caagttcact attcacccag aaatcgaagg
    13141 cgaccttcac gtttactcag ttacttacga aaacgaaaaa cttaaaatcg ttgaagagaa
    13201 aggtgactta gctgaacttc ttgctcaaaa ccttggtgta gaaaaagttc atttgattcg
    13261 ttgcggtggt ggcaatatcg tagcagctgc gcgtgaacaa tggaacgacg gttctaacac
    13321 tttgaccatc gcacctggtg tggtagttgt ttatgaccgc aataccgtga ccaataagat
    13381 tttggaagaa tacgggcttc gcttgattaa gattcgcgga agtgaattgg ttcggggccg
    13441 tggtggacct cgttgtatgt ctatgccatt tgaacgtgaa gaagtgtaat cgctgttcgg
    13501 cattcgtcaa tagaaaatgt aaaaaataga aagaggaaat aataaaatga caaattcagt
    13561 attccaagga cgcagcttct tagcagaaaa agactttacc cgtgcagagt tagaatacct
    13621 tattggtctt tcagctcact tgaaagattt gaaaaaacgc aatattcaac accactacct
    13681 tgctggcaag aatatcgctc tcctatttga aaaaacatct actcgtactc gtgcagcctt
    13741 tacaactgcg gctatcgacc ttggtgctca cccagaatac ctcggagcaa atgatattca
    13801 gttgggtaaa aaagaatcta ctgaagatac tgctaaagta ttgggacgta tgtttgacgg
    13861 gattgaattc cgcggattca gccaacgtat ggttgaagaa ttggcagaat tctcaggcgt
    13921 tccagtatgg aacggtctaa ctgacgaatg gcacccaact caaatgctcg ctgactactt
    13981 gactgttcaa gaaaacttcg gtcgcttgga aggcttgaca ttggtatact gtggtgatgg
    14041 acgtaacaac gttgccaaca gcttgctcgt aacaggtgct atccttggtg tcaatgttca
    14101 catcttctca ccaaaagaac tcttcccaga aaaagaaatc gttgaattgg cagaaggatt
    14161 tgctaaagaa agtggcgcac atgttctcat cactgaagat gctgatgaag cagttaaaga
    14221 tgcagacgtt ctttacacag acgtttgggt atcaatgggt gaagaagaca aattcgcaga
    14281 acgtgtagct cttcttaaac cttaccaagt caatatggac ttagttaaaa aagcaggcaa
    14341 tgaaaacttg atcttcctac actgcttgcc agcattccac gatactcaca ctgtttatgg
    14401 taaagacgtt gctgaaaaat ttggtgtaga agaaatggaa gtaacagacg aagtcttccg
    14461 cagcaagtac gctcgccact tcgatcaagc agaaaaccgt atgcacacta tcaaagctgt
    14521 tatggctgcg acacttggta acctttatat tcctaaagta taattttaga taataaaccg
    14581 tctaccaaca gctatgaggg ctgcgactaa tagctttagt ccggtcctct tttatgtaat
    14641 ggtaatctat tatttcttat aaaatatgtg aaaaatcatt aaattgaaat ctaaacgcat
    14701 tctattgagt gtgataaagg agaatttatg gcaaatcgta aaattgtagt agctttggga
    14761 ggaaatgcga ttctttcttc tgacccatca gcaaaggctc aacaagaagc tttagttgaa
    14821 acagctaagc atcttgtaaa attgattaaa aatggagatg atctgattat cactcacggt
    14881 aatggacctc aagttgggaa tctcttgctc caacatttgg catcagactc tgaaaagaac
    14941 cctgccttcc cactcgactc acttgtcgct atgacagaag gtagcatcgg tttctggttg
    15001 aaaaatgctt tgcaaaatgc tctcttggat gaaggcatcg aaaaaaacgt tgcctctgtt
    15061 gtaacgcaag ttgtcgtaga taaaaatgat ccagcttttg ttaacttgag taaaccaatc
    15121 ggtcctttct attcagaaga agaagcaaaa gcagaagccg aaaagagcgg agcgactttc
    15181 aaggaagatg ctggccgtgg ctggcgtaag gtcgttgcct caccaaaacc tgttgacatc
    15241 aaagaaattg aaaccatccg tactctttta aatagtggtc aagtcgtcgt agctgcaggt
    15301 ggtggcggta ttcccgtcgt caaagaaaac aatggacatt tgactggtgt cgaagcggtt
    15361 attgataaag acttcgcttc ccaacgtttg gcagaattgg ttgatgcaga cctcttcatc
    15421 gttttgacag gtgtagatta tgtatttgtt aactacaaca agccaaacca ggaaaaattg
    15481 gaacatgtga atgttgccca gctggaagaa tatatcaaac aagatcagtt tgcaccaggt
    15541 agcatgcttc caaaagtaga agcagctatc gcttttgtca atggtcgtcc agaaggaaaa
    15601 gcagttatta cttcccttga aaatctaggc gccttgattg aatctgaaag tggaacaatt
    15661 attgaaaaag gataagttgt tttactaata agatgtattc tatttctagt atctttatat
    15721 caaattagaa attattcttg aaaacatgta caatatttca aaagatatta gttttttaga
    15781 ctttaatatg gtaaaacaaa tataaataga aagcgttttc ttgaatgttt atttaagaaa
    15841 gtagttggtt ttttacactt tgttagacat caggaggaaa aacaaatgag tgaaaaagct
    15901 aaaaaagggt ttaagatgcc ttcatcttac accgtattat tgataatcat tgctattatg
    15961 gcagtactaa cttggtttat ccctgcgggg gcctttatag aaggtattta cgagactcag
    16021 cctcaaaatc cacaagggat ttgggatgtc ctcatggcac caattcgggc tatgctaggt
    16081 actcatccag aggaaggttc gctcattaaa gaaacgagcg cagcgattga tgtagccttc
    16141 ttcatcctta tggttggtgg tttccttggc attgtcaaca aaactggtgc tcttgacgta
    16201 gggattgcct ctatcgtgaa gaagtataag ggccgcgaaa aaatgttaat tttggtacta
    16261 atgcctttgt ttgccctcgg tggtacaact tatggtatgg gtgaagaaac aatggccttc
    16321 tatccactcc ttgtgccagt tatgatggcc gttggttttg atagcctgac tggtgttgca
    16381 attattttgc tcggttctca aatcggctgt ttggcatcta ctctgaatcc atttgcgaca
    16441 ggtattgctt cagcgactgc gggagttggt acaggggacg gtatcgtact tcgtctgatc
    16501 ttctgggtta ccttgactgc tcttagtact tggtttgttt accgttatgc ggataagatt
    16561 caaaaagatc cgactaagtc actggtttat agtactcgca aagaagattt gaaacacttt
    16621 aacgtagaag aatcttcatc tgtagaatct acacttagca gcaaacaaaa atcagttctc
    16681 ttcttatttg tgttgacatt catcttgatg gtattgagct tcattccatg gacagacctt
    16741 ggcgttacca tttttgatga ctttaatgct tggttgactg gtcttccagt tattggtaat
    16801 attgtcggtt catctacttc tgcactaggt acttggtact tcccagaagg cgcaatgctc
    16861 tttgccttta tgggtatcct gattggtgtt atttatggtc ttaaagaaga taagattatc
    16921 tcttccttca tgaatggtgc tgctgacttg ctcagtgttg ccttgatcgt agcgattgct
    16981 cgtggtattc aagttatcat gaacgacggt atgattaccg atacaatcct caactggggt
    17041 aaagaaggct tgagcggtct atcttcacaa gtctttatcg ttgtaactta tatcttctat
    17101 ctacctatgt cattcttgat cccatcttca tctggtcttg ccagcgcaac tatgggtatc
    17161 atggctccac ttggagaatt tgtaaatgtc cgtcctagct tgattatcac tgcttatcaa
    17221 tctgcttcag gtgtcttgaa cttgattgca ccaacatctg gtattgtgat gggagctctt
    17281 gcacttggac gtatcaacat tggtacttgg tggaaattca tgggcaaact cgtagtcgct
    17341 attattgtag tgaccatcgc ccttcttctc cttggaacct tccttccatt cctataaaat
    17401 agtgagtgag gtgattccat gaaaatagat ataacaaatc aagttaaaga tgaatttctt
    17461 atatcattaa aaaccttgat ttcctatcct tcagtactca atgaaggaga aaatggaaca
    17521 ccttttggac aagcaatcca agatgtccta gaaaaaactt tagagatttg tcgagacata
    17581 ggtttcacta cctatcttga ccctaaaggt tattacggat atgcagaaat cggtcaggga
    17641 gcagagcttc tggccattct ctgtcatttg gatgttgttc catcaggtga tgaagcagat
    17701 tggcagacac cgccatttga agcaactatc aaagacggct gggtattcgg acgtggtgtc
    17761 caagatgata aaggcccttc gctcgcagct ctctatgcag taaaaagctt gctggaccaa
    17821 ggtattcagt tcaaaaagcg cgtacgcttt atctttggta ccgatgagga aaacctctgg
    17881 cgctgcatgg cacgctacaa taccatcgaa gaacaggcca gtatgggctt tgcacctgac
    17941 tcatcttttc ctctgaccta tgctgaaaaa gggcttctac aggtcaaact tcatggcccg
    18001 ggatcggatc aactagagct tgaagtagga ggcgccttta acgttgtacc agacaaggcc
    18061 aactaccaag gtcttctcta tgaacaggtt tgtaacggtc tcaaagaagc tggttatgat
    18121 taccaaacca ctgaacaaac cgtaacggtt ctcggagtgc caaagcatgc taaggatgct
    18181 agtcaaggta tcaatgctgt catccgacta gctaccattc ttgctcctct ccaagaacac
    18241 cctgctctca gttttcttgc aacacaagca ggtcaagacg gcacaggaag acaaatcttt
    18301 ggtgatatag cagatgaacc ttctggtcac ctatccttta atgtcgcagg tctcatgatc
    18361 aatcatgaac gttctgaaat ccgtattgac attcggactc ctgtcttagc tgacaaggaa
    18421 gaactagtag agttgcttac aagatgtgca caaaactacc aactccgcta cgaagagttt
    18481 gactatctag cgcctctata cgtcgcaaaa gacagtaaac tcgttagcac actgatgcaa
    18541 atctaccaag aaaagactgg cgataacagt cctgctattt catccggtgg tgccactttt
    18601 gctcgcacca tgccaaattg tgtagccttc ggcgccttat tcccaggagc gaagcagaca
    18661 gaacatcagg caaatgaatg tgccgttcta gaagatttgt accgtgctat ggatatttat
    18721 gccgaagccg tctatcgact tgcaacttaa tcaggcaact gtttctacaa aaaaaaatcg
    18781 accgattaat gaactgcacc ccaaaagtta gacagaataa atctaacttt tggggtgttt
    18841 tattatgaaa ttgaattatg aagataaagt tcagatctat gaactaagaa agcaaggaca
    18901 aagcttcaaa cagctttcaa aaagatttat gtggatgttt ctggtttaaa gtcatctgaa
    18961 tctttgagat gagctttata aatcgctttt ttcagtgttt gcactggtgt ttcgataaac
    19021 tcaaactttt tagccgtggt attgcctgat tttatagtat attgaaacta gaatagtaca
    19081 cctctacttc taaaacattt ttagaaatcg atttgtcctg ttcttatttc attttactat
    19141 atttgagcca cttcgtcttt aacggcttta ttcataagct cttgtaattt ttctttacta
    19201 tcaattactt ctgattttcc gttgtaattt attgtaatag gttttaactt acctaatttc
    19261 tcgacacgct cattaatttg atcttttttg aaggctgcat atgtttttcc taagattttt
    19321 tcgaaaatat atttatcaga tagcggtttg ccttcttctt cagcttggtt tttgtattga
    19381 tttgaaacat aaggaacaaa tccttcatag taacctaatg ctcccataag ttcaaaagct
    19441 tgttttctaa ttcaaaccat tgcaactcag atttcagctt ttcagataaa tcctgctcat
    19501 ccaaataatg acttaaaatt agtgctgaac tcgtttctgt atcctgtaca ggctgatcac
    19561 ccataccatc aaaaaataaa ctcgttccta gcaagaccga acaagctcct attgcatatg
    19621 gcctcaaaga aaaacgctgc tttctctcaa attgaaattc tttcatccca tctcccatca
    19681 ttcattatta ctgtatattt tgtatatcag aaatagtttg tattcacaaa tctttctagt
    19741 tattccctta tcattcctaa ttaagggaga taacatacaa taatttttag ttaaatgtat
    19801 atcgatgttt tttgtttttc ttaataaacg caatacaaaa agagcctgtt gccaagctct
    19861 ttgtactcaa tgaaaatcaa agagcaaact aggaaactag ccacaggttg ctcaaaacac
    19921 cgttttgagg tagtagataa gactgacgaa gtcagctcaa aacactgttt tgaggttgca
    19981 gatagaactg acgaagtcag taacatctat acggcaaggc gacgctgacg tggtttgaag
    20041 agattttcga agagtattag tctattattt cttctcagcg cgaagggctg acaagatttg
    20101 tgttcggata tcatccacac catttggagt atttggtaaa aagatggttt gatttccttt
    20161 agaggcaaag gtattcaagg tatccaaata ctggttggtc aagaggatag acatgatttg
    20221 ttcttctgtc atgccaacat tggcttcctt gagttcggtg atagactctg ccaatccatc
    20281 cacaatcgcc ttacgttgtt gggcaatccc cacaccatga aggcggtctt tttctgcttc
    20341 ggcttcagct gcagtgacaa ttttaatctt gtcagcttcc gccaattctt gtgctgcgac
    20401 ccgcttacgt tgcgccgcat tgatttcatt catggattgc ttaacttctg catccggttc
    20461 gactttggta atcaaggttt tcacgataat gtagccgtaa gtggtcattt cttctgctac
    20521 ttggtgttga acctcaaggg caatctcatc ttttttctca aacaattcat ccaaggttaa
    20581 ttttggaaca gaagagcgaa gagcatcttc gatataagat ttaatctgag attctggacg
    20641 tatgagttta tagtaagcat ctgtcacgct ctgctcgttg acacggtact gagtcgctac
    20701 attcatcata acgaacacat tgtccttggt cttagtctca accacaatat cactttgcaa
    20761 caagcgcaac tgaatccgtg ctgcaatcga gtcaatccca aaaggcaagc gaatatgaat
    20821 accgctatta gcaacctttt ggtatttccc aaagcgttca ataatcgcca ccgactgctg
    20881 acgaaccaca taaactgtac tcagtgtgac tatcaccaat aggagcacac aaacaatcag
    20941 aaaaatcatg aaaaatattg ccataatgga acctccacaa gtatttttct agtattatag
    21001 cacatttaaa gaaggctgtg ccgtttttac tgcgattttt cctgaaatgt caataattag
    21061 aggtgaattg tcctattgtc gtccaatctc ttgctaaaat aactctttat aaaaggcaat
    21121 cgtttcttct aaggttggca taaatggatt tcctggtgcg caggcatcaa tcaaggcatt
    21181 cttagaaagg tattcaaagt cgaaatcttt ttcttcaata ccaagttcag tcagtttctt
    21241 aggaatacct actgtctcag aaagcttctc aatctcaaca atcgcataat cggcacattc
    21301 ttgatctgat ttaccttcta catgaagtcc caaggctttg gcaacattgc ggaaagcttc
    21361 tggtacacgt ttagcatttt cacgttctat aactggtagc aacatggcac agcaaacacc
    21421 atgtggcaaa ttatatactg caccgagttg gtgagccatt gagtgaacat aaccaagacc
    21481 agcattatta aagctcatac caccaaggaa gatggcattg accatacctt cacgcgcttc
    21541 aatatcatgt ccattttcta cagcacgagg aagatattcc ttgataagct caatagcacc
    21601 aatagacagt ttcttggtta catcataagc accaggtgtt accaaagctt caacagcgtg
    21661 agtcagagca tccataccag tagcagcagt caggccttta ggtttggaaa gcatgagttc
    21721 cgggtcatta acagagataa gagcaaggct attcttgtca accattacca tcttaacctt
    21781 gcgttcttca tcagtaatca catagttaat agtgatttct gcagaagttc ctgccgttgt
    21841 attgatagcc acaactggca agcctttttt agcagactta tgaagccctt cataatcctg
    21901 tggttttcca ccatttgtag ccatgataga aatacaacta gctgcatcct gtggagaacc
    21961 cccgccaaga ctgatgataa agtcacagcc atgttcttgc aaagcagcta ccccatctgt
    22021 gacattttta caagtagggt ttggctctac atcgctaaag atgacatatt cgattccttc
    22081 tgtatccagt ggttttaaaa ccttaggtaa aatgtcactg ccttcgatgt acttatctgt
    22141 caccaaaagt gcctttttat agccaagttc cttgatataa ggacccactt catttacaac
    22201 acctttacca ataaggttga ctgccggaac ataaaatgta gccatataat tttcctcctg
    22261 cgcctccgcg ctatctattt aacatcatta tattagtttt tgtattcgtt ttcaattatt
    22321 tgtgaattgt ttaacaaatc tttttaaaag gataggaggg acataagttc acctcctata
    22381 tacacaattt ttatttatgt agcggtccca acaactgaca tgcacgatag tctgctgatt
    22441 ctagatcttc tgttccaaat aaggaccaat ttttaggtct aaagatatct tcctcgggta
    22501 cattatgcat attgacagga attctcaaca tagaagccaa ggtaatcaag tctgctccaa
    22561 tgtgtccata tgttatggct ccgtgattag ctccccaatt attcatgacg tcatagacag
    22621 acttgaaagc accttttcct gtcaaacgtg gagcaaacca agtagttggc catcctggat
    22681 ctgtacgatt atctaaagta tggtgaacat cttcaggaag ttcaagtgta taaccttctg
    22741 caatttgtag cactggacca acccctttta gaagattgag acgtaccatt gttactggca
    22801 tatccccctt cgtcaagaaa cgagttgaga atcctcctcc acggaagtat tcgcggtttg
    22861 ctggtgggaa gtctgtattt tcaagcatag cctgcacttc actttcttcc aactcccaga
    22921 atggtttcat aacaggtttg ccatctcgag tagcttgccc tgtaccatcc aatgtacaag
    22981 aaccagagtt gattagatgt aagaagccag ctgcagcaca accctctaaa gtatgtcccg
    23041 ttacacgttt aacagcctct ggactccaat aagtgcgcac atcagcaaag atttgtggag
    23101 tatttgttaa tagataatta aagagcatag acacaccatt tagtgaatca ttctctgtcg
    23161 caaatacaaa tggttttcga ataccattcc agtcaaactg agtattgagg aaagtttcca
    23221 taaagtcccc atttgggaaa tggtctgtcc actgacgttg accttggaaa ccagctacta
    23281 aagcatggtg accaactgct tcttcctcaa aaccaagttc agcaagtctt gggttaccaa
    23341 ccattaagtc acgtccaatc atgaacatct taataacaaa ttcccattgt ctatcttttt
    23401 cttcacggct taaaacaagg tcttcacggt tatggtcgaa tccttctttt acgttttctt
    23461 tcacccattt gagcgcacgt tcgaactctt cagggtcgta aataccacgg tccatacggc
    23521 gtgtgaactc cgtcatatct accgattcat ttcgcattcc taggtattct tggaagaaat
    23581 ccggatttac aatagaacca ccaatcccca tcgaaacact ccccattgat aagtaagctg
    23641 tatctctcat caagccagtt gcaagagctg cccgcgcata acgtaaaagt ttttctttga
    23701 catcttctgg aatagctgta tcattagctt cctgaacatc tctaccataa atcccaaagg
    23761 ctggaatccc tttttgagca tgtgaagcta gtacagctgc aagatagaca gctcctgggc
    23821 gttctgtccc attaaatccc caaatagcat gaggaatatc tggagacata tccatagttt
    23881 cactaccata acaccagcat ggtgtaactg taattgttgc gcaaacattt gattttttaa
    23941 acaactcatg ggaagctgca gcctctggaa cacgaccaat ggtagatgga gaaatcacac
    24001 attccacagg ttccccatct ggatatttca atgtgcttga aatcaaatct gccacacttt
    24061 tagccatgtt cattgtttgt acttcaagtg attcgcgtac accttgacga cgaccatcaa
    24121 tagtcggacg aatcccaata cgtggatgtt gaatcatact attttcctct tatttttctg
    24181 tttctttcat actaccgatg ataattttta aaattactta taagtataaa cctctacctc
    24241 tgccaaactt aaaggaattc ctgcgtttct caaagaaatt ctaacctttt ttcccttcag
    24301 cccttttaag tctatggata gattattgct ttctaaactg tcaatatgtt gtttaaaaac
    24361 ttcatgatta tttgaatcat atataataat atcaaaattt gataatctct gaatttcagc
    24421 atctgttcta ttataaataa ttaacttttc taattcttcg gtttgtgcta aatctatctc
    24481 ccaccatgaa ggagaatctc ctttggtatg agtcactgaa tgatgaccat aatctccgtt
    24541 tttatttcca tcaactgcta gacgagaaaa accattgtaa tccgttgaac tctgtcgagt
    24601 ttctttggtt aatgcaatat tagaaagttt cttcggagtt ctctttgaac catagacttc
    24661 aacttccgct aaacttaacg gaatagctgc tgattttaat tctattttga ctagcttagc
    24721 ccctacagaa ggtactttta aagataacaa accatcaact actttatcaa aatgtcttct
    24781 aaaaacttct tctcctgatg aagatagaaa aataacatca aaattagata aacgctgagg
    24841 ttcggcatct gttctattat aaatatcaac tttagaaacc gtaaactctt ctcccagatc
    24901 gacctgccac caagcgttag aatctgcctt agtatgagtc accgaatgat gtccgtaatc
    24961 tccatcttta ttaccatcaa ctgctaaagc agctacaccc tcataattgg ttgaactttg
    25021 agtagctacc ttatttgtag agactacttt atcttctgtt attttatcta tatcctcttc
    25081 agattgctta ccatctgatt ctctaaaaac ctctacttct gctaaactca aaggcactcc
    25141 actcgttagt aatttaactt taatatacct tgctcctttt tctttgaaat ctagactaac
    25201 agattcaccc gacaaattat taacatgttt cttagcaact tcgtttctat tattgtcata
    25261 tagaatcaca tcaaaattag atagacgttg agtctcagca tctgtgcgat tataaatttt
    25321 aacaagccca actttatcca tttttttcaa atcgacttcc caccaagagg gattatctgc
    25381 cctagtgtgt gttaccgaac cagagttaaa attaccattt ctgttaccat ccacagctct
    25441 attagggtct ccattgtaat ctacagaact ttgtgttgtt ggttttgcat atgctatatt
    25501 caaattacca tcattaaatt tatctggtgt tgggaaagag actggacctg tcccctcagt
    25561 atttatagac atctctacca ttccattatg attaaccgta atggtataaa catgggtatt
    25621 ctcatcccaa ttttctgtat aagtataatg atttttatcg ccacttatat ttatctgagg
    25681 tttatgacca gtatgccctt ttaatgtaag tgttgttgtc ctaaagtcat tatctacagg
    25741 attgatggaa taatttttgc ttatccagtt cgctaactct aattcttctt tcttccaact
    25801 ttttgatgca tcaaaatttc ctgataatgc ccacatagct gtcttatctg tcctgtaatt
    25861 attcaataaa atatgtaaat tatttggatt ttctttaaca acagcgtaag tatgtggcgt
    25921 caaatctaac gataacgact ttgtattatt agtatacata ggcaacatta cttgctgatt
    25981 tttattgata ttagcattac tattataaat ataccaggaa ttacctacac gctgagcata
    26041 cccatctcct tcataaagtt ttggataaag cgagtttaaa tagttgactt tactagacaa
    26101 ttcctcacta tttttagtca aaatttttgc attagggaat atagatgaaa tcttttcctt
    26161 atcaattttc tcatgtatta caggaagaat atgatatctc ccattattat ataaaggcat
    26221 tgtttcatca ttcgaataaa gtccttgata aaatccgttt aatgaactaa tcctaccttc
    26281 tccattccaa aatacagctt ttgttctatt tacaacttct tccttacttg gagctggatt
    26341 ttgtatagca tgtctaaaga aaggaataat acctttagta aatgctggag ttggtacatc
    26401 atttgtcata aatgtatacg cggcacattc gaaattataa actgtgcctc ccccagtata
    26461 tacattcatc atttccatag caatcattga ttctggttcc gatgcatagg atctcatatc
    26521 cctagctctt ccagtttcaa atgtgtttgt ataatgtttt tcccaccatt tccatgtatc
    26581 tgttgatgag ccccagttat cacataagcc actcaaccaa aatccactaa cgatagaatc
    26641 tgtacccgca tcatctctta ttggcgtatt tttagttgcc aacaccaaat ttttatgata
    26701 tttttgactc gcttcaaaga atgtcggatc attcataata gtttcccaga accatttttc
    26761 atgatcatgc cagataaaat gcgctccata tttggcacaa acttccaaat atttagcact
    26821 atgcggagct aactggttat tgtaaatcca ataattctca atatttaaaa caccttttaa
    26881 cacactatac ttttggaatt gttcatctaa ccactctgga ggaactgtat tacgctctcc
    26941 agccgacata ataaccaaga aaactggaat gtttagactt tgagcttcct ccaacatctt
    27001 gacataccat tctctcaatt ctttcgtatc tcgtggaata caacttgttg gtttacagac
    27061 ttttgcagga tgaagttcaa tcgctgcata tggttttaca tcttcagaaa tagcttccca
    27121 tgctcccttc aatgtgttcc cccaccataa tccgttacca ttatcattgc catacaacgg
    27181 agaaagcaac aatggcgatt cattattaat cgtcgttctc atttgaacac gattatcagc
    27241 cttaactcct gctaacaatc caaaacataa catcccaata catacaaaca gtattgtgat
    27301 taattttctt tttatttttt ctttattcat gacttatacc taaaaattta actcaataat
    27361 tttttctgaa ttaaatacca attcaatatt ttgtacatca gtatttttgc catatactct
    27421 tactcttact ttttgatctt tgtttcctcc ttccaatttt aggaatgtta tatccccatt
    27481 tttccaagca aacgatacct tatatcctcc tcttactctg aaacctttca cttctccttc
    27541 tgaccaagca gaaggtaaag ctggaattag tgataaccaa ttatgatggc tctgtactaa
    27601 taattcacaa attccactca ccaaacctaa attaccatca atttgaaatg gtggatggtc
    27661 aagaaataaa ttgccaagag tcgcattatt taacaaacca ttaatctggt tataagcagg
    27721 ttcaccttga tatagtctcg caaaaaaatg aatcagccat gcagcactcc aacctgtttg
    27781 tgtactagca tgcaaaccac ttactaacca attattaatc gcttgctccc tatcctgtga
    27841 agataaaaag ttagcgtttg ataatcttct attgatagtg attttagctg cttctgctaa
    27901 ttccggagtt ttatgaatat caatctcatt ataaggataa agcccaaata gaggtgaaat
    27961 gtgtctatga ccaggctcta cttcttcata atcctctagc cattcttgta tttgcccatt
    28021 actacctatt tttgttctag gtaatttctt ttttaactcc ttcacacgac taataaaatc
    28081 cgaattgtct cctaattgtt ttgcaattcc aatgcatgaa tcacaaaaat atcttaaaat
    28141 ttgattatca attgtagatg atagacaagc atttccttca ataccatttt ttaagcgata
    28201 tttattttcc ggtgagacac ttggacctgt catcaagtag ccatccacct caaataaata
    28261 atcttcaaag aaaagaaatg cttcttttat catttcaaaa tgttccgtaa gaatacgctc
    28321 atcttggaaa tataaatagt gttcccaaat atgagtacat aaccatggaa tagttaatac
    28381 ccaaattgca gcccccatgg catgagattg gggagccgta tcgccaaaac catccgtatt
    28441 atgatgtgct gtaaaacctc tagctccata cattttctta gcggttagtc ttcccggttc
    28501 tctcattctt tcgagcatat caaataatgg atattctact tctggtaaat cacatggacc
    28561 taccatccaa taattcattt gagtattaat attaatcgta tatttagaac cccaaattgg
    28621 atttaattca tcacaccata ttccttgaag attggcaggt aaaccattcg gttgactaga
    28681 cgatattaac agatatcttc cataatgaaa taacaagtta gtcaagtagt tactatactt
    28741 tttagtgttt tcaagaagta gattcgttgg aatgctaaga caatctttac tatagtctag
    28801 tttaaaatca actctattaa attgctcctg atattttttt acatgttcat ctttttctgt
    28861 aaagtaatca atactactaa attctccctg aagagaagaa atatctatat ttccccaata
    28921 atccgtcatt gatttgagat aaagaaatac ctctgtagca ttccgaataa ctattgtctc
    28981 tcccaataca cttacttcac catccgtaac cttagaatga catactactt taaactgaac
    29041 accttttcta cctccagcag aggccgacat taaaattgta cttgaatcca gtttagatac
    29101 ttcatcatta aaccgtttat ttctacccaa attaatgttt aaatttaatg tgttttgaac
    29161 tgatgacact atacggcaac ataaaatatt cttattaaaa ctcgtaaaat attctctttt
    29221 tatttgtaaa ttacaactat taggctcaaa cacaacatta gaaatagctg tatctaaatc
    29281 tagctctctt tcatacaatg aaagagcaca agactgaata tctatatgct caatgtaaag
    29341 ttccccaagt aattcatagt ggctttgatc tcttggggta gcaaacattg ttaactttat
    29401 caattcttcg gctttctgaa tttctccatc taaaagatat tcccgaattt ttttaagatg
    29461 caatagtgag tctggattat ttctatctga ctttcctcta taccaaatag tctcatcgtt
    29521 tagttgaata cattcttttg tagctgaacc ataaatcata ccacctaaat gaccgttccc
    29581 aatcggcaag gcttcattcc aatttgaggc agctttctta taccataatt tcactcttta
    29641 cctctcttcc aaaatggagg tgatatccca aataccacct ccacactttc tactacttca
    29701 aaattcctaa aacaactagg ataactccaa tagccataac actaaagatt agtacaaccg
    29761 gattctttcc ttttctaatt aatttacaca ttaataaagt caaagccata gggataaatc
    29821 ctggaattaa ttttgtaatc atttcttgaa cagaaataac aagttccccc tgcttaaatt
    29881 ctaatccaaa attaatacca accattgatg gaatcaaacc acccactagt actagcccta
    29941 atgctgtcgc catactcgta acgcgattca atgttccttt tgtattattt tcttggataa
    30001 gactagaacc cttagtatac ccatatttca aaccgaaata tttaacagga atattaataa
    30061 tattaaacaa tattaaggcg ataaagatac ctaaagcacc gccgtcttta gaataagacg
    30121 caccaatact aaaacagata ggaactagtg tcagccagaa caaactatct cctagaccag
    30181 ccagtggccc catcaagcca actttaatac cagtaattga agctgcacct tcatttcctt
    30241 cttgttcttc catagcggaa gtaactccaa gaataaatgg tgccgctgtt tgatgagtat
    30301 tgaaaaactc aaggtgacgt ttcattgcag gagaagctga atctttgtct gggtatagtt
    30361 ttttcaatac tggaagaatt gtatatagaa aacctaggtt ctgcattctc tcatagttcc
    30421 atgaagaacc atacataaaa cttcgtttga aaactttttt aagttcaact ttagttactt
    30481 tattattcat catagatcaa gatcctcctc aacatcacta cttgaagttg tttcttgtgg
    30541 tttacttcca atcatatcgt agaagtatgc taccgcaata ccaactagtg agatcccaat
    30601 ggttgacatt cctccataca cagaacaaat gaatccaatc aacaagaata cccacatatt
    30661 tttcttgagc atcatattta aaagcatacc aaaaccaatt gaaggtagta aagcacctgc
    30721 agagttaagt ccctgcataa cgattggtgg aaccttcgca aacaagtctg ccacatattc
    30781 ccctccaagc ataatagcta ggaaaattgg tacaatttta aataacgtga ttaaaaccaa
    30841 acttgaataa tgataaaaac cgatctttga ataatctccg tttttagcat cattatcagc
    30901 ttttttcaca aaataaacat ctaaagtttt tgcaagaaca tccaactgtt gggtagcaac
    30961 tgcaattgga actgctatag cgataccagc agtttctcct tgaccagata aaataccaaa
    31021 tgcagtacca ataatcgcac ctgaaatagt atctggtgga gtgtaacctc caatacctgt
    31081 tacaccgagc caagttaatt caagagctga accaataaga acaccttggg tgaaatctcc
    31141 taagactaag ccaaccattg cacttgtaac taacggacgg tgaatcataa ataacggtcc
    31201 attatagtca atagtggcaa taatcccaac taatgtcacc agtaatgctt gtgtgaatag
    31261 catatactgt acctccttaa attattttta taaaaatttt tccaacattg taactttatc
    31321 attaggaacc atttgaattt caactctcgt tcccttatca actattttct taaaataata
    31381 tttgtcttct tcacctagag ctacacctcc cgttaccttt tcattttcct ccgttttact
    31441 catctgtcct acattgaggt actccaattt taaatttcct tcaatagaat catacacatc
    31501 ttttggattt gtatatatca gcattgttct cttttttatt ggcacagagt ttaaaacttc
    31561 cacaaaccgt tttacactaa agaaaacaat ttttaaacct actggtgcag aaatctttaa
    31621 aatagattgt cgtgttttat cttctgagat gcgatcatta acaatgataa cttgctcaat
    31681 atcatacttt tttagccacg tagtgacaac ttgaccatgt accagacggt catcaatacg
    31741 aacaaactct atagacataa atcttcttcc tcctctacgt taagttgttg tttaacatta
    31801 aacaaactat tttgagcatt gtgaacaatt tcttctaaat tgatttttga atcataattt
    31861 gatattaatt ccactaggag agggatatta aaccctgtta caatatcaac tgaatccaaa
    31921 tttaaaaacc gtgacaaagc aacattatta ggacttcctc caatcaagtc agtcaaaacg
    31981 ataacctctt gatttgagtc taacagttca tttacttgat ttttaaaata atgttcaaac
    32041 tctacaatat tctctccagg catcaaacct aatgtcctaa ctctatcatt tgttgtctca
    32101 ccaacaaaca tttctgcggt catgagagct ccgctagcaa aattaccatg actggcaact
    32161 aaaattcctc tattaaacat tttctctttc aacaactact cctttcttaa ggataatatt
    32221 agcataaagt gaagtttctc ctgtagcaac aatagcataa gctttcttac tacgttcata
    32281 aaagtcttct cttctaagat aagtaatcgt tttaagatct gtaccatgac cttcaatcat
    32341 ctgtctatag gtaccccata tcttaggaat atcatcaccc gaaacaacgt tcataaactg
    32401 aattgaacta tcgacgtaac tatctaatgg cattaaatac agaatggaat ctaataattc
    32461 tggaatattt acaccatcac aacgaattag cttatttgca catgaggcag aaggataatt
    32521 cgcgtcagct aatactattt catctccatg tcccatttcc attaaagtct tcaataaatc
    32581 tggagaaata tttttcggta tatgttttaa cattaatatc ctccctattt tctctgaccg
    32641 tatgttttaa atttttctgc cattaaatcc atctcctcat caggaagaac tactggctct
    32701 ccaaaattct tagctaaaca ataaattttt gcacaatatt caacttcttc aacaatatta
    32761 aatgcattca ataaattttg tgcacctgct aaaattccat gattcgctag taaaactgct
    32821 ctacgacctt ccattgcttt agctgcattc acagccaatt ctttcgtacc atatgttgca
    32881 tactcagcta cccgaacatc tttccctgcc actgcaatca tataatgact agccggaagt
    32941 ggttctctga gacaagctaa tactgttgca taagttgtat gagcatggat aattgcatcg
    33001 atatcatcac gagtttgata ttgaatcaaa tgcatatacc attcgctaga tggcaagcgt
    33061 tctccctcta caacatttcc attaatatcc atcactacaa tatcggattc tttgatttca
    33121 aagaaatcaa tacccgacgg ggtaattgcc atcaattgtt tttcacgatc gaaaacactg
    33181 agattcccac ctgttccttt cgtcaaatct gtttctacta gcttcttgcc atacttaatt
    33241 aattcttgtt ttacatctga cattcttgtc ctcctcatat caaaacacta aatatttttc
    33301 aattcaccaa ttatcccccc ttcttgtaca aaacttaaga agggggaggg aggcaaattt
    33361 acaaactctc aatcttttta attcttttgg gagtaataac tctctaattg tagaaactcc
    33421 tcaataacat ggtgagcttc ttccatccct tttaattgtc ccatagctat gagctgcaca
    33481 acaatattcc ccacagctgt accctcagtc gaacctgtaa gaacctcttt accagttcta
    33541 tcagcaatca tttggttaaa ataactggct ctagcacctc ctccaatcac atatatcctc
    33601 ttataaacct tatgagttag ttcttctagt aactctatcg ctttcctata cgtttcagct
    33661 aggctttcat aaacaatctt aaatagttgt ccatctgtcc actctctagt ttcatgatga
    33721 taagctagat attctgtcaa agtcttgtgc atatcagatt ctgttgcaaa ttcagttgat
    33781 tcagtatcaa tcagaggaag attttctttt tctttctcca ccattgtcct aatatcatca
    33841 aaagaatagg ctttccctct tcgttcaaat gaacgtctta gttcctctat gatccacaac
    33901 cctgtacaat tcttcagaaa tgtaatcact ccatctttac cgacttcatt tgtaaatcca
    33961 taactgaagg attcggtagt aagaatcggt gaagtaagtt ccactccaac caaagaccaa
    34021 gtacctgatg aaataaataa actaccttct gtcttaggta ctgagacaat cgcgcttgct
    34081 gtatcatgac tacaaacatt cacaacagga atatcgccta aaccatactc cttttttatc
    34141 cttccaagaa catttccctc tgaaacaatt tcaggaagta aagatgaatt caattcaaat
    34201 agttttaaga tattctgatt ccaattttga ctcctaggat caaataattg agttgttgaa
    34261 gcaatgcttt tttctgtagc aaacttacct gtcaagagat aattaaacaa atctggcatt
    34321 aaaagaatct tattggtctt atagaaagag tcaggagatt cttgacgtgc cttaaagagt
    34381 tgaaacaagg tatttatctc cataatctga tttcctgtct ctgaatacag tttttctaat
    34441 tcagtcattt cagatatttc ctttaacact ccctttgttc tttcatcacg ataatgaaca
    34501 ggttgtgata acagcttacc ttcattatca atcaggccaa aatcaactcc ccatgtgtca
    34561 ataccgatag ataaaatctt gtaactagta ttagccaagc ggatactttc aagaatttta
    34621 gctagtagaa agtcaacatc ccaagataaa tgccctttta ctctaatagg tagattagaa
    34681 aagcgattta tttcttccat tactagttta ttttcagaaa ggtaaccaac gattgctctt
    34741 ccagaagtcg cacctaaatc tattgctaaa accgcttcca tttttcttct ctcttcgtcc
    34801 ttgattaagt ttattataat ccccacattt tattttttca atatgtgaac gcttacttgg
    34861 ataatgttca aatgttaacc caatattcat aaaaataatt caaattcctc caatttgtgc
    34921 tataatatag ttgaaatgat aaattaggag gagcttatga taaaagatga acgtgtactt
    34981 gaattgattg aaattatcaa aaagaaaaaa agaattgccg taaaagagct ggcagaaatc
    35041 actttctcca gcacaagtac cttacgtcgt gatttaattt tcttagaaaa tcaaggtctt
    35101 atcaaaagaa agcacggata cgtgaccctg tcctctatga acacaattga actttctcat
    35161 caaatacgtg aaggagaaag tactaggcaa aaaagactaa tcgctagtct cgctaaagac
    35221 tttattcggt ctggtatgtg tatctatctg gattccagta ctactgtcta cgaactctgt
    35281 ccctatcttt ctgaacttga taatttgatt atttttacaa atggtttaca tactgcacaa
    35341 accctatctg aaactgttaa agatagctcc aaaatcttta tcacatctgg cgaggtcaaa
    35401 catcaatcct gttccgtggt caactatgat aaggaaaatt ctttattaga tcattttaat
    35461 atcgatttag cattttgttc agcaagaggt attgatgacc aatatgttta tgaagcttct
    35521 ctcagccaag ctatttcaaa aaagaatatt attgacaaag cccatgaaac catcttactg
    35581 attgatagtt ctaaatttta caagactgga ttttttaaaa ttaatcccct ctccaaatac
    35641 acaaccttta tttcagacac cgtgccagat caaaaattat tagacgcagt cgaattattt
    35701 gatggagaat gggtttctga tattcaatga aaatcaaata ggaagctagt ctcagcttgc
    35761 tcaaagtaca gatttgaaaa ctgatgaagt cagctcgaaa cactgttttg aggttgtaga
    35821 tagaactgat gaagtcagtc acatatatac ggaaagacga agctgacatg atgtgaagag
    35881 attttcgaag agtattcgtt ctgtctgctg actgatttac aaatattcac cacatgacat
    35941 tattttctta gcaaccccaa aaacagaggc tggacaaaaa ctcaatttca ggagaaaatg
    36001 gagtaaacct tcccacaata aaacgcatag tatcaagttt ttgcacacct gatattatgc
    36061 gtttttctga ttttaaagac tttgtcgcct agaccctata tttcaagaat taagtgacct
    36121 tgatgatcta aatttattgt ggtatactat ttttgtcatc ggttaccgat tacgttccta
    36181 tgggttcgtg agaggaggtg aaactaatga gccttatcat agagctcgct cttactatta
    36241 tagcggatgt tatagctgga attatcttgt atttcgtctg cagatggcta gatagtaagg
    36301 agtagaccga ctgactagcc tataaacacc cgttaaatcg ctaagatacg tcaaaaaagc
    36361 ccttaactac ggccatagtt aggggctttg gtgttctaat gaaccttatc aattaactac
    36421 attttagcat ataatctaca atagttcaag cattaagtga ggttgatgat ctaaatttag
    36481 tgtggtatac tatttttgtc atcggttacc gattacgttc ctatgggttc gtgagaggag
    36541 gtgaaactaa tgagccttat accagagctt gctcttacta ttatagcaga tgttatagct
    36601 ggaattatct tgtatttcgt ctgcaaatgg ctagatggta agaagtagac cgactgacta
    36661 gcctataaac acccattaaa tcgctaagat acgtcaaaaa agcccttaac tatggcacta
    36721 gttaggggct ttggtgttct aatgaacctt atacactaac tacattctag catataagcc
    36781 cagatatttc aagagtttta tttatttttt caggttccct tagttctgaa aggtctataa
    36841 tgaagttagc catctagtat caaaaaaccg actagctctt atgaactagt cgatttctca
    36901 tcaatgcgcc aacatttctt gggcgatttc ttggccagat aggttatctg ggtagtaggt
    36961 tggccagttg tccatttctt caaagagggc ttcttggctt gtgcctccaa agaagatatg
    37021 gaaatgttct gccttaactg gggcaacatt gtggtcacta aactgaacat acttgaattg
    37081 tccagcgtca gcatctgtgg cttcaaagag gaaacgcacg ccacgattgc ctttcttgta
    37141 agtcaaaatt ttcttaccga catacttgta agtgtatttc ttgctttgtc caccttgaac
    37201 aaattccata gtattatcag taatgttaat cttagtcaca tctgtctgat agccttttgt
    37261 atagtaagcc ttgtactcag cctgggtcat cttaccagtt aacttagcct tgtagtcaaa
    37321 gacttggtca aacgtgccgt cttcaaggaa aggataaact gattgccagt tacctgcata
    37381 gtcactcaag gtacggtcct tgacagctgc atcctcgaag taaccatttt ggactgtctt
    37441 ggtatcctct gccttttcag gttcgattgc tgggccttct tggtctgttg tttgtttcaa
    37501 agccttgagg tttttctcca tcacggaaat gtagttttct ccagccttgg tgtcctcttc
    37561 tgtcagactt tctaaaggat tgaggacatc agttttgaca cctgcttctt ttgaaagtgt
    37621 gttagcaagg gcttgtgagg cattttcttc aaaatagata taggcgattt tatttttctt
    37681 gacatactct gtcaattctg ccaagcgagc agctgatggc tctgcatctg gagaaagtcc
    37741 tgagattgcg acttgtttga gtccatagtc caaggcaaga tagttaaagg ctgcgtgttg
    37801 agtcacaaag ctcttttgtt ttgcttgaga caagccttct gcgtaagcct tatccaaggc
    37861 ttgcaatttt tcgatatagg cagctgcatt cttctcaaag gtctcttttt tatcaggata
    37921 atctgctgac aagctgtcgc ggatgtgctc tactagttta atggcacgaa ctggtgataa
    37981 ccaaacatgg gggtcaaact catggtgatg accttcttct ccatggtcat gatctccctc
    38041 ttcttcctcg ccacctggca agagcaacat atcgcctgtc gccttgatgg ttttcacttt
    38101 tttcttatcc aaggtatcta gcaatttagg tacccatgtt tccatgtttt cattttcata
    38161 aacaaaggta tctgcatctt ggattttggc aactgccttg gcagatggtt cgtattcatg
    38221 aggttctgtc ccagcaccga ttaggagttc tacattagcc gtatctcctg cgacttgctt
    38281 ggtaaattca tagacagggt aaaaggttgt cacgatattg agtttaccat ctgcctgttt
    38341 ttgattggaa caagccacta aaaacaaggc acatagactg gctagtaata agctaatttt
    38401 tttcacgttc gtctcctatt tgataaaacg tcttactaaa ctgattagta taaagacagt
    38461 tacaaaaata atggtaatac ttgcacttgc aggtgtttct gcatagtagg aaatgtaaag
    38521 tcctgctacc attcccaaaa agccaatcgc actggcaagc agcataaccg atttaaagtt
    38581 tttccccaga cgcagggcaa tactagctgg caagaccata atggtcgata ccagaagagc
    38641 tcctgctgca ggaatcataa gggcaatagc cacccctgtc accatgttaa aaagaatgga
    38701 catggtacga actggcaagc catccacaaa ggccgtatct tcgtcaaaag ttaagatata
    38761 cataggacga agaaagagaa aggtcaaaat caaaacaacc gccgcaatga caaagaggga
    38821 aatgacctgt tcttcactga tagtcacgat cgaaccaaag agatattggt ccaaactcat
    38881 tgaactcgag cttttaccct tgctcatgac aatcagagaa acagccagac ctgttgacat
    38941 gaggatagct gtcccgattt ccataaagct cttgtaaacc gtacggagat actccagaaa
    39001 gaccgccgca atcaagacaa tggcaatagt agaaacagtt ggagaaatcc ccaaaaccag
    39061 accaaaggct acacctgaaa gtgagacgtg gctaagggta tcactcatca aactctgacg
    39121 acgcaagatg aggaaggttc ccaataccgg tgagaaaaga ctcatagcaa taaccgccaa
    39181 aaaggcgcgt tgtataaagt cgtaagataa taaactaagc atggcccacc tcctggccat
    39241 tctcatgaac gttgaaacaa cgccatggcg agtcttggtt acggactaga tgaatattgc
    39301 gatccgcata atccttaact tcttcagggt catgggtaat catcaaaaca gccttgccat
    39361 gatgatgggc gctgtggtgc atgagttcgt aaaattcatt tttacttcct gcatccatcc
    39421 ccgttgtcgg ctcgtctagg ataaacacat cagggtcaga agcaaacata cgcgcaatta
    39481 ccgctcgctg cttttgtccc ccagatagag accccaagcg tttgtctcga tgttcccaca
    39541 tgccaactga gtccagactg gccttgatat gctcctcatc atgagcattc aaacgacgga
    39601 accagccttt tctcggatag cgacccgact tgacaaattc atagaccgta cttggaaaac
    39661 cagcattaaa actggcaatt tgttgaggaa gataggctat tctcaatttc ttaccttgcg
    39721 tatttgtctt tgaaatagcc acctttccaa tgcgtggttg cagaattcca agactagcct
    39781 tgatgagcgt cgtcttagcc gctccatttt ccccagtcaa ggtaacaaat tccccactat
    39841 caacacaata attgatatgt tcaagaacag gctccttatc ataatagaag gacaaatcct
    39901 ctaccgtaat atatctcatt atttgatttc tcctactaaa gcagtcaaaa accgctgaat
    39961 cactttttgt tcatttggag taaactgagt cgccacttgt tcataggtta aaagtgtatg
    40021 ctcatggtga tggtggtgct cctcagcgat tggacgagcc aagtcagtca actgataaaa
    40081 aatcacacgc gcatctttag aatctttaga tgtttccaac atcccttcct tgaccaaaga
    40141 cttaatggcc ttggtaactg ccgcctgact gacattgaga cgacgggcca attctgaatt
    40201 tgttaaagat tcctctgaca agagcataag gatatgctcc tgagtattgg tcagggccac
    40261 ctcgctagtg caatgaccta ttaggatttc atgctgattt tccgcctgca aaatcacctc
    40321 attcaaaaaa gcattgatat cctttgctag ctgtctcata tctgactcct ttccttttag
    40381 acttctcttt tttaagagaa aaatactatt ctttgacatt ttgtttacca gttaattata
    40441 tcacaagcaa aaaaagagtc aagaaaaaac gtgaaaacta gtttcattct tgaactcttc
    40501 tatattatat tatctattga aattctttga catctccatc ataagtcgcc caatctttgc
    40561 tgaaaaagcg ctcattcaga tggtaagtcg gagctggtgt gggattggat aggaaaggat
    40621 caactgcctt gtcaaaagcc aaccaaccca accaaccaag gtgaatggtg tccttcataa
    40681 agaaaggctc cccgccgtcc ttagaaaaat ctgctatatt ggtaaaacct tgactttcta
    40741 actggtagcg aatcttctgc accgtttgtt ggtacatatc ctctcgtaga ccagcatagt
    40801 ccatccattt tttattaaca ggtggaatga taaaaatcgg gtttacctta gatttagaaa
    40861 actgtgttaa aaccaactgc aagtcattat actctggcga cttgagatag gtaaagcttt
    40921 tctgagaatc ctttaatttc ttcaaatcct tcttgatctg ctcattatag aaataatttt
    40981 ccattcccat ctcattattg gaagtatttt tttcagcatc tgctttgaca acatcttcta
    41041 ttgcttgata agaaaactgg tctggcaaga tttttaaata cttagctaca tgcttatcgt
    41101 agttaacata gcctctaacc gaaaactgac caaaaaagga ggcttggcgt tcattaaaac
    41161 gagccaataa ttcaatcatt tcattgtctg ctgtcgacaa ttctccttta cttgccaact
    41221 tctgaaccag gtccttcata gctacgtttg ggaactgttg cagtaagcga gtcgctgcat
    41281 attgactagc ctgatcccca gattgatgtt tcagaaaact agtcaactgg tctccattaa
    41341 aatactgctg gaaggctgct ggatcatagc catttttact gaaccactga ggtgagataa
    41401 catacacaac ttgtttattc tccagctgtg gtaacatctg ttgcattcca aaatattggt
    41461 taagcgatgc agctcccccc tgtcctaaaa gataaggacg gtaggaacga ttgtatttct
    41521 cagctaatac cgcaggatga gcaccgtcaa aacgaagcca ttcactagag ccaaagaagg
    41581 gaacaaaacg cacatttgga tcagatagtg ctctgacttt ttgacttcgc tccttaaaac
    41641 tatcgatagt agtagccact gctgaacgct tttcagctcc tagattatga tgcatctcag
    41701 taggataaaa gaaaatgagc agaaaaacca acaaaccagc gatcaagacc ggtccgaaga
    41761 tcatccataa gcgtttaagc attttgtagc tccacaatac cagctatgat tttattagct
    41821 gtattccagt cgtcacgacc aaactctgtt acagggacac gaatgtcaaa acggttctca
    41881 atctccacaa tcaactcaac cgttcccata ctatccaaga cacctgcatc aaaaagatct
    41941 tcatccatca tgtcagaaac atcttccata aacaactcat caataatttc aataacttct
    42001 gatttgatat ccatatttta tttcctttta ttttttaaac catagattat tcaagaatcc
    42061 agaaaagatt aagaatgaca acatgacaac atggaaagtg acaaccatgc caagcaactg
    42121 aatccagcga ttctcaggta gggcagcctt ccctgctttt ttccgttcct tattgagcgt
    42181 ttttttcttg cgaacccagg catcattgat gaccaagcct agtccatgaa agagtccata
    42241 ggcgatatag taccaggtca caccatgcca aaatcccata atcagcatat ttacaatgta
    42301 agccatgctt gaggttacat tacgattttt aaagactttc tttctggtta acaccatcac
    42361 cattcgcata aagacaaagt cacggaacca gaaggacaga ctcatatgcc agcgattcca
    42421 aaactccttt aaatcccttg ataaaaaggg cttgttaaag ttgatagggc tacggattcc
    42481 catcaagttt gagatggcca aagcaaacat agaataacct gcaaagtcaa agaagagctc
    42541 cagaccaaaa gtatacataa ctgccaaggc atagagatta aagaagccac ctgactgcaa
    42601 ggctaaattc ttcagaggag gtagtaaggt ctctcctaaa acatgagcta ggataaactt
    42661 atacaaaaag ccccacatga tatagcggac agattcatcc agcatatcca tcaactcatc
    42721 tcgctcagga atggtctgat aattttcatt aaatcgctta aagcgatcga ttggaccact
    42781 cgagaaagtt ggcatgaaga gaaggaaacg gaggaattcc cagagggtaa aatccttaat
    42841 cactccatct ctcagctcga tgataatccc aaccgaacga aaggtcaggt aagaaattcc
    42901 caagaaccca agcaaagact gcgttccatt gatagctggt tgcaccttga caaagataat
    42961 cggaagtagg gacagaaaac taactaagta gaagacccac ttgccatcct tgctttttcg
    43021 ataatgcttg tagaaaagca ggagcaatat ttcccagcaa aggtaaatac ccaaggcagc
    43081 tagttgattg gtctttccac ccaccaacat ggtgacaata aagaagagac ttaccaacac
    43141 ttcataccag gcaaagcgtt tcttgaaaaa gaggcctata aagatgggca aggttgcagc
    43201 aatcacataa acaaaatact gaggattgcc atatggctct aaatgaggaa gctgttgaaa
    43261 aaactccatc atctcttatt cacctcgtta atcaatcctt tgatgtcaat ctttccattt
    43321 ggagttagtg gcaaactgtc tcggtaaagg aatttagatg gcatcatgta ggacatcatg
    43381 atgtctgtca ggtcttcctt gatggccttg gtaatatcga tatctcgctc aaactgctca
    43441 cgaacaccgt cttttaagat gacataagcc aatagatttt gtaccttatg gtccttgtta
    43501 tagcgcggta ctgcgacagc agattcgata aagcgagact tgttgaggtt ttgagagaca
    43561 tcttctaact caatgcggta accgttaaac ttaatctgga agtccatgcg tccgccgtaa
    43621 agaagcaagc cctcatctgt catggttccc acatcgcctg tgtgataggc tggcagacct
    43681 tcaaactcaa agaaggcttc tgctgttttt tcaggattgt tcatataacc ttttgaaaca
    43741 gctggcccag aaacaatgat ttctccctgt tcgccatttg gcagtttatt tccttcctcg
    43801 tcaatgataa aggttggaga atcagccttg gtatagccga ttggtaggcg tttgagagtc
    43861 gctaacatct cgtctgtcac ggcaactgct gacagagcta ctgtcgcttc tgttgggccg
    43921 taagcattga tgatacgggc atttgggaaa cgctcgcgca gtttttgagc tgttttgacc
    43981 gtcaattctt caccatcaaa gtagaaatgc gtgattccag gcattttctc actgttgaag
    44041 tattcagaca acatggccat atctgcaaag gatggtgttg atgtccagat agcgattggc
    44101 aatgaaaaga tagccgcaaa gagttgctta aagtcctgag tgatgactga aggaagagtg
    44161 aaaagcgtac caccaagtgc caaggtcggt gcccaataca tgacagacaa gtcaaaagaa
    44221 taaggtggct gtgccagcat ttgcggacga ctcggtgtcg caaattcctt atccgtaatc
    44281 atccagtttg taaagctgag gagattatca tgtgaaatct gcactccctt aggcttacca
    44341 gtcgtaccag aagtaaagat aatgtagtaa ttatcatctc ccttgactgg atgcgtgatt
    44401 tcatagttgt tcccttgggc aaaggcttct tgaacctgag ctagatttat cattggtgta
    44461 gaaacctgct ccaagggaaa ggctgaaatg gcaataatca agcttggctc tgctacttct
    44521 aaaatagctg aaactcgctc caaggccgaa tggctatcaa ttggaatgta ggcatgacct
    44581 gacttagtca gcgctacaaa ggttgccaac atttcatatt cttggccacc aaaaacaacc
    44641 acaggagact tctcaggcaa gcctagttgg tcaatgactg cagccaaact atccgaatca
    44701 gcctttaaat cgccataagt gtgttcctgc cccaaaacat tatagacagg atagctaggc
    44761 tgtgtctgag caaaatgctc aatggtttca atcatatctg ctattggttt atttgacaca
    44821 atagggattc tccttcaagt taaaattcat tatagataaa gcttccttga ccctgaccaa
    44881 gatagctaaa gaagtaaagc agccctagaa agataagaaa atacaaggct gtccgaccaa
    44941 gaaagaggta caattctttt ctctgtttca tcaagaaaaa ccattcattt ctgtaatttt
    45001 tcgctaaaat aagagtgatt cttactagct tatttttcta ccattgtacc actttatata
    45061 gtatcttttc aattgtttac cgtatgtttc caatagattt cagcttattt taaggattat
    45121 acagtttttc tatgtatatt ttcaaataga gtgatcctgc ttcaaaactc catttcagga
    45181 gacaatgaag taaatcttcc cataataaaa cacacaatat caagtttttt caacacctga
    45241 tactatgcgc ttttctgatt tttaaagact ttttaaccac tctctcattt aaaataatct
    45301 cgtctgatat aaattaaaat agcttctatc atcagacaaa tggctgatag ccaaaaactg
    45361 atgctaatac caaaactctc agtaatatag ctcattagca aaacaaatac tgaaaatgct
    45421 aatgtagaaa tcacttcaag aacggaatag acattaacta aatgattttc ctctactgtt
    45481 tcctgaagaa atacactttc aggaacttct tttagttgcg ataacatacc aactaaagct
    45541 gaaaataata aaaacatctg tgcgtttgga aaatatagaa tagtcagtgt cactattgcc
    45601 atagctacaa gaggaaaaag aatactttcc catttagcag caaggaactt ttcagatagc
    45661 ctaaaagcaa ttaagccact aattataata ccaatagagt atgctgtatt agaatatccc
    45721 cagtaacttt ccgttttatt taataactcc gttacaaaaa caagtataat ggaagaaacc
    45781 caaatcgtat ttgaaaaaat ttccaataaa tttgctgata caaaaagtct taatctagga
    45841 tttctagcaa ctaacttcca acctttgagc aaaatttcaa gattagtttc tgactctaac
    45901 acctccactt cagcgttagg aagaaataac atcagaaagc tagaaatgat atacaagact
    45961 aaattgatac acgtggtagg taacagacca attgttgcaa acaagagtcc acctaatccc
    46021 caacctatca attgaacagc ttcaccagtc attgataagg ctgaattagc cttacccaaa
    46081 tcggtcgcat agcgtggcac aatagcatag gaaacgggtg ctgcaaaacc atctagtatg
    46141 gaaattgcaa caacaaatag ataggtcacc aaaggcgcta cggattgcat tacggtaaac
    46201 attcctacca gtatcgccaa taatatagtc tttccaaatt gagataaaga taaaacccta
    46261 tttagcgcta accttttagt aaccaacgga actaaaagac tcgcaacaaa agaggatatt
    46321 cctattaaga taggaactag tgatgtggca attactgatt ttgaaataat gtatatgtta
    46381 gcaatgattg ttactctaaa gaaaatatct gctaaatttg caaataattg agagactaac
    46441 aacttgacaa atgaattaga catgtagcgc tcccccttta gttacaaact ttggaattaa
    46501 atttgatttt taatatttta gcataaaata gcagaaaaca aaagtgaggt tttttacatg
    46561 aaaaaacaaa aactattgcc tttattattc ctaattttag gaggaattat gattatcgtt
    46621 gttggctagt actaagaagt tagttagtag aataattaca tcttcaatcg aaaaagagac
    46681 tggaaaaagt cttaaaaatc agaaaaacgc tcaaatcctt tggcgctcta agcttttcta
    46741 atgatagatt tagatagtca aaaaaatatc tgaaaagttc ccaggatcgt cgtctcagca
    46801 tcaaaaatac gtgaaatttg cataattaaa ataaatttca ggggagttaa tcgagtaggg
    46861 gataatctct agcccctctc acaccaccgt acgtgccgtt cggcatacag cggttcaact
    46921 aacttttaac gcatgtcgtt caaggtaata atccaaacac gaaaccagtc cacgtttttc
    46981 caggactggt tttgatatag cacgtttaag taccgacttc tgagctacta attgataatg
    47041 gtcgccccag ccagatacct tatctgctat ccatttagga actcctaact taagcaatcc
    47101 ccataatcgt ctcgatttct tcttccattg cttccagata atcactcgta ggcgagtacg
    47161 caagcgctca tctatgctgg cgactatact tttcatattt cccaatgaga aatagtttat
    47221 ccatcctcga atagacaaat tcagttgctc aatacgtctt gttaggtcta tactccattt
    47281 cctctgtgtt agtttcttca atttaagctt aaatctccga acactatctt gatgtggacg
    47341 acttttccaa ccatctgatg atttccagaa cccaaaacct agatatttca actctcttgg
    47401 tctggtaatc ttagtcttgg tcatgtttac tttcaaacct agccgtttct caataaaacg
    47461 actgactgaa tacatcacac gcttagcggc tgcctcgctt ccgaccgtat cacacaatca
    47521 tctgcgtagc gcacaaatcg aagtcccctc ttttctaatt ccttgtccaa ttcattaagc
    47581 atgatattgg ataagagagg agataaattt cctccctgtg gtgtaccaac tagtgtttta
    47641 taacgttgac cattaatgat aacacctgaa tgaagatact tacgaatcaa ggattccgta
    47701 tctccgtctt cgataatgtt atgtactaag gacatcaatc tatcttgagg aactgtatcg
    47761 aaaaatttct ctaggtctat atccactatc cactcatagc cgtcatttaa gtattctaag
    47821 agcttcatga tggctttttc acatgaccta tttggtctga aaccataact cgtatctgag
    47881 aaatggggtt cacaaatggg gctcatgact tggacaatgg cctgttgaat cattctatcc
    47941 ataactgttg aaattcctag ttgacggatg cctccgtctg gtttaggtat ctcaacttta
    48001 agaactggtt gaggcttata ttttctctgt tttatcagtt ccttagtcaa gcgccagttt
    48061 tgtctgagat aattatccat ctcttcgata gtcattccat caatcccagc tgagccttta
    48121 ttggatttta cttgattgta ggcttccagc atattttcgc gtgataatat cttatctagc
    48181 agttttgaca tgtgcgtatt cctttcttgt tttggtgcct gagggacatc ttatattggt
    48241 gaaccttcat ctagtcatta cgtgtcaaag tccagttcta tcagaccact cattttacag
    48301 acacaagtga agtaggatta cttcgatctg tcgttttctc tttctagcga cagacctcaa
    48361 acagtctctc cccagactgt ttgagtattc accccttcgc tccacttcca ctacagaagt
    48421 ttcctcacta ctatgggctc ggctgacttc tcatgattcg ttgttactac gctttcagcg
    48481 ctcatgaaac ctcacgggat aagtcgtaca tctttcctcg tttatacaag atatgaggac
    48541 gttaaaaggt tacagtgaaa ataggaaatc tgacgcagag tctttagact ctagaaagat
    48601 ttatcttttt cacacaaacc ttagtccgaa ttcaattact ctcgtgattt acacatatag
    48661 gttacgacta cctttttgga ctttagagtt tgaagcctcc ttatccgcta tatacgcctt
    48721 actatcacgt tcctgttcgt agagccacga tttcgctatc ccttcctctg acgattctta
    48781 tttcctgcgt cagacttaaa acggtctatc cccagaccgt tttaatccgc tacctcacga
    48841 tagtcaggct tgggaatcgc tattgggttc accg
//
LOCUS       DAVKMU010000010        47461 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKMU010000010 DAVKMU010000000
VERSION     DAVKMU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.010"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            47..394
                     /locus_tag="VW343_000644"
     CDS             47..394
                     /locus_tag="VW343_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9755242.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEIEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(511..1644)
                     /locus_tag="VW343_000645"
                     /pseudo
     CDS             complement(511..1644)
                     /locus_tag="VW343_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1709..1990
                     /locus_tag="VW343_000646"
     CDS             1709..1990
                     /locus_tag="VW343_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9755243.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            2122..3066
                     /gene="manA"
                     /locus_tag="VW343_000647"
     CDS             2122..3066
                     /gene="manA"
                     /locus_tag="VW343_000647"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9755244.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(3106..4441)
                     /locus_tag="VW343_000648"
                     /pseudo
     CDS             complement(3106..4441)
                     /locus_tag="VW343_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            4669..5418
                     /locus_tag="VW343_000649"
     CDS             4669..5418
                     /locus_tag="VW343_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9755245.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(5438..5893)
                     /locus_tag="VW343_000650"
     CDS             complement(5438..5893)
                     /locus_tag="VW343_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9755246.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(5913..7088)
                     /locus_tag="VW343_000651"
     CDS             complement(5913..7088)
                     /locus_tag="VW343_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9755247.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(7147..7992)
                     /locus_tag="VW343_000652"
     CDS             complement(7147..7992)
                     /locus_tag="VW343_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9755248.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            8117..8674
                     /locus_tag="VW343_000653"
     CDS             8117..8674
                     /locus_tag="VW343_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9755249.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            8693..9160
                     /locus_tag="VW343_000654"
     CDS             8693..9160
                     /locus_tag="VW343_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9755250.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            9249..9878
                     /gene="upp"
                     /locus_tag="VW343_000655"
     CDS             9249..9878
                     /gene="upp"
                     /locus_tag="VW343_000655"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9755251.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            10052..10642
                     /gene="clpP"
                     /locus_tag="VW343_000656"
     CDS             10052..10642
                     /gene="clpP"
                     /locus_tag="VW343_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9755252.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            10721..10969
                     /locus_tag="VW343_000657"
     CDS             10721..10969
                     /locus_tag="VW343_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9755253.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            11070..12230
                     /locus_tag="VW343_000658"
     CDS             11070..12230
                     /locus_tag="VW343_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9755254.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGRDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            12498..13367
                     /locus_tag="VW343_000659"
     CDS             12498..13367
                     /locus_tag="VW343_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9755255.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGAISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFEMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            13371..14327
                     /locus_tag="VW343_000660"
     CDS             13371..14327
                     /locus_tag="VW343_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9755256.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            14327..15091
                     /locus_tag="VW343_000661"
     CDS             14327..15091
                     /locus_tag="VW343_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755257.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKDKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            15091..15801
                     /locus_tag="VW343_000662"
     CDS             15091..15801
                     /locus_tag="VW343_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755258.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            16160..16816
                     /locus_tag="VW343_000663"
     CDS             16160..16816
                     /locus_tag="VW343_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9755259.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            16924..18019
                     /gene="prfB"
                     /locus_tag="VW343_000664"
     CDS             join(16924..16995,16997..18019)
                     /gene="prfB"
                     /locus_tag="VW343_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9755260.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            18037..18729
                     /gene="ftsE"
                     /locus_tag="VW343_000665"
     CDS             18037..18729
                     /gene="ftsE"
                     /locus_tag="VW343_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9755261.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            18722..19648
                     /locus_tag="VW343_000666"
     CDS             18722..19648
                     /locus_tag="VW343_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755262.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYYKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            19934..22114
                     /locus_tag="VW343_000667"
     CDS             19934..22114
                     /locus_tag="VW343_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9755263.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            22166..22981
                     /locus_tag="VW343_000668"
     CDS             22166..22981
                     /locus_tag="VW343_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9755264.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYNRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            23121..24464
                     /locus_tag="VW343_000669"
     CDS             23121..24464
                     /locus_tag="VW343_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9755265.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDRNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            24841..26031
                     /locus_tag="VW343_000670"
     CDS             24841..26031
                     /locus_tag="VW343_000670"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9755266.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            26584..27519
                     /locus_tag="VW343_000671"
     CDS             26584..27519
                     /locus_tag="VW343_000671"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9755267.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            27553..28590
                     /locus_tag="VW343_000672"
     CDS             27553..28590
                     /locus_tag="VW343_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9755268.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            28763..29368
                     /locus_tag="VW343_000673"
     CDS             28763..29368
                     /locus_tag="VW343_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9755269.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            29525..30055
                     /locus_tag="VW343_000674"
     CDS             29525..30055
                     /locus_tag="VW343_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9755270.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            30076..31161
                     /gene="rlmN"
                     /locus_tag="VW343_000675"
     CDS             30076..31161
                     /gene="rlmN"
                     /locus_tag="VW343_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9755271.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITISTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            31161..31643
                     /locus_tag="VW343_000676"
     CDS             31161..31643
                     /locus_tag="VW343_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9755272.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            31645..33186
                     /locus_tag="VW343_000677"
     CDS             31645..33186
                     /locus_tag="VW343_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9755273.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(33242..34045)
                     /locus_tag="VW343_000678"
     CDS             complement(33242..34045)
                     /locus_tag="VW343_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9755274.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34128..34349)
                     /locus_tag="VW343_000679"
     CDS             complement(34128..34349)
                     /locus_tag="VW343_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755275.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            complement(34828..35025)
                     /locus_tag="VW343_000680"
     CDS             complement(34828..35025)
                     /locus_tag="VW343_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755276.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKRNMDLLADD
                     SLAGDIILLWRIQFGTFTTET"
     gene            35195..35467
                     /gene="rpsP"
                     /locus_tag="VW343_000681"
     CDS             35195..35467
                     /gene="rpsP"
                     /locus_tag="VW343_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9755277.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            35487..35726
                     /locus_tag="VW343_000682"
     CDS             35487..35726
                     /locus_tag="VW343_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9755278.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            36303..37091
                     /locus_tag="VW343_000683"
     CDS             36303..37091
                     /locus_tag="VW343_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9755279.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
     gene            37122..37640
                     /gene="rimM"
                     /locus_tag="VW343_000684"
     CDS             37122..37640
                     /gene="rimM"
                     /locus_tag="VW343_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9755280.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            37630..38349
                     /gene="trmD"
                     /locus_tag="VW343_000685"
     CDS             37630..38349
                     /gene="trmD"
                     /locus_tag="VW343_000685"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9755281.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            38361..38699
                     /locus_tag="VW343_000686"
     CDS             38361..38699
                     /locus_tag="VW343_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755282.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(38730..39050)
                     /locus_tag="VW343_000687"
     CDS             complement(38730..39050)
                     /locus_tag="VW343_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755283.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            39148..39363
                     /locus_tag="VW343_000688"
     CDS             39148..39363
                     /locus_tag="VW343_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9755284.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(39522..39608)
                     /locus_tag="VW343_000689"
                     /pseudo
     CDS             complement(39522..39608)
                     /locus_tag="VW343_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(39659..40195)
                     /locus_tag="VW343_000690"
     CDS             complement(39659..40195)
                     /locus_tag="VW343_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9755285.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(40392..41738)
                     /gene="gor"
                     /locus_tag="VW343_000691"
     CDS             complement(40392..41738)
                     /gene="gor"
                     /locus_tag="VW343_000691"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9755286.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGLYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            42024..43223
                     /locus_tag="VW343_000692"
     CDS             42024..43223
                     /locus_tag="VW343_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9755287.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            43207..43908
                     /locus_tag="VW343_000693"
     CDS             43207..43908
                     /locus_tag="VW343_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755288.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            43910..45169
                     /locus_tag="VW343_000694"
     CDS             43910..45169
                     /locus_tag="VW343_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755289.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            45293..47290
                     /gene="metG"
                     /locus_tag="VW343_000695"
     CDS             45293..47290
                     /gene="metG"
                     /locus_tag="VW343_000695"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9755290.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
BASE COUNT        14506 a         8805 c        10289 g        13861 t
ORIGIN      
        1 actttaaaag atcggagcaa tccggtcttt ttatggagga gagtaaatga atttaaatca
       61 attagatatt atcgtttcaa atgttcccca agtctgtgct gacttggagc atatcttgga
      121 taaaaaggca gattatgctg atgatggttt tgctcagttt acgattggta gtcactgcct
      181 tatgttgtca caaaatcatt tggttccttt ggaaaacttt cagtcaggaa tcattattca
      241 tatcgagatt gaggatgtag accagaacta caaacggttg aacgagcttg gtataaaggt
      301 tttacacgga ccaactgtaa ccgattgggg aacagagtcc ttattagttc aaggacctgc
      361 tggtctagtg cttgattttt atcgtatgaa ataggtcgcg tagtcatttg acgttaacct
      421 tagctatttt taaaatagaa attgagaata tgaactattg aaagatcgga gcaatccggt
      481 ctttttatat aaagaaattg catataatac tcatctgctt tcaaaaagca ttctagtcca
      541 tctccgatta acgatggact ttatcacctc cttctccaat ccttgtatga catcttggag
      601 ttgattcatg acatcttcca aagttcgaaa ggctttattc ttaaatccac gtttacgaat
      661 ctctttccac acttgttcaa tggggttcat ctctggtgtg tatggaggaa taaatgcaaa
      721 gccaatatta gtcggaatct ttaaggtact tgatttatgc catatagcat tgtccataac
      781 gagtaaaaaa taatcatatg gataagcttg tgaaagctct tctaaaaaga cgttcatcca
      841 ctcagtatta catccaccag ctcttaagaa aaatgattcg cctgtatagg catcaacagc
      901 tccataacaa tagcgaaatt ctcgtatata gtgactatgg atatgtggac ctactcctat
      961 tggagaccaa caagatccca gtttactgat tctaccgaaa ccagcctcat cttggtacat
     1021 caagcgaacc ttacgaaaac gtctactggt tttaaatcgc tttcttttct tcttgaattg
     1081 agattttatt tttagacgcg acaatggttt gagcgtctgc tttcttagga tgttctggac
     1141 gtggcgtaat atttcgccaa ccatggcgct tcaacagttg atagaaggca tcacgtgtgt
     1201 aggaacgacc taactccttt ttataagcct gaaataaggc atcaattgta acaaattctc
     1261 ctgcctctgt agccttcaaa tgacgggcaa gaaaggcttt ctcttcctca actgtcatat
     1321 atgcatggtt acgaccacca cgtgtttctt gaaggagaga gtcgagtccg aactcctcat
     1381 attttttaca ttttgctaaa tcgttgtttg attacagtct aaaagctcta taatctcttt
     1441 ataagatttg cccatcagac gaaatataat agattgaaac tagaatagta cacctctact
     1501 tctaaaacat tgttagaaat cgatttgtcc tgttcttgtt tcattttact atagaacgat
     1561 ttgaaggcgt ttataatatt tagctgtacg agagtctttt aaaagtgttt tgatggtttg
     1621 gatttcttct ttagttgatt tcatattact attgtataat gctttttgat tttagtttag
     1681 tattatcgta gaaaatggac tcggtgctat ggatacaccg gatgagaatg gttatgtagc
     1741 agatgactat cggattactt acttagaggc ccacatcaag gccatgcgag atgccattta
     1801 ccaagatggg gttgacttgc ttggttatac gacttggagc tgtatcgatc cggtttcagc
     1861 tggcacaggg gaaatgaata agcgctatgg ctttatctat gtagatcgag ataatgtagg
     1921 aaatgggact ctcaaacgta gcaagaagaa gtccttctat tggtacatgt catttatagc
     1981 aatggtgtaa gcatcgagta gagcacattt gtttactatt tttataaaat cttctccctg
     2041 ctttataaga tgtaaaaaag agttcaaatg gaactctttt tttgctataa tgaggggaga
     2101 aaaatcagac aggagattaa gatgtcagaa ccattatttt tacaatcagt tatgcaagaa
     2161 aaaatctggg gtggtaccaa gttacgtgat gagtttggct acgacatccc aagtgaaaaa
     2221 gtcggagagt actgggctat ctcagctcat ctaaatggtg tttctaaagt tgccaatggt
     2281 cgttacgagg gaacagatct tgctactttg tatgcggaac accgtgaatt atttggcaat
     2341 cgtccagaac ctgtatttcc gcttttgacc aagatcctcg atgccaacga ctggctcagt
     2401 gtccaagttc acccagacga tgcttatgga ctcgagcatg aaggcgaact tggaaaaaca
     2461 gaatgttggt acatcattgc agcagatgaa ggttcagaga ttatctacgg tcacaatgcc
     2521 aagtcaaaag aagaactccg ccagcaaatc gaggataaga actgggatgc gctgttgaca
     2581 aaagtgccag ttaaagcagg agatttcttc tatgtaccaa gcggtaccat gcacgctatc
     2641 ggggcgggta tcttgattct tgaaacccag cagtctagcg ataccactta ccgtgtctat
     2701 gactttgacc gtaaggatga caagggcaat ttacgtgaac ttcaccttga aaaatctatc
     2761 gatgtcttga acattggtga acccgcaaat agccgtccag tgacaatcaa agcagatgat
     2821 ctacgttcca ctctccttgt atccaatgat ttcttcgcag tttacaaatg ggaaattact
     2881 ggaaaagtta actttgaaaa gacagctgac tacagcttgt tgagtgtctt ggctggtcaa
     2941 ggtcagctaa ctgttgacgg gaaaaattat ccaattcaaa aaggcagcca ctttatccta
     3001 ccaagtgatg ttgaagcttg gactctggaa gggcaaggtt tggaattgat tgttagccat
     3061 ccataaaaag aaagggcctg agttcactac tcaagtcctt tttgattaca taaattgggc
     3121 aatgaagacc acaatgatga tgattggaat gacgaaacga agaaacgaag aaggaagagc
     3181 cagacttgga acagtccttg tttccatgct ctttcatcga gatggagttc ctccattgca
     3241 agagcctttt taaagatata gcctgtaaaa agtgaaaggt agagagctcc aaatggcatg
     3301 aggagattgg aaaccaagaa gtccatagcg tcaaagaagg tcttaccaaa aatgtgaaca
     3361 tccgccatga caccgtaaga tagggctgaa ggaatgccaa agacaaaggt caaaattcct
     3421 aaaataacac tccatttggc acgtttgctg ttatcctggt tggtgatatt gcccacattg
     3481 atctccagca tcacgacaga agaagtgaca gtcgcaaaga gggagcggac gtagaaaatg
     3541 gttccaaaag gcatcttgtc aaagagttga ggcaagacga taaagagcag gctgggtccc
     3601 ccttcagact ggatattgaa gggggatcga gcttggaaaa tggctagacc tgccatgatg
     3661 gatatcgaga tattcatggc tacgatggag attcctgact ggactagatt ggttttcttg
     3721 tccaagtaag aagcataggt caacatgact gtaaccccta gtgagagggc aaagaaagat
     3781 tgtcccagag catagaggag accagcgcta gtcagttttg aaaagtctgg tttgaggaag
     3841 taaagaaccc cttccatggc atttggcaaa ctgagagagc gactgatgat aaaaacaaag
     3901 acgataaaga gcaggggcat catgactttc gaagctcttt caatcccttt ttgaacccca
     3961 cgtgatacaa tgaagatatt caataggata aaggccgctt gagctcctag ggcaatggct
     4021 ggatttgaaa tgattgaagt aaataactga gcataatcac ccgttccacc aagttggaac
     4081 aatttcccaa actcaatacc tagatagact agaatccatc ctccgataac actgtaaaaa
     4141 gataagagga taaagagggc aaaggcgcca atccaaccga taaagttgta cttgttattc
     4201 ttgcccagtt ttccaaaggt tttgatagcg gaaacgccag cactacggcc aagggcaaac
     4261 tcagccagca ggagagggaa accgattaaa atagtggaaa tgagaaagat tagtaaaaag
     4321 cctccaccgc cattagcagc agtcatgtag ggaaacttcc aaacggaacc aagcccgatg
     4381 gccagccagc agatgctaga ataaaaccaa gtttcgaccc ccattgtgat ttttctgaca
     4441 tagttaaact ctgatctcct ttttcaaaat aagaaaaagc tacttgagtt gtcaaatagc
     4501 cttctgtttg attgatagac ttgactatcc tatcatgttt tctaaggtct gtcaagaaaa
     4561 tcattttcaa gttttcacac ctttctcaaa aaagttaaaa aattttctca aaaacgcttg
     4621 actctgacct aaggggaggg gttatactat cattgtaagg aggaaatcat gtaccatata
     4681 aaagaagctg cgcagctttc aggtgtctct gtcaagaccc tgcatcacta tgacaagata
     4741 ggactcttgg tccccttaaa gtcggaaaac ggctatcgaa cctacagtca agaggatttg
     4801 gaacgccttc aggtcattct ttactacaaa tatctaggct tttctttaga gaaaatagca
     4861 gagctgttaa aggaagaaag gacagattta ttgccccatt tgactaggca gttggactat
     4921 ctaactcgcg aaaggcaaca tctggatacc ttgatttcca ccttgcaaaa aactattcaa
     4981 gaacaaaaag gagaaagaaa aatgaccatt gaggaaaaat tcacgggatt tagctatcaa
     5041 gacaatcaaa aataccacca agaagcggta gagaaatatg gtcaagaagt catgggacaa
     5101 gcgctcgaac gccaaaaagg tcacgaagac gaggctacgg ccgcctttaa ccaagtcttt
     5161 caaactttgg cacaaaatct tcaagttggt ttacctgcaa cagcaaccga aaaccaggag
     5221 caagcagcca agctcttgca agccattcgc acttatggat ttgactgctc tattgaggta
     5281 ttcggtcata tcggtaaagg ttacgtctat aacccagagt ttaaggaaaa cattgacaag
     5341 tttggttctg gaacagccca gtacacgtca gatgccattg cagcttacgt tcagacaaat
     5401 gcagaaaaaa taggctagga atttcctagc ctatttttta cttcaaatca taaagccagt
     5461 cgtcaccgtt tttgtagtaa aagaattcac tgagatcttc ttctagaaac acacgaagca
     5521 tatcagacat atcatcggtt gcaagtttta gatgagaaag attttcaaag tcctcccacc
     5581 aaactttccc ttcgtctgaa gactggagtt caccagtaaa gtgttctgtc ttgtaaaaaa
     5641 ggacgacata acgataatcc ttgtcgtcat accagttttt gataccacag agttggggtt
     5701 tggaaatgat cagaccagtt tcttctttca cttcacgaat gacagcatcg acaaaggatt
     5761 cgccacgttc aacatgacca ccaggaaaag taatgccagg ccagtcggga ctaactcggt
     5821 cttggaccag gaccttatct ccgtttttaa tcatacacat gttaacaaat tcgactgcct
     5881 ctcttctgtt cattcttcac aacctttaat ctttaatcat aatgcagact tcccgccacc
     5941 cagccggtac agagggcaga agtgatgtta aagccacccg tgtgggcatt gatatccata
     6001 acttcgcctg caaagtggag gccaggtacc agcttacttt caagggtttt aggattgatt
     6061 tccttgagac tgactccacc cttggtaaca aaggactttg caagggacat ttttccagtt
     6121 acaggaattt taagttcttt aatggactga acaagttgtt ctcgttcctt ttcagtcagc
     6181 tgtttgactt tttcaggata tccttgtaca aaaaattcgg ccaagcgttc tggtaacaag
     6241 gtttttaaag cgtttttcaa ggatttttcc cgattttctt ctagaaatgt aaccaagtcc
     6301 ttctcagaaa gttgaggcaa aacatcgagt gagagaacct ccccaccttt gacaaagcta
     6361 gacatgcgta gggcagcagg acctgacaaa ccaaagtggg taaagagtaa atcatgagtg
     6421 atgacatgct taccataact tagggtcaca tcgtccagag aaatcccttg taaggcttta
     6481 tgtggaaaat ctgttaataa aggactttca gcagcctcaa gctcggtgat ggtatgctta
     6541 aaatggcgag caatctcgtg accaaaacca gtcgaaccag tcgaaggata agacttacca
     6601 cctgttgtga caatgagttt ctcacaagtg aaggtttgat ccgctgactt aaggacaaac
     6661 tggtcatcta cttttttaac agaaacgatt tctatttgag tagcaacttg accacctagt
     6721 tcggtgattt tcttttccaa agcttcgata atagtccgag acttgtcact ggctggaaag
     6781 acgcgtccgt ggtcttcgac cttaagttta acaccatttt ctgtaaaaaa gttgatgatg
     6841 tcatgattat cgaactggga gaaaacactg taaagaaagc gtccgtttcc aggaattcca
     6901 gctagcaggt tgtctaagct accattgttg gtcacattgc aacgtccccc accagtccca
     6961 gctaattttt ttccaagttt ccgatttttt tcgatgagga gggttttctg tccataaaag
     7021 ctactggaaa tcgtagccat cataccagca ggtcccccac cgatgacaat agtatcaaaa
     7081 tgtttcatag ctctattgta ccacaaaaaa acaagagatg atggtcacct cttgtcaaga
     7141 atgcaattaa tcaatttcat agcccatcag caaaccgcca tcttctgcat agaaactgca
     7201 gagaccagag gttggtagaa ttttaatatc cgcttgtggg aaggtttcac ggattcgctc
     7261 tgagagctgt tgacaacatt tttcgttatt gcgttgggcc atgacaatac ggccaccagc
     7321 atatccagct tttactaact catcataggc agcttgaact gatttctttg atccccttgc
     7381 tttttgtagc aattcgagag tcccagtttc actagctttt ccgaccatac gaatgttgag
     7441 aaggccaacg accgtaccga taagcttgct caaacggccg ttcttcacca agttatcgac
     7501 tttggctagg acaaagagca acttagtttt ttcttgatag gcggtgatag cttcaaccac
     7561 ttcttcaaaa gacaagccct ggtcaatcaa gtcattcaat ttttctacga gtaggtcaac
     7621 ttcaccacca gcagataaac tatcaatcac atgaatctta gtgtcaggat ggtcttccag
     7681 ataaatattc tttgctagtt gagcactatt gtgactgcca gaaagggtac ctgtgatggt
     7741 tactaggaaa atgtttttgg caccttcaaa tgctcgcaaa tagtcatctg ggcttggaca
     7801 agccgatttt gaagcttctg cagttgcata catggtttcc atcatttggt caatatcgag
     7861 actggcgtca tcaacaaaga cctgatcagc tacttgaatg gttaagggga cacttacaaa
     7921 ggttgtgtta atagctggtg ttggcagttg acgataatca caaccagagt cagcaataat
     7981 tttccaagtc atagaaattc tccatctttg tcagttatac attgacaaag gttctgtctt
     8041 tttttacaat tatatcatga aaacccttga aacaaaagca tcatccgtct gttgtgacaa
     8101 gtagaaagaa aatgttatgt ctgaacgtag aatctctgaa aagtctcttg aaaatctcag
     8161 aaaatcaaac caagaatcca atttattaac cagagaagcc attgaaacag ccctcttgca
     8221 actcttggaa aaaaaggagc tgaccaagat tagtatttct gaattggtca aacgtgcagg
     8281 tgtttcgcgt gcggcctttt atcgcaatta tgactccaaa gaggaaattt tagagagcgt
     8341 ctttaaacga actgtccaca atattatgga acagctgcat cattacgatt taaagacaga
     8401 cctttatttg gtttgggttc accttttccg ggaggccaga aaggaagcta gagtgattca
     8461 attggccttg gattaccatc tggaaaaaat ctttgtccaa gccatgcagg aatttctaga
     8521 aaaataccat gggaaatcaa aaggtgtcag ctcttatctt cattccttct ggagctcagc
     8581 catcgtctct gtccttctaa aatggatcaa ggatggcatg aaggtaccag ctgaaaagat
     8641 tgcagattta ggtttaccat tttttaaaaa atagagaaaa aggagaaaag agatgactga
     8701 aaaaagactg gcctgggatg agtattttgc agcccaagcc ctactaattg cgaatcgttc
     8761 cacttgtaaa cgtgctaaag tgggcgcgat tctggtaaaa gataataagg ttatttccac
     8821 tggttacaat ggttcggtgt cagggactga gcattgtatt gatcacgaat gtttggtcat
     8881 tgaaggccac tgtgttcgca cccttcacgc tgaggtcaat gctatccttc aaggtgctga
     8941 gcgtggtgtt cccaaaggct ttacagccta tgtaacccat tttccttgtc tcaactgtac
     9001 aaaacaattg cttcaggtcg gctgcaagcg cgtggtttat atcaaccagt accgaatgga
     9061 cgactacgcc caataccttt atcaagaaaa agggacagaa ttgacccatt taccacttga
     9121 gacagtacag acagctctta aagaggctga tctaatgtaa aaattatcaa aaataaatgg
     9181 cttagaaaga tttttaaacc gttttttggt ataataagaa gaataaattg aaagaaggaa
     9241 ttccaaaaat gggaaaaatt gaagttatta atcacccact gattcaacac aaattgtcaa
     9301 tcttgcgtcg tacagatact tctacaaaag cttttcgtga gctagtagat gagattgcaa
     9361 tgttgatggg gtatgaagta cttcgtgatc ttccactaga agatgtggaa atcgaaacac
     9421 caattacaaa aacagttcaa aaacaattgg caggtaagaa attggccatc gtcccaatct
     9481 tgcgtgcagg tatcgggatg gttgatggtc tcttgagctt ggttccagct gctaaagttg
     9541 gccacatcgg tatgtaccgt gatgaagaaa cacttcaacc agttgaatac ttggtgaaat
     9601 tgcctgagga cattgaccaa cgtcaaattt ttatagtaga cccaatgttg gcaacaggtg
     9661 gctcagcaat cttggctgtt gattctctta aaaaacgtgg cgcatcaaat atcaaatttg
     9721 tctgccttgt atctgctcca gagggtgtaa aagcccttca agaagctcat ccagatgtag
     9781 aaatctttac agcagccttg gatgaacgtt tgaacgaaca cggttatatc gttccaggtc
     9841 ttggagatgc tggagaccgc ttgttcggta caaaataaga tcgaaaagaa gaatgacttg
     9901 gaaaagtatt tccagtcacg aaaggaggtt gggtttttgt ttctgtctaa tgaaagcaga
     9961 gcaaaaattt gacctttttt gaccaagata ttataatagt cttatcttca gtcagttgac
    10021 caacaaaatt aaaactcaaa aggagaaatg aatgattcct gtagttattg aacaaacaag
    10081 ccgtggagaa cgttcttacg atatttactc acgtcttctc aaagaccgca tcattatgct
    10141 gacaggtccg gttgaagaca atatggctaa ctctgttatt gcccaattgc ttttcttgga
    10201 tgcccaagat agtacaaaag atatttacct ttatgtcaat acaccaggtg gttctgtttc
    10261 agctggtttg gcaatcgtag ataccatgaa ctttatcaag gcagatgtcc aaaccatcgt
    10321 tatgggaatg gctgcatcta tgggaacagt catcgcatca agtggagcaa aaggcaaacg
    10381 tttcatgctt ccaaatgctg aatacatgat tcaccaacca atgggcggta caggtggtgg
    10441 tacccaacaa actgatatgg ctatcgctgc agaacacttg ctcaaaactc gtaatacctt
    10501 ggaaaaaatc ttggctgaaa attcaggtca gtcaatggaa aaagtccatg cagatgcaga
    10561 acgtgataac tggatgagcg cccaggaaac acttgaatat ggctttattg atgaaattat
    10621 ggccaacaat tcattgaact aatgatgata gaaggcaaac tcgactgggt ttgctttttt
    10681 tggtataata gggagagatt tcttagaaag aggatttatc atgtttgaaa aagtcaatcg
    10741 ctctggcttg attatctatc tttactataa tcgtgatgcc aaaaaattgc aggattatgg
    10801 agatattacc tatcattcca agaaacatcg ttacttacaa ctctatgttc caactcaaga
    10861 agtggagcaa ttggtcggac gcttgagcaa ggaaaaattt attaaaaaag ttagggtttg
    10921 tcatatccaa gagttggaaa caccctttgt gggcaatctt tatcgatagg aaaacgttat
    10981 catagaaaaa gttcaaggaa aacattgaca attttctgat aattcggtat attcttaaca
    11041 gactatttaa gaaataagga gacaaaaaga tgaagaaaaa atttgcccta tcgtttgtgg
    11101 cgcttgcaag tgtagcactt cttgcagcct gtggagaagt gaagtctgga gcagtcaaca
    11161 ctgctggtaa ctcagtagag gaaaagacaa ttaaaatcgg gtttaacttt gaagaatcag
    11221 gttctttagc tgcatacgga acagctgaac aaaaaggtgc ccaattggct gttgatgaaa
    11281 tcaatgccgc aggtggtatc gatggaaaac aaatcgaagt aatcgataaa gataataagt
    11341 ctgaaacagc tgaggctgct tcagttacaa ctaaccttgt aacccaatct aaagtatcag
    11401 cagtcgtagg acctgcgaca tctggtgcga ctgcagctgc ggtagcgaac gctacaaaag
    11461 caggtgttcc attgatctca ccaagtgcga ctcaagatgg attgactaaa ggtcaagatt
    11521 acctctttat tggaactttc caagatagct tccaaggaaa aattatctca aactatgttt
    11581 ctgaaaaatt aaatgctaag aaagttgttc tttacactga caatgccagt gactatgcta
    11641 aagggattgc caaatctttc cgcgagtcat acaagggtga aatcgttgca gatgaaactt
    11701 tcgtagcagg tgacacagac ttccaagcag cccttacaaa aatgaaaggg agagactttg
    11761 atgctatcgt tgttcctggt tactataatg aggctggtaa gattgtaaac caagcgcgtg
    11821 gcatgggaat tgacaaacca atcgttggtg gtgatggatt caacggtgag gagtttgtac
    11881 aacaagcaac tgctgaaaaa gcatcaaaca tctactttat ctcaggcttc tcaactactg
    11941 tagaagtttc agctaaagct aaagccttcc ttgacgctta ccgtgctaag tacaatgaag
    12001 agccttcaac atttgcagcc ttggcttatg attcagttca ccttgtagca aacgcagcaa
    12061 aaggtgctaa aaattcaggt gaaatcaaga ataaccttgc taaaacaaaa gattttgaag
    12121 gtgtaactgg tcaaacaagc ttcgatgcag accacaacac agtcaaaact gcttacatga
    12181 tgaccatgaa caatggtaaa gttgaagcag cagaagttgt aaaaccataa tagaaaaatg
    12241 ttgaaatagg gaatgagcct ttgactcact ccctgtttcg atatttaata ctcttcgaaa
    12301 atctcttcaa actgcgtcaa cgtcgccttg gattatatat gtgactgact tcgtcagtct
    12361 tatctacaac ctcaaagcag tgctttgagc aacctgcggc tagtttccta gtttgctctt
    12421 tgattttcat tgagtataag aacctatcaa aaagtgaggg aaaaccctcg gaattataaa
    12481 tagaaagagt gaatcttatg ctccaacaac tcgtaaatgg tttgattcta ggtagtgttt
    12541 acgcgctgtt agccctagga tataccatgg tttacggaat tatcaagctc atcaacttcg
    12601 cccatggtga tatttatatg atgggagcct ttatcggtta tttcttgatc aattctttcc
    12661 aaatgaattt ctttgtagcg cttattgtag ctatgctagc gacagctatt cttggtgtcg
    12721 tgattgagtt tcttgcttac cgacctttgc gccactctac tcgtattgct gttttgatta
    12781 cggctattgg ggtttctttc ctattggagt atggaatggt ctatctggtt ggtgccaata
    12841 cccgtgcctt ccctcaagcg attcaaacag ttcgatatga tttgggagca attagcttaa
    12901 caaatgtgca gttaatgatt ttggccattt ccttgatttt gatgattttg ttacaagtca
    12961 ttgtccaaaa gactaagatg gggaaagcca tgcgtgcagt atcagtagat agcgacgcgg
    13021 cacaattgat ggggatcaat gtaaaccgta cgattagctt taccttcgct ttgggttctg
    13081 ctcttgcggg tgcggctggt gttctgattg ctctttatta taactctctt gagcctttga
    13141 tgggggttac tccaggtctt aaatctttcg ttgccgcagt acttggtggt atcggaatta
    13201 ttcccggtgc ggctcttggt ggctttgtga ttggtctatt ggaaaccttt gcgacagcct
    13261 ttgagatgtc agatttccgt gatgccattg tttatggaat cttgttgttg atcttgattg
    13321 tccgcccagc tggtatcctt ggtaagaatg tgaaagagaa ggtgtaaacg atgaaggaaa
    13381 atttaaaagt taatattcta tggttactcc ttttgttagc tggctatagc ttgattagtg
    13441 tactggtttc agtcggagta cttaatctat tctatgtaca gattttacaa caaattggaa
    13501 ttaatattat tttggctgtt ggtctcaact taatcgttgg tttttcagga caattttcac
    13561 ttggtcatgc tggtttcatg gcgattggtg cctatgcagc agctattatt ggttctaaat
    13621 caccaaccta cggtgccttc tttggagcta tgcttgtagg ggctttgctt tcaggagcag
    13681 ttgccttact tgtcggcatt ccaaccttgc gcttgaaggg ggactatctt gcggtagcaa
    13741 ctctgggtgt ttctgaaatt atccgtatct ttatcatcaa tggtggaagt cttacaaatg
    13801 gtgcggcagg tatcttaggg attcctaact ttacaacttg gcaaatggtt tacttctttg
    13861 tcgtgattac aaccattgca accttgaact tcttgcgtag cccaattggc cgttcaaccc
    13921 tctctgttcg tgaagatgaa atcgctgctg agtcagttgg ggttaatacg actaaaatta
    13981 aaatcatcgc ttttgtcttt ggtgccatta ctgcaagtat tgctgggtca cttcaggcag
    14041 gatttatcgg gtctgttgta ccgaaagatt acaccttcat caactcaatc aacgttttga
    14101 ttattgttgt atttggtgga ctcggttcca ttacaggtgc gattgtttcg gctattgttc
    14161 tgggaatttt gaatatgctt ctccaagatg ttgctagtgt gcgtatgatt atttacgctt
    14221 tggccttggt attggtaatg attttcagac caggtggact ccttggaacg tgggaactga
    14281 gcctatcacg tttctttaaa aaatctaaga aggaggaaca aaactaatgg cattacttga
    14341 agtaaaacag ttaaccaaac attttggtgg tctaacagct gttggagatg tgactcttga
    14401 attgaacgaa ggggaactgg ttggattaat cggtccaaac ggagctggga aaaccaccct
    14461 tttcaacctt ttgaccggtg tttatgaacc aagcgaggga acagtaaccc tagatggtca
    14521 ccttttgaat gggaaatcac cttataagat tgcctctttg ggacttggac gtactttcca
    14581 aaatatccgt ctctttaaag atttaacagt tttagataat gttttgattg cttttggaaa
    14641 ccatcacaaa cagcatgttt ttactagttt cttacgctta ccagcttttt acaagagtga
    14701 aaaagaatta aaggataaag ctttggaatt gttgaaaatc tttgatttag atggtgatgc
    14761 agagactctt gctaaaaatc tttcctacgg acaacaacgt cgtttggaaa ttgttcgtgc
    14821 ccttgctacg gaacctaaaa ttctcttctt agatgaacca gcagcaggta tgaacccaca
    14881 ggaaacagcc gaattgactg agttaattcg tcgtatcaaa gatgaattta agattacaat
    14941 catgttgatt gaacacgata tgaatctggt catggaagta acagaacgta tctacgtact
    15001 tgaatatggc cgtttaattg ctcaaggaac tccagacgaa attaagacca ataaacgcgt
    15061 tatcgaagct tatctaggag gtgaagccta atgtctatgt taaaagttga aaatctttct
    15121 gtgcattacg gtatgatcca agcagtccgt gatgtaagct ttgaagttaa tgaaggagaa
    15181 gttgtttccc ttatcggtgc caacggtgca ggtaagacaa ctattcttcg caccttgtca
    15241 ggtttggttc gaccaagttc aggaaagatt gaatttttag gtcaagaaat ccaaaaaatg
    15301 ccagctcaga aaatcgtggc aggtggtctt tcacaagttc cagaaggacg ccacgtcttt
    15361 cctggcttga ctgttatgga aaatcttgaa atgggagctt tcttaaagaa aaatcgtgaa
    15421 gaaaatcaag ctaacttgaa gaaggttttc tcacgctttc ctcgtcttga agaacggaag
    15481 aaccaagatg cagctactct ttcaggaggg gaacaacaaa tgcttgccat gggacgcgct
    15541 cttatgtcaa caccaaaact tcttctttta gatgaaccat caatgggact tgccccaatc
    15601 ttcatccaag agatttttga tatcattcaa gatattcaga agcaaggaac aaccgtcctc
    15661 ttgattgaac aaaatgccaa taaagcactt gcaatctctg accgaggata tgtattggaa
    15721 acaggaaaaa tcgtcctatc aggaacagga aaagaactcg cttcatcaga agaagtcaga
    15781 aaagcatatc taggtggcta aaacaatcca gtggattgtt ttagtcggca gatgaagatt
    15841 acgaagtaat catcaatata gtccgggggc cctttttagt cggtggatga agattgcgaa
    15901 gcaatcatca aatccagagg attgttttag ccggtagatt gagattgcaa acaaatctgc
    15961 atctacattg aaagctgaat ttctaataat tgaaaaaatc gaatgaaacg tatcttacct
    16021 tcattcaggg agctcgattt tagagctctt tttgctagct tattcatact tttttgaatt
    16081 tcgaaaaaga aatgtaagcg tttgatagat ttacaaaaag attgtataat ggggataaga
    16141 atagaaaagg agaagtctca tggcagttaa agattttatg acccgcaagg tagtttatat
    16201 tagtccagat acaacagtat ctcatgcagc agatttgatg agagagcaag aattgcaccg
    16261 cttgcctgtt atcgaaaatg atcaattggt tggtttggtg actgagggaa ccattgcaca
    16321 agcaagtcca tctaaagcaa caagtctttc tatctatgag atgaattatc ttctgaataa
    16381 gacaaaagta aaagatgtca tgattcgcga tgttgtcact gtctcaggct atgctagtct
    16441 agaagatgca acttatctga tgttgaaaaa taagattggt attctccctg tcgtagataa
    16501 ccatcaagta tacggagtta ttactgaccg tgacgttttc caagcctttc ttgaaattgc
    16561 aggttatggc gaagaaggga ttcgtgtacg ctttgttaca gaagatgaag ttggtgttct
    16621 tggaaaaatt gtttctttga ttgtagaaga aaatttgaat atctcccata cagtcaatat
    16681 tccgcgtaag gatggtaagg tcattatcga agtgcaaatc gatggatcaa ttgatttacc
    16741 agccttgaaa gaaaaatttg aagcaaatgg tattcaagtg gaagaaatcg ctcgcacttc
    16801 agcaaaagtc ttgtaagaag ggaagcccaa aggcttcttt tttcatgaaa aggggattag
    16861 agaaaaagat ggaaagaaat gataaaatat gctataatga aataatgtaa aaaaggagta
    16921 tttatggaca tttcagtaat tcgtcagaaa attgacgcaa atcgtgaaaa attagcttct
    16981 ttcagggggt ctctttgacc tcgaagggct agaggaagag attgccatct tggaaaacaa
    17041 gatgacagaa cctgattttt ggaacgataa tattgcggcc caaaaaacgt cgcaagaatt
    17101 aaatgaatta aaaaacactt acaatacctt ccataagatg gaagagttgc aggatgaagt
    17161 cgaaatttta ttggattttt tggctgaaga cgagtcagtg catgatgaac tggtagcgca
    17221 gttagccgaa cttgataaga taatgaccag ctacgagatg actctactct tgtcagaacc
    17281 ttatgaccac aacaatgcca tcttggaaat ccatccaggt tctggtggta ctgaggcgca
    17341 ggactggggt gatatgttgc ttcgtatgta tactcgttat ggtaatgcta aaggctttaa
    17401 agtggaagtg ttggattacc aagcaggtga tgaggctggt attaagtcgg taactttatc
    17461 atttgaaggg cctaatgcct atggtctcct caagtcagaa atgggtgttc accgcttagt
    17521 gcgaatctca ccatttgact ctgccaaacg tcgccatacc tctttcacat ctgtagaagt
    17581 gatgccagaa ttggatgata ctattgaagt ggaaatccgt gaagatgata tcaagatgga
    17641 taccttccgt tcaggtggtg ccggtggaca aaacgtcaat aaggtttcaa caggtgtacg
    17701 tttaacccac attccaactg gaattgttgt ccaatcaaca gtggatcgta cccagtatgg
    17761 aaatagagat cgtgccatga agatgttgca ggctaagctc tatcaaatgg agcaagataa
    17821 gaaggctgcg gaggtagatt ctctcaaagg tgagaaaaag gagatcactt ggggaagcca
    17881 aatccgttct tatgtcttca cgccttatac tatggtaaaa gatcaccgaa ctagctttga
    17941 ggttgctcag gtagataagg ttatggatgg ggacctagat ggttttatcg atgcttatct
    18001 caagtggcga attagctaag atagaaagga actcacatgt caattattga aatgagagat
    18061 gtcgttaaaa aatacgacaa cggaacaact gctctacgcg gtgtttcggt tagcgttcaa
    18121 ccgggggaat ttgcttacat cgtaggacct tcaggagcag ggaagtcaac ttttattcgt
    18181 tctctgtatc gtgaagtaaa aatcgataaa ggaagcctat cagttgctgg ttttaatctg
    18241 gttaagatca aaaagaaaga tgtcccgctt ctacgtcgta gtgttggggt tgtcttccag
    18301 gattataaac tgttaccaaa gaaaactgtc tatgaaaata ttgcttacgc tatggaagta
    18361 atcggggaaa atcgccgtaa tatcaaaaga cgagtgatgg aagttttgga cttggttgga
    18421 ttgaagcata aggttcgttc ttttccaaat gaactctcag gtggggagca acagcgaatt
    18481 gcgattgcgc gtgcaattgt aaataatccc aaagtattga tagctgatga gccaacagga
    18541 aatctggatc cggataattc atgggaaatt atgaatctct tggaacggat taacctacaa
    18601 ggaacaacta ttttgatggc gactcataat agccagattg taaatacctt gcgccaccgt
    18661 gtcattgcca ttgaaaatgg ccgtgtcgtt cgtgacgaat caaaaggaga gtatggatac
    18721 gatgattagt agattttttc gccatttatt tgaagcctta aaaagtttga aacgaaatgg
    18781 ttggatgaca gtagctgctg tcagttcagt catgattact ttgaccttgg tggcaatatt
    18841 tgcatctgtt attttcaata cagcgaaact agctacagat attgaaaata atgtccgtgt
    18901 agtagtttat atccgaaagg atgtggaaga taatagtcag acaattgaaa aagaaggtca
    18961 aactgttaca aataatgact actacaaggt atatgattct ttgaagaaca tgtctacggt
    19021 taaaagtgtt accttttcaa gtaaagaaga acaatatgaa aaattaaccg agataatggg
    19081 agataactgg aaaatctttg aaggagatgc caatcctctc tatgatgcct atattgtaga
    19141 ggcaaacact ccaaatgatg taaaaactat agccgaagat gctaaaaaaa ttgaaggtgt
    19201 ctctgaggtt caagatggcg gtgccaatac agaaagactc ttcaagttag cttcatttat
    19261 ccgtgtttgg ggactaggga ttgctgcttt gttaattttt atcgcagttt ttttgatttc
    19321 aaataccatt cgtattacca ttatttcccg cagtcgcgaa attcaaatca tgcgcttggt
    19381 cggagctaaa aacagttata tccgtggacc gttcttgtta gaaggagcct ttatcggttt
    19441 attgggagct atcgcaccat ctgttttggt ctttattgtt tatcaaattg tttatcaatc
    19501 tgtcaacaaa tcgttggtag ggcaaaatct atccatgatt agtccagatt tatttagtcc
    19561 gttgatgatt gccctactat ttgtgattgg ggttttcatt ggttcattgg gatcaggaat
    19621 atccatgcgc cgattcttga agatttaggt aaaatagctg cttttatgag gagattgtaa
    19681 aatctccttt tttgctacaa gagtttttga aaagagatgc gcagaagaaa agagcttcca
    19741 aagaagtccc ccagagaaga cttcttgatg gctaatttga acttgaaatt tgactcacta
    19801 gaaatttttt agaaaaaagt gtaatttttt tataaaagcc tttacaaaaa aaattaaagt
    19861 ggtataatta aattatagaa ggtgcaaacg ttttcgtaaa acgtttgcct aaataaaaat
    19921 aaaggagatt tgaatgatga aagatacatt caaaaatgtc ttgtctttcg aattttggca
    19981 aaaattcggt aaggctttga tggtagttat cgcggttatg ccggctgctg gtttgatgat
    20041 ttcaatcggt aagtctatcg tgatgattaa cccaaccttt acaccacttg tcatcacagg
    20101 tggaattctt gagcaaatcg gttggggggt tatcggtaac cttcacattt tgtttgccct
    20161 agccattgga ggaagctggg ctaaagaacg tgctggtggt gctttcgccg ctggtcttgc
    20221 cttcattttg attaaccgta tcactggtac aatttttggt gtatcaggcg atatgttgaa
    20281 aaatccagat gctatggtaa ctactttctt tggtggttca atcaaagttg ctgattactt
    20341 tatcagtgtt cttgaagctc cagccttgaa catgggggta ttcgtaggga ttatctcagg
    20401 ttttgtaggg gcaactgctt acaacaaata ctacaacttc cgtaaacttc ctgatgcact
    20461 ttcattcttc aacgggaaac gtttcgtacc atttgtagtt attcttcgtt cagcaatcgc
    20521 tgcaattcta cttgctgctt tctggccagt agttcaaaca ggtatcaata acttcggtat
    20581 ctggattgcc aactcacaag aaactgctcc aattcttgca ccattcttgt atggtacttt
    20641 ggaacgtttg ctcttgccat ttggtcttca ccacatgttg actatcccaa tgaactacac
    20701 agctcttggt ggtacttatg acattttaac tggtgcagct aaaggtactc aagtattcgg
    20761 tcaagaccca ctatggcttg catgggtaac agaccttgta aaccttaaag gtactgatgc
    20821 tagtcaatat caacacttgt tagatacagt acatccagct cgtttcaaag ttggacaaat
    20881 gatcggttca ttcggtatct tgatgggtgt gattgttgct atctaccgta atgttgatgc
    20941 tgacaagaaa cataaataca aaggtatgat gattgcaaca gctcttgcaa cattcttgac
    21001 aggggttact gaaccaatcg aatacatgtt catgttcatc gcaacaccta tgtatcttgt
    21061 ttactcactt gttcaaggtg ctgccttcgc tatggctgac gtcgtaaacc tacgtatgca
    21121 ctcattcggt tcaatcgagt tcttgactcg tacacctatt gcaatcagtg ctggtattgg
    21181 tatggatatc gttaacttcg tttgggtaac tgttctcttt gctgtaatca tgtactttat
    21241 cgcaaacttc atgattcaaa aattcaacta cgcaactcca gggcgcaacg gaaactacga
    21301 aactgctgaa ggttcagaag aaaccagcag cgaagtgaaa gttgcagcag gctctcaagc
    21361 tgtaaacatt atcaaccttc ttggtggacg tgtaaacatc gttgatgttg atgcatgtat
    21421 gactcgtctt cgtgtaactg ttaaagatgc agataaagta ggaaatgcag agcaatggaa
    21481 agcagaagga gctatgggtc ttgtgatgaa aggacaaggg gttcaagcta tctacggtcc
    21541 aaaagctgac attttgaaat ctgatatcca agatatcctt gattcaggtg aaatcattcc
    21601 tgaaactctt ccaagccaaa tgactgaagc acaacaaaac actgttcact tcaaagatct
    21661 tactgaggaa gtttactcag tagcagacgg tcaagttgtt ggtttggaac aagtaaagga
    21721 tccagtattt gctcaaaaaa tgatgggtga tggatttgca gtagaacctg caaatggaaa
    21781 cattgtatct ccagtttcag gtactgtgtc aagcatcttc ccaacaaaac atgcttttgg
    21841 tattgtgacg gaagcaggtc ttgaagtatt ggttcacatt ggtttggaca cagtaagtct
    21901 tgaaggtaaa ccatttacag ttcatgttgc tgaaggacaa aaagttgcag caggagatct
    21961 ccttgtcaca gctgacttgg atgctatccg tgcagcagga cgtgaaactt caacagtagt
    22021 tgtcttcaca aatggtgatg caattaaatc agttaaatta gaaaaaacag gttctcttgc
    22081 agctaaaaca gcagttgcta aagtagaatt gtaatatact tgaggttgga agctgtattc
    22141 caacctctta ttttgggaga aaagaatgaa atttttaaca ctcaatactc acagttggat
    22201 ggagaaagaa gcagaggaaa aattccagat tttgcttgaa gatattcttg aaaaggacta
    22261 tgatttgatt tgtttccaag aaatcaatca ggagatgacc tcgtcagagg tggaggttaa
    22321 tgacctttat caagctttgc cagcagctga gcctattcac caagaccatt atgttagact
    22381 cttggttgaa aagttgttag agcaagggaa aaattactac tggacctggg cttataacca
    22441 tatcggctat aaccgctacc acgaaggtgt ggctatcttg tctaaaacac ctattgaagc
    22501 cagagaaatt ttggtttcag atgtggatga tccaacagac tatcatactc gccgtgttgc
    22561 cctagctgaa actgtagtcg atggcaagga gctagcagtt gccagtgttc atctctcttg
    22621 gtgggataaa ggtttccaag aagaatgggc acgatttgag gctgtcttga aaaaattgaa
    22681 caagccactt ttactagctg gagattttaa caacccagca ggtcaggaag gttaccaagc
    22741 tattttagct agtccattag gcttacaaga cgcatttgaa gttgctcaag agaaaagtgg
    22801 tagctatact gttccgcctg aaattgatgg ctggaaaggg aacactgaac cccttcgaat
    22861 cgattatgtc tttactacca aagagttagc ggtggaaaat ttacatgtcg tatttgatgg
    22921 taacaagagt ccacaagtga gtgatcacta tggcttgaat gctatgttaa actggaaata
    22981 ataactgaaa agaggttgga actataaaat tccagccttt tcttactaga gaagctactg
    23041 gaaatagcct aaataagtga gactactgta atggaataaa atatggtata attgataaga
    23101 tagatagaat cgaggatgtt atgtcattta cgaaatttca atttaaaaac tatattagag
    23161 aagccttgga ggagttaaaa tttacaactc caacagaggt gcaagacaag ttgattccta
    23221 ttgttttggc aggtcgtgac ctagtaggag aatcaaaaac aggttcaggt aagactcata
    23281 ctttcttgtt accgattttc cagcaattag atgaagctag cgatagtgta caagcagtga
    23341 ttactgcacc gagtcgtgag ttggctactc aaatttacca agtagcgcgt cagatttcag
    23401 ctcactcaga tgtcgaagtt cgtgtggtta attatgtggg tggtacggat aaggctcgcc
    23461 agattgagaa attggaaagc aatcagcctc atattgttat tggaacacca ggccgtatct
    23521 acgacttggt taaatctggt gatttagcta ttcataaagc caagacattt gttgttgatg
    23581 aagcagatat gaccttggat atgggattct tggaaattgt tgataagatt gctggcagtc
    23641 ttccaaaaga cttgcaattc atggtcttct cagcgactat cccacaaaaa ctgcaaccat
    23701 tcttgaaaaa atacttatca aatcctgtta tggagaaaat taagactaaa acggttattt
    23761 ctgacaccat tgataattgg ttgatttcga ccaagggaca tgataggaat gctcaaattt
    23821 accaattgac tcagttgatg cagccgtatt tggcaatgat ttttgttaac actaaaacgc
    23881 gtgctgatga attgcattca tatctgactg ctcaaggctt gaaggttgca aaaatccatg
    23941 gagatattgc ccctcgtgaa cgcaagcgaa tcatgaatca ggtgcaaaat ctggattttg
    24001 agtatattgt cgcaacagat ttagcagcgc gtgggattga cattgaaggt gtcagccatg
    24061 tcatcaatga tgccattccg caagacttat ctttttttgt tcaccgtgtt ggtcgtactg
    24121 gacgaaatgg cctaccaggt acagctatta ccctttatca gccaagtgat gactcggata
    24181 tccgtgagtt ggagaaattg ggaatcaagt ttagtcctaa gatggtcaaa gatggggaat
    24241 ttcaagatac ctatgaccgt gatcgtcgtg ccaaccgtga gaaaaaacaa gataaacttg
    24301 atatcgaaat gattggtttg gttaaaaaga aaaagaaaaa agtcaaaccg ggttataaga
    24361 agaaaattca atgggcggtt gatgaaaagc gccgtaaaac taagcgtgct gaaaatcgcg
    24421 ctcgcggtcg tgcagagcgt aaagctaaac gccaaacatt ttaatagaaa ttgttggagt
    24481 actgagctcc aactttttta tttatgagaa cgaactatct aaaccgaaac actacattaa
    24541 agactgcaaa ttgcgattaa aaatggtata atgataaagt tatatagtcc cgataagatg
    24601 gtaggtattt attacgaaga gttttcctat cagtagtttg caattcgcaa agaatgacgg
    24661 cttcgaggtt gctcgtcttt ctacggttgc cgccatactg cggtcaccct atgcgcctta
    24721 ctagcttcag aaataaaaaa gtatcgtttt tatttctgaa gctagtaact cttagataat
    24781 ggaaattcct atcagtactt tgtaactcta taacaatatt ttttaagggg ggacattttt
    24841 atgtcagaac gtaaattatt cacgtctgaa tctgtatctg aggggcatcc ggataagatt
    24901 gcagaccaaa tttcagatgc gattttggat gctattttag caaaggatcc agaggcgcac
    24961 gttgctgctg aaacagctgt atatactggt tctgtccacg tttttggtga aatttctaca
    25021 aatgcctatg tggatattaa ccgtgtggtt cgtgatacca ttgcagagat tggttatacc
    25081 aatacagaat atggattttc tgctgagacg gtgggagtac acccatcttt ggtggaacaa
    25141 tctcctgaca tcgctcaagg tgttaacgaa gccttggagg ttcgtggaaa tgctgatcaa
    25201 gatccactgg acttgattgg agcaggtgac caagggctca tgtttggatt tgcagtagat
    25261 gaaacagaag agctcatgcc attgccaatt gcactcagtc ataaattggt tcgtcgtctg
    25321 gcagaacttc gtaagtctgg agaaattagc tatctccgtc cagatgcaaa atcacaagtt
    25381 acagttgagt acgatgaaaa tgaccgtccg gtacgtgtag atacagtcgt tatttctact
    25441 cagcatgatc cagaggccac taatgaacaa atccatcaag atgtgattaa caaggtcatc
    25501 aaagaagtta ttccatcttc ttatcttgat gataagacaa aattctttat caatccgaca
    25561 ggtcgttttg taatcggtgg tcctcaaggg gactcaggtt tgactggtcg taagattatt
    25621 gtagatactt atggtggcta ctctcgtcat ggtggtggtg ccttctctgg taaagatgcg
    25681 actaaggtgg atcgttcagc ctcttatgcg gctcgctata ttgccaagaa tatcgttgca
    25741 gcaggccttg ctaagaaggc agaagtgcag ttggcctatg ctatcggtgt tgcgcaacct
    25801 gtttctgttc gtatcgatac tttcggtaca ggaacagtag ctgaaagtca acttgaaaaa
    25861 gcggctcgtc aaatctttga ccttcgccct gcagggatta tccaaatgct ggacctcaag
    25921 cgtccaattt accgtcaaac atcggcttac ggtcacatgg gacgtacaga tattgatctt
    25981 ccatgggaac gtttggataa ggtagatgct ttgaaagaag cagtaaaata agattttaag
    26041 aggggaacgt cctctctttt ttatagtttt taactatact gggatactgt tctgaaaatc
    26101 cattttgcga aagtagagat ttacatgtat agtagattga aactagaata gtacacctca
    26161 acttctaaaa cattgttaga aatcgatttg actgtcctga tcgatttctc ctgttcttgt
    26221 ttcattttac tatatttctt taaaaatgat aaaggttgag atttctcctc gtaatagata
    26281 atcatgggga tatttcaatc caaagtttta ttcgttatca cttgactatt gcaaggtttt
    26341 ctagagcaac agagtcatgg aatggactca tggttgagat ttctccttgt tgcttggact
    26401 tcattcaaaa gtctgttacc caagccttgt tcaaacttcc aatacactag ctgtttccat
    26461 agcatgactt ctgtactaga ctttcttttc cgaataaata gatagaacca cagaatctag
    26521 taaacctaga attaaaatta tggtataata ttagcaataa aagaaatctg gaggattaga
    26581 atcatggtat caacgaaaac acaaattgct ggttttgagt ttgacaattg cttgatgaat
    26641 gcagcaggtg tggcttgtat gacgatagag gagttagaag aggtcaaaaa ctcagcggca
    26701 ggaacctttg ttactaagac agcgaccttg gacttccgtc aggggaatcc tgagccacgc
    26761 taccaagatg ttccacttgg ttccatcaac tctatgggct tgccaaataa tggcttagac
    26821 tattatttgg attatctttt agatttgcag gaaaaagagt cgaaccgaac tttcttctta
    26881 tctctggtcg gcatgtctcc agaggaaacc catactattt tgaaaaaagt ccaagagagt
    26941 gattttcgtg gtctgactga gctaaatctt tcctgtccaa atgttccagg taaacctcag
    27001 attgcctatg attttgagac aacagaccgg attttggcag aagtgtttgc ttacttcacc
    27061 aaacctcttg gaattaaatt gccaccttat tttgatattg ttcactttga ccaagcggca
    27121 gctattttca acaaatatcc gctcaagttt gtcaactgcg ttaactctat cggaaacggc
    27181 ctctatatag aagacgaatc tgtcgttatt cggcctaaga atggttttgg tggaattggt
    27241 ggagaataca tcaaaccgac tgctctagcc aatgttcacg ccttttatca acgtttaaat
    27301 cctcaaatcc aaattatcgg aacaggtggc gttctgactg gtcgagatgc ctttgaacac
    27361 atcctctgtg gagcaagtat ggtgcaggtg ggaacgaccc ttcacaaaga aggcgtcagt
    27421 gcttttgacc gcattaccaa tgaactgaaa gcaatcatgg tggaaaaagg ctacgagagc
    27481 ttagaagatt tccgtgggaa attgcgctat attgactaaa ttaaatcgaa aaatcagaag
    27541 aaagaaggga cgatgctagc cattgaagaa agtcagaagt tgactttatc aaatttaccg
    27601 agcctgagcc tatttacagg gacagatcag ggtcagtttg aagtgatgaa gagtcaaatg
    27661 ttgaaacaga ttgggtatga ttctgctgac ctcaactttg cctactttga tatgaaagaa
    27721 gtagtttaca aggatgtgga actggagttg gtcagccttc ctttctttgc ggatgagaaa
    27781 atcgtgatat tagattattt tatggatatc acgactgcta agaaacgctt tttgacagat
    27841 gatgagctta agtcatttga ggaatacctt gacaatcctt ctccaacaac caagttgata
    27901 atctttgcag aaggaaagct ggatagcaaa agacggttag tcaaattact taagcgtgat
    27961 gccaaggtct tcgatgcagt agaagtaaaa gaacaagaat tgcgccagta tttccaaaag
    28021 tggagtcaga aacaaggtct gcagtttacc aatcattctt ttgaaaatct cctcatcaag
    28081 tcggggtttc aatttagcga aatccagaaa aatcttctct ttttacagtc ctataaggcg
    28141 aattctgtta tcgaggaaga ggatattgtt aacgcaattc ccaagacctt gcaggacaat
    28201 atttttgatt taactcagtt tattctgact aaaaagatgg atcaggcgcg cgatttagtg
    28261 agagacttga ccttgcaagg ggaagatgaa atcaaactga ttgcagtcat gctgggacaa
    28321 tttcggactt ttactcaggt gaagattttg gcggagtctg gccaaacaga atcgcagatt
    28381 gcaagtagtt taggtagtta tctgggacgt aacccaaatc cttatcaaat caagtttgca
    28441 ttaagagatt cgagaggact ttctttgagc tttctgaagc aagctatttc ctatttgatt
    28501 gagacagact atcagattaa gacaggtctt tatgaaaaag gtttcctttt tgaaaaggca
    28561 ctcttacaga ttgctagtca ggtcaattga catttgttga aactacaacc cgcgtcagga
    28621 ttatcttgtc gtgggtttct tgctgatttg cttattttat tagaaagtat atcagtgatt
    28681 tccttatcta taagcaaaaa acttgaaaaa agtgaatgtt tgcgttatca ttttcatata
    28741 gaaagaaaaa gaggtattac agatggctat tatcttacca gaacttccat atgcatacga
    28801 cgctttggaa ccatacatcg atgcggaaac aatgcatttg caccatgaca aacaccatca
    28861 aacttatgtc aacaatgcca atgcagcttt agaaaaacac cctgaaatcg gtgaagacct
    28921 tgaagccttg cttgctgatg tagaatctat cccagctgat atccgtcaag cacttatcaa
    28981 caatggtggc ggacacttga accacgctct tttctgggaa ttgatgactc ccgagaaaac
    29041 agctccttca gcagaactgg cagcagcaat cgatgcaaca tttggttcat ttgaagaatt
    29101 ccaagcagcc ttcactgcag cagcaacaac tcgttttggt tcaggttggg catggttggt
    29161 tgtcaacaaa gaagggaaac ttgaagtgac ttcaacagca aaccaagaca caccaatctc
    29221 agaaggtaaa aaaccaatct tgggcttgga cgtttgggaa catgcttact acgtgaaata
    29281 ccgcaacgtg cgtcctgact acatcaaagc tttcttttca gtaatcaact ggaataaagt
    29341 agatgaattg tacgcagctg ctaaataatg atagttggag ggaagaattg ttcttctctt
    29401 ttttaggtta taagattctg gtctgacaaa atcgtcagac ttttttcatt tttatgagaa
    29461 acgtgctaac tgctagaaat tatggtagaa taaagtatta agtaatcgtg gaaaaaggat
    29521 atctatgcga aaagaaattg cacctgaatt atacaattat aacaagtttc ctggtccgga
    29581 gttccattta cacggggaca aggtcgaaac ggaagggata gctttttcct tggttgaaaa
    29641 tatcaaggat gcctttgatg tgacgacttt taatcagcgt ttttcagaag tcttaaccaa
    29701 gtttgattat atcgtggggg actggagcaa cgaacagctt cgcctacgag gtttttacaa
    29761 ggatgaccga acagaagaaa aacttgaaaa aatcagtcgt ttacaagact accttttaga
    29821 gtattgtagt tatggttgtg cctattttgt cttagaaaat gaagccccta agcgagcatc
    29881 atttgacaag aaaatgcgta agacggaaga agaaacacct tctagaaaag gaaagaaacc
    29941 gactcaaact aaacgaaagt cgaatgcaga taagaaaaat agacgtcgtc agaaagacca
    30001 gcattctcag aaagaggaca agggacaacg tcattttgtc attcgtcaga agtgagtaga
    30061 gaataaggag aagaaatgaa accctcaatt catagtttgg tacatcaaac catgcaggaa
    30121 tgggtattgg agcagggaga aaagaaattc cgtgcagatc aaatctggga atggctctac
    30181 cgtaaacgtg tgcagtcatt tgaagaaatg accaaccttt ccaaggattt gattgctaag
    30241 ctcaatgacc agtttgtggt caatcccttg aaacaacgta ttgtgcaaga gtctgctgat
    30301 ggtacagtca aatatctctt tgaattgccc gacggtatgt taattgagac tgtactcatg
    30361 cgtcaacact atggtttatc tgtctgtgtg accactcagg tcggctgtaa tatcggttgt
    30421 accttctgtg cctctggttt gattaagaag cagcgtgacc tcaataacgg tgaaatcgta
    30481 gcgcaaatca tgctggttca gaaatacttt gatgagcgtg gtcaggatga acgcatcagc
    30541 catatcgttg tcatgggaat cggtgagccc tttgataatt acaacaatgt cttgaatttc
    30601 gtttgtacta tcaatgatga taaaggaatg gctatcggtg ctcgtcacat cacgatttca
    30661 acctcaggtt tggctcataa aattcgtgac tttgctgatg aaggggttca ggtcaatctt
    30721 gccgtgtctc ttcacgcacc caacaatgaa cttcgttcaa gtatcatgaa aattaaccgt
    30781 gcctttccga ttgaaaaact ctttgctgct attgaatact atattgagac aacaaaccgt
    30841 cgtgtaacct ttgaatatat catgcttaat gaagtcaacg acggtgtaga acaagccttg
    30901 gaattggctg aattgctcaa gaacatcaag aaattgtctt atgtaaactt gattccttat
    30961 aacccagtta gtgagcatga ccaatatagc cgtagtccca aagagcgcgt gttggctttc
    31021 tatgatacgc ttaagaaaaa aggggtcaac tgtgtggttc gtcaagagca tggtacagat
    31081 attgacgcag cttgtggaca attgcgctct aacacaatga aacgtgaccg ccagaaggca
    31141 gtcgcagcgg taaatcctta atgatgaaga agacttataa tcatattttg gtctggggag
    31201 tcattttcta tagcatttgc attgtctgtt tttgctttac tcctcaagaa caatctaccg
    31261 tgggagtggg aactccaggt attcagcatc ttggacgcct ggtttttctt ttgactcctt
    31321 tcaattctct ctggaaactg ggcgaagtga gtgacattgg acaattatgt tggatttttt
    31381 tacaaaatat cctcaatgtc ttcttgtttt ttcctctgat tttccaactc ctttatctat
    31441 ttccaaattt gcggaaaaca aaaaaggtcc ttctttttag ttttcttgtg agtcttggaa
    31501 tcgagtgtac gcaattaatc ttggactttt tctttgattt caatcgcgtc tttgagattg
    31561 atgatttgtg gaccaacact ttgggtggct atctggcttg gctcctttat aaacgattac
    31621 ataaaaacaa ggtaaggaat taaaatgagt attttagaag ttaaaaatct gagtcacggt
    31681 tttggtgacc gtgcaatttt tgaagatgtg tccttccgtc tcctcaaggg agaacatatc
    31741 ggcctggtcg gtgccaatgg tgaaggaaaa tcaaccttta tgagtatcgt gactggtaaa
    31801 atgctgccag atgaaggaaa ggttgagtgg tccaaatatg tgacggctgg ttacttggat
    31861 cagcactctg tccttgctga aagacagtcg gtgcgtgatg ttctccgtac ggcttttgat
    31921 gagcttttca aagctgaagc tcgtatcaat gacctctata tgaaaatggc tgaagacggc
    31981 gcggatgttg atgctctcat ggaagaagta ggagaacttc aagaccgtct ggagagtcgt
    32041 gatttctata ccttggatgc taagattgac gaagtagcgc gtgctcttgg tgttatggac
    32101 tttgggatgg atacggatgt aacttctttg tcaggtgggc aaagaaccaa ggtgcttttg
    32161 gcaaaacttc tccttgaaaa gcctgatatt ttgctgttgg acgagccgac caactacttg
    32221 gatgctgagc atattgattg gctcaagcgc tatctccaaa actatgagaa tgcctttgtt
    32281 cttatttcgc acgatattcc attcctcaat gacgttatta atattgtcta tcatgtggaa
    32341 aatcaacagc tgacgcgtta ctctggtgac tactaccagt tccaagaagt ttatgctatg
    32401 aagaaatctc agctagaggc agcctacgaa cgccagcaga aagagattgc agacctcaag
    32461 gactttgtgg ctcgtaataa agcccgtgtt gcaactcgta atatggctat gtctcgtcaa
    32521 aagaaattgg ataagatgga tattatcgaa ctccaaagtg agaaaccaaa accatccttt
    32581 gatttcaaac cagctcgtac accagggcgc tttatcttcc aagccaagaa cttgcaaatt
    32641 ggttacgacc gtcctcttac taagccttta aatcttacct tcgaacgcaa tcaaaaggtt
    32701 gcgattattg gtgctaatgg tattggaaaa acaactctct tgaagagtct cttaggcatt
    32761 atctcgccaa tcgctgggga agtggagcgt ggagattatt tagaacttgg ttattttgag
    32821 caggaagtag aaggcggtaa tcgccaaact cctcttgaag ctgtctggaa tgcctttcct
    32881 gcccttaatc aagcagaagt ccgtgcagcc cttgcccgtt gtggtttgac aaccaaacat
    32941 attgaaagcc agattcaagt attatcaggg ggagagcaag ccaaggttcg tttctgtctc
    33001 ttgatgaatc gtgaaaacaa cgttttagtg ctggacgagc cgaccaacca tttggatgtg
    33061 gatgcaaagg atgagctcaa acgcgctctc aaagaataca ggggatctat ccttatggtc
    33121 tgccacgagc cagactttta tgaaggttgg atagaccaaa tatgggattt taataattta
    33181 acttaagaat gagagtaaaa gaaatacagt accgaatgtg gatactgtat ttttttggtt
    33241 tttaagattt aaaatcgtag tctttcacca cttcgatgct gtcgattgtg atagcagtag
    33301 ttggcttgtc tttttcatct ttttcggctt tagcaatctt atccacaaca tccataccgt
    33361 caatcacttg accaaagact gggtgtttgc catctagact agggtttcca ccttctttgt
    33421 aggcttcgat aattttctgt ggatacttgc ttgtagggag tttagaagag gtatctgtag
    33481 agttttggtt gatgaagaac tggctgccat tggtgtttgg ttgaccagta ttagccatag
    33541 caagagcacc acggatgtta tacaaataag gagtaatctc gttcttgaaa ccagttcctt
    33601 tgtctttagt cttatccttg tcatgccata tggactgacc acctgtaccg tccccttttg
    33661 gatctccagt ttggaccata aagccatcga tgacacggtg gaaggtaata ccgttatagt
    33721 agccttcttt ggcgtgagtg aggaaatttt caaccgctag aggagcgagt ttagggaaga
    33781 gtttaatgcg aatatcacct tggcttgtgt ggagaatcac ttcggcttca tcttcagcaa
    33841 cttccttaga tagttgtggg aaattggcgt tttcgtttgt taaagcatcg tttaactcct
    33901 tggcagattg ggcagctact ttggaacttt cttcagcagc aagactggaa tcaacataat
    33961 catcaccacg cagactgcgt tggacgctgc tacacccagc tagggctaca gtagacagta
    34021 aaagaagggt tgctagtttt ttcattctga tcctctcaaa aattcattta taccattgta
    34081 ccctaaaagg aagtggattt caaatattag tagtttacag aactggctta gataatagtt
    34141 gggatttcat catcatcttg aaaatcttca acttcttcaa cttcttcccc agtaaatggt
    34201 ttctgaaaag aacttagtat aaaacgggca attggttcaa caattaggaa atttagagga
    34261 agagctgcaa taaagtttac gaaccaggcg cttagatagg tattgggtgt gatagcaatg
    34321 ccattgtaaa aaagtccata gaaggacatg aaagtgacca gctaattctt cttctgaaaa
    34381 atgctcatga agggcttgat ccaggtcgca agatttcatt ttagaaagtc cagtaactcc
    34441 cttcttcttc aggatattct ttttcatggt attatttaga tggtcaagtg agtcaaaaga
    34501 agtttcgaca agagcatacc ctttttctac cagagttttt agatgtttag gggcatcaat
    34561 accataagtg tactcaaagt attttggaaa ccagatctta agaaagctcg taaagctagc
    34621 cagttctcta aacgctaaga actagctaca acacagcatt tacaacactt catggttcac
    34681 accatggggt gttttttaat gattttaagc aaaattcaca cttaattgaa agttatttaa
    34741 attagatgaa aatttatttt ttgaaaaagt gggaaaacat tgattctaag cgaattttga
    34801 atcatattcg aatgggagtt tttaaaatca tgtttcagtg gtaaaagtac caaactggat
    34861 tctccatagt aaaatgatat ctcctgctag tgaatcatct gctaataagt ccatatttcg
    34921 cttgggactt agaagccaag tatccaactc tcgttcaggc gatatgaagg gtttgacttg
    34981 gtgttcttta taaaattttt ctaaaatagt attcacggat agcatctttc aaacatttat
    35041 ttcctattct aacaaatttc taaatgcttt aagaaattta tagtctgtca agtttttttc
    35101 ttgacacctg ccagaaatct gctataatag aacatgtgct aaatagctca gctatttcac
    35161 cgaaataaaa aattaaagaa atgagacttt aaaaatggca gttaaaatcc gtttgactcg
    35221 tatgggttct aagaaaaaac ctttctaccg tatcaacgta gcagattcac gttcaccacg
    35281 tgacggacgt ttcatcgaaa cagttggaac ttacaaccca cttgttgctg aaaaccaagt
    35341 aactttgaaa gaagaccgcg ttcttgcatg gttggctaat ggagctcaac cttcagacac
    35401 agtacgtaac atcctttcaa aagaaggcgt attgaaaaaa ttccacgatt ctaaattctc
    35461 aaaataagtt taaagtaggt tgacagatgg atacgattga aaatctcatt attgcgattg
    35521 tgaaaccctt aatttcacaa ccagatgcct taactatcaa gattgaggat acaccagaat
    35581 ttttggaata tcatttgaat cttgatcaaa gcgatgtggg tcgtgtaatc ggtcgtaagg
    35641 gtcgcactat ttctgcgata agaacgattg tctactctgt cccaactgaa tacaaaaaag
    35701 taagaatcgt tattgacgaa aaataagaag ggcgggacgg atgtctcgct tttttgtgtg
    35761 gaatagaaga tatagtaaaa tgaaataata acaggacaaa tcgatcagga cagtcaaatc
    35821 gatttctaac aatgttttag aagtaaaggt gtactattct agtttcaata tactataaaa
    35881 gattgaacgt ttaggcaatt ccaagactac ttaatcgaac attaacagca gtctcggact
    35941 gatgaaggtc tttttatgaa gttagtggag gaagtcggag aagtagccga ggttttgaat
    36001 aggcgctctg gtcgaaaaga agggatagga ttcgaacgag gaacttgcca gagaactggc
    36061 tgatatcatt tactacaccg tcgcaatcgt agctattaat catattgacc ttaccaagac
    36121 tgtctttgag aaagacaaaa ccgcagccgt taattaccaa cataaacata atttggaagg
    36181 attattgaag ggaaagtaga gatattgtcc ccttcctcca acttgagaaa atccttggaa
    36241 cgtgataaaa taaaggataa ggatttttaa taattcatta tatagaatca gtggatttct
    36301 ttatgaagag gtttgaagta tctacagaaa ttggtagcct ctctgttact tatcaaaagc
    36361 aaaagaaagt gctagtttgt ctaaacggag caggtttgct accaagttat gaaaattttt
    36421 cacttatact tgaaaaacct cctcctacaa ttggttattt gacaattgat tttccgaaca
    36481 caggtaggag tccgattcat gaccaagctg gaaaaaatct agataatctt gcagatgcgg
    36541 tttatgaagt acttgaagaa ttaggaattt ctgaatatat actttgtgca catagttgga
    36601 gtggaatttt agcttgcaaa ttactcgaga aaccaattaa gcgtcagact ttagtagcaa
    36661 ttgaaccgac aactaaaaaa gtcatgtttg ctgatttttc agaaaatcct tatccagaaa
    36721 tggaagagca gatgaggctg attgacgagt gtggtcctga actttatttt aagaacttaa
    36781 ctcaagcaac atttagtcct gaaacgaata aaaaaatctg ggaattaatg caagaaaaag
    36841 gcttagagtt ggaaaatcaa gatccagaat ttcagatatc tggagagatt actgaggaag
    36901 attttgagga tgtatctata gaagctcata ttcctgtatt tgttttttgt cagccttata
    36961 gagaaaaaga gtacagagaa tcagaatatt ggacttccaa tactaaactc attttaggag
    37021 ggaatcacca ttatttacaa tggtcagaat cggaaaaaat tgcggctatt attcgagaat
    37081 tgttagaata agatggaaaa aaggagatta caggagacaa gatgaactac tttaatgttg
    37141 ggaaaatcgt taatacgcag ggattacagg gtgagatgcg agtcttgtct gtgacggatt
    37201 ttgcagaaga acggtttaaa aaaggagctg agctggcttt gtttgatgaa aaagatcagt
    37261 ttgtccaaac agtgaccatc gctagccacc gtaaacagaa gaactttgac attattaaat
    37321 tcaaagatat gtaccatatc aatactatcg aaaagtacaa gggatacagt ctcaaggtcg
    37381 ctgaggaaga tttgaatgac ctagacgatg gtgaatttta ctatcacgag attatcggtt
    37441 tggaagtcta tgagggtgat agcttggttg gaaccatcaa ggaaatcctg caaccaggtg
    37501 ctaatgatgt ctgggtggtc aaacgaaaag gcaaacgtga tttgctttta ccttatatcc
    37561 caccagtggt tctcaatgtt gatattccaa ataaacgggt cgatgtggaa atcttagaag
    37621 ggttagacga tgaagattga tattttaacc ctctttccag agatgttttc tccactggag
    37681 cactcaatcg ttggaaaggc tcgagaaaaa gggctcttgg atatccagta tcataatttt
    37741 cgagaaaatg ctgaaaaggc ccgtcatgta gatgatgagc cctacggagg cggtcagggc
    37801 atgttgctca gagtacaacc tattttcgat tcctttgatg ctattgaaaa gaaaaatccg
    37861 cgcgttattc tcctcgatcc tgctggaaag cagtttgatc aggcttatgc tgaagatttg
    37921 gctcaagagg aagagctaat ctttatctgt gggcactatg agggttatga tgagcgcatt
    37981 aagaccttgg taacagatga gatttcccta ggcgactatg tcctcactgg tggagaattg
    38041 gcagctatga ccatgattga tgctacagtt cgcctgattc cagaagtgat tggcaaggag
    38101 tctagccacc aagatgatag tttttcttca ggtcttttag aatatcctca gtacacacgt
    38161 ccttatgatt atcgaggcat ggtcgtgcca gatgtattga tgagtggcca ccatgaaaag
    38221 attcgtcagt ggcgattgta cgagagttta aagaaaacct acgagcgcag accggattta
    38281 cttgaacatt atcaactgac agtagaagaa gaaaaaatgc tggcagaaat caaagaaaac
    38341 aaagaataaa ggagaaacct atgcaagtaa tcaaacgtaa tggcgaaatt gctgaattta
    38401 atccagataa gatttaccaa gccatcttga aggcagccca aactgtttat gttttgacag
    38461 atgatttgcg tcaaaatctt gctcaagtca ctaagaaggt agttttggat ttacaagaag
    38521 ccaaggtgga acgtgcgact atcagtatga ttcaatctat ggttgaacat cgtttattgg
    38581 gcgcaggtta cattaccatt gcagaacact acatttccta tcgtttacaa cgtgacttgg
    38641 aaagaagtgg ttatggagat catatcgcag ttcatttaca ttttgaacaa attcgctaag
    38701 aaaaaagagt gggatgaagc aactacatct cactctttct taaatctatt tttttgggct
    38761 gtttggacag ttgaggggac aaatcgcagg cgttgaatat cgcttatacg gataatccgg
    38821 ctcacatttt tggaaaaatt tttcacgata atttgctggc cttgctgatc atatttgatg
    38881 atgtcacctg taaagcttgt ctcagataag ataaggtgaa cagcggtttg tttctggata
    38941 gcctcttcaa tagtggctgt aagggagttg gtttcagtct ggtcaggttg gtcatatcca
    39001 tttaaaaaat catgtatttt atttagaact tgctggaatt tatgtctcat attggcctcc
    39061 cttcttttat atcaatatta tataaaattt tcctaaaatc tacaagattt tacgaataaa
    39121 ctaatgaaac taaaaaagga gaagaaaatg gcagaattta catttgaaat cgaagagcac
    39181 ttgttgactc tttctgaaaa cgaaaaaggt tggaccaagg aaattaaccg tgtgagcttt
    39241 aatggtgccc ctgcaaagtt tgatattcgt gcttggagtc cagaccatac taaaatgggc
    39301 aaagggatta ctctctcaaa tgaagaattt caaacgatgg tggatgcctt taaaggcaac
    39361 taatactctt cgaaaatctc ttcaaaccgc gtcagctttg ccttgccgta tgtatggtta
    39421 ctgacttcgt cagtttcatc cacaacctca aaacaatgtt ttgagctgac ttcgtcagtt
    39481 tcatccacaa cctcaaaaca atgttttgag ctgacttcgt cagtttcatc cacaacctca
    39541 aaacagtgtt ttgagcagcc tgcggctagc ttcctagttt gctctttgat tttcattgag
    39601 tataagtttt caaaataaaa gaaaaggata ttgagtcatg ataactcggt atcctttttt
    39661 agttagtaaa gtaagcctga tttttaaggc gttgaagtag gggacggcta ataaaactaa
    39721 tagccagaag tttgccaagg tctggaatga taaagggaag aacccccaca acaagagctt
    39781 tttcaaatgc cattccagct aggaaatgca agctgagaat cccgccgaca aagacaaggg
    39841 catcacccaa gaggtttgca agaaaaatct taacaacacc actcttgctg ttggttagag
    39901 aggaagtaag tccagagtaa acgagataaa accaaagata gcctgcagta gggccaacta
    39961 aagcctgaaa accagctcca cctcctgcaa agacaggaag accgatagca cctagaagaa
    40021 gatagagtcc agcagaaagt acagcctctc tcggtctaaa gacagtagca atcaagccga
    40081 ttgcaaagtt ttgcagagtg aagggaacag gtccaattgg aagactgatt tgtgccaaag
    40141 cagcaatgag agcagcccca atagcaggga tagcataaac gtgagctttt ttcaaaatag
    40201 ttaacctctt ttctaatgta tagtaactat tatactcata taggagtcga tgtcaaccat
    40261 tttttaaaaa caaggtaaca aaaactgtaa cagccgattt tcgtgactag tcaagttgga
    40321 taattttttt gttgattcga aaatcattaa aaaagaagca gacatattta tatcagcttc
    40381 ttttgtttcg attaacgcat ggttacaaat tcttcagatg cagttgggtg aattgccaca
    40441 gttgcatcaa agtcagcctt ggttgctccc attttgatag caacggcaaa tccctgaatc
    40501 atttcatcaa cgccgtagcc aattccatga agtccgacaa ctttttcttc tgaaccagct
    40561 gttatgagtt tgaaacggga ttcttgacgg ttgcgagtgc aagcagagta catagatgca
    40621 aagcttgatt tataaacctt gatttggtct tgaccgtatt ctttaatagc ttgctcttct
    40681 gtcaaaccaa cagttccgat agcagggtgt gaaaagacaa cagttggaat agttgagtaa
    40741 tccatttttg cagtagtttt tccgttaaag agacgttcag atagggtacg tccggccttg
    40801 attgcaactg gagtcagttc tttctcgccc gttacatcac ctagagcata gagtccctca
    40861 acaacagtat tttggtattc atccacttgg ataaagccac gttcgttcag agtcactcca
    40921 gctttttcaa gttgcaagcc cttaacgttt ggacggcggc ctgtagccca gataacttgg
    40981 ctagctgtgt gactagtacc atcttcgaaa tgaatggtaa tgccgtcagt agttttttct
    41041 aacttgacag ggactttgtg agtgtgaagt ggtaagtttg ttctttccat ttccttgacc
    41101 aaaccttcaa cgatgtagga atcaaaacca cgtaaaggac gatcgcggcg aacaaagaga
    41161 tctgtcttga caccaaaagt gtggagtacg ccagctaatt caacggcgat ataaccagcg
    41221 cctagaatgg caactgactc tggaagttct tcccaggcaa atacatcatc agaagagcca
    41281 cctagctcag caccaggaat attaggaata cttggatggg cacctgtagc aatcacgata
    41341 tgtttagcac gaatcagttc accatttacg cttacagtat gagaatctac aaattcagca
    41401 tgaccttcaa tcaagtctac accgttgcgt ttaaaactac catcataaga agaacgagcg
    41461 cgatcaatgt aggcttcacg attgcgacgt agggttgcaa agtcaaagtt aagatcagta
    41521 gtcttaaagc cgtagtcttc tccaaattga tggaaagtct cagcgatttg cgccccgtac
    41581 cacatgattt ttttaggaac acaaccgacg ttgacacagg ttccacctaa tttcttttcc
    41641 tcaataacgg ctgctttggc tccatgttca ccagcacggt tcatggtagc gattcctccg
    41701 ctacctccac cgatagcaat gatatcatat tctctcattg aaaactcctt tgtaatcttt
    41761 taaatagtag agcgtctttt ttgatagtta tattttattc tttttttgat ttgattgcaa
    41821 aaatctgcta cgcacaaaaa attgcaaaaa agcttgccaa ataagtcaag atagtgtatt
    41881 atatatctag tacaatagaa aagcaaaatt ccgaacgatt gtttaatcct gaactgccat
    41941 tatttccatc tgaactgcat tgtttgctgg agggaaattt ggtataatag caaagtattg
    42001 taaaaaagaa aaggagtttc attatgaaga aaaagaatgg taaagctaaa aagtggcaac
    42061 tgtatgcagc aatcggtgct gcgagtgtag ttgtattggg tgccgggggg attttacttt
    42121 ttagacaacc ttctcagact gctctaaaag atgagcctac tcatcttgtt gttgccaagg
    42181 aaggaagcgt ggcatcctct gttttattgt cagggacagt aacagcaaaa aatgaacaat
    42241 atgtttattt tgatgctagt aagggtgatt tagatgaaat ccttgtttct gtgggcgata
    42301 aggtcagcga agggcaggct ttagtcaagt acagtagttc agaagcgcag gcggcctatg
    42361 attcagctag tcgagcagta gctagggcag atcgtcatat caatgaactc aatcaagcac
    42421 gaaatgaagc cgcttcagct ccggctccac agttaccagc gccagtagga ggagaagatg
    42481 caacggtgca aagcccaact ccagtggctg gaaattctgt tgcttctatt gatgctcaat
    42541 tgggtgatgc ccgtgatgcg cgtgcagatg ctgcggcgca attaagcaag gctcaaagtc
    42601 aattggatgc aacaactgtt ctcagtaccc tagagggaac tgtagtcgaa gtcaatagca
    42661 atgtttctaa atctccaaca ggggcgagcc aagttatggt tcatattgtc agcaatgaaa
    42721 atttacaagt caagggagaa ttgtctgagt acaatctagc caacctttct gtaggtcaag
    42781 aagtaagctt tacttctaaa gtgtatcctg ataaaaaatg gactgggaaa ttaagctata
    42841 tttctgacta tcctaaaaac aatggtgaag cagctagtcc agcagccggg aataatacag
    42901 gttctaaata cccttatact attgatgtga caggcgaggt tggtgatttg aaacaaggtt
    42961 tttctgtcaa cattgaggtt aaaagcaaaa ctaaggctat tcttgttcct gttagcagtc
    43021 tagtaatgga tgatagtaaa aattatgtct ggattgtgga tgaacaacaa aaggctaaaa
    43081 aagttgaggt ttcattggga aatgctgacg cagaaaatca agaaatcact tctggtttaa
    43141 cgaacggtgc taaggtcatc agtaatccaa catcttcctt ggaagaagga aaagaggtga
    43201 aggctgatga agcaactaat tagtctaaaa aatatcttca gaagttaccg taatggtgac
    43261 caagaactgc aggttctcaa aaatatcaat ctagaagtga atgagggtga atttgtagcc
    43321 atcatgggac catctgggtc tggtaagtcc actctgatga atacgattgg catgttggat
    43381 acaccaacca gtggagaata ttatcttgaa ggtcaagaag tggctgggct gggtgaaaaa
    43441 caactagcta aggtccgtaa ccaacaaatc ggttttgtct ttcagcagtt ctttcttcta
    43501 tcgaagctca atgctctgca aaatgtagaa ttgcccttga tttacgcagg agtttcgtct
    43561 tcaaaacgtc gcaagttggc tgaggaatat ttagacaagg ttgaattgac agaacgtagt
    43621 caccatttac cttcagaatt atctggtggt caaaagcaac gtgtagccat tgcgcgtgcc
    43681 ttggtaaaca atccttctat tatcctagcg gatgaaccga caggagcctt ggataccaaa
    43741 acaggtaacc aaattatgca attattggtt gatttgaata aagaaggaaa aaccattatc
    43801 atggtaacgc atgagcctga gattgctgcc tatgccaaac gtcagattgt cattcgggat
    43861 ggggtcattt cgtctgacag tgctcagtta ggaaaggagg aaaactaaga tgcagaatct
    43921 gaaatttgcc ttttcatcta tcatggctca caagatgcgt tctttgctta ctatgattgg
    43981 gattattatc ggtgtttcat cagttgttgt gattatggct ttgggtgatt ccctatctcg
    44041 tcaagtcaat aaagatatga ctaaatctca gaaaaatatt agcgtctttt tttctcctaa
    44101 aaaaagtaaa gacgggtctt ttactcagaa acaatcagct tttacggttt ctggaaagga
    44161 agaggaagtt cctgttgaac cgccaaaacc gcaagaatcc tgggtccaag aggcagctaa
    44221 actgaaggga gtggatagtt actatgtaac caattcaacg aatgccatct tgacctatca
    44281 agataaaaag gttgagaatg ctaatttgac aggtggaaac agaacttaca tggacgctgt
    44341 taagaatgaa attattgcag gtcgtagtct gagagagcaa gatttcaaag agtttgcaag
    44401 tgtcattttg ctagatgagg aattgtccat tagtttattt gaatctcctc aagaggctat
    44461 taacaaggtt gtagaagtca atggatttag ttaccgggtc attggggttt atactagtcc
    44521 ggaggctaaa agatcaaaaa tatatgggtt tggtggcttg cctattacta ccaatatctc
    44581 ccttgctgcg aattttaatg tagatgaaat agctaatatt gtctttcgag tgaatgatac
    44641 cagtttaacc ccaactctgg gtccagaact ggcacgaaaa atgacagagc ttgcaggctt
    44701 acaacaggga gaataccagg tggcagatga gtccgttgta tttgcagaaa ttcaacaatc
    44761 gtttagtttt atgacgacga ttattagttc catcgcaggg atttctctct ttgttggagg
    44821 aactggtgtc atgaacatca tgctggtttc ggtgacagag cgcactcgtg agattggtct
    44881 tcgtaaggct ttgggcgcaa cacgtgccaa tattttaatt cagtttttga ttgaatccat
    44941 gattttgacc ttgttaggtg gcttaattgg cttgacaatt gcaagtggtt taactgcctt
    45001 agcaggtttg ttactgcaag gtttaataga aggtatagaa gttggagtat caatcccagt
    45061 tgccctattt agtcttgcag tttcggctag tgttggtatg atttttggag tcttgccagc
    45121 caacaaggca tcgaaacttg atccaattga agcccttcgt tatgaatgag atcaacaaga
    45181 tggacactcg tctatcttgt ttttgtatgg atttccctat cgaaattcat taaaaatgtg
    45241 atataatgaa gtgttagaaa aacaggtttc atttgcctgg aaaggaaaaa ttatgtctga
    45301 aaaaaatttt tatattacaa cgccgattta ctatccatct gggaaacttc atatcggttc
    45361 tgcctacaca actatcgcat gtgatgtcct agcacgttac aaacgcctga tgggctacga
    45421 tgtattttat ctgacaggtc ttgatgaaca tggtcagaaa atccagcaga aagcggaaga
    45481 agctggtatt acacctcaag cctatgttga tggaatggcg gttggagtta aagaactctg
    45541 gcaattacta gatatctcat acgataaatt tatccgtaca actgatgact accatgaaaa
    45601 agttgttgca caggtctttg aacgcttact tgctcaagat gatatctact tgggtgaata
    45661 ctctggttgg tattcagtat cagacgaaga attctttaca gaaagccagc tggcagaagt
    45721 tttccgtgat gaagctggaa atgtgactgg tggtattgct ccatcaggtc atgaggttga
    45781 atgggtttct gaagaatcat acttccttcg ccttagcaaa taccaagacc gtttggtcga
    45841 atttttcaaa gctcatcctg aatttatcac gccagatggt cgccttaatg aaatgctacg
    45901 caacttcatc gagccaggtt tggaagattt ggcggtatct cgtacaacct ttacatgggg
    45961 agtgcctgtc ccatcaaatc caaaacacgt tgtctacgtt tggattgatg cccttcttaa
    46021 ctatgcgaca gctcttggct acgctcaaga cgaacatggt aactttgaca agttctggaa
    46081 tggaacagtc ttccatatgg taggaaaaga tatccttcgc ttccactcta tctactggcc
    46141 aatccttctt atgatgttgg atgttaaatt acctgatcgt ttgattgccc atggttggtt
    46201 tgtcatgaaa gacggaaaaa tgtctaagtc aaaagggaat gtcgtttacc ctgaaatgtt
    46261 ggtagagcgt tatggactag atccacttcg ttactacctc atgcgtaacc ttccagttgg
    46321 ttcagacgga acctttactc ctgaagacta tgtcggccgt atcaactatg aattggctaa
    46381 tgaccttggg aacctcctta accgtacggt ttccatgatt aataagtact ttgatggaca
    46441 aatccctgcc tatgtagaag gtgtgactga atttgatcat gttcttgctg aggttgcaga
    46501 acaatcaatc gcagacttcc atacacacat ggaagcagtt gactatccac gtgcgcttga
    46561 agcagtctgg actctgatct ctcgtaccaa taaatacatc gatgagactg caccatgggt
    46621 cttggacaag gatgaagctc ttcgtgacca attggcaagt gtcatgagcc acttggcagc
    46681 cagcattcgt gtagttgctc acttgattga accatttatg atggaaacta gtcgtgcagt
    46741 tttgactcaa cttggtttgg aagaagtttc tagtcttgaa aacttgagtt tggctgactt
    46801 cccagcagat gtgactgtag ttgccaaagg aacacctatc tttccacgtc taaatatgga
    46861 agaagaaatc gcctatatca aggaacaaat ggaaggcaat aaaccagcag tcgaaaaaga
    46921 atggaatccg gacgaagttg agctcaaact aaacaaggat gaaatcaagt ttgaagactt
    46981 tgacaaggtt gaaatccgtg tcgcagaagt caaagaagtg tctaaagtag aaggttcaga
    47041 taagttgctt caattccgct tggatgctgg tgatggagaa gatcgtcaga ttctttcagg
    47101 aattgcaaaa tactatccaa atgaacaaga attggtcggc aagaaagttc aaatcgttgc
    47161 taacctcaaa ccacgtaaaa tgatgaaaaa atatgtcagc cagggtatga ttctctcagc
    47221 tgaacatgat ggcaaattaa cccttctcac agttgatcca gctgtaccaa atggaagtgt
    47281 gattgggtaa aagtagacag gactagttca ggctagttct gtttttttat ttatactcaa
    47341 tgaaaatcaa agagcaaact aggaagctag ccgcgggttg atcaaagcac tgctttgagg
    47401 ttgtagatga aactgacgaa gtcagctcaa agcactgctt tgaggttgta gatgaaactg
    47461 a
//
LOCUS       DAVKMU010000011        47223 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKMU010000011 DAVKMU010000000
VERSION     DAVKMU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47223
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.011"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(4..300)
                     /locus_tag="VW343_000696"
     CDS             complement(4..300)
                     /locus_tag="VW343_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755291.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(284..2443)
                     /gene="pfbA"
                     /locus_tag="VW343_000697"
     CDS             complement(284..2443)
                     /gene="pfbA"
                     /locus_tag="VW343_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9755292.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYSGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVVN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKINNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3573..4799)
                     /locus_tag="VW343_000698"
     CDS             complement(3573..4799)
                     /locus_tag="VW343_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9755293.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFKNEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4842..5534)
                     /locus_tag="VW343_000699"
     CDS             complement(4842..5534)
                     /locus_tag="VW343_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9755294.1"
                     /translation="MLKWEDLPVEMKSSEVESYYHLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5707..7458)
                     /locus_tag="VW343_000700"
     CDS             complement(5707..7458)
                     /locus_tag="VW343_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755295.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDDQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(7448..9193)
                     /locus_tag="VW343_000701"
     CDS             complement(7448..9193)
                     /locus_tag="VW343_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755296.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLVFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9321..9851)
                     /locus_tag="VW343_000702"
     CDS             complement(9321..9851)
                     /locus_tag="VW343_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9755297.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            complement(9848..10304)
                     /locus_tag="VW343_000703"
                     /pseudo
     CDS             complement(9848..10304)
                     /locus_tag="VW343_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10438..11265
                     /gene="xth"
                     /locus_tag="VW343_000704"
     CDS             10438..11265
                     /gene="xth"
                     /locus_tag="VW343_000704"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9755298.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11277..11375
                     /locus_tag="VW343_000705"
     CDS             11277..11375
                     /locus_tag="VW343_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9755299.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            11420..11659
                     /locus_tag="VW343_000706"
     CDS             11420..11659
                     /locus_tag="VW343_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9755300.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12042..12137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12537..13118
                     /locus_tag="VW343_000707"
     CDS             12537..13118
                     /locus_tag="VW343_000707"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9755301.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13118..14380
                     /locus_tag="VW343_000708"
     CDS             13118..14380
                     /locus_tag="VW343_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9755302.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14430..14891)
                     /locus_tag="VW343_000709"
     CDS             complement(14430..14891)
                     /locus_tag="VW343_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU9755303.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(14888..15652)
                     /locus_tag="VW343_000710"
     CDS             complement(14888..15652)
                     /locus_tag="VW343_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9755304.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYYPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(15833..16237)
                     /locus_tag="VW343_000711"
     CDS             complement(15833..16237)
                     /locus_tag="VW343_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9755305.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVIGQRSEERRVRI"
     gene            complement(16307..17788)
                     /locus_tag="VW343_000712"
     CDS             complement(16307..17788)
                     /locus_tag="VW343_000712"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9755306.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWETYVHYPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDPAVQ
                     ILAETDRTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(17807..18985)
                     /locus_tag="VW343_000713"
     CDS             complement(17807..18985)
                     /locus_tag="VW343_000713"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9755307.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKETVGKHYEEVVGYAPSFYIAEVAGGTHVLD
                     "
     gene            19150..20157
                     /gene="galR"
                     /locus_tag="VW343_000714"
     CDS             19150..20157
                     /gene="galR"
                     /locus_tag="VW343_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9755308.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20203..21240)
                     /locus_tag="VW343_000715"
     CDS             complement(20203..21240)
                     /locus_tag="VW343_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9755309.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(21241..21594)
                     /locus_tag="VW343_000716"
     CDS             complement(21241..21594)
                     /locus_tag="VW343_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9755310.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(21838..22728)
                     /locus_tag="VW343_000717"
     CDS             complement(21838..22728)
                     /locus_tag="VW343_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9755311.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            22861..23397
                     /locus_tag="VW343_000718"
     CDS             22861..23397
                     /locus_tag="VW343_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9755312.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FIGQEVVQFYLDLLIPKN"
     gene            complement(23563..24369)
                     /locus_tag="VW343_000719"
     CDS             complement(23563..24369)
                     /locus_tag="VW343_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9755313.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(24714..26234)
                     /locus_tag="VW343_000720"
     CDS             complement(24714..26234)
                     /locus_tag="VW343_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9755314.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDSIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(26227..26955)
                     /locus_tag="VW343_000721"
     CDS             complement(26227..26955)
                     /locus_tag="VW343_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755315.1"
                     /translation="MIEYKNVALCYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(26969..27733)
                     /locus_tag="VW343_000722"
     CDS             complement(26969..27733)
                     /locus_tag="VW343_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9755316.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGATYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLKNGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(27737..28180)
                     /locus_tag="VW343_000723"
     CDS             complement(27737..28180)
                     /locus_tag="VW343_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9755317.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(28862..29158)
                     /locus_tag="VW343_000724"
     CDS             complement(28862..29158)
                     /locus_tag="VW343_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9755318.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(29180..30244)
                     /gene="pepA"
                     /locus_tag="VW343_000725"
     CDS             complement(29180..30244)
                     /gene="pepA"
                     /locus_tag="VW343_000725"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9755319.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALLGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            30645..31346
                     /gene="galE"
                     /locus_tag="VW343_000726"
     CDS             30645..31346
                     /gene="galE"
                     /locus_tag="VW343_000726"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9755320.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMDQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            31362..31472
                     /locus_tag="VW343_000727"
     CDS             31362..31472
                     /locus_tag="VW343_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEU9755321.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            31769..32728
                     /locus_tag="VW343_000728"
     CDS             31769..32728
                     /locus_tag="VW343_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755322.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFISSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            32718..33674
                     /locus_tag="VW343_000729"
     CDS             32718..33674
                     /locus_tag="VW343_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9755323.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            33671..34423
                     /locus_tag="VW343_000730"
     CDS             33671..34423
                     /locus_tag="VW343_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9755324.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            34519..35484
                     /locus_tag="VW343_000731"
     CDS             34519..35484
                     /locus_tag="VW343_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9755325.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETSTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(35709..35951)
                     /gene="yidD"
                     /locus_tag="VW343_000732"
     CDS             complement(35709..35951)
                     /gene="yidD"
                     /locus_tag="VW343_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9755326.1"
                     /translation="MKRILIALVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(35951..36673)
                     /locus_tag="VW343_000733"
     CDS             complement(35951..36673)
                     /locus_tag="VW343_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9755327.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(36660..37229)
                     /locus_tag="VW343_000734"
     CDS             complement(36660..37229)
                     /locus_tag="VW343_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9755328.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQALDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(37248..37976)
                     /locus_tag="VW343_000735"
     CDS             complement(37248..37976)
                     /locus_tag="VW343_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9755329.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(37976..38716)
                     /gene="xerD"
                     /locus_tag="VW343_000736"
     CDS             complement(37976..38716)
                     /gene="xerD"
                     /locus_tag="VW343_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9755330.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQRRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(38707..39168)
                     /locus_tag="VW343_000737"
     CDS             complement(38707..39168)
                     /locus_tag="VW343_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9755331.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(39165..39686)
                     /locus_tag="VW343_000738"
     CDS             complement(39165..39686)
                     /locus_tag="VW343_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9755332.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(39662..40633)
                     /locus_tag="VW343_000739"
     CDS             complement(39662..40633)
                     /locus_tag="VW343_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9755333.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(40630..41424)
                     /gene="racE"
                     /locus_tag="VW343_000740"
     CDS             complement(40630..41424)
                     /gene="racE"
                     /locus_tag="VW343_000740"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9755334.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(41631..41879)
                     /locus_tag="VW343_000741"
     CDS             complement(41631..41879)
                     /locus_tag="VW343_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9755335.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(42135..43760)
                     /gene="treC"
                     /locus_tag="VW343_000742"
     CDS             complement(42135..43760)
                     /gene="treC"
                     /locus_tag="VW343_000742"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9755336.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQTLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(43938..45905)
                     /gene="treP"
                     /locus_tag="VW343_000743"
     CDS             complement(43938..45905)
                     /gene="treP"
                     /locus_tag="VW343_000743"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9755337.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFVYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            46090..46800
                     /gene="treR"
                     /locus_tag="VW343_000744"
     CDS             46090..46800
                     /gene="treR"
                     /locus_tag="VW343_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9755338.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            46955..>47223
                     /locus_tag="VW343_000745"
     CDS             46955..>47223
                     /locus_tag="VW343_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9755339.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIIKKDTHKEIIAKLDYEAPSCP
                     ECGSQMKKYYFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKIMV"
BASE COUNT        13834 a        10721 c         8843 g        13825 t
ORIGIN      
        1 ttattatttc attttactat actctgttaa ttcatatgag tttaaaccga tttcatcttt
       61 aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt tacaattaac
      121 cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta tctcctcttt
      181 agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt gtgaacctac
      241 aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta tctttaccat
      301 aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt aagaatagtt
      361 ccataatctt gttcgaacca gtctttggta atttttgttt tacatctact atttctttag
      421 atttattaat atgattttca gtttctctgc catctccaac tattttatag tttacttctt
      481 ctgtcttatt atcttgttta ttgttgatct tgtcattcat ttgtctatta tctttacttg
      541 agttaaactc tccgttcttc tggttactat caattacatt atttgaatta gattgttttt
      601 cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca attttgtaaa
      661 gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca ggttctttcc
      721 catttgcatt agatttgatg aatgatatat acttaccgga taaattataa aattggttat
      781 ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta ccaccgaata
      841 tactattctc tgagactact aaattatcac taactcgtaa taattcaata tttggttgtt
      901 ctgtttcctt agaattatta ttaactacat tttttgttac agtaatatct gatacttttc
      961 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
     1021 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
     1081 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
     1141 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
     1201 cacctgaata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
     1261 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
     1321 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
     1381 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
     1441 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
     1501 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
     1561 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
     1621 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac
     1681 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
     1741 ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg
     1801 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
     1861 aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
     1921 ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca ccataatctt
     1981 ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt
     2041 ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt ctgttgtcta
     2101 attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt aacgaattat
     2161 ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattc tgattaacag
     2221 ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta gccatcggtg
     2281 aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca
     2341 atagtaccga gcaagtccct acctttaatt tacgaatact gaatacctca ttaggaacaa
     2401 aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct
     2461 taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc aaattgaatt
     2521 gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta
     2581 ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta tagtagacgg
     2641 aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt gactgtcccg
     2701 atcgatttgt cctattctta tttcatttta ctatatctac aacctcaaag cagggttttg
     2761 agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat
     2821 ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttgtt gatatgttgt
     2881 aaattataaa tgaaagaaca aataccttgt catacaagca ttcgccaggg atatttacat
     2941 gaatatcgct catatggcgg attgaaacta aaacactaca atatatttct taaatagacc
     3001 ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga
     3061 atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcgg gagatctaat
     3121 gatgtatagc aagcatcact aaacatatag aatatttgta ttattataag atcaatatgc
     3181 tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa
     3241 caatattctg aaaacaaaaa aagacgcgag tttccacaat ctcactcatg gaaactcacg
     3301 tctttcaaaa aatagggtga attgaaaact agaacaagac actagatttt ctaaatgatg
     3361 tctagtaagg attctaaatg ctacaccaaa ttgctcacat cttatttcaa accacgggac
     3421 tctcacaaga aaggaaaatc ctataaaaat tctctctctg aggagaaaga ggcctcaact
     3481 acaggtgtaa actattatca attgaaaaca acagttttta cactcaatta gactttttag
     3541 ggacaggatc actttcgctt gactgtagtt tgtcattttt ttgataaagt tagcactttt
     3601 tcagaaactg ttttgaaagt ctcaatgata tagtctactt cttcatcgct taatttagta
     3661 tgaagaggga gggtaatttc attcttaaag aaggcatagg ccttaggata gttcgtcata
     3721 tcaaatccaa gattcttata ggctgtcaag agaggaagcg gtttgtagtg aacattactt
     3781 gcaattcctg ctttagccaa ttcttggatg atgaggttgc gttcttctag gcttgctcct
     3841 tctacacggg tgatgtagag gtggcgtgaa gattcgacag tttcagtctt gtgtgccaaa
     3901 ggatggatgc gagaacctgc aaaaccacta tcatagcggt ccacaatgtc cttacggcgt
     3961 tgcaacaaac ttggatagcg gtccaattgt accaaaccaa gtgaagccat gatatcggtc
     4021 atgttgcact tataggctgg tgtaacgata tcgtattccc atgaccccag ttgcatcttg
     4081 gcaagagcat ccttagtttg cccgtgaagg gaaaggattt ggaattcctt gtacatctct
     4141 tcgtcatcaa tcactggatt ggctttccaa gtcgcacttc caccttctgc cgttgtaaag
     4201 ttcttaacgg catggaatga gaaggaagta aagtcagcga tagaaccaga aggttgtcct
     4261 ttataagtag atcccaaagc gtgggcacta tcagagacaa tgacaatacg gttaaaggcc
     4321 ttttgccact tgcttgaagc agtaaagaag tcacgttttt tctccacgac ttggaacaaa
     4381 cggtcataat cgcaaacaat ccctgcgagc tctactggga taatcacctt agttttctca
     4441 gtgatagctt gctcaagcag gtcatagtcc atctcaaacg tatctgcttg gatatccacc
     4501 atgacagggg ttgctcccac gtgcgtaatg acactacatg aagctgtata ggtcatggct
     4561 ggaacgatga cttcatcacc aggtcccact tccaaaacgc gtaaaatcaa ctccagagcg
     4621 gctgtcgcag agttgagaca aacagtctta ggtgtctgtg tgtaaagaga caagcggcgc
     4681 tccagttctt ttgttttagg acctgttgtg atccaaccag aacgcagggt atccgctact
     4741 tcagcaattt ctgcttctgt gatatcaggc ggtgaaaatg gaatattgta atttggcatt
     4801 gatttgctcc ttttatctgt actcattttc ttatgactac tttattttag tacctcaaac
     4861 acggtttgaa acatgatttt gatgtcccca aagaaactaa actctcggag ataggcgagg
     4921 ttatagcgca tcttttcagg aagaacatgc tccacatagg cctgatcaac tgacagacct
     4981 ttctccgtca tttgactgat gatggtgtcc tcatccttgt agttgatgct ggctggagag
     5041 gtaattcctg cttgcaagag caaggttgcc atcatttcag ggctatactg ctctgtataa
     5101 cgtggcactt caggtcgtgt accgacaaag gacatctcac ctttaaggac attgaccaac
     5161 tgaggcagtt cgtccaaacg gacacgtcgg atgaaatttc caaccttggt aatacggcta
     5221 tcgttagcag aagtcaccag acttcctttt ttatccgcat ccgtcaccat ggtacggaac
     5281 ttccaaatct tgaaccgacg gttgtactgg gtcacacgct cttgcttgta aatcactggc
     5341 cccttgctat ccaacttgat ccaaatgctc aagatgagaa agatgggaga ggttagaacc
     5401 agtaagacca aggccaaaaa ccagtccaag caacgcttga aaatcagcga acccttcctt
     5461 ttagagacaa gatggtagta agactcaacc tcgcttgatt tcatttccac aggcaagtct
     5521 tcccatttca gcatactgtt ctcctttgtt tttattatac tatttgtcaa catttttcat
     5581 tataccacaa aatggaaaag gcggtgaaag aaatctgtaa tttgaaggat ttccttctag
     5641 tactcaatga aaatcaaagt acagctttga gattgcagat aaagctgaca tggtttaaag
     5701 agattttcaa agagtatcgg ccatggcccc tgcctgcaaa ctatacatct tgtgataggt
     5761 tcctcccaga gtcaagagtt cctcatgggt tccactctcg ataatgcgtc ccttatccaa
     5821 gacatagatg cagttggcgt cttgaatagt agaaaggcgg tgagcgatag caatagttgt
     5881 tcggccctgt ctcatcttcg ccagagaagc ttgaaccaag ctttctgttt cagagtcaat
     5941 attggctgtc gcttcatcca aaatcaggat tttaggctgg ctggcgactg ttctagcaaa
     6001 ggcaagaagc tggcgctgcc cagtagagaa gctcgaacca cgctcggaaa caggggagtc
     6061 gtacccctga ggaagttctt gaataaagga atctgcatcc acaaaggctg ccgcagcctg
     6121 aacctgatca tcactgattt cttggtacat ggcgatattg gacttaatag ttccatgata
     6181 gaggaagggt tcctgcaaga ccaaaccgat gttttttctc agctcttctt gactgaaatc
     6241 cctgatatcc acatcatcca agagaactct ccctgactgg aattcataaa agcgcatgag
     6301 gacattgata atagacgatt tccctgaacc tgtatgacct acaaaggcaa tggtttcacc
     6361 cttattaaca gagaaagaaa tgtcatccag aatcggatgt ttaccgtcat atgagaaaca
     6421 cacatgttca aaacggatat tgccttcttg gactttggct tgcccatttt cttgaagagg
     6481 ttcataggtc ctctcgtcta tcagggcaaa gacacgacct gcagaaacca tagccgtttg
     6541 cagagttgaa aagttttgcg tcacctcaat caaggggtca aaaaggcggt tgatgtactg
     6601 gataaaggca tacatggtcc cgaccgttat cccgatagaa aaaccacggt agccaaagta
     6661 ggccatcaag actgcatagc ctagaagttt cagcaaactc atggcaggtc tcaaaaagag
     6721 ggcatccaag gctacagaac ggttggcgta gaccaagtgt tcttggttga tttcatcaaa
     6781 ttctgcctgc aggcgcttct cttgattaaa agcctgaata atcctgattc cctcgatatt
     6841 ctctgccagc ttactattga tatctgacaa gagacttctg gttttctcaa tgattttcac
     6901 tgattttttc cgatagagat tgaccaaaag gaaaatcaag gggagaaaga gcaagactaa
     6961 tgctgtcaaa cgaaaatcca ataccaacat ggtataaagg gttgtcagaa agatgaaaac
     7021 tgctgagata aagctggata aaatccccga aaacatatca ctgatagtct cggtgtcatt
     7081 tgtcaaacga gagacgatag agcctgctgg cgtcttgtca aagtaagaca tgcccagttt
     7141 ctccatattg gcaaaggcat cccgacgaat atccctaaca atactgtaag acacgcgcgc
     7201 aaagagaaga ttgcctacat actgaactac agtttgtagg atgtagagac catagtagac
     7261 cagcaaaacg gtaacggcta gttggttaag attgctgaga tactggtcga taaagtggga
     7321 agccacgagg ggtatgacac ttttaatgac cgtcgtcgct aggagaaaac tgagtgccaa
     7381 aaaggtcagg agtctataag gcttgagata agacatcaag cgcttcaata cagcccattg
     7441 ttcttgttta ttctgcatct tcttctcctt tcatttctaa ctgctgagac tggtaggttt
     7501 gggcatacca gccatccatc gctagcaagt cttcgtgcgt gcctcgttcg ataatttgac
     7561 cattttgtag aactaaaata aaatctgcat ggacaacagc actgaggcga tgggcagtga
     7621 taatggttgt cttgtccttt cgcgtctcct tgaggttgtc gataatcgca tactctgtct
     7681 tggcatctac ggcggataag gaatcatcca aaatcaagat atcagggtct aaaatcatag
     7741 cccgactcat agccaaccgt tgcttttgac caccagaaag actgactcct ttttcaccaa
     7801 tcagcgtatc aaatccttga ggcatgtcta caatatcttg gtaaactcgg gctagcttag
     7861 tagcttcctc gaccgctgaa aggggcaagt taggattgcc aaagcggata ttgtctagga
     7921 ttgaagttgc aaagagaaac tggtcctgag gaacatagcc catgagactg cgaaggtctg
     7981 tcagacgata gtcccgaata tcgtgaccgt ttagataaat ggcaccctta tccacatcgt
     8041 attcacgcaa gaggagcttg attaaggacg ttttcccaga gcctgtctgc ccaaccaagc
     8101 ccagtgtttg cccttttgcc aaactaaagt gaatatccgt cagtgtttcc tcattttcaa
     8161 aagcaaagct gtcaatggca tactccaaac gcccattttc aataccatcc agaggaaact
     8221 cagggtcttg tacaggagat tcctgagaca aaagattttc aatccgctgg taagaaacct
     8281 tccctcgctg agtagtatta aagaggaagc cgatggccat aagaggccag accagcatat
     8341 ccaaatagct gataaaggtg actagattcc caactgtaat ctgcccttcc tgaaccatca
     8401 aggagccaac caaaagcgtt aaaacatagg acgaaccaac aaacacgaga accatagggt
     8461 caaagagact atcatatttc atggtttgca ggttcttttg gaaggttaat tcattgactg
     8521 cctgaaaaga cttcaactcg tctgcctgat aaccgaaaga cttggtcact ttgatacctg
     8581 atacggactc ctgtaccttg ttattgagtt cagaaaaagc agcttgggat tcgccaaagg
     8641 ccttatgagt ctttctccct aggcgactag tcgcataggc catgaaaggt agggggagaa
     8701 tggcaacaag agtcatctgc catgagatgc taaagagcat ggtcaacaaa gtcaccagag
     8761 ccgtgataga ggcatccacc gcagacatga caccgccacc tgctaaacga gtcaaggcat
     8821 tgatatcatt ggttgcgtgt gccatcagat cacccgtccg ataggtttga taaaaggctg
     8881 atgacatttt tgtgaaatgc ttaaacaagc gagaccgcat gatctgtccc aagcaataag
     8941 aggtcccaag gatatacata cgccacacat agcgcaaata gtacatacca aaggctgcaa
     9001 gtagcaagta aaataggcta agaaggaggt cctgctgggt taattgcccc gatgtgatgg
     9061 catcaatgac ccgccccata accataggag gaatgagatt gagaacggaa accaagacca
     9121 gggccacaat cccgactaga taacggcgtt tttctaactt gaaaaaccac caaagttttt
     9181 gaataatgga cataaaatcc ctttctgatt gcaaatggaa acctgaggcc aatactcaat
     9241 gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgc tcaaagcact gctttgaggt
     9301 tgtagataga actgacgaag tcagtaacct acatacggca aggcgacgtt gacgcggttt
     9361 gaagagattt tcgaagagta caagacccca ggtttttctt tttcataagt tacgactgta
     9421 acagcaccct tgtcatattc agcaataaag atattggcta cattgtcatg cccttgttta
     9481 ctgaggttat caagcaacca ctcttcgcta cgaccaatcg attccaagac atctacttga
     9541 atcacaccgt cagtcacaac tggatactta ggattttcat ctcccatttg cacaacgata
     9601 agttgcccat tttgctcttg cacagctcgt ttgacttgct tcatctggaa aatcccttgg
     9661 ctacgaagtt tgagggctac atccgatgca gacaaaccaa ctgaacgaca ggcttctggg
     9721 tcaatattcc catttttgat aaggagagtt ggcttaccat caatcaaacg tttcacaaag
     9781 tgaacattgt tgttgagcca tttgagagtc aagaccagtt tgattaaaat ttcgatataa
     9841 ttaagtgtca tttgttcaca tccaccagtt caattgcgtc tgtcttatac aattcgactt
     9901 tctctaacag gtacttgtct ggctcacttc cgtttagggc acgataatag cgatctccca
     9961 ccttgataag tgcgccatct gtgtttgtgg aagtattaac atagacttct gacttgtcta
    10021 ctcccaaatc tttggaaaca acctcgatga aatgaagtga agtttgaaat ttattgttag
    10081 aaacttgatt gttttgataa gtcgtgatgc tgaccaaaag cagagctaaa taaggccaga
    10141 gctgaaatca tgaccaactc acgaaattcg gttccctttt tatcatggta tgccttgaaa
    10201 gcaaaaaaaa ctgtcacagc taatagaaca atcccaaagc caaccatgat accattttgc
    10261 tgaccgattt ggctgagtac atagtcataa gagtagaatt tcatatattt tcactccctt
    10321 tcataaaatc attcttaatt ataaacgaaa gagagtaatt tttcaaacca tacgttgggg
    10381 aaatagatct ttttttggta taataaaatc tataatctga atgaaaaagg taactttatg
    10441 aaacttatct catggaatat tgattcccta aacgctgccc taactagtga ctcagctcgt
    10501 gccaaattgt cccaagaagt cctacaaacc ttggtcgctg aaaatgctga tatcattgct
    10561 atccaagaaa ccaagctttc tgccaaagga cctacaaaga aacacgtgga aattttagaa
    10621 gaactcttcc caggctacga aaacacatgg cgctcttccc aagagcctgc ccgtaaaggc
    10681 tatgctggaa ccatgttcct ttataagaaa gaacttacgc ctacgatcag ttttccagaa
    10741 atcggtgccc cttctaccat ggacttggaa ggccgtatca tcactctaga atttgatgca
    10801 tttttcgtaa cccaagttta cactccaaac gctggtgacg gtctcaaacg cttggaagaa
    10861 cgccaagtct gggatgttaa atatgctgag tatttggctg aactagacaa agaaaaacca
    10921 gtccttgcga ccggtgacta caacgtagcc cacaatgaaa tcgaccttgc aaatcctgct
    10981 agcaaccgcc gttcacctgg atttactgac gaggaacgtg ctggatttac caaccttttg
    11041 gcaactggat ttacagacac cttccgccat gttcatggcg atgttcctga acgctacact
    11101 tggtgggcac aacgcagcaa aacttctaaa atcaacaata caggctggag aatcgactac
    11161 tggctcacaa gtaaccgtat cgctgacaag gtcactaaat ctgatatgat tgactcaggt
    11221 gctcgccaag accatacacc gattgtcttg gaaattgact tgtaaggatt ttctatatgg
    11281 actacaatgc ggtcattccc gagtttcttg tatcgaatat cgaacagtcc cggtccttct
    11341 actgtggttt actgggtttt aggattgaat actaacgtcc agaagaaaac tttctctttc
    11401 tcttgaagag tgtcaactaa tgttagagga gggaacaaag gatcaactag ctgaactgac
    11461 ctaccccttt ggtcgtggag tcaatctctc ctttggcata aaggatgttc caaaactcta
    11521 tcaaaaagtg atggaggcaa actatcctat ttatcgtctc ttgaccaaaa gaaagtttcg
    11581 tgtcagtgat ccctatatct atcctcataa atttgcagtt ttggatccag atggctattt
    11641 tttaagattt agcgagtaga ataataaaaa ttgggaaaag tctttaaagt tagaaaaagg
    11701 catattgtca ggtgtctaaa aacttgatag tatgcctttt atgatggaaa aatcttctaa
    11761 tactcttcga aaatctcttc aagccacgtc aacgtcgcct taccgtaggt atatgatact
    11821 gactctgtca attctatcca caacctcaaa acagtgtttt gaggttgtgg atagcttcct
    11881 agtttgctct ttgattttca tttagtataa atgttagctt acaatgaagt ttttgttcac
    11941 cctctcttct ttttgttcgc tttttaaacc aaaacactaa caaaatagtt gatttctaaa
    12001 gttatttagt gtaaaataaa aacattggct caagcctatt taaaattgaa tatcgtttta
    12061 cttgtttatg tcgtgaattg gcacgacgtt tctacaaggt gccggaacac ctaacaataa
    12121 gtaagtcagc agtgagatat ggccgttttt gccatgtcta tttaggggac ttacttagag
    12181 attaagtagg tcttttttga atatttttaa tatttccctt aattagtgcg gacgggaggt
    12241 gaaattatcc tgtgaataat ttcaggaatc cagggaattt catccctaat ttttgagccc
    12301 ctagctcttt catttctagg cttaggctaa aatagttccc cgaactattt tactctcaaa
    12361 aatcccgtca tagacaataa caagattatt gtctatgata ccactacaac gggaaatatt
    12421 tgataagact cgcttcgctc gtaatagttt tatcaaatat atagtattag caaaagggat
    12481 cactatagaa atatttaaac aacaagtaaa ttaactttta gactttagga ggtcttatga
    12541 aattattaga agagcgcatc ctcaaggatg ggcatatctt gggtgataac atcctcaagg
    12601 tagattcctt tttaacccac caagttgact ttagcttgat gcgagagatt ggtaaggttt
    12661 ttgcggaaaa atttgctgct actggcatta ccaaggtcgt aaccattgaa gcgtcgggta
    12721 ttgccccagc cgtttttaca gctgaagcct taaacgttcc catgattttc gccaaaaaag
    12781 ctaagaacat caccatgaac gaaggcatct taactgctca agtctactcc tttaccaagc
    12841 aggtgaccag cactgtttct atcgctggaa aattcctctc accagaggac aaggttttga
    12901 ttatcgacga tttccttgct aatggccaag ctgctaaagg cttgattcaa atcatcgaac
    12961 aggccggtgc cacagtccaa gctatcggta tcgtgattga gaaatccttc caagatggcc
    13021 gtgatttgct tgaaaaagca ggctaccctg tcctatcact tgctcgcttg gatcgttttg
    13081 aaaatggtca ggtcgtattt aaggaggcag atctctaatg caaactcaag aaaaacactc
    13141 gcaagcagcc gttctgggct tgcagcactt actagccatg tactcaggat ctatcctggt
    13201 tcccatcatg attgcgacag cccttggcta ttcagctgag cagttgacct acctgatttc
    13261 tacagatatc ttcatgtgtg gggtggcaac cttcctccaa ctccaactca acaaatactt
    13321 tgggattgga ctcccagtcg ttcttggagt tgcattccag tcggtcgctc ccttgattat
    13381 gattgggcaa agccatggta gtggcgctat gtttggtgcc cttatcgcat ctgggattta
    13441 cgtggttctt gtttcaggca tcttctcaaa agtagccaat ctcttcccat ctatcgtaac
    13501 aggatctgtt attaccacga ttggtttaac cttgatccct gtcgctattg gaaatatggg
    13561 aaataacgtt ccagagccaa ctggtcaaag tctcttgctt gcagctatta ctgttctgat
    13621 tatcctcttg atcaacatct ttaccaaagg atttatcaag tctatctcta ttttgattgg
    13681 tctggttgtt ggaactgcca ttgctgctac tatgggcttg gtggacttct ctcctgttgc
    13741 ggtagctcca cttgtccatg tcccaactcc actctacttt gggatgccaa cctttgaaat
    13801 ctcatctatt gtcatgatgt gtatcatcgc aacggtgtct atggttgagt caactggtgt
    13861 ttatctagcc ttgtctgata tcacaaaaga tccaatcgac agcacgcgcc ttcgcaacgg
    13921 ttaccgcgca gaaggtttgg ccgtacttct cggaggaatc tttaacacct tcccttacac
    13981 cggattttca caaaacgttg gtttggttaa attgtcaggc atcaaaaaac gcctgccaat
    14041 ctactacgca gctggtttcc tggttctcct tggactgctt cctaagtttg gcgcccttgc
    14101 ccaaatcatt ccaagctccg tcctcggtgg tgccatgctg gtaatgtttg gttttgtatc
    14161 aattcaaggg atgcaaatcc tcgcccgtgt tgactttgct aacaatgaac acaacttcct
    14221 tatcgcagct gtttcaatcg ctgcaggtgt cggtctcaac aacagtaatc tctttgtcag
    14281 catgccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtagcca gcctactcgc
    14341 tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg tgcgagcctc
    14401 ttttttgttg acaaaataaa aacatctacc tattcatata tgctagtgtc atagtcctga
    14461 aaatcatctg catcaagaac aatttcacaa ctcttatact tttctcttac aagtcgttcg
    14521 gcttcatctg gattttcagc ctctatactt actaaacgtg ataaagtttc tgtaatttct
    14581 actgtatact ttttcagttt tagattgctt tccaataatt cacctattaa caataatcta
    14641 tgataggagc tatgtagcca ctctggcaat ttatctccta cttcccaccc atatttttcc
    14701 ttcatcaaag ctagttgttt ttcgtcacca aatttcatat tttcagataa ccatctcaca
    14761 caatcgggat ctgataaggt acagacgatt aaacttaggt tatcgtaacg gctttcagtt
    14821 tcataatcag gccagatgac ttttaagaaa tcatacacct cttctgatgc aacaagttgt
    14881 ttcgttttca taatttccga tactttcctc tacctttaaa ttctattatt tctttgtctc
    14941 ttaatatttg aagctgttgc ctaatctttt ctttgatatg attgttctta acaaagatat
    15001 tttttaggtc actttcaaaa cgatacatat cttcaagggt aaattctgaa ccctctatct
    15061 tatctataca atttagaatt tctattgtcc aacctcttga tgacagagag ctcttcctta
    15121 aaaataaacc ttgcttaaat tcttttgtaa ctttttctgg atctctaact tgtccatctt
    15181 gcacaagaaa tatccttcct ttagaaggta cttgtgataa atcaatgtta caaccaatcc
    15241 aacctgctcg tctagcagtt ggtgcaagtg gttttctttg aataatcgat ttcggtgtaa
    15301 caaattgctt cggaaggaca agaaagttat ttacctcaaa attttttgtg taagttaaaa
    15361 aaaagaaatt aggattatta tctgcctgca cacgcttcat catcgttgca taagcaccat
    15421 cattgattgt tgatgaaaaa tttccttttt tgctctttag ttcaaactcc tcactacaat
    15481 gattacagta aaaatctgct acaggccgat tattttcaaa atgatttaag ggattgttcc
    15541 cacaattagg gtaataagac tgacgataaa cccaatcctc cgttaagata cgtgcctttt
    15601 ggctattact tttataggtt tctactaatt ctaaattaaa gtgtaattcc atttaaaaat
    15661 tcctcactaa tatgagaact cactcgatat aagcaattaa aatctactat aactagtata
    15721 ccacatatat gtacctatta catttgtatt tcatcactct cttttatatt tccttctaac
    15781 atatctcata aattaaaaat gagtgagata aatttatccc actcaccttt ctttatatcc
    15841 ttacccgtct ttcctcagac ctttggccta tgacaaacat ggtaaaggtt gctttgcaga
    15901 catttctgcc ctcttgattg gtgatatcca catccatcac acaggttgta cggccttgat
    15961 ggacacattc tcctttaatg gtcaatacat cgtcgagttt tcctgcctta aggtagttga
    16021 tagaggattg gagtgtcacc ccatcaagtc ccagcgagat aaccaccaaa ccactgattt
    16081 ggtcacatag cgtgaataga tagccaccat gggcattgcc atagtagttg agcgacgagt
    16141 tcactacttt ggtcgtcacc acaacgtgac catctctcat tttttcaatt tcgtaatttt
    16201 caaaggcaga tatagcgtca aaatgaaagt ctttcatcgt ttcctcctga tatttcaaac
    16261 gacactatga tactactttt tataggactg tgcaaggaga aagctcctag tctagtaaaa
    16321 ttccgacatg ttccacaaag cgcataaagg ctgtctgccc ttgttctgtc tgcttgtaga
    16381 ctcctgcatc ctcaagtaca cgcgcaaaga tagcacccac agagtccttg acgattgcaa
    16441 gggctttttc tttatccgtt aggtctggat gttgggattt gagttggtct gcccactcct
    16501 gatgataatc ggcaactgta acagcttctc ctacaagata gctcgcgact tgctccactt
    16561 cttctttcag acgtggtggc aagattgcca agcccatgac ctcaatcaag ccgatatttt
    16621 ccttcttgat atgttggaca tccttgtggg gatgatagat accatcagga tactctgctg
    16681 aagtctgatt gtctcgcaag accaagtcca actcaaactg tccatcgcgt ttgcgggcaa
    16741 tgggtgtgat agtgtgatgc ggtgtcctgt ctgtctctgc caaaatctgc actgcaggat
    16801 ctgaatactg gcgccattcc tgcaaaatct tatcagccaa attgatcaaa tcctctttgg
    16861 aatccgaagt caaacgtagg acagacatgg gccacttgac aattccagcc ttgacctgct
    16921 caaaaccagc aaatcggaag gccttttgca agggagccaa ttccatagga aatacgtgac
    16981 ggcctccctg ataatgatca tgagttagaa tagagccccc cacaatcggc aggtcggcat
    17041 tagatccagc aaaatatcct ggaaactggt ctacgatagc caacagacgt tcaaaactct
    17101 gacgactaat ggccatggga cgatgctggc catctaaaaa gatacaatgc tcattaaagt
    17161 aagcataggg cgaatactgg aaaccccatt cctgaccaac catttcaaaa cggataatac
    17221 ggtgattgct acgagctggg tggttaactc gaccatggta gccctcattc tctagacaaa
    17281 gctgacactg aggataatta ctagcttgca ccaacttggc tgccacaatc tctttcggat
    17341 ccttttcagg cttagagaga ttgatggtaa tttcaagttc tccataatca gatggaacac
    17401 gataagcgat atttttagca atggccttga gtttgatgta gtcatttttc tgactgagtt
    17461 gataaaaatc ctctatcgct tgctcaggat agtggacata ggtttcccag aagtcacgat
    17521 tgacctgact cggacaagga gtcaccaagt ccatcagttc agtaccaagg atttcacgcg
    17581 cagtctgact atcctcaatc gtctctaatc gaacggcttc ctcaactagc tggtccttga
    17641 ggtcaatcaa tttatccaga ttggtctcaa cttccaaaac accttctccc actcgtgcca
    17701 aaacacgatt ggtcaggtag attcgatcca tttcctcaaa tgaactttca gaaatgacat
    17761 gtgttacaaa tttatctact aaggtcacca tagagcctcc ttttgactag tcaaggacgt
    17821 gagtgccacc tgcaacttca gcgatataga agcttggagc gtatccaact acttcctcgt
    17881 agtgtttgcc tacagtttcc ttaaaggcct caacagtatc tttttgaacc aaggcaatgg
    17941 cacagccacc aaaaccagcc cctgtcatac gagcaccgag aactccttct tgtgcccaag
    18001 ctgtgtgaac aagagtatcc aattccaaac cagttacttc ataatcatgc tccagagaaa
    18061 cgtgtgacgc attcatcaaa cgaccaaatg tttccaaatc acctgcttga agggctgctt
    18121 gagctttaag ggtacgttgg ttttcaagca cagcatggcg agcacgtttc aaacgatttt
    18181 catctttaat caaatagcta tattggtcaa cggcccactc gtccaattca cccagggtct
    18241 gaatatccaa ggcaacttgc aattcttcca ctgctttttc acactcagca cgacgttcat
    18301 tgtatttaga gtccgccaat tcacggcgtt tgttggtgtt catgataaca acgacattgt
    18361 ccttcaaatc aagtggcacc aaatcatact ctaaggtgtt ggtatcaagg taaatagcac
    18421 gttgatcggc ccccatacca atagcaaact ggtccatgat accagagttg actccgataa
    18481 agttgttttc tgtttgtttt ccgattttaa ccaaatcgag acgctctaat tttaaatcaa
    18541 agagatgctc tgccacgact cctgtcaaga gctccaagga tgctgaagaa gacaagccag
    18601 caccatttgg gatattccca taaacataaa aatcaaaacc tttgtcaatc acgtgcccag
    18661 cttcttgcaa gaaatgaagg acaccttttg gataattggt ccagttgtgc tctttttcaa
    18721 acttgaggtc agcgagaggc acttcgataa tgcccttgtc ctcaaagtta gctgagtaga
    18781 aacgcaagac ttggtcgtca cgcttacgag ctgcaccgta agttcccaag gaaatagcag
    18841 caggaaaaac gtgcccaccg ttgtagtctg tgtgttcacc aatcaaatta atacggcctg
    18901 gtgaaaagaa ggtttggtct gcttcttgac caaaaacagc aagaaagtct ttgcgaagga
    18961 cttcagtagt aagatgttgt gccatatgaa ttctcctttg actgtccgtt aagccatctt
    19021 aacgtgtaat cgttttcttt ctattgtatc caaggaattt cccaaggtca atccttttta
    19081 ctaaaatttt actaaaatat cagtaaaaag aagaaaaata tgatatacta acctaatcaa
    19141 ggaggattta tggctacctt aaaagacatt gcacagctag cctctgtctc aatcgcgacc
    19201 gtatcccgtg tcctcaaccg cgaccagagc ctatctgtta cagaagaaac cagacaccgt
    19261 attttaaccg ttgctgaaga gctaggctac accaaacacc tcaagacagg cgagtcccac
    19321 aagcccaagc aaaagattgc cattatccaa tgggtcagcg aacaagggga gctagacgac
    19381 ctctactact accagattcg cctaggaata gaaaaaagag cccaagagtt ggactatgat
    19441 atcttgcgct attttaatga ccatcctttt accctaagcg aggaagtgat tgggattctc
    19501 tgcatcggaa aatttagccg agctcagatt tctgcctttg aagaatacca aaagcccctg
    19561 gtctttctag acagtgatac actttccctg gggcatactt gtattattac ggatttttac
    19621 actgctatga aacaggttgt cgattatttc ctcagtcaag ggatggaccg catcgggatt
    19681 ctaacagggc ttgaggaaac aacagaccaa gaagaaatca ttcaggacaa gcgtctagaa
    19741 aacttcaaaa actacagtca agcgagggga atctatcatg atgaactggt ctttcaagga
    19801 agatttactg cccagtctgg ctatgactta atgaaggagg ccattcagag cttgggagac
    19861 caacttccgc cagcattttt cgcagccagc gatagtttag ctatcggtgc cctccgtgcc
    19921 ctccaagaag ctggaatcag cctgccagat cgcgtcagcc tcatttcatt caacgacacc
    19981 agtctgacca aacaggtcta tcctcccctc tctagtatta cagtttatac tgaagaaatg
    20041 gggcgagcag gtatggatat tcttaacaag gaagtcctcc acggtcgcaa aattcctagc
    20101 ctgaccatgc tgggaaccag actgacatta agagaaagta ccctaaatca agaataggat
    20161 aacataaaaa acgaatagag ttctaaaact cctattcgtt ttttattcga ttacaatcat
    20221 agacttaatt gtcttacgtt catccatatc tttataggct tggtcgatat cttccagttt
    20281 ataacttgaa gtaaagacgc gacctggatt gatatcacca tcaaggacgg cttttagtaa
    20341 aaattgctta tcgtatgttg tagcagaagc tgccccacct gctacagaga tattttgcat
    20401 aaatgtcgaa ccaagagcac gattattata gtgtgggact cctacaaagc ccatacgccc
    20461 tccattatga agaacaccta gcgcctgttc tatagcagcc tccgtaccaa cacattcaag
    20521 tgctgcgtct gctcctccgc cgaggatttc acgcaccttg gtaattcctt cttgaccacg
    20581 ttctgcaaca acagctgtcg cacctgactc catagccatc ttttgacggt cttcatgacg
    20641 gctcataagg ataatttgtg atgctccacg catcttagcc gcgatgacag cacattgacc
    20701 aacagcccca tcaccgataa caacaacctt gtcccctttt tgaacatttg caacacgcgc
    20761 tgcatgatag cctgtcggca tgacatctgc aagagtcaaa agggacttga gcatcccttc
    20821 tgtataatca gaaggttgac cagggatttt aaccagcgcc cagtttgcat agtggaagcg
    20881 aatatattct gcctgaaaat caccccccaa attattacca atatgattgt cgcaggaacc
    20941 gtcaaatcca gcaagacagg catcacactc accacatcca tgtgtaaaag ggacaatcac
    21001 aaaatcacct ggtttcaccg tcgtaatggc ttccccagct tcttcaacaa tcccaatcgc
    21061 ttcgtgtcca ctatttttat gtccagcttt cgtttctgga ttacggtacc tccataaatc
    21121 tgaaccacaa acgcacgcac gaaccacacg aataatcaca tcatccgctt ctattatttg
    21181 cggacgttca atgctagcaa gtccaacctg acctgccttt gtatatactg ctgatttcat
    21241 ttaaaatttt ccttccttat aaagtttaat tttgagattt aaacgattta aagctgtctg
    21301 tagctgagac aagcgctcct ctaatttttg cttttcctct tctaaaatac caagcctctc
    21361 ctctctcgtt tcatctccct tttggtagag cgacatatag tcaactaaac tatctacaga
    21421 gacacccgcc gaacgaaaac acttaataaa ttccagcgct tcgatatcct gatcttgaaa
    21481 atcacgaatc ccagtagcag tacgagtaat cggtggcaca agaccaaccc gttcataata
    21541 ccgaatcgta tccgctgaaa ttcccaacaa atcactggca gatttaatat tcacacgcta
    21601 acctcctctc atttcattca ctgaattcac gctattctaa ctcacttatc ttattatata
    21661 actttgagtt gactccaagt caagcatttt gtatctaatc gaaaaatttt tatcattaac
    21721 aatcatatat ctggagttta tacaatgctc ttctcttacc aatttttgaa atatttaaat
    21781 aataaagaaa ttactctatt ccaaataaga tagtacccaa gtatttttca cttttttcta
    21841 atgttgatgc tcataactcc gttccaagtc acacaccttt cgcttatggg tttggtgagt
    21901 ttctaggtca gcatcaattt caatggtaat attttgaaaa ccacaatctt ttaggaaaat
    21961 tcgaatagac tctttacaag tttccatatg ttccatttct tttagacaaa catgtacaat
    22021 ggcatttttt tccaaagcat ccatagtcca gagattaagc tgattaaggc tggccacatt
    22081 gtccaatcgc tccaggccac tctttacttg cttgatatca agaccttctg gcacagcatc
    22141 caagaaaatc ttgagtgtag accaaaaacg tggaagggct tttgaaagaa taaagaaaga
    22201 aatgacaagg gacaaaagag gatctaggat ataccagtcc gtaaatcgaa gaacaatcgc
    22261 catcaggata acagctaccc accctagcgt atcttccaga aaatgcaaac tcagaataga
    22321 ctcattcttt gtctttccct taccaaccac cagactcgct aacagattga tagtaatcgc
    22381 aataattcct aaccagagaa tcccctcatc attgactggt tgcggatgca aaatcttcgt
    22441 gacattttcc aaaatgacta gaacagagcc cgttacgaga atcacagctg ttaccaaggc
    22501 tcctagcagg ctaaaccgct tatagcccaa ggtgtactga ttgtcttctt cacgattgga
    22561 gattgtttct agaaaagctg atattccaat tgcaatcgca tctcccaagt catgcacaga
    22621 gtcagcaaga acagcgctag aaccaaatac tccacctgca ataaactcaa caatggcata
    22681 agtcaaattt aagaaaaaag ccacccaaac agcatatttt gccttcatat ttctcattcc
    22741 tttgttataa tagatttatg aacaccttgt tcattatcat tatccactaa aactcaaaga
    22801 aaggatagaa gcaaaacgtc agctttattc agttctgaac aatttgcctt aagtgtccat
    22861 atgactaaca ttgaccgccg tatcagcaaa accaaaaaag ccatctatca agcttttata
    22921 caacttttga atgctaaggg ctacgaggcc actactgttc aggatatcat tgatctcgca
    22981 gatgtgggaa gatccacctt ttactgtcac tatgaaagta aggagcttct tctggaccag
    23041 ctctgccgct acctcttcca tcatcttttt gaaagagagc aagccatttc aaccgaggac
    23101 tacctcgccc acctctttct ccattttcag aaaaaccaag accatatcac cagtctgcta
    23161 ttttccaaaa atgactactt cctccgtcaa ctccataaag agctagaaca ccatgtctat
    23221 tccgtgctag ctgataattt aaaagaagcc caccctaata tgcctacttc ttacctccaa
    23281 cacttggtcg tgtccaactt tatcgagaca ttgacctggt ggctcaaaaa aggtcaagat
    23341 ttcataggcc aggaagttgt ccaattttat ctagaccttc tcattcctaa aaattgaata
    23401 tagagtaaag ctcagttgtc ttatttctag gttactgagt tttttatctt ttcaacaaca
    23461 aaagaggacc cgccgatcct ctttttcata ctataaatcc ttgattatca actatatctg
    23521 ttttaatcga aatctcaaaa cagcactttc aaacatcttt tcctagttaa gtaaatcagt
    23581 attctgctta gctgccttgc tccattgata ccaaccaact agactgttaa tgagataaat
    23641 tagatatttc ccttgaattt gcaggctttc tccccaccag agatagattg aaaaaacatt
    23701 ggtagccgcc cagaatatcc actgttcacg gtaaacagct gtcatgagga tttgccctac
    23761 cccattggtt gcatctgtga ttgaatcacg atagggacga ttggcaccaa tagactgata
    23821 aatgaagcca aaggccaacc accaaagcac actaatggaa agatactttg tccagccctt
    23881 gccgtccagt ttacgcgcga caaactcctg cttttccttc ttaaactgtg cctgataaat
    23941 ccaaactaga agtccaattg gctgcatgac tgtgaagtaa agtgtcgtca gcacctcacc
    24001 ataaaagcct ttctgtaggg ccaaaataag gtaaataaca gagttaatca agccaaaaag
    24061 ataattactt gctcgacctt ccgatacaaa gattacacag ataatccctg tcaagctaca
    24121 aatcatccca atccagtcaa caatacgatg ttcgtaaacc aactccagcc agagaggaaa
    24181 acttcctaaa accagcaaat aaatccactg ggcaaaacta cgatgggcaa agaggtcatc
    24241 ccagatagcc ttcatagttc ctgaaaatcc taaatcagcc atagccgcaa ccatacgacg
    24301 gtaaccacct gacatttcac ctagggttgt tttgatattt tcaattttct tttgcaaata
    24361 agtatgcatc atttctcctt ttgtttttaa agagccgtgt ctggatagac tttcggacgc
    24421 aacgctctat tagataatga actgcctata cacaagattt ctaaccttag tcgacatgag
    24481 ctaaaacctc ttatttgtta agtagttcac aaaatattat acacctattt tatgaatagt
    24541 caactgtctt tacagtaaaa ttttagaaaa tcatgaaaat tttctctttc tttccatttt
    24601 aagtgacatt cagtcattct cacatcaaaa aagcccagac gaaattgtct gagcattctt
    24661 ttatctagtc gtttaaggaa gttgagttca gtatgtttaa agtctctgtc ccatcatttc
    24721 ttcaacaaac cttgttcttg gagaaactcc ttggctactt gctttgctga cttgccttca
    24781 acaccgactt ggtagttgag ctggctcatc tggctttctg taatcttacc agccaatgta
    24841 ttaagaactc tttccaactc tgggtgtttc ttgagaagag cttctttcat gagtggagcc
    24901 ccttgataag gtgggaagag ttgcttgtca tcttccaaga cctgtaaatc ataacgctcc
    24961 aattccgcat cagtcgaata ggcatccgtg atttgaatat cccctgactg aattgcctga
    25021 taacgaagag ctggctgcat agtcgccaca ttgagattga gaccatacat tgattgcaag
    25081 cccttatttc catcttcacg gtcgttaaac tcaagcgtaa agcctgcctt cagctgccct
    25141 tccacttttt tcaaatccga aatggtcttc aagccatatt cttgcgcaat ctttttcgga
    25201 acagctacag cataggtatt ttgataagac ataggtttga gataggctag atgatcctgc
    25261 ttggcaatgc tatcacgcgc cacttgataa acctgctctg gctcatgact caccttgggt
    25321 gatggttgaa gcaaactttc agtcaccgta ccggtaaatt caggataaat gtcaatatca
    25381 ccttttttca gagcttcata aaggaaactt gtcgtcccaa aattcggttt aacagtcgca
    25441 gtcatgctgg tattttcttc aatcagcaat ttatacatat tagccaaaat ttctggttct
    25501 gggcctattt tcccagcaat aatcaagttt tccttctctt tttgagccaa aagggctgga
    25561 ctataagaca gacccagtaa taaagccacc aaggcaaaac cagagaaaat cgtccgcaat
    25621 ttcgcttttt ccattacttt tagtaggaag ttaaaggcaa tagctagcac tgcagaagaa
    25681 agggccccaa tcaaaatcaa actggcatta ttacggtcaa ttcccaacag gataaaggaa
    25741 cccagtcccc ctgccccaat caaggtcgcc aaggtagccg taccgataat caagaccgct
    25801 gccgtccgaa ttccagacat gataacaggc atggcaagtg gaatttcaaa cttcttgaga
    25861 cgctcccatc tggtcatccc aaaggcaatc ccagcctctt gaagactcgg atcaattccc
    25921 ttcagcccag taatagtatt ttgtaaaatc ggaaaaatcg cataaatcac tagagctgtc
    25981 aatgccggta aagtcccaat tcccatcaag ggaataaaga gtcccaacaa ggccagagac
    26041 gggatggtct ggaaaatccc tgcaatctgt aagacccaat ccgccaactt ctcatgatag
    26101 cgaagaaaaa cagccaaggg aatcgcaagc aaaatagcta gtaacaaggt caaaagtgac
    26161 aactgcaaat gttgagatag agctgtcaac caatcactaa aacgatcctg aaaagttgca
    26221 attaaattag tcatgaacac tacctccaaa caagtctgct acaaagtctg ttgcaggcgc
    26281 ttttaaaatt gtctcgggat tcgctacctg gcgaatttct ccatcctgca agacagcaat
    26341 acggtccgcc aacttcaagg cttcatccgt atcatgggtt acaaaaatcg ttgtcatccc
    26401 aaactcttta tgcaattctt ttgtcagaac ctgcaactgt tttctcgaaa tagcatccaa
    26461 ggccgaaaag ggttcatcca tgaggaaaat cttgggctga ccaatcatag ctcggacaat
    26521 accgacccgt tgctgttctc caccagataa ttcactaggt aagcgatgcc catactcggc
    26581 tactggtaaa ccaaccttag ccaaaagctc ttctgttttc ttcgtaattt cttccttgct
    26641 ccaccccttc atttcaggaa tgagagcaat attttccgca actgttagat ttggaaaaag
    26701 agcaatagcc tgtaaaacat aaccagtaga aagacgaagt tcacgctcat catagtcttt
    26761 gatgcgcttc ccatccatat aaatatttcc atcagttggt tccaaaagac ggttaatcat
    26821 cttgagcatg gtcgtcttac ctgacccaga aggccctact aaaaccataa attccccatc
    26881 ctcaatctgt aagttgacat ctctcaagac atccttttct gtgtagcata gtgctacatt
    26941 tttgtattca atcattcttt gtcctcattt aaaacttccc tcgattggtc aagtcttcta
    27001 ccttaggcat gacttcctta ttatcccaat gctccacaat tttcccgttc tttaaacgga
    27061 agatatcgta ctgggcataa gcaacgccat caatctgagt ctgaccatag ctaaccacat
    27121 agtttccttg tcctaagagt tggaaaacaa agtcaaaagt gacactatat tcagccacat
    27181 agtttttata agcagcactt ccttgtccaa tatcatgatt atgctgaatc aaatcgtctg
    27241 ccacataatc actccactgc tctagctccc cattttggaa aatttctgtc aagaaacggc
    27301 gaaccagctt tttattttct gctttcttgt ccaaatcctt gatttcaaaa tctccaaaaa
    27361 tttgatctag ttggtcattt tcaggtgttc gatagtagtc aatgacatcc caatgctcaa
    27421 caatacgacc attctcatcc gcacgaaaag tatccgtcgt cacccattga gcttctccac
    27481 cattcagata ttgatgaaca tgaacaaaga ccagattgcc atcctcaatg gtgcggacaa
    27541 tcttaatctg acgctctgga tgacgctcaa agaaatctgc aaagaaggct gcaaatcctt
    27601 ctttcccgtc aggaacacct gtcgaatgtt ggatataggt agcccctaca gactgggctt
    27661 gagcctcagc aactcgtccg tcttgaatgg catggatgta taggttgtga gcatttttca
    27721 cttgttgtga catattctaa acctcatttc ccttctcttt cagattcgcc aaaattcttt
    27781 cttgaaaacc ttcaaattgg tgaatttctt cctctgaaaa tcctttgtaa aagatagtat
    27841 ccaatttctg actgacacga tgccccactt ctttctggga cttgcctaac tccgttaaaa
    27901 ctaaatactt cttacgcttg tcttttccac acggactaac aattacaagc ttttgttcct
    27961 ctagcttttt tatcatagtc gtcagcgtat tattcgcaag tccagtcgca agcgcgatat
    28021 ctgtcgcagt tgcgcagcca gtttcactat tccataaaac cgctaaaatc ttgccctgtt
    28081 cacccctata aagagcctca ggatcttgac tcagtaactt ttgaaaaatc cgcccattca
    28141 acaaacgaat atgatgggct agcaaatgac catctttcat aacacctcca atttatttcg
    28201 atatcgaaat gaataaaaca attgtaacac tcatcgttct aactgtcaac tatttcgatt
    28261 tagaaataat ttttgataat tatccacacc accatactcc ggctcaacta acttttaacg
    28321 agagtttcta aactccttcg tcctccagtc tacaaaagcc ttccatccgt actatcctat
    28381 attttatgag gggacacatt tttcctatca gaccatttat tttaaagata gaagtaaatc
    28441 ataattgctt ccatctgttc ttttatagta tattgaagtt ggactagggc actgtatctt
    28501 ctaaaacatt gatagaaagc gatttgaatt tcccaatcaa tttgttcgtc tttatagcat
    28561 ttcgaaactg gaataggaca ccatgactgc taaaagattt ctataaattc atttgatttc
    28621 ctcaatcaat ttgttcatat cttatttcat tccgctataa tttcacctta ccctatcttt
    28681 ttcgtagcac ccttcaaaca gcctatcccc taccgtttga cgattcctca cttcactcca
    28741 cttccattac agaagtttct tcactactat ggtctcggct gacttctcat gattccttgt
    28801 tactactatt tgaacgctca cgagataggt cttataaaaa atgctttgat ccacaatgga
    28861 atcaaagcat tttaaaaagt tcctcataca taagtgcaga agtcgcagtt cctctgtact
    28921 tggcttcttc tcttttgata aagcgagcca agttgagcaa ctcaggtgct ggatgtttgg
    28981 gatttaggag caattcacga ttgaccaggc ctgagagacg aactgcctgc aattgctcat
    29041 ttgtagtagg cagtttttta gtagtctcta ggagagcagc aactaaatct tcactcaaat
    29101 catgtcgagc atgattgtaa agatctttta taaggctttc taggtttggt tctgccatcc
    29161 ctaccacctc ccttatggtt taataatgtt taatcaaatc aaccgttgaa cgatccaatt
    29221 tcttcaccaa ggcttgtaag aaagcttgcg cttctaggaa gtcatccatt gcatagaggg
    29281 tttggtgaga atggatataa cgagcgcaga caccgatagt tgttgatggg acaccaccat
    29341 ttttcagatg agctgcacct gcatctgttc cgcctttacc acagtagtat tggtacttga
    29401 taccagcttc ttcagccgtt gtcaaaagga aatccttcat ccctgggaga agcaagtgac
    29461 ctggatcata gaaacgaatc aaggttccat ctccaatctt gccttgacca ccgtagacat
    29521 cacctgctgg tgagcaatca actgcgagga agacttctgg gtcaaacttg gttgtagagg
    29581 tatgagcgcc acgcagacca acttcttctt ggacgttaga acccagatag agttcattgc
    29641 cgagtttttg acctaataaa gcttcagcta gctcgcttac catgaggaca ccgtagcggt
    29701 tatcccaagc ttttgagatg atattttttt cattggctgt caaaattgca gaactatctg
    29761 gtacgatggt atcaccagga cggatgccaa aactttctgc ctcagccttg tccgcaaaac
    29821 caccatcaaa aacgatatcg gcaatggctg gcatggttgg tccccccttt ccacgagtca
    29881 aatgcggagg aacagaacct gaaatcacag gaatttcatg accatcacga gtcaagagtt
    29941 tgaaacgttg gctgctaacc accatggggt tccagccacc gatttctacg acacggaagg
    30001 taccatctgg cttgatttcg ctgaccataa aaccaacttc gtccatatga gaagcgacca
    30061 agacgcgcgg tgcatccaca gcttctgaat gtttgatacc aaaaatacca cccaagccat
    30121 ctgtcaccac ttcatccaca tgcggtgtca acttttcacg aagataagca cggacaggcg
    30181 cttcatgacc tgagactgca gcaagttctg ttacttcttt aatttttgaa aataatgttg
    30241 tcatttcagt tccttctttc tttcatccat tttaccactt tttataggag aaggatagtg
    30301 ggaaggtgga tttctaagtt agtatcttag tcctgctcta tcttagaaaa ggatagtatt
    30361 ctcttgcatg tagtgcaaaa tctagtaaac attccaaaat taactcgaat atttatttcc
    30421 aaacaaaaaa acaatacacc atcaaagttg tttggatttt tcatgaaatt tacagaaaat
    30481 agttgacttc cctttcttct ttctttaaat atatagttgg ttgagtttgg aatagtacgc
    30541 tgtagctgct aaaacatttc tagaaattaa tttgactttt ctaatagagt tgttcatatc
    30601 ttatttcaat ttactatagt acaaaactag aaaaggaaaa aatcatgacc aaaacaattc
    30661 ttgttacagg tgggactagc tacattggct cccataccgt taaagctctt ttaaatgctg
    30721 gctatcaggt gcatgtcctc gataatctct ccacaggaaa tcgagcggct gtggatagtc
    30781 gtgctagctt taaagaactg gatgtttatg atgctagcgc cttaaaggct tacttagaag
    30841 aaaatcagat tgatgctgtc ctccattgtg caggtgaaat tgttgtgagc gaaagtattg
    30901 aaaatccaag taaatacttc actgccaacg ttgctggtat ggaccaagtt ctcaaagtct
    30961 tatctgaagt tggcattcaa aaaatcatgt tctcttcgac tgcttctctc tatggaaata
    31021 actgtattga caagccggtg actgaagata ccctgctcga ccctgtcaac ccttatgcag
    31081 agacaaaact gatgggcgaa cgaatgattt actggatggc caatcgctac gactggaaat
    31141 atgttatttt ccgttacttt aatgttgctg gggctgaaat ggatgcttca aacggtctgc
    31201 gtgtgaaaaa tccaactcac atcattccaa atatcaacaa gaccgcattg ggacaacatg
    31261 atagcttaaa aatatttgga gatgactacg atacacgtga cggttcatgt attcgagatt
    31321 acatttatgt cttggacctt gcataggctc atgttaaagg aatgaactac ctatttcaag
    31381 aagacagttc ttctcaaatc tttaacttag gaactaaaaa aggctatact atcaaagaaa
    31441 tctttaaaac tgctgaagaa ttactaaatt aaaaaatccc acacgagatt gttgctcgac
    31501 gtgctagtga cccagccagt gtcctagcag atgcttcaaa agctaaacaa tatcttgatt
    31561 ggacggctag ctactccctc gaagatatta ttttatcaga ttaaggtaaa aacattttag
    31621 aataggataa caaaggagaa agtcttgttg ctctctcctt gttgtattta ttaaattgtc
    31681 agaaatttta ttgacagatt agaaaaataa tgttacattt atatccgcag gtatctttcg
    31741 ataccaaatc tacatgaaag gacggggtat gaaactttct cattatttaa ttggcttact
    31801 tctactccta gtctttctct ctattagcat tgggaccagt gatttttcat ggggaaagct
    31861 atttgatttc gaccagcaga cctggctcct ctttcaagag tcccgtctcc caagaactat
    31921 cagtattctc ctgactgcct ctagtatgag tatggcaggc cttctcatgc agactattac
    31981 ccaaaatcag tttgctgcac cgagtacagt tggaacgact gaagccgcca aactgggaat
    32041 ggtgctgagc ctttttgtct ttccatcggc tagtctgacc caaaagatgc tcttcgcttt
    32101 tatttcatcc atcgtattca ccctcttctt cctagccttt atgaccattt ttactgtaaa
    32161 ggaaaggtgg atgttgcctc tgattgggat catctatagc gggattatcg gctcagtcac
    32221 agaagttatc gcctatcgtt tcaatctggt tcagagtatg actgcctgga cccagggctc
    32281 cttctccatg attcagaccc atcagtatga gtggctcttc ttaggcctca tcatcctgat
    32341 aaccgtttgg aaattatccc aaaccttcac catcatgaat ctagggaaag aaaccagcga
    32401 gagtttgggg atttcctact ccctacttga aaaactggcc ctctttctgg tggcgctaac
    32461 gacaagcgtc accatgatta ccgtgggtgg cttaccattt ctcggagtta tcgttcccaa
    32521 tcttgttcgc aagcgctatg gagataatct aagtcaaacc aaactcatgg tcgcactggt
    32581 tggtgccaat ctagttctgg cttgcgatat cctatcccga gttctgatta ggccctatga
    32641 gttgtctgtc agtctcttgc taggaatcat cggtagtctc gtctttatcc tacttctctg
    32701 gagaggggga cgaaaagatg cagactaaaa gcaaacatac caagctcttc tggattctca
    32761 ttattcttgc catcggagct tgtcttctct acttttggcc catcactcac ttgtcagcct
    32821 ttgcttggaa gttgcgttcc caaaagatca tcgtttatct cttggtagcc atcgcgactg
    32881 ggatttcgac cattagtttt caaaccctga cggaaaatcg cttcctgacg cctagtattt
    32941 tgggaattga atccttctac gtcctactac aaaccctact actggttttt gaaagcaagt
    33001 ttcttcaact tggcaaatcc cctatcttag aattcctagt cttacttctt gtccagtccc
    33061 tcttctttct cgccttacaa ggttacttga agacactgat gaagcaagac ctggtcttca
    33121 tcctgctgat ctgtctagcg ctcagaagtc tctttcgaaa tatcagcacc ttccttcaag
    33181 tcctaatgga tccaaacgaa tacgataaac tgcaaaatag tctttttgcc tcctttcaac
    33241 atcttaacac ttccatccta gccatcggtt ctctgatcat cctcgctttg acaatctttt
    33301 tctttcgaaa agcagtcgtt ctagatgtct tgcacctgca aagagaaacg gctcagatat
    33361 tgggactcga tgttgaaaaa gaacagaaag agctcctctg gggaatcgtg cttttgacct
    33421 caacggccac tgccttggta ggacctatgg ccttcttcgg ctttatgctg gccaacctca
    33481 cctacctgat tgtcaaagac tatcagcaca agttactctt tatagtggcc attctggttg
    33541 gatttattag cttaaccttg gggcaagcct tgattgaacg agtctttgca ctggaaattc
    33601 gtatcagtat gatcattgag agtgtgggtg gcttcttatt ctttatctta ctatatagga
    33661 gggctcgtca gtgaaactgg aaaacattga caaatccatt caaaaacagg atattttgca
    33721 aggcatttcg cttgaagtca gtcctcaaaa actgacagcc tttattggtc caaatggtgc
    33781 tggaaaatcg actctcctct ccatcatgag cagactaacc aagaaagatc agggagttct
    33841 cagtatcaaa ggacgtgaaa tcgagagctg gaattcgcaa gaactggccc aagaactaac
    33901 catcctaaaa cagaaaatca attaccaagc caaattgact gttgaagaac tggtcagttt
    33961 tggacgtttt ccctacagcc gaggtcgact tagatcagaa gactgggaaa aaatccgaga
    34021 aactctgaac tatttggaac tgaccaactt aaaagaccgc tacatcaata gcctgtcagg
    34081 ggggcaactc cagcgcgtct ttatcgctat ggtactggcc caggatacgg actttatctt
    34141 gctggacgaa ccactcaaca atctcgatat caagcaaagc gtcagcatga tgcagattct
    34201 tcgacgactg gtggaggaac tcggcaagac cattatcatc gtcctccacg atatcaacat
    34261 ggccagtcag tatgcagatg aaattgtcgc cttcaaggac ggccaggtct ttagcaaggg
    34321 aagaaccgat caaatcatgc aggctgacct gctcagtcaa ctttatgaga ttcccatcac
    34381 gctagctgat atcaataaca aaaagatctg tatctatagc tagtaacata aaagctcaag
    34441 ttagagaacc ttcagtctct tagtcaataa gattaagaga ctccctaaat cgttatcaca
    34501 ttttaaaaag gagaaattat gaaaacatcc cttaaacttt atttcactgc cctagtggcc
    34561 agcttcttgc tcctacttgg tgcatgtagt acaaactcaa gcactagtca gacagagacc
    34621 agtagctctg ctccaacaga ggtaaccatt aaaagttcac tggacgaggt caaactttcc
    34681 aaagttcctg aaaagattgt gacctttgac ctcggcgctg cggatactat tcgcgcttta
    34741 ggatttgaaa aaaatatcgt cggaatgcct acaaaaactg ttccgactta tctaaaagac
    34801 ctagtgggaa ctgtcaaaaa tgttggttct atgaaagaac ctgatttaga agctatcgcc
    34861 gcccttgagc ctgatttgat tatcgcttca ccacgtacac aaaaatttgt agacaaattc
    34921 aaagaaatcg ccccaaccgt tctcttccaa gcaagcaagg atgactactg gacttctacc
    34981 aaggctaata tcgaatcctt agcaagtgcc ttcggcgaaa ctagtacaca aaaagccaag
    35041 gaagaattgg ccaagctaga caagagcatc caagaagtcg ctactaaaaa tgaaagctct
    35101 gacaaaaaag cccttgcgat cctccttaat gaaggaaaaa tggcagcctt tggtgccaaa
    35161 tctcgtttct ctttcttgta ccaaaccttg aaattcaaac caactgatac aaaatttgaa
    35221 gactcacgcc acggacaaga agtcagcttt gaaagtgtca aagaaatcaa ccctgacatc
    35281 ctctttgtca tcaaccgtac ccttgccatc ggtggggaca actctagcaa cgacggtgtc
    35341 ctagaaaatg cccttatcgc tgaaacacct gctgctaaaa atggtaagat tatccaacta
    35401 acaccagacc tctggtatct aagcggaggc ggacttgaat caacaaaact catgattgaa
    35461 gacatacaaa aagctttgaa ataagccact tcaaactcaa tttaatactc aatgaaaatc
    35521 aaagagcaaa ctaggaaact agccgcaggt tgctcaaaac actgttttga ggttgcagat
    35581 gaaagctgat gcggtttgaa gagattttcg aagagtataa aattttgaca ttctaaaacg
    35641 catcctatca agttcactca aacctgatag gatgcgtttt tatcattttg aaatctattt
    35701 accccacctc attccccttc ttgatttcgt ttaagggaaa agtggtctgg aacggggtcc
    35761 ttacctgttt tcgaccaggg atgacaacgt aaaatccgag ccaagcccat caataccccc
    35821 ttaaaaccat gtttttcaat agcctgaatc atgtagttgg aacaagtcag ctcaaagcga
    35881 caagagggtg gaaagactgg tgagataaaa cgttggtaaa agcgcacaag cgctattaaa
    35941 attcgtttca ttatttctta gttacagcca tggtgtgtag ttggctgatt tcttttttat
    36001 taagacgacg ggattctcct ggacggagtc ctgtcaagtc taggtgtccg aaacgagtcc
    36061 gagacaactt atctacttgg agaccaacag cttcaaacat ctttttaacc tgatggttac
    36121 gcccttcatg gatggtcaac tgcaccacag agcgattttt gactgggtcc actttgagaa
    36181 tttcataaac agctggcttg gttttcttac catcaatctc aagaccacgg gtcaaggggc
    36241 gaagattgtc cttattggcc acacctttaa cacgcgcgac ataaaccttg tcaatctcat
    36301 tacgagggtg aatcatctcg tctgtaaagt ccccatcatt ggtcaaaatc aagactcctg
    36361 atgtatccca gtccaaacga cccacagggt aaatgcgctc tttgacattg ggcaagaggt
    36421 cgacaaccgt cttgcgaccc ttgtcatctg ttacactgga aatgacaccg cgtggtttgt
    36481 taagcagata atagaccttt tcttcgttgt agataggttg accttcaact tcgaccttgt
    36541 cgcctgactt gatagtggtt gctagttcac gcaccacttg gccgttaact gtcaccaaac
    36601 cttgcttgat cagctcttct gcttttctcc tactggccac acctgcgtgg gcaatatact
    36661 tattgattct catcttcttc tatcctttca ccaaataatt ggctttcttg ggcttgaatc
    36721 tcaagctcat caatcactgg taattcttct aaatggttta tccccatgta atctaggaaa
    36781 taatccgtag tcacatagag gttggggcgc cccaatactt cctttttccc gtcttccttt
    36841 atcaggtcca aagcttgcaa ctttgccaag gctccactcg agttaacccc acggatggca
    36901 tcaatttcta tccgcgtaat cggctgtttg taggcaataa tggacaaggt ctcaagggca
    36961 gcccgagaca agctctggtt gataggcgcc ttagagtatt ccttcaaaat ctctgcaaat
    37021 tgaggcttgg tcaccaatct ataagcacca cttgtctcaa tcaaagccaa actggaatct
    37081 gggtcctttt catacttctg ggctaatttt cctaaacttt gctggatgcc tgtcggtggc
    37141 agagagagga gttcagctaa ctggcggacc cgaatcccat cttcacccgc tacaaacaag
    37201 agcgcttcta tttttgctaa agtactcatc tttcctctct atcaagtcta gctttgaggc
    37261 acttgacttt cttccttctt ttccatgaga tagatatctc caaaactctc ctcttgcacg
    37321 aggatcagct cctgggtttt gattaactct agggttgcca aaaagagggt gatgacctct
    37381 tggacattct gggcttcctt gaacaaatcc tgcaagcgca attgatctcg tccaatcaag
    37441 gattctttca caataatcat catgtcctca atcttatact catcccgcaa gatagtcgtg
    37501 tgattttgtg caaactcctc ttttttcttg gctaggatat ttgaaaaagc caaaaagagg
    37561 tcaatggtcg tcttgtcatg cacaagctcc gcatcttcgt aaatcaactc tgtcggcgct
    37621 ttggaataat actgggcccg ttcttggtgc ttggcttcca agtgctcacc caagagcttg
    37681 aacttgcgat attcttcgat ttgagagagg aggtcctgct ccaggtcatc ccccaagtct
    37741 gtcacttctg ctaccttcgg aaggagttta cgactcttaa tcagcatgag ctgactagcc
    37801 atgaccatgt actcacccgt cacttccaga cgcatggcct gcagggttga gacataggct
    37861 agatactgtt cgatgacttc cgtaatgggc acatcgtaga tatccatctg gtacttagaa
    37921 accagatgca agagtaagtc caggggtcct tcaaaatctt ttaatttaat atccattatc
    37981 tatatttttc taaggtcagg actgttttta atcctaattt ttttgcaatt tcgtacaaat
    38041 cgaccttgtt ttctatttgt cttagaataa actgttcacg taagacttga gctgataagg
    38101 atggaaaacc tttctccttg acaaaagatt ctaactgacg aaaggcccac tgacgagaat
    38161 agggtttccc tcctctttca aaaagatagg tctgccccat caagggttcc aattctgaaa
    38221 gcaaggccgt gggaatggtg acaatcctcc gttgggaagc cttgctgatt cgcaacacct
    38281 gaaaatccag attgatgtcc gcaaccttga tggctaaaat ctcactgggc aagagcccca
    38341 tttctaggat taagagcgct agcaagcggc cctctggatg gtcgctttcc tgccaaaaag
    38401 agtctaggta tagaatctct ggcttttccg tcttcttttc agcttgtttg gctaattcca
    38461 agcggtaaaa gctgtccacc tctccttttt gatagagaaa gtatagaaat tggttacagg
    38521 ccgaaatctt tcgcttctgg gcgctgattt ttagattggc tagctgggct tggtaaatct
    38581 tgagactggt ctcagaaatc cgctcaccta ccatgtctaa aaattgctcc aaatcatact
    38641 tataggactg cttggaattg acagacaagc cctgcttttc ctctaaaaag gctgaaatcc
    38701 tgtctctcat tggcatcgaa tctcatattc cttactaaag tcatgcaaga gggcattaac
    38761 ggccttgagg atggacttgc gcgtaataat cccttggaaa ataccctctg catccacaac
    38821 cggtaagaag gactcatcta ctagcttgtg caagacctcc gtaatggtga aatcaggcga
    38881 aacaaccgct acgtccgttt ttgtcatatg aacgatatcc gtatccgcca tgatttcttg
    38941 gctcaagtca tgctccatct gataagccat aatatctctg agtccaatcg tcccaacaaa
    39001 ctgtttttca tctgtcacaa cgggaacacg ggtataggtc atctgactga gcaagagggt
    39061 cgcatgatcc gcattgtgag tatcaatcaa cacagctaga tttttagcag gggtcaaaaa
    39121 agtttcctcc tgccccaaca agaaagtctc aaactccttg gcaatcatcg gctaaactcc
    39181 ttggacaaac ctggatacac ctcgtgatct cgtgtcaaaa agtccacttt gaagtaacta
    39241 tcatcaatct ccacacgagc atagagacat tctctgatgg tacctcgtgg ttgactgata
    39301 gagcctggat ttagaaagag gatcttgcct tccaaccaag cacttggcac atgcaagtga
    39361 ccatagaggc agatagcggc ctcttcctcc tgagcccagt agtccaactt ttgaaagttg
    39421 aaattgatgt caaacaagtg accatgagtt tggataatct tggtcgaacc aagctcagtc
    39481 accagacgtt ctgggtagcc ggcgtagaag tccatgttcc ctttaacaac gcggatgccc
    39541 tcccaaagtg gagaatccgg acgtagttca gaatcgccgt tatgaaaaac ggcatcgact
    39601 ttgcccacat agcgatcacg gacttcttcc acaatcaagc tatcgccatg ggaatcgctc
    39661 attacaatga tggtttgctt tgccatgatg gaaatacctc caaaagtttc ttaacggcta
    39721 aggcacggtg agattgacta tttttttctt ccagggttaa ttcagctgat gactcacctg
    39781 tctctcctac aaggaagagg ggatcatagc caaagccatt ttcaccctta ggttcaaagt
    39841 taatataacc tgaccagtct gcttcaacaa ctaaactttc cttatttggg ctggctacga
    39901 ctagggttgt gtggaactga gccgagcggt ccttgagttc aaagaccatg gccaattcgt
    39961 gcaagagttt ggcattattt tcacggtcag ttgctcccac acctgcgaaa cgagctgacc
    40021 agacgcctgg taagccacca aggacatcga ctttgagacc agaatcatct gccaaaacca
    40081 tcttgcccgt taattgagaa atggtttctg ccttaaggcg ggcattttcc tcaaaggtca
    40141 tacctgtttc tgctacttca ggcaggtcag ggtagtcatt aagattttcc acatcgtagc
    40201 ctaacttatc aaagatagct cggaattcct tggttttacc ttcgttacga gtcgcaatca
    40261 acaaggtttc tctgactttg cttgtctcaa agaaatcatg aacattgacc ccttctttag
    40321 gcaattctac atacaagagt tgcccatttt caaccaagag cagggcccca tgacgttgaa
    40381 taacttccaa gtttcgtccc atttcttgat taagcatatc taccacaaag gagaacaaac
    40441 ggtaggtaga accatagcgt aaaacagtaa ctgaaagcgg gaaaccatac tcttcatctc
    40501 caaatatttt ggagaattca aacagaggaa aatctgtctg atagtcgctc aaactgttaa
    40561 cgccaccaaa aatactataa gacccaacat accagtcctg gtcatcctta tattcataaa
    40621 ttttatttgt cataattcta catgctccac atgaatctct ttttccagcc attcttcacc
    40681 aatttgtgca aaactttggc tactggctgt tgtgtaaaaa cggtgatgga gtggtccagc
    40741 atcgcgacca cgattgattt caaaataatt gagtaagact gagatatccc gtacgcactc
    40801 tgccccacta tcgatgagct gaacctttgg ccccatcaca ttttggataa tagggcgaag
    40861 gagtggataa tgagtacagc ccaaaatcag gctatccacc tttccaacca agggacgcag
    40921 ggtttcatag accaccttct tggtaacact ggttgacagg gcacctgact caaccaaggg
    40981 agcaaacttg ggacaggcca agctctccac ctgtaagtcg ggatccagat catggatttt
    41041 ctgacggtat atgtctgatt gtaccgtcat gggcgttcca atcactccga ttttcccacc
    41101 ttgactggac ttgatggctg ccgaagctcc tggcaaaatt acacccaaga caggaatatc
    41161 tagttgagcc ttgatttctt cccagacgac cgcagtcgca gtgttacaag caatgacaat
    41221 cattttgaca tccttggtca agagaaagtt gaccagctgc caagtatatt cacgaatttg
    41281 ctcagcagga cgggggccat agggcgcccg cgccgaatct ccaatataga cgatttcttc
    41341 atggggaagc tggcgcatga gctcgcgcac aacggtcaag cccccgacac ccgaatccaa
    41401 aaaaccaatt ggtcgattat ccatacagtc ttctttctag tcttttttct gattgatcgt
    41461 tcattatgat tccttgtact gaacgaactg accgacagct tgatagaatg tcaactacct
    41521 ctctcttaat cataatcaaa tatcaataga atgcaaggaa aaagtctcat ccctaaacca
    41581 taaccaacat agtgagaagt ctggaacttt catcccagac ttttccttat ttattttttc
    41641 tttttattgt tagcaagggc tgccttttgt tggcggatga tttggtggta aacttgttgt
    41701 accttagctt cgcttggttt ttgaccattt gcactcaaaa gagtacgaac tgcttcagca
    41761 ttcaaacgtg ggttgtcagc aaattctttt tcgatttgct tacgaaccaa gtacattcct
    41821 ccaagagctc ctcctagaaa agctagcaca atcaatacaa ttgctaaaag taaatccatc
    41881 atttttctcc tgtttctttt tgaatccctt tcattatacc acaaactagc caccctgacc
    41941 agtttgttct acactttcag ggatggaata gacagcaaaa agaaccctgc ttttctgaaa
    42001 aagaggtctc cttgctaagt ttatactcaa tgaaaatcaa agagcaaact aggaagctag
    42061 ccacaggttg ctcaaagcac cgctttgagg ttgcagataa aactgacacg gtttgaagag
    42121 attttcgaag agtattaatt tacataaata gccagtgttt gatagggttt gagtagaatt
    42181 ttctcagaca cttctgcatc ttcatagttt gatatcaaaa tctgtccatt ttggtagact
    42241 gctggcaagt cgatttccac ttctttagca taaaagttat tgagcactag taacttttga
    42301 tcctcaaact ggcgttcaaa agcgtagact tgtttgctat cttcaaaggc tggtttgtaa
    42361 cttccttctg aaatgattgg catttcctta cgcagtcgaa tcaagtcttg atagaaggtg
    42421 aaaatcggac cctggatttc attttctaca ttgatgtatt tataggattt accagctttc
    42481 aaccatggag tgcctgttga aaatcctgca ttttccgaag catcccactg catgggaatg
    42541 cgtgaattat cacgcgactt agcttgaata atctggaagg cttcttgctg actctttcct
    42601 tcttctaaga gcgtctgata ggcattaagc gattcgacat ccacataatc agccatagaa
    42661 tcatagtctg ggtcaatcat cccgatttct tcacccatat agatataagg tgtcccacgt
    42721 gacaggtgaa tgctagccgc tagcatggtg gctccttcct tgcggaagtt ttgaatatcg
    42781 acaaaacggt tcaaggcacg tggttgatcg tgattgttcc aaaagagggc actccaaccg
    42841 tctttatcac tcatttcctt accccaacta tggtaaagac tcttcaactc ttcaaaatca
    42901 aagggagcca aggtccactt ttgtccatcc ttatagtcca ccttgaggtg atgaaaatta
    42961 aaggtcatgg ataattcctg acgatcaggc gacgaataga ggacacagtt ttccatggtg
    43021 gtagaagaca tttccccaac tgtcataaag ctatcgtcag atccaaaagt ggcttggttc
    43081 atcatacgca aatagttatg aacgatgggt ttgtctgtat aagctggctt cccttcattt
    43141 tcaggacagt ccactgaaac ctcgtcctta ccgatcaaat tgatcacatc aaatcggaaa
    43201 cctttgatac ccttgtcgcg ccagaaatta acaaccttga aaagctcctt acggacattg
    43261 gaattgcgcc agttaaggtc agcctgggtc tcatcaaata ggtgaagata gtatttccca
    43321 gtatccccga aaggcgtcca tgcagaacca ccaaacttag actgccaatc tgttggttgg
    43381 tcttggatga agaaaaagtc ttgataatac ttatcaccag ctagggcttt ctgaaaccat
    43441 tcatgctctg tcgaacaatg attaagtacc atgtccagca taaagtcaat cttgtgctct
    43501 ttaccgacac acaccatttc ctcaaaatca gccatatcac caaaaagagg atccactgcc
    43561 atataatctg aaatatcgta accattatcc cgttgagggc ttggatagaa tggattgagc
    43621 cagaccatat ccacacctag tttggctaaa tagggaattt tttcgataat cccacggaaa
    43681 tccccaatac cgttttcagt ggtgtctttg taagattttg gatagatttg atagactact
    43741 tttcctttat caagtgtcat ctgtttctcc ttttctgata aaagggagga agcagtcttc
    43801 cgtccctatt tgtgctattt caattatact caatgaaaat caaagaacaa actaggaagc
    43861 tagccacagg ttgctcaaaa cactattttg aggttgcaga tagagctgac gtggtttgaa
    43921 gagattttcg aagagtatta gattcgtgta gcgaccatga gagatgctcc agcttggatc
    43981 gttgtcggat aagttccggg aatagtcgct gtataagcat cttggttggt gatgataaca
    44041 ggagtttctg tcaccagacc tgcagcctta atgacatcca tatcaaaacg aatcagttgc
    44101 tgaccaactg taacgtgatc tccttggact acaagacttt caaaaccttt gccatcaaga
    44161 cctactgtat ccataccgat gtggatgagc aattcaactc cctcgtcaga gacaatgccg
    44221 atggcatgct tggtagggaa aagaaccgtc actgtcccat taactggaga ggtcaactca
    44281 ccttggcttg gttcaatgac tagaccttgc cccatgacac ctgatgcaaa aataggatcc
    44341 gtcgcttgac tcaattcttt cacttggcca gttagtgggc tgataatttc taccgaagta
    44401 agttctactg gttcatggtt cacaaattct gcttcttctt gagcaacgaa ttctgcctgc
    44461 aagttcgtat cgccctctgt ttttgtaaag agaccagcct tgcggaagaa gaaagtcaag
    44521 agcattggaa caacaatcgc aactagcata gttcctgcaa atggcagcat gtattgaggt
    44581 tgaatagaga gaatacctgg caaaccaccg ataccaatag aagccgcagt tacattaaaa
    44641 gtaacggata acatgcctgc aagggctgaa ccagtcatcc cagcaacaaa tggataaata
    44701 tattttacgt taaccccaaa aagagctggt tctgtaacac cgagataggc tgaaatggtt
    44761 gcaggaagtg aaacctgagc ctcacgctca tcatggcgat gcatgaaata atagacaaac
    44821 acggctgagc cttgagcaat attagaaaga gcaatcattg gccatagggc agtgccacca
    44881 gcatccgcaa tcaattgtgt atcaatggca ttggtcatat ggtgcagacc tgtgatgaca
    44941 aatggagcgt agagggcacc aaaaattgca ccgaagagcc atttaactgg accagttaaa
    45001 cctgccaaga caactgatga aagtccttgt ccaattgtcc aaccgattgg tcccaaaaca
    45061 gtatgagcca aaatcaaggc tggaatcaat gacaagaaag gtacaaaaat catagaaatg
    45121 acttctggga tatgcttgcg ccagaagatt tcaagataag acagactcaa acctgcaagc
    45181 aaggctggga taacttgggc ttggtaaccg atacgattaa cagtaaaata gccaaaattc
    45241 caaacccagt ttgccgcgat atcagctgct ggcgttgaag caaccgcata ggcattgagc
    45301 aactgaggcg ataccaaaca gattccgaga acaattccca aaatttggct ggttcccatc
    45361 ttacgagaaa cagaccaagt aatccctact ggtaagaact ggaagatagc ttcaccaggc
    45421 aaccagagga agtgattgac acctgcccaa aactgagagg attctgtgat ggtcttgcca
    45481 tccaacatcg accaatggac accttccaag acattacgga aaccgaggat caatcctccg
    45541 actatcaagg ctggaataat cggagtaaaa atctccgcca gagtggtcat aacacgttgg
    45601 accacgtttt gattactctt agctgcagac ttggctgctt ctttggaaac accctcaata
    45661 cctgaaacgg ctgtaaaatc attataaaag atgggcacgt catttccaat gattacctga
    45721 aattgacctg catttgtaaa agttccttta acagctggaa ttgactcgat agctttaaca
    45781 ttagccttct tatcatctcc taaaacaaag cgcatccgtg tcgcacagtg ggtgacggca
    45841 gtcacatttt ctttgcctcc aattgcctga agcagatctt tggcttcttg ttcaaatttt
    45901 cccatttgat tctccttata cttgtcaaag caagtttgat ttgataagta tattgtaaac
    45961 gataccaaac ttgtctgcaa cttgtttggt ataaaaaggg tgtttttttg taaattttct
    46021 agcacttgta tggtaaaata gatacaggtg ttcattaaac tagactaaaa acctatttaa
    46081 gcaggcaaaa tgaagaaata ccaacaatta tttaagcaaa tccaagaaac cattcaaaac
    46141 gagacttacg ctgtcggaga tttccttcct agcgagcacg accttatgga gcaatatcaa
    46201 gtgagtcgtg ataccgtccg aaaggccctg tctctcctcc aagaggaagg attgatcaaa
    46261 aagataagag ggcaaggttc tcaagtcgtc aaagaagaaa ccgtcaattt ccctgtatcc
    46321 aacctaacca gctaccaaga actagttaaa gaacttggac tgcgctctaa aaccaacgtg
    46381 gtcagtctgg acaagattat tattgataaa aaatcctcac tgataaccgg tttcccagag
    46441 tttcggatgg tttggaaggt ggtccgccag cgtgtggtgg atgatctggt atccgttctg
    46501 gatacagact atctggatat ggaactaatt ccaaatctca ctcgccaaat tgctgagcag
    46561 tctatctatt cttatataga aaatggcctc aaactcctta ttgattatgc tcagaaggaa
    46621 atcaccattg accactcaag cgaccgagac aagattctca tggacattgg caaagaccct
    46681 tatgtcgttt cgattaaatc aaaagtctat ctccaagacg gacgccaatt tcagtttacc
    46741 gaaagtcgcc ataagttaga aaaatttaga tttgtagatt ttgcaaaacg caagaaataa
    46801 aagactgaga caccagatct cagccttttt cggctctata atatttgtag tgggtaaccc
    46861 ccctatggat attatggagc ctattttgtg tagaaaaaaa gtcccatatg acctataatg
    46921 aaaagcgaca aaacaactca ttagaaagaa tcatatggaa caattacatt ttatcacaaa
    46981 attactagac attaaagacc ctaatatcca gattttagac atcatcaaga aggatacaca
    47041 caaggaaatc atcgccaaac tggactatga agctccatct tgtcctgagt gcggaagtca
    47101 aatgaagaaa tattactttc aaaaaccttc taaaattcct tatcttgaaa caactggtat
    47161 gcctactaga attctcctta gaaagcgtcg tttcaagtgc tatcattgtt ctaaaattat
    47221 ggt
//
LOCUS       DAVKMU010000012        45065 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKMU010000012 DAVKMU010000000
VERSION     DAVKMU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45065
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.012"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(79..3756)
                     /gene="rpoC"
                     /locus_tag="VW343_000746"
     CDS             complement(79..3756)
                     /gene="rpoC"
                     /locus_tag="VW343_000746"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9755340.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(3789..7400)
                     /gene="rpoB"
                     /locus_tag="VW343_000747"
     CDS             complement(3789..7400)
                     /gene="rpoB"
                     /locus_tag="VW343_000747"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9755341.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            7816..7886
                     /locus_tag="VW343_000748"
     tRNA            7816..7886
                     /locus_tag="VW343_000748"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7848..7850,aa:Cys,seq:gca)
     gene            complement(8084..9415)
                     /locus_tag="VW343_000749"
     CDS             complement(8084..9415)
                     /locus_tag="VW343_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9755342.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(9644..10468)
                     /gene="endA"
                     /locus_tag="VW343_000750"
     CDS             complement(9644..10468)
                     /gene="endA"
                     /locus_tag="VW343_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9755343.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(10507..10695)
                     /locus_tag="VW343_000751"
     CDS             complement(10507..10695)
                     /locus_tag="VW343_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9755344.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(10688..11971)
                     /gene="murA"
                     /locus_tag="VW343_000752"
     CDS             complement(10688..11971)
                     /gene="murA"
                     /locus_tag="VW343_000752"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9755345.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(11995..12225)
                     /locus_tag="VW343_000753"
     CDS             complement(11995..12225)
                     /locus_tag="VW343_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9755346.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(12283..13320)
                     /locus_tag="VW343_000754"
     CDS             complement(12283..13320)
                     /locus_tag="VW343_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9755347.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(13304..13792)
                     /gene="coaD"
                     /locus_tag="VW343_000755"
     CDS             complement(13304..13792)
                     /gene="coaD"
                     /locus_tag="VW343_000755"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9755348.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(13782..14321)
                     /gene="rsmD"
                     /locus_tag="VW343_000756"
     CDS             complement(13782..14321)
                     /gene="rsmD"
                     /locus_tag="VW343_000756"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9755349.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(14386..15378)
                     /locus_tag="VW343_000757"
     CDS             complement(14386..15378)
                     /locus_tag="VW343_000757"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9755350.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(15490..16173)
                     /locus_tag="VW343_000758"
     CDS             complement(15490..16173)
                     /locus_tag="VW343_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9755351.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(16229..16969)
                     /locus_tag="VW343_000759"
     CDS             complement(16229..16969)
                     /locus_tag="VW343_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9755352.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSPALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            17003..17281
                     /locus_tag="VW343_000760"
     CDS             17003..17281
                     /locus_tag="VW343_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9755353.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17359..18285
                     /gene="yidC"
                     /locus_tag="VW343_000761"
     CDS             17359..18285
                     /gene="yidC"
                     /locus_tag="VW343_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9755354.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVHEELAKNPSKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(18360..19154)
                     /gene="pflA"
                     /locus_tag="VW343_000762"
     CDS             complement(18360..19154)
                     /gene="pflA"
                     /locus_tag="VW343_000762"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9755355.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(19280..20530)
                     /locus_tag="VW343_000763"
     CDS             complement(19280..20530)
                     /locus_tag="VW343_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9755356.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(20597..21424)
                     /gene="purR"
                     /locus_tag="VW343_000764"
     CDS             complement(20597..21424)
                     /gene="purR"
                     /locus_tag="VW343_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9755357.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(21691..22632)
                     /locus_tag="VW343_000765"
     CDS             complement(21691..22632)
                     /locus_tag="VW343_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9755358.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(22622..23878)
                     /locus_tag="VW343_000766"
     CDS             complement(22622..23878)
                     /locus_tag="VW343_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9755359.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(23880..24542)
                     /locus_tag="VW343_000767"
     CDS             complement(23880..24542)
                     /locus_tag="VW343_000767"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9755360.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(24505..25161)
                     /locus_tag="VW343_000768"
     CDS             complement(24505..25161)
                     /locus_tag="VW343_000768"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9755361.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVANPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(25172..26050)
                     /gene="rsgA"
                     /locus_tag="VW343_000769"
     CDS             complement(25172..26050)
                     /gene="rsgA"
                     /locus_tag="VW343_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9755362.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFFNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETHKKVSKKIPK"
     gene            complement(26052..26924)
                     /gene="rsmA"
                     /locus_tag="VW343_000770"
     CDS             complement(26052..26924)
                     /gene="rsmA"
                     /locus_tag="VW343_000770"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9755363.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFACLADALKGQGL"
     gene            complement(27055..27363)
                     /locus_tag="VW343_000771"
     CDS             complement(27055..27363)
                     /locus_tag="VW343_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755364.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(27356..27997)
                     /locus_tag="VW343_000772"
     CDS             complement(27356..27997)
                     /locus_tag="VW343_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755365.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRMIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(27999..30029)
                     /locus_tag="VW343_000773"
     CDS             complement(27999..30029)
                     /locus_tag="VW343_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9755366.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREKVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSI
                     LGLGILWYQGALFMATVQLVIIALLLYGLILAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQYSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(30298..31197)
                     /locus_tag="VW343_000774"
     CDS             complement(30298..31197)
                     /locus_tag="VW343_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9755367.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(31269..31829)
                     /gene="rnmV"
                     /locus_tag="VW343_000775"
     CDS             complement(31269..31829)
                     /gene="rnmV"
                     /locus_tag="VW343_000775"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9755368.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMMVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSCKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(31829..32602)
                     /locus_tag="VW343_000776"
     CDS             complement(31829..32602)
                     /locus_tag="VW343_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9755369.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(32907..33800)
                     /locus_tag="VW343_000777"
     CDS             complement(32907..33800)
                     /locus_tag="VW343_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9755370.1"
                     /translation="MVAQDPKAQDSTKLTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(34084..34218)
                     /gene="rpmH"
                     /locus_tag="VW343_000778"
     CDS             complement(34084..34218)
                     /gene="rpmH"
                     /locus_tag="VW343_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9755371.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(34363..35577)
                     /locus_tag="VW343_000779"
     CDS             complement(34363..35577)
                     /locus_tag="VW343_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9755372.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(35733..36027)
                     /locus_tag="VW343_000780"
                     /pseudo
     CDS             complement(35733..36027)
                     /locus_tag="VW343_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36189..36641
                     /locus_tag="VW343_000781"
     CDS             36189..36641
                     /locus_tag="VW343_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9755373.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(36676..38064)
                     /locus_tag="VW343_000782"
     CDS             complement(36676..38064)
                     /locus_tag="VW343_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9755374.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            38130..39092
                     /locus_tag="VW343_000783"
     CDS             38130..39092
                     /locus_tag="VW343_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9755375.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKNYMEG"
     gene            complement(39121..39279)
                     /locus_tag="VW343_000784"
     CDS             complement(39121..39279)
                     /locus_tag="VW343_000784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755376.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHVFEVVDETDEVS
                     NHTYGKAT"
     gene            complement(39343..40353)
                     /gene="ccpA"
                     /locus_tag="VW343_000785"
     CDS             complement(39343..40353)
                     /gene="ccpA"
                     /locus_tag="VW343_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9755377.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(40778..41377)
                     /locus_tag="VW343_000786"
     CDS             complement(40778..41377)
                     /locus_tag="VW343_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9755378.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(41379..42476)
                     /locus_tag="VW343_000787"
     CDS             complement(41379..42476)
                     /locus_tag="VW343_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9755379.1"
                     /translation="MLERLKSIHYMFWVSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VEQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     EAMTSMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(42476..43213)
                     /locus_tag="VW343_000788"
     CDS             complement(42476..43213)
                     /locus_tag="VW343_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755380.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(43215..44099)
                     /locus_tag="VW343_000789"
     CDS             complement(43215..44099)
                     /locus_tag="VW343_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755381.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(44086..44280)
                     /locus_tag="VW343_000790"
     CDS             complement(44086..44280)
                     /locus_tag="VW343_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755382.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIHGALGFGFM
                     IFVVFPMGMEWLKGESHDGG"
     gene            complement(44277..44483)
                     /locus_tag="VW343_000791"
     CDS             complement(44277..44483)
                     /locus_tag="VW343_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755383.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        12879 a        10501 c         8552 g        13133 t
ORIGIN      
        1 tttaccacat ttcgtggaaa aatggagaaa gttttcagaa gagagaatga gagaaccctc
       61 gggttctctc attctctctt attctactgt ttcttccaca gtttcaacgg cagtatccac
      121 aactacttct gttgtttctt catttccttc ttcctctact ggaggattaa ggtattcttc
      181 ttcgttgaca gcatgtggtt caaggttacg gtaacgggcc ataccagtac cagctgggat
      241 gatcttaccg atgataacat tttctttaag tccaaggaga tggtctttct taccacggat
      301 agctgcgtca gtaaggacac gagttgtttc ctggaaggaa gccgctgaca agaaactgtt
      361 tgtttcaagt gaggctttgg taattcccat aaggactggg cgacctgtcg ctggaactcc
      421 acctgcgata aggacatctt tgttggcatc tgtaaagtca ttgatatcca tgagggtacc
      481 catgagaaga tctgtatcac ctggatccat gacacggact ttacggatca tttgacgaac
      541 cattacctcg atgtgtttgt caccgatttc taccccttgg ctacggtaaa ctttttgtac
      601 ttcaccgaga aggtacgttt caactgacaa gacatcacga actgcaagga gacgttttgg
      661 ttggatagaa ccttctgtca gagcagcacc acgcgctact tggcccccaa cttcgacacg
      721 catacgagct gtaaatggaa cgacatattc accttcgcca gtttcaccct taacaaagac
      781 tttcttggta cgagttgatg catcttcttc gatagcagta acttgtcctt taacctctgt
      841 aataaccgct tcccctttag gattgcgggc ttcaaagatt tcttggacac gaggaagacc
      901 ctgagtgata tcggtatttg aggcaacccc acctgtgtgg aaggtacgca ttgtaagctg
      961 tgtaccaggt tccccgatag attgggcagc gattgtacca actgcttcac caacttcaac
     1021 cgcatcacca gtcgccaagt tgataccgta acagtgacgg cagacaccgt gacgagtgtt
     1081 acatgtaaat acagaacgga tagtcacttc ttccacacca gcattgacaa tttcacgcgc
     1141 cttgtcttct gtaatcaatt catttggacc aataatcact gcaccagttt ctggatgttt
     1201 aacagttttc ttagtgtaac gaccgttgag acgctcttcg agagactcga tcatctcttt
     1261 tccttctgcg atagaacgga tcaagagacc acggtcagtt ccacagtcgt cctcacggat
     1321 gataacgtct tgggcaacgt cgaccaaacg acgagtcaag taacctgagt cggctgtctt
     1381 aagggccgta tcggtcatac ctttacgagc accgtgagtt gagaagaaca tttccaatac
     1441 cgacaaacct tcgcggaagt ttgaaaggat tggcaattcc atgatacgtc cattcggagc
     1501 agccatcaga ccacgcatac cggcaagctg tgagaagttt gagatgttac cacgggctcc
     1561 agagtccatc atcataacga ttgggttctt aggatcttga ttagcaatca agcgtttctc
     1621 aagtttttca cgggcagcac gccattcagc tgtaacagca ttgtaacgct cgtcgtctgt
     1681 gatcatacca cgacggaatt gtttggtgat ttgttcgaca cgtttgtgtg attcttcaat
     1741 gatttcagcc ttgtcatcaa cgactgggat atcggcaata cccactgtca atcctgcaag
     1801 agttgagtgg tggtaaccga ggttcttcat gcggtcaagt agggcagaag tttctgtcgt
     1861 acggaaacgt ttgaagattt cagcgatgat atttccgagg tttttcttct tgaatggagg
     1921 gttgagctca agattgctga tagcttcctt gatagctcca ccaagtggca agaagtattt
     1981 agctggaaca ccttctgtca agttggcatt gtttggttct tgcaagtatg gtagcccctc
     2041 tggcatgata tcgttgaaga gaattttacc aactgttgta agcaagacct tatgtctttg
     2101 ctcttctgtc caaggcttgt tgaggctgtc tgttgcgata ccaacacgtg agtggaggtg
     2161 aacataacca ttgcggtaag ccataaccgc ttcgtcacgg tctttgaaga ccattccttc
     2221 accttcgcga ccagcttctt ccatggtcaa gtagtagtta cccaaaacca tgtcctgaga
     2281 tggagtaact accggtttcc catctttcgg gttcaagatg tgctcagcag ctagcatgag
     2341 gatacgagct tctgcttgtg cttcttctga aagtggtacg tggatggcca tttggtcccc
     2401 gtcaaagtca gcattgtagg cttcacagac aagtgggtgc aagcgaagag ccttaccatc
     2461 aattaagact ggctcgaagg cttggatacc caaacggtga agggtcggtg cgcggttcaa
     2521 aagcactggg tgttctttaa tcacttcttc aaggatatcc cagatacgct catctccgcg
     2581 ttccaccaag cgtttagctg ctttgacgtt ttgcacgata tcacgggcaa cgatttcacg
     2641 catgacaaat ggtttaaaga gttcaatcgc catttcacgt ggcacaccac attggtacat
     2701 cttaagagtt ggaccaacgg cgataacgga acgtcctgag aagtcaacac gtttaccgag
     2761 caagttttga cggaagcgtc cttgtttacc tttaagcatg tggctcaatg atttcaatgg
     2821 acggctacct ggtcctgtga ttggacgacc acgacgacca ttgtcaatca aagcatcaac
     2881 tgcttcttga agcatacgct tctcattttg aacgatgata cctggtgcat ttaactcaag
     2941 caaacgagcc aaacggttgt tacggttgat aacacggcgg taaaggtcat tcaagtcaga
     3001 tgaggcaaaa cggccaccat ccaactgcaa cattggacga agatctggtg ggataaccgg
     3061 aaggatgtta agaatcatcc attcaggttt gtttccagac ttgtaaaagg catccaaaac
     3121 atccaaacga cggatggctt tgacacgttt ttgtccagta gctgttttca attcttcttt
     3181 gagttcagca atttcttttt caagatctac ttgcttcaaa aggtcttgga tggcttccgc
     3241 acccatcttg gcaacaaatg aaccataacc atattcacgc aagcgctctc ggtattcgcg
     3301 ctctgtcatg atagacttgt gctcaagtgg tgtatcctta ggatcaatca ccacataagc
     3361 cgcaaagtag ataacttcct cgagggcacg agggctcata tcaagggtca agcccatacg
     3421 gcttggaatc cccttgaagt accagatgtg agatacagga gctttcaatt cgatatgtcc
     3481 catacgctca cgacgaactt tcgtacgcgt tacttcaacc ccacagcggt cacaaacaat
     3541 tcctctgtaa cgaatgcgtt tgtacttacc acaagcacat tcccagtctt ttgtaggacc
     3601 aaagatcact tcatcaaaga gtccttcacg ttctggtttc aaggtacgat aattgattgt
     3661 ttcaggtttt ttgacttctc cataagacca tgaacggact ttacttggag aagctagggt
     3721 gatttgcata cttttaaaac gatttacatc aaccactatt tcttcccttt ctattctaag
     3781 tgaactgctt attcttgttc agcagcttct tctgttgctt ccgcttttgt tgctttctca
     3841 gcttcttcag cttcaaaggc tgctttagcc tcttgggctg ctttttcgcg ggctttttca
     3901 aggtcatcta cgtggatgac atcttcgtcc attccttcat ccaagtcgcg aagttccact
     3961 tcttggtcat cttcgtctag gacacgcatg tcaagaccaa gagattgcaa ttctttgaca
     4021 agaactcgga aggattctgg aacacctggt tttggaattg gtttgccttt tgtaatagct
     4081 tcataggctt tcaaacgtcc gttgatatcg tccgacttgt aagtcaagat ttcttgaagg
     4141 acatttgacg caccgtaggc ttcaagagcc caaacctcca tctcaccgaa acgttgtcca
     4201 ccaaactgag ctttacctcc gagtggttgt tgggtaacag ttgagtaagg tccgactgaa
     4261 cgcgcgtgca atttatcgtc aaccatgtgg tggagtttga tcatgtacat gactccaaca
     4321 gaaacacggt tatcaaatgg ttcacctgta cgtccatcgt aaaggattgt cttggcatcg
     4381 ctatccatac ctgcttcttt aacagttgac caaagatctt cagaacttgc tccatcaaag
     4441 actggtgtcg caatgtgaat accaagagta cgagctgcca taccaaggtg aagctccata
     4501 acctgaccga tattcatacg tgatggcacc ccaagtgggt tcaacatgat gtcgactgga
     4561 gttccgtctg gaaggtaagg catgtcttct acaggaacga tacgagagac aacccctttg
     4621 tttccatgac gtccggccat tttatctccg accttaatct tacgtttttg agcgatgtaa
     4681 acacgaacca acatgttaac acctgattgc aactcatctc catttacacg tgtaaagatc
     4741 ttaacatcac gaacgacacc atcggcaccg tgtggtacac gaagagaagt atcacgcact
     4801 tcacgagact tgtctccaaa gatagcgtgc aagagacgtt cttcagctga aagatctttc
     4861 tcacccttag gtgttacttt acctacaaga atatcacctt ctttaacctc agcaccaata
     4921 cggataatcc ccatttcgtc aaggtctttg agggcatctt caccaacgtt tggaatttcg
     4981 cgagtgattt cttcaggccc aagctttgta tcgcgcgttt ctgattcgta ttcttcaagg
     5041 tgaacagatg tgtagacatc gtccttcacc aagcgttcgc tcatgataac ggcatcctcg
     5101 aagttgtaac cttcccaagt catgtaggca acgattgggt tttgtccaag cgccatttct
     5161 ccattttcca tagaaggtcc gtcagcgatg aaatcgcctt tttcaacgac atcaccaact
     5221 tttacgagag tgcgttggtt gtaagcagta cctgagtttg aacgacggaa tttttggatg
     5281 tggtaaacat ccaatgaacc atcttcacga cgaacttcta ccttgtcagc atctgcgtaa
     5341 gtaactttac catcatactg agcaatcaca gccgcaccag aatcgtgggc tgcttggtat
     5401 tccataccag taccaacgta aggtgcctga ggattaatca atggcacagc ctgacgttgc
     5461 atattggctc ccatgagggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacctg ttttggtgaa acgtccatgt agtcaacaat attagctgga
     5581 tactcttggt tgaccccttg gtgacgtccc atgacaatct tctcagcaaa ggttccatct
     5641 tcattcagac gagagttagc ctgagctaca gtatattcat cttcttcatc agctgtcaac
     5701 caaacaattt cgttcgtgac aacacctgtt tcacggtcaa ccttacggta tggtgtttga
     5761 acaaaaccat atttgttcaa gtgtccgtaa gatgacaagt tattgatcaa accgatgtta
     5821 ggtccttcag gtgtttcgat tggacacata cgaccatagt gagtgtagtg cacgtcacgc
     5881 acttcatatc cggcacggtc acgagtcaaa ccaccaggtc ctaaggctga caaacggcgt
     5941 ttgtgagaca actcagaaag cgggttgtgt tggtccatga actgtgacaa ctgtgatgaa
     6001 ccaaagaatt ctttaactgc agctgttaca ggacggatat tgataatttg ttgtggtgtc
     6061 aagacttcat tgtcctgaac agacatacgt tcacggacat tacgttccat acgagaaagt
     6121 cccaaacgta cttggttggc aagcaattca ccaaccgcac ggatacgacg atttccaagg
     6181 tggtcgatat catctacacg gccaagtcct tcagccaagt tgaggaagta gctcatctca
     6241 gcaaggatat ctgcaggagt caccgtacga accttgtcat ctgggttagc attaccaatg
     6301 atcgttacga cgcgatctgg atcagttgga gcaacaacct tgaatttttg aagaacaaca
     6361 ggctcagtca caacggctgc atcgtttggg atgtagacaa tcttgttcaa gtcgccatcc
     6421 aaatggcttt caatgctttc aatcacgcta cgagtcataa tcgtaccagc ttctaccaag
     6481 atttctccag tttcagggtc taccaatggc tctgcaatgg tttggttgag caaacgtgtt
     6541 ttaacattga gttttttatt gattttgtaa cgaccaactg ctgccaagtc atagcgacgt
     6601 gggtcaaaga agcgagccac aagcaagcta cgtgagcttt cagctgtctt aggctcacct
     6661 ggacgaaggc gttcgtaaat ttctttcaag gcttcgtctg tacgagagtc cattggattc
     6721 ttgtggatat ctttttcaac agtgttgcga accaattcgc tgtcaccaaa gatatcaaag
     6781 atttcatcat cacctgagaa accaagagca cgaaccaagg ttgtaaatgg aatcttacga
     6841 gtacggtcga tacgagtgta ggtgatatct tttgagtcgc tttcaagttc caaccaagct
     6901 ccacggttag ggataacagt tgaaccatag cccaccttac catttttgtc tactttgtcg
     6961 ttaaagtaaa cacctggtga gcggaccaac tgagaaacga taatacgttc accaccattg
     7021 atgatgaaag tacccatttc tgtcatgatt gggaaatcac caaagaaaac ttcttgggtc
     7081 ttgatttcgc ctgtttcttt attgatcaaa cggaaggtta caaaaattgg tgctgagtag
     7141 ctagcatcgt ggatacgagc ttcttctagc gtgtattttg gttccttgat ttcatatcca
     7201 acaaattcca actccattgt gtctgtgaag tttgaaattg gcaatacatc ttcaaacact
     7261 tccttaagac cgtggtctag gaaagctttg aatgagtcag tttgaatttc aatcaaattt
     7321 ggtaagtcaa gaacttcttt gattcttgaa aaactacgac gggtacgatg tttcccgtat
     7381 tgaacgtcat gtcctgccaa gatgattctc ctttgtaaat aagttccaag ccttgtcaat
     7441 caggcttttc taatcgtcat atggttgtaa accccttatc accgtgtcct cttgacgaat
     7501 tttcagaatc tttaagcctc tgttacaaat gctcataatc ttgaaaaaaa gcacaaaaag
     7561 agcagctaaa tctgactttt tcagaagatt taactgctgt gagccttgtc tggacaatat
     7621 ttcagacaaa acctacgaca aatgattacc catattatac cctatttagc tagatttttc
     7681 aaggggtttc agtaggtttt tggtaaattt tttcccatag aaaacttggc atcacattcg
     7741 aatcacgcta tggtacaaaa aactgaaaaa actattgact gaaaatcatt ttcaaggtat
     7801 aataataaac gttaaggcgg tatagccaag tggtaaggca cggctctgca aaagcttgat
     7861 cgtcggttca aatccgtcta ccgccttcta taacttgatt tatcaggttt caaatgaaca
     7921 gaaagcccaa tttgaagggc ttttcttatt ttccctcgaa taaatacgta taactttaaa
     7981 aacttttgga gcgagtttgt ggcagagttc tttccatggc ataattccct tttgaaatca
     8041 attgaaaaaa gcttcgtcac gcctggtgac aaaagcctct agtttattct gtttcttctt
     8101 ctatttcgac ttctgtgatt tggactttta ccttggtaac acgtccattc ttcactttat
     8161 cattggttaa gataaactgt ttgttttggc tgaccaattc atagctgagt ttctcagtcg
     8221 ttggaatagt tcccactcct gtcaaataat aaccagcgat ggtatcaacg tcatcacttt
     8281 ccagttcaac atcaaagtaa ttattgaaat cattgagggt catagttcct tgaacaatgt
     8341 aggtatcctc gccaatctga tgaacatcta ttgctgcttt gtccgtttca tcatcaattt
     8401 caccaacaat ttcctctaag aggtcttcca aagtcaccaa accagccata ccaccgtatt
     8461 catccagcaa gattgccatt tgtctttggg tatttcgcag ttcttttagc aagtcatcca
     8521 caaaaatagt ttcaggtaca aaaagtggat cttgtaaaat tctcttccaa acaatattgt
     8581 caaaaccgtc cacaaagcct gccttaagga gactcttggt gtgaatgatt ccaattacat
     8641 tgtccttatc cccatcataa accgggatac gagaataatt ttgttttaaa atactttgga
     8701 taatggcttg actatcatcc tgaatatcca ccataaaggc atccgttcga ggcaccataa
     8761 cctctcgtgc catcagttca tcgagcgaaa agacaccttg tagcatctca atctcatcag
     8821 catccaatgt ttcttcacta tttgtcagca tataggcaat ttcatcacgg gtcatctttt
     8881 catccgcatc atcgaatgac ataggagtca aatggctcaa gaaattggtc gaagcagcta
     8941 aaagccaaac aaaaggactg actagttttc cgatcccaat gataatcggc gctgtacgaa
     9001 ttgccaaggc atcctttaga ttaagagcga ttctcttagg atataattcc ccaaaaacga
     9061 tggaaatata ggtcaaaaat gccaaggata gaaaagttgc cacggcttgt gctgtttcgc
     9121 cattcccaag ccaagaggca atcacacgtc ctagagtatt agttaaactc gcccctgata
     9181 agattgtaat cagggtgatt cctacctgaa tggttgataa aaagtggtta ggattttcta
     9241 gtaccttcag caggcggatg tagcgtctgt ctccttcttc cgccttttgt tcaactcggg
     9301 cacgattaag agaaaccatg gccatttctg tggctgagaa aaaggcattt aataccgtca
     9361 agataaacaa taaaacaaat tgtagcaaca aattctgact acttgggtct tccatagttc
     9421 ttctcctaaa atagtatctt atcctttatt atatcacaaa atttaatcca atacatatta
     9481 ttttttctta ccgtaacttc tcaaagtaac ttccatctct ctcccaaaac tggaagttag
     9541 ttttagaagt tgctattttt tcttagtacc tgttctagcc ctgcttaaac tagtaaaaag
     9601 aactcctaca tccataagtg acataggagt ttaggcttat cttttactga gttacagtta
     9661 cttctccagt tcggtaatcc agttgaagtc ccttttgaac attgggaact agaacattga
     9721 attccaattc tccatccgaa gacttggctt caatctgtga agctgaggga actaaatcct
     9781 cgtttgaagc gtagtaaagg gttacacggt aacggacacg cttgttttgg tccaaggctt
     9841 tacgcacctt gctttcatag tagttttgac cagtcgaata ctcggcttgt gcctgatttg
     9901 cccaggctgt ctgaacagca atgtttttag gattgcttgt tgaggcatca aaaccatcca
     9961 aaccaccgat taaggcatag cctaacaaat gacctctatc gactgcatgg gtataagagc
    10021 cctttagatt cttgacctga tgccaacctg gaggagtcca agaagttgaa ccattcccag
    10081 tttctttacg attcttgtac tgacgagtgg ccttagacaa gagggcatta gctacggttg
    10141 gaacagtttc cttgcccact gtctttgttt tattgtcagc gtagggctta cttgaaacct
    10201 tggcatctag atttgtttta ttaccattga cgataaaagc acctgagcca ttccactcca
    10261 gactcccctt tatttgactc ttgactgcgt ctgttaagac actctctgcc aatgcttgac
    10321 taggagcttc agacgcttgt tttttctgac taagattggt tttgggacta ttaggtgccg
    10381 acggcatctg cttgatataa tagctccctg tagacaaaag caataacact agcagtccga
    10441 ttagtgtctg tcttgttttt ttgttcatat ttctccttat ctttagaaaa aggctggttc
    10501 tccagcctaa tttcctgtaa atttatgaat caattcctgc cattttgctg gagacaggat
    10561 agcccatgga tcttgaccct tgcccaagat tccataacct accattaaac cgattcctag
    10621 agccagagta cctaaaatca gtactatgat gactaaaagt aaacgcttga ctacgtagct
    10681 tgatttctta ttcatcttca tcacttgcct caatccgctg aatcttagca cctagctgcg
    10741 ccaacttctc atggaaaccg tagtaacctc tatccaagtg aaccaattta ccgaccacag
    10801 tttctccctg tgctaccaaa cctgtcaaaa tcaaggctgc actggcacga aggtcagttg
    10861 aaagaacttc tgctccctgc aaaggctgtc caccaacaat acgagctgta tcacggataa
    10921 tctcagaatg caagcccatg cggcgcatct cttctaggtg ttggaaacga ttttcgaaaa
    10981 ctgtctccac catggttgat tcgccttttg caactgtcat caaggctgta aattgagcct
    11041 gcatatctgt tggaaatcct gggtggggca aggttttcac atgaacagct tttagatttt
    11101 ctagttgaga acgaacacga attccttcgt cttcttcaat tacttcaaca cccatttcaa
    11161 gtaacttggc aatcaaggga cggttgtgct cccagacagc gtctcgaatc aagacatcac
    11221 caccagtcat ggcagcagct accataaagg ttcctgcttc gatacggtct tggactacat
    11281 tgtgagtcgt accatgaagt ttctcaacac cagtaatggt tatagtctct gtaccagcac
    11341 ctttgacctt ggctcccatt tcattaagga gaatggctaa gtcaacaatc tcaggctcac
    11401 gcgcagcatt ctcaatcact gtcaccccat cagccagagt cgctgccatc atcaagttct
    11461 gcgttgcacc aacacttgga aagtccatat agatatgagc gccatgcaag cgttctgcct
    11521 tggcttcgat gtaaccagct gtctgactaa tcttaacccc catagcttcc agacctttca
    11581 aatgaagatc aataggacgg ctaccaatcg tacaaccacc tggcatggat accttggcat
    11641 gacccacacg ggcaaggatt ggccctaata caacgatgga ggcgcgcatc ttgctgacat
    11701 acttgtaagg ggcttcctca gtgatgtcgc cagtagcatc caccttgaca agatgagctt
    11761 cctcatcaaa gtcaaccttg gcattcaaac caccaactac ctgattcata ataaagacat
    11821 ccgacaaaat cggaacattc tgcaagacgg tctttccttc acttgctaga atagtcgctg
    11881 ccaacaaggg taagactgca ttttttgctc cctcgatcgt cacgcttcct accagacgat
    11941 tatcgccacc ttgaaccaca attttatcca tactcgtttc ctttactctt gatttcataa
    12001 taatcctcta agcgcttctt gccacaaatg atacaggcta ataaagaaag aactcaggat
    12061 gtagcctatg acaatgctaa aaaaggctac taataaacga acttttcttg tattatcatc
    12121 tgtcactttt aaaaccttct cccatctaac aagatccttt aaaaggtaaa aactcacata
    12181 aataaagaac atatgactac ttagagtgaa taataattga accatttgac tattatacca
    12241 tcttctctgt ttgtgcgcta gtttggaaac ttcactcaaa aattagggat tgtttttcaa
    12301 gtaatcaatc gcatcttgta gggttttaac gggcacgatt ttcatatccg tcttgattgt
    12361 tttagccgct tctagggctg tttggtagtt gtttttcgcg tccggatgcg ccttttgttc
    12421 ttcttcgcta acagggttat caggagcaaa gaaaatagca gcacctgccc tagccgaagc
    12481 tacaaccttc ttatcaatgc ctccaatgtc tcccacatta ccatcgcggt caatggtacc
    12541 tgtaccggca acaatacgac cattacgaag atctgggtga gctatttgag tatagatagc
    12601 tagactaaac atgagaccag cacttgggcc gccaatacca gctgttgaaa agctaattgg
    12661 gacattgctg attacctctg tacgatcaat caagccgatt ccaattccat ttttgccatt
    12721 ttccaaggtg ataatctttc cttctgcaga cttggtttgc ccatcctctt cataggtgac
    12781 cttgacggaa tcccctaatt tttgagaact gacgtaatca atcaagtctt tggaactatc
    12841 aaaggtctga tcattgactg ctgtgactgt atcagagata ttgagaatcc ctttaaaggt
    12901 tgaattatcc gtcacattca aaacataaac tccaaagtac ttgagttcga tatccttacc
    12961 agctgttttt agtccttgat acttggccat attttgcgat gtttgcatgt agaattgatt
    13021 gattcgcata aattcaacat cggaagaacc acctgtagtc tcctgagcac tacgaatatc
    13081 tgtaaaaggt gtcaaccaag cataaatcat atgagctaaa gtggcatgtt gaacaccaac
    13141 cgtaacgaat tgataggcac cagcttcctt atcttctgtg tcatttactt taaggacttg
    13201 gcgaatatct tccgaaccac ctggaacctc tatataataa ggcaagggca ctacaaatgc
    13261 caagaaagtc acaatcaagg ccgcaatgac gtataagggc catctaatct tttttttcat
    13321 ttcttatttc ctccaaaata ctctcgggaa catggcaggc aatatcctga ccaaacttca
    13381 aaagctctcg aacacctgat gaactgatat agagatgttc aggccggcta tgtaaataaa
    13441 tagtctctat atcagaagac agctgatgat tgtagtaatc aaaactagct tcatattgca
    13501 aatccgccgc attcctcaac ccccgtacta ggaaagtagc ccccagtctt tttgcaacat
    13561 cgaccaccaa ttcatcatga gaagccacga cttcaacatt ttccaaatgt cccaaagcct
    13621 tttctagccc ccgtttacga ttttcaagag ggagaaatcc ttgtttgtgg ggattaaaaa
    13681 aaatacccac ataaagctta tcaaaaagtc tgctcgcccg ttcaatgata tccagatgcc
    13741 catttgtcat cggatcaaat gagcctgtga ataagccaat cttatctgac atagactgtc
    13801 accttactaa ttccataaat cttttccttc cagataccca gacaggcaat ctcttctgga
    13861 agttcaacgg ccttatccgt ctcgcacaca accataacat cttcagaaaa aagctctctc
    13921 ttagccattt tttcaatatc tgctacgatt tgttccttgg catagggagg gtctaagaaa
    13981 acgaggtcaa attccccaga tacctgttcc aatgccctct ctgcatccat cttgaggagt
    14041 tgaaattttc caacttcctt ggtcatctgg atattttcag ccacgatggt ctgagcctta
    14101 cggtctcgct ccaccaaaac agcactggac atgccacgcg atactgcttc gatagataaa
    14161 ccaccactac ctgcataaag gtccaagact cgtccccctt caaagtaggg accaatcatg
    14221 ttaaaaatgg ctcccctaac cttatccgaa gtaggtcttg ttgtcttgcc ttctagtgtc
    14281 ttgaggggac gtcccccata gattcctgat acgattttca tactgtttat tataccaaat
    14341 tatagacaaa aagagaaaga aaaccgaacc ttgcggttcg attctctaca aaatattttc
    14401 gtaagtatcg cgtacttctt gaggccaaac acttgtttgc acttctccga tgtgtctctt
    14461 gcgaagtagg aacatggcca tacgagattg tccaattcct ccaccgattg tcaatgggaa
    14521 taggccattc aacaaagact tgtgccattc caattccaag cggtcttcat cacctgtaat
    14581 ttccacctga cgtctaagag tttcttcatc tacacgaatt cccatagaag acaactcaaa
    14641 ggctccacct aaagactcat tccagacaag aatatcacca tttagaccct tgtagccatt
    14701 ctcagactcg cttgtccagt catcatagtc tggtgcacgt ccatcgtgcg gtttaccatc
    14761 tggcaactcg ccaccgatac caatcaaaaa gacggctcca aattctttac aaatcgcatt
    14821 ttcacgttct ttcgatgtca agtctggata gcgttctacc aattcttctg tatggataaa
    14881 ggtgatttgt tttggcaaga tagactcgat gtcatagcga gcttcaacag ctagctcagt
    14941 caggcgaata gccttataaa tcttctcaac tgtttctttt agataaacga tgttacgctt
    15001 accatttggg ataaccttct cccagtccca ctggtcaaca taaacagagt gggttgcatc
    15061 caaggaatcc tcatctggac gaagggcttt catgtggaca aagagaccct ctccttcacc
    15121 aaagccaaaa cgagccaagg tgtggcgttt ccatttagca agtgagtgca ccacttcata
    15181 agtagcatca gggatttgga gaaccttgac cgatactgga ttttccacac cagacaggtt
    15241 gtcctgcatt ccgtcaccga ccttactcaa gataggacct tgaacttcga caacttctag
    15301 cttatctttc aaatactggg taaaagtgtt tttgacaaag gaaatttctt cttgttgatg
    15361 gataaaactt ttcttcatag acaactcctc aaaaatttaa taaacgtatt atacacctat
    15421 ttccaagaaa agaaaagaga aaatttaaaa aaaatcagtg tcactcaaaa cactgatttc
    15481 atagaccttt tagtcattgc gaccaaagat acgtagaata cttaggaaaa gattgataaa
    15541 atcaagatag atgctgagag ccattgacac cacccagcct gtcgctacac gaccttgtga
    15601 ttgttcatag gcgaggcgaa tcttttggtt atcccaagca atcaaccctg aaaaaaccaa
    15661 aaccatggct acactaatca tataatcaaa gaagccacta gccaagaaaa tattgactac
    15721 catagcaatc agtaaaccaa tgagagctgc catcattgct cgaccaatcc cactcaagtc
    15781 tttcttggta aagataccaa ctgccgccat gacaaagaag agaagggcgc tcgatacaaa
    15841 ggcagataaa actgtacccg gagtatagaa agccaccaca aaactaaggg taaagccgtt
    15901 taaaacggag taaagtaaaa atactggaag agcagctgga ctatttcttg aagccatgct
    15961 actggcaacg aaaaccaaag ccaactccgc aaaagtagca atggtcaacc agagacgccc
    16021 ctgcatcaac aagtaaacca actgagactg aaagaccgtc aacataaggc ctgataccaa
    16081 agcagatagt ccgattccca gaccaacaaa ggcataaacc ttagcgtaaa attgattgag
    16141 acctgcacgg tcatgtataa tagtgtgatt catccttttc tccttttctg tgaacatctt
    16201 tccttgatta taacaaaaaa agttaaaatt agcttaaatg gaagattaaa ataccggctg
    16261 caactgcaac attcaaactc tcggcctgcc ccttcatgct aatatggacc aactggtctg
    16321 cactttcagc cataagggga ctaattcctt gaccctcatt tcccataact agtacaaaat
    16381 tttctataga aggcagttct ctgtaatcaa cagaatcttt agatagggtt gttgctagca
    16441 ctgggatagc taccttttta gtttcgtcaa gaagcgctgg actagtcatc cggtaaatgg
    16501 gcagatggaa atgactacct tgcatggaac gtagggtttt gagactgtag atgtctgccg
    16561 acttatctga aacaatcact cctgtaaaac ctgctgcatc cgcagttcga atgatagttc
    16621 ctacattacc gggatcttgc acatcttcca aaaacaagaa cttgccctga ctcaaatcag
    16681 cttgtcctac ttcttctttt tgaaccaccg ccacaattcc ctgtggagtc tgagaatccg
    16741 ccaaatctag caaaatatcc tctgaaatcc agacagtttg cggaaaagca gctaactgat
    16801 ctcgataatt ttctagggca aagattttct caatcgtcac tccagcttgg acagcttctt
    16861 caaacaagtg ccagccttca atcaaatagg cagacttgcg gtattttttt tggtgtaatt
    16921 tcttggcatt ttttaccaca gaattggctt ttgaggttat aatagtcata gaaatattat
    16981 aacacaatca aaggggtttg gtatgcaaaa ggttagaatg attgcccaag gtagggtaca
    17041 gggagtcggc tttcgttggg gtgtttacag cttggcactt gaaattggtg gcatcacagg
    17101 tcgagtatgg aataacgacg atggcacagt ggaaatctta gcccaagcag actcatctgc
    17161 tatcatggca aaatttatcc aagaaatccg aaaaggaccg acaccttttt caaaagtaag
    17221 ctacttagat gtcaaactaa gcaactttcc tccctactct gactttaaaa tcgcaaatta
    17281 ggtctccaga actattgtat attttgtaaa aaaacagtag aatagaaagg tataattttt
    17341 aaagaaggaa taaaaacagt gaaatctatt aaacgttttg cactctcagc tatgggagcg
    17401 gctatgttgc ttgtcttgac tggttgtgtc aatgtcgata aaaccacagg tcagccaaca
    17461 ggatttattt ggaataccat cggagcccct atggctgaag ctatcaagta cttcgctact
    17521 gataaaggtc taggctttgg tgtcgctatc atcatcgtaa ccattatcgt gcgcttgatt
    17581 atcttgccac ttggtatcta ccaatcatgg aaggcaacgc ttcactctga aaagatgaac
    17641 gccctcaagc acgtccttga gccacaccaa acgcgtctca aagaagcgac tactcaagaa
    17701 gaaaaactcg aagcccaaca agctctcttt gctgctcaaa aagagcacgg tatcagcatg
    17761 tttggcggtg taggatgttt ccctatcctc cttcaaatgc ctttcttctc tgctatctac
    17821 tttgctgccc aacatactga aggggttgct caagcaagct acctaggcat tcctctaggt
    17881 tctccaagta tgattttggt tgcctgtgct ggtgtccttt actatcttca atcgctcctt
    17941 tcacttcacg gagtagaaga tgaaatgcaa agagaacaaa tcaagaaaat gatttacatg
    18001 agcccactca tgatcgtcgt cttctccctc ttctcaccag ctagtgtcac actttactgg
    18061 gttgtcggtg gtttcatgat gattctccag caatttatcg tcaactatat cgttcgtcca
    18121 aaacttcgca aaaaagtcca tgaagaactg gcaaagaacc catcaaaagc aagtgctttc
    18181 tcaacaccaa gtggacgaaa agacgttacc cctgaacaac caactgctat tacaagcaag
    18241 aaaaaacaca aaaatcgcaa cgctggaaaa caacgttcga gataagaaga aaaaggctta
    18301 gctgatttgc taagtctttt tgcaagtcaa aagcccgatg tttccatcag gcttcttttc
    18361 tatccatgta cacgtttcat ataatcttga taactttcgg tatccatgag ttttttagcg
    18421 ttcttgacgc gatctgctgt tggtggtttg actccttcga gggaatatgg aattccaagt
    18481 tcacgccact tgaactcacc catggtgtga taaggtagaa tttcaaactt atcaacattt
    18541 ttgagggtct tgacgaactt accaagttca atcaagtcat catctctgtc tgtcaatcct
    18601 ggaactagca cgtggcgaat ccagacaggt tttccaatat ctgatagata ctgggcacaa
    18661 gccaagatat ttttattggt ttggctagtg acaatcttgt gctgttcttc gttgatttcc
    18721 ttgatatcca aaagaaccaa gtcagtgaca gccatgagtt tgtcaaactt ctcaaggtaa
    18781 cgcggtttat tacggaaagg aagagcacag gtgtccaagg tacagtggat tccttgttcc
    18841 ttagccttgg tgaagagagc aatcaggaaa tcaatctgca agagagcttc tcctccactg
    18901 actgtaatcc cacccttatt tccccagaaa ccacggtagc gcaaggcctc tgtcaagaca
    18961 tcatctaccg tccgttcacg tgacttattg gactccatag cccaagtgtc tgggttgtgg
    19021 caatactggc aacgcatgtg acagccctgc aaaaagacaa taaagcgaat accaggccca
    19081 tctactgacc caaagctttc tgtcgaatgc accatccctg tcacttgtcc ataatcaatt
    19141 gtttcttcag acatatcgtt accttccttg aaaacgtttt atagttttat tatatcacga
    19201 cttgaaggaa aaacaagatt tttgcctatt ttttagaaag caatctgttt ttcaatttca
    19261 ttttcaatct attatcagat tattcttcaa aatcgaagcc atataaggtt gcaaaatagt
    19321 cctcagggcg ctctgcacgg cggatttgac gggctttacc ttcttcggta tagaggattt
    19381 ccgcagaacg taatttggca ttatactggt agcccattga aaatccgtgg gcacctgtat
    19441 catgaattac cagcaaatca ccgatttctg tatgaggcag ttcgcgattg actgcaaatt
    19501 tatcattgtt ttcacagagt gaaccaacca catctaccgc ttcagctggt ccatctggat
    19561 gggtcacgtt gctaatatga tggtaagctc cgtacatagc tggacgcatg aggttgactg
    19621 ctgaggcatc tacacctaga taggtacggt aggtttcctt cttatgagtg actcttgtga
    19681 ctagagcacc gtgaggtgcc agcataaaac gacccaattc ggtgaaaatc ttgacctgac
    19741 caagacctgc tgacgtaaga acttcttcat acaccttacg aactccctca ccaatcaagg
    19801 cgatatcgtt cggctcctgg tctggacgat aattaacacc aataccgcca gaaagattga
    19861 taaagtctaa cgaaatgccc aacttttcct tgatttcaac agccagttca aagagctgac
    19921 gagccaactc tggataatag agatgggtca cggtattgga cgctaggaag gagtgaagcc
    19981 caaaagtctt ggctcctttt tccttcaaga tagcaaaggc ttcaaagagc tggtccttgg
    20041 tcatgccaaa cttagcctcc ccaggattgt ccataatgtc tgtccccagt tcaaaaacgc
    20101 ctccaggatt ataacgacaa gagatgattt ctggaatgcc tgctactctc tccagatgtt
    20161 caatatcttc aaaggcatcc aagttaatgg tcgcacccaa ttcacgtgca taggcgtatt
    20221 ccttgtctgg cgtgttgttg gaagagaaca taatctcaga acccagaaag tccagtttat
    20281 ggctcatcaa aagctctaca taactagagc agtccacacc acaaccttct tcttggagaa
    20341 ttttcaaaat agctggagtt ggagtagcct taactgcaaa atattcctta aagcccttgt
    20401 tccacgaaaa agcttggttg acagctcttg ccttctcacg aatccccttc tcatcataca
    20461 agtgaaaggg agtcgggaac tcggcaacaa tcgcttctaa ctcttctcta ttgataaatg
    20521 gtgttttcat aagcttcctt ctattctgtt tgcaaagaaa ggctgggaca atcgttccaa
    20581 cctttagttc tatctcttat ttatcttcgt caaagatatt gccaacctca acatcgatgg
    20641 cttggttctt gacatcaata ttggttacct tcaagagtga cttgtagtca aactgctttt
    20701 cacgttcttc ttgggcattg tccgcaaaga ccgctacacc tgccagttct gagtcgaact
    20761 cgcgcaagag actaatcata ccgttgaccg ttccgccacc tttcaagaag tcatccacaa
    20821 tcaagacacg gctgcctgcc ttaagactac gttttgaaag gaacattttc tcgatacggt
    20881 caccacttga acctgaaaca tagttgacgc taacagttga accttcggta attttcaggt
    20941 cacggcgcac aatgacaaaa gagacattga ggacattggc aactgcattt gcaagtggca
    21001 cacccttagt tgctacggtc ataaccgcgt caattttttg gtccataaag cttttggcaa
    21061 taatacgacc aatatttttc aagatggctg gtgtgctaag caaatcagac agatagatat
    21121 aaccacctgg caagatacgg tcactttctg acaacttggt acgcaagtct tcaaccattt
    21181 ccttagcatc ctgactcgaa atagacggtg tgaagatgac acctccgcca gccccagtca
    21241 ctgtctggat atgaccgatt tcaatttcct caaaggcgcg tttgataatg acgatatctt
    21301 ctgagatgga tgatttagca gactcatact tttcagcaaa agtattgaga ctagttagtt
    21361 tataaggatt attaatcaaa tagttggaaa tgacaaccat ccgatcactt cttcttaatt
    21421 tcatttctat ttcctattcc atttttattc aaaaaatcaa aaagcaaact agaaagctgg
    21481 tcgctggtgg ttcaaaacac tgctttgaga ttgtcgatag aactgacaaa ccctgtaata
    21541 tacctgcata tatacatacg acaaggcgat actaccctag tttgaagaga ttttcgaaga
    21601 gtattcattt ttgtctttta cttattatac catattcaca taaaaaaacg aacattctta
    21661 tcctaaaaaa tgctcatttt tcttaaatta ttaatctaaa tctggtttat agaaggaacg
    21721 attatccata gcgaagattt tattggacat ctctccttta tccaccaaag ccagagctgt
    21781 tgacatcatc atcatgcttg catccagatt gtcaatcata tggataatct ctgcctccat
    21841 aatacgtgga cggactggac ttccatattc aagcaagccg tggtgactga ggatgacatg
    21901 acgaagcaaa acgacttctt ccttggtatc gtcgatgccg agttccataa ctgtcttggt
    21961 aatttcgcta tcaatgagag cgatatgtcc aagaagattg cctcgcactg tgtactctgt
    22021 ctggtctggc cccgtcaact cgatgacctt agctaagtca tgcaacataa tccccgcata
    22081 gagcaggctc ttattgagct gaggataaac ttcgctaata gcgtctgcca aacgcaccat
    22141 ggtcgccgta tgataggcca agcccgtttc aaaggcatgg tggttggtct tggcagctgg
    22201 ataggagtag aattccttat catacttggt gtagagattt cggacaatcc gttgccagac
    22261 aggattttca attttgaaaa tcatttgcga catgtagtca cgaatttcct tgacatcaac
    22321 tggtgacttg accttgaaat cagctgggtc attgggttca ccagcttgag gcaggcggag
    22381 agtaatttga ttgacttgag gggtattgtt ataaacttct cggcgtcctt tcatgtggac
    22441 aaccttacct gcggtaaagg cctcaatgtt atgaggttgg gcatcccaga gcttcccatc
    22501 aatctcgcca ctatcatctt ggaaggtaaa ggctaggtag tttttcccag ctcgagtttg
    22561 cctcaggtca gctgatttga ttaggtaaaa gccttcaaat aactcatctt ttttcatgtg
    22621 actaatcttc atattcttcc tcattttctt gaaaatggag tagatcaagc gcaggctcac
    22681 cttctgacaa ctcaatgtga cggagcgtcc gctcgatagc tatggtacga cggtttaata
    22741 attcatcaat attgccagag gcatgttgga gatgtttttg tgccttgacc agaataccac
    22801 caaacttgcc aaactcggtc ttgacactgg caagagtctt gctgatatgg tcggcactct
    22861 tttggatatt aagggtcttg aaaccaactg atagggagtt aagaagggct gatagggtac
    22921 ttggtcctgc aacaataatc tgttcttccc gtctcaaatc atcaaagaag accggattgc
    22981 ggacgatttc tgagtagaga ccttctgtcg gaacaaacaa gattccaaaa ttggttgtcc
    23041 gaggcggtgc tatgtacttg ctcttgatat ccttggcaaa gcgcttgacg cttgctagga
    23101 gtgacttacg acagcgttca atctcatcct tgtcacctgt ctcataggct tcttccaagc
    23161 ggtaataatc tgccagtgga aacttagagt caattggaag atagacgtat tcttggtcgc
    23221 cttgtccggg taacttgatg gcatactcca ctcgttcact agagttttca accgttgcgt
    23281 attctcgttc gtactgggca ggtgtcatga tgtcttcaat aatttgcccc agttgcaatt
    23341 ctcccagaat ccctcgcgtc ttggttccag agagaacctt gttaagagct ccgacatcac
    23401 gggcaactgt ctgcatttct ccaagaccac gattgacaga ctccagttgt ttagaaactg
    23461 tctcaaagga agcctgtaag cgtgtctgca aggtcttttc tagtttttcc tcgaccgtct
    23521 ggcgcatttg ttccaaacgt tgctcatttg attcctgcaa ggcttggaga cgttggtctg
    23581 tcttgtctct agtttggagg agattatctg tcatttcttg acggacttgg gtcaggcctt
    23641 ggtgcaattc aatccgcact tcttgcaaac ggtcgctgac agccacttcc aaatcttttt
    23701 ggtctaactg gctggcttgt ctggcttggt caaagcggta atccaactgg tctgacaaat
    23761 gatctgcctg atcctccaag ctcttactta agtgtttctc ctgcctatcc tgcctttgcc
    23821 aaatcagaaa gagaccagct aggttggcaa ttaataatag aataagtaaa ctttccatcc
    23881 tacctcctat ccttgctatg cagtacgacc acataaccat ctgggcaagt taccgacact
    23941 tccctatcta tatattcgtt agaagcgtac acttttttaa agaaaaaatt ttcctctgtc
    24001 aactcatact tggctccaag aatagtcagc tggctatccc gaactggcat aaaggctaga
    24061 tagtcgtagt ctgaacgagg ttctagctga ctggtccctt ctggacaata agtaatcaag
    24121 ttttgcccat cctcaatttc tatttgatgc atatagggtg ccaacttagg attgctaggc
    24181 agaaagacat tggccaacat atggtcaata cggccaccca aggcaccgaa aatagtaacc
    24241 tgagcctgag gattttgttc aaagatggtt aagagagcca attccaaatc ggtatcatct
    24301 ttttctggtc gtgcttggac aaaatgctgg gcacgttttt gaatcacctg tcgctcttct
    24361 tcggtcacag aatcaaaatc cccaactgct agagcaagag gcaagtcttc ttccaagacc
    24421 cagagcgagc ctcgatccac cccaacaaaa gcatcaaaat ctgtccgata atgaccgcgg
    24481 tctccgcctg caaaaacggc aaccctagtc cagttgtttt ctgagagttt gtactcgctc
    24541 attgacatct cccttaaaga cataggaacc tgctacaaaa acggtcgcac cagcttcttt
    24601 agcttgggca atagtttggt catcaatccc accatccact tcgatttcaa agttcaaacc
    24661 tttttcctca cgaagagcaa ccaactcacg aaccttatcc atggtttctg gcagaaaggc
    24721 ttgcccacca aaacctggat taaccgtcat gactaaaact tggtcaacta gatgaaggac
    24781 gtgcttgatg gcttcaactg atgtgccagg attgataacg actgaaggct taactccgag
    24841 tgaacgaatt ttttggaggg cgccatgaat atgaggcgtt gcttctacat ggatactgat
    24901 gatgtctgca cctgcgcgcg caaagtcttc cagatggtgt tcaggattcg ccaccatcaa
    24961 gtgacagtca aagaccatct tactatgtgg acgaagtgct tcaaccacac cggcaccaaa
    25021 actgatttgt ggcacaaaat gactgtccat gatatcgata tgggcatatt ctgccccagt
    25081 tgcttctaga cgtttgattt cacgttcaaa gttggcataa tctgctgcca gaattgacgg
    25141 agcaatcttg tattgagaca taggtttctc cttattttgg aatttttttg ctgacttttt
    25201 tatgggtttc tctacgattt tcaatttcac taaggaattg caggtaattg tcaaaacgga
    25261 aggttgcaat aacaccctct tcaacagctg gtttgacggc acaagacggc tcatgggtat
    25321 gggtacaagt acgaaactta caatctcggc tgacagtagc aatctctggg aaagcctgat
    25381 tgaggtcttc agcccttgat acttcatagt ccagagatga aaagcctggt gtgtctgcga
    25441 ttttaccccc attgaggttg aaaaaactaa cagctcgagt agtatggcgc ccacgaccca
    25501 gactgtctga gatttctcct gtttcaagat tgaggtctgg tgcgattttg ttgagaagag
    25561 ttgacttccc aacacctgtc tgccccataa agaccgtaac cttgcctgtt aacaaagaca
    25621 ggagttcctc tttactggtc acaaagtcat agccgatgtc accataggtc tgctggtaaa
    25681 aatccagttc tcccctatct tccaacaaat ccattttgga aatatagaca atgggatgga
    25741 tgcccttgtg ctccaaaaga accaagaaac gatccagcaa attgctgtta aaatcaggtt
    25801 ccttgacgga catgattact acagcttgat cgatattgac aataggcgga cgaaccagac
    25861 tgtttttccg ttcgtgaatt tttaggatat agccttctga attttcctcg gcagagaaat
    25921 ctacccagtc cccaacataa ggggtatggc cttttttacg gaaattccca cgcgcgcgtg
    25981 tttgataaac ctggccatca ctctccacat agtagaaacc tgccaaggct ttaatgattt
    26041 gtccctgcat cttagagtcc ttgcccttta agtgcgtctg ctagacaggc aaattctgcc
    26101 aagctgagag cttccccacg cacacttggt gacaagcctg cctggtccaa agccttggtc
    26161 agcttgtcct tgacctcttc agtcttacca aagtaacctg tcaagttatt ccacaaggtc
    26221 ttacggcgat gggtaaaact agccttggaa accttaaaga agaagttctc atcttccact
    26281 gctacagctg gctctggacg gcgcaccatt ttcaagatgg ctgaatccac atttggcgct
    26341 ggcacaaaga ccgtacgagg cacgataaag gcaaccttgg ctgtcatgta atactgcacg
    26401 gcgatagaca agctaccgta agccttggtg ttaggctggg ctgaaatgcg gtccgctact
    26461 tctttctgca tcatgaccac aaactcacta aaaggaatcc cactctcaat caagtgcatg
    26521 agaataggcg tcgtgatgta gtaaggcaaa ttagccacta ccttgattgg caggttagga
    26581 tttttaaaat tttggatatg ttgcgccaaa tcaaccttga gaatatcttc gttaactaca
    26641 gtcacattat caaaatcacg cagggtatct gccaaaattg gcaccaaacg gtggtcaatc
    26701 tcaaaagcca tgacttgggc tgcacgctca gccagaaact ccgtcaaggc accaatacct
    26761 ggcccgattt cgatgacatt gacctggtca tcaatttcag ccgtatccac aattttttga
    26821 aggatattgg tatccgtcag gaaattttgc ccgaaggact ttttaaaggt aaaaccgtga
    26881 cgctccagca ctgccttggt cacgctataa tctgcaattc tcatctattc tcttttctat
    26941 tatctgttac tactattgta acacattccc cttgtatttt gggaatgaaa atcataccaa
    27001 aaaactatct ttcctcatga agaaagatag tcttggaaat ctttaaaagt ggccttaaca
    27061 gttctctcga aacatgcgtt caaaaaaatc agcttcttga taacttcgat tttcgggtag
    27121 ggtcaaatcc acatcctcct ccacatcatc cactgtcagt tcaaccgtct gaggtaaatt
    27181 tttcaaacga taatgctttt gcctttgccc ttcaatacta gcaaaatcaa gcaaatagcc
    27241 atgtactcgg atttgaacca aaaggagatg attggtcata ttttccatga tgaggatatc
    27301 ttctaaacca cctctggcac taggataatc agatactctg cgaatacgga ttttttcaca
    27361 catgagcaag tttcctcata tcaatgattt catcagcctt attccagact aggggattat
    27421 gggtcgcaat gatgatcatt cgattttcat ctttcaaatc caccaagaga ttcataacct
    27481 cctctgaatt ttcaggatca agagctgctg ttggttcatc tgccaaaatc aagggtggat
    27541 ttttcaaaat agtcttagca agggcaactc gttgggcctc tcccccagat aaagtataaa
    27601 ttttttgttc taaatccaaa taccctagat taactttttc taaagccccc acttgccttt
    27661 ccaaacgttc tacttttgag attttctgac caacaaaacc caaatccaaa ttttctttga
    27721 ttgattggtt ttctaagagg ccaaaatttt gaaagagata gcccatctga tctcgaaaat
    27781 actcacgagt gggaatggtt tttaaatctt tcccctgata gagaaccctt cctccatcta
    27841 tcttttctag ctttcccaag atattcagca gcgtcgtctt tccgcttcca ctctttccga
    27901 ttaaggcata aaccttgccc ttctccagct tgagattcaa accagagaaa atcgcgcggt
    27961 cattaaattt cttttccaat ccttgaatat caatcatctt attttccttt caatactgcc
    28021 atgctaccag cttcttcttt tttgtcctgc cttactaaaa tcaagagggc gttaagcgta
    28081 aacaaagcta caactagagc gctaatcaaa ccatctcttg tcaaaatact agataggact
    28141 aggccaagca agagaactcc tccttgcgcc agtaaatact tgccatgaag ctcatagatt
    28201 gtcataccag caagacgttt aatcataagc tcccgtctga actgctcaaa atagagaaga
    28261 ttcatggaat caaataacaa gatagccgta gacagggtca aaatcgtccc aataaggaga
    28321 gagtaaaact ccacccgttt gtcatttagt accttggcaa aaaatagctg gctttttacc
    28381 aagtaagaaa ccttgtgata cagaccttgc tcttttagag ctgtgatgct gtctccatat
    28441 cgatctagtt tcaaggcact attagccgta gttccccaca acatccctgc aacaggtctt
    28501 gttccagtcg cttgcggcgt tagaactaca ataatcggat cttttaggta ctggcgtgtt
    28561 gtcttgtacc catcattata gaggaaacgt tcctgtcctg tttctgtaaa agctagcctt
    28621 acctgtggga agtagtgttt ctgactcgtc acttctacac tttcagatga aaagttttgc
    28681 aggtaatctg taaacaatcc ttggtagtag gcagactgct ctcgcaagct ctcaggcaag
    28741 atcagcccaa actctccctc agacaagttt tgcatcttgt ccataaactc tgaagaaacg
    28801 gtaatctttt cttctatcag atagcgtggt gagacataga taacattccc tgacggtgtg
    28861 tagtcactga gacgattacc atctaggtcc acttctgctc catctgagaa attgtcaaca
    28921 ttgctcataa tataaaaaga gtctgtattg gctaaccgtt cttcagtaaa tgtctgccac
    28981 tcacgattat ccttacgcgt tccttcttca tcggcaaatg cactagacca accaaaggat
    29041 agacggtaac ggtctgagta ctggctccat ttattgctag ctctctccat ttcctgcatt
    29101 tcacggtagt ggggtaggag agctgtcgca ctcgatccga ccaccaatac agctaagagt
    29161 tgccccacca tcatcaatgt catcatacgt ttgagaggga gtttcccttt caatagatcc
    29221 accagactat tttcctgtaa accaagtaga tagacgacac ttagtaaggt agaaatccct
    29281 gccaagatca atccataaag tagaagagca atgatgacca gttgcaccgt tgccataaac
    29341 aaggcacctt gataccagag aatccccaat cccaaaatac tggataccag cactgagcag
    29401 ataagctgtc tcacatcttc taacactggt ctgagagcaa ctccaaacaa gctctcacca
    29461 gctattaagc gaatccctgc ctgacgaagg gatttgatcc gataaatcag ggtcagactc
    29521 ataaaggtca gcagaaaaat agctaaactc agtagcacca taggggtggc cgtcaatagt
    29581 aagactatcg aaaatggatc ttctccattc gaaacaaagc cttgatagcc aagttctttc
    29641 aaggtggtct gtaagctcac tccatccaaa cttcctgaca caatcaagta agaccctaat
    29701 aaatcactcg tttctgcact ctccttggag gaaatggtca gaccttctgg aagctctccc
    29761 tcaccataag tggcataggt aaagagtgtt tctccagctt catttggctc aacgattcga
    29821 cgggcgatga gactattatg ctctaccgcc aagtgactca aagcctctgt caccttttcg
    29881 cgactagcta ctggttgatt tatcatcatt cctgtcacat caacagaagg atagttacca
    29941 aataaaatct cttctcgatg gtcaacggaa gttatcacca tataaagcga taccaatacc
    30001 attgaaatca aaataaataa acgtttcatc atttcctcca aaaagttact tggtcactta
    30061 gtgagaaggt gaccacagtc actgcgatgt taagaagctt attaagttct ttctgctagc
    30121 aacatcttca aatatattat atccgttttt tcaagaacta tcaaggacat caatgtcctt
    30181 aaaatctaac tatttcatat tatttttagc ttatatctaa aaaaacagtt ctaaagacga
    30241 actttagaac tgttttgata cgaggacaag gacatttttg tcactgattc actatgttta
    30301 taaatatttt ttgacatcct cttgccattc ctctgtcaat ttctttacta gaaatccatc
    30361 tccaattagc ttcgcaaatg ttttagaaga ctgataaaat gattcagcta aactaaaatc
    30421 tctttctact cttaatgctt gcttccacaa aaacataaat acaagaggtt tcttttgata
    30481 atcttgagtt ttatccataa tttcttgtaa acacgataga taatcattaa aacaatcagt
    30541 tactcctacc ttttcaagac aacacattcc tgaaataaga gtatctctaa ttataaagta
    30601 attctctatt tgagttacct tcttctgttc caaaagaaag agcattagct tttcgatttg
    30661 atttataaat ttggcaagat cttttctaat gagcatttga taaaaaaaca aacgaatcaa
    30721 caacatatca tttgttgaat aaacttcttt ttctatgatc tgagacatat gttcctcaag
    30781 tattaactct gcatattcag gccttctagt tctagaagta tccaaacacg cctgcaatac
    30841 ttcacatgct aattgttctt ctttaggaag attatcaaag tatgttttat aaatctcttc
    30901 caaacacgct tccttcttat cgtactcctc ttctttacca tagattggtt cacgtaaaat
    30961 ctggtatttc aattctaaat aagcagaagg aagagctgaa aaatccggca taaggctata
    31021 aactggaact cctagccggc gagcaatata gtctaacttg ggcaaactcg gttgcgaagc
    31081 tccactttca attcgcgaca gttgacgaac tgtcagttct tgctcatctc cacaaaaatc
    31141 tggacgacta attccttttt ctatccgaat ctctttaatt ttttgaccaa ttaacatcag
    31201 acgcctcctc ttctacctgc ttcgaaaaat actgtgaaac ttaaaaaatt gtaacggtca
    31261 catctttcct actcataaga tttcatagct tcttccactt ctgccaaagt aaccccaaac
    31321 aactctaggc gtttgaggag ttgcttgccg ttagaatagc cgattcggag agtctctccg
    31381 agatattctc tacgcttaca gctgtctgcc cctgctagaa aaccaaggcg aatcaagtcg
    31441 ctacgactaa tgtcaaactg actctcatgt tcaaattgtt ctgtcacctg agctagagcc
    31501 gttttcaggt cttcatagct ggcatgctca attcccagag aacgcccctt ggtcttggac
    31561 ttgggaacag cttcatctcg cttgagaaag gcatgctgaa ctgttggaat gaccatcatg
    31621 atcatgcgcc gaatccgttc cccattaaaa tctgggtctg taaagacaat gactccatga
    31681 cgttggtgca ggcgctgaat ccgctctata tcctgagcat tgatggcaga acctcgagtc
    31741 tcataggtct ccacatcgaa ataacgtttg agattgaccg tatcatcacg cccttcaacc
    31801 acgataactt gagaaatttt ctctttcatt acttgctgtc cagtccaaaa attcgttctg
    31861 catttgcagt cgttgctacc gccagctctt ctgtcgtcat accacgcaag tcagcgataa
    31921 agtcgaccac atagcgagta taggctgttt tattttcacg accacgcttg ggtacaggtg
    31981 ctaagtaagg cgcatctgtt tccaccaaca tcttgtccaa aggtaactct ttagctgctt
    32041 cttggaggtc agttgccttc ttaaaagtca ccactcctga gaaggaaatg gtcataccaa
    32101 gatccacaaa cttctctgcc cactcaagcg tccctgaaaa tgaatgcatg ataccaccac
    32161 gaggaccaac gccctcactc ttgataatct cataggtatc ttccagcgca tcacgggtat
    32221 ggacaacaaa aggcaaatcc aagtccttag atagctgaat ctgacggcga aaaacctgct
    32281 cctgcacctc tttgggcgct gtcatccaat ggtaatctaa gccaatttca cctaaagcca
    32341 caaccttgga atgttttaac ttatccaaca agtaagcctc aacttcctct gtataagtac
    32401 cagcttctgt aggatgccaa ccaatagtcg catagagctg ctcatactca tctaccaact
    32461 ccaaggcatg ctcaatcgtc ggtttatcaa aaccaacaat attcatctgt gtcacaccca
    32521 tctcagcagc caaggcaatt tcttctgcct cacgacctgc aaattcttct acattcaagt
    32581 gtgtatgtgt atcaaaaatc atctcttcta acctcgtttt ctatcttcta ttataccaaa
    32641 aaagaggagg ggcacctaat ttttcggttt cccctcctct cttcaataga gagctattct
    32701 gctatctttt ctatccgata ttgcccatct cctattccac agttagagac agaagagatt
    32761 ggctacattg atagaatatt cctattcagc taaagtatgt gattatcttg ggttctcact
    32821 tttctcaccc atcactagga ttgatgactt ctcaaaatcc cccttataat ctaactttaa
    32881 gtagagaata gaattttcta gctgttctat tttctacgtc tagtctttct tcctagcaaa
    32941 gcaaaaccac ttgttgccac cactagacca agactagcaa gtgctgcact tgatgctgaa
    33001 cctgtgttag gtaattgttt gccagcaggt gactggtagg taacatcttg cattgttcct
    33061 gcagttgctt gcggtgcact tgttgtctta ggagcagcat taggagccac tggtctctta
    33121 ggaactgtaa ccatggcaat ggcttgcact ccaatcgttt ctgcttcctt cacatcttcc
    33181 atagttttcg cattttcaat ctctttgaga gctgcttgtt tttctgcttc cactcttgct
    33241 aaaagttctg ctttttcttt atcagaaagc ggtgcgcctt tgatttcatc ctgcttgtcc
    33301 ttggctgctt tttcaatggc atttttagct gattcttttt cagtagctaa ttgcttagaa
    33361 gcttgtgatt gagctgagtt ttgtgaacct gtgtgagctg atccacccgc ctggctacca
    33421 ccgccttcat tcgcattagc atctgagctt ccagtattgc ctcccttatc tccacctggt
    33481 gtattttcta gcttatactc tggtgtagct tcttgagtta cagattcacc tttcgcagat
    33541 tgttgaactg tatcttttcc tagaatcgtc actactgaac catctgggaa tgtgattgtt
    33601 gctgtaccat ctccagctac attgattgtc gctccgtcta atgctgaacc atttgcttgt
    33661 aaaatagcaa ccttaacttt ttcttcatct gttaaatgag ttatatcttt tacatctact
    33721 ctttgagcag gtgctttaac agttgatttt tcagcagtca gtttagtgct atcttgtgct
    33781 tttggatctt gtgctaccaa ttgatttcct ggaattgtaa tagctgaacc atctttaaag
    33841 acgattgttg ctgtaccatc tccacctact tcaactttat agcaaaataa aataataaca
    33901 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaaa cagaggtgta
    33961 ctattctagt ttcaatctac tgtaacatcg cttccgaatc cgtttgcatc tactgaactt
    34021 ttttacaaaa ataaaaatca gtagactcga gttcctactg atttttgata gaattcgttt
    34081 ggattatgca gccaaaactt tgcgtccttt acgacgacga gctgccaata cgcgacgacc
    34141 atttttagtt gacatacggt tacggaatcc gtgtttgcgc gcacgacgaa gtttacttgg
    34201 ttgataagta cgtttcacga tgaatacctc ctcatagatt ttgtattcgt ttagccggct
    34261 ataaagtgat cagttactaa acatacttta ctattctatc tgattctttc acttttgtca
    34321 atagctctag gcaaatgttt ccagcttttt cgaatgcaag ccctatctac gatactgttt
    34381 caagaaacga gtcatctttt cttggatttg ggctaactca tcaacacgag gaaggtaaac
    34441 gatacggaag tggtctggtt cctgccagtt aaagcctcga ccatgaacca agagaacctt
    34501 ttcctgcttc aagaaatcaa ggacaaactg ctcatcatca tcgatacggt acatattgcg
    34561 gtcgattttt gggaagatat agagccccgc cttgggttta acggcagaca aacctggaat
    34621 atcttgaatg gcattataga tgaaatttct ttgctcgtag attcgtccac caggaagaag
    34681 caattcatcg actgattggt gaccccccaa ggaagtttgt acgacttgtt gggccaaaac
    34741 gttagagcaa aggcgcatat tggacagcat attgagccct tcgatatagc ccttaacatg
    34801 agtcttaggg ccagacaaga ccatccaacc cacacggaaa cctgctatgc ggtgggattt
    34861 tgacagacca ttcatgctga cacagaagac atctggtgcc aagctcgcca caggcgtatg
    34921 cacatgtccg tccattacca tgcggtcata gatttcatcc gcaaagataa tcaaatcgtt
    34981 ttgacgggca atctcaataa tctccagcaa gagttcctta ggataaaggg ctccagttgg
    35041 gttattggga ttgataagga caattgcctt ggtattggaa gtgatttttg acttgatatc
    35101 gtcaatatct gggtaccatt ccacagcttc atcacagata tagtgaacgg catttcctcc
    35161 agctaggctg acagccgccg tccatagagg atagtctggc attggcacta agacctcatc
    35221 cccattgtcc aaaagcccct gcatggacat gacaatcaac tcactgacac catttccaag
    35281 gtaaatatca tcaatatcta cgtttgggaa tttcttcagc tggcaatact gcatgatggc
    35341 cttacgggct gagaaaatcc ctttggagtc agaatagcct tcactatcac gcgcattcat
    35401 aatcaagtca tgaatgacct cgtctggcgc tgtaaagcca aattctgctg gatttcctgt
    35461 attcagacgt aaaatctttt ctccattcgc tcgcatccgc atggcttctt ccaatacagg
    35521 accacggata tcataagcaa catgctctaa cttactagac ttgttatatt ctttcatctt
    35581 gtttcctcaa ttcatcagga tagtaacatt ataccactag aaagagaatg cgacaagttt
    35641 actgaaatgt gttactagtt tcgtatagaa gaaaaacctt gctaaaggcg aggttaagaa
    35701 cgtagagata gcaggtattt tgcaaagaca catcacatac caaaagataa acctagaaaa
    35761 aagcaagcct agaaaggagt cataaagaag attaaacatg aggaaaagcc cccatatcat
    35821 caagtaatcc caacgcccac ttcgttttgc aactagaaat aagattagat ggtagagtac
    35881 atgaaaaact aaaaatccaa taaaacttgg ataaagaagc ggtaccagac tctctaattg
    35941 acagatcatg atcacttcta ccaagactga aactaggata attccataat aaaggaaatt
    36001 tgatttttga aaagagtttt tcatcattct cccttccttc acttctttcc tgctatcctt
    36061 tcttttattt tatcatatat ttatatagag tttaagccct aatattcttt tgaaaaatac
    36121 tttttttgct ttacttctat agtgaaaagg tttacaatta tagtaagaaa atttcaggag
    36181 gtttcattat ggcacaacgt taccaaaata tcatggtcgc aatcgatggt tctaaggaag
    36241 cggacttggc ttttgtcaag ggagttcatt ctgctctacg aaacgacgct aaactcacca
    36301 tcgcacatgt cattgacaca cgcgctctcc aaagcgtatc cacctttgat gctgaagttt
    36361 acgaagaact ccaagtcgac gctgaaagtc tgatgaaaga gtacgaaaaa cgtgctaaag
    36421 atgctggggt agcagatgtt catatcgtca ttgaaatggg aaatccaaag accctgctag
    36481 cacgtactat tccagatgcc gaggaagtgg acctcatcct cgttggcgca actggtctca
    36541 acgcctttga acgcctcttg gtcggctctt catctgaata catactccgc catgctaagg
    36601 tcgatttgct ggttgtgaga gaacaagaaa aaaccttata atcacaaaga aaaggagccc
    36661 ctagctcctt tttgtttacg atttatttct ctctttatgg cgttcgtaag ccttgagctg
    36721 gcgctgcagt tcctttttaa tagcaggttc tggagcatat ttttcttccc aattatctgg
    36781 ttttaagatt ttatgggtca ctggatcaaa atgagccttg ccatctggaa aaattttccc
    36841 catattggcc tgatggacaa tatcaaaaat acgttctggg tccaccccca tcaagacaaa
    36901 actgccgtag gtgaagtaaa gcgtgtcaat caaggcatcc acttgcccta tcaaatcttg
    36961 ctgagcaggt gtcttcttgg ctactttacc tgctgcctta tcaagggcct gatgaagttg
    37021 cgatacagct tgaccaaaat cttcttcaga aggactggct gatcgaacaa actccaccaa
    37081 ttcttctatt ttaaaaccag ccctatgggt tgcaccctct aaatcccaag ctcgaggttc
    37141 ttcttgggtt cgttcatcca tcatgtggtg gaaggtcttg accttattga aatgatagtc
    37201 acggctgaca aaaacttttt ctgaagacaa aacaccaaaa tgttccaagg ccttgtggat
    37261 cccatcttgt tgattgctag tggtaatgtg cttggcagct tccttgacac tgctactacc
    37321 atttcccata gcgaccgaca taccgacgcc agccagcatt tcaaggtcat tatctgagtc
    37381 accaaaagcc atgacttggt tgaggtcaaa gccatattct ttcccaactc ggcgaatgcc
    37441 ttctaattta gaatttccct gattgatgac atccgatgca aaaggattgc tacgtgtcaa
    37501 tttcaagtct tcaaaatcag ctgccgcctt ctcagattct tctggtgtca tcagcatcaa
    37561 aacttggtaa ataggttgat tcatcaggtg aagcaggtcc tcttcctttt ggggaacaac
    37621 cttgctgacc atgcgattaa aagactgact caccgtccga gttaaaacag agggaacgaa
    37681 gcgactaatt cgttgggaaa aagaacccag accaaaggac atgattttag aacccaacat
    37741 ggcatccttg gtccctaagg caatctccgt gccctctttt ttagcatagc taattagatg
    37801 gcgcaaatgt aacttggaaa tagggctcgt gaacaagact ctgtctttac taaagatata
    37861 ctggccatta taggttaccg caaaatccag atccaaatcg tccatcaatt ccttaacaaa
    37921 aaaaggtcct cgccctgtcg ctacgccaac tagtacccct tgttctttga caatcttaat
    37981 cgcgtcctta gtggatttca aaacactctt gcgattgttg accaaggttc catcgatatc
    38041 aaaaaaaaca gctttgactt ccatcctatc ccaatctccc cttttgtgat acaatgatta
    38101 taccacattt cagaaagagt gagtaaatca tgcctaagaa aatccttgtt ttacatacgg
    38161 gtggaactat ttccatgcag gccgatgctt ctggcgctgt tgtgacgagt tcagataatc
    38221 ccatgaacca tgtgtccaac ccacttgaag gaatccaagt ccacgccttg gactttttta
    38281 accttccaag tccccatatc aaacccaaac atatgctggt cctctaccag aaaattaaag
    38341 aggaagcaga taactacgat ggagtggtga tcacacacgg aaccgatact ttagaggaaa
    38401 cagcctattt ccttgatacc atggaagttc cccatatgcc tatcgttcta acaggagcca
    38461 tgcgtagctc caatgagctc ggtagtgatg gtgtttataa ttacctaagt gctttacgag
    38521 tggccagcga tgacagggct gctgacaaag gagttttggt cgttatgaac gatgaaatcc
    38581 acgctgccaa gtatgtcacc aaaacacata cgactaatgt cagcaccttc cagactccaa
    38641 cacatggccc cctcggtctc atcatgaaac aggaaatcct ctacttcaaa acagctgaac
    38701 ctcgtgttcg ctttgacctt gatcacatac aaggtttagt ccctatcatc tcggcttatg
    38761 ctggtatgac agatgagctg attgatatgc tggatttaga acacttggac ggtttgatta
    38821 tccaagcctt cggagctggt aatattccca aagaaacggc tcaaaaatta gaaagccttc
    38881 tgcaaaaagg aattccagtc gctctggtat cacgatgctt taacggtatt gccgagcctg
    38941 tttatgccta ccagggtggg ggcgtacagt tgcaaaaagc aggcgttttc tttgttaaag
    39001 aactcaacgc ccaaaaagct cgcttgaaac tcctcatcgc cctcaatgcc ggactaacag
    39061 gacaggcttt gaaaaactat atggaaggct aatactcttc gaaaatctct gcaaaccacg
    39121 tcacgtcgcc ttaccgtatg tatggttact gacttcgtca gtttcatcta caacctcaaa
    39181 aacatgtttt gagctgactt cgtcagttct atctacaacc tcaaaacagt gtgttgcgca
    39241 gcctttaacc agtcgcctag tccgctctat ggtattcatt aagtcaacat ctcttgttta
    39301 agagcacaaa atcaggaaat cttctcgatt ccctgatttt ttctatttac gttttcgtgt
    39361 tgagctacgt tctgtcaaac catgaggtaa gagaacttca cgttcttcca actcttcctt
    39421 atgcataatc ttggtcaaca tacgcatact aatggcacca agatcataaa gaggttgggc
    39481 aatcgttgtc aagtttggac gggtaaagcg tgagatttgt gaatcatcac tagtaataat
    39541 ttcaaaatct tctggcacag aaacaccctt atcagccaaa ccgttcaaga ctcctgctgc
    39601 caactcatca cctgtcacaa ctgctgcagt tgcatttgat gaaatcaaac gctctgctaa
    39661 ggcgtaacca tcatcatagc tatatttaga ttcaaatacc aaaccctcac tataagtgat
    39721 tcctgctttt ttcaaggttt ccttgtagcc aactaaacga accttaccat tgatgtcatc
    39781 cactagcgga ccgctaacga aagcaatacg ctcattttct ttagcaaggt aactcactgc
    39841 atcaattgtt gcttgcttat agtcaatatt gacacttggc aactggtgct caacatcgac
    39901 agttcctgcg agaacaatcg gagtacgtga acgcgaaaat tctgagcgaa ttttatctgt
    39961 caagtgataa cccatataga taatgccatc tacctgcttt gaaaagaggg tattgacaac
    40021 agaaacttct ttctcgttat cttcatcgct attagctagg acaatattgt acttgtacat
    40081 ttctgcaata tcatcaatcc ccttagccaa actcgaaaaa taaccattgg taatatttgg
    40141 aatcacgaca ccgacagtgg ttgtcttttt acttgcaaga ccacgcgcaa ctgcatttgg
    40201 acgataatcc aaacgatcaa ttacctctag cactttttta cgggtattct cttttacatt
    40261 tttattgcca ttgaccacac ggctgaccgt cgccatggaa acacctgctt cacgagcgac
    40321 atcataaatg gttactgtat catctgcatt cattcctttt cctgtccttt ctatctcaca
    40381 cattctttta caagtagagg tactgattga agctctatat ctacttacaa aagtgaagat
    40441 gtgaaaattt cgttttcata tttctactta ttccattcta tcactaattg taaacacttt
    40501 caagtgtttt ttgaagattg attgaaaaaa tttcatagaa aacctaggtt tagctccttg
    40561 ctaccacctt agactaaaca caaaggagga aactaagccc tcctaaagtt atagtaaaat
    40621 gaaataagaa caggataaat cgatcaggac agtcaaatcg atttctaaca atgttttaga
    40681 agtagaggtg tactattcta gtttcaatct actataggta ttgttccatt cactaccgtc
    40741 aattttagca catagccttc ataaaaatat tatatcatca taaccaacca gattcttttg
    40801 cgatattagc tgcctctgtt cgattaccag catccagttt tgaaagaata ttggtgacat
    40861 agtttcgaat agttccgttt gagaggtaaa gctgatctgc gatttcttgg ttggataagc
    40921 cccgggcgat tccctttaag acagcaatct cttgttctgt caacggattg ggacgggtca
    40981 tcaccacttc catcaattca ggcgaatact ccttgcgtcc ttcgaggacg gtgtgcaagg
    41041 tttgcatgag gtctgcaatg cttcgttcct ttaatacata agcatccact ccagccttga
    41101 ccgcacgttc aaaataccca gcacgcttga aggtcgtcac cacaaccacc tttgtttcaa
    41161 gcctttctga tcgtatccac tccaagactt caagacctgt cttaacaggc atttctacgt
    41221 caaggatggc gatatctaca gactcctttt ctaatagttg gattgcttct tgcccattct
    41281 tggcttgaag gacagactcc acatctggtt gaagcgtgag caattgacac atggcatctc
    41341 gcaacatact ttgatcttct gcgactaata ctttcatctt agtttctctc cttataaggt
    41401 agtcgaactt gcacttccgt tggatgtttc tgactgatta cacttacttc tcctgaaaat
    41461 ggaaaaacac gatctcggac tgtatggagc tcatctcctt ttagaaaagc aaagccgcag
    41521 ccatcatcac ttactgttaa aatgagttcc ttctctgtcc gttctaattt taagtaagct
    41581 ttagacgctt tggcatgttt gatgatattg gtcaccaact caagcaaaat catagaagtc
    41641 attgcctcca attcttgagt aaggctagca gtatctagtt ggttatccgt ttccacctca
    41701 attccagcaa tttctaacat ctttttcaca gtctctagtt cggatgtcaa agttctagac
    41761 ttaagatttt ccacaatggt tcgaacttca cgcatcgatt ctttgctaat ttgctgtatt
    41821 tctcttaatt ccttttccac ttgtggataa gcctgcattt gaaatagctg caaggctaga
    41881 tctgtcttga cactaagcat agcaaaggta tgtcccagac tatcatgcaa atcctgaccg
    41941 atacgattgc gctcattttc tgctagcaat agatttatct gggtattttg ctcgacctga
    42001 gcttctttca aatcctcgac aaggcgaatt cgaaccgagc ccaaagtcat caaatcgaca
    42061 aaagtaagaa ttacaagcat atagactaga aactcaactt cgattctctg aaaaatcaaa
    42121 agtcgcccca caacaaggac ttgagcaagg agaaatgtcc atacatgtaa agactttaaa
    42181 ctacgtacgc caaaatgata aattaagaga ttggaaagga aaaagaaaaa ccagatataa
    42241 ttcccagcaa caaaggcggt attcccagct acatagacca gcatgatccc ccagaaaagc
    42301 caagataggc gctggttctt agttattaaa acacccaaat acgtcactac aaatagaata
    42361 tcaaccaata aatgccaagc aggaagctcc ccaatcacta caggtaggat gggaaaaagc
    42421 ataaaaatca aactgaccca aaacatatag tgtatgcttt tcagtctttc aagcattaag
    42481 cattctcctt atgaccttga aggtaaatag tcaaaccaaa taaaactgct gaaaaaacaa
    42541 gtaaataaac tgtggctgat agattgatgc caccctcgtt taagaaggtc ttgagcaact
    42601 ccatcaattg ataggtcggg agacacttac caactgcttg catccagtct ggaaataaag
    42661 aaatgggcat ccagagtccg cctaaaacag ccaatcctag ataaagaaga ttgcccacaa
    42721 cagacatcag ctgactagtt ggtaagagag tcaaggtcaa gccaagtgct acaaaagcaa
    42781 tactccccac aatcagcaaa atcgcagctc caacccattt tactagaggc atgtctacac
    42841 ctcttacaaa atgtccaact gtaaaaacaa ccagaattga gaccaaataa tcaaccagca
    42901 tacttgttat ctttgataga taatattcta ccatatttac agggctatga cgcaatgttt
    42961 tctgccagtt gttcatctta tcggtatgta aaacagctgg aaatgagagg atagttgtcg
    43021 acatcatgga aaaggcagtc atggagatga ggtaatcacg cataaaattc gcgggttcac
    43081 ctggtgtgtc ctgatagata ccagaaaaaa ataaatagaa agccgtcggc atccctaccg
    43141 acaatagata atagattaat tgtcgtttgg tcaataaaaa ttctatctta ctaagtgcta
    43201 tccatcgttt catcttagtt atctcccttc tgcgtctctt caaagattgt attcaacaaa
    43261 ctacgattat taacttcgat ttcttgcatc ctacatcctg cttgggctaa tagttcccag
    43321 aaagcatctg cttctcgagt gactacttgt aaagaatctt gctttagggt ccagttttca
    43381 accaaatttg actgctctac gacttccttg taagctatag gaaggataaa gtgcttttct
    43441 atttcctcgc tacgcatagc tagaggcgtc gtatctcgaa tcaactctcc cttatttaag
    43501 agcaaaatgc ggtctgcagt gtgttccact tcctcaatat aatgggacga atagagaatg
    43561 gtgactcctt gcgcttttaa ctcctggaca atttcccaaa aacgttgacg agttgaggta
    43621 tccatggacg cagtaggctc atctaaaaaa acaatctttg gtcgcccaat taaggtcaag
    43681 atgaaagaaa agagacgctt ttgaccgcct gataattttt ctgccaactg ttctttttgt
    43741 tgcttgacaa actgcaatag ttgatcaatt tcctgattgc tcaaaggatt tggatagata
    43801 cgttgaaaga aagcaatcaa ctctttaact tttaatttct gaacgattac attttcttga
    43861 gggaggtaag ccctagtata gtctaactta gaactcgtca ctggtaaacc ttgaatagat
    43921 acttgaccgc ttgtgaccag tttatctcca agcaaacaat ctaagagtgt tgtctttccg
    43981 gcaccatttg gaccaattaa ggcaacacat tcaccatcgt tgatttcaaa ggagatgttc
    44041 cgcaaaattt ccttgtcttt tattttctta ctcaatttct caactttaac caccgtcatg
    44101 agactctcct ttcaaccact ccattcccat agggaaaacg acgaaaatca taaatccaaa
    44161 ccccaaagca ccatgaatga attggcgaag caaggcttga tcaaaccaat cagtaaacat
    44221 ttccactaac cataccaaga gtgacaggcc gataaagaaa tagatgatcc ctctcttcat
    44281 tcctcaaact cctttttcac atctctgact aatttcaaac cttctctaac aagccaagac
    44341 atgattccaa agccagcaaa gaccttccaa ggaaaatggt agaaatcctc atccaatccc
    44401 gaaaacatga gataggtcat aactcctgct actactaaac tcattgcaat aatcatttta
    44461 tttctcatct cttcttcccc catttcatac tacaattata gtcttttaaa atcagaggag
    44521 gcagaagctt ctgtcactag aaaatatgac aaatgtcata aaaaattctg ttcaaaacaa
    44581 gcaagataca ctatacaata aaacacaatc agaaaaatct aaggcaactt cctcaaaaga
    44641 gatatcaaac tcaattcaca ccataatgta aactaatact tatttaaaat caaaaagagt
    44701 agaaattttt atcagacaaa cacatatata gtgtattgaa tctataacag taggccttaa
    44761 atactaaaat atttctataa attaatttaa ctttcctgat agaactgttc atatcttatt
    44821 tcaattctct atattatacg ttgaacaaaa cccttctatt gctttcttaa aaatttataa
    44881 gagttataaa atctgttaaa tttcaatgtg tatacctaaa ctacggtatt tattgaaaag
    44941 actggggaca aaaagtatac gctgccaaaa tgaattactg aaaatcaaaa aagagaggac
    45001 caaactgatt ccctcttaat gtatataata tctagtttta aaaatacaca ctcacatatc
    45061 tctgt
//
LOCUS       DAVKMU010000013        44219 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKMU010000013 DAVKMU010000000
VERSION     DAVKMU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.013"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..150
                     /locus_tag="VW343_000792"
     CDS             <1..150
                     /locus_tag="VW343_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEU9755384.1"
                     /translation="LSLSSAKEEYINRVLNVLDKEYGGILRYINTELGISSQEIEELK
                     DRYLF"
     gene            1167..2159
                     /locus_tag="VW343_000793"
                     /pseudo
     CDS             1167..2159
                     /locus_tag="VW343_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            2161..2978
                     /locus_tag="VW343_000794"
                     /pseudo
     CDS             2161..2978
                     /locus_tag="VW343_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
     gene            2980..3765
                     /locus_tag="VW343_000795"
     CDS             2980..3765
                     /locus_tag="VW343_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755385.1"
                     /translation="MKKYQRMHLIFIRQYLKQIMEYKADFLVGVVGVFLTQGLNMLFL
                     NILFQHIPLLDGWSFHQVAFIYGFSLIPKGIDHLFFDNLWALGQHLIRKGEFDKYLTR
                     PISPLFHILVETFQIDALGELLVGVLLLLMTITSLTWTWAKVFLFLISIPFATLIYTS
                     LKIVTASIAFWTKQSGAIIYIFYMFNDFAKYPIAIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLKDKDGLFNIGGLILISLIFFTLSLKLWNKGLDAYESAGS"
     gene            complement(4144..5339)
                     /locus_tag="VW343_000796"
                     /pseudo
     CDS             complement(4144..5339)
                     /locus_tag="VW343_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5841..6044
                     /locus_tag="VW343_000797"
     CDS             5841..6044
                     /locus_tag="VW343_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9755386.1"
                     /translation="MYIRLKNLREDKDLSQCQIAQYLHCSQSAYSRIESGKQDIPTSF
                     LKQLAKLYQVTTDYILEMDKFSC"
     gene            6175..6327
                     /locus_tag="VW343_000798"
     CDS             6175..6327
                     /locus_tag="VW343_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755387.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            6494..8287
                     /locus_tag="VW343_000799"
     CDS             6494..8287
                     /locus_tag="VW343_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9755388.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGYDYDVETKQISINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLEHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEAY
                     RQVYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKVS
                     DSIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSNGEIDPAMLTIIFKTGEIQNKDGKQFKSKRKNAKLETDKLCP
                     QNSDEDKKLYSQGTDNTRGVCSVAGSILASQ"
     gene            8392..8979
                     /locus_tag="VW343_000800"
     CDS             8392..8979
                     /locus_tag="VW343_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEU9755389.1"
                     /translation="MGGNPPIKKYSIVDKIVLSTKIKRIIIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYINQEFKSYIDHM
                     LNKEAKIIGFSAGPLLLGEKVYVSPNDNSDHQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELDNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            9261..9509
                     /locus_tag="VW343_000801"
     CDS             9261..9509
                     /locus_tag="VW343_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEU9755390.1"
                     /translation="MCKPISIELCDDEVHSLHEWIDGRDAIDSILTYSENQQYTYGVE
                     AGKILRKIHTIPATEVCEDWEIFFNLKIDDKISNEMIW"
     gene            9685..9938
                     /locus_tag="VW343_000802"
                     /pseudo
     CDS             9685..9938
                     /locus_tag="VW343_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            10066..11295
                     /locus_tag="VW343_000803"
     CDS             10066..11295
                     /locus_tag="VW343_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9755391.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLMMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            11315..14011
                     /gene="ppc"
                     /locus_tag="VW343_000804"
     CDS             11315..14011
                     /gene="ppc"
                     /locus_tag="VW343_000804"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9755392.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    14094..14311
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14424..15428
                     /locus_tag="VW343_000805"
     CDS             14424..15428
                     /locus_tag="VW343_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9755393.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPEFVLKEAGQVIE
                     "
     gene            15497..16363
                     /locus_tag="VW343_000806"
     CDS             15497..16363
                     /locus_tag="VW343_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755394.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            16360..17118
                     /locus_tag="VW343_000807"
     CDS             16360..17118
                     /locus_tag="VW343_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755395.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            17208..18968
                     /gene="dnaG"
                     /locus_tag="VW343_000808"
     CDS             17208..18968
                     /gene="dnaG"
                     /locus_tag="VW343_000808"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9755396.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPYQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRVGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VKEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            18971..20080
                     /gene="rpoD"
                     /locus_tag="VW343_000809"
     CDS             18971..20080
                     /gene="rpoD"
                     /locus_tag="VW343_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9755397.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            20094..20423
                     /locus_tag="VW343_000810"
     CDS             20094..20423
                     /locus_tag="VW343_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9755398.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            20538..21581
                     /gene="cpoA"
                     /locus_tag="VW343_000811"
     CDS             20538..21581
                     /gene="cpoA"
                     /locus_tag="VW343_000811"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9755399.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            21597..22922
                     /locus_tag="VW343_000812"
     CDS             21597..22922
                     /locus_tag="VW343_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9755400.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            22953..23129
                     /locus_tag="VW343_000813"
     CDS             22953..23129
                     /locus_tag="VW343_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9755401.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIVPVATDY"
     gene            23188..23322
                     /locus_tag="VW343_000814"
     CDS             23188..23322
                     /locus_tag="VW343_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9755402.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            23386..24696
                     /gene="obgE"
                     /locus_tag="VW343_000815"
     CDS             23386..24696
                     /gene="obgE"
                     /locus_tag="VW343_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9755403.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            24706..24864
                     /locus_tag="VW343_000816"
     CDS             24706..24864
                     /locus_tag="VW343_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755404.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(24916..26242)
                     /locus_tag="VW343_000817"
                     /pseudo
     CDS             complement(24916..26242)
                     /locus_tag="VW343_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            27345..28604
                     /locus_tag="VW343_000818"
     CDS             27345..28604
                     /locus_tag="VW343_000818"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9755405.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            28620..29171
                     /locus_tag="VW343_000819"
     CDS             28620..29171
                     /locus_tag="VW343_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755406.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALVNYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            29164..30441
                     /locus_tag="VW343_000820"
     CDS             29164..30441
                     /locus_tag="VW343_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9755407.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            30457..31317
                     /locus_tag="VW343_000821"
     CDS             30457..31317
                     /locus_tag="VW343_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9755408.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVIGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            31540..31650
                     /locus_tag="VW343_000822"
                     /pseudo
     CDS             31540..31650
                     /locus_tag="VW343_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
     gene            31696..32500
                     /locus_tag="VW343_000823"
     CDS             join(31696..32064,32066..32500)
                     /locus_tag="VW343_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9755409.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLLRRSQWVEVTLVQSGFTISR
                     TPLSSEDTVMIDATEVKINRPKKELANDSGKKKCHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTVEDKAYNHA
                     LSKDRSKVENIFAKVKTFKMISTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            32692..34983
                     /gene="pcrA"
                     /locus_tag="VW343_000824"
     CDS             32692..34983
                     /gene="pcrA"
                     /locus_tag="VW343_000824"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9755410.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            35026..35706
                     /gene="radC"
                     /locus_tag="VW343_000825"
     CDS             35026..35706
                     /gene="radC"
                     /locus_tag="VW343_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9755411.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(35703..36392)
                     /locus_tag="VW343_000826"
     CDS             complement(35703..36392)
                     /locus_tag="VW343_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9755412.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDSGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(36410..37051)
                     /locus_tag="VW343_000827"
     CDS             complement(36410..37051)
                     /locus_tag="VW343_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9755413.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(37134..37349)
                     /locus_tag="VW343_000828"
     CDS             complement(37134..37349)
                     /locus_tag="VW343_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9755414.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(37357..37704)
                     /locus_tag="VW343_000829"
     CDS             complement(37357..37704)
                     /locus_tag="VW343_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9755415.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(37709..38824)
                     /locus_tag="VW343_000830"
     CDS             complement(37709..38824)
                     /locus_tag="VW343_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9755416.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(38834..39793)
                     /locus_tag="VW343_000831"
     CDS             complement(38834..39793)
                     /locus_tag="VW343_000831"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9755417.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGAPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(39906..40475)
                     /locus_tag="VW343_000832"
     CDS             complement(39906..40475)
                     /locus_tag="VW343_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9755418.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            40603..41274
                     /locus_tag="VW343_000833"
     CDS             40603..41274
                     /locus_tag="VW343_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9755419.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            41258..42076
                     /locus_tag="VW343_000834"
     CDS             41258..42076
                     /locus_tag="VW343_000834"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9755420.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            42073..42969
                     /locus_tag="VW343_000835"
     CDS             42073..42969
                     /locus_tag="VW343_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9755421.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHLLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            43013..43987
                     /gene="pta"
                     /locus_tag="VW343_000836"
     CDS             43013..43987
                     /gene="pta"
                     /locus_tag="VW343_000836"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9755422.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT        13563 a         7571 c         9562 g        13523 t
ORIGIN      
        1 ctttcactca gttcagcaaa agaagaatat attaatcgag tattgaatgt ccttgataaa
       61 gaatatggag gaatacttag atatattaat acagaactag gaataagtag tcaagaaatt
      121 gaggaattga aagatagata tcttttttag aatagtaagt actaaaaatg aatatataac
      181 agtgcgaacg gcggttttgt ttttgtctaa ttctactaat ttaacataag caaaaacgga
      241 ttgttttcta tgtaagtcct taactaaatt gatggaaagc acgttaaatt atgtgcaaaa
      301 tgcgtacaaa atagtgttta tcaaatgtaa aatttcctac ttgtcatcaa agttcaacaa
      361 atgtaggttg tttggttatt tgggtagatt tctggactta tcgttggttg tagagacata
      421 atgttgcagt taaagcttag agaataatct ccgtattttt gttaggcaat ttaaaagtta
      481 agacaaagtg agtttgtctc ttaattcaac tcaccacaaa cacatcacct ttttattata
      541 acttccgacc aatttttttg aagagaaaaa tgaaaaaaca ccaactgtgc agggtagctg
      601 acaaggcttc tagcttccat ttattgaaac caaatcaatc tgttatgaga taatagcagt
      661 tgcagaagct gactactttg agaagttaaa tgaggtttat tatcaagaat acttcatgaa
      721 tatagttata agggaggggg tagttgaaag aattgactca tctgttaaaa taaaaatgga
      781 aataataaca tcataatagt gtgacttagt atttttcagc atcttggaag taggtaaatg
      841 atattaaaat tatactgcat agtaaaggat agtaatgagg aatttaatat cttcctgtca
      901 atatcttcag ttattaaagt atttttatat tgggataact tcgtttgctg aaatctatag
      961 tttatccaat tataaattta tatgttcttc gtcataattc tttgtacttg agtaatttag
     1021 cttacttaca gataaaatac aagatggcat agttttagta gcttttttca tatggtttgt
     1081 aatgatggta aattttctta actttactgt agtatgctac aatttttgtt gatattataa
     1141 cttgagtgaa acgaaaggag tgatttataa aaatgataac agtagagcat cttcagaaaa
     1201 attttgtgaa aacaataaag gaacctggct taaagggagc ttttcgctct ttttttcatc
     1261 ccaagagaca agtctttgaa gctgttaaag atttatcatt tgaggtgccc aaaggacaaa
     1321 ttttaggctt tattggcgca aatggagcgg ggaaatcaac aactatcaag atgttgacgg
     1381 ggattttgaa accaacatca ggtttttgtc gtatcaatgg aaagattcct caagagaacc
     1441 gtcaaggcta tgttaaagat attggtgtag tttttgggca gcgaactcaa ctttggtggg
     1501 atttggcttt gtaggagact tactcggttt taaaggaaat ttatgatgtt cctgatgcgg
     1561 tctttcaaaa acgcatggat tttctaaatg aagttttaga tttgaaggaa tttattcaag
     1621 atcctgtcag aactctatct ctaggtcaac gtatgagggc ggatattgcg gcctccttat
     1681 tgcataatcc gaaagttctt ttcctagatg agcctactat tggtttagat gtttcggtca
     1741 aggataatat tcgacgtgcc attacacaga ttaatcaaga agaagagacg accatacttt
     1801 taacgaccca cgatttaggt gatattgaac agttatgtga tcgtattttt atgattgata
     1861 agggccgaga aatctttgat ggaactgtga atcaactgaa aaagactttc gggaagatga
     1921 agactctctc ttttgaacta catcctgggc aggattatat tgtctctcat tttgaaggac
     1981 tttctgatat ctatgtgacg agacaggaat tgtcacttga tattcagtac gacagctcgc
     2041 agtatcaaac ggctgacatt attcaacaaa ctttgtctga ttttacgatc cgtgatttaa
     2101 agatgacgga tgcgaatatt gaagatatta ttcgtcgctt ctatcgaaag gagctttagg
     2161 atgactaaat tatggaaacg ctataagcct tttgttagcg ctggaatcca agaattaatc
     2221 acgtatcgtg tcaatttttt tctttatcgc attggggatg tgatgggggc ctttgtagca
     2281 ttttacttgt ggaaagctgt gtttgattca tctcaccagt ctctcattca aggttttact
     2341 ttatctgata tgaccttata tatcatcatg agttttgtga cgaatctact gacaaaatca
     2401 gatagttctt ttatgattgg atgggaagta aaggatggct ctattattat gcgactgctt
     2461 cgtccagttc attttgcaat gtcttatctt ttcacagaaa ttgggtctag atggctagtt
     2521 tttgttagtg ttggtctacc atttgtaatt ctgattgcag gtctaaagct cttatcaggt
     2581 gagtccttct tacaaattgt tttaataacc acagtttacc ttcttagctt aatcttagct
     2641 tttttgatta attttttttc aatatttgct ttggtttttc agcttttgtg tttaaaaact
     2701 tatggggatc aaatcttttg aaaaattctt tagttgcctt tttatctgga agtttggttc
     2761 ctctgacatt ttttccaaaa attattgccg aactattatc ttttttgcca ttttcttctt
     2821 taatctatac cccggtgatg gttattatag aaaagtatag catgagtcaa atgattcagg
     2881 cgctctcgct acaattattc tggctgttta ttatgattgc cttatcccaa ctgatttgga
     2941 agtgcgtaca gaattatata actattcaag gaggttagaa tgaagaaata tcaacgcatg
     3001 cacctgattt ttatccgaca atatcttaaa cagattatgg agtataaggc tgatttttta
     3061 gttggtgttg tgggtgtctt tttgacacaa gggttaaaca tgctgtttct aaatattttg
     3121 tttcaacata ttcccttact tgatggctgg agttttcatc aggttgcttt tatttatggt
     3181 ttttctctca ttcctaaggg aattgaccat ctcttttttg ataatctgtg ggctttagga
     3241 caacatttaa ttcgtaaagg agaatttgat aagtatttga ctagacccat tagccctttg
     3301 tttcatattt tagtagaaac gtttcaaatt gatgctcttg gagaattgtt agtaggcgtc
     3361 ttattgctgt taatgacaat aactagtctt acttggactt gggcaaaggt ttttctcttt
     3421 ttaatcagta ttccctttgc aaccttaatt tatacctctt taaaaattgt aacagctagt
     3481 attgcttttt ggactaaaca atctggggca ataatttata ttttttacat gtttaatgat
     3541 tttgcaaaat acccgattgc gatttaccat tcgtttttac gttggttgat tagttttatc
     3601 attccttttg cttttacagc ctattaccca gctagctatt tcttgaaaga caaggatgga
     3661 ttgttcaata ttggtggatt gatccttatc tcattaatat tttttacctt atcattaaaa
     3721 ttatggaaca aaggtttgga tgcatatgag agcgcagggt cttagaagaa gtaaaatcta
     3781 attaagaatt ttgatagaac ttcctattct ttggaaggaa gttttaatga tacattgaga
     3841 gctttgatta ggcttactgt agtttttcaa acaagaaatt tgaccattgt gcaaaaatga
     3901 tacaaaggta tgtaatattt cagtcataac cactcgttca tatcatgagt ggttttttag
     3961 ctcttttata cataccattt gttaagggac atacgatatt ctagctcaaa aggttttctg
     4021 aatgagtatc aaaggaatgt ttagaagata attgcgagct gttttttgaa atttgggcac
     4081 acttaaaaga cacttatctg gtattaaaaa ataaggtgtt acaatgatag gacatagatg
     4141 actttaatga ctttgggtta aagtgtagtc gtaaagtttg ttatgcgtta tgaggtaata
     4201 cattgtccgg atgaggcgat gtatagagac aatcgtatat ggcttggttg aagtcgtctg
     4261 cgattgtctt tttcgtttct cataaaagtc cgcgatatga caaggattgg tatgactagc
     4321 tgaagcaata ttgtggacgc acttatagag aatttttctc gcgtaaggat ttccacgctt
     4381 ggtgatatgt tcttgagcta ggaaattgcc agattcatag tgtctgaggt caatactgat
     4441 aaaggcattg atttggttgg cagaccgaaa gcgacgaatg tctccaattc gccaataatg
     4501 cttgtagctg ttgtttctgc gataccagga atagagagca gaatctcgta ttcaggtaat
     4561 ggttcagcca attcaatcat ttgtgctaag tcctgatcct tgcggtttgt cagtctcaac
     4621 agttcctgtg catgatactt aacctcttct gtcatgggag aggtttttga aatggcaaga
     4681 taggactgtt cggccagctc gctcaacttt tcagccaatc gtccaactct gttctcagaa
     4741 atccgtttag aagttgaatt acgatggctt cttttagctc aactactgat aatttcagcc
     4801 tccaatgctt ggttggaaag atggtcacga ggttccaata ttgttcccct gtaggagtag
     4861 agagaaggtt ctttatttct ggaaaggcaa tttgaagcac cttgtgcagg ctattcttag
     4921 tcctaacgat gtcttctgtc agattctgat agaatcgact gaggtcacgc aaattttgat
     4981 agacttcttc ttgaacataa gttggtttgc ggttaaggat gaattgtgac gaagctagct
     5041 tttcagcgtc aatctgattg gttttacgga ctcgcaaact gtctagttgc ttcttagttt
     5101 taaaggattt agccgtatat aagcatagct attgtcctcc agaaaggcct gaagacgaga
     5161 atagacccct gtgcctcgaa gataatctca ggtgtattta cctatttcaa atcctctaga
     5221 agacgactaa atccgatggc atcattgggc atggtatagc catgaacctt ttcaccattc
     5281 acaagaatag cgacttctga gcttgcctta ctcacatcca ttctgaaaac tgtccgcatc
     5341 atgtttaccc ctttgtcttg aatgactcct tgttttagtg attttatttc caatactcga
     5401 cgtctagtat cccacatact ttgataaaat tcttcctaaa acaggtttct tgccagtttg
     5461 tcaagcgacg tctagcacca tagagagctc tatgacttaa caagacacca ctattttaac
     5521 acaaagaaaa agtaatgagt actctctccc gttggagatt tcctcactac taatcttagt
     5581 atgtttgtca actgtagtga tagacgaata atcaagttct ggagaaggtc agattgggcg
     5641 attacttatt attttcctag cttgtacggt gcttttatcg ctatgaaaaa gtttattcca
     5701 aaatcttttt ttagtaactt atagcttact tactgttata ccaactattt ctaaaatctt
     5761 ataaattatt ttatgcatat tttgcataaa attccaaatt tgataatctt caaattacaa
     5821 tattagtaag aggattatta atgtatatca gattaaagaa tttaagagaa gacaaagatt
     5881 tgtcccagtg tcaaattgca cagtatcttc attgttcaca gtctgcttat tctagaatag
     5941 aaagtggcaa gcaagatatt cctacatcat ttttgaaaca attagctaaa ttatatcagg
     6001 taactacaga ttatatttta gagatggata agtttagttg ttaataaatc ctaagccctt
     6061 tgaaaactga ataaaccgag gtgggacttt cttatttgtc gagcacttta gcttgatacg
     6121 ccgtgatagg agcgataatt attagctata aaatagagtg gttctctatc tgccatgact
     6181 acaaataagg ataatgatac ttctgaacgt tcaggctgcc agcgaaaaag gacagcgggt
     6241 gaaatgccca tgaatgattt aaccagtcat acccacggag gaaattatac atgttacaag
     6301 gaggaaaaag tttggaacaa tcagtaataa agtatactat gcaactatca atgcttaact
     6361 ttttatactc aagaaatatg ttatctaaaa atgagtatga caagattaga aacaaactgc
     6421 gtattaagta cacaaaatag ctgacttttt gatttgaatg cggtaatata gaatcaaaag
     6481 gaggaatttc agtatgaaaa aaaataatgt tgaaataata aaagctgatt ctttagttcg
     6541 tcgaagaggc gacaatgtag aaagacactt aaagagggtt gctgcttatt gcagagttag
     6601 ttccgatagt gaggatcaga aaaacagtta tgattctcaa gtaagacact acaaagaata
     6661 tatttctcag cgttcagatt gggagctggc agatatttat gcagatgaag gtatttcagg
     6721 aacacaagta ggaaagagac aagattttca acgattaatc aatgattgtg caaatggaga
     6781 gatagattat attgtcacaa aagcaattgc tagatttgca aggaataccc tagatacttt
     6841 gaagtatgtc agaatgttaa aggatatgca aattggagta tattttgagg aagaaaacat
     6901 agatacccta acaatggatg gagaattgct tctaactatt ctaagctccg ttgcacagca
     6961 agaagtagag aatacttctg cccatgtgaa aaaaggatta aaaatgaaga tgcaacgtgg
     7021 tgaattggtt ggttttcaag ggtgcttagg gtatgattat gatgtagaaa ccaagcagat
     7081 ttcaattaat aaaaaagaag ccaaaattgt tcgttatatt tttgaaaggt atctagaagg
     7141 tattggagga aaagtaattg caagggaact tgatgaactt ggttacaaat cgccaagagg
     7201 tttagaacac tggaatgata caacagttct aggaataata aagaatgaaa aatataaagg
     7261 tgatattcta atgggaaaaa cttttacagt tgacccaata agcaagagac ggttgagtaa
     7321 ttttggagaa gaagataagt actatatcaa ggataatcat gaaccaataa tatctaaaga
     7381 agattttgaa aaagctcagg agattagatt gcgtcgagca ggaaataaga agacagctgc
     7441 gaatgtaaat gggaagcgtg aacgctattc aaaaatgtat gcttttagta gcatgttgga
     7501 atgtggtttt tgtggttcaa tattatctag aagaagttgg cactgccgtt cagattatcg
     7561 caaagttgtg tggcactgtg ttacatcgat aaagaaagga aagaaatttt gtaagcatag
     7621 taaaggatta gaagagatcg ccattgaggg ggctttcctg gaagcttaca gacaagtcta
     7681 tcattcaaat gaaaatttaa tgacagactt acttgaaacg atagaatctg aattgaatga
     7741 caatagcctt aacaaggaac taaaaaggat tacaaacaaa cttcgaatat tactaaaaaa
     7801 agaggagaat cttgtaaatc taaggctcga aggaaaagtt agcgattcca tatacaatga
     7861 aaagtacaat gaaatttcct cagaaaaaga gtttctagca gaagagaagg taaatattga
     7921 aacaacatta aaatcagaaa tcgatgtaaa gaaaagacta actgagttta agcacttgct
     7981 atcttcgcaa aaaatgctta cggagtttga tcgtgcagtt tttgaaagta ttgttgagaa
     8041 aattattgta ggtggagtta atagcaacgg agaaattgat cctgcaatgt taaccataat
     8101 attcaaaaca ggtgaaattc aaaacaagga tggtaaacaa tttaaaagta aacgtaaaaa
     8161 tgctaaacta gaaacagata aattgtgtcc tcaaaacagt gacgaggata aaaaattgta
     8221 ttctcaagga acagacaaca cacgtggagt gtgtagtgtt gctggttcga tcttagccag
     8281 tcaataaaat gatatgtttt aaaaaaatta taatttcgaa acgtgaataa acttgtttaa
     8341 actttatgta tctttagaaa gtgtgttaac ttgaaagaac aaattttttt gatgggtggg
     8401 aatcccccaa taaagaaata tagtattgtt gataaaattg tattatcaac caagattaaa
     8461 agaattatca tttttacagt ttttcgagaa aattgggaac cgtacatgaa aaagtacacg
     8521 gaagtttttc aaagtcaatt tcctaatcta aacattgatt acttgctcct ggacactgag
     8581 cagattgatc ttgatagcta tctagatgct gacataatta tcatcggtgg tggaaatacg
     8641 gaaaaatata ttgctactta tattaatcag gagttcaaaa gttatatcga tcatatgctt
     8701 aataaagagg caaaaattat aggattttct gcaggacccc tactattagg agaaaaagtc
     8761 tatgtctcac ctaatgataa ttcagatcat cagataaaga taaaaaatgg attaggactc
     8821 tttagtcagt ttttaattag tgtccattat gattcctgga atgataaagc aaataaggat
     8881 agagctgaag aactcgataa tgttcccata attccactaa atgatcattc ttgtcttgta
     8941 ttggataaac ttggaaacat catagagaaa attgactaac tttcataatg taaaaatgga
     9001 tctaggaaat aaaagtgtag acgaactgga taggttacta gtgaaaactg caaaaagtgt
     9061 tgtaaagtga atcggagtgt gcttgatgga ttttagtagt aagatagcca taaataaagg
     9121 ctggtcagat gataaaaaat attgtgtgac agatcaaaat cagtaaaaat atttcttgcg
     9181 tgtttctgat aagtcgaagt tagattataa aaaaatttga atttgatatg atggagaaag
     9241 tagcttctct tggagttccg atgtgtaaac caattagcat tgaactctgc gatgacgaag
     9301 tacattcttt acacgaatgg atagacggaa gagatgcaat agatagcatt ttaacttatt
     9361 cagaaaacca acaatacaca tatggtgtag aagcaggaaa gatacttaga aagattcata
     9421 caattcctgc tacagaagtt tgtgaagatt gggaaatctt ttttaatcta aaaattgatg
     9481 ataaaatctc caacgaaatg atatggtaag tccgaaacgc tcagtgtgta agcttgttgc
     9541 taaaagcgta aaaaccttgg aacgaaagga ataatagact tcctgcgaaa caaaaataca
     9601 atacaataaa actatggatg atgaagcaag taaacaatta agcgatagcc gtttcaagag
     9661 ccttgtaggt gttcagcgca cgagatgtta gctgtgttaa aaacagctta tcaacgcaac
     9721 gtaaacgcgc aaaaggtgga cgaaaaacca aattaagcct agacgatctc ctcatggtaa
     9781 ctattcaata catgcgagaa tagcgcactt atgaacaaat tgcggctgat tttggcattc
     9841 acgaaagcaa cttaatccgt cggagccaat gggttgaagt aactcttgtt caaaatggtt
     9901 ttacgatttc aaattctgcc ctaattatga aaaacagtaa aattcgaagg attgtaaggt
     9961 aagagttttt ttctttctga aaaaatggta taatagcaat caaaactaga aaataaaacg
    10021 gaatttggaa cagatttgtc tgtatcctag tagagtggtg atgctatgaa gattagtaag
    10081 aggcacttat taaattattc catcttgatt ccctacttgc ttttatctat tttgggcttg
    10141 attgtggtct attcgaccac cagtgctatt ttaattgaag aaggcaagag cgccttgcag
    10201 ttggttcgaa accaaggaat cttttggatt gttagtttga tactgattgc cttaatttat
    10261 aaattgagac tagatttttt gagaaatgag cgactaatca ttttagttat attaatagaa
    10321 atgcttttat tgttcttggc tcgttttatt ggtatttccg taaacggggc atacggttgg
    10381 atttcggttg caggaataac tattcagcca gctgagtact taaaaatcat tattatttgg
    10441 tatttagctc accgattctc caaacagcaa gaagaaatag ctacttatga ttttcaagtt
    10501 ttgactcaaa atcaatggct tccccgtgct tttaatgatt ggcgattcgt tctcctagtt
    10561 ctgattggaa gtttgggaat tttccctgat ttaggaaatg cgactatttt agtcttggtt
    10621 tccttgatga tgtatacagt tagtggaatc gcttatcgct ggttttcaac cattctggcg
    10681 ctcgtatctg ccgcttctgt ctttgtcttg accactatca gcctaatcgg tgttgagacc
    10741 ttttcaaaaa ttccagtatt tggctatgta gccaagcgct ttagtgcctt ttttaatcct
    10801 tttgccgatc gtgctgatgc aggtcaccag ttagctaatt cttattttgc catggtcaat
    10861 ggcggttggt ttggtctagg tcttggaaac tcgattgaaa aacgaggtta tttgccagaa
    10921 gctcatacag actttgtctt ttctatcgtg attgaagaat ttggctttgt tggtgccagt
    10981 cttattttag ctctcttgtt tttcatgatt ttgcggatta tcttggtcgg tatccgagcg
    11041 gagaatcctt tcaatgccat ggttgcactc ggtgtcggag ggatgatgtt ggttcaggta
    11101 tttgtcaata tcggagggat ttcgggcttg attccatcta caggagtgac tttccccttc
    11161 ttatcccagg gtggaaatag tcttctagtc ttatcagtgg cagtagcctt tgtcttaaat
    11221 attgatgcca gtgaaaaacg cgctaaattg taccgagaat tggaaaatca accaatgaac
    11281 cttctgttga agtaggataa agaaaggata gtttatgtct cttcaaaaat tagaaaatta
    11341 tagtaataaa agtgttgtgc aagaagaagt cttgattcta acagaattac tggaagatat
    11401 tactaaaaat atgcttgccc cagagacctt tgaaaaaata atacagttga aagaattatc
    11461 aacgcaggaa gattatcaag gtctaaaccg tctagtgact agcttatcaa atgatgaaat
    11521 ggtctatatt tcacgctatt tctctatctt gcctcttttg attaatattt cagaggatgt
    11581 ggatttagct tatgaaatca atcatcaaaa taatattgat caggactatt taggtaaatt
    11641 atctacaacg attaaattgg tagcagaaaa ggaaaatgcc gttgagatcc tagaacactt
    11701 gaatgttgtc cctgttttga cagcccatcc aacacaagtg caacgcaaaa gtatgttgga
    11761 tttaacaaat catattcata gtcttttgcg taaataccgt gatgttaagt tggggttgat
    11821 caataaagat aaatggtaca atgatttgcg tcgttacatc gaaattatca tgcagacaga
    11881 catgattcgt gagaaaaaat taaaagtgac taacgaaatc acgaatgcta tggaatatta
    11941 taacagctcc tttttgaaag ctgtacctca tttgacgacg gagtataagc gcttagcgca
    12001 agcgcatggt ctgaatttaa aacaggctaa accaatcacc atgggtatgt ggataggtgg
    12061 tgaccgtgat ggaaatccat ttgttacagc aaagaccttg aagcagtctg cactcactca
    12121 gtgtgaagtc atcatgaact actatgataa aaagatttac caactttatc gtgaattttc
    12181 tctttcaact agcattgtca acgtcagcaa gcaagtcaga gaaatggctc gtcaatccaa
    12241 ggataactcg atttaccgcg aaaaagagct ttaccgtcgt gccttgtttg atattcaatc
    12301 aaaaattcag gcaactaaaa cctatctgat tgaggatgaa gaagttggga ctcgttatga
    12361 aaccgccaat gatttctaca aggatttgat tgccattcga gattctctac tagaaaataa
    12421 gggcgagtcc ttgatttcag gtgattttgt ggaattattg caggcagtag agatatttgg
    12481 tttttactta gcatcaattg atatgcgaca agactctagc gtctatgaag cctgtgtggc
    12541 agaactcttg aaatcagcag gaattcattc tcgttatagc gagttgagcg aagaagaaaa
    12601 gtgtgacctt ctcttgaaag aattagaaga agatccccga attctttctg cgactcacgc
    12661 agaaaaatca gaattattag caaaagaact agctattttt aagacggctc gtgttttgaa
    12721 agataagttg ggagatgatg tcatccgtca gaccatcatt tcacatgcaa ccagcctttc
    12781 tgatatgcta gaattagcta ttctgttaaa agaagtagga ctggtggata cggaaagggc
    12841 gcgtgttcag attgttcccc tttttgaaac aattgaagac ttggatcatt cagaggaaac
    12901 aatgagaaaa tatctttctc ttagccttgc caaaaaatgg attgactcac gaaataacta
    12961 tcaagaaatc atgcttggct actctgacag taataaagat ggcggttact tgtcatcatg
    13021 ttggaccctc tacaaggctc aacaacaatt gactgctatt ggagatgaat ttggcgttaa
    13081 ggttaccttc ttccatggtc gtggtggtac tgtcggtcgt ggtggtgggc caacctatga
    13141 agccattaca tctcaaccgc tcaagtctat caaggatcgt atccgcttga cggagcaggg
    13201 tgaagtaatt gggaataaat acggtaacaa agacgccgct tactataacc ttgaaatgct
    13261 agtatcggca gctattaacc gtatgattac tcagaagaag agcgatacca ataccccaaa
    13321 tcgttatgaa gccattatgg atcaagtagt ggaccgtagt tacgatatct accgtgattt
    13381 ggtctttggt aatgagcatt tctatgatta tttcttcgag tcaagtccaa tcaaggctat
    13441 ttcaagtttt aatattggtt ctcgtccagc cgctcgtaag actattactg aaatcggtgg
    13501 attgcgtgct atcccttggg tattctcatg gtcacagagt cgtgttatgt tccctggatg
    13561 gtacggggtt ggttcaagct tcaaggaatt tatcaataaa aatccagaga atattgctat
    13621 cttacgagat atgtaccaaa attggccttt cttccaatcg cttctttcaa atgttgatat
    13681 ggttttgtca aaatcaaata tgaatattgc ttttgaatat gctaaacttt gtgaagacga
    13741 gcaagttaag gccatctatg agactatttt aaatgaatgg caagttacta agaacgttat
    13801 cttggctatt gaaggacatg acgaactctt agctgacaat ccatatctaa aagctagtct
    13861 ggattaccgt atgccttact ttaatattct caactatatt cagttagagt tgattaaacg
    13921 ccaacgtcgt ggagaattgt ccagtgatca agaacgattg attcatatca ccatcaacgg
    13981 aattgcgaca ggattgcgta attcaggttg ataattttca agagtgaatg ctaaaagtga
    14041 atatcaaaaa aattctaata gactattgac aagtagttta aaaatgatat aatttaacca
    14101 ttcagaaaag taatcataca aactttttag agagtctgtg gtagctgaaa acagataagt
    14161 ggcaatgatg aaaattgggc tgaatgctat ttagaatttg aaattataaa aattcggtaa
    14221 gcacacctta cagtgcatct cgttattgcg agactgagcg atagggaaat tccctataat
    14281 tgaggtggta ccgcgcatcg acgtcctcac acaagttttt tgtgtgagga tttttttgat
    14341 ggaggttagt atagaaagaa aacgatggcg tcgcttgttt agataagtga aatatgttaa
    14401 aggaaataaa aaggagaaac agaatgaaaa ataaacgttt aattggaatt attgctgcat
    14461 tagcagtctt agtagcagga agcttgattt attcttcaat gaataaatca gaagctcaga
    14521 ataataagga tgagaagaaa ataaccaaga ttggtgtgct tcaatttgtg agccatccat
    14581 cccttgattt gatttataaa gggatccaag atggacttgc agaagaagga tataaagatg
    14641 atcaagttaa aattgatttt atgaactcag aaggtgacca aagtaaggtt gcgacaatga
    14701 gtaaacaatt ggttgcaaat gggaatgacc ttgtggttgg tatcgcaaca ccagcagccc
    14761 aagggttggc tagtgcaaca aaagacctac cggttatcat ggccgctatt acagacccaa
    14821 ttggtgctaa cttggttaaa gatttgaaaa aaccaggtgg caacattaca ggggtatctg
    14881 accacaatcc agctcaacaa caagttgaac tcatcaaggc tctgacaccg aatgtgaaaa
    14941 caatcggagc tctttactca agtagcgaag acaattcaaa aacacaggtc gaagaattta
    15001 aggcttatgc tgaaaaagca ggtctgacag tggaaacatt tgcagttcct tcaacaaatg
    15061 aaattgcctc aactgtcact gttatgacta gcaaggtaga tgctatttgg gttccaattg
    15121 ataacaccat tgcatcagga tttccaacgg ttgtctctag caatcaaagt tctaagaaac
    15181 caatttatcc cagtgcgaca gctatggtag aagtaggtgg tttggcatca gttgtaattg
    15241 accaacatga ccttggtgtg gcaacaggta aaatgattgt gcaagtcttg aaaggtgcaa
    15301 aaccagccga taccccagtc aatgtctttt caactggtaa gtcagtcatc aataaaaaaa
    15361 tagcacaaga actaggtatt actattcctg agtttgttct caaagaagca ggacaagtca
    15421 tcgaataaat aaggtaggga ggaggagact cttcccattt tttaaaaaga tgaatataga
    15481 aaggattaaa taaaatatga ttgtttccat tatttctcaa ggatttgtct gggctattct
    15541 aggtctggga atctttatga catttaggat tttaaacttt ccagatatga cgacagaagg
    15601 ttccttccct cttgggggag ctgttgctgt cactttgata accaaaggcg tgaacccatt
    15661 tttagcgaca cttgttgctg taggagcagg ttgtttggct ggaatggcag caggccttct
    15721 ttatacaaaa gggaagatcc caaccttgct ctcagggatt ttggtgatga cttcttgtca
    15781 ctcaatcatg ctcttgatta tgggacgtgc aaatttaggc ctgcttggaa ccaagcaaat
    15841 tcaggatgtt ttgccttttg attcggattt gaatcaactc ttgacaggtc tcatctttgt
    15901 gagtattgtt attgctctca tgctcttttt cttggacact aaactcggac aagcctatat
    15961 tgctacaggg gataatcctg atatggctag aagtttcggg attcatactg gacacatgga
    16021 gctcatgggc ttggtcttat caaatggtgt gattgccctt gcaggtgccc tcattgctca
    16081 gcaagaaggt tatgccgatg tgtctcgagg gatcggggtt atcgttgtgg ggcttgcaag
    16141 tttgattatt ggagaagtta ttttcaagag tttgagcttg gcagagcgtt tggttactat
    16201 cgttgtaggt tctatcgctt atcaattttt agtgtgggca gttatcgcac ttggctttaa
    16261 tacaagttac cttcgtttat acagtgcctt gattttagca gtctgcctca tgattccaac
    16321 atttaagcaa acaatcttga aaggagccaa gttaagcaaa tgacagcaat tgtagaatta
    16381 aaaaatgcaa ccaaaatcgt taaaaatggc tttgatgaag aaaagattat tttaaatgat
    16441 gtttccttag aaatttttga acaggacttt atcacgattt tgggtggaaa tggtgctggt
    16501 aaatcaaccc tctttaacac tattgcagga accatatcac taaccagtgg aactatccgt
    16561 attttaggtg aggatgttac caagttttca ccagagaagc gcgctaagta tctgtctcga
    16621 gtcttccaag atccaaagat ggggacagct cctcgtatga cagtggcaga gaatctcctg
    16681 attgccaagt ttcgtggtga aaagcgtgga ctatttccga gacgtctggc tagctataag
    16741 gataagtttc aggcgaccat tgaaaaagtg ggaaacggtc ttgagaaaca cttgaataca
    16801 ccgattgagt tcttatctgg tggacaaaga caggctttga gtctcttgat ggcaaccttg
    16861 aagcgacctg aattactctt gttagacgag catactgctg ccctggatcc aaagactagt
    16921 gttgctttga tggaattgac agatgaattt gttaagaaag atcaattgac agcccttatg
    16981 attacccatc atatggaaga tgctctcaaa tatggcaatc gcttgattgt catgaaagaa
    17041 ggacgggtta tccaagattt gaaacaagaa gaaaaagcaa aaatgaaaat ctctgattat
    17101 tatcaactct ttgaataaag tagggaaaat gagtgaaatg gtttactttt tttctgaaat
    17161 aaagtatact atataaagta aactatgata acatggaggt attgtgtatg gttgacaaac
    17221 aagtcattga agaaatcaaa aacaatgcca acattgtgga agtcatagga gatgtgattt
    17281 ctttacaaaa ggcaggacgg aactatctag ggctctgtcc ttttcatggt gaaaaaacac
    17341 cttctttcaa cgttgtagag gacaagcagt tttaccactg ttttggttgt ggtcgctcag
    17401 gtgatgtctt taaattcatc gaggagtacc aaggggttac ctttatggag gctgtccaaa
    17461 tcttaggtca gcgtgtcggg attgaggttg aaaaaccgct ttatagtgaa cagaagccag
    17521 cctcgcctta ccaagctctt tatgatatgc acgaagatgc ggctaaattt taccatgcta
    17581 ttctcatgac aacgactatg ggcgaagagg ccagaaatta cctttatcag cggggtttga
    17641 cagatgaagt gcttaaacat ttttggattg gtttagcacc tccagaacga aactatctct
    17701 atcaacgttt gtctgatcag tatcgtgaag aggatttact ggattcaggc ctgttttatc
    17761 tctcggatgc caatcaattt gtagacacct ttcacaatcg cattatgttt cccctgacaa
    17821 atgaccaagg aaaggtcatt gccttctcag gtcgtatctg gcaaaaaacg gattcacaaa
    17881 cttctaagta taaaaacagc cgatcgactg caatttttaa caaaagttac gaattatatc
    17941 atatggatag ggcaaaaaaa tcttctggaa aagctagtga gatttacctg atggaaggat
    18001 tcatggatgt tattgcagcc tatcgggttg gaatcgaaaa tgctgtggcg tcgatgggaa
    18061 cggccttgag tcgagagcat gttgagcatc tgaaaaggtt aaccaagaaa ttggttcttg
    18121 tttacgatgg agataaggct gggcaagccg cgatattgaa agcattggat gaaattggtg
    18181 atatgcctgt gcaaatcgtc agcatgcctg ataacttgga tcctgatgag tatctacaaa
    18241 aaaatggtcc agaagacttg gcctatctat taacgaaaac tcgtattagt ccgattgagt
    18301 tctacattca tcagtacaaa cctgaaaaca gtgaaaatct gcaggctcag attgagttta
    18361 ttgaaaaaat agctcccttg attgttaaag aaaagtccat cgctgctcaa aacagctata
    18421 ttcatatttt agctgacagt ctggcgtcct ttgattatgc ccagattgag cagattgtta
    18481 atgagagtcg tcaggtgcaa aggcagaatc gcatggaaag aatttccaga ccgacgccaa
    18541 tcaccatgcc tgtcaccaag cagttatcgg ctattatgag ggcagaagcc catctactct
    18601 atcggatgat ggaatcccct cttgttttga acgattaccg tttgcgagaa gactttgcat
    18661 ttgctacacc tgaatttcag gtcttatatg acttgcttgg ccagtatgga aatcttcctc
    18721 cagaagtttt agcagagcag acagaggaag ttgaaagagc ttggtaccaa gttttagctc
    18781 aggatttgcc tgctgagata tcgccgcagg aacttagtga agtagagatg actcgaaaca
    18841 aggctctctt gaatcaggac aatatgagaa tcaaaaagaa ggtgcaggaa gctagccatg
    18901 taggagatac agatacagcc ctagaagaat tggaacgttt aatttcccaa aagagaagaa
    18961 tggagtaata atggcaacaa aacaaaaaga agtaacaaca tttgacgtac aggtagcaga
    19021 atttatccgt aatcataagc aaaaagggac agcaacagat gatgaaatca atgcaagtct
    19081 ggttattcct tttaccttgg acgctgatgg gattgaagat ctcttgcaac ggattcagga
    19141 tgcaggcatt tctatcacag ataacgaagg aaatccaagt gcgcgtgttc ttagcaatga
    19201 agaagaacca gaactcagcg atgaggactt gattgggtca acttctgcta aggtcaatga
    19261 ccctgtccgt atgtacttga aagaaatagg ggtcgttcct ctcttgacca atgaagagga
    19321 gaaagagttg gcactggctg ttgaagctgg cgatatcgaa gccaaacaac gtcttgcgga
    19381 agccaatctt cgtttggttg tttccattgc caaacgctat gtcggtcgtg gcatgcagtt
    19441 ccttgacttg attcaagaag gaaatatggg cttgatgaag gcggttgaca agtttgacta
    19501 ttctaaaggg ttcaagtttt caacttatgc aacttggtgg attcgtcagg ctatcactcg
    19561 tgctattgcg gaccaagctc gtaccatccg tatcccagtt cacatggttg aaactatcaa
    19621 taaattggtt cgtgaacagc ggaatctcct tcaagaattg gggcaagatc cgacaccaga
    19681 acagattgct gaacgaatgg atatgacacc tgataaggtt cgtgaaatct tgaagattgc
    19741 ccaagaacca gtatctcttg aaactcctat cggtgaagag gacgatagcc accttggaga
    19801 ctttatcgaa gatgaagtga ttgaaaatcc agtggattat acgactcgta tcgtcttgcg
    19861 tgagcaattg gatgaaatct tagatactct tacagaccgt gaagaaaatg ttctgcgtct
    19921 acgttttgga ctagatgatg gaaaaatgcg cacacttgaa gatgtgggga aagtctttaa
    19981 cgtaactcgt gagcgtatcc gtcagattga agcaaaggct ttgagaaaac tacgccaacc
    20041 aagtcgcagc aaaccgcttc gtgattttat tgaagactaa gagtgaggaa aatatggctt
    20101 atacagaaga gcaaattgaa aacatcaaaa cacggatttt aacagccttg gaagaagtca
    20161 tcgaccctga gttgggaatc gatattgtca atcttggttt gatctatgag attcgttttg
    20221 acggtgacac agggcaaaca gagattgaca tgactttgac aactatgggt tgtcccttag
    20281 cagacctttt gacagaccag atttatgatg ctatgataga ggttccagaa gtaacggata
    20341 ctgaggttaa attagtctgg tatccagcct ggacagttga aaaaatgagt cgctatgccc
    20401 gcattgccct aggcattaag taaacatata aaagcatgtg agagactgtt ggaaaagcga
    20461 ggaaatttcc cctcttttcc tctagtctct cctttctttt gctgatttta ttcaaagaaa
    20521 atgatataat agtagttatg gagaaaaaga aattacgcat caatatgttg agttcaagtg
    20581 agaaagtagc aggacaggga gtttcaggtg cttaccgtga attagttcgt cttcttcacc
    20641 gtgctgccaa ggaccaattg attgttacag aaaatcttcc gatcgaggca gatgtgactc
    20701 actttcatac gattgatttt ccctattatt tatcaacctt ccaaaagaaa cgctcaggga
    20761 gaaagattgg ctatgtgcat ttcttgccag ctacacttga gggaagtttg aaaattccat
    20821 ttttcttaaa gggaattgtg aaacgctatg tattttcttt ttacaaccgg atggagcact
    20881 tggttgtggt caatcctatg tttattgagg atttggtagc agctggtatt ccacgtgaaa
    20941 aagtgaccta tattcctaac tttgtcaaca aggaaaaatg gcatcctcta ccacaagaag
    21001 aggtagtcag actgcgcaca gatcttggtc ttagtgacaa tcagtttatc gtagtaggtg
    21061 ctgggcaagt tcagaaacgt aaagggattg atgactttat ccgtctggct gaggaattgc
    21121 ctcagattac ctttatctgg gctggtggct tctcttttgg tggtatgaca gatggttatg
    21181 aacactataa gaaaattatg gaaaatcccc ctaaaaattt gatttttcca ggcattgtat
    21241 cgccagagcg gatgcgcgaa ttgtatgctc tagcggatct tttcttgttg cctagttaca
    21301 atgagctctt tcctatgact attttagaag ctgcgagttg tgaggctcct attatgttgc
    21361 gtgatttaga tctctataag gtgattttgg agggaaatta tcgggcgaca gcgggtagag
    21421 aagagatgaa agaggctatt ttggaatatc aagcaaatcc tgctgcctta aaagatctca
    21481 aagaaaaggc taagaatatt tccagagagt attctgaaga gcatctgtta caaatctggt
    21541 tggactttta tgagaaacaa gccgctttag ggagaaagta aaaagtgagg taatctatgc
    21601 gaattggttt atttacagat acctattttc ctcaggtttc tggtgttgcg accagtattc
    21661 gaaccttgaa aacagaactt gaaaagcagg gacatgctgt ttttatcttt acgacgacag
    21721 ataaggatgt caatcgctac gaagattggc aaattatccg cattccaagt gttcctttct
    21781 ttgcttttaa ggatcgtcgc tttgcctacc gaggttttag caaggcactt gaaattgcta
    21841 aacagtatca gctagatatt atccatactc agacagaatt ttctcttggc ctgttgggga
    21901 tttggattgc gcgtgaattg aaaattccag tcatccatac ctatcacacc cagtatgaag
    21961 actatgtcca ttatattgct aaggggatgt tgatccgacc gagtatggtc aagtatctgg
    22021 ttagaggttt cctgcatgat gtggatgggg ttatttgccc tagtgagatt gtccgtgact
    22081 tgctatctga ttataaggtc aaggttgaaa aacgggtcat tcctactggg attgaattag
    22141 ccaagtttga gcgtccggaa atcaagcagg aaaatttgaa agaactgcgt agtaaactag
    22201 ggattcaaga tggtgaaaag acgttgctta gtctttcgag aatctcctat gaaaaaaata
    22261 ttcaagcagt tttagtagcc tttgctgatg ttctgaaaga ggaagacaag gttaaactgg
    22321 tagtagctgg ggatggccct tatctgaatg acctcaaaga gcaagcccag aacctagaga
    22381 ttcaagactc agtcatcttt acagggatga ttgctcctag tgagacggct ctttactata
    22441 aagcggcgga ttttttcatt tcggcatcga caagcgaaac gcaaggtttg acctacttgg
    22501 aaagcttagc cagtggaaca cctgtcattg ctcacggaaa tccttatttg aacaacctca
    22561 tcagtgataa aatgtttgga accttgtact atggagaaca tgatttggct ggtgctattt
    22621 tggaagccct gattgcaaca ccagacatga acgagcatac cttatcagag aaattgtatg
    22681 agatttcagc tgagaacttt gggaaacgag tgcatgagtt ttatctggat gccattattt
    22741 caaataactt ccagaaagat ttggctaaag atgatacggt cagtcagcgt atctttaaga
    22801 cagttttgta tcttcagcaa caggtggttg ctgtacctgt aaaaggatct agacgcatgt
    22861 tgaaggcttc aaaaacacag ttgatcagta tgagagacta ttggaaagac catgaagaat
    22921 agaaagagga acagctatga aaaaaacaat taatgagaag cggtcgtgat aaaaagattg
    22981 cgggtgtttg tgctggggtg gcccattatc tggatatgga tccgactatc gttcaagtca
    23041 tttggggtgt tcttacttgc tgttacggag ctggaattgt agcttacatt attttatgga
    23101 ttatcgtacc agtagcaact gactattgaa aactagctca attcatgcta agataataga
    23161 aaatagaatg taacgaagga gagaaaaatg gcatttggag ataatggaaa tcgtaaaaaa
    23221 actatgtttg agaaaataac cttgtttatc gtgattatca tgctagtagc aagtttattg
    23281 ggaatttttg caactgcaat tggtgccctc agtaatctat aaaatagatt caagaaaatt
    23341 tagtgactgg gatttcccag ccctttttta aagtgagaag aaataatgag tatgttttta
    23401 gatacagcta agattaaggt caaggctggt aatggtggcg atggtatggt tgcctttcgt
    23461 cgtgaaaaat atgtccctaa tggaggccct tggggtggtg atggtggtcg tggaggcaat
    23521 gtggtcttcg ttgtagacga aggattacgt accttgatgg atttccgcta caatcgtcat
    23581 ttcaaggctg attctggtga aaaagggatg accaaaggga tgcatggtcg tggtgctgag
    23641 gaccttagag ttcgagtacc acaaggtacg actgttcgtg atgcggagac tggcaaggtt
    23701 ttaacagatt tgattgaaca tgggcaagaa tttatcgttg cccacggtgg tcgtggtgga
    23761 cgtggaaata ttcgtttcgc gacaccaaaa aatcctgcac cggaaatctc tgaaaatgga
    23821 gaaccaggtc aggaacgtga gttacaattg gaactaaaaa tcttggcaga tgtcggttta
    23881 gtaggattcc catctgtagg gaagtcaaca cttttaagtg ttattacctc agctaagcct
    23941 aaaattggtg cctaccactt taccactatt gtaccaaatt taggtatggt tcgcacccaa
    24001 tcaggtgaat cctttgcagt agccgacttg ccaggtttga ttgaaggggc tagtcaaggt
    24061 gttggtttgg gaactcagtt tctccgtcac atcgagcgta cacgtgttat ccttcacatc
    24121 attgatatgt cagctagcga aggccgtgat ccatatgagg attacctagc tatcaataaa
    24181 gagctggagt cttacaatct tcgcctcatg gagcgtccac agattattgt agctaataag
    24241 atggatatgc ctgagagtca ggaaaatctt gaagaattta agaaaaaatt gtctgaaaat
    24301 tatgatgaat ttgaagagtt accagctatc ttcccaattt ctggattgac caagcaaggt
    24361 ctggcaacac ttttagatgc tacagctgaa ttgttagaca agacaccaga atttttgctc
    24421 tacgacgagt ccgatatgga agaagaagct tactatggat ttgacgaaga agaaaaagcc
    24481 tttgaaatta gtcgtgatga cgatgcgaca tgggtacttt ctggtgaaaa actcatgaaa
    24541 ctctttaata tgaccaactt tgatcgtgat gaatctgtca tgaaatttgc ccgtcagctt
    24601 cgtggtatgg gggttgatga agcccttcgt gcgcgtggag ctaaagatgg ggatttggtc
    24661 cgcattggta aatttgagtt tgaatttgta gactaggaga ctggtatggg agataaaccg
    24721 atatctttcc gagatgcgga tggtaatttt gtttccgccg cagacgtttg gaatgaaaag
    24781 aaattggaag aactatttaa tcgtctcaat ccaaatcgtg ccttgagatt ggcacgaact
    24841 aaaaaggaaa atccatctca gtaaagaagc taaaaaatcc cggctctttg tcaactgtag
    24901 tgggttgaag aaaagctaag ctcgagaaag gacaaatttt gtcctttctt ttttgatatt
    24961 cagagcgatg aaaatccgtt ttttgaagtt ttcaaagttt cgaaaaccaa aggcattgcg
    25021 cttgataagt ttgatgagat tattggtcgc ttccagtttg gcattagaat agtgtagttg
    25081 aagggcgttg acaatctttt ctttatcttt gaggaaggtt ttaaagacag tctgaaaaat
    25141 aggatgaacc tgcttaagat tgtcctcaat aagtccgaaa agtttctctg gttccttatt
    25201 ctggaagtga aaaagcaaga gctgatagag ctgatagtga tgtttcaagt cttccgaata
    25261 gctcaaaagc ttgtttaaaa tctctttatt ggttaaatgc atacgaaaag taggacgata
    25321 aaaacgttta tcactcagtt tacggctatc ctgttgaatg agtttccagt agcgcttgat
    25381 agccttgtat tcatgggatt ttcgatgaaa ctgattcatg atttggacac gcacacgact
    25441 catagcacgg ctaagatgtt gtacaatgtg aaagcgatca agaacgattt tagcattcgg
    25501 gagtgaaaca gtctgggaga ctgtttcagc ctgagcctag aaatttgaaa gcgaagctgt
    25561 ttagccaagc catagtaagg gctaaacata tccatggtaa tgattttgac gcgacatcgg
    25621 acggctctat catatttaag aaagtgatct cggatgacag cttgtgttct gccttcaaga
    25681 acagtgatga tattgagatt gttaaaatct tgcgcaataa agctcatctc catctcccga
    25741 ttgaaacagt cactccccgg actgtttcaa cgtcccagga cataatctca ggaagacaag
    25801 aaaaatcatg tttaaagtga aaatcattga gcttacgaat aacagttgaa gttgaaatgg
    25861 ccagctgatg agcaatatcg gtcatagaaa tcttctcaat caacttttgc gcaatttttt
    25921 ggttgataat acgaggaatt tggtgatttt tcttgacgat agaagtttca gcgaccatca
    25981 tttttgaaca gtgatagcac ttgaatcgac gctttctaag gagaattcta gtaggcatac
    26041 cagttgtctc aaggtaagga atcttagacg gtttttgaaa gtcatatttc ttcaattggt
    26101 ttccgcactc agggcaagat ggtgcgtcgt agtccagttt ggcgatgatt tccttgtgtg
    26161 tatctttatt gatgatgtct aaaatctgga tattagggtc tttaatgtct agtaattttg
    26221 tgataaaatg taattgtccc atatgaatct ttctaatgag ttgttttgtc gcttttcatt
    26281 atagatctta tgggattttg tggtacgaca ggcatgtata gcaaactgaa tctggaatag
    26341 cacagcatat cttctaaaat attgttataa attgatttga atttcaaaat taaattgttt
    26401 gattcttatt tcaatttgtt atagtatatc tgatgtcaaa gttctcggcg aatcaaatag
    26461 cgattcccaa gcctgactat cgtgaggtag cggattaaaa tggtctgggg atagaccgtt
    26521 ttaagtctga cgctggaaat aagaattgtc agaagaaggg atagcgaaat cgtggctcta
    26581 cgaacaggaa cgtgataata aggcgtatat agcggataag agggcctcaa actctaaagt
    26641 ccaaaaaggt agtcgtaacc tatatgcgta aatcacgaga gtaattgaat tcgtactaag
    26701 attttctatt ttcactgtaa ccttttaacg tcctcatatc ttgtatacac gaggaaagat
    26761 gtacgactta tcccgtgagg tctatcacta taaagagaaa acgacagata gaagtgatcc
    26821 tgagtcacgg ttatctttaa aatagattgt ctgataggat gctagcctga aaacgtcttg
    26881 ttcatgaaat ataggacggt atgtataaaa cgcttctgtg aactgagaga agggggagaa
    26941 gttcttgcta aaatttagtt gaacagccgt attccgatat ttagataaga gatctagtct
    27001 tagctcctac tcggtttcag gggataaaaa aggggcaata gcgattcgag aaagattata
    27061 ctcttcgaaa atctcttcaa atcacgtcaa tatcgccttg ccgtacgtgt aggatactga
    27121 ctacgtcagt tccatctaca acctcaaaac agtgttttga gcaacctgcg gctagtttcc
    27181 tagtgtgatc tttgattttt attgagtatt agtaattcag ttactaactc gtcaactctg
    27241 atttatccaa taaaattgaa aaggatggaa aaaaggataa atttatgata tactttattt
    27301 tgaagacctt attagaaatc ttgaaagagt attgaaaact tagaatgaga aaaattgtta
    27361 tcaatggtgg attaccactg caaggtgaaa ttactattag tggtgctaaa aatagtgttg
    27421 tggccttaat tccagctatt atattggctg atgatgtggt gactttggat tgtgttccag
    27481 atatttcgga tgtagccagt cttgtcgaaa tcatggaatt gatgggagct actgttaagc
    27541 gttatgacga tgtcttggag attgatccaa gaggtgttca aaatattcca atgccttatg
    27601 gtaaaattaa cagtcttcgt gcatcttatt atttttatgg gagcctctta ggccgttttg
    27661 gtgaagcgac agttggtcta ccgggaggat gtgatcttgg tcctcgtccg attgacttac
    27721 accttaaggc gtttgaagct atgggtgcca ctgctagcta cgagggagat aacatgaagt
    27781 tatctgctaa agatacagga cttcatggtg caagtattta catggatacg gttagtgtgg
    27841 gagcaacgat taatacgatg attgctgcgg ttaaagcaaa tggtcgtact attattgaaa
    27901 atgcagcccg tgaacctgag attattgatg tagctactct cttgaataat atgggtgccc
    27961 atatccgtgg ggcaggaact aatatcatca ttattgatgg tgttgaaaga ttacatggga
    28021 cacgtcatca ggtgattcca gaccgcattg aagctggaac atatatatct ttagctgctg
    28081 cagttggtaa aggaattcgt ataaataatg ttctttacga acacctggaa gggtttattg
    28141 ctaagttgga agaaatggga gtgagaatga ctgtatctga agacagcatt tttgtcgagg
    28201 aacagtctaa tttgaaagca atcaatatta agacagctcc ttacccaggc tttgcaactg
    28261 atttgcaaca accgcttacc cctcttttac taagagcgaa tggtcgtggt acaattgtcg
    28321 atacgattta cgaaaaacgt gtaaatcatg tttttgaact agcaaagatg gatgcggata
    28381 tttcgacaac aaatggtcat attttgtaca cgggtggacg tgatttacgt ggggccagtg
    28441 ttaaagcgac cgacttaaga gctggggctg cactagtcat tgctgggctt atggctgaag
    28501 gtaaaactga aattaccaat atcgagttta tcttacgtgg ttattctgat attatcgaaa
    28561 aattacgtaa tttaggagcg gatattagac ttgttgagga ttaaaccgta gaggtgttta
    28621 tgaatatttg gaccaaatta gcaatgtttt ctttttttga aacggatcgc ttgtatttgc
    28681 gtcctttctt ttttagtgat agtcaggact tccgcgagat agcttcaaat ccagaaaatc
    28741 ttcaatttat tttcccaacg caggcaagtc tggaagaaag tcaatatgca ctggtcaatt
    28801 actttatgaa gtcccctttg ggagtgtggg caatttgtga ccagaaaaat caacaaatga
    28861 ttggttctat taaatttgag aagttagatg aaatcaaaaa agaagctgag cttggctatt
    28921 ttttgagaaa agatgcttgg tcgcaaggat ttatgacaga ggttgttaga aaaatttgtc
    28981 agctttcttt tgaggaattt ggcttaaaac aattatctat cattacccac cttgaaaatg
    29041 aagctagcca aagagttgct cttaagtctg gatttagttt gttccgtcag tttaagggaa
    29101 gtgatcgtta cacaagaaaa atgcgggatt atcttgaatt tcggtatgta aaaggagagt
    29161 tcaatgagta agcatcagga aattctaagc tatttggagg aattaccagt aggtaaaagg
    29221 gtcagtgttc gtagcatttc gaatcatcta ggagttagtg atggaacagc ctatcgggct
    29281 attaaagaag ctgaaaaccg tggaattgtg gagacccgtc ctagaagtgg aacaattcgt
    29341 gttaaatccc agaaagttgc tatagagaga ttaacgtttg ctgaaattgc agaagtgact
    29401 tcttctgagg ttctggctgg gcaagaaggt ttagagagag aatttagtaa gttttcaatt
    29461 ggtgccatga ctgaacaaaa tatcttgtct taccttcatg atggggggct cttgattgtc
    29521 ggagaccgaa cccgtattca gttgctagcc ttggaaaatg aaaatgcagt tctggttaca
    29581 gggggatttc aggttcatga tgatgtgctt aaactggcca atcaaaaagg gattcctgtt
    29641 ctaagaagta agcatgatac ctttaccgtc gcgaccatga tcaataaagc cttgtcaaat
    29701 gtccaaatca agactgatat tctgacagtt gagaaacttt atcgccctag tcatgagtat
    29761 ggttttctga gagagacaga tacagttaaa gattatttgg acttggttcg taagaatcgt
    29821 agcagccgtt tccctgttat caatcaacat caggtcgttg ttggcgttgt aaccatgaga
    29881 gacgctggtg ataaatcacc aagcacgaca attgataagg ttatgtctcg tagtctattt
    29941 ttggttggat tatcgacaaa tattgccaat gtgagtcaac ggatgatcgc agaagacttt
    30001 gaaatggtac cagttgttcg aagcaatcaa actttgcttg gcgttgtgac gcgacgagat
    30061 gtcatggaga agatgagccg ttcccaagtt tcggctctac caactttttc tgagcagatt
    30121 ggacaaaagc tctcttatca ccatgatgaa gtagtcatta cagtggaacc ctttatgcta
    30181 gaaaaaaatg gagttttggc taatggtgta ttggcagaaa ttctgaccca catgacccaa
    30241 gatttagttg ttaatagtgg tcgcaatctc attatcgagc agatgctgat ctactttttg
    30301 caggctgttc agatagatga tatattgcgc attcaggcac ggattattca tcatacgaga
    30361 cggtcagcta taattgatta cgatatttat catggtcacc agattgtttc aaaagcaaat
    30421 gtgactgtta aaattaatta gaaactagga gaaaagatga taacattaaa atcagctcgt
    30481 gaaatcgaag ctatggacaa ggctggtgat tttctagcaa gtattcatat aggcttacgt
    30541 gatttgatta agccaggcgt agatatgtgg gaagttgaag aatatgtccg ccgtcgttgt
    30601 aaagaagaaa atttccttcc acttcagatt ggggttgacg gtgccatgat ggactatcct
    30661 tatgctacct gttgctctct taacgatgaa gtggctcacg ctttccctcg tcattatatc
    30721 ttgaaagatg gtgatttgct caaagttgat atggttttgg gaggtcccat tgctaaatct
    30781 gacctgaatg tctcaaaatt aaacttcaac aatgttgaac aaatgaaaaa atacactcag
    30841 agctattctg gtggtttagc agactcatgt tgggcttatg ctgttggtac accgtccgaa
    30901 gaagtcaaaa acttgatgga tgtaaccaaa gaagctatgt acaagggtat tgagcaagct
    30961 gttattggaa atcgtatcgg tgatatcggt gcggctattc aagaatacgc tgaaagtcgt
    31021 ggttacggtg tagtgcgtga tttggttggt catggtgttg gcccaactat gcacgaagaa
    31081 ccaatggttc ctaactatgg tattgcaggt cgtggactcc gtcttcgtga aggaatggtc
    31141 ttaaccattg aaccaatgat caatacaggc gattgggaaa ttgatacaga tatgaaaact
    31201 ggttgggcgc ataagaccat tgacggtgga ttgtcatgtc agtatgaaca ccaatttgtc
    31261 attacgaaag atggacctgt tatcttgact agccaaggtg aagaaggaac ttattaataa
    31321 aaagtgaaaa gactactgga agtttattat gaaaaaatcc agtagatctt ttcataataa
    31381 aacgcattgt atcaagtgtt aggggctgat atcatgcgtt tttctgcttt taagattttt
    31441 tccaactctg tttgtaagcg catcataaca aagggtctag gattcagggc tctcctccta
    31501 tatactatta gtaaagtaaa actaagggag gatattttag tgtcgcagtc tattgttcct
    31561 gtagagattc cacaatattg tcgttttgat tctaaaaaga gaaatggaat tctgtttaat
    31621 gttcgtattg ccaatcttaa atttaccttt tttagattag acttcctgcg aaacaaaata
    31681 tggtatagta gttccatgaa ttatgaagca agtaaacaac taactgatgc acgatttaag
    31741 cgtcttgttg gtgttcagcg taccactttt gaagagatgt tagctgtatt aaaaacagct
    31801 tatcaactta aacacgcaaa aggtggacga aaacctaaat taagcctaga agaccttctt
    31861 atggccactc ttcaatatgt gcgagaatat cgaacttatg aacaaattgc ggctgatttt
    31921 ggtattcacg aaagcaactt actccgtcgg agccaatggg ttgaagtaac tcttgttcaa
    31981 agtggtttta cgatttcaag aactcctctc agttctgagg acacggtaat gattgatgcg
    32041 acggaagtaa aaatcaatcg ccctaaaaaa agaattagcg aatgattctg gtaaaaagaa
    32101 atgccacgct atgaaggctc aagcgattgt cacaagtcaa gggagaattg tttctttgga
    32161 tatcgctgtg aactattgtc atgatatgaa gttgttcaaa atgagtcgca gaaatatcgg
    32221 acaagctgga aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga tatatcctca
    32281 agcacaaact ccacgtaaat ccagcaaact caagccgcta acagttgaag ataaagccta
    32341 taaccatgcg ctatccaagg atagaagcaa ggttgagaac atctttgcca aagtaaaaac
    32401 gtttaaaatg atttcaacaa cttatcgaaa tcatcgtaaa cgtttcggat tacgaatgaa
    32461 tttgagtgct ggtattatca atcatgaact aggattctag ttttgcagga agtctattga
    32521 ggtattgagc tagtttatga aaaaattggg tgaaaaagtc gagtgtttta gaaacccaca
    32581 gtgtagtatt ctagtttcaa tccactatat tttgctactc cccgtaaagt ttctattttc
    32641 cctgatttct gatataatag aaatattgac ttcaagagta aggaagagaa gatgaacgca
    32701 ttattaaatg gaatgaatga ccgtcaggct gaggcggtgc aaacgacaga aggtcccttg
    32761 ctaatcatgg caggggctgg ttctggaaag actcgtgttt tgacccaccg tatcgcttat
    32821 ttgattgatg aaaagctggt caatccttgg aatatcttgg ccattacctt taccaacaag
    32881 gctgcgcgtg agatgaaaga gcgtgcttat agcctcaatc cagcgactca ggactgtctg
    32941 attgcgacct tccactccat gtgtgtgcgt attttgcgtc gcgatgcgga ccatattggc
    33001 tacaatcgta attttacaat tgtggatcct ggtgaacagc gaacgctcat gaaacgtatt
    33061 ctcaaacagt tgaacttgga tcctaaaaaa tggaatgaac gaactatttt ggggaccatt
    33121 tccaatgcta agaatgattt gattgatgat gttgcttatg ctgcccaagc tggcgatatg
    33181 tatacgcaaa ttgtggccca gtgttataca gcctatcaaa aagaacttcg tcagtctgaa
    33241 tccgttgact ttgatgattt gattatgctg accttgcgtc tctttgatca aaatcctgat
    33301 gttttgacct actaccagca aaaattccaa tacatccacg ttgatgagta ccaagatacc
    33361 aaccacgctc agtaccaatt ggtcaaactc ttggcttccc gttttaaaaa tatctgtgtg
    33421 gttggggatg cggaccagtc tatctacggt tggcgtggtg ctgatatgca gaatatcttg
    33481 gactttgaaa aggattaccc caaagccaag gttgttttgt tggaggaaaa ttaccgctca
    33541 accaaaacca ttctccaagc ggccaacgag gttattaaaa ataataaaaa tcgccgtcct
    33601 aaaaatctct ggactcaaaa cgctgatggg gagcaaatcg tttactatcg tgccgatgat
    33661 gagctggatg aggctgtatt tgtagccaga accatcgatg aacttagtcg cagtcaaaac
    33721 ttccttcata aggattttgc agttctctat cggactaatg cccagtcccg tacaattgag
    33781 gaagccctgc tcaagtctaa cattccttat accatggttg gcggaaccaa attctacagc
    33841 cgtaaggaaa ttcgcgatat tattgcttat ctcaacctta ttgctaattt gagtgacaat
    33901 attagttttg agcgtattat caacgagcct aaacgtggaa ttggtctagg tacagttgag
    33961 aaaatccgtg attttgcaaa tttgcaaaat atgtctatgc tggatgcttc tgctaatatt
    34021 atgttgtctg gtatcaaggg taaggcagcc caatctatct gggattttgc caatatgatg
    34081 cttgatttgc gggagcagct agaccactta agcattacag agttggttga gtccgtccta
    34141 gaaaaaacag gttatgtcga tattcttaac gcccaagcga ctctagaaag caaggcacgg
    34201 gttgaaaata tcgaagagtt tctttctgtt acgaagaact ttgatgacac cactgatgtg
    34261 acagaagagg aaactggtct ggacaaactg agtcgtttct taaatgactt ggctttgatt
    34321 gccgacacag attcaggtag tcaggagaca tcagaagtga ccttgatgac cctgcatgct
    34381 gccaaaggtc tcgaatttcc agttgtcttt ttgattggga tggaagaaaa tgtctttcca
    34441 cttagtcgtg cgactgaaga tccagatgaa ttagaagaag agcgccgtct agcctatgta
    34501 ggtatcacgc gtgcagagaa aattctctat ctgaccaatg ccaactcacg cttgcttttt
    34561 ggtcgtacca attataaccg tccgactcgt tttattaacg aaatcagttc agacttgctt
    34621 gagtatcaag gtctggctcg tcctgcaaat acaagcttta aggcatcata tagcagtggt
    34681 agtatttcct ttggtcaagg tatgagtttg gctcaggctc ttcaagaccg taaacgcggt
    34741 gctgccccaa aatcaatcca gtcaagcggt cttccatttg gtcaatttac agctggcgca
    34801 aaatcagcat ctagcgaggc aaattggtcc attggtgaca ttgctctcca caagaaatgg
    34861 ggagagggaa ctgttctgga agtttcaggt agcggtgcta ggcaggaatt gaaaatcaat
    34921 ttcccagaag taggtttgaa aaaactttta gccagtgtgg ctccaattga gaaaaaaatc
    34981 taattttcca tccttctcac gaataataaa gtgaggagga tttttatgta cagtatttca
    35041 ttccaagaag attcactatt accaagagaa aggctggcca aggaaggagt tgaagcgctt
    35101 agtaaccaag agttgctagc tattttactc aggacaggaa cacgtcaagc tagcgttttt
    35161 gaaattgccc aaaaagtctt gaacaatctt tcaagcctaa cggatttgaa aaaaatgacc
    35221 ctgcaggaat tgcagagttt gtctggtatt gggcgtgtta aggccataga attacaagct
    35281 atgattgaac tggggcatcg tattcacaaa cacgagactc ttgaaatgga aagtattctc
    35341 agcagtcaaa agttggccaa gaagatgcag caggaattag gggataaaaa acaagagcac
    35401 ctggtggcac tctatctcaa tactcaaaat caaatcatcc atcagcagac catttttatc
    35461 ggttctgtaa ctcgtagtat cgctgaaccg cgagagattc tgcactatgc aatcaagcat
    35521 atggcgactt ctcttatctt ggtccacaat catccttcag gagcggtagc gcctagccaa
    35581 aatgatgatc atgtcactaa acttgttaaa gaagcctgcg aattgatggg gattgttctc
    35641 ttggaccatt tgattgtctc tcattctaat tactttagtt atcgtgaaaa gacagattta
    35701 atctaaagtt cattaacgac atagtcaaag agttttttat ctttgggacg attttcaaaa
    35761 agaagttctg gatgccattg gacaccgaga aaggcgacat catccgtact catgacagcc
    35821 tcaatgatac catctttagg atcatgagcc acaactttta aatttggtgc taagtccttg
    35881 atgctctggt ggtggaagga gttgatatga gagatttctc catagatttc ttggagaacg
    35941 gtatctggtt ctgttaccaa gcgttgagtt gtgtactcaa cagaagaatc ctgccaatgg
    36001 tcttcgatat cttggtacaa agttccaccc atggcaacgt taaagagttg ggtaccacgg
    36061 cagacagaga aaatgggctt tttctgttta atagcttcct tgatgagggt cagttcgaag
    36121 atatctcttt gaaggtgata gtcatcgcta tcaatggttt tgggttcgcc ataaaatttt
    36181 ggatcgacat tttgcccacc tgtcaagatg agcttgtcaa tcaaactgat atagtggcag
    36241 gccatttctt gatcaccaat cggtaggatg atgggaatcc ctccagaatc tttaacgcct
    36301 tcaacaaagc cttttgctgc gtagctcatc atgatgtcat catctggatg agctttttcg
    36361 tttcctgtaa tcccaataac tggttttttc ataaaatgat tttcgctttc taatcctctt
    36421 ttcgcatgaa gtagaggagg gtttggagtt cacttgtcaa atcgacatac tgaacgacca
    36481 cgtcttttgg taaatgcaga tggactggtg aaaaactgag aattcctttc acaccagcat
    36541 caaccaagag attagcaacc tcttgtgact tgacgctggg aacagttagg atagcagtct
    36601 tcacatcagc atccttgatt ttatccttga tctgagaaat cccgtaaatg ggaatcccgt
    36661 caggagtttg ggtaccgact tcaggatggt cgtctaggtc aaaggccatg ataatcttca
    36721 tcttgttacg ttcgtggaag cggtagtgga gaagggcatg gcccatattt ccaataccaa
    36781 ccagcatgac attggtaata gagttgtcat tgagcaaatc ggcaaaaaat gtcattagtt
    36841 ttttgacatc atagccaaaa ccacgacgac caagttcacc aaaataggaa aaatcacgac
    36901 gtacggtcgc tgaatcaata ccgatagcct ctgcaatttg cttagagttg gcacgttcaa
    36961 tcttttctgc atgaaatctc ttaaaaattc gatagtagag agagagtctt tttgctgtag
    37021 cttttggaat agcaaactgt ttatctttca caaaatcaca acctttctat tcttctattt
    37081 tatagaaaca ttgtgaaaaa atcaacaaaa ataagaaaaa actaagaaaa atcttagttt
    37141 tgatgtaaaa aatctgcatg agatagaaaa cggtagaggt ctccgaccag accctgataa
    37201 actttttggc ccctaaaagt cagagaagtc acataaagtg tatctggtaa ggttacacat
    37261 cctgacaaag tcaacatgag agcctcatga tcctcatact tgagagtacg ctctacatga
    37321 tagcagtcct tataggtcag ttcaaacatt ttggctctat ctttccgatt ttgtaaagac
    37381 accacgttct accaagctat ccatgaggaa gtagaatttt tcctgatgaa tatggtggtc
    37441 ttctgatttg aaaatatcaa ctagacgaag gccaaacttg tcagtgatat tgattttagc
    37501 ccctgtaagt tccttgttaa tgatgatttt gagttggaag ccttcaccgc tgtttggcac
    37561 tttttccaaa aggcgagtca gttcatagtt accaacctta gtttcaaaaa aggtgttatc
    37621 tttgagggtg aattttttaa cagaagggct aagagtgtaa tcgtaacgac aattttttaa
    37681 ctgaatgatt ttttcaaatg ccatatggct aacctccgat aatttctttt aaggtttttg
    37741 cgagggtttg taggtcttca acggtatttt gtggcgacaa actgatgcga agggattcct
    37801 tcaagcgttc tgaatttgcg ccatacatgg cttcaagaac atggctggat tggacaacgc
    37861 ctgcagtaca ggctgagcca gtagagattg aaattccagc taaatctagc cgaaggagta
    37921 agaggtcatt tttctgacca ggaaatccaa tattgagaac ataagggaga tgatgttttc
    37981 ctctattcaa gtaatactga atgccctcca gctctgccag aaaggcagtt tctagatttt
    38041 gtacatgttg aaaatgttct tcttgttttt ctaggtcttc ttttagggct gcaaccatgc
    38101 ctacgatagc aggcagattt tcagttcctg cacgtttttt ctgttcctgg tctccgccat
    38161 gtagatagga atcaaagtcc atgctagatg cgtagagaaa accgattccc ttaggaccat
    38221 ggaatttgtg ggcagaagca gtgagaaaat caatgcccaa ttcttctgaa tgaattggga
    38281 ttttaccaat agcctgaact gcatcaacat gataggcagc agggtgttgc ttgagtattt
    38341 ggccaatttc agcgatgggc agtaggtttc ctgtctcatt attggcaaac atggtagaaa
    38401 ccaaaatcgt atcgtcacgt aaagcctttt gaatttgctg ggctgtgatt tcttgatttt
    38461 ctggctggat aatggttgct tcaaacccaa agtgttgaac caagtaatca attgtttcaa
    38521 ggacagcatg gtgctcgatg gcagttgtga tgatatgttt tccttgttct tggtgacgaa
    38581 gacagtagcc aatgatggta gtattattgc cttcagtccc accagaagtg aaaaagatat
    38641 gttgaggttt tgtccttagt aactgggcta gttcctgacg ggcttctcgc aagagtttgc
    38701 cagcttgacg accatgacca tgaatactag aaggatttcc gtgggtttct tgcataacct
    38761 tggtcatagc tgaaatagca actgctgaca taggagtcgt tgcagcattg tccaaataaa
    38821 tcaaagaatc accttatttc tttttattgt aggcaaagag tgggctgact ggttttcttt
    38881 cgtgaatacg gacgatagca tcaccaatta actcactagc agtgatgtag catacatttt
    38941 taggagtttt ttcttttgtt gctactgaat cagtcacaag aatttcttta atattagtat
    39001 tgtcaagaag ctcagcagct ccctcgacga agagaccgtg gctagaaaca gcataaattt
    39061 ctgtagctcc ttcacgttca acgattttag aagcttcaga gaaggtacgt cctgtattta
    39121 aaatatcatc aatcaagata gctttcttac cttcaacatc accaataata taaccttcgt
    39181 tacgagttgc atcgtcttga gggtagtcga taatggcgat aggagcatca agatattcag
    39241 ccaggctacg cgcacgtttg acacctgaat ttttagggct aacgacaaca acatctgaac
    39301 caagcaatcc tttatcgcag taatgttttg cgaatagggg aacagtgaaa agattatcca
    39361 ctggaatatc aaagaaacct tgaacctgaa cggcatgcaa atcaagagtc aggatacgat
    39421 caactccagc cttaaccagc atattggcaa ctagttttgc tgtaagtggc tcacgaggac
    39481 aagcaatgcg gtcttgacgt gcatagccaa aatatggaag gacaacgttg atactgtggg
    39541 cacttgcacg cacacaagca tcgaccatga ttaacaattc cattaggtgg ttgttgacag
    39601 ggaaacttgt tgattggatg atgtaaacat cataaccacg gacactttct tcgatattta
    39661 cttggatttc tccgtctgaa aattgacgtg atgatagttt tccaagtggg gcaccaacag
    39721 cttgggcaat tttttgtgca atctcttggt tagagttgag tgcgaaaagt ttcatgtttt
    39781 ttctatctga cattatagac cgtcctctgt aaactttata aatcctagtt atatttacct
    39841 tacatatatg aactgggatt tgtgtatttt tatcttttct attttaccaa aaaatggaga
    39901 ttatttcagc tatttttcat acttttgaca aatcgaacca attttgaagg agctttttgg
    39961 taggaaatct gatttttctc taaaaattgt cggaaatcct gtttgccttg ctcatgattt
    40021 tccacttcaa gctccaattc gtaatctgtt atatcaaagt atcggctctg atccagtgcc
    40081 atgagaccaa tagctgtttt catttcatag cgaagcgttg ttagacaacc aagaacctgc
    40141 cagttcttac tttggatacc atgtttcgcc aattcatcca gtactagccc ttgaggaagt
    40201 tcttccttac tcagatagtt ctcagcatct tttagttgca atttttggtt gtattccatg
    40261 tttccaacac tctgcgggac tttgagtgtc aactcagccc agtcttcaaa ggttcgaatg
    40321 cgcatagcga ctttcttttc tcgcagttca aaatcaggcg tgtcgatgta gtaatttgtt
    40381 tgaagaacag gagtgacacc tgtgaactgg tcttttagac gattgtattc atcttttttc
    40441 aatagtgttt tcaattcaat ttctaaatgt ttcatttttc ttaccttttt ttatcgttga
    40501 aagcggattt atggtataat aagcattgta tttattgtat atgaatctgg agaaaaaatc
    40561 aaagatattt ttgacggata atatgagaac aagggagaat atatgacctt agaatgggaa
    40621 gaatttctag atccttacat tcaagctgtt ggtgagttaa agattaaact tcgtggtatt
    40681 cgtaagcaat atcgtaagca aaataagcat tctccaattg agtttgtgac cggtcgagtc
    40741 aagccaattg agagcatcaa agaaaaaatg gctcgtcgtg gcattactta tgcgaccttg
    40801 gaacacgatt tgcaggatat tgctggctta cgtgtgatgg ttcagtttgt agatgacgtc
    40861 aaggaagtag tggatatttt gcacaagcgt caggatatgc gaatcataca ggagcgagat
    40921 tacattactc atagaaaagc atcaggctat cgttcctatc atgtggtagt agaatatacg
    40981 gttgatacca tcaatggagc taagactatt ttggcagaaa ttcaaattcg tactttggcc
    41041 atgaatttct gggcaacgat agaacattct ctcaactaca agtaccaagg ggatttccca
    41101 gatgagatta agaagcgact ggaaattaca gctagaatcg cccatcagtt ggatgaagaa
    41161 atgggtgaaa ttcgtgatga tatccaagaa gcccaggcac tttttgatcc tttgagtaga
    41221 aaattaaatg acggtgtagg aaacagtgac gatacagatg aagaatacag gtaaacgaat
    41281 tgatctgata gccaatagaa aaccgcagag tcaaagggtt ttgtatgaat tgcgagatcg
    41341 tttgaagaga aatcagttta tactcaatga taccaatccg gatattgtca tttccattgg
    41401 cggggatggt atgctcttgt cggcctttca taagtacgaa aatcagcttg acaaggtccg
    41461 ctttatcggt cttcatactg gacatttggg cttctataca gattatcgtg attttgagtt
    41521 ggacaagcta gtgactaatt tgcagctaga tactggggca agggtttctt accctgttct
    41581 gaatgtgaag gtctttctcg aaaatggtga agttaagatt ttcagagcac tcaacgaagc
    41641 cagcatccgc aggtctgatc gaaccatggt ggcagatatt gtaataaatg gtgttccctt
    41701 tgaacgtttt cgtggagacg ggctaacagt ttcgacaccg actggtagta ctgcctataa
    41761 caagtctctt ggcggtgctg ttttacaccc taccattgaa gctttgcaat taacggagat
    41821 tgccagcctt aataatcgtg tctatcgaac actgggctct tccattattg tgcctaagaa
    41881 ggataagatt gaacttattc caacaagaaa cgattatcat actatttcgg ttgacaatag
    41941 cgtttattct ttccgtaata ttgagcgtat tgagtatcaa atcgaccatc ataagattca
    42001 ctttgtcgcg actcctagcc ataccagttt ttggaaccgt gttaaggatg cctttatcgg
    42061 tgaggtggat gaatgaggtt tgaatttatc gcagatgaac atgtcaaggt taagaccttt
    42121 ttaaaaaagc acgaggtttc taagggattg ctggccaaga ttaagtttcg aggtggagct
    42181 attctggtca ataatcaacc gcaaaatgca acgtatctat tggacgttgg agactacgtt
    42241 accattgaca ttcccgctga ggaaggcttt gaaaccttgg aggctattga gcatctatta
    42301 gatattctct atgaggatga tcactttcta gtcttgaata aaccctatgg agtggcttct
    42361 attcctagtg tcaatcactc taataccatt gccaatttta tcaagggtta ctatgtcaag
    42421 caaaattatg aaaatcagca ggttcacatt gttaccagac tagataggga cacttctggc
    42481 ttgatgctct ttgccaagca cggttatgcc catgcacgat tagacaagca gttgcagaag
    42541 aaatctatcg agaaacgcta ctttgctttg gttaagggag atggacattt ggagccagaa
    42601 ggggaaatta ttgctccgat tgcgcgtgat gaagattcca ttattaccag acgagtggct
    42661 aaaggcggaa agtatgccca tacttcatac aagattgtag cttcttatgg aaatattcac
    42721 ttggtctata ttcacctgca cactggtcga acccatcaaa tccgagtcca tttttctcat
    42781 atcggttttc ctttgctggg agatgatttg tatggtggta gtctggaaga tggtattcaa
    42841 cgtcaggctc tgcattgcca ttacctatcc ttttatcatc catttttaga gcaagacttg
    42901 cagttagaaa gtcccttgcc ggatgatttc agtaacctta ttacccagtt atcaactaat
    42961 actctataaa aactgtctca gagtataatt attatcttaa aggagaaaac tcatggaagt
    43021 ttttgaaagt ctcaaagcca accttgttgg taaaaatgct cgtatcgttc tccctgaagg
    43081 ggaagagcct cgtattcttc aagcaacaaa acgcttagta aaagaaacag aagtgattcc
    43141 tgttttgctt ggaaatcctg aaaaaattaa aatttatctt gaaattgaag gaatcatgga
    43201 tggttatgag gtcatcgacc ctcaacatta tcctcaattt gaagaaatgg tttctgcctt
    43261 ggtggagcgt cgcaagggca aaatgactga agaagatgta cgcaaggttt tggttgaaga
    43321 tgtcaactac tttggtgtga tgttggttta cttgggcttg gttgatggaa tggtgtcagg
    43381 agcgattcac tcaacagctt caacagttcg tccagctcta caaatcatca aaactcgtcc
    43441 aaatgtaact cgtacttcag gagccttcct catggttcgt ggtacggaac gttacctatt
    43501 tggagactgt gccattaaca tcaatccaga tgcagaagcc ttggctgaaa ttgccatcaa
    43561 ctcagcaatc acagctaaga tgtttggcat cgaacctaaa attgccatgt tgagctattc
    43621 tactaaaggt tcagggtttg gtgaaagcgt tgataaggtc gttgaagcaa ctaaaattgc
    43681 tcacgacttg cgtcctgacc ttgaaatcga tggtgagttg caatttgatg cagcctttgt
    43741 tcctgaaact gcagctctga aagctcctgg aagtacggta gctggtcaag caaatgtctt
    43801 catcttccca ggtatcgagg caggaaatat cggttacaag atggctgaac gcctaggtgg
    43861 ctttgcggct gtaggacctg ttttgcaagg tttaaacaag ccagttaatg atctttctcg
    43921 tggatgtaat gcagatgatg tttacaagtt gaccctcatc acagcagctc aagcagttca
    43981 tcaatagtga aaactataaa gcgatatact atgctatact gtagttatga aactatgtac
    44041 gaaaagcact gccattaatt cctgagaact aaattactga ttggtgtcaa aaaggaaaac
    44101 ttccaagcga taatatcctg tctatacacg acctatagaa atctgtaata tacatgtccg
    44161 taaaacgata aattcccttt ttgattttaa atgagtatga aaagagaatt ttttggctc
//
LOCUS       DAVKMU010000014        43286 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKMU010000014 DAVKMU010000000
VERSION     DAVKMU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.014"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..331)
                     /locus_tag="VW343_000837"
     CDS             complement(<1..331)
                     /locus_tag="VW343_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9755423.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKI"
     gene            complement(382..546)
                     /locus_tag="VW343_000838"
     CDS             complement(382..546)
                     /locus_tag="VW343_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9755424.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            complement(627..3278)
                     /locus_tag="VW343_000839"
     CDS             complement(627..3278)
                     /locus_tag="VW343_000839"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9755425.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVIAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(3271..3849)
                     /locus_tag="VW343_000840"
     CDS             complement(3271..3849)
                     /locus_tag="VW343_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9755426.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAEILQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(3849..4409)
                     /locus_tag="VW343_000841"
     CDS             complement(3849..4409)
                     /locus_tag="VW343_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755427.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHIFPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLDFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(4396..4844)
                     /locus_tag="VW343_000842"
                     /pseudo
     CDS             complement(4396..4844)
                     /locus_tag="VW343_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(5256..5651)
                     /locus_tag="VW343_000843"
     CDS             complement(5256..5651)
                     /locus_tag="VW343_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9755428.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQPAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(5747..6001)
                     /locus_tag="VW343_000844"
     CDS             complement(5747..6001)
                     /locus_tag="VW343_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9755429.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(6011..7345)
                     /locus_tag="VW343_000845"
     CDS             complement(6011..7345)
                     /locus_tag="VW343_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9755430.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYEYMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(7345..7578)
                     /locus_tag="VW343_000846"
     CDS             complement(7345..7578)
                     /locus_tag="VW343_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9755431.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            7609..7686
                     /locus_tag="VW343_000847"
                     /pseudo
     CDS             7609..7686
                     /locus_tag="VW343_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8027..8209)
                     /locus_tag="VW343_000848"
     CDS             complement(8027..8209)
                     /locus_tag="VW343_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755432.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(8211..8990)
                     /locus_tag="VW343_000849"
     CDS             complement(8211..8990)
                     /locus_tag="VW343_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755433.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            complement(9270..9620)
                     /gene="rbfA"
                     /locus_tag="VW343_000850"
     CDS             complement(9270..9620)
                     /gene="rbfA"
                     /locus_tag="VW343_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9755434.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(9871..12663)
                     /gene="infB"
                     /locus_tag="VW343_000851"
     CDS             complement(9871..12663)
                     /gene="infB"
                     /locus_tag="VW343_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9755435.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(12680..12979)
                     /locus_tag="VW343_000852"
     CDS             complement(12680..12979)
                     /locus_tag="VW343_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9755436.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(12972..13265)
                     /locus_tag="VW343_000853"
     CDS             complement(12972..13265)
                     /locus_tag="VW343_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9755437.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(13287..14423)
                     /gene="nusA"
                     /locus_tag="VW343_000854"
     CDS             complement(13287..14423)
                     /gene="nusA"
                     /locus_tag="VW343_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9755438.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(14467..14946)
                     /gene="rimP"
                     /locus_tag="VW343_000855"
     CDS             complement(14467..14946)
                     /gene="rimP"
                     /locus_tag="VW343_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9755439.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMGKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(15072..15707)
                     /gene="trmB"
                     /locus_tag="VW343_000856"
     CDS             complement(15072..15707)
                     /gene="trmB"
                     /locus_tag="VW343_000856"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9755440.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQDPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(15704..16498)
                     /locus_tag="VW343_000857"
     CDS             complement(15704..16498)
                     /locus_tag="VW343_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9755441.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(16659..17270)
                     /locus_tag="VW343_000858"
     CDS             complement(16659..17270)
                     /locus_tag="VW343_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9755442.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            complement(17421..17654)
                     /locus_tag="VW343_000859"
     CDS             complement(17421..17654)
                     /locus_tag="VW343_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755443.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            complement(17696..18385)
                     /locus_tag="VW343_000860"
     CDS             complement(17696..18385)
                     /locus_tag="VW343_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9755444.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            complement(18437..18820)
                     /locus_tag="VW343_000861"
     CDS             complement(18437..18820)
                     /locus_tag="VW343_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9755445.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(19449..19820)
                     /locus_tag="VW343_000862"
     CDS             complement(19449..19820)
                     /locus_tag="VW343_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755446.1"
                     /translation="MIKKNHILCEIVPGAVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            complement(20289..20408)
                     /locus_tag="VW343_000863"
     CDS             complement(20289..20408)
                     /locus_tag="VW343_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9755447.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(20512..20661)
                     /gene="blpO"
                     /locus_tag="VW343_000864"
     CDS             complement(20512..20661)
                     /gene="blpO"
                     /locus_tag="VW343_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9755448.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(20905..21108)
                     /gene="blpN"
                     /locus_tag="VW343_000865"
     CDS             complement(20905..21108)
                     /gene="blpN"
                     /locus_tag="VW343_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU9755449.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(21124..21378)
                     /gene="blpM"
                     /locus_tag="VW343_000866"
     CDS             complement(21124..21378)
                     /gene="blpM"
                     /locus_tag="VW343_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU9755450.1"
                     /translation="MNTKMMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            21658..23783
                     /gene="blpA"
                     /locus_tag="VW343_000867"
                     /pseudo
     CDS             21658..23783
                     /gene="blpA"
                     /locus_tag="VW343_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
     gene            23794..25155
                     /locus_tag="VW343_000868"
     CDS             23794..25155
                     /locus_tag="VW343_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9755451.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVISSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGIFKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            25212..25367
                     /gene="blpC"
                     /locus_tag="VW343_000869"
     CDS             25212..25367
                     /gene="blpC"
                     /locus_tag="VW343_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9755452.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(25411..26751)
                     /locus_tag="VW343_000870"
     CDS             complement(25411..26751)
                     /locus_tag="VW343_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9755453.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKFLL
                     GIIFTTFQFLAVSKYLSYFIEPLFGIGLSFLLLRGLPKKILIFYGLFPMILVELFYRG
                     VSYFVLPFLGQGIVDGDGNPIFLLIMIFVCFIVLVFLKWLDYDFTRLRREFLDTGFQK
                     SLTKINWAMGAYYLVMQSLSYLEYEQGIQSTTVRHLILVFYLLFFMGGIKKLDTYLKE
                     KLQEELNQEQTLRYRDMERYSRHIEELYKEIRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLRDSSQKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(26765..27502)
                     /locus_tag="VW343_000871"
     CDS             complement(26765..27502)
                     /locus_tag="VW343_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9755454.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(27507..27845)
                     /locus_tag="VW343_000872"
     CDS             complement(27507..27845)
                     /locus_tag="VW343_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9755455.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            28121..28450
                     /locus_tag="VW343_000873"
     CDS             28121..28450
                     /locus_tag="VW343_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9755456.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(28618..29667)
                     /locus_tag="VW343_000874"
     CDS             complement(28618..29667)
                     /locus_tag="VW343_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755457.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(29664..30395)
                     /locus_tag="VW343_000875"
     CDS             complement(29664..30395)
                     /locus_tag="VW343_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755458.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            30463..30873
                     /locus_tag="VW343_000876"
     CDS             30463..30873
                     /locus_tag="VW343_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9755459.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            30883..31170
                     /locus_tag="VW343_000877"
     CDS             30883..31170
                     /locus_tag="VW343_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755460.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            31332..31571
                     /locus_tag="VW343_000878"
     CDS             31332..31571
                     /locus_tag="VW343_000878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755461.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(31893..33029)
                     /gene="dnaJ"
                     /locus_tag="VW343_000879"
     CDS             complement(31893..33029)
                     /gene="dnaJ"
                     /locus_tag="VW343_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9755462.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(33234..33488)
                     /locus_tag="VW343_000880"
     CDS             complement(33234..33488)
                     /locus_tag="VW343_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755463.1"
                     /translation="MLVTVERNGNVIYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHIC
                     QELHLYKTQDPHYNKFEFDLAKRSLKITTRDNTTIFISNE"
     gene            complement(33601..35424)
                     /gene="dnaK"
                     /locus_tag="VW343_000881"
     CDS             complement(33601..35424)
                     /gene="dnaK"
                     /locus_tag="VW343_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9755464.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(35904..36428)
                     /gene="grpE"
                     /locus_tag="VW343_000882"
     CDS             complement(35904..36428)
                     /gene="grpE"
                     /locus_tag="VW343_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9755465.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(36455..37489)
                     /gene="hrcA"
                     /locus_tag="VW343_000883"
     CDS             complement(36455..37489)
                     /gene="hrcA"
                     /locus_tag="VW343_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9755466.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(37653..38090)
                     /locus_tag="VW343_000884"
     CDS             complement(37653..38090)
                     /locus_tag="VW343_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9755467.1"
                     /translation="MHLKGWRYRTSRFGLFYFDQCQPDDVIYRIYDSRFLKKYKHELQ
                     DFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMSKGLKWEVMRSRLRSCTTTFLGGLV
                     VCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFRLKRKYRVDE"
     gene            38770..41103
                     /locus_tag="VW343_000885"
     CDS             38770..41103
                     /locus_tag="VW343_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9755468.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            41116..42579
                     /locus_tag="VW343_000886"
     CDS             41116..42579
                     /locus_tag="VW343_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9755469.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFKDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            42579..>43286
                     /locus_tag="VW343_000887"
     CDS             42579..>43286
                     /locus_tag="VW343_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9755470.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDY
                     NFNLLLIALPPLSEQQRI"
BASE COUNT        13225 a         9249 c         7898 g        12914 t
ORIGIN      
        1 aaattttttt aaaattatag tgatagaaaa ataaatcaaa agcagaaaca tattctttac
       61 aatcacaaaa gacaacattt ttttcaatgc catcccatct gtctgtcgaa gaacttccaa
      121 tatatttatt tttgggtaat ctttccatct catattgttt ttgaggagca tatggttccc
      181 aataatctaa tccttttttt gttccagaac ggcctttaag aacttctaca tttctagaag
      241 ctttaacgtt ataatatgaa tagattaaac attgtttccc atccacttca tctatttgat
      301 caacatttgt actaaaccaa tattcagaca cacttttatt ggctggagaa ccatataaag
      361 cttgtaaaat tgaagtttta tttactccat atctattaca gacacctcag gattatttaa
      421 cttataagtt ttaacagcta cggaatcaat ttcaacagca acttgaacat ctatgcctga
      481 ttttttaagg ccacttgtag tgccacctgc accgttaaat aaatcaatag caacaatttt
      541 ccccatagta ttctcctaaa gtttctcctt tttattataa cattatcaaa tgtaaaaccc
      601 aacccgatag ggttaggttt ttaacatcat ttcaccaact tcttcatctc atcaatacgt
      661 gctacggtcg catcatactt ggcttggtag tcggcttgtt tgtctttttc tttttggaca
      721 acttctggtt tagcgttggc gacgaagcgt tcgttgctga gttttttgcc aaccatgtcg
      781 agttctttct gccatttggc aagttctttt tccagacgtg ccaattcttc ttcgacattc
      841 aagaggtcta ccagtggcaa gaagatttcg gctcctgtaa taacgcttga catagccagt
      901 tcaggtgcag ggatggttga tgcgatttcc aagtgttctg gatttgtaaa gcgtttgata
      961 tagttgacat tgctgttaaa gaaggcttcc aagtcgctat cgcttgtctt aacaaggatg
     1021 gtgataggtt tgcttggtgc tacatttact tccgcacgcg cattacgaac ggcacggatc
     1081 aagtctttga gactttccac accagtgtgg gcagcaaggt cctcaaaggc tgggttgaca
     1141 gttgggtatg ctgctgtcac gatagagcct tcagcgtact gaccaaagat ttcttctgtc
     1201 acgaacggca ttattgagtg aaggagacga aggattttat ccagcgtata gaggagaaca
     1261 gatcgagtaa ttaccttatc gtcttcattg tcgctgtaaa gaacttcctt ggtcaactca
     1321 acataccagt tggcaaattc ttcccaaatg aagttgtaaa ggatatgacc agccacacca
     1381 aactcgaact tatcaaagtt ttcagtaact tttgcaatgg tttcgttgag attgtggaga
     1441 atccagcggt ccgtcacatt accagcctca cctgttgcaa cttttgtgac attgtcatgc
     1501 gccacatcca gcgtcaaacc tccattgttc atgaggatat agcgagaaat gttccaaatc
     1561 ttgttaataa agttccatga agcatccatt ttctcataag agaaacgcac gtcttgtcct
     1621 ggcgcagaac cgtttgaaag gaaccatcga agggcatcgg caccgtattt ctcgataaca
     1681 tccattgggt caatcccgtt accgagagac ttagacatct tgcgtccttg ctcgtcacga
     1741 atgagaccgt ggataaggac gttttggaat ggttgacggc ctgtgaattc caatgactgg
     1801 aagatcatac gagacaccca gaagaagatg atgtcgtaac ctgttaccaa ggttgaagtt
     1861 gggaaataac gtttaaagtc ttctgagtcg acttcaggcc agcccatggt tgaaaatggc
     1921 cagagggcag aactgaacca agtatccaag acgtcttcgt cctgagtcca tccgtcacct
     1981 tctggagctt cttcgccgac atacatttca ccatcagcat tgtaccaggc agggatttgg
     2041 tgaccccacc aaagctgacg agagataacc cagtcgtgga cattttccat ccattgaagg
     2101 aaggtatcgt tgaaacgagg tgggtagaat tcgaccttgt cctctgtgtc ttggttggca
     2161 atggcgttct tagccaattg gtccatcttg acgaaccatt gagtagacaa gcgtggctca
     2221 actacgacac ctgtacgttc tgagtgacca acactgtgga cacgtttttc gattttgaca
     2281 agggcaccga tttcttccaa cttagcaatg actgccttac gagcttcaaa acgatccatg
     2341 cctgaaaatt caaaggcaag ctcattcata gttccgtcgt cgttcatgac gttgacttgt
     2401 ggcaagttat gacgttggcc aaccaagaag tcatttggat cgtgggcagg tgtgattttc
     2461 acgacaccag taccaaactc aggatctgcg tgctcatctc caacgattgg gatgagttta
     2521 ttagcgattg gaaggatgac gtttttacca atcaagtcct tgtagcgcgg gtcttctgga
     2581 ttaaccgcaa ccgcaacgtc cccaaacata gtctcaggac gagttgtagc aacttcaagg
     2641 gcgcgtgaac catcttccag catgtaattc atgtggtaga aggcaccttc cacatccttg
     2701 tgaatcacct caatatcaga aagggctgtg cgagctgctg ggtcccagtt gatgataaac
     2761 tcaccacggt agatccagcc tttcttgtaa aggtccacaa agaccttacg aacagctttt
     2821 gacaaacctt cgtcaagagt gaaacgctca cgagaatagt ctacagagag ccccatcttg
     2881 ccccattgtt ccttgatggt agtggcatat tcgtctttcc attcccagac tttcgtcaag
     2941 aaagactcac gaccaaggtc atagcgggaa atgccctcac cacgcaagcg ctcctcaacc
     3001 ttagcctgag tcgcaatacc agcgtggtcc attcctggaa gccaaagcgt atcaaaacct
     3061 tgcatgcgtt tttgacggat gataatatct tgcaaagttg tatcccaagc gtgaccaagg
     3121 tgaagtttac ctgtaacgtt tggtggtgga ataacgattg aataaggctt agccttttga
     3181 tcgcctgaag gcttgaaaac atcagcatca agccattttt ggtaacgacc agcctcaacc
     3241 tcggctggat tgtatttaga tgaaagttct ttagacatgt gtgtgtcctt tctctatttt
     3301 gtttatttta ttttgaattt gcttagcagc ttcttctgca gacaaattcg tattatttat
     3361 tttaaagtag tggtgcaact cattcggttg atattgggaa tttaattgaa gtatttcagc
     3421 ggtctctata atttctcttt cagatacctc aatatgtcgt tttaagggtt tgtactttaa
     3481 tcgattctcc gttcgatttc gacgtatgca ctcttcaaga cttgtttcca actcaacaaa
     3541 aagaatctct tgatgataat catccaacaa actctgaatt tgctttaaat atatcagatg
     3601 atactgattt gaaaaatcaa ttacatctgt taaaattact gatcgctgat ttcttgcact
     3661 cgctccaagg aatgaaaaag tgattccacg aacaaattcc cacatttcct ctgtatagtc
     3721 ctgatagatt tctagtgcaa aatcgatggc ttgatggtta taaaataggg tagcatccgt
     3781 cagtcgagat aattcttgac caatggtcat ttttcctgat gctggagcac caatgatgaa
     3841 aagatgcatc aaatcacctc ccattcactc ttcagcaagg catatcttaa atcatcacag
     3901 cagttgcctt gagcatcttt gcggtctctt atgcgagctt cgagggtaaa gtcaagcttt
     3961 tccgcgactc gtttactttg aaggttatat ccaaagcaag ttagttcaat cttgtgaaga
     4021 cccaaatctt taaaggctaa gtcaatcaag gcacgcgcag cttctggcac ataacctcgc
     4081 ccccaatagt ctgggtgtaa ggtatagccg atttccagca catcatcttc atggcgatgg
     4141 ttgaaatcga cagagccaac gatcttatcc gttcctttga cgacaatccc gtagcctgct
     4201 gggagatttt ccttttgatt acgctccgga aaaatgtgct ccagataata aatctcatct
     4261 tccaaggtct tgactggagg aaaacctgct ggataggcga cctctggcaa actagcatag
     4321 tcaaagatat cctcagcatc cgccacagta cggactcgta aaacgagacg ttctgtttcg
     4381 attttatctg gcagctcagt tcttgccatc cttcttcctc gcttttttga tgaaactgcc
     4441 cttcatatct acacgcttgt ccagatagca ataaacgcgc tgatatccat ctcccatgaa
     4501 ataggttggg caaacagttg atttttaaaa tgtccctttt catccaggag ttctggggca
     4561 acaagtcgct caagaatctt ggcaaagatg tggcaaatac cgtattcctc aacaatccta
     4621 tctacccgat aatctaaaac aagtggacag gcgtctaaaa taggagtctg agttcgttca
     4681 gaaatttcat aatgcactcc caaacgttcc aatttctcct gatgactgat aaaaccagcc
     4741 tgctccatcg caagcataga agtttcatca gaaatattca cagtaaattt ttgatattgt
     4801 ttgatctgct ctgcggcatt ctctctcgca acgactccaa tcacaaccca atctcctaga
     4861 ctataagagg aactacaggt catgatgtta tagctaaaat tctgatcttg atatcctaaa
     4921 ataaaaacag gaaaaccata atatagttta ttagttttaa atgattgttt tataacaacc
     4981 tcctttgact aagacacaaa agaaaagccc tgccatctac atgacaggga cgaatgtgtt
     5041 tatccgcggt accacccaat ttcgggccaa gcccgcatct ttagtcttta aaataaaaag
     5101 acagaaatat ttcatttttt cagtcattag caaccagttt tgacacattt gtggacttct
     5161 cagcgccgcc actttctgta aaatgtggga ttcaaaacac ctctaatggc tctattgtag
     5221 caagtttttc tcggaaatgc aaggctcaag aaaaattact tgaacttgat gccattttct
     5281 ttcaatttct tgatggtagc tgggccgatt cctttcaagg caaggagttc tttttcagtc
     5341 cagtttttga aatctgaagc agacttgatt ccttcatcat agaaagtctt ggcacgatca
     5401 agtggaagtc cccccaagtt tgctgcaaaa tcttctacag aattagctac tttttgagct
     5461 tgctctttgg tagcttctac tgcttgttca acttttttag aaacagcctt accagctgcg
     5521 cttgctggct gttctgcacg cttcacgact ttttttgtct catctacaac cttagtcaca
     5581 gtacttgaaa aggcacctgc gcgacggagg ccatttcgta attgtttttt acgattgagt
     5641 ttctttgaca tgataaaatc ctttcaataa aaaatccctg ctctgacaag gatttaacat
     5701 aaacattcta tcaagatttt agtgaaaaat caagaatctt tctcgtttaa taatttcgct
     5761 caccaaacaa gccttctggc aaatcatgga atcccttgcc tgctttgaag ttttcaaact
     5821 taccaagcaa gaactgataa gcatccaagc gactctcgta gcgaatcatg gcatctttgg
     5881 cacgctcgtc ctggtcttct tcgtaaactt tttgactttc cttgtgcgcc atgtcgttaa
     5941 tcatgatttg ggtattgacc caagcttcaa attccttcat aaattcttgt tcgtaactca
     6001 ttttttctcc ttattctaat ccacgaaaat agtccgtatc aatctcacgg tagggctgga
     6061 tatcctgaac atactccaac actccttgga attctccgtc ttcatcgtgt actgcagcat
     6121 aggtgatgtg gacaaactta cctcgcgact cagacttgaa ccacatttca tacttgtctt
     6181 tgcttccctc acgaagcccc ttcatgatag ttttgacctt gtccaagtac ttaggcggat
     6241 ggcagagttc gacattgcgc ccgacttgag acggcgtccg tttgaaaatc atctcatcag
     6301 ctggcgtatt gtcattgtaa tactggaaaa tatcatcttt attgacaaag gtaatctcca
     6361 tagggaggtg attgaggatg agattggcct gctcgactga aagatagcca ttaccaaaag
     6421 cctgttgact atggcggtcc agcacagctt ccttttcctt aggggtaaag gtaatggtaa
     6481 aatggccttc tggcgtatct atgacttgtt gaacttgacc ttctgccgta tctagctgta
     6541 caggctcctc tgcaatcttt tcctcaataa agctctgtcg ttctggcacc catttctctg
     6601 acggacggat gatggcatag ccataggcat cgctctcctc cgcaatctga agccagtcat
     6661 cctgagtaaa agactcaagg agaatcatga ggaggatgga ctcttccttg aaaatcatac
     6721 tttcaaactc tgtcgcaaaa gcttcaaaag cttcctttac actgctaatt gacacttctg
     6781 gtagtgactt ggctgtcgtt agagctgttt gaaagagttc cctaatctga tcatccactc
     6841 cccacataac tttgggaggt gaatcgtgtc catagcgctc catgatagga aagaagagtt
     6901 cttccttacg ttggtaatgg atgtcaaatt gacccacaag tcccatctga cgcaccaaac
     6961 ccttacgcat ctccgccagc atttcctcgt cttccatata ctcataggta tctaacaatc
     7021 tacgaatgcg aatcaaggcc gcacggagag ccagattttc ttctttgaag acacgaactg
     7081 ggtgacctgg atgctcagta tctgaaactt cgacaccttt gatagcattt ttaaaaagat
     7141 tggcatggac atcacagagt tccataacat cttcaaaggt gacacctgag tctgagttca
     7201 tcagctcgtg ctccataagg gaaatctcga tggctgagac acccgcaaag gtcgcatcaa
     7261 agcgctcttg aaccgactca ggagaggcgc cattgtgcaa ttctaacaaa atatcccgta
     7321 ggacatgaat ccgttcatct gccattagtc taatccaatc acttcgtagc cattcgcttc
     7381 cagtgtgcgt acaatcttgt ccataggagt tcctgctagc ttagaaccct gtttgagcga
     7441 taccttgcga ccaactgtat tgcgcattaa gggattggca aggggtttaa aacccaactc
     7501 cactagaatt tccaagactt ctggatgctt gtccaccact tctgcaacag gaattgacac
     7561 atcgatgata ttgtccatga cgacctccat tgtatgttct aatactcaat gaaaatcaaa
     7621 gagcaaacta ggaagctagc tgcagactgc tcaaaacagt gctttgaggt tgtggataga
     7681 actgacgaag tcagctcaaa acaccgtttt gaggttgtag atagaactga cgaagtcagt
     7741 aaccatacct acagcaaggt gaagctgacg tggtttgaag agattttcga agagtataaa
     7801 atgattttac tgcttatatt ataccatatg gagagagatt aaaaaactag cagtggggtt
     7861 cctgctagtt ttctgcgact tggattacca tctagataag tttaaagaac caatccaata
     7921 tcttgtaaaa cagcaattga actgcaaaag aaatgatcat ccaaaagaat ttcacaatcc
     7981 caataatcgg tttgataaaa agaatagcaa gaagaccaaa aaaatttcat tctctctcct
     8041 ctggcttaat gagccaccga gccgtatcca ttagcttatc tgcaataaaa agtttgccca
     8101 gaccaacaac gacatggtca gctttcttta tcgttttcat cacttttaca ccttgcacgg
     8161 tcaaaaagta attaccataa attttagcta gagtttttat gagtcccata ttactctcct
     8221 tcgatgattt cagcctcttt tcggattgtt tcaacatctt cttgatattg aacttcacta
     8281 tagttaacta gcttggacaa ttctttctgc aagctttcac ccatcggttt catagttgca
     8341 aaggttaaac cacctgaaat gataccaccc aagataggaa taaatttccc cattcctttg
     8401 gccagccctc ccttggtaag attcacacca aatattctta agactttttt caaaatagga
     8461 taccaaagcg tcttagttaa agctttatta ggtactgttt tcattacctg tttggcaatt
     8521 gttataccac cagcacgtag caaagcagca gttccattca cccccaacat gacgcctaga
     8581 taaagcaaga gggtattttg agcatcttca ctcaactcat cgcgtgaagc ccaaagatcc
     8641 tcataaccat aaatataacc taattcttgg gccaatttca gagagaaagc ataaaattga
     8701 gccacatcag ctggaatggt aattgccata gcaagccctc caggtaatcc tgccaaaaca
     8761 gaagtcccac tcgctaataa aacattatcc ctaatacagg cattagccac tcgatctaag
     8821 atttcttgag ataagagaga cgttggacct tgttccaata aggtaggaat gtcctgtgga
     8881 cccaattctt tggaaaactt atccactaaa aatttcgaac gatcaacctt aacaactggt
     8941 agttttacca cttgttgtaa tacttgcata gctaagtctt gttcagccat atacaaactc
     9001 cttatttctt ttttacacct tatcatatta tatttcctac aaaaaaagca ttttctaatt
     9061 agataacatt aaaaaataca gaaccccttt ttttatagta aaatgaaaca cgaacaggac
     9121 aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggcgtactat
     9181 tctagtttca atatatttta aaagaatatt tttccatttc aaattcaacc tattttcctc
     9241 caccaaaaaa gaggggcaac ccctcttctt tagttcttat ccagattgcg tagcatctcg
     9301 tcaatcttgt ttccatactc gatggactcg tctttgacga aggtcaaatc tgggattttg
     9361 tacaatttca aattgcgacc aagttcacgt ttgatggtac cagttgcttt ttcaagcccg
     9421 atttgggctt tttggttatc cgaagcaagg ttactcaaaa tggtgtaata aaccttggca
     9481 acagacaagt cacccagcat ctgaacatct gtgatggtca caccttggac acgtggatca
     9541 cggactttct tttgcaaaat ctcattgact tcacgcttga tttccatgcc cacacgatct
     9601 gtacggaaat gatttgccat gatattcctt tctctacttt gaaccaaaag ctaggccagc
     9661 agacctagct attttaaact tcttgatttt cagtttaaat tgaaacgaag ttcaatttga
     9721 agtcatctgc ttctttcgcc gtggtgaaag tgatggaact gatttatcag tcccatcaca
     9781 ggggattttt gagaccctag gctcaaaaat aagcaatgaa accatcggtt tgcttgcgtc
     9841 cctcaccacc taagaaagga gcaaaaaatc ttatctcttg atttcttcca tgacatacgc
     9901 ctcaatcaca tcatccatct taatatcatt gtagccgtcg atcatcaatc caccttcacg
     9961 gccgtttgta acttccttaa cgtcgtcttt atagtgtttc aagcttgcga gttcaccatc
    10021 atagataacg acaccatcac ggataacacg gactttagag tcacgggcaa ccttaccgtt
    10081 gataaccata aatccaccga tagttcccac tttagacacc ttgaaggttt cacggataac
    10141 tgcttcacca ataacttttt cttcaaattc tggatcaagc atccctttca tagcttcttc
    10201 catctcttcg ataaccttgt agataatgct gtgaagacgg atttccacat cgtcagcttc
    10261 tgcttgttga cgagcttgtg gtgtagggcg tacgttgaaa ccaacgataa aggcatttga
    10321 agcttcggca agggtcacgt ctgattcgtt gatagcaccg accgctgagt ggacgatagt
    10381 cactttgaca ccttccacgt caatcttttg aagtgaggca gaaagggctt caacagaacc
    10441 ttgtacatca gccttgatga taacattaac agatttgagt tccccagctt taagggtatc
    10501 aaagaggttt tcaaggctaa cacgttgggt agcttgacgt tgtttcatga gggcacgttt
    10561 ggcacgctct tcacctgctg cacgcgcaga tttttcatcc tcgtaaacgg caaagtggtc
    10621 acccgccatc ggtgcttcgt tcaaacctgt gatagagact ggtgttgatg gtccagcaac
    10681 tttaacacga cgaccaaggt cgttggtcat agcacggaca cgaccgaagg tatttccgac
    10741 aacgattggg tcttgaacat tcaaggtacc ttgttgtaca agaagggttg cgaccgcacc
    10801 ttttccttta tccaagcgcg cttcgataac cgtaccgatt gcacgcactg ttgggtctgc
    10861 cttgagttct tggatttcag ctacaagaag gactgtttcc aacaattctt cgatattttg
    10921 gttgaattta gctgagattt caacaaattc agaatctcca ccccaagcag ttgacataac
    10981 tccatgctct gccaattcac cgataacgcg ttctgggtta gcacctggtt tatcaatctt
    11041 gttaatggct acgatgattg gaacgttggc cgcttttgag tggttaatgg cttcgatagt
    11101 ctgaggcata accccgtcat ctgccgctac gaccaagatc gtaatatcgg taacagaagc
    11161 accacgcgca cgcattgatg taaaggccgc gtgtcctggt gtatcaagga aggtaatctt
    11221 cttaccattt tccacgattt ggtaggcacc gatatgctga gtaataccac ctgcttcacc
    11281 tgtcgcaaca cgtgagttac gaagagtatc caaaagggtt gttttaccgt ggtcaacgtg
    11341 tcccatgata gtaacaactg gtggacgctc aaccaattca tcttcattga gataaccatc
    11401 ttcgacaaag aaacgttcga tgtcagcatt atccacttca accttttgtt tggcttcgat
    11461 accgtaatcc accatgagga gttcaattgt ttccccatcc aaggattggt tttgtgtggc
    11521 catgacaccc atcatgaaaa gtttcttaac aatttcagct ggttcacgtt tgatacgttt
    11581 tgcgatttcc gcaacggtca taccatctgt atattcaaat tctgttggca attcatggaa
    11641 tttacgctcc gtaacaggtt ttggagtctg attacggttg ttcttgttat tgcctttttt
    11701 gttctttttg ttgttattcc agttactatt cttttgattt ctcacttgat tttgactact
    11761 tcgattcttt tgttgttttc taggaccatc ttcttcatga tcataatcgt cacgattttt
    11821 gtctggtcga gcttgttttt tacgacgtgt atccactgca ggttcttttt tctcagggac
    11881 gacttcaacc actgcttgaa cttgctgtgc ttgttcagct aacttagccg cttcttcaaa
    11941 gatttcctct ggttccttgc gtttgttagc ttgagccaag gcttctttag cagcctgata
    12001 ctgcttgaag cgttcctcac ttgaacgagc gtactctgca ttttgctctg cttttagggc
    12061 tgctgcacgg gctttaaagt caatacgtgg agccgcttga tttgaacgtt tgtcctcttg
    12121 ctgacggcgc tcatttctac gtttttgctg accttgctgc ttcttagctt ggtcattaaa
    12181 gcgacgattg tcgcggttgc tttgaccttg ttttccacca ttacggccat cgtttttctg
    12241 acggtttccg ttttgttgtt ggtcacggtt attgccctta ttttgcttgc gtcgttctgc
    12301 ctgctcttta gcacgggctt cacgctcagc cttgaaatta cggctttgcg gtttcgctgc
    12361 tactttttct gttttcggag caactggctc agccggtttt gcctcttcct tagctacagc
    12421 tggtttagct ggctcagatt tttcggtttt cttttctgca cttacttttg gtgctgcagg
    12481 ttttgcttct actttcggag cagctgcagg cttaaagctg gcagcaattt ttgcagcgac
    12541 agcttcttcc acacttgatg agtggctttt cacatccaag cccaactctt ttgcacgcgc
    12601 tacaacttct ttactttctt ttccaagttc ttttgcgatt tcgtacaatc ttttcttaga
    12661 caaatcatgt cctcctcttc tattccataa gagacctcat tttctttgta aatccagcat
    12721 ctgttacagc caaaaccttt ctcgatttcc cgactgctat gcttaattcc agtgttgaaa
    12781 acacggttac aatttctact tgataataat gacttttatc ttgaatcttc ttggtcagat
    12841 tgggtccagc atcatgagct agaaagacca acttggcctt gccgtcttga atggccttga
    12901 ccaccaattc ttcacccgat atgatgcgcc ctgctcgctg agcaagcccc aagagattac
    12961 ttatcttttg cttattcaag tcccaactct cttcttttca ctttgtgatc cacataagcg
    13021 atcaactcgt cataaaagct ttcttccact tccatgctaa agctgcggtt aaagaccttc
    13081 ttctttttcg cctctagggc ttctgcattg tctagtttga tataagcgcc gcggccattg
    13141 gccttgcccg taggatcaat aaagacttgt ccttccttgt tcttgacaat gcggagcaaa
    13201 tcacgcttat caatcacttc gttagacaca acagacttgc gcaaagggat ttttcttgtt
    13261 ttcatctttc cctcctctag cagcttttat tcttctacag tatcgttttc tacttccaac
    13321 tctactgaag cagcgtcttc catggcttca aattcgctag cagacttgat atcgatacgg
    13381 taaccagtca agtgagccgc caagcgcacg ttttgtccac gacgaccaat ggcaagagaa
    13441 agcttgttat ctggaacaac caccaaggca cgtttgctgt cgttttcatc aaagataact
    13501 tggtcaacct cagcaggagc gatggcattg tagataaatt cagctggatc tgctacccac
    13561 tcgataacat cgatattttc ttcgattggt accatgcggt catttttagc atcgtaacga
    13621 gctgggtgga atttgctagt aatcttcttg atattagcac caccacgtcc aacgattgta
    13681 ccgatagcat ccacgtttgg attgtggcta cgaacagcaa ccttcgtacg gtcacctgct
    13741 tcacgagcca cgctcatgat ttcaacagtt ccatcataaa cttctggaat ttcttgctcc
    13801 atcaaacgtt tgatcatttc tggatggcta cggctaacaa agacgttcac accacgaggg
    13861 ttgtcttcaa ccttgtaaac ataaacttcg atacgatcat gagaagcaaa aacttctcca
    13921 ggaatttggt cttgttttga caattgggct tcgatgctac caaggttgac atagataaag
    13981 cggttgtcaa agcgttctac tgtaccagac atgatttctt gctcatgttc tttgtaagta
    14041 ttgtaagtga tggcacgtgt ttgcttgcgc attttttcca tgatggtttg tttggcagat
    14101 tgggctgcta cacgaccaaa ctcagctggt gcttcttcaa acttgatttt gtctccaagt
    14161 tcataagctg aattaatggc aagagcatct ttcaagctga tttccaaacg gctatcaaat
    14221 acttcatcaa caacttcacg gacagtataa actgtaaagt cacctgtttt ttcgttgaag
    14281 tcaatagcta cgctgtctga ctgaccatag cgtctgcgat aagcggaacg aagcgactct
    14341 actactgcgt cgatgatatc ttcttttttg attcccttgt cttcttccaa aatgcggaag
    14401 gcctctagca tttctttact catgttttct tcacttttga atcctcaaaa gctatccttt
    14461 ctttttctat aatttaactg ctaaacgtgc ttttgatact aaactgtatg gaatttggac
    14521 ggttttctta cgcgtcttgc ccatatattc catagtcaac tcgtcctctt cgaaggccaa
    14581 caaggttcct tcaaagacct tttgcttatc gatggcttgg tagagcccga catggatgta
    14641 ttttccaacc gctccagcga cggcatcctt ggttttcaaa gggcgttcca aacctggact
    14701 ggtaatttct aggaaatatt gttctgggaa gggatctggc ttgatggtgt ctaggacagg
    14761 actgataatt tctgtcaagt ctgccgtgtc gttcaaggta attccttcgg gtttatctac
    14821 aaaaatactg agaatcatgt cactgccaat ctttccatac tcgatatcca cgagttcaaa
    14881 aggagcttct atgacaggtt ctacaacttc tctgactaat tctacgattg ttgcgattgc
    14941 gtccacctcc tcataagcaa gaggcgaaga tatttccccg cctcactttt catattctta
    15001 ctatcagtat agcacgtttg ccaacttatg taaagcaaaa gcacttgtac agcctaattt
    15061 taggctatct cttaaaattc tgcctcaact cggtagataa cttgcccctt gtttgagaat
    15121 ttttgctcgt attctgtcat gacattgcct tcaaaatcac tggcatgcaa atctaaccag
    15181 acaccattga gtttcatgcc atattgagaa aagctcacta aactgtactc aaacaagcca
    15241 cggttatccg tcttgaaatg aatttctcca ttttcaggca agatacgttt gaaggtatcc
    15301 aagaaggtct tgtaggtcaa acgacgcttt tcatggcgtt ttttcggcca tggatctgaa
    15361 aagttcagat acaagcgatc aatctcaccg tcttcaaagt agtcagttaa gtcagaacca
    15421 tctacccaca agagcttgat gttaggcact ccaacttcaa gcaccttgtc caaagcgtag
    15481 ctcaaaacag acttttgaat atcaatcccg atatagttga tgtcagggtc ttgcttggcc
    15541 atacctgaaa caaaggcacc ctttccactt ccaacttcca catgaatggg attatcattg
    15601 ccaaacaagt cccgccattt tgccttggct tccaagggat tgaggaccac atactgggga
    15661 tttgcctcta gtaattctgt tgccccttta cgatttctaa ctctcatctt ctctttccat
    15721 acttgtctcg gaaatgacgc aaaccatgaa tctcccgatt gacattttct aaatcttggt
    15781 tcatataata cttggaaatc tgactcaaat aagacaattg accataccaa tacaatttac
    15841 ttaataccgt ttgattgtac ttgtaaccgt agtaggtcaa ccattccttc cactgatgtt
    15901 ctgaaatata atggcagagc atatgggcca catcaaacat gcgatcggtc aagcgaaccg
    15961 aatcccaatc tactaaataa atcaagccac tatctgtctc aatccaatta ctatgtcgta
    16021 catctccatg gacaatggtc gcatggtcct ctctaaatcc tggaatagtc tgacgtaaat
    16081 cagccatcac ttcactgata aaatgatttt tacgcaaagc atctggagcc gtttcctgcc
    16141 aagactgtag taaatctaca ggtgtttcca tggcatagcc caaacgactc aattgtgtca
    16201 tcaacggacg tgagcgatga agacgggtta aaatattgac gatttgctta cgattcatat
    16261 catagggggt caatatcttg cctgtcaacc attcttgagc acacatatca cgcccatctg
    16321 ccaaacgacg actccataat aattgtggag caatttgttc tctagctaga ccaggtagga
    16381 ttggagaggt gttcattttt acaaagatgc gcttcccatc aggatagcta cccatataag
    16441 ccttgccact tttcccaggt atgggagtca gtgttagctc attatcaccc aaatccattt
    16501 ctttcctccg tataaagttt ccttactatt ctactagttt ttggcctatt cgtcaaccac
    16561 tctaacccga ttctcaaaga aaagcctctg gattggcttg ggtatcatta tagcttgaaa
    16621 aaggtaagca ccgtcttctg cttacctttt cataattttt ataaataata taagagtggc
    16681 caactgttgt aaagcatatg gactagagta gaaacccata ggttttgact ctttttataa
    16741 gcaaagccca gcaacaaacc cccaagtaga taaaaaataa acgaagggac attagaaggt
    16801 ccatgcatga aagaaaagag agaggaagtt agcacaagcc ctaaccaaga attgtcaaaa
    16861 accgcacctt gaagaagtcc tctgaaaatg agttcctcct ggatggggcc aaaaactgag
    16921 gtagtcaaaa tcaaagacag ggaaattcct ctgctgactt ccgccaagtg ttggacccct
    16981 gagccagaag aaacagcgaa gaaatgaata taaaccagcg tctcaagtac acttagaaga
    17041 aagattgcaa tgaaaagcaa aagttgtttc ttgcttaaca tcccaaaaga aatcatttca
    17101 aactttctag cataagcaac actcagcgag gttacaagaa gacttacaag aatcaatgca
    17161 gattcatttt gaaaaaaacc cccatggtta atcgtataag gaactgtaat gacaattatt
    17221 agtactaaaa atccaaaaca caaggcatga aatttatcaa aaaactccat aaaattatcc
    17281 tcctcaacaa acagacttcc tacacgtcat tctttagttc tagcctttcc aaaacaaacc
    17341 attttatact cttcgaaaat caaattcaaa ccacgtcaac gtcgccttgc cgtactcaag
    17401 tacagcctgc ggctagtttc ctagtttgct ctttgatttt cattgagtat tagtaaccaa
    17461 aatccgacca catagccagc ccctatgaat atagccatta aagctagcat ggaatttagg
    17521 aaattaaaaa ccaccgcaga tacaaaggtt agcacaaaaa cattaaaagc aatggtgtca
    17581 gaagccaaga ctagaatata gggtgtcaac cgatctaaag ttttggaatc taggaaaaat
    17641 aagtgtttat acatgatgac ctcctctatg gctgaaaagc aagccttttg tttttttacc
    17701 ccaagaccct atgtagaaaa gtgagcaaaa acgggaaggt cgctacaata ttattgatca
    17761 catgcaccgc ataggatgga taaatgctct tggtatagcg ggtcaaacca gcaaagatga
    17821 ttccaactgt tgcaaagacg aagatatcta acagactagg caggcttgaa aaatgaggga
    17881 gagcaaataa aatagaagga agaagcaaat caagaccaaa tcgcgaatgc ttaaagaaag
    17941 catgttgcag taatcctcta taaatcaatt cttccatcag tggaaccaga aagaacaggg
    18001 ctatataaat acctagctct gcaaagttag tcccactata accaatcaat acagcccaac
    18061 cttccgcagt tgactgaaca tgtttagctg tctgaacgtt aaaagagatc tggaacacta
    18121 ccactaatac tgtcaaaatc gaataccaaa gccatttttt tcttggaatg cggaagagat
    18181 aaccatggcc tgtcttaaca agaaccacaa tcatgactcc aataaaaagt aaactcaaga
    18241 tattttgaat ccagaataaa ttgcctatct gagaagaaaa ttgccaatag ttttggacga
    18301 taagcgtcag ctgagaaaga ccaaatacga aaaataagta agagaagact gcacttattt
    18361 tgaatagaag ttgatacttt ttcatagaaa tcctccctac tataacctca ccttgtcagg
    18421 ctctactgct gtaagattaa gaagacagtt tgtttttttt aaggctaacc tgactactag
    18481 ataatagata cattaaggca ttaaagacaa tgaaaatatg tccatagaat aaaatcaacc
    18541 tcgcatccaa accaagataa agtttgatta tcaaaaagat gagcaaaaga atttgaaacc
    18601 ataaggtttt tccaaaaata aatttaaagc gatttcgaat atctacttcc ttgattttta
    18661 ccgccacccc tttattagca agaaggaaaa ctcctgcttc aaacaaacca ctgtaaagaa
    18721 caagccaccc aatagatacg atagagattt gtaaaaatgt ccctaaaaga atatccaaca
    18781 cactactcaa gaaaataaca aaaaataatc tgtatttcat attaaatacc tccattcatt
    18841 tatttcacta acaatttaat agagccttct actcaaatat cctgtcagaa aaggatagaa
    18901 agctactttt tataatactt caagccccac atgagcagaa gcgtgataaa caagcagaga
    18961 atacacctat ataagcgatt agttgttgat agaattctgt ttctgaaata cctctataca
    19021 aacaaatgac aaacataaaa tctgccaagc cgataaacat aagttgattg gttctaggac
    19081 taaccaaatc atcatttact tatatttaag agtatctctt ttatgttagt gtatgttaac
    19141 actaaaaaac aagttaggcc aataatattt aaaatgaaca ataacggggt taagtctcta
    19201 aaaaaattat ctactgacac tacaagaaat actatacata ttatagtcga aactatcttt
    19261 ttcttatcca taattattta ctcctttcct aacaaatcca acttatcaat caagagcgat
    19321 ttttaacata atgtagcagc acccgttgca actttgacaa gtttagtata tcattgtttt
    19381 ttaaaatttt tcatccaaat cttgaattgt catcgaaacc tcctaaattg caaaaacttc
    19441 attagcccct actttttata atacttcaaa ccccaaatga gtagaagcat gatgatcaag
    19501 caaagaatag caccaatata agcgattagt tgttgataag attctcctgc tgtgatacct
    19561 ctatacaaac aaataataga cataaagcct gtcaagccga taaacatgag ttgattggtt
    19621 ctaggactaa ccaaatcatc gtcttcaaac tcttttatcc gcatttccct agtgaggtaa
    19681 acagtgacca aaatagaagc taagttaata accactaaaa gaaattggaa aactgacgaa
    19741 atagaaatag ataagtagaa acaagcaaag gcaactgccc caggaacaat ctcacaaaga
    19801 atatgattct ttttaatcaa gaaaatcttt catttctttt ctttcttttt aattaaagaa
    19861 aaatagatca taaccgcaat catataggct atggtataaa atagattata ccaaatactc
    19921 tccctaagcg gataaagaaa gatggacatg atcagataca gaacgaaaat aatcagtatt
    19981 tttttcttca taagatttcc tcctaaatgt gcgatttatc ttagttgagc aagaacattt
    20041 acactgctag tatagcactt attttgacct tggatcactc aaatcataaa tggtcatcaa
    20101 aacctcttga attgtaaaaa ttaaaaaaag caagcatgaa aaacatactt tcctctttat
    20161 gttgtattga taccaacttg tttgtagact tttcatcctg ctatcacata tcattttgac
    20221 aggcgaaaca atattaaaga aactcccctg taaattaagc tagcaaatac aggggagaaa
    20281 tttatttttt agagagtact atccgtatcc tttttggaag attttgaaaa tatttttcta
    20341 attaagtcat ccatataagg accaaatata ccaactacta aaccaataat aaaactttta
    20401 aaatccataa ttaccaccaa catgttgcta cataggctac acctccaagt atagctccac
    20461 ctgcagcacc agttacacct attcctatag caaatggtcc caatagaaat gtcaaaccgt
    20521 tgttgcacac ccatcaattg cgccatatgc aacccctgct gcacaactaa tttttcttcc
    20581 ccaatcaata tctccacctt caacgcaagc aagcatttca ttatccataa cagaaaattg
    20641 tgacatcatt tttgtattca tatagtatgt cacttttcag ttacggaaca agtttaatat
    20701 aaaaattatc aaaaaaacat aggcaataaa gagaaaaatt aatttatcat agattagaaa
    20761 taatatgaca aaacaattca atgatgttaa ttcaatagtc ttttgttttt tatcagagat
    20821 acttatggat agataaataa gataggtttg aaaagcgaag agaataataa agaatatagc
    20881 cttcataaaa tttagctttc atttttatga tgtagcggta taggctaaat atccacaaac
    20941 cactgctcct ccaattcctc ctattgcagc gccccatggt cctagaattt tcccatattt
    21001 cactccaccc gctgcacaac ctaaagcagc aactacagct gctcctccgg aattacctcc
    21061 ataaacctca ctcagcattg tttcatttat attacaataa gtattcatac aagtctcctt
    21121 ttattaaaat ccacccgttg cccctgttac tcctgcccaa agatccacac caaatttagc
    21181 tcctatgtat ccacatgctc ccataaatgg tgctccaaca ccactcgcag cacaaatagc
    21241 tgtccctagc ccccagccac caaaagcagc accaccacct tctaagacat tagtttgcca
    21301 attattcttg cctccttcaa tactagataa catagttata tccatttcat gaaattgttc
    21361 catcattttt gtattcatga caaatactct tttttatttt taatttttgt cttgttgtaa
    21421 ctttgacaag tttagtatat catcgttttt taaaattttt catccagatc ttgaatagtc
    21481 atcgaaacgt cttgaattag cttttcattt gaaaccacct ctaaattttt taaaaaaaga
    21541 taatttctaa ttatttttta ccattcagga agtttcaatg actattcaag atttaataaa
    21601 atatgaactt atttttatgg cataatatac ctatctacta tatgaaagga attgccaatg
    21661 acttcttata aacgtacatt tgttcctcaa atagatgcga gagactgcgg tgttgctgcc
    21721 ttagcctcga ttgctaaatt ctatggttca gatttttctc tagctcactt gagagaactt
    21781 gcaaagacca ataaagaagg gacgactgct cttggcattg taaaagccgc tgatgaaatg
    21841 ggctttgaaa caagacctgt tcaagcagat aaaacgctct ttgacatgag tgatgtcccc
    21901 tatccattta tcgttcacgt taacaaagaa ggaaaactcc aacattacta tgttgtctat
    21961 caaacaaaga aagactatct gattattggt gatcctgacc cttctgtaaa aatcactaaa
    22021 atgtcaaaag aacgcttttt ctctgaatgg actggagtag ctatttttct agctcccaaa
    22081 cccagctatc aaccccataa agataaaaag aatggtctac taagcttcct tcctctgatt
    22141 ttcaaacaaa aatctctcat tgcttacatt gttctctcaa gcttattggt cactattatc
    22201 aatatcggtg gttcttatta tctccaagga atcttagatg aatacatccc aaatcagatg
    22261 aaatcaactt taggaatcat ctcagttggt ctggttatca cctatatcct ccaacaagtc
    22321 atgagcttct ccagagatta agcattgatg tgattttatc ctatattcgc catatttttg
    22381 aacttcccat gtctttcttt gcgacacgtc gtacaggaga aatcatttca cggttcacag
    22441 atgctaactc tattatagat gccttggctt ctaccattct ttctcttttt ctggatgttt
    22501 ctattctgat tcttgtaggg ggcgtcttac tggcacaaaa ccctaatctc ttcctccttt
    22561 ctcttctttc cattcctata tacatgttca tcatcttttc ttttatgaaa cctttcgaaa
    22621 aaatgaacca tgatgtcatg caaagtaatt ctatggttag ctctgccatt atcgaagata
    22681 tcaacgggat tgaaactata aagtcgctca cgagtgaaga aaatcgctat caaaatatag
    22741 acagcgaatt tgtagattat ttggaaaaat cctttaagct cagtaaatat tctattttac
    22801 aaacgagttt aaagcaggga acaaaattag ttctgaatat ccttatccta tggtttggcg
    22861 ctcaattagt catgtcaagt aaaatttcta tcggtcagct gattaccttt aacacacttt
    22921 tttcttactt tacaactcct atggaaaata ttatcaacct ccaaaccaaa ctccaatctg
    22981 cgaaggtcgc taataaccgt ttgaacgaag tctatctagt cgaatctgaa tttcaagttc
    23041 aagaaaaccc tgttcattca cattttttga tgggcgatat tgaatttgat gacctttctt
    23101 ataagtatgg ttttggacga gataccttaa cagatattaa tctcacgatt aaacaaggag
    23161 ataaggttag cctagttgga gttagtggtt ctggtaaaac aactttagcc aaaatgattg
    23221 tcaatttctt tgaaccctac aaagggcata tttccatcaa tcatcaggat attaaaaaca
    23281 ttgataaaaa agtcttgcgc cgtcatatta attacctacc ccaacaagcc tatatcttta
    23341 atggctctat tttggaaaac ttaaccttgg gcggtaatca tatgattagt caagaagata
    23401 ttctaaaagc ttgtgaagta gctgaaatcc gtcaagacat tgaaagaatg cctatgggct
    23461 atcaaactca gctctctgat ggagctggtc tatcaggagg acagaagcaa cgaatcgctc
    23521 tcgctcgtgc tcttttaact aaatctcctg ttttaatact agatgaagct actagcggtc
    23581 ttgatgtctt gactgagaaa aaagttatag ataatcttat gtccctaact gataaaacca
    23641 ttctctttgt agcccatcgt ctcagtatag ccgaacgaac caaccgtgtc attgttcttg
    23701 accaggggaa aatcattgaa gttggtagcc accaagagtt aatgcaggcg caaggtttct
    23761 accatcatct attcaataaa taaggagaat gtcatgaatc ctaatctttt tagaagcgtc
    23821 gagttttatc agagacgtta ccataactat gcgacagtgt taattatacc tctttcatta
    23881 ctatttactt tcatcttgat tttctccctt gttgccacaa aagaaattac tgtcacttcc
    23941 caaggagaaa tcgcccctac aagtgtcatt tcatctattc agtcaaccag tgataatcct
    24001 atccttgcta atcatttagt ggcaaatcaa gtagttgaaa aaggggactt actcatcaaa
    24061 tactctgaaa caatggaaga aagtcagaaa actgccttag caactcaatt acaaagactt
    24121 gagaagcaaa aagaaggact tggaattttc aaacaaagct tagaaaaagc gactgatctt
    24181 ttttctggcg aggatgaatt tggctaccat aataccttta tgaattttac taaacaatcc
    24241 catgatattg aactgggtat ctcaaagact aacactgaag tttcaaatca agctaatctt
    24301 accaatagca gttcatcagc catcgaacaa gaaattacaa aagttcaaca acaaattgga
    24361 gaatatcaag agctgagaga tgctatcgta aataaaagag ctcgcttacc aactggcaat
    24421 ccgcaccagt caattttaaa tcgttatctt atagcctctc aagaacaaac acaaggaacg
    24481 gcagaggagc catttttatc tcaaattaat caaagtattg caggtctgga atcatctatc
    24541 gcaagcctca aaattcagca agctggtatc ggaagtgtag caacttatga taatagttta
    24601 gcaaccaaaa ttgaagtact ccgcactcag tttttacaaa cagcctcaca gcaacaacta
    24661 accgtggaga atcaattaac agaattaaaa gtacaactag atcaagcgac acagcgctta
    24721 gaaaataata ccttaaccgc tccaagtaaa ggtatcgttc atctgaacag cgaatttgaa
    24781 ggtaaaaata gaattccaac tggtacagaa attgctcaaa tattccctgt catcacagat
    24841 acaagagaag tactaatcac ttactacgta tcttctgact atctacctct actagataaa
    24901 ggacaaactg taagattaaa actggagaag attggaaatc acggcaccac catcatcggc
    24961 caacttcaga caattgatca aactcctacc agaacagagc aaggaaatct ctttaaatta
    25021 accgctcttg caaaactatc taacgaggat agtaaactca tccaatatgg cttacaaggt
    25081 cgcgtcacta gtgtaactac aaagaaaaca tattttgatt atttcaaaga taaaatttta
    25141 acccattctg attaattttc agataacact ctataactat ttattatctt atcaaaaagg
    25201 agaatcataa catggataag aaacaaaacc taacttcatt tcaagaacta acaactaccg
    25261 aactcaacca aattataggt ggaggatggt gggaagattt cttatataga tttaatataa
    25321 ttgaacaaaa aaatacaaaa ggatttcatc agccaataca actataaaaa aacaagacca
    25381 agagaacgca ctctcggtct tattttttct tcattctata tgtattacag taagtacctg
    25441 acgaaaaacg tgatcttggc aggtggtatt gagactggta ttgggatgac tttccacaat
    25501 tttcataacg gtatagagac caactcctct ctcctcccct ttagaacttg ctccaaagga
    25561 gaagatttca gaaatatcga ttccctcttc tttgatggaa ttttcaatga taaaggtctc
    25621 ctgtgctcca tttttaaaaa aggcaattga aacatgaggt tgacaggctt ctacactagc
    25681 ttcaatagcg ttgtcacaaa ggatagacac aacggttaga aaatcaagta gactcatccc
    25741 ctctacctga atctcctctg gaacttcgac attaaagaca atgttcttat ctctagcttt
    25801 tataaatttt ccagctagga gacttttgag ggcacgatcc cgaacattca ccaatcggcc
    25861 aaggtcatat ttattgtcct gcaatttctg actggaatcc cttaagaccg agtcgtagat
    25921 ctcttttatc tgctccatat cctcctcttc aatgcccaaa cgtaagctgg ttaagaggtt
    25981 agtgtagtca tggcgaaaac tccgaatttc cttgtaaagt tcctctatat gccgactata
    26041 gcgttccata tctctgtagc gcaaggtctg ctcttggttc agttcctcct gaagtttttc
    26101 cttcaaatag gtatccaatt tcttgatacc ccccataaaa aagagtaggt aaaacactag
    26161 gatgagatgg cgaacagtcg ttgattgaat accttgttca tattcaaggt aagatagact
    26221 ttgcatcact agataataag cccccattgc ccagttaatc ttagtaagag acttttgaaa
    26281 acctgtatct agaaactccc ttctcaatct agtgaaatca tagtctaacc atttcaaaaa
    26341 gactaaaact atgaagcaaa cgaatatcat aatcaataaa aagataggat tgccatcccc
    26401 atctacaatt ccttgcccca aaaatggaag cacaaaatag gaaacacctc tgtaaaagag
    26461 ctctactaat atcattggga agagaccata aaaaataagg atttttttag gaagccctct
    26521 taacaataag aaagatagac ctataccgaa caaaggttct ataaaatagg acaaatattt
    26581 acttacagcc aaaaattgaa aagtcgtaaa aatgatccct agaagaaatt tcagaagaaa
    26641 ggccttaaaa atcctctcga aagtaagatc aattccatcc accttaaaga agatgacaat
    26701 ttctagtcca ttagtaacaa gtgtatacaa caatatccaa gcaatgttca taaattctcc
    26761 tagctcagtg taatttattg atggcctcag acacttccct gaccttataa cgggcgatta
    26821 gacaacttcc accattggga aagaaaagca gtttttcttt cttatccaaa tgcaccacat
    26881 ttgcaggatt gatgagaaaa gagcggtggc actgcaagag acggggctcc tgcttgaaaa
    26941 cctcctctaa actcgctgta aattccagcc tgtctgtctt ggtatagaga ataacacgat
    27001 gggctctggg cgacgtttcg agatagtaaa cctctttaaa aggatactga aattgggcaa
    27061 attttgattt aaagtaaaag caatcttccg ccagactttt actatcttga ctattggcat
    27121 agaggagggc tgtctcgatc cgagattcaa actcctctgc tgacaaggcc ttatcaatgt
    27181 agtccaaagc agacacttgg tagcgaaaag acaggggcat aaactccgag tgagtcgtca
    27241 caaagacaat cagggcataa ggatcccgat cccgaatctt tctagccact tccagtccct
    27301 tcatctcttc atttcgaatc tcaatatcca aaaagaatag ctgatgggcc cccttctcat
    27361 gcacttcagc cagcagttgg tccggcttgc caaatacctc aaaagagcta ggaatgatat
    27421 gatgtgcttt caaaagtttc tcaatcgtcg tttcaattct agtctgttgg gaaaaatcat
    27481 cctctaaaac aaatattctc atcttcttac tctccctctt tcaaccattt ttgacgaatt
    27541 tctctatagc gacgtctggc atacttgaca gcgtattgac cttcttctcc gagaaaaagg
    27601 attctttctt gaaaatcaat cgattttaat ttatcaagat taaccaaaca atttcgatga
    27661 catctcatca aggtttcccc acattggttc tcaagattac ttaaattttg aagcatatta
    27721 aaactagctt cttctgtaac aatctgtacg gtatgggctt tagttggatg tgtttggata
    27781 tagtagatat cgtcgatgtt tactttatag actgttttct gcgtctgaat aatcatatat
    27841 ttcattggat tctcctccta agaaaagaat aacagaaaag gtctagccca agcacaccca
    27901 atcctaaaca gtctttgaaa actcctaaaa ggattatttt ctacaacatc atgatgagct
    27961 tacaactatt ttatcataaa attcactcag cgcaattcaa gacatttcaa tgacaattaa
    28021 agatttgaat aaaaaaaaag aattaaaagt gataaactaa cagtatcaaa acacagttgc
    28081 cagaagtaag actggcaccc agattaaagg aggcattccc atgacagata cagaccccat
    28141 caaaagagct catactttga ttactgactt aaacaaagcc taccaagcat gcaaacaggc
    28201 aagcgctgac gacgtccgct ttcaggagca attaaactct attcttggtt ttctagccaa
    28261 ggctgaaaca gtggataatc gattcttgat tgaactagaa aaattttacc agacttccag
    28321 tcttctcatg ggactcagcg ctcttgatcc agatgctcca actcgcgccg cttggcgggc
    28381 ctatgaccgc ttccactttg accaagtcaa gaccaagtta atactcaatg aaaatcaaag
    28441 agcaaactag gaagctagcc gcaagctgta cttgagtacg gtaagacgac gctgacgtgg
    28501 tttgaagaga ttttcgaaga gtataagtct ctacggacca accattatcc tgtagtaaca
    28561 aaaaagaagt tgagacagac tgaactcaac ttctttttta gtgtcgtatc agcaatgtta
    28621 gtcctgcatc tgacgtttta cctgataagg caaatacaag actagtaaaa ccaaaccagc
    28681 tcctagtaag gctagaaggg ctagtttttc ttggaaggta atcaacccaa caactaattc
    28741 cacaagtaaa acaaaactgg tcaatcctcg aactacctcc tgtaagcctt tttccttttg
    28801 ccccttgtcc agcggaaaga gttgggtcaa atactggtag tcaaaggcat gatagagggc
    28861 cagcaactgg aagagcaaga ggtagttaaa gagaactacc actgctgtcg caatccaagc
    28921 ttgctcgata aaaacctgcg ccagtacgga aagtaagaga agacgaagac tgagagcaaa
    28981 gaggtcgcca tttcgcagat aagaacgcag atagagattt tgccaaatct tcccaggcac
    29041 cttctgaaca gcctttaaga taaagtccag ataggcacga cgcttaacgc tgtttgaaat
    29101 tcccttgacc tgcgtaaaga gggcaaagaa acgaagcaag acttgcttac gcttgctttc
    29161 ttgggaaatg acatagtccc agtccagccc agtttcagtg aaaaatttgc tggccttttg
    29221 acgaaagtgg aaatattttc ctacccccaa taaaagcaca tagagcagaa aaactggcaa
    29281 gccataaccc attgctaaaa ataaaggcgc aaataacagc aagaaaaggg tctgtacaaa
    29341 gagccaaaag actagggaaa tgccagtttg acgcttgaga tggagcttga tttcctcttc
    29401 tccaactaag agaaagagct tgtctggagc ctccatatag gtggcaattc ctccccaaag
    29461 taaaagtaaa acagacgtaa ttcctacaaa caaaaggata ggccaatgat tttcaggaaa
    29521 atgttgtaag agttgactgt actggtaggc tagaaagccc aacaggacaa gcaggaacaa
    29581 gacaaagtgg tcattgagca catagcgcag ataaccaaga cactccttac gaaaggcctg
    29641 ctttctcttt aaaaacaagt ctttcataga tcctcctctt tggtcagagc caagtaaata
    29701 tcattcaaac tagcctcagg catatcaaag gcttcacgta gttgcaggag attgcctttg
    29761 gcacgcacct ctcccttgtg aagaatgaca aaggcatcac acatcttctc cgccgaatcc
    29821 agcacgtggg tactcatgag aatagacttg cccttttgct tctccacttc caaaagctga
    29881 atcaaatcag aaatagccag cggatcaaga ccaaggaaag gctcatccac gatgaaaaga
    29941 cttggatcca ccacaaaagc acagataatc atgaccttct gcttcatccc ttttgaaaaa
    30001 tgaacaggga accagtctaa tttctgttcc aaacggaaca tttttaacaa gggctctact
    30061 cgttcgaaag ccactttttg ctcaataccg taagccatag caaccgtttc gatatgctct
    30121 ctgagggtca attcctcata cagactaggc gtctcaggaa tgtagccaat ctgcttgcgg
    30181 tagctagtcg catctccttg cagagtcagg ccattgatat tgatggagcc actataaggt
    30241 gccaacagac cgataatctc attgatcgtc gttgatttcc cagcaccatt gagaccaatc
    30301 aaaccgacca actgcccact ttcaacagta aaggacacat ctttcaaaac aggaacatga
    30361 acatagccac ctgtcaggtt tttaatttct aacatatttt ctccaaatct ggtataatgt
    30421 agctatatta tatcaaaatt cagtacagta gaggtagatt ttatgtcaga ttgcattttt
    30481 tgtaaaatca tcgcagggga aattcctgct tcaaaagtat atgaagatga gcaggtcctt
    30541 gcctttcttg atatctctca agtaacacta ggacacacct tggtcgtgcc aaaagaacac
    30601 tatcgcaatc ttttggagat ggatgctacg agcgccagcc aactctttgc ccaagtacca
    30661 aaagtagctc aaaaagtcat gaaagtcact aaggctgctg gtatgaatat catttccaac
    30721 tgtgaagaag tcgctggtca aacagttttt catactcacg ttcaccttgt gcctcgctac
    30781 agtgctgacg atgacctcaa gattgatttt atcgcccacg aaccagactt tgacaaactt
    30841 gctcaagtcg ctgaaaccat caaaaacgct taaggagttg ctatgaaatt atccaaccta
    30901 ctgctatttg caggagctgc agccggaagt tatctggtta caaaaaatcg ccaaaccatc
    30961 acagatgaag tcttgaatac cactgaccgc gttcaagcta tcaaggacga tgtggatatt
    31021 atccaaaaca gcctgcaaat cattaaccag caaaaagaac ttatcaagga ataccaagaa
    31081 gacttgactt acaagtttaa ggtcttggaa aaggatatcc agactagact agctgtgata
    31141 aaagaaatgc agggaactga agataagtaa aaagagcccg acggctcttt tttaaaacgt
    31201 aaacaagtta tagtgaattg aatttagaat agcacatcat agtttctaaa gcattgttag
    31261 agattacttt aaaatcctct tatcaatttg ttcatattct atttcaatct actatatata
    31321 tcccatcaac tatgcatcat aatttaggta actcatactc aataaaaatc aaaaagcaaa
    31381 ctagaaagct aggcacagac tgctcaaaac accgttttaa ggttgtggat agaactgacg
    31441 aagtcagctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc agtaaccata
    31501 cccatgacaa gccgacgctg acgtggtttg aagaaatttt tgaagagtat cattcaccat
    31561 ttcacaacta aatcgaagac tttctgccgt aatgaaaaca cctgaaacag cttggtttca
    31621 gctgtccgga aactttaaga cttaggttca aagtttaggt atggaacttc gaaggaggtc
    31681 gctaccgtcc gtcattactt aggaaagtct taaaaaatct ataaagtaaa aagagcccga
    31741 cagctctttt tgtttatact caatgaaaat caaaaagcaa actaggaaac tagccacggg
    31801 ctgctcaaaa cactgttttg aggttgtaga tacgactcac atatatacgg caaggcaacg
    31861 ctgacgtggt ttgaagagat tttcgaagag tattattctc catcaaaggc atcttttata
    31921 tggtcaaaga agcctttttt ctttggattt actttcaagt caccagcagt cgcgaattct
    31981 ttcaaggcta ctttttggcg gtcgttcaag cctgtcggtg ttacgacatt aacagtaacg
    32041 tattggtcac caactgcacc gccacgaagg ctcggtgccc ccttactacg taggcggaat
    32101 ttcttaccag tctgagttcc ctctggaata accaattcaa catcaccgtg aacagttgga
    32161 atatctactg tatcaccaag agccgcttgg acaaagttga ggttgagatt gtagaagata
    32221 gtcgttcctt cacgttcaaa cttgtcgcta gcttctacag aaactactac atacaagtca
    32281 ccataaggtc caccgttaaa gcctgcttca ccttgaccag cgaggcgaat ttgttgacct
    32341 gtttccacac cagcagggat tttcacatgt acgctatgag cttgtttctc atgacctgtt
    32401 ccatgacagg ttgtacatgg atatttgatt tcttttcctc gaccgtgaca gacatcacag
    32461 gttacttggc gacgcatcat accaagagga gtctgcgtat cgacgttaat gacaccagcg
    32521 ccatgacagc gtccacaagt gactggactt gtccctggct tagcaccaga gccattacat
    32581 gtacgacagc cagcttcacg atgatactta acttccttct cagttccgaa gatagcttct
    32641 tcaaaggtca aattgacacg atactggaga tcatctcctt ggcgaggagc gtttggattg
    32701 cgcgaagaac cgcctccgcc gaagaaactt gagaaaatat cctcaaaacc accgaagcca
    32761 cctgccccat tgaaaccacc gaaaccacca gctccaccaa aaccaccatt ggcgcctgca
    32821 gcaccatact ggtcataggc agcacgtttt tggtcgtcac tcaaagtctc ataggcttct
    32881 tgaacttcct tgtacttgtc ctcagcacca ggctccttgt tgatatctgg gtgatatttt
    32941 ttggaaagct tacgataagc ctttttgatt tcgtctgccg aagcgttttt ggataccccc
    33001 agacgatcat aaaattcagt attgttcata caagatacca agaccgtaaa attcgactga
    33061 aaaataggaa atctgacgct ggagcgacgc tcctagacag atttctcttt tttccgagaa
    33121 tttaggtcgg gttcaattcc tttcttttat attgatgaga taatactcca gccactcggc
    33181 cctatccctt tttaccgaaa aacagaggct gggttgcaac ctctgtggtt agattattca
    33241 tttgaaataa aaatggttgt attatctcta gtagtaatct tcaaagacct tttagctaaa
    33301 tcaaactcaa atttattata gtgtgggtct tgtgttttat ataggtgtaa ctcctgacat
    33361 atatgatccg aaaagtaaat cataacatct ttaaacacag aagtgtggtc atatttactc
    33421 ttaccaacag ctacttctgt aaattcttta taaatgacat ttccatttct ttccacagtc
    33481 accagcattt ctgttggatt ataatccaaa ttaaataatt attcaaaaaa actttatctt
    33541 ttcatgataa tctgcaatgt cagaaagatt atttttatta acactcataa cactagcccc
    33601 ttatttttca gtaaactctc cgtctacgac gtcatcgcct gcgtttcctg ttgcttgtgc
    33661 gccttctgct ccttcttgag cttgttgcgc tgctgcggct tgttcgtaga gtttcacagc
    33721 aaggccttga gctttttcgt tcaacgcttc aagtttcgct ttcatgtcgt ccaagttgtt
    33781 gtcttcttga gctttcttaa ggtcatcaag ggcagcttgg gcagcgtcac gttctgcgtc
    33841 gaagccttta ccttcagttt ccttgattgt cttttcagtc gcaaagattg cttggtctac
    33901 ttcattacga aggtctactt cttctttacg tttcttatcg gcttcagcgt ttgcttctgc
    33961 atctttcatc atgcggtcga tttcttcgtc agtcaaacct gagttcgatt ggatgacaat
    34021 agtttgttct ttttgagttc caaggtcttt ggccttaaca gacacgatac cgttcttgtc
    34081 gatgtcaaat gttacttcga tttgaggaat tccacgaggt gcagctggga tatcagtcaa
    34141 ttggaagcgt ccaagagtct tgttatctgc tgccattggg cgttcacctt gaagaacgtg
    34201 gatatcaacg gctggttggt tgtctgctgc tgttgagaag acttgtgatt tagatgttgg
    34261 gattgtagtg ttgcgatcga taagttttgt aaatactcca cccattgttt cgataccaag
    34321 tgacaatggc gttacatcaa gaaggacaac gtccttgaca tcaccagtaa tcacaccacc
    34381 ttggatagcc gcacccatag caactacttc atcagggttt actgatttgt ttggttcttt
    34441 accagtttca gctttaacag cttcaacaac ggcagggata cgagttgaac caccaacaag
    34501 gataacttcg tcgatttctg acaagctcaa acctgcatct gaaagggctt gacgaactgg
    34561 aacttttgta cgttcaacaa ggtcacgagt caaatcatca aatttcgcac gagtcaaagt
    34621 catttccaag tgaagaggtc cagcctcacc tgcagtgata aatggcaagc tgatttgtgt
    34681 tgaagttaca ccagaaaggt ctttcttcgc tttttcagcc gcatctttca aacgttgcat
    34741 tgccatcttg tcagtagaca agtcgatacc gttttctttc ttgaattctg ctaccaagtg
    34801 gtcaatgatt ttttggtcaa agtcgtcacc accaagtttg ttgtcccctg cagttgacaa
    34861 tacgtcgaag acaccgtcac ccaattcaag gatagagacg tcgaatgtac caccaccaag
    34921 gtcaaatacc aagatttttt cttctttgtc agtcttgtcc aaaccataag caagagctgc
    34981 tgcagttggt tcgttaacaa tacgttctac ttcaagacca gcaattttac cagcatcttt
    35041 tgttgcttga cgttgagcgt cgttgaagta agccggaact gtgataacag ctttggttac
    35101 tttctcacca aggtagtctt cagcgtagcc tttcaagtat tgaaggatca tagctgagat
    35161 ttcttgtgga gtgtattctt ttccatttgc agaaactttt tcagaagttc ccatcttaga
    35221 tttgatagag ataactgtat ctgggtttgt gactgcttga cgttttgcag catcaccaac
    35281 gatgatttct ccgtttttga atgagactac agatggagtt gtgcggtttc cttctgggtt
    35341 tgcgatgatt ttgctttcag ttccttcaag aactgcaact gctgagtttg ttgtacctaa
    35401 gtcaataccg ataattttag acatgtgttt ttctccttgt tagttaattt tctatttttc
    35461 tttttgatac tcttcttaag tggcgacgcc gtcagagctt gacttcgtca atctctttga
    35521 ctaaactttg agcctaaggt ctcaaaattt gcgcaaatag caccacggcg aagagtatcg
    35581 tctttggttc gcttcgctca ctattacaaa gccaaacata ttttatcttt cttcatagtt
    35641 tattgtcgtt tcggacaagt tttctattat gtaaattgcg acacgaggag tcgaaatcga
    35701 ttttatttcg acgacgagtt agtaaggaag ctaggcaaac gtcatagcga ttgccgtttt
    35761 ctgacgagtt gctttagcta ccgtcagaat tgccgaatcg aacacgccct aagctctgag
    35821 taaaaaagat agattttctt gtgttcatcg aacacttcgt caatctccta tttttactgc
    35881 gagcttttta cgagctttgt atcttagtta tacaccacta ccattgctgg gcgtaggatg
    35941 cggtcatgga gtttgtagcc tttttgaaag acttgggcga tggtatctac tgggtgttca
    36001 tcgtctgctg ggagagtttg gatggccata tggtagttat ggtcaaattc gccatctgct
    36061 gcgatttctt caattccttc ttctttcaaa gcgtgaatca agctttcttg caccatcgcc
    36121 aagcccttct tcacatcatc tgtcaaacct tcaactgcaa gtgcacgctc aaggttgtca
    36181 agagatggta agattgcttt tgccaagtcc tggctacgat aacgttgcaa gttttgacgt
    36241 tcttcattgg cacggcgttg gatattttgc atttctgcat gagcgcgaag atatttgttt
    36301 tcgaactcat ctgcacgttc atttgccaag tccaactcag acttttcagg agttgtttct
    36361 tcagctgttt ccacaacttc ctcttcttga acttcttcta cttcttcatt ttttatatct
    36421 tgggccatgt tcgcctcctt taatgatttc aatcttaatg tacttcgtaa tgattactgc
    36481 tgaggtagcg gtaaaaatct gtcaacttca tggtcaaaac acggttgacc acattgactt
    36541 gattgattag ctgttggtaa tccagattaa ctggaccgat aatggctaga attccaactc
    36601 ctcgataagg aatgaggaac ttactactaa tcaccgctag gtcagctaaa caggactctt
    36661 gaccgtctgc aacacgaaca ttttgcatct gatcctcacg caacccctca cgaatctcca
    36721 aggccacctt ttgcggttgg tcaaagaact gataggcttc tagattggca aaattcaaga
    36781 gattgacctt gcccgccatc acaatgtttt cgttgaacat ttccttaaag atgtgttcaa
    36841 agagatcgat gacattatcc gttgttgtaa agtaacgctg gataatctgc ggaatctccg
    36901 tccgaatctt gtagtgaata tctaaaacgg tgtgaccgag gaaacgttcc tgaatgatgc
    36961 tcttcagttt cagcaaatcc tcctgcaaga agttccttgg aatcagaaac tgactagtaa
    37021 ccgttcgcga ctcgtctagg gtaaataccg ccaaggctgt atgttgcccc aaaacaacga
    37081 tatcaaaggc tgtcaaacgt tgcctgctcg gctcaacatc cagtgccact accgtacagc
    37141 cactcaggtc tgttagcaag ttagcagcct cttgcagaat atcctccaat ttgaagaatt
    37201 cctgatcaaa ggctttgaca atctcatata cctcattttc agccagccgg tgaaaatcca
    37261 gtgagtgttt cacatagtac tgaaaaccag caacacttgg catccgaccg cttgaagtat
    37321 gagccttctc aagcaaccct tgtttttcta gttccgccat gtcattacga atggttgcac
    37381 tgctagagtt aatagactct tgcaaggctt ttgatccgac aggttcgtgc gttttggtaa
    37441 agatgtcaat aatcagattt aaaatatcct gctgacgctc tgtaaccatc tcatcactcc
    37501 tctctgtctg atcaattagc actcaatgca tccaagtgct aacttatgat tctattatac
    37561 acccttaaaa gagaatgtca agacaaaaac tcaaaaaatt agcactcttt tatcaagagt
    37621 gctaaaaatc aatctgaaga cctagaacct tgctattcat ctactcgata tttccttttt
    37681 agtctgaaat aaccatgaat tagataagaa atcagaaaag catataaaac aaaaataaga
    37741 aagaaagaca tagaaagatc ctctctaaac aaactcgtac aaacaacaag accacctaag
    37801 aaagtagttg tacaggaacg aagtctagat cgcataactt cccatttgag ccccttactc
    37861 atatagactt gatcctctgg aagtaaatca gaagacgatt tacgaagaat cgaacaagaa
    37921 cctgctccta tcaattccca acctctatct ctaaaatctt gcagttcatg cttatatttt
    37981 ttaagaaatc tagaatcata gatacggtag atgacatcgt ctggttgaca ttggtcaaaa
    38041 tagaacaaac caaaacgact cgttctatac ctccaacctt tcaaatgcat ctcatgtaaa
    38101 tattcttctt acttgtccaa atcaacaatg gtgaaaatcc gaaattctac tctgctattc
    38161 attgtcttac cccaaaatta gaaaacatgc ctggcgttat ttattagata attctttcca
    38221 cttttgactc aatctccaaa aaatataaga aatctgaatc gcaaaaacta tcaataaaac
    38281 ccaatctatt atgaaaatca aaaacacttt ccaactgaaa gaactacctc cagtgacaaa
    38341 ctttgagaaa aacggtagta gagctaaaaa gagaagtaaa ataggaagca tccgcattgt
    38401 taaaatccgt ttggcataaa aaaatcttta tttaaactaa aatattatat caaaatttac
    38461 gccagttttt gaacggctga tgtagatatt ttatactttc aaaatgttta aatgtgatta
    38521 tttatttttg aaaaatagct caccagcccg actgaaagtg cttatagaat gataataagt
    38581 cgcctgccga aaacagcgga aaatagcggt gttatgcgga gataatctga cgcgatgcga
    38641 aagtatattg catacttatt ttcaacaatt tagcagagta tttttataag tgtgatataa
    38701 tagaagtata atttgttctg atagtttatt ttatggagaa gtagattttt agaatgcgga
    38761 gggttcaata tggttgagtt tataaagtct aagaaagaaa tgagtgagga ggatattaaa
    38821 gcaaatttca tcactcctgc tattgtatcc aaaggatgga aaaatggtga gcatatcgct
    38881 tacgaagaat acttcactga tggtcgaatt gaggttagag gagataaggc tcgtcgtaaa
    38941 gaaggaaaaa aatcagacta ttcactgtat taccaatttg gaactcgaat tgcaattgtt
    39001 gaggcaaagg ataataaaca cagcgttcga gcaggattac aacaagctat tgaatatgga
    39061 gagattttag atgttccatt tgtttattct tcgaatggtg atggctttat tgaacacgac
    39121 cgtatcacga gagaagaacg tgagctggag ttagacgaat tccctactcg tgaagaatta
    39181 ttttctcgta tgacgaagga aaaaggattg acgtacgaaa ttacagaagc tatctcaact
    39241 ccatactata cagacgcctt ctcaatgaaa acgccacgct attatcagca aatagctatc
    39301 aaccgtacta ttgaaacagt tgccagagga caaaaacgag taatgtttgt gatggcaaca
    39361 ggaacgggga aaacgttcat ggcttttcaa attattcatc gccttcgaaa agctggtttg
    39421 gctaaacgag ttttattctt agcggataga aacatcttag tagaccaaac gatggctgaa
    39481 gactttaggc cattcgaaaa ggtaatgacg aaaattacac caaaactttt gactgctcct
    39541 gaaaaattaa attcttttga aatttatcta gggctttatc agcaactaac tggtgaagat
    39601 ggaactgaaa cacattatca aaaatttgac aaagacttct ttgatttaat cgtaattgat
    39661 gaagcgcacc gtggttcagc taaggaaaac agtaactggc gtaagataat tgattatttc
    39721 agttctgcga cacagattgg gatgaccgct actcctaaag aaaccaagaa tgcttccaat
    39781 acggaatact ttggtgagcc aatctatact tatagtttaa aacagggaat cgaggatggt
    39841 tttttggctc catatcgtgt tatgagagtt aatttagatg tagatgtgga tggttatcgt
    39901 ccagaaactg gaaaagttga tgctaacgga caattaatag aagataggta ctacggcagg
    39961 aaagattttg ataaaaccat tgtcattgat gatagaacgc aaagagttgc caagtttgtt
    40021 tctgattata tgaagcaaaa caatgcacga tttgataaaa caattgtttt ttgtgttgat
    40081 attgaccatg ccgagcgaat gcgtgctgca cttgtaaaag agaatctaga cttagtccaa
    40141 gaagactatc gttatgtcat gcaagtaact ggtgacaacg ctgaaggaaa agctcaactg
    40201 gataacttta tggatgtcaa ttctaatttt cccgctattg taacaacgtc taaattatta
    40261 acgacaggag ttaatgctaa aacatgtcgt ttgattgttt tagactctaa tatccaatcc
    40321 atgactgaat ttaaacaaat tattggtcgt ggcacacgtc tttatcctca aaaggggaaa
    40381 gaatttttta cgattattga ttttcgaaat gttaccaatt tgtttgctga ccctgatttt
    40441 gatggtgatc cagtgaaggt gctggaaaca ggtgcgaaaa cagtcagtgg ttctacgccc
    40501 ggtttcgtaa gtgaggaagg tgacccagta gaaaaatata tcgttacaga caagcaggtt
    40561 accattctta attctactgt tcaagtattg gatgaaaacg ggaaactgat taccgaaagc
    40621 ctgaccgact acactcggaa gaatatctta ggtagctacg ccactttgaa cgattttatc
    40681 acagtttggc atacggcgga taaaaagaag cttatcttag acgaacttta taaaaaagga
    40741 gtttatctag atgctattcg agagtcggag ggaatatcag aacaagaaat cgatgatttt
    40801 gatttactcc taaaacttgc ctatggtcaa aaagaattaa ccaaaacgga acgtatcaat
    40861 aagctcaaac aaagcggata tttatataaa tatagtgagg aagcgcgtgc tgttttggaa
    40921 attttactga acaaatacat ggataaaggt attggagaac tcgaaagcat tgaaacatta
    40981 aaacttccag aatttcagat atatggtgga accttcaaaa tcatcaatac ttattttgga
    41041 gataaaaaac gatatttaca agcaattaaa gaattggagc aagagctatt tacagtagct
    41101 taatgaaagg aaagtatgtc aattacatca tttgtaaaaa gaattcaaga tatcactcga
    41161 aacgatgctg gtgttaatgg tgatgctcaa cgtattgagc aaatgtcttg gttattattc
    41221 ttaaaaattt atgatagccg tgaaatggtt tgggaattag aagaagacga gtatgagtca
    41281 attatcccag aggaattaaa atggcgaaat tgggctcatg ctcaaaatgg ggaacgggta
    41341 ttgacaggcg atgaattact tgattttgtc aataacaagt tattcaaaga gttgaaagag
    41401 cttgaaataa cttcaaatat gcctattcga aaaacgattg ttaaatcagc ttttaaagat
    41461 gcgaacaact atatgaaaaa tggcgtcttg ttacgccaag tcatcaatgt tattgatgaa
    41521 gttgatttca atagccctga agatcgtcat tcgtttaatg atatttacga aaaaattctt
    41581 aaagatattc aaaatgctgg gaactcagga gaattttata caccacgtgc agcgactgat
    41641 tttattgccg aagttcttga cccaaaactt ggagaatcaa tggcagacct tgcttgcgga
    41701 acaggaggct tcttgacttc gactctgaac cgtttaagta gtcaacgtaa aactagtgaa
    41761 gataccaaaa aatataatac agctgttttt ggtattgaaa agaaagcatt tcctcatctt
    41821 ttagcagtta caaatctgtt tcttcacgaa attgatgacc ctaaaattgt tcatggaaat
    41881 actttagaga aaaatgttcg tgaatatacg gatgatgaaa aatttgacat tattatgatg
    41941 aatccacctt ttggagggtc agaattagaa acaataaaaa ataactttcc agcagaatta
    42001 cggagttctg aaacagctga tttatttatg gctgtcatta tgtatcgttt gaaagaaaat
    42061 ggtcgtgttg gagttatttt acctgatggt tttctatttg gtgaaggtgt aaaaactcgc
    42121 ttgaaacaaa aactggtaga tgagttcaac ttgcatacga ttattaggtt gcctcatagt
    42181 gtctttgcac cgtatacagg aatccatacg aacattcttt tctttgataa aacaaagaaa
    42241 acagaagaaa cttggtttta tcgtttagat atgccagatg gttataaaaa tttctcgaaa
    42301 actaagccga tgaagtcaga acacttcaat cctgttcgtg actggtggga aaatcgtgaa
    42361 gagattctgg aaggtaagtt ctacaaatct aaatcattta cacctagtga attggctgag
    42421 ttgaattata atttagacca gtgtggtttt ccaaaagagg aagaggaaat cttaaatccc
    42481 tttgagttga ttcagaatta tcaagcggaa agagcaactt taaatcataa gattgataat
    42541 gtattagctg atattttgca gttgttggag gacaaataat gacaccagaa caacttaagg
    42601 caagtattct ccaaagagcg atggaaggga aattagtgcc gcaaaatccc aatgacgaac
    42661 ctgcaagtga attattaaag agaattaaag ctgaaaaaga aaaacttatc agtgaaggaa
    42721 aaatcaaacg agataaaaag gaaactgaga tatttcgtgg tgatgatggg aaacattatg
    42781 ggaagtttgc tgatggaagt actcaagaaa ttgatgttcc ttatgatatt cctgatactt
    42841 gggagtgggt gaggataaaa tcaatttatt ggaattttgg gcaaaataag ccagagaaat
    42901 cctttaggta tatagatacg tctagtattg atagaaaaaa gaacataatc aactacaaaa
    42961 atctacaata tctttcacct gaacaagcgc cttcccgtgc tagaaaatta gtttcgcaga
    43021 atagtgtctt attttcaaca gttagaccat atctaaaaaa tattgctgta gttagagaac
    43081 ttaaagagta tttgatagct agtacagcat ttattgtttt ggatacttta cttaacgaaa
    43141 catatttgaa atattattta ttatcagata attttatcaa tcgtgttaat aataaatcta
    43201 ctggaacaag ttatcctgca atcaatgatt ataattttaa tctattatta attgctctcc
    43261 ccccactatc cgaacaacaa cgaata
//
LOCUS       DAVKMU010000015        40282 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKMU010000015 DAVKMU010000000
VERSION     DAVKMU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.015"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            19..89
                     /locus_tag="VW343_000888"
     tRNA            19..89
                     /locus_tag="VW343_000888"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51..53,aa:Gly,seq:tcc)
     gene            124..197
                     /locus_tag="VW343_000889"
     tRNA            124..197
                     /locus_tag="VW343_000889"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:158..160,aa:Ile,seq:gat)
     gene            280..369
                     /locus_tag="VW343_000890"
     tRNA            280..369
                     /locus_tag="VW343_000890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Ser,seq:tga)
     gene            380..453
                     /locus_tag="VW343_000891"
     tRNA            380..453
                     /locus_tag="VW343_000891"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:414..416,aa:Met,seq:cat)
     gene            457..529
                     /locus_tag="VW343_000892"
     tRNA            457..529
                     /locus_tag="VW343_000892"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Phe,seq:gaa)
     gene            542..622
                     /locus_tag="VW343_000893"
     tRNA            542..622
                     /locus_tag="VW343_000893"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:576..578,aa:Tyr,seq:gta)
     gene            627..697
                     /locus_tag="VW343_000894"
     tRNA            627..697
                     /locus_tag="VW343_000894"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:659..661,aa:Trp,seq:cca)
     gene            712..784
                     /locus_tag="VW343_000895"
     tRNA            712..784
                     /locus_tag="VW343_000895"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:745..747,aa:His,seq:gtg)
     gene            795..866
                     /locus_tag="VW343_000896"
     tRNA            795..866
                     /locus_tag="VW343_000896"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:827..829,aa:Gln,seq:ttg)
     gene            876..959
                     /locus_tag="VW343_000897"
     tRNA            876..959
                     /locus_tag="VW343_000897"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:910..912,aa:Leu,seq:caa)
     gene            1066..2343
                     /locus_tag="VW343_000898"
     CDS             1066..2343
                     /locus_tag="VW343_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9755471.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2419..3903
                     /locus_tag="VW343_000899"
     CDS             2419..3903
                     /locus_tag="VW343_000899"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9755472.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIALLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNMQGQYKLTDFDAEILELFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTTVAAADMQAAVEAYLGL"
     gene            3964..5244
                     /locus_tag="VW343_000900"
     CDS             3964..5244
                     /locus_tag="VW343_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9755473.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5248..5868
                     /locus_tag="VW343_000901"
     CDS             5248..5868
                     /locus_tag="VW343_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9755474.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6034..7176
                     /locus_tag="VW343_000902"
     CDS             6034..7176
                     /locus_tag="VW343_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9755475.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPGEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7582..7920
                     /locus_tag="VW343_000903"
     CDS             7582..7920
                     /locus_tag="VW343_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9755476.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            7931..8289
                     /locus_tag="VW343_000904"
                     /pseudo
     CDS             7931..8289
                     /locus_tag="VW343_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
     gene            8396..9013
                     /gene="pcp"
                     /locus_tag="VW343_000905"
                     /pseudo
     CDS             8396..9013
                     /gene="pcp"
                     /locus_tag="VW343_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(9041..9898)
                     /locus_tag="VW343_000906"
     CDS             complement(9041..9898)
                     /locus_tag="VW343_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9755477.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVFFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10028..11170
                     /gene="tgt"
                     /locus_tag="VW343_000907"
     CDS             10028..11170
                     /gene="tgt"
                     /locus_tag="VW343_000907"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9755478.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11268..13085
                     /locus_tag="VW343_000908"
     CDS             11268..13085
                     /locus_tag="VW343_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9755479.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTV
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13238..14389
                     /gene="nagA"
                     /locus_tag="VW343_000909"
     CDS             13238..14389
                     /gene="nagA"
                     /locus_tag="VW343_000909"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9755480.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14552..15610
                     /locus_tag="VW343_000910"
     CDS             14552..15610
                     /locus_tag="VW343_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9755481.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15761..16126
                     /locus_tag="VW343_000911"
     CDS             15761..16126
                     /locus_tag="VW343_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9755482.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16202..17143
                     /locus_tag="VW343_000912"
     CDS             16202..17143
                     /locus_tag="VW343_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9755483.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17091..18107
                     /locus_tag="VW343_000913"
     CDS             17091..18107
                     /locus_tag="VW343_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9755484.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18109..18435
                     /gene="comGC"
                     /locus_tag="VW343_000914"
     CDS             18109..18435
                     /gene="comGC"
                     /locus_tag="VW343_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU9755485.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQVKAYKEYNDKNGGA
                     NRKVND"
     gene            18404..18832
                     /locus_tag="VW343_000915"
     CDS             18404..18832
                     /locus_tag="VW343_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9755486.1"
                     /translation="MEEQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLPVPKGIQAPSGQSIIF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18837..19097
                     /locus_tag="VW343_000916"
     CDS             18837..19097
                     /locus_tag="VW343_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755487.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQININGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19060..19521
                     /locus_tag="VW343_000917"
     CDS             19060..19521
                     /locus_tag="VW343_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9755488.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVRFRFQFQKGLEREFIYRVEKEKS"
     gene            19499..19912
                     /locus_tag="VW343_000918"
     CDS             19499..19912
                     /locus_tag="VW343_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9755489.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            20049..20636
                     /locus_tag="VW343_000919"
     CDS             20049..20636
                     /locus_tag="VW343_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9755490.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKEILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQAHLKAEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            20697..21650
                     /locus_tag="VW343_000920"
     CDS             20697..21650
                     /locus_tag="VW343_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9755491.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVELFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21701..22891
                     /locus_tag="VW343_000921"
     CDS             21701..22891
                     /locus_tag="VW343_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9755492.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23040..23411
                     /gene="rnpA"
                     /locus_tag="VW343_000922"
     CDS             23040..23411
                     /gene="rnpA"
                     /locus_tag="VW343_000922"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9755493.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23386..24210
                     /locus_tag="VW343_000923"
     CDS             23386..24210
                     /locus_tag="VW343_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9755494.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24229..25215
                     /locus_tag="VW343_000924"
     CDS             24229..25215
                     /locus_tag="VW343_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9755495.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25266..25889
                     /locus_tag="VW343_000925"
     CDS             25266..25889
                     /locus_tag="VW343_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9755496.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLEKQAKILDIPTVKGIYYLAYSDGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26020..27477
                     /locus_tag="VW343_000926"
     CDS             26020..27477
                     /locus_tag="VW343_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9755497.1"
                     /translation="MEVISSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFIFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27500..27781
                     /locus_tag="VW343_000927"
     CDS             27500..27781
                     /locus_tag="VW343_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9755498.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27858..28343
                     /locus_tag="VW343_000928"
     CDS             27858..28343
                     /locus_tag="VW343_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9755499.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28360..29025
                     /locus_tag="VW343_000929"
     CDS             28360..29025
                     /locus_tag="VW343_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9755500.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            29029..29892
                     /locus_tag="VW343_000930"
     CDS             29029..29892
                     /locus_tag="VW343_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9755501.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29894..30598
                     /locus_tag="VW343_000931"
     CDS             29894..30598
                     /locus_tag="VW343_000931"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9755502.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIIVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30775..32448
                     /locus_tag="VW343_000932"
     CDS             30775..32448
                     /locus_tag="VW343_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9755503.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32560..33651
                     /gene="ulaG"
                     /locus_tag="VW343_000933"
     CDS             32560..33651
                     /gene="ulaG"
                     /locus_tag="VW343_000933"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9755504.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33765..35741
                     /gene="tkt"
                     /locus_tag="VW343_000934"
     CDS             33765..35741
                     /gene="tkt"
                     /locus_tag="VW343_000934"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9755505.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35858..36157
                     /gene="yajC"
                     /locus_tag="VW343_000935"
     CDS             35858..36157
                     /gene="yajC"
                     /locus_tag="VW343_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9755506.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36204..36632
                     /locus_tag="VW343_000936"
     CDS             36204..36632
                     /locus_tag="VW343_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9755507.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEILYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36634..37044
                     /locus_tag="VW343_000937"
     CDS             36634..37044
                     /locus_tag="VW343_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755508.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37345..39996
                     /gene="adhE"
                     /locus_tag="VW343_000938"
                     /gene_synonym="adhC"
     CDS             37345..39996
                     /gene="adhE"
                     /locus_tag="VW343_000938"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9755509.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12216 a         7208 c         9079 g        11779 t
ORIGIN      
        1 ccgttaactc ccattttagc gggtgtagtt tagtggtaaa actacagcct tccaagctgt
       61 tgtcgcgagt tcgattctcg tcacccgctt tgaactttgt tctttgtacc aagtttttga
      121 cttgggcgcg tagctcaggt ggttagagcg cacgcctgat aagcgtgagg tcggtggttc
      181 gagtccactc gtgcccatag tgtttagtcc attactaggg gattggaata ttatctgttc
      241 actaagagga cacgggcttg ttcccgtata aactattttg gaggattacc caagtccggc
      301 tgaagggaac ggtcttgaaa accgtcaggc gtgtaaaagc gtgcgtgggt tcgaatccca
      361 catcctcctt ttatattaac gcgggatgga gcagctcggt agctcgtcgg gctcataacc
      421 cgaaggtcgt aggttcaaat cctgctcccg caataaggct cggtagctca gttggtagag
      481 caatggattg aagctccatg tgtcggcggt tcgattccgt ctcgcgccat ttatatattt
      541 tggaagggta gcgaagaggc taaacgcggc ggactgtaaa tccgctcctt cgggttcggg
      601 ggttcgaatc cctccccttc cattttacgg gcatagttta aaggtagaac taaggtctcc
      661 aaaaccttca gtgtgggttc aattcctact gcccgtgtta atagaattat ggcgggtgtg
      721 gtgaagtggt taacacacca gattgtggct ctggcatgcg tgggttcgat ccccatcact
      781 cgcctatttt atattggggt atagccaagc ggtaaggcaa gggactttga ctccctcatg
      841 cgttggttcg aatccagcta ccccagttac tatttgccgg cgtggcggaa ttggcagacg
      901 cgctggactc aaaatccagt gtccgcaagg acgtgccggt tcgaccccgg ccgccggtat
      961 agtatagtgt taggaacgtt gttattcttc gttccttttt tatattattt ttggtataat
     1021 tatagttatt caaattttgt ttagattgag aaagtgtagg gtagtatgtc ttgttctatc
     1081 gatttattaa aacatcggta tttgaaaaat attaaagaaa atcctgaatt gtttgtcgga
     1141 attgagttgg agtatcctgt tgcaagttta gaaggggatg ctacagatgt tgaagttata
     1201 aaggatctat ttcattattt agtttctact ttggatctca ccgtagcaaa ggtagatgat
     1261 tttggcaatc tgatccagtt agtagatccg ataagtcagg atgctatttt atttgaagtt
     1321 tcctatacaa cgattgagtt tgcatttggt aaggctgaaa cgattcaaga ggtcgaaaat
     1381 cgtttcaata attatatgaa tgtaattcag agaaagttag ctgaatcaaa tcatgctatt
     1441 gttggctgtg gtatccatcc caactgggat aaaaatgaga attgtccagt ggcttatcca
     1501 cgctatcaga tgttgatgga ttatttgaat ttgagtagaa atattattaa atcagattta
     1561 catcatttcc ctgaatatgg tacttttatc tgtgggagcc aggttcagct ggatatttca
     1621 aaaaccaact acttacgggt gattaatgct tttactcaaa ttgaagcggc taaggcttat
     1681 ttatttgcaa actctgaatt ttcgggtgcg gattgggata cgaaaatttc aagggatatt
     1741 ttctgggaag aatctatgca tggtatctat ccagagaatg ttggggtcaa tgctagactc
     1801 cttaatgatg aagctgattt ttttgactat ctaaatcatt ctgcgatttt tactgcggaa
     1861 cgtgatgggc agacctatta tttttatcct attcaggctg gggactattt ggctacgcct
     1921 gaaatccaag catttgctct gaatggggat gaggttatta tttcccccca agagaaggat
     1981 tttgaaactc atcgtagtta ccagtaccaa gacttaacga ctcgaggaac agttgagttt
     2041 cgtagtgtgt gtacacagcc acttgatagg acttttgctt ctgcagcttt tcacttggga
     2101 ttattggtta atttagacaa gttagaagct tacttagaaa cagcaccttt ctttaaagta
     2161 tttggttatg attacaagtc tttaaggaga caattttcta agaaaaatct tacagatgag
     2221 gaagaaacta cgattattga attttccaaa gacttactcc tactagctga ggagggacta
     2281 gtggtgagaa ataaggaaga aatgacctat ttacagcctt tgagagaaga attgagccta
     2341 taatttctct tataaaggga gaattttctg aaaaatcatg atataatgga cgagactata
     2401 gataaaggat agagagtaat gacattagtt tatcaatcaa cgcgtgatgc caacaataca
     2461 gtaactgcca gccaagcaat tttgcaaggt ttggcgacgg acggcggttt gtttacaccg
     2521 gttacttatc caaaggtaga tttggacttt gacaaattga aagatgcttc ttaccaggaa
     2581 gttgctaagc tagttttgtc agcattttta gatgacttta cagctgagga gttggactac
     2641 tgtatcaaca atgcctacga tagcaaattt gatactccag ctattgcact attagtgaaa
     2701 ttagatgggc aatacaattt ggaacttttc catggttcaa cgattgcctt taaggatatg
     2761 gccttgtcta ttttgccata ctttatgacg actgctgcta agaaacatgg tttggagaac
     2821 aagattgtta tattgacagc gacatctggt gacacgggga aagctgctat ggcggggttt
     2881 gcggatgtgc ctggtactga gattatcgtc ttttatccaa aggatggtgt cagcaagatt
     2941 caagagttac aaatgaccac tcagactggc gacaatactc atgttattgc tattgatggt
     3001 aactttgacg atgcgcaaac aaatgtgaag cacatgttta acgatgtggc tcttcgtgaa
     3061 aaattgacta ccaacaagtt gcaattttca tcagctaact ctatgaacat tggtcgtctg
     3121 gtgccacaaa ttgtttatta tgtttatgct tacgctcaat tggttaagac tggtgaaatt
     3181 gtagctggtg aaaaggttaa cttcacagta ccaacaggaa actttggaaa tatcttggct
     3241 gccttttatg ccaaacaaat tggtttgcca gttggtaaat tgatctgtgc ttcaaatgac
     3301 aacaatgttt tgacagactt ctttaaaaca cgtgtctatg acaaaaaacg tgagtttaag
     3361 gtaacaacca gcccatctat ggatatcttg gtatcttcaa acttggagcg cttgattttc
     3421 catcttttgg gaaataatgc tgaaaagaca gctgaactca tgaatgcttt gaacatgcaa
     3481 ggacaatata agttgacaga ctttgatgca gagattctgg agctctttgc agctgaatat
     3541 gcgactgagg aagaaacagc ggcagaaatc aagcgtgttt atgagttaga ttcttatatt
     3601 gaggatccac atacggcggt tgcctcagca gtttatagaa aataccaagc ggttactgat
     3661 gatgcgacta agacagtgat tgcttcaaca gctagtccct acaagttccc agtagttgca
     3721 gtagaagctg taactggaaa agcaggttta acagactttg aagccttggc tcaattacat
     3781 gaaatctcag gcgttgcagt gccaccagca gttgatgggc ttgagacagc tcctgttcgt
     3841 cacaagacaa ctgtagcggc tgctgatatg caagcagcgg ttgaggctta tttaggactt
     3901 taagatagag ggagtaaact cggttgggaa accaactgag tttcttttca tcaggaggag
     3961 agattgttta agaaaaataa agacattctt aatattgcat tgccagctat gggtgaaaac
     4021 tttttgcaga tgctaatggg aatggtggac agttatttgg ttgctcattt aggattgata
     4081 gctatttcag gggtttcagt agctggtaat attatcacca tttatcaggc gattttcatc
     4141 gctctgggag ctgctatttc cagtgttatt tcaaaaagca tagggcagaa agaccagtcg
     4201 aagttggcct atcatgtgac tgaggcgttg aagattacct tactattaag tttcctttta
     4261 ggatttttgt ccatcttcgc tgggaaagag atgataggac ttttggggac ggagagggat
     4321 gtagctgaga gtggtggact gtatctatct ttggtaggcg gatcgattgt tctcttaggt
     4381 ttaatgacta gtctgggagc cttgattcgt gcaacgcata atccacgtct gcctctctat
     4441 gttagttttt tatccaatgc cttgaatatt cttttttcaa gtctagctat ttttgttctg
     4501 gatatgggga tagctggtgt tgcttggggg acaattctgt ctcgtttggt tggtcttgtg
     4561 attttgtggt cacaattaaa actgccttat gggaagccaa cttttggttt agataaggaa
     4621 ctgttgacct tggctttacc agcagctgga gagcgactta tgatgagggc tggagatgta
     4681 gtgatcattg ccttggtcgt ttcttttggg acggaggcag ttgctgggaa tgcaatcgga
     4741 gaagtcttga cccagtttaa ctatatgcct gcctttggcg tcgctacggc aacggtcatg
     4801 ctgttggccc gagcagttgg agaggatgat tggaaaagag ttgctagttt gagtaaacaa
     4861 accttttggc tttctctgtt cctcatgttg cccctgtcct ttagtatata tgtcttgggt
     4921 gtaccattaa ctcatctcta tacgactgat tctctagcgg tggaggctag tgttctagtg
     4981 acactgtttt cactacttgg gacccctatg acgacaggaa cagtcatcta tacggcagtc
     5041 tggcagggat taggaaatgc acgcctccct ttttatgcga caagtatagg catgtggtgt
     5101 atccgcattg ggacaggata tctgatgggg attgtgcttg gttggggctt gcctggtatt
     5161 tgggcagggt ctctcttgga taatggtttt cgctggttat ttctacgcta tcgttaccag
     5221 cgctatatga gcttgaaagg ataggaaatg caaaaaacag cttttatttg ggatttagac
     5281 gggactttat tggactctta cgaagcgatt ttatcaggga ttgaggagac ttttgctcag
     5341 ttttctattc cttatgataa ggagaaggtg agagagttta tcttcaagta ttcggtgcaa
     5401 aatttgcttg tgcgggtggc agaagataga aatctggatg ttgaggtgct aaatcaggtg
     5461 cgtgcccaga gtctggctga gaaaaatgct caggtagttt tgatgccagg tgcgcgtgag
     5521 gtgctagctt gggcagacga atcaggaatt cagcagttta tatatactca taaggggaac
     5581 aacgctttta ccattctcaa ggacttgggg gtggaatcct attttacaga gattttaacc
     5641 agtcagagtg gctttgtgcg gaagccaagt ccagaagcgg ctacctatct gctagataag
     5701 tatcagttga attctgataa tacttattat ataggggatc ggactctgga tgtggaattt
     5761 gcccagaata gtgggattca aagtatcaac tttttagagt ctacttatga agggaatcac
     5821 aggattcaag cgttagcaga tatttcccgt atttttgaga ctaagtgata aaaagattgt
     5881 gtcagttttg tgacagagac ctaacaaact atttcaagta acctagtttg ttacaaggaa
     5941 tagacagttc tgttaaatag gcccgagagg gctttttttc tacatttttt gtgttatgat
     6001 agacaggtac taatttgaaa ggaatttgaa agaatgaaga aaagaatgtt attagcgtca
     6061 acagtagcct tgtcatttgc cccagtattg gcaactcaag cagaagaagt tctttggact
     6121 gcacgtagtg ttgagcaaat ccaaaacgat ttgactaaaa cggacaacaa aacaagttat
     6181 accgtacagt atggtgatac cttgagcact attgcagaag ccttgggtgt agatgtcaca
     6241 gtgcttgcga atctgaacaa aatcactaat atggacttga ttttcccaga aactgttttg
     6301 acaacgactg tcaatgaagc agaagaagta acagaagttg aaatccaaac acctcaagca
     6361 gactctagtg aagaagtgac aactgcgaca gcagatttga ccactaatca agtgaccgtt
     6421 gatgatcaaa ctgttcaggt tgcagacctt tctcaaccaa ttgcagaagc tccaaaagaa
     6481 gtagcatcaa gttcagaagt tacaaagaca gtgattgctt ctgaagaagt ggcaccatct
     6541 acgggcactt ctgtcccaga ggagcaaacg gccgaaacaa gcagtgcagt tgcagaagaa
     6601 gctcctcagg aaacgactcc aggtgagaag caggaaacac aagcaagccc tcaagcagca
     6661 tcagcagtgg aagcaactac aacaagttca gaagcaaaag aagtagcatc atcaaatgga
     6721 gctacagcag cagtttctac ttatcaacca gaagagacga aaataatttc aacaacttac
     6781 gaggctccag ctgcgcccga ttatgctgga cttgcagtag caaaatctga aaatgcaggt
     6841 cttcaaccac aaacagctgc ctttaaagaa gaaattgcta acttgtttgg cattacatcc
     6901 tttagtggtt atcgtccagg agacagtgga gatcacggaa aaggtttggc tatcgatttt
     6961 atggtaccag aaagttcaga attaggggat aagattgcgg aatatgctat tcaaaacatg
     7021 gccagccgtg gcattagtta catcatctgg aaacaacgtt tctatgctcc attcgatagc
     7081 aaatatgggc cagctaacac ttggaatcca atgccagacc gtggtagtgt gacagaaaat
     7141 cactatgacc acgttcacgt ttcaatgaat ggataaaccc gacttggtaa catcattttg
     7201 acgaatgaga tctagctttc gtgataggaa gcgattctcg ttcgtttttt ctttgtcata
     7261 ctcttcgaaa atctcttcaa accacgtcag ttttatctga aacctcaaag ctgtgctttg
     7321 agcaacctgc gactagcttt ctagtttact ctttgatttt cattgagtat caatttgaat
     7381 ggaaaatgga aagttattat cttgtaataa tccaagcaac attcttgcaa tctattttac
     7441 tttatatcac aattgattgg tcaactattg ataagtcaat ggataggagg aagaaatgat
     7501 agagattcaa gatttactgt atcaactccg cttgtctgag caagcgagta cgcaattgtt
     7561 tgaaaaaggc ttgggattag tttgacacgg tatcagattt tactgttttt gctggagcat
     7621 tctccttgta accaaatggc ggttcaggag cgtttgaaaa ttgatcaggc tgctttgaca
     7681 cggcatttca agattttgga aacggaaggt ttggtggagc gtcatcgtaa tcctgaaaat
     7741 cagcgggaag tgttggtaga ggctgcgaag tatgccaagg agcagttagt ggtgaatccc
     7801 cctctgcaac atatcaaggt taaggaagag atggaaagta tcttaacaga gtttgagaga
     7861 acagaactca gccgtttatt aaataaattg gttttgggta ttgaaaatat agaaatttaa
     7921 ggagaaatag atgtcaatta ttttaacaac gatcgttgct ttggagcatt tttacatttt
     7981 ttatttggaa agtattgcca cgcaatcaga tgcgactagt cgtgtattta atatggaaaa
     8041 ggaagaattg gctcatccgt cagtaagttc attgttcaaa aatcaaggaa tttataaggc
     8101 tctgctagga gtctttctct tgtatgtcat ttatttctca cagaatttag aaattgtgac
     8161 tatttttgtc ttatttgtga ttggtgctgc gacttacggc tctttaacag cggataaaaa
     8221 attattttga agcaaggtgg atcagctatt ttggccttga ttagtatttt actctttaaa
     8281 tacacttgaa ggttgattct aatctcgcta atccttttta atccagaata agggaaatat
     8341 gttatacttg tttttaagaa aaaagtctca ttgaattggt tttgaggagt tagaaatgaa
     8401 agtattagtg acaggttttg agcccttttg aggccattaa aggtttacca gctgaaatcc
     8461 atggtgctga ggtccgttag ctagaggtgc cgacagtttt tcacaaatct gctcaagtat
     8521 tggaagaaga gatgaatcgt tatcaacctg actttgtcct ttgtattggg caagctggtg
     8581 gaagaactag tttgacacct gaacgagtgg ccattaatca agacgatgca cgcatttctg
     8641 ataacgaaga taatcaaccg attgaccgtc ccattcgccc agatggtgct tcggcctact
     8701 ttagtagttt gccgattaaa gcgatggttc aagctataaa aaagagggct taccggcctc
     8761 tgtttccaat acggcaggga cttttgtctg cagccatttg atgtatcagg ctctctattt
     8821 ggtagaaaag aaatttccat atgttaaggc aggttttatg catattcctt atatgatgga
     8881 acaggtggtg aacagacaga ctattccagc tatgagttta gtggatattc ggagagggat
     8941 agaagcagca atcggcgcta tgatagaaca tggagatcag gaactcaagt tggtaggcgg
     9001 agaaactcat tgatagaaaa aagcttgagt ggaaaaacct tcaagctttt ggacgttttc
     9061 gagccaatac tgctcggtaa aacataattt tagtgcattg gatataaggt aggagtgaaa
     9121 aactagcaat gccaaaggta atccaattga ggaagtacca aggaagaagc tgtaaatcta
     9181 ggacaaagcg ctggaacttg tagcccttca taaaggaacg gctagttttt aggattcgtc
     9241 ttggtgggac ctgtcctagg tctagactat aacagagaag aaattccacc tgtgaatagg
     9301 cataatactg tggaatatag aggatatttc ctacaatgat caagatgaga cttgcaagaa
     9361 agtagagtcc aaagaccatg aggaaacgct cggtttcaac tgatgagaga tctagatttg
     9421 gaaactcagg atgtagggtg acgaattttt tggctaaaaa gctactataa aagaggaggt
     9481 aaatcccaag taaattaggg atactccata aaaagagata gaaacgtttg agaagtaggg
     9541 tcaaaaaggt ttgagaaaag cgctcctcat caaagagagc taggctgttt tttacagatg
     9601 gctccgtttt agaatctttc atgagtgtca gtgttgcata gacggaactg gtcaaaagaa
     9661 tagtcccgat aaagaagact agtagaggaa agaggtaggc ttgaagtatt tggccaagta
     9721 tgctgaaaaa tggctgttct aaaacagtcc cgtggatccg agataaggga ttaagaaaac
     9781 tagataagat gaccagcata ctgggaagga tatagaggag aaagagacgg ggggtgtcag
     9841 cctgaaaatg ttttgactcc tgacgaattg tttttaaatc aatttttgga tagttcattc
     9901 tcttattata ccatagttct tatacatagt tcgtgacagt tcctactttt tttgataaaa
     9961 tcatacagtg tgtccttggg cacactgtat gaactgggac tgtctttccc agcttcggag
    10021 gtaaaaaatg tcagattcac caatcaaata tcgtttgatt aagaaagaaa aacacacagg
    10081 agctcgtctg ggagaaatca tcactcccca cggtaccttt ccgacaccta tgtttatgcc
    10141 agttgggaca caagccactg tcaaaactca gtcacctgaa gaattgaagg agatgggttc
    10201 gggaattatc ctatcaaaca cctatcatct ctggcttcgc cctggagatg aactcattgc
    10261 acgcgctggt ggtctccaca agttcatgaa ttgggaccag cctatcttga cagatagtgg
    10321 tggttttcag gtttattctt tagcagatag ccgtaatatc acagaagaag gagtaacctt
    10381 taaaaatcat ctaaatggtt ctaagatgtt cctatcccca gaaaaagcca tctctattca
    10441 gaataatctg ggttcagaca tcatgatgtc ctttgatgaa tgtcctcagt tttatcaacc
    10501 ttatgactac gttaagaaat cgatcgagcg taccagccgt tgggctgagc gtggtttgaa
    10561 ggctcaccgt cgtccacatg accaaggttt gtttggaatt gtgcaaggtg caggatttga
    10621 agaccttcgc cgccaatcag ctcatgatct tgtcagcatg gatttctcag gctactctat
    10681 cggtggtttg gcagtgggag aaacccatga agagatgaat gcggtcttgg actttacaac
    10741 tcaactgcta cctgaaaata aacctcgtta tctgatgggt gtgggagcgc cagatagctt
    10801 gatcgatggg gtcattcgtg gggtggatat gtttgactgt gtcttaccga ctcgaattgc
    10861 tcgtaacggg acttgtatga ccagtcaagg acgtttggtt gtgaaaaatg cccagtttgc
    10921 tgaggacttt acgccactgg atcctgagtg tgactgctac acatgtaata actatacacg
    10981 cgcttacctt cgtcacctgc tcaaggctga tgaaaccttt ggtatccgct tgactagcta
    11041 ccacaatctt tacttcttgc ttaacctgat gaagcaagtg cgacaagcca tcatggatga
    11101 caatctcttg gaattccgtg agtattttgt ggaaaaatat ggctataata agtcaggacg
    11161 taatttctaa aatggaattg atataaaaaa atcctaagtt ttctcttagg atttttcttc
    11221 tttttttgat agaataaagt gtacaatgaa aggaagaata aactcgtatg cgcattaaat
    11281 ggttttcctt gattaggatt ataggtttac ttttggtact cttgtatcac ttctttcaga
    11341 cgatctttcc tggaggattc ttcggggtag atgtcttttt cacattttca ggtttcctga
    11401 ttacagctct actcattgaa gaattttcta agaacaatga gattgatttg ataggatttt
    11461 ttagaagacg cttttatcgg attgtgccac ctgtggtttt gatggtcttg gtgaccatgc
    11521 cttttacttt cttggttcgc caagactatg ttgctggaat tggtggccag attgcgggcg
    11581 tcttaggctt tatgaccaac ttctatgaac tcctaacagg tgggagttat gaatctcagt
    11641 tcattcctca tttgtttgtt cataattgga gtttggcagt tgaggttcac tactatattc
    11701 tttggggatt ggcagtttgg ttcttatcca aacaagctaa atcaaatggt cagttgaagg
    11761 ggatggtctt tctcttatct gctgctgcct tcttgatcag tttcttctcc atgtttattg
    11821 gtagttttct agtgacctct tattcctctg tttatttctc cagtttaact catgtctatc
    11881 cattcttttt gggaagtatg ttagcaacta ttgtaggcgt tcgtcagacg acttccctcg
    11941 tcaagcagtt ggataaaatc tgggatttac gaaagacttt ggtagttttt ggaggaggct
    12001 ttggtttcct agttcttttg actttctttg tcaaattcac ttatcttttt gcctatctta
    12061 tcggcttctt gcttgccagt cttgcagctc ttgccatgat tctggcggcg cgtgtcttac
    12121 atgaaaagac acatcatata caggagccga agattatcag ctttttagcg gatactagct
    12181 atgcggttta tcttttccat tggcctttct atatcatttt ctcacagttg acatcaaatc
    12241 ttcttgctgt attactgact ctgatttgtt cttatggctt tgccagtctg tcattctatg
    12301 tattggaacc ttggattgca ggcaagaaca cacctatttt acaaactctt cgtcccctgc
    12361 cttatattca cgcaattctt gcagcaggta caggaatctt gaccttcatt gtctgcatgg
    12421 tgaccttgtt ggcaccacaa gtgggagcgt ttgagacaga cttgactgtc aatggcttga
    12481 agcaagctgc aacaaatatt ggccagacca aggtgatggc agaacgggca gatgcaaaca
    12541 gtttgggaat tgctgatggc actatgttaa ttggtgactc agtggcttta agggcaaata
    12601 cagcactaca gacagttctt cctggagcac agattaacgc gcaggtcagc gtaacaacca
    12661 agaccgcaaa tgaaatcatg ctaaataata gccagaataa atttttacct aagacggtgg
    12721 tcattgcgac tggggtaaat aatcctgaga attacaagga tgactgggac agtatcgtga
    12781 aaaatcttcc taagggacac catatgattt tggtgactcc ttatgaggga gataagacaa
    12841 aagagaccta tgccatcgtt gagaaggctg ctgcctatat gagagaattg gcagagaaga
    12901 caccttacat tacgatagca gattggaatc aagttgcgaa agagcatcca gaaatttggg
    12961 caggaacaga ccaggttcat ttcgggagtg agagtagcac tatcgaagca ggagcaaaat
    13021 tgtatgcaga tacgattgcc acagctttgc agacagctca agacaagccg gttaaatcaa
    13081 aataacttgt attaaaaaag agaagagttg gagaaatcca gctcttttaa aatatctcca
    13141 aaaaataggt ctataccatt tacaaataaa aaagaaaggt ttataatgta attgacataa
    13201 taaattgtag aatcaatctt ttaaggaggt taacattatg cctaactata ttaaagcgga
    13261 tcagtttttc tacccacacg gagttcgtcg aggtggttac ttggaacttg tggacggcaa
    13321 gtttgggaaa catgtagagc agattcctga aggggctgag gtgattgact atacaggtta
    13381 tagcattgcc ccaggtcttg tggatactca tattcatgga tatgcaggtg tagatgtgat
    13441 ggacaacaac attgaaggta cattgcatac tatgagtgaa ggacttctta gtaccggtgt
    13501 taccagtttc ttacccacaa ctttaacagc cacttatgag caattgcttg cagtcactga
    13561 aaatcttgga aaccattata aagaagcaac aggtgctaag attcgtggga tttattatga
    13621 aggtccatat ttcacagaaa cttttaaggg ggcacaaaat ccaacttata tgagagaccc
    13681 gggtgttgag gagtttcatt cttggcaaaa agcggcaaat ggcttgctta ataaaattgc
    13741 ccttgcacca gaacgtgatg gggtggaaga ctttgttcgt acagttacgg gcgaaggtgt
    13801 gacggttgct cttggacatt caaacgcgac ttttgatgaa gccaaaaaag cagtcgatgc
    13861 tggagcgagt gtttgggtgc atgcctacaa tggaatgcgt gggttgactc accgtgaatt
    13921 gggtatggtt ggagccatgt accaattgcc acatacctat gcagagttga tctgtgatgg
    13981 tcaccacgta gatccaaagg cttgcgaaat tcttatcaaa caaaaaggaa cagaaaacat
    14041 cgctcttatc acagactgta tgacagctgg gggattggaa gacggagatt atatgttggg
    14101 agaattccca gttgtcgttg caaatggaac tgcacgcctc aaatcgacag gtaacttggc
    14161 aggttctatc ctcaaactca aagatggttt gaagaatgtg gtcgaatggg gaattgcgaa
    14221 tccgcatgaa gcagtcatga tggccagctt caacccagct aaatccgttc acatcgatga
    14281 cgtctgtggc caaatccgtg aaggctacga cgctgacttc atcgtattag ataaagattt
    14341 ggaattggta gcaacctacc tagatggcgt gaaacgttat caagcataag agagaaagca
    14401 gaagttagag actaacttct gcttttttat gcagggtact ttactggtaa ataaaaagtt
    14461 aaaaaaatca caaaaaaagc ttgaagaaac aaagaaaaag gcttacaatt gaactataaa
    14521 tattacaaat aaaaaaacgg aggagtgctt tatgaaagct tatacttatg ttaaaccagg
    14581 acttgcttct tttgttgatg tagacaaacc agttattcgc aagccaacag acgctattgt
    14641 gcgtattgta aaaaccacta tttgtggaac agacctccat attatcaaag gggatgttcc
    14701 tacttgccaa agtggtacca ttcttggcca cgaagggatt gggattgttg aagaagttgg
    14761 ggaaggagtt tccaacttca aaaaaggtga caaggtcttg atttcttgcg tctgtgcctg
    14821 tggtaaatgc tactactgta aaaaaggaat ttatgcccac tgtgaagacg aagggggctg
    14881 gattttcggt cacttgattg atggtatgca ggctgaatat ctacgtgtcc ctcatgcaga
    14941 taatactctt taccatactc cagaagactt gtcagatgaa gctttggtta tgctgtcaga
    15001 cattctgcct actggatatg aaattggtgt cttaaaaggg aaagtagaac ctggttgcag
    15061 cgtagccatt attggttcag gtccagttgg attggctgct cttttaacag cccaattcta
    15121 ttcaccagct aaattgatta tggtagacct agacgataac cgtttggaaa ctgccctatc
    15181 attcggtgcg actcataagg taaactcttc agaccctgaa aaagcgataa aagaaattta
    15241 tgatttgaca gatggtcgcg gtgtagatgt cgctattgaa gctgtaggga ttcctgaaac
    15301 atttgatttc tgtcaaaaga ttatcggtgt agacggaacg gttgccaact gtggtgtgca
    15361 tggtaaacca gttgaattcg atttagataa actttggatt cgtaacatca atgtaacaac
    15421 tggtttggta tctacaaata cgactccaca attgttgaaa gcacttgaaa gtcataagat
    15481 tgaaccggaa aaattggtaa ctcactattt caaactcagt gaaattgaaa aagcctacga
    15541 agtcttcagt aaggcagcag accaccatgc cattaaggtc attatcgaaa acgatatctc
    15601 agaagcctaa gtagtaaaaa tatttttgta cataagcaaa tagaaattca gtcatccatc
    15661 agatggctgg attttttatc aaaaaattaa gaaatgagca tatttctttc cttgtctggc
    15721 ggaattggtt ataatatacg gtacaaagga atgaatgaat atgtatcgtg ttatagaaat
    15781 gtacggagat tttgaaccgt ggtggttctt agaaggttgg gaagaagata ttgtagcaag
    15841 tagaaaattt gaccagtatt atgatgctct caaatactac aaaacttgct ggtttagatt
    15901 agaacaagaa tcccctcttt ataaaagtag aagcgacttg atgaccattt tttgggaccc
    15961 ggaagaccaa cgctggtgtg atgaatgtga tgagtattta caacaatacc attctttggc
    16021 tcttttgcag gatgagcagg ttatcccaga cgaaaaacta cgctcaggct atgaaaaaca
    16081 aaccagtcag gaaaggaatc gttcttgccg tatgaaatta aaatagagaa aagtaacttt
    16141 tttggagttg ctttttttat ttttctaacg ctttgcgaat agtataggtg aggaggtaag
    16201 tatggttcaa gaaattgcac aagaaatcat tcgttcggct cggaaaaaag gggcgcaaga
    16261 catttatttt gtccctaagt tagatgccta tgagcttcat atgagggtag gagacgagcg
    16321 ctgtaaaatt ggtagctatg attttgaaaa gtttgcagcc gttatcagtc actttaagtt
    16381 tgtggcgggt atgaatgtgg gagaaaaaag acgtagtcaa ctgggttcct gtgattatgc
    16441 ctatgaccag aagatggcgt ctctacgttt atctactgta ggcgattatc gggggcatga
    16501 gagtttggtt atccgtttgt tgcacgatga ggagcaggac ttgcattttt ggtttcagga
    16561 tattgaagaa ttaggcaagc agtacaggca acggggactc tatctttttg ctggtccggt
    16621 tgggagtggt aagacgacct tgatgcatga attgtccaag tcactcttta aaggacagca
    16681 agttatgtcc atcgaagatc ctgtcgaaat caagcaggac gacatgcttc agttgcagtt
    16741 gaacgaagca atcggcctaa cctatgaaaa tctaatcaaa ctttccttgc gtcatcgacc
    16801 agatctcttg attatcggag aaattcgtga cagcgagacg gcgcgtgcag tggtcagagc
    16861 tagtttgaca ggtgcgacag tcttttcaac cattcacgcc aaaagtatcc gaggtgttta
    16921 tgagcgtctg ctggagttgg gtgtgagtga agaagaattg gcagttgttc tgcaaggagt
    16981 ctgctaccag agattaatcg ggggaggagg aatcgttgac tttgcaagca gagattatca
    17041 agaacaccaa gcagccaagt ggaatgagca aattgaccag cttcttaaag atggacatat
    17101 cacaagtctt caggctgaga cggaaaaaat tagctacagc taagcaaaaa aatatcatca
    17161 ccctatttaa caatctcttt tctagcggtt ttcatctggt ggagaccatc tcctttttag
    17221 ataggagtgc tttgttggac aagcagtgtg tgacccagat gcgtgtgggc ttgtctcagg
    17281 ggaaatcatt ctcagaaatg atggaaagtt tgggatgttc aagtaccatt gtcactcagt
    17341 tatctctagc tgaagttcat ggcaatcttc acctgagttt gggaaagata gaagaatatc
    17401 tagacaatct ggccaaggtc aagaaaaagt taattgaagt agcgacctat cctttgattt
    17461 tgctgggttt tcttctctta attatgctgg ggctacggaa ttacctgctc ccacaactgg
    17521 atagtagcaa tattgccacc cgaattatcg gtaatctgcc acaaattttt ctaggcatgg
    17581 tagggcttgt ttccgtgctt gcccttttag cactaacttt ttataaaaga agttctaaga
    17641 tgagtgtctt ttctatctta gcacgccttc cctttattgg aatctttgtg cagacctact
    17701 tgacagccta ttatgcacgt gaatggggga atatgatttc acagggaatg gagctgacgc
    17761 agatttttca aatgatgcag gaacaaggtt cccagctctt taaagaaatc ggtcaagatc
    17821 tggctcaaac cctgaaaaat ggccgtgaat tttctcagac gataggaacc tatcctttct
    17881 ttaggaagga attgagtctc atcatagagt atggggaagt taagtccaag ctgggtagtg
    17941 agttggaaat ctatgctgaa aaaacttggg aagccttttt tacccgagtc aaccgcacca
    18001 tgaatttggt gcagccactg gtttttatct ttgtggcact gattatcgtt ttactttatg
    18061 cggcaatgct catgcccatg tatcaaaata tggaggtaaa tttttaaaat gaaaaaaatg
    18121 atgacattct tgaaaaaagc taaggttaaa gcttttacat tggtggagat gttggtggtc
    18181 ttgctgatta tcagcgtgct tttcttgctc tttgtaccta atctgaccaa gcaaaaagaa
    18241 gcagtcaatg acaaaggaaa agcagctgtt gttaaggtgg tggaaagcca ggcagaactt
    18301 tatagcttgg aaaagaatga agatgctagc ctaagcaagt taaaagacga tgggcgaatc
    18361 acggaagaac aggttaaagc ttataaagaa tacaatgata aaaatggagg agcaaatcgt
    18421 aaagtcaatg attaaggcct ttaccatgct ggaaagtctc ttggttttgg gtcttgtgag
    18481 tatccttgcc ttgggcttgt ccggctctgt tcagtccact tttgcggcag tagaggaaca
    18541 gattttcttt atggaatttg aagaactcta tcgggaaacc caaaaacgca gtgtagccag
    18601 tcagcaaaag actagtctga acttagatgg gcagatgatt agcaatggca gtcaaaagtt
    18661 gccagtccct aaaggaattc aggccccatc aggccaaagt attatatttg accgtgctgg
    18721 gggcaattcg tccctggcta aggttgaatt tcagaccagt aaaggagcga ttcgctatca
    18781 attatatcta ggaaatggaa aaattaaacg cattaaggaa acaaaaaatt agggcagtga
    18841 ttttactgga agcagtagtc gctctagcta tctttgccag cattgcgacc ctccttttgg
    18901 gacaaattca aaaaaatagg caagaggaag caaaaatctt gcaaaaggaa gaagtcttga
    18961 gggtagctaa gatggccttg cagacggggc aaaatcagat aaacatcaac ggagttgaga
    19021 ttcaggtatt ttctagtgaa aaaggattgg aggtctacca tggttcagaa cagttgttgg
    19081 caatcaaaga gccataaggt caaggctttt accttgttag aatccctgct tgccctgctt
    19141 gtcatcagtg ggggcttact cctttttcaa gctatgagtc agctcctcat ttcagaagtt
    19201 cgctaccagc aacaaagcga gcaaaaggag tggctcttgt ttgtggacca acttgaggca
    19261 gaattagacc gttcgcagtt cgaaaaagta gaaggcaatc gcctatacat gaagcaagat
    19321 ggcaaggaca tcgccatcgg taagtcaaag tcagacgatt ttcgtaaaac cgatgctagt
    19381 ggacgaggtt atcagcctat ggtttatggg ctcaaatccg cacagattac agaggacaat
    19441 caactggtgc gctttcgttt ccagtttcaa aaaggcttag aaagggagtt catctatcgt
    19501 gtggaaaaag aaaaaagtta aggcaggtgt tctcctctac gcagtcacca tagcagccat
    19561 ctttagtctt ttgttgcaat tttatttgaa ccgacaaatc gcccactatc aagactatgc
    19621 tttaaataaa gaaaaattgg ttgcttttgc catggctaaa cgaaccaagg ataaagccga
    19681 gcaagaaagt ggggaacagg tctttaatct aggtcaggta agctatcaaa acaagaaaac
    19741 aagcttggtg acgactgttc gtacgtctaa gagccaatat gagtttctgt ttccttcagt
    19801 caaaatcaaa gaagagaaaa gagataaaaa ggaagaggta gcgaccgatt caagcgaaaa
    19861 agtggagaag aaaaaatcag aagagaagcc tgaaaagaaa gagaattcct agtcaattca
    19921 actataatgc gttgaatcca gaatagttca ctgtagtttc tagaaaattg ctggaaatgg
    19981 atgttaagct ccaatttatt tgtttatatc ttatttcagt ttactatact ttgtgctaaa
    20041 ctaaagatat gaaacatgat tttaaccaca aagcagaaac tttcgattcc cctaaaaata
    20101 tcttcctcgc aaacttggta tgtcaagcag tcgagaaaca gattgatatt ttatcagaca
    20161 aagaaatttt agatttcggt ggtggcacgg gtctattagc cttgccccta gccaagcagg
    20221 ctaagtcagt tactcttgta gacatttctg agaaaatgtt ggagcaagct catttgaaag
    20281 cggagcagca agcaatcaag aatatccagt ttttggagca agatttaccg aaaaatccct
    20341 tggagaaaga gtttgattgc cttgctgtta gtcgggttct tcatcatatg cctgatttgg
    20401 atgcggctct ctcactgttt catcaacatt tgaaggaaga tgggaaactc atcattgctg
    20461 attttaccaa gacagaagct aatcatcatg gatttgattt agctgaactg gaaaacaagc
    20521 taattgagca tggtttttca tctgtgcata gtcagattct ctatagcgct gaagacttgt
    20581 ttcaaggaaa tcactcagaa ttctttttaa tagtagccca aaaatcactc gcctagtcag
    20641 ggagtgattt ttctataagg atggaaaaaa gaagggaaat ttgataagat aggaatatgg
    20701 attttgaaaa aattgaacaa gcttatacct atttactaga gaatgtccaa gtcatccaaa
    20761 gtgatttggc gaccaacttt tatgacgcct tggtggagca aaacagcatc tatctggatg
    20821 gtgaaactga gctaaaccag gtcaaagaca acaatcaggc ccttaagcgt ttagcactac
    20881 gcaaagaaga atggctcaag acctaccagt ttctcttgat gaaggctggg caaacagaac
    20941 ccttgcaggc caatcaccag tttacaccgg atgctattgc tttgcttttg gtgtttattg
    21001 tggaagagtt gtttaaagag gaggaaatta ctatcctcga aatgggttct gggatgggaa
    21061 ttctaggcgc tactttcttg acctcgcttg ataaaaaggt ggattacttg ggaatggaag
    21121 tggatgattt actgattgat ctggcagcta gcatggcaga tgtaattggt ttgcaggctg
    21181 gctttgtcca aggagatgcc gttcgtccac aaatgctcaa agaaagcgat gtggtcatca
    21241 gtgacttgcc tgtcggctat tatcctgatg atgccgttgc gtcgcgtcat caagttgctt
    21301 ctagccaaga acatacttac gcccatcact tgctcatgga acaaggattt aagtacctta
    21361 agtcagatgg atatgctatt tttctagctc cgagtgattt gttgaccagt cctcaaagtg
    21421 atttgttgaa agtctggttg aaagaggagg caagtctggt tgctatgatt agtctgcctg
    21481 aaaatctctt tgctaatgct aaacaatcta agactatttt tatcctacag aagaaaagtg
    21541 aaatagcagt ggagcttttt gtttatccac ttgctagctt gcaagatgca agtgttttaa
    21601 tgaaatttaa agaaaatttt caaaaatgga ctcaaggtac tgaaatataa aatagatttt
    21661 gttataatag ttgaaaacgc ttaaaaaggg gtatcatgtt atgacaaaaa caattgcaat
    21721 caatgcagga agttcaagct taaaatggca attatactta atgccagagg aaaaagtatt
    21781 ggcgaaaggc ttgattgaac gtatcggttt gaaagattca atttcaactg taaaatttga
    21841 cggccgttct gaacaacaaa ttttggatat cgaaaatcat acacaagccg ttaaaatttt
    21901 attggatgac ttgattcgtt tcgatattat caaggcttat gacgagatta caggtgttgg
    21961 ccaccgtgtt gtagcaggtg gagaatattt caaagaatca acagttgttg agggagatgt
    22021 tttagaaaaa gttgaagagt tgagtttgtt ggctcctcta cacaacccgg ccaatgcagc
    22081 aggtgttcgt gccttcaagg aattgttgcc agacattacc agtgtagttg tttttgatac
    22141 ttccttccac acaagtatgc cagagaaagc ttatcgctac cctctaccaa caaaatatta
    22201 cacagaaaac aaggttcgta aatacggtgc tcatggtaca agtcaccagt ttgtagcagg
    22261 agaagctgca aaactcttgg gacgtccatt agaagacttg aagttaatta cctgtcatat
    22321 tggtaacgga ggctcaatta cagctgtgaa agccggcaaa tctgtagaca cttctatggg
    22381 gttcactcct cttggtggta ttatgatggg aacgcgtaca ggggatattg atccagctat
    22441 cattccttat ttaatgcaat atacagagga ttttaacaca ccagaagata tcagtcgtgt
    22501 tcttaaccgt gaatcaggtc ttttgggagt ttctgctaat tctagcgata tgcgcgatat
    22561 agaagcagct gtagcagaag ggaatcacga ggctagcttg gcttatgaaa tgtatgttga
    22621 ccgtatccaa aaacatatcg gtcagtacct tgcagtgcta aatggaacag atgccattgt
    22681 tttcacagca ggtgtcggtg aaaatgcaga gaatttccgt cgtgatgtaa tctcagggat
    22741 ttcgtggttt ggttgtgatg ttgatgatga aaagaatgtc tttggcgtta caggagacat
    22801 ctcaacagag gcagctaaaa tccgtgtctt ggttattcca acagatgaag aattagtcat
    22861 tgcccgtgac gttgaacgct tgaaaaaata agtgaaacta aaaaaatatt caatacaagg
    22921 agttgggaaa gttatttttc cagcttcttt ttctgatgaa attgtccaaa accttgctat
    22981 gattggcttt tttgaaaaat atggtataat agtagtaatt taatagatgg agttgagttt
    23041 tgaagaaaaa ctttcgtgta aaaagagaga aagattttaa ggcgattttc aaggagggga
    23101 caagttttgc taatcgcaaa tttgtggtct accaattaga aaaccagaaa aaccattttc
    23161 gagtaggtct atcagttagc aaaaaactgg ggaatgccgt cactagaaat caaattaagc
    23221 gacggattcg gcatattatc cagaatgcaa aagggagtct ggtagaagat gtcgactttg
    23281 ttgtcattgc tcgaaaagga gtcgaaacct tgggatacgc agagatggag aaaaatctac
    23341 tccatgtatt aaaattatca aagatttacc gggaaggaaa tgggagtgaa aaagaaacta
    23401 aagttgacta gtttgctagg actgtctctg ttaatcatga cagcctgtgc gactaatggg
    23461 gtaactagcg atattacagc cgaatcggct gatttttgga gtaaattggt ttacttcttt
    23521 gcggaaatca ttcgcttttt atcgtttgat attagtatcg gagtggggat tattctcttt
    23581 acggtcttga ttcgtacagt cctcttgcca gtctttcagg tgcaaatggt ggcttctagg
    23641 aaaatgcagg aagctcagcc acgcattaag gcgcttcgag aacaatatcc aggtcgagat
    23701 atggaaagca gaaccaaact agagcaggaa atgcgtaaag tatttaaaga aatgggtgtc
    23761 agacagtcag actctctttg gccgattttg attcagatgc cggttatttt ggccctgttc
    23821 caagccctat caagagttga ctttttaaag acaggtcatt tcttatggat taaccttggt
    23881 agtgtggata caacccttgt tcttccgatt ttagcagcag tattcacctt tttaagtact
    23941 tggttgtcca acaaagcttt gtctgagcga aatggcgcta cgactgcgat gatgtatggg
    24001 attccagtct tgatttttat ctttgcagtt tatgcgccag gtggagtcgc cctatactgg
    24061 acagtgtcta atgcttatca agtcttgcaa acctatttct tgaataatcc attcaagatt
    24121 atcgcagagc gcgaggccgt agtacaggca caaaaagatt tggaaaatag aaaaagaaaa
    24181 gccaagaaaa aggctcagaa aacgaaataa ataaggagga atctggtaat ggtagtattt
    24241 acaggttcaa ctgttgaaga agcaatccag aaaggattga aagaattaga tattccaaga
    24301 atgaaggctc atatcaaagt catttctagg gagaaaaaag gctttcttgg tctatttggt
    24361 aaaaaaccag cccaagtgga tattgaagcg attagtgaaa cgactgttgt caaagcaaat
    24421 caacaggtag taaaaggcgt tccgaaaaaa atcaatgatt tgaacgagcc tgtgaagacg
    24481 gttagtgaag aaaccgttga ccttggtcat gtagttgatg ctattaaaaa aatagaggaa
    24541 gaaggtcaag gtatttctga tgaagtcaag gctgaaatct taaaacatga aagacatgcc
    24601 agcactatct tagaagaaac tggtcacatt gagattttaa atgaacttca aatcgaggaa
    24661 gcgatgaggg aagaagcagg cgctgatgac cttgaaactg agcaagacca agctgaaagt
    24721 caagaactag aagacttggg cttgaaagtt gaaacgaact ttgatattga acaagtagct
    24781 acggaagtaa tggcttatgt tcaaacgatt attgatgaca tggatgttga ggctacactt
    24841 tcaaatgatt ataaccgtcg tagcatcaat ttgcaaattg atactaacga accaggtcgt
    24901 attatcggct accatggtaa agtcttgaag gccttgcaac tgttggctca aaattatctt
    24961 tacaactgct attcaagaac cttctatatc actattaatg tcaatgatta tgttgaacac
    25021 cgtgcagaag tcttgcagac ctatgcgcaa aaattggcga ttcgtgtttt agaagaagga
    25081 cgcagtcatc aaacagatcc aatgtcgaat agtgaacgca agattatcca ccgtattatt
    25141 tcacgtatgg atggcgtgac cagttactct gaaggtgatg agccaaatcg ctatgtcgtt
    25201 gtagatacag aataagtaaa atcagggttg acctgatttt ttgctagcta gaggaggtta
    25261 aactgatgtt gaataagata agagactatc tagacttttc tggtttgcag taccgtagtc
    25321 ctgataaagc gggagcagag cgagagaaga tgctggcatt ccgtcacaaa ggacaagagg
    25381 cccgaaaggc ttttacagaa ctggccaaag cctttcaagc aagccatcca gaatggcaac
    25441 tccaacagac tagtcagtgg atgaatcagg cccagcgttt gagaccacat ttttgggttt
    25501 atctacagag agacggacaa gtgacagaac ctatgatggc cttacgtttg tatgggacat
    25561 ctactgactt tggaatttct ttggaagtca gtttcatcga acgtaagaag gatgagcaaa
    25621 ctttagagaa acaggccaag attttagaca ttccaaccgt taaaggaatt tattatctag
    25681 cctactctga tggccaaagt caacggtggg aggcgaacga agaaaagcgt cgtactttac
    25741 gcgagaaggt gagaagtcaa gaagttcgaa aagttttagt gaaggtagat gttcctatga
    25801 cagaaaattc gtctgaagaa gaaatcgtag aaggcttatt gaagtcttat tctaaaattc
    25861 ttccctatta tctagctacg agaaaataag ataatttgta aaacatcata aatcatacag
    25921 tccaagagtg aacagtccgc tgtgtaattc ttggtctttt tgtttgcgct ttcgcattat
    25981 ataataaact tacaaaaaca attcaaaagg agaacaatta tggaagtcat ttcaagtgtt
    26041 ctaaattggt tttctagcaa tattttgcag aatcccgcat ttttcgtagg tttattggtg
    26101 ttgataggat atgcactttt gaaaaaacct gcccatgacg ttttttcagg gtttgttaaa
    26161 gcaacagtag ggtatatgtt gcttaacgtg ggtgctggtg gtttggttac aacctttcgt
    26221 ccaatcttag cagctcttaa ctacaaattc caaattggtg cagcggttat cgacccttac
    26281 tttggacttg ctgcagcaaa caacaaaatt gcagcagagt ttccagattt tgttggaact
    26341 gcaactacag ctctattgat tggttttgga ataaatatct tgctcgtagc tcttcgaaag
    26401 attacgaagg taagaaccct ctttattact ggtcacatca tggtacaaca agctgcaaca
    26461 gtatctctta tggttctatt cttagtacca caattgcgca atgcttacgg tacagcagcg
    26521 attggtatca tctgtggact ttactgggca gttagttcaa atatgactgt tgaggcaact
    26581 caacgcttga ctggtggtgg cggatttgcg attggtcacc aacagcaatt tgcaatctgg
    26641 tttgtagata aagtagcagg acgctttggt aagaaagaag aaagtttaga caatcttaaa
    26701 ttacctaagt tcctctcaat cttccacgat acagttgttg catctgctac cttgatgctc
    26761 gtattcttcg gagccattct tttaatcttg ggtccagaca ttatgtctaa taaagaagtc
    26821 atcacttcag gaactctatt caatcctgct aaacaagatt tctttatgta cattatccaa
    26881 acagccttta ccttctcagt ttacttgttc gttttgatgc aaggtgtccg aatgttcgta
    26941 tctgagttga caaacgcctt ccaaggtatt tcaaacaaat tgttgccagg ttcattccca
    27001 gcggttgacg ttgcagcttc ttatggattt ggttctccaa atgctgtcct gtcaggattt
    27061 atctttggtt tgattggtca attgattaca attgttttgc tcatcgtctt taaaaatccg
    27121 attcttatta ttacaggatt tgtaccagtg ttctttgaca atgcagccat tgcggtctac
    27181 gctgataaac gcggcggatg gaaagcggct gttatccttt cctttatatc aggtgtcctt
    27241 caagttgctc taggagctct ttgtgtggcc cttctcgatt tggcatctta tggtggctac
    27301 catggaaata tcgactttga attcccatgg cttggatttg gatatatctt caaatacctt
    27361 ggtattgttg gttatgtact tgtgtgtctc ttcttgcttg ttattcctca acttcaattt
    27421 gccaaagcaa aagataaaga gaaatattac aacggtgaag ttcaagaaga agcttagtat
    27481 ctagaaaagg agaaataaaa tggttaaagt attagcagcg tgcggaaatg gaatgggttc
    27541 atcaatggtt atcaagatga aggttgaaaa tgctctccgt aagcttaatc aaacagattt
    27601 tacagtcaat tcatgcagtg tcggtgaagc taaaggttta gcagtaggat atgacatcgt
    27661 aatcgcttct cttcatttga ttcaagaatt ggaagggcga actaatggga agttaattgg
    27721 gcttgataac ttgatggatg ataaagaaat caccgaaaaa ctcagtcaag cactacagta
    27781 aaaggttgga gggggctgga cagaaactga gagttatcgt ttctgtcctt ctccctcttt
    27841 aaataaagga ggcagatatg aatttaaaac aagctttaat tgacaacgac tcgatccgac
    27901 taggtttaga ggctaacgat tggaaagaag cagtcaaggt agcagtagat cccttgattg
    27961 aaagtggggc aattttgcca gagtattacg atgctatcat tgaatcgact gaagagtatg
    28021 ggccttacta tatcttgatg ccaggtatgg ctatgcccca cgctagacct gaagctggtg
    28081 tgcaaagtga tgccttttca ttaattacct tacaaaatcc tgttgtattt tcagatggga
    28141 aagaggtatc tgttttgttg gcactagcag caacaagttc aaaaattcac acaagtgtag
    28201 ccattccaca aattattgcc ctgtttgaat tagaagattc tattgcacgt ttacaggctt
    28261 gccagactaa agaagatgtc ttggctatga ttgaagaatc taaggatagc ccttatctcg
    28321 aaggattgga tttggaaagt tagaaagagg aataaagaaa tgacaaaaag aatacctaat
    28381 ttacaagttg cattagacca ttcagacttg caaggagcga ttaaagcagc tgtttctgtt
    28441 ggtcaggaag tagatattat cgaagctgga actgtttgct tgcttcaagt tggaagtgaa
    28501 ctggctgaag tcttgcgtag ccttttccca gataagatta ttgtggcaga cacaaaatgt
    28561 gctgatgctg gtggaacagt tgctaaaaat aatgcggttc gtggagcaga ctggatgact
    28621 tgtatctgtt gtgcaaccat ccctactatg gaagcagctc taaaggctat caagactgaa
    28681 cgaggagaac gaggcgaaat ccagatcgag ctttatggcg attggacttt tgaacaagct
    28741 cagctttggc tagatgcagg tatctcacaa gctatttatc accaatctcg tgatgctctt
    28801 cttgctggtg aaacttgggg tgaaaaagac cttaataagg ttaaaaaact cattgacatg
    28861 ggcttccgtg tatctgtaac aggtggtcta gatgtagata ctctcaaact ctttgaaggt
    28921 gttgatgtct ttacctttat cgcaggtcgt ggaattacag aggctgtgga tccagcagga
    28981 gcagcgcgtg ccttcaagga tgaaatcaaa cgaatttggg ggtaaatcat ggtacgtcca
    29041 attggaattt atgaaaaggc aaccccaaca cactttactt ggctagaacg tttaaatttt
    29101 gccaaggagt taggctttga ttttatcgag atgtctattg acgaacgtga cgagcgttta
    29161 gcaagacttg actggagtaa ggaagaacgc ttggaagttg tcaaagaaat ctatgaaact
    29221 ggtgttcgta ttccttctat ctgtttttca ggccatcgtc gctacccatt gggatcaaaa
    29281 gattcagttc tagaggaaaa atctctagaa ctcatgaaaa aatgtatcga attagctcaa
    29341 gacttgggag ttcgtacgat tcaattagct ggttacgatg tttactatga ggaaaagtca
    29401 ccccagacac gccaacgttt tatcaaaaat ttgagaaaag cctgtgactg ggctgaagaa
    29461 gctcaggtgg tacttgctat tgaaattatg gatgatcctt tcatcagtag catcgaaaaa
    29521 tatttggcta tagaaaaaga gattgactct cccttcctct ttgtatatcc agatattggt
    29581 aatgtgtctg catggcataa tgatatctat agtgagtttt atcttggtca tcatgccatc
    29641 gcagctctcc atctcaagga tacttatgca gtgacagaaa gttcaaaggg ccagttccga
    29701 gatgtacctt tcgggcaagg ttgtgtcaaa tgggaagaag ctttcgatat tttaaaggaa
    29761 accaattata atggaccttt cctaatcgaa atgtggtctg aaaattgtga aacagtagaa
    29821 gaaacacgcg cagccattca agaggcgcaa gcttttctct atccactcat taagaaagca
    29881 ggtttgatgt aagatgaatc aagtaatcaa tgctatgcgt aaacgagtct gtgatgccaa
    29941 tcaatcattg ccaaaacatg gacttgtcaa atttacctgg gggaatgtat ctgaagttaa
    30001 tcgcgaactc ggtgtcattg ttatcaaacc atcaggcgtg gattatgacg aattgacacc
    30061 tgaaaacatg gtagtgactg atctagatgg taagatccta gaaggggatt taagaccatc
    30121 ttccgacctc ccaactcatg tgcaattata taaggcttgg tcagaaattg gtagtgtggt
    30181 tcacacccat tcgacagaag ctgttggttg ggctcaggca ggtcgtgata ttcctttcta
    30241 cggaacaacc catgcagatt atttctacgg ttcaatccct tgcgcccgta gtttgaccaa
    30301 ggacgaagta gaagtggcct atgaaaaaga tactggcctg gttatcgtag aagagtttga
    30361 acatcgcgga cttaacccgg ttgaagtacc aggaattatt gtacgcaatc acggtccatt
    30421 cacctggggc aaaaatccag agaatgctgt ttatcactct gtcgtactag aggaagtatc
    30481 aaagatgaat cgctttacag aacaaatcaa tccaagagtt ggacctgctc cccagtacat
    30541 actagaaaaa cactaccaac gtaaacatgg accaaatgct tattatggtc aaaagtaaga
    30601 acgatgaagg aggagaaaaa gataaattta gctcctcttt ttacatttga tttttattga
    30661 gagtaaagtt ggaattgaag taattttaaa agatttttta gaaatagcgc ttgatatata
    30721 tggtaaaata aaaagaattg ctgtgatatc aatagatttg ggggattttt taatatggta
    30781 ctggataagg caagttgtga tttgcttcaa tatttgatgg atcaagaaac gtccaaaacg
    30841 attatggcga tttcgaaaga tttgaaagag tcaagaagga aaatttatta tcacattgac
    30901 aaaatcaatg ctgctctggg tgacgaggcg cttcacatca ttagtattcc acgaattggt
    30961 attcacttaa cggaagagca gagagatgct tgttgtaaac tattatcgga agtagattcg
    31021 tacgattata tcatgagtgc gcatgaacgt atgatgataa tgttactatg gataggtatt
    31081 tctaaagaac gtattacgat tgaaaaattg atagagttaa cagaggtatc taggaatact
    31141 gttctcaatg atttgaatag tattcgttat caactaactt tggaacaata tcaggtgatc
    31201 ttgcaagtga gcaagtcaca gggataccac cttcatgccc accctcttaa taaaattcag
    31261 tatcttcaat cgcttctata tcatattttt atggaagaaa atgccacttt tgtatctatt
    31321 ttagaagata agatgaaaga gaggttagat gatgagtgtt tgctttctgt tgaaatgaac
    31381 caatttttta aggaacaggt tcctttagtt gaacaagatt tagggaagaa aataaaccat
    31441 catgaaataa cttttatgtt gcaggttcta ccttatttgc tgttaagctg tcataatgtt
    31501 gaacagtatc aagaaagaca tcaggatata gagaaagaat tttctttgat aagaaaaaga
    31561 atagagtatc aggtgtctaa gaaattagga gaacggttgt ttcaaaagtt tgaaatttct
    31621 ttgtcaggac ttgaagtttc tcttgtagct gttctcctcc tctcctatcg taaagatttg
    31681 gatattcatg cagaaagtga tgattttcgg caattaaaac ttgctttaga agaatttatc
    31741 tggtattttg aatcacaaat ccgaatggag attgagaaca aggatgattt gttacgaaat
    31801 ttgatgatcc actgtaaagc cttgttattt agaaagactt acggtatttt ttctaaaaat
    31861 cctctaacaa aacaaattcg atccaagtat ggagaattat ttttagtcac tagaaaatct
    31921 gcggaaattt tagaaggagc atggtttatt cggctaacag acgatgatat tgcctatttg
    31981 acgattcata ttggaggatt tttaaaatat acaccatcgt ctcaaaaaaa tatgaaaaaa
    32041 gtttatctcg tttgtgatga aggtgttgcg gtttcgagac ttttgctgaa acaatgcaaa
    32101 ctttattttc caaatgagca aattgacact gtatttacaa cagaacaatt taagagtgtg
    32161 gaagatattg cacaagttga tgtagtgatt actactaatg atgatttgga tagcagattt
    32221 ccgattttaa gggttaatcc tatccttgaa gcagaagata ttttgaaaat gctagactat
    32281 cttaaacaca atatatttcg taataagagc aaaagtttca gtgaaaatct ttctagtctt
    32341 atttcgtctt atattgtaga cagcaagttg gctagtaagt tccaagaaga ggttcaaaca
    32401 cttataaatc aagaaatagt agttcaagct tttttggaag atatttgaag gacagtccaa
    32461 tgatgaacac aaacctgtgt ttttcttggt cttttttagt gttttgaagg gtggtatact
    32521 aatctcaaag ataacaatta tatccaaagg aggcaacata tgccaaacgt caaagaaatt
    32581 acaagagagt catggatttt agccactttc ccagagtggg gaacatggtt gaacgaagaa
    32641 atcgaagaag aagtcgtacc tgaaggcaac tttgccatgt ggtggctagg caactgtggt
    32701 acttggatta agacaccagc tggtgctaac gttgtcatgg acctttggtc aaaccgtgga
    32761 aaatcaacca aaaaagtgaa agatatggtt cgtgggcacc aaatggcaaa tatggcaggt
    32821 gttcgtaagt tgcaaccaaa cttgcgtgtt cagccaatgg ttatcgatcc atttgctatc
    32881 aacgaactag actattactt agtttcacac ttccacagtg atcatatcga cccatacaca
    32941 gctgcagcaa ttctcaacaa tcctaagtta gagcatgtta agtttatcgg tccttaccac
    33001 tgtggacgaa tctgggaagg atggggtgtt ccaaaagaac gtatcatcgt tgttaaacca
    33061 ggtgacacta tcgaattaaa agatatgaag attcatgcag tagaatcatt tgaccgtact
    33121 tgcttggtaa ctctcccagt gaacggtgct gatgagacag gcggtgaact tgctggcttg
    33181 gctgttacag atgaagaaat ggctcaaaag gctgttaact atatctttga aacaccaggt
    33241 ggaaccatct atcatggtgc agattctcac ttctcaaact attttgcaaa acatggtaaa
    33301 gactttaaaa ttgatgttgc tttgaataac tatggtgaaa atccggtagg tatccaagac
    33361 aaaatgacat ctatcgacct tcttcgtatg gcagaaaatc tgcgtaccaa agtcattatc
    33421 ccagttcact atgatatctg gtctaacttc atggcttcta ctaatgagat tctagaactt
    33481 tggaaaatgc gaaaagatcg cttgcaatac gatttccatc catttatctg ggaagttggc
    33541 ggtaagtaca cttatcctca agatcaacac ttagtagaat accatcatcc acgtggtttt
    33601 gatgattgtt ttgaacaaga ctctaacatt caatttaaag ctttgctata atataaaaat
    33661 attctttgga ggttatccgt atgttgcatg atacggataa ttttcatata atagtaaaat
    33721 agaatgtgtg attcaataat cacctcaaat agaaaggaaa ttctatgtca aatctatctg
    33781 ttaatgcaat tcgttttcta ggtattgacg ccattaataa agccaactca ggtcatccag
    33841 gtgtggttat gggagcggct ccgatggctt acagcctctt tacaaaacaa cttcatatca
    33901 atccagctca accaaactgg attaaccgcg accgctttat tctttcagca ggtcatggtt
    33961 caatgctcct ttatgctctt cttcaccttt ctggttttga agatgtcagc atggatgaga
    34021 ttaagagttt ccgtcaatgg ggttcaaaaa caccaggtca cccagaattt ggtcatacgg
    34081 cagggattga tgctacgaca ggtcctctag ggcaaggtat ttcaactgct actggttttg
    34141 cccaagcaga acgtttcttg gcagccaaat ataaccgtga aggctacaat atctttgacc
    34201 actatactta cgttatctgt ggagacggag acttgatgga aggtgtctca agcgaggcag
    34261 cttcatacgc aggtttgcaa aaacttgata agttggttgt tctttatgat tcaaatgata
    34321 tcaacttgga tggtgagaca aaggattcct ttacagaaag tgttcgtgac cgttacaatg
    34381 cctacggttg gcatactgcc ttggttgaaa atggaacaga cttggaagcc atccatgctg
    34441 ctatcgaaac agcaaaagct tcaggcaagc catctttgat tgaagtgaag acggttattg
    34501 gatacggttc tccaaacaaa caaggaacta atgctgtaca cggcgcccct cttggagcag
    34561 atgaaactgc atcaactcgt caagccctcg gttgggacta cgaaccattt gaaattccag
    34621 aacaagtata tgctgatttc aaagaacatg ttgcagaccg tggcgcatca gcttatcaag
    34681 cttggactaa attagttgca gattataaag aagctcatcc agaactggct gcagaagtag
    34741 aagccatcat cgacggacgt gatccagtcg aagtgactcc agcagacttc ccagctttag
    34801 aaaatggttt ttctcaagca actcgtaatt caagtcaaga tgccttgaat gttgtggcag
    34861 ctaaattacc aacctttcta ggtggatcag ctgaccttgc tcactcaaac atgacttata
    34921 tcaagacgga tggacttcaa gacgatgcca atcgcttgaa tcgtaacatt cagtttggtg
    34981 ttcgtgaatt tgcaatggga acaatcttga acgggatggc ccttcatggt ggacttcgtg
    35041 tatacggtgg aactttcttc gtcttctctg actatgtgaa ggcagctgtc cgcttgtcag
    35101 ccttacaagg acttcctgtg acttatgtct ttacccatga ttcaatcgca gttggggaag
    35161 atggtccgac tcacgaacca gttgagcatt tagcaggtct tcgtgctatg ccaaatctaa
    35221 atgttttccg tccagcagat gcgcgtgaaa cgcaagcagc ttggtacctt gcagtgacaa
    35281 gtgagaaaac accaactgcc cttgtcttga cacgtcaaaa cttgactgtt gaagatggaa
    35341 cagacttcga caaggttgct aaaggtgctt atgttgtata tgaaaatgca gccgactttg
    35401 ataccatctt gattgcgaca ggttcagagg ttaatcttgc tgtctcagct gctaaagaat
    35461 tggctagtca aggcgaaaaa atccgcgtag tcagcatgcc atctacagat gtctttgata
    35521 aacaagatgc agcttacaag gaagaaattc ttccaaatgc agtccgccgt cgtgttgcag
    35581 tcgaaatggg tgcaagtcaa aactggtaca aatatgttgg tctcgatggt gccgttctag
    35641 gtattgatac cttcggagcc tctgccccag caccaaaagt attggcagaa tatggcttta
    35701 ctgtagaaaa tcttgtaaaa attgttcgaa acttgaaata atcctaaaaa tcagggcgta
    35761 agctctggtt tttcttacca gaaaagtaag gtacaatctt gtaaaagtag ctgaaatttg
    35821 atatagtagt cctatgtaaa agacaaagga gaaaacaatg aatccaaata ttactttttt
    35881 aatcatgctt gtaggtatga tggccttgat gttctttatg caacgttctc aaaagaaaca
    35941 agctcaaaag cgtatggaaa gcttaaataa actacaaaaa ggctatgaag tgattacaat
    36001 cggtggactt tatggaacag tcgatgaagt agatacggag aagggaacaa tagttcttga
    36061 cgtagatgga gtttacttga cttttgaact agctgctatc aagacagtat taccgctgaa
    36121 agaaacagct tcactagaag gcgcaattga aaaataagac gggatcacga actcccgttt
    36181 ttctataaaa gaaaggaaat gggatgaaaa aattagtctt tgtctgtctg ggaaatattt
    36241 gccgtagccc tatggccgag tttgttatga aatcaatgac agataactac gaaatccaaa
    36301 gtcgagcaac ttcctcttgg gaacatggca atccgattca taaggggact cagggaattt
    36361 ttcaacagta tgagattctt tatgacaaga acaagacatc gcttcagatt agtaaggaag
    36421 attttgaagc ctttgattat attatcggaa tggacgcttc aaatatttct gacttacgtc
    36481 agatgtgtcc agtagactgt caagataaga tttactcatt ttcatctgaa agtgttccag
    36541 acccttggta tacaggagat tttgaagaaa cctatcgacg tgttcaagag ggctgtcaag
    36601 cttggttaga acgtttagaa aaggagagtt aaggtggaaa atcttacgaa tttttacgaa
    36661 aagtatcgtg tctatctgac tcgtccacgt ttagagcttt tggcagtagt taccattgtt
    36721 ttctgtgctg tactcgtctt ttttctaaat attccaggaa aaggtgtctt aaaactcgat
    36781 aatggaacga ttgtttatga tggcagtctt gtccgtggta aaatgaatgg tcaaggtacc
    36841 attaccttcc aaaatggaga ccaatataca ggtggcttca acaatggagc cttcaacgga
    36901 aaaggtacct ttcaatctaa agaaggctgg acctacgaag gtgattttgt aaatggtcag
    36961 gctgaaggaa aagggaaact aacaacagaa caagaagtcg tttatgaagg aacttttaaa
    37021 caaggcgttt ttcaacaaaa ataaagcctc cttatccaaa ggaggtatta ttagaaccac
    37081 aaggtaagcg cttacctgta aatccctttc tttccaaatc cctcttccaa gcaagtttgt
    37141 gaaataaaaa atatttgaaa taaatttcac aaacttcaaa gataaaacct gataagaaaa
    37201 gaaaatgaga aaagtttcac aagagtttaa aaattctttg tgaaatgttt atgaaactgt
    37261 ttacaaagtt gaaataatgc gttataataa acttgtgaaa aaattaacaa aggatatcgt
    37321 tccttgaaag ctatggagga aaatatggct gataaaaaaa ctgtgacacc agaggaaaag
    37381 aaactcgttg ctgaaaaaca cgtagatgag ttggttcaaa aagctctagt tgcccttgaa
    37441 gaaatgcgta aattggatca agagcaagtt gactacatcg ttgctaaagc atcagtagca
    37501 gctttggatg cccacggaga attggcttta catgcctttg aagaaacagg acgtggtgta
    37561 tttgaagata aagcaactaa gaacttgttt gcctgtgaac acgtagtaaa caacatgcgt
    37621 cacactaaga cagttggcgt tatcgaagaa gacgatgtaa caggattgac tcttattgct
    37681 gaaccagttg gtgttgtttg tggtattact ccaacaacaa acccaacatc aacagcaatc
    37741 ttcaaatcat tgatttcatt gaagacacgt aacccaatcg tctttgcctt ccatccatca
    37801 gcacaagaat catctgctca tgcagctcgt atcgtccgcg atgcagctat cgcagctggt
    37861 gctcctgaaa actgtgtgca atggattact caaccatcta tggaagcaac aagtgccctt
    37921 atgaaccacg aaggtgttgc gacaatcctt gcaacaggtg gtaatgccat ggttaaggcg
    37981 gcttattcat gtggtaaacc agctcttggg gtaggtgccg gaaacgttcc agcttatgtt
    38041 gaaaaatcag caaacattcg tcaagcagca cacgatatcg tcatgtctaa atcatttgat
    38101 aacggtatgg tctgtgcatc tgaacaagca gttatcattg ataaagaaat ttacgatgaa
    38161 tttgtagcag agttcaaatc ttaccacact tactttgtaa acaaaaaaga aaaagctctt
    38221 cttgaagagt tctgcttcgg cgtcaaagca aacagcaaaa actgtgctgg tgcaaaattg
    38281 aacgctgaca tcgttggtaa accagcaact tggattgcag aacaagcagg atttacagtt
    38341 ccagaaggaa caaacattct tgctgcagaa tgtaaagaag ttggcgaaaa tgagccattg
    38401 actcgtgaaa aattgtcacc agttattgca gttttgaaat ctgaaagccg tgaagatggt
    38461 attactaagg ctcgtcaaat ggttgaattt aacggtcttg gacactcagc agctatccac
    38521 acagctgacg aagaattgac taaagaattt ggtaaagctg ttaaagctat tcgtgttatc
    38581 tgtaactcac cttctacttt tggtggtatt ggggacgttt acaatgcctt cttgccatca
    38641 ttgacacttg gatgtggttc ttacggacgc aactcagttg gggataacgt tagtgccatt
    38701 aacctcttga atatcaaaaa agtcggaaga cggagaaata acatgcaatg gatgaaactt
    38761 ccttcaaaaa catactttga acgtgattca attcaatacc ttcaaaaatg tcgtgacgtt
    38821 gaacgtgtca tgatcgttac tgaccatgcc atggtagagc ttggtttcct tgatcgtatc
    38881 atcgaacaac tggaccttcg tcgcaataag gttgtttacc aaatctttgc ggatgtagaa
    38941 ccggatccag atatcacaac tgtaaaccgt ggtactgaga ttatgcgtgc cttcaaacca
    39001 gataccatca tcgcactcgg tggtgggtct ccaatggatg ctgccaaagt aatgtggctc
    39061 ttctacgagc aaccagaagt ggacttccgt gaccttgtcc aaaaattcat ggatatccgt
    39121 aaacgtgcct tcaagttccc attgcttggt aagaagacta aattcatcgc gattccaact
    39181 acatctggta caggatctga agtaacacca tttgccgtta tctctgataa agcaaacaac
    39241 cgtaaatacc caatcgctga ctactcattg acaccaactg tggcaatcgt agatcctgct
    39301 ttggtattga cagttccagg atttgttgct gctgatactg gtatggacgt attgactcac
    39361 gcgacagaag catacgtatc acaaatggct agtgactaca ctgatggttt agcacttcaa
    39421 gccattaaat tggtctttga aaatctcgaa agctcagtta agaatgcaga cttccactca
    39481 cgtgagaaaa tgcataacgc ttcaacaatc gctggtatgg cctttgccaa tgccttccta
    39541 ggtatttctc actcaatggc ccataagatt ggtgcgcaat tccacacaat ccacggtcgt
    39601 acaaatgcca tcttgcttcc atacgttatc cgttacaacg gtacacgtcc agctaagaca
    39661 gcaacatggc ctaagtacaa ctactaccgt gcagatgaaa aataccaaga tatcgcacgc
    39721 atgcttggac ttccagcttc tactccagaa gaaggggttg aatcttacgc aaaagctgtc
    39781 tacgaactcg gtgaacgtat tgggatccaa atgaatttta gagaccaagg aattgacgaa
    39841 aaagaatgga aagaacattc tcgtgaatta gccttcctgg cttatgaaga ccaatgttca
    39901 ccagctaacc cacgtcttcc aatggtagac catatgcaag aaatcatcga agatgcatac
    39961 tatggctaca aagaaagacc aggacgccgt aaataattgt ttatcagtct agaagcaaga
    40021 caaaaactca atttgaggga aagatccagt aatttttcta tgataaaagg catcctatca
    40081 aggtttttga acacctgata ggatgccttt ttatgatatt gaggcctttt tgcccttttt
    40141 gaaaaactag aatagaaaca aaatatataa tagattgaaa ctagaatagt acacatctgc
    40201 ttctaaaaca ttgttagaat tcgatttgac tgtcctgatc gatttgtcct gttcttattt
    40261 cattttgata tataaaaaat at
//
LOCUS       DAVKMU010000016        39918 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKMU010000016 DAVKMU010000000
VERSION     DAVKMU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.016"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            343..1386
                     /locus_tag="VW343_000939"
     CDS             343..1386
                     /locus_tag="VW343_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9755510.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIIARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1553..3640
                     /gene="topA"
                     /locus_tag="VW343_000940"
     CDS             1553..3640
                     /gene="topA"
                     /locus_tag="VW343_000940"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9755511.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEENIK"
     gene            3746..4105
                     /locus_tag="VW343_000941"
     CDS             3746..4105
                     /locus_tag="VW343_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9755512.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4198..4830
                     /locus_tag="VW343_000942"
     CDS             4198..4830
                     /locus_tag="VW343_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9755513.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGVLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQVGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            4874..5530
                     /locus_tag="VW343_000943"
     CDS             4874..5530
                     /locus_tag="VW343_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9755514.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5549..6169
                     /locus_tag="VW343_000944"
                     /pseudo
     CDS             5549..6169
                     /locus_tag="VW343_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            6197..6631
                     /locus_tag="VW343_000945"
     CDS             6197..6631
                     /locus_tag="VW343_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9755515.1"
                     /translation="MVAGSMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6643..7680
                     /gene="leuB"
                     /locus_tag="VW343_000946"
     CDS             6643..7680
                     /gene="leuB"
                     /locus_tag="VW343_000946"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9755516.1"
                     /translation="MAKKIVALVGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAWPPLPDETLKASREADAILLATIGSPQYDGAVVRPEQGLMTLRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSV
                     PDIAGQGIANPISMILSVSMMLRDSFGGYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7677..7946
                     /locus_tag="VW343_000947"
     CDS             7677..7946
                     /locus_tag="VW343_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9755517.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            7950..8309
                     /gene="leuD"
                     /locus_tag="VW343_000948"
     CDS             7950..8309
                     /gene="leuD"
                     /locus_tag="VW343_000948"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9755518.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8528..8671
                     /locus_tag="VW343_000949"
     CDS             8528..8671
                     /locus_tag="VW343_000949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755519.1"
                     /translation="MTSDKAGLERKFAAKEGKRNKPGVVLCGSMDELCALAQLNPKIE
                     AFY"
     gene            8690..8944
                     /locus_tag="VW343_000950"
     CDS             8690..8944
                     /locus_tag="VW343_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755520.1"
                     /translation="MLLGCILPWKPEAFEKLKAYGDGREELMTDVRGTSCFVIKFGKA
                     GEQLAAKLWEEGKMVYASYASMTKRLKLAMRARCNGVYGR"
     gene            8934..9095
                     /locus_tag="VW343_000951"
     CDS             8934..9095
                     /locus_tag="VW343_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755521.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNL
                     WDYRQVEYY"
     gene            9258..9621
                     /locus_tag="VW343_000952"
                     /pseudo
     CDS             9258..9621
                     /locus_tag="VW343_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828316.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            9630..9911
                     /locus_tag="VW343_000953"
     CDS             9630..9911
                     /locus_tag="VW343_000953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755522.1"
                     /translation="MYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTKLLCIYSEG
                     DSFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVPKLILSWSHRN"
     gene            9971..11188
                     /locus_tag="VW343_000954"
     CDS             9971..11188
                     /locus_tag="VW343_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9755523.1"
                     /translation="MDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTD
                     GNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGG
                     QDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYE
                     LYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEIS
                     KIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDRSVDTFD
                     FAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPS
                     YFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVA
                     DDNEGDENDDADY"
     gene            11175..12491
                     /locus_tag="VW343_000955"
     CDS             11175..12491
                     /locus_tag="VW343_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9755524.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            12666..13652
                     /locus_tag="VW343_000956"
     CDS             12666..13652
                     /locus_tag="VW343_000956"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9755525.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            13716..13787
                     /locus_tag="VW343_000957"
     tRNA            13716..13787
                     /locus_tag="VW343_000957"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13749..13751,aa:Arg,seq:ccg)
     gene            13970..14668
                     /locus_tag="VW343_000958"
     CDS             13970..14668
                     /locus_tag="VW343_000958"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9755526.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            14659..18198
                     /gene="smc"
                     /locus_tag="VW343_000959"
     CDS             14659..18198
                     /gene="smc"
                     /locus_tag="VW343_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9755527.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLEIILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEVIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18195..18989
                     /locus_tag="VW343_000960"
     CDS             18195..18989
                     /locus_tag="VW343_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9755528.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            18989..19807
                     /locus_tag="VW343_000961"
     CDS             18989..19807
                     /locus_tag="VW343_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9755529.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLQAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            19811..21100
                     /gene="ftsY"
                     /locus_tag="VW343_000962"
     CDS             19811..21100
                     /gene="ftsY"
                     /locus_tag="VW343_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9755530.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVTEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(21146..22633)
                     /locus_tag="VW343_000963"
     CDS             complement(21146..22633)
                     /locus_tag="VW343_000963"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9755531.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(22782..23522)
                     /locus_tag="VW343_000964"
     CDS             complement(22782..23522)
                     /locus_tag="VW343_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9755532.1"
                     /translation="MVKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(23522..25687)
                     /locus_tag="VW343_000965"
     CDS             complement(23522..25687)
                     /locus_tag="VW343_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9755533.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            25857..26810
                     /locus_tag="VW343_000966"
     CDS             25857..26810
                     /locus_tag="VW343_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9755534.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            26887..28875
                     /gene="uvrB"
                     /locus_tag="VW343_000967"
     CDS             26887..28875
                     /gene="uvrB"
                     /locus_tag="VW343_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9755535.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            28885..29448
                     /locus_tag="VW343_000968"
     CDS             28885..29448
                     /locus_tag="VW343_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755536.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            29414..29902
                     /locus_tag="VW343_000969"
     CDS             29414..29902
                     /locus_tag="VW343_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755537.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            29976..30444
                     /locus_tag="VW343_000970"
                     /pseudo
     CDS             29976..30444
                     /locus_tag="VW343_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
     gene            complement(30441..30956)
                     /locus_tag="VW343_000971"
     CDS             complement(30441..30956)
                     /locus_tag="VW343_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9755538.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(30968..31495)
                     /locus_tag="VW343_000972"
     CDS             complement(30968..31495)
                     /locus_tag="VW343_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9755539.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(31777..32340)
                     /locus_tag="VW343_000973"
     CDS             complement(31777..32340)
                     /locus_tag="VW343_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9755540.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(32324..32875)
                     /gene="coaC"
                     /locus_tag="VW343_000974"
     CDS             complement(32324..32875)
                     /gene="coaC"
                     /locus_tag="VW343_000974"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9755541.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(32887..33576)
                     /gene="coaB"
                     /locus_tag="VW343_000975"
     CDS             complement(32887..33576)
                     /gene="coaB"
                     /locus_tag="VW343_000975"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9755542.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            33806..35476
                     /locus_tag="VW343_000976"
     CDS             33806..35476
                     /locus_tag="VW343_000976"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9755543.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            35804..36979
                     /gene="mutY"
                     /locus_tag="VW343_000977"
     CDS             35804..36979
                     /gene="mutY"
                     /locus_tag="VW343_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9755544.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            37034..37738
                     /locus_tag="VW343_000978"
     CDS             37034..37738
                     /locus_tag="VW343_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9755545.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            37731..39080
                     /gene="vicK"
                     /locus_tag="VW343_000979"
     CDS             37731..39080
                     /gene="vicK"
                     /locus_tag="VW343_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9755546.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            39082..39891
                     /locus_tag="VW343_000980"
     CDS             39082..39891
                     /locus_tag="VW343_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9755547.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
BASE COUNT        12261 a         7097 c         8906 g        11654 t
ORIGIN      
        1 gtagagatta caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa
       61 tggttattat aaaaaatggt ctgaaataga tgatgatact tttcgaaaat ctttttacat
      121 cagctcagct ttgccttgct gtgttttgag caagctacgg ttagcttccg agtttgattt
      181 tcatttacta gaaatgaaac tgatgagaga tatcagtaga catctgagtc aggatattat
      241 ggaaaatgat aaaaagagct cgtgagattg gcatatcaga ctactaaagt attgagtttg
      301 ttaggatttt agcgactagt tagctgggaa aggaagatat ttgtgacaaa taataaactg
      361 tattcgttga tagaatttag aaataaaata tatgaagaat tagaactttc cagaagtgat
      421 ttagcgattt tactatgtgc catgcttatc gcctctatcg gattaaatat ggattcgact
      481 cccgtgatta ttggagccat gttaatctct cctttgatga cacctattct gggagtgggg
      541 ctctctctag ctatatttga ttttaaattg ttaagaaaat cttttaaaat attagctatt
      601 caaattcttg ccagtctaat agcttcaaca ctttattttt atctttctcc catttcgtat
      661 gctagttcgg agattattgc tagaacctct ccgactattt gggatgttct cattgctttt
      721 gtaggaggga tagcaggtat tattggtgct aggaaaaaag agaccaataa tattgttcct
      781 ggtgttgcta ttgcaaccgc cttgatgcct cctctttgta cagtaggtta tgctattgct
      841 tctgctaatc taaaatttat cataggctcc tcttacctat tcctcatcaa ttgtagcttt
      901 attgtcattg cgacttatat aggtgttagg ttgatgatgg ttaagaaaca ttattttaaa
      961 gataatgaag aagactctaa aatgcgtagg attttgcttc tagttgctgt tttgctgatg
     1021 attccgagtt tcatctctgc aacgacttta gtgagagaaa cgttgaaaaa agagtccctt
     1081 aagaaattta tatcagagca gtttcagggg cataatattt tgaaaaaaac ctattctaaa
     1141 aagactcata ccctaaagct aaccatttca ggaaattatt tgacagaaga agaactcgat
     1201 atgatttcaa gtaagagagg tgactatggt ttaagtgatg tttctgttca agtttcacaa
     1261 ttgtctgatt cagaacaact tagtaaggaa gaactggtgg agtatttctt ccagtatatc
     1321 aaggataagg aagcaaaaga aaaggaaaaa gctaataagt tttatacaga gtctgaggag
     1381 caataatttc ttgagaatag ctggtttttc tcgtgagtct tctatgtata tcaaaggaag
     1441 actgaggttt taagtataaa acttttcttc tattatagta gctaagtctt gacagggttc
     1501 aaaaaatggt ttacacttta taaagtttat tactttgaaa aggtgtgata ctgtggctac
     1561 ggcaacaaaa aagaaaaaat caacagttaa aaaaaatcta gtcatcgtgg agtcgcctgc
     1621 taaggccaag acgattgaaa aatatctagg cagaaactac aaggttttag ccagtgtcgg
     1681 gcatatccgt gatttgaaga aatccagtat gtccgttgat attgaaaata attatgaacc
     1741 gcaatatatc aatatccgag gaaaaggccc tcttatcaat gacttgaaaa aagaagctaa
     1801 aaaagctaat aaagtttttc tcgcgagtga cccggaccgt gaaggagaag cgatttcttg
     1861 gcatttggcc catattctca acttggatga aaatgatgcc aaccgtgtgg tcttcaatga
     1921 aatcaccaag gatgcagtca aaaatgcttt taaagaacct cgtaagatcg atatggactt
     1981 ggtcgatgcc caacaagctc gtcggatctt ggatcgcttg gtagggtatt cgatttcgcc
     2041 tattttgtgg aagaaggtca agaagggctt gtcagcaggt cgcgttcagt ccattgccct
     2101 taaactcatc attgaccgtg aaaatgaaat caatgccttc cagccagaag aatactggac
     2161 agttgatgct gtctttaaaa agggaaccaa acaatttcat gcttccttct atggagtaga
     2221 tggtaaaaag atgaaactga ccagcaatga cgaagtcaag gaagtcttgt ctcgtctgac
     2281 tagtaaagac ttttcagtag atcaggtgga taagaaagag cgcaagcgca atgctccttt
     2341 accctatacc acttcatcta tgcagatgga tgctgccaat aaaatcaatt tccgtactcg
     2401 aaaaaccatg atggttgccc aacagctcta tgaaggaatt aatatcggtt ctggtgttca
     2461 aggtttgatt acctatatgc gtaccgattc gactcgtatc agtcctgtag cgcaaaatga
     2521 ggcggcaagc ttcattacgg atcgttttgg tagtaagtat tctaagcacg gtagcaaggt
     2581 caaaaacgca tcaggtgctc aggatgccca tgaggctatt cgtccgtcaa gtgtctttaa
     2641 tacaccagaa agcatcgcta agtatctgga caaggatcag ctcaagctat atacccttat
     2701 ctggaatcgt tttgtggcta gccagatgac agcggccgtt tttgatacca tggctgttaa
     2761 attgtctcaa aaaggggttc aatttgctgc caatggtagt caggttaagt ttgatggtta
     2821 tcttgccatt tataatgatt ctgacaagaa taagatgtta ccggacatgg ttgttggaga
     2881 tgtggtcaaa caggtcaata gcaaaccaga gcaacatttc acccaaccgc ctgcccgtta
     2941 ttctgaagca acactgatta aaaccttaga ggaaaatggg gttggacgtc catcaaccta
     3001 cgcgccaacc attgaaacca ttcagaaacg ttattatgtt cgcctggcag ccaaacgttt
     3061 tgaaccgaca gagttgggag aaattgtcaa taagctcatc gttgaatatt tcccagatat
     3121 cgtaaacgtg accttcacag ctgaaatgga aggtaaactg gatgatgtcg aagttggaaa
     3181 agagcagtgg cgacgggtca ttgatgcctt ttacaaacca ttctctaaag aagttgccaa
     3241 ggctgaagaa gaaatggaaa aaatccagat taaggatgaa ccagctggat ttgactgtga
     3301 agtgtgtggc agtccaatgg tcattaaact tggtcgtttt ggtaaattct acgcttgtag
     3361 caatttccca gattgccgtc atacccaagc aatcgtgaaa gagattggtg ttgagtgtcc
     3421 aagctgtcat cagggacaaa ttattgagcg aaaaaccaag cgtaatcgcc tcttctatgg
     3481 ttgcaatcgc tatccagaat gtgaatttac ctcttgggac aagcctgttg gtcgtgactg
     3541 tccaaaatgt ggcaacttcc tcatggagaa aaaagtccgt ggtggtggca agcaggttgt
     3601 ttgtagcaaa ggcgactacg aggaagaaaa tattaaataa gaggaggggc ccgaaagtaa
     3661 atttcaggct ccttttggtt agaacttgac aaaatacatc attttatgcg aaactagaag
     3721 aagattattt taactaggag aagttatgcg tcttatctat ctgataattg gttttttatc
     3781 actggccttg gctattgttg gggttgtttt acccttgttg ccgacaacgc ctttcctttt
     3841 gttgtctatt gcttgtttct ccagaagttc caagcgtttc gaagactggc tttatcatac
     3901 caagctctat caaacatatg tagctgattt tcgcgagact aagtctatta cgcgtgaacg
     3961 aaagaaaaaa atcatcgtct ctatctacgt cttgatggga atttctattt attttgcacc
     4021 tcttttacca gtcaaaatcg gtctgggtgc tttgaccatc tttatcacct attatctctt
     4081 caaggtcatt ccagacaaag aataattaaa acagtagtta tttcccttga taaaattgaa
     4141 agcatattca taacaatatg atataataaa tttgaagtaa tattcaagga gaatcaaatg
     4201 atttacgaat tttgtgctga aaatgtgacc ttgcttgaaa aagcgatgca ggctggagct
     4261 cgtcggattg aactctgtga taatctagca gttggtggga caacccccag ctatggagtg
     4321 actaaggcag cggttgaact ggcagctaac tacgatacaa ccatcatgac catgattcgg
     4381 ccacgtggtg gtgactttgt ctataatgac ctagaaattg ctatcatgct agaagacatt
     4441 cgtttgactg ctcaggctgg aagtcaaggg gttgtatttg gagttttaac tgctgataaa
     4501 aagttggata agcctaatct ggaaaagtta attgctgcat caaaaggaat ggaaattgtc
     4561 tttcacatgg cctttgatga actaagtgat gaagatcaac cggaagctat tgactggctc
     4621 agtcaagtcg gtgtcactcg tatcctaact cgtgctggtg tgtctggcga ctccttagaa
     4681 aaacgttttg ttcactatca cagaattttg gagtacgcta aaggtaaaat tgaaattcta
     4741 ccaggtgggg ggattgacct tgacaaccgt caaaccttta tcgaccaggt gggggtaaca
     4801 cagttgcatg gtactaaggt tgttttttaa aaaatagaaa ggaactgcta gctttgggta
     4861 gcagttttca cttatgtttg aaatttttaa atcctatcaa tttaatcaag aaaaggctca
     4921 tgattatggt tttatagaaa atagcgaagt ctggacatat agttgccaga ttttgcaagg
     4981 tgactttgtc atgactgtgt ccatcactgc tgataatgtg aactttcaag tctttgacca
     5041 agagactggc gacctctatc ctcacgttta tatggaaagc atgaggggaa gttttgtcgg
     5101 aaatgtccgt gaggcttgtc tggagattct ttaccagatt cggaaggctt gttttgatgt
     5161 gcaagatttt atctgtcatc agactaagcg tatcatgact caagttcagg aaaagtatgg
     5221 aaaccagttg gagtatctgt gggaaaaatc gcctgataca gctgtattgc gccatgaagg
     5281 caatcaaaag tggtatgccg tcttgatgaa aatctcttgg aataagctgg aaaagggcag
     5341 agaaggacaa gtggaagcag tcaacctcaa gcatgaccaa gtagctaatt tactttcaca
     5401 aaaggggatt tatccagcct ttcatatgag caagcgctac tggattagtg tgtcccttga
     5461 tgatacttta tcagatgaag aagtactgga attgatagaa aaaagttgga acttaacctc
     5521 taaaaaatga aatattttaa taattttcat gaactttcaa ttagctaaat attctttact
     5581 gaagaaattt tcagaaaata taggatttac cacactagag gaatatggtg catcttcaaa
     5641 tacctgattg agaatgtcaa gacggatcgt cagatcatct attcgcctca ctgtcatgat
     5701 gacctcggaa tggcagtggc aaatagcctt gctgctgtca agaatggtgc aggacgtgtt
     5761 gaagggacta tcaatggtat tagggagcga gctggaaatg ctgctttgga agaaattgca
     5821 gtggctctca atattcgcca agattactac caagtagaaa ccagtattgt cctaaatgag
     5881 accatcaata cgtcagaaat ggtttctcgc ttctctggta ttccagttcc taaaaacaaa
     5941 gccgtcgttg gtggcaatac cttctcccac gaatctggta ttcaccaaga tggagtcctt
     6001 aaaaatcctc tcacttatga gatcatcaca cctgaattgg ttggtgttaa gattccgctt
     6061 gggtcgccat gcttttgttg agaaactgag agaattggcc ctagatttta cagaagagga
     6121 tatcaaacca ctctttgcta agttcaaggc actggccgat aagaagtaag aaatcacaga
     6181 tgcagatatt cgagctttgg tagctggaag catggttgaa aatccagaag gcttccactt
     6241 tgatgattta caacttcaaa ctcatgcaga taatgacatt gaagcgctcg ttagcctagc
     6301 caatatggat ggtgagaaag tcgaatttaa tgcgacaggg caaggttccg ttgaagcaat
     6361 ctttaatgct atcgataagt tctttaacca atctgttcgt ttggtgtcct acactatcga
     6421 tgcggtaaca gatggaatcg atacccagga tcgggttttg gtcactgttg aaaacagaga
     6481 tacagaaacc atctttaatg cagcagggct tgattttgat gtgttgaagg cttctgctat
     6541 tgtctatata aacgctaata cctttgttca aaaagagaat gcaggtgaga tgggacgcag
     6601 tgtttcttac cacgatatgc ctagtgtgta aaggagaagg ctatggcaaa gaaaatagta
     6661 gctctagtag gagacggaat tggcccagaa atcatggagg ttggtttaga agttctggag
     6721 gctctagctg aaaaaacagg ttttgactat gagattgaca gacgaccgtt cggaggtgca
     6781 gatattgatg cagcatggcc tcccttacct gatgaaaccc ttaaggcaag tagggaagca
     6841 gatgctatcc tactagcaac tatcggtagt cctcagtatg atggagcagt ggttcgccct
     6901 gaacaaggcc tgatgactct ccgtaaggaa cttaatcttt acgctaatat tcgtcctgta
     6961 aaaatctttg acagtctcaa gcatttgtca ccactcaaac tggaacgaat tgctggtgta
     7021 gactttgtcg tggtgcgtga attgacaggc gggatttact ttggagatca tattcttgaa
     7081 gagcgcaatg cgcgtgatat caacgactat agctatgagg aagtggagcg gattattcgc
     7141 aaagcctttg aaattgcaag aaatcgcaga aaaatcctta ctagtatcga taagcaaaat
     7201 gttctagcga cctcaaaact ctggcggaaa gtagctgagg aagtcgcaca ggatttctca
     7261 gatgtaacct tggaacacca gctggtagac tcagctgcta tgcttatgat taccaatcct
     7321 gctaagtttg atgttattgt aacggagaat ctttttggag atattttatc tgatgaatca
     7381 agcgtcttat ctggtacact tggggttatg ccatcagcca gtcattctga aaatggacca
     7441 agtctctatg aacctattca cggttcagta cctgatattg caggtcaagg aattgccaat
     7501 cctatttcca tgattttatc agtttccatg atgttaagag atagtttcgg aggttatgag
     7561 gatgcagagc gtatcaaacg tgctgttgag acaagtttgg cggcaggaat tttaacgaga
     7621 gatataggag gtcaggcttc aacaaaggaa atgacggaag ctattattgc aaggttatga
     7681 agttagacga aaaaattact ctagtccttt tgatttggaa tgtcatcatt ttcttgattt
     7741 atggcattga caaattcaag gcaagaagaa gagcttggcg catcccagag aaaatcttac
     7801 tcattttagc ccttacttgt ggtggttttg gggcctggtt agctggaatc acttttcacc
     7861 acaagactcg aaaatggtac tttaaaacag tttggtttct tgggatggtg accacactag
     7921 tagccttata ttttatttgg aggtaatgga tggcagggtc ttcgagggaa cacgctgctt
     7981 gggctctagc ggactatggt tttaaggtcg tgattgcagg atctttcggt gacattcatt
     8041 acaataatga actcaataat ggcatgttgc caatcgttca gcctagagag gttagagaga
     8101 aactagccca gctaaaacca accgaccagg taactgtgga cttggaacaa caaaaaatca
     8161 tctcaccagt tgaagaattc accttcgaga tagatagcga gtggaaacat aaactcctaa
     8221 atagtttgga tgatatcggt attaccttgc agtatgaaga gttgattgct gcttatgaaa
     8281 aacaacgacc agcctactgg caggattaga aaaaatagaa aaggaaatat agtaaactga
     8341 aataagatgt aaacaaatga attggagctt aacatccatt tccagcaatt ttttagaaac
     8401 tacagtggac tattctggat tcaacacatt ataaaattat gacaaaacac attcacaaga
     8461 aggctacgac attttaaaag gtgagggcgg atgtatcgtt tgccctacta aagttggtta
     8521 cattatcatg accagcgaca aggcaggact tgagcgtaag tttgcagcca aagaaggtaa
     8581 gcgtaacaaa ccaggtgttg ttctctgcgg tagcatggat gaactttgcg ctttagcgca
     8641 actcaaccca aaaattgaag cattctacta aaaacattgg gatgaagata ttcttcttgg
     8701 ttgtatcctt ccttggaaac cagaagcctt tgaaaaactc aaagcatacg gggatggccg
     8761 tgaagaactt atgactgatg tacgtggtac tagctgtttt gttatcaagt ttggaaaagc
     8821 aggtgaacaa ttggctgcca agctttggga agaaggtaaa atggtctacg cctcatatgc
     8881 ttcaatgaca aaacgattga aactcgctat gagagcaagg tgtaatggtg tctatggtcg
     8941 ataaggacgg caaactcatc ccagaacaag gaggagcacg ttcaacctca ccagcaccag
     9001 ttgtgatccg taaagggctt gacattgata aaatcatgat gcacctgtca gacaccttta
     9061 acttatggga ctaccgtcag gttgagtatt attaggatag agaagaagtc tagtgttaag
     9121 gacattacag ctcataatac taggcttttt tatagaaatt cttttctttt tctataggat
     9181 atggtattct atgtagaaaa tatatgttaa taagtaatgc caatatttaa acatcattag
     9241 taaaaggagt tagattgatg aataaaagaa aagttagttt agaagatttt tataaatggt
     9301 atagtctaaa taaagaagag ttattaaata aggcaactgt tggtgaaaag tttaatgata
     9361 aattaaaaga agagtttctc caggaatggc ctttggatag gattttaaca atgtcaatcg
     9421 atgaatatgt aataggaaag ggacagcaaa ataagtcttt atgctacgct cttgagaagg
     9481 gaaaatacaa aaatctattt cttggaattt ctggtggctc agcttcaaaa tttggtattt
     9541 attggaataa aaaaacaaac aaatataaag atcaagctaa taatgagatt tcagagttgg
     9601 atcagcgatt ttcaaaatta aaatcagatt tgtatgaaat tatcaaagaa ggtattcgtt
     9661 ttaactttga aaatcctatt tttgatatga aaagatcaac aaatgaattt attggtcgtt
     9721 ctgctatggt gacaaaatta ctttgtatct attctgaggg agattctttc tttggtgtaa
     9781 atattaatag tcagaaagaa ttttggaacc actttgtttc tcagacaaat caaggtggac
     9841 cttatctgca aaatcataaa ataattgaac tggtgccaaa acttatcctg agttggagcc
     9901 atcgaaatta ggaactatgc tttttgagta ttctaagctt tttatggaaa ataaggaaga
     9961 caatagtaca atggattcat caaacaattt tcgtcatcaa ttaactcaat ctctattaaa
    10021 gtctccaaac cttatccttc gtggtgctcc tggcacagga aagacttatc ttgctaaaga
    10081 aattgccaaa gaattaacgg atggcaacga agaccaaatc ggatttgtac aatttcaccc
    10141 atcatatgat tatacggatt ttgtagaagg tttaagacca gtatcaaatg gggatggagc
    10201 tattgagttt aggctacagg acggtatttt taaagatttt tgtcagaaag caaaagaaac
    10261 ccaattgatt ggaggacaag ataattttga tgaggcttgg gattcttact tagaatatat
    10321 aaatgttgct gaagaaaaag aatatataac aaaaacatct tacttatctg ttaatagtag
    10381 acaaaatttg tcagtaaatt atgatagtgg tgttccagga tggtcactac ctagcaaata
    10441 tgtttacgag ttgtataaag ataaaaatta taataagcaa gaatactaca aaagtggtgg
    10501 aaaaactgtc ctagaaacat tgagaaagag atttggtttg aaagactatg tttccccaac
    10561 agaaattgat actgataaga aattcgtctt catcatcgat gaaatcaatc gtggggagat
    10621 ttctaagatt tttggcgaac tctttttctc tatcgaccct ggctatcgtg gtgaaaaagg
    10681 aagtgtttct acccaatatg caaatctaca cgaaactgat gaaaagttct atatccccga
    10741 aaatgtttac atcatcggaa ctatgaatga tattgatcgt tcagtggata cctttgattt
    10801 tgctatgcgt cgtcgttttc gttttgttga agttactgcc gagggtcaag ttggcatgtt
    10861 ggataaagag ctgaatatcc atgcagaaga agcaaaaatt cgtctaagaa acttgaacgc
    10921 tgctatcgaa aatgttcagg aattaaacag tcattatcat attggaccaa gttattttct
    10981 taagttgaag gatgtagatt ttgactatga attactctgg tccgattata ttaagcctct
    11041 cctagaagac tacttgcgag gttcttatga tgaggttgaa actttggaaa ctttgaaaaa
    11101 agcatttgaa ctgacaaata atgagcaaaa agatcaggca gtagctgatg acaatgaagg
    11161 tgatgaaaac gatgatgcgg attactgata atcaacacaa gattattaaa gaaaaatttg
    11221 ttgaagaata tcctaaacta agcaatcttc ttttagacag aaccttggaa agtctatccc
    11281 aagatgaacg tattttcatt tttccaaatg atttgactca tactcctgat ttggataagg
    11341 accaaaagat ttttgaaaca gtcaatcaga aaatcaagac agggaacgtg attggttttc
    11401 ttggatatgg tcaggaaaga ttaacgattt cctcacgatt ttctgatgag agtaatgacc
    11461 attttttgca ttatctctta aacaaggttc ttcatatcaa tctcactagt ttagatgttg
    11521 ctttgtctcg tgaagagagg ctttatcaac ttttggtgta tctctttccc aagtatctac
    11581 aagctgctat tcgaaaaggt ctttataagg aatatcatcg attttctcat aacgacagta
    11641 atgttaaggg agtgattgat gtaagaagcc atctcaagaa aaatcttcct ttcacgggaa
    11701 atattgccta cgcaacgaga gagttcacct atgataatcc cctcatgcag ttggtccgtc
    11761 acactattga atacattaag aatcagaaaa gcattggtca aggggtacta gataatctct
    11821 caactagtcg tgaaaacgta tctgaaatcg tgcgtgtaac gccctcttat aaactagctg
    11881 atcgtgctaa gattattcgg ggaaatcaat ctaaacctat acgtcatgca tactttcacg
    11941 agtacagaaa cttacaagaa ctttgtctga tgatcctaaa ccaagaaaag cacggtttag
    12001 ggtatcaaga tcaaaaaatc tatggtattc tctttgatgt tgcctggctt tgggaagagt
    12061 atgtttacac cttgttgcca aaaggttttg tacatcccag aaataaggat aagacggatg
    12121 gaatttcagt attttctgtt gggaaacgaa aagtatatcc agatttttat gacagagaac
    12181 gaaagattgt tctagatgca aaatataaaa aactggaatt gactgaaaaa ggaatcaacc
    12241 gtgaggactt attccagctg atttcctatt cttatatttt aaaagctgag aaggctggac
    12301 tgatttttcc tagtatggag cagtcagtaa atagtgaaat aggaaaagta gctggctatg
    12361 gagctcaatt gaagaagtgg tctattcgaa tccctcagaa tgcctcattc tatagtacat
    12421 tttgtaaaat gatggaaaat tcagaagaaa attttaaagc gattattgat gaagaagtgg
    12481 ggagaaaata atttcccctc tttcttttat ttttaacaca agtaaaaccc gaacgatata
    12541 ttttaattca taaaataatt tgacaaaaac tgtactttgg tttataataa attaaggaaa
    12601 cgtcggaaaa ggcgtagtga tgcaagagca taggtaggtc attacaaaag aaacgagaca
    12661 tcgatatgtt aaatgaattt ccaatttttg attacgaaga tattcaattg attccaaata
    12721 aatgtgtcat taaaagccgt gcagaagcgg atacaagtgt cactttagga aatcacacct
    12781 ttaaactacc tgttgtgcca gcgaatatgc agacgatttt ggatgaaaat gtagcagagc
    12841 aactggctaa aggtggttac ttctacatta tgcaccgttt tgatgaggca ggacgcattc
    12901 cttttattaa acgaatgcac gatcaagggc tcattgcttc tatctctgtc ggtgttaagg
    12961 attatgagta tgatttcgtt agacagctca aggctgatgc tcctgagtat atcactattg
    13021 atattgctca tggtcatgcg gatagcgtga tttctatgat tcaacacatc aagaaagaat
    13081 tgccagatac ctttgtcatt gctggaaatg tgggaacacc agaagctgtg cgtgaattgg
    13141 aaaatgctgg tgcggatgct actaaggtcg gaatcggtcc tggtaaggtt tgtatcacca
    13201 aggttaagac tggttttggt acaggtggtt ggcagttggc tgctctacgc tggtgtgcca
    13261 aggctgcgcg taaaccgatt atcgctgatg gaggaattcg tactcacggt gatattgcta
    13321 agtctatccg cttcggtgct agcatgatca tgattggttc cctctttgca ggacacattg
    13381 aaagtccagg gaaaacgatt gaagtcgatg gtgaacagtt caaagaatat tatggttcag
    13441 cctcacaata tcaaaaaggt gcttacaaaa atgtggaagg caaacgtatc ttgcttcctg
    13501 ctaaaggtca tctgcaagac actttaactg agatggaaca agaccttcaa agtgctattt
    13561 cttatgcagg tggacgtcag gttgctgacc ttaaacacgt tgattatgtg atcgtgaaaa
    13621 actccatctg gaatggggat gcttcccact aagacggggt ctttgaccaa aaaacttgtt
    13681 attagagcaa atttctgtta taataaaaca agtttccacc cttagtgtaa tggatatcac
    13741 gtaagattcc ggttcttgag atgggggttc gattccctca gggtggatgt aaacatccta
    13801 aaaagccttt aatagggctt ttttctttat cctgacccaa atttgccgct aaaactgaga
    13861 atctctgtct aaagaattcc caaatgcttt ccagtcccgt tttagagtga ctcagggggc
    13921 tttttttgat ataataaaaa ggactattat cagttagaaa gaggttggta tgaaagaatt
    13981 acaaactgta ctaaagaatc attttgcaat cgaatttgca gacaaaaatt tactggaaac
    14041 tgcctttact catacgagtt atgccaatga gcaccgcctc ttaaaaattt cacacaatga
    14101 acgcttggaa tttttaggag acgctgttct acagttattg atttcagaat atctatataa
    14161 aaaatatcct aaaaagcctg aaggtgacct atcaaaactc cgtgctatga ttgtccgtga
    14221 ggagagttta gctggttttg cgcgtgattg ccagtttaac cagtttatca agttgggtaa
    14281 aggggaagaa aaatcaggtg gtcgcaatcg tgacaccatt cttggtgatg cctttgaagc
    14341 ctttcttggt gcccttcttt tggataagga tgtggccaag gtcaaggaat ttatctatca
    14401 agtcatcatt cctaaggttg aagcaggcga gtttgagatg attacagact ataaaaccca
    14461 tctccaagag ttgcttcagg tcaatggtga tgtggctatt cgttatcagg tgatttctga
    14521 aacagggcct gctcatgata aggtctttga tgtagaagtt cttgttgaag gtaagagcat
    14581 cggtcaaggc caaggtcgtt ctaagaaatt agcagagcag gaagctgcca aaaatgccgt
    14641 tgagaagggg ctggattcat gtatttaaag gaaatcgaaa ttcaggggtt caagtctttt
    14701 gctgataaga ccaaggtcgt ttttgaccaa ggtgtgacgg cagttgttgg acccaatgga
    14761 tctggaaagt ccaatattac agaaagtctg cgttgggctt tgggggagtc tagtgtcaag
    14821 agtctccgtg ggggcaagat gccggatgtc atctttgctg gaaccgaaag tcgcaaaccg
    14881 ctcaattatg cttctgtagt tgtgactctg gataatcatg acggatttat caaggatgca
    14941 ggtcaagaaa tcagggtgga acgccatatc tatcgtagtg gagatagcga atacaagatt
    15001 gacggcaaga aagtccgtct gcgtgatatt catgacctct tcttggatac tggattggga
    15061 cgagattcct tctctattat ttcccaaggg aaggttgagg agatttttaa ttccaagcct
    15121 gaggaacgac gagctatttt tgaagaagct gctggagttt taaaatacaa gactcgcaga
    15181 aaagaaaccg agagtaaact gcagcaaact caggataatc tggaccgctt agaggacatt
    15241 atctacgagt tggataatca aatcaagcct cttgaaaagc aagctgagaa tgcccgtaag
    15301 tttttagact tggaaggaca acgtaaggct atttatttag atgttctggt tgctcaaatc
    15361 aaggaaaata aggctgaact agagtcgaca gaagaagagt tggctcaggt tcaagaactc
    15421 ttgatgagtt attaccaaaa gcgtgaaaaa ttagaagaag aaaatcaaac tcttaaaaag
    15481 caacgccaag atttacaggc tgaaatggcc aaagaccaag gcagtttgat ggatttgacc
    15541 agtctgatta gtgacttaga gagaaaatta gccctatcga aactggaatc tgagcaagta
    15601 gccctcaatc aacaggaagc acaagcccgt ttggctactt tggaggataa gagaagttca
    15661 ctcagccaag aaaaatctga taaagaaagc tctttagccc tcttggaaga aaatctagtc
    15721 caaaataatc aaaaactcaa tcgattagag gctgaattgc tagctttttc agatgatcct
    15781 gatcagatga ttgagctctt acgtgaacgc tttgtggccc ttttacaaga agaagcagat
    15841 gtctcaaacc aactgacccg tattgagaat gagttggaaa atagtcgtca gctttctcaa
    15901 aaacaagcag atcaactaga aaagctgaaa gaacagttgg ctacagctaa agagaaggct
    15961 ggtcagcaaa aggctgagct tgaaactgcc aaggagcagg ttcagaaatt attggccgac
    16021 tatcaagtct gtgctaagga gcaagaggag cagaaaactt cctatcaagc tcaacaaagt
    16081 caactctttg accgtctgga tagtctcaaa aacaagcagg ctagagcgca aagtttggaa
    16141 attatcctga gaaatcatag taacttttat gcaggtgtta agagtgttct ccaagagaaa
    16201 gatcgtctag gtgggattat tggagcagtt agtgagcacc tgacctttga tgtgcattat
    16261 caaactgccc tagagattgc gcttggagcg agtagccaac atatcatcgt ggaagatgaa
    16321 gagtcggcaa ccaaggcgat tgatttactc aaacgaaaca gatctggtcg tgcaaccttc
    16381 cttcctttga ccactatcaa ggctcgtacg atttctagtc agaaccaaga tactattgct
    16441 gcaagtccag gatttctggg catggcagat gagctggtta ctttcgatac tagactggaa
    16501 gccattttca agaatttgct agctacaacg gccatttttg ataccgtgga gcatgcgcgt
    16561 gcagcagcgc gccaggttcg ttatcaggtt cgtatggtga cattggacgg aacagagctg
    16621 cgcacaggtg gttcttatgc aggtggagct aatcgccaga ataacagtat tttcatcaag
    16681 ccagaactgg agcaattaca aaaagaaatt gctgaagaag aagcaagttt gcgttcagat
    16741 gaagtgagtt tgaagaactt gcaagatgag ctagctagat tgacagaaag attagaagcc
    16801 atcaaatctc agggagagca ggctcgtatt caggagcaag gcttgtccct cgcctatcag
    16861 cagaccagtc agcaagttga agagctagaa actctttgga aactccaaga agaggaatta
    16921 aatcgtcttt ctgagggaga atggcaagcg gataaggaaa aatgccaaga gcgccttact
    16981 acaatcacta gtgaaaagaa taatctggaa gctgagattg aagagattaa gtctaataaa
    17041 aacgccatcc aagaacgtta tcaaaacttg caggaagagc tagctcaagc tcgtctactt
    17101 aagacagaac tgcaagggca aaaacgttat gaagttgctg atattgaacg cttaggtaag
    17161 gaactagata atcttgatat tgagcaagaa gaaattcagc gccttcttca agaaaaggtt
    17221 gacaatcttg agaaagttga tacggatttg ctcagtcaac aggctgatga agccaaaact
    17281 caaaaaacaa atctccaaca aggtttgatt cgcaagcagt ttgagttgga tgatattgag
    17341 ggtcaactgg atgatattgc tagtcatttg gatcaggctc gccagcagaa tgaagagtgg
    17401 attcgcaagc aaactcgtgc tgaagccaag aaagaaaagg ttagcgagcg cttgcgccat
    17461 ctacaaagtc aattaacaga ccagtaccag atcagctaca cagaagcttt agaaaaggcg
    17521 catgagttgg aaaatctcaa tctggcagag caagaagtta aggatttaga gaaggctata
    17581 cgctcactgg gtcctgtcaa tttagacgct attgaacagt acgaagaagt tcacaaccgt
    17641 ctggatttcc taaatagcca acgtgatgat attttgtctg cgaaaaacct gctccttgag
    17701 accattacag agatgaatga tgaggtcaag gaacgcttta aatcaacctt tgaggtcatt
    17761 cgtgagtcct ttaaagtgac cttcaagcag atgtttggtg gaggtcaggc tgacctcatt
    17821 ttgactgaag gagacttgct gacagctggg gttgaaattt ctgtccaacc accaggcaag
    17881 aaaatccagt ctcttaatct catgagtggt ggtgaaaaag ccctatcggc tcttgccttg
    17941 ctcttctcca ttattcgtgt caagactatt ccatttgtta tcttggatga ggtggaagct
    18001 gcgctggatg aagccaatgt taaacgtttt ggggattacc tcaaccgctt tgacaaggac
    18061 agccagttca tcgtcgtaac ccaccgtaag ggaaccatgg cagcggctga ttccatctat
    18121 ggagtgacca tgcaagaatc gggtgtttca aagattgttt cagttaagtt aaaagattta
    18181 gaaagtattg aaggatgaca attaaactag tagcaacgga tatggacgga accttcctag
    18241 atgggaatgg acgctttgat atggatcgtc tcaagtctct cttggtttcc tacaaggaaa
    18301 aagggattta ctttgcggta gcttcgggtc ggggatttct gtctctagaa aaattatttg
    18361 ctggtgttcg tgatgacatt attttcatcg cggaaaatgg cagtttggta gagtatcaag
    18421 gtcaggactt gtatgaagcg actatgtctc gtgactttta tctggcaact tttgaaaagc
    18481 tgaaaacttc accttatgta gatatcaata aactgctctt gacgggtaag aagggttcat
    18541 atgttctaga tacggttgat gagacctatt tgaaagtgag tcagcactat aatgaaaata
    18601 tccaaaaagt agcgagtttg gaagatatca cagatgacat tttcaaattt acaaccaact
    18661 tcacagaaga aacgctggaa gatggggagg cttgggtaaa cgaaaacgtt cctggtgtta
    18721 aggccatgac aactggcttt gaatccattg atattgttct ggactatgtc gataagggag
    18781 tggccattgt tgaattagtt aaaaaacttg gtatcacaat ggatcaggtc atggcttttg
    18841 gagacaatct taatgactta catatgatgc aggttgtggg acatcctgta gctcctgaaa
    18901 atgcacgacc tgaaatttta gaattagcaa agactgtgat tggtcaccat aaggaacggt
    18961 cggttatagc ttatatggag ggcttataat ggcagatata aaattgattg cattggactt
    19021 ggacgggacc ttgctgacta ctgataaaag gctgacggat cgtaccaagg aaaccttgca
    19081 agctgcgcgt gatcgtggta tcaaggtcgt attgacaact ggtcgtccct taaaagccat
    19141 ggatttcttt ctccatgagt tagggactga cggtcaggaa gatgagtata ccattacttt
    19201 taatggtgga ttagttcaga aaaatacagg agaaatcctt gataaaacag tcttttcata
    19261 tgatgatgtg gcacgtttgt atgaagaaac agagaaatta tcactgcctc ttgatgccat
    19321 ctcagaagga acagtttatc aaatccaatc ggaccaagaa agtctttatg ccaaattcaa
    19381 tccagctttg acctttgttc cagtggactt tgaagactta tctagtcaaa tgacctacaa
    19441 caaatgcgtg actgcctttg ctcaagaacc cttggatgca gccattcaga agatttctcc
    19501 agaattgttt gaccaatatg aaatctttaa atcacgtgaa atgttgctag aatggtcacc
    19561 aaagaatgtt cataaagcaa caggtttggc aaaactaatc agccatcttg gaatcgacca
    19621 aagtcaagtg atggcttgtg gtgacgaggc caatgacctc tctatgattg aatgggcagg
    19681 tcttggtgtt gctatgcaaa acgctgttcc tgaagtaaag gcagccgcaa atgtagtgac
    19741 gccgatgacc aacgatgagg aagctgtcgc ctgggctatc gaagaatatg tgctaaagga
    19801 gaactaagat atgggattgt ttgaccgtct attcggaaaa aaagaagaac ctaaaatcga
    19861 agaagttgta aaagaagctc tggaaaatct tgatttgtct gaagatgttg atcctacctt
    19921 cacagaagtt gaggaagttt ctcaggaaga agcagaggtt gaaattgttg aacaagctgt
    19981 gttccaagaa gaggaaatcc aagacacagt tgaagaaagt caggattcag agccagctgt
    20041 aggggtttct caagaagaaa tagaagaatt accaaactca gaagaattac cagaggaaga
    20101 gaagcttgag cacgaaggaa ctgtagaaga aaataattct gaagtgcttg aaccagaagc
    20161 tcctcaaaca gaagaaactg ttcaggaaaa atatgaccgc agtcttaaga aaactcgcac
    20221 aggtttcggt gcccgcttga atgccttctt tgctaacttc cgctctgttg acgaagaatt
    20281 tttcgaggaa ctggaagaac tgctgattat gagtgatgtt ggtgtccaag tcgcttctaa
    20341 cttaacggag gaactacgtt acgaagccaa gcttgaaaat gccaagaaac ctgatgtact
    20401 tcgtcgtgtc atcattgaga aattggttga gctttatgaa aaggatggta gctacgatga
    20461 aagcatccac ttccaagata acttgacagt tatgctcttt gttggtgtga atggtgttgg
    20521 gaaaacaact tctatcggaa aactagccca ccgctacaaa caagctggta agaaggtcat
    20581 gctggttgca gcagatacct tccgtgcggg tgcagtagct cagctagctg aatggggccg
    20641 acgagtagat gttccagtag taactggacc tgaaaaagct gatccagcca gcgtggtctt
    20701 tgatggtatg gaacgtgccg tgactgaagg tatcgatatt ctcatgattg atactgctgg
    20761 tcgtctgcaa aataaggata accttatggc tgagttggaa aagattggtc gtattatcaa
    20821 acgtgttgtg ccagaagcac cacatgaaac cttcttggca cttgatgcat caacaggtca
    20881 aaatgcccta gtacaggcca aagaattttc gaaaatcaca cctttaacgg gaattgtttt
    20941 gactaagatt gatggaactg ctcgaggagg tgtggttcta gccattcgtg aagaactcaa
    21001 tattcctgta aaattgattg gttttggtga aaaaatcgat gatattggag agtttaactc
    21061 agaaaacttt atgaaaggtc tcttggaagg tttaatctaa tcagaagcaa aaatcctgca
    21121 aggcataaac ttgcaggaaa tttttttatt ctaagcgacc atcttgacga taggtgatat
    21181 ctggttgcca agtccatttg gcaccgaatt tttcaagtag gtcaaagctg gcttgaggtc
    21241 ccatgcttcc agctttatag tcatgaagtg gggcaccatt ttcagcccag agcttttcaa
    21301 tacggtcaat caacttccat gacgcacaaa cttcatccca gtggctaaag ttagttgagt
    21361 tgttatttag gacatcataa atcaattttt cgtatggttc tggagaagca ccagttgcag
    21421 tcgcatctgt acggtaatca agtgagttag gagccaagtt aaattcttct cctacttgct
    21481 tcccatttag gctaagagag aagccttctg ttggttgaat atagatggtc aaaatatttg
    21541 gagcaagtgg ttctccaaag atagaatcca tttgtttaaa gacgatgttg acatgggttc
    21601 ctttttcagt cagtcgttta cctgtacgga aaaagaaagg aacaccacgg aatcgatcgc
    21661 tgtctacaaa gaaggcacca gatgtaaagg tttcagttgt tgattctgga ttcacatttg
    21721 gctcgctacg ataagagatg tatttcatgc catcaatctt accagagcgg tattgcccac
    21781 ggataaagtg ttctttgagt tcttcatcag ttggatgata gaggttttta aagaccttaa
    21841 tcttttcagc acgaatctcg tcttttgtga agcttgctgg tttgtccatg gcgaggagcg
    21901 aaagaagttg tagagtgtgg ttttggacca tgtcacggag ggcaccggat tggtcatagt
    21961 agccaccacg ttcttctaca cccaagcgct ccgcaaaggt aatttgaaca ttgtcgataa
    22021 aatccttgtt ccaaacgttt tcaaaaatca agtttgcaaa gcgaactgca aagatgcttt
    22081 ggatcatttc cttaccaaga taatggtcga tacggaaaat ttgttcttcg tcaaatgttg
    22141 ctaggagttc gtcattcaac ttgcttgcag ttgcgtaatc tgtaccaaat ggtttttcaa
    22201 cgatcaagcg ctcaaaacct ttgccatcga caatgttttc agatttgagg tgtttggcaa
    22261 tggttccaaa gaactgaggt gccatagaca agaagaagag cttattgtgt tcagcttggt
    22321 atttttcatt aagctcagct tgtaattgac gcaaagcaat ataatgttcc gaatcattga
    22381 catcatggct ttgatagtag aagtggctag caaattcttg ggcttgctcg gtactatctg
    22441 ccaaatcaag gatggactcg acaactacag attcaaaata ttccttactc caaggtctac
    22501 gggcagttcc aataacggca aagtgcttgg aaagattgcc ggattgatat agtctaaaaa
    22561 gggaagggta gagcttgcgt ttagccaggt ctccactcgc accgaaaatt gtaacaataa
    22621 ccttagatga catctagcta cctaatttct atttttattc ctagtcttgc taggtatgag
    22681 gaaacctcat ttcataaact taattctaac ataattttcc gaaaaatcaa aaattccaat
    22741 acgaggtaga aaaactgcaa ggaaatcctt acagtttgag tttagacgtt taagacctta
    22801 tctaagaact ctttcagacg agggtgttgt gggttatcaa agatttggtc aggtgttccg
    22861 tcttcaagga actcgccatc tgcagtaaag ataacgcggt tggcaacctg acgagcaaat
    22921 cccatctcat gggttacgat aatcatggtc atgccttgct cagccaattc cttcataacg
    22981 ttaagtacgt ctccaaccat ctcagggtca agggcagaag ttggttcatc gaagagcatg
    23041 atgtctggat tcattgctag gccacgagcg atggccacac gttgtttttg accacctgat
    23101 aggctatctg gattggcatt agctttatct gctagtccaa ccttttcaag caactccatt
    23161 cccaattcct cagcttcttc cttagtcatc aacttgtgct caataggagc aaaggtgatg
    23221 ttgtccaata cagacatatg agggaagagg ttgaagtgtt ggaataccat gccgatattt
    23281 tcacggacgt ggtcaacatt ggttgttttt tcagttaaat catagccgtt cacagtgatg
    23341 tgaccgctag tgacttcttc taaaagattg aggctacgga ggaaagttga cttaccagaa
    23401 cctgaaggac cgatgataca aacaacatct ccttcataga acttagtcgt aattcctttt
    23461 aggacttcat tttttccata gtgcttgtgt aaatcattta catcaatttt taattttacc
    23521 attaacgaat cctcttttct aagcgtttcg ctagtctagt caaaagtgtg ataattacaa
    23581 gatagaagat agcaaggatt gcatacatct tgaaactttg gtagttacga gcaatgataa
    23641 tcttaccagt ttggaagagt tcaaccaaac cgatagcaga tacgatagtt gtatctttaa
    23701 gagcgataac gaattggttg acaaagtttg gcaacatcaa tttagttgct tgtggcaaga
    23761 taatcttacg catggttttt ccataagaga tacccaagct tcggctggct tccatttggc
    23821 caactggaac ggcctgaata ccaccacgaa cgatttcagc gatataagca gccgcattga
    23881 gtgagagggc aatggttcca gctacaaagt cgttaattgg gctttgttgg cctgtgatag
    23941 actcgatgaa gtttggaatt ccccagaaga tgaaggctgc aagaatcatc aatggaatac
    24001 cacgaataac gtcaacgaaa atctcagaga tgacgcgaag agatttgtat gggctaacgc
    24061 taaacatacc gaagataatt ccgatgacaa tggcaatagc aaatgagata agagctagag
    24121 caagagtgat accaagaccg ctaaggagtt gtttgtagtt gttttgaagc aagccccaga
    24181 gcgttgtttc gtcaacagta cttgttgaag cagttgaaga ttcgctagct aggtatttgt
    24241 caagaatctt ttggaattca ccgtttgctt taaggtttgc aagtccgttg ttgaacattt
    24301 caatcagttc tggatttgct ccttttttaa cggcaaaggc tgtttcaccg attggagttc
    24361 cagagattgg agttttcaat ttttgacctt ggctgataga atatttgaga acaggttcat
    24421 catccataac ggcatcaatg gcaccagtgt ttaaactgtc atacattgaa gaaccatcag
    24481 caaaggtttt gattttgtag ccgtatttgc tttgattttc tgttaggaag gtttgagaag
    24541 cagttccgtt tttaacaccg actgtctttc cttttagatc ttcataagaa gcaatattgc
    24601 ttgattcttt gacaccaaga atggtattag cagtgtagta tgattctgag aagtcaaaag
    24661 ttgccttacg agcatctgtg acagacatac cagcgatgat accatcggct tgaccagctt
    24721 ggacagcact gatagcagca tcaaaaccag ggttggtgat ttcaatttca aaaccttggt
    24781 ctttagcgat tgccttaatc aattccatat caataccagt gtattggttg cttgaatttt
    24841 ggaaaacaaa aggtgcaaaa gaagaatcgc tggcaatgat atatttagcc ttaacaggaa
    24901 tagcttttaa tcctgcgaga gtagttgtag ttggcactgc tgaagatgat gaagcagtcc
    24961 atttcttgat aattttatca agactaccat cttttttcat ttcagacaag gcttggttaa
    25021 attcagtaac caggtgctcg tatttacttc cttttttcac accgaaagca aaacttccta
    25081 cagcttcacc atccatttca atatggaggt cttgaccttg gttaatggca tattcgataa
    25141 caggtttgtc atccatcatg gcatcgatgg caccagcact caagctgttg ttcattaaat
    25201 caccagtgtc aaatgtttta atagtaaagc cgtatttatc tttgattgtt tcaaggaaac
    25261 gttgagcggc agttccgttt ttaacaccaa cggttttgcc agttaattgg tcgtacttgc
    25321 taattttgtg tgactttgta gtagcaatga caacttttgt atcatagtaa gtatcagaca
    25381 tggtgaagac tttttcacgt tctttagtct ttgtcatccc tgccatgata gcgtcggctt
    25441 gcccagcttg aaccgcattg actgctgcgt caaatccagg ataggacatc tgaatgttcc
    25501 agcctttaat ctcagcgact ttgttaataa tgtcaacatc aattccttta taagtttgat
    25561 ctgaatcttt aaactcaaaa ggtgcatagg cggtatcaga aacaatctta atcgtttctg
    25621 ctttggcaat acctaatgag aaaattggga ataaaattag caaaaatgct agaaattttt
    25681 tcttcatttt tagtctcctt ttccgaatat tctctcatta tatcagaaaa agtaaaaaaa
    25741 ggcaaatttt tatatttttc gtgtcagaaa atataacatc gattttttcc tttccttgaa
    25801 ttgctattaa aaagtgctat aataatagta tatatagaag aaaagaggac ggggatatga
    25861 agaacaaaag aatatttaaa gacttccaag cttcaaaaat gagtttaaac atttacacaa
    25921 gccccttgtt agcctttgtt tttgtcttca taggagagtt tgtggctttt actttgtatg
    25981 gtattggctt gttagctctc atcggacttg ctagaaattt tggagaggct ggtcaaaatc
    26041 ttgcaagcta cttgcagacc ttgcatcaga gcttgacgga taaaacaagt gactttcgtt
    26101 taattttagg attactggcc tttggtttta ttcttaacac tgtgttcaga tggacaagaa
    26161 aagttgagaa aagacctatt cgaaccttgg gattttatag agagaatttc ctcagcaatc
    26221 ttctgaaagg atttagtcta ggcctggcac tttttcttct gaccttgtta ggtttagtgg
    26281 tcttaggtca atatcgtttg gaatccattc acttgaatcc ttattctctt gcctttgtcg
    26341 tctttactat cccattttgg attttacagg ggacagcaga agaagtggtg gcccgtgctt
    26401 ggctacttcc tcaattggcc tcaagaacca atctaaaact agctattctt atatctagcc
    26461 tgttctttac cctgcttcat atgggcaatt ctggtctcac ccctctatct ctagtaaatc
    26521 tctttttatt cggagttgcc atggctcttt accttctcaa aactgataca gtttggggtg
    26581 ttgcaggtat tcatggtgct tggaattttg ctcagggtaa tctctttggg attttagtta
    26641 gtggtcaacc gtcaggaacg tctctgatga cctttttacc acaaggcaat caagattggc
    26701 tatcaggtgg ttcttttggc atagaaggtt ccattatgac aagtctggta ttactactgc
    26761 tgattgtcta tcttgctaat aaattaaaga aagaaaatga aaggatgtga cttcggtccg
    26821 tccttttctt cgtgaaaata ctataagtat gctaaaatag gaatagcaca tggagagagg
    26881 attcttatga tcaatcacat tacagataat caatttaaac tagtatcaaa atatcaacca
    26941 tcaggagatc aaccccaagc tatcgagcag ttggtggata acattgaggg gggagaaaaa
    27001 gctcagattc tgatgggggc gactggaaca gggaagacct atactatgag tcaggtcatt
    27061 tctaaagtca ataaaccaac tctggttatt gcccacaata aaactctggc tggtcagctc
    27121 tatggggagt ttaaggaatt tttccctgaa aatgcagttg agtatttcgt atcctactat
    27181 gattattacc agccagaggc ctatgtccct tctagcgata cctatattga gaaggatagt
    27241 tctgtcaatg acgagattga caagcttcgc cactcagcta cctcagccct tttggagcgt
    27301 aatgatgtta ttgtcgtggc ctcagtctct tgtatctatg gtttgggttc gcccaaggaa
    27361 tacgctgata gtgtcgttag tctccgtcct ggtctagaga tttctcgtga taaactcttg
    27421 aatgacttgg tcgatattca gtttgaacgt aatgatattg atttccaacg cggaagattt
    27481 cgcgttcgtg gggatgtggt agagattttc ccagcttccc gagatgaaca tgcctttcga
    27541 gtagaatttt ttggagacga aattgaccgt attcgtgaag ttgaggctct gacaggtcag
    27601 gtgttgggag aagtggatca tttagcgatt ttcccagcga cacactttgt gaccaatgac
    27661 gaccacatgg aagttgccat tgcaaagatt caggccgagt tggaagaaca attagctgtc
    27721 tttgaaaagg aaggtaaact gcttgaagcc cagcgtttga aacagcggac agagtatgat
    27781 atcgaaatgt tgcgtgagat gggctatacc aatggggttg aaaattattc tcgccacatg
    27841 gatggacgga gcgaaggaga gcctccttat acgcttctcg acttcttccc agatgatttc
    27901 ttgattatga ttgacgagag tcatatgacc atagggcaaa tcaagggcat gtacaatgga
    27961 gaccgttcgc gtaaagaaat gctggttaat tatggtttcc gtttgccgtc tgctttggac
    28021 aatcgtcctc tccgtcggga ggagtttgag agtcacgttc atcagattgt ttacgtttca
    28081 gcgacacctg gtgactatga aaatgaacag accgagacag tgattgagca aatcattcgt
    28141 ccaacgggac tcttggatcc agaggtggaa gtccgtccga ctatgggaca gattgatgac
    28201 ctcttgggtg aaatcaatgc ccgcgttgaa aaaaatgagc gtacctttat cacaactttg
    28261 accaagaaaa tggcagagga tttgaccgac tacttcaagg aaatgggtat caaggtcaag
    28321 tacatgcact cggatatcaa gaccttggaa cggacggaga ttatccgtga cctgcgcttg
    28381 ggtgtctttg atgtcttggt cggaattaac ctgctccgtg aaggaattga cgttcctgaa
    28441 gtgagcctcg tagctattct cgatgctgac aaggaaggtt tccttcgcaa cgaacgtgga
    28501 ctcatccaga ctattggacg tgctgctcgt aacagtgaag gccatgtcat catgtatgca
    28561 gacacggtta cccagtctat gcaacgtgct atcgatgaaa ctgcccgccg tcggaaaatt
    28621 cagatggcct ataatgaaga gcatggtatc gtaccacaga caatcaagaa agaaatccgt
    28681 gacctgattg ccgtgaccaa ggcagttgct aaggaagagg acaaggaagt cgacatcaat
    28741 agcctcaaca aacaagagcg caaagaactc gtcaagaaac tagaaaaaca aatgcaagaa
    28801 gccgtcgaag tgcttgactt tgaactggcg gctcagatcc gtgatatgat gttggaagtc
    28861 aaggccttgg attaggggaa tagtatgatt tatttaagaa agttaaagag agaagatttg
    28921 atgtctttat gggaaatggc ttattcacaa cttaatccag tttggaaaca gtatgatgct
    28981 ccctattatg atgattatca gtatttttca aattttaaag aattcgaact acaaaaatca
    29041 gaatccattt taagcaactc aaatcgcctt ggtatttttg ttgatgataa actagttggg
    29101 actgtttcgc gttattgggt atgtaaacaa acaagatgga tggaattggg aattggtatt
    29161 tatgataaaa aattctggaa cactggtatt gggaaagttg ctatgttgca gtggatagat
    29221 aggacgtttc aggattactt ggagttggag catctgggtt tgacaacttg gtcagggaat
    29281 attggtatga tgaaacttgc tgaaaaatta agaatgaaaa aagaagctca tattccaaaa
    29341 gttcgttatt atcaaggtaa atattttgat agtattaaat atggtatttt gagagaagac
    29401 tgggagaaaa taaatgacgg ttattatcaa atcaatggaa actcctaaag agatagaagg
    29461 taaatccctt gttcactggc aaacgtggag agagtcttat gatgaccttt tgcctgcgga
    29521 atttcaggat acaatgacat tagaaagatg tcgattcttt agtcaaaagt atccagaaaa
    29581 tacattgatt gcgatggatg gcgtgaaggt ggttggtttt atcagttatg gcaattttcg
    29641 tgatgagact attcaagctg gtgaaattat tgctttatat gttttaaaag actattatgg
    29701 aaaaggtatc gcacaaaagt taatgaaagc agctttgact gctcttaatc atttttctga
    29761 aattttctta tgggtattga aagataacaa gcgcgccatt gctttctatc aaaaaatggg
    29821 ttttactttt gatggacaag aaaaaatact tgaacttgga aagcctataa aggaaaaacg
    29881 aatggtgttc tattctaaat aattttaaaa aagtaaaaga taatatctct ggtagtaagt
    29941 ctgaaaattt aataaattag aaagcgagta aatttatgtc ccgttcccaa ttaacaattt
    30001 taacaaatat ctgtctgatt gaagacctcg aaactcagcg cgtggtgatg cagtatcgcg
    30061 cccctgaaaa caatcgctgg tctggttatg cctttcctgg aggtcatgta gaaaatgatg
    30121 aggcttttgc ggagtctgtc attcgtgaaa tctacgaaga aacagggttg actatccaaa
    30181 atcctcaact tgtcggcatt aaaaattggc cactagatac aggtgggcgc tatattgtca
    30241 tttgttataa ggcgactgag ttctctggta cccttcaatc ttcagaagag ggagaagttt
    30301 cttgggtgca aaaagaccag attccaaact taaatctggc ctatgatatg ctaccatttg
    30361 atggaaatga tggaagctcc cgacaagtca gagtttttct atcctcgccg tacagaagac
    30421 gattgggaaa agaaaatctt ctagtctttt actaaataac ctagctgatc caaggcctcc
    30481 tcgatatagt ggaggtcttg ttgtgtttcg gcttcgacta ggtggtaatg gatgccatct
    30541 gtcaattcag atagaggtct aaagtcagaa tgctcgactt gttctagaaa atgttgcaca
    30601 tctctgcgac aagacagttt aagcaaggtt tcaatttctc cataaacggg atggtcaatc
    30661 aaggtattct ggacatgccc accattatcg acaatagcaa ggagttcctg accgatttct
    30721 tcaacttcat gtttcacttt gaaaagtttg tgaacataag gattggtatc aatttgctta
    30781 tagatgtagc cacgattggt ggataggata ggagcgccat cggctctcaa aattgcaatg
    30841 tcctgcacaa tgacctgacg tgtgacatga aaatgttccg ccaaactttg gccattaagg
    30901 gctttaggag cttctttcaa gagttggagc agggcttgtt tgcgatcttt tgtcatagct
    30961 tttcctttta acggcgtttt cgaagcactt tatagacagc tagtgctaat gtatagtcta
    31021 ccatactatg gataattgtg ccaaatccaa ctagtacaaa tagaacatag aacatatttt
    31081 ctacattggt acccgaagtt gcgtaaaaaa ggacacaggc taatacttca gcaagggcat
    31141 gaacaacagc caaaacaaaa ttgaaaaccc aggaagattt tggtttatct agggtatcgg
    31201 ggaatttttg taggtaaaga gctcctaaag caccaaaaga tatatgggaa aaagcccgaa
    31261 aaacgataac catgggatag ccagccatca aaaatccaaa actagaggct aggatgacaa
    31321 aaactgccat caagggcgac aagaacatgg ctataaaaat agcgatgtgg ctccccaaag
    31381 tataggaagc aggtggaatg acaatcttga aaggcataac aattggaatc aaaatcgcaa
    31441 tagccgttaa aagggctgtc attgtcataa attgtgtctt tttccgtgta ttcacaagaa
    31501 tctccttttt aactgcatat acactagtat ggtacaataa accagacaat aaagcaagaa
    31561 tttacttggg tttatagatc attttttagt taaaagttat agtagattga aactagaata
    31621 gtccacctct acttctaaaa cattgttaga aatcgatttg actgtcctga tcgatttgtc
    31681 ctgttcttat ttcgttttac tatagtaaag atttcattaa aaagaaactg tatagagcaa
    31741 aatctccacc ttcaggtttg aaagcggaga ttgtttttat tttttcaagg tttgtagtcg
    31801 tggaacaatg gctagggtta gaattgcaga aatgaccaat tcagcaattg aatttgttga
    31861 gataacggtt gccagaagaa gttggatatt tccattataa acatttccaa ataggaagaa
    31921 gattcctcca aggacaaaga tagtatttgt caaggaacca agagccccag ctaaaatcag
    31981 accagtcttg tttttcatca gtttatagac taagtaagga gttaaaccaa tcaaaatacg
    32041 tgggacaatg gcaatgatag ctgagtagat gtttccgttt ggtacgaagg gagagaagag
    32101 gtagcttgtc ggtagaatcg taatcgtgtt aaccgtcaag ctaagtaatc ccatcaaaaa
    32161 tccaagtgta accccaactc gtggaccata aataatgctg gcaataatga caggaatatg
    32221 aacaatggtc ggtttgattg gaaatggaaa aaggttaaag ataagtgagc tcagaaagtg
    32281 tatcacgagc atggtagcaa aaaagatagc aatgggtgca atattagagc gttttttcat
    32341 cgatagtttc ctttattctt tctaaaataa ttgtgaggtc agctaaagct cctcgtccgt
    32401 ggtctccaca agctagtagg gattccttag gagcaatcag ctgatagccg taggtttcta
    32461 atgttttcag attattctga gttactggat ggtcatacat ttttgtattc atagcaggag
    32521 ctattagttt gggaatatga cttggtaggg ctagagctgt actggttacc atgttgtccg
    32581 caaatccgtg agctagtttt gcaatagtgt tagcagttgc aggtaccacg ataaataaat
    32641 ctgctttttt tccaagttcg atatgattga cctgatcagg atagggttct ttcatgacat
    32701 ccaagtggac aggattctgt gagagtacct gtagtgtcaa aggttggata aactctgtag
    32761 cagcctgagt cattaagaca gtgacttgat ggccttgttt ttttagagaa ctgactaaat
    32821 ctgccgactt ataagaggcg attgaacccg ttacagccaa gagaatgttt gccatagttt
    32881 tcctttctaa gaatgatagg cttgaatttt ttcaaggagg agttctgcaa tttcttcttt
    32941 agtctggact gtttgaagct gatttttctc aacaaatata gctcggtgct gacctgctga
    33001 aatttgagtc aggtcattcg cgatgattaa atctgcttga ttcttgataa gactttttcg
    33061 tgcaatgtca accagatgat cttcggtaac atcaaccagc agtttgaaac caatcagatg
    33121 aatagtagga ttccattcct tgactaggga tatgattttg ggtgtctttt taaggaacaa
    33181 aacctgaacc tcatcagttg aagaaatctt ggcctgatga ttctgcttgc ttaaaaattc
    33241 ttttagattg gagctagcct gaacttcctc aagccctgtc atataaacag gagtgtagtc
    33301 agaaacagcc attgagtgga tcaagacctg ataatcctga acacgttctt gcatttctat
    33361 tagaaggtcc ttggtattgg taatttctcg aatacttagg ttaggatgag gctctggctt
    33421 cagagctcgt tttgtcgtaa ttaaacaaac ttcatgccct gcagaaagca aagtctctgt
    33481 gataattttc cccaagtgac ctgtagaatg gttagtgata gagcggacgc tatcgatagc
    33541 ttcactggta ccgcccgatg taactaaaat tttcatagca ctattgtaca caaaaataag
    33601 cggtaagtaa aatgaaagtg ataaattttt ggtaaaaacg aagatttact atagtttcaa
    33661 actataaagc catataaaat ttaagaaagg actctaaaag tcttaaaaac agagatattt
    33721 acgaacgttt agagagtttt aagatgttaa aaatcttgtt ttgtgatata atgaattatc
    33781 atataagagg ttagaggaat tttgaatgaa aacagatatt gaaatcgcac agagtattga
    33841 gttgaagcca attgttgatg ttgtagagaa acttggtatt tcttacgacg atttggagtt
    33901 gtacggaaag tacaaggcta aactcagctt tgataaaatt cgggcagttg agagcaatcc
    33961 agtcggtaaa ttgatcttgg ttactgccat caacccaaca cctgcaggtg aaggaaagtc
    34021 gacgcttacc attggtcttg cggatgcctt gaacaagatt ggcaagaaaa ccatgattgc
    34081 tatccgcgaa ccgtctcttg gtccagtcat ggggatcaag ggtggtgctg ctggtggtgg
    34141 gtatgcacaa gttctgccaa tggaagacat caacctccac tttactggag atatgcatgc
    34201 tattacaact gccaacaatg ccctttctgc cttgattgac aaccacttgc accaagggaa
    34261 tgagctggga attgatcaac gtcgtatcct ctggaaacgc gttgtggact tgaacgaccg
    34321 tgcgctccgc catgtgactg ttggtcttgg tggtcctcta aacggtattc cacgtgagga
    34381 tggttttgat attacagttg cttcagaaat catggcaatt ctttgcttgg caacggacat
    34441 cgaggacttg aaacgtcgtt tggcgaatat cgttattggt tatcgctatg accgtacgcc
    34501 tgtttctgta ggtgatttgc aggttgaggg tgccttggct ttgattttga aggatgctat
    34561 taagccaaac ttggttcaga caatttacgg tacacctgcc tttgtacacg gtggtccatt
    34621 tgccaatatc gctcatggct gtaactctgt tttggcgacg acaacagccc ttcacttggc
    34681 tgattacact gttactgaag ctggttttgg tgcggacctt ggtgctgaga aattccttga
    34741 tatcaagaca ccaaacttgc caacatctcc agatgcagtt gttattgtcg caacccttcg
    34801 tgcccttaag atgaatggtg gtgtggctaa agacgctctg actgaagaaa atgtagaggc
    34861 agttcgtgca ggttttgcta acttgaaacg ccacgttgaa aatatccgta agttcggtat
    34921 tccagcagtt gtagctatta acgaatttgt atctgataca gaagctgaaa ttgcagtctt
    34981 aaaagaactc tgtgcctcaa tcgatgtacc agttgaattg gctagtgtct gggctgatgg
    35041 agcagaaggt ggagtagcac ttgccgaaac agttgttaag acaattgctg aaaatccagc
    35101 taactataaa cgtttgtacg ataatgacct ttctgtccaa gaaaagattg aaaaaattgt
    35161 cactgaaatc tatcgtggta gcaaagtgaa ctttgagaag aaatctcaaa cacaaattgc
    35221 tcaaatcgtt caaaacggtt gggacaaatt gccaatctgt atggctaaaa ctcaatacag
    35281 tttctcagac aatccaaatg cgcttggagc accagaaaac tttgaaatta ccattcgtga
    35341 attggtacca aaattaggtg caggcttcat cgttgcctta actggtgatg ttatgaccat
    35401 gccaggtctt ccaaaacgtc cagcagctct caacatggat gttgaaagcg atggaactgt
    35461 actaggcttg ttctagtgga ttgaaattga ctttaagtga gtttggaaat aatatccaag
    35521 ctcatttttt ctcagactgc aagcagttca tcaataagat ttatactctt cgaaaatctc
    35581 ttcaaactac gtcagcttca ccttgccgta ggtatatgat actgactctg tcagttctat
    35641 ccacaacctc aaaacagtgt tttgagcaac ctgcggctag cttcctagtt tgctctttga
    35701 ttttcattga gtataagtag atagaaaagt tatatactat ctattgaaaa tattataaat
    35761 cagatacaaa gacaggtgaa accttctgtg ttatactaga gatatgttag atttgaaaga
    35821 atacggtatc gtcatgtggc cggaggagaa agtcatttct ttccgtgaga aacttctcgc
    35881 ttggtatgat gaaaacaaaa gagatttgcc ttggaggaga agtaaaaatc cttatcacat
    35941 ctgggtatct gaaatcatgc ttcagcagac cagggtggat acagttatcc cttactacga
    36001 aagattcctg gactggtttc caactgtcga aagtctggca actgcgcctg aggagagttt
    36061 actgaaagct tgggagggct tgggctatta ttctcgagtt cgcaatatgc aggctgcagc
    36121 ccagcagatt atgactgact ttggtggcca atttccaaat acctatgaag gaatttccag
    36181 cttgaaaggg attggacctt acacagcagg agccatttcc agtattgctt ttaacttgcc
    36241 tgagccagct gtagatggta atgtcatgcg ggtcttggcg cgtctgtttg aagtcaacca
    36301 cgatattggg attccaagta atcgaaaaat ttttcaggca atgatggaga tcttgattaa
    36361 tccggatcgt ccgggtgatt ttaatcaagc cttgatggac ttagggtctg atattgagtc
    36421 tcctgtaaat cccagacctg aagaaagccc agtcaaggac tttagtgcgg catatcagaa
    36481 tggaaccatg gaccgttatc caattaaatc tcctaagaaa aagccggttc ctatttatct
    36541 taaagcctta gttgtgaaaa attctcaagg acaattttta cttgaaaaaa atgaaagcga
    36601 aaagctcttg gcaggttttt ggcatttccc ctttatagaa gttgataact tttcgcaaga
    36661 agagcagttt gacctctttc atcaggttgc ggaagaaagt gtgaactttg gccccagtcc
    36721 agaagagagt ttccagcagg actatgacct agatgttgat tggcttgatg tttgttttga
    36781 tacagtccag catgttttta gtcatcgaaa atggcatgta cagattgtag caggtcaggt
    36841 gagtgacttc catgattttt cagataggga agttcgttgg ctttcaccag aagaattcaa
    36901 gaattaccca cttgccaaac cccaacaaaa aatctggcag gcttatgcac aagccaactt
    36961 agacagtagc caagactagc tattgtgtct tctgactatt ttttggtata atagtagaga
    37021 aaaaggtgaa catatgaaaa aaatactaat tgtagatgat gagaaaccaa tctcggatat
    37081 tatcaagttt aatatgacca aggaaggtta tgaagttgta actgctttta atggtcgtga
    37141 agcgctagag caatttgaag cagagcaacc agatattatt attctggatt tgatgcttcc
    37201 agaaattgat ggtttagaag ttgctaagac cattcgtaag acaagcagtg tgcccattct
    37261 tatgctttca gccaaagata gtgaatttga taaggttatc ggtttggaac ttggggcaga
    37321 tgactatgta acgaaaccct tctccaatcg tgagttgcag gcgcgtgtta aagctcttct
    37381 gcgtcgttct caacctatgc cagtagatgg tcaggaagca gatagtaaac ctcaacctat
    37441 ccaaattggg gatttagaaa ttgttccaga cgcctacgtg gctaaaaaat atggcgaaga
    37501 actagactta acccatcgtg aatttgagct tttgtatcat ttagcatcgc atacaggtca
    37561 agtcatcacg cgcgaacact tgcttgagac tgtctggggt tatgactatt ttggtgatgt
    37621 ccgcacagtt gatgtgactg tacgacgtct gcgtgagaag attgaagata cgcccagccg
    37681 accagagtat atcttgacgc gccgtggtgt agggtattac atgagaaata atgcttgatt
    37741 tactgaaaca aaccattttt accagagatt ttatctttat cctgattttg ttaggtttca
    37801 tccttgttgt gaccctctta ttactggaaa atagacgtga taatattcag ttgaagcaaa
    37861 tcaatcaaaa ggttaaagat ttgattgcag gagattattc caaggttctt gatatgcaag
    37921 gtgggtctga aatcaccaat attaccaata atttgaatga cttgtcggag gttattcgtc
    37981 tcactcagga aaatctagaa caagagagta agaggctaaa tagtattctg ttttatatga
    38041 cagatggggt tcttgcgact aaccgtcggg gtcagattat catgattaac gatacagcca
    38101 agaagcaact ggggttggtt aaggaagatg ttctgaatag aagcattttg gaattgctca
    38161 agatagaaga aaactatgaa ttgcgtgatt tgattaccca aagtccagaa ttgttgctag
    38221 attcccaaga tatcaatggc gaatatttga accttcgagt tcgctttgcc ttgatacgtc
    38281 gagagtctgg ctttatttca ggtttggtgg ctgttttgca tgatacgacg gagcaggaga
    38341 aggaagaacg cgaacgaaga ctctttgttt ccaatgttag ccatgagtta cggactcctc
    38401 tgactagcgt aaaatcctat cttgaagcct tggatgaggg ggctttgtgt gaaactgtag
    38461 caccagactt tatcaaggtt tctcttgatg agaccaaccg tatgatgcgc atggtgacgg
    38521 atctcctcca tctttcacgt attgataatg ctaccagtca cctagatgtg gaactgatta
    38581 acttcactgc ttttattacc tttatcctca atcgttttga caagatgaaa ggacaggaaa
    38641 aggagaaaaa atatgagctg gtgagagatt atcccatcaa ttctatctgg atggaaattg
    38701 atacagataa gatgacgcag gttgtcgaca atattttaaa taatgctatt aagtattcgc
    38761 cagatggggg taaaatcact gtcagaatga agacaactga agaccagatg attttatcca
    38821 tttctgacca cggtttgggg attcctaagc aggatttacc acgtatcttt gaccgtttct
    38881 atcgtgtgga tcgtgctaga agtcgtgcac aaggtggtac aggtctagga ctgtctatcg
    38941 ctaaagaaat tatcaaacaa cataagggct ttatttgggc caagagtgaa tacggcaagg
    39001 gttcaacctt taccattgta ctcccttatg ataaggatgc agtgaaagaa gaagtatggg
    39061 aggatgaagt agaagactag aatgagtgaa ataggcttta aatacagtat tttagcgtcg
    39121 ggttccagtg gaaattcttt ttatctggaa acctcaaaaa agaagctttt agtagatgca
    39181 ggcttgtctg gcaagaaaat taccagtctg ctagctgaaa ttaaccgtaa gccagaagac
    39241 ctggatgcca tcttgattac ccatgagcat tcagatcata tccatggagt aggcgttttg
    39301 gctcgcaagt atggtatgga tctttatgcc aatgaaaaga cctggcaagc tatggaaaat
    39361 agtaaatatc ttggcaaggt ggattcttcg caaaagcata tttttgaaat gggtaaaacc
    39421 aaaacctttg gagatatcga catcgagagt tttggtgtaa gccatgatgc agtcgcaccg
    39481 cagttctatc gctttatgaa ggatgataag agttttgtcc tcttgacaga tacaggttat
    39541 gtcagtgacc gtatggcggg cattgtcgaa aatgcggatg gttatcttat cgaggccaac
    39601 catgatgtag agattttgcg atcaggttct tacgcttggc gactcaaaca acgaatccta
    39661 tctgaccttg gtcacctttc taacgaggac ggtgctgaag ctatgattcg gacgctagga
    39721 aatcgtacta agaagattta ccttgggcat ttatctaagg aaaacaatat caaggaactg
    39781 gctcatatga ccatggtcaa tcagctggct caagctgatc tgggagtcgg agtagacttt
    39841 aaggtttatg atacctcacc agataccgca acaccattga cagagatata gaaagaacgc
    39901 tgagaaggtg ttcttttt
//
LOCUS       DAVKMU010000017        34598 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKMU010000017 DAVKMU010000000
VERSION     DAVKMU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.017"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            770..1222
                     /locus_tag="VW343_000981"
     CDS             770..1222
                     /locus_tag="VW343_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9755548.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1241..2578
                     /locus_tag="VW343_000982"
     CDS             1241..2578
                     /locus_tag="VW343_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9755549.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2657..3541
                     /locus_tag="VW343_000983"
     CDS             2657..3541
                     /locus_tag="VW343_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9755550.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3541..4374
                     /locus_tag="VW343_000984"
     CDS             3541..4374
                     /locus_tag="VW343_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9755551.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4392..6485
                     /gene="nanB"
                     /locus_tag="VW343_000985"
     CDS             4392..6485
                     /gene="nanB"
                     /locus_tag="VW343_000985"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9755552.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWRYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6497..7600
                     /locus_tag="VW343_000986"
     CDS             6497..7600
                     /locus_tag="VW343_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9755553.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQLSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7773..8471
                     /locus_tag="VW343_000987"
     CDS             7773..8471
                     /locus_tag="VW343_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9755554.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8490..10007
                     /locus_tag="VW343_000988"
     CDS             8490..10007
                     /locus_tag="VW343_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9755555.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            10132..11460
                     /locus_tag="VW343_000989"
     CDS             10132..11460
                     /locus_tag="VW343_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9755556.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11712..12578
                     /locus_tag="VW343_000990"
     CDS             11712..12578
                     /locus_tag="VW343_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9755557.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12594..13433
                     /locus_tag="VW343_000991"
     CDS             12594..13433
                     /locus_tag="VW343_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9755558.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13705..14157
                     /locus_tag="VW343_000992"
     CDS             13705..14157
                     /locus_tag="VW343_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9755559.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14290..14598
                     /locus_tag="VW343_000993"
     CDS             14290..14598
                     /locus_tag="VW343_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755560.1"
                     /translation="MDGQGITVDEMAWIIRGIVNGLIGRYIKLGTYAAKYGISMARSI
                     LSRAAATAAARVGLSTKISGWILRVAVNVADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14608..14907
                     /locus_tag="VW343_000994"
     CDS             14608..14907
                     /locus_tag="VW343_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755561.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDEHTWSTILIASLFGV
                     YDYKPIDKNRKKSKRKKKKEKIDLFMVGTYESFTDKKENSLQRKMMEEQTWHKKE"
     gene            14889..15533
                     /locus_tag="VW343_000995"
     CDS             14889..15533
                     /locus_tag="VW343_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9755562.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15702..16619
                     /locus_tag="VW343_000996"
     CDS             15702..16619
                     /locus_tag="VW343_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9755563.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16637..17521
                     /locus_tag="VW343_000997"
     CDS             16637..17521
                     /locus_tag="VW343_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9755564.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17537..18388)
                     /locus_tag="VW343_000998"
     CDS             complement(17537..18388)
                     /locus_tag="VW343_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9755565.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18588..20645
                     /gene="pbp2b"
                     /locus_tag="VW343_000999"
     CDS             18588..20645
                     /gene="pbp2b"
                     /locus_tag="VW343_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9755566.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20656..21252
                     /gene="recR"
                     /locus_tag="VW343_001000"
     CDS             20656..21252
                     /gene="recR"
                     /locus_tag="VW343_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9755567.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21428..22471
                     /locus_tag="VW343_001001"
     CDS             21428..22471
                     /locus_tag="VW343_001001"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9755568.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22555..23928
                     /locus_tag="VW343_001002"
     CDS             22555..23928
                     /locus_tag="VW343_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9755569.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELGITDLVERKDSLTFKVNFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     RLENEAK"
     gene            23915..24526
                     /locus_tag="VW343_001003"
     CDS             23915..24526
                     /locus_tag="VW343_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9755570.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKFLDGQFQEN
                     QEIADLQFFAIDQLPALSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24617..25321
                     /locus_tag="VW343_001004"
     CDS             24617..25321
                     /locus_tag="VW343_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9755571.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25540..26913
                     /gene="ftsA"
                     /locus_tag="VW343_001005"
     CDS             25540..26913
                     /gene="ftsA"
                     /locus_tag="VW343_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9755572.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26930..28189
                     /gene="ftsZ"
                     /locus_tag="VW343_001006"
     CDS             26930..28189
                     /gene="ftsZ"
                     /locus_tag="VW343_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9755573.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28194..28865
                     /locus_tag="VW343_001007"
     CDS             28194..28865
                     /locus_tag="VW343_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9755574.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28875..29414
                     /locus_tag="VW343_001008"
     CDS             28875..29414
                     /locus_tag="VW343_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9755575.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29414..29677
                     /locus_tag="VW343_001009"
     CDS             29414..29677
                     /locus_tag="VW343_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9755576.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29674..30459
                     /locus_tag="VW343_001010"
     CDS             29674..30459
                     /locus_tag="VW343_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9755577.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30468..31256
                     /gene="divIVA"
                     /locus_tag="VW343_001011"
     CDS             30468..31256
                     /gene="divIVA"
                     /locus_tag="VW343_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9755578.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31604..34396
                     /locus_tag="VW343_001012"
     CDS             31604..34396
                     /locus_tag="VW343_001012"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9755579.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLTSLSEK
                     FGWVDVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEYVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPTQDAVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10664 a         6082 c         7501 g        10351 t
ORIGIN      
        1 attatacgac tattaacctt atcttctcct aaaattgact ttcttgtttt cttatcttgt
       61 ctactcgaaa caagtattgt aagaatttga ttatttttga aagtactttt aatatacttg
      121 atatagttaa aaaagatttg aaactaaatt ccaaattaga aaaagacttg aaatactaaa
      181 aaaaaaaagt atactctaat tgaaaacggt aacaaaacta atttagagaa tgaaatatag
      241 agtatttctc tcttaaaagt ttttggtgaa acgagatgta gaaaggagat ttagccaaag
      301 agtctattag tgctagaata atagattaga attattttag aaaaacgaag tgagcagctt
      361 ataaattcaa gtccccaaat agattcatac tagtatcttt tgcaaaaaat aaagggcgac
      421 ttccttcatg aatatcaatt tcatctataa ggaaggtagc taattgaact aacttattta
      481 ttctgtttgt cgctagaaaa atcagacctc cttgtgaaga ttgaggagat actgaatgaa
      541 aatcaaagaa gaaactagca agctagtagc agattgccca aaacaccgct ttgaggttgt
      601 agataagact gacgtatata atccaaggtg aagcgactgt ggtttgaaga gattttcaaa
      661 gagtataggc tagagagtag tgtttttatg tccttctagt agaaaatgct agacagaaga
      721 atggggaact tggataggaa aaatagattg agaaaggagg ttagaagaga tgattattac
      781 aaaaattagc cgtttaggaa cttatgtggg agtaaatcca cattttgcaa cattaataga
      841 ttttttagaa aaaacaggac tagaaaattt aacagaaggt tcgattgcta tcgatggtaa
      901 tcgattgttt gggaattgct ttacttatct agcagatggt caagcagggg ctttctttga
      961 aacacaccaa aaatatttgg atattcattt agttttggaa aacgaagaag ccatggctgt
     1021 tacatcgccg gaaaatgtaa gcgttaccca agaatatgat gaagagaaag atattgaatt
     1081 atacacaggg aaagtggaac agttggttca tttgagagct ggtgaatgcc tcatcacttt
     1141 tccagaagat ttacatcaac ccaaggttcg tataaatgat gaacctgtga aaaaagttgt
     1201 ctttaaagtt gcgatttctt aatgtagaaa gagaagaacg atgaaaaaaa tgagaaagtt
     1261 tttatgtcta gctggaattg cgctagcggc tgttgccttg gtagcttgtt caggaaaaaa
     1321 agaagctaca actagtactg aaccaccaac agaattatct ggtgagatta caatgtggca
     1381 ctcctttact caaggacccc gtttagaaag tattcaaaaa tcagcagatg ctttcatgca
     1441 aaagcatcca aaaacgaaaa tcaagattga aacattttct tggaatgact tctatactaa
     1501 atggactaca ggtttagcaa atggaaatgt gccagatatc agtacagctc ttcctaacca
     1561 agtaatggaa atggtcaact cagatgcttt ggttccgcta aatgattcta tcaagcgtat
     1621 tggacaagat aaatttaacg aaactgcctt aaatgaagca aaaatcggag atgattacta
     1681 ctctgttcct ctttattcac atgcacaagt catgtgggtt agaacagatt tgttaaaaga
     1741 acataatatt gaggttccta aaacttggga tcaactctat gaagcttcta aaaaattgaa
     1801 agaagctgga gtttatggct tgtctgttcc gtttggaaca aatgacttaa tggcaacacg
     1861 tttcttgaac ttctacgtac gtagtggtgg aggaagcctc ttaacaaaag atcttaaagc
     1921 agacttgaca agccaacttg ctcaagatgg tattaaatac tgggttaaat tgtataaaga
     1981 aatctcacct caagattctt tgaactttaa tgtccttcaa caagctacct tgttctatca
     2041 aggaaaaaca gcatttgact ttaactctgg cttccatatc ggaggaatta atgccaacag
     2101 tcctcaattg attgattcga ttgatgctta tcctattcca aaaatcaaag agtctgataa
     2161 agaccaagga attgaaacct caaacattcc aatggttgtt tggaaaaatt caaaacatcc
     2221 agaagttgct aaagcattct tagaagcact ttataatgaa gaagactacg ttaaattcct
     2281 tgattcaact ccagtaggta tgttgccaac tattaagggg attagcgatt ctgcagccta
     2341 taaagaaaat gaaactcgta agaaatttaa acatgctgaa gaagtaatta ctgaagctgt
     2401 taaaaaaggt actgctattg gttatgaaaa tggaccaagt gtacaagctg gtatgttaac
     2461 taaccaacac attattgaac aaatgttcca agatatcatt acaaatggaa cagatcctat
     2521 gaaagcagca aaagaagcag aaaaacaatt aaacgattta tttgaggctg ttcagtagat
     2581 gtaaaagact agaaaatagg tgggctagtg agctgaaaag ctctagccca atcttgtaaa
     2641 agaagggaga aggagaatgg ttaaagaacg taatttaact cgctggatat ttgttttgcc
     2701 agctatgatt atcgtaggat tactctttgt ttatccgttt ttctcgagta ttttttatag
     2761 ctttaccaat aagcatttga ttatgcctaa ttataaattt gttggtttgg ctaactataa
     2821 agctgtgctg tcagatccca acttctttaa tgcgttcttt aattcaatta agtggaccgt
     2881 tttctcatta gttggtcaag ttttagtagg gtttgtattg gctttagctc ttcacagagt
     2941 acgccacttc aagaaattat ataggacctt attgattgtt ccttgggcat ttcctaccat
     3001 cgttattgcc ttctcttggc agtggattct aaacggggtt tatggctact tacctaatct
     3061 aatcgtaaaa ttaggtttaa tggaacatac acctgcattt ttgacagata gtacatgggc
     3121 attcctatgt ttggtgttta tcaacatttg gtttggagca ccaatgatta tggttaatgt
     3181 gctttcagct ttgcaaacag taccagaaga acaatttgag gctgctaaga tagatggtgc
     3241 ttcaagttgg caggtgttca agtttatcgt ctttccacat attaaagtgg ttgtaggact
     3301 tctagttgtt ttgagaactg tatggatctt taataacttt gacattatct acctcattac
     3361 tggtggtgga ccagccaatg ctacaacgac acttccaatt tttgcttaca acctgggttg
     3421 gggaactaaa ttgttgggtc gtgcttcagc agttacagta ttgctcttta tcttcttggt
     3481 ggcgatttgc tttatctact ttgctatcat cagtaagtgg gaaaaggagg gtagaaaata
     3541 atgaagaaga aatccagtat ttatttagat attctctcac atgtactttt agttggtgcg
     3601 accatcgttg caattttccc attggtatgg attatcatat cttctgtcaa agggaaaggg
     3661 gaattaactc agtatccaac acgattttgg cctgaacagt ttacattaga ttatttcact
     3721 catgttatca acgatttgca cttcattgat aacattcgaa acagtttaat cattgccttg
     3781 gctacaaccc tcattgcgat tattatttct gctatggcag cctatggtat tgttcgattc
     3841 tttcctaaat tgggagcaat catgtcgaga ctactcgtca ttacctacat tttcccacca
     3901 attttgttag caattcccta ttcaattgcc attgctaaag ttgggttaac aaatagttta
     3961 tttggcttga tgatggttta tctatctttt agtgttccat atgcagtttg gctcttagtt
     4021 ggatttttcc aaacagttcc aattggaatt gaagaagcgg ctagaattga tggtgcaaat
     4081 aaatttgtta cgttttataa ggttgtgcta ccgattgtag caccaggtat tgtagcaaca
     4141 gctatttata catttatcaa tgcttggaat gaattcctgt atgccttgat tttaattaac
     4201 aatacaggaa agatgacagt agcagtagcc cttcgttcac ttaatggttc agaaatacta
     4261 gactggggag atatgatggc agcgtctgtt attgtagttc ttccatcaat tattttcttc
     4321 tctatcatcc aaaataagat tgcaagtgga ttatcagaag gatctgtgaa gtagacgaaa
     4381 gaaggaaaaa aatgaataaa agaggtcttt attcaaaact aggaatttct gttgtaggca
     4441 ttagtctttt aatgggagtc cccactttga ttcatgcgaa tgaattaaac tatggtcaac
     4501 tgtccatatc tcctattttt caaggaggtt catatcaact gaacaataag agtatagata
     4561 tcagctcttt gttattagat aaattgtctg gagagagtca gacagtagta atgaaattta
     4621 aagcagataa accaaactct cttcaagctt tgtttggcct atctaatagt aaagcaggct
     4681 ttaaaaataa ttacttttca attttcatga gagattctgg tgagataggt gtagaaataa
     4741 gagacgccca aaagggaata aattatttat tttctagacc agcttcatta tggggaaagc
     4801 ataaaggaca ggcagttgaa aatacactag tatttgtatc tgattctaaa gataaaacat
     4861 acacaatgta tgttaatgga atagaagtgt tctctgaaac agttgataca tttttgccaa
     4921 tttcaaatat aaatggtata gataaggcaa cactaggagc tgttaatcgt gaaggtaagg
     4981 aacattacct cgcaaaagga agtattggtg aaatcagtct atttaacaaa gcaattagtg
     5041 atcaggaagt ttcaaatatt cccttgtcaa atccatttca gttaattttc caatcaggag
     5101 attctactca agctaactat tttagaatac cgacactata tacattaagt agtggaagag
     5161 ttctatcaag tattgatgca cgttatggtg ggactcatga ttctaaaagt aagattaata
     5221 ttgccacttc ttatagtgat gataatggga aaacgtggag tgagccaatt tttgctatga
     5281 agtttaatga ctatgaggag cagttagttt actggccacg agataataaa ttaaagaata
     5341 gtcaaattag tggaagtgct tcattcatag attcatccat tgttgaagat aaaaaatctg
     5401 ggaaaacgat attactagct gatgttatgc ctgcgggtat tggaaataat aatgcaaata
     5461 aagccgactc aggttttaaa gaaataaatg gtcattatta tttaaaacta aagaagaatg
     5521 gagataacga tttccgttat acagttagag aaaatggtgt cgtttatgat gaaacaacta
     5581 ataaacctac aaattatact ataaatgata agtatgaagt tttggaggga ggaaagtctt
     5641 taacagtcga acaatattcg gttgattttg atagtggctc tttaagagaa aggcataatg
     5701 gaaaacaggt tcctatgaat gttttctaca aagattcgtt atttaaagtg actcctacta
     5761 attatatagc aatgacaact agtcagaata gaggagagag ttgggaacaa tttaagttgt
     5821 tgcctccgtt cttaggagaa aaacataatg gaacttactt gtgtcctgga caaggtttag
     5881 cattaaaatc aagtaacaga ttgatttttg caacatatac tagtggagaa ctaacctatc
     5941 tcatttcgga tgatagtggt caaacatgga agaaatcctc agcttcaatt ccgtttaaaa
     6001 atgcaacagc agaagcacaa atggttgaac tgagagatgg tgtgattaga acattcttta
     6061 gaaccactac aggtaagata gcttatatga ctagtagaga ttctggagaa acatggtcga
     6121 aagtttcgta tattgatgga attcaacaaa cttcatatgg cacacaagta tctgcaatta
     6181 aatactctca attaattgat ggaaaagaag cagtcatttt gagtacacca aattctagaa
     6241 gtggccgtaa gggaggccaa ttagttgtcg gtttggtcaa taaagaagat gatagtattg
     6301 attggagata ccactatgat attgatttgc cttcgtatgg ttatgcctat tctgcgatta
     6361 cagaattgcc aaatcatcac ataggtgtac tgtttgaaaa atatgattcg tggtcgagaa
     6421 atgaattgca tttaagcaat gtagttcagt atatagattt ggaaattaat gatttaacaa
     6481 aataaaggag aaaaacatgg ttaaatacgg tgttgttgga gcagggtatt ttggagctga
     6541 attggctcgc tacatgcaaa agaatgatgg agcagagatt actcttctct atgatccaga
     6601 taatgcagag gcgattgcag aagaattggg agcaaaagta gcaagttcct tagatgagtt
     6661 ggtttctagc gatgaagtag attgtgttat cgtcgcaact ccaaataatc ttcataagga
     6721 accggttatt aaggctgcac agcatggtaa aaatgttttc tgtgaaaaac caattgcgct
     6781 ttcttatcaa gattgtcgcg agatggtaga tgcgtgtaaa gaaaacaatg taacctttat
     6841 ggcaggacat attatgaatt tctttaatgg tgttcatcat gcaaaagaac tcattaatca
     6901 aggagttatc ggagacgttc tatattgtca tacagctcgt aatggttggg aagaacaaca
     6961 actgtcagta tcatggaaaa aaattcgtga aaaatcaggt ggtcacttgt atcaccacat
     7021 ccatgaattg gattgcgttc aattccttat ggggggcatg cctgaaactg taaccatgac
     7081 aggtggaaat gtggcccatg aaggtgaaca tttcggtgat gaagatgata tgatttttgt
     7141 caatatggaa ttttctaata agcgttttgc cttgttagaa tggggttcag cttatcgttg
     7201 gggtgaacat tatgtcttaa tccaaggaag caaaggtgcc atccgcttag acttattcaa
     7261 ctgtaaagga actcttaagc tagatgggca agaaagctat ttcttgattc acgaatcgca
     7321 agaagaagat gatgatcgga ctcgtatcta tcatagtaca gagatggatg gagcaattgc
     7381 ttatggtaaa ccaggtaaac gtactccatt atggctatca tctgtcattg ataaagaaat
     7441 gcgctatctg catgagatta tggaaggagc tccagtatca gaagaatttg caaaactttt
     7501 gacaggtgaa gctgccctag aagcaattgc tactgcagat gcttgtaccc agtctatgtt
     7561 tgaagatcgc aaagtaaaat tgtcagaaat tgtaaaataa attttggtat tctcctatta
     7621 taggtcgact tgctcctctg aaagtacttt tagaggagct gtttgacttg gctagttttt
     7681 gaaactgaaa tctattatac tacaaactat tgaaagcgtt ttaattttaa ggtataataa
     7741 tctcatagaa ataaagaaaa ggaggaaaga ggatgccaca gattagcaaa gaagccttga
     7801 ttgagcaaat caaagatgga atcatcgttt cttgtcaggc tcttcctcat gaaccgcttt
     7861 atacagaagc gggaggggtg attcccttgc tggtcaaagc ggctgagcaa ggtggagcag
     7921 tcggtatccg agcaaacagt gttcgcgata tcaaggaaat taaggaagtc actaaacttc
     7981 caatcattgg gattatcaaa cgtgattatc cacctcagga acccttcatc acggctacta
     8041 tgaaagaagt tgatgaattg gcagaactgg acatcgaggt gattgctctg gattgtacca
     8101 agcgtgaacg ctacgatggt ttggaaattc aagagttcat tcgtcaggtt aaggagaaat
     8161 atcctaatca gcttttgatg gctgatacta gtatcttcga agaagggcta gcagctgtag
     8221 aagcaggaat tgactttgtc ggaacaacct tatcaggcta cacatcctac agtccaaaag
     8281 tagacggtcc agattttgaa ttgattaaga aactctgtga tgccggtgta gatgtcattg
     8341 cagaaggaaa aattcataca ccagaacaag ccaaacaaat ccttgaatat ggagtgcgag
     8401 gcatcgttgt tggtggcgcc attactagac caaaagagat tacagaacgc ttcgttgcta
     8461 gtcttaaata agatgtgagg gggagtttta tgtttaaagt tttacaaaaa gttggaaaag
     8521 cttttatgtt acctatagct atacttcctg cagcaggtct acttttgggg attggtggtg
     8581 cactttcaaa cccaaccacg atagcaactt atccaatact agacaatagt atttttcaat
     8641 caatattcca agtaatgagc tctgcaggag aggttgtatt cagtaatttg tcactacttc
     8701 tctgtgtggg attatgtatt ggcttagcga aacgagataa aggaaccgct gcgttagcag
     8761 gagtaactgg ttacttagtt atgactgcaa cgatcaaagc tttggtaaaa ctttttatgg
     8821 cagaaggatc tgcaattgat actggagtta ttggagcatt agttgtcgga atagttgccg
     8881 tatatttgca caaccgatat aacaatattc aattaccttc cgctttagga ttctttggag
     8941 gttcacgctt cgttcctatt gttacatcgt tctcttctat cttgattggc tttgtcttct
     9001 ttgttatttg gccacctttc caacaacttc ttgtttctac aggtggatat atttctcagg
     9061 cgggtccaat tggaactttt ctatatggat ttttaatgag actttctgga gcagtaggct
     9121 tacatcatat aatttaccct atgttttggt atactgaact tggtggtgtt gaaactgttg
     9181 caggacaaac agtggttgga gctcaaaaaa tattttttgc tcaattagcc gatccggccc
     9241 attctggatt atttacagaa ggaacaaggt tttttgcagg tcgtttctca acaatgatgt
     9301 tcggtttacc ggctgcctgt ttagcgatgt accatagtgt tcctaaaaat cgtcgtaaaa
     9361 aatacgcggg tttgtttttt ggagttgctt taacatcttt tattaccggt attacagaac
     9421 caattgaatt tatgtttcta ttcgtcagtc cggttctata tgttgttcac gcattccttg
     9481 atggtgttag cttctttatt gcagacgtct taaatatttc aataggaaac acattttcag
     9541 gaggtgtaat cgatttcact ttatttggaa ttttgcaggg gaacgctaag acgaattggg
     9601 ttcttcagat tccaattgga cttatttgga gtgttttgta ttatattatt tttagatggt
     9661 tcattactca attcaacgtt ctaacgccag gacgaggaga agaagtagat tctaaagaaa
     9721 tttctgaatc cgcagattca acttcaaata ctgcagatta tttaaaacag gatagcctac
     9781 aaattatcag agccttgggt ggatcaaata atatagaaga tgtagatgct tgtgtgacac
     9841 gtttacgtgt agctgtaaaa gaagttaatc aagttgataa agcactttta aaacaaattg
     9901 gtgcagttga tgtcttagaa gtgaagggtg gcattcaagc aatctatgga gcaaaagcaa
     9961 tcttatataa aaatagtatt aatgaaattt taggtgtaga tgattaagta cttactgact
    10021 taataaaaaa cagaggagag tgatggatga gtaggatgaa atgaaatcgc atacaagaaa
    10081 taaagaactc attatccaag ttggatacgc ttattacata ggagaataca aatgaaattt
    10141 agaaaattag cttgtacagt acttgcgggt gctgcggttc ttggtcttgc tgcttgtggc
    10201 aattctggcg gaagtaaaga tgctgccaaa tcaggtggtg acggtgccaa aacagaaatc
    10261 acttggtggg cattcccagt atttacccaa gaaaaaactg gtgacggtgt tggaacttat
    10321 gaaaaatcaa tcatcgaagc gtttgaaaaa gcaaacccag atataaaagt gaaattggaa
    10381 accatcgact tcaagtcagg tcctgaaaaa atcacaacag ccatcgaagc aggaacagct
    10441 ccagacgtac tctttgatgc accaggacgt atcatccaat acggtaaaaa cggtaaattg
    10501 gctgagttga atgacctctt cacagatgaa tttgttaaag atgtcaacaa tgaaaacatc
    10561 gtacaagcaa gtaaagctgg agacaaggct tatatgtatc cgattagttc tgccccattc
    10621 tacatggcaa tgaacaagaa aatgttagaa gatgctggag tagcaaacct tgtaaaagaa
    10681 ggttggacaa ctgatgattt tgaaaaagta ttgaaagcac ttaaagacaa gggttacaca
    10741 ccaggttcat tgttcagttc tggtcaaggg ggagaccaag gaacacgtgc ctttatctct
    10801 aacctttata gcggttctgt aacagatgaa aaagttagca aatatacaac tgatgatcct
    10861 aaattcgtca aaggtcttga aaaagcaact agctggatta aagacaattt gatcaataat
    10921 ggttcacaat ttgacggtgg ggcagatatc caaaactttg ccaatggtca aacatcttac
    10981 acaatccttt gggcaccagc tcaaaatggt atccaagcta aacttttaga agcaagtaag
    11041 gtagaagtgg tagaagtacc attcccatca gacgaaggta agccagctct tgaatacctt
    11101 gtaaacgggt ttgcagtatt caacaataaa gacgacaaga aagtcgctgc atctaagaaa
    11161 ttcatccagt ttatcgcaga tgacaaggag tggggaccta aagacgtagt tcgtacaggt
    11221 gctttcccag tccgtacttc atttggaaaa ctttatgaag acaaacgcat ggaaacaatc
    11281 agcggctgga ctcaatacta ctcaccatac tacaacacta ttgatggatt tgctgaaatg
    11341 agaacacttt ggttcccaat gttgcaatct gtatcaaatg gtgacgaaaa accagcagat
    11401 gctttgaaag ccttcactga aaaagcgaac gaaacaatca aaaaagctat gaaacaatag
    11461 tccttagtta ttctataaaa agtagttttt taaagaacct aagagtgtat accccctttt
    11521 ccctctacac agatagtgta agaaaagggg gcttttgttt aaaatgtaag aaactgtcac
    11581 gaaattaaaa tgaagttctt acataagcga atcataaaaa atttcatttt gattttaaaa
    11641 cagttcaaga aagtcaaaaa attattctat ttgaaagaga ggtgccgact gtgaaagtca
    11701 ataaaatccg tatgcgggaa acagtgattt cctacgcttt cctagcacca gtattattct
    11761 tctttgtcat ctttgtgttg gctccgatgg tgatgggctt cattacaagt ttctttaact
    11821 actcaatgac taaatttgag tttgtaggct tggataacta tatccgtatg tttaaagatc
    11881 ctgtctttac aaaatctctg attaacacag ttattttggt tattggatct gtaccagttg
    11941 ttgttctatt ctcactcttt gtagcatctc agacctatca tcaaaatgtc attgccagat
    12001 ccttctaccg tttcgtcttc ttccttcctg ttgtaacggg tagtgttgcc gtgacagttg
    12061 tttggaaatg gatttatgac ccactatcag ggattctaaa ctttgtcctt aagtccagcc
    12121 acatcatcag ccaaaacatt tcttggttgg gagataaaaa ctgggcattg atggcgatta
    12181 tgattattct cttgaccact tcagttggtc agcccatcat cctttatatc gctgccatgg
    12241 ggaatattga caattcactg gttgaagcgg cgcgtgttga tggtgcaact gagtttcaag
    12301 ttttttggaa gattaaatgg ccaagccttc ttccaacaac tctttatatt gcaatcatca
    12361 caacaattaa ctcattccag tgttttgcct tgattcagct tttgacatct ggtggtccaa
    12421 actactcaac aagtaccttg atgtactacc tttacgaaaa agccttccaa ttgacagaat
    12481 acggctatgc caacacaatt ggtgtcttct tggcagtcat gattgctatc gtaagctttg
    12541 ttcaatttaa agtacttgga aacgacgtag aatactaaag aaaggagaca gctatgcaat
    12601 ctacagaaaa aaaaccatta acagccttta ctgttatttc aacaatcatt ttgctcttgt
    12661 tgactgtgct gttcatcttt ccattctact ggattttgac aggggcattc aaatcacaac
    12721 ctgatacaat tgttattcct cctcagtggt tccctaaaat gccaaccatg gaaaacttcc
    12781 aacaactcat ggtgcagaac cctgccttgc aatggatgtg gaactcagta tttatctcat
    12841 tggtaaccat gttcttagtt tgtgcaacct catctctagc aggttatgta ttggctaaaa
    12901 aacgtttcta tggtcaacgc attctatttg ctatctttat cgctgctatg gcgcttccaa
    12961 aacaagttgt ccttgtacca ttggtacgta tcgtcaactt catgggaatc cacgatactc
    13021 tctgggcagt tatcttgcct ttgattggat ggccattcgg tgtcttcctc atgaaacaat
    13081 ttagtgaaaa tattcctaca gagttgcttg aatcagctaa aatcgacggt tgtggtgaga
    13141 ttcgtacctt ctggagtgta gccttcccga ttgtgaaacc agggtttgca gcccttgcaa
    13201 tctttacctt catcaatact tggaatgact acttcatgca attggtaatg ttgacttcac
    13261 gtaacaattt gaccatctca cttggggttg cgaccatgca ggctgaaatg gcaaccaact
    13321 atggtttgat tatggcagga gctgcccttg ctgctgttcc aatcgtcaca gtcttcctag
    13381 tcttccaaaa atccttcaca cagggtatta ctatgggagc ggtcaaagga taatactctg
    13441 agaaaatctc ttcaaactac gtcagcgtca ccttgccata cttaagtatt gcctgcggtt
    13501 agcttcctag tttgttcttc aattttcatt gagtatagga aaatcaatct atcaagatac
    13561 agaagtatat tttatagatt tagagaatat agaggttata agtgtctaca aaatggaggg
    13621 tatgcagtta ctttatgaag ttttgtcaga cacttataaa cttaagaatg gttttagtta
    13681 actatcagaa acgaaggaaa gagtatgatt tttgacgatt tgaaaaacat caccttttac
    13741 aaagggattc atcctaattt agacaaggct atcgactatc tctaccaaca tcgtaaggat
    13801 tctttcgaat taggaaagta tgatattgat ggagataaag tctttctagt tgttcaggaa
    13861 aatgtcctca atcaagctga aaatgatcaa tttgagtatc ataagaacta tgcagatttg
    13921 catttgctgg tagaaggaca tgaatattcg agctacggtt cacgtatcaa agacgaggca
    13981 gtagcattcg acgaagcgag tgacattggc tttgttcatt gtcatgaaca ctacccactc
    14041 ttgttgggtt atcacaattt tgcgattttc ttcccaggtg agccacatca gccaaatggt
    14101 tatgcaggca tggaagaaaa ggttcggaaa tatctcttta aaattttgat tgattaaaaa
    14161 taggatgaat tgtttttttg taaagctttg acaatactct accatgaaat tgatctttgt
    14221 gaggtaggga aatgagaata aaatatttaa aaattggtat cttctaagta tgctgcaaga
    14281 gctagtttct tggatggaca gggaattaca gttgatgaga tggcttggat aattaggggc
    14341 attgtgaatg gattgattgg tagatacata aaattaggta cttatgcagc caagtatggt
    14401 attagtatgg cgcgttcgat cttaagtagg gcagctgcaa ctgcagcagc aagagtagga
    14461 ttatcgacca agatttctgg atggatttta cgagtagctg tgaatgtagc tgatgtatat
    14521 ggtaattttg ccaacaatat tgctgcagct tgggatgcac atgataaaat tcctaacaat
    14581 ggtcgtataa acttttaaaa tgcgagaatg aaagcacttt gtattttttt attgaatatg
    14641 ttagcttgga cagtgcttgc aatgataatt cgtggagggc tagatggatt tgatgagcat
    14701 acttggagta ctattttaat tgcttcgctg ttcggggtat atgattataa gcccatagat
    14761 aaaaatagaa aaaagtccaa aagaaaaaag aaaaaagaaa aaatagattt gttcatggta
    14821 gggacttatg aaagctttac tgacaaaaaa gaaaacagtt tacaaagaaa aatgatggag
    14881 gagcaaacat ggcacaaaaa ggagtaagcc ttatcaaggc agcatttgat acagacaact
    14941 ttctcatgcg ttttagtgag aaagtcttgg acatcgtgac agccaatctt ctttttgtcg
    15001 tctcttgttt gcccatcgtg acgattggag tggctaaaat cagcctctac gagaccatgt
    15061 tcgaagttaa gaagagcaga cgggtgcctg tttttaaaat ctatctaaga tctttcaagc
    15121 aaaatctgaa actaggtctt cagctgggtt taatggagtt aggaattgtg tttcttaccc
    15181 tttcagatct ctatcttttc tggggtcaaa cagctctgcc cttccaattg ctgaaagcca
    15241 tttgtttagg tattctgatt tttcttacta tcgtgatgct ggctagttac cctatcgcgg
    15301 cacgttatga cctatcttgg aaagaaattc ttcaaaaagg attgatgttg gctagtttta
    15361 actttccttg gttcttcctc atgttagcca ttcttgtcct cattgtgatg gttctttatc
    15421 tgtccgcctt cagtctactc ttaggtggct cagtcttctt actttttggg tttggactat
    15481 tggtctttat ccagactgga ttgatggaga aaattttcgc aaaataccaa taggagcctt
    15541 atttctgaaa ctactttcaa acgctccaaa cgctattcta taagcgagaa actaaaatct
    15601 aagtgtctat aagatattga aagccttttc ggtaaagtgt atactaaaga agtaaagaaa
    15661 aatctgcttt agcagaaaat aaaaataata ggagaaaatc tatgtcagat ttgaaaaaat
    15721 acgaaggtgt cattccagcc ttctacgcat gttatgatga acaaggagaa gtaagcccag
    15781 aacgtacgcg tgccttggtt caatacttca ttgataaagg tgttcaaggt ctttatgtca
    15841 atggttcttc tggtgaatgt atctaccaaa gtgttgaaga ccgcaagtta atcttggaag
    15901 aagtcatggc ggtagccaaa ggtaaattga ccatcattgc tcatgttgcc tgcaacaata
    15961 ctaaagatag tatggaactt gctcgccatg ctgaaagctt gggagtagat gctattgcaa
    16021 cgattccacc aatttatttc cgcttgccag aatactcagt tgccaaatac tggaacgata
    16081 tcagttctgc agctccaaac acagactacg tgatttacaa cattcctcaa ttggcagggg
    16141 ttgctttgac tccaagcctt tacacagaaa tgttgaaaaa tcctcgtgtt atcggtgtta
    16201 agaactcttc tatgccagtt caagatatcc aaacctttgt cagccttggt ggggaagacc
    16261 atatcgtctt taatggtcct gatgagcagt tcctaggagg acgcctcatg ggtgctaggg
    16321 ctggtatcgg tggtacttat ggtgctatgc cagaactctt cttgaaactc aatcagttga
    16381 ttgcggataa ggacctagaa acagcgcgtg aattgcagta tgctatcaac gcaatcattg
    16441 gtaaactcac ttctgctcat ggaaatatgt acggtgtcat taaagaagtc ttgaaaatca
    16501 atgaaggctt gaatattgga tctgttcgtt caccattgac accagtgact gaagaagatc
    16561 gtccagttgt agaagcggct gctgccttga ttcgtgaaac caaggagcgc ttcctctaat
    16621 ctaaaaggag gtatttatga catactacgt tgcaattgat atcggtggaa ccaacatcaa
    16681 gtatggtttg gttgatcaag aggggcaact tcttgaatcg catgaaatgc caactgaggc
    16741 gcataagggt ggacctcata tcttacaaaa gaccaaagat atcgtagcta gttatttaga
    16801 aaaaggccca gtagcaggtg ttgccatatc ttctgctggg atggtggatc cggataaggg
    16861 tgagattttc tatgctgggc ctcaaatccc taactacgca ggcacccagt tcaaaaagga
    16921 aatcgaagaa agctttacta ttccttgtga gattgaaaat gatgtcaact gtgcaggtct
    16981 tgctgaggca gtatctggtt caggcaaggg agcaagtgtg acactttgct tgaccattgg
    17041 aaccggtatc ggtggttgct tgattatgga taggaaagtc ttccatggtt ttagcaattc
    17101 agcctgtgaa gtcgggtata tgcatatgca ggatggagct tttcaggatc tcgcttctac
    17161 aacagcttta gtgaaatatg tagctgaagc ccatggagaa gatgttgatc agtggaatgg
    17221 ccgacgtatc tttaaggaag ctactgaagg aaacaaaatc tgcatggaag gtattgaccg
    17281 tatggttgac tatctaggaa aaggtctggc aaatatttgt tatgtggcca atccagaagt
    17341 ggttattctt ggtggtggta tcatggggca agaggctatc ctcaaaccta agatccgcac
    17401 agccttgaaa gaggcattgg taccaagttt agcagaaaaa acacgattag aatttgccca
    17461 tcaccaaaat acagcaggga tgttgggtgc atattatcat tttaagacaa aacaatccta
    17521 gtttggctca gccaaactag gattttctta cacgtttttg tctacgatag ccgttgagtt
    17581 ttttattttc ccagtagcta ttaaagattt tttccttgct ttcgcgattg atttccaaaa
    17641 agtaggcata aatcaaatcg ataaagaaga gcataggaag ttgagcggat attcgttgga
    17701 tgtaggaggg ttggctgtgg gtggctacaa gaacagtctc tgtataggtc tggctatctt
    17761 tattgggaac acttgtaaag agtacagtct ttgcccccat ctccttggca tctaatagac
    17821 tatctaaaat agaaggagtt gagcctgaaa gtgagaaacc cagtactaga caattttcat
    17881 ccatgatgct ggttgtccag gcaaagccgt cttggtctgt caaagcttcg cagaccacac
    17941 ctagtcgcat aaaacgtaat ttcatttcac gggcgacgag gccagaactc cctgttccaa
    18001 agaagtagat acgctcagca tcttcgatta gctgggcaat tcgctctagt tggatttcgt
    18061 caatcaagtc ttgtgtttgt tccctcatat tgctataact tctgaggact cgtttggtca
    18121 gtggactatg cttggagact tggttggctt gattttctgc ctgatgttgg tattggaaaa
    18181 taaattctcg gtagccagta aagccacact ttttagcaaa gcgggtcaaa gcagcttgag
    18241 aaatatgtaa tttttgggtg acttgttgag aagataaatc atctgtaatc gtttcagctt
    18301 gcaaaaaata gcgagcgatt tcttgctcta ggtctgtcat ttcttcaaaa tgtgaatcaa
    18361 tgatagttgc gatatctggt ttgtccatag ggaaagctcc tttacatggg tcatactgga
    18421 agactagatc agggaatagt cacacttcat tataacacat aatataagga tagataaata
    18481 aaaacgcatc tctgttttaa aaacgaaaaa atcgaaaaag cttctctctt ttccataatt
    18541 ttctactcaa attgtggtac aattaagagt aagattttaa gttagaaatg agactgattt
    18601 gtatgagaaa atttaacagc cattcgattc cgattcggct taatttattg ttttcaatcg
    18661 tcattttact ctttatgacc attattggtc gtttgttgta tatgcaggtt ttgaacaagg
    18721 atttttacga aaaaaagcta gcctcagcta gtcagaccaa gattacaagc agttcagccc
    18781 gtggggaaat ttatgatgct agtggaaaac ctttggtaga aaatacgtta aagcaggttg
    18841 tttcctttac gcgtagcaat aaaatgacgg ctacagactt aaaagaaaca gctaaaaagt
    18901 tactgactta tgtgagcatc agttctccaa atttgacaga acgccagctg gcggattact
    18961 atttggctga tcctgaaatc tataaaaaaa cagtggaagc tctcccaagt gagaaacgct
    19021 tggattcaga tggcaatcgt ctatccgaat cagaactgta taacaatgcg gtcgatagtg
    19081 tccaaacaag tcaactaaac tatacagagg atgaaaagaa agaaatctat ctttttagtc
    19141 agttaaatgc tgttggaaac tttgcgacag gaaccattgc tacagatcct ctaaatgatt
    19201 ctcaggtggc tgttattgcc tctatttcaa aggagatgcc tggcattagt atttctactt
    19261 cttgggatag aaaggttttg gaaacttccc tttcttctat agttgggagt gtatccagtg
    19321 aaaaagctgg tctcccagcg gaagaagcag aagcctatct taaaaaaggc tattctctaa
    19381 atgatcgtgt aggaacctcc tatttggaaa agcaatatga agagacctta caaggaaaac
    19441 gctcggtaaa agaaatccat ctggataaat atggcaatat ggaaagcgtg gatacaattg
    19501 aggaaggtag taagggaaac aatatcaaac tgaccattga tttggctttc caagatagcg
    19561 tggatgcttt actgaaaagt tatttcaatt ccgagctaga aaatggtgga gccaagtatt
    19621 ctgaaggtgt ctatgcagtc gcccttaacc caaaaacagg tgcggttttg tctatgtcag
    19681 ggattaaaca tgacttgaaa acgggagagt tgacgcctga ttccttggga acggtaacca
    19741 atgtctttgt tccaggttcg gttgtcaagg cggcgaccat cagctcaggt tgggaaaatg
    19801 gagtcttgtc aggaaaccag accttgacag accagtccat tgtcttccaa ggttcagctc
    19861 ccatcaattc ttggtatact caggcttacg gttcattccc tatcacagcg gtccaagctc
    19921 tggagtattc atcaaatacc tatatggtcc aaacagcctt aggtcttatg gggcaaacct
    19981 atcaacccaa tatgtttgtc ggcaccagca atctagagtc tgctatggag aaactgcgtt
    20041 caacctttgg cgaatatggc ttgggtactg cgacaggaat tgacctacca gatgaatcta
    20101 ctggatttgt tcccaaagag tatagctttg ctaattacat tactaatgcc tttgggcagt
    20161 ttgataacta tacgccgatg cagttggctc agtatgtagc aactattgca aataatggtg
    20221 ttcgtgtggc tcctcgtatt gttgaaggca tttatggtaa taatgataag ggaggactgg
    20281 gtgacttgat tcagcaactg caaccgacag agatgaataa ggtcaatata tccgactccg
    20341 atatgagcat cttgcaccaa ggtttttatc aggttgccca tggtactagt ggattgacaa
    20401 ctggacgtgc cttttcaaat ggcgccttgg tatccattag cggaaaaaca ggtacagccg
    20461 aaagctatgt ggcagatggt cagcaagcaa ccaataccaa tgcggtggcc tatgccccat
    20521 ctgataatcc ccaaatcgct gttgcagtgg tctttcctca taataccaat ctaacaaatg
    20581 gtgtaggacc ttccattgcg cgtgacatta tcaatctgta tcaaaaatac catccaatga
    20641 actagaaagg aaattatgct ttatccaaca cctattgcca agttgattga cagttattct
    20701 aagttaccag gtatcgggat taagacggct acgcgtctgg ccttttatac gattgggatg
    20761 tctgctgatg atgtcaatga atttgcaaaa aatctccttt ctgctaagag agaattgaca
    20821 tattgttcta tttgtggacg tttgacagac gacgatcctt gttctatctg tactgatccg
    20881 actcgtgacc agacaacaat tttagttctt gaggatagta gagatgtggc agccatggaa
    20941 aatatccaag aataccatgg actctatcat gtccttcatg gcctcatttc tcctatgaat
    21001 ggtatcagtc cggacgatat caatctcaag agccttatga ctcgtcttat ggatagtgaa
    21061 gtttcagaag tgattgtggc gactaatgct acagcggatg gtgaagcgac ttccatgtat
    21121 ctttcacgtt tgctcaagcc ggctggtatc aaggttacgc gtctagcacg aggtctcgct
    21181 gtgggagcgg acattgagta tgcggacgaa gtgacactct tacgagcgat tgaaaatcgg
    21241 acagagttgt aagtgtaggc aaatttacga actccattca tttataaaaa atcaaagagg
    21301 ctgaaaatcg ttcctatcgg cctctttttg tatagtgtga tgagtaggat caggttcaag
    21361 ttttaaaaaa ccaagcaaat atgatatact aaagagcgag tattctagta gaattaggac
    21421 aaataatatg aaacaaacga ttattctttt atatggtgga cggagtgcgg aacgcgaagt
    21481 ctctgtcctt tcagctgaga gtgtcatgcg tgcggtcaat tacgaccgtt tcacagtcaa
    21541 gactttcttt atcagtcagt caggtgactt tatcaaaaca caggaattta gtcatgctcc
    21601 ggggcaagaa gaccgtctca tgaccaatga aaccattgat tgggataaga aagttgcacc
    21661 aagtgctatc tacgaagaag gtgcagtggt ctttccagtc cttcacgggc caatgggaga
    21721 agatggctct gttcaaggat tcttggaagt tttgaaaatg ccttacgttg gttgcaacat
    21781 tttgtcatca agtcttgcca tggataaaat cacgactaag cgtgttctgg aatctgctgg
    21841 tattgcccaa gttccttatg tggctatcgt tgaaggcgat gatgtgactg ctaaaatcgc
    21901 tgaagtggaa gaaaaattgg cttatccagt cttcactaag ccgtcaaaca tggggtctag
    21961 tgtcggtatt tctaagtctg aaaaccaaga agaactccgt caagccttaa aacttgcctt
    22021 ccgatatgac agccgtgtct tggttgagca aggagtgaat gcccgtgaaa ttgaggttgg
    22081 cctcttgggt aactacgatg tcaagagcac gctacctgga gaagttgtca aggacgttgc
    22141 cttttatgac tacgatgcca agtatattga taacaagatt actatggata ttcctgccaa
    22201 aatcagtgat gatgtggtgg ctgtcatgcg tcaaaatgca gaaacagcct tccgtgccat
    22261 tggtggcctt ggtctatctc gttgcgattt cttctataca gataagggag agatttttct
    22321 caacgagctc aataccatgc caggttttac ccagtggtct atgtacccac tactttggga
    22381 caatatgggg atcagctacc cagaactaat cgagcgtttg gttgaccttg ccaaggaaag
    22441 ttttgacaag cgcgaagcgc atttgatata aaatgaaaga gagggtagaa gccagaacca
    22501 tcactgcacg gtgactagag ttctcggact tcagcccttt ttaaaggagt agaaatgaaa
    22561 ttaacaatcc atgaaattgc ccaagttgtt ggagccaaaa atgatatcag tatctttgag
    22621 gacacccagt tagaaaaagc tgagtttgat agtcgtttga ttggaactgg agatttattt
    22681 gtgccactta aaggtgcgcg tgatggccat gactttattg aaacagcctt tgaaaatggt
    22741 gcagcagtaa ccttgtctga gaaagaggtc tcaaatcatc cttacattct agtagatgat
    22801 gttttgacag cctttcaatc cctagcatcc tactatcttg aaaaaacgac tgttgatgtc
    22861 tttgctgtta caggttcaaa tggcaagaca acgactaagg atatgttggc gcatttactg
    22921 tcaacaagat acaagaccta caaaacacaa ggcaattaca ataatgagat tggccttcct
    22981 tacacagttc ttcatatgcc tgaaggaaca gaaaagttgg ttttggagat gggacaggat
    23041 cacttgggag atattcatct cttgtctgaa ttggctcgtc caaaaacagc catcgtgacc
    23101 ttggttggag aagctcattt ggcctttttc aaagatcgtt ctgagattgc taaaggaaaa
    23161 atgcaaattg cagacggaat ggcttcaggt tccttgcttt tagcgccggc tgaccctatc
    23221 gtagaggact atttgccaac tgataaaaag gtggttcgtt ttggtcaagg agcagagctg
    23281 ggaattaccg acttggttga gcgcaaagat agtctgacct tcaaggtcaa tttcttagag
    23341 caagcccttg atttgccagt aactggcaag tacaatgcga caaatgctat gattgcatcc
    23401 tatgttgcct tgcaagaagg agtttcagag gagcaaattc gtttggcctt ccaacatctt
    23461 gaattgacgc gtaaccgtac cgagtggaag aaagcagcca atggagcaga tatcctgtca
    23521 gatgtttata atgccaatcc aactgctatg aaactgattt tagagacttt ctctgccatt
    23581 ccagccaatg aaggtggcaa gaaaattgca gtgttggcgg atatgaagga gcttggtgac
    23641 cagtctgttc aacttcataa tcagatgatt ttgagcctct ccccagatgt gcttgatatc
    23701 gttattttct atggagaaga tattgctcag ttggctcaat tggccagtca aatgtttcca
    23761 atcggccacg tttactactt caagaaaaca gaagaccagg atcaatttga agacctagtc
    23821 aagcaggtta aggaaagcct tggagcctat gaccaaatct tgctaaaagg ttctaactct
    23881 atgaatctag ccaaattggt agaacgttta gaaaatgaag ccaagtgatt ttgtcaagta
    23941 tctgcaaaga atgattgccc ttacagatac tggcttaacc tttacaaaag atccttttga
    24001 ccgtgagcgc tacgaggact tgcgaagtct gttatctgaa atgttgaatc agggatcaga
    24061 cctggatgca gaagaagtag cagaagtctt gaaaccaact tcagcttatg cgactccctt
    24121 aatggacgtc cgtgcttgga ttgttgagga tgagaagatt tgtctggtta ggggacaagg
    24181 agaggatagt tgggctttgc caggtggctt tggtgaagtc ggctattctc caactgaaaa
    24241 tattctcaag gaaattgaag aagaaaccgg ttttaaagcc aaagttgaaa gactgctagc
    24301 tgtttttgat accaatcgtt tccaactaca gagtaaacaa tatgcaaagt ttgtttttga
    24361 atgtaagttt ctcgatggac aattccaaga aaatcaagaa attgcagacc ttcaattttt
    24421 tgccattgat caactgccag ccttatctga aaaacgcatt accaaggagc aaatcgagat
    24481 tctttggcag gtttatcaag gtcagaggga gcaatatctt gactaagaag atgattatcg
    24541 tatttttaaa tccattttta acaactagca tggtataata atatgcagga aaattttgaa
    24601 ttatgaggaa gactagatga atttatggga tattttcttt acgactcagg caaccgagcc
    24661 gcccaaattt gacctttttt ggtatgttag cctatttacg ctcttagcct taacctttta
    24721 tacagcccat cgctatcgtg aaaagaaggt ttaccaacga tttttccaaa tcttgcagac
    24781 tgttcagtta atccttcttt atggttggta ctgggtcaat catatgccac tgtcagaaag
    24841 cctacccttt taccattgcc gtatggctat gtttgtggta ctcttgcttc ctggtcagtc
    24901 caaatataaa caatactttg cattattggg aacatttggg acattagcag cctttgttta
    24961 tccagtgcca gatgcttacc cttttccaca tatcaccatt ctatccttta tctttggtca
    25021 tttagcactc ttggggaact ctctagttta tctattgaga cagtataatg cgcgattgct
    25081 ggatgtgaag ggaatttttc tcatgacctt tgccctaaat gccttgattt ttgtggtcaa
    25141 tttggtgaca ggtggcgatt acggattttt gacaaaaccg ccattggttg gggatcacgg
    25201 tctagtagct aattatttac ttgtttcaat tgtgctggta gctactatca gtttgactaa
    25261 gaaaatctta gaattctttt tagctcaaga agcagaaaaa atgattgcaa aggaagctta
    25321 acacagagct ttcttttttg ctcttagaga gtttttacaa gcagcttata aaataagaat
    25381 ttctgaatag acaaactcaa aaaatggctg ggaaatttag gaaaaaagca agcacgatta
    25441 aattttttgt gttataatat tttgtgaata gctatgccta tgtttagcta tggaataata
    25501 cgaagtgcga aacttggaag atagagagga agcgatgtaa tggctagaga aggctttttt
    25561 acaggtctag atattggaac aagctctgtc aaggtgcttg tggccgagca gagaaatggt
    25621 gaattaaatg taattggcgt gagtaatgcc aaaagtaaag gtgtaaagga tggaattatt
    25681 gttgatattg atgcagcagc aactgctatc aagtcagcca tttcccaagc ggaagaaaag
    25741 gcaggcattt cgattaaatc agtgaatgtc ggcttgcctg gtaatctttt gcaggtagaa
    25801 ccaactcagg ggatgattcc agtaacatct gatactaagg aaattacgga tcaagatgtt
    25861 gaaaatgttg tcaaatcagc tttgacaaag agtatgacac ctgaccgtga agtcattacc
    25921 tttattcctg aagaatttat tgtggatggt ttccaaggga ttcgtgaccc acgtggtatg
    25981 atgggggttc gccttgaaat gcgtggtttg ctttatacag gacctcgtac tatcttgcac
    26041 aatttgcgta agacggttga gcgtgcaggt gttcaggttg aaaatgttat catttcacca
    26101 ctagcaatgg ttcagtctgt tttgaacgaa ggggaacgtg aatttggtgc tacagtgatt
    26161 gatatggggg caggtcaaac gactgtcgct acaatccgta atcaagaact ccagttcaca
    26221 catattctcc aagaaggtgg agattatgta actaaagata tctccaaggt tttgaaaacc
    26281 tctcgcaaat tagcggaagg cttgaaactg aattacgggg aagcctatcc gcctcttgca
    26341 agcaaagaaa ccttccaagt agaggttatt ggagaagtgg aagcagtcga agtgacggaa
    26401 gcctacttgt cagaaattat ttctgcacga atcaagcaca tccttgaaca aatcaagcaa
    26461 gaattagata gaaggcgtct attggacctc cctggtggta ttgtcttaat cggtgggaat
    26521 gccattttac caggtatggt tgagcttgct caggaagtct ttggcgtccg tgtcaagctt
    26581 tatgttccaa atcaagttgg tatccgtaat ccagcctttg cgcatgtgat tagtttatca
    26641 gaatttgcgg gtcaattaac agaagttaat cttttggctc agggagcgat aaaaggtgag
    26701 aatgacttaa gtcatcagcc aattagtttt ggtgggatgc tgcaaaaaac agctcagttt
    26761 gtacaatcaa cgcctgttca accagctcct gctccagaag tagagccggt ggcgcctaca
    26821 gaaccaatgg cggatttcca acaagcttca caaaataaac cgaaattagc agatcgtttc
    26881 cgtggcttga tcggaagcat gtttgacgaa taaagaggaa aaataaatta tgacattttc
    26941 atttgataca gctgctgctc aaggggcagt gattaaagta attggtgtcg gtggaggtgg
    27001 tggcaatgcc atcaaccgta tggtcgacga aggtgttaca ggcgtagaat ttatcgcagc
    27061 aaacacagat gtacaagcat tgagtagtac aaaagctgag actgttattc agttgggacc
    27121 taaattgact cgtggtttgg gtgcaggagg tcaacctgag gttggtcgta aagccgctga
    27181 agaaagcgaa gaaacactga cggaagctat tagtggtgcc gatatggtct tcatcactgc
    27241 tggtatggga ggaggctctg gaactggagc tgctcctgtt attgctcgta tcgccaaaga
    27301 tttaggtgcg cttacagttg gtgttgtaac acgtcccttt ggttttgaag gaagtaagcg
    27361 tggacaattt gctgtagaag gaatcaatca acttcgtgag catgtagaca ctctattgat
    27421 tatctcaaac aacaatttgc ttgaaattgt tgataagaaa acaccgcttt tggaggctct
    27481 tagcgaagcg gataacgttc ttcgtcaagg tgttcaaggg attaccgatt tgattaccaa
    27541 tccaggattg attaaccttg actttgccga tgtgaaaacg gtaatggcaa acaaagggaa
    27601 tgctcttatg ggtattggta tcggtagtgg agaagaacgt gtggtagaag cggcacgtaa
    27661 ggcaatctat tcaccacttc ttgaaacaac tattgacggt gctgaggatg ttatcgtcaa
    27721 cgttactggt ggtcttgact taaccttgat tgaggcagaa gaggcttcac aaattgtgaa
    27781 ccaggcagca ggtcaaggag tgaacatctg gctcggtact tcaattgatg aaagtatgcg
    27841 tgatgaaatt cgtgtaacag ttgtcgcaac gggtgttcgt caagaccgcg tagaaaaggt
    27901 tgtggctcca caagctagaa ctgctactaa ctaccgtgag acagtgaaac cagctcattc
    27961 acatggcttt gatcgtcatt ttgatatggc agaaacagtt gaattgccaa aacaaaatcc
    28021 acgtcgtttg gaaccaactc aggcatctgc ttttggtgat tgggatcttc gccgtgaatc
    28081 gattgttcgt acaacagatt cagtcgtttc tccagtcgag cgctttgaag ccccaatttc
    28141 acaagatgaa gatgaattgg atacacctcc atttttcaaa aatcgttaag taaatgaatg
    28201 taaaagaaaa tacagaactt gtttttcgag aagttgcaga ggctagtctg agtgctcatc
    28261 gagagagtgg ttcggtctct gtcattgcag ttaccaagta tgtagatgta ccgacagcgg
    28321 aagccttgct tccgctaggt gttcatcata tcggtgaaaa tcgtgtagat aagtttctgg
    28381 aaaaatatga agctttaaaa gatcgagatg tgacttggca tttgattggt accttgcaaa
    28441 gacgtaaggt gaaagatatc attcaatacg ttgattattt ccatgcattg gactcagtaa
    28501 agctagcagg ggaaattcaa aaaagaagtg accgagtcat caagtgtttc cttcaagtaa
    28561 atatttctaa agaagaaagc aaacacggtt tttcgagaga ggaactgctg gaaatcttgc
    28621 cagagttagc cagactagat aagattgaat atgttggttt aatgacgatg gcaccttttg
    28681 aggctagcag tgagcagttg aaagagattt tcaaggcgac ccaagattta caaagagaaa
    28741 ttcaagagaa acaaattcca aatatgccta tgaccgagtt aagtatggga atgagtcgtg
    28801 attataaaga agcgattcaa ttcggttcca cttttgttcg tataggtaca tcatttttta
    28861 agtaggagag aaccatgtct ttaaaagata gattcgatag atttatagat tattttacgg
    28921 aggatgagga ttcaagtctc ccttatgaaa aaagagatga gcctgtgttt actccagtaa
    28981 attcttcaca ggaaccggct ctcccaatga atcaaccttc acagtcggct ggtacaaaag
    29041 agaacaatat caccagactt catgcaagac aacaggaatt ggcaaatcag agtcagcgtg
    29101 caacggataa ggtcattata gatgttcgtt atcctagaaa atatgaggat gcaacagaaa
    29161 ttgttgattt attggcagga aacgaaagta tcttgattga ttttcagtat atgacagagg
    29221 tgcaggctcg tcgttgtttg gactatttgg atggagcttg tcatgtttta gctggaaatt
    29281 tgaaaaaggt agcttctacc atgtatttgt tgacaccagt gaatgttatt gtaaatgttg
    29341 aagatatccg tttaccagat gaagatcaac agggtgagtt cggttttgat atgaagcgaa
    29401 atagagtacg ataatgattt ttttaattcg tatgatttat aatgcagtgg atatttactc
    29461 cctgattttg gtagccttcg ctgtcatgtc ttggtttcca ggtgcctacg aatccagttt
    29521 aggtcgttgg attgtagcgt tggtgaaacc agtgcttgct cccttgcaac gcctgccttt
    29581 acagatagcg ggtcttgatt tatctgtttg ggtcgcgatt gttttggttc gatttttagg
    29641 agaaaaccta gtgcgttttc tggcgatgat aggatgaata aagggattta tcagcatttc
    29701 tccatagaag atcgtccatt tcttgacaag ggaatggaat ggataaagaa ggtagaagat
    29761 agctatgctc cttttttaac tccttttatc aatcctcatc aggagaagct attaaagatt
    29821 ttggccaaaa cctatggtct tgcttgtagc agtagtgggg aattcgtctc gagtgagtat
    29881 gttcgagttt tattataccc agattatttc caaccagagt tttcagattt tgaaatatct
    29941 ctccaggaaa ttgtgtattc caataaattt gaacatttaa cgcatgctaa gattttaggg
    30001 acagtcatca atcaattagg gattgaacgg aaactttttg gagatatcct agtagatgaa
    30061 gaacgggcgc agattatgat taatcagcag tttcttcttc tctttcaaga tggactaaag
    30121 aaaattggtc gtatacctgt ttcgctggag gaacgtcctt tcaccgagaa aatagataag
    30181 ctagaacagt atcgagaact ggatttatct gtgtctagtt ttcgattaga tgttctttta
    30241 tcaaatgttt tgaaactatc taggaatcaa gcaaaccagt tgattgaaaa gaaacttgtc
    30301 caagtaaatt atcatgtggt agacaaatca gattacactg ttcaagttgg agacttgatt
    30361 agtgtgagaa aatttggtcg cttgagatta cttcaagata agggacaaac gaaaaaagag
    30421 aagaaaaaaa taaccgtcca gttattatta agtaagtgag gaatagaatg ccaattacat
    30481 cattagaaat aaaggacaag acttttggaa ctcgattcag aggttttgat ccagaagaag
    30541 tcgatgaatt tttagatatt gtggttcgtg attacgaaga tcttgtgcgt gcgaatcatg
    30601 ataaaaattt gcgtattaag agtttagaag agcgtttgtc ttactttgat gaaataaaag
    30661 attcattgag ccagtctgta ttgattgctc aggatacagc tgagagagtg aaacaggcgg
    30721 cgcatgaacg ttcaaacaat atcattcatc aagcagagca agatgcgcaa cgcttgttgg
    30781 aagaagctaa atataaggca aacgagattc ttcgtcaagc aactgataat gctaagaaag
    30841 tcgctgttga aacagaagaa ttgaagaaca agagccgtgt cttccaccaa cgtctcaaat
    30901 ctacaattga gagtcagttg gctattgttg aatcttcaga ttgggaagat attctccgtc
    30961 caacagctac ttatcttcaa accagtgatg aagcctttaa agaagtggtt agcgaagtac
    31021 ttggagaacc gattccagct ccaattgaag aagaaccaat tgatatgaca cgtcagttct
    31081 ctcaagcaga aatggaagaa ttacaagctc gtattgaggt agccgataaa gaattgtctg
    31141 aatttgaagc tcagattaaa caggaagtgg aaactccaac tcctgtagtg agtcctcaag
    31201 ttgaagaaga gcctctgctc atccagttgg cccaatgtat gaagaaccag aagtagctcc
    31261 agtgcatccg acaggtccaa caccagctac agaaactgtt gattcagcac caggatttga
    31321 agcaccgcaa gaatccgtta caattttata agaaacgatg tgagaacaat atcttatcct
    31381 tatatttcca gcgagcagga gatggtgtga gtcctgcatt ccctaacgat aagattatcc
    31441 tctcaaaaac tcaagtctga atctagtaag atttgacgtt tcccacgtta cgggataaga
    31501 gggagaaaga ctaaatcttt ttccgaataa aggtggtacc acgattttcg tcctttttgg
    31561 aagtcgtggt ttttaatttg ttattattta taaaggagat accatgaaac taaaagatac
    31621 ccttaatctt gggaaaacag ctttcccaat gcgtgcaggc cttccgacca aagagccagt
    31681 ttggcaaaag gaatgggaag atgcaaaact ttaccaacgt cgtcaagaac taaatgaagg
    31741 gaaaccgcat tttgtcttgc atgatggccc tccatacgca aacggaaata tccacgttgg
    31801 acatgccatg aatcatatct caaaagatat catcattcgt tctaagtcta tgtcaggttt
    31861 taacgcacct tatatcccag gttgggatac tcatggtctg ccaatcgagc aagttttggc
    31921 aaagcaaggt gtgaagcgca aagaaatgga cttggttgag tacttgaaac tttgccgtga
    31981 atacgctctt tctcaagttt ataaacaacg tgatgatttc aagcgcttgg ggatgtcagg
    32041 tgactgggaa aatttatatg tgactttaac tccagactac gaagcagccc aaatccgtgt
    32101 atttggtgag atggctaata agggttatat ctaccgtggt gctaagccag tttactggtc
    32161 ttggtcatct gagtcagccc ttgccgaagc ggaaattgaa taccatgatt tggtttcaac
    32221 ttccctttac tatgccaata aggtaaaaga tggcaaaggt gttctagata cagatactta
    32281 tatcgttgtt tggacaacta ctccatttac tatcacagct tctcgtggtt tgactgttgg
    32341 tgcagatatt gattacgttt tggttcaacc tgctggtgaa actcgtaagt ttgtcgttgc
    32401 tgcagaattg ttgactagct tgtctgagaa atttggctgg gttgatgttc aagttttggc
    32461 aacttaccgt ggtcaagaac tcaaccacat cgtaacagaa catccatggg atacagctgt
    32521 agatgaattg gttatccttg gtgaccacgt tacaactgac tctggtacag gtattgtcca
    32581 tacagcccct ggttttggtg aggacgatta caatgttggt attgctaata atcttgaagt
    32641 cgcagtgact gttgatgaac gtggtatcat gatgaagaat gctggtcctg aatttgaagg
    32701 tcaattctat gaaaaggtag ttccaactgt tattgaaaaa cttggtaacc tccttcttgc
    32761 ccaagaagaa atctctcact catatccatt tgactggcgt accaagaaac caattatctg
    32821 gcgtgcagta ccacagtggt tcgcctctgt ttcaaaattc cgccaagaaa tcttggatgc
    32881 tattgataaa gttaaattcc atacagaatg gggtaaagta cgtctatata atatgattcg
    32941 tgaccgtggt gactgggtta tctctcgtca acgtgcttgg ggtgttccac ttccaatctt
    33001 ctatgcagaa gacggtacag ctatcatgac agctgagact atcgaatatg tggctcaact
    33061 ctttgaagta catggttcaa gcatttggtg ggaacgtgat gccaaagacc tcttgccaga
    33121 aggatttaca catccaggtt ctccaaatgg cgagttcaaa aaagaaactg atatcatgga
    33181 cgtttggttt gactcaggtt catcatggaa tggtgttttg gtaaatcgtc caaatctgac
    33241 ttatccagct gacctttacc ttgaaggttc tgaccaatac cgtggttggt tcaactcatc
    33301 actcattaca tctgttgcca accatggcgt agcaccttac aaacaaatct tgtcacaagg
    33361 ttttaccctt gacggtaaag gtgagaagat gtctaaatct cttggaaata ccattgctcc
    33421 aagcgatgtt gaaaaacaat ttggtgctga aatcttgcgt ctctgggtaa caagtgttga
    33481 ctcaagcaat gacgtgcgta tctctatgga tatcttgagc caagtctctg aaacttaccg
    33541 taagattcgt aatactcttc gtttcttgat tgccaataca tctgacttta acccaactca
    33601 agatgcagtc gcttacgatg aacttcgttc agttgataag tacatgacca ttcgctttaa
    33661 ccagcttgtc aagaccattc gtgatgccta tgcaaacttt gaattcttga caatctacaa
    33721 ggccttggtg aactttatca acgttgattt atcagccttt taccttgatt ttgctaaaga
    33781 tgttgtttac atcgaaggtg ctaaatcact ggaacgtcgt caaatgcaga ctgtcttcta
    33841 tgatattctt gtcaaaatca ccaaactctt gacaccaatc cttcctcaca ctgcggaaga
    33901 aatctggtca tatcttgagt ttgaaacaga agacttcgtc caattgtcag aattaccaga
    33961 agttcaaact tttgctaacc aagaagaaat cttggataca tgggcagcct tcatggactt
    34021 tcgtggacaa gcacaaaaag ccttggaaga agctcgtaat gcaaaagtta tcggtaaatc
    34081 acttgaagca cacttgacag tttatccaaa tgaagtggtg aaaactctac tcgaagcagt
    34141 aaatagcaat gtggctcaac ttttgatcgt gtctgagttg accatcgcag aaggaccagc
    34201 tccggaagct gcccttagct tcgaagatgt agccttcaca gttgaacgtg ctgctggtga
    34261 agtatgtgac cgttgccgtc gtattgaccc aacaacagca gaacgtagct accaggcagt
    34321 tatctgtgac cactgtgcaa gcatcgtaga agaaaacttt gcggaagcag tcgcagaagg
    34381 atttgaagag aaataagatt gaaaagtcta ggcaaaattc aatttgagaa gaaaagacaa
    34441 ctaattttat agtctattaa acgcattgta tcacgttttt gaacacctga tatgatgcgt
    34501 tttttattta ttttaaaaat ttgcgaggta tgacttttta tactcaacaa gaatcaaaga
    34561 gaaacttagc aagctaacag tagtaagata aaatagga
//
LOCUS       DAVKMU010000018        33947 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKMU010000018 DAVKMU010000000
VERSION     DAVKMU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.018"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(134..496)
                     /locus_tag="VW343_001013"
     CDS             complement(134..496)
                     /locus_tag="VW343_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9755580.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFGNE"
     gene            504..584
                     /locus_tag="VW343_001014"
                     /pseudo
     CDS             504..584
                     /locus_tag="VW343_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(991..1149)
                     /locus_tag="VW343_001015"
                     /pseudo
     CDS             complement(991..1149)
                     /locus_tag="VW343_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(1422..2111)
                     /gene="rplA"
                     /locus_tag="VW343_001016"
     CDS             complement(1422..2111)
                     /gene="rplA"
                     /locus_tag="VW343_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9755581.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2319..2744)
                     /gene="rplK"
                     /locus_tag="VW343_001017"
     CDS             complement(2319..2744)
                     /gene="rplK"
                     /locus_tag="VW343_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9755582.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2876..3013
                     /locus_tag="VW343_001018"
     CDS             2876..3013
                     /locus_tag="VW343_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755583.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(3053..3769)
                     /locus_tag="VW343_001019"
     CDS             complement(3053..3769)
                     /locus_tag="VW343_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9755584.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3772..4242)
                     /locus_tag="VW343_001020"
     CDS             complement(3772..4242)
                     /locus_tag="VW343_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9755585.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVVGDHQYFEGYSLFLA
                     KEHVSELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            complement(4244..5281)
                     /locus_tag="VW343_001021"
     CDS             complement(4244..5281)
                     /locus_tag="VW343_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9755586.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTSK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTI
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTSEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYKEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSTLVD"
     gene            complement(5485..6810)
                     /gene="brnQ"
                     /locus_tag="VW343_001022"
     CDS             complement(5485..6810)
                     /gene="brnQ"
                     /locus_tag="VW343_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9755587.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6981..7073)
                     /locus_tag="VW343_001023"
     CDS             complement(6981..7073)
                     /locus_tag="VW343_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9755588.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(7116..7268)
                     /locus_tag="VW343_001024"
     CDS             complement(7116..7268)
                     /locus_tag="VW343_001024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9755589.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LTDSYD"
     gene            complement(7428..8006)
                     /locus_tag="VW343_001025"
     CDS             complement(7428..8006)
                     /locus_tag="VW343_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9755590.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(8060..9460)
                     /gene="pepV"
                     /locus_tag="VW343_001026"
     CDS             complement(8060..9460)
                     /gene="pepV"
                     /locus_tag="VW343_001026"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9755591.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9473..10078)
                     /locus_tag="VW343_001027"
     CDS             complement(9473..10078)
                     /locus_tag="VW343_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9755592.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVCEKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            10249..11049
                     /locus_tag="VW343_001028"
     CDS             10249..11049
                     /locus_tag="VW343_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9755593.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKESSSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTYLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSN
                     YIVFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(11227..12060)
                     /locus_tag="VW343_001029"
     CDS             complement(11227..12060)
                     /locus_tag="VW343_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9755594.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLQGDIFLPGP
                     GANDLVALLKDLLITASVRGNWDDRVLEVLDGEYGLEYPKEIQSMRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSKGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQRHNLKLLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(12050..13894)
                     /gene="uvrC"
                     /locus_tag="VW343_001030"
     CDS             complement(12050..13894)
                     /gene="uvrC"
                     /locus_tag="VW343_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9755595.1"
                     /translation="MNNLIKPKLELLPTSPGCYIYKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNEHYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKNDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVTAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13933..14634)
                     /locus_tag="VW343_001031"
     CDS             complement(13933..14634)
                     /locus_tag="VW343_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9755596.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETNE"
     gene            complement(14713..15945)
                     /gene="murN"
                     /locus_tag="VW343_001032"
     CDS             complement(14713..15945)
                     /gene="murN"
                     /locus_tag="VW343_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9755597.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQNKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15950..17170)
                     /gene="murM"
                     /locus_tag="VW343_001033"
     CDS             complement(15950..17170)
                     /gene="murM"
                     /locus_tag="VW343_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9755598.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17282..18061)
                     /locus_tag="VW343_001034"
     CDS             complement(17282..18061)
                     /locus_tag="VW343_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9755599.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(18130..19791)
                     /locus_tag="VW343_001035"
     CDS             complement(18130..19791)
                     /locus_tag="VW343_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9755600.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19966..22188)
                     /gene="recJ"
                     /locus_tag="VW343_001036"
     CDS             complement(19966..22188)
                     /gene="recJ"
                     /locus_tag="VW343_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9755601.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22465..23223
                     /locus_tag="VW343_001037"
     CDS             22465..23223
                     /locus_tag="VW343_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9755602.1"
                     /translation="MVLVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDRMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            23263..24027
                     /locus_tag="VW343_001038"
     CDS             23263..24027
                     /locus_tag="VW343_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9755603.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            24040..24717
                     /locus_tag="VW343_001039"
     CDS             24040..24717
                     /locus_tag="VW343_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9755604.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGATLLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24727..25386
                     /locus_tag="VW343_001040"
     CDS             24727..25386
                     /locus_tag="VW343_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9755605.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25644..26834
                     /locus_tag="VW343_001041"
     CDS             25644..26834
                     /locus_tag="VW343_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9755606.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(27155..28036)
                     /locus_tag="VW343_001042"
     CDS             complement(27155..28036)
                     /locus_tag="VW343_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9755607.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(28175..29503)
                     /gene="vncS"
                     /locus_tag="VW343_001043"
     CDS             complement(28175..29503)
                     /gene="vncS"
                     /locus_tag="VW343_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9755608.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(29500..30156)
                     /gene="vncR"
                     /locus_tag="VW343_001044"
     CDS             complement(29500..30156)
                     /gene="vncR"
                     /locus_tag="VW343_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9755609.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(30205..30288)
                     /gene="pep27"
                     /locus_tag="VW343_001045"
     CDS             complement(30205..30288)
                     /gene="pep27"
                     /locus_tag="VW343_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9755610.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(30468..31847)
                     /gene="vex3"
                     /locus_tag="VW343_001046"
     CDS             complement(30468..31847)
                     /gene="vex3"
                     /locus_tag="VW343_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9755611.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31899..32546)
                     /gene="vex2"
                     /locus_tag="VW343_001047"
     CDS             complement(31899..32546)
                     /gene="vex2"
                     /locus_tag="VW343_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9755612.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32559..33836)
                     /locus_tag="VW343_001048"
     CDS             complement(32559..33836)
                     /locus_tag="VW343_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755613.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9651 a         7712 c         6347 g        10237 t
ORIGIN      
        1 ataaactaac ctcctcatta ttagcaagga ttctactgat tataacctaa ttcttagact
       61 acttcaagac taactgtcca ataataatct acagataaat catccactct taataaaatt
      121 ttattcaatc catctactca ttcccaaaag gttgagattt atctagtaac tctaaaacat
      181 taatggtagg tagcaacatt gcttcactgc tatcaattgc cgaaactatt gaaacaatag
      241 aacgtaaata atgataaaga atagcagtac catttacgta tagaatatcg gtgatatttt
      301 catctatatt tatttcaaaa tatcctatta cagttacaga tatttttttc atggcatctt
      361 tgtcaactaa agaaacacta agttttacgg ctccatgact ataatcatct gtatgaccaa
      421 ccttaactat gggatataat atatcgaagg taaatgtgaa caaactgatt aggaaattta
      481 aaccaacttc taacaatctg ttatactcaa tgaaaaccaa agagcaaact aggaaactag
      541 cctcaggttg ctcaaaacac tgttttgagg ttgtagatgg aactgacgta gtcagatcaa
      601 aacactgttt tgaggttgtg gatagaactg acgtagtcag atcaaaacac tgttttgagg
      661 ttgtggatag aactgacgaa gtcagatcaa aacactgttt tgaggttgtg gatagaactg
      721 acgtagtcag ctcaaaacac tgttttgagg ttgtggatag aactgacgta gtcagctcaa
      781 aacactgttt tgaggttgtg gatagaactg acgtagtcag taaccatacg cacggcaagg
      841 cgacgttgac gtggtttgaa gagattttcg aagagtatta gaaagctcga tgaactattc
      901 cagattcatt ccactaaatt tcgaaatcga gtaggggata atctctagcc cctctcacac
      961 caccgtacgt gccgttcggc atacggcggt tcaactaact tttaacgcat gtcgttcaag
     1021 gtaataatcc aaacacgaaa ccagtccacg tttttcaagg actggttttg atatagcacg
     1081 tttaagtacc gacttctgag ctactaattg atagtggtcg ccccagccag ataccttatc
     1141 cgctatatag gccttattat cacgttcctg ttcgtagagc cacgatttcg ctatcccttc
     1201 ctctgacgat tcttatttcc agcgtcagac ttaaaacggt ctatccccag accgttttaa
     1261 tccgctacct cacgatagtc agacttggga atcgctattg ggttcaccgg tagtaggtgc
     1321 ccaccgagga cttacacctc agatatacga catgcccgtc gtacatcaaa aaaagttgag
     1381 acatagtgtc ttcaactttt ttacattact ttaactactg attaaagtga gtttacgtca
     1441 actttgatac caacaccttg agtagttgtg atagtcaagt ttgttacgta agttccttta
     1501 gctgtagctg gttttgcttt ttggattgtt tcgttgaaag ctttaaagtt ttcaaccaat
     1561 ttttcagctt caaatgatac tttaccgatg attgcttgaa cgttacctgc acggtcagca
     1621 cggtaagtga ttttaccacc tttagactct tcaactgctt tagcaacatc cattgttaca
     1681 gtaccagttt tagggtttgg catcaagtta cgtggtccaa ggacacgtcc aagacgtcca
     1741 acaagagcca tcatatcagg tgtagcgata actacgtcga agtccaacca accgtcgttg
     1801 attttagcaa caaggtcatc ttcaccaaca aagtctgcac cagcagcttt tgcttcttca
     1861 gcttttgcac cacgtgcgaa aacaagaaca cgtgaagttt taccagtacc gtttggcaat
     1921 accattgctc cacggatttg ttggtcagct tttttaacgt cgatgttcaa gttgtaagca
     1981 acttctacag ttgcatcaaa ttttgcaaag ttagtttctt ttgcaagtgc tacagcttct
     2041 tctacactgt atgcttttgt gctgtcgatt ttctcaagag cagcacgaag ttgtttgctt
     2101 tttttagcca ttttctattc tccttgtaag tggttcaatc gattttcatc tcccacgtca
     2161 cttctcgaaa tgatgaagtc ttgcgggttt tcagtctact atttttctct tcctacggcc
     2221 ttaagctccc ttgctgtacc caagtacaag cctcagtcac ttgcctagta tgaaaagcaa
     2281 ctaatagact gctgtgagat tttctactgt gttattgatt agtcaacaac agtgaatccc
     2341 atagaacgag cagtaccttc gatcatacgc attgcagact ctacgtttgc tgcgttcaaa
     2401 tctggcatct tagtttctgc aatttcttgt acttgtgcac gagtaactgt agcaacttta
     2461 gttttattag gtgtacctga tcctttttca acacctgcag cttttttcaa aagaacagca
     2521 gctggtggtg ttttcgtaac gaaagtaaat gatttatctt cgtaaactga gataacaact
     2581 ggaatgatca taccagcttg gtcagctgta cgagcgttga actcttttgt gaatcccatg
     2641 atgttgatac cagcttgacc aagagcaggt ccaaccggtg gagctggtgt agctttacca
     2701 gcagggattt gcaattttac aagtttttcg acttttttag ccatttttaa atcctccttt
     2761 gtggttttgg cggtaattag agatttttac ctcccacaag tatgtttttc acatacccat
     2821 ccattataca ctatttatct ggaattgcaa gagaaaagtt tattttttgt actcaatgaa
     2881 aatcaaagag caaactagga agctagccgc aggctgctca aagtacagct ttgaggttgc
     2941 agataaaact gacgaagtca gctcaaaaca ctgttttgag gttgtgaata gaaccgacgc
     3001 agtcagtagg taaggcgacg ctgacgtggt ttgaagagat tttcgaagag tattaatcga
     3061 cgtagtctcc gccttcaaag ccatagtatt cttccaaact gagaccactt gcagcaattt
     3121 ctttagcttc ttttcctaca taacgcaggt gccattcttc agccatatag cctgtttcct
     3181 tttccttgcc tttgagataa cggacaacaa agccataatc agctgcatga tccaagagcc
     3241 attgggctgc tttttcttct gtcaccaaat caccatcagt cccaatcaca tcaaaggcca
     3301 agcctgtctg gtgttcgcta tagccaggac gggcagagta acggtcagct gctgcctttc
     3361 catcttggtt gacataatct tgatagagct tggtctgagt ttcataactt ctaaaaccac
     3421 tgtaatgatc actaataggg aaacctgcct cttgcatcgc tttgatgagt ttgaccaact
     3481 ctgccttggc tgttggattt tcccctggat tatagtcttt agacaatgga tagtgtttgt
     3541 tggctacgat gatttcatcg tatttccctt gaatgctgta gtaatcacct ttattgacca
     3601 cttctgcttt cttctgggca gctccttgag acttactacc gactgttcca tcagctttgg
     3661 ctgtctgttc tgtcttagtt tctccatctt cattttttgt tttttcttgt gaacaagctg
     3721 ctagactcaa ggctagcaaa gctgtcaaga ctaggtatct tttcttcatt tctactcctt
     3781 tatttctaat agtttatcta cttctgacga taaacgtttg actaatcttt taatctcatc
     3841 cggttttgct tggcaggttt ctgctgtcat ttcttcaaag ggaacccacc agactggtcc
     3901 acgacccttg agaccatgac cattcatatc acctgtccgt cgtggaaaca gatgccaatg
     3961 aagatgagca tcgccatttc ctagcagttc gatattcatt ttctcagcag caaaggcctt
     4021 ggcaactgcc tcttggacta aactcatttc ttctagaaaa cggagtcttg tctccttttt
     4081 caaatggtgc aattcgctga catgctcctt ggctagaaag agactatagc cttcaaaata
     4141 ctggtggtct ccaaccacaa gatagcctgt ttccaactct ttgacaaagt aaggattttc
     4201 ttccttcttg atgaggtcaa ttctctgaca aataaggcac atattagtcc accaacgtgc
     4261 tcttaagata agcatcgacc attcgttctg ttgcgatcac cgagtcaata tgggtacgct
     4321 cataggaatg gctagactct ataccagcac cgagaagggc gtgtttgact tctgcccctg
     4381 cagacatagc cgctgaagcg tccgaaccat aaaatggata gatatccagc ttaaatggaa
     4441 tatcttgctc tttcgccaaa gccaccaaat gttgacggaa gtcatagtga taaggtccag
     4501 aagcatcctt gacacagata gacactgtat attcgtctgt ttgctggtca tctcccatgg
     4561 ctcccatatc cacagccaga tattctacta cctgagcagg aatgttagag tttgcaccgt
     4621 gtcccacttc ttcaaagact gaaaaagcaa aatgagttgt tacgggcaat tcaatcttct
     4681 cttccttata aatgcgaagg agattgagca aaatcgccgc actgaccttg tcatccaaat
     4741 ggcgagactt gataaaacct gtctctgtca cgacagttcg tgggtcaaaa ctgataaaat
     4801 caccgacctc aatgccaaga gcacgagttt ctttttcact agttactttg gcgtccaaac
     4861 gcacttccat attgtcttgc gtgcgttctg cagttcctgc atccttatag acatggcaag
     4921 aagtttggtg gatgaggatg gttcctgata ctttttcacc tgtgctagcc acatgaatgg
     4981 tacagttttc tccttcaatc atgttccaag gaaagccacc gatacggtcc attttgagac
     5041 ggccgtctgg tttgacagca cggacaatag caccaagcgt atctacatgg gcagtcacat
     5101 agcgatgttg ctcatcattt tgacctttaa tagttacatt gacaccgccc ttggatgtgc
     5161 gaaccggctg gtaaccaaaa ccttctagag tcttaactaa atagtccgca atctcacgag
     5221 taaagcctgt tggcgacgca atggctgtca gttctttgat atattctact gtttgattca
     5281 tttcttcacc tcttttgcta cctattataa cacatttatc atcggataaa aaaagaaaat
     5341 cactcctgta gacttgacta caaaagtgat tttatactca atgaaaatca aagagcaaac
     5401 taggaaacta gccgcaggct gtacttgagt acgacaaggc gacgttgacg cggtttgaat
     5461 ttgattttcg aagagtatta gtgattattc catttcaaaa acatcgcttt cttgcttgtt
     5521 tggtagaacc aatgagagca agattccaac aatggctggc accaaccatg ggagagatgc
     5581 cttggcaaaa ggaagaacgc taacaagatt tgcaagaaac tcaaccttaa acgagcttcc
     5641 tagtacgctt gcaatggcaa taactgtaac cacagcaatt gtcaactgca tacctggttt
     5701 tgaaagagca acaaatttgt tgacaatgac aatcataacg atagcaatcg tgattgggta
     5761 caagataacc agtactggaa ttgagtactt gataatcgca tcaagaccca aattggcaat
     5821 agcaaatcca atcaaggtaa aggctgtcgc ataaaccttg tagctgattt gtgggaagcg
     5881 ctcattaaag aactcagctg ttgacacaat caaaccaaca gtcgttgtga agcaggttac
     5941 ggtaaccata gctgcaagga agagttgagc tgttgagcca aagatttctt gagtggcttg
     6001 tgacaagatg taaacacctg gtgttccacc cttcatcgct tcagctggta ctgggaaatg
     6061 atttccaaga aaacctaaac cgatgtaaag agcgctgaag gcaagggcaa caacgatacc
     6121 aacaacccaa atagttgaaa tgtattcttt cttacttgaa aatccaagtt gtttcaaggt
     6181 ttgaactgcg attacgctaa aggccactga ggcaagggcg tccaaggtat tgtaaccttc
     6241 taggaaacct gtaccaaagg cagaagcttg ataagcagct gaagcagctt gaggacttgt
     6301 tccaccatat ttgatagctc ccagaacgac caagataaca atcaaaattg caaagactgg
     6361 cgttaaaata cgtccaatgc ggtctaagat ttttgatgga ttaagcgaaa tcaaataggc
     6421 tgccgcaaaa tacagaaccg taaatacaat caagccaagt cctttatttg catccgacaa
     6481 aagggggcta atccctactt cgtaagctgt tgtagcagta cgtggggtag caaagaatgg
     6541 accgattgac aagtaaagaa ctgagaggta aagagtcgca aaccaaggcg ctatcttcgt
     6601 tgaaatctcg tagatatacc ctttaggatt tagcgttcca ataataaggg tcaagacggc
     6661 gataccaacg cctgaaaaga caaaacctgc gatggcagga agaaaatgtt ctccagatag
     6721 agcacctaga gaaggcggaa aaatcaagtt ccccgcacca aaaaatattc caaacaggag
     6781 caaacctgtt agggtacctt ttttagccat aaaaatctcc ttcatattta taaaatactg
     6841 acctagtata ccacgattag gagtctgaaa aaagcatcac gaggcaaata ttaaatgttt
     6901 tcaattttct taaaaataag aattatctaa aaataaaact ctcctaccca gtccacacca
     6961 agtaggagaa atgcaaaatt ttaaagttct tcaaaggcta acatgccacc ctggacattg
     7021 ataacattat aaccttgttc taacaggaat tggcaagcac gcgccgatct cattccagat
     7081 ttgcaaataa tataatgcaa gcgatctttg tccaattaat cataactatc agtcaattga
     7141 ctaagcggta ggttgtgggc accttctaaa tgaagagctg caaactcatc cacttctctc
     7201 acatccacta gagaaagctg gtcgttttgg taaagctggt aaaatgcgtc aaaggctatt
     7261 tctttcattc tttttcttct aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc
     7321 cttaccgtgc gtatgttacc gacttcgtca gttttatctg caacctcaaa gctgtacttt
     7381 gagcagccta cgactagctt cctagtttgc tctttgattt tcattgacta taaaatggtt
     7441 ttaattcttt ttttcaaatc tggcactact tctgcctcaa accaaggatt tttggccatc
     7501 cagatttgat ttcgtggtga tgggtgaact agcggaaaat aggctggcag atagtcttta
     7561 tagtgtttca ccctctccgt taccttccca ctgattttct cctgtaaata gtaggcttgg
     7621 gcatattgcc caatcaagag ggttaactga atatcaggca attcctgtaa gagctgcgga
     7681 tgccattttt ctgcaaaacc tgtacgaggc ggaagatcac ccgacttgcc atgtcctgga
     7741 aagtagaaat ccataggcaa aacagcaaaa taacctgaat tgtaaaaggt atcttcatcc
     7801 acacctagcc agtcccgcaa gcggtcacca cttttatctt tccagtaaag gcctgcttct
     7861 tgagttttaa gtcccggagc ctgaccgatg atattgatgc gagcagtttt tggcgctgca
     7921 aagagaggct caatgccacg ctctgtatag ctggcattct gtgaatccgc catgatagcc
     7981 tgtttgattc tttcgatttg agacatctac tttccctcca aaaagaagtc caagcataag
     8041 atctcagact tctatcgttt tatttgatca attcgtaaat agcttcggca taaattgctg
     8101 ctgctcggaa aagatcatcc aaggcgataa attcattggc ttggtgcatg gtatcaatcg
     8161 agtctgggaa catagcaccg taggcaactc cgcgttctag caagcgacca aaggttccac
     8221 caccgatgac ttgttcatga cctttaaggc cagtttgttt ttcatagata ttcaacaagg
     8281 tttgcacaag tggatcttcc attggcacat agtgaggcgt gtgaccgtgt tcagacaggc
     8341 taacagaaac aactggcaag ttttcaagga ttgacttgat ttgttctgga cttgttcctt
     8401 ttggatagcg gatgttgagg gcaatggtat tatcagcact tgtttcatcg aagcggaaga
     8461 cgccggcatt catagaaaga gcacccatct tttcatccac atgagcaatc ttaagatttt
     8521 caccctcatg atcgttcaag agaattttac ctgcgatgtc aaggtagtct ttggctggac
     8581 cagcaaagcc aaactggctg aggaagaggg caaggtaagt tgcgccattg acacctgaag
     8641 caggcatagc accgtgggct gatttaccaa tgatcgtcac cttgtatttg ccagcttctt
     8701 cttggagttc tcctctaagt ttgtgttctg caacaaaggc atctagttta gcttgcaagt
     8761 cagccaagtc acctgaaacg actgctgttg ctgattctgg taccatattt tcacgtaaac
     8821 cacctgtaaa gctgtgaaga cgggcaacac ctgtattttc tcctgcaaag tggaggtatt
     8881 ccgtgatatt tcctttttca ccattgatga ttggaaattc agcatctggt gagaaaccga
     8941 aatctggttt ggcaagtcct acgtgctcaa agtagtagtc catgtctgcc cagcctgatt
     9001 cttcgtctgt tccaacgatg aagcgaactt tcttagaagt tggaagaccc aattctttga
     9061 tgattttcaa accatagtaa caagctgttg taggaccctt atcgtccgaa gccccgcgcg
     9121 cataaaggcg accatctttg atagttggtg tgtaagggtc tgtgtcccaa ccgctaccag
     9181 caggcaccac atccatatgg gcaaagattc cgagaacttc ttctccatca ccaaactcaa
     9241 aatgtcctgc atagttatca acattcttag ttgggtagcc atcgcggtct gcgatttcaa
     9301 ggaatttctc caaggctttt actggaccag gcccaaatgg atgctgggca tcagccttgc
     9361 tgtcatcacg ttctgaattg atttccaaaa ggctaaacaa gtcagccaag aggtcttctt
     9421 tgcgtttttc tacttctgct gtaaaatcaa ttgctgtcat tttttcttct ttctatcttt
     9481 tctcgatgat ttcatctact ggcaagcggt agcttggttc caatttttcg tctgtataac
     9541 ccactgtgat caagagttct gggcggaaac ggtcttcgat ttccaaaact tcattaactt
     9601 ttgatttgtc aaaaccaagg ataatattgg aaccaattcc ttggtctgta agagcgagaa
     9661 ccaagttcat agcaaccaaa cctgcattga gtgctaggta gtcgctgact tgttgttcat
     9721 tgtagcgtgc aaattcagct ggcaaatttt tcatgaagta ttgaagttgt tcttctgaga
     9781 aattgttagc accaccaact cgggcaatct tacgagcgcg tttggcaagg tctgtatctg
     9841 taaatagggc aatggttaca ggtgctgatg atacctgttc aaaattggaa ccataagcta
     9901 actttgccag ttcagcattt ttctcacata ccaccacaaa tttccaaggc tggctgttgt
     9961 gggcgcttgg cgccaaggtt gcaatttcga tagccgtacg cacatctttt ggatcaacag
    10021 gcttatcagt gaaatgcttg gtcgcatgac gttttttatt taattcaaga aatttcataa
    10081 tcgatttcct tttctaattc tactgcttcc attctaccat aatttgaaag agcttgcagg
    10141 gatttttcat gattaagttt ttctatcaca gacataaaag atatatattg gtttatcagg
    10201 aaagtttctg ataaactagc aagtacttta catttgaatg gagacagtat gaaaaaattt
    10261 agcctattac tagctatcct accatttttg gttgcctgtg ggaatcaagc tacacccaaa
    10321 gagtccagct ctcaaaagac aatcgtcctt gctacagctg gcgatgtgcc accatttgac
    10381 tacgaagaca agggcaatct gacaggcttc gatatcgaag tcctaaaagc agtggatgaa
    10441 aaactcagcg attacgagat tcaattccaa agaactgctt gggagagcat cttcccagga
    10501 cttgattctg gtcactatca agcagcagct aataatttga gttacacaaa agagcgcgct
    10561 gaaaaatacc tctactcgct tccaatttcc aacaatcccc tcgtccttgt cagcaacaag
    10621 aaaaatcctt tgacttatct tgaccagatc gctggtaaaa caacacaaga ggataccgga
    10681 acttctaacg ctcaattcat caataactgg aatcagaaac acactgataa tcccgctaca
    10741 attgattttt ctggtgagga tattggtaaa cgaatcctag accttgctaa cggagagttt
    10801 gatttcctag tttttgacaa ggtatccgtt caaaagatta tcaaggaccg tggtttagac
    10861 ctctcagtcg ttgatttacc ttctacagat agccccagca actatatcgt tttctcaagt
    10921 gaccaaaaag agtttaaaga gaaatttgat aaagcgctca aagaactcta tcaagacgga
    10981 acccttgaaa aactcagcaa tacctatcta ggtggttctt acctcccaga tcaatctcag
    11041 ttacaataac actcttcgaa aatctcttca aaccacgtca gctttatctg caacctcaaa
    11101 gctgtgcttt gagcaaccta cggctagctt cctagtttgc tctttgattt tcattgagta
    11161 taagatatat aaaagcgatt ggactaccaa tcgtttttag tttgcattgg ttattttagg
    11221 aaactcttac aaaaaatcaa aaaaattctt cacatcctct acatacctat gcttttctat
    11281 taagcttgct aatagtttca gattgtgcct ctgatagtta tcttcacgac gtagttcttc
    11341 atacatttcg ataaagggaa gccccttgga tttggccaat tctaactcag cctcgtaatc
    11401 ataagcaact ttacgaaatt ggatattgag taattcccca tcttcaacct ctatcacggc
    11461 atactgggca cggtgatttt ttaacgcctc ccaattaaaa tagggcatgc caatcgaccc
    11521 tggattgatg atttgttgcc ctttactgcc gtagcgaagc aactgcttgt gaacatgacc
    11581 ataaactgcc acgtccgttt ccgcatctag cagttggtca aatttctctg tatcattctc
    11641 aactagcaag tcaccaccat agtttttgtc aggtaaatta tgagagatag aaaagcgcaa
    11701 tccgtcaatt tctttctttt ccagcaaagg caagcttcgt agccagacaa tcgttgcagg
    11761 atccattcgc tccatcaaaa actgggtcat gcgcattgac tggatttctt tcggatattc
    11821 caaaccatat tcgccatcca agacctcaag gacacgatca tcccaattgc ctcgaacact
    11881 tgctgtgata agaaggtcct ttagcagggc gactaagtca tttgcgcctg gaccaggaag
    11941 aaaaatatct ccctgaagcc aatattcact ggccccttga tttttagcat ctgcaatcac
    12001 tgcttctagc gccgtcgcat tgccatgaac atctgataaa attgcgattt tatggttcat
    12061 tgtggtttcc ttccgtttgg taatctactc tttcttctgc tacttgaggc aaggcttctc
    12121 cctgcgggtt caactttctt tgcacagcct ctgcaacgac tctaggtacc ccaacttcga
    12181 caatctcatc cacactggct tccttgattt tggtcaaaga cttgaaatgc ttcataagat
    12241 tctgcttgcg tttaggtccc agaccgtcaa tcccatccaa ttgagatgag aaagaatttt
    12301 tggagcgcag ttggcggtgg aaagtgatag caaagcggtg cacctcatct tggatgcgtt
    12361 ggaggaggaa aaattcctga gaattgcgag acaagtccac cacctcaagc ggatctccaa
    12421 agagcaattc atgggtttgg tgcttatcat tcttttgcag cccagcaatt ggaatatcca
    12481 agcccagttc ctcttggatg acttgcttag caatattgac ttgaccttgc cccccatcaa
    12541 tcacaatcaa atctggagga gtcaaagcct cacgctgtac tcgaccatag cgtctgcgaa
    12601 tgacttctct catactagca tagtcgtccg gcccaacaac ggtttttatc ttgtacttac
    12661 ggtaatcttt tttacttggc ctgccgttaa caaagaccac catggctgaa acaggactgg
    12721 tccccatgat gttggagtta tcgaaggact cgatgcgaac tggagtcgga atttggagca
    12781 aacgtcctag attttcaata gctccttggg tcttttcgac tgatttttct agcagattga
    12841 acttctgctc tagactaaca cgagcatttt ttatggctag attgaccagc tgttttttct
    12901 ctccacgttg gggtttgaga atcttggtat ccaccaaagc cttgacagct tcttcgtcaa
    12961 tatcctgcgg aatcagtacc tcattgggaa ctaggtgaga tttttcttga tagaattgtc
    13021 ccacataggt caagaaatcc tcatctggat cattgaagta ggggaagaga ttgacatcgc
    13081 gctcgatgag cttgccctga cgaacaaaga aaacctgcac acacatccag cccttatcca
    13141 catagtagcc aaagacatcg cgattttgga gatccttagc catgacccgt tgcttggttc
    13201 gaagtgttcc aatggcctga atcaggtcac ggtattccgc tgcccgttca aactccatac
    13261 tttgtgctgt tactgccatt ttactcttga gatcatcgat gattttgtca tcctgacctt
    13321 tcagaaaatc agacacctcc tgggccatgg acttgaaata ggcctcgtca ttcttacaga
    13381 tggtgtgggc catacattgg ccgatatggt agtaaaaaca gaccttagag ggcgggttgg
    13441 tacattttcg aaaagggaaa atcctatcta gaagtcgctt gatttcattg gctgccccca
    13501 catctggata agggccaaaa taaagaccac cgtccttttt gacctgacga gtaatgataa
    13561 gccgtggata gtgctcattg gtgattttga taaaaggata agacttgtca tccttgagca
    13621 tgatattgta cttgggctta ttttccttga tcaggttgat ttccagcaga agcgcctcaa
    13681 tattagactc cgtaacaata aattcaaaat ccacaatttc agacaccaag gcctctgttt
    13741 tggtatcatg acttccacgg aaataagacc tcacgcgatt acgcagattt ttagcctttc
    13801 ctacatagat aatggtgccg tttttatcct tgtaaatgta gcaaccaggg ctggtcggca
    13861 agagctctag ttttggttta atcaagttgt tcatagttcc attatagcaa aaaagtaggg
    13921 aaagatagta ccctactcat tagtctcata tatttttcgg aggtttgcaa catcctcttc
    13981 ctggatacca taaaaggaac ctgctggttg catgacagac ttctcatctg tatggtccaa
    14041 gccaatcgca tgacctaact catgttctgc cgtatggaca aggcgttcat aggaatagcc
    14101 atagtatgga ttggacaaat aataatgatt caaccgcacc gttacggaca agaattgccc
    14161 tgttaagaga ttagtttgac tttccgcctc tcctgccaca ggagtgcctc cgtcgttcat
    14221 ctccgtagcc gtaatatccg ccttactaga ctcagtcacg agttcaaagt taaaagcacc
    14281 agtttgattc cagttctgga ttgcttctaa ataagcctct tgaaagcgtg aatccatctg
    14341 cggatccagg taaatacgag cagaggcctg tggccacctt ccttcagaat gctggtgact
    14401 atctgtctgg cttaacttag gcagttgccc tgttttctcc cattggtcaa tactttgttg
    14461 accaattttt actgaccgtt ctgcttgctt tagcgctcct tgaaaatccc cgttcagata
    14521 ccagagaagt ccgaaagcaa gcacacataa gagcacaact atccaaacca gacgccaaaa
    14581 caaacgccac acaaaagaaa agaaagcccc tatcaaacga aaaagccagc gcatgtctct
    14641 ccacctttct agcaaataaa aattatttta ctaaaagaag ctaaaagaaa tctaaatact
    14701 gactttttca tcttaacgtc ctactatttt tttgagtaac tggatagctt tgtattttaa
    14761 aggagatgga tggtaacgga aagtacctgc tttgcgtaca atatagccat taaaattctg
    14821 tttaaaacgc aaaacaccat cacttccatc aaaaatccct tgaatgccta ggaagttgta
    14881 tttaggtatt ccacgtttta tgctttccaa cataacatat ttttgaagca gtgcaggggc
    14941 atagaactta ttaaactcag tgtaggaacc actaaagaga taagtcgttt cctgaggcat
    15001 ataaacaaat aaactcccag ctaaaacaat atcttcttct ccatattttt caatcaagtc
    15061 tcgcgcttct gcttttcgaa cttcaaacgt ttcaaattga ctagaatatt ctctcagttg
    15121 attttgtttt ttctcagaat gaggattttt actcaaatca agtcgcaact tgtccaagtt
    15181 ttcttctagt ttattttgtt caccttgcaa tttgctcata tagtccgaaa aatttaagct
    15241 tgctatgaga aactccgctt gttctccaaa agtatcataa aaatgctcat aatattctaa
    15301 acttttatca ctatattctc tacgttcaga ggtttctttt gttatattct taaaaatcga
    15361 tagttcttca cgttttaact ttttcaatcg aatgccaaag gtttcagcct ttttcaccaa
    15421 gggtttaccc tttttgctaa aacttttaag caaactcttt tcagttaatt cagttaaatc
    15481 tttatagtat aaccaatctg gttctccacc tgggtaacct gttgttaagc catcaaattg
    15541 ataacctaaa tcagtcaaac cttgaataat actttttttc tcagcatcta ttggattacc
    15601 ttggctatca aaagtttgat aagtttcata aggttttaca agcaactcta atacaccatt
    15661 ttgcttggca tattctttta actctgcata aaaaactgga agagcatctt gttgggtata
    15721 aatcggcccc gaattgagtt ccatatgcag accacccagc atgggcaagc tataaaccag
    15781 agctgcaact tgaatttctc cttcttgttt caaagcaaga taaacaattc gagcccctct
    15841 tttttctagc aaatccccca tctggacaga ttgcataaag gaacgagaag aaacctgatc
    15901 agaataagtc tgaaactctt ctttcgtgag tgttgttagt gccatatact tactttctat
    15961 gtttttttct taatgtttta cggaaatcaa gagcaagtct taacagagga tagagaggat
    16021 gagtgggcat tgtaaattca cccaagtatt cttcaatcgt tggattaaat ttttccttaa
    16081 aatgataaag tccaccattg agagagtttt caacaccacc taaattttgc cagaccatac
    16141 ctcgctcaaa ggcatagcga gccgtttcat accatgttaa aattggtgca ttgtaacgtt
    16201 taaaatcatc atccatacca gcatatatat tgacagaggt agtaccaaat tccaaactca
    16261 aagtagccgc taaaggaact ctcgcttgac ctacatctat atattcctgc aagaaggtca
    16321 attcctctaa caaacgttct ttttccttct tctgcgcttc tacttttgaa gttcgagtcg
    16381 actcagtaaa ggtctcttcc aaggctctat ttttcgctaa ctgttcttct aattcttgcg
    16441 aacgtttaga aacatccaag gtggccaagg tgatataggc cttgtcctta aaattatcta
    16501 acaatttttt ataataggct tcattcctca aatgaatctc ttttcgcttc tcagtttttt
    16561 tcatcaactc cgaaaatgaa tctaatagtt ccagtccacc atattgaatc tcaagccctt
    16621 tgtttcgtgc tgttcgaata gcctgttttg ttgacttgga aagtttatct tcttcaaaat
    16681 tttccttgta tattttcgcc tgaatacgag gttgaatggt gtctcccatt tcctccgttt
    16741 ttcctgacca ccttactccc atttgttgca aactatcaat aatagccaga ttttcaggaa
    16801 attctgtctt ttcctgattg attaaacttt gagataggca aatacttggg tcaaaagtca
    16861 caaaaaccgc tctcttactg cgagcatagg acttaataga ctgaatggca aaattcaaga
    16921 gttctttatc cccataatcc aatataggtc ctcttgggat gtaaaacatt ttatagccta
    16981 gcggaagagt tctaatcaaa atactagctg tcgccagtaa tttttcttcc ctgtaaacac
    17041 caaacttctc atgttgccaa ttagacttaa cttcctccca agcactactt tgtaatacat
    17101 tggctaattc atgttctttg acaaactgat catattctaa tgtgggaatg ccaatttgat
    17161 aacggtacat ttcctactct ctttcctcca gtattttatt actattctac tactttcgtt
    17221 tgaaaagtct agtgaaaaca tattttattt tagttctact ccccctatgc tgaagttcaa
    17281 actaagtcag tctctcttct aatttgaaat caattggtag ggttgttaaa aaaacttcca
    17341 attgtttttc ccagtagtac cactcgtgag ttccagcact atggctatag gtcacatcaa
    17401 aacctagttt tttgagattt ttcactgcga gattattggc ttcgtacaag aaatcctgtt
    17461 cgccacacca cgcccaaagt ttggtctttt tatccgattt tttagccaga ctttcaagag
    17521 aatagggact agttgtccag tctctaatct ctccaaaaac acctctccag taggctggac
    17581 ttcccagatt ttgactttca ggagaaaagt tttgaaagct gagggcacct gaaaaactag
    17641 ctgcatgaga aaaacgattt gtcgtaagag ccagtttgaa gcagccgtag cctcccatag
    17701 aaagaccagc gataaaggtc ttttcacgct tgctagtcat attagggaag aagcgtttca
    17761 gaacctgtgg caattcctct gctagagccg tgtagtagtc aaaaccatac tgggtatcgg
    17821 tgtaccaacc attgctggta ttgggcataa caacgatgag attagttcct cgaagcaagc
    17881 gttctacatt ggtccgctta agccaactat tatgatttcc agacatcccg tgcaaaaggt
    17941 acaagacggg aatatcttca cactctggtt cttccactcg attggcatca gggtagagga
    18001 cattcacccc ccactccata tccaatactt gtgagtaata ctcgattttc attactgcca
    18061 tgattttctc cttctatttt tctataagaa aaagccgaaa ctcgactttc tctctgttta
    18121 tttcaacgat tattttgctt ccatgactac tggtaaaata gctggacgac gcttggtttg
    18181 gtcaaagaga tacttggtca aattatctcg aaccttccct ttaagatctg cccagtcaaa
    18241 gtcatctcct tgaagataat cttctaccgt ttggttaatc aattctgaac tttcacggag
    18301 aatatcgcga ctcttcttga gataaacaaa tccacgcgtg tgaacacgag ccctagccac
    18361 aattttcttc tcacgacggt tgactgtaat agccacgatg aaaattccat cctctgacaa
    18421 gaccttacgg tcacgaagaa caacatttcc aacatcacca atggcattcc catcaatcaa
    18481 gatatctcct gctgaaaccg atccagctgg aacaaagtct ccattctcgt aagccatggt
    18541 cgtccccttt ttaggaatga aaatgcgttc tggcaacatc ccaactgcca tggcagcctt
    18601 agcgtgagca tccaactcac gatactcccc ttggacaggg aagaggtact taggttgcaa
    18661 aagattgatc atcagctgca aatcacgcac atttccgtgc cctgatacat gtaaactttg
    18721 ggtaatcaat ttgacaaccc cacctgcctg ataaatcata ttttccacac gcgcaacaaa
    18781 ggcttcttta gcaatagacg gagccgtagc aatatagacc aggtccccat ccttgatttc
    18841 tacataacga tggcgaccaa tcgacatctt acgaagtcca ttgataggtt cacccatacg
    18901 acctgtctca agaataatca actcatggtc ttcaaagcga gacatatctt taggcttaat
    18961 caagagaatt tcgttggcta aagacaactt cttaagacga atcgctgtgc ggacgatatt
    19021 ttcaatatca aatcctgtca agacgatacg tcgacctgtt ttatccgcag cgtcaaaaat
    19081 ctgctgaata cgagaaagat tactggaaac agctgcaacg atgatacgac cttcccagtc
    19141 agcaatagtt tgggtaattt catccctaac ttcactttca ctagccacct gaatattgct
    19201 gtctgcattg gccgaatcac tgaggagagc caggacgccg tcacgaccaa tctctgccaa
    19261 acgagcaaaa tcagttgcat aagattcact agccgtttgg tcaaatttga agtcacctgt
    19321 ataaacgatg cttccttccg atgtcttcaa gacaattccc agactctctg gaacggagta
    19381 agtcgtaggg aagaaggaaa ccactgtccc accaaaatca atctccgtat tctcatcaat
    19441 gacatggaaa tcattaaatt tcttaacggc atcatttcct ttgacaaaga gctttgccaa
    19501 ctcaatggtc aactcagacc caaatacagg aactttagcc tctgccaaga gataaggtag
    19561 agcaccaatg gcatccgcat gcccgtgggt caagaaaacc ccagcaatac ggtcgctatt
    19621 ttcaaaaagg taatccatgt ttggaatcac cacatcgacc cctaattgtt cattttcagg
    19681 atattttaac cctacattca aaacaaaaat ggactctcca atttcagcaa tgtacatatt
    19741 ttttccattc tcacgcacac caccaagtgt tgttaaactg atattactca tttttcctcc
    19801 aaaatcttag tttatcttga ttatagggca attctaccct cttattctaa aagttctgat
    19861 actttcagcc gaatcttttc ctaccattat accatttttt tgatgatttt caaaatgaag
    19921 gtctgttttc aaaaaagagt tgatttccca actctttcct aacttctact ctttttccat
    19981 caaaaaatca taaatttctt gcaccgtacc cagcgccatc atttcttggt ctttaacggt
    20041 ttgtttgaga ttttggtaaa tttgactttc tcctatctcc cgctttggcg cctctttatt
    20101 gactgtcatc acaccatctt ttatcgtcac aaagcctagt tcttcaaata cttgaatcat
    20161 cttgaccagc aagatttgtt gaatattaag atatgcagcc aaatctttca gcttgtagcg
    20221 aatatcaaac tctgggaact ggtaaatagt cttgtacaat ttagcaaact gatctctagt
    20281 cccataacct gtcagataat aggtcttgtc aatatcattt ttgaaataga cagcagagaa
    20341 atgctgttcc tgaaaaatgg tcttcagctg agtaatatcc tctggaatgt tttttacgac
    20401 aacagcttca ctagccgcaa gatttggcag ttctccagga aaatccaaga ctggaacacc
    20461 ttctggcaag actgcatttt ttccacgaat gttaaaaagt tgaacacctt ccactcgcgc
    20521 atccaccatc atcaactgga gggcagtttg gccattccat tggttgacag acaatttaac
    20581 cgctaactct agattcttgg tttgagaaaa ctctgtcgcc catctgcctt gaccaaaggc
    20641 taccacttca aaactcgcct cacccttgga aattttcagc tttagatggg cattacctgc
    20701 ccccatagta cgagcacttt cgacctgaaa attcttgata taaaaaatag gtttctgatt
    20761 atccattcca aaaggagcta aacgttcaaa acttttgacc gtttccaagc taagtgcctc
    20821 caaatccaac tcttcatcta ggtttaactt attcttgcca ccagcatctg cacctttttc
    20881 acgaacataa tcttccaaaa cctgagataa atctgagagt tgctcaactt ccagcgtcat
    20941 acccgctgca cctgcatgac ctccaaaggc gatgaagagg tctcgatggg gatccagagc
    21001 ttcaaaaata tcgaccgctt ccacactacg agcactgccc ttggcacgac cgtcttctat
    21061 attaagaaca atgactgtct gtcccaattc ttccaataaa cgaccagcca cgattcctag
    21121 aaccccagga ttccagcctt ccttggccaa gacctgaacc ttcttctcag gatccaccat
    21181 ggtcttggct tcttcataga tagactgaac gatttccttg cgctcttcgt ttttctggtg
    21241 aatcataagg gcaatctcat gcgcttcctc atcatcaaat ccagtcaaca aatcaatggc
    21301 aggattggga tcatccaagc gacccaaggc attcaaacga ggagcaatct ggaaaccaac
    21361 cgtttcttct gttacttcgt tggcagcaat cccagccatg tccagcattt cttgcagacc
    21421 aatgcgctgg gtatgaccca acatttccag accatattga actaagatac gattttcatc
    21481 cgtcagactc accatatctg caatagttcc aatagcgacc aaatcaagca attccacttg
    21541 cacttcttct aacagggcac aagccaactt gaaagcaact ccacaaccag ccaaatattt
    21601 aaaaggataa tccgcatctg gatgttcagg atggacaata gcataagcat ctggcagggt
    21661 ttcaggcatg gaatgatggt ctgtcacaat gacatctact cccatagact gagccaatgc
    21721 aatagcctca tgaccagcaa ccccattgtc caccgtcaca atcaaggaaa ttccttcttg
    21781 ctcgataaag tatttataaa cactagcatt agggccatag ccatcggtaa aacgatttgg
    21841 caggtaaact cggcactcag caccaagttg ttccaaactt tccttcacaa tagaagccga
    21901 agtcatgcca tccgcatcat agtctccata aacgagaata ttttcccctt cttcaatagc
    21961 ctgacgaatc cgctccactg ccttgtccat atcatggagc agataagcat catgtaagtc
    22021 ctccaaggaa ggttctaaaa acttcttcag actttcttgg tcctgaatcc ctctctcaaa
    22081 caataaccga gccacctcag gacccagtcc agccttcttg gctatctttg taaaatccgc
    22141 atcttctacc tgcagggcaa actgccattc ataagtaggt gttatcaaaa agacatccac
    22201 tctttctaag aattctattg cttcattata acatgattta gacgttttct ctatccagat
    22261 tgctttttta taacatttta tagtatattg aaactagaat agtacacctc tacttctaaa
    22321 atattgttag aaatcgattt gactgtcccg atcgattttt cctgttctta tttcactata
    22381 gtgaattttt tcagatatga tttgacaaga acattctttt attgcagaat cgtgttataa
    22441 aatctaacat aaaggagttc tctcatggtt ttagtagaat ttaaaaacgt cgaaaaatat
    22501 tacggagact accacgcact ccgcaacatc aatctccgtt ttgaaaaagg acaagttgtt
    22561 gtcctgcttg gaccttctgg ctctgggaag tccactctta tccgtacgat caatggttta
    22621 gaggctgttg acaaaggaag tctcctagtc aatgggcacc aagttgctgg tgccagccag
    22681 aaagatttgg tacctcttcg caaggaagtc ggcatggttt ttcaacattt taacctttat
    22741 ccacacaaaa cagtgttaga aaacgtgaca cttgcgccca ttaaagttct aggaattgat
    22801 aaaaaagaag ctgaaaaaac cgccaaaaaa tatctggaat ttgtaaatat gtgggacaag
    22861 aaagattcct atcccgccat gctatctggt ggacaaaaac agcggatcgc catcgctcgt
    22921 ggtcttgcta tgcatccgga actcctcctc tttgatgaac caacatctgc tcttgatcct
    22981 gagactatcg gagatgttct agcagttatg cagaaactgg cgcgtgatag gatgaacatg
    23041 atcatcgtta cccacgaaat gggctttgct cgagaggttg cggaccgcat tatctttatg
    23101 gccgacggag aagttttagt agatacgaca gatgtcgata acttttttga caatccaagc
    23161 gaacctcgtg cccaacaatt cctcagcaaa attatcaacc acgaaagtga caaagtcaaa
    23221 taaggaggcg cctatgaaaa aaaattcttt ttatcagcat tattgattag ccttttcggc
    23281 cttgctgctg ccaaaccagt ccaggctgat acaagtatcg cagacattca aaaaagaggc
    23341 gaactggttg tcggtgtcaa acaagacgtt cccaattttg gttacaaaga tcccaagacc
    23401 ggtacttatt ctggtatcga aaccgacttg gccaagatgg tagctgatga actcaaggtc
    23461 aagattcgct atgtgccggt tacagcacaa acccgcggcc cccttctaga caatgaacag
    23521 gtcgatatgg atatcgcgac ctttaccatc acggacgaac gcaaaaaact ctacaacttt
    23581 accagtccct actacacaga cgcttctgga tttttggtca ataaatctgc caaaatcaaa
    23641 aagattgagg acctaaacgg caaaaccatc ggagtcgccc aaggttctat cacccaacgc
    23701 ctaattactg aactgggtaa aaagaaaggt ctgaagttta aattcgtcga acttggttcc
    23761 tacccagaat tgattacttc cctgcacgct catcgtatcg atgccttttc cgttgaccgc
    23821 tctattctat ctggctacac taataaacgg acagcactac tagatgatag tttcaagcca
    23881 tctgactacg gtattgttac caagaaatca aatacagagc tcaacgacta tcttgataac
    23941 ttggttacta aatggagcaa ggatggtagt ttgcagaaac tttatgaccg ttacaagctc
    24001 aaaccatcta gtcataccgc agattaagga ggatacccta tgacagattt atcatcttgg
    24061 acagcctatt ttcaggattt tggacaattt ttcaatggtt tcctctttac tcttgcccta
    24121 gcgattggat cctttatcct cgccatggtc ttaggaatct tctttggtgc catgtcaacc
    24181 agtaaacgtc caattttacg cattttagct cgcatctttg tggaatttta ccaaaatact
    24241 cctctcttgg tgcagtttgt tattgttttt tatggcctac ctcttatcag tgaccatatc
    24301 atcatgattc caatttattg gacagccgtt ctctgcgtgg gactctatca cggcgcttat
    24361 atcgctgagg ttattcgttc agggattcag tctattccta gtggtcagat ggaggccgcc
    24421 ttgtcgcaag gttttaccta tatcagtgcc atgcgcttga ttaccttgcc tcaggccttc
    24481 cgcatcattc tccctccatt gaccaaccaa attgttaacc tcatcaagaa cacctctact
    24541 gtagctatca tctctggagt agacttgatg tttgtgacta agtcttggtc ggctctcaac
    24601 ggaaactata tcccagcctt tttaggtgct actcttctct actttgccct atgcttccct
    24661 gttgcccagt ttggtcgcaa gatggagcaa gccaacaaaa aagcctattc actttaggag
    24721 gttactatgg aatctatttt agaagtttta accccagata acctagtctt tatctttaaa
    24781 ggatttggct tgaccctcta tatttctctg attgccatca tcctctctac tatcatcggt
    24841 acggtgctag ctgtcatgag aaatggcaaa aatcctgtct tgcgaatcat ctccagcatt
    24901 tatatcgaat ttgtgcgcaa cgttcccaac cttctctgga tttttactat ctttttggtg
    24961 ttcaaaatga aatccacacc agcaggtatt acagccttta ctctctttac atcagcagcc
    25021 ttggctgaga ttattcgagg cggtctcaat gccgtagaca agggacaata cgaagcagga
    25081 atgtcacaag gcttcacctc agcccaaatc ctctactaca tcattctccc acaagccatc
    25141 cgcaaaatgc taccagccat catttctcag tttgttaccg tgattaagga taccagcctc
    25201 ctctactctg ttatcgccct acaagaactc tttggagcca gccaaattct catgggccgt
    25261 tatttcgaac cagagcaggt cttcagtctt tgcatcctga ttgccctcat ctacttcagc
    25321 tttaacctag caatttctag cctgtctcat atgctagcca aacgttggca acaagctgca
    25381 gaataatact cttcgaaaat ctcttcaaac cacgttagct ctatctgcaa cctcaaaaca
    25441 gtgttttgag caacttgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa
    25501 acagccactc tccagttcaa ttggagagtt ttttgatgag agtagatatt tatagacctc
    25561 aatttgacac catacatttt ctatgataaa atgtaacaaa tttatttaca acaaatcgaa
    25621 atctaaatga aatggaattt tctatgaaat cagtcaaagg actactcttt atcatagcta
    25681 gttttatctt gactcttttg acttggatga acacttctcc ccaattcatg attccaggac
    25741 tagctttaac aagcctatct ctgactttta tcctagccac tcgtctccca ctactagaaa
    25801 gctggtttca cagtttggag aaggtctaca ccgtccacaa attcacagcc tttctctcaa
    25861 tcatcctact aatctttcat aactttagta tgggcggttt gtggggctct cgcttagctg
    25921 ctcaatttgg caatcttgcc atctatatct ttgccagcat catccttgtc gcctatttag
    25981 gcaaatacat ccaatacgaa gcttggcgat ggattcaccg cctggtttac ctagcctata
    26041 ttttaggact ctttcacatc tacatgataa tgggcaatcg tctccttaca tttaatcttc
    26101 taagttttct tgttggtagc tatgcccttt taggcttact agctggtttt tatatcattt
    26161 ttctatatca aaagatttcc ttcccctatc tagggaaaat tacccatctc aaacgcttaa
    26221 atcacgatac tagagaaatt caaatccatc ttagcagacc tttcaactat caatcaggac
    26281 aatttgcctt tctaaagatt ttccaagaag gctttgaaag tgctccgcat cccttttcta
    26341 tctcaggagg tcatggtcaa actctttact ttactgttaa aaattcaggc gaccatacca
    26401 agaatatcta tgataatctt caagccggca gcaaagtaac cctagacaga gcttacggac
    26461 acatgatcat agaagaagga cgagaaaatc aggtttggat tgctggaggt attgggatca
    26521 cccccttcat ctcttacatc cgtgaacatc ctattttaga taaacaggtt cacttctact
    26581 atagcttccg tggagatgaa aatgcagtct acctagattt actccgtaac tatgctcaga
    26641 aaaatcctaa ttttgaactc catctaatcg acagtacaaa agacggctat cttaattttg
    26701 aacaaaaaga agtgcccgaa catgcaaccg tctatatgtg tggtcctatt tctatgatga
    26761 aggcacttgc caaacagatt aagaaacaaa atccaaaaac agagcttatt tacgaaggat
    26821 tcaagttcaa ataagtaatc atactctcct gggatttccc aggagagttt tttatagcca
    26881 aatgaaataa gacaggaacg atggaatttt ggcattcaaa tcaattgata agaatgtttt
    26941 atagtggagt gaaataggat acgaacaaat tgattaggaa aatcaaatga atttatagaa
    27001 atcttttagc agttatgttg tcctattcta gtttcaaaac gctatagaag cagcattgtg
    27061 ctagtctaga ttcagtttac tatactaaaa cgagtagctt gaaatcaaaa aacccaccct
    27121 cacaggcagg ttttatctgt attattcagc tagattatgc tttaccttct gaaccgaata
    27181 cgtcgatacg ttcttcaacc gatgcttgga tagcttttac accgtcagcc aagaatttac
    27241 gtgggtcgaa gagttttttc ttgtcgtatt ctgcttcgtt tgcttcgtag tcacgagcaa
    27301 atttacgagt tgcgttagcg aatgcgattt ggcattctgt gttaacgtta actttggcaa
    27361 caccaagttt gatagctgct tggatttgct catcaggaat acctgatcca ccgtgcaata
    27421 cgattgggaa tcctggaaga gcttctgtca atttttgcaa gtggtcaagg tctagacctt
    27481 cccagtttac tgggtaaggg ccgtggatgt taccgatacc agctgccaag aagtcgatac
    27541 cagtttcaac cattgcttta gcgtcttcga ttggagccaa ttcaccttta ccgatgattc
    27601 cgtcttcttc accaccgata gtaccaactt cagcttctac tgagatacct ttagcgtgtg
    27661 ctttttcaac aacttcttta gccaatttaa ggttttcttc aactggaagg tgtgaaccgt
    27721 caaacatgat tgaagtataa ccaacttcga tacactcaag tgcatcttcg tagtgaccgt
    27781 ggtcaaggtg gatagctact ggtacagtga tacccattga ttcaacaagg ttagcgatca
    27841 agttgcgagc aactttgtaa ccacccatgt atttagcagc acccattgaa gtttggatca
    27901 aaactggagc ttttttagct tctgctgcgc gcaagatagc ttgagtccac tcaaggttgt
    27961 ttgtgttaaa tccaccaact gcataaccgt tgtcacgggc tgcttggaca aatttttctg
    28021 ctgaaacgat tgccatttta tcaggcctcc tgtatatttt tatgggtcat cccatttaca
    28081 ttgttcattt tatcactttt tgccaaaaaa atctagtttt tcccgcagtt tcgattgatt
    28141 ttcttctaac tccatctatg taaacccttt ctccctagtc ttggacgact tttggaaaat
    28201 ctataaagaa ggttaaacga ttctcctcca tctcgaaacg ataagctaat ttttcatgtt
    28261 ctaatagact cttaaccaca aagagcccca taccagaccc cttgaccttg cgactagcat
    28321 tgtcagaaaa agactgggct agtttttctt gttcctctga gctacagcta ttttcgataa
    28381 aaagttctcc ttctctttct ccaattcgaa ctaagccacc tggaacagag tgcttaatgg
    28441 cattgctgat gagattagaa agaatcaact tcataactga tgggtttaga taagcctgct
    28501 gatgggtcaa actattgtct atctggagct ctctttcctt tgctagcaag gcataatctt
    28561 tgaccagatt ttgcgtcatc tggaggaggt caattgtttc cctatcatct cgcaattcct
    28621 gcacagaaga gagggaaagt atctgcagaa catggtgatt gagttcatcc acaatcccca
    28681 aggcaactcc cagatactgg tctctatcct tataacgacc gatattctct ctcatatttt
    28741 cgattaggat tttcaaacta gccagcggtg ttttcaattc atgagaagct cctcgtagga
    28801 attcgacctt catcttctcc agctggagaa tggcttcatt cttttcatgc aagtccgcaa
    28861 taacagtcaa gagatgctgg tagaggctat tgatttgttc cttgagatta cctatctcat
    28921 ccttagaatc cacgcgcaat cgcacttggg aatccaggtc catcatccga cgggtcaccc
    28981 gcttgatttc caaaatcggt gcaacaatag tccgagcgta gatgtaggcc accaagaggg
    29041 aaatcagaaa ggaggccagc aaggtatagg gaagaaactg gagactgatt tgctccgctt
    29101 ccttttgtaa atccatggaa gctagaaact ggagaatcat agtaccaccg tcttgcgttt
    29161 tcacctcgcg ctcctcaata aagagagagg ttgtctggcg gtctgtgtcc agaggaagac
    29221 tgtccttgac ttctaacttg tcctcggtca tctcaccttt gacggtcccc ttgatatcac
    29281 tagtctggga atacaagtct aacacttgct cgatactctg cctatctttc ccttctaggg
    29341 actgggcaat ggctgttgcc ttttgaccaa tggtttcctg acgatgactc agataagtcg
    29401 aaggaaaaag aaaataaata gctaaatgaa ggcagataac cagaacacta aatatcgaga
    29461 aggtatagat aaatatcttt gcaaataaac ctgttcgttt cattttcgct ccaatttata
    29521 accaacattg cgcacagtga ggatacaatc caagtctagc tttttccgca attccttgat
    29581 ataaacatca ataacacggt caaagggaac ctcatctgtc gctttccaga cggcatcgat
    29641 aatctgagat cgagtcaagg cccggccttc atttttcact agatagtcca gaatttccaa
    29701 ctctttggca ttgataggca cttcttgacc tgcgaggctt gcactgtagc tttcaaagtc
    29761 caccttggta tccttgtaag aaaagattcg tcctgtatcg tagtagcgct tgaaaatcgc
    29821 gtccaccctc acttttaaaa gggagaggga gaaaggtttt tccagatagc catctgccaa
    29881 agaggcaaag gcactcatct tgtattcctc atcttgaaaa gctgtcaaca tcaagacagg
    29941 aacctgactg gttttacgaa tctcagctag gacttctaag ccgttgagct tgggcatctg
    30001 gatatccagt aaaaccaggg ccacctcata gctagaaaat tgctccagag cttcctgacc
    30061 gtccgctgcc tcaatagttt catagccaca atccgtcaaa taatcactga ccccctcacg
    30121 gatcatctct tcatcttcta caattaaaat tttcatactt taactgctct ctatttttta
    30181 tttttcttag aataaatacc taccctattt tctattatag tctcttgctg gccttttgtc
    30241 tgcaagcaac tgaccactag ataaaacgtt gtgaaattcc tttctcataa attccataac
    30301 tttagtatat tatatttaag cactaaagta caaagaaagc aactgaaagc aatgattttc
    30361 accactgctt tcggatttat tttgaattgt taaatagcca ttcctatcca ctattcttga
    30421 atagaaacac aagatgcaat ctttattcta gactcatttt ttcaaattta ttcaccatcc
    30481 agcaagagct cttttggttg ttttctaagg agattgcttg aagcaagcgc cataacgaga
    30541 accactagaa ccaaggcaag gacaaaaatg atgataaagt ctgatgtctg aatggaaatg
    30601 tctaggctcg acaaggtctt gctaaagcca tctacttctg caccaccacc aaggttagag
    30661 gcttgagccg ccttactagc ctgtttggca acacctgaag tcacattggc aaggacagtg
    30721 tttccaattg cacgggcagt gtaattagct aggaagtaag cagaaactag agcagggata
    30781 gcaatcaaga tagattcggt gatgaattga cccaagatac ttgcctgctt gaggccgata
    30841 gagaggagaa ttcccacttc cttgcgacgg gcgttgatcc aaaggctgag caagagggca
    30901 aggaggagaa ctgagaagct caagctaccc cagaagagga ggttggccat cttgtacata
    30961 ccagagatag attgctcaag agctgggtag ttagaggagc tcttgacgag tgtgtagctc
    31021 ttccagttga taccactgat gccattcaac tctttcataa catcatccaa gttcttgtct
    31081 gctgttacaa agaaggttgc gtccccataa atggctgtgt cttctgtgta tccataaagt
    31141 tttgcagcag tgtgaatgtc tgtaatagct gtgttttcgt aaagttcttg tgagtaggtt
    31201 actgctgact tattatgacc atcaaagagt cccttgattg tcacttcaac tgtttccttg
    31261 gctccttttt cattatctgc atcgtagata ttagagtcca gtttaacctt gtcccctact
    31321 ttccagccgt gtttggctgc caagtccttg tgcaagagga ttttatcctt gtcgtcgttg
    31381 gttaagtgct ctccttcgac tagtttataa gaaccagaga caaacttgtc ttctttagag
    31441 gagtcattga cacctgtaat catcaagcta cttccaaaac gcttggcacg atcagcagtg
    31501 agattcttct tggtttctgg cgtttcaatc aggtcatatc cagtcaaatc tccgatagcg
    31561 ttgatacgtt tgacataaga ctcaatggcc ttgttttcgg tgattttttt gatgtcttca
    31621 cccttgatat tcccagcacc acgaggcgtt ccttggttga cgcgacgatt gatttgcatg
    31681 gagaagctat tggtgatatt tttaaaggtc tcctgagaag ccttggcagt agctcccttg
    31741 attgacaagc cgaccaaact caagctcgcc atgaggagaa taatcaggaa gatgacaatc
    31801 gatttgaaaa acttccttgt aacataggca aatgcgttgt gtaacataga ttccctttct
    31861 agattttgtt ttaatcattc tattaaaata agctcaaatt atttactagt attgcgcgtt
    31921 tcagtcagtt tcttatcctt taattcaagt gtaatatctg acgcttgtgc cacttcttta
    31981 ctgtgagtta cgacaatcac acatttacct gttttctggg caagtgattt gagtagttcg
    32041 acaatatctc cagcagtttt aggatccaga tttcctgttg gctcatcagc tagaataact
    32101 ggagcttctg agaccaaact gcgagcaatg gcaacacgtt gctgttgacc acctgataac
    32161 tggagaacat tccgcttgat ctggctttca tccaaaccaa gctcaagaag tgtattcttg
    32221 cttgcctttt tgttgaccaa tcggatattt tccagcggag aaagataatc tatcaagtta
    32281 taattttgaa agaccaggga aatatggtgc atgcgatggt aagaatagcc cttcttacga
    32341 atatcctctc cttgaaaaag gatagaacct tcaacaggac tatctagacc agcaagtagg
    32401 gacaagagtg tggattttcc tgctcctgac tccccaataa tactgtaaaa ttttccgggt
    32461 tcaaaattat aattgatctg atataggact gcttcagcag tattcttata acggtaggta
    32521 acatcttgta attgtaataa agtcatgatt tctccttctt aactaataga tgataaaatt
    32581 tctttcggtg attttctaaa taagaatagg aaacaaaggg ctacagataa gcaactaagc
    32641 agaactagaa aaacatagga ttctgcaaaa gataagatgc tagttgataa actgcttgct
    32701 ttggctagtg tatcttgtaa gcttgcctga tctccacttg ctagtagagt ttggagtagg
    32761 taagttgtga ttgcgtttcc tgcaacaaat gctggaagca aagctccaag agataccaaa
    32821 actacctcta aacagaattg taggaagatc gagctcttgc cttttccaag tgcaagtaaa
    32881 atccccactt catagacccg ttctctcaac cagagagaca aaaccagaat taaggctcca
    32941 gctcctgcta tcaacatccc ataaaggaag atggtcagga aggtttggaa agttgcaact
    33001 gagtctttga tttgttcaaa agccttgttt tccttttcga cttggtagcc ttgattttcc
    33061 aaggccaagt tttctacctg cttcatgagt ccgtccattt ccttaggatt ttctacatag
    33121 aagcgtgctg cactgacttg agcttcacta ttgcccaaaa gggtttggct actttcatag
    33181 tctgtaaaga cttgattttc actgaagtca gaagacaagc ctgtgaattt ctcttgtttt
    33241 ttaccagaaa agatgccgat aatctcaaac tctactgttt gtccttttcc agattcagac
    33301 tgaccagcat ccaagccaat cttgtcatga agcgaaagac cgttcttctt agccaattct
    33361 tcgtggataa ggattttctt ggaatcccct ttttgaaggt gtcgcccttc ttttagattg
    33421 aaagccgaac tggtaaaggt tacatccttg gatgaatcct caagagccgt taagctaacc
    33481 aagttattgt ctgcagctga taaatcatca cgctccacgc tctgctcgcc agtcactgct
    33541 tccttgtctt ttagttttgc gaccgtctca agttcaggag agacattttc cagcccctta
    33601 atcttgctta cagatgctag gtctgacaac ttgaatgtct gaccattctc tatcttctta
    33661 atagaaaaag atgtattgag tgatttataa agattgcttt ctactgtttt gttggacttc
    33721 atcagagtca aacaggctga aattccggcc aataagacca ataaaatcag aaataaaata
    33781 aaacttctca gtcgctttct gctgacataa gcccaagatc tttggattgg attcatttgt
    33841 cacctccata tttgtaagac tattataaaa cccaaatatg aaatgtttat gaaatacgaa
    33901 aaaaaaatat cgagtagggg ataatctcta gcccctctca caccacc
//
LOCUS       DAVKMU010000019        33844 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKMU010000019 DAVKMU010000000
VERSION     DAVKMU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.019"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(59..295)
                     /locus_tag="VW343_001049"
     CDS             complement(59..295)
                     /locus_tag="VW343_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9755614.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(308..781)
                     /locus_tag="VW343_001050"
     CDS             complement(308..781)
                     /locus_tag="VW343_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755615.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(799..984)
                     /gene="briC"
                     /locus_tag="VW343_001051"
     CDS             complement(799..984)
                     /gene="briC"
                     /locus_tag="VW343_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9755616.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(1143..1910)
                     /locus_tag="VW343_001052"
     CDS             complement(1143..1910)
                     /locus_tag="VW343_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9755617.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKHAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(1907..2773)
                     /locus_tag="VW343_001053"
     CDS             complement(1907..2773)
                     /locus_tag="VW343_001053"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9755618.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(2810..4177)
                     /gene="accC"
                     /locus_tag="VW343_001054"
     CDS             complement(2810..4177)
                     /gene="accC"
                     /locus_tag="VW343_001054"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9755619.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(4189..4611)
                     /gene="fabZ"
                     /locus_tag="VW343_001055"
     CDS             complement(4189..4611)
                     /gene="fabZ"
                     /locus_tag="VW343_001055"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9755620.1"
                     /translation="MIDIQEIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(4608..5093)
                     /locus_tag="VW343_001056"
     CDS             complement(4608..5093)
                     /locus_tag="VW343_001056"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9755621.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(5096..6331)
                     /gene="fabF"
                     /locus_tag="VW343_001057"
     CDS             complement(5096..6331)
                     /gene="fabF"
                     /locus_tag="VW343_001057"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9755622.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(6353..7084)
                     /gene="fabG"
                     /locus_tag="VW343_001058"
     CDS             complement(6353..7084)
                     /gene="fabG"
                     /locus_tag="VW343_001058"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9755623.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(7118..8038)
                     /gene="fabD"
                     /locus_tag="VW343_001059"
     CDS             complement(7118..8038)
                     /gene="fabD"
                     /locus_tag="VW343_001059"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9755624.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(8031..9005)
                     /gene="fabK"
                     /locus_tag="VW343_001060"
     CDS             complement(8031..9005)
                     /gene="fabK"
                     /locus_tag="VW343_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9755625.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(9124..9348)
                     /locus_tag="VW343_001061"
     CDS             complement(9124..9348)
                     /locus_tag="VW343_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9755626.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(9408..10382)
                     /locus_tag="VW343_001062"
     CDS             complement(9408..10382)
                     /locus_tag="VW343_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9755627.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(10382..10816)
                     /locus_tag="VW343_001063"
     CDS             complement(10382..10816)
                     /locus_tag="VW343_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9755628.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(10910..11695)
                     /locus_tag="VW343_001064"
     CDS             complement(10910..11695)
                     /locus_tag="VW343_001064"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9755629.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            12064..13428
                     /locus_tag="VW343_001065"
     CDS             12064..13428
                     /locus_tag="VW343_001065"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9755630.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            13431..13802
                     /locus_tag="VW343_001066"
     CDS             13431..13802
                     /locus_tag="VW343_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9755631.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            14016..15290
                     /gene="serS"
                     /locus_tag="VW343_001067"
     CDS             14016..15290
                     /gene="serS"
                     /locus_tag="VW343_001067"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9755632.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDASVTIPEALRPYMGGAEVIKP"
     gene            complement(15315..16214)
                     /locus_tag="VW343_001068"
     CDS             complement(15315..16214)
                     /locus_tag="VW343_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9755633.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(16324..16872)
                     /locus_tag="VW343_001069"
     CDS             complement(16324..16872)
                     /locus_tag="VW343_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9755634.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(17090..18412)
                     /locus_tag="VW343_001070"
     CDS             complement(17090..18412)
                     /locus_tag="VW343_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9755635.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(18503..18591)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(18755..21091)
                     /locus_tag="VW343_001071"
     CDS             complement(18755..21091)
                     /locus_tag="VW343_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9755636.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRKVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(21195..21743)
                     /locus_tag="VW343_001072"
     CDS             complement(21195..21743)
                     /locus_tag="VW343_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9755637.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQIYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(21740..22042)
                     /gene="zapA"
                     /locus_tag="VW343_001073"
     CDS             complement(21740..22042)
                     /gene="zapA"
                     /locus_tag="VW343_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9755638.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            22129..23013
                     /locus_tag="VW343_001074"
     CDS             22129..23013
                     /locus_tag="VW343_001074"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9755639.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISNPISLEE
                     KAEGKYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQG
                     LNFCAKLHFKNTEKAKNA"
     gene            23025..23639
                     /gene="lepB"
                     /locus_tag="VW343_001075"
     CDS             23025..23639
                     /gene="lepB"
                     /locus_tag="VW343_001075"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9755640.1"
                     /translation="MNLFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            23767..26133
                     /locus_tag="VW343_001076"
     CDS             23767..26133
                     /locus_tag="VW343_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9755641.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSSISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(26181..27464)
                     /locus_tag="VW343_001077"
     CDS             complement(26181..27464)
                     /locus_tag="VW343_001077"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9755642.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(28308..29444)
                     /locus_tag="VW343_001078"
     CDS             complement(28308..29444)
                     /locus_tag="VW343_001078"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU9755643.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(29506..29943)
                     /locus_tag="VW343_001079"
     CDS             complement(29506..29943)
                     /locus_tag="VW343_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9755644.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(29945..31900)
                     /locus_tag="VW343_001080"
     CDS             complement(29945..31900)
                     /locus_tag="VW343_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9755645.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(31924..33693)
                     /locus_tag="VW343_001081"
     CDS             complement(31924..33693)
                     /locus_tag="VW343_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU9755646.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPIANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFFVA
                     ALILHADKSTEDSLEAAQEATQAAKAQSKGQLVSTSVDAVISTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT        10041 a         7624 c         6384 g         9795 t
ORIGIN      
        1 caacaataaa aagaaaaagt gtttgataga tatcaaacac ttttttcttt gcctcccact
       61 atctaaaaaa atgataatag atataattgt aaacaaaaat ccagataggt tttgcatgat
      121 tgagaaagtt aaaaaaacta tggcagagaa tcgttaatct cagattgtcg gtagaacgat
      181 aaacaagggc aaaaaagaaa ccaatcagac tataatataa taaactaatt ggatctctgt
      241 gagatagtat caaatggcta atcccaaaga tgatagcaga taggataaca tccaaatagt
      301 acttggacta gggaaagaag gtattcataa aataccctct atcaagagtc tcctcaaaaa
      361 caggaccgat gattacaggc aggacaaaag ataagatagt cgataaaaag gttggttgtc
      421 catttgaaaa aagcacggta aaatactcat catgaatatt cctatgatta atcaaatgag
      481 catagcgtgc ccaaaaatta ccgagaatct gataaaccac ataagttgca aataagtaga
      541 agacaaatga ccagttccag ctctttttct caaagataaa gagcatcttt ttctttttta
      601 acctccaaat taatagaagg aaacttccca ctaatcccat tgttaaaata agagaataga
      661 catcagctcc taaccctaaa atgatcgtca catacaatcc aattgtttgt ggtaaatagg
      721 tagatagtaa aataataagc aaaaatattc caaattgtct tagttttttt gtgtttctca
      781 tcgtactttt ttgaaagatt accctgctcg gaagccgtac ttccaagcat ctatataaga
      841 attaagtgcc ccttgcctca tatagggagc aaattctcta taatataacc atctactata
      901 tccatcttcc caaacagcaa gaccacctga agtttgctcc aagtcctcag ttgaaagaac
      961 tgtaaatgta tttgtacctg tcattgcaag taccttctta aaatagattg ttgtaggctc
     1021 acatttatag tatatttctt tttttgtcta ttttatagcc catctcctca actggcaatt
     1081 tttcgacctg aattacattt ttccataaaa aatgagacct ttctagtctc atttagtcat
     1141 tcttagtatt ttctaaatcg ttgatagcgt tcttccagca actcttctag cggtttttgt
     1201 gaaagtctag ccagctccgt ttggagttct tttttgacac tcttaatcag ttctttacta
     1261 gaaagtccta tttcagaaat caccttatcc accacgtcca tttctaacag ttcatgcgaa
     1321 gtgattttca tcagttctgc tgcttccatg gcgcgagtac cgtccttcca taaaatggaa
     1381 gcaaagcctt ctggactgag aatggcatag atagaatttt ccagcatcca gacacggtcc
     1441 gcgacagcta gagccagagc cccgcctgaa ccaccttcac cgataataat ggcgataata
     1501 ggaactttca ggtcactcat ttccatgaga ttgcgagcga tagcttcccc ttgaccacgt
     1561 tcttccgctc cgacaccagg ataagcacct gctgtattga taaaggtcac aactggacgg
     1621 ccaaatttct cagcatgttt catcaaccgc agtgcctttc ggtagccttc tggatgtggt
     1681 tggccaaaat tccgtttgag gttgtcttgc aaactcttgc ctttttggat accaaccact
     1741 gttacagctt gatctccaag ccaaccaata ccaccaacaa ctgcaccatc atcacgaaaa
     1801 gaacggtcac catgtaattg gataaattca tcaaaaatgc ctgtcgcaaa gtccaaggtt
     1861 gtcaagcgac tctgctcacg cgcttctctg actatttttg caatattcat ctaggactcc
     1921 ctccatgcaa tctgactagg ctagcaatcg tatctggtaa gtctcttctt ttgacaatag
     1981 catccacaaa gccatgttct aataggaatt ctgccttttg gaaatcctca ggcaagcttt
     2041 cacgaaccgt attttcaatc acacgacgcc cagcaaaacc aaccaagctc tgtggttcag
     2101 ccagaatgat atcgccttcc atagcgaaag aagctgtcac accaccagtc gttggatctg
     2161 tcaaaatggt caggtaaaag agaccagcat ttgaatgacg tttaaccgcc gcagagatct
     2221 tagccatctg catgagactc atgattcctt cctgcatacg ggctccacca gaggctgtga
     2281 ataggacaac tggcaatttt tcgacagtcg catactcaaa caaacgagtg attttttcac
     2341 ctacaaccgt acccatagaa gccatgataa agttagaatc cataatcccg agagccacag
     2401 tctgaccttt aataagagca gttcctgtca caacggcttc atgcagacct gttttttcac
     2461 gcatagatgc cagtttcttt tggtaaccag ggaaatgcaa gggatccttg ctttcaatcc
     2521 ctgtaaacaa ttctttgaag gttcccatat caatcgtcaa agccaagcgt tcttgggcag
     2581 aaatacgaaa ggtatagcta cagtgcggac agatacgttc acttcccaga tccttctgat
     2641 agatggtatg cttacagcct ggacactggg aaaataattc atctggaacc tctggcttag
     2701 cttgaggttt ttccctaacc gaacgattgg gattgattcg aatatactta tcttttttac
     2761 taaatagagc cattgattcc ccttttcggt ttaaactctt aaagtcattt tattcttttt
     2821 cttgatattt aggtaagaag gtttccatca agaaggaagt atcataatcc ccagcaatga
     2881 cattgcgatc tgaaatgagg tcaagctgga aatctgcatt ggtctgcact ccttcaatct
     2941 ctaattcata gagggcacgt tgcattttca tcaaggcgtc aaaacgattt tcgccgtgta
     3001 ctatgatttt ggcaatcata ctatcataat aaggcggaat ggtataacct ggataaactg
     3061 ctgaatccac gcgcaagcca actccaccac ttggcagata gagattagta atcttacctg
     3121 gacttggagc aaagttaaag gctgggtttt ctgcattgat acgacactcg atggcatgac
     3181 cgcgtaggac aatatcttct tgcttaacag acaaaggctg acctgccgca atgcaaatct
     3241 gttccttaac gatatcaaca cctgaaacaa actctgttac tggatgttct acctgaacac
     3301 gagtattcat ctccatgaaa tagaaattgc tacttgcttc atcaagaaga aattcaatgg
     3361 ttcctgcatt ctcatagcca acaaactctg ccgctcgaac agcagcagca cctatttcat
     3421 gacgcagcgt ttttccgatt gcaatcgagg gactttcttc caaaaccttt tggttattcc
     3481 tttgaagaga acaatcccgc tcacccaagt gaatcacatg tccatgctca tcacctagga
     3541 tttgaacctc aatgtgccga gctggataga taacccgttc tatgtacatg gcaccattgc
     3601 cataattggc cttggcctca ctagaggcag tttcaaaggc agaaacgagg tcatctggtt
     3661 tttcaacctt acgaatccct ttaccacctc cacctgctga agccttgagc ataacaggat
     3721 agccaatttt ttcagcaaca atcaaagctt cttcagagtt atgcacttct ccatctgaac
     3781 ctggtataac aggcacacct gctttaatca tctgagcacg cgcattgatc ttatccccca
     3841 tcatatccat aacatgacca gatggaccga taaacttgat acctacttct tcacacatgg
     3901 tcgcaaattt ggaattttca ctgagaaatc caaaaccagg gtgaatagct tctgcctcag
     3961 tcaagactgc agctgataga actgcattaa tattgagata agactctgtt gccttgccag
     4021 gaccaataca aactgcttca tctgccaaaa gcgtatgaag agcttcctta tcagcagttg
     4081 aataaaccgc taccgtcgca atccccaatt cacgtgccgc acggataata cgaaccgcaa
     4141 tttcaccacg attggcaatt aaaatttttc gaaacatgga gaacctcctt agttcccaat
     4201 tgcaaaagta aggataccac tggctgcaag cttgccatcc acttcagcct ttgcttcaac
     4261 cacagctatg gtgccacgac gttttacaaa agtcgctgtc ataaccaatt ggtcgcctgg
     4321 tacaacttgc ttcttgaact taaccttgtc cataccagcg taaaagacca gttttccttt
     4381 attttcaggt tttgataact ccaacacacc agcagtttgc gccaaggctt ccataatcag
     4441 aacacctggc ataactgggt attgaggaaa gtggccgtta aagaaaggtt cgttaatggt
     4501 cacatttttg atagcaacaa tggtatcctc gctcacttcc aagacacggt ccactagaag
     4561 cataggataa cggtggggaa gagcttcttt gatttcttga atatcgatca tttgatacgt
     4621 accaatcctt taccaaactc aaccatttct tcgttagaga cgagaatttc cgttaccaca
     4681 ccatccttag gagctgggat ttcattcatg actttcatgg cttcgataat taccaatgtt
     4741 tgaccttttt tgacactatc accaactgta acgaaggcag gtttatctgg tccagcagcc
     4801 aagtaaacca ctccaacaag tggactctct acaagatttc cctcagtagc cacacttgct
     4861 tcagctggag ctggaacttc ttctgctaca gtctctgctg gagcagatgt aggagctact
     4921 ggactcggtg ttgctagaac gggtgctgga gcgacttgag ttgcaacttc aggcacaggt
     4981 ctcgcttcat tcttgctaaa ctgcaactca tccgtcccat ttttataaga aaattctctc
     5041 aaacttgact ggtcaaattg agtcatcaag tctttaatat cgtttaaatt catacttatc
     5101 tattctccca acgtttgaaa gcaagaactg cattgtggcc tccaaaacca aaagtatttg
     5161 aaatagcgta tggaatttct ttctccaagc cttgtccata aacgacatta gcttcgatat
     5221 aatctgatac ttcacttgtc ccagctgtca ttggtacaaa gttatggcgc atagcttcga
     5281 tggtgacgat agcttctact gcacccgcag cccccagcaa atgtcctgta aaagacttgg
     5341 ttgatgatac aggtacttcc ttaccaagaa cagctacgat agctccactt tctccttttt
     5401 cattggcagg agttgacgtt ccgtgagcat tgacataggc tacttgctct ggagaaatct
     5461 cagcttcttc caaggctagt ttgatggcct tgatagctcc ctgaccttct ggatgtggag
     5521 aagtcatgtg gtaggcatca caagtatttc cgtaaccaac cacttcagcc aggatagtag
     5581 ctccacgttt ttcagcgtgt tcaagacttt ctaaaaccaa catccctgaa ccttcaccca
     5641 taacaaaccc attgcgatcc ttatcaaatg ggatcgaagc acgagttgga tcctctgtag
     5701 tagagagagc tgttaaggct tggaaaccag cgatggcaaa aggtgtgata gaagcttctg
     5761 ttcctcccac caacatcaca tcttggaaac caaacttaat ggagcggaag gcatccccaa
     5821 tcgcatcatt tgatgaagag caggcagtat tgatagattt acaaacaccg tttgcaccaa
     5881 aacgcatggc tacattccca gaagccatat ttggtaaagc ttttggaaga gtcattggtt
     5941 tgacacgttt gggtcctttt tcatgaaggc gaagtacctg atcttcaatt tccttgattc
     6001 caccaatacc agatgcaacg ataacaccaa aacgatccct attaagagcc tctacatcaa
     6061 gattggcatg atttacagcc tcttgggctg catacaaggc atataaagaa tagttatcaa
     6121 aacggttggt atcttttttt acaaagtatt tatcgaacgg aaaatcttgg atttctgccg
     6181 cattatgcac atcaaagtca ctatgatcaa attttgtaat gccaccaatg ccgattttcc
     6241 cagttgctaa actattccaa aattcttctg gtgtatttcc gattggagat gttactccat
     6301 aacctgttac cactactcga tttagtttca ttcttttcac ctctagcttt cgctacatac
     6361 ttaagccacc atcaatggca atcacttgac cagttagata atcttggcct gctaaaaata
     6421 ctgtcaaatc tgcaacctgc tctgcctgcc caaattcttt catcggaatc tgagctagtg
     6481 tagcttcctt aatcttatct gataagatag ctgtcatatc agactcaatc attcctggag
     6541 caatcacatt gactcgtata ttccgactag cgacctcgcg tgccacagac ttggtaaagc
     6601 caatcaagcc agccttagaa gcagcatagt tagcttgacc aatattcccc atcaaaccaa
     6661 caacactaga catattaatg atagcacctt ctctggcttt catcatcggt ttcaagactg
     6721 attgtgtcat attaaaggca ccagtcagat tgaccttgag cactttttca aaatctgctt
     6781 ctgtcatctt gagcataaga gtatcttggg taatccctgc attgttgacc aaaacatcta
     6841 ctgaacccag ttctgcaata gcttgatcaa tcatacgctt agcgtctgca aaatctgata
     6901 catctcctga aatgggaacc accttgatac catagtttga aaactcagcg agcaattctt
     6961 ctgagattgc cccacgactg tttaagacaa tgttggctcc tgcttgagca aacttgtggg
     7021 cgatggcaag accaattcca cgactcgaac ctgtaataaa gatattttta tgttctagtt
     7081 tcattttttt cctttcaaaa cttctactta ttttagtcta tttttctaaa agtgctacta
     7141 aactcgcttg atcttccaca tgagctaagt gagcagtttg atcaattttt ttaacaaaac
     7201 ctgacaagac tttccccggt ccaatctcga taaagttgct tatgcctgct tcttgcatga
     7261 ccccaatact ttcatagaaa cgaacgggtt ccttgacctg acgcgtcaag agctgagcaa
     7321 tgtcctcttt ttgcatcaca gcagcttctg tattgccgac taggggacaa gtaaaatctg
     7381 aaaaacttac ctgagctaga gtttcagcta gtttctggct agctggctca aggagagcgg
     7441 tgtgaaaggg acctgacacc ttaagaggaa tcaagcgttt ggcacctgct tcttgcaaaa
     7501 gttcaaccgc tcgatcaact gcaaccactt ctccagcaat gacgatttgt gcaggtgtgt
     7561 tatagttggc tggagtaacc actccaagtt cagaagcttt ttgacaggct tcttcaatga
     7621 cctctactgg cgtattgaga actgctacca tcttgccaga gtcagcagga gccgcttctt
     7681 ccatataggc tccacgctta gctaccaagg caaccgcatc ttcaaaatcc aaggcgccgc
     7741 ttgccaccaa ggcagagtat tctccaagag acaaaccagc aaccatatca ggctgatagc
     7801 ccttttcttg caataaacgg tagatagcaa ccgaagtcgc tagaatggct ggttgcgtat
     7861 agcgggtctg attgagtttg tcttcttccg tatcgatgag ataacgcaaa tcataaccta
     7921 gcacctgact cgctcgatca atcgtttctt taacaatcgg atactgatca tagaaatccc
     7981 gtcccatccc tagatactgg gcaccttgac cagcaaataa aaaggctgtt ttagtcattt
     8041 cttacaactc ctgtccagcg agaggcttct tcttgaattt tcttagcggc tccgtaatac
     8101 aaatctttta ggatttcttc agctgtttct tctttagaaa caagccctgc gatttgacct
     8161 gccatgacag agccaccctc cacatcaccg tgaacaactg ctttggctag ggcaccggct
     8221 cccatttgtt caaagatttc taaatcagga tcttcctgct taaaggcatc tttttcagcc
     8281 agttcaaaat ctctagtcaa ctgattttta atagcacgaa cagcatgacc aaagtgctga
     8341 gctgaaatcg tagtgtcaat atcccttgct tttaaaattt tctccttgta gtttggatgg
     8401 gcattcgact cttttgcaac tacaaaccgt gtccccacct gtacagcctc tgcacctagc
     8461 ataaagccag ccgcagcacc ttcaccatcc gcaattcctc ctgcagcaat aacaggaata
     8521 gatacagctg tggctacctg tcgcaccaag gtcatggttg ttaatttacc gatatgcccc
     8581 ccagcttcca ttccttctgc aataacagcg tctgcaccga ttttttccat gcgtttagct
     8641 aaagcgacac taggaacgac aggaataacg attatcccag cttcatggaa acgttccata
     8701 tacttgcttg gatttcctgc tcctgttgtg acaactttaa caccttcttc aataacgaga
     8761 tccacgatat cttccacaaa gggagataag agcatgatgt tgaccccaaa gggtttatca
     8821 gtcaatgatt tgattttatc aatattggcc ttgacaactt ctttcggggc atttccccca
     8881 ccgataattc ctaatcctcc agccttggaa acagcccctg ccaaatcacc atcagcaacc
     8941 caggccatcc ctccttggaa aataggatag tcaatcttca ataattctgt aatacgcgtt
     9001 ttcatagtgc ctccaacctt ccttgcttac gtaatagttc gatttcacca taatttgaca
     9061 gtcaaactat tacctaaaca agagggagtg ggtttctccc tactccttct actaatattc
     9121 tgcttatttt gcttgctctt caacgtaagc aaccaagtca ccaactgttt tcaagtcatt
     9181 ttctgcttcg atttggatat caaaagcatc ttcgatttct gagattactt ggaacaagtc
     9241 caatgaatct gcgtccaaat catcaaaagt tgattcaagt gttacttctg atgcgtcttt
     9301 tccaagttct tcaacgataa tttcttgtac tttttcaaat actgccatga taggactcct
     9361 ttaaaataaa tagttttttt ataacaatgt gttcaccaca tgattaccta aattgtaaga
     9421 atgagcgtgc cccaggtcaa gcctccaccg aagcctgata gaagaacagt ctggctacca
     9481 tctaaaggga tgagaccttg ttctacacac tctgaaagta aaatcgggat actggctgca
     9541 ctggtattgc catattccat catattggct ggaagtttgg ctcggtcaac accaattttt
     9601 ctagccatct tatccaaaat acggtcattg gcttgatgaa gtagcagata atccaagtct
     9661 gtcacctcta taggagattc atcaatagtc tgcttgatag acttggctac atctcgaatg
     9721 gcaaaatcaa agactgtgcg tccatccatc ttcaaaaatg aatctgcact ttcttgatct
     9781 gaaaatggag aatgcaaacc tgaatgccca taagttaaac actcgctgcg acttccatcg
     9841 ctattgagac tctcagctaa gaaatgctct tgctcgctag cttctaacaa gacaccacca
     9901 gcaccatctc caaacaacac agctgttgat cgatccgacc aatcgactgc cttagagagg
     9961 gtttcactac caatcaccaa gcctttttga aagcgaccag aagcgataaa cttttcagca
    10021 gttgaaagag caaatacaaa tccactgcaa gccgcggtta agtcaaaagc aaaggcttta
    10081 ttagcaccaa tattagcttg aacacgagca gctgtagagg gcatcatcga atctggagta
    10141 atggtagcta ggatgataaa atccagttct tctcctgtta ttccagcttt tgccatcagt
    10201 ttcttagcaa cctctgtagc caaatcactg gtagattctg ttcttgaaat atgcctttgt
    10261 cgtattcccg ttcgacttga aatccactca tcattggtat ccataatctg agccaagtcg
    10321 tgatttgtaa ccacttgctc tggcacataa tgagcaacct gacttatttt tgcaaaagcc
    10381 attatttcaa atcctccaaa aattggtaaa gattagtcaa gcctttaccc atgacagcaa
    10441 tttcttcctc gctcatgcca tcaataattt tttctaccat ggccttgtgg aagcgtttat
    10501 gcagtctatg aatcaagcga cccttctttg tcaaatgcag atgcaccaca cgacgatcct
    10561 gttctgaccg aactcgctca atgtagccct tacgctctaa attgttcaaa cttgtcgtaa
    10621 cagttccaag agttaccatc aactcttttg acacttgact tggagtcaca tctggagcct
    10681 taccgatgac atcaatcgta tgcatttctt tgatggagat atccttaaaa cgactacctc
    10741 tcaagttcac ttcctcaatt acaaggacat tgttaaatat agatgttaaa tattcattga
    10801 ttcgttggta gtccattttt catatccctc cttcttcaaa aaacttttac aatcaaaaca
    10861 tttgacagaa agaattatat caaaattcaa agaatgatgc aagtattttt tattttcctg
    10921 taaatttagg tcttcttctc tccgaatgag ctcgcactcc ttctttgaaa tcctctgttt
    10981 gagctaagga tttctgtagg ttcagttcta aagtagcgta accctgccag tctttaaatt
    11041 ggctctccca aaccaacttc ttaatggcag cataggaatt acttgaagca cgtcttaatt
    11101 ttttaagaag ctgttctctc gtcttttcaa gtttttcagc ttcagaaacg cggtaaacca
    11161 acccccattc tagggctttt tctgccgtta aagcctctcc tgtcatagct aattgagcag
    11221 cacgcgtcac accgatactg cggctcaaga gatgaattcc ccctgcatct ggagccaaac
    11281 caacaccaac aaaggcttgg ataaacttag ccttatccgt cgccaaacag aaatctgccg
    11341 caacagccat attcgctgcg gcacctgcaa cagcaccgtc aacttccatt aaaacaggtt
    11401 tggctatttg cttgatttta taagaaatcg tattgaccaa ctctgcgatt tttgtcaatg
    11461 atggaatatc atcctcatcc actgcccgct tcatctctac caaatctccc ccaactgaga
    11521 agaccttccc attggcattg attaagataa aatgcacagc tggattttct tctgccaaag
    11581 tcagagcttc taaaatctcc tcacacatgg gaatatgaaa accatttgcg acctcaggac
    11641 ggttcaaggt aaggattgcc aagtcctctt caagctgata aataatgtgt tccatcaggg
    11701 ctccttttta ttttataagc agtttatact cttcgaaaat ctcttcaaac cgcgtcagct
    11761 tccatctgca acctcaaaac agtattttga gctgacttcg tcagtcttat ttacaacctc
    11821 aaagcagtgc tttgagcagt ctgcggctag cttcctagtt tgctctttga tttcattgag
    11881 tgttaaatta tttcactgtc aaagtatatc ttttttagga gcagaaagca agaaaaaatc
    11941 gactgaaagt gtctcagccg ataagaaagg ctcgctttag gctagaaagc atcttatttt
    12001 cttcattgaa aaaagccttt ctttctgcta taatcgtata aaatacttac tttaggagtt
    12061 cttatgaaag ttgttaaatt tggaggtagt tctcttgcct ctgctagtca attagaaaaa
    12121 gttttaaaca tcgtcaaaag cgattcagag cgtcgttttg tagtcgtttc tgcgcctggt
    12181 aaacgcaatg ctgaagatac taaggttacg gatgccctga ttaaatacta ccgcgactat
    12241 gttgcgggta acgatattag caagagccaa agctggatta tcgaccgcta tgctgctatg
    12301 gttagtgaat tgggactaaa accagctgtg ctagaaaaaa tttctaaaag tattcacgcc
    12361 ttggccactc ttcctattga agaaaatgaa tttctctacg atactttcct agcagccggt
    12421 gaaaataaca atgccaaatt gattgctgcc tactttaacc aaaatggtat cgatgcacgc
    12481 tatatgcacc ctagagaagc tgggattgtg gtcacaagtg aacctggtca cgctcgcatc
    12541 attccatcaa gttatgacaa gattgaagaa ttgacaaaca ccaatgaagt ccttgtcatt
    12601 cctggtttct ttggtgtcac taaggaaaat caaatctgta ctttctcacg tggaggttct
    12661 gatattacag gttctatcat tgctgctggt gtcaaagctg acctctatga aaactttacg
    12721 gacgttgatg gtatctttgc agcccaccct ggtattatcc accaaccaca ctcgattcct
    12781 gagttgacct accgtgaaat gcgcgagttg gcctatgcag gcttctcagt ccttcatgac
    12841 gaggctcttc ttcctgccta ccgtggaaaa attcctctgg ttatcaagaa taccaacaac
    12901 cctgaccatc caggtactcg tatcgttcta aaacacagta atgatgaatt tccagttgtg
    12961 ggaattgctg gtgactcagg ctttgtcagc attaacatgt cgaaatacct catgaaccgt
    13021 gaggttggat ttggccgcaa ggttctgcaa atcctggaag aacttaacat cggttgggaa
    13081 catatgccaa caggtatcga cgatctttct atcattctcc gttctcgcca actaactccc
    13141 atcaaggaag aagaaatcct gcgtcagttg gttcaaaagg ctaaagtaga tcatgcagaa
    13201 atcgaacacg acctttctat cattatgatt gttggtgaaa aaatgaagag ccacatcgga
    13261 gtgactgcta ctgcgacacg cgctctatct gaaaacaaaa tcaacatcca gatgatgtct
    13321 caaggttcta gtgaagtttc tattatgttt gttgtcaata aagaccaaga gaaagcagca
    13381 attaaagccc tctacaatgc cttttttgga gaaagtaagg aagactaagc atggcccaat
    13441 cacttaacaa aacagtactc ctcagtacga caggcacttc ctacctctct atcgctggga
    13501 aagttgggaa attccttgtc ggagatcagg ctttggaatt ttacccagat gtcaatgtcg
    13561 aacaatttat ccagattcct tggagccata tcaatcagat tggagccaat gtcactggac
    13621 gcaaaatcag ccgccacttc gaagtcttta cagacaaagg aaaattcctc tttgcttcaa
    13681 aagactcagg tgccattctc aaaattgcac gcgaaaaact aggcaatgac aaggtcgtca
    13741 aacttccgac cctgattcag acaattagcc aaaaatttaa aaatctattt gcaaaaaagt
    13801 agaaacttta gtataatcat agcaacggat aagtaggtta tcttcttctt tcagaaagtc
    13861 tgcgggtact gcgagcagat aggagggata ggtgaaattc taccgttagt aacaattaca
    13921 tacacaatca agtgcacacc tgtgaagttg gatggaaccg tggccctgcc actccaacgt
    13981 tttgtcaggc gtgctttttt cataaaggag ttcttatgtt agatatcaaa cgtattcgta
    14041 cagattttga agctgtcgca gaaaaattag ctacacgtgg tgtagatgct gctgtcttga
    14101 atgaaatgaa agaaatcgat gctaaacgtc gtaacatctt gattaaggtt gaaactctca
    14161 aagcagaacg taacacagtt tctgctgaga ttgcccaagc taagcgcaac aaggaaaata
    14221 cagatgacaa gattgctgcc atgcaaaatc tatctgctga ggttaaagcc ttggatgctg
    14281 aattggcaga aatcgatgct aaattgacag aatttacaac gactcttcca aatatcccag
    14341 ctgacagcgt tcctgttggg gctgacgaag acgacaatgt ggaagttcgc cgttggggta
    14401 ctccacgcga gtttgacttc gaacctaaag ctcactggga tctcggtgaa gaccttggta
    14461 tccttgactg ggaacgcggt ggtaaggtaa caggcgctcg cttcctcttc tataaaggcc
    14521 tcggtgctcg tttggaacgt gctatctaca actttatgtt ggatgaacat ggaaaagaag
    14581 gctatactga agtcatcaca ccttacatag tcaaccatga ttctatgttt ggtactggtc
    14641 agtatccaaa atttaaggaa gatacttttg aactcagcga taccaacttt gtcttgattc
    14701 caactgctga agttcctctg acaaactact accgtgatga aatcttagac ggcaaagatc
    14761 ttccaatcta cttcactgcc atgagtccgt cattccgttc tgaggctggt tctgccggtc
    14821 gtgatacgcg tggcttgatc cgtttgcacc aattccacaa ggttgaaatg gtcaaatttg
    14881 ccaaaccaga agaatcttac gaagaattgg aaaaaatgac agccaacgct gaaaacattc
    14941 ttcaaaaact caaccttcca taccgtgtcg ttgctctctc tactggagat atgggcttct
    15001 cagctgcgaa gacttacgac ttggaagtgt ggattccagc acaaaacaat taccgtgaaa
    15061 tctcaagctg ttcaaacaca gaagatttcc aagcccgtcg tgcccaaatc cgttaccgtg
    15121 atgaagcaga tggcaaggtg aaactccttc ataccttgaa cggttctgga cttgcagttg
    15181 gacgtacagt ggctgcaatt cttgaaaatt accaaaatga agatgcttct gtgaccatcc
    15241 cagaagcact tcgtccatac atgggtggag ctgaagtcat caaaccataa aaaataaggt
    15301 ttagctattt ctagctagac cttttttcgt aaccaaatca gataagcacc tagtacaaag
    15361 aataaaatag ttaggcatat aatggtttca gccaatacca ggtaatccag aaatggaagt
    15421 ttcaaaattc cctgagccat cttgagcgag gtcgctgtga taatggttgg gaaggtgagg
    15481 gctgagaagg ctggttgaaa accttgtttt aaaatgttgg gcagacgagt taaaacaaag
    15541 aaaaagaagg attgagaagc caaaatcatg acaatcaaga cccaagtcgg caggctggtt
    15601 cctcctactc gaactagaga agccaagagt agagagaaag gagcacagta gattccttct
    15661 tgtccaagca aggctagtgg gagtggatgt ttctttaaat cgctataaat aaggggatag
    15721 agatagaagg tcaagagaaa accaaaactc aaggtcgcat aggcaatttc gataatacct
    15781 accagaggat aggtcaaggc agccactgct atccccacat agagaaccgt ccaacttgga
    15841 gtggcatgaa ccctccgccc tggacaagca aacttgatgg taaaaccagc aatcaaggtc
    15901 aaatccaaga gaaatgaaaa ccaccaaatc ccttgtgcta ccaaaggaag ataagagaat
    15961 acgcgaaaga cataggtcga taaaatcatc ccagccatag gaaaggttgc cattcctgac
    16021 aaaagagggg gcttggtcaa ttcttgcttg gtttctttcc aattaaagag atgcagaatt
    16081 agaaagtaaa tccataaaac caaaccagtc agactaaaaa gatgggatag aaccggcaac
    16141 gtatctaaaa taagatttcc agctcctgcc aaacctagca aacaacctga aaatactaag
    16201 gggagttttt tcatcctaac ctccaataat catgttagtt tcagtataac ataaaagcgc
    16261 ttaaatgagg atttaaaaaa acgaagtccg cttatttcag acttcatttt actcagatat
    16321 gaattaggca taaggttgca attctggatt aattggtgta ttagctaagt tgttggcata
    16381 gttacagagg attgctaggc tgacaccaaa aaccacatcc aaggcatttt gttgagtgta
    16441 gccagcttct aaaaactcag acaaggcttc atctcctaca cgacccttgg tattgataac
    16501 tgccaaggta aacttagcta gggtatccaa tttaggatct gtttcaattg gagtacgatt
    16561 gcgaagagct tgaatcaagt catcattcat ctggatttgt ttgatggaaa aggctgtgtg
    16621 acctgcgaca cagaaggcac aaccattggt cacggctgcc gtgatttgca ccacttcacg
    16681 ctcaacgggt gtcaggctgt tgcgacggtg gatagatgag acaatttgat aggcttctaa
    16741 aacagtcggg gcattggcca agagaccgat taggttggga atatagccat tgttgtcttt
    16801 ttctactgtt tcaagaattt ctttcacttc tgctggtgct gactctactg tatggatagt
    16861 aaatgttgtc ataagatacc tcttttctta ttattgacac taatattatt ggaaaatctt
    16921 ataaaatcct gattcctaag tttatctaag ataaagcttt attctctcat aagattttcg
    16981 ttgttatatt agtttatcac acttccaatc acttgcataa tatatattat atatcaggct
    17041 gataaaaatt atttataggc aaaaaaatca cacgagctgt gtgattccat tatttgtcaa
    17101 aatacttttt agtttcagca ataacgactg gcgacaagac caagagggca atcaagtttg
    17161 gcagagccat caaggcgtta acgatatctg cgataatcca gaccatatcc aactcgataa
    17221 atcctcctaa caagaccatg agcacaaaaa ccacacggta gagccagata aagcgaaccc
    17281 caaagaggaa ctcaaaacag cgttctccgt aatagttcca acctagaatc gttgtaaagg
    17341 caaaaagtac aaggaagatg gtcaagaggg caggcccaaa gtgtgaaaag actgttgaga
    17401 aagctgactg agtcaaggca accccattca agtcaccact ccaaactcca gttaccaaga
    17461 tggtcaaacc agttagagta caaatgatga gggtatcaat aaaggttcct gtcatggaaa
    17521 tcaaaccttg ctctactggt tcatttgtct tggcagctgc agctgcaata ggagcagaac
    17581 ccagaccaga ttcgtttgag aacacaccac gcgccacacc attttgaata gccatccgaa
    17641 cgctagcacc agcaaatcca cctaccgcag caaggggact aaaagctgag gtaaagacta
    17701 aagcgattgt gccagggatt tttccgatat taaagaaaat aactgtaaga gttcctaaga
    17761 tataaatgat ggccataaaa ggaacaacag tagttgaaac cttagaaata gacttgagtc
    17821 caccaaagac tgcaatcgct acaaagacag acaagacgag agctgtgatg gctggcgaaa
    17881 tcgtcgttgt attttggata gattctgcaa tcgagttgac ttgggtgaag gttccgattc
    17941 ccaagagagc aaccaatact cctgctactg caaacaaaac agcaagtggt cgccactttt
    18001 ctcccatccc tagaaggata taatgcatgg gacctcccgc tactgcacca tggtcgtcct
    18061 tggtgcggta tttgatggcc aagagtcctt ccgcatactt ggtagccatt ccaaagaaag
    18121 ccgccatcca catccaaaat agagctcctg gtccaccaac cttgatagcc gtcgcaactc
    18181 ctatgatatt tcctgttcca acagttgatg ccaaggctgt acacagagct gcaaaactgg
    18241 atacatcacc atgtccctta tcctggataa aaataagctg aaaagccttg ggcagacgca
    18301 aaacctgcaa gagtcctagc cgaatagtta ggtaaatccc tgttccgacc aataaaatca
    18361 agaggggcgg tccccaagca aaagcatcga ttgatttaag caattctaac atttccttct
    18421 cctatcgttt caaccccaaa agaaagagca catgcaagat acatgtactc tggaatgctt
    18481 agataaatgc taaaaagcgg tctatcctag ctctgtcctt ttacctgaga gtttgagcag
    18541 ttgcctgcct tgccccttcg gtgcctttac ggtctctcca gagttccgtc catttacagt
    18601 catggaaaat caaacgattc cccacttcta ttaaacttca ttcggtgttg gtatttaatt
    18661 gattctaatt ttacaaaaaa tgttggcttt tgtcaatgtg tttattagta aaaattagtt
    18721 caacagtttt tactttataa agtccagaat actgctatcc tttaaaagtg acaatagtcg
    18781 caccactgcc tccagcattt tgtggggcat agccgaaact cttgacatgt ttgtttcttt
    18841 gcaagtattt ggtaactcct tcacggatga ctcctgttcc gataccatgg atgatatcaa
    18901 cttgagccat attgttaagc aaggcttggt cgatgaaggt atctagctca ttcatggctt
    18961 cttcatagcg cttgcctcga agatccagtc tagcttgagg tcctcgccca gaagttcgtt
    19021 tcacaacatt gacctgtttc ttcttgactg gtttttcttg ctgggcttga acaagatcaa
    19081 actctttctc ttccaaggtc atcttaatca agccaacttg ggcttcccag cggccgtcct
    19141 tgagttgact ggtcaaggta ccacgctgac cataactgag aaccacgata tcatctccca
    19201 cctttggagc tcgttttttc ttggcctttt gaaggacctt gtttttagac aagtccactt
    19261 tttcaggagc caattttttc aacttggcct tggcttcaat gatttcgtgg ggcttgagtt
    19321 gggatttact gtggagattt ttgagaatct ggtcactttc acttagggcc atatccacaa
    19381 tctcagcagc ctgttcacgc gccttgttaa gctcggtttc cttttcacga ttaagctcgt
    19441 tgtagagttt ttttagcgca cggttcatct tgagattttc ttgctccacc tcacggatat
    19501 tgtccaagcg tttgcggctt tccagcgtct gctcttctaa ttgctcaatg atacgattga
    19561 cgtcattgtc ctgatcgatc tgctgactgg catctcctac gataacttca gataggccta
    19621 gacgtttggc aatttcaaag gcattacttc ggccaggaac accctgcata aagcgatagg
    19681 tcgggcgaag agttgcagta tcaaactcca tactggcatt ttgcacaaag gctgtctcaa
    19741 taccgtaggc cttgagttct ggatagtggg tcgtcgccat ggtcttgatt tgacgcaggc
    19801 gaaggtcctc cagaatagcc atggcaaggg ctgctccctc ttggggatca gtaccagccc
    19861 ccaactcatc caaaagtaag agtgaatgtt ggttgacctt gccaagaata tccacgatat
    19921 tggtcatatg gctagagaag gtagacaagc tctgctcaat agactgctca tctccaatat
    19981 cagcaaaaat ttcttcaaaa ataccaacac gacttccctt gtctgctaaa atcggcaatc
    20041 ctgactgggc catgacctgt gtcaagccca gagttttgag catgatggtc ttcccacctg
    20101 tattgggacc tgtaatgaca atagctgtta aatcttgacc aaaatagaca tcatttgcga
    20161 cggcattttt gaccaaagga tggcagacat ggagcagttg aatctcttga ttttctgaca
    20221 gctgaggcac gactgcttgt ctttcttgga taaatcgaac cttggcacga atcaagtcca
    20281 gatgaccgat aatccaagca tcattagcaa tctcagccgc atgagggcgg acacgctcag
    20341 aaatttcttg gagaatgcga agcatttcat agcgctcatc tgctcgcaga ctagcaattt
    20401 cttcgctcag tttgactacc tcacggggtt cgatatagac ggtgtttcca ctagcagaaa
    20461 tatcatgaac gacacctgca atcttattgc ggtaggtgtt tttgactggt aaaaccttac
    20521 ggccatttct gctagcaaca attccttccg tcaacatctg cgctttttgc ttgagcaagt
    20581 cttgtaaaac atcgcgtacc tgactctcgc tatcatgtat ttttcgacgg attcgcgcca
    20641 attcttcact ggcaaaattt tcaatgaaac ccgcatcatt aaaggcctga agatttcctt
    20701 gtaattgcgg aaaatcatgt aatttctcaa accaaagggc taattcttcc aagctgacat
    20761 tttccagatt ggcgtaaaaa ctttgaagtt ctcggctggc aagaagcacg cgtttcaaga
    20821 gtaggaactc ctcgatattg agatccgctc ccatctccaa cctcttgcag actcctgcaa
    20881 tttccttagt tgagagaata gtaaaatgcg gttgctcgac gaaaagagcc tgcatttcct
    20941 tcatctcagc aaaagcctgt ttgattttat ctgctttggc agtcggagcc agttgtctca
    21001 attgctccaa gccctgctcg gtcaacaaat gaggctcaaa caaggccttg accttatcga
    21061 actctaatgt ttctaatatt ttcttattca tctttatttc cttgtctttg aatgtctagg
    21121 tgtggtaact ttttacattc tgatagattc aagtcaaatc tgtaaacaaa gggctaggaa
    21181 aactcctgcc ctttttatcc gattaaattt gtcacccaga tttgtttgag ccaactggtt
    21241 gttatcggta tgctctggat gatgtgtttt gcgacgatac tcttttcaag aggattttgt
    21301 ataactgcca tggggatggt cgccaagatt gtcaaggcca tttgcaagac aaataaggtc
    21361 accaacatgg acaagatacc tgctgaaact tggaacaact taccacccag ttttttgcta
    21421 ggaatcaagt gtaagagaag accaagcaaa cgaccaatgc tatagacaat cccaaataca
    21481 agcaagtagc cgatacctgc ataaaagacc ttatccagct gaaagagttg atccgatggg
    21541 aaaaagaaag tcccctgacc ttcctgcgaa tttgcataag ggagcaataa atggaattgc
    21601 tctccaagcc ccttataaaa ctggccagcc ataaaagccg atgccatggc tgaaatcagg
    21661 taataaatct gtaagagcag gcctctccga tagccgatat aaaatcccca aaccaagacc
    21721 aatagaagaa ggaatgaaat cataaggaat cctcaatctt gctctgttct tgcttacaag
    21781 tcacaagctt gtgacggagt tcttctagct cttgctcctt atcgtcaaat tcaatctcac
    21841 ggctgagctg agttgataaa cagttgactg ccaacaaaag agcgattgtt tcatcatctg
    21901 cgctaggcat ttgttcttta attgcttggt atttttctgt cgcaacctta gcgatttcct
    21961 ccataaaaag gttatcatgt tcacttgtca aggttaacga ttttttcccg aatgtaaatt
    22021 tgaatcgatt tagatttgcc ataaaattca cctcacgata ttataccaaa attcgctaat
    22081 tttgtcagtt tttacaaatt ttactgcttt tgtggtacaa tagaaactat ggcaagtatc
    22141 acactcacac caagcgaaaa ggatattcag gcttttcttg aacactatca aaccagtctg
    22201 gctcctagca agaatcccta tatccgctac tttttgaaac tacctcaagc aacggtttct
    22261 atctatactt ctggaaaaat cttgcttcag ggtgaagggg ctgaaaaata cgccagtttc
    22321 tttggctatc aagctgtaga gcaaaccagc ggacaaaatc ttcctttgat tgggacagat
    22381 gaggtaggga atggttccta ctttggtggg cttgcagttg tggcttcctt tgtcacacct
    22441 gaccagcatg ccttcttacg aaaactcggt gtgggagatt ctaagactct gaccgaccaa
    22501 aagattcgtc agattgctcc tattctcaag gaaaaaattc agcaccaggc actccttctc
    22561 tcacccagca agtacaacga ggtcatcgga gaccgctaca atgctgtttc ggttaaggtt
    22621 gccctccata atcaggctat ctatctcctc cttcaaaaag gtgttcagcc tgagaaaatt
    22681 gtgattgatg cctttaccag tgctaaaaat tatgacaagt acttggcaca agagaccaat
    22741 cgtttcagca atcctatcag caatcctatc agcttagaag aaaaggctga gggcaaatac
    22801 ttggctgtcg cagtttcttc tgtcattgcg cgtgatctct ttctggaaaa tcttgaaaat
    22861 ttgggacgag aactgggtta tcagcttcca agtggagctg gaacggcttc tgacaaggtg
    22921 gctagccaga ttttgcaagc ctatggtatg cagggactca acttctgcgc caaattgcac
    22981 tttaaaaata ctgaaaaagc gaaaaacgct tagaaaggta agttatgaat ttatttaaaa
    23041 atttcttaaa agagtgggga ttattcctcc tgattctgtc attactagct ttgagccgta
    23101 tctttttttg gagcaatgtt cgcgtagaag gacattccat ggatccgacc ctagcggatg
    23161 gtgaaatcct ctttgttgtt aagcacctcc ctattgaccg ttttgatatc gtggtggccc
    23221 atgaggaaga tggcaataag gacattgtca aacgtgtcat cggaatgcct ggcgatacca
    23281 tccgttacga aaatgataaa ctttacatca atgacaaaga aacggacgag ccttatctag
    23341 cagactatat caaacgcttc aaggatgaca aactccaaag cacttactca ggcaagggct
    23401 ttgaaggaaa taaaggaact ttctttagaa gtatcgctca aaaagcccaa gccttcacag
    23461 ttgatgtcaa ctacaacacc aactttagct ttactgttcc agaaggagaa taccttctcc
    23521 tcggagatga ccgcttggtt tcgagcgaca gccgccacgt aggtaccttc aaagcaaaag
    23581 atatcacagg ggaagctaaa ttccgcttct ggccaatcac ccgtatcgga acattttaag
    23641 agacctaaga ggccgagaat caccaatctc agcctcttct tctatcgtga gaaaatgatt
    23701 ggtaactatc taaacttacc agaacagaaa cacctcaact ctcacctatt catgcaaagg
    23761 aattttatgg aagtttattt ttcaggaacc attgaacgga ttatttttga aaatcccagc
    23821 aatttttatc gcatcctcct cctagaaatc gacgatacgg acgcagagga ttttgatgat
    23881 tttgaaatca ttgtcacagg aaccatggct gatgtaattg agggcgaaga ctatactttt
    23941 tgggggcaaa ttgtccagca ctccaagtat ggagaacaac tgcaaatcag tcgttatgat
    24001 cgcgcaaaac caactagtaa gggcttggtc aagtactttt caagtagcca tttcaagggg
    24061 attggtctca agacagctca gaaaatcgtg gatacctatg gcgaaaatac cattgacgaa
    24121 attttgcaac acccagaaaa gttagaaggc atcgcaggac tctctgccaa aaatcgcgag
    24181 gctttcgtct ccactctccg tctcaactac ggaacggaga tggttttggc caaactagcc
    24241 aactacggca ttcccaacaa actagccttt cagattcaag acttttacaa ggaagaaacc
    24301 cttgatgtgg ttgaaaatta tccctaccag ctggttgagg atatcaaggg tttgggcttt
    24361 accattgctg accaactagc tgaggaacta ggcatcgaaa gtcaggctcc tgaacgcttc
    24421 cgcgccggtc tagttcacag tctttttcag gcctgtatgg aaacagggga cacctatgtt
    24481 gaagcacggg atttgctgga acaaaccctt actctccttg agtcttcccg tcccgtggaa
    24541 ctagacccca gccaagttgc ccaagagctc tcctacctga tcgaagaaga caaggttcag
    24601 cagattgata ccaagatctt tgacaacagc ctctttttcg ctgaggaagg catccgcagt
    24661 cacttggttc gtatccttga aaaaggaaaa cagaagagtc atgatttaaa aaccattcaa
    24721 aaacatatca ctactatcga gaaagagctt ggaattgagt atgataacat tcaaaaacag
    24781 gctatctgtg atgctatcca gaataaggtc tttatcctga caggtggacc tggtactggt
    24841 aagacaactg ttatcaatgg gatcatcgct gtttacgccc tgttagaggg acttgacctc
    24901 aggaagaaaa gcaacctgcc cattcttctt gctgctccaa ctggtcgagc tgctcgtcgc
    24961 atgaatgaat tgacaggttt gcctagcgcg accatacacc gccacttggg aatgacaggt
    25021 gacgatgata ccagtcatct ggaagattac ctagatgctg actttatcat cgtggatgaa
    25081 ttctctatgg tggatacttg gctggccaat caactcttct ccagtatctc ttctaacagt
    25141 aagatcctca tcgtaggtga cagtgatcag ctaccttctg tcagtcctgg acaggttcta
    25201 gcggatctgc ttcatattcc tttgattcct cagactcgct tggaaaaaat ttatcgacaa
    25261 agcgaagaat caaccatcgt caccctagct agtcagattc gacagggcat cttgccagct
    25321 gatttcaccc aaaaaaaagc tgaccgttcc tactttgaaa ttgctagtgg ccatattcct
    25381 gccaccattg aaaaaatctt aggtgccgcc ctcagaaatg gtattcctgc ccgtgatatc
    25441 caagttctgg ctcctatgta ccgagggacg gcagggattg atgctatcaa tcagctcatg
    25501 caagacctgc tcaatccccc acaaaaagat caactcagtt ttgaagctcc tcagtgccac
    25561 tatcgtaagg gagacaaggt cattcatttg gttaacgatg ctgaaatcaa tgtctttaat
    25621 ggggatttag gagctatcac agacctgatt cctggtaaat acaccgaatc gaaacaagac
    25681 gagattgtca ttgattttga tggtaatgaa gtctcttacc cccgtaacga atggtacaag
    25741 attcgcttgg cctatgccat gagtattcat aagtctcagg gaagtgagtt cccagttgtc
    25801 atcctaccga ttactagtgc tagccgacgt atgctggagc gaaatctcat ctacacagcc
    25861 attacacgtg ccaaaagcaa actcatctta ctaggcgaat tacaagcctt cgactatgct
    25921 acccaacaca tcggaactgc ccgaaaaacc tatctgattg aacgcttcag tgatctactg
    25981 gagaatgttg aagaaaagca acaagctgtc tctgaaacag tcacatcaag tgcctctgaa
    26041 caatcctaca tcctaaccga agaaaactgg gacagcattc cagccatgat tgggattaca
    26101 gacacagacc tcaaagagat ttttggaaaa tagtacatca gaaaacccac caattcaggt
    26161 gggttttctg tttattaaga ttattttact gttgctgtgc ttgtgatcaa ttcaacagct
    26221 tttttgatag tgatatcatg tttcaacatg tcagctgaaa gcaagttttg aacttgtgca
    26281 acttccatgt tgtagtctgc tgccaattgc tcaacttctt tttggatttc ttcttctgaa
    26341 gcatcaaatc cttcagcttt ggcaactgct tcgataacaa ggttagtctt agtacgtgac
    26401 tcagcttctg cttggtattg gttgtgaagg tcttcttgag tagttccagt gatttggaag
    26461 tacatgtcag ggttgatccc ttgacgttgc aagttcccaa ggaattcatt tactgaacgg
    26521 tgaacttctt catggatcat ttcttctgga agttctacga tttcagcgtt ttctacagct
    26581 gtatcaattg ctgcaccttc aactgcatct ttgtaagctt cttctttagc agcagccaat
    26641 tctttgcggt atttttcttt caagtcagca agtgtttcaa cttcttcatc aatgtctttt
    26701 gcaagttcat cgtcaagagc tggaacttct ttagctttta cttcgtggat agttgtcacg
    26761 aatttagctt ctttacctgc aaggtcttct gcttggtagt cttctgggaa tgttacgata
    26821 acatcaacgg tttcgccagc tgagtgacct accaattggt cttcgaaacc agggatgaat
    26881 tgacctgaac caagtccaag tgagaagttt tcaccttttc caccgtcaaa ttcaacaccg
    26941 tcgatagaac caacgaagtc gatcacaaca gtgtcgccgt tttcagcagc agcttccttg
    27001 ataaccaatt cagccaggtt gttgcgttcg cgttcgatac gctcttcaac atcagcgtca
    27061 gttacttctt tttctacatc aactgatact tcaaggtttt tgtagtcacc caattttact
    27121 tcaggttttg taacgacttc agcagcgata acccagtctt gacctttttc cattgaagtt
    27181 acgtcaattt ttggttgggc aaccacttca agaccagctt cttttacagc tgcttcataa
    27241 gcgtttggca aaagagcgtt cataacgtct tggtaaagtg attcttcacc aaatttttta
    27301 tcgaagatag gacgtggaag gtgaccttta cggaaacctg gaacattaag agatttcttc
    27361 actgacttga agacacggtc caattctggt ttgatttggt cttgagagat agtgaaagtc
    27421 aagacaccac ggtttgtttc tttgttttca aatgatacag acattctgtc atttctcctt
    27481 aaaatttttt aaatacagtc tattataaca ttttctataa ataaaatcta gactaatgca
    27541 gccaattata gacaacaagc ctttttccta actaaaacac tcataaagta tagacaattt
    27601 tagactactt tggatatctt ttggttagtc attttccaaa actaatttat ctaatttttc
    27661 agttatatag tagattgaaa ctagaatagt acacctctac ttctaaaaca tttttagaaa
    27721 tcgctttgac tgtcctgatc gatttgtcct gttcttgttt cattttacta tatagatcgc
    27781 attcgaaaga attcgttcca aaattgcttg gttcgttctt ctcttggacg actaaagaat
    27841 tgatgggcat tatttttttc aacaattttc ccaccgtcca taaaaataac atgatgggct
    27901 acatctctag caaaacctat ctcataaagc tgatcaaaaa ctcaatctga ggagaaaatc
    27961 caataaggct ttccataata aaggacatcc tatcaagatt tttgaacacc cgataggctg
    28021 cctttttccg attttgaaga ctttttgtcc agcttctttt accttataaa taacaacaaa
    28081 aatcaaagac aagctagaaa gattaccgca gataactcaa acagtgtttt gaggttgcag
    28141 ataagactga cggagtcagc tcaaagcact gctttgaggt tgcagataag actgacggag
    28201 tcagctcaaa gcactgcttt gaggttgtag atagaactga cgaagtcagt aacatatgcc
    28261 taagacaaag cgatgctaac ctgatttgaa gagattttcg aaaaatatta aatatactct
    28321 acaatttgct caatcaattc ttggtcgtct aaacctgtaa cttgtataac aacatctttg
    28381 actgattgtt tagccaacaa ttcacctaat cgaatacttt cttcatcatt tacatcgcga
    28441 tagtcaaagg catagccaac tgttttaagt aggtttttat atgacaaact gagttctttc
    28501 aattcacgta tcggccggat gaatcgttca ttatagccta attttcggat tggagtacga
    28561 gctacgcgac taacctcgtc cactatgaaa gggttttcaa accgttctat aatgactttg
    28621 tgataattct ccaattcttt tttatcaaag ttccatttgg caatcaagag actccgaatt
    28681 tcagctaata cagattcaat ccgagattta atattaggat tttgaagagc ttccaaaatt
    28741 gtcttggcac cataatgcgc accaatgtaa gctgaagttg catgtccaga attgactgaa
    28801 aaaagttttc gctcaataaa gggttctaaa tcttcttcat aatgcacatc ttttagacgt
    28861 aaatctggat ttttaagacg cttggtttcc acgacccatt cattaaaggg ctcgaccaca
    28921 acaaaaaggg aatcttcgtg actttgtgct ggaacaatcc tgtctactgc agcatttgga
    28981 aaacctatgt agttatcagc aaatgtcaaa ccttccggac ttaaatattt cttgacttct
    29041 tgataaagaa attgagaccc gccaatcata ttttcacagg ccataacatc caatacctgt
    29101 gtattccctg caactcggcg agcttcgatt cctttggcta gaagttcagc gataaaaggg
    29161 agtatattag gtccgattgc agtagtaata atatccgtct tttgaatcgc ttcaatgact
    29221 tgctcaggat gttctttgct attaattcca gccacattag ttacttctat acgagactgt
    29281 cctttctgtg caatttcaat ttcatacttg cccttttcat ttagagcatg aattatctga
    29341 ttattgacat ccacaaaatc aatatggaaa ccatttttaa atagaatttc acctataaaa
    29401 ccacgaccga tattaccggc accaaaatga acagaatgtt tcattcaatt tctccttttc
    29461 ctatttgagg gacatatata ctagatggca aatgacgaaa ttcaattatt caacagcatc
    29521 aaataagcgc aatacttcct cttttgactg agcatcggct aatttaagaa cattatctac
    29581 atctgcacag aagatagaaa ttttctgaat aatttctaag tgttcattac caataccagc
    29641 aataccaaaa agaaccgttg ccacttgagg gttagataca ttcccaaaat caacccctct
    29701 agggacttga acgactgtaa taccagactt gaggacatcc ttttttgctg catctgttcc
    29761 atgcggtatt gcgataaagt tacccatgta aacagatagc tctttatctc gctcaatcat
    29821 ggcttcaata tagtcttcat taacatatcc accctcataa agaacttgcc cacaataaca
    29881 aatagcttcc tccttgttag aaaattgttt attaagctta atcaaatgtt tttcaagttt
    29941 catattagtt ctccaatttc ttaatttttt cgttaaaaat agtatttagc atctgataaa
    30001 taatggattg atttcccgtc ttgtaaatct ctgtataaag atgattttca ataatcgact
    30061 gactaatagc tgtcattaaa tctctcgctt cctcgctagc ttctttagac attagcatta
    30121 gcagacacct tttgacctct tcttcctctc gtttcattga caatgcggag ataggtagtt
    30181 tgagttcaaa catagtaaaa ctatttgttg ttactttgct agactgacta tgtaaaagca
    30241 ccagaccagt attaggaata gccataggac tattctggaa gtgagataac aattttcttg
    30301 ttaggtaatc tctgtcactc acattcttct gatattggat gatttgttct accgtgtgct
    30361 caaatgattg attattctcc aattgaacca actcgaattg actcaaaagc tgactactag
    30421 atattaaata atcttgcaag tcataacttt tctctggagc gatagcatca cgcgcgacaa
    30481 ttgtacgatg tgcctgtaca tactgaagtc gttcctgtaa ttcaagcaat tctttgacgg
    30541 ttggaaaact agaaattaca tcgatttcct gattagtcag cggcttggta gataaaatat
    30601 aatcatactg actgagatca atactgtggt aatctactag agactgaata tctatcaact
    30661 ctacaaaagg agcaacactc ttaattttac tgactaataa atctctagtg acccgacgtt
    30721 catccgttaa aagcaaaaca cggactggat aaatctctgg actacggcct aagctagaga
    30781 taaaatgtag ggcaatcatg ccatactcat actctgtatg aagatatttc ggaaaaatat
    30841 cattcactaa aagactgatg actgtataaa gaaatttatt cctttcaact aaaatctgga
    30901 cagattctgg caaattttca ccctgaaaaa ggatagggac gcctaaactg agccgaatat
    30961 gatggaaaag aaaattgaat aaaaccttgt ccttaaaaaa gtcaatcttg gtatacatgg
    31021 aaaccgtatc aatcagattt gaaatattgt agaaaaattc accatcaaat ttctccgtaa
    31081 agagcggatt gtcctgacgt ttaatgatta attcatccaa gatgctcgca aaatagataa
    31141 tttcctgaat actataaaat tgtgcagtct gcttagagta agcttgaaaa attttttgag
    31201 aaatttccaa agcctgctta ctagtattag gtgaattttc aatgttttgc tcctgaccta
    31261 taagagataa caagatcgca aaaaacatct tcatcctagc atccatatct ggaaaacctg
    31321 acagttgctt attaacaatc tgactggcca gcctagttct atctgcttct aaaatatcaa
    31381 agctcccaaa attaccggtt gaaaaatctg ctactgagat acagtttgtc agtaaaatag
    31441 ccaaaaatct tctcttacca actgaatcac cagaaatccg ataacctttt tgtcgttcaa
    31501 ttttcagatc aaagtctaaa agacgcttat caatatctga aatatcctga ataatagtta
    31561 cattactgac tttcgtgcac tcttgcaatg cttcattggt gacaaaacca ctctcagtca
    31621 gaaggcgata ggtaatcaac tcttgtcttt cttggggact atactcaact aacacttcaa
    31681 gttctgtcgg caaatcatcc aactctccag tcaaaatata gtatttccca tgcttcgtta
    31741 tttcgattcc atattgctcc atgctatctg tcaaatctga taaagttcga taaattgttc
    31801 tagatgaaac ctgtaagatt tcagtcatat cttgcattga aagcttccct acatgtagga
    31861 aagccttcaa taattgttct tctcgtttgg ttaatagcat tcgtaattta gaaacctcta
    31921 gttctatttg tacattctag cagccatttt gtcatattct ggtgttgtta ctaaactatc
    31981 cacaataaga aattgagaat tcggtgctgc ttgctgcact tctgcctgta aagaaatagt
    32041 tgttacaatc attatgtttt tagaattaaa ttcacctaat tctgaaattt tggcagtaga
    32101 aactggaata cgaatattct tgtttctaaa gatagcccga atcgtttcac agcccatagt
    32161 tgcagttccc tgtgctttac cataagcaac tactacagca tcaatatggt taaggtcaat
    32221 ttcctgacta tctactggtg ctgaagttgg tatatctcct tcaatttctg ctattggaaa
    32281 agctcctgtt aatgaagcta caatttcatc ataacgaggg gacgctaaga aattatcaac
    32341 agaaacatga atagcacttg gactcttgtc tttagctctt ggtgtcagtt cttcctgagt
    32401 aacaattaat gtttttggtg tatcaagcaa atttgagatt gcctgattag atactggaat
    32461 ctctagacct gcttttttaa ccttatctcg aagaatacta gctcccatag cagagcttcc
    32521 cataccagca tcgcaggcga aaatgatttt ttccactgag tctgtcgaaa taactgcatc
    32581 aacagaagtt gatactaact gacctttaga ctgagcttta gctgcttggg tagcctcttg
    32641 agcagcttcg agcgaatcct cagttgactt gtctgcatga agaataaggg ctgctacaaa
    32701 gaaagaaaca actgctgcca ctaaaacacc tagaagaaca tttagatggg gccaaacacc
    32761 ttttggcgcc gtagccataa tcgcaataat agaacctggt gaagctggag atttaagacc
    32821 agcgtctaag agttgaaaag taaaagttcc agagatacct cctgccatag cagctaaaaa
    32881 tagagtaggc ttcatcataa cataaggaaa gtaaatttca tgaatccctc cgaagaaatg
    32941 aataaccatt gccccccaag aagaagattt agcagaacct ttaccgaata cagcataagc
    33001 taatagaatt cccaggcctg gtccaggatt agcttccaat aggaagagaa ttgacttacc
    33061 agcttgagct acctgttcta ctcccagagg agtaaaaatg ccatgattga gggcattatt
    33121 gaggaaaagg actttagccg gttcgatgat aatattagct ataggaagga ggcgagcatt
    33181 gacaatagcc tcaacaccat tcccaacagc tccagtaaga gtcgatacga ctggaccgat
    33241 tgcgtagaaa gccaaaagca ataatgcaaa accaacgaga ccagctgaga agttattaac
    33301 taacatttca aatccgggac gaattttttc ctggaacttc tcatcaaatt tcttgatagt
    33361 ccatcctccc agtgggccca ttaccatagc tccgataaac ataggaacac tagaacctgt
    33421 gattgcacca acagtagcaa tagctcctac aacggcacca cgttggccat ggatcatata
    33481 tccacctgtg taaccaatca ggattggcaa taaatacgtt aacataggac caacaacagt
    33541 agctaactgt tcatttggca gatagccatc agcgataaag agggcagtca atactcccca
    33601 agcaataaat gctccaatat tgggcataac catatttgaa agcgatgtcc ctagtttttg
    33661 aaccctgact ttcaatgata ctttttcttc cattagatca tcctcctttt cttaaaacta
    33721 gtatatacaa agttaaaaac ctttaaaact aaagttttgt cacaaatttt gtgtcaaaac
    33781 attacttttg acgaggctgg gcaaaaaggc ttaaaaatga taaaacgcat catatccggt
    33841 gtac
//
LOCUS       DAVKMU010000020        33186 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKMU010000020 DAVKMU010000000
VERSION     DAVKMU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.020"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..402)
                     /locus_tag="VW343_001082"
                     /pseudo
     CDS             complement(1..402)
                     /locus_tag="VW343_001082"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
     gene            complement(435..773)
                     /locus_tag="VW343_001083"
     CDS             complement(435..773)
                     /locus_tag="VW343_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9755647.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADFISKSQLSRFERGENEISL
                     STFFELLSNINVSIENFCNHLEYYKRSERDDFLVNLSPNFYSLNIKGLEVIKNKQQKL
                     FEKSGKKLIK"
     gene            complement(786..1559)
                     /locus_tag="VW343_001084"
     CDS             complement(786..1559)
                     /locus_tag="VW343_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9755648.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            complement(2005..2760)
                     /locus_tag="VW343_001085"
                     /pseudo
     CDS             complement(2005..2760)
                     /locus_tag="VW343_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(2898..4520)
                     /gene="groL"
                     /locus_tag="VW343_001086"
     CDS             complement(2898..4520)
                     /gene="groL"
                     /locus_tag="VW343_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9755649.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(4536..4820)
                     /gene="groES"
                     /locus_tag="VW343_001087"
     CDS             complement(4536..4820)
                     /gene="groES"
                     /locus_tag="VW343_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9755650.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(5090..5485)
                     /locus_tag="VW343_001088"
     CDS             complement(5090..5485)
                     /locus_tag="VW343_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9755651.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(5563..6324)
                     /locus_tag="VW343_001089"
     CDS             complement(5563..6324)
                     /locus_tag="VW343_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9755652.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(6357..6983)
                     /locus_tag="VW343_001090"
     CDS             complement(6357..6983)
                     /locus_tag="VW343_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9755653.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(6999..7316)
                     /locus_tag="VW343_001091"
     CDS             complement(6999..7316)
                     /locus_tag="VW343_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9755654.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(7313..7612)
                     /locus_tag="VW343_001092"
     CDS             complement(7313..7612)
                     /locus_tag="VW343_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9755655.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(7862..8050)
                     /locus_tag="VW343_001093"
     CDS             complement(7862..8050)
                     /locus_tag="VW343_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEU9755656.1"
                     /translation="MKASTWVYDKGEWYYVSSSGAMLANDWVKDNGKWYYLASSGKML
                     RNTYTPDGYYVGNSGAWQ"
     gene            complement(8301..8975)
                     /locus_tag="VW343_001094"
     CDS             complement(8301..8975)
                     /locus_tag="VW343_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755657.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            complement(8981..9427)
                     /locus_tag="VW343_001095"
     CDS             complement(8981..9427)
                     /locus_tag="VW343_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9755658.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(9541..10044)
                     /locus_tag="VW343_001096"
     CDS             complement(9541..10044)
                     /locus_tag="VW343_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9755659.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(10041..10403)
                     /locus_tag="VW343_001097"
     CDS             complement(10041..10403)
                     /locus_tag="VW343_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEU9755660.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFLQAPYYVPERIAITGKICGVHIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(10469..12328)
                     /locus_tag="VW343_001098"
                     /pseudo
     CDS             complement(10469..12328)
                     /locus_tag="VW343_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(12367..12816)
                     /locus_tag="VW343_001099"
     CDS             complement(12367..12816)
                     /locus_tag="VW343_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9755661.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIDQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(12940..13656)
                     /locus_tag="VW343_001100"
     CDS             complement(12940..13656)
                     /locus_tag="VW343_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9755662.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(14537..15952)
                     /gene="ply"
                     /locus_tag="VW343_001101"
     CDS             complement(14537..15952)
                     /gene="ply"
                     /locus_tag="VW343_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9755663.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(15963..16295)
                     /locus_tag="VW343_001102"
     CDS             complement(15963..16295)
                     /locus_tag="VW343_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755664.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(16366..16974)
                     /locus_tag="VW343_001103"
     CDS             complement(16366..16974)
                     /locus_tag="VW343_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755665.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(16987..17445)
                     /locus_tag="VW343_001104"
     CDS             complement(16987..17445)
                     /locus_tag="VW343_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9755666.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            17570..17969
                     /locus_tag="VW343_001105"
                     /pseudo
     CDS             17570..17969
                     /locus_tag="VW343_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(18734..18994)
                     /locus_tag="VW343_001106"
     CDS             complement(18734..18994)
                     /locus_tag="VW343_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755667.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
     gene            complement(19053..19604)
                     /locus_tag="VW343_001107"
     CDS             complement(19053..19604)
                     /locus_tag="VW343_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755668.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(19594..19791)
                     /locus_tag="VW343_001108"
     CDS             complement(19594..19791)
                     /locus_tag="VW343_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755669.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(19795..20589)
                     /locus_tag="VW343_001109"
     CDS             complement(19795..20589)
                     /locus_tag="VW343_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755670.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(20997..21422)
                     /locus_tag="VW343_001110"
     CDS             complement(20997..21422)
                     /locus_tag="VW343_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755671.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDNNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(21551..21934)
                     /locus_tag="VW343_001111"
     CDS             complement(21551..21934)
                     /locus_tag="VW343_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755672.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQLASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKEMVLG"
     gene            complement(21956..22162)
                     /locus_tag="VW343_001112"
     CDS             complement(21956..22162)
                     /locus_tag="VW343_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755673.1"
                     /translation="MDLSNKASNLRKKLGADGEAPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(22149..22322)
                     /locus_tag="VW343_001113"
     CDS             complement(22149..22322)
                     /locus_tag="VW343_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9755674.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLYLSLNQSMIAK
                     MEKGERNITNGFK"
     gene            complement(22694..23650)
                     /gene="lytA"
                     /locus_tag="VW343_001114"
     CDS             complement(22694..23650)
                     /gene="lytA"
                     /locus_tag="VW343_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9755675.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADRPEFTVEPDGLITVK"
     gene            complement(24026..25396)
                     /locus_tag="VW343_001115"
     CDS             complement(24026..25396)
                     /locus_tag="VW343_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9755676.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(25710..26876)
                     /gene="recA"
                     /locus_tag="VW343_001116"
     CDS             complement(25710..26876)
                     /gene="recA"
                     /locus_tag="VW343_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9755677.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(26931..28187)
                     /locus_tag="VW343_001117"
     CDS             complement(26931..28187)
                     /locus_tag="VW343_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9755678.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(28272..29288)
                     /locus_tag="VW343_001118"
     CDS             complement(28272..29288)
                     /locus_tag="VW343_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9755679.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(29296..29814)
                     /locus_tag="VW343_001119"
     CDS             complement(29296..29814)
                     /locus_tag="VW343_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755680.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(29804..30247)
                     /gene="tsaE"
                     /locus_tag="VW343_001120"
     CDS             complement(29804..30247)
                     /gene="tsaE"
                     /locus_tag="VW343_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9755681.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(30333..30953)
                     /locus_tag="VW343_001121"
     CDS             complement(30333..30953)
                     /locus_tag="VW343_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755682.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(31336..32199)
                     /locus_tag="VW343_001122"
     CDS             complement(31336..32199)
                     /locus_tag="VW343_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9755683.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            32664..32888
                     /gene="pneA1"
                     /locus_tag="VW343_001123"
     CDS             32664..32888
                     /gene="pneA1"
                     /locus_tag="VW343_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEU9755684.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            32895..33083
                     /gene="pneA2"
                     /locus_tag="VW343_001124"
     CDS             32895..33083
                     /gene="pneA2"
                     /locus_tag="VW343_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEU9755685.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT        10072 a         7184 c         5598 g        10332 t
ORIGIN      
        1 gtccatttgt ttaattccag attctttaga ttctgtcagg tatattttat atccttctac
       61 aaatttgaat gtagattttt caaaaacttt tgtgtcatct tttaataatt tttgtaattt
      121 catttcaaag tagtctgcat atattagatg tgaattttca aggcaacaga accaaaaatt
      181 taaacaagct gacacaacta aatttctatt cttgccgatt ttttcgtaat aatgtgtacg
      241 ctctagtatt tcttttccga ccatgtaaat ataatctatt tcaaaaagat gatataaatt
      301 accaatcagc gttatctcat aggactccca tcgttccttc tggaataaat agtcatatac
      361 taaatttaga tcatctctac taactacatg attacaatct agctgatttc ataatccctg
      421 aaccataatt gtatttattt tatgagtttt ttcccactct tctcaaaaag tttttgttgc
      481 ttatttttta ttacctccaa tcctttaata ttcaaactat aaaaatttgg agataaatta
      541 accaaaaaat catcacgctc cgatctttta taatattcta agtgattaca aaaattctct
      601 atcgaaacat tgatatttga taataattca aaaaatgttg aaagagaaat ttcattttct
      661 cccctttcaa atctagaaag ttgagatttt gatataaaat cagctgcagc atactctaaa
      721 gaatatccgt ttaattttct aaaatatcga aaagcttctc cgtaattttt cacactttca
      781 ttcccttaac tttcttttcg agcgataaac ataccatatt ctttaggaat gatcaacata
      841 ccattattaa actcactttc caatctttta cgtattattt cctcttctag tgaacctatt
      901 tctgaaattc ctttaaacga ttggatgtat ttaactaggt catctatact agttacctgt
      961 aactcatcat catagagtaa tgtatcgaca gagttaaaat acctactcag gtacctttta
     1021 ccattttgta aagtaaacgt tctattttct aaatcttgat taacctcatc tttaaataaa
     1081 cttgccaaat aatttacaac tccattttca ccaaatgtag cacagtaaaa aatgcctccg
     1141 gtttttaaga ccctattcac ctcagagaga gctttaggaa tatcattcac atgatgtaga
     1201 agcatattag caataacaat atcaaaagtt tcattctcaa aagaaatctt ttgaatatcc
     1261 attatctcat aattgacatt gtttctattt ccaatcactg acttcgttga ttttaccata
     1321 tctttagaaa aatctgtaac aatcaactgc ttcattttat ctatagaatc actattactt
     1381 ttccacaatt ctcctgtacc acatcccaat tctagaacct ttacttcatc agtgatttgg
     1441 tagttagaaa atatccaatt attaaatccc aatttatttt ttgaatactt cttatgcaat
     1501 tctactcgaa tatcaagatt atcatgactt ttatattgtt caattacttt atcaatcatt
     1561 ttaaatgacc tccactctat aatttggcat tatatcacaa gaataaagat agtgttaaat
     1621 aagttatata aatgcaaaaa aggaatattc cgttatagca gagttgtaac acatttctat
     1681 aaagaaattt attcctacga ctcagtttgg catgcttaac ttcctaactc aaaaacaaag
     1741 catcgattaa tttatccgtt cttcttttga aacagatttg aactgataat tgctcgtttt
     1801 aatttaaaaa gctcttttga gcaaaacttc atttttttaa gagtccaaca atttctgtct
     1861 atcgcttttc tactagcaaa aaacctacct tgaggcaagt tttcatttat atatctaatt
     1921 tcgtaacagt atagtaaaat gaaataagga cagtcaaatc gatttctaac aatgttttag
     1981 aagtaaaggt gtactattct agtttcaatc tactataaat ttgatatgag aatagtcttt
     2041 ctcaacatcc ttatccaaca agcatgctgt ggcgtccttc ttaacttaaa ccaggtcaat
     2101 attctgggct tgggctgcat agacgcagcc acaataacat tgacgataga tatcatactc
     2161 ctcacacatc tctactgaac gtttgtagcc ttgatttttc ttgaaatcgt tgggaagata
     2221 gtggggcgtg taaatttttt gcacatcgat tccgatgcta ttgatagttt gagaattctt
     2281 atgaggactg atggtcaagg ctgaaccaaa gtagtcaaag cccaagtcca tagccacttg
     2341 cgctgtttta tccagtcggt agtcaaaaca aaccttgcaa cggtcgccac cttcgggctc
     2401 ctcctctagc cccctaacta gttttcggta ttaattgggt tcgtagggag cttctaggta
     2461 ctgaaccgta tttcctgtcc gctcattaaa atcactaaca aatttcttgg tgacatagac
     2521 ccgcttatgg tattctgcct tgggatggat attagaattg gcaaaataga tggtcacatc
     2581 tgcatacttg gtcaaatatt ctagtgtata ggtactacaa ggggcacagc aaacatgcac
     2641 gagaatggtt ggccgctact catttttttc ccatgcttgt accattttct gcatgacacg
     2701 gtcataatta atcttctgat tgggggtcat cttgcacaga atttcttcta catcgatcat
     2761 gttcttctcc tttttctagt cttattttat catataagtt aggagagctc aaatcaattt
     2821 agaagtgaat attataaaaa gaagggttag tcattccctc cttttgtgtt ttttataagt
     2881 tgttttctat agaaagctta catcatcccg cccatcatac ttggatccat tgctggagct
     2941 ggggctactg gttctggttt attggctacg actgcttctg ttgtcaaaat caagctggct
     3001 acagatgctg cattttgtag ggctgaacga ctcactttaa ctggatcaat gataccttga
     3061 tcaatcatgt taacccactc gccagttgct gcgttgaatc ctataccaag ctcagcattt
     3121 ttcaaacgat cgataacgat agatccttca aatcctgcat tgtgagcaat ttgacgaaca
     3181 ggttcttcca aagcacggag aacaatatta cgtcctgttg cttcatctcc tgtcaattcc
     3241 aaggtagcaa cagctggaat cacattggca agagctgttc caccacctgc aacaatacct
     3301 tcttcaacag ctgcacgagt agcgttgagg gcatcttcaa tgcggagttt catttctttc
     3361 aactcagttt cagttgcggc tccgacctta ataaccgcta caccacctga caatttggcc
     3421 aagcgttctt gcaatttttc acggtcaaat tcagaagttg tagtttcgat ttgagacttg
     3481 ataaccgcaa cacggtgaga aatcgcttca ggatttcctg caccttctac aataaccgtg
     3541 ctatctttgt ccacggtcac tctcgctgct tgaccaagag cttcaattgt cgcatctttc
     3601 aactcaagac caaggtcttc tgtgataact gttccgcctg ttaagatggc gatatcttca
     3661 agcatggctt tgcgacggtc accaaaacca ggtgccttga ctgctactac gttgaaggtt
     3721 ccacgaatct tgttcaaaac aagagttgga agagcctcgc catccacatc atccgcaata
     3781 atcaagagtg gacgattgct ttggagaatg ctttccaaaa gtggcaagat ttcttggata
     3841 ttggaaattt tcttgtctgt aatcaaaatg tacggatttt caaggtcagc caccattttt
     3901 tcgctatctg tcaccatgta ctgtgaaagg taaccacggt caaactgcat tccttctacg
     3961 acttcaagct ctgtttccat accacgtgac tcttcgatgg tgatgacacc gtctttgcca
     4021 actttttcca ttgcttcaga gatgtactca ccaacttttt cagaacgaga agatacggct
     4081 gcaacttgag cgatagcttc tttattggca acagggatgg cgttgttttt caaagcttca
     4141 actgctgcgg caactgctgt ttcaatccca cgacgaatac cgattggatt tgcacctgct
     4201 gtgacgtttt tgattccttc acggacgatt gcttgggtca aaacagttgc agttgtagtt
     4261 ccatcacctg cgatatcatt ggtttttgaa gctacttctg ataccaattt ggcacccata
     4321 ttttcaaaat ggtcttctaa ttcaatttct ttggcaatag tcacaccgtc attggtaatc
     4381 aatggtgagc caaatgattt ttcaagaacg acattgcgac cttttggtcc caaagttact
     4441 ttaacagtat ctgcaaggat atcgacacca cggaccatgg ctgaacgggc atctgatgaa
     4501 aatttaattt cttttgacat acttactttc tccttctatt cttcaatgat tgccaagatg
     4561 ttagcttcgc ctacgatgat gtacttttca tcgccatctt tgacatcaag acctgcgtgg
     4621 gcttcaacta agacacggtc tccagtttta acacttggag caaccaagtc accgttcaag
     4681 gtacgaacac cttgtccagt agccacaact tgggctgttt tggttttttc ttgggctgaa
     4741 cctgcgagaa caaagcctcc aacagtttgt tctttttctt ctacttttaa gaccacacgg
     4801 tcccctaatg gtttcaacat ctgatttcct ccataatgag atagatatta ttagcactct
     4861 ttatacctga gtgctaattc atagttctat tgtatcactt agtcagaaat aatcaagaaa
     4921 aaagtctgac tttctcaaga taaaaagcct gagaccaact cagacttttt aatgtttata
     4981 ctcaatgaaa atcaaagatc aaactaggaa gctagccgca ggctgctcaa agcattgctt
     5041 tgatgttgtg gataaagcta acgtggtttg aagagatttt cgaagagtat taaaatggca
     5101 attcttcctc ttccaagact aaatctgcca aatcttggcc tgcattattt tcacgcatgg
     5161 cacgttgagc acgactttcc aagagttgga atcctgttac aagtacttcg gtcacgtagt
     5221 tcatttggcc atttttctca aagcgacggg tacgcaattc tccatcaacg gaaatgagac
     5281 tacctttggt tgcgtagctt gccaaagttt ctgctagtct gccccatagg accatattga
     5341 caaaatcagc ttcacgttca ccgttttggt ctttgtaacg acggttgaca gcgatagttg
     5401 ctcgcgctac cgacttgtca ttgttggttt tgtgcaattc tggtgtagac gttaaacgcc
     5461 caatcatgat aactttatta tacatatttt cttcctccta cttatctatt cgtaggaaat
     5521 caaaaaaagt tacagaaatt tgtaactttt cgaggaaatt tcttattttt tatgaatcat
     5581 gaaacctgtc gcctgttgat tggccataat ggtcatatct gtaatctgaa cacggcgagg
     5641 ctgactggtc acatagacta ctgtgtctgc aatatcctga gcttgcaagg cttctattcc
     5701 ttggtaaacg gacgcagctc gctctttatc accatgaaaa cgaacagttg agaaatctgt
     5761 ttcgacaatc ccaggctgaa tggttgtcac cttgatatcc gttgcgatgg tatcaattcg
     5821 cagtccatcc gaaaaggtct taaccgcagc cttggtagct gagtaaacag cggcaccagc
     5881 ataggcgtag attcctgcgg ttgaccccat attgataata tgaccttgat tgacttttat
     5941 cattgctggc aagaaacagc gagtaaccgc catcagacct ttaacattgg tatccaacat
     6001 ggtcaacata tccaactcct catagtcttg atagggagcc aagtcaagag ccagtccggc
     6061 gttgttgacc aagatatcaa tcctccctat cgtttctaga atatcagagc aaacagtctt
     6121 caccatgacc atatccgtca catctagtgg aaaagtccag acggtttgat ttggaaagct
     6181 tactgcaaac tccgacttga ggatttctaa tctgtctatc cgtcgtcctg ttagaacgac
     6241 atcctcaccc tgctccagat aagcacgcgc aatcgcttca ccgattcctg aggttgctcc
     6301 tgtaatcact acattttttg ccatcttatt tccttctatc agatgctgac aagttcctag
     6361 gcagtccagt gtttcgctgg gtcgaacggt gttccgacaa cttggtcttc tgataattca
     6421 agcaccccac gtttttgtgg agcatttggc agatgcaatt cacgaggact gcacatcata
     6481 ccaaaactct tttcaccacg aagttcacct gggaaaatga gattcccttt tggcatcata
     6541 gctccaggaa gcgcgacaat ggttttcaac cccacacgcg cattgggagc tcctgcaacg
     6601 atttgtacag tcttatcact tgcgactgca acttggcaga tgttgaggtg gtcactatct
     6661 ggatgggcta ccatctcaac aatttcaccg acaacaaact taggttcctt atcattaaca
     6721 atttcttctg taaaaccttc cgcctgcaac tcttggttca aacgagcgac ttgctcatct
     6781 gtcaaaaaga cttgaccgcg ctctgcaatt tcaaataaac ttgaaacttc gaaaatattc
     6841 caagccactg tttccccatt atctttgaga aaaacacggg ctaccttgcc tttgcgctcc
     6901 acatccagtt tggcatctcc gctatttttc acgatgacca taaggacatc accgacatgt
     6961 tctttattat atgtaaaaat cattgtttcc tttttctcct atttcagtcc tgctaaaaag
     7021 tcattgattt gttgcttgct tttacggtcg cgattgacaa aacgaccgat ttccttgtcc
     7081 ttttctagaa caacaaggct agggattcca tatacatccc agagtttggc caaatctata
     7141 tactggtctc tatccactcg aataaaggta aactctggat tagtctcctc aatttctggt
     7201 aaggcaggat agatataacg gcaatcgcca caccagtccg ccacaaaaag gaagaccttc
     7261 ttaccgtctt tttctactaa agatgccaat tcttctaaac ttgctggttg tatcataaga
     7321 cttcctcctc atagactagg tcttcatttt catagacaaa agtataatga cggccatcct
     7381 caaaaatgac gccaccaagc aagctctcta gactgctttc atagacttga acatagaggg
     7441 tcgcaatttc ccccatgtct gaaaaatggt ctcgtacaat cgctgtcaac tcttcctgag
     7501 tcttcatgag cttatggtca tctgcaactt ttttcgtagc aagggcaagg ctcccaatac
     7561 caagcagagc cagacctgcc atccacattt ttttagcttt cataccattc attttaacac
     7621 aaaaaaggct ttaggacaaa tgaggaaaca gcaaaaaagc aagtaaaaag cctcttcctt
     7681 taaggaaaag gacttcttat actcaatgaa aatcaaagac caaactagga agctagccgc
     7741 aggctgctca aagcactgct ttgaggttgt agatagaact gacgaagtca gctcaaaaca
     7801 ctgttttgag gttgtggatg aagctgacgt ggtttgaaga gattttcgaa gagtattatt
     7861 cttattgcca ggcgcctgag ttaccaacgt agtaaccatc aggtgtgtag gtattgcgaa
     7921 gcatcttacc tgatgaagct agataatacc acttgccatt atctttgacc caatcattcg
     7981 ctagcatggc tccagaagaa cttacataat accattctcc cttgtcataa acccaagtgc
     8041 ttgctttcat ggctcctgag caattaaagg ttcaaaaaac tgtccaatat cattcatttt
     8101 tttaaagcat tttacactac atcatccacg ctgtaggaca tagatacatc aaacgtaaga
     8161 gccaaaatat cgaaaaatat acaactttta aaggcaacat ttcatttttt aaagtctaac
     8221 aaccatagaa ctgaaatcag agcaaggcat caacttccta ctcttagtac gtctattttg
     8281 atggttgaag tgaagatttt ttatttaacg tagacaagct ccaacttccc aaaagccaca
     8341 tctccacgga taatcaaggt tttgctggtt ggggctacag gagcgtcagc cttagctgca
     8401 ccaaaggagg tttctacttt caaatctaca cgccagtgtt gtggaacata gacggttgca
     8461 ttcccgaagg ccacttcaat atttaaagtt gcaaaatcac ctagcatctc tgcattatca
     8521 tagtagattt tagcatcccc aaaagcgact tccacttggt catctacgag atcttgatct
     8581 tgcttataga aggtcccgct accaaaagcg acttccttat ccgtgacgac tgtttttttc
     8641 ccattgtacc accatttttt ttcattccag aacttacttg aatgcgtcag ataaccaaca
     8701 ccaagtacca ccaagatgct agcccaaata agagaatgat tggtaactgg taacaagtca
     8761 taagcgtaat ttgcaatgat gagcgccagt aaacctgtaa aaactgccga agtgagatga
     8821 cgtctaagga tggactcaat ggacttataa gcaaaaaaaa caatacctag taaaggccat
     8881 attttaccat ccaaagaagg aattccaaaa ttcccttgca gcaagatcca agctgctaaa
     8941 accaataaaa caataccaaa tgctttcttt ttcatgtttt ttacctcatg tttcttagat
     9001 tttcttttag gagggattgg taatgccgtg agacatgaac ctccttgtgc gtctgataaa
     9061 aggaaatggt gcccgttcct gaaaaggact tgtccactga gtaaatctga cggatgtttg
     9121 cgatcgttga cttggaaact cgattaaaat agcgaggcaa gatagactcc aactcataga
     9181 gcttgaggcg aacctcataa gcttccttct ggttatgagc gtagatcttg ctcccttctg
     9241 tttcaaagaa gagaatttct gacaggtcta gataatattc acctgtcccc ttgtaaaaag
     9301 tcaacctcgg agcctttgac tcttgcaaga gccgttgcaa gtctgcaatt tcatctgtca
     9361 aagtgggggt cttgatgaca atttcagtct cctctaaatt gccgtcaatc tcaattcgta
     9421 acttcatcac atctcctttc tgactctact atatcaaagc taagttagga atacaagggc
     9481 aaaaaagcaa gtggtcaatt tgaagcagta agtggttgta ggactagctt aacttacatg
     9541 tcagaatagg aagaaaagga atccccacac cagaccacaa gcatagccaa ccaagacatc
     9601 cttgggataa tgaacacccc ctaaaaccct caccaacccc agcaaagctg aaaagatcaa
     9661 gcatactaag ccaataggta gactagcatg cagacaggcc atggagatga tagttgcaga
     9721 aaagacatgg cgactgggca tcgaccgtcc cgaactatcc ttatcaagaa ggggacagat
     9781 atcccaagtt tcataaggtc tcgggtgatt gattttctta cgaaacaggg acaaggccac
     9841 aaaacctgat gcaggaataa gcacataaac cccaacttgc tgaccaagtc ctagttgcag
     9901 gtaagtggtg actagcaaga tcagatagac caggggcatg gcgactgtca tcaaacgatt
     9961 gaaactgcgt aataggaata aaagaattgg acgactagtt agtctggagc tgatatttcg
    10021 ataccattct tgataatttt tcatttatgc ttctcatact tttctagaac ttgttgagat
    10081 gttttcccct tggcaagcca atccactagc ttgtccaaac gacgtatttc ctgcatcaga
    10141 ggatcatcaa tttcctcaat atggacaccg caaatcttcc ctgtaattgc aattcgttca
    10201 gggacataat atggtgcttg tagaaaaaaa tctccgtaag taacatccct atccagacaa
    10261 gcaagaacgt cactcacttc ataccctgtc aaccaactgg tcacttggtg aactgactct
    10321 gcctttccac cctttcgctc aacttttgca atcaaggcta agtagacagc agcaaatttc
    10381 atattgtata atttctgact catacttctc tctttctatg tgtactagta taaataagaa
    10441 aaagaaggcc gtcaagcctt tttttgattt attcttctgc ttcatcttct gtaaattgac
    10501 tattgtacaa gtcagcgtag aagccacctt gcgccatcag ttcctcatag ttgccttgct
    10561 cgatgatatt tccatctttc atgaccaaga tcaagtctgc atttcggatg attgacaagc
    10621 ggtgggcaat gacaaaggat gtgcgtcctt ccatcaaacg gtccatggct ttttggatca
    10681 attcctctgt ccgtgtgtca acagaagaag tcgcctcatc caaaatcaaa agcgatgcat
    10741 ccttaagaag ggcacaagca atagtcaata gttgtttttg tcctacagac aaggtcacgg
    10801 tgtcatccaa gatggtatca tagccatctg gcaaggtcat aataaagtgg tgaattccca
    10861 cagccttact agcttccatc attcgttcat cactaatccc tatttgatta tagatgagat
    10921 tgtctcgaat agttccttca aagagccagg tatcctgcaa gaccattgaa aaggcatcat
    10981 gcacttctga acgcgtcata gccttggtat ccacaccatc aatgcgaata cttctcttat
    11041 caatctcata gaatttcatc aaaagattga caatggttgt cttaccagcc ccagtcggcc
    11101 caacaatggc aaccctttga ccagcatgag ctgtcacaga gaagtcatag tcttgaacat
    11161 tgacaccgtc caccagaatt tctcctgctg acacgtcgta gaaacgtgga atcagattga
    11221 ccagagttga tttaccagaa cctgttgacc caataaaggc cactgtttga ccagtttctg
    11281 ctttaaagct aacatgttca ataactgcct ccgaatttgc cgcatagcgg aaggtcacat
    11341 ccttaaactc gacctgacct ttgaagtttt catcagtcag ctgcacttga acagggtttt
    11401 ggatagaaga atgcaaatct aaaacttgat taatccgctt agcagagacc atagttcggg
    11461 gaagaacgat gaagagtgct cccatgagaa ggaagcccat gacaacctac atggcataag
    11521 acatgaaaat aatcatgtca ctaaagagag gcagacgcgc tatcggagca gcgtcgttaa
    11581 tcacataggc cccaatccag taaatcgcta cactcaaacc acttgaaatc cccatcatga
    11641 taggattcaa aatagccata agacggttga caaacaaatt caaacgggtc aattcatcat
    11701 ttactgctgc aaatttttca ttttgataat cctctgcatt gtaggcacga acgacacgaa
    11761 tacctgttaa actctcacga gtgatactgt tcagtttatc tgtcaacccc tgaatcaagg
    11821 actgttttgg aaaggctagc gtcatcaaaa cggtcgtcat caggacgttg acaatcacta
    11881 ccacaagtac ggcccagagc cagtattctg aatgacctaa aatcttccca atagcccaga
    11941 tagccataat tggaccacgc gttaccactt gcaagcccat agtaatcaac atttgaactt
    12001 gagtaatgtc attggtagta cgcgtcaaga ggctaggaat tgaaaatttc ttaatctctg
    12061 tctgcgagta atccaaaact cggttaaaaa tatcacttct cagcctacta gtataagaag
    12121 ccgccactcg ggatgcaaaa aatccaactg caactacgga caagaaggca agaaaggaca
    12181 ttcccatcat catgcttgcc ggctgccaca actcatctaa attagtttct tgactaccta
    12241 gcaaatccgt aattttcgag atataggtcg gcacttccaa ctctagatag accgaaaagc
    12301 aagtaaagag aatggctagt aaaatcatcc cccattcttt tctactaatt cttttggcta
    12361 atttctttat tctctcctcc tattcccttg atattttgcc tgtagttgac cgagaacctt
    12421 ctcaaaaatc agtaattcat cttcatcaat atcttccatc aactgcttgt ctatgcgttc
    12481 aaaaaaagcc ttaacctgtt gcatctgaga acgtgctttg tccgtcagac gaacaaactt
    12541 agcccgctta tcaacaggac tcgcctccaa ttccaccaaa ccattttgca ctatacgctt
    12601 aaccagatta ctagcaacag acttggtaat attgagttcc tgatcgatat ctttaatcaa
    12661 gaccaagtct tggtttttct cgcgattatc caaaaaatgc acaacctgac cttgcggccc
    12721 acccataaat tcaatgccgc aacgtttggc ttccttttgc accatcaggt gaatttgatg
    12781 accaaaacgc ttaaagacta acatcggttt acccataatc tcccccttct aaataaaaat
    12841 agttctctgg agaataatta aatttctatg agaactattt tcttgattaa aaaaatccca
    12901 agtgattttc tcacttagga tcatgttcta taggttaaat taaaacccat ctacgttcgt
    12961 ataaatcttt tggacgtctt cgtcgtcttc aagaacgctg taaagttttt caaaggtttc
    13021 aaggtcttcg cctgacaatt ccacttctga ctgaggaatc atttccaatt cagtcacttg
    13081 gaattcttca ataccagact cacggagggc aacgatagcc ttgtgaaggt cagttggcgc
    13141 tgtgtaaact gtgattgtac cttcttgtgc ttctacgtca tccacatcca catccgcttc
    13201 gagcaattgc tcaaagactg cgtccgcatc ttcacctcca aatacaataa cacctttgtt
    13261 gtcaaagagg taagaaacag aacctgaagc gcccatgttt ccgccgtttt taccaaaggc
    13321 tgcacggaca ttggctgctg tacggttaac gttagaagtc aaagtatcca caattagcat
    13381 agagccattt ggcccaaaac cttcgtaacg tccttctgta aaggtttcgt ctgtgtttcc
    13441 tttggcttta tcaatcgctt tatcgataat gtgttttggc acttgggctt gtttagcacg
    13501 gtcgataacg aatttcaaag ctgagtttga ttctggatct ggatcacctt ttttagctgc
    13561 tacatagatt tctacaccaa attttgcata tactttagag ttggctccat ctttagccgt
    13621 tttcttggct acgatattgg cccatttacg tcccattagg aatctccttt tttcacattt
    13681 taatctttct tattataaca caagtttttt tgattttcac tagaggaaat ggattttatt
    13741 agcaaatcaa gctaggatag cactttacct gctaagatgg tcttgccttt ctatctttat
    13801 caacagacac tcatccacat tcaaaaaaca aactagacca ttatctgcaa atagaaagtt
    13861 tcagccaagt ttgacaaagt cagctcaaat tactgtttga agtttgtaga tataagcgac
    13921 aaaaacaatc atactgcacc ttttgttgac agtctactcc agacatatca tagttcaagt
    13981 aaatactttg aaattcaaca gttcttatag gcgctattgt attctaagaa atcaatagaa
    14041 gagtttctaa gcaaacctct aatactcaat aaaaatcaaa gagcaaacta gaaagctagc
    14101 cgcaggttgc tcaaaacact gttttgaggt tgcggatagg gctgacatgg tttgaagaga
    14161 ttttctaaga gtataattta cgtgttccta agatggagaa gttagactag tacactggca
    14221 cttctaaacc attgctagca attgatttgt tcatatttaa tttcattttt tccataaatg
    14281 ggtattagat ataaacagca aaatatttcc gatacgtgtc attcttgaat ttccaatcat
    14341 ctaaaacaag taaaggataa tcaatcccct gtatatcaag gaattggcta ccctttttac
    14401 ttttttacac atcctgtttg atagactcat tttaacatca cgagcatact ccaatggaaa
    14461 tcgctaggca agagataaac tttcagatat ccgcagagag atcatcgcct ctttttgtcg
    14521 caagcattct cctctcctag tcattttcta ccttatcctc tacctgagga tagagagttg
    14581 ttccccaaat agaaatcgtc cgcttacgca ctagtggcaa atcggttttt tcataaaccg
    14641 tacgccacca ttcccaggca agcccggtac actctctaat tttgacagag agattacgaa
    14701 cattcccttt taaaggaata ctagtggtaa agtgagccgt caaatcctgc ccatttctgt
    14761 cccaagcctt aggagtcaag acttccttac cttgatgatc ataggataat tcatcccaag
    14821 taatataata ttgggcaaca taggcaccac tatgatccag cagtaaatct ccgtttctgt
    14881 aagctgtaac cttagtctca acatagtctg tactgttttg aaaggtcgca actacattgt
    14941 cacgtaaaaa agaagttgta taggaaatcg gcaagcctgg atgatctgct gtaaagcgac
    15001 tgccttcttg aatcaagtcc tctaccatat ccaccttgcc tgttacaact cgggcacccg
    15061 aacttgggtc gccccctaaa ataaccgcct tcacttctgt attgtccaaa atctgcttcc
    15121 actctgtctg aggagctacc ttgactcctt ttatcaaagc ttcaaaagca gcctctactt
    15181 catcactctt actcgtggtt tccaacttga gatagacttg gcgcccataa gcaacactcg
    15241 aaatatagac caaaggacgc tctgcagaaa ttcctctctg ttttaaatcc tctaccgtta
    15301 cagtatcttg aaacacatct cctggatttt taacagcgtc tacgctgact gtataataaa
    15361 tctgcttaaa attaacaatc tgaatctgct tttcacctga atggacagag ttaaaatcaa
    15421 tatcaagaga attccctgtc ttttcaaagt cagaaccaaa cttgaccttg agttgttcca
    15481 tgctgtgagc cgttattttt tcatactgca ttctagctgg gacattattg acctgaccat
    15541 aatcttgatg ccacttagcc aacaaatcgt ttaccgctcc gcgaacactt gaattgctgg
    15601 ggtcttccac ttggagaaag ctatcgctac ttgccaaacc aggcaaatca atactataag
    15661 tcatcggagc acgatcaacc gcaagaagag tgggattatt ctctaacaag gtctcatcca
    15721 ctacgagaag tgctccagga tagaggcgac tgtcgttggt agctgttaca gaaatatcac
    15781 ttgtatttgt cgacaagctc cgcttctttc tttcgataac aacaaactca tcgggtagct
    15841 gattaccctc tttgatgaaa cgattttcaa tactttctcc ctgatgggtc aagagtttct
    15901 ttttatcgta attcatagct agtataaagt catttactgc tttatttgcc atcttctacc
    15961 tcctaataag ttcctggatt gagttgcata aactcagact tgttcagcga aatcagccgt
    16021 ggttggacta agtgatccaa aatttcctcg tacaattctt ctgagacatt gcgtcgccgt
    16081 ctggctagat aagaagtcgg aataaccgta ttatccaaca taaatacctt atctaagtca
    16141 atcaaggttg gtcttgtaaa aggattacga gctagatccg gctcttctat cataaagttc
    16201 ttgaccaaac gtctggtcaa gagagctggt ttgaaggtct gatttttaac caactctttg
    16261 tttttagtca tgctgttgtc aatacagata tacatatgat tcttcacagc caaatcgcta
    16321 ctaatagtcg gaaaaggcaa ataaagagct acaacatctc ctctcttaat caagcaagag
    16381 cacccccttt tctcctaatg taacatagac aggattgacc aagtcttctg attgactcag
    16441 aatttccaaa gtttgagttt ggcgcgctgt caatttagta gcatcttgtc tcttcaatac
    16501 aaaatgcttg tcgccaataa ccttgacaat ataatccttc tccaaagctg actggtaaat
    16561 ccacatcaga tgttgtctgt cctgagaact caagagagaa ggattttcaa gcctcccgat
    16621 agtctgataa aaatcaaaaa caggagctaa ctcctgccaa tctgattggc tagttgtcaa
    16681 ggctagaaaa agggctttgc gagctgatac ttcttggtta gccttgagag ttactttccc
    16741 ctccaagttt tttagaaatc gggaaactcc agaaagcaaa tttttctcta actgcgagaa
    16801 ataaaaacct ttcgttccca gacataagtc tttcatgtcg ctttctctag caaataagag
    16861 ctcaaacatt tgatagtaaa agaaaaatat ctggcactgg gtcgcgctca tcttttcctt
    16921 atcggcttct ttttttaacc agagcaaggg cgacaggtaa ctggattgag acatttcctc
    16981 tacctcctac tcttttttaa ctggagcatc tgcactagct gccacttctt ttgactggat
    17041 actttcccac tggttaatct cctctgagat aagaccttcg catgtcttga caaatagggc
    17101 aaaagccttg gtctttcctg catatttctc cgtttggcat tgatagagga atttttcttt
    17161 ctccaggagt tgcgcagttt tttggtaaga aatccaattt tcctttgcat tatacaaatt
    17221 gataatcccc tcacacagca agccgagact ggataaggca accgaaatca aacggtagcg
    17281 atcacctggc ataggaatag cacaaaagac agctatgagg aaacctgcca cgatttctgt
    17341 tatttttaat accttatagc gcctacgatg ttgaacgctt ttctttaaaa aatgagctat
    17401 ctgtacgtct aatcgctctg tcaggtacat ttcttctggc gtcatattcg taactccttt
    17461 catttacttt gataaccata gtataacata tttgaatatc aaagtatagt aaaaactgga
    17521 ctataactta ttagacttcc tgcgaaacaa aatatggtat agtagttcta tgaatgatga
    17581 agcaagtaaa caactaactg atgcacgatt taagcgtctt gttggtgttc agcgtaccac
    17641 ttttgaagag atgttagctg tattaaaaac agcttatcaa aaaagtcgga caagctggta
    17701 aaatcttggc tgacagtaat tatcaagggc tcatgaagat atatcctcaa gcacaaactc
    17761 cacgtaaatc cagcaaactc aagccactaa cagttgaaga taaagtctat aaccatgcgc
    17821 tatctaagga gagaagcaag gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt
    17881 tttcaacaac ctgtcgaaat catcgtaaac gcttcggatt acgaatgaat ttgagtgctg
    17941 gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctattata tatatgcaca
    18001 gacatatgaa atctttttga tacagccttc tttttacata gagagacagc agttatgata
    18061 tatttatcca ttcacaacat cttctgtacc aatactacaa taattggtac attttgatta
    18121 cacaatcgtt tcaaaaaata tgatggaaga ctgagtattt tatagtaatt cccttcctat
    18181 caactagatc aaccagttga gtgttgaatg ttgaaacacc ctagcctcag caaaggacta
    18241 tgatcagcat ttcctagcca ataggacgct ccaaatcacc tattctaagt ccatcactag
    18301 cttggtcagt aagatacaga ctgcaaggca agactgacgt ggttgatgag attttggaag
    18361 agtattacaa tcatagattc accagtagac ttgaccgcag atttaactat agtagattga
    18421 aactagaata gtacacttct gcttctaaaa cattgttaga aatcgatttg actgtcctga
    18481 tcaatttgtc ctgttctcgt ttcattttac tatactaaaa aaccacctga aacaaatcaa
    18541 gtgactagag gataaaaaca aagactgggc aaaaagtttt gaaaagcaaa aaacgcataa
    18601 tatcaggtct tgaaaaacct tgatactata cgtttgattg tgggaagatt gacttcattt
    18661 tctcctgaaa ttgaagtttt ttctagtttc caggtataga cgaattcaat atagccatct
    18721 cgctacaatt attttaacca tcgaaaaaat ttttcagaat ttcaaaaatt tcattttgat
    18781 ccaatgaaat gtcattattt tgaagaatag ttgacttcac aggctctaat tctatcacaa
    18841 agcggtgcat gtctgctttt accgcactac ttgatgttat agccgataac ttttcatctc
    18901 ctagcaagga agctgtcaaa cgggtaaggt cattcaaatg ctttttaatg gacttactta
    18961 aaccttgagc acctgtggca gagcgctccc tcatatccag ccaagcttta gccttaaaaa
    19021 taattaatac tcaatgaaaa tcaaagagca aactaggaag ctagttgcag gctgctcaaa
    19081 acactgtttt gaggttgtag atggaagctg acgtggtttg aagagatttt cgaagagtat
    19141 agaccacaat tactcaatac cgaatacccc tgaatggttt cttttacatg caccaatata
    19201 ttataataat cttcatccaa taataaggct gataaactag catcttggtc aaaatataag
    19261 ggaatttctc gaccgtcctt ctttaatgga tattctggta agatactaaa tagttcaatc
    19321 atagaaggaa actcagaatt agttgttgta aatcgaaaaa gctgcgcttt ctcatctttc
    19381 tgacttcctt gatactctcc caattctaaa aaatgattca aggtagtata aaattcctta
    19441 ttttttactt catcaatgat gaccatatca taatcttttg ttgtgcgact tttaaatcct
    19501 tgcgaatcca atacgataga ggtagcagtt cccccaatca gaacataata gttctgaaaa
    19561 tccgcaaaca tttcttgaaa aataactttc gtattagctg gcatcatctt ctcccagata
    19621 ctgtaatatc atattttcta gtgcttcact ctcttcctct atacgtgggt catcatcatc
    19681 ttttaaggtc aaataaagag aaatcggatc tacaaattgt ttatcatgat tatttttaaa
    19741 atcattccaa aactcagata caaaaggacg atatttccat atctctagca tctttcattt
    19801 taaaacatgc tgagaaagtg gcaaggataa ctgattgaat tttctctgcc atatgacata
    19861 gctaatattt tcatccgttt cagctaaaaa agttgaatgc gacaaagcat aagcaccacc
    19921 atatagaagg ttagaaacag attttatctg ctttatatcg ccatctggca ataaaatccg
    19981 ttttttgatg ggattaaata aacatgacac ggattttaag aataattctt tctttgacac
    20041 cgtatatgtg taaagcttat tttgcttgtt taaccaatat aaagctttaa aagtcctcaa
    20101 acacctataa attgttgagt ttggaagtcc agtgacttgt gaaagcaaat caacatctac
    20161 tactttttga ccttttgtca ataaaaaggc aatccacgtt aattgttcgc taggtgttaa
    20221 ttccttaggg acttcagtat cattcgcatt gagtactagt cccaatggag ggaagaagag
    20281 gtttccctta aagtctacaa acggaactct agcttgaagt aattgctttt tttcactgtc
    20341 tgataacttc gaaaacacca aaacaacatc catattggct ttttcaccca tagtgcgagc
    20401 ctgagtaaca aatgaactca aactccccct tctcttttct ttaatcaata aaaatgattc
    20461 cttatgaaaa gtcatttgag tatactcaaa cctttttaca aaactgattg gtaaagtcaa
    20521 agtcagttcc tcaatactga catcaacata aaaggattga aaaattgaca ttatcttttt
    20581 gacattcatg ttttttcctt attctatcat ttttattata ttttatcatt cggtataata
    20641 aaatataagt tttttgataa aataaccaaa aactactcgc tatcccctct cacattaccc
    20701 tacatatcgt ttgacatgcg actgatagtt caggaaaact tcaaggagaa cttttctctc
    20761 atccactatg caggacttac tatttcactt ctactcctat aggctcaatt tgacactttt
    20821 ctttttcgaa ttgctcatat gcctcctgaa tagtgagttc gatttatcca aatttatttt
    20881 tgtctatttt gatgaatact tccacgaaga tagtctgaaa atgaatttgc accaattttc
    20941 cttctctttt taagcatatt attccagata tgattgccct cttgttcagt aataaattac
    21001 gctttaaacg cttcaatcag tatatctcct gttgtcatat gttctaaaga aaattcttct
    21061 acatatgatt taacatctct taggttatta cttcctaata tcccattatg ctttttcgct
    21121 aaggaaatag aagccgcttc tcccttacca ataatcttgt tattatcatg atttcttgtt
    21181 aaatctctat ataatgcgta ttcttcagtt ccaatgtcta tgctcacaat ctcagctgaa
    21241 cccttagcta ccaactgatc tatcctagat tttaaatggg gaattgtagg tatattgatt
    21301 tcatcataca cctcttgtgg aataacaatt ttacccgaat agagcttttc taaaagatgt
    21361 tcagtgccaa cccataaaaa tactgaaata caatctgcat caataaatac acgactagtc
    21421 aaacgacaac tcccccctct tcatctagcc catatacaat atcatatctg aaagcatcta
    21481 gtaacagttc ctcatacttc ccttgcgaaa ttctgttatt ttctaaaagt tgttcagttg
    21541 aattaatata ttatcctaat accatttcct ttttactttc tgacaaagga cgatataaac
    21601 ttgtatcata gcctaatctt gaagctgtct ctataacact aatatccata tttttaattt
    21661 cttctgcatc aaggtatcca tcattcctca atctatataa catagcttta tgactgatac
    21721 cataaaactg acccaatttt ataatatctt ctacttcaag atgagttcta ttggcatttt
    21781 ctctgatttc ctcaaccatc ctatacagtg aagatgggaa aattaaaaaa taagaagcaa
    21841 gctgatccgc ttttctttca gtttcatctc cttcaccaat caagataaga ctgactgaac
    21901 tcttcttcac ctcatcataa taaagatgat acagttcatg tgctaaagaa aatctttacc
    21961 ttcctaatga catgtctgaa ttgactgcaa tgagactgaa ctgagttcct ttataacaga
    22021 ctccgctaat attctttccg agtccataaa ataccagcgt caaattttct atcttttgta
    22081 ccaatttaaa aatatctatc ggcgcttcac catcagctcc caactttttt ctaagatttg
    22141 aagctttatt acttaaatcc attcgtgata ttcctttcac ctttttccat tttggcaatc
    22201 atgctttgat tcaaagacaa atataaagac aaatattcag caatcttatc ttgagtaaga
    22261 tttgaagata ttctaagttc ttttattctt tttccaacat cacatacatt tatcacatac
    22321 atttatcaca tacatttatc atattcatac acctctcata aaaagaatag cacacttttg
    22381 tcataatttt ttaaataaaa aaattatgac aaaacaagga agcaatttat tgatgctgct
    22441 taaaaatcta aaattgatga gttcctatca aaaatcgtat cttcaacctc aaaacagtac
    22501 ttaaagctat ccgactcggt ttacattgtc aaatttagat tttatttgag cataactttc
    22561 tagtttgctt tctgattttt gtttaatata gtagcaaaca ttgaaggaaa tatctccaca
    22621 agaaaacgca tactattaag ctttttcaag acctaataat atgcgctgtt ctgatttgaa
    22681 agacattcca ttattatttt actgtaatca agccatctgg ctctactgtg aattctggcc
    22741 tgtctgccag tgttccgtct ggtttgaggt agtaccagcc tgttccgtcc gctgactgga
    22801 taaaggcatt tgataccatg gcgccttctt tagcgtctaa gtagtaccaa gtgtccttgt
    22861 acttgaccca gcctgtcttc atggcacctt cttcgttgaa atagtaccac ttatcagcga
    22921 ttttcttcca gcctgtagcc atttcgcctg agttgtcgaa ccagtaccag ttgccgtctg
    22981 tgtgcttcct ccagcggtct gcaagcatat agcctgaact gtcaaagtag taccaagtgc
    23041 cattgatttt ctcaaacttg tcttttggat aagagccgtc tgaatgtacg taccagtagc
    23101 cagtgtcatt cttctgccag cctgtttcaa tcgtcaagcc gttctcaata tcatgcttaa
    23161 actgctcacg gctaatgccc catttagcaa gatatggata agggtcaacg tggtctgagt
    23221 ggttgtttgg ttggttattc gtgcaatact cgtgcgtttt aattccagct aaactccctg
    23281 tatcaagcgt tttcggcaaa cctgcttcat ctgctagatt gcgtaagagt tcgatataaa
    23341 ggcggtagtc cgtcatgaac tcttctttgg ttgaatggct ttcaatcagt tcaaccgctg
    23401 cataggtctc agcattccaa ccgcccccaa cgtcccaggc accattatca acaggtccta
    23461 cctgcatgat gcaaccgttc ccaacaatgt gcgagaaaaa acctaattct gggtctttcc
    23521 gccagtgata atccgcttca ttctgtacgg ttgaatgcgg attcccagtt gagtgtgcgt
    23581 gtacttgcct atatggttgc acgccgactt gaggcaaatc tgttcttaat ttactcacat
    23641 taatttccat attctactcc ttatcaatta aaacaactca ttttttacaa tccagaacca
    23701 gaaactcctt tatttctacc ttacaaagaa gacaatctta gtcacgatta ggcttgtaga
    23761 tagaacttca aaacgcacta ttttgacact gtaaatagga ctgacaaggt ctgcattcta
    23821 tctacaataa caccccagac taaaaagctt ttcaaaagta tatttttaca gtctctatat
    23881 gtccttttca taaatactat actttattat atcatataaa agaagtcaaa agtctgttaa
    23941 actattttca acaccaaact aaagacgaga acacaagttt tttcgatttt cactagagga
    24001 aatggatttt attcagtaaa tccaactagg attgcacttt ggttgccaag attgccttgc
    24061 cttcttttat caagggatga cggaataatg agaagtacag ttgaactgtc atggcaaccc
    24121 agataagagg ttcacaaagg ataacaccct tatatcctgc ccaaggaata atcaaaacca
    24181 caaaaacgat ttttccgatt agttcaataa agctagaaac tagaggaagg atcttttgcc
    24241 ccaagccctg caagcaattg cgataaatca acaagaggct caaaatggga taaaaggttg
    24301 aactgatttg cagatagaga cttccatttt ctatcaagta accatctgtc gaactagcca
    24361 agaaggaaac caaagctgga ctggcaaaaa agaggaaaat acaaacaaaa actgcccagg
    24421 atatacttaa acgactgccg attcgaagac cttgaacaat acggtcaggt cgcttagctc
    24481 ctagattctg agaagcaaag gtcgtcattg atgcagaaat agcggtcata ggaagaaggg
    24541 caaaggtcat aatgcgtcga gctgccgtct gggcactaat aatcactgca ccaaatgtat
    24601 taacagaaaa ctgtaaaatc acactgccga tagatacaat tgaactcatc aagcccatag
    24661 ccaaaccttg ctccaagaga tccgcgtaca agcttttgtc ccatttgaaa tgtttaaact
    24721 gtggcaagag ttctggcaca cttttacgaa tataataaaa gcagagaacc gctgataaac
    24781 cttgcgaaat aatggtagca agtcctgcgg attgaactcc cagatgcaat tgcgtaataa
    24841 aatagagatc cagaaccaca ttaaccaagg cagagaaaat cagaaatccc agggctgcta
    24901 gactgtcacc aatagaccgc aacaagcctg caaaaagatt ataagcaaag ctgacaccta
    24961 cacaggtcac aatcatagaa atatattgat aagattgagg aagaatttct gcaggagtat
    25021 ctaagtattg caagagagga tacaagccaa gaaagcccag caacataact agaatgctca
    25081 aaagagcacc taaaatccag gtggctgcta ctgcttcctt gattttagtg aaattccgag
    25141 ccccataata acgagcaatg acaatcccca tgccattgcc aacaccaagt gtaaagccta
    25201 caatcaggtc aaaaatcgct gtcgtcgctc ctactgcagc caaggattct tgaccaagaa
    25261 atcgtccaac aatcaagaca tcagcagtgt tatagagctg ttgaaaaata tttgatagca
    25321 agattggaaa ggtgaagctt aagagcgagg gaagaatcgg accatgtatc aggtccactg
    25381 ttcgtttttt attcataaag ctattatatc agctttccag aggagcgaca agaaacagca
    25441 aacttatata tagcggaatg aaataaggac agtcaaatcg atttttaaca atgttttaga
    25501 agtagatgtg tactattcta gtttcgatct attatagttg gttgagtttg gaatagtacg
    25561 ctgtagctgc taaaacattt ctagaaatta atttgacttt cctaataaag ttgttcatat
    25621 cttatttcaa gctactatat ataatatata atctaaaaac ttgaatcaaa aatcgaaaaa
    25681 gtagcggatt tctccactgc tttaacagct tattcttcaa tttcgatttc aagttcatcg
    25741 cctaagtcaa gcggaacttc ttcattcact gcttcttctt tctttggctc atcttttttg
    25801 ttttcagtat cttgttctga aacttcttct ccatcaatca agccaaattt agaacggact
    25861 tgcttatcaa tttcatcaaa gatttctggg tgctctgcca agtatttctt agcattctca
    25921 gaaccttgcc caattttttc atctttgtaa gaataccaag cccctgcttt tttgataata
    25981 tccaaatcgc ttgcaatctt caaaagctca ccagtcttag aaattccttc tccgtacata
    26041 atttcaacta cggcttcctt aaacggtgga gctaccttat tttttacaac cttaatctta
    26101 gtttctttac cgacattggt ttctttttga tcaccagttc ccttaatttg tgtattacca
    26161 cgaacatcca agcggactga agcatagaat ttcaaagcac gtccgcccgg tgttgtttct
    26221 ggatttccaa acatcactcc aactttttca cgcaattggt tgataaaaat ggcaattgtt
    26281 ttggttttat tgatagaggc gccaagttta cgcatggcct ggctcatcat acgagcctgc
    26341 aaaccaacat ggctatctcc gatatctcca tcaatttccg cacgaggaac aagggcagca
    26401 actgagtcga ctacgacaag atcaactgca cctgagtcaa tcaattttcc cgcaatctca
    26461 agaccttgct ctcctgagtc tggttgagac aagagcaatt cgtcaatatt gacaccaagg
    26521 gccgcagcat aagctggatc aagggcatgt tccgcatcga taaaggcagc aatcccacct
    26581 tctttttgcg cttgtgcaac tgcatgaagg gcaaccgttg tcttaccaga tgactctggg
    26641 ccatagattt cgatgatacg tcccttagga taaccacctg agccaagggc aatgtcaaga
    26701 gctaaagaac ctgagctcat cacttgcacc ttttgctccg cacgttcacc caaacgcatg
    26761 attgatcctt taccaaagtc tttctcaatc aatttaagag cgtcattcaa ggccttttca
    26821 cgttctgccc caaatttttt tgaaatttct tctaattttt ttggtttttt cgccattcta
    26881 ttctcctaca ttctaatgat cctcagacct atctactatt atatcaaaag ttagtcactt
    26941 aataaagcct tgcgaactag gttaaaggca tgcataaccg caatgtgacg tacatctgct
    27001 cggcttctgc ctccaatatt aaccttgata acctcagttc cttgatcttg cgccaagcct
    27061 atgaagactg tcccaactgg gtgtccttct aggctatctg gtcctgccac tccagtcaaa
    27121 ctaattccaa aatcagactg ggtcttgctt cgtgcctgct cagccatctt ctgagctgta
    27181 aattcagaca ccacaccatg ttcttccaaa ttcttggcag gaatatccaa catccttgat
    27241 ttttcctcca agctataggt cacaaaacca ccctcaaata tacttgaaac tccagaaaaa
    27301 ttcgccacgg tagcttggaa aagacctgcc gtcaaactct ctgcagccgc gatggttttc
    27361 ccttgccttt tcagttcttc taccacaatg ctggctaaac tagtttcttc cccataacca
    27421 tagcaaaagt ctcgtaaaga aattccttcg aaagtctggc agtccaagat ttgattttcc
    27481 aagatatcca gcgcttgatt cgcctcttct tgactgctag cctttgttga cagacgtaga
    27541 gtgacttctc ctgtcttggc ataaggggcc aaggtaggat cgatctgatt atcaattaaa
    27601 tcagccaaaa tcgtaaccaa ctggctctcg ccaatcccaa agaaacgaag aactcgggaa
    27661 tacagcttgc tccctgtcat caacttgggt agaagttggt ttaagaccat gggtttcaat
    27721 tcacttggcg gacctggaag gacgacatag gtcactccgt ctacttctaa ttttcctcca
    27781 acagccagtc ctgtttcgtt tggcagtgga atcgctcctt ctacaatttg agcttgtctt
    27841 tcgttattcg gtgttcgggc atagtctggt cgcagggcaa aaaagatatc caacttctcc
    27901 tgagcctgag gatcaaagac taatgctttc cctaaaaatt tagctagggt ttgtttggtt
    27961 aggtcgtcct cagttggccc caaaccgcct gtcaaaatca ccagactgct acgttgactg
    28021 gcaatctcaa gcaaagacaa gagacgaact tcattgtctc ctacagccgt ctgaaaatat
    28081 acatctaccc caatctcagc tagtttttcc gacaaaaact gggcattggt gttgacaatc
    28141 tgtcctgtca aaatctctgt tccaacagca atgatttctg ctttcatgtt tcctcctacc
    28201 tatctattcg tatttttttg aaaaaatcgc aggaattttc ctacgattga tttttttatt
    28261 tgtatcaaaa gttaattatc ttcatcacca acaggtgctc tgccaaataa atcttcaaat
    28321 aaaaccgcat tggtttcaag ctgagtaact tcttcttgtc ccaaagaacg tcggagtata
    28381 ttttgcattt ccaacatatg tgctctcgaa acaatctggt aagaaacacc ttgaagtatc
    28441 tctccttcac cctgcaactg ctgagtttca atggttttaa atgaatcttt atagcctagc
    28501 aagttaggga tactttttgc agacaaatca atattggtct gcatattgtc actcaaagct
    28561 tttagaatct cttgataatg accaatgcta tttaaactga gagctttttc catgactttt
    28621 tgaataactt cacgttgacg tttttgacga ccataatccc cctcaggatc ttggtaacgc
    28681 attcgtgcat agactagggc ttcttctccc ccaatatgtt gctccccaac accgatagaa
    28741 atggtattaa attcttcttg gtcactgata gaaattggga aacctaggat attattgact
    28801 gtaatacctc ctactgcatc cactaatttt tgcaaccctc tcatattgac catcacatag
    28861 cgatcaatat ggatattcat cattttttga atggtttcta tagcaagctc tgctccacca
    28921 tctgcatatg ctgagttcag tttcgcttca tgagcctgac cattccctga ttcaatgcgc
    28981 gtcagaatat cccgctctaa actcatcatt gttgtttttt tcgttttagg attcactgtc
    29041 atcaagatca tgctatcact tctaccgacc caagtttcag ttcgttcaac atttccggtg
    29101 tccactccca ttaacagaat ggttagaggt tcagtcgctt caataacctt ggtttcttca
    29161 ccgatttttt tataggtttt agctaaggtt tctgtccctt gttgataaat agtataagca
    29221 aaaacaccta ctcctactac agttacagaa agtaaagcta gcaccattcc aataattttt
    29281 ttaaccatat ttctactaac ctatcagttt acccatcaag taaacatcga taaatttccc
    29341 ttcttctata tatgccccac gctcttggct accttcaatg acaaagccat gcttttgata
    29401 aagatggact gctgcttgat tacgagtttg gacagtcagt tggagacgac gcagaatgcc
    29461 acttgcttgt gcccactcta tcgcttcttc tagcaacaaa cttcccaagc cattattcca
    29521 atatcttttt ccaatcacaa tgaagagatc tccaatatga cggactctct tacgctgatc
    29581 agctgtaata tttacaatac cagcaatttt gccatttaag aatgcaagta aggttatctg
    29641 attgtccgaa ctagcttgct tgttgaggaa tatttccatc tcctcactag tcaagagaat
    29701 accatctccg tctaggctgg taaagtctgt ctccaaactc acacgattta aaaaggccac
    29761 taattcagct gcatctttgg gctctgcttc cctaatgagc aattcatact ccatattgaa
    29821 gctcctctaa caatttctca gcacgcaaac cctttgcctg aaaatttaaa cggcgtccat
    29881 ctgcttcttt tagaatttcc aattctaaat aagcatctgg caaggcatct cctaagagat
    29941 ttccccactc aataacagtc acgccgccac caaagataaa ctcatccaag tcgatagaat
    30001 cagcatctcc ttcaatacga taaacatcta ggtgataaag tggaagtcga ccttcatact
    30061 ctctcacgat agtataggtg ggacttttaa tcatttgaga aatctgtaat ccttttgcaa
    30121 gtcctttagt aaaggtcgtt ttacctgcac ccagttctcc agttaagatt aaaacatcat
    30181 tctttgctaa tagatggccc aaacgctccc ctaaggcttg caactcttct tcattttttg
    30241 tgtacatact cttattatac caaaaacttt tcttttgtgt ctattttcct actaaactta
    30301 tcatcataac atccataaaa aacaggcttt ctctaaaaga aaatgagcgt aacaatgacc
    30361 aatacaagat ctcggaaaat atgaccataa aaggaaactt ccttcttaac cgaatttggg
    30421 acaagatagg ctgcaaaaaa caagcccagt ccaatataaa tcagaagtga gacaatggtc
    30481 attggatttc ttaagaaaag aagtgttgct aaaatagtca ccaacactgt cttttttctg
    30541 tccagcatag caagaaaatc gcgcacgtat tttttcaagg gtaaaaaaat cagcaaatct
    30601 agcccaaata ggaaaaagaa ggatggcaat aaaaagtcaa ctaattcttg ctgcagcgta
    30661 tttttgatga acaagttatc tgacaaaaca agaacagctc ctaacaaatt aattaagagt
    30721 aacatactgt aaaaaagctt caccgacttc ttactggcta ggacactatg gacttcttgc
    30781 ttacgggtat aaagataatt tactccagca cagattcctg aaacgaaaac catgcttccg
    30841 atgaaaaaag ctgtactttg tttaaaggac aagatgcatt ccttccatag gaaacagcta
    30901 ctcaaactga tttgaattaa agctaacaaa aataagattc tcattgattt catcttctct
    30961 ctcccttcct accaatcatt atactaggag aaaagagaga actgtttcta atcttctcaa
    31021 atgtctcttt aagacgctaa acaaacacta gagactaata ctcaatgaaa atcaaagatc
    31081 aaactaggta gctagccaca ggttgctcaa aacagtgttt tgagattgca gatagagctg
    31141 acgtgatttg aagagatttt cgaagaatat aaatttgaaa tcatgaaaat ccgtcaaacg
    31201 ggtgattgtt ttgtctcgca cctcacggag cgagacggac tcagagtcac ataattataa
    31261 ggctgatagt attaatctaa ctatcagcct tacaggttat ttaacgtttc agaaaaacta
    31321 taatgtcaag attaactaaa cagtatctag ttccttcaaa taattttcta tcttcatcaa
    31381 cattaaagga ttgttataaa tcttacataa ctctcttgct tctatataat aatttttgac
    31441 ttgttctctg tctagaaatt tggctccagc atttcctaca agaataagta gaggagccaa
    31501 ttggtagctt gtctgtcttt gtttacagag ttcaatcgtt tcaagagctt cttggatggc
    31561 ttcattatat ttttcctttg atactaggta gtgagcgtag ttgtaacgaa ctctgatgta
    31621 gccaaataaa aactcttgat ggtccaaatt ttttgtctga tacaactcta ttaaatgaga
    31681 gtagtttgcc tcatattctt gttcacgacc cactaaggaa tagaaattag atagagtatt
    31741 caacgccttt aaataaatca gagtatttga agagactttt aataatatat tttccaatga
    31801 cgaaattgcc tcacacttac tgtcatattg atagaagtca attatagatt taatccattc
    31861 aaggtaagtt cggtcttcta atgttagaaa agtgcttcgt tctacctcta ttttataaag
    31921 atattctaaa tcgtcataat ttctgtcatc taataggcga gcagatagat gtttgaaatt
    31981 agagaggtta gacttaactt cgatttgttc attgaaaaag taatccaaag ggacttcaag
    32041 tcgttgagag agtttgaata acaagtctgc ggagggaata aaatgacctc tttcaatttt
    32101 actaatctgg ctttgttcac aaattccttc tgcaagagtt tgttgggaga gtctcaactc
    32161 ttttcttttc aatctgaatt tctctgcgag tgtattcatt aaagataata aaccttccta
    32221 tttgcttaat ttcattataa agtttttagt tcaaaatagc aagttaaagg aataaataat
    32281 tctttatggt aataaacaaa aaaaaaagtt aaaaattaca taaaaatact tgactaaata
    32341 aaatatatct gttagaataa aaacaaggaa aaagaaaggg gtttcattgc atgagaaaaa
    32401 aacgtggaat taaaaaatta gttacatttg cattgctagg tgtttttatg tttagtaata
    32461 caattcctta ccaacagttt attcagaaga ataaacaatt ggagattcga gtgcaatcgc
    32521 aaaagaagtc caatggtctt gatgttggga aggcggatta aaacccgata ttttgcacga
    32581 gaatgagaga attataattt atctgaaaaa acgaaaaata tagagcaact tatttaactt
    32641 tttataagga ggagttatta cttatgacca actttaattc aaacgaaaaa ttttgtggaa
    32701 aaagtttaaa atcattgtct gctgatgaaa tgagtttaat ttatggtgca tctgatggtg
    32761 cagagccacg ttggacgcct actcctatta ttttgaaatc agctgcagct agctcgaaag
    32821 tatgcattag tgcagctgtt tctggtattg gggggctagt tagctacaat aatgattgtt
    32881 tggggtaaat taaaatgaaa aatgattttg taatcggaaa gagtttgaaa gaattatctc
    32941 ttgaagaaat gcaattggtt tatggtggca cagatggtgc tgaccctaga tcaacaatta
    33001 tatgttctgc tacattaagt tttattgcat catatttggg gtcagctcag actcgttgtg
    33061 gcaaagacaa caagaaaaaa taaaatattc aattttaaaa ataaaagttg ctctttattt
    33121 tcctctctcg taactaataa ttttgagaga ggtttttttt taaaacaata gtaagtcgta
    33181 taggtt
//
LOCUS       DAVKMU010000021        32369 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKMU010000021 DAVKMU010000000
VERSION     DAVKMU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.021"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..614
                     /locus_tag="VW343_001125"
                     /pseudo
     CDS             1..614
                     /locus_tag="VW343_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(742..1764)
                     /gene="cbpF"
                     /locus_tag="VW343_001126"
                     /pseudo
     CDS             complement(742..1764)
                     /gene="cbpF"
                     /locus_tag="VW343_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1783..2604)
                     /gene="cbpG"
                     /locus_tag="VW343_001127"
     CDS             complement(1783..2604)
                     /gene="cbpG"
                     /locus_tag="VW343_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEU9755686.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2685..2924)
                     /locus_tag="VW343_001128"
     CDS             complement(2685..2924)
                     /locus_tag="VW343_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755687.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3074..3340)
                     /locus_tag="VW343_001129"
                     /pseudo
     CDS             complement(3074..3340)
                     /locus_tag="VW343_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3365..3676)
                     /locus_tag="VW343_001130"
     CDS             complement(3365..3676)
                     /locus_tag="VW343_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9755688.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLT
                     S"
     gene            complement(3601..3843)
                     /locus_tag="VW343_001131"
     CDS             complement(3601..3843)
                     /locus_tag="VW343_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9755689.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(3844..4476)
                     /locus_tag="VW343_001132"
     CDS             complement(3844..4476)
                     /locus_tag="VW343_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9755690.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4490..5485)
                     /locus_tag="VW343_001133"
     CDS             complement(4490..5485)
                     /locus_tag="VW343_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9755691.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDQDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5482..6180)
                     /locus_tag="VW343_001134"
     CDS             complement(5482..6180)
                     /locus_tag="VW343_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9755692.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6258..7268)
                     /locus_tag="VW343_001135"
     CDS             complement(6258..7268)
                     /locus_tag="VW343_001135"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9755693.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(7252..8259)
                     /locus_tag="VW343_001136"
     CDS             complement(7252..8259)
                     /locus_tag="VW343_001136"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9755694.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAESTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8246..9199)
                     /gene="mvaD"
                     /locus_tag="VW343_001137"
     CDS             complement(8246..9199)
                     /gene="mvaD"
                     /locus_tag="VW343_001137"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9755695.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(9181..10059)
                     /gene="mvk"
                     /locus_tag="VW343_001138"
     CDS             complement(9181..10059)
                     /gene="mvk"
                     /locus_tag="VW343_001138"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9755696.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10234..10512)
                     /locus_tag="VW343_001139"
     CDS             complement(10234..10512)
                     /locus_tag="VW343_001139"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9755697.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSAQVPWQ"
     gene            11175..11513
                     /locus_tag="VW343_001140"
     CDS             11175..11513
                     /locus_tag="VW343_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
                     /protein_id="HEU9755698.1"
                     /translation="MTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANV
                     TAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLKTFIHNSIWGNLSLFPTK
                     RYSQRKHFRL"
     gene            11413..11883
                     /locus_tag="VW343_001141"
     CDS             11413..11883
                     /locus_tag="VW343_001141"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755699.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKSNR"
     gene            12042..13274
                     /locus_tag="VW343_001142"
     CDS             12042..13274
                     /locus_tag="VW343_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9755700.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEIVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTLFLVLSPFSLI
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(13392..14390)
                     /gene="cbpJ"
                     /locus_tag="VW343_001143"
     CDS             complement(13392..14390)
                     /gene="cbpJ"
                     /locus_tag="VW343_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9755701.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKELEIKTQDSVGRKYGEKRENSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(14409..15371)
                     /gene="cbpC"
                     /locus_tag="VW343_001144"
     CDS             complement(14409..15371)
                     /gene="cbpC"
                     /locus_tag="VW343_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9755702.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLDPSN
                     GDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(15469..16158)
                     /locus_tag="VW343_001145"
     CDS             complement(15469..16158)
                     /locus_tag="VW343_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9755703.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLETLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(16170..17594)
                     /gene="gndA"
                     /locus_tag="VW343_001146"
     CDS             complement(16170..17594)
                     /gene="gndA"
                     /locus_tag="VW343_001146"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9755704.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(17670..19064)
                     /gene="mapZ"
                     /locus_tag="VW343_001147"
     CDS             complement(17670..19064)
                     /gene="mapZ"
                     /locus_tag="VW343_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9755705.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(19077..20234)
                     /locus_tag="VW343_001148"
     CDS             complement(19077..20234)
                     /locus_tag="VW343_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9755706.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFDEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(20319..20699)
                     /gene="rnpB"
                     /locus_tag="VW343_001149"
     ncRNA           complement(20319..20699)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW343_001149"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(20720..21049)
                     /gene="gpsB"
                     /locus_tag="VW343_001150"
     CDS             complement(20720..21049)
                     /gene="gpsB"
                     /locus_tag="VW343_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9755707.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(21119..21646)
                     /locus_tag="VW343_001151"
     CDS             complement(21119..21646)
                     /locus_tag="VW343_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9755708.1"
                     /translation="MATALVLGYSTFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            21712..22308
                     /gene="recU"
                     /locus_tag="VW343_001152"
     CDS             21712..22308
                     /gene="recU"
                     /locus_tag="VW343_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9755709.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            22305..24464
                     /locus_tag="VW343_001153"
     CDS             22305..24464
                     /locus_tag="VW343_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU9755710.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(24631..29949)
                     /locus_tag="VW343_001154"
     CDS             complement(24631..29949)
                     /locus_tag="VW343_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9755711.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQ
                     NDNAGKPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYD
                     KDGWFWEYKTPGNSTWYKGNRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTL
                     PAAVNDHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKV
                     TYDTIQSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKIN
                     ETTAEYLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLL
                     SSISFLGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVS
                     TDKLAAGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFP
                     EYTKELPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFG
                     SQAQNPFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDF
                     KTLIEKAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDA
                     AYDLAHGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRF
                     AIEWGHGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAA
                     NYPLLGGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDN
                     GSTYKWTPEMRVELVDANNNKVVVTRKSNDVNSPQYRKRTVTLNGRVIQDGSAYLTPW
                     NWDANGKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVK
                     DGKITLDLLANQPYVFYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEI
                     VKSQGANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKE
                     VTTYTNKSLALNYVKAYAHNTRRDNATVDNTSYFQNMYAFFTTGSDVSNVTLTLSREA
                     GDQATYFDEIRTFENNSSMYGENHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRT
                     HLSEKHDPYTQRGWNGKKVDDVIDGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYR
                     VTFEYEAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTG
                     AETGDTWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALK
                     NYLPTVAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTA
                     LVADDFASLTAPAQAQEGLANAFDGNVSSLWHTSWGGGDVGKPATMVLKEPTEITGLR
                     YVPRGSGSNGNLRDVKLVVTDESGKEHTFTATDWPNNNKPKDIDFGKTIKAKKIVLTG
                     TKTYGDGGDKYQSAAELIFTRPQVAETPLDLSGYEAALVKAQKLTDKDNQEEVASVQA
                     SMKYATDNHLLTERMMEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASEQGKTPDY
                     KQEIARPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(30249..32231)
                     /locus_tag="VW343_001155"
     CDS             complement(30249..32231)
                     /locus_tag="VW343_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9755712.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDGYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVMAPVNEEFLNSKGDDFAKATDPNSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         9328 a         7321 c         5758 g         9962 t
ORIGIN      
        1 aaaccagata aggaccaaga gcttaaagct gaaattcaat ccatttttat cgaacacaag
       61 ggaaattata cttatcgccg ggttcattta gaactaagaa atcgtggtta tctggtaaat
      121 cataaaagag ttcaaggctt gatgaaagta ctcaatttac aagctaaaat gcgacagaaa
      181 cgaaaataaa ggagacgttg gtaagaaggc agagaatctc attcaaggcc aatttgaagg
      241 ctctaaaaca atggaaaagt gctacacaga tgtgacagaa tttgccattc cagcaagtac
      301 tcaaaagctt tacttatcac cagttttaga tggctttaac agtgaaatta tcgcctataa
      361 tctttcaact tcacccaact tagaacaagt acaaacaatg ttggaacagg cattcacaga
      421 gaagcactat ggtggagtcc ttctttggga ttctgaaatc ggaaatgttt tatggctatg
      481 agaaaacatt taaatcactt aatcaattgg aacaagctat tatagactat attgattatt
      541 acaacaacaa gagaattaag gtaaaactaa aaggacttag tcctgtgcag tacagaacta
      601 aatcctttgg ataaattatt tgtctaactt ttggggggca gtacagaaaa accttgatac
      661 tatgcgtttt ttgattgtga agacttttat ccacctatta tacaaagtta agtatccatc
      721 acagtttaca attagccttc attacttaac ccattcacca ttatagttta agtagtaacc
      781 acctactgtg gtattaacag ctaaagcacc tgaatcatag gcatagtacc agttaccatt
      841 gacttggaac cagcctgttt tcatatctcc attacttgca tttagatagt accaagtcga
      901 accttcctga taccaaccag ttgccatagc tcctgatgaa cggagatagt accacttgtt
      961 accaaggtat tgccagccag ttctcatatc accattttca gcatctagat agtaccaagt
     1021 tgagccttcc ttataccagc cagttgccat agctcctgac gaatggaggt agtaccatct
     1081 attacctaga aattgccaac cagtttgcat aatgccagtt gttggatcta gatagtacca
     1141 tggagctgct tttagcttct cttcatcata ggtaagaggg gaatccttaa cccaaccacg
     1201 tgctagctct ccaaccgcca atctgttgat gcgcgaatca aagccaccat ccttctgtaa
     1261 ataataccaa tgaccctctt cgtaaatcca accagttttt aaagtatgat aactacgctg
     1321 atcttcaaaa taatagactc atttctctgc tgggctatca tattcttccc catgatgttt
     1381 gttggtattc gtagcagttt ttgcttctaa aacttgcttg ccaacaaatt cttgtaatac
     1441 accatcttga ccaaaataaa accaatctgg tctaagagaa atctctattc ttggagaagg
     1501 accaatcgta acccccttgt gtggagcagg tatatattgc cagccgacaa ccatctctcc
     1561 ggataaagga tcaaaataat agtacttccc atctatcact ctccagtagg tttcttttag
     1621 atcccccttt ttgtagtagg ttctaccatt ttcttggaca aactgccatc cttcagaatc
     1681 atctgcaaat actgtactgg tccctagcaa accgaagaaa aatgtggcta gtgcgacttg
     1741 catcattttt ttcaaaagct tcattttata tatcctccaa cattaaatcc actcaccaga
     1801 tgaagcgaag ttataaactt taccatcgat agtttggcta cctgtaacca ttgctccatt
     1861 tgaattgaga taataccatt ttccatttac tttttgccaa tctgtaagca tcttaccgga
     1921 actgtctaaa taataccaag tatcatttat ctcctgccaa cccgtttgtt tatcatgttg
     1981 tctataatgg taccaactac catttatttt cttccatcct tcaagagagt aacctttaag
     2041 aaccgaataa ataaatggca aatttcgttc atttaattta actgcactgt taatttgatt
     2101 agctccatct cctaaagtat gcactcctac tacacggtga ctagcatcat aaactgtaga
     2161 tccactagac ccctctaaag tatcaacttg gtaatccaag aacatgccat catcacttaa
     2221 aacttgtttt ttatctgtat acatttgatg aattttaaaa ttatatgatg gatagcctgt
     2281 gatagtcaca gttattcctg tcaaattttt ttgactagta ggaagaccca aagtccctaa
     2341 ttttgcacca atgggctctt ctagaattaa taaagccaag tcatattccc ttgcatcctt
     2401 agaatttaaa tttctaaatt ccttcaaata acgaacttcc tttactttga tctttccaaa
     2461 tagttcttga cttggactaa cagccggaag aacataaata tcatccgctt ctttcccata
     2521 gtcatgtctg tagtagttgt gagccgctgt aattaacaca ttatctttaa taaaattcgc
     2581 acttcccctc ccttctacat tcattccatc ggctactcca taatacttag ataaaaccat
     2641 agctgaagtc gaatacggat actgtaaagt attatcaatt ttaatcaaat catcattacc
     2701 gataatactt ctgattgctt ttggtagtat gaaccatacg ttggtgaaat ctcagataat
     2761 gaagaatcat tagatcctgg acctttttct agtgtctcac ttacctcata ttcttcaccc
     2821 gtactagaaa taacactcaa agcagatact gtcgataact ggctagccaa taaagtactc
     2881 gcaataattg aaatacccaa ttttttataa acagttttct tcattattgt agcctcctaa
     2941 tgtaattata gcctactatt ctaaatttct taatctacta tagaatcaag aaatctacca
     3001 ccttctttaa ataccctcca ttatcacata aacaggtaaa cttttcaatt aatgactgcg
     3061 cttttcaatc acgctagagg tacttgcttg cttctttgat actaagttca gccattcttt
     3121 ccttgttttc atcaataaag catgttaccc aagttggatt cgttttggag tagtttcgca
     3181 gaatccagcc aatggcttta ttgataaaaa attctgtttg gttcaagtta tgaagtagaa
     3241 tcttttccat taattgagta ttggtcttct cttttcttaa caactggtgg tcaatagcga
     3301 cacgtctcag ccagatatta tctgataggc tccattttat actcaatgaa aatcaaagag
     3361 caaactagga agttagccgc agattgctca aaacactgtt ttgaggttgc agatagagct
     3421 gacgtggttt gaagagattt tcgaagagta ttaagattat ttcttctagt tcagggtgtt
     3481 catacaccaa actccctact actcgatcta ggatatctac cgtgtcccac caggattttg
     3541 tcacgactaa ctgctctagc ttaggcaaat cggtttcctt tagataagac tgcattgctt
     3601 tcaaatagtt agcagccaca tattggtatt ttctaggctc cttttcccag caagtgtctg
     3661 caaaatccca atcgataatc tttgtttttt tcgcttctgg aaaatatttt tatagagttt
     3721 atttctttca ggcaccgcaa tacctagaaa agaaaattga tggcgcatat aggcttccat
     3781 ggaccttgct tttttagagt cttttgctgc ttctagctcc tcaagtaaat ctgctaaact
     3841 catctaaaac tcctcttgcc ccaccaaatg gtgctgaaag gcatagacag ccgcctgggt
     3901 acgatcgctg acttcaagtt tggcaagaat attggacacg tgggtcttga ccgtcttgag
     3961 agagataaag aggtcatctg cgatgcgctg attttcgtag cccttggcga tgagttggag
     4021 aacatctcgc tcacgcgcag tcaattcttc atgaagttcc atatgattgc ggtggtattc
     4081 aaccttcttg ctaacctctt gctcaatggc cagctcgcca gcagctacct tactgacggc
     4141 atgaagcaat tcatctgcac tagaagtctt gagcatatag cctttggcac cagcatctaa
     4201 gactggcatg attttttcat tgtccaaata agaggtcaca atcaaaatct tggcttcagg
     4261 ccattcttta aggattgcta aggtcgcgtc aatcccattc atctcaggca tgacaatatc
     4321 catgacaatg acatctggac gcagttccaa ggccaagtca atcccttgag acccgttgga
     4381 cgcctcaccc acaacttcta catcgtcttg gaggtcaaag tagcttttca aacccaaacg
     4441 gaccatttca tggtcatcta ctagtaaaat tttcatcttt actcctttat cattccttat
     4501 ctaacagggg aatacggata tcaactgcca gcccttgctt gggagctgtt aataactgaa
     4561 ccgtccctgc catatcttca acccgctcct tgatatttcg cagtccataa ctcaagtcgt
     4621 ctaagctccc taactggaaa ccaatcccat tgtccaccac cttcagttgc aattcaacat
     4681 ctgtctgata gaggtagaca tctaggcaag atgcctgggc atggcggagg gtattgctaa
     4741 tcaactcttg caggatacgg aagatatgct cctcgatttt cttaggcaat ttcgtcatat
     4801 tctgcttgag actaacccta agatcactct tgtcctcaag ctcttttaag agaatttgaa
     4861 tcccttctat caagctcttc tgctccagtt caactggtcg caaatgcaag agcaaaaccc
     4921 gcaaatcctt ctgggctgtt tctaaaatag ctgtgacact ctgcaactgg gtctgcatct
     4981 tttctctatc caatttcaaa gcctgctgac tgatacccga taaaatcatg tgggccgcaa
     5041 acaactcctg actgactgta tcgtgcaaat cccgagcaat tcgcttccgt tccttctcga
     5101 tgatttcctc ttcctgagca aggctatgat tttcagcttt ttgaagagcc tctgtcaaaa
     5161 ggttaagttt acctgataag gacttgaaac tggcatccaa atcttgatct acaacctgaa
     5221 ctacttcttg ccctgccaat aaacgcttga gattagcctg catttttctt agagaaagct
     5281 cttcgatccc tcgccaaaac agggctaaga gacaggtcat ggacatgctg aaaaccaaca
     5341 ataaaaagac aaatttttct gttttttcga catcgtgcaa aaagatagac cagtcaaaat
     5401 caagtatttc cagcaagctg tgggagaaaa aaaagacaaa taggaaggag gtgagagcaa
     5461 taatgacata ggcttgtttt ttcatcctct aaccacctcc acatcaccaa tcatagtggt
     5521 caagaaaatc ttgacactct tgttactctt gagatagtct tttgtttctt gatgatagtg
     5581 ttcattgcgg agggctcgct tgggctggtt gaaaaaagtc aaatccccat agagacagtt
     5641 aacgctgaga ctgacttcca catctacagg tacgatgatt ttggtcgttc ctaccatctt
     5701 tctgaggata atgacattgt catgattggt taagatgacc ctctccagat gaatagtgtc
     5761 cttacccatg aagcgaaaga gattgatatc atcgaattgg caagtctggt agcttgaaaa
     5821 atgatgaaga tttccaaacc aacgattttt ctccttctta accgtcacga cctcttcaaa
     5881 aaccaaattg gtctgctctt tttcctggtt catcatcggg taaagaagaa agaggctata
     5941 gataaccgca acaaaaatag ctagaatcac aaaaggattg agcataacga tgaaaaagaa
     6001 gagaatggtt gccgctacta aaagaagatt atttccctct ttaccagtgt agtagcgaat
     6061 caaaagcaaa aagaggaata gtatcagcag aaaacgcgaa aaatgctctg ataccatcaa
     6121 aatcagagct cctgtcagaa gacaggcttc gataaataaa aagattttaa attttctcat
     6181 aggttcatcc tctcccttct attttatcac aattcaaaaa agtcacctca gtctgaggat
     6241 ggaaaaaaag gcgctggtta cgcctttttc atctgatcct ttgcttcttt taattttcca
     6301 taaagaagat agtctacttt ttgtagatct gctatggtgg cacagttaag ggaacacata
     6361 atcaagcgta gatctgcttt ccagccttgg acaatgccaa tcacttcttc aactgtgtag
     6421 gtttcaacca attccagaac ggttcgtgac aatcccacag ccttagcacc aaaaaccaag
     6481 cacttaatca tatccagcgg attccgaacc cctccactaa ccaagagttc gaccttatct
     6541 ttccattctt gggcattgag aagggcctgc atggtagact gaccccattg attgaggtaa
     6601 tcacgctggc cactacgacg gttttcgata taggcaaagc tggtgccacc acgacccgat
     6661 aggtccactg tacgaacacc gaattcatag gctctttcga ttgtcttggc atccattcca
     6721 aagcccactt ccttgaggac aataggaacg ggaatttgct tgctataatc tgctagatgc
     6781 gattgccagc ttctaaactt cctttctccc tcgggcatga gtaattcctg catgacattg
     6841 acatgcactt gcaatagaac aggattcatc tcttctacag tctgaagtcc taactcgaca
     6901 ggtttgtcca atccaatatt ggttccaagg aggagattgg gatgactaga cttgacagaa
     6961 aaagaatcat ccgttggatt tttgagggct gcgctataag aacccgttac aaataaaata
     7021 ccacaggatt ccgccacctg agccagcttt tgattgattt ctcttccctt attacttcca
     7081 ccagtcatgg cattgatata aaaaggaaag tcccactttc gaccagcaaa ctctgtcgaa
     7141 agatcgattt catccagatt gtaaagaggc aaggaagaat gaatcagctc cacctcatca
     7201 aagctattat aggaactttt ctgctcaagg gcatagagga tatgctcgtc cttacgattt
     7261 gtcgtcatgt cctatccttt cttgatataa gagctcaatc cccagatcgg cccaacgatt
     7321 ttttaaggtt ttggttgatt ccgcatcaaa actcagggcg atgccacagt caccaccacc
     7381 agcaccacta ctcttggcaa cggcctgcaa atcttgactg gcttctttca actgtctaag
     7441 caaaggcgtg taaatatctg tactcaagcc ttctaaaagc ttgctggcta cttctacttg
     7501 ctcgataact ttttcggctt tcccctgttc caaggcttcg accaaagaaa ccaccgtttc
     7561 ttttgaggaa cttaaaaaat tttgattgat attttgcttg atttgctgga ccatgtgact
     7621 cgatacagcc acttccttgg tccatcccac taagaaatca cattctaaag ttggtttcac
     7681 ttgtgaaatt gaaaagcccc aatcacgctc cagaactgtc gccaagtttt cttcttctaa
     7741 ccaagcagcc accttctggc gatcaaatga ctggtagaga accaaatcct ctgccacaat
     7801 acaggcaagg tcgcccatgg aaccattgtc tcctcgctta agcaagacag cgctagtcag
     7861 cttgaacaag agctcctgat caacagaaac atcatacaga gccagtaaag ccttgacaac
     7921 caagacaacg acgctgccac tagaacctag accaaacttt ttcccttctc gttccatttt
     7981 gccacagatt tctagagaaa aaggtcttaa attctgacca cgaacagcga ggaagtctcc
     8041 catcaaagca atcgtttctt gaatcaagct atagtcagga ttaggcctta agtccactgc
     8101 gaaatcaaac atatctgaat agatacggta gctgtcagaa aaagcaatct cagccctcat
     8161 atagatggga atatccttta tcaaagctaa ctgccctggc tctaaaatag catattcacc
     8221 tgcccaatag agttttccgc aagttttaac agcaatcatc ttgactcaaa tcctttgttt
     8281 ttgacacaat caagcgataa cgatgaccga aaatttctga taaatgctcc aagtctttct
     8341 cctgacagaa gaccttaaca ttgggaccag catccatggt aaagtagcag gcctctcctt
     8401 tctcacgaag ctggcgaaca aagtccatag cctcataaga ggcatccgtc agataagaaa
     8461 aggctggact agcagtcttt gtcgtagcat gcatagccag ggcatttttc tccgttaatt
     8521 ctccaatctt ggcaaaatca ttttccttga gataaatcag catatcctga tagtccttct
     8581 cagactgacg aacccagtcg tcgaaagtcg tcgaggtttc cacacaaagt ttcatcccgt
     8641 cacggctaga gattggtttt ttcttgtcct ctagcaccaa cataatcata gctagtttca
     8701 agtctgtctc tacagggtaa atttctccac tatccttatc ccaggctcct agtggtccat
     8761 aaaaactccg agaagaagaa cctgaggcaa atttggcttc ctgtgccaac tgacttctat
     8821 ccaatccaag cttgaaataa gcattacaag ccttgaccag ggcggacaaa ccactagaac
     8881 ttgaggacag acccgctgcc gtaggcatat tgttttgagt atcgatacgg acaaagccct
     8941 caccagctgg acgataacgg tcaataatct tactcatctt ggcatgctcg acctcatttt
     9001 gtagctgacc attgatgtaa aattcgtcag ctgttacatt ggctggtaaa ggcgacaagg
     9061 tcgtctctgt atacatattt tccaaagtta gagaaatact gctagtagca ggcaccatct
     9121 ctttttcttt tttctttccc caatatttga taatagcaat atttgcgtag gaacgtactg
     9181 ttacaggctc tctatccatg tctgaacagc tcctttctct tctaatcttt ctgctagttc
     9241 ttgtgcgtgt gtcaaattgg ttaccaaggc tatgatacaa cctcctagcc caccaccgct
     9301 catcttggca cccagagcac catggctaag agtcgtttca accaaaaagt ctgcctcagg
     9361 gctactgact ccaatttctt ttaaatgtaa atgcgcttga ctgaggattt gtcccagtcc
     9421 ttcagcatct ttttgtgaaa tcgcaacttc tgcttgctgg gttaattctc ccaaggcatg
     9481 caaaaacggt agggcatcct tgcccttatt ttgaaccact tggatggctt cacgagtatg
     9541 accataaaca cccgtatcgg caatcaccaa ataggcggat aaatccatct caagttctgt
     9601 aaatcctacg ttcttgataa agcgaatagg ttggtcacta agacaggtct tagcatccaa
     9661 accactagga ttcatatggg caatcatttc agctcgattg accaagattt ctagtacatc
     9721 atgaggcaga tcagcctgat agtagtcaaa tactgcacga atggccgcta tgctgatagc
     9781 cgctgacgaa cccatccccc gtttctcagg gatagccgag tcaatctcac aacgaatgca
     9841 ggcttctgtg atattcaaat actccagtga ggcataaacc gccatggaca aggtatcctc
     9901 ctcataaagg cgccaaggac tctctgcagg aactacctta caggtcacct ccacctccaa
     9961 aagaggcagg gaaatggcag gataaccgta aacgaccgca tgttccccta ttaaaattat
    10021 cttactatgt gcctgaccga caccaacttt ttttgtcatt ttttcctttt actagacgaa
    10081 aaaacgtctt atttttcata caagtattaa ttctttccta tctattttat tatattttca
    10141 caaaaaaagc gattgtttcc attcacaatc gcttctttca ttattgaacc cattcgccat
    10201 tatagttgac agaatagcca tctacggtcg tattcactgc caaggcacct gagcgctata
    10261 agcgtagtac catctgccat tgacctggaa ccaacctgtc gtcatagaac gacgaaagaa
    10321 actccatacc attaagtaaa gaggaaagtc gtgagggagc atgcgccatt gacaacctgt
    10381 tttagtgacg tacaaagtct cattaacaag tactcgtttc ggccatttat aggtgcggtg
    10441 tttggagaaa tagggttcaa tcttcgccca ttcttgatcg tttaaatcag tatcatatgc
    10501 tttgcgtatc ataactctag cttaacattt ttttgtgaat acaggttcta aataatcgac
    10561 cacgaaaatt tcttaagtgg aaaacgcctt atgaagtatg ctacgggaaa cttatgcact
    10621 taatttgaca attcaagatg taaaaatata tactatagta gattgaaact agaatagtac
    10681 acctctactt ctaaaacatt tttagaaatc gatttgactg tcctgatcga tttgtcctgt
    10741 tattatttca ttttactata atatttgata agttatccta aaagtgttat tatgttgttg
    10801 tgttatagat tgattgaatc tacctaaagg atcctattca attactagaa ctatcacata
    10861 ctcaaggtca gctcacagat gagcaactat tttggttaca atgtctacta aatttaagtc
    10921 aaacaaataa tttagtcaaa attaaaaaaa tagaggaaca taaatatgat tacaaaacag
    10981 aatgtaatag tgttctacaa tttttactag ataaaactgt aaattctgaa ggaaggatca
    11041 cttcttcaac agaatttaga aatttcgtaa gtaatttatc attccaacac ggaatagctg
    11101 gactactgtt tcctctaaat aaattgtacc ccccagaact ggattctaaa atactctcta
    11161 tcatcaagaa ggcagtgaca attagaacga cacatacata tgaatatcaa tactcactgc
    11221 tatttggtga tgcaggctat ctatggttac tcctacattt attttctatc agtaaaaatc
    11281 aatactatct acaattagca aacgtcaccg ctaagaaatt aatagagaat tatgatactc
    11341 tagaggaaat agactttgca ttgggaaaat ctggtgtcct attatcatta ataaaatact
    11401 atcaatttac caatgacaat actcttaaaa ctttcatcca caatagtata tggggaaatt
    11461 tatcattatt tcctacaaag agatacagcc aaagaaagca ttttagacta tagctttgct
    11521 catggatatt gtggaattgc atatgcttta tttgcctatt ctaaagtctt agaaccttct
    11581 atgttctata atgatctcca tacattccat gctgaattaa aaaaattatt agaaaaagtt
    11641 acttctaata ctgaaaattt aggaaattta caactttctt ggtgcgaagg aatttccgga
    11701 ataatcttat atctttgtat gtacgattgt gacggaaaca aagatattat tagtaaatat
    11761 caagaatttg tttttaacca tcatctaaaa atgatgacag gatattgcca cggaataact
    11821 agcttactac aaaccactgt ctacaatcaa aacaaattac tgatgaaaaa atccaacagg
    11881 taattttagc atgttctgaa cgagatgatc acggtttact gatgtttcaa ggagatagtg
    11941 gtaaagcaga tttgtttgac ttcggaatag gaagcagggg gtatattggt gtctattaaa
    12001 taataaattc ccattcgatg tgcagacata aggagaaaag tatgaaatta ttttggacaa
    12061 acaacatata tagacagttg ctgctaaaca gctgtttttc atcattcggc gacagtattt
    12121 tctacctcgc cattatcaat tatgtggctc agtacaattt cgctccgcta gcgattttac
    12181 tgatttccat ttcagagata gttcccctac tatcgcaact ctttctcggg attctaggag
    12241 attttcaaga aaatagagtc aaacacgcac tctgtattgc caaaatcaaa atcctgctct
    12301 acgctatttt gacactattt ctcgtcttgt cgcccttttc attaatttca gtcattatga
    12361 ttgtcatcat caacctcatc tctgacacct tgagctacct gtctgcctac atgatgaacg
    12421 ccctctacat cagtgtaatt aaggacgacc tgcatgatgc catggggttc aggcagtctc
    12481 tgatgagggt tgtccgcatt gtcgccaatc tagctggcgc attccttatc aatgttatga
    12541 gtattcaaac tatttccctt atcaacactc tgacttttgt cattgccttt ttgggcctgt
    12601 atgttattcg acataccttg tatgaggttg aaaaaaggat tgaaatgtca catacagcac
    12661 tgagttttaa gaaatatttt caacatctta aacagtcgct ggctgtgctc ctgaggttaa
    12721 aagataccgt catactactg tttctgacga ccagtatgat tgccatcttg gatgtgtccc
    12781 ctcggctgat tgccctccgc ttcatccaac agacactagc acaactgagc attgggcaac
    12841 tcttcgccct gctctccatc atcatgtctt gtggagctat ccttggcaat atgaccagca
    12901 gtaatctatt taaaaatatc cgtttcacgc acctcttggt tttctgtgag atttccctat
    12961 tggctctaat aactagtatc ctttgtcaag cctatatcgt aattttcgtg accagtttca
    13021 tcagttctac gattatcggc attctcagcc ctcgcctaca agcagctgtc tttgcccata
    13081 tccccagtga caagatgggg acggtgggct ctgctctgag cacagtagac attctcgccc
    13141 cgtccctgct ctccctatta gccctatcca tagcatcggg cgtttcggtg cagttagcat
    13201 tgatattttt gtatcttatt ttgattgctc ttatcttttg tcaatggtta gtcaagttca
    13261 acactcataa ctaacgaaaa agcatgtgta gatttcacat gcttttaatc tccccaatcg
    13321 tcaggtcaag tacaacaaag tcacttcttt gattaagcga gtgttctaat ataattataa
    13381 gcgccctgtc attaccgaac ccattcgcca ttatagttga cagaatagcc atctacggtc
    13441 gtattcactg ccaaagcacc tgagctataa gcatagtacc agttgccatt gacctggaac
    13501 caacctgtct tcatgtctcc attacctgca tttaggtagt accaagttga accatcttga
    13561 taccaaccag ttgccatagc tcctgatgaa cggagatagt accatttgtt cccaaggttt
    13621 tgccaacctg ttttcatatc gccatttggg tggtctaaat aataccaagt ggtaccttcc
    13681 tgataccagc cagtggccat tgctcctgag gaacggaggt agtaccactt attacctaga
    13741 tattgccaac ctgtttgcat aataccagtt gctggatcta ggtagtacca agctgaagca
    13801 tcatttatcc aacctgcacg tctttcacca ccaatatagt tttctccatt aatttctgtt
    13861 ttagctagat aataccagtt agactgatca taaagccaac ctgtctctaa agaatgattt
    13921 tgattaaagt aatagttcgt gtaataacgc ttctcttctt tatcttctga attttcacgt
    13981 ttttccccat actttcttcc aacactgtct tgagttttaa tctctaattc tttccaacca
    14041 acaaactctt gtagcactcc atttttatca aagtagtacc actctgactt tggaaaacct
    14101 tctaatctga taccatttgg gtaaggacca attgtactac ctttagatgg aaacgggata
    14161 tattgccagc cgacaaccat ctctccagat agagaatcaa aataatagta cttaccatca
    14221 atcactcgcc agtaggtttc tttgaggtcc ccctttttgt aataggttct accgttttct
    14281 tggacaaact gccatccttc agagttatct gcaaatactg tattcgtagc tagcaaacca
    14341 aagaaaatta ctgttagtcc agcttgcata gtttttttca aaagcttcat tttatatatc
    14401 ctccaacatt aaatccactc accagatgaa gcgaagttat aaactttacc atcgatagtt
    14461 tgactacctg taaccattgc tccatttgaa ttgagataat accattttcc atttactttt
    14521 gtccaaccta ttttcatatc tccattagaa ggatctaaat agtaccaagt tgaaccatct
    14581 tgataccaac cagttgccat agctcctgag gaacggagat agtaccattt gttcccaagg
    14641 ttttgccaac ctgttttcat atcgccattt ggctggtcta aataatacca agtggtacct
    14701 tcctgatacc agccagtggc cattgctcct gaggaacgga ggtagtacca tggagcaggt
    14761 ttgcttctat caatgtcaat agtaacatga aaatcttgag tccaaccctt agcaacttca
    14821 ccaattggta gtggattgta agaatcatca ccaaaatttc ctagcttatt taaataatac
    14881 caattgccgt cttcataaat ccagcctgtc tttacagcat aattattatc aaaataataa
    14941 gttctctttt cagcagatag aggatattgt tcaccgtata cttttcctgt attttcagac
    15001 gtttttgcct ctagtacttg tttatctgtt tgttctagca aagcaccatc ttgtccaaaa
    15061 tagtaccact tctcctgaag gccaatttcg aagactggac gattatcaaa taaatcatca
    15121 cgataacctg ttccaggaat ttctaagtat tgccaaccga caaccatctc tccaatatca
    15181 ccaaaatagt aagttttacc gtctatttta tgccaataga ttgctttatg atcatctttt
    15241 acataatatt ttctattatc cttatcaaca aatcggccac ctgtggtatt cgcaaatacc
    15301 gtatttgtag ctagcaaacc aaagaaaaag actgctagta gaacttgcat catttttttc
    15361 aaaagtttca tttatatatc ctcctaaaac gattgtagca aagacacgac tgcatgtcaa
    15421 tatataaaaa ctcctcaaaa aagcagttac acaactgcaa ctgcttttct attcttgcat
    15481 ggtgtaacca acaccacgca cggttttaat gtagcttttt tgtcctttaa catcaagctt
    15541 gctccgtaga tagcggatat agacatctac gatatttgtc tcggtcgcac tttcatactt
    15601 ccaaacactt tccaacaatt gctcacgagt caatactttc ttgcttccca taagtgtctc
    15661 caaaaggtca tactcacggc gggtcagagc aatcatctct tcaccacgat aaaccgtgtg
    15721 atgttcaaca tctatcctaa gattgcggta ggtccttgga actttcatca gactgcagtg
    15781 ttgatcaatg aagtcccgac ctcggaagat cgccgaaata cgcgctacca gattttcgat
    15841 aaagactggc ttatagatgt atgaaactgc aaaacgctga acaacttcca gctcttcttg
    15901 caagtcttcc caatgatcta aaatcatgat gactgaggca ggtttagttc gactcaattt
    15961 ttctgcaaaa tcctgagcca tcatatctcc cagattaacg ttcaataaaa tcaaatcata
    16021 gtctgtctga agagccatgg aaagggcttt ttgcccctcc tctaccagat caacccgata
    16081 ctgctctttc tggagttcca aacttaaaaa atgagctaga tttcgttctt tctcaagtaa
    16141 taaaatccgt ttccccatgg ctgacctact tatttttcgt cataccaaga gtagtggaag
    16201 gttccttctt tgtctttacg ttggtaagtg tgagcaccaa agtagtcacg ttgtgcttgg
    16261 atcaagttag ccggaaggtc agctgaacgg tagctatcaa agtaagtaat agctgctgag
    16321 aaagttggca ctggcacacc agcttgaacc gcaagagcta cgatatcacg cactgcttgt
    16381 tgatacttag cagtaacatc caagaagtac tcgtccaaaa gaaggttggc aagatctgca
    16441 tcgcggttgt aagcatctgt aatcttttgc aagaaacgag aacggatgat acagccatca
    16501 cgccagatag atgcgatatc tgcaaatggc aagttccagt tgttttcttt agaggctaca
    16561 cgcaattgag caaatccttg tgcgtatgaa atgatttttg agaagtaaag ggcttgacgg
    16621 attttttcaa tcaattcagc cttgtctcct tcaaagttga aggcagctgg ttttggaagc
    16681 accttgctag catgtacacg ttcttctttg taagttgaaa tgtagcgtgc aaacactgac
    16741 tcagtaatca gtgacaatgg tacaccaagg tcaagagatg attggctcgt ccatttacca
    16801 gttcccttgt tacctgcagc atcaaggatg tagtctacga ttggtccatc ttggtcttca
    16861 tcgtctttac ggctcaagat atcagctgtg atttcgatca agtagctatc caattcaccc
    16921 ttgttccact cagtaaagat ttcagccata tcctctgcag aaaggcctag caagtgttgc
    16981 atcaagtcat agctttctgc gatcaattgc atatcaccgt actcgatacc gttgtgaacc
    17041 attttcacat agtgaccagc tccatcagga ccgatataag tcacacatgg tttgccatct
    17101 tctggtgctt tagctgagat ttcttcaaga acatccgaaa ccaattcgta ggcctctttt
    17161 tgtccaccag gcatgataga aggaccttca agggcacctt tttcaccacc agaaactcca
    17221 gtaccgataa agttgatacc tgagtttgcc aattcttcat tacgacggat ggtatctttg
    17281 tagaaagtgt ttcctccgtc aatcaagata tcacctttgt caaggtgtgg aagaagggct
    17341 tggatagtag catctgtacc aggtccagct tgaaccatca gcatgatacg acgaggtttt
    17401 tcgattgagt ttacaaaact ttcaacgtca tagcttggta caaaattctt ttcaggatgg
    17461 caagcaatca catcttccgt tttttcttta ctacggttgt agatagcaac tgtgtaacca
    17521 cgtgattcaa tattaagggc aaggttacga cccattacgg ccatacctac gacaccaaag
    17581 ttagcttttg tcatttgaca ctcctcttgt ttcatttgtt ttattttatc atttttactt
    17641 ttgaaaagaa agaattttgt aacgactgct tagtagtcca agtcatccgc atgaccagcg
    17701 ccatttccga caaagtagcc tgtcttacag ttaagggtaa agagataggt tccatctggc
    17761 ttatagaggt tgtaataacc attgccgtta acgatattga cacgttcaag gatatattgg
    17821 tctccagtga tatagccacg tgaacgtgaa gttgctagaa tttgttccaa aacaccatcc
    17881 gcaaaatccc aggcagagtt attactatca tcaatagcag actgattata ggcaacacga
    17941 ctggcacttc tttgaacagc aacccctgca ctcgagagac ccatattgac ttcactgcga
    18001 ctacttctag tctcatttga actacttggt tttggctcac tagtcgtatt tgaacttgct
    18061 tgacttgaac ttgagctgct agtttgactt gaacttgagc tagaagtact tgtttgctgg
    18121 ctcttaccaa gactgatagc cttatctagc actttatcaa gctccgtatt tccagtttta
    18181 atatccgtaa atttagcatc cgatttggct ttggcattgg tatccaacac accatccaca
    18241 atagctggtt tttcaaattg tgcattgaca tcttgaatgg ccttgatttg cgttactaga
    18301 ctatcatatt tagatttggc aagcgtatgt tcacgactac cttccagctt atcaagtaaa
    18361 gtcttgagtt gactcagttt atcaaactgg ctatttttca aagccgtttt attgctattt
    18421 gtgtaaaagg cgtcataaag tgtattaaaa tcatccacat ccgattgatt ggctgtagtt
    18481 gattgagaag tttcaatttc cttggtcgaa cgagccactt gacgatagac ataataagca
    18541 ctgacacaaa taattactga tacaagcgcc aaaacggtca aaataaagcc ttttttactt
    18601 ttcttttctg agtcctcttg atccagacga gaaaggtttt gctcctcttc cacttcttca
    18661 gtcatttctg ttggattttc atcatctagc aatagaggat ccaagccttg ctcctcatcg
    18721 tccagaggca agggtagtaa gatgtcttca gaagatggaa cttcctccga agaagcttct
    18781 gacttctcta cagcctcacg catctcttga attaaatcat ccagactctg agtttcaacg
    18841 aactcctctt ttttgtattg acgagtcgca aacttatccg cctcaatttc atctctgtgt
    18901 tgcttaacat acttgtccaa aatggaatcc tcaggcaaga ctcctgcttc cacttcttca
    18961 tttttacgaa tagcttgacc aactgttagc tcttttgctt catcaaaatc aaattgcggt
    19021 tcttgacctt cttttttatg acgatttcgt ctttttttac tcatgagtat ccctttctat
    19081 tttacctctt gccgtttgac acgctgacca aaatattgat atagatccac tttcaaggtt
    19141 ccgttgtata acttacgctt cttatctgct tggctaccat acttgctttc aaaagcttca
    19201 tcactagtca ggataaattt gctccaagtt ttcagcggtg caaatacttg ccccatctca
    19261 gcatagagct tggtcactcc tgcatcatct gacaaacgtt caccataagg cggattggaa
    19321 atgattactc cattgatttt atcggaacgt aaatcctgca cgcgcatctg cttaaaagta
    19381 atatctcctg caacaccagc tgcctgagca ttggccttag caatttccac catgcgagca
    19441 tcaatatcac agcccatgat atccaactca agcttacgtt ctactttttt agccgcttct
    19501 gtgcgcactt cttgaatcaa gcgatcgctg atccagttcc attcatcaaa tgcaaaagag
    19561 cgacgaagac ctggcgccat ctttctagca atcataactg cctcaataca gaaagtcccc
    19621 gaaccacagg tcggatcaat caaaggcttg tctggatacc agttagaaag ttgtaaaatg
    19681 gctgctgcca tattttcctt gataggagcg ccaccttttt cggtacgata accacgttta
    19741 aagaggctag acccggtcgt atcaatcatg acagttgcca catctttgag aatagagacc
    19801 tcaatcttaa actctgggcc attctccatc agaggaaccc cttctgggcg agcatagtgt
    19861 ttctgcaatt tcttgacaac agctttctta gaaatagcct gaacactggg ctcattgtga
    19921 agtttggact taacacattt agcttttgaa atcggtaacc gagctccaag tggtaaataa
    19981 ttttcccaat ctaaagcgaa aactccctga aatagctctt caaaagtctt agctgggaac
    20041 gttcctacga taattttgat acgatctgct gcccgaagcc aaaggttggt ttcgataata
    20101 gctctcacgt ctccttgaaa acgaacacgt ccattttcaa cctgacaatc gtagcccaac
    20161 tctcgcactt cacgaccaac gacagcctca agccctgctg ccacagttgc aattaaatta
    20221 aattcttttt tcatgttaca gtcttgcaag acctttctat atgtccactt taaaaaatga
    20281 ggttgagaaa atttctcagc cccaacctat atgcaatttt ctataagcca tgttttgttc
    20341 cagaagtgac taggaccact tccttcgata atcatctgtc taccactaac agctctagct
    20401 gatagcagtc agaatactac gttcgtttcc gcatactcca tgccccgacc aaaatttggg
    20461 ttgctagctt gaggggttta ccgcgttcca ctctctctgt ttccagaaag actccgtcac
    20521 tgtggcactt tcaagcctac tctggcctat ccaaggactt agccatttca actgccgtaa
    20581 cgatttctcg tccctaggct tatggtttcg cctagcacaa acactacagg catcacagcc
    20641 tgtgctagca tggactttcc tcatgagaag caattctcct cacgcgatta tccaaaaatt
    20701 gcacatctca aataactact taaaaatctg agttatctaa aatttgttta ccaaaaactt
    20761 ctttttccaa tctattcagg cgtttcaaaa tatcaaaatt cgtcatagaa cttgtaattg
    20821 ccgcttcaag gggttctgct tgaactggtg aaggtttcgg tttacgagtt aattcttcct
    20881 tcaaatccgc aatttcctga cgaagtgact tgaccaaggc agcataggtt tcatagtcct
    20941 tgatgacatc gtctaaaaac tcgtcaactt ctactttatt atagccacgg acttcacgtc
    21001 caaactcttg ttcaaaaata tctttcgctg aaaaaataat acttgccatg tctctctcca
    21061 ttctcgcttg ctagtattat tatataaaaa aaggcaaaca aaaatcaagg tcaaagcttc
    21121 aattttcgga aaaattttca gcaagttcat ttaattgatc aaatgttaat ctctttataa
    21181 aatagtcctc ttgatttttc atcttttggt aaaaataagc tagttttgtt tcattttctt
    21241 catcataaaa aagataggaa ctagtcgtgt tttccagcaa aaactgctgg taatctctta
    21301 actgcccctt gtgttcatag cgaggataag catatttgac aaagtctacc tgcttaaagc
    21361 gactcagttt catctgatta ccttcattcc aattttcccc atgggtttca aaagcaaaaa
    21421 tggtggccaa ctggaaaccg tattcggttt tcatctcctg agcaacctct aaaacccaat
    21481 attcaaaacc caaacttcct gtaaacacaa gccaagacac cccatctgct gccatagcct
    21541 ctaaatcttt acgtatcgct tttttaatca atttaagacg aggatcctta tcagaaaata
    21601 aacccaaatc aaaagtagaa tagcctaaaa ccaaagctgt agccattttt aaccctttct
    21661 gtgcaaatta tggtataata aagtgatgtt attgtaccaa taaggagaat tatggtcaac
    21721 tatccacata aagtttcatc acaaaaaaga caaacatctc tttctcaacc caaaaatttc
    21781 gcaaatcgag gaatgtcttt tgaaaagatg atcaatgcta ccaacgacta ctatttgtct
    21841 cagggcttgg ctgttataca taagaaacca actcctattc aaatcgtaca agtggactat
    21901 ccacaacgaa gtcgtgccaa gattgttgaa gcctattttc gacaagcttc aacgacggac
    21961 tattctggcg tttataatgg atattacatc gactttgaag tcaaggaaac aaaacaaaaa
    22021 cgtgcgattc cgatgaaaaa ttttcatcca catcagattc agcatatgga acaagtcctt
    22081 gcccaacaag gaatctgctt tgtccttctt cacttttctt ctcagcaaga aacctactta
    22141 ttgccggcat tcgatttgat tcgcttctat catcaagata agggacaaaa atcaatgcca
    22201 cttgaatata ttcgagaata tggatatgaa atcaaggctg gtgccttccc tcaaattcct
    22261 tatctcaatg ttatcaaaga acatttatta ggtggtaaaa caagatgaac aaaccaacga
    22321 ttctgcgcct aatcaagtat ctgagcatta gcttcttaag cttggttatc gcagccattg
    22381 tcttaggcgg aggagttttt ttctactacg ttagcaaggc tcctagccta tccgagagta
    22441 aactagttgc aacaacttct agtaaaatct acgacaataa aaatcaactc attgctgact
    22501 tgggttctga acgccgcgtc aatgcccaag ctaatgatat tcccacagat ttggttaagg
    22561 caatcgtttc tatcgaagac catcgcttct tcgaccacag ggggattgat accatccgta
    22621 tcctgggagc tttcttgcgc aatctgcaaa gcaattccct ccaaggtgga tcaactctca
    22681 cccaacagtt gattaagttg acttactttt caacttcgac ttccgaccag actatttctc
    22741 gtaaggctca ggaagcttgg ttagcgattc agttagaaca aaaagcaacc aagcaagaaa
    22801 tcttgaccta ctatataaat aaggtctaca tgtctaatgg gaactatgga atgcagacag
    22861 cagctcaaaa ctactatggt aaagacctca ataatttaag tttacctcag ttagccttgc
    22921 tggctggaat gcctcaggca ccaaaccaat atgaccccta ttcacatcca gaagcagccc
    22981 aagaccgccg aaacttggtc ttatctgaaa tgaaaaatca aggctacatc tctgctgaac
    23041 agtatgagaa agcagtcaat acaccaatta ctgatggact acaaagtctc aaatcagcaa
    23101 gtaattaccc tgcttacatg gataattacc tcaaggaagt catcaatcaa gtggaagaag
    23161 aaacaggcta taacctgctc acaactggga tggatgtcta cacaaatgta gaccaagaag
    23221 ctcaaaaaca tctgtgggat atttacaata cagacgaata cgttgcctat ccagacgatg
    23281 aattgcaagt cgcttctacc attgttgatg tttctaacgg taaagtcatt gcccagctag
    23341 gagcacgcca tcagtcaagt aatgtttcct tcggaattaa ccaagcagta gaaacaaacc
    23401 gcgactgggg atcaactatg aaaccgatca cagactatgc tcctgccttg gagtacggtg
    23461 tctacgattc aactgctact atcgttcacg atgagcccta taactaccct gggacaaata
    23521 cccctgttta taactgggat aggggctact ttggcaacat caccttgcaa tacgccctgc
    23581 aacaatcgcg aaacgtccca gccgtggaaa ctctaaacaa ggtcggactc aaccgcgcca
    23641 agactttcct aaatggtctc ggaatcgact acccaagtat tcactactca aatgccattt
    23701 caagtaacac aaccgaatca gacaaaaaat atggagcaag tagtgaaaag atggctgctg
    23761 cttacgctgc ctttgcaaat ggtggaactt actataaacc aatgtatatc cataaagtcg
    23821 tctttagtga tgggagtgaa aaagagttct ctaatgtcgg aactcgtgcc atgaaggaaa
    23881 cgacagccta tatgatgacc gacatgatga aaacagtctt gagttatgga actggacgaa
    23941 atgcctatct tgcttggctc cctcaggctg gtaaaacagg aacctctaac tatacagacg
    24001 aggaaattga aaaccacatc aagacctctc aatttgtagc acctgatgaa ctatttgctg
    24061 gctatacgcg taaatattca atggctgtat ggacaggcta ttctaaccgt ctgacaccac
    24121 ttgtaggcaa tggccttacg gtcgctgcca aagtttaccg ctctatgatg acctacctgt
    24181 ctgaaggaag caatccagag gattggaata taccagaggg gctctacaga aatggagaat
    24241 tcgtatttaa aaatggtgct cgttctacgt ggagctcacc tgctccacaa caacccccat
    24301 caactgaaag ttcaagctca tcatcagata gttcaacttc acagtctagc tcaaccactc
    24361 caagcacaaa taatagtacg actaccaatc ctaacaataa tacgcaacaa tcaaatacaa
    24421 cccctgatca acaaaatcag aatcctcaac cagcacaacc ataacattta tcatccagat
    24481 ttttctgggt gattttttca tagcactatt gtatttatgt gcttaacaaa agcctgagaa
    24541 gatatcttct caggcttttt ctgtactata aacctcattg gaaaagatag agactgcttc
    24601 cgtaatcttg ttaagaggtt ttactaaata ctagtctttc ttcgttttta ctacaaagag
    24661 agcagatagg gctaggctaa cacttgctag gaagagggct gtgtcagatt gactctcacc
    24721 tgttgctggc aagatttgtt caggtgtttc tggtctagct atttcttgct tataatctgg
    24781 cgtcttacct tgctcggaag ctaaagatct aagtttaaac tcaggtttct ctacagttgg
    24841 agcatctggt ttcgtagcag aatcttttaa ttggttgaga taatctgcaa agtattccat
    24901 cattctttcc gtcaagagat ggttatccgt cgcatatttc atgcttgcct gaacgctagc
    24961 tacttcctct tgattgtctt tgtctgttaa tttctgagcc ttaaccaaag ctgcttcata
    25021 gcctgacaag tcaagaggtg tttctgctac ctgtggacga gtaaagataa gttccgctgc
    25081 agattggtat ttatctccac catctccgta tgtcttggta ccagtaagga caattttctt
    25141 agccttgatt gtcttaccaa agtcaatatc ttttggtttg ttgttatttg gccaatcagt
    25201 tgcagtaaag gtatgctcct tgccagactc atctgtcaca acaagtttca catctcgcaa
    25261 gttaccattt gaacctgatc cacgtggaac ataacgaagt ccagtgattt cagttggttc
    25321 tttcaagacc atggttgcag gcttgcctac atctcctccg ccccatgatg tatgccatag
    25381 actagacaca ttgccatcaa aggcatttgc aagaccttct tgagcctgag caggagctgt
    25441 aagacttgca aagtcatctg ctaccaaagc cgttttcttc tgaaccaaag cattcttcaa
    25501 agcttcaatc ttggcaatct ctgcacgcgc ttcttccaca ctgatatcat catcggcctg
    25561 actgaggtta aagaccgcct ctttcaaagc atccatagac tctttggtgt agttagtcat
    25621 ggcaaccgtt ggcaagtagt tcttcagagc attttctgtc aacatcttac ctgttagggt
    25681 aatttcttcg atttgaagat tatccatcat gaagtcgtta taaccacgga agttggcatt
    25741 tccaccagaa tcaccacgag tattacttgc atttccagtt gagtagatac ctacccaagt
    25801 atctcctgtt tctgcacctg tcacgaggaa ggttgccttc ttggctttct tggaatctgt
    25861 ccaagtattt ggcaattcat gcatttctaa gttgcttgct tgagtgccac gacgacctga
    25921 ctggaattct cccttaccga ctacaaaagc ataggtatta tctgaacctg cttcgtattc
    25981 aaaggttaca cggtaggtct taccagcttc aaagcggaag ttttgtggga tggtttggta
    26041 aaccaagtta cgacggctaa ccagtccatt ggtcttgagt gaccaatttc catcgataac
    26101 atcatcaact ttcttaccgt tccaaccacg ttgtgtatat ggatcatgct tttcagacaa
    26161 gtgagtgcgg ttgtcttcaa caccttcgac accacccact acgaatggga agataccctg
    26221 agcaacattt tcaaagtctt gtttgaaggt gcctttacct gtatcatggt tttctccgta
    26281 catgcttgaa ttgttttcaa aggtacgaat ttcatcaaag taagttgctt gatcaccagc
    26341 ttcgcgactt aaagtaagag taacatttga tacgtccgat ccagttgtaa agaaggcgta
    26401 catgttttgg aagtaacttg tattgtcaac tgtagcattg tcacgacgtg tattgtgggc
    26461 gtaggcctta acatagttga gagcgagaga cttattggta taagtagtca cttctttttc
    26521 accagtgttt acagtgatgc tcgccttggc attactacgg ttatcgacac cgacataaac
    26581 ggcatacttg gtatttggtt tcaaaccagt taatttctga gtgagactaa ctttttcttt
    26641 gtttccttga atacgaagca tatcgtttgc cccttgagac ttgacaattt ctgccttaga
    26701 agcatcgcct gaaatggtcc agtgtttcaa ggtaccgcta ttaaatcctt gatcatagat
    26761 gtgcatgcct tcactccatg acatttcagg attggtttgt ttcgaacgat agaaaacgta
    26821 tggttgattt gctagaagat ctagggtaat tttaccatct tttacagtta gttcttgctc
    26881 ttctgtctta ccttggtcag ttagcttgta aaggtaaacc ttgctctttg cccaatcact
    26941 tggaagggtc caagttgttg caccggcctg cgtattgaag tagtacatct tttccttatc
    27001 agtagaaagt ttcttaccat ttgcatccca gttccaagga gtcaagtaag ctgaaccatc
    27061 ttggatgaca cgtccgttga gcgttactgt acgtttgcga tattgtggac tattgacatc
    27121 atttgactta cgagttacaa ctactttatt attgttagca tctaccaatt ccactcgcat
    27181 ttctggagtc catttatagg tgctaccgtt atcggtcata gtcaccggtg taccattttc
    27241 ccatttactt acagtgaagt gttggaagta cttggtcatg acgtcatggg caaataagtt
    27301 agttacatag ccattgtagt cacttcttcc ttgccagcct tcaaagtctt tcatgctgta
    27361 gccacctagc agtggatagt tggctgcacc accataactt ctgtagtccc ctacccaagc
    27421 atctttttgg tggttacgga taaagcgggt gatggcactg ttgatacctt tattggtgta
    27481 gccaccgtag gtcaagtcag ctgcccagtg atggaaggta gagtcgtact caccaccatg
    27541 gccccactcg atcgcaaagc gccagccttg tttgttaatt tctttagcaa gaacgtgggt
    27601 agcccaggca ccgttatcac ctgattgacc attaccccaa acgtccacat agataaagtc
    27661 gagaccgtca ccaagttttt tcttcaaatc ttcccaacgt gccaaacgac catgagctag
    27721 gtcataggca gcatcaatgt tgataccttg atctagccag ttccaaccat agctatagct
    27781 tccatctgga ttcttacgga gaattttttc attgaaatat ttagactcag gataagtttc
    27841 tgaagcgtta acatggatac ctagatgagc tccatatttc ttagccttct caattagggt
    27901 cttgaagtct tcgacaccac cgatacgctt accaatatca gcatagttca agtgaccaga
    27961 gtcatgacct tcactaccat atcctttaag gagaacacct tgcccaagac catctgtgtg
    28021 gagattgatt ttcttgatac catccaaggt cataaggaat ggattttgtg cttgagaacc
    28081 aaagttcatc gcgatacggt aagctgtgat atccttaact ttttcccaac cttgagggtt
    28141 gttcataatg ctacgataag caatggcacc atcctgccaa tcaactttct tgtctgcatt
    28201 ggcatcttca gtgataacaa ccttagcact tggaagttcc ttcgtgtatt ctgggaaaac
    28261 aatgccctta taagcttttt cccattgcca ttcagagctg tggattccta catagttggc
    28321 atttccgact gtttctttat aagctgtcaa acgagtccag tcattcgaac caccaccata
    28381 gctgttttga gagttactcc aaacaccagc agcaagctta tctgtagaaa caaatccata
    28441 catgtaaccc ttagccaaat ccttcattgg attggttaca tcgatatgat catctccgct
    28501 gacatgtgta ttgtttgaca tggttgcccc atcaaactta gcaccagttt gatcactaga
    28561 aacagagact aaagcattgc cgaggaaact aatagaagaa agtagttttc tttcgtcatc
    28621 gatcttttga cctggagtga cttgattgtg gttgacaatc ttggtcacat caaagtgcaa
    28681 ttgattgtcc acaacttgca agcgtactgt catttccgca ttgattaagt gagcatcatc
    28741 gcgaagcttc atcaagtact ctgctgttgt ctcattgatt ttcttataag tgacttcagg
    28801 ggtgattcgg tggttattga taaagacttg gttgaactgt tgcacctgtc ctggcaaagt
    28861 atgtccattc aaggtgtatt ccttgacacg agggaaggct tggtcaatca ctgctttgag
    28921 gaccttagac tgaatcgtgt cataagtcac cttgctatca tcaacttcag gacctgtttc
    28981 tttttcagca ggggtatcct ctgtttttac cccctcttgg ttatccgttt taacgctaac
    29041 aactgttcgc tcatcgtcat aagagcccgc cttgagaaga atcttcttct catttttaag
    29101 atggtcattg accgcagctg gtagagtcac tgtgtcaaag agattgacat cgttattgct
    29161 ggcatttagc tgaccgtctg acttgagagt gatagaaaga cggtttattg atcctgtttc
    29221 aggagcagca acacggttgc ctttatacca tgtgctatta cctggagttt tgtattccca
    29281 gaaccagcca tccttgtcat aaccgacaaa aacattatta ttggtatctt taaatttcaa
    29341 gaagacacca aagcgtgatt tgcccttttc agaatcttct ttgaaggtta aatcaacagt
    29401 tgcatttcca ttggcatcaa cggtcaagcc cttcttttca aacagggctg gtttgcctgc
    29461 gttatcattt tgagcagttg aggatagttg gttgtagcgg acaccttttt cttctcggat
    29521 agtgactgtt ccctgttgtt cttttttctc taccgtttgc cattcaggag ttaccgtctt
    29581 aggggttacc gtcttagggg tttcaggttt agcagctgca ggtttatcct cttttggttt
    29641 ttcttcagcc ggtttttctt tttcaagtgc taatagttct tcttctgtct taggtccatc
    29701 tgtaacttct ggagaacctt ggtcttctcc caccttaggc gcttcaaaat cacgcccatc
    29761 attctctttt gctgttgcaa gagcagtagc taaatcagct ggtaagttcg ccgtggaacc
    29821 agactgcacg ctatctgcaa gaaccggact tgtcccaaag aatgcagctc caatcataac
    29881 agaagcaaca cctaatgaaa atttccgaat actatattta caacgttttt caaataatcc
    29941 tttattcata aacttctaaa tcctttcatt tttgagcaag cgtttacatt tcctttttaa
    30001 aaaatactcc ttcctctgaa aatgcaatgc ttaaccctga taaataaaaa taattatact
    30061 tttaatataa caaataataa ctgagaaagc aataggaaac aagtaaaatt aacaaaaatt
    30121 ttctctattc tgacaaaaag aggctccaac ttttgggggt cagtacattt agaatctttc
    30181 tttacaaatg taaaaaagga ttcagcattc aagagaaata ctaaatcctt tcttatattt
    30241 tgcaacagtt atttcacatg ttttgcgaga tcttcttgag cctttttatt agactcttct
    30301 ttttctttca tccatttttc ctgagctttt tggtattcat ctacagtgac tgccttgtct
    30361 tgaagttcca agtatttata taagattggt tcacttgtac ctttatttcc tgacaatgca
    30421 aatggtattg taaatggtac catcttagac aagattggac gccctgtacg agatgtagtt
    30481 ggaataatca aagcactatc tgtcaaccaa gcttgggctg cagcgtattt atcatagcgt
    30541 ttagcaacat ctgtagcctc atcaccagcc tcagtaacca atttttcgta gtcatataga
    30601 cctacttttt tagcagctac attatcttcc cctgagtcaa accctaaata tgttttagta
    30661 ctttctccta cagatggttt gatgatatca aggtaggttg atggatcggc aaagtctgga
    30721 ccccaaccga cattatctga taaatcccag tcttcgccag cagcattttc agcaaaatat
    30781 gtaatattgt ttacttcgtc tttttgtagt tgttggatat caataatgac attatcagct
    30841 cctaaagttg cttccaagga ttgtttcata gattggacgc gctgaacttt tgtagttgct
    30901 gtctggtcaa ctggcatatc caaatgaatt gggaaagtca caccttctgc ttgtaaggct
    30961 gatttagctt tagcaaattc agccttggct ttttctggat tgtaaagacc atcctgagaa
    31021 tctgcaagat taacatcctt ccattcatcc ccataagtaa ccaatttctc tttgaccata
    31081 tcgccaaagt ttttaccatc tgcttgaaca aatgttggtg gaacaaagat attacgtaag
    31141 attttgcttg ctccagtttg tccattcaac tgagaggcat aggctgtacg atcaaaacca
    31201 aaggcaatag cctgacggaa atccttgttt aagagagcct ttttagtcga tgccttttgt
    31261 tcgtcgctgg tcttagatgt gtatttatag gactgacggt caatatttgt accaactaaa
    31321 tacgtaatag agtcttgttg agtatagaca atattgtcct tcatactctt ctcaagctct
    31381 gcgaaacttg cacttgttgg atagagacga gctgctgtaa ggctaccatc tttaaagttt
    31441 tctgcaggtt tgctggtatc ttgaccatcc cagaatgaca atttaacttt gtcaatatgc
    31501 acattgtcct tatcccagta gttcggattt ttcgcaaatt caacagaaga tttggtcaca
    31561 atggatttca acaaataagg accgttatac aagagactat ttggatccgt agctttggca
    31621 aaatcatccc cttttgaatt caaaaactct tcattaactg gagccattac tcccattgtt
    31681 gtcttagaat tccagaagct ttctggtttg ttcaaagtgt actgaactgt ctgttcatcc
    31741 agagccttaa ttcctacttg tgagaaatct ttgatttccc cttttacata gccatccaat
    31801 cctttgattg attcttgaac aaggtaaaga gcatctgatt ttttatcagc agcatatttt
    31861 agtcctgtta caaagtcttg agctttgact gccgcgtatt cttcaccttc agaagtatac
    31921 cattttgcat ccttacggat agtataagtg taagtcaatc catccttgga tacagaccaa
    31981 tcctcagcca tagacggcac aaagttcccg tagcgatcat tttctagcaa accatcaacc
    32041 acgttactgg taatatttgc tgtcgcagcc ttagcagttg tcaaatagtt gaggttatca
    32101 gggtctgtct cataaatgta tgagaatgtc ttctcacctt tagtgcttga acctgatcca
    32161 gagcatgcag ctaaagtagt cgccgccaat aatgtcacgc ccgcaagggc aagtagtttt
    32221 gaacttttca tcattttctc ctttaaaact ttctctccat tatagactct tttcagaaag
    32281 atgtcaacag aattttcaga atttttgaaa attatttttc aaacaacatc tttgcaaaaa
    32341 atatgaatat cgtaaccgcc caataacga
//
LOCUS       DAVKMU010000022        28837 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKMU010000022 DAVKMU010000000
VERSION     DAVKMU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28837
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.022"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            100..597
                     /locus_tag="VW343_001156"
     CDS             100..597
                     /locus_tag="VW343_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9755713.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            597..2252
                     /gene="dnaX"
                     /locus_tag="VW343_001157"
     CDS             597..2252
                     /gene="dnaX"
                     /locus_tag="VW343_001157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9755714.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATDKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2280..2474
                     /locus_tag="VW343_001158"
     CDS             2280..2474
                     /locus_tag="VW343_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9755715.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2624..3394
                     /gene="sufC"
                     /locus_tag="VW343_001159"
     CDS             2624..3394
                     /gene="sufC"
                     /locus_tag="VW343_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9755716.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3422..4684
                     /gene="sufD"
                     /locus_tag="VW343_001160"
     CDS             3422..4684
                     /gene="sufD"
                     /locus_tag="VW343_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9755717.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4695..5921
                     /locus_tag="VW343_001161"
     CDS             4695..5921
                     /locus_tag="VW343_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9755718.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATAFYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVINPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5908..6348
                     /locus_tag="VW343_001162"
     CDS             5908..6348
                     /locus_tag="VW343_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9755719.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6402..7814
                     /gene="sufB"
                     /locus_tag="VW343_001163"
     CDS             6402..7814
                     /gene="sufB"
                     /locus_tag="VW343_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9755720.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8045..9286)
                     /locus_tag="VW343_001164"
     CDS             complement(8045..9286)
                     /locus_tag="VW343_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9755721.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQYGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9389..10498
                     /locus_tag="VW343_001165"
     CDS             9389..10498
                     /locus_tag="VW343_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9755722.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11032..11379
                     /gene="ssrA"
                     /locus_tag="VW343_001166"
     tmRNA           11032..11379
                     /gene="ssrA"
                     /locus_tag="VW343_001166"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11552..11639)
                     /locus_tag="VW343_001167"
     tRNA            complement(11552..11639)
                     /locus_tag="VW343_001167"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11603..11605),aa:Ser,seq:gga)
     gene            11802..12542
                     /locus_tag="VW343_001168"
     CDS             11802..12542
                     /locus_tag="VW343_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9755723.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12539..13450
                     /gene="pfkB"
                     /locus_tag="VW343_001169"
     CDS             12539..13450
                     /gene="pfkB"
                     /locus_tag="VW343_001169"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9755724.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTVEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13447..15399
                     /locus_tag="VW343_001170"
     CDS             13447..15399
                     /locus_tag="VW343_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9755725.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSAGQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15563..17788
                     /locus_tag="VW343_001171"
     CDS             15563..17788
                     /locus_tag="VW343_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9755726.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKTLLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(17833..18261)
                     /locus_tag="VW343_001172"
     CDS             complement(17833..18261)
                     /locus_tag="VW343_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755727.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITAGITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            18399..19541
                     /locus_tag="VW343_001173"
     CDS             18399..19541
                     /locus_tag="VW343_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9755728.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19550..20764
                     /gene="thiI"
                     /locus_tag="VW343_001174"
     CDS             19550..20764
                     /gene="thiI"
                     /locus_tag="VW343_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9755729.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGRAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            20899..21722
                     /locus_tag="VW343_001175"
                     /pseudo
     CDS             20899..21722
                     /locus_tag="VW343_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            21744..22483
                     /locus_tag="VW343_001176"
                     /pseudo
     CDS             21744..22483
                     /locus_tag="VW343_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            22496..23662
                     /locus_tag="VW343_001177"
     CDS             22496..23662
                     /locus_tag="VW343_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9755730.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     ATTFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            24170..25663
                     /locus_tag="VW343_001178"
     CDS             24170..25663
                     /locus_tag="VW343_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9755731.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            25676..26740
                     /locus_tag="VW343_001179"
     CDS             25676..26740
                     /locus_tag="VW343_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9755732.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAY
                     GGNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLV
                     FLLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFADFVALVDKSQLAI
                     QKSLEELETLKKSLMQEYFG"
     gene            26800..27036
                     /locus_tag="VW343_001180"
     CDS             26800..27036
                     /locus_tag="VW343_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9755733.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            27033..27446
                     /locus_tag="VW343_001181"
     CDS             27033..27446
                     /locus_tag="VW343_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9755734.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            27564..28529
                     /locus_tag="VW343_001182"
     CDS             27564..28529
                     /locus_tag="VW343_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9755735.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            28514..28837
                     /locus_tag="VW343_001183"
                     /pseudo
     CDS             28514..28837
                     /locus_tag="VW343_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         8788 a         5222 c         6275 g         8552 t
ORIGIN      
        1 ggggttaccc actacaaata ttatagagcc caaaatcctt tgtttactaa acaagggatt
       61 tttcttttgt ctctgctcct tttttgatat aatagttcta tgttaaaatc agaaaaacaa
      121 tcacgttatc aaatgttaaa tgaagaattg tccttcctat tggaaggcga aaccaatgtt
      181 ttggctaatc tttccaacgc cagtgctctc ataaaatcac gttttcctaa taccgtattt
      241 gcaggctttt atttgttcga tggaaaggaa ttggttttag gccccttcca aggaggtgtt
      301 tcctgcatcc gtattgcact aggcaagggt gtttgtggtg aggcagctca ctttcaggaa
      361 actgttattg ttggagatgt gacgacctat ctcaactata tttcttgtga tagtctagct
      421 aaaagtgaaa ttgtggtgcc gatgatgaag aatggtcagt tacttggagt tctggatctg
      481 gattcttcag agattgagga ttacgatgct atggatcgag attatttgga acaatttgtc
      541 gctattttgc ttgaaaagac aacatgggac tttacgatgt ttgaggaaaa atcttaatgt
      601 atcaagcact ttatcgaaaa tatagaagtc aaaacttctc ccagttagtt ggtcaagaag
      661 ttgtggctaa gactcttaaa caagcggtgg agcaagagaa aataagtcac gcttatcttt
      721 tttctggtcc tcgtggaacg ggaaaaacca gtgttgctaa aatctttgcc aaggctatga
      781 actgtcccaa tcaagtgggt ggcgaacctt gcaataactg ctatatttgt caagcagtga
      841 cggacggtag tttagaagat gtcattgaaa tggatgcagc ttctaataat ggggtagatg
      901 aaattcgcga aattcgtgat aaatctacct atgcgcctag ccttgctcgt tataaggttt
      961 atatcataga tgaggttcac atgctgtcta caggggcttt taatgccctc ctaaagacgc
     1021 tggaagaacc aacacagaat gtagtcttta ttttggccac tactgaattg cacaagattc
     1081 ctgctactat tctatcccgt gtgcaacgtt ttgagtttaa atcaattaag acacaggata
     1141 ttaaggaaca tattcactat atcttagaaa aagaaaatat cagttctgaa ccagaggctg
     1201 tggaaatcat tgccagacgg gcggaaggtg gaatgcggga cgccttgtct attttggatc
     1261 aagccctgag tttgacacag ggaaatgagc tgacgactgc tatctctgaa gaaattactg
     1321 gcaccattag cctatcagcc ttggatgatt atgtggcggc cttgtctcaa caggatgttc
     1381 ccaaagcttt gtcttgcttg aatcttcttt ttgacaatgg taagagcatg actcgttttg
     1441 tgaccgatct tttgcactat ttaagagact tgttaattgt tcaaacaggg ggagaaaata
     1501 ctcatcatag ttcagtcttt gtagaaaatt tggcacttcc tcaaaaaaat ctgtttgaaa
     1561 tgattcgctt agcaacagtg agtttagcag atattaagtc tagtttgcag cccaagattt
     1621 atgctgaaat gatgaccgtc cgtttggcgg aaatcaagcc cgaaccagct ctatcaggag
     1681 cggttgaaaa tgaaattgct acgctgagac aggaagttgc ccgtctcaaa caagagcttt
     1741 ctaatgtagg tgcggttcct aaacaagttg caccagctcc tagtcgacca gctacggaca
     1801 aaacagtcta tcgtgtcgat cgcaataaag tgcaatctat cttacaagag gccgtcgaaa
     1861 atcctgattt agcacgtcaa aatctaattc gtttgcagaa tgcctgggga gaggtaattg
     1921 aaagtctagg tgggccggac aaggctctgc tagttggttc tcaaccggtt gctgccaatg
     1981 aacaccatgc tattcttgct tttgagtcta acttcaatgc tggtcaaact atgaaacgag
     2041 acaatctcaa taccatgttt ggtaatatcc tcagtcaggc ggcaggtttt tcacctgaga
     2101 ttttagctat ttccatggag gaatggaaag aagttcgcgc agccttttca gccaaagcca
     2161 aatcttctca aactgaaaaa gaagtagaag aaagcctgat tccagaagga tttgaatttt
     2221 tggctgataa agtgaaggta gaggaagact aaagaaagat ttcatgatac aataagttta
     2281 tgaataaaca acaatttatt attatggcgc tgtttacagc tgctgagacc tattttttca
     2341 atgaagcctg gatgactggc cgctatatta tggcagcctt ttgggcaatt ttactcttta
     2401 gaaatttccg agtcagttat gtgatgggca aaatcgttga tgtcatcgat cagcatttta
     2461 ataggaaaga ctagccctca gcttccagac aaaatcaaag ccttttaggc ttttttttgt
     2521 tatactagaa aagtatattt atagaatttt tgctctattt ctggggaaat cagacgtttt
     2581 tctagtaagt actgtaaaag ttttgaaaaa gaaaggaact atcatgtcag tattagagat
     2641 taaagatctt cacgttgaga ttgaaggaaa agaaatttta aaaggggtta acctgaccct
     2701 gaaaacagga gaaattgccg ctatcatggg accaaatggt acaggtaaat cgactctttc
     2761 tgccgctatc atgggaaatc caaactatga agtaactaaa ggtgaagttt tgtttgatgg
     2821 cgtaaacatc cttgagttgg aagtggatga gcgtgcgcgt atgggacttt tccttgctat
     2881 gcaataccca tcagaaatcc ctggaattac caatgctgag tttcttcgtg ccgctatgaa
     2941 tgcgggtaaa gaagatgatg agaagatttc agttcgtgag tttattacta agctagatga
     3001 aaaaatggaa ttgctcaaca tgaaagaaga aatggcagag cgttacctca acgaaggctt
     3061 ctctggtggt gagaaaaaac gcaatgaaat tcttcaactt ttgatgttgg agccaacatt
     3121 tgctcttttg gacgagattg actcaggtct tgatattgac gctcttaaag ttgtgtctaa
     3181 aggtgtcaat gccatgcgtg gtgaaggttt tggtgctatg atcatcactc actaccaacg
     3241 tcttttgaac tatatcacac ctgatgtggt acacgtgatg atggaaggtc gtgttgtcct
     3301 ttctggtggt ccagaattgg ctgcgcgttt ggaacgtgaa ggatacgcaa aattagctga
     3361 agaacttggc tacgactaca aggaagaatt gtaatttcct cgtatctttt aggagaagta
     3421 aatgactaga gaaaatatta aacttttttc agaaatgcac gctgaaccaa gctggttggc
     3481 tgatctccgt caaaaagctt ttgacaagat tgagactttg gaattaccag ttattgagcg
     3541 tgtcaaattc caccgttgga atctgggtga tggaacgatt acagaaaatg agccatcagc
     3601 aaatgttcca gatttcacag ctttagatca tcacttgaag ttggtgcaag taggaactca
     3661 aactgttttc gaacaaactc cagttgagtt agctgaacag ggtgttgtct tcacagactt
     3721 tcactcagct ttagaagaaa ttccagagct gatcgaagaa ttcttcatgt catctgttaa
     3781 gtatgatgat gacaagttgg cggcttacca cacagcttac tttaacagtg gtgctgtact
     3841 ctatattcca gataacgtag aaatcacaga gccaattgaa ggaattttct accaagatag
     3901 cgatagcaat gtgccgttta acaagcatat tatgattatc gctggtaaaa attctaagat
     3961 tagttatctg gagcgtttag agtcacgcgg tgaaggaagt gacaaagtaa ctgccaatat
     4021 cacagtggaa gtgattgcac gttctggtgc gcaagtcaag tttgctgcta tcgaccgtct
     4081 aggtgaaaac gtcactgcct acattagccg tcgtggtaaa ttaggcaacg atgcaagtat
     4141 tgactgggct atcggtgtca tgaacgaagg aaatgtcgtt gctgattttg atagtgactt
     4201 gattggtaat ggtagccatg ctgacctcaa ggttgtagct ctttcaagtg gtcgtcaggt
     4261 acaagggatt gatactcgtg taactaacta tggctgcaac tctatcggaa atatcctaca
     4321 acatggggtt atccttgaaa aagcaacttt gactttcaat ggtatcggcc acatcatcaa
     4381 gggtgctaag ggagcagatg cgcaacaaga gagccgtgtt ctcatgcttt cagaccaagc
     4441 gcgttcagat gctaacccaa ttcttttgat tgatgaaaat gatgtcactg caggacacgc
     4501 agcgtctatc ggacaagttg atccagaaga catgtattac ctcatgagtc gtggattgga
     4561 taaggcaact gcagagcgtt tggttgttcg tggtttcctt ggatctgtta tcgtggagat
     4621 tccagtcaag gaagttcgtg atgaaatgat tgcaactatc gaagagaaat tgtcaaaacg
     4681 ctaaggggaa gcctatgtta gatgtagaag cgattcgcaa ggattttcca attttagatc
     4741 agattgtcaa tgatgaacct ctggtctatc tggacaatgc tgcgacgaca caaaaacccc
     4801 tagtagttct gaaagctatt aacagttact atgagcagga caatgccaat gttcaccgtg
     4861 gtgtccatac cttagcggaa cgagcgacag ctttttatga agctgctcgt gaaaccattc
     4921 gtaagtttat taatgcaggc tctacaaagg aagttctctt taccagagga acgacaacca
     4981 gccttaactg ggtggcacgc tttgctgagg aaattctcac tgagggagac caggtcttga
     5041 tttcagtaat ggaacaccat tctaatatca ttccatggca ggaagcttgt cgaaagactg
     5101 gagcagagct tgtctatgtc tatcttaaag acggtgcctt ggatatggag gatttgcgag
     5161 ctaaattgac tgataaggtt aaatttgttt ccctagctca tgcctccaat gttcttggtg
     5221 tgatcaatcc gatcaaggaa atcactcaat tagcccacca agttggggca atcatggtag
     5281 tggatggtgc tcaatctaca cctcatatga agattgatgt ccaggacttg gatctggact
     5341 ttttcgcctt ttcgggtcac aagatggctg gtccgactgg tatcggtgtc ctttacggca
     5401 aagaaaagta tcttgagcaa atgtctccag tagaatttgg cggcgagatg attgattttg
     5461 tctacgagca atttgctagt tggaaggaat tgccttggaa atttgaggct ggaacgccaa
     5521 atatggcagg agctattgga cttgcgactg cagttgatta tttggaaaag attggtatgg
     5581 atgccgttga agctcatgaa caggaattga ttgcgtacgt ctatccaaaa ctgcaggcaa
     5641 ttgagggatt gaccatttac ggttctcagg atttggctca acgttcgggt gttattgcct
     5701 ttaacctagg tgatctccat cctcacgatc ttgcgacggc tctggattat gaaggagtgg
     5761 ctgttcgtgc tggtcaccat tgtgcgcaac ccttgcttca gtatttggaa gtcccagcaa
     5821 cagctcgtgc aagtttttat atctacaata ccaaggcaga ttgcgacaaa ctagtcgatg
     5881 ccctacaaaa gacaaaggag tttttcaatg gcactttcta aactagatag cctttatatg
     5941 gcagtggtag cagaccattc gaaaaatcca catcaccaag ggaagttaga agatgctgag
     6001 caaatcagtc tcaacaatcc gacttgtggg gatgtcatca acctctctgt caagtttgat
     6061 gcagaggacc gtttggaaga tattgctttt ctaaattcag gatgcacgat ttcaactgct
     6121 tctgctagta tgatgacaga tgccgtttta ggaaaaacca aacaagaaat tttagaactg
     6181 gcgactattt tttctgaaat ggttcaaggg caaaaagatg agcgtcaagg ccaacttgga
     6241 gacgcggcat tcttgtcagg tgttgccaaa ttccctcaaa gaatcaagtg tgcaacccta
     6301 gcttggaatg cccttaagaa aacaattgaa aatcaagaaa aacagtaaga caagtttctt
     6361 ttgtcttatg aattattaga aatgaagaaa gaaaggatac tatggctgaa gaaagagtag
     6421 aaccaaaacc aattgacctt ggtgaatata aatttggttt ccatgacgat gtagagcctg
     6481 tcttatcgac aggaaaagga ctcaacgaag gtgttattcg tgaattatct gctgctaagg
     6541 gtgagcctga gtggatgttg gagttccgtt tgaagtctta tgaaaccttc aaaaaaatgc
     6601 ccatgcaaac ttggggagca gacttgtcag agattgactt tgatgactta atctactacc
     6661 aaaaaccatc tgacaaacca gcccgttctt gggatgatgt acctgaaaag attaaagaaa
     6721 cctttgaacg tatcgggatt ccagaagctg aacgtgctta tttagcaggg gcttctgccc
     6781 agtacgagtc agaagtggtt taccacaaca tgaaggaaga gttccaaaaa ttaggtatta
     6841 tctttacaga tacagattcc gcactcaagg aatacccaga cttatttaaa caatactttg
     6901 cgaagttggt accgccgaca gataacaagt tggcagccct caactcagca gtatggtcgg
     6961 gtggaacttt tatctacgtg ccaaaaggtg tcaaggtaga tattccactt caaacttatt
     7021 tccgtatcaa taacgaaaat ataggtcagt tcgaacgtac cttgattatc gttgatgagg
     7081 gagcaagcgt ccactacgta gaaggatgta cagcaccaac atattcaagc aatagcttac
     7141 acgctgccat tgtagaaatt tttgctttgg acggagctta tatgcgttat acaactatcc
     7201 aaaactggtc tgataacgtc tataacttgg taacaaagcg tgctaaggct caaaaggatg
     7261 ccactgttga gtggattgat ggaaacttgg gtgccaaaac gactatgaaa tatccatctg
     7321 tttaccttga tggagaagga gcgcgtggta ccatgctctc tatcgccttt gctaatgcag
     7381 ggcaacacca agacacgggt gctaagatga ttcacaatgc tccacatacc agctcgtcta
     7441 ttgtgtctaa atccatcgct aaaggtggag gaaaggttga ctaccgtgga caagtcacct
     7501 ttaacaagaa ctctaagaaa tctgtttccc acattgaatg tgataccatt atcatggatg
     7561 acttgtcagc atcagatact attccattta atgaaattca caactcgcaa gtggctttgg
     7621 aacacgaagc caaagtatct aagatttcag aagagcaatt gtattatctc atgagccgtg
     7681 gattgtcaga atctgaggca actgaaatga ttgtcatggg atttgtagaa ccctttacaa
     7741 aagaacttcc aatggaatac gcagttgagc tgaaccgctt gattagctat gaaatggagg
     7801 gatcagttgg ataaaatttg attttatact cttcgaaaat ctcttcaaac cacgtcagca
     7861 tcgccttacc gtatgtatgg ttactgactt cgtcagtttc atctacaacc tcaaaacagt
     7921 gttttgagca agctgcggct agcttcctag tttgttcttt gattttgagt attagattta
     7981 ctcaaaatca aggattttga agatgaactt gtatcaaaaa atcgcggttt aaaatcgcga
     8041 ttttttataa tttctcgtta acaaagcgga caaactgatt ccaccaaact tttaagaaga
     8101 aggctttttc aattttcttg tctgctacca tttcgaaact agggcgctct gtggtgatgt
     8161 aaccttgacc aatcaagtcc ttgtcttcat aagtcaaatg gccaaccact gttccagctt
     8221 caagtggtgc tgggattgct ttggaatcag gtgtgaattg aacagattgg gaagattgat
     8281 tcccaacacg ttcgattaga tagatatcct ctggagccac tgcagttact gtatcttctt
     8341 ttccatattg tacaggggtt ttgctatctt gataggcatc gccttgttga acgattttgc
     8401 gaagtgtaaa tgtagaagaa atataatcca ttagggaaga tgtagctgta aatcgagcgt
     8461 aaggattatt gtcttgatga tctgcattta aaacaactgt gatgactctc atgccttttt
     8521 cgacagtagt accaacaaaa gactctccag ccttatctgt tgttcctgtt tttagcccat
     8581 caaaaccacc acggtaagca ggcatacctt ctaacatgta gttggttgaa gtgattgtca
     8641 tcccagcaaa agtagaagaa ggttttttgg tgatttctaa gacttgtggg tattttttga
     8701 tgaggttgcg agcaacgata gcgacatcat aagcactaag cttattttcc tcatcttttt
     8761 tagaacctgg gtaaatgtta tcccctagag tttcattgtt aagacctgtc gtattgacaa
     8821 cagtggcatc ctgaattccc cattccaaga gttttgcccg catcatatcg acgaaatctt
     8881 tttctgagcc agcaattttc tcagctaggg caatagcggc gctgttggca ctagatacca
     8941 gagttgcttc aagcaactct tcgacagtat aattacgggc ctccatagga atattactgg
     9001 cttcagaatt tgtcgtcaat tgataaggat aatcagaaat atctacagga gtggagaggg
     9061 taatacttcc gttttccaaa gcttcataga ccagataaac agtaatcaat tttgttatgg
     9121 aagcaatttc gacaggttgc gttgcatcct tctcatagag aattttacca gtatttgcct
     9181 caacagcaat cgcatgttta gcggcaatgg taaaatcttg agcaacagca gtagaagcac
     9241 cccctaaaag agagacagtt aacaaagtta aaaatatttt tttcatagta gtcttattct
     9301 atcataaaga aaaaaaatat tcttgcttta ataattcatc tgttaagctt tttgaaaata
     9361 tggtaaaata aagtgaggga ggtaactcat gtttcgtaga aataaattat ttttttggac
     9421 cacagaaatt ttactcttaa ccatcatctt ttacctatgg agacagatgg gatctttgat
     9481 taaccctttt gttagcgtgc ttaatacaat tatgattcca tttttattag ggggctttct
     9541 ttattatttg acaaacccta ttgttacttt cttaaataaa gtctgtaaac tcaatcgttt
     9601 gcttggtatt ttaattacct tgtgtacttt ggtctgggga atggtcatag gtgttgtcta
     9661 tctcttacct attttgatta atcagttatc tagtttgatt atatctagtc aaactattta
     9721 tagtcgagta caagacttaa tcatagactt atctaattat cctgcgctcc agaatttgga
     9781 tgtagaagct acaattcagc agttaaactt atcctatgtt gatattcttc aaaatatcct
     9841 aaatagcgta tcaaatagtg tggggagcgt cttgtcagct cttatcagta ctgttttgat
     9901 tttgattatg actccagttt ttttggttta tttcttatta gatggacata aattcttgcc
     9961 catgcttgaa agaacgattc taaagaggga tcgcttgcat attgcaggct tattaaagaa
    10021 tttaaatgcg acgattgctc gctatattag tggagtttcg attgacgcaa tcattatagg
    10081 ttgtttggct tatattggct atagtattat tggtttaaaa tatgctttag tttttgccat
    10141 tttttctggt gtagccaatt taattcctta tgtggggcca agtattggtt tgattcctat
    10201 gatcatcgca aatatattca ctgatcccca tagactgctg attgcagtga tttatatgct
    10261 tgttgttcag caggtagatg gcaatatctt atatcctcga atcgtaggaa gtgttatgaa
    10321 ggttcatcca atcacgattt tagttttact tttgttgtca agcaatatct atggtgtagt
    10381 tggaatgatt gtcgcagtgc caacctattc tatcttgaaa gaaatttcta agttcttatc
    10441 ccgtttgtat gaaaatcata aaataatgaa agaacgagaa agagaattag ctaagtaaaa
    10501 gtcaggagaa ccctgatttt tctttactgg aagtggcctt tagattagaa gactgaaaat
    10561 aagttaaagt cttaaactaa ttttcacagc taagaatagt agaagttaat ctgataaaaa
    10621 tcgaaaaaac cagtggaatt ctgtgtcagg gtaagttcca ctggttttca tagtctatta
    10681 aagttcgaat gaaacctatt tatagtagat tgaaactaga atagtacacc tctaattcta
    10741 aaacattgtt agaaatcgat ttgactgtcc tgatctattc gtcctattct tatttcattt
    10801 tactatattt tggtgcaata agtgaaaagt agaccgaata atataaggat tgattttata
    10861 gtttttaaac tcaaatgaat tgaaataaag agagtacgaa aattctcatc tgaaagtatt
    10921 ttagaataat tctcttcgtg aatttcttca aaacagatag cttcatctta ggtatgtgat
    10981 ttctttttgc atttttgagt tagataaggt ataatggttt tattgtcttt tggggtcgtt
    11041 acggattcga caggcattat gaggcatatt ttgcgactcg tgtggcgacg taaacgctca
    11101 gttaaatata actgcaaaaa ataacacttc ttacgctcta gctgcctaaa aaccagcagg
    11161 cgtgacccga tttggattgc tcgtgttcaa tgacaggtct tattattagc gagatacgat
    11221 taagccttgt ctagcggttt gataagagat tgatagactc gcagtttcta gacttgagtt
    11281 atgtgtcgag gggctgttaa aataatacat aacctatggt tgtagacaaa tatgttggca
    11341 ggtgtttgga cgtgggttcg actcccaccg gctccattat tcctttgcat tccttggtaa
    11401 aacgttgtta aatcaacgtt ttttattttt atctttggta ttcctttgca ttcttttgct
    11461 aaaaagggag tcacaaacag accctatttt aaaaaaagga tagaaaaaag gatacaacat
    11521 ttgtcgcatc ctaaaaataa tcttttttcg acggaagaca tgggattcga acccacgcac
    11581 gctattacac gcctaccgcg tttccaacac ggcctcttaa gcctcttgag taatcttcca
    11641 atacttactc aaatagtcta ccataaaggc tcttatcttg caataaaaat tctagaaata
    11701 agaaaaaatg atagattttg aaagaaaatg ataaaaaatg cttgacttcg aaagaaagta
    11761 tgatagaatg aatagtgtaa acgataacag gaggtgattc agtgttaaaa acagaacgta
    11821 aacaactaat tttagaggag ttaaatcagc atcatgtagt ttctctagaa aaattagtta
    11881 gtttgctaga aacgtcagaa tcaacggttc gaagagactt ggatgagttg gaagcggaaa
    11941 acaagcttcg tcgtgtgcat ggtggagcag aactccccca ctccttacag gaagaagaaa
    12001 ccattcaaga aaaatctgtc aaaaaccttc aagaaaagaa gttgctggct cagaaagcag
    12061 cctctctcat taaagaaaaa gatgtcatct ttatcgatgc tggaacaaca actgcttttt
    12121 tgattcatga attggtcaat aagaatgtta cagttgtgac caactccatt caccatgccg
    12181 ctcagttggt tgaaaagcag attccaactg tcatggttgg aggaaacgtc aagacggcga
    12241 cagatgctag tatcgggggc gttgctctta accagattaa ccaattgcac tttgaccgtg
    12301 cctttatcgg aatgaatggt gttgacgatg gctattatac gactcctgat atggaggagg
    12361 gagctgtgaa aagagctatt ttggagaatg ccaagcagac ctacgtcttg gtggattcgt
    12421 caaaaattgg acaaacttgc tttgccaagg tagcaccact caaacgcgct atcgttatca
    12481 ctagtcaagg gcatgagctc ttgcaggtta ttaaggagaa aacggaggta atagaagtat
    12541 gatttataca gtcacactca atccatccat tgactatatc gttcgtttgg accaagtcaa
    12601 agttggtagt gtcaatcgta tggacagtga tgataagttt gctggtggga aaggaatcaa
    12661 tgtcagccgt gtcttgaaac gtttgaatat accaaataca gcgacgggat ttatcggtgg
    12721 ctttactggt aaatttatca cagatactct agcagaggaa gaaatcgaga cacgttttgt
    12781 ccaagtggca gaagatactc gtatcaatgt taaaatcaaa gcagaccaag aaacagaaat
    12841 caacggaacg ggtccaactg ttgaaccggt tcagctagaa gaattgaaag ctattttatc
    12901 tagtctgaca gtagaagata cagttgtctt tgcaggttca agtgctaaaa atctaggcaa
    12961 tgttatctat aaggatttga tttccttgac gcgccagact ggtgcgcaag tggtctgtga
    13021 ctttgaagga cagaccttaa ttgatagttt ggactaccag cctcttcttg taaaaccaaa
    13081 caatcatgaa cttggagcga tttttggggt taaactcgaa agtttagatg aaattgagaa
    13141 atacgctcgt gagttactgg ctaagggtgc tcaaaatgtt attatctcta tggctggtga
    13201 tggcgccctt cttgtcacat ctgagggagc ttacttcgct aaaccaatca aaggaacagt
    13261 caaaaattca gttggagctg gtgattctat ggttgctgga ttcacaggtg aatttgtcaa
    13321 atcaaaagac gcagtagaag ccttcaaatg gggagtggct tgcggaacgg caactacctt
    13381 ctcagatgac ttggcaacgg cggaatttat taaagaaaca tatggaaaag ttgaggtaga
    13441 aaaaagatga aaattcaaga cctattgaga aaagatgtca tgttgctaga tttgcaggca
    13501 actgaaaaaa cagctgtcat cgacgagatg attaaaaatt tgacagagca cggttatgta
    13561 acagattttg aaacatttaa agaaggaatt ttggcgcgtg aagctttgac ttctactggt
    13621 ttgggtgatg gaatcgcaat gcctcacagc aaaaacgctg ctgtcaaaga agcgacagtt
    13681 ctatttgcta agtcaaacaa gggtgttgac tacgagagct tggatggaca agcaactgac
    13741 ctcttcttca tgattgcagc tccagaaggt gccaatgata ctcacttggc agccttggca
    13801 gaattgtctc aatacttgat gaaagacggt tttgcagaca aacttcgtca agcaacatct
    13861 gcaggccaag ttatcgaact ttttgaccaa gcttcagaaa aaactgagga acttgttcaa
    13921 gcacctgcta atgactctgg tgactttatc gtagctgtta cagcttgtac aacaggtatt
    13981 gcccacactt acatggccca agaagccctt caaaaagtag ctgctgaaat gggggttggt
    14041 atcaaggtcg aaaccaacgg tgctagcggt gttggaaatc aactaactgc agaagatatc
    14101 cgtaaggcta aagctattat cattgcagca gacaaggccg ttgaaatgga tcgatttgat
    14161 ggaaaaccat tgatcaatcg tccagttgct gacggtatcc gtaagacaga agagctaatt
    14221 aacttggctc tttcaggaga tactgaagtc taccgtgccg ctaatggtgc caaagctgca
    14281 acagcctcta acgaaaaaca aagccttggt ggtgccttgt acaaacactt gatgagtggt
    14341 gtatctcaaa tgttaccatt cgttatcggt ggtggtatca tgattgccct tgccttcttg
    14401 attgacggtg ctttgggtgt tccaaatgaa aaccttggca atcttggttc ttaccatgag
    14461 ttagcttcta tgtttatgaa aattggtgga gctgcctttg gtttgatgct tccagtcttt
    14521 gcgggttatg ttgcctactc tattgctgaa aaaccgggtt tggtagcagg tttcgtggct
    14581 ggtgctattg ccaaagaagg ttttgccttt ggtaaaattc cttatgccgc aggtggtgaa
    14641 gcaacttcaa ctcttgcagg tgtctcatct ggtttcctag gtgcccttgt tggtggattt
    14701 atcgcaggtg ccttggttct tgccatcaag aaatacgtta aagttcctcg ttcactcgaa
    14761 ggtgctaaat caatccttct attgccactt cttggaacaa tcttgacagg atttgttatg
    14821 ctagctgtga atatcccaat ggctgcaatc aacactgcta tgaatgactt cctaggcggt
    14881 cttggaggag gttcagctgt ccttcttggt atcgtccttg gtggaatgat ggctgttgac
    14941 atgggtggac cagttaataa agcagcttat gtctttggta caggtacgct tgcagcaact
    15001 gtttcttcag gtggttctgt agccatggca gcagttatgg ctggaggaat ggtgccacca
    15061 cttgcaatct ttgtcgcaac tcttcttttc aaagataaat ttactaagga agaacgtaac
    15121 tctggtttga caaacatcat catgggcttg tcatttatca ctgagggagc gattccattt
    15181 ggtgccgctg acccagctcg tgcgattcca agcttcatcc ttggttcagc agtagcaggt
    15241 ggactcgttg gtcttactgg tatcaaactc atggcgccac acggaggaat cttcgttatc
    15301 gcccttactt caaatgctct cctttacctc gtttctgtct tggtaggagc aatcgtaagt
    15361 ggtgtggttt atggttacct acgcaaacca caagcataaa aaatagaaaa atgaaaagat
    15421 tggaccgttt ggtgcagtct ttttctcttc ccgaaatgcc tgtgaaatat ggtataatag
    15481 aagaatggca aacaagaata caagtacaac aagacggaga ccgtctaaag cagaactgga
    15541 aagaaaagaa gcgattcaac gaatgttgat ttcgttagga attgcgattt tattgatttt
    15601 cgcagccttc aaattagggg ctgcaggtat aaccctttat aatttaattc gcttgctagt
    15661 gggtagccta gcttatctgg cgatattcgg cctattaatc tatctcttct ttttcaagtg
    15721 gatacgaaaa caggaaggac tcttatctgg ctttttcacc atatttgctg gcttactctt
    15781 gatttttgag gcctacttgg tttggaaata tggtttggac aagtccgttc taaaagggac
    15841 catggctcag gttgtgacag atctgactgg ttttcgaacg actagctttg ctggaggggg
    15901 cttgatcggg gtcgctcttt atattccaac agcctttctc ttttcaaata tcggaactta
    15961 ctttattggt tctatcttga ttttagtggg ttctctccta gtcagccctt ggtctgttta
    16021 cgatattgct gaatttttca gtagaggctt tgccaaatgg tgggaagggc acgagcgtcg
    16081 aaaagaggaa cgctttgtca aacaagaaga aaaagctcgc caaaaggctg agaaagaggc
    16141 tagattagaa caagaagaga ctgaaaaaac cttactcgat ttgcctcctg ttgatatgga
    16201 aacgggtgaa attctgacag aggaagctgt tcaaaatctt ccacctattc cagaagaaaa
    16261 gtgggtggaa ccagaaatca tcctgcctca agctgaactt aaattccctg aacaggaaga
    16321 tgactcagat gacgaagatg ttcaggtcga tttttcagcc aaagaagccc ttgaatacaa
    16381 acttccaagc ttacaactct ttgcaccaga taaaccaaaa gatcaatcta aagagaagaa
    16441 aattgtcaga gaaaatatca aaatcttaga agcaaccttt gctagctttg gtattaaggt
    16501 aacagttgaa cgggccgaaa ttgggccatc agtgaccaag tatgaagtca agccggctgt
    16561 tggtgtaagg gtcaaccgca tttccaatct atcagatgac ctcgctctag ccttggctgc
    16621 caaagatgtc cggattgaag caccaatccc tgggaaatcc ctaatcggaa ttgaagtgcc
    16681 caactccgat attgccactg tatctttccg agaactatgg gaacaatcgc aaacgaaagc
    16741 agaaaatttc ttggaaattc ctttagggaa ggctgttaat ggaaccgcaa gagcttttga
    16801 cctttctaaa atgccccact tgctagttgc aggttcaacg ggttcaggga agtcagtagc
    16861 agttaacggc attattgcta gcattctcat gaaggcgaga ccagatcaag ttaaatttat
    16921 gatggtcgat cccaagatgg ttgagttatc tgtttacaat gatattcccc acctcttgat
    16981 tccagtcgtg accaatccac gtaaagccag caaggctctg caaaaggttg tggatgaaat
    17041 ggaaaaccgt tatgaactct ttgccaaggt gggagttcgg aatattgcag gttttaatgc
    17101 caaggtagaa gagttcaatt cccagtctga gtacaagcaa attccgctac cattcattgt
    17161 cgtgattgtg gatgagttgg ctgacctcat gatggtggcc agcaaggaag tggaagatgc
    17221 tatcatccgt cttgggcaga aggcgcgtgc tgcaggtatc cacatgattc ttgcaactca
    17281 gcgtccatct gttgatgtca tctctggttt gattaaggcc aatgttccat ctcgtgtagc
    17341 atttgcggtt tcatcaggaa cagactcccg tacgattttg gatgaaaatg gagcagaaaa
    17401 acttcttggt cgaggagaca tgctctttaa accgattgat gaaaatcatc cagttcgtct
    17461 ccaaggctcc tttatctcgg atgacgatgt tgagcgcatt gtgaacttca tcaaggctca
    17521 ggcagatgca gactacgatg agagttttga tccaggtgag gtttctgaaa atgaaggaga
    17581 attttcggat ggagatgctg gtggtgatcc gctttttgaa gaagctaagt ctttggttat
    17641 cgaaacacag aaagccagtg cgtctatgat tcagcgtcgt ttatcagttg gatttaaccg
    17701 tgcgacccgt ctcatggaag aactggagat agcaggtgtc atcggtccag ctgaaggtac
    17761 caaacctcga aaagtgttac aacaataaaa aaatagcttc tttccgagtt tggagggaag
    17821 ctattttagt ggctattgat tgcttttatt ttctgaagtt ggcgcattgg actgtttttc
    17881 gttttcagta gcaggtttac ttgaagcagg agtagaagag tcctgagttg ctgttttctg
    17941 atcttctttt ttctcttcct tgacgctaga ttttggtgtt tcctcttgct gtgttttttc
    18001 ttgactagtg ttagtctctt tagttggact ggtgttttcc ttaggggatt ccttttggat
    18061 ttctttgaca atggttgtcg tctggcttgt cgtaggttct tttttaatat ttttgttatt
    18121 atccaaggcg ttcatgatcg tgatacccgc ggtaatcaag ccaaggatac tagcgattgt
    18181 agcaacggtt ttttgaaaga ctgtaaagcc ttttttgatg tgttctgttt ttggttttga
    18241 agttctacga tagttagaca tacattctct cctttgatta tgatttatta tactctgttt
    18301 ctttaaaaaa atcttaaaaa aagagaaatt gccctttctt gtcgcaggct ccgtttcatg
    18361 atacaataga aggagtgatt ttagaaagcg tgagaaacat gatttacttt gataattcgg
    18421 cgacgaccag gccctatcca gaagcccttg aaacctatat gcaggtggct tcaaaaattt
    18481 taggaaatcc atctagtctc catcgtttgg gagaccaggc aacacgaatt ttagatgctt
    18541 cccgtcaaca gattgcagat ttaatcggta agaaaagtga tgaaatcttc tttacatctg
    18601 gtgggacaga aggggataac tggatcatca agggagtagc atttgaaaaa gcccagtttg
    18661 gcaagcacat catcgtatca gccattgaac atccagcagt taaggaatca gccctctggt
    18721 tgaaagctca aggttttgaa gtggattttg ctccagttga taacaaagga tttgtggatg
    18781 ttgaggcgct agaagatttg ataagacctg atacgaccct cgtttccatc atggcagtta
    18841 acaatgaaat tggctctgtt cagcccattg aggctatttc aaaactattg gcagacaagc
    18901 caactatttc cttccacgtt gatgcggttc aggcgcttgc caagattccg actgaaaagt
    18961 atctgacaga acgagtggat ttcgcgactt tctctggtca caagttccac ggaatccgtg
    19021 gtgttggttt tatctatatc aagtctggca agaagattac gcctctttta acaggtggtg
    19081 gtcaggaacg tgattaccgt tcgacaactg aaaatgtggc aggaattgca gcgaccgcta
    19141 aggctctccg tttatctatg gaaaagctag atatctttag gagcaagact gggcagatga
    19201 aggcagtgat tcgtcaagca cttctggact atccggatat atttgtcttt tcagatgagg
    19261 aagacttcgc ccctcatatt ctgacttttg gaatcaaggg tgttcgtggt gaggtcattg
    19321 ttcacgcctt tgaagactat gatattttca tctcaacgac ctcggcttgt tcgtccaagg
    19381 ctgggaaacc agccggaacc ctgattgcta tgggagtgga caaggataag gctcagtcag
    19441 ctgtgcgtct tagtctagac cttgaaaatg atatgagtca ggtcgagcag ttcttgacca
    19501 agctaaaatt aatttacaat caaactagaa aagtaagata ggagcattca tgcagtattc
    19561 agaaattatg attcgctacg gtgagctgtc aaccaagggc aaaaatcgta tgcgtttcat
    19621 caataaactt cgcaataata tttcagacgt tttgtctatc tatccccaag ttaaggtaac
    19681 agcagatcgc gaccgtgccc acgcttacct caatggagca gattacacag cagtagcaga
    19741 atcgctcaaa caagtatttg gaattcaaaa cttttcccct gtttataagg ttgaaaaatc
    19801 tgtagaagtt ctgaagtctg ctgtccaaga gattatgcag gacatctaca aggaaggcat
    19861 gacctttaaa atctctagca agcgtagcga ccacaccttt gaattggaca gtcgtgaact
    19921 caaccaaacg cttggaaggg ctgtattcga agccattcca aacgtacaag ctcaaatgaa
    19981 aagtcctgac atcaatcttc aggtggagat acgtgaagaa gcagcctatc tttcttatga
    20041 aaccgttcga ggggcaggtg gattaccagt gggaacttct ggtaagggca tgctcatgtt
    20101 gtcaggggga atcgattccc ctgtagctgg ctatctagcg cttaaacgag gggtagatat
    20161 agaggcagtt cactttgcca gcccacctta cacgagtcct ggtgctctta aaaaagccca
    20221 agatttgacc cgtaaattaa ccaaatttgg gggaaatatc cagtttatcg aagttccttt
    20281 tacagagatt caagaggaaa tcaaagccaa agcgccagaa gcctacctta tgaccttgac
    20341 gcgtcgtttt atgatgcgta ttactgaccg tattcgtgag gtacgaaatg gtttagttat
    20401 catcaatggg gaaagtctag gtcaagtagc cagccaaacc cttgaaagta tgaaggctat
    20461 caatgctgtt accaacactc ccatcattcg tcctgtggtt accatggaca agttggaaat
    20521 cattgacatc gcccaggaaa tcgatacctt tgacatttca atccaaccgt ttgaagactg
    20581 ttgtaccatt tttgcaccag atcgtccaaa aacaaatcct aaaattaaga atgcggagca
    20641 gtacgaagcg cgtatggatg ttgaaggctt ggttgagcga gcagtggctg gaatcatgat
    20701 tactgaaatc acacctcaag ccgaaaaaga tgaagttgat gacttgattg acaatctgct
    20761 ctaattcaga aaatccaaaa gaatagcgaa aatcagtaaa aaaagttagt tttttctcta
    20821 aaaacaggta aaaaactaac tttttttatt tttatgatat aatgatataa aattttgaat
    20881 atagagagtt ttctggcaat gaatcaatcc tacttttatc taaaaatgaa agaacacaaa
    20941 ctcaaggttc cttatacagg taaggagcgc cgtgtacgta ttcttcttcc taaagattat
    21001 gagaaagata cagaccgttc ctatcctgtt gtatactttc atgacgggca aaatgttttt
    21061 aatagcaaag agtctttcat tggacattca tggaagatta tcccagctat caaacgaaat
    21121 ccggatatca gtcgcatgat tgtcgttgct attgacaatg atggtatggg gcggatgaat
    21181 gagtatgcgg cttggaagtt ccaagaatct cctatcccag agcagcagtt tggtggtaag
    21241 ggtgtggagt atgctgagtt tgtcatggag gtggtcaagc cttttatcga tgagacctat
    21301 cgtacaaaag cagactgcca gcatacggct atgattggtt cctcactagg aggcaatata
    21361 cccagtttat cggtttggaa taccaagacc aaattggttg cttgggcgtt ttttcatctg
    21421 caaactggct ccaccaagaa gcctttaacc gctatatcga gtgccagaaa ctatcgcctg
    21481 accagcgcat cttcatctat gtaggaacag aagaagcaga tgatacagac aagaccttga
    21541 tggatggcaa tatcaaacaa gcctatatcg actcgtcgct ttgctattac catgatttga
    21601 tagcaggggg agtacatctg gataatcttg tgctaaaagt tcagtctggt gccatccata
    21661 gtgaaatccc ttggtcagaa aatctaccag attgtctgag attttttgca gaaaaatggt
    21721 aagttaagaa aggaaaaaac gaaatgcata ttgaacatct tagccactgg agtggtcatc
    21781 ttaaccgtga aatgtacctt aaccgttatg gacatggtgg gattccagtt gtggtctttg
    21841 cttcatcagg tggtagtcac aacgaatact atgattttgg catgattgat gcctgtgctt
    21901 cctttatcga ggaaggcctt gtccagttct ttaccctatc tagtttggat agtgagagct
    21961 ggttggttac ttggaaaaat gctcatgacc aagcggaaat gcaccgtgcc tacgaatgtt
    22021 atgtgattga ggaggccatt cttttatcaa gcacaagaca ggttggtttg atggcatgat
    22081 gacgacaggt tgctctatgg gagcctatca tgcactcaat ttcttcctcc agcatccaga
    22141 tgtctttacc aaagtgattg ctctcagtgg tgtttacgac gcacgtttct ttgtcggtga
    22201 ttactacaac gatgatgcta tttaccaaaa ctcgccagta gattatattt ggaaccaaaa
    22261 cgacggctgg tttattgacc gttaccgtca ggcagagatt gtgctgtgta cggggcttgg
    22321 agcctgggaa caagatggtt tgccatcctt ttacaagctc aaagaagcct ttgacaagaa
    22381 acaaattcca gcctggtttg ctgaatgggg acatgatgtc gcccatgact gggaatggtg
    22441 gcgtaaacaa atgccttatt tcctcggtaa tctctattta taaaaggagt tacctatgaa
    22501 ttaccttgtt atttctccct actatccaca aaactttcaa cagtttacca tcgaactagc
    22561 taataaaggc atcacagtct tgggaattgg tcaagagtct tacgagcaat tggatgagcc
    22621 cttgcgcaat agcttgaccg agtattttcg tgttgataat cttgagaaca tagatgaagt
    22681 caaacgtgca gttgcttttc tcttttataa acatggtcca attgaccgca tcgagtctca
    22741 caatgaatac tggcttgagc tagacgcaac actcagagaa caattcaatg tttttggtgc
    22801 caaaccagag gatctcaaaa agacgaaata taagtctgaa atgaagaaac ttttcaaaaa
    22861 agcaggtgtt cctgtggtac ctggagctgt tatcaagacg gaagcagatg ttgatcaagc
    22921 agtgaaagaa atcggtcttc caatgattgc caaacctgat aatggagtgg gagcagccac
    22981 aacctttaaa cttgagacag aagacgatat caatcacttc aagcaagaat gggaccattc
    23041 aaccctttat ttctttgaaa aatttgtcac ttccagcgaa atctgtacct ttgacgggct
    23101 cgtggacaag gatggaaaga ttgtcttctc aacaaccttt gactacgcct atacaccgct
    23161 tgacctcatg atttataaga tggacaattc ttattatgtg ctcaaggata tggatcctaa
    23221 actgcgcaag tatggggaag caattgtcaa agaatttggt atgaaagaac ggtttttcca
    23281 tattgagttc ttccgtgagg gggacgatta tattaccatc gagtacaata accgccctgc
    23341 aggtggtttt accattgatg tttataactt tgctcattcc ttggacctct atcgtggcta
    23401 tgcagctatt gtcgcaggag aggagttccc ggcgtcagac tttgaaactc agtattgttt
    23461 ggctacttct cgccgtgcaa atgctcacta tgtttattca gaagaggatt tgcttgccaa
    23521 atatagccag cagttcaaag ttaaaaaagt catgccagct gccttcgcgg aacttcaagg
    23581 agattacctg tatatgctga ccactccgag tcgacaagaa atggagcaga tgattgcaga
    23641 tttcggacaa cgtcaagaat aagaactatc ggattaagga aattaactcc cttaatcctt
    23701 ttgttttgtc tgataaaaaa taagagcatc ccaacaaggt agctatcata aaacttgttc
    23761 gataactatt tgaagcagga ttaggtggtc agaaattgaa ttttaatatt tcaattgagt
    23821 catagtattg tgtttgcgta tccttaaatc agctaaaagg atccatgacg acacctatac
    23881 gatatagttt tcaagatacc aaacaagtct attaatactc aatgaaaatc aaagagcaaa
    23941 ctaggaagct agccgcaggt ttctcaaaac actgttttga ggttgtggat agaactgaca
    24001 gagtcagtat catataccta cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa
    24061 gagtataaaa tattcaggtg acgcatagat atagttaatt gaagctttgt ttgaaatctg
    24121 ataaaataat gatattacta agttttaaaa actaaagaaa agggaagata tgattacagg
    24181 cgaattaaaa aataaaatcg atcagctgtg ggaaattctt tggacagaag gaaacgcaaa
    24241 tcctttaaca aatattgaac agttgactta tctcttattt atgaaagatt tggatagtgt
    24301 cgagcttgga cgtgaaagtg atgctgaatt tctagggatt ccttatgagg gagtttttcc
    24361 aaaagataaa cctgaatacc gttggtcaac ttttaaaaat ataggagatg ctcaggaagt
    24421 ttatcgttta atgactcagg agatttttcc gtttattaaa aatctcaagg gggatacaga
    24481 tgatacagcc ttttcacgat atatgcgaga agctattttt caaataaata aacctgctac
    24541 gcttcaaaag gcaatttcta tcttagatgt ttttccaact aggggattag atgtagattt
    24601 tgataatgac aaacaaagta ttactgatat cggagatatc tatgaatatc tgttatcaaa
    24661 attgtcgacc gcaggtaaaa atggacagtt ccgtacacct cgtcacatca tcgatatgat
    24721 ggttgagttg atgcaaccga ctatcaaaga tatcatctca gatcccgcta tgggttctgc
    24781 tggcttctta gtatctgcta gccgttactt aaagcgtaag aaagatgaat gggaaaccaa
    24841 tacagataat atcaatcatt ttcataatca gatgtttcat ggaaatgata cggatacgac
    24901 tatgttgaga cttggggcga tgaacatgat gctacatgga gtagaaaatc cacaaatcag
    24961 ttaccttgac tcgctgtctc aagataatga agaagccgat aaatatactt tggttttagc
    25021 aaatcctcct tttaagggct cacttgacta caattcaacc tctaatgacc ttcttgcaac
    25081 cgtaaaaacc aaaaaaacag aattactctt tctttctctt ttcttgcgaa ctttaaaacc
    25141 aggtggacga gcagcagtta tcgtacctga tggtgtcctt tttggttcgt ctaaagctca
    25201 taaaggaatt cgtcaggaaa ttgtagagaa tcataagctt gatgctgtaa tctcaatgcc
    25261 tagtggtgtg ttcaagcctt atgctggagt ttcaactgct attctcatct ttacaaaaac
    25321 tggtaatggt ggtactgaca aagtctggtt ttacgatatg aaagcggatg gtttaagttt
    25381 ggatgataag cgacaaccga ttagcgacaa tgatattcca gatattatcg aacgctttca
    25441 tcatcttgaa aaagaagcag aacgtcagag aacggatcaa tctttctttg ttccagttgc
    25501 tgagataaag gaaaatgatt atgatttgtc tatcaataaa tataaagaga ttgagtatga
    25561 aaaagttgag tatgaaccaa cagaggtcat cttaaagaaa atcaatgatt tagaaaaaga
    25621 aattcaagct ggcttggctg aattagaaaa attactcaag tagggaggtg gctgtatgaa
    25681 aaaagtgaag ttgggggaag tcttatctct aaaaaaagga aagaaagcca ctgtacttgc
    25741 tgaacaaaca actctaagcc aacgttatat tcaaatagat gatttaagaa ataataataa
    25801 tttaaaattc actgaaagtt taaatatgac tgaagcactc ccagatgata ttctaatagc
    25861 atgggatggt gctaatgcag gaacagttgg ctatgggtta tcgggagctg ttggtagtac
    25921 aattacggtc ttaaaaaaga atgagcgata caaagaaaaa attatatcag attacttggg
    25981 agtctttttg gaaagtaaat cgcagtattt acgagatcat tcaacaggtg caactattcc
    26041 tcatttaaac aagaatatat tacttgattt acaattagaa ttgctaggta tcgaagaaca
    26101 agagaacatt atctgtattc ttaatacgat taaaaggctt attactaaaa gaaaatttca
    26161 gttagatgaa ctaaacttgc tcgtcaaatc ccgatttaac gagatgtttg gggatccttt
    26221 aaataataac aagaaatttg cagtaaaaac gggacaacag tgttttaaat tttcaagtgg
    26281 aaaatttctt gataaacatg atagagtttt tgaaggttat cctgcctatg gtggaaatgg
    26341 cattgcttgg aaaagtcgaa aatatttaat tgataatcct actatcatca ttggtcgtgt
    26401 aggtgcatat tgtggtaatg taagaactac ccacggtaag gtatggataa gtgacaatgc
    26461 tatatatata aaagaattta agaattccga tttcaatctt gtatttttat tagaattaat
    26521 gaaagtaatt gattttagta aatttgctga tttctcaggt caaccaaaga taactcaaaa
    26581 accattagaa aatcagaaat atatcctccc ccctctagcc ctccaaaatg agttcgcaga
    26641 ctttgtagcc ctggtcgaca aatcacaatt ggcaatccaa aaatctctgg aagaacttga
    26701 aactttgaag aaatctctga tgcaggagta ttttggctga tattctgcca ttgtaattac
    26761 ggtaatgatt tgttataata cttcaaagga ggaaatcaga tggtagtaaa aacaagaaaa
    26821 caaggaaatt caatcaccat tacgattcca agtgaattta atattccaag tggtgttaaa
    26881 tacgaagcga aattgttacc aagtggtgag attatcttta ctcctgaaga attggggcag
    26941 caggtttctt atgtatctga tgatgccttt gacttaaatt tagataaaat atttgacgaa
    27001 tacgacgatg ttttcaaagc tttggtggaa aaatgacaat ctatctgaca gaaaagcaaa
    27061 ttgaaaaaat aaatgcttta gcaattcaac ggtattctcc aaatgagaaa attcaaacag
    27121 ttagttcttc tgccttaaat atgattgtga acttaccaga acaatttgtc tttgggaagt
    27181 ctctttatcc aacaattttt gataaagcaa cgatactatt tgtccaattg ataaagaagc
    27241 atgtttttgc taatgctaat aaaagaactg ctttcttcgt tttggtcaaa tttttacaat
    27301 taaacggcta tcgtttttct gtaacggtag aagaagcagt aaaaatgtgt gtaaccatcg
    27361 cagtagaagc tttaactgat gaaaaaatga caagctactc caaatgggtt tctgaacatt
    27421 ctgttagaga aaaggtcaaa aagtaaccta gtatgctgga tttgaatgag cacaagaaaa
    27481 taaatgaaca gacaatatta gaattctgta atgcagaaac tgatattgtc tctttttatt
    27541 gatgaataag aaagtgagaa attatggaat caaaagttac aattatcatg caagaaatgt
    27601 tacctctttt aaataatgaa caattactag cgttgagaga gagtttagaa catcatctag
    27661 tagacggaaa aaagcagcag aagtattcga ataataacct gttgcaacta tttattaccg
    27721 ccaagcaggt agagggctgt agctcaaaaa caattcgtta ttaccagagg acgattgaaa
    27781 acttgtttaa tgctattaaa gagtctgtga cacaactcac aacagatgat ttaaggagtt
    27841 atttagcaaa ttaccagtct gaaaaggatt gtagtaaggc aaatttagac aatattaggc
    27901 gtatattgtc ttcttttttt gcttggcttg agcaagagga atatatcatt aaaaatccca
    27961 ttcgacggat aaagaaaatt aagactgagc aaaatgtgaa ggaaacttat actgatgaac
    28021 atttggaaat tatgcgtgat aactgtgaaa atttgagaga tttggcaata atagacctac
    28081 tagcatcgac aggtatgcgt gtaggggagc ttgtacagtt gaatcgttca gatattgatt
    28141 ttgaaaacag agagtgtgtt gtctttggta aaggaaagaa ggagagacca gtatattttg
    28201 acgctcggac gaaaattcat ttaagaaatt atcttaacga cagaaaagat agtcaccctg
    28261 ctctttttgt aacgctagtt ggaaaagccc agagacttgg aattgctggt gtagagattc
    28321 gcttaagaaa gttaggagac aaactcggca tacaaaaggt tcacccacat aagttcagaa
    28381 gaactttagc gactaaggca attgataaag gtatgcctat cgaacaagtc caaaaactgc
    28441 taggtcatag caagattgac acaaccttgg cctatgccat ggtcaatcaa aataatgtca
    28501 agcattcaca ccaaaaattc atctcttaaa agcaaattcc cgatttaacg agatgtttgg
    28561 ggaaaataaa atatttgaaa gcattgataa cttatttgat attatagatg gtgatagggg
    28621 caaaaattat cctaaatcag atgagttgtt tagtgaggag tactgtttat ttttaaatac
    28681 aaagaatgtt actaaaaacg gattttcatt cgatacaaag caatttatca ctaaaacaaa
    28741 ggataaatta cttcgaaaag gcaaacttga gcgttatgat atagtcttga caacaagagg
    28801 tactgttgga aatgtagcgt actacgatga attaata
//
LOCUS       DAVKMU010000023        28459 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKMU010000023 DAVKMU010000000
VERSION     DAVKMU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.023"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            514..1677
                     /locus_tag="VW343_001184"
     CDS             514..1677
                     /locus_tag="VW343_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9755736.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1674..2351
                     /locus_tag="VW343_001185"
     CDS             1674..2351
                     /locus_tag="VW343_001185"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9755737.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2341..3195
                     /locus_tag="VW343_001186"
     CDS             2341..3195
                     /locus_tag="VW343_001186"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9755738.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEAHLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3214..4281
                     /locus_tag="VW343_001187"
     CDS             3214..4281
                     /locus_tag="VW343_001187"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9755739.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4291..5457
                     /gene="aroC"
                     /locus_tag="VW343_001188"
     CDS             4291..5457
                     /gene="aroC"
                     /locus_tag="VW343_001188"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9755740.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5467..6570
                     /locus_tag="VW343_001189"
     CDS             5467..6570
                     /locus_tag="VW343_001189"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9755741.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLESIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            6581..6919
                     /locus_tag="VW343_001190"
     CDS             6581..6919
                     /locus_tag="VW343_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9755742.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            7011..8294
                     /gene="aroA"
                     /locus_tag="VW343_001191"
     CDS             7011..8294
                     /gene="aroA"
                     /locus_tag="VW343_001191"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9755743.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSASFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8287..8763
                     /locus_tag="VW343_001192"
     CDS             8287..8763
                     /locus_tag="VW343_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9755744.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8760..9608
                     /gene="pheA"
                     /locus_tag="VW343_001193"
     CDS             8760..9608
                     /gene="pheA"
                     /locus_tag="VW343_001193"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9755745.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEGVTIPLQSQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9605..10879
                     /gene="psr"
                     /locus_tag="VW343_001194"
     CDS             9605..10879
                     /gene="psr"
                     /locus_tag="VW343_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9755746.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11158..12003
                     /locus_tag="VW343_001195"
     CDS             11158..12003
                     /locus_tag="VW343_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9755747.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11993..13096
                     /locus_tag="VW343_001196"
     CDS             11993..13096
                     /locus_tag="VW343_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9755748.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            13098..14084
                     /locus_tag="VW343_001197"
     CDS             13098..14084
                     /locus_tag="VW343_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9755749.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            14100..15536
                     /locus_tag="VW343_001198"
     CDS             14100..15536
                     /locus_tag="VW343_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9755750.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNNVSLLSTLLFLL
                     VSSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVM
                     VSILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRL
                     FERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSF
                     IAANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAM
                     GVLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPV
                     LDYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYI
                     MELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSE
                     SFFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15565..16599
                     /locus_tag="VW343_001199"
     CDS             15565..16599
                     /locus_tag="VW343_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9755751.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16799..17536
                     /gene="mecA"
                     /locus_tag="VW343_001200"
     CDS             16799..17536
                     /gene="mecA"
                     /locus_tag="VW343_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9755752.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17687..18973
                     /locus_tag="VW343_001201"
     CDS             17687..18973
                     /locus_tag="VW343_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9755753.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18975..19844
                     /locus_tag="VW343_001202"
     CDS             18975..19844
                     /locus_tag="VW343_001202"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9755754.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAAAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19920..19991
                     /locus_tag="VW343_001203"
                     /pseudo
     CDS             19920..19991
                     /locus_tag="VW343_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            20139..21077
                     /gene="msrB"
                     /locus_tag="VW343_001204"
     CDS             20139..21077
                     /gene="msrB"
                     /locus_tag="VW343_001204"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9755755.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21135..22859
                     /locus_tag="VW343_001205"
     CDS             21135..22859
                     /locus_tag="VW343_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755756.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLSKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22861..24609
                     /locus_tag="VW343_001206"
     CDS             22861..24609
                     /locus_tag="VW343_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755757.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLVIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            25001..26260
                     /locus_tag="VW343_001207"
     CDS             25001..26260
                     /locus_tag="VW343_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9755758.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26336..26471
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26510..27010
                     /locus_tag="VW343_001208"
     CDS             26510..27010
                     /locus_tag="VW343_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9755759.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            27086..27454
                     /gene="rplL"
                     /locus_tag="VW343_001209"
     CDS             27086..27454
                     /gene="rplL"
                     /locus_tag="VW343_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9755760.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27701..>28459
                     /locus_tag="VW343_001210"
     CDS             27701..>28459
                     /locus_tag="VW343_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9755761.1"
                     /translation="MSLRNKIEQHIKELEGGKFQKLGDAYLSRKYNFNIVSLGSQEGT
                     DKTTKGIPDSYAVENGKYVYIMYGTHKSVISKLEGDIQSVKKKILEENIAEDKVGRLI
                     CCHTSSNITIKQKEDLEKMAEPYHLELIGINEIANDLTKIDFQYLAKEYLSISESTEQ
                     VWSINDFIRIHDESKTNAPISNDYIGDVSEIINTIKSSEKRIFLISAKPGTGKTRLAI
                     EICSLLDRNKYNIICVKSNNQDIYQDVKRNLNLHK"
BASE COUNT         8512 a         5245 c         6368 g         8334 t
ORIGIN      
        1 ttttcaattt caaaaacgtt aattcctata atgtgtgtga tccaagtatt tttgatagaa
       61 catatattta gagttcgaaa tagaatgatg caaataggtg aaattttaat tattgttgct
      121 tctataatat tatttattga tagtatattg agtttgtaac aaaaggcttt tctataaaat
      181 caaattttcc agatatctac tgaagctgat ttatttcttc atctttcttc tactaccttt
      241 gtcactaaat ttgacagaca catattgctg atacaacctc tctacctata atctcacggt
      301 taacctgagg ctagtttcat agtttgctat ttgattttcg ttgataaaaa gaaatgctcc
      361 tccaagacgg aagagcattt tttactgtga ttcttacaca tttacttcct agttttgcag
      421 gaagtctagt ataccatttt ttaaaagaag agtaaaaaag caagggtagt ttcttttttg
      481 agaattgtct aaaagtttga tataatggta gttatgaata gaataagagt cagcaaaagg
      541 gttgaaaaga agcttgctaa ggggctagtt ttactagaag ccagtgatct tgagaatgtc
      601 aatcttaagg atcaggaagt agaggtgcag ggtcaggaag gaaactttct tgggactgcc
      661 tacctttctc agcaaaacaa gggcttgggc tggtttatca gcaaagacaa ggtggccttc
      721 aatcaagctt tctttgaaac gttgtttaga aaagccaaag aaaagagaaa cgcctactat
      781 caagatgatt tgacaactgc ctttcgtctc tttaatcaag agggagatgg ctttgggggt
      841 ctgacagtgg acctttatgg cgactacgcc gtcttttctt ggtataactc ttatgtttat
      901 cagattcgtc agactatatc agaagccttt agacaggttt tccctgaggt tttaggagct
      961 tatgagaaaa tccgctttaa gggtttggac tatgaatctg cccatgttta tggtcaagaa
     1021 gcacctgact ttttcactgt tttagaaaat ggtgtcctgt atcaagtctt tatgaatgat
     1081 ggcttgatga caggaatttt cctagaccag catgaggttc gcggtagttt agttgacggc
     1141 ttggctatgg gtaaatcctt gctcaatatg ttttcctaca cagcggcttt ttcagtagct
     1201 gcggccatgg gaggagctag ccagacaact tctgttgatc tagccaaacg ttcacgagaa
     1261 ttgtctcaag cgcattttca ggcaaatggg ctcagcacag acgagcatcg ttttatagtc
     1321 atggatgtct ttgagtattt caaatatgcc aaacgcaaag acttgaccta cgatgtgatt
     1381 gtcctagatc cgcctagctt tgctcggaat aaaaaacaaa ctttctctgt ggccaaggat
     1441 tatcacaagt tgatttccca gagtcttgag attttaaatc cgggagggat tatcattgcc
     1501 agtaccaatg ctgccaatgt ttcccgtcag aaatttacag aacaaattga taaaggtttt
     1561 gcaggaagaa gttaccagat tttaaacaaa tatggtcttc cagcagattt tgcctataat
     1621 aaaaaagatg aaagtagtaa ttacctcaag gtgattagta tgaaggttag taaatgaaat
     1681 taatcgtttc agtaatgcca agaagtttag aggaggctca ggctctggat gccacgaggt
     1741 acctggatgc cgacatcatt gaatggcgtg ccgactatct gcctaaagaa gcgattttgc
     1801 aggtagctcc agccattttt gaaaaattcg caggccgtga gttggttttc acgctacgaa
     1861 ctcgctccga aggtggagaa atcgaccttt ctccagaaga gtatatccat ctaatcaagg
     1921 aagttgcgca actctatcaa ccagactata ttgattttga gtactatagc tacaaggatg
     1981 tttttgagga aatgctggac ttcccaaatc ttgttttgag ttaccataat ttccaagaaa
     2041 cacctgagaa catgatggaa atcttgtcag agttgacgat cctaaatcca aaacttgtta
     2101 aggttgcggt gatggctcac acggagcagg atgtcttaga cttgatgaac tatacacgag
     2161 gctttaaaac cctcaatcct gaacaggaat atgtgaccat ttctatgggt aaggtgggca
     2221 aggtctctcg tatcactgcg gatgtgactg gttcgagttg gtcttttgcc agtctggatg
     2281 aggttagtgc ccctgggcag atttctctag ctagcatgaa aaaaatcagg gagattttgg
     2341 atgaagcttg atggctatac acgtttagct gcagttgttg ccaatcctat taagcattct
     2401 atttctccct tcatccacaa tagagccttt gaggcgacag ctaccaacgg tgcttatgtg
     2461 gcttgggaga ttgaagcgag tgacttggca gaaacagtgg ccaatattcg tcgctaccag
     2521 atgtttggca tcaatctgtc catgccctat aaggagcagg tgattcctta tttggataag
     2581 ctgagcgatg aagcgcactt gattggtgcg gttaatacgg ttgtcaatga gaatggcaat
     2641 ttaattggat ataatacaga tggcaaggga ttttttaagt gcttgccttc ttttacaatt
     2701 tcaggtaaaa agatgaccct gctgggtgca ggtggtgcgg ctaaatcaat cttggcacag
     2761 gctattttgg atggcgtcag tcagatttcg gtctttgttc gttccgtttc tatggaaaaa
     2821 acaagacctt acctagacaa gttacaggag cagacaggtt ttaaagtgga tttgtgtgct
     2881 ttagaatatg tttctgaact gcaagcaagg attgccgagt cggatttgct agttaatgcc
     2941 accagtgtgg gcatggatgg ccaatcctct cctgttcctg aaaacatagt cttaccagaa
     3001 actctcttag ttgcagatat catataccaa ccctttgaaa caccgttttt gaaatgggct
     3061 agaagtcagg gcaatccagc agtcaatggt ctgggaatgt tgctctatca agctgcagaa
     3121 gcttttcaac tgtggacagg caaggaaatg ccgacagaag agatttggca gtctttaaca
     3181 gaaaaatacc aataaagaaa gggagactct cctatgaaaa tcagaatcga tattcctcat
     3241 catccttatg atattcagat tgaaaaaggt tgtatggccc aggctggtca gtggttgcga
     3301 gaactctggc aaccgcaaaa ggtagtcatt gtgacagata accatgtagc ctctctctat
     3361 gcagagaagg tcaagctcag cctagaagat gctggttttc aggtagctgt ttttgatttc
     3421 ttagaaggtg aagaaagaaa gaatttaact actgttcaga aagtctatga atttctagtc
     3481 aagcaaggtc tgactcgtag cgatggaatc gttgctcttg gtggtggcgt tgttggggac
     3541 ctggctggat ttgtagcctc tacctatatg cggggtattc actttgttca gattccgact
     3601 agtttgacag ctcaggttga ttcttctatc ggtggaaaga caggcgttaa cacaccattt
     3661 gctaaaaata tggtggggac ctttgcccaa ccagatgggg ttctgattga tccacttgtt
     3721 cttgaaaccc tcggaaaaag agagttgatt gaagggatgg gtgaagttat caagtatggc
     3781 ttgattgagg atccagaact gtgggctctc ttgacgggac tgaatggttc tgttgagagt
     3841 attttggaac atgcagagac cttgattgaa cattcttgtc aggtgaagcg caagatggtg
     3901 gttgaagatg agttggacaa tggtattcgt ctttacctca attttggcca cactattggc
     3961 catgccatcg aagcgactgc cggttatggc aaggtcatgc atggagaggc tgttgccatg
     4021 ggaatggtac agatttccaa ggttgctgag gaaaaaggcc tcatgccagc tggcattacc
     4081 caatctatca cagagatgtg tcagaaattc ggcttgcctg ttgactatga aaattggaaa
     4141 gttgacaagc tttatcaggc tcttactcat gacaagaaag cgcgtggtaa caccttgaaa
     4201 ttggtcttgg tgccagagct tggttcagcg accattcacc cagtttctct ggaagagatg
     4261 aaagactact tggtaaaata aggagagtgt atgagatatt taactgcagg agaatcacac
     4321 ggcccccgtc taacagctat tattgaggga attccagctg gacttccatt gacagctgag
     4381 gatatcaatg aggatcttag acgccgtcag ggtggctacg gtcgtggtgg tcgtatgaag
     4441 attgagagtg accaggttgt ctttacttcg ggtgttcgcc acgggaagac gacaggggcg
     4501 cctattacta tggatgtcat caataaggac caccagaaat ggctggacat catgtctgcg
     4561 gaggacattg aagaccgcct taaaagcaag cggaaaatta ctcatcctcg cccaggtcat
     4621 gccgatttgg ttggggggat taagtaccgt tttgatgatt tgcgaaattc tttggagcgt
     4681 tcatcagctc gtgaaaccac catgcgggtg gcagttggtg cagtagccaa acgcctcttg
     4741 gctgagctgg atatggagat tgccaaccat gtcgtggtct ttggtggtaa ggaaatagat
     4801 gttcctgaaa atctgacagt cgctgaaatt aagcaaagag ctgcccagtc tgaagtttct
     4861 attgtcaacc aagaacgaga acaggaaatc aaggactata ttgaccaaat caaacgtgat
     4921 ggtgatacca tcggtggggt tgtggagaca gtcgtcggag gagttccagt tggtcttggt
     4981 tcctatgtcc aatgggatag aaaattggat gcaagattgg ctcaagctgt tgtctctatc
     5041 aatgccttta aaggggtgga atttggtctt ggctttgagg ctggttatcg taaaggcagc
     5101 caagttatgg atgaaattct ctggtctaaa gaagacggtt atactcgccg taccaataat
     5161 ctaggtggtt ttgaaggtgg tatgactaat gggcaaccca tcgttgttcg tggggtcatg
     5221 aaacccattc ctactcttta taaacctctt atgagtgtgg atatcgaaac ccacgaacct
     5281 tacaaggcaa ccgtggagag aagtgatccg actgctcttc cagctgcagg aatggtcatg
     5341 gaagcagttg tagcaacggt tctggcgcaa gagatcctcg aaaaattctc atcagataat
     5401 cttgaggaac taaaagaagc ggtagccaaa caccgagact atacaaagaa ctattaagga
     5461 gttcctatgg caaaaacaat ctatatcgca ggtcttgggt tgattggagc ctctatggca
     5521 cttggtatca aacgcgatca tccagattat gaaattttag gttataatcg tagtcaagct
     5581 tcgagagata tcgccttgaa agaaggcatg attgaccgtg caacggatga ttttgctagt
     5641 tttgctcctt tggcagatgt cattatcctc agcttgccaa tcaaacaaac tattgctttc
     5701 attaaggagt tggccaattt ggatttgcga gaaggcgtta ttatttcaga tgctggttcg
     5761 accaagtcaa ccattgtgga tgcggcggag cagtatttgg ctggcaagtc tgttcgcttt
     5821 gtcggggccc atcccatggc tggtagtcac aagacagggg ctgcttcggc agatgtcaat
     5881 ctttttgaaa atgcctatta tatctttaca ccttcaagcc tgacaagtca ggacacgctt
     5941 aaggaaatga aggatctgct ttcaggtctt catgctcgtt ttatcgagat tgatgccaag
     6001 gagcatgatc gtgtcacttc tcagattagc cattttcctc atattttggc ttctagtctc
     6061 atggagcaga ctgcggtcta tgctcaagag catgagatgg caaggcgctt tgcggcaggt
     6121 ggttttcgag atatgacccg aattgcggaa agcgagccag gaatgtggac ctccattctc
     6181 ttgtccaata gcgagaccat tctggataga attgaggatt tcaaggaacg tttggaatcg
     6241 attggtcagg ccattagtaa gggagatgaa gagcaaattt ggaacttttt taaccaagcg
     6301 cgtgaacaac gccagaccat ggaaatccat aagcgtggtg gtgtggatag ctcttacgac
     6361 ctctatgttg acgttcctga tgaagaagat gtcatcctgc ggattttgga actgctacgt
     6421 gggacttcct tggttaacat ccacatcaac gaggaaaatc gtgaggatat tcacggaatc
     6481 ctacaaattt catttaaaaa tgctcaagac ttggaaagag ccgagcatct cataacaaaa
     6541 aataccgact atacagtcgt cattaaataa ggagaagaac atgtcaaata tttacgatag
     6601 tgcaaacgaa ctcagtcgcg gtctacgcgg attaccagaa tacaaggctg ttaaagcagc
     6661 taaagatgcg attgcagcag atgctgaggc aagcaaaatc tttacagaat atcttgcctt
     6721 tcaagaggaa attcaaaaac tagcacagac aggtcaaatg ccagatgcgt ccttccaagc
     6781 taagatggaa ggctttggta aacagattca aggaaatagc ctcttgtcag aattctttac
     6841 caagcaacaa caattggcca tttacctttc tgacattgaa aaaattgttt tcgaaccagt
     6901 ttcagaattg ctgaaataag aaaagaacta tcctaaagtc aggaattttt aaggaatttc
     6961 tggcttttta tgataaaata ggtaaaagta ttgcaagtat gaggtccagt atgaaactaa
     7021 aaacaaatat tcgccattta catggtagta tccgcgtccc aggtgacaag tctatcagcc
     7081 accgttccat tatctttgga agtttggctg agggtgagac caaggtttat gatattctgc
     7141 gaggtgaaga cgttctttcg accatgcagg tttttcgtga ccttggtgtt gaaattgagg
     7201 ataaagatgg ggttattacc attcaaggtg taggcatggc tggcttaaaa gcgccgcaaa
     7261 atgcccttaa tatgggaaat tctggcacct cgattcgcct gatttcaggt gtccttgctg
     7321 gtgcagattt cgaagtagag atgtttggag atgatagtct ttccaaacgt cctatggacc
     7381 gtgtgaccct tccactgaaa aaaatgggcg tcagcatctc agggcaaact gaacgagact
     7441 tgcctcccct tcgcttaaaa gggacgaaaa acctaagacc tattcattat gagttgccaa
     7501 ttgcctctgc ccaagtcaag tcagccttga tgtttgcagc cttacaagct aagggggagt
     7561 cagttattat cgaaaaagag tgcacccgta atcatactga agatatgttg caacaatttg
     7621 gtggccattt aagtgtggat ggcaagaaaa tcacagttca aggacctcaa aaactgactg
     7681 gacaaaaggt ggtcgtacca ggagatattt ccagtgcatc cttttggtta gtcgcaggtt
     7741 tgattgtccc aaattctcgt ttagtgctgc agaatgtggg gatcaacgaa actcgcaccg
     7801 gtattattga tgtcattcgt gccatgggtg gaaaattgga aataactgaa atcgatccag
     7861 tcgctaaatc tgctaccttg attgttgagt cttctgactt gaaaggaaca gagattggtg
     7921 gcgctttgat tccacgattg attgatgaat tgcccattat tgccctgctt gcgactcaag
     7981 cccaaggtgt aacagttatc aaggatgctg aggagctcaa ggtcaaggaa acagaccgta
     8041 ttcaggttgt ggcagacgcc ttaaatagta tgggagcaga tattactcct acggcagatg
     8101 ggatgattat caaagggaaa tcagctcttc acggtgctag agtcaatacg tttggtgacc
     8161 accgtatcgg catgatgaca gctatcgcag ccctcttggt tgcagatgga gaggtggagc
     8221 ttgaccgtgc agaagccatc aataccagct atcctagttt ctttgatgat ttggagagct
     8281 tgattcatgg ctaaggtgtt attagggttt atgggggctg gaaaatcgac tattgcaaga
     8341 ggcttggacc ctaattacct tgatatggat gctctgattg agaagcgcct aggtatgtcc
     8401 attgcgaatt ttttcgctga aaagggagaa gcagcctttc gtcaggtaga atcagaagtc
     8461 ctagctgatt tactacaaac agaccaagtc gtgtcaactg gaggaggagt ggttatttct
     8521 cagagaaatc gtgacttact caagactaat acagataaca tctacctgaa agcagatttt
     8581 gaaaccctct acctacgtat cgcagctgat aaggacaatc agcgaccgct ttttctaaat
     8641 aatagcaagg aagaactagt agctattttt caagaaagac aggcttggta tgaggaagtg
     8701 gctagtcggg ttttggatgt gaccaagcta agcccagagg aaattataga ggaactaaga
     8761 tgaaaatagc ttatctaggt cccaagggat cattttcaca ccacgttgtg cagacagctt
     8821 ttcctcatga ggaattgcag gcttttgcca acattacaga tgtcatcaag gcttatgagc
     8881 aaggattagt gaactattct gtggtgccag ttgaaaattc tattgagggt agtgttcatg
     8941 aaaccttgga ctatcttttt catcaggctc acatccaagc agtagcagaa atcgttcagc
     9001 ctattcatca gcagttgatg gtggttccag gccatactaa gattgaaaag attttttcac
     9061 atccacaggc cttggctcaa ggaaagaaat tcatcgatga acaatatcca gaggctcaaa
     9121 tcgaggtaac agctagtaca gcttatgcgg cccgttttat ttccgaacat ccagatcagc
     9181 cctttgcagc agttgcacct agaagttctg ctgaagaata tggattggaa ctgattgccg
     9241 aggatattca ggaaatggaa gccaatttca cacgtttttg ggttttggga gctgaaggtg
     9301 taactattcc cttgcaatca caaactgaaa aaatgagttt agccttgacc ctacctgaca
     9361 accttccagg tgcactttat aaagccctgt cgacctttgc ttggcgagga attgacttga
     9421 caaaaattga aagtcgtcca ctcaagacag cactgggtga atactttttc attatcgatg
     9481 tggattatac cgataaggac ttggtccact ttgcccaaaa agaattagaa gcgattggaa
     9541 tccagtataa aattctgggt gcctatccta tttatccaat atcagaccat ggaaaggaga
     9601 gaagatgagt aaagaaaatc ccttaagtca tcatgagcag ttgcgttatg attatttgct
     9661 aaaaaatatt cactatctca atgagagaga aaaaaatgag tttgcctatt tgcaagaaaa
     9721 gctaactctt gctaggggaa atagtagctc tagcttggaa caagaaagag aagagcaggt
     9781 tgacttacca agctatgcga accggagtcg ctcacaatcc aaatcacaag cactctcttt
     9841 ccctccaaaa aagaaaagac ggaagctccg tcttaaacga atttttatgg tgattttttc
     9901 acttttggtc tgtgtggctt tggccatggt attcatgttt ttgcgtggtt accaagatgc
     9961 tagcgcaaag aaaactgctg atgcccgggc agctcaagta gaagtattta atggtcagga
    10021 cactagagat ggagttaata ttttaatcat gggtactgat ggtcgaatcg gccagaacag
    10081 tgttgagaca cgaactgact ctattatggt attaaatgtc gggggctcag ataagaaaat
    10141 gaagctagtc agtttcatgc gtgataattt ggtctatata gatggttata gtcaagtgat
    10201 taatggtaga aaacagacag ataacaagtt aaacgtagcc tacgagttag gagaacaaga
    10261 ggggcaaaaa ggggcagaaa tggttcgcca agtcttgaaa gataattttg acttggacat
    10321 taagtactac gccttggttg atttccaggc ctttgccaca gcgattgaca cgcttttccc
    10381 tgatggggtg acgattgatg ctcaattttc aacattgaat gggcgtcccc taacagaagc
    10441 tacagtcgga gatgatttat atgctaccga gactgagtct ccaacccaaa ccattaaagt
    10501 cggaaaacag cagatgaatg gctcgaccct gctcaattat gctcgtttcc gtgatgatga
    10561 tgaggcggat tacggccgta caaaaagaca gcaacaagtt ttaacagcaa ttttagagca
    10621 aattaaagat cccactaaac ttttcactgg ttctgaagct cttggaaagg tttttgctat
    10681 gacctcaacc aacgtaccct atactttcct cctaacaaac ggtttatctg ttttggatgg
    10741 agcaaaaaat ggtattgaaa aattgacgat tccagaactg ggtgactggg tagatgccta
    10801 tgatgtttat ggaggcttgg gcttgctggt tgatcaaaac aaatatcaaa ccaagctcgc
    10861 tcaaatgggc ttgagataag ataatactca atgaaaatta aagtgcaaac taggaagcta
    10921 gccgcaggct gctaaaaaca cagttttgag gttgtagata agactgacga agtcagttac
    10981 ctatacctac ggcaaggtga agctgacgtg gtttgaagag attttcgaag agtataagac
    11041 agaaaaatca aagtttgata gctatttgga tagcagtcag gctttgattt tttacagtct
    11101 gcaatagatt ttcacccctt gtttgtggta taatgaaacg atacgataga aagaataatg
    11161 aacaatatga ctgatttaaa agcaattcag gctcgtagtc tggagatggc tgaatatttt
    11221 gtggcctttt gtaaagaaca tgatttgctc tgttatctct gtggcggagg cgctattggt
    11281 acccttcgaa acaagggatt tattccttgg gacgacgacc tagacttttt tatgcctcgt
    11341 aaagattatg agaaattagc agaattatgg cctcgttatg cagatgaacg ttatttcttg
    11401 tcaaagagtt acaaggattt tgttgatcgt aatcttttta ttaccattcg tgacaaggaa
    11461 accacctgta tcaagcctta tcagcaggat ttggatttgc cacatggtct ggccttggat
    11521 gttttgcctt tggattatta tccgaaagat ccagctgagc ggaaaaaaca ggttcgttgg
    11581 gccttgattt attcactctt ttgtgcgcaa actattccag aaaagcatgg tgctctcatg
    11641 aaatggggaa gtcgcatttt actgggtttg actccaaaat ctctccgtta tcgcatctgg
    11701 aaaaaagctg agaaagaaat gactaagtat gatttggctg attgtgatgg cattacagaa
    11761 ttatgctcag gtcctggcta catgagaaac aagtacccaa tcacatcttt tgaagacaat
    11821 cttttcttgc catttgaagg aacagagatg cctattccaa tcggctatga tgtctatctc
    11881 agaactgctt ttggggatta tatgacgcct ccaccagcag acaagcaggt accgcatcat
    11941 gatgctgtca tcgctgatat ggataagtct tatacagaat acaagggaga atatggtggc
    12001 taagaaaaaa atcttatttt ttatgtggtc tttttctctt ggaggtggtg cagagaagat
    12061 tctatcaacc attgtttcaa atctggatct agaaaagtat gatattgata ttcttgaaat
    12121 ggagcacttt gacaagggat atgaatctgt tccaaagcat gtacgcattt taaaatccct
    12181 tcaagattat cgccaaacca gatggttacg agcttttttg tggagaatga gaatttattt
    12241 tccaagactg gctcgtcgtt tgcttgtaaa agatgattat gatgttgaag tttcttttac
    12301 cattatgaat ccaccactgt tgttctctaa aagaagagaa gtcaagaaga tatcttggat
    12361 tcatggaagt attgaagaac ttcttaagga tagctctaaa agagaatcac atagaagcca
    12421 gttggatgct gcgaatacaa ttgtagggat ttcaaaaaag accagcaatt ctatcaagga
    12481 agtttatcca gattatgctt ctaaattaca gacaatctac aatggatatg attttcagac
    12541 tattctagaa aaatctcaag agaagatcga tatcgagatt gctcctcaaa gtatctgtac
    12601 tatcggacgg attgaggaaa ataagggttc tgaccgtgta gtggaagtga tacgattatt
    12661 acaccaagag ggaaaaaact atcatctcta ttttatcggg gctggtgata tggaagagga
    12721 actgaaaaaa cgagtcaaag agtatgggat tgaggactat gtacatttcc ttggttatca
    12781 aaaaaatcct tatcagtatc tatctcagac gaaagttctc ttgtctatgt ctaaacaaga
    12841 aggttttcct ggagtgtatg tggaggcctt gagtctggga ctccctttta tctctacgga
    12901 cgttggaggg gctgaggaat tatcccaaga aggacgattt ggacaaatca ttgagagcaa
    12961 tcaagaggca gctcaggcga ttactaatta catgacttct gcctcaaact ttgatgtcga
    13021 tgaggctagc caattcattc aacaatttac aattacaaaa caaatcgaac aagtagaaaa
    13081 actattagag gagtagcatg gaaactgcat taattagtgt gattgtgcca gtctataatg
    13141 tggcgcagta cctagaaaaa tcgatagctt ccattcagaa gcagacctat caaaatctgg
    13201 aaattattct tgttgatgat ggtgcaacag atgaaagtgg tcgcttgtgt gattcaatcg
    13261 ctgaacaaga tgacagggtg tcagtgcttc ataaaaagaa cgaaggattg tcgcaagcac
    13321 gaaatgatgg gatgaagcag gctcacgggg attatctgat ttttattgac tcagatgatt
    13381 atatccatcc agaaatgatt cagagcttat atgagcaatt agttcaagaa gatgcggatg
    13441 tttcaagctg tggtgtcatg aatgtctatg ctaatgatga aagcccacag tcagccaatc
    13501 aggatgacta ttttgtctgt gattctcaaa catttctaaa ggaatacctc ataggtgaaa
    13561 aaatacctgg gacgatttgc aataagctaa tcaagagaca gattgcaact gccctatcct
    13621 ttcctaaggg gttgatttac gaagatgcct attaccattt tgatttaatc aagttggcca
    13681 agaagtatgt ggttaatact aaaccctatt attactattt ccatagaggg gatagtatta
    13741 cgaccaaacc ctatgcagag aaggatttag cctatattga tatctaccaa aagttttata
    13801 atgaagttgt gaaaaactat cctgacttga aagaggtcgc ttttttcaga ttggcctatg
    13861 cccacttctt tattctggat aagatgttgc tagatgatca gtataaacag tttgaagcct
    13921 attctcagat tcatcgtttt ttaaaaggcc atgcctttgc tatttctagg aatccaattt
    13981 tccgtaaggg gagaagaatt agtgctttgg ccctattcat aaatatttcc ttatatcgat
    14041 tcttattact gaaaaatatt gaaaaatcta aaaaattaca ttagaacggg ggtgaccaag
    14101 tgattgatgg gaaacgatta ttatttagtt tgaccatagt cagttatgcc ttgacgctag
    14161 taagtggaat tgtgtatctg tttaataata ataataatgt tagcttactt tctactttat
    14221 tgttcttgtt ggttagtagc ttaattgctt gttggaatga tatcaagtat tacttaatcc
    14281 attttatttt ctatttaacc atttttgtat ttctggtatc aagaccgacc attgattatt
    14341 ttagggatgg tgctttggat acctatcatc caatagccta tcgttttgcc tttatagttg
    14401 tcatggtttc gattctgggc ttgaccacag gaggcattct ggctcgttac ttcatagcta
    14461 ggaagaaaat aaaagtagca aatataggaa attctctaaa agaggtttat atcaagcggt
    14521 tacgctttgt atcactagga gtttttcttc taacctatcc tttctatttc attcggttat
    14581 ttgaacggct cttgtatcgt ttgcagactt cctactatgc ctactatgca aattttgaaa
    14641 gtaaactgcc ttattttacc tacattttgt ctacctttac ggtctatgca atgtgtatgt
    14701 atctggcaac caagccaaag aaattgcagg ccacagcagt gcttgtctcc tttattgcag
    14761 ctaatactat tcatttggca attgggacac gaaatccctt tattttaagt attttatttg
    14821 cttttgttta ttactttatg cgggagcaaa ctgaaaaagg aaaatggatt gggtttaaag
    14881 aaaagttagc gatttttgta ggttctccta ttctcatgtt agcgatggga gtactcaatt
    14941 atgtacggga taatgtccaa gtttctcata caggtttctg ggatatctta cttgacttta
    15001 tctataaaca agggactagt tttggtgttc tggctcgagg ttttctattt aacagtagcc
    15061 tcccttaccg agatttccgt aattttactt ttggtcctgt tcttgattat tttgcaaggg
    15121 ggagtttggg agccattttc ggaggaaaag cctttgaaca tacaaccaat agtgtggaac
    15181 tagctattga tagtaatagt tatgcccaca atctatccta tcttgtcttg aacaaggaat
    15241 acttgaaagg gcatggtatc ggaagtagtt atatcatgga gttgtatacc gactatggta
    15301 tgattggagt ctttctgctt agtttcttac tcggcgtatt atttatagcc atgctgcaag
    15361 tagcctatcg ctcaaggaca atcctatttg ctttatccct actcatcttg aataatctat
    15421 tctttatgcc aagaagcagc ttttcagaaa gtttcttcaa tttatttaca atgcaattct
    15481 ggggaattgt tcttgtgatt atatttgtag caaaaatgct tacaaaggaa aactagtatc
    15541 tactaaacaa aggagaaaaa aatcatgttt gaacattatt cagtagctga tttgtttgca
    15601 aatctttata aaaaacgaaa agcaaatatt ttagctctca tcgctttatt tgctctcatt
    15661 gctgtaccat ttacaattaa agcagttagg aataaaaaca ctgtcaaaga tacaacaagt
    15721 tattcaactt atcttatcta taaaatcact cctccagaag attcggccaa aacgattttg
    15781 aatcatcaaa ttggtggtta tagtgatttt tatgggaaat tgattgatgg taatttgaat
    15841 ggagcttatc ttttcaatga tgtagaaccc agtgagttga aaaaaattgc cagtgaatta
    15901 gacacgacgg aaacaacctt gaaaaattca acgaatgact attggtggaa aaaattgacc
    15961 gtctactata tgattgacga tgcaggggtt ggtgtgaaaa ttttgacatc aagtaaagat
    16021 gccaatgact tgttagagaa aaaaattgat gggttgattg agaaatttaa acatgcttat
    16081 gcaaatgtga aaattgaaaa actggaaacc atcaactcta aagaattgaa cgcaaatggt
    16141 gaaacagcgc ttggcttaaa tgtgaaaaat ctgattcttc gtttagttgt tattggagtg
    16201 gtttgtgtga ttttggttgt gatgggaaat gtgttagttt atctctttaa tccaacaatc
    16261 aatagagtag gtgatttttc tcagtatcaa attgattttg taacagagat tacaacaatt
    16321 gctaacctag cagatgtttt gtcatacaaa aatactggac aggaattgac catcgttagc
    16381 tcaaataaag ctatcctaga taaattgaaa cagagtcaag aagctttaaa aggaatgcat
    16441 tttgtagatt tgcaggatgt atcatctctt ttggaaagag atacagtcct tcttgttgaa
    16501 gagtacggag tgactcgtta taagaaattt gagcaaagtc ttcaaattct cagaaactta
    16561 aatcgttcta tcctcggtgt agcaaccttt aaattataag tattctgatg tataaggtct
    16621 tcaaaatcgg ctaagagccg attttgaagg ctttttctat ctgtctgcag aaattttctc
    16681 ttggagtctc ctatagaaag accctaggga aaatctatcc gtatagagga gccttctttt
    16741 gataaaatcg taaaaatcta gtataataga tagaactgaa agtatgaggt tactagcaat
    16801 gaaaatgaaa caaattagtg atacaacttt aaaaatcacg atgtctttag aggatttgat
    16861 ggatcgtgga atggagattg ctgactttct cgttcctcaa gaaaaaacag aagagttctt
    16921 ttatgctatc ttggatgagc tagagatgcc tgatagcttt ctggatacag gcatgttgag
    16981 cttccgtgtg actccaaaac ctgataaggt agatgtcttt gtgaccaagt caaagattga
    17041 ccaaaatcta gattttgaag acttatcgga tttgccagat atggaagaat tggctcaaat
    17101 gtctccagat gaatttatca aaaccctgga aaaaagcatc gcagacaaaa ccaaggatga
    17161 tatcgaagcc attcaatctc ttgagcaagt tgaagccaag gaagaagagc aagagcaggc
    17221 tgaacaagaa gctgagagta agaaagaacc ttacatctac tacatccttt cttttgctaa
    17281 gttggctgac ttggtagctt ttgccaagac agtgactttt gaaatggaaa cttctgaact
    17341 ctacaaaatg aacgagcgct attatttgac cattttagtg gatattgaaa atcatccaag
    17401 cccatatcca gcttggctgt tggcccgtat gcgcgagttt gcagacgata gtgatatcag
    17461 tcgctcagtc ttacaagagt atggtcaagt cttgatgagt cacgatgcag tgctcaatct
    17521 gcaaaaaatc ggctaatcct ttctggaaag ctattttcag attttaagaa gagcgaatcg
    17581 tctgattcgt tttttctttt tatactgaaa tagtgattta ctataatagg aattttcaca
    17641 aaattctgtt ataatggcta tattagaaaa tttcgaggag ataaagatga cagttaaaat
    17701 tgctttacta ggatttggta ccgttgcaag tggtgtgcct ttcctcctaa aggaaaatgg
    17761 aggaaaaatc aatcaatcag cacattcaga tatcaaagtt gctaaggtat tggtcaagga
    17821 tgaagatgaa aaaaatcgct tgcttgcagc agggaatgac tttaactttg taaccaatgt
    17881 ggatgatatt ttatcagacc aggatattac tatcgtagtg gaattgatgg ggcgtattga
    17941 gcctgctaaa acctttatca ctcgtgcctt ggaagctgga aaacacgttg ttactgctaa
    18001 caaggacctt ttagctgtcc atggcgcaga attgctagaa atcgctcaag ctaacaaggt
    18061 agcactttac tacgaagcag cagttgctgg tgggattcca attcttcgta ctttagcaaa
    18121 ttccttggct tctgataaaa ttacgcgcgt gcttggagta gtcaacggaa cttccaactt
    18181 catggtgacc aagatggtgg aagaaggctg gtcttacgat gatgctcttg cggaagcaca
    18241 acgtctagga tttgcagaaa gcgatccgac gaatgacgta gatgggattg atgcagccta
    18301 caagatggtt attttgagcc aatttgcctt tggcatgaag attgcctttg atgatgtagc
    18361 ccacaaggga atccgcaata tcacaccaga agacgtagct gtagctcaag agcttggtta
    18421 cgtagtgaaa ttggttggtt ctattgagga aacttcttca ggtattgctg cagaagtgac
    18481 tccaaccttc ctaccgaaag cgcacccact tgctagtgtg aatggcgtaa tgaacgctgt
    18541 ctttgtagaa tctatcggta ttggtgagtc tatgtactac ggaccaggtg cgggtcaaaa
    18601 accaactgca acaagtgttg tagctgatat tgtccgtatc gttcgtcgtt tgaatgatgg
    18661 taccattggc aaagacttca acgaatatag ccgtgacttg gtcttggcaa atcctgaaga
    18721 tgtcaaagca aactactatt tctcaatctt ggctccagac tcaaaaggtc aggtcttgaa
    18781 gttggctgaa atcttcaatg ctcaagatat ttcctttaag caaatccttc aagatggcaa
    18841 agagggtgac aaggcgcgtg tcgttatcat cacacacaag attaataaag cccagcttga
    18901 aaatgtctca gctgaattga agaaggtttc agaattcgac ctcttgaata ccttcaaggt
    18961 gctaggagaa taagatgaag attattgtac ctgcaaccag tgccaatatc gggccaggtt
    19021 ttgactcggt cggtgtagct gtaaccaagt atcttcaaat tgaggtctgc gaagaacgag
    19081 atgagtggct gattgaacac cagattggca aatggattcc acatgatgag cgtaatctct
    19141 tgctcaaaat cgctttgcaa attgtaccag acttgcaacc aagacgcttg aaaatgacca
    19201 gtgatgtccc tttggcgcgc ggtttgggtt cttccagctc ggttatcgtt gctgggattg
    19261 aactagccaa ccaactgggt caactcaact tatcagacca tgaaaaattg cagttagcga
    19321 ccaagattga agggcatcct gacaatgtgg ctccagccat ttatggtaat ctcgttattg
    19381 caagttctgt tgaagggcaa gtctctgcta tcgtagcaga ctttccagag tgtgattttc
    19441 tagcttacat tccaaactat gaattacgta ctcgcgacag ccgtagtgtc ttgcctaaaa
    19501 aattgtctta taaggaagct gctgctgcaa gttctatcgc caatgtagcg gttgctgcct
    19561 tgttggcagg agacatggtg accgctgggc aagcaatcga gggagacctc ttccatgagc
    19621 gctatcgtca ggacttggta agagaatttg cgatgattaa gcaagtgacc aaagaaaatg
    19681 gggcctatgc aacctacctt tctggtgctg ggccgacagt tatggttctg gcttctcatg
    19741 acaagatgcc aacaattaag gcagaattgg aaaagcaacc tttcaaagga aaactgcatg
    19801 acttgagagt tgatacccaa ggtgtccgtg tagaagcaaa ataaagaata gaagatagga
    19861 tggggaaact cttgaccaga gggtttcata tcctttttgt gaaaagaagt ttatactcaa
    19921 tgaaaatcaa agagcaaact aggaagctag ccgcaggctg ctcaaaacag tgttttgagg
    19981 ttgtggatag aactgaagaa gtcagctcaa gacactgttt tgaggttgca gatagaactg
    20041 acgaagtcag taacatatat acggtaaggt gacgctgacg tggtttgaag agattttcga
    20101 agagtattag ttaaaaacgt gataaaggag aaataaagat ggcagaaatt tatctagcag
    20161 gtggttgttt ttggggccta gaggaatatt tttcacgcat ttctggagtg ctagaaacca
    20221 gtgttggcta cgctaatggt caagtcgaaa cgaccaatta ccagttgatc aaggaaacag
    20281 accatgcaga aacggtccaa gtgatttacg atgagaagga agtgtcactc agagagattt
    20341 tactttatta tttccgagtt atcgatcctc tatctatcaa tcaacaaggg aatgaccgtg
    20401 gtcgccaata tcgaactggg atttattatc aggatgaagc agatttgcca gctatctaca
    20461 cagtggtaca ggagcaggaa cgcatgctgg gtcgaaagat tgcagtagaa gtggagcaat
    20521 tacgccacta cattctggct gaagactacc accaagacta tctcaggaag aatccttcag
    20581 gttactgtca tatcgatgtg accgatgctg ataagccatt gattgatgca gcaaactatg
    20641 aaaagcctag tcaagaggtg ttgaaggcca gtctatctga agagtcttat cgtgtcacac
    20701 aagaagctgc tacagaggct ccatttacca atgcctatga ccaaaccttt gaagagggga
    20761 tttatgtaga tattacgaca ggtgagccac tcttttttgc caaggataag tttgcttcag
    20821 gttgtggttg gccaagtttt agccgtccga tttccaaaga gttgattcat tattacaagg
    20881 atctgagcca tggaatggag cgaattgaag ttcgttctcg ttcaggcagt gctcacttgg
    20941 gtcatgtttt cacagatgga ccgcgggagt taggcggcct ccgttactgt atcaattctg
    21001 cttctttacg ctttgtggcc aaggatgaga tggaaaaagc aggatatggc tatctattgc
    21061 cttacttaaa caaataaaac agagagtggg gcttcccact ttcttcattt ctagaatatg
    21121 aatagaaggg atttatgaaa cacctattat cttacttcaa accctacatc aaggaatcaa
    21181 ttttagcccc cttgttcaag ctgttagaag ctgtttttga gctcttggtt cccatggtga
    21241 ttgctgggat tgttgaccaa tctttacctc agggagatca aggtcatctc tggatgcaga
    21301 ttggcctgct ccttatcttt gcagtaattg gcgttttagt ggccttgata gctcaatttt
    21361 actcagcaaa ggcagcagta ggctttgcta aggaattgac aaacgatctt tatcgtcata
    21421 ttctttcctt gtccaaggac agcagagacc gtctgacaac ttctagtttg gtcactcgct
    21481 tgacttcgga tacctaccag attcagactg gtatcaatca attcctgcgt ctctttttgc
    21541 gagcgcccat tatcgttttt ggtgccattt ttatggctta tcgaatctca gctgagttga
    21601 ctttctggtt cttagtcatg gttgccattt tgaccattgt cattgtaggg ctatctcgat
    21661 tggtcaatcc tctctacagt agtctcagaa agaaaacgga ccaactggtt caggaaacgc
    21721 gccagcaatt gcaagggatg cgggttattc gtgcttttgg tcaagaaaaa cgagagttac
    21781 agatttttca aacccttaac caagtttatg ctagattaca agaaaagaca ggtttctggt
    21841 ctagtttatt aacacctctg acctatctga ttgtcaatgg aactcttctc gttattatct
    21901 ggcaaggcta tatttcaatt caaggaggag tgctcagtca aggtgctctc attgctctta
    21961 tcaattacct cttacagatt ttggtggaat tggtcaagct tgccatgctg atcaattccc
    22021 tcaaccagtc ctatatctca gccaagagaa tcgaggaggt ctttgcggaa tctcccgaaa
    22081 acatccattc agaattagaa caaaagcaag ttaccagtgg tcgggtttta caagtccaag
    22141 aattgacttt tacctatcct gatgcggccc agccttctct gagagacatt tcctttgata
    22201 tgactcaagg acaaatcctt ggtatcattg gggggactgg ttctggtaaa tcaagcttgg
    22261 tgcaactctt acttggactt tatccagtag acaaggggaa cattgacctt tatcaaaatg
    22321 gacgtagtcc tcttaatttg gagcagtggc ggtcttggat tgcctatgta cctcaaaagg
    22381 tcgaactctt taaaggaacc attcgttcca acttgactct aggtttcaat caagaagtat
    22441 ctgaccagaa actctggcag gccttggaga ttgcgcaagc taaggatttt gtcagtgata
    22501 aggaaggact tttggatgcc ctagttgagg caggggggcg aaatttctca ggtggacaaa
    22561 aacaaaggtt gtctatcgcc cgagcagtct tgcgccaagc tccgtttatc atcctagatg
    22621 atgcaacctc ggcactggat accattacag agtccaagct cttgaaagct attagagaaa
    22681 attttccaaa cacgagctta attttgatct ctcaacgaac ctcaacttta cagatggcgg
    22741 accagattct cctcttggaa aaaggtgagt tgctagctgt tggcaagcac gatgacttga
    22801 tgaaatccag ccaagtctat cgtgaaatca atgcatccca acatggaaag gaggactaga
    22861 atgaaacgac aaactgtaaa ccagacgctc aaacgtttag ccgtagattt agcaaaccat
    22921 cccttcctcc ttttcctagc ctttctagga actattgccc aagttggctt atcaatttac
    22981 ctacctattc tgattgggca ggtcattgac caagtcctag tggctggttc atcaccagtt
    23041 ttttggcaga tttttctcca gatgctcttg gtggtaatag gaaatactct ggtacaatgg
    23101 gccaatcctc tcctctataa tcgtctaatc ttctcttata ctagagattt gcgagagcga
    23161 atcatccata agctccatcg tttaccgatt gcttttgtag ataggcaagg tagtggagag
    23221 atggttagtc gtgtaaccac ggacatcgaa cagttggcag ctggcttgac catgattttt
    23281 aaccaatttt tcattggtgt tttgatgatt ttggttagta ttctagccat gctccaaatt
    23341 catctcctca tgactctctt agtcttgctg ttgacgccac tgtccatggt gatttcacgc
    23401 tttattgcca agagatccta tcatctcttc cagaagcaaa cagagacgag gggaattcag
    23461 actcagttga ttgaagaatc gcttagccag cagactataa tccagtcctt caatgctcaa
    23521 acagaattta tccaaagatt gcgtgaggct catgacaact actcaggcta ttctcagtca
    23581 gccatctttt attcttcaac ggtcaatcct tcgactcgct ttgtaaatgc actcatttat
    23641 gcccttttag ctggagtagg agcttatcgt atcatgatgg gttcaacctt gaccgtcggt
    23701 cgtttagtga cttttttgaa ctatgttcag caatacacca agccctttaa tgatatttct
    23761 tcagtgctag ctgagttgca aagtgctctg gcttgcgtag agcgtatcta tggagtctta
    23821 gatagccctg aagtggctga aacaggtaag gaagtcttga ccagtgacca agttaaggga
    23881 gctatttcct ttaaacatgt ctcttttggc taccatcctg aaaaaatttt gattaaggac
    23941 ttgtctatcg atattccagc tggtagtaag atagccatcg ttggtccgac aggtgctgga
    24001 aaatcaactc ttatcaatct ccttatgcgt ttttatccca ttagctcggg agatatcttg
    24061 ctggatgggc aatccattta tgattataca cgagtatcat tgagacagca gtttggtatg
    24121 gtgcttcaag aaacctggct cacacaaggg accattcatg ataatattgc ctttggcaat
    24181 cctgaagcca gtcgagagca agtaattgct gctgccaaag cagctaatgc agactttttc
    24241 atccaacagt tgccacaggg atacgatacc aagttggaaa atgctggaga atctctctct
    24301 gtcggccaag ctcagctctt gaccatagcc cgagtctttc tggttattcc aaagattctt
    24361 atcttagacg aggcaacttc ttccattgat acacggacag aagtgctggt acaggatgcc
    24421 tttgcaaaac tcatgaaggg ccgcacaagc ttcatcattg ctcaccgttt gtcaaccatt
    24481 caggatgcgg atttaattct tgtcttagta gatggtgata ttgttgaata tggtaaccat
    24541 caagaactca tggatagaaa gggtaagtat taccaaatgc aaaaagctgc ggcttttagt
    24601 tctgaataag ccattctctt ttgaaagttt atggacgaaa aaagttgcct tcgagtgact
    24661 tttttgttac aatagctaga aaaattgttc actgtaatac tcaatgaaaa tcaaagagca
    24721 aactaggaag ctagccgtag gttgctcaaa gcacagcttt gaggttgtag ataagactga
    24781 cgaagtcagc tcaaaacact gttttgaggt tgcagataga actgacgaag tcagctcaaa
    24841 acactgtttt gaggttgcag atagaactga cgaagtcagc tcaaaacact gttttgaggt
    24901 tgcagataga actgacgaag tcagtaacca cacctacggc aaagtgaatc tgaagtggtt
    24961 tgaagagagt acaacttgtc ttttagaaaa ggagcctata atgaaagtct ttcagcatgt
    25021 aaatatcgtg acttgtgatc aaaatttcca tgtttatctt gatggaatct tagcagtcaa
    25081 ggattctcaa atcgtctatg tcggtcaaga taagccagcg tttttagaac aagctgagca
    25141 gattatagac tatcagggag cttggattat gcctggtttg gtcaattgtc acacccattc
    25201 tgcaatgaca ggtctgagag ggatccgaga tgacagcaat ctccatgaat ggctcaatga
    25261 ctatatctgg ccagcagaat ctgagtttac tcccgacatg actaccaatg cggttaaaga
    25321 agccctaaca gagatgctcc agtcaggaac aacaaccttt aacgatatgt ataatcccaa
    25381 tggtgtggat atccagcaaa tttatcaggt ggtgaaaact tccaagatgc gttgttattt
    25441 ttctccgact ctcttttctt cagagacaga aacaactgct gagactataa gcagaactcg
    25501 atccatcata gatgaaatct taaaatataa aaatccaaat ttcaaggtta tggtagcacc
    25561 tcattctccg tatagctgca gtagagactt gctggaagcg agtttggaaa tggcaaaaga
    25621 gctaaatatt ccgctccatg tccatgtggc ggagaccaag gaggagtcag gaattatcct
    25681 caaacgatac ggcaaacgcc cccttgcttt tcttgaagaa ctgggttatt tagatcatcc
    25741 gtccgtattt gctcacgggg tcgaattaaa cgagagagaa attgaacgct tggcatcttc
    25801 tcaagtggct atcgcccaca atcctatcag taacctcaaa ctggcatcag gaattgctcc
    25861 aattatccag ctccaaaaag cgggagtagc agtcggaatt gcgactgact cggttgcttc
    25921 caataacaat ctagatatgt ttgaggaagg aaggactgca gctcttcttc agaagatgaa
    25981 aagtggggat gccagccagt ttccaatcga aacagctctc aaggtactga caatcgaagg
    26041 ggctaaggtc cttggaatgg aaaatcagat aggaagtctg gaagtcggca agcaagcaga
    26101 ttttctggtc attcaaccac aagggaaaat tcatctccaa cctcaggaaa atatgctgtc
    26161 tcacctggtt tatgcagtta aatctagtga tgtagatgat gtttatatcg ccggagaaca
    26221 ggttgttaag caaggtcaag tcctgacagt agaactttaa aagaaaaatt acgaaaaatt
    26281 ttaaaaaaag tttgcaaaaa tcttgcattc tttttttgac tatgctatac ttatatacgg
    26341 tttaaaaaaa ctgcctaaga cagtagggga gctcgactca taaatatcct accgaggaca
    26401 aaacgtatca tgtaaaaaga agcgtattgt actttcgtgt ctaggtttgg gcgcgttttt
    26461 ctttttgaaa aattccccaa gcaaaataat tacggaggtg aacacactaa tgagtgaagc
    26521 aattattgct aaaaaagcgg aactagttga cgtagtagct gaaaaaatga aagctgctgc
    26581 atctatcgtc gttgtagacg ctcgtggttt gacagttgag caagatacag ttcttcgtcg
    26641 tgagcttcgt ggaagcgaag ttgagtataa agttattaaa aactcaatct tgcgtcgtgc
    26701 agctgaaaaa gctggtcttg aagatcttgc atctgtattt gttggaccat ctgcagtagc
    26761 attttctaat gaagatgtta tcgcaccagc gaaaatcttg aacgactttt ctaaaaacgc
    26821 tgaagcactt gaaattaaag gtggtgcaat cgaaggcgct gtcgcatcta aagaagagat
    26881 tcttgcactt gcaactcttc caaaccgcga aggacttctt tctatgctcc tttctgtact
    26941 tcaagcgcca gtgcgcaacg ttgctcttgc agtcaaagcg gttgcagaaa gcaaagaaga
    27001 cgcggcttaa tcttaagcta cacagcgtag cctagctacg aaaaaaacta ttataaaatt
    27061 taaaacttat ttggaggaaa taacaatggc attgaacatt gaaaacatta ttgctgaaat
    27121 taaagaagct tcaatccttg aattgaacga ccttgtaaaa gctatcgaag aagaatttgg
    27181 tgtaactgca gctgctcctg tagctgttgc tgcagctgat gcagctgatg ctggtgctgc
    27241 taaagattca ttcgacgttg aattgacatc tgcaggcgac aaaaaagttg gcgttatcaa
    27301 agttgtacgt gaaatcactg gtcttggtct taaagaggct aaagaacttg ttgacggtgc
    27361 accagcactt gttaaagaag gcgttgcaac tgcagaagct gaagaaatca aagctaaatt
    27421 ggaagaagct ggagcttcag ttactcttaa ataatagagc aactacatta gtagcttaaa
    27481 aacatgatta aaccgctatt cttaggagta gcggtttttc tttttgttta aaaaggggca
    27541 aactttttat atagttgttt aggaaataaa taatttttgt ataattggtt tactactcta
    27601 aattaacgta gtacaagagt ttgttaatta aactatacct tatggttttg actagtgtga
    27661 tataattatg ttgaaatact tatctggaaa ggtttttata atgagtttaa gaaataaaat
    27721 tgagcaacat attaaagagc tagagggagg gaagtttcaa aaactaggtg acgcgtacct
    27781 aagtagaaaa tacaatttta atattgtttc attaggctca caggaaggaa cagataaaac
    27841 aactaaaggg atcccagatt cttacgctgt cgaaaatggt aaatatgttt atattatgta
    27901 tggcacccat aagtctgtaa tatctaaatt agaaggagac attcagtcag ttaagaaaaa
    27961 aattttagag gaaaatattg ctgaagataa agttgggaga cttatttgtt gtcatacttc
    28021 ttcaaatata acaattaaac aaaaagaaga tctagaaaaa atggctgagc cttatcatct
    28081 agaattgatt ggaattaatg aaattgctaa tgatcttaca aagatagatt ttcaatattt
    28141 agcaaaagag tatctatcaa tcagtgaatc tacagagcaa gtttggtcta tcaatgactt
    28201 cataagaatt catgatgaga gtaaaactaa tgctcctata tcaaatgact atattggaga
    28261 tgtaagtgaa attataaata ccattaaatc aagtgaaaaa cgaatcttct taatatctgc
    28321 taagcctgga acagggaaaa ctagattagc aattgaaatt tgctctttgc tggatcgaaa
    28381 taaatataac attatttgtg tcaagagcaa taatcaggat atttaccaag atgtaaaacg
    28441 caatctaaat ttacacaaa
//
LOCUS       DAVKMU010000024        25787 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKMU010000024 DAVKMU010000000
VERSION     DAVKMU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.024"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            160..324
                     /locus_tag="VW343_001211"
     CDS             160..324
                     /locus_tag="VW343_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9755762.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            408..974
                     /gene="amaP"
                     /locus_tag="VW343_001212"
     CDS             408..974
                     /gene="amaP"
                     /locus_tag="VW343_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9755763.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            986..1156
                     /locus_tag="VW343_001213"
     CDS             986..1156
                     /locus_tag="VW343_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9755764.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1195..1803
                     /locus_tag="VW343_001214"
     CDS             1195..1803
                     /locus_tag="VW343_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9755765.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1834..2037
                     /locus_tag="VW343_001215"
     CDS             1834..2037
                     /locus_tag="VW343_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9755766.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2132..2410)
                     /locus_tag="VW343_001216"
                     /pseudo
     CDS             complement(2132..2410)
                     /locus_tag="VW343_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2608..2895
                     /locus_tag="VW343_001217"
     CDS             2608..2895
                     /locus_tag="VW343_001217"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9755767.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3173..3943)
                     /locus_tag="VW343_001218"
     CDS             complement(3173..3943)
                     /locus_tag="VW343_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9755768.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3958..4806)
                     /locus_tag="VW343_001219"
     CDS             complement(3958..4806)
                     /locus_tag="VW343_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9755769.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4803..6278)
                     /locus_tag="VW343_001220"
     CDS             complement(4803..6278)
                     /locus_tag="VW343_001220"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9755770.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6601..7956)
                     /locus_tag="VW343_001221"
     CDS             complement(6601..7956)
                     /locus_tag="VW343_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9755771.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7966..8283)
                     /locus_tag="VW343_001222"
     CDS             complement(7966..8283)
                     /locus_tag="VW343_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9755772.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8300..8617)
                     /locus_tag="VW343_001223"
     CDS             complement(8300..8617)
                     /locus_tag="VW343_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9755773.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8630..10315)
                     /locus_tag="VW343_001224"
     CDS             complement(8630..10315)
                     /locus_tag="VW343_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEU9755774.1"
                     /translation="MKNKKLILSLFILFLLIFIHAKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(10320..11216)
                     /locus_tag="VW343_001225"
     CDS             complement(10320..11216)
                     /locus_tag="VW343_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9755775.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(11219..12481)
                     /locus_tag="VW343_001226"
     CDS             complement(11219..12481)
                     /locus_tag="VW343_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9755776.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            12761..13939
                     /locus_tag="VW343_001227"
     CDS             12761..13939
                     /locus_tag="VW343_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9755777.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(14167..14613)
                     /locus_tag="VW343_001228"
     CDS             complement(14167..14613)
                     /locus_tag="VW343_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755778.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVQKYSRLKLE"
     gene            14683..15342
                     /locus_tag="VW343_001229"
     CDS             14683..15342
                     /locus_tag="VW343_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9755779.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACGIIFGKLLL
                     V"
     gene            complement(15394..16545)
                     /locus_tag="VW343_001230"
     CDS             complement(15394..16545)
                     /locus_tag="VW343_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9755780.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(16560..17291)
                     /locus_tag="VW343_001231"
     CDS             complement(16560..17291)
                     /locus_tag="VW343_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755781.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            17520..17621
                     /locus_tag="VW343_001232"
                     /pseudo
     CDS             17520..17621
                     /locus_tag="VW343_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(17593..18369)
                     /locus_tag="VW343_001233"
     CDS             complement(17593..18369)
                     /locus_tag="VW343_001233"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9755782.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(18362..19585)
                     /gene="trpB"
                     /locus_tag="VW343_001234"
     CDS             complement(18362..19585)
                     /gene="trpB"
                     /locus_tag="VW343_001234"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9755783.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGCPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(19563..20162)
                     /locus_tag="VW343_001235"
     CDS             complement(19563..20162)
                     /locus_tag="VW343_001235"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9755784.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     YFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(20149..20916)
                     /gene="trpC"
                     /locus_tag="VW343_001236"
     CDS             complement(20149..20916)
                     /gene="trpC"
                     /locus_tag="VW343_001236"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9755785.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(20913..21917)
                     /gene="trpD"
                     /locus_tag="VW343_001237"
     CDS             complement(20913..21917)
                     /gene="trpD"
                     /locus_tag="VW343_001237"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9755786.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIITGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(21928..22494)
                     /locus_tag="VW343_001238"
     CDS             complement(21928..22494)
                     /locus_tag="VW343_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9755787.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQTIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            complement(22491..23852)
                     /gene="trpE"
                     /locus_tag="VW343_001239"
     CDS             complement(22491..23852)
                     /gene="trpE"
                     /locus_tag="VW343_001239"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9755788.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEELEKALNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(24773..25774)
                     /locus_tag="VW343_001240"
     CDS             complement(24773..25774)
                     /locus_tag="VW343_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9755789.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         8103 a         5495 c         4131 g         8058 t
ORIGIN      
        1 taattgactt ttctatatta aagttatata atagtattaa tcaaagaaat tgattttttg
       61 atattaaaat aaaaaaggag ggtaggcagt gttgtgatca attattgctg gaggtcttat
      121 tggtctcttg gcaggtaaaa tcactaaaaa agtagttcta tgggaatcat cgcaaatgta
      181 ttcgctggtt tagtcggggc atatgcagga caatctcttt taggtagttg gggtccatca
      241 atcgctggaa tggctttgct cccatctatt gtaggtgcag cgattgtgat tactgtagtg
      301 tcattcttta caggtagaaa gtaaactttt cgccagtaaa gttagcaaac tatttttaaa
      361 tcaatgacgg gaaaaatagt ttaaatgtta aatcgaaagg attgtatatg tcaaaagcaa
      421 agaaaatatg tttcattatt ttctgtattt taatcttgac aattttcctt cctgttttga
      481 tagattatca tcaagttagt gatctaggta ttcatctact tagctggaga cagaactccg
      541 tagttgaatt ctatcttgct agatatgtct tttgggggac agtggttcta tcaactttag
      601 ttttattatc cattttagtt gtgatgtttt atcctaaacg ttacttggaa atccaacttg
      661 aaactaaaaa cgatacatta aaattaaaga attcggcaat cgaaggtttt gttagaagtt
      721 tggtgagtga tcatagattg atcaagaacc caactgttca tgtaaattta cgaaaaaata
      781 aatgtttcgt tcatgtagaa ggtaaaattc ttccttcaga caacatcgct gacagatgcc
      841 aaataattca aaatgaaata actaatggat tgaagcagtt ttttggtatt gagcgtcaag
      901 taaaacttga agttgcagta aaaaattacc aaccaaaacc tcaaaacaaa aagactgtta
      961 gtcgtgtgaa gtaaggaagt aaaaaatgga atggcttaaa caatatcgat atccaattat
     1021 cgctggtctc ataggcgtat ttctggcttg tttgattgtc tcctttggct tcttcaaaac
     1081 aatatttgta ttgattttag gagcactggg agttgcagct ggattatata tcgaaaaaaa
     1141 ctatatagat aaataaaaaa ataaaaatta ctaatttaat taaaggagtt tcatatgtca
     1201 aacgaaaaaa acacaaacac taacgtagaa aagaaagatg ctactgttgt agctcacgaa
     1261 atcaaagggg aacttactta cgaagataaa gttatccaaa aaatcattgg tctttcacta
     1321 gaaaacgttt caggtctttt gggaatcgat ggtggtttct tctcaaatct taaagaaaaa
     1381 atcgttaaca gcgatgacgt aacaagtggt gttaacgtag aagttggtaa aacacaagtt
     1441 gcagttgact taaacgttat tgttgagtac caaaaaaatg ttccagcttt atattcagaa
     1501 atcagagaaa tcgtatcttc agaagttgct aaaatgactg acttggaaat tgttgaaatc
     1561 aacgtaaacg ttgtcgacat caaaactaaa gaacagcatg aagcagactc agtaagcctt
     1621 caagatcgcg tatctgacgt tgctgaatca acaggagaat tcacttcaga acaattcgaa
     1681 aaagctaaat ctggtcttgg atctggtttc tcaactgttc aagaaaaagt tagcgaaggt
     1741 gtagaagctg ttaaaggtgc agcaaatggt gtagtatctc acgaaaacac tcgtgtaaac
     1801 taagataaaa taaatataac aggagaaatt atcatgtcag tagaagaaaa attaaatcaa
     1861 gctaaaggtt ctattaaaga aggtgttggg aaagccatcg gtgatgaaaa aatggaaaaa
     1921 gaaggagcag ctgaaaaagt tgtttctaaa gtaaaagaag ttgccgaaga cgctaaagac
     1981 gctgtagaag gtgctgtaga aggtgttaaa aacatgttga gtggcgacga taaataaggt
     2041 taaaagttac tttatctttt tggtaacatt agtcaagatg attctcagaa aacaaaaagc
     2101 tagagattcc caattgcgga accctagctt tttaattttg cctctttctc ttattatatt
     2161 tcagcaggtt gttggccatg agtacgaatc ccatgtcaat tctcacttga cgcttccctc
     2221 tcagatgaca tctcttataa cccaaacaaa cctttatctg cccaaagaca gatttcatat
     2281 caatcttacg tttagcgaaa atttgtctac ccttggaaga taaaagtgcc tgatattctt
     2341 tagtttttaa acactggtaa cgttcattca tatacagtcc cttttgaggg gctgattcag
     2401 gttcataatc gcagtcaaca ttgatttcaa ggctgtttgc tttctatctc ccctttttca
     2461 tgatatattt tcgtacaggt gcttcaatag tttctatagt aaaatgaacc aaaaatagta
     2521 cacgttgcgg tataatgttc ttgtggcata ttcattagat tttcgtaaaa agttctctcc
     2581 tatttgtgag cgaacaggta tagtcaattg attgtcagtt tgaagaccaa gagtcgtaag
     2641 gcaaaggata tggcagaatc catccaaggg tggctggcac aatttctagt gaacctcttt
     2701 aaatctatta cgtttgactg tggaaaagag ttttcaaaat ggaaagatat ttccaatcat
     2761 cacgatagtg agagcttttt tgcgaatcta ggatgtccaa gacagcggtg tctcaacgaa
     2821 cattcaaata gacttttaag gtgtcatgac ttgccaaagc agacagattt caacgaagtc
     2881 agtcaagaat tttaggctgg gtaaaaactc aatttcagga gaaaatgaaa taaatattcc
     2941 cacaataaaa tgcatagtat caaagttttt ccaacacctg atattatgca ttttttaatt
     3001 ttaaagactt tttcccagcc tcaagaaatc acatcccacc taaatccttt ggataccaaa
     3061 tacctcatca ggtattttct gagttggtaa gactgtctag cttaatttga caaatggagg
     3121 gcgttaaatc cagtttttac aggaagtcta tttaaatatt ttcacactga ccttacaaaa
     3181 tatgaaacaa attatataaa gtcattccca tgacaaagaa taacatccct ttaaaacagt
     3241 atcttaccca attttcaggt aagacttttg atacaaccgt ccccaatacc cctccgcata
     3301 tagaagcaag aaaaataact ggaacaggtg ctaaatgata ctgatttaaa cctgtaacca
     3361 caacaattgt agccaaatga gacagctggg aaaagaaaat aattgcaaga gaatacatcg
     3421 ttgcttcttt tatcgaaata gaaaaaaaca ccatcaataa agatacattg agcggaccac
     3481 caccaatccc taaaaaacta gaaattaagc ccaatagaag accacaaatc atatagaatc
     3541 ctatcttatc aaattgataa gtttttttaa aatctgttaa aacaaatgat acaagtagca
     3601 tcacaaatag taaaatcatt tgaacaatgg atactagccg tactgataat tgagataaca
     3661 aaacttgaaa aataagatga cctagaaatc ctcctaaaac agaagaaaag gataccgtaa
     3721 aaatcaatct ccattttatc tctttacttt gtgaaaatct tttgattgtt gaaataatcg
     3781 ccattataaa aacagagaaa ctagaataaa aagcaatatc actgaccgaa tggtatccaa
     3841 aactatccat aagtggctta attataattc caccacccat gcctgatata gaacccaatc
     3901 cactagctat tataatgatt agaaaataaa taatctcaac cactttttaa gtccatccta
     3961 ttctttcaaa catctaaaac ctagatggct actagtggaa gtagaaatac atccatttct
     4021 agcagctact cggtatcgat tacaataaga acaatgacat aaaaaggaac ctcctctgat
     4081 agcatactcc tcatcttgta tcccatattt tggcaactca tagtcatctt cattaaaact
     4141 agctaataag gtatatctcg gattgcgaca ccattcccaa acattcccaa tcatttgata
     4201 tagaccaaaa tcattaggta aaaattcata gacaggagcc gttcctataa atccatctaa
     4261 ggcagtattt tcataaggaa atcttccttg ccaagtattg gcatgataat atccaccttc
     4321 taaaagagta tctccccaag gatattcaga ttgtcttcct cctctagcag catattccca
     4381 ctgtgcttca gtcggtaaag acattccgct ccaattacag aaagccaatg catcttctaa
     4441 agcaacatga actactggat ggtcttctaa acccactaga ttactattct caccgtaggg
     4501 atgtttccag caagcgttcg gaacttgtaa ccaccacgga gccccagcag gatgaggata
     4561 tccctccctt tctgcctctg gaacgaacaa tataaaaaca aaagaccact cttgtcgctc
     4621 agctagtgtt ttatagcctg tttctttcac aaattgagca aattcttgat tcgtaactgg
     4681 agtatccgca atggaaaaat cctttacact aacagcgact tgaggacctt caaaatcatc
     4741 taaaaagccc tcttcagaat ttgttcccat cataaatgtc ccacccttga tttttttcat
     4801 tttcatgatt gactatcccc cgttttcgtt agagttggcg ttattttaga aagttcaaca
     4861 ggaactaact cttcatccac aacaaaacct tcttctctat atctaagaaa gtctactaat
     4921 tttttcttca tctgacgaac aataggttga tatttttcac ttggatacaa atcattcatt
     4981 tcatgcggat cttttttcat atcaaataac tgataatgat ttaaaacagg aaaccagata
     5041 aatttccact ggtctgtcaa aatatattga ctagaatctt ttcctaaagc atgttcacca
     5101 tgaaattctg ttcgccaccc ctcgtactgt ccaaataata aattcttaac actccgtcca
     5161 tctagttcat ctgtagtagt tcctcctgcc aaatccacga gtgaaggaaa aatatcttga
     5221 attttcacta attgcttgat tgttccccta ttaccagcta tcaatccagc cggatcgtaa
     5281 ataaacgaag gaatgtggat acttccctga taaggatatc cctttctaaa cagataatgt
     5341 tcaccaagct gatctccatg atccgaaaca aaccagataa tcgtatcttt atcatgacga
     5401 aattctttta gggcagtcaa aaagcgacta atttgatggt ctatatgagt aatcaatcca
     5461 aaataagcag caaccattct tctttgatca tcttccttta atttccctct taaagcataa
     5521 atagacggaa tttgcttttc taatacttcc caattcccga tatgcaaatc gagaaattga
     5581 ggcaagcgtt ccatatacat atcaaaatag tattttgggg gatttaatgg agcatgtggt
     5641 ttttcaaaag acattttgag aaaaaaagga accgttggat ctcttctttg taaaaatgaa
     5701 atggactcac tcacaaccca gtttgttgga tgtaacttct catctttatc ccaaggtcta
     5761 gcttcccaag aattacaatc cattccgtca tcaatcaaat caacatcata accaacttta
     5821 ccctttaaaa aggctagata gtcactagca taatcaaatt gacttccata agccttatca
     5881 tactttctat caacatgtag atacccatcg tgaagtaaga catgatcaaa cccaagtctc
     5941 tgtctggaag gaaaaacatg catcttacca atacactctg tttgataacc catatcttta
     6001 aagacttttg gaagagtatt agtaaaattc caaggcacct catcttgata accaactcgt
     6061 ccacttttat cttggtctaa gccagtcaat aaagctgccc tagcaggaac gcaagaagga
     6121 actggagaat aggcattttc aaaattataa cctacactag ccatcatatc taatgttgga
     6181 gtgctaacta gcttatcctt actattcaag gataaggcgt ctgctctcat ttgatctaca
     6241 acaatcaaaa taatatttgg ctgttttgtc tgaaccataa aatctccttt ctaatatggc
     6301 aaaagaggca caagaagata tctaccttta ctgcacccct ttctatatca atctctctat
     6361 ataaaacaat aacattcttg ttatgtttta tagaaaaatg aactaaaata tgactaaatc
     6421 gattaggaaa ttcaaatcat tttctagtac tgttttagaa gttacagtgt actattccaa
     6481 cttcaataaa ctataaacct ttgtctaata acaattttag tggagataag aaatcctaca
     6541 cctaactcat cttacacgta atctatttct attttatcac aaaaaacgca agtaagacca
     6601 ttaactcaat tcagttttat ctgccatttt cacaaatggg aaataagtca agacactaat
     6661 aatcaaacaa acaactgata agatgatggc acgccaatca aatgctgtag agaagaaacc
     6721 atataaaatt ggaggcatta cccaagtaac gttttgtgta acaggtgaaa caagacccca
     6781 gcttgttgcc cagtaagcta ccgttgccat gaaaaccggg ctaagtacaa atggtataaa
     6841 tagcaaagga ttcaagacaa ctggtaaacc ataattgacc ggctcaccaa tattaaacag
     6901 aactggtgct agaccaagtt tagcaacttt tcgataatga ctgtttcttg aaaaaattaa
     6961 aatagcaagt actaatccta atcctccaaa ccagacaaac gccccaaaag acccacttgt
     7021 ccatatataa ggaatcggtt cacctttttg gaaagcatcc agattcgcta acatagcaac
     7081 tccaaacagc ccttccatga tgggagccaa tacatttcct ccatggagac caaaaaacca
     7141 gaataactta ttcaaaaaga tcatcagaat aactgcaaag aaactttgag acaaacctag
     7201 taatggcgtt tgtaacacct tgtaaaccca atcaatcaat aagtcattgc taagtaaatg
     7261 gaaaacataa gtcaagatgg ctactatata catcgccata aatcctggaa tgatagaagt
     7321 gaacggctta gcaatcgcag ggggaactga atctggtaac ttgattaccc agttcttttt
     7381 cattacttta cagaaaataa tagaggctaa aaatccaatc atcatggctg taaagtagcc
     7441 tctggcatta atatggtttc ctggaatcac attcccaata gttaccatca gatttttacc
     7501 atcaaatgct agattatcaa ttccttgtaa gatttgatct aatttcacat ctcctacatt
     7561 tgccaaaggg aaactctttg taactgtact tccaatcgaa atgacaaacg aagcaagtga
     7621 taccaaacca gcagaaactg tatcaacctt gtaaatctta gcgatattca ctcccaagca
     7681 atagatgaac aacaaggaaa caattggtat acttcccttg aataccaaat tattgatgtc
     7741 aacaagccac tgaaaggttt tcgtaatact ttctaggtga aattgttgtg gtaaatccac
     7801 tagaaaagca tttaataaca aagcaatgga acctgtcata ataacaggca tagtccccac
     7861 aaatgaatca cgtaaagcaa ctaaaaactt ctgattccca actttcattg caattggaga
     7921 aagtttttct tgcattttat ccaacattgt attcatacca aactcctaat ttttttcaaa
     7981 tcgttcgtat acttctacca cttctttcgc caagtcaaca aaagtcagac tcgtcattaa
     8041 atgatcttgc gcgtgtacca tcaagaggct aattgatgtt ttttccccct gtgcctctct
     8101 tgtcaacatt tctgtttgag ccttatgcgc ctgtaataag gcagcattcg catcagctaa
     8161 tcttcgattg gctttagaga aatccccttt ttttgctgcc tgaatagcct ccatagcatt
     8221 acttttggct tccccaccat acataattag ccccattaca gattctaaat tactttcatc
     8281 cataacattc tcctcaatct tagtctaata aactttcagc taaatctaaa actttagaac
     8341 cattcatcat tccgtaatct gtcatcggaa taaccgccac aggaatctgt ctatctttta
     8401 gtttttcttg aaagtcccct agtaaatagc gaacttgagg acctaaaagc aacacattta
     8461 cttcttttgt tgctacaatt tcttctgctt caggagctgg aactgcaaaa atttctgcat
     8521 ccaacccctt atcttctgct gccttttgca tctttgtcac tagcagactt gtactcatac
     8581 ctgctgcaca tgctaacata attgttactt tagccatagc ttactccttt tattttattg
     8641 tcaatgttac tgtttttcga tcaactttag actcataatc taaatgtttg ggacgactag
     8701 tataatgcaa attataaact cctggaggca tttgtgaaat cttttctttt tcagttttat
     8761 tgcgaattaa taaatctacc gaagtattcc ctccaaagtt attaaacata ggttcatttt
     8821 caatagaata atctaactca tatccactat tagttgtaat tccttttaat aaatcaggaa
     8881 catctcctct gtttattact atatccttag cgccatttat tttgggagat tgactataat
     8941 actcttcagc ttgatgttgt tgtaaatcat ttctagagta agaaacataa tcaactacaa
     9001 attcagtccc tgataaatta ttgtccacta ccccaggcca acttcctccc atcgctaaat
     9061 ttaactgaac atactgaggt ctattaaaaa tctttttcgc tatattatct gatccataat
     9121 atacttttct aacaatatga ttgtcaacca tccatacaat ataatcttta taccaatcaa
     9181 ttgaaaaagt atgatacgaa tcattaaagt ttccactcga taatttaaag gctgtaccac
     9241 gtcctgcaaa ttttccatca tcattatccg actgtccata atgaagcgtt tgataaactg
     9301 tcttattccc acttccattt gaatttcgtt caccaactaa ttccattata tctatctcac
     9361 ctgttgacgg ccatcctcgt ccctgaacag gattaatctt cccatcaagt gtaaaatccg
     9421 atccaagagt ccagaaagca ggaaaaactc cttgaccttt cggtaatttt gcacgcatct
     9481 ctaatttacc ataaagaaat tctactttcc catgtgttct aacacttcct gaattatata
     9541 caacctttct gtgattgttt cttgggttaa aatactgatc ttccttagag cgattcgtag
     9601 ctctaagaac taaatttccc gaacgtagaa aaacattctc atcacttctt acataatgtt
     9661 gctgttccca accacgaatt gatcctaatt catatcccca atatttttta tttaattgtt
     9721 cgccatcaaa atcatctctc caaattttct tatatgaatt tcctttagac agttgataca
     9781 tctctacatt atcaataaaa atattagatt tttttgtagt atcgtcttct gaccatttca
     9841 ctaattgaat caaaacttta tcgtgtttac cggaattgaa agaaaacatg tattttttcc
     9901 attcggttgt attaactgtt tgatcaacag cttctccaat gttattttga acaatctctc
     9961 cttgcttatt attatcaaac catcttgcgg taaaatatcc ctctgctcca ctaatatcta
    10021 ctttggcaaa agcagatact aaataatctt cattaggttc tatatcaata acttgtccaa
    10081 tatatccatc tgctctatta tcattaattg cagtagaagg aatcatacct gagtaatgtc
    10141 cgttactata actctcattt gaattaatta ttcccctatt tctagagttc caaaacagct
    10201 ctccttgctc aaaatttgaa ttttttatta aattttcatt agcaaaaact ttcgcatgaa
    10261 taaatattaa taaaaataat ataaataaac ttaaaattaa tttcttattc ttcattttat
    10321 cacctattta ctgacctagc caagcgagat actgtatgtc tcactcctct aattgccttg
    10381 ctcaatgcat aaatattatc gactgttgcc agtcccccat atcctgcgtc acctatatgt
    10441 tgaatatcaa ctccacaaat tttatttcta agtgcaattt ccttgatagt atctgtatca
    10501 gatgtttctt ggctagtacc aatagcactc aatacaagac cacccttact atgaacgaga
    10561 tcaacgactt cacgcaactc ttggtcatga aaggctggaa cggttccaac tgctggaaca
    10621 agtatcacat ccgcacctgc ttccaacaat tgctctgcga cagaaagctc tgccacaggc
    10681 tcattcactc ctgccccgtg cattttacca gctataatca aacctgagaa attctcttta
    10741 gcagattgaa ctgctttgat aatctcttga ttgctaactc ctgttccagg atttccagtc
    10801 aaacagacaa agtcaaaacc taactcctct attcgattca atgtttcaac actagcaaca
    10861 cgacctgcaa caatttcctg tgtttcctct agcatctttg cagatgggtc aatcggttcc
    10921 aaatttacac caattggaca agcaacaagc tggtgtaagc gatgaataac atcatcacct
    10981 gaactatcca aagcataaat tttaggctca aaaacatcaa cacaattcaa taaaatcata
    11041 tcagctccaa aagctctagc aatttcagaa ttagttatat ccccaacaaa tgtctcacga
    11101 gtaactacat tttcagatag aacaactcta ccttcacttg ctaaaatact ttgttttaat
    11161 tcttcagcat tcatctgtaa tatctcagat ggatttgcac taattaaacg tttcattctc
    11221 aatcctccaa taacttaaac cgtttttcaa tagctattac aaatggtaaa taaagtaaga
    11281 catccaaaac aatcaataca atttgtaata atgaaccaga aatacttcct gtagcaagta
    11341 aaccacttat aacaggtggc atagtccaac ctggatctgt ataagttagc ggaaccaaac
    11401 ctgatgccat tgctccccac gctactagta agttaaacat tggagctaag ataaatggaa
    11461 ctagtaacaa aatgttcaaa acaataggaa cgccaaacat tgctggttca tttatattaa
    11521 ataatccagg tataacagtt ataggtgcca aagtttttag ttgtttacta gctttcttct
    11581 tatgtgctaa atatcctaac gcaagaacta aacctatggt tgcaccaccg ccacctatga
    11641 atacaaaatt atccataaat acgctagtaa aaatatttgg aagagtttct ccagtttgat
    11701 aagctacttt atttgcatct agattagcta accataacgg tttcataact gcattaacaa
    11761 catttcctcc gtgtaatcct acaaaccaaa aactactatt aaggaaaatt aagataagaa
    11821 gaccaataac attatttcct aaaagtccaa gtggacctcc caaagctact tgagcaattt
    11881 catgaagatt aggtaaactt aatttatcta aagttgcata aacaatcaac caaccaacaa
    11941 tagtcactgc accaggaata atggcactaa agcttttaga tactgctggt ggaacaccat
    12001 ccggcatttt tatctgtata ttatgattta taaaccattg gtagatatag ccattaatca
    12061 atcctaaaat catagcaaca aataaacctt ttgatgccat gaaagcggta ggcattcctg
    12121 ctccagcctc tcctctaatg aaaggcgtaa ctgtaataaa agatgataat gacacaatac
    12181 cagcaggaat accatcaact ttgtattgtc tagccataaa gtaagcgata ccaaaactag
    12241 ctacaagacc aagtaatcca aaactactat caacaccctt ccataaatag tcagcaacac
    12301 caatatcacc cagatatttt tcccatcctg gaataggaaa acttgcaata accattagta
    12361 atgaacctat caacaataaa ggtatagtta gagtaatacc gtctcgaata gaaaccaacg
    12421 ctttattatt acccaatcta gccgctactg gaagtaactt ttcttgtaat aatttttcca
    12481 taaccttttc ctcttaaata tattttatta tcaaaagtta tcaattatct ttattttttg
    12541 acgtcaagtt gttatttgat tatcttttgt tatttatatt ttacaactat aagaaagcct
    12601 tgtcaaggat ttgtgtaaac atttgattat tttttattat caatatataa taaaaagaga
    12661 ccagtaagta atgaaaagct taaaaaaatt aacatctaca tttatccata gcaaaaaatc
    12721 ataggatttt aagaatctga caactaggaa gaaaactaat atgaccttat ctaagaaaca
    12781 attacaacta agagctaaaa tactggaaac agtttacaca ttaggtccca tttcacgaat
    12841 tgaaatcgct actaaaacag gaataacacc tgcaacaact agcagtatca caaatgatct
    12901 gattaaagaa aatattctct tagaacttgg tgaagacgaa cacgatacaa gcgtagggag
    12961 aaaaaaaatc ctgctagaca ttcaagctaa acgattttac tacattggtt gtgaactatc
    13021 tgaaaaacat ttcacttttg ctttaggaga taatctagga aatatcttaa aggaagagaa
    13081 agaaattgtt actaaacagt taatccaaga aaagggaaat caactcatta accagacact
    13141 aaagcaattc ttaaataact gctctgatta tgaaatagaa gcaattggaa tcgcgctacc
    13201 tggtcgctat ttagataatt ataaaataac aacaaacaat cctttatggc aacatatcga
    13261 cttagaaatg attcaatccc actttgataa gcctttgttt ttctctaaca atgttaactg
    13321 tatggctata ggtaaacgcc tatttagtcg ccaacagaat gaccctaatt ttgcttattt
    13381 tcattttgct agaggtatgc actgttctta tatctacgac ggaaacattt atgggaaagg
    13441 aaatctaatg attggagaaa tagggcatac agttgtttca tctgagggag aagaatgtag
    13501 ctgcgggcgc aagggatgct tacaaacttt tgctggagaa tcttggttaa tcaaaaaatc
    13561 aaaaattcta tatcatcaat ctccctattc tctacttcct agtttagtca aaaatgccga
    13621 tgatattgat atccaagtta ttttaactgc ttatcaatta ggtgacactg gaataatcac
    13681 acttatccat caagccttac tctatcttag tcaaaccatt ttaaatatca gtatgatgat
    13741 tgactctcaa aaaatctacc tacatagtcc tttactaaca aatcaacaca tcatccaaaa
    13801 actatatagt gaaatgaact acaaacccaa actgctctat aatcgcctac ctgaagtcat
    13861 tattgaacct tacaatgatt ttacagccgc acactcagct attgcacttt gtttgtatca
    13921 tacaattcta cattcttaat tttctattac atttaataaa taggctaaaa agagggagta
    13981 tatcataaaa agcgaataaa cctgttttct gaaaatcaga attctgtttt gttcgctttc
    14041 tttatatttg gaattatact cctatctccc ctttttcata atgtatagta aaatgaaata
    14101 ataacaggac gaatcgatcg ggacagtcaa atcgatttct aacaatgttt tagaagtaga
    14161 ggtgtactat tctagtttca atctactata tttttgtaca ggtgcttcaa ccatttgaac
    14221 gatttcaaat ccttcttttt ggtaaagatt ctgagctctt tgatttgcct cgaagacatt
    14281 tagagaaata ctatctatat cttcattttc aaaggccaaa ctaacaaatt tccttaaagc
    14341 ctggctacct aagtcttgct cctgtttctg ggggttgata aaaaatctcc cgatatgaag
    14401 attgctgtct tctagcctga ttttctggat aagtcccaca aactcttgtt catcaaagat
    14461 tgaaaagact ccttccaagg cttgaagggt cagtagaaaa ggaatccttg gtcccatcca
    14521 ttgttcttga aaggatttgc ctagggagtt ggaccactgg catacaaatt gagcgttttc
    14581 tatactcacc ttttcttcaa aacgaattgt catcttttcc tcaccatctt atctatgttt
    14641 ctccattata ctatttctcc cattttttac gaatagataa gtatgattga tttttatttt
    14701 tttctcgtcg ggagcattct cgcttccttt cttggtttgg tcattgaccg ttttccagag
    14761 caatccatta tcagttcagc cagtcactgc gattcctgtc agactcgctt gcgtccctta
    14821 gatttgattc cgattctctc acaggtcttc aatcgctttc gctgtcgcta ctgcaaagtt
    14881 cgctatcctg tctggtatgc cctctttgaa ttagtcttag gactcctctt tctgctttac
    14941 tcttgggaat tgctttcctt ggggcaagtc gtcctaatca ccgctggttt gaccttgggt
    15001 atctacgact ttcaccatca ggaatatccc ttactggtct ggatgacttt ccacctaatc
    15061 ctaatagctt cctctggctg gaatctggtc atggtctcct tccttgctct tggaattttg
    15121 gctcatttta tcgatatccg catgggcgca ggggatttcc tctttttagc ttcttgtgct
    15181 ctcgtcttta gcgtaacgga gttactgatc ttgattcagt ttgcttctgc gacgggtatc
    15241 ctggcctttc tcctgcaaaa gaaaaaggag agacttcctt tcgtgccttt cctcttactt
    15301 gctgcttgtg ggattatttt tggtaagcta ctgcttgtct gataaaatcc aatttctgcc
    15361 atatgtcctt catgaaatta tttcacagtt aaattataaa ttatttcttt tgtacaaagg
    15421 gatgatgtta tcaaatcgat ctgttcttct atcttcttga tactgatcaa aaaatttcat
    15481 ttcgactgaa aatatttcgc ttataaactg taaacgaata ctttgtttag acattatagt
    15541 cgctagactg actagatgat tactcaaaac gacgtccaga atactcttta ctttgcttgg
    15601 ttttttaaca aaaatttgat catccaaagg ttcaatcatt ttgtaacctt tttgcgcaat
    15661 ttgacgataa aagtaagaat gttgctttgg agtcaataat cctaacttaa aagctcgata
    15721 ctctaaagcc tgtatcgaaa cattcaaatc cgacttcaat aaaatataac tatcaggatt
    15781 gctgacacgc ttgccaaccc tctcttcaaa tttgactaaa aactcttctt ttggcaataa
    15841 aaaacatgat gcaaaataat ttgcttcttg ctccaaacga ttgccatctt cattcatatc
    15901 tttatattta tgtaaaagaa tatgtcctag ctcatgagct aagtcaaaat ttcgacgtac
    15961 agatgattta ttcgttccca acacaatata aggtcttccc aattttgacc atgcgctata
    16021 agcatcagct tggccattaa ttaatcgttc cacgatatag atacctgaac gttctaattt
    16081 ataaagcaaa tcatgattat cttttgaaat acctaatttt tccctggcat aaagagccaa
    16141 ttcctcaatg gattctccct tatgataaga ttcactcact acattactta ggtcatgaat
    16201 tataatatta ggtataatta caaaactttc aaaataatca atcaaactat ctaccttatg
    16261 taaatacata gtttgaatat ctattgtttt ccgtgttgct aggtctgcat ttctaaaggc
    16321 aattacagaa gaatcaaatc gaatgctctc ttcttcctgt tcaaaataag ttaaatcaac
    16381 atgaaattgg ttggccaaat gcattttggt tgataattta ggtttcgttt cgttggactc
    16441 aaactgccaa atggcttgtt ccgttaaatt aattctctga gctaattctg ctctacttaa
    16501 accatttaac agccgtaatt ctttcaatac ccgaccatta aacatttata tactccttac
    16561 tacttttgac cttcttgttt ttctattctt ggaataattt caaaatcttc tgtttccgat
    16621 aattctgaaa aattaggaat atcttgatat ttagcttctt cgaaatggta cgaacttgat
    16681 tgaatatact cagacaaatc ttgaatcaag tgtagctgac cagttcgtgc atcaggcata
    16741 acaacatcta cagatataat attgttttct gagtccgcct cataagttaa aatcataaat
    16801 ttttcgatat tcgaattttt agtagcttgt tcaatttctt gaatcatttc atcagaaact
    16861 aactccatct gaattggaaa ggaatgacta tcttcatcat ttttgtagga agaatgttga
    16921 ttaagataaa gtgtattcat ctgagcgtat tcaaataagt agccactctt atttttttgt
    16981 accaaaggaa attggttcgt aagtcgcttc ttacccttta taattaacaa tactttccca
    17041 tatttttctg tatttgtttc aaattctaaa tatccccaag tctgtcctgc taattgtaat
    17101 ttatactcaa acaaatctgc tgatgcaaat gcagtatcaa tatgattagg tcgcgtccat
    17161 gcataaccat tcgacactat cattgtctct cttttttcta gacgttcatc tacataatct
    17221 ttttgccctt tcatcaaagt atctacaatt ttttgtgcct caagcgaatc aaagagatcc
    17281 tgattcaaca taattcttcc tcctccaaat actttttaat gaattatatc attttcttaa
    17341 agaaattact acaataatta tctttttctt aaagttctgt gtcagagtaa tttagaaaat
    17401 tatatcttct atagtaaaat caattaaaaa ctgaacaaat ttattgggaa attcaaatcg
    17461 ctttctgaaa atattttagg aaccgtagtg taatattcca gattcaattc actataaaac
    17521 tgacctttct cctgcaaaag aaaaaggaaa gacttccttt cgtgcctttc ctcttacttg
    17581 ctacttgttt gattattttt ggtaagctac tgcttgtctg ataaaatcct gaatcggctc
    17641 tccttggtgg agagctttta ctatttttga accgacgata acaccatctg acaccgcatt
    17701 gaagcgttcc agatcggctt gactagatac accaaaacct gtcaagactg ggatgtcggc
    17761 cacttgatga agttgcgcca agtgcttgtc caaatctgca cggtaattgc ctgatttccc
    17821 tgtcacccca ttgatggcaa cggcatagac gaatccctcc gcgccttcaa tcaactcttt
    17881 ctggcgctca attcctgtgg tcaagcttac taaaggaatc aaggcgatat ctgtatctgc
    17941 caaaaatggt tctacaaagt tggcatgttc atgaggcaag tctgggataa tcaagccctt
    18001 cacagctgta tcagccagat ctttgacaaa gttctccaca ccgtactgaa agagggggtt
    18061 gaagtaggtc atgatgacca gtggaatctc tgtttcaatg gttttcaagg tttcaactaa
    18121 agcctgggta gaggtcccgt gggctaaact gcgcaagcca gcttcttcga taacaggtcc
    18181 atctgcaaca gggtctgaaa agggaatacc cacttcaatt gcagagacac ccaaatcttc
    18241 tagaaagtgg attgtttctc ctagtccatc caaacctttc tcgtggtctc cagccatgat
    18301 atagggaaca aaaattcctt ttccagctgc tttaatagca tttaattttt ttgttagtgt
    18361 cttaggcatg agcttctccc ttctttgctg catctgcttc caagcggtct ttgacttgaa
    18421 ccacatcctt gtccccacga cctgataggc agacaatcat agacttttct ggtccaagtt
    18481 ctttggccaa tttcaccgca aaggcgatag catggctaga ttccaaagct gggataatcc
    18541 cttccacacg agacaagagt tggaatcctt ccaaagcttc ttcgtctgtc acagggacat
    18601 agctggcacg tttaatatcg tggtagtgag aatgctctgg accgatacca ggatagtcca
    18661 aacctgctga gatagagaag gcttcaagaa tttgaccatg ggcatcttgg agcacatcca
    18721 tgagggaacc gtgaaggaca cctggacaac ccttggtcaa ggtagctgcg tggtgctccg
    18781 tatccacacc aagtccagcc gcttcagctc catacatggc tactgactca tcttctacaa
    18841 agggatggaa gagaccgatg gcattcgacc caccaccaac acaggctact agggcatctg
    18901 gcagattttg tcctgtcaag tcacggtact gttgtttagc ctctcgacca atgacacttt
    18961 ggaagtcacg aacaatttct ggaaatggat gaggccccaa ggcagaacca agaatatagt
    19021 gggtatcatc gatattagcc acccatgaac gaagggctgc attaaccgca tccttgagca
    19081 cgcgcgaacc atctgtcacg gcctcgacct tagctcccaa aagctccatt cggaagacat
    19141 tgagggcttg gcgtttgaca tcttcctcac ccatgtagat ggtacattcc atgttaaaga
    19201 gggccgcagc agttgcagtt gccacaccgt gctgaccagc acccgtttct gcgataattt
    19261 tctttttacc catgcgtttg gcaagccaaa cttgtcctaa ggcattgtta atcttgtggg
    19321 ctcctgtatg gttaaggtct tcccgtttga gataaatctt ggctccgccg atatgctggg
    19381 tcaagttttt tgcgtaataa agaggagttt cacgtcctac gtactggcgc aaaagctggt
    19441 ttaattcctc ttggaaactt gggtctgcct gactttcacg gtaggccttc tccaactcca
    19501 aaactgctgt catcaatgtt tctgggacaa aacgtccgcc gaattttccg taaaatccat
    19561 ctttatttgg ttcctgatat gccatgcttt accctctcta taaatcttct aatcttttca
    19621 tgatcttttt gtccatctgt ctccactccg ctcgatacat ctactgcata gggagtaaaa
    19681 tattgaattg cttttactac attatcttca ttaaggccac ctgcgataaa gaagggctgt
    19741 gctagtccag tcgtatccag ttgaccccaa tcaaagggct ggccacttcc tgccacaggg
    19801 gcatcaaaga gtagataatc tgcctgagaa ttagggacat gcccatttcc atctacctgc
    19861 acagcctgaa tactggcaca aggcaaattc tcaaataaat catctgccac ctgaccgtga
    19921 acttgaacca agtccaagcc aactttgtca atcgcttcca gcagttctac ccgacttggt
    19981 gaaacaaata ctccaacctt tttcacatct gcaggaataa gctttgccaa ctcagctgcc
    20041 tcttctaaag tcacctgtct tttactaggt gcaaagacaa aaccgatata gtcggctcct
    20101 gctgaaacgg ctgtttccac cgcttctttg gtcgatagtc cacaaatttt aacctttgtc
    20161 aatctgcaac tccttgattc tctgggccac attttctgcc tgcataagag ctgtccctac
    20221 caaaattccg ttaaagtatg gggctagtcg ttccgcatcc tgccctgtga aaatggcaga
    20281 ttcagaaatg taatagcgac cttcctcaaa gtaaggggct aaatctacac tggtctgcaa
    20341 gtcgacctca aaggtagtca agttgcggtt gttgaccccg ataatctcag caccaagtct
    20401 gtgggctacc tctagttcag ctagattgtg agtctccact aagacttcca gaccaagctc
    20461 tgtcgcgtag tcatacagtt ccttgaggcg ttcttcggac aaggctgcca caataagcaa
    20521 gataactgtc gcacctgcat tgcgagcgcg gatgatttgc ttttcatcta tgataaagtc
    20581 tttgttgagc gtcggaatct ctacctgact ggaaatttcc cgtagataat ccaaatgccc
    20641 tttaaagaaa acctcatctg tcaacaccga aatcatcact gctccgtttt cttcataagt
    20701 ctgggcctgt tgcacaatat ccacatcgag attgatatct cccaaactag ggctagcttt
    20761 cttgacctca gcgattacct gcaagcagtc ctgatgattc ttcaaaaatt ctgccaagcg
    20821 ataggtctgg cgcagaggct ggatttgctc cagcttcatc tgctccacct cacgcgcctt
    20881 ctgctctaag attcgtgcta aaaattcctg actcattttt ggtactcctg taacagtctg
    20941 agtttttcaa gggccttgcc tctagcaatc acttgacggg ccaaggcaac tccttccttg
    21001 atgctatcaa tcttaccatt agcatagaaa ccaagaccag cattcaagac tgtcgtttcc
    21061 aagaatggac ttgcttcgtt tttcagaacg ctaagcaaaa tttctgcatt ttcctgagca
    21121 ttccctccac gaatatcttc catagcatag ccttccattc ccaaatcctc tggagtaaag
    21181 cttgacaagc tgatttcgcc attttcaaga agtgcaatct tggttgttcc gttcaagcca
    21241 gcttcatcca acccttctgg tccagcaacc acgatggcac gtttgcgacc catatttttc
    21301 aaaacctgag ctgtactttc taggagttct ggacgactaa ttccaagaag ctgtgtttct
    21361 aaagccattg gatgaatcag tggaccagtc aagttcataa tcgttggaat tcccaattcc
    21421 aaacgagctg gcatgatgta tttcatagct gggtgcatat ttttagcgaa gagaaagacg
    21481 atgccagttt tatcaaagac cttacctagt tcagctggtt tgaggtcaag attgattccc
    21541 aaggcttcga ggacatctgc ggaaccagat ttagaagaaa tcgagcggtt accatgcttg
    21601 gccatgtgaa taccgccacc agccaagaca aaggctgcag ttgtggaaat attaaaactg
    21661 aaagacttgt ccccacctgt accacaattg tccatggcat catgaatctc agttggaata
    21721 tgctgggcat gtcctctcat gacttgggca atggctgtgc gttcttcagg tgtttccccc
    21781 ttcatcttaa gagctaagag gagagaagca atctgcgctt cagttacacg cccagttatg
    21841 atacgctcaa tgacatccgt catttccaca cctgataaat tttcaaattt tgctagtttt
    21901 tcaataatct ctttcatcct agtttcctca ctttacaacc tcctcgataa aattccgaat
    21961 agaagacaag ccgtctggcg ttccaatgct ctctggatgg tactggaagc cataaatcgg
    22021 taggttttta tgttgaatcc ccatgatggt ttggtcatca gtcgaacgag ctgtcacttc
    22081 aaagtcttct ggcatttcct caatcaaaat actgtgataa cgcatgaccg cacgtccatc
    22141 ctcaatacct tgatacaaaa cagatggcgc ttcaaagttg atattgctct gtttcccatg
    22201 catgactttt ggagccaaac ctagcttacc accaaagact tctgcaatgg cttggtggcc
    22261 caaacaaatc cctagaatcg gcttcttgcc tgcaaaatca cgaatcatgt cttccatctt
    22321 tccagcatca actggccaac caggaccagg agaaaagacc agaccatctg ctttttcagc
    22381 ttcttcatac agcttggaat catcatttct cagaacctga acttctgcaa aattcccaat
    22441 gtattgggcc aagttatagg taaaagaatc atagttgtca atcaataaaa tcatggtctt
    22501 agttctccaa ttctagtcat agattttgct ttgttaatgg tttcttggta ttcgttttgg
    22561 gcgatagagt cgtagacaat ccctgcccca gcctgcacat aggctctttg atttttgaga
    22621 atcatggttc ggatggcgat ggccaaatcc atatcacccg tcgcagacaa gtagccgatt
    22681 gccccagcgt atactccccg tttttccgtt tccagttcat agatacgtct catcgctcga
    22741 atctttggcg ctccagaaac ggttccagca ggaagcgttg ctttcaaggc atccatggca
    22801 gtgagttctg caagcaaacg tcccttgacc acactggtca aatgcatgac gtagcggaag
    22861 agctccacct ccatatactt agtaacttgg acactggccg tttcagagat gcggccaata
    22921 tcgttacgcc ccaagtctac caacattcga tgttctgctg tttccttctc atcagagagg
    22981 aggtcagtcg ccaaggcctt gtcttcttca tccgtagccc ctcttggtcg cgtccctgca
    23041 atcggattgg ttgtcacgat gccatttttg acagaaacca aactttctgg actagctccg
    23101 atgatttgat aatccccaaa atcatagaaa taaaggtaat tagaaggatt agttacgcgg
    23161 agatttctgt agaagtcaaa tggatttcca gtaacttctg ctgagaaacg ctggctgagt
    23221 acacattgga acatatcacc attacgaatc aagtcacgag ctgtttccac catttcctca
    23281 aacttctgag gagcgatatg cggtttgaag tctaacggag atagatccaa gtcttcaaac
    23341 tcatttggag caggaatgcg taattcctca agcacttggt tcaaggcttt ttccaattct
    23401 tcttgactac gctcactata gagagcatcc tcgatgacat gtatcttctc cttcttgtgg
    23461 tcaaagacca tatagctctc atagacaaag aaatgcatgt ctggcgtccc aattgtatcc
    23521 tcagggattt gaccaatttc ttcataaagc gaaatcatat cgtaacccac aaaaccaatg
    23581 gctccaccac caaaagggag ctctgagtgg tgctgactct tatgaatcac ttcataaagg
    23641 aaatccaagg gatcccgatc aatcacttga ccattttgat agagaactcc attttcaaac
    23701 ttaatctcaa aaactggatt ataggctagg atagaaaaac gagccgtttc cttgtctctc
    23761 ggaatactct ctaaaataac cttatgttgc ccctttaagc gcatataagc caagattggt
    23821 gataagacat ctccatgaat gattcgttcc attgtaattt ccctttcagt tctaattcta
    23881 gttcgtggcg actgtatgaa aaatccccac gcaaaataac ttgcgtgagg acgaaattcg
    23941 cggtgccacc tcaattatag gatttctcct atctctcatt cctgtctcag atacctcctg
    24001 taacaggctg tgcgataaag ggcactccct tgagaatgat gttttcttct ctcgtttcag
    24061 atgaacccaa ctttacagct ttctctgctt gttttcagca accacaagct ctctgtgaga
    24121 gaaaagactg taatttttcc atctattatt ttttagcttc tagtaatctg caatcgcagc
    24181 taggtccttg cctccacgac cagagacatt gatgaagaga tgttcatctc ggtacacctt
    24241 tatactcttc gaaaatctct tcaaaccgcg tcaacgtcgc cttatcgtag gtaacgacgg
    24301 atagaaaaaa gcccacacac agaatatact tccgtgtgag ggcgttggta acgcggtgcc
    24361 acctcaatta taaagggact atccctttac atctctgcct tgtttaacaa caagctgcac
    24421 tgtaaggtgt gcgcaccgaa ttttcattgt ttcaaattca ttttcaaaat cagcccactt
    24481 tcactacttc caaccaccta ttcacaatca ccacaggctc cctgaagatc aaaaatagtt
    24541 acttttctga tttgttgaac ttattttaat actttgtttt ttctttgtca agactttttt
    24601 acgatttttt tgatatcatt cgaatgtgac catatcttcc ttagatcgaa catgaacatg
    24661 tcccacttgt tagaaattgg atccaactca atagaaactg aatggaggct aaacagaact
    24721 tattttagaa cactccatct tttccactag gattttcaag aattaaacaa tactagaaac
    24781 tctgtctcct aacaaattta ggagaaactt caacagatgt gacactttcc cctttaataa
    24841 ttgctaaaac accttctatc atttctttag ccaatttaac ataattggga gcaattgtag
    24901 acaaagctgg agtataatac tgagaaatag gaatattatc aaatccaatg atagaaatat
    24961 catctggaat aagaattcct ttctcatagc acgcacgaat caagccctga accttttcat
    25021 ctcctgaaac aaaaataatg tccggataat tttgggtagt caagtgctgc attgcataag
    25081 aataaactga atcaattgta gataagccat aaatgacttt taaatccata aagtaatttt
    25141 tatcattcag aaaagaacgc acacctcttt cacgatcctt attaacatga gattctcctc
    25201 ccataagcaa ccacatattt ttaaattttt cttcagttac agctttcatc atatcataag
    25261 tagcttgaaa attattatta gatacataga ctactccaga cgtttgagat tcaccgaaaa
    25321 caagaaaagg catatggttc ttctttaaat actgaattct gatatcatct acactttcat
    25381 aaaaaacaat aacaccatct actaggctac ctgtgcttga tataattgaa ttactaattg
    25441 tatcctcctc tccaaagtac tcaactatag cattaacacc aaattcttta cacgtccgta
    25501 acactttatc taacagcgta tgaaaccaaa ttaaaggaaa agagtcgatt ttttttacag
    25561 aaatcaatat atttatagct tcttttttag ttaaattttt tgcatacgca tttggaatat
    25621 acgacaattc ctctataact ttttgaatcg cttgataagt ttcttcttta acatttactc
    25681 caccattaat aactcgtgaa actgtttttg gagaaaaacc tgataaacgt gcaatatcat
    25741 aaatagttac ctttttccca tttatatttt tcatttcagt cctccat
//
LOCUS       DAVKMU010000025        25700 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKMU010000025 DAVKMU010000000
VERSION     DAVKMU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.025"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(127..780)
                     /gene="phoU"
                     /locus_tag="VW343_001241"
     CDS             complement(127..780)
                     /gene="phoU"
                     /locus_tag="VW343_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9755790.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(792..1550)
                     /gene="pstB"
                     /locus_tag="VW343_001242"
     CDS             complement(792..1550)
                     /gene="pstB"
                     /locus_tag="VW343_001242"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9755791.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            complement(1563..2366)
                     /gene="pstB"
                     /locus_tag="VW343_001243"
     CDS             complement(1563..2366)
                     /gene="pstB"
                     /locus_tag="VW343_001243"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9755792.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(2377..3261)
                     /gene="pstA"
                     /locus_tag="VW343_001244"
     CDS             complement(2377..3261)
                     /gene="pstA"
                     /locus_tag="VW343_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9755793.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(3251..4168)
                     /gene="pstC"
                     /locus_tag="VW343_001245"
     CDS             complement(3251..4168)
                     /gene="pstC"
                     /locus_tag="VW343_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9755794.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(4168..5046)
                     /gene="pstS"
                     /locus_tag="VW343_001246"
     CDS             complement(4168..5046)
                     /gene="pstS"
                     /locus_tag="VW343_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9755795.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(5620..6924)
                     /locus_tag="VW343_001247"
     CDS             complement(5620..6924)
                     /locus_tag="VW343_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9755796.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(6925..7698)
                     /locus_tag="VW343_001248"
     CDS             complement(6925..7698)
                     /locus_tag="VW343_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9755797.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     TYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(7688..7966)
                     /locus_tag="VW343_001249"
     CDS             complement(7688..7966)
                     /locus_tag="VW343_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9755798.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(7966..8367)
                     /locus_tag="VW343_001250"
     CDS             complement(7966..8367)
                     /locus_tag="VW343_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9755799.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(8577..9194)
                     /locus_tag="VW343_001251"
     CDS             complement(8577..9194)
                     /locus_tag="VW343_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9755800.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(9184..9957)
                     /locus_tag="VW343_001252"
     CDS             complement(9184..9957)
                     /locus_tag="VW343_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9755801.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(9968..10705)
                     /locus_tag="VW343_001253"
     CDS             complement(9968..10705)
                     /locus_tag="VW343_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9755802.1"
                     /translation="MCLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(10710..11840)
                     /locus_tag="VW343_001254"
     CDS             complement(10710..11840)
                     /locus_tag="VW343_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9755803.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(11924..12313)
                     /locus_tag="VW343_001255"
     CDS             complement(11924..12313)
                     /locus_tag="VW343_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9755804.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(12329..12712)
                     /locus_tag="VW343_001256"
     CDS             complement(12329..12712)
                     /locus_tag="VW343_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9755805.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(12713..13501)
                     /locus_tag="VW343_001257"
     CDS             complement(12713..13501)
                     /locus_tag="VW343_001257"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9755806.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(13494..14429)
                     /locus_tag="VW343_001258"
     CDS             complement(13494..14429)
                     /locus_tag="VW343_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9755807.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(14559..14735)
                     /locus_tag="VW343_001259"
     CDS             complement(14559..14735)
                     /locus_tag="VW343_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9755808.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(14875..15582)
                     /locus_tag="VW343_001260"
     CDS             complement(14875..15582)
                     /locus_tag="VW343_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9755809.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            15733..16761
                     /gene="queA"
                     /locus_tag="VW343_001261"
     CDS             15733..16761
                     /gene="queA"
                     /locus_tag="VW343_001261"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9755810.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKIVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(17422..17811)
                     /locus_tag="VW343_001262"
     CDS             complement(17422..17811)
                     /locus_tag="VW343_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9755811.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(17915..18556)
                     /locus_tag="VW343_001263"
     CDS             complement(17915..18556)
                     /locus_tag="VW343_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002871528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9755812.1"
                     /translation="MNTSFAHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRFEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKIEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKTLKQELLKKEPSPKFLSKKFQSKQNISMAQLLIKVKK"
     gene            complement(19270..19821)
                     /locus_tag="VW343_001264"
     CDS             complement(19270..19821)
                     /locus_tag="VW343_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755813.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSTENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(20220..21044)
                     /gene="nadE"
                     /locus_tag="VW343_001265"
     CDS             complement(20220..21044)
                     /gene="nadE"
                     /locus_tag="VW343_001265"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9755814.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(21041..22501)
                     /locus_tag="VW343_001266"
     CDS             complement(21041..22501)
                     /locus_tag="VW343_001266"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9755815.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIYNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFPEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(22617..23105)
                     /locus_tag="VW343_001267"
     CDS             complement(22617..23105)
                     /locus_tag="VW343_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755816.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(23095..23304)
                     /locus_tag="VW343_001268"
     CDS             complement(23095..23304)
                     /locus_tag="VW343_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9755817.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(23689..24417)
                     /locus_tag="VW343_001269"
     CDS             complement(23689..24417)
                     /locus_tag="VW343_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9755818.1"
                     /translation="MKLNKLNFLKESIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYAWNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQ
                     YLVLITTIILLYFFTLKKSKKYSF"
     gene            complement(24433..25224)
                     /locus_tag="VW343_001270"
     CDS             complement(24433..25224)
                     /locus_tag="VW343_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755819.1"
                     /translation="MNFALNKGEIVGLVGRNGVGKSMLMKILAQNNQPTSGNIISSDN
                     IGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLESGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
BASE COUNT         7709 a         5768 c         4573 g         7650 t
ORIGIN      
        1 aattataacg atatccaagt tacttgtcaa gtgtttttta aatttttatc tcaaaaatat
       61 tttttcgttc aaaaaaagga gccatcagtt gatttcaagc tcctttttat acagaattaa
      121 actattttat agttcgacaa tcttacctgt ttcaaagtag acaacccatt cacagatatt
      181 tttagcatag tcaccgatac gctccaagta ggaaataact tggaaataat cacgacccgt
      241 aacaatggct tctggatttt tcttaatctc ttcagtcgca aggtcacgga tagtttcaaa
      301 atagtggtta atttgctcat ccatggaggc cacccggtat gcgtcgtcaa cagaaccatt
      361 aagataaaga tcaagtgctg cttcaacaac gcttttaact tcacgtccca tttttttaat
      421 ttcttcctct acagctggaa tgcgctcttc ccccttcata cggatggttg cctgagcaat
      481 ggctacagcg tgatccccca tacgctccac atctgataca gccttaagga cagtcaagac
      541 tgtacgcaaa tcttgagaga ctggttgttg gagtgcgatc atttcaaatg atttcttttc
      601 cagtttcact tcgtattcat ttacttctgc atcatcttcg atgacctctt ttgccaggtc
      661 acggtcatgc gtgacaaaag cacgtaccgt acgattgatt tgtgagagca cttcttgtcc
      721 catagcgtag aactggttat gtaatttctc taaatcttct tcaaattgag atcgtaacat
      781 ctttcatctc cttatccaaa ttttcctgta atatagtctt ccgtttcctt gtgttgggga
      841 ttaaggaaca tctgcttggt atcattaaat tcaatcaaat ctccatctag gaaaaatcct
      901 gtcttatcag agatacgtga agcttgctgc atggaacggg ttaccagaag catggtgtac
      961 ttgtctttta gaccatacaa ggtttcctca attttaccag ctgaaatcgg atccaaagcc
     1021 gaagttggct catccaagag gatgatttta ggactagttg ccaagacacg ggccacgcag
     1081 acacgctgct gttgaccacc tgacaatcca atagctgaat catatagacg atccttgacc
     1141 tcatcccaga tagaggcacc ttgcaaggct ttttctacgg cttcatccag aacctgctta
     1201 tccttaattc cattgatacg aagcccgtag acaacattct catagatagt catagggaaa
     1261 ggattaggtt gttggaaaac cattccgatt tccttacgta attcaaccgt atctgtacgc
     1321 ggactgtaga tgttgtgacc attgtacacc acggatccag ttgtggtcac ctctggattg
     1381 agatctccca tgcggttgag agacttgagg agggttgact tccctgatcc agatggacca
     1441 atcaaggctg taatttcctt aggttggaaa gatagggaaa cactattcaa agccttcttt
     1501 ttattataat aaacggacag gtctgatacc tgtaaaatcg catctgtcat acggtttcct
     1561 ttctaaccaa agtgaccaga tacatagtca ttggtggact gtagcttggc attttggaaa
     1621 atagttgcag tcttgtcata ctcaatcaaa tcacccaagt aaaagaagcc tgtatagtca
     1681 cttgcacgag cagcctgctg catattatgc gttacaatga tgatggtaaa gtttttcttg
     1741 agctcaaaca tggtctcttc tagttgcatg gtcgcaatcg gatccaaggc tgaggctggc
     1801 tcatccatta agaggatatc tggcttaaca gagatggcac gagcgataca gagacgttgt
     1861 tgctgaccac ctgataaggt caaggctgac ttgtggagat cgtctttaac ctgatcccaa
     1921 agggcagcct gacgaaggga ggtttctacg atttcatcta ggacttgctt atccttaact
     1981 ccagcacgtt catgcgcaaa ggtaatatta cggtaaattg acttagcaaa tggattgggg
     2041 cgttgaaaaa ccattccaat gtgtttacgc atttcataaa cgttgatttc tggacggttg
     2101 acatcaattc cacgatagag aatctgccca gttactttag caatatcaat ggtatcattc
     2161 atgcgattga gactgcgtaa gtaggtagat ttccccgatc ccgacggacc aatcaaagct
     2221 gtaattttat ttctttcaaa ttgcatatca atccccttaa tggattcatt tttaccatag
     2281 taaacatgga catccttagt agaaagggct actttttctt caggaaaggt aaggatatgc
     2341 ttctcatccc agttatatgt tgacatggct tctcctttag gcagcggtta atttcttgtg
     2401 tagatagctt ccgaacttac gagctccaaa gttaaaaatc aggataaaga tcaggagcac
     2461 agcggcagaa cctgctgata caatggttcc atctggaata gtgccttcac tattgacttt
     2521 ccagatatgg acagccaagg tttctgcttg acggaagata gagatggggc tagtcacact
     2581 gaggatattc cagttagacc agtcaagagc tggcgccgat tgccctgctg tatagatcag
     2641 agctgcagct tcgccaaaga tacgaccaga tgccaaaacg acacccgtta caatacctgg
     2701 aagcgcttct ggaataacaa catgaaccac tgtctcccag cgagaaatcc caagagccag
     2761 accagcctca cgttgggtat ggtgaacgtg tttcaaacta tcctctacat tacgcgtcat
     2821 ctgaggcaag ttaaagactg tcaaggccaa ggcacctgaa atgattgaaa atccatactc
     2881 aaactggact acaaagatca agtaaccaaa gagacccacc accactgatg gtaaagagga
     2941 caagatttca atacaagtcc gcacaaagtt ggtaacagga ccttttttag catattcagc
     3001 caagtaaatc ccagctccca tagaaagagg tacagaaata atcaaggtaa tgaccaatag
     3061 gaaaaaggaa ttgtaaagct gaatgccaat cccaccacct gcttgaaaag cagaagacct
     3121 tccagtcaag aaagaccaag agatatgggg caagccccga accaagatat agagaatcaa
     3181 ggaagccaag attgtcacaa tgatgctagc aatcgtatag aggacagctg ttgcaagttt
     3241 atctaatttc ttagcgcgca taatttttct ttcctctttc tttcgtaatc aatttaatca
     3301 cactgttaaa aactaaactc atcaagagca gtaccaaggc cagtgaccag agaacattat
     3361 tatttacagt tcccatgaca gtgttcccaa ttcccatagt taatatagaa gttaaagttg
     3421 cagctggtgt ggtcaaggaa gttgggataa cagctgagtt tccgacaacc atctggatag
     3481 ctagagcctc accaaaggca cgcgccatcc caaagaccac tgcagtgaaa ataccagaac
     3541 gggccgcctt caagatcaca cgccagatag tctgccagcg agtggctccc atagcgaaac
     3601 tggcttcacg ataataacga ggaaccgcac gcaagctatc cgttgtcata aaggttacgg
     3661 tcggcaaaat catgacaaag aggacggaaa tccctgacaa aatcccaaaa ccagtcccac
     3721 caaagacact gcgaacaaag ggaacgacga cttgcaagcc aataaatccg tacactactg
     3781 aaggaatccc aaccaggagt tcaatagctg gttgcaaaat cttcgcccct tttggtgata
     3841 cttcggtcat aaaaactgct gcaccaatag caaagggtgt tgcgataagg gctgagagaa
     3901 tggtaacgat aaaggaaccc aaaatcatag gaagggcacc aaattcttta ctagaaggat
     3961 tccaagttcc tcccaaaaga aagtcaaaga tattcacacc attgacaaag aaggtcgaca
     4021 agcctttttg cgctacgaaa accaaaatca tggccacaag gatgactatc aaagaaagac
     4081 aggcaaaggt caaacctttt cctaatttct ccagacgaga attctttgat ggaagcaaca
     4141 ttttcttagc taattcttct tgattcatta ttgtctccct tccaacactg tcacagttcc
     4201 ggcagcatct ttttcaacct tcatttcctt aatcggaata tacttcaatc ctttgacaat
     4261 cccttcttgg gtctcatccg agagaacaaa attgagaaat tctgcagcca actcattggg
     4321 ctgccccaat gtatacatat gctcataaga ccacaagggc caattattgc tacttatatt
     4381 ttctggactt aagtcatagc cattcaactt catgcttttg accgaatcat ctatataggt
     4441 aagagataaa taagagatag ctcctggact ttttgatacg attgatttta ccgctccatt
     4501 tgaatcctgc tcctgacttt gcatggcaga ctgaccttcc ataatgacag tatcaaaggt
     4561 agcacgagag ccagagccgg ctgcccgatt gataacagag atgggtaagt ccttaccacc
     4621 aacctctttc caattggtta cctcacctat gaagatttga cgaagttgct ctgtcgttag
     4681 gttatcaaca tcaacctcct tattgacaat cagagccaag ccagctaccg cgaccttgtg
     4741 gtcaacaaga gcagaagcat caattccgtc tttttcctca gcaaatacat ctgagtttcc
     4801 tatatcaact gccccagact gaacctggga caagcctgta ccagaacctc ccccttggac
     4861 attgaccgtt tttccaacat ggatcgtgcc aaattcatct gccgctactt caaccaaggg
     4921 ttgcaaggca gttgagccaa cagccgttat ggattctcca cgatcaatcc agctagcaca
     4981 gcctactaaa caagccgtca gccaaaaagc gataagagac agagcaagct tttttctttt
     5041 tttcactgtt tttctcctcg aaaataatta tgaatactgt gaatttttta agtagttctt
     5101 tatgagttga cgcatgaatt cttaccaaat ttctgcgcaa ttgattattt atataatata
     5161 ggctatatta ctctttccta acctcctttt ttcatatgtg gataaaatct cttgtctatc
     5221 ccttccccca ttgtcaccca ttatagtcat ttcgtgtctc tttttcccct ttttaatgca
     5281 agggaaatta ctcttcttag atgataatcc aaaagctaga aaggtatctc aaacctctct
     5341 actctcccag actagtttac aactaaaagg aaaagattct attttatgag aaatctagtt
     5401 tacaagcggt aagaacgcta ataactaaac ttcttgtact ctttgaaaat ctcttcaaag
     5461 cagtgttttg agctatctat ggctagcttc ctagtttgct ctttgatttt catcgagtag
     5521 taaaactaca tgtgatggca atcaagatat caagaatcat cctactaaaa aaatccatac
     5581 tttcactata acatagaata agatatttga ctagcatttt catttgaatc tgaggccttt
     5641 tggaaaataa tttttcaaaa catttccagt aacctttgca aagcccaagc cattgccttt
     5701 aaccaaaact tggtaccaac catttggcag actttctgcc agctgaacgg tttctccagc
     5761 cgcatacttg acaaacgctt cttggccaat ttcaaccgac tgttcgacct gactcggttt
     5821 caaggctaaa ccaagagcga aactgggctc aaagcgtttc ttcttaaaag tacccagatg
     5881 cagtccattg cgagcaatct tgagcttccc taaatctggc aaaagttctg gcaagagata
     5941 aagctggtct ccaaaaatct gcaagatacc cggtagattg accttcaaat ggttttgggc
     6001 aaattcctgc cacaaggcaa cttgttcacg gctgaggtta ctcttacttg ccttaaattt
     6061 aggagctgga ttgttaccct taaactgtag atgggcaaca aactgaccct ctcccttaaa
     6121 ctgatgaggg tacatccgag ccgtttctgg caggtcaata ccagctacca ttccattgat
     6181 atgctctact ggcaacaagt caaaatcata ctcttccagc aaccaattga caatctcttc
     6241 gttttcctcg ggtgcccagg tacaggtcga ataaaccaga cgaccacctt cagctaacat
     6301 ggtcactgca tcctccagaa tttctctttg caagctagca cattgactcg gataatctaa
     6361 gctccaatag tccatagcat caggttgctt acgaaacatt ccttcaccag agcaaggggc
     6421 atcaagaacg attaagtcaa aatagccttt aaagaccttg accaagcggt cggcagattc
     6481 attggtcacc acgacatttg tcgctccaaa acgctccatg ttttcaacca aaatcttagc
     6541 ccgtttgctt gaaatttcat tggaaacaag tagcccctcc cctgctagat aggctgccag
     6601 ttgagttgat ttgccccccg gtgcagcagc caagtccaag accttcatac caggactggg
     6661 ttgggctact tgagccacca tttgagcagc aggttcttgc gaataaacta aacctgtagc
     6721 atgctcaggc gatttccctg aaaccttccc atagtggccc caaggggttt gagtaatggc
     6781 atcagaaaag gaaagttgct cttcttttaa gggattgacc cgaaaggccg aaaccgcttc
     6841 ctcctcaaaa gaggcaagaa aatctcttgc ctcatctcct agtatttctt tatatttttc
     6901 aacaaatcct tctggaaatt tcatttaagt tcttttcctt tcgtaaatat aggactgaat
     6961 ttcctcctgc atctcaagag gcaccatcat gaccggctgt ctggtttgaa aatcaggagc
     7021 ttcaccaaaa agggtcacaa cccgatagcc cagactttcc cctaaaatac tagctgcggc
     7081 ataatcccat ggttgcagat aggtgagata ggtcaacaaa tgccctgaca aaatcttggc
     7141 aaaactaatg gccgcacttc catagacacg aacaccaaga accgctcggc tcaaatcagc
     7201 cagcccccat tcattggttt ccagcatacc actattccct gcaatgagaa aatctccaag
     7261 tggtttagtt ttaaaaggag ctaggggcct atcatttaga caaactggaa attccccacc
     7321 accgtggtaa caatcccctt tgaccacatc ataaatcaga ccaaactgtc cctgaccatt
     7381 ttcaaaataa gtcatcataa cagcaaaatc ttcctgctgg gctacaaaat tattggtacc
     7441 atcaatggga tcaatgaccc aaaccttgcc ctcttgaacc gaggctcgca gacaaccttc
     7501 ttcagcacaa atcttatcct caggataacg ggacaaaatc tcaccaacca agagttcctg
     7561 aacttctttg tccagtctgg tcaccaaatc tgttggagag gacttggttt caacacgcaa
     7621 gtcttcctgc atatggtcaa gaatgtactg gcctgctttc ttaacaagct ctttagcaaa
     7681 ttcaaattta ctttccaaga gaaatctttc cttccccttt ttctttggca gcctgaactg
     7741 ctcggtaaag tgaatagcca ctaaccgttt caaattctcg tcccaaacgt ttctcttcgc
     7801 tcttgcttgg cacgaccgct ttgaatccct tataggagtc cagtaacttt ttagcctcta
     7861 ctttgccttc ataggcagct tcaacatcat taaaaaaaga aagcactgaa gcaagttctt
     7921 cagtgctcca cgacaaatct agtgggtaac tatactgttt gttcattaac taataccagc
     7981 tctcattctt gcttctttta gttcttgctt acgataacta cgagggagaa aagcacgaat
     8041 ctcatcttca ttaaaaccga tttgcatacg cttggcatca ataataattg gacgacgcaa
     8101 aagactagga tactgctcaa tcaaatgaag caattccgat accgaaatac tctctacatc
     8161 aatattcaat ttttgaaaaa tttttgaacg agttgaaatg atgtcatcag taccattttc
     8221 ggtcaaggaa aggatgtgtt gcaattcttt tcttgttaaa ggactggtca taatattgtg
     8281 ttccacaaag ggaaccttat gtttttctaa ccaggcctta gccttacgac atgatgtaca
     8341 gctcggtgat agaaatagtg taatcatgct tttctcttct tatctatact ttgctacttc
     8401 tattatacaa aaaaataaag cgcttgacta gggattttta gaaaaaaagc ctattttttc
     8461 aagaaaaata ggctttttgc gaacgattga cacaattgga tttggttaat tcactcttaa
     8521 cgatggtttt aaacgatata tatttttata tatgtaaatt aaaaacatct ttcctttcac
     8581 ttcctacgac ttttcagata cagatagcca aagaagtttt catagagggc aaaaaagagg
     8641 aggaaggcat gaagaaagaa ggtctctggc aaaatcataa taacaggatc cttggctgga
     8701 tcaaaaagcc aggtatcatc tcccacaaag agaatttgat ggaaaagagt aaagaattgg
     8761 tcaaaaccaa tcaaaactcc cccaagtcca atcatcacag gtaagactac tagagccagg
     8821 agactttttc gataaagaga caaaaagtcc tttttcacaa tcctattgac aaagacatag
     8881 aaacttggca gtgtcactag agctactagc tgaaccaaat gaaagagatt cttgaccact
     8941 gcgaaatggt gcagaccagc tgctgacgaa cgaaaatcag gcatctgtaa gacctgacta
     9001 aaaggattgg tcagataatt catcaagata tgaaaattgt attgaatggt ttctggtttt
     9061 agatagactc gattcgttaa gtttagccac tgaatctcca taggatagaa aatccaagcc
     9121 agataaatgg tcagaaggat tgagagggag aggagaaaga gcatagagcc ccaaaaggtc
     9181 aatttagttt tcatcaaaat cccactccgc aaggctagaa accacatgtg tcggtgcgat
     9241 tggcaggcca gctacttctt ctgccttagt aaaacctgtc gtcaccaaga gcgttggaat
     9301 gccattgtca atcccagccc ggatatcagt caaataattg tccccaacca tgattaactc
     9361 ttcacgttcc aaacctaagt gctcaaccgc cttgtccata atgatggcat ttggttttcc
     9421 gatataaacc ggcttcactc gtgtcgctac ttcaagcagc gtaatcagtg agccagcacc
     9481 tggcaaaaga ccacgttccg tcgggatgtt gaggtcagga ttggttccga taaaatgggc
     9541 acccttttga atagcaagag ttgctgtggc aaatttttca tagtcgactt gccaatccag
     9601 accaactacc acgtaggcag ggttttcctt gtcttccaca taaccagccg ccttgatggc
     9661 ttccttgagt cctgcttctc cgacgacata gacagtcttt tcaagcccca aatcattcat
     9721 atagtcgatg gttgccaaag tcgctgtgta gacagtcgat agaggcgtat cgatattaaa
     9781 attctgagcc aacatctcct taacactctc tggagtgcgg gttgtattgt tggttacaaa
     9841 gagataggga atgtcccgct tttgcaattc atgaacaaaa gtctctccag cagggattcg
     9901 gtctttcccc ttataaatgg ttccgtctaa atcaattaaa tagcctttat atttcatcta
     9961 tttctcccta aacctttttt atttcttgcc aagtaatgat tgcttgggca ttgataaccc
    10021 catcacttgt aatttcatgc ttgctttcca gtccagtccg ttcaacagcc gatgtaatca
    10081 ccccacctgg tcgaacttcc ttgacatact tgaggttgat tttcttggga atatagtggg
    10141 tcaaaaaatc cgctcccatg acctcaaaaa tccagtccaa gtatttactg ttattgacat
    10201 gaccattcat atccaagtcg taaaaacgaa catggtaatc cttgctgatc ggctcttcca
    10261 aggattcata cttcggtcca cggataagtt ttttatcaaa atcagactgg taaggagcca
    10321 caatctcagg ttcaacagca tggacttttc gactgtcgcg gtccatgaga acaaaggtcg
    10381 ccatcatatg gatgagctcc tgccccgctt cattataaat agtaaagcga cggtagcaaa
    10441 aaagtcgatt gtagctcaag gcttccgttt cgatggtaat ttcttccgca aaacgaggca
    10501 aacgaaccac ctcaatatca tattctgcga taatccagac cagattatag tcttccaaaa
    10561 tggccttatc actaactccc agttcaatcg attgcatccc tgaaacttgc agtgacagca
    10621 aaatcacatc tggaagtttg atatgaccgt tcatatcagc catatcaaaa ggaattttca
    10681 ttttcatttg ataagttaag cacatgatcc tactccaaaa taaatcgttc tgctacagta
    10741 tctcccaaaa agagacctct ctttgtcatg cgaacgtggt caccctcaat ctgcatgagg
    10801 ccttgttgaa ccaaatctct gacaatttct ccataaagtc cagcaaaaga ctgtccaaat
    10861 ttttcctcaa atcgcgccat ggaaaccccg gatttcttgc ggagtcccaa gaacatttct
    10921 tcttccattt gctccttttg actcaggtga tcttctgtaa tacaagcatt gccttcctca
    10981 accgcactga gataatgacg aatgggacca tgatttttat agcgtactcc attgacataa
    11041 ccagatgccc ctgcaccaat accatagtat tcagcattgt cccagtacat gagattgtga
    11101 cgactttcaa agccgggttt ggagaaatta gaaatctcat aatgctcaaa acccgctcgc
    11161 tccagctctg caatgatgta ctcaaacatc tccgcttcta gttcctcctt aggcagaggc
    11221 aatttcccac gtcgcatccg gttcataaag accgtatggt tttctaaaat caaactatac
    11281 aaactcatgt ggggaatatc cagtccaatg gctttagcca cattttcctt tacttgctcc
    11341 atggtctgac caggcagagc ataaatcaaa tcaatggaga tgttgtcaaa accagccagt
    11401 ttcaggcgat cgatattttc ataaatatcc ttctccaaat gactgcgccc aatctttttc
    11461 aacatcttat catcaaaggt ctggacacct agcgaaacac gattgacagc cgaatttttc
    11521 aaaacagcta tcttatccgc atccaaatcg cctggattgg cttcaatggt caactcttcc
    11581 aagacagaca agtccaagtt tttagtcaag ccattcagta acacctccag ttgcggagcc
    11641 gacagggctg tcggtgttcc accaccgata taaagggttg acaacttttc aatatcataa
    11701 gaacgaaact cttccagcag atgctctaaa tagctgtcga ctggctgatt tttgatgaag
    11761 acctttgaaa aatcacaata ataacaaatc tgggtacaaa atgggatgtg cacataggct
    11821 gacgttggtt ttttctgcat agtaattatt ataccacaaa gactagattc cagataaaaa
    11881 tcaccatccc cagatacata gtccgtccgg agatggtgat ggtttattct tctgttatat
    11941 caatcacaat ctcttctgag tcatcaagag cttcggcttt ttcttgccat tgctccttga
    12001 gattatttaa ttgatttttt gatgcttctg tcgcttgaaa agcataggat ttagcttgag
    12061 caagtatact gtccacagtg atttcacctg actcaacctg ttcttttgtt ttcagaacaa
    12121 aatctgtagc ctgctcctta acttctgtca gtttttcaca gacttgctcc ttggcatact
    12181 ccggatcttc tctcaaatca tctagaaaat cttgagcctg actgcaaact tgtttgccct
    12241 tatcacttgt taaaaacaag gcaagagctg cacctgaaac ggttcctaaa aggattgagg
    12301 ataatttacc cataaggatt ctcctttttt attttttgaa aaatttactt gcaagacgaa
    12361 gagctgacag acttgcacca gtcttgagtg tttttgaacc agctgatgaa gctttcttgc
    12421 tcaagacacg cgcatggtca ttgaggtctg aaacagatag agataaatct gcaacagcac
    12481 tgaagagtgg atcaatcgta gccaccttga cattgatatc atctgccaag acattgacct
    12541 tagccaacaa ctcattggtg tgatgcaagg tcacatccac atctgaagtc aaggttttaa
    12601 tcgtcttttc tgtttcatcg atgacacgac caagcttttg tacagtaatg atcagataga
    12661 ccaaaaagac aatcaaagct agggcaacaa gaatatatgc aacttctaac atttagtttt
    12721 cctcctctgt aatatagtaa ggggccttct ttcgattttg ataaataacg atcattatac
    12781 cgagaccgat aaagacaact gacagccatt gggacactcg aaggctgaag aacatgagac
    12841 tatctgttcg cataccttcg atgaccatac gaccgaaacc ataccaaatc aagtaaaagg
    12901 ccgtgatatg acctcgtctg agactcttcc atttccgtct aaaaatcaga atcaaggcaa
    12961 agccaagcag attccataga gactcataaa ggaaagtcgg ttgacggtag ctcccctcaa
    13021 tatacatctg gtcacggata aagccaggta gataatccag attatccact gttgcaccat
    13081 aagcttcttg gttaaagaaa ttaccccaac gccccaaact ttgagcaatc ataacgctag
    13141 gcgccgcaat atctagaaaa tcccaagtat tgatgagttt acggtcagca aagatataga
    13201 gcacaagagc cccagttatc aaaccaccgt aaatggccaa accaccattc caaatggcaa
    13261 aaatctctcc taaattctga ctatagtaat caaatcggaa aataacatag tagagacgag
    13321 ctcctaaaat agccaaggga aaggctacta agataaaatc taaaatatcg tctggtatga
    13381 tcttctttct aggtgcttct ttcatggtca aataaaccgc aagaatcaag cctgtcacaa
    13441 tacataaggc ataccaacga atggctaggg gtcctagttg aatagcaatt ggatcaagca
    13501 ttttgcacct catttcgagc tatgagactt gtcagtcgtt cgtcgaacaa acgggtagca
    13561 tcaaagccca tttccttggc acgataattc atggcagctg cctcaatcac aacagagata
    13621 ttacgacctg ttttaactgg aatacgaata cgaggaatgg ctacgccaga aacttcaagt
    13681 tcctctgcat tgtttccaag acgatcaaag gtcttatgcg tatcgtaatt ttccaaatag
    13741 acagcaagct gaacctgtga agaatccttg acagcactcg caccgtagag actcataaca
    13801 tcgataatac caaccccacg aatttcaatc aagtgtttca aaatttcagc tggttcaccc
    13861 cagagagtaa tctcatcctt ggcaaagata tcgacacggt catcggctac caaacggtga
    13921 ccacgtttga caagctcaag acctgtctcg ctcttaccaa ttccactatc tccctgaatc
    13981 aagacgccca tcccataaat atccatcaag acaccgtgca cactggtacg ttctgccaaa
    14041 cgagaatcca gatagctaga taactctcca gataaacgac tggtagctgc acggctggtt
    14101 aaaatagcaa tcttacattc tctagcagcc tttaacatct cctctggaac caccaaacca
    14161 cgggcaacaa tgactgctgg tgtctcaggt agaaacattt ttttcaaaac ttcataacgg
    14221 ctgttggaag gcatgctgat cagataagac cactccttca tccccaaaag ttgaatccgc
    14281 tctggtgtat agtagtcaaa atagcctgtc atttcaagac caggtcgcgt aatatccgct
    14341 gtattgattt ccttttcaag caattctggt tcaccataga caatatctag tctaagcttt
    14401 tcaatcactt cttttactaa aaccgacatc atttcctcct tcaaatgaat tcttctctct
    14461 attatatcaa attgtaggag gaagtttcag atttttgcag gctttggagt atttatttaa
    14521 aagaaaaagg actagatttc tctagccttt catttcaatt agaatttttt acgtttacga
    14581 gctacttctg atttacgttt acgttttaca gaaggttttt catagaattc acgtttgcgt
    14641 gtttcttgaa gagtaccagc tttagtaacc gcacgtttga aacggcgaag tgcgtcgtca
    14701 agagattcat tcttacgtac tactgtttta gacatttttt tcactccctt caagttcaat
    14761 atctatttca ttctacacgt aaaaggaata attgtcaagc aaaaatgcgg tttaattttg
    14821 attttttctt ccatttatac aagagttgaa agcgttcaat agaacattgc ttttttattt
    14881 ttcgagtaag ctaagcgctt cagcatctgc gatgatggtt acatcagggt gattttggag
    14941 gctacttgca ggtaggttct cagtcactgg gccagatact gttccggcaa tggcttctgc
    15001 tttcgactca ccataagcaa aaagaataat agacttggca tccaaaatgt ttttaatccc
    15061 cattgaaata gcttgggttg ggacgtcttc aatcttggca aagaagcgag cattggcttc
    15121 gatagtagac tggtcaagtt ctactagatg cgtttgactg tcaaatggag taccaggctc
    15181 attaaagccg atatgcccat tgcgaccgat tcccaagatt tggaggtcaa ctggatggtc
    15241 agccaaaatt tggttgtagc gttcaacttc agcttcagca ttgtccttaa caccacgagg
    15301 caagaaactt tctttaaatg gtttttggtt gaacaagttt tcttgcatga agtaacgata
    15361 agactgtgga ttgtccccat caaggcccac atactcatca aggttgacac tggttagatt
    15421 tgaaaaatca aggtcactct caacaatttc cttgtaaaat tcaagtgggc tacttcctgt
    15481 cgcaagtcct agagtttgag ctccattagc caatttttcc ttcaaaatct caaaagcaac
    15541 ttttccacct tgaacttggt tttcaacttt aataactttc atcttttcat cctccatttc
    15601 tgtctatatt tattatatgg tatagaccaa tttttgtcaa gtgaaaacga tttaatttct
    15661 aaaaaaagag cgtccaaact tgaaacgtgt tataatggat aggattagaa cttagaaaga
    15721 atgaacaaat atatgaatac agctgatttt gatttccact tgccagagga attgattgcc
    15781 caaacgcccc ttgaaaaacg tgatgcctcc aaactcctca tcgtcaaccg tgagacagga
    15841 gaaatgcaag ataaacattt ccactctatt attgatatgc tggaacctgg tgatgccctt
    15901 gtcatgaacg acacccgagt tctccctgcc cgcctctatg gtcaaaaagt ggagacagga
    15961 ggtcatgtgg aacttctcct ccttaagaac actagtggag acgagtggga agttctggct
    16021 aaacctgcca aacgcctcaa ggtcggtact cgtatcagct ttggtgatgg ccgcctcagc
    16081 gctgtcgtta cagaagaatt gacccacggg ggacgcattg tccgctttga ataccaagga
    16141 attttcctag aagtcttgga aagtctggga gaaatgcctc tgccacctta tatccacgaa
    16201 aaattagatg accgtgaacg ttatcaaacc gtctacgcca aggaaagcgg ctctgctgca
    16261 gcaccgactg ctggtcttca cttcaccaaa gaactgctgg cagaaatcca agctaagggt
    16321 gttcatctag tctatctgac tctccatgtc ggactcggaa cctttagacc tgtttctgtg
    16381 gataatctgg acgaacacga aatgcactca gagttctatc aactttctga ggaagctgct
    16441 gccacccttc gctctgtcaa agaaaatggt ggtcgcgtca tcgctgtcgg aaccacttct
    16501 atccgcacct tggaaactat tggttccaag tttgatgggc aaatccaagc agattctggt
    16561 tggaccaata tctttatcaa acctgggtat gagtggaaga tcgtggatgc cttctcaacc
    16621 aacttccatc tgccaaaatc aactctggtc atgttggttt ctgcctttgc aggccgtgaa
    16681 ttagtcttag atgcctacca ccatgccatc caagaacact accgcttctt cagttttggt
    16741 gacgccatgt ttatttattg agaaagaatt tctctaattc ttctaatacc aataaatcgc
    16801 taagatatta tttcaaagaa catctacaat tgaaactcta gctagctgta gaagacgcct
    16861 agtacattga aattaaaatg cttcccccta gcttcgaaaa tattgccata gattgcgttg
    16921 actctccaaa ttgattcatc tatattttat ttcagcttcc tatattttct tcgctgtttg
    16981 taaatcaaaa tgcaagacac atgagtagca ccatatttgt tactcttatc tgtcctctca
    17041 agagactatt atgagttatt tcagaatcat tcactacttt gaccctgact ctccttagtc
    17101 tcaaaatcaa agacttatac tcttcaaaaa tctcttcaaa ccgcgtcaac gtcaccttgg
    17161 attatatatg tgactgactt cgtcagttct atctacaacc tcaaagcagt actttgagca
    17221 acctgcgact agttttctag tttgctcttt gattttcatt gagtattaaa caaaaagtga
    17281 acaaatctga attctaatgt acagaagact aggcttgttc acttttttat agtcgctata
    17341 agatgacctt atctatagct ttttatatat aattatatat tcagacatac tattatcaat
    17401 tttgtcgcag ggaggaatct gttaacgcac ccattcacca ttatcattga ctctatagcc
    17461 atctatactt gtattgaccg ctaactcacc cgatgcattt acataatacc atttaccacc
    17521 aacttggaac cattgattga ctttcataga accgttgctg ttgaggtagt accatgaacc
    17581 attaacttgt acccaacctg ttgccatgga accatcagta ttataaaaat accacatacc
    17641 attttcttgt ttccagtctg ttgttggagc aactgcttta gctggttcta ctgctacatc
    17701 tgttccttgg ttagatgtaa cagatacagg atacgaagga atagatgatt gctcaggaac
    17761 aacaactttt tcaggttctc tcgtccctct ccttatacgt ctttttacca tctctttagt
    17821 aatttgacga gaagtagttt cttcaattat tccatcacgt tcatctacag tatagattgt
    17881 agtaagagta atttaccaat ttctcctact tcttctactt cttgactttt atcaagagtt
    17941 gggccatcga gatattctgt ttcgattgga atttcttgga caagaacttg gggcttggtt
    18001 ccttttttaa caactcttgt ttgagagtct ttttttgact taaagtactc tcagttactt
    18061 gtccactctt tccatctaca ttataagtta tcgttgtaac tgttttccca ttctttccta
    18121 gagtaatctc ttgctcctgt cctgcagaaa ggtcattgtc tgcttcatat ttagtagcaa
    18181 atggaacaag aacttcttca atcttgcttt tagctggaac tttgataact gtatccgtgg
    18241 cttcttttct atcaacagta acctgttcgg taacataacc agtctctgga ttaacatcgt
    18301 aggtccttgt cgtagttaca tagccatcct ctccgtcaat tgtaacagga ttttcactac
    18361 ggtcttttgt ttcatctttt tcaaaacgaa ttcgcgtact tgaaattttc ttggttacta
    18421 ccttaggttt agtcgctact tttacaataa tatccccatt gtcagcgtca tcatactcta
    18481 ttccctcttc tttatctcta gtatcatctc tgacatattg aatcccatca gcagcatgag
    18541 caaaacttgt attcagattc ctcctaaaaa taaagttagc ccgattatcg cagaaccaaa
    18601 aatctttccg agtttacgta ttgcataacg cttattagta ttagattttg ccattacatc
    18661 ctacttctag tatagcatct tttctatcaa acgttaaaca atatacttta tatataaaat
    18721 agacttagaa tgatatattg attattgaac taacacttta actatatcgt aatcaatctc
    18781 atatataaag gattgcagac atctgatcta aatacatgct aatatattta gatacaaaca
    18841 ttccaacttg ataattttca ctcatctttc aacattcctt acacaacgat gcagtataaa
    18901 tagaatagtt ttctcatcag aatgagacta ttttaatatt agataatttg atagaaaaac
    18961 ataaataaaa ttggttgaat ttaattccct aactcgatag aggattctat tttaaattta
    19021 acaacagcta ggattacact ataaaaagta cattaccacc tgttcggctg gagaatctag
    19081 tcgattagct ccctcttagt tacaagctta aagcgatttt ttaactaata ctaaaccaga
    19141 ttaaagaagg aactagaaca tctgtcttgt actaatcaat atgctatttt aaaattgcaa
    19201 ataaacttgg aatgctaatt attatattta caataaatag ttgacttcga tagaaacaga
    19261 aaatagaatt caacattctt ccctacttat accataataa ataagatccg caagatagcc
    19321 ttttctctct acaccattaa caatggtttg tagataggac attccagcct tttccatgac
    19381 acgacctgaa gctgggttga gactggcata acgtgctctg accttttgaa aacctgcttg
    19441 agtaaaacaa aagtccaaga cagctttcaa ggcctctgtc atcataccat gtccccagta
    19501 agccttgcct agcacatagc cgatttcaca gcttaaatca gcctcgtcta tcttaacgat
    19561 gctgatatct cctatcactt gctctgggtt ttcttttaga caaatggccc atttgtaata
    19621 gttgagatta gtataggaag caacccaatt gcgaatcgag tttcgagtga tttcgacatc
    19681 aggatgggga tcccaggtaa cataggtcag attctcagtg gatgaagccc aattttgaaa
    19741 catggcttct gcatcactct ccacaaatct tcttaaaatc aaacgttctg tctgtaatat
    19801 ttgcgtaccg atagatttca tgatgctacc tcacttcctt atttctatac tcgtagtaag
    19861 aaggctcaac tcagtaacag ctagaaaacg agtttgtctt ctaatttctt gacgcagttg
    19921 ctgcctggtt tgaaatgcgc tctagcaagc tttttcaaac ctagtcatcg ttgcaggggt
    19981 aagacgatga aatcgaactc ttcgagttac tgaatctgcc tcattttctt atttctaaac
    20041 tcggggttca agatccccca gaccttctct cactttctta tttcaagatg agaggctgaa
    20101 aacctccact ggaggttttt cttgcttttt aatttcaggg caagaagcta aaaaggtccc
    20161 ccggaccttc ttcgctttct catttctagg ctcagggtaa aaacctccag tggaggtttt
    20221 tactcccaaa agtcatcaaa tacagtgatg ggtaagtggc gtttgtgttg gcctttgtgc
    20281 caccagtttt caatggtcgc ttgagcttct gggctgattg ttttgccttc taggtagtcg
    20341 tcaatctctg cgtaggtgac tccaagtgcg acttcgtcag ctaggcctgg tttatcttct
    20401 tctaggtctg ccgttgggat tttttcataa agggctggct ctgcgccaag tttctgcaag
    20461 agctgttttc cttggcgttt attgaggcgg taaagaggga gaatatccgc accgccgtca
    20521 ccaaacttgg taaagaaacc tgtgatattt tccgcggcgt ggtctgttcc aatgaccgct
    20581 ccgctatggg aaccagcaag ggcatactga gcaatcatac ggcaacgtgc cttgatattc
    20641 cccttgttga agtctgaaac aggactacct gtcgcttcaa ctgcagctgt catggcatca
    20701 gctgattcct tgatattcac aaccaagctg acatctggct ggatgaaggc tagggctttt
    20761 tgagcatctg cttcatcagc ttgcactccg tatggcaggc ggacagcgat aaatttgtag
    20821 ctatcgtctc ccgtttcagc tcgcagttct tccatagcta attgcgccaa acgtcctgcc
    20881 aaggttgagt cttgtccccc agaaatccct agtacaaagg tttttaggaa gggatgtttt
    20941 ttcagatatc tttttaagaa atcaatagaa cgacggattt cttcctgggc atcaatcact
    21001 ggtttgacac ccagctcttg gataatcgtt tcttgcaaac tcattcttct tctccttcac
    21061 caagggcttc cttgcgcatc ttatcaatca agtccatctt atcttgccat acatcacgcg
    21121 ccaaatccac tggatagtgc tgcggattga gcacacgctt atactcatcc cacaacttgt
    21181 caaattcctt acgggcataa tcctgaatgt cagtcaaact aggcaagttg taaactaata
    21241 ttccttcttt gaagatatcc accaagagag gaacggcatc aaaattacga accgtcttct
    21301 tgatgtatgt ataggtcgga tggaacatct tgatttctgt catgtcgcta atatccacac
    21361 catcataagt gatgtagtca ccttctgact tgcctttttc acgactggta atgcgccaca
    21421 cctgcttctt acctggcgtc gacacttttt ccgcattatt agacagctta atcgtattgc
    21481 gcatctggcc gttttcatct tcgattgcaa caatcttgta aaccgcccca agagccggct
    21541 ggtcataggc tgtaatcagc ttggtaccca caccccagac atcaatcttg gccttttgca
    21601 tcttgaggtt aaggatggta ttttcatcta gatcattaga agcataaatc ttagcctctg
    21661 gaaatccagc ctcgtccagt tgctgacgga ctttcttaga aatgtaggca atatccccag
    21721 agtcaatccg cacacccata aagttaatct gatcacccag ctcacgcgcc acctgaatgg
    21781 cagctggtac accgatgcga agggtatcat aggtatccac aagaaagaca caatttttgt
    21841 gggtcgcagc gtaagccttg aaagcctcat agtcattgcc ataaacctgt accaaggcat
    21901 gggcatgggt tcccaaaaca ggaatgtcaa agagcttacc cgcacgcacg ttgctggttc
    21961 cattggcgcc accaatcacc gctgcgcgtg ttccccagat ggccgcatcc atttcttgag
    22021 cccgacgtgt cccaaactcc atcaagggtt catcttcgat aaccgaacga atacgagctg
    22081 ccttcgtcgc caccaaggtc tggtagttga cgatgttcaa aagagccgtt tcgaccaact
    22141 gacattgggc tagaggtcct tccacctgca caatcggttc attagcaaaa accaaatccc
    22201 cttcttgggc agaacgaacg gtcaactcca acttgaaatt gcgaaggtaa tccaagaacg
    22261 ccccatgata accaagcgac tccaaatagg ctatatcact atctgaaaaa cgcaagtctt
    22321 caagatagtt cacaattctt tccaaacctg caaaaaccgc atagccgttc ttaaaaggct
    22381 gttggcggaa atacacctca aagaccgcct tcttattgta aatcccttga tcaaagtaaa
    22441 cctgcatcat gttgatctgg tacaagtccg tgtgcaatgt caaactatca tctggataca
    22501 tacttttcct acttccttag ctagaaaccc atgaaaattt tcaagaactt tcatgtattc
    22561 caataaatta gtactattat atcacatttt agctggattg agaaaagagt aacaagctat
    22621 tctccactct ccaattcatc catatcttgt tcaaattttt tctgagccca ttcgccatag
    22681 ctcttaagac caagattgcc aataaagacc cacggaaggt aaatgacata agtaatgacc
    22741 caagcagaca ggtatttaaa attcaaagga ttgtgctgat aaatttctat gttgaattga
    22801 taattctgca acatcaaaag agccgtaata gccaaggtta ggaaaaaaca acccaaaatc
    22861 gtaaaatgaa aacgactata gtaggtcact cccagataac gggcacgatt gaaaaagtaa
    22921 aatgtcccta tgatgataac gattagcagc atattagaat taaaaaggct tggtgctaat
    22981 actgaaatga tataagatag gagcgacaaa gcaatgcaga tatagaaact ttcagagccc
    23041 gctttattga acagttgttc ttctctttcg tctagtaatt gataataata aaatctattt
    23101 ttcatcttct tcctcccaaa atagttggtc tagggttttc cctaaacatc tgcaaataga
    23161 ctggcagagc gagagactgg gattgtattt tcccgcctct atcaaaccaa tagtctggcg
    23221 tgtcaccccg acagcctctg ccagttgacc ttgtgttaaa tcacgctcta cccgagctaa
    23281 ttttaatttt aaatttttag ccaccttcgt cctccttata gttttaatac tcatctactc
    23341 ttaaaaaatc caaaaccaac acaagctatc agctatttat tataacccca ttaatttgca
    23401 ctccagtatt atcttcagcc gaaaatcagt attttaggtt gtttgaaata aacttcctag
    23461 tttactcttt tatttttatt tagtattagc tttagtagga tcttttaaac gagtttttat
    23521 tgcttatatt tcttgtttag tttagttggt ttcatatcac tattatataa tgcttttgga
    23581 aatgagccta gtataagtca aaaacgtgtt gaatagtgaa agcaggagca taccaagatg
    23641 actgattttt tactgtattt taggtatgca aaataatact aaatgaaact aaaatgaata
    23701 cttcttgctt ttcttgagag tgaaaaagta caataaaatg attgtagtga ttagcacaag
    23761 atactgacta atagacatcg tatttgaagc cacaaataca ggtaaagaat aaaaaggaag
    23821 aagttcaatt gctttgataa acaaatcatt actagtattc atcatcatcg tatttaatat
    23881 attagggata aagagtagta aagctactaa aataccaaat accaagccac ttttcaattt
    23941 gtataaatag aaaatgtagg ataatagaaa ataaaacagg actaacaaaa ttgtattgac
    24001 tacaatagct actataaaat ctaatcctaa atcacctgga atacccaatt tacttactat
    24061 gactagtaac acaatgccat tccaagcata aataatggaa actgagatgg catatagaaa
    24121 attagacaag acgtaaatca tccgattctt ctgttgatta taaaacaaac ttaccgtatt
    24181 gtgggaaaaa tctctactaa tgactcgatt agcatgtata acagccatta acataccgag
    24241 catggcataa aagtttgaaa tatgcttaat accaagcgat gtcccttgag tctttagaat
    24301 aaagaatgta agcaatatcg gcggtataaa cgagataagc aaactgagat atattacgcc
    24361 tgatgaatat aaatttctta tactttcctt aagaaaattc aatttattta atttcatgat
    24421 tataaccttc ctttattttg aacgatttag gtaaatatct ttaagcgtct cttttttagt
    24481 ttcaaaatca actactttaa tagagttttc gttaaaaaat ttaaaaagct cactactttg
    24541 aatattccca gacatagtaa ttctcagtcc ctcttcctga acaatatctc caaattcttg
    24601 tttggtaatg aaaatgtctc tatctgtagc tgatgaaaaa gctatctcaa acaagaaatt
    24661 atgactatct tttcctactt tttgaaatgt caaaagcccg ctctccaaga aaagaaccct
    24721 ctcacaaatt tcttcaatat cttctaattt atgactcgat attaaaattc ccacattttc
    24781 atgtaaagct aaatttttta gaaccgctaa aactatttgt gatgattcaa tatctaaacc
    24841 attagtcggt tcatctaaaa tcaatatatc aggttccgta acgagagtta gaagcaaagc
    24901 taatttttgt tttgtaccca aagaataggt ctttactttt ttattaatag actgagtcaa
    24961 atctaactct tggatcaaac ttctaaatct ttcttgattg tagtcaacac catataaatt
    25021 tgacaaatat tttaaattct ctaaacctgt tttagataaa aataattttg gttcttcgat
    25081 taaataccca atattatcac tgcttataat attacctgaa gtcggttgat tattctgagc
    25141 aagaattttc atcaacatac tcttaccaac tccatttctc cctactagac caacaatttc
    25201 acccttgtta agtgcaaaat ttatgtcttt gagaatgatt gaatgaccat aatgcttact
    25261 tacatttata acttacattt tttattcctc cattaatatt catggtttgc tctttgattc
    25321 ttattgagtt taaaaagttg cgcctctttt taacaacatt atatcatata tctaacttaa
    25381 tgcaatatat aaatatcatt ttgtaaaatt ttattaacaa aaaactctct acaacaaaat
    25441 aatagagagt tttctgctta ttaatttttt tcaaaatatc taagacatca cttctttgtt
    25501 taatttgata ggctttgata cctaagctcc gactttatca cttaaaaaat aaaaaaggac
    25561 agctaatctc ttgcgcatca agggatttcg ccattcttta atctcgttta agtgttgtat
    25621 atggagcttt gcataatttt ttaaaactaa acaactacat taagcacgaa ctgtgacgct
    25681 ggttccatct tccttataaa
//
LOCUS       DAVKMU010000026        25347 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKMU010000026 DAVKMU010000000
VERSION     DAVKMU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.026"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..198)
                     /locus_tag="VW343_001271"
     CDS             complement(<1..198)
                     /locus_tag="VW343_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755820.1"
                     /translation="MLIVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKK"
     gene            complement(206..577)
                     /locus_tag="VW343_001272"
     CDS             complement(206..577)
                     /locus_tag="VW343_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU9755821.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            1335..1655
                     /locus_tag="VW343_001273"
     CDS             1335..1655
                     /locus_tag="VW343_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9755822.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILFHLKKEVEIYYQ
                     AKER"
     gene            complement(1944..2414)
                     /locus_tag="VW343_001274"
     CDS             complement(1944..2414)
                     /locus_tag="VW343_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU9755823.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(2380..3186)
                     /locus_tag="VW343_001275"
     CDS             complement(2380..3186)
                     /locus_tag="VW343_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9755824.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(3402..4043)
                     /locus_tag="VW343_001276"
     CDS             complement(3402..4043)
                     /locus_tag="VW343_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755825.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(4040..6148)
                     /locus_tag="VW343_001277"
     CDS             complement(4040..6148)
                     /locus_tag="VW343_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9755826.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(6200..6487)
                     /locus_tag="VW343_001278"
     CDS             complement(6200..6487)
                     /locus_tag="VW343_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9755827.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            6912..7232
                     /locus_tag="VW343_001279"
     CDS             6912..7232
                     /locus_tag="VW343_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9755828.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICTDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            complement(7285..7857)
                     /locus_tag="VW343_001280"
     CDS             complement(7285..7857)
                     /locus_tag="VW343_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9755829.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            8099..8770
                     /gene="sdaAB"
                     /locus_tag="VW343_001281"
     CDS             8099..8770
                     /gene="sdaAB"
                     /locus_tag="VW343_001281"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9755830.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            8779..9651
                     /gene="sdaAA"
                     /locus_tag="VW343_001282"
     CDS             8779..9651
                     /gene="sdaAA"
                     /locus_tag="VW343_001282"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9755831.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            9673..10305
                     /locus_tag="VW343_001283"
     CDS             9673..10305
                     /locus_tag="VW343_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9755832.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            10355..10432
                     /locus_tag="VW343_001284"
                     /pseudo
     CDS             10355..10432
                     /locus_tag="VW343_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(10698..12548)
                     /locus_tag="VW343_001285"
     CDS             complement(10698..12548)
                     /locus_tag="VW343_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9755833.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(12590..13747)
                     /locus_tag="VW343_001286"
     CDS             complement(12590..13747)
                     /locus_tag="VW343_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9755834.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKEDIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINQNML"
     gene            13805..14971
                     /locus_tag="VW343_001287"
     CDS             13805..14971
                     /locus_tag="VW343_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9755835.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            15110..15436
                     /locus_tag="VW343_001288"
     CDS             15110..15436
                     /locus_tag="VW343_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9755836.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            15423..16016
                     /locus_tag="VW343_001289"
     CDS             15423..16016
                     /locus_tag="VW343_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9755837.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYATRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            16009..16920
                     /locus_tag="VW343_001290"
     CDS             16009..16920
                     /locus_tag="VW343_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9755838.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            16983..18047
                     /locus_tag="VW343_001291"
     CDS             16983..18047
                     /locus_tag="VW343_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755839.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVPQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(18225..19085)
                     /locus_tag="VW343_001292"
     CDS             complement(18225..19085)
                     /locus_tag="VW343_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9755840.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFERVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(19360..20346)
                     /locus_tag="VW343_001293"
     CDS             complement(19360..20346)
                     /locus_tag="VW343_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9755841.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEEREQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(20618..22093)
                     /locus_tag="VW343_001294"
     CDS             complement(20618..22093)
                     /locus_tag="VW343_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9755842.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(22303..23226)
                     /locus_tag="VW343_001295"
     CDS             complement(22303..23226)
                     /locus_tag="VW343_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9755843.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(23240..24169)
                     /locus_tag="VW343_001296"
     CDS             complement(23240..24169)
                     /locus_tag="VW343_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9755844.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGTLWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(24488..24708)
                     /locus_tag="VW343_001297"
                     /pseudo
     CDS             complement(24488..24708)
                     /locus_tag="VW343_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24662..24922)
                     /locus_tag="VW343_001298"
     CDS             complement(24662..24922)
                     /locus_tag="VW343_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755845.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         7713 a         5449 c         4495 g         7690 t
ORIGIN      
        1 tttttttaaa aaacttaata ataaatttgc tccactgatt gctatcaata tttcaattgg
       61 taaaaaatag taattccaaa taaaatgaga aatcagcaat gatagaaaaa taacaatatc
      121 aattttcctg ttgttgacat actctgtaat tttatgatac aggaatgcaa caataactga
      181 aattaaaaca atcaacataa gattattaac ctattctcaa ttgattatca cgtcgcccat
      241 cccagccatc aataatattg gtaaaaatcc aatcagctcc agcaccaatt ccagcagatg
      301 caatacttct aataacagta tttgcaaaag catttccaat atgaattcct aatactccta
      361 aacctttaac aatcccagct accagtgcgg agcgtaatcc ataagatgca agaactagtt
      421 gaaggcctcc acctcctgct gctgctaatg ctgcatatgc tgcaccagta atggctcctg
      481 taattgccca cctgggtata tatatagccc ccccatcaag gtacatcatc tcttcttcat
      541 caatcacaac ataattattt ggtaatacaa gttccattta ttttctcctt ttttattaag
      601 catgcttagg atggtttcct aacaagtgat cgcaggcttt gtttagttac ctaaattaga
      661 cttccttcct ttatcttatt ttaaatattt aaaaccagtt actgcttgaa gcttaacagt
      721 ttacttaatt caaataacag ctaatcactg attccctgat tttatcaaat atactaataa
      781 caacaattgt gatacgatca tcattataag aacaacattt aacgaatcaa agtttttccg
      841 agataataat aaagcaacag caagcagtat tattactaaa gattgaataa ctactattac
      901 tcttaacatt ttatcctcca ctaacaactc aagctccctt acattatatc gaccagcccc
      961 atggaaagat tatagctaga ctaatcaatg atataataca tactagaata caaaatattt
     1021 ttttattcat actttttctc ctatctttat tttctaccta catcatacct cttattaagc
     1081 tcatttcaaa attacgttaa cttttttcac gaaaaggaga caattatgcg ttatgattat
     1141 ggatagcgct ttcaaatttt taaactccta tcccatcctt ttatctatat tatgagcgaa
     1201 aatataataa ctgtcaagta actaaagtga attttataaa aaaattacaa gccaaatttg
     1261 taaagtttac actaagccgc tagggcaatc gtctatcaga atgtccgttt atttgtcaat
     1321 aatccgagaa aatcttgcaa cgcttagaag tctataaaaa ctatcaacat ttatatgact
     1381 tgcgaatagc aatcctgctc aacctttcca cactctatct atacaatcaa gataaaaaca
     1441 tgtgtaagca aatctgctac actttactgg aggacgccaa gaataagaaa agctacgata
     1501 ggctggctat ctgctatgtc cgtattggga tttgtaggga tgatgcgaaa cttatccaaa
     1561 aagggttctc ccttctggag ctaaccgagg aaacttctat actgtttcat ctcaaaaaag
     1621 aagtagagat ctattaccaa gcgaaggaaa gataaaaaag ccgagggatt tcctcgactc
     1681 tttacatatt atttgttcgt tagttcttaa taccagctat tgttaagcca gaagttttta
     1741 gctctgccaa gccacatagt gtgtttacta ctatgtgtcc atgtcaccat gaatcgccga
     1801 acgatttaac gagaaagtat gacttttccg tttatcccaa ctcaattatg acattttttt
     1861 caaaagtcaa tatatctcac tttttcaacg acaagaaaga ggctgataat ctaccaacct
     1921 cttattctga acccatcact ccatcacttt ttagcttcat tcgctttctt agcgactgca
     1981 atctggtatt cgacttggtc attcccctta ccggtacaac catgagcaat tgtagtcgct
     2041 cctatctgat gcgctatttc aaccaatttt ttagaaatca gagggcggct caaggcagat
     2101 accaagggat acttttgttc ataataggca tgtgactgaa gagccactag cacataatct
     2161 gtagcaaatt cgtccttaac atcaatgaca taagattcta ctgccccaac cttaagagcc
     2221 ttatcatgga tgaaatctaa gtctttccct tcacccacat ccatacaaac tgaaacaaca
     2281 tcatagtctt tctttaacca ggtaatagca actgatgtgt ccaaaccgcc agaataggcc
     2341 aaaattactt tttctttact catgttcttt tccttctttc tatttttcaa tggatgcttt
     2401 aaaggcatcc tcaatgagtt tgtctaattc cccagactcc ttcaactttt gaatggtttt
     2461 atcgactgcc tctttcaatt ccttgctatc ttttttcatg gctaccgcgt aggaatcatc
     2521 ttgctctttt tcaaaattga ggtctgcgat tgctaaatca ggattatttt ccacaaatcc
     2581 cttggaaaca ggttcttcaa agataacggc atccacttgt cctgatttta aatctgtgat
     2641 taaattccca tttttaggca gagatacgag ggaagaattt tgtagcaaat ctttcgccat
     2701 cgtctcttga atcgaacctt tctgcgctcc aacctttttc tgcgccaagt cgtttacaga
     2761 ctgataagta gtcaagtcag attttttgac aatgagttta ttttttgcag tatagtaggg
     2821 aatggaaaag tcaaacacct tgctccgttc atctgtctta gaaacacctg atatggcaag
     2881 gtctgctttt cctgattgaa cactagccag tacattatca aaactcatgg gagatagttc
     2941 caattcgaca cctagttctg ttgcgatagc cttggctaat tcgatatctg aacccacaat
     3001 ctgatttttc ccatcaacca ctttttgata ttcaaatgga gcaaaatctg gattgagggc
     3061 tacaaccaat tttcctttgg acttgatggc ttcaattcca gccgattgat tgttactaca
     3121 agcaaatagt agggggagca tcactaaacc aaacatcgtc atcaacacct tcttcatctt
     3181 attcataaca aagccttctt tatgtttatt cttttttatt gtataaataa tactcctata
     3241 ctcatatact catcctttgt caagcctaaa ttgaaaaaat tttcaatttg aaacaaaaaa
     3301 aaacctagaa atgctacata aaatgtcatt cctaggcgta tttatgctat ttttctctgg
     3361 aaaacatgag tattcagcca gtcaaaagga gctgaatgaa ctcattttcc ctcgcccaat
     3421 tcaatgacac gatgacattg ttgggctaca taagcatcgt gggtcacaat aatgactgtt
     3481 ttcccttctc gattcatctc taagagaaac ttcaagacca aatctctatt ttcaggatcc
     3541 agcgaacctg taggttcatc ggctaaaatc agctggctgg gttttaagat ggctctagca
     3601 actgcaattc gttgttgttc gcccccagac aactcggaga ccctttgatg caaagtagct
     3661 gacaaaccta ctctctctaa aatctcttcc acctttttga gcttgtcttt cttaggcaat
     3721 ttcacatatt tcagcgccag catgagattg tactcgaccg tttcatcatc aatcagggca
     3781 aaattttgaa acagataaga gatatgttca cggattattg tttgcgactt agcagaatta
     3841 accgctagat ttgtctgacc aaaaatctca taccgtccgc tataatcacc atctatcaaa
     3901 cccaataaat ttaacaaggt cgacttccca ctaccactct taccaacaat agctaccaaa
     3961 tccccctgat caatcctgag agataagtta tccaaaatca cttttccccc aatggttttg
     4021 gtaatatttt tcaactcaat cataagatgc cccctttcaa taactctact agacttcttt
     4081 tctccatcct agaagctaag cctagcacaa atagtatatc cagacatgta aaacctgcaa
     4141 acagtagaag tggtaagaac gcatgggcaa agaaaatcaa gactagaaga gggaaactat
     4201 agcccagcaa gagcagaacg aggagaggac ggtagcgatc gaccagtttc caccccataa
     4261 acttcttggt aatgatatcc ctgcgcttca ataagaaagt tgttactagt aagaagtagg
     4321 aaatcatcat gctaaggaga ccaaacaaag caaagagtag gttaaaattc cgaacagcat
     4381 ctctataaga atccactttc tcttgttgaa tggcttgaat agatgaaaat tttaaataat
     4441 ttccatctga caatttctca actaactctg taatctcttt ttgatgttga accgtatttt
     4501 caattttaat cggattattt aagccagttg ttgacaggga ggctttctca tcccacatca
     4561 tatcagaatc attgaccaag ctaataattg gattggagag attttccttt cgcttatcac
     4621 tatatgggaa aaatgaccaa tctccttcat aataggcaat ctcgacatcc atctcctcta
     4681 tcgttcgttt ttgctgctct tcatacttca tcgaatgaaa ggcaattaac ttccccaaga
     4741 gctgattttt atcttcttca cctttcgtac ttgctggcat caaaataact tttttaatac
     4801 cggtatctgg tagcttgaat cccttgctct ttagaaaatt gcgattggca tagtaaacat
     4861 ccaccgtatc tgttaactga tattgctgaa tctgttctga ttggacaaaa ttttttacag
     4921 gaagactgct actctgcaca tagcccgcct gcgttttttc taccaaatcc tgataaaatc
     4981 gatagaaata atctgtagat ttccctgacc ctgctagctc ttcttgccac agattatcat
     5041 tgagtttgaa ggtttctaag gtcaggtaat taccttgact tacccactgt tgctgataag
     5101 caagttcttt gttttcttgt tctaaacttc tgcccacccc aatcagtaag gccgtcagta
     5161 aaatagttgt ccctattttc atcacataat tgaagataag accaaatttg aaagatgaaa
     5221 aacctttcag cagagagctg attgtcattt tttggattaa gaggtaagtc aaccaactga
     5281 taaagagata aagctgcaac agcaaaaaat gagacaacca cagcatagga aacaaatctt
     5341 ttggcttata atcaagcaag aaaaacacgc ctagattgat cacaagagcc ccacctagga
     5401 ggaggtaaag gttgcctttt acaacatcag ctaaaacagc cctatcttga aaaccaagta
     5461 atttttgtac cccaactctt ttcatctcca tcatcggttg atacactgtc actaacacaa
     5521 gaagcaaaat agccaagaca aaaacaatgg cagataaaag caaatctcga tttatgactt
     5581 ccactgcact tttgtaggtc ggctctagca aggtagcctg gtctatcttg aaaaaatcgc
     5641 tccatttctg tacaatccta tccttgtcca tctcttgtgt agaagttatc gtatagcgac
     5701 catttaaact acgagatgta tccttgatat aggtttgaaa agtcataagc tgaataggtt
     5761 tggcttttag aaaggtcgga atcgtaccaa gtttattgga aatttcttta ttactataga
     5821 ctccttcacc atctgtggta aaatcaagag aagaaatccc aaactcttgg taggggaagg
     5881 tatctttatc aaaaacacca gacttgacca cctcatcacc actgtctgtt ttgatgatgg
     5941 agactttata ctcctttgat acatcctcaa aaaatcgaag aacagacgct gcaggttcgt
     6001 taatatcttt caaatacaaa tccaaagaat ctacagtctt tcccatttct tgaatccgaa
     6061 ccacttctcg tgtatagttt acattaaaaa caaaaaaagt ggtacacaga agcaggagta
     6121 acatacagaa attactgatt tttttcataa aagtgcctcc actacagttt tccctctcca
     6181 aataggagag ggaagaattt tactcaaatc catagaaata ttccattcct gttggcggaa
     6241 tcttggtgag ggaagctcta gcccatgcct caggttttgc ataatcctta gaggttctac
     6301 ccccatgtct aacagttgct gtatgatatc gagtagaatg taagtaatca gaatatccaa
     6361 aagttcctgt ccaacctact ccatagttcc attgaccacc ctcaacccat actgctaaag
     6421 catttgtagt agcaagtata ccacatacca ttaaagatgc aacaccaatt ttaactaatt
     6481 ttctcatgat ttatttctcc tattatcgag agctgttccg taaaaacagc tatagtaatt
     6541 tatcaacaag tatcgtagtc cattggactc accttccttc tatctcattt cattttttaa
     6601 caacgaactt actcttaacc gggagaatgg tgcgcttctg tatcaggtcc ttgaacaacc
     6661 agagtagtat taagcacact ctaactatta ctcaagaatc agtccaataa acaatatgga
     6721 tagcgctttc aaatttttaa actcctatcc catcctttta tctatataat aagcaaaaat
     6781 ataataactg tcaagtaact gaagtgaatt ttataaaaaa attacaagcc aaatttgtaa
     6841 agtttacact aagccgctag ggcaatcgtc tatcagaatg tccgtttatt tgtcaataat
     6901 ccgagaaaat cttgcaacgc ttagaagtct ataaaaacta tcaacattta tatgacttgc
     6961 gaatagcaat cctgctaaac ctttccacac tctatctata caatcaagat aaaaacatgt
     7021 gtaagcaaat ctgctacact ttactggagg acgccaagaa taagaaaagc tacgataggc
     7081 ttgctatctg ctatgtccct attgggattt gtacagataa tgctaaactt atccaaaaag
     7141 gtttctcact tctggagctg accgaggaaa cttctatgct gtctcatctc aaaaaagaag
     7201 tagagaccca ttatcaacca aagaaattat aaaaaaagtc gagggagctc ctcgaccttt
     7261 tacatattat ttgttcgtta gttcttaata ccagccattg ttaagccaga agtttttagc
     7321 agcagtccat gaaccgtaac gtcctgcaac gtaggcatct gctacacgtt cttggttttc
     7381 agctgagtag tcaccgttca ggtatgaatc tgttaattgg taacgtccga tataacgtcc
     7441 atttgtagct gtatagctac cacctgattc tttttgagcg atccattctt tggcttctgc
     7501 ttcagatccg cttacagttg aaacaactgt ttcacttact actggagctg aaacagtttc
     7561 atcttgagcg gctggtgccg cataagtagc tggcgctggt gttgcaacag gcgctacagg
     7621 gccatcgata accaactctt gatcaacata aatcaaatga atgttatcaa tgtggttgtt
     7681 ttctgccaat ttttcaactg ttgtgttgtg agtttcagcg atttctgaaa gtgtatcacc
     7741 ttctttaaca gtgtaagttg atgattcttg agcagataca aatgatggag caaatactgc
     7801 aaacaaggca gctactcctg caagagttgc tttaatcttt ttagttattg atttcatatt
     7861 cgaaaattct ccttctttct atagttctat catacccttt aaatattacc gtttcttgac
     7921 acttttatgt agaaatatta caaaattatt ttttatttcc ctaaataagg tgttttttgt
     7981 cataaaagat actattttta tgctatttga ggtattagaa aggttttcat ccacaattaa
     8041 agccaagccc ctcattcttt gatataatag agataagaat ttttataagg ggaaactgat
     8101 gaaatcactt cgttttcaat ctgtctttga tatcatcgga ccagttatga ttggcccatc
     8161 tagtagccat accgctggtg ctgttcgtat tgggaagatt gtctcttcca tttttgatga
     8221 tactccgaca gaagttgaat tccaactttt taactcattt gccaagacct atcgtggtca
     8281 cggaacagac ctagcccttg ttgcaggtat tttaggaatg gatacagatg atcctgaaat
     8341 tccaaatagt ctggaaattg cccacaagcg tggtatcaag attgtctgga ccattcagaa
     8401 agacagcaat gcccctcacc ctaacaccac taaaattact gttaaaaatg cccacaagac
     8461 catcagcgtg actggtattt ctatcggtgg aggaaatatt caggtaaccg aactcaatgg
     8521 ttttgccgtc tctctcaata tgaatacacc gactatcatc atcgttcatc aagatattcc
     8581 aggtatgatt gcccttgtta cagaggcgct ttcccgctat ggtatcaata tcgcccagat
     8641 gaatgtcact cgtgaaaaag ctggtgaaaa agccattatg attatcgaag ttgacagtcg
     8701 caactgtgat gaggctatcg aagaaattcg aaaaatccct catctccaca atgtcaattt
     8761 ctttaaatag gaggaagcat gttttattct atcaaagaat tggtcgagca agcagatcta
     8821 gactttcaag gaaatgtcgc agaactcatg attacaacag agtttgaatt gaccggtcgc
     8881 gaacgtgaag aagtcttcct tctcatggaa cgcaatctgg aagttatgaa agcctctgtc
     8941 caacttggcc tcaatgaaaa taaatctcgt agtggcctga caggtggaga tgctgccaaa
     9001 ttggatcact acatcgaaaa cggaaaaact ctgtcagatt acacgattct ctctgctgcc
     9061 cgaaatgcca tcgcagtcaa tgaacacaat gccaaaatgg gcttggtctg tgccactcca
     9121 accgctggaa gtgctggctg tctgccatcc gttctcactg ctgctattga aaaattagac
     9181 ctcagccacg agcaacaatt ggatttcctc tttgctgctg gtgcctttgg actagtcatc
     9241 gcaaacaatg cctccatctc aggtgctgag ggtgggtgtc aagctgaagt tggttcagcc
     9301 tctgctatga gtgctgccgc cttgactctg gctgcaggtg gaacacctta tcaggccagt
     9361 caagctattg cctttgtcat taaaaatatg ctaggcctca tctgtgaccc tgttgcaggt
     9421 ttggtcgaag ttccctgtgt caaacgtaat gccatgggag ctagctttgc tttcatcgca
     9481 gcagacatgg ccttggcagg tatcgaatct aaaatccctg tggatgaagt gatcgatgcc
     9541 atgtaccaag taggagcaag catgccaact gcctttcgtg aaacagctga aggtggactc
     9601 gctaccaccc ctactggtcg tcgcctccaa aaagaaattt tcggagaata agcttatcaa
     9661 ataggagaaa ccatgacctc tatcacagcg atttttttcg atttggatgg aaccctcgtt
     9721 gatagttcta tcgggattca caatgccttt acctatacct ttaaagagct gggggtgcct
     9781 agccctgatg ccaaaactat tcgtggtttt atgggaccac ctctcgaaag tagttttgcg
     9841 acctgcctgt ccaaagacca aatttctgaa gccgtgcaga tatatcgttc ttactataag
     9901 gcaaaaggca tctatgaagc tcaactcttt cctcagatta tagacttgct tgaggagtta
     9961 tcgagcagtt atccactcta catcaccacg acaaaggata catctactgc tcaagacatg
    10021 gcaaaaaact tggaaatcca tcatttcttt gatggcattt atggttctag ccctgaagca
    10081 ccccataagg cagatgtcat tcaccaagcc ttgcagacac atcaactagc accagaacaa
    10141 gccatcatca tcggagatac caagtttgat atgctgggag ctcgagaaac aggtattcag
    10201 aaattggcca tcacttgggg atttggagag caagcagatc tactaaacta tcaacctgat
    10261 tatatcgctc acaaaccctt agaagttttg gcgtattttc aatagcatag actgggtaac
    10321 aattccattt cagaagaaaa tgaagtaata ctcaatgaaa atcaaagagc aaactaggaa
    10381 actagctgcg ggctgctcaa aacactgttt tgaggttgca gatagaactg acgaagtcag
    10441 atcaaaacac tgttttaagg ttgtggatgg aactgacgaa gtcagatcaa aacactgttt
    10501 tgaggttgca gatagaactg acgaagtcag atcaaaacac tgttttgagg ttgtggatag
    10561 aactgacgaa gtcagtaacc atatacggca aggcgacgtt gacgtggttt gaagagattt
    10621 tcgaagagta taagtctcta cataataaaa cgcatattac caaggttgtg taactggata
    10681 ctatgcgttt ttctttttta ttcaatgtgg gttgtttgat tagcaaaggc gataatagct
    10741 tgcttcaact catctccact gagagtgcgg aactctccaa tcttttgatt gatggattct
    10801 aaaggcatga cattaacctt accaacgaaa atcttatcca taacttgatt atcaacgagt
    10861 tcggttgata ccaagagttc ttcgtagagt ttttcacctg ggcggattcc aacttcaacg
    10921 attggaattt cactttcagt gtggccactt agaagcacca tcttcttggc caagtcataa
    10981 atcttgactg gtttgcccat atcaaggata aagacttccc catctttggc ataagcacca
    11041 gcatggataa ccagacggct agcttctgga atggtcataa agtaacgggt catgcggaag
    11101 tctgtcaccg ttacaggccc accttcagca atctgacgtt caaagactgg aatgacacta
    11161 ccacggctac caagaacatt cccaaaacga actgcacagt aggttgattg gctacgttgg
    11221 ttaaagccag tgacaatcaa ctccgccacg cgcttggttg ctcccataac atttggtgga
    11281 ttgactgcct tatctgtcga aatcataacc atcttagaca ctttagcttc atcaacagcc
    11341 ttagcaacat tgtaagttcc acggatattg tttttgaagg cttcttttgg attgcgctcc
    11401 atcataggaa catgcttgtg ggcagccgca tgataaacaa tagcaggttt gtactgctca
    11461 aagacttgca acaaacgatc atagtcttga atgtccgcaa tcacaggtac ataatcaatc
    11521 ccttggaact tacgaatcaa ttcatgataa acaaggtaga ttgagttttc cccatgaccg
    11581 agcaagacaa tgcgttcagg attgaagcga ctaacttgac gacagatttc agaaccgatt
    11641 gaacctccag ctcctgtgac taagatggtc ttacctgtca gttctgcacc cagacgcgat
    11701 tcgtcaagac ggatttcctg acgacccaaa aggtccgtaa tatcaatttt ttggaagcca
    11761 gtacctgctt ggtgaaggcc ctgaacaaca gtttcaacct taggcatctt gtaacatttg
    11821 acacccagct tattacacat ctgcaagata cgctcatatt ctgacggatc cagcgacgga
    11881 atcgcaacga tgacacgctc gatttgatgg cgtttggcta attcaggcag attgtcataa
    11941 gagcccaaaa caggaatacc accaagtttt tgaccctttt tcttagaatc cttatccaaa
    12001 ataccgacca gttctaattc actggttgga tgttggtaac tatccataaa gagagcccca
    12061 ccatcaccgg caccaatcaa gaaggttcga cggtgttctc catcaccact accttttttg
    12121 cgtctggagt agattaactg ccaagtaatc cgtggcaata aaatcaagaa ggtactcaac
    12181 aagataaaga gaatgatgaa acggatggag aagagtggca agaaggcata acagatacta
    12241 tatgacaaga cactgctagc agtcacacca aaaaagattt tcatgaaatc cgtaatcttg
    12301 ctgtaacgac taatgctcgc gttcaacccc caaaaaccaa tcatcaattg atagaacagg
    12361 aaggccaaac tcgtatagat aatgtagtca acaggtgctg gattaatcag cccataaaat
    12421 aaaatataag atacaatgat ggaaaccacc atactgaaaa tatcaaatat tccccagaaa
    12481 acctgctttt gttgtttatt taaaatttcc accagatcaa tcacataatc tgttagtttt
    12541 ttattcatag aaatctactc ctccttaaaa gagttttact atattgttat tatagcatat
    12601 tttgatttat ttggcttatt ttatccaaat aaattattgt tctcttgtac tccttcttcc
    12661 atttaaatcc tctaattaaa cctacaatcg tcccaaagcc ataagcaaag tgaatggaaa
    12721 ataaaagaaa gggcatcaca attagaaatc catttttatg ttttaataaa gtcagcaaag
    12781 tgagtaatga caaaagtaga aaataggcac ctaatagtaa agttatgaat acgaatgtga
    12841 tcggtaacaa tgctagacta aacacaagac tcaaaacaaa taaacaagga acatagtgaa
    12901 ataatgataa acacttaaac tgaacatgac ttgtcaagcc aatccacaaa ccatttgaat
    12961 acttttgatg cagcattttc ttgaatgttg gtcgaatata ctgataagat agaatacttg
    13021 ggctatagcg gattttataa ccatgttctc gaattctata atgaatatca ttatcttcag
    13081 ttcggccaag ttgctcattt actaaaccaa ccttctggaa aacctctcgt ttatacatcc
    13141 catgaaaaat agaagaaaca tatctatcct cggaactatt tcgataattg gcaatgctac
    13201 tgccaaacat attttcctca acaagatgca aggtctctgc ccattttcct tttccttcga
    13261 caatcgtcgg tctaggcccc ccacagacaa attcaccttg ttgaataata gccacattgt
    13321 tcattacaaa actctcagta acttttgaat gagcatcaat ttttaaaata aggtccccta
    13381 cagaatgttt aactcccagg ttaaaaccac tagcttgatt tttcttagga ttgttataca
    13441 atctaattga gttaaactct gtatcttcct ttataaattg ctgaatgata gctgtggtcc
    13501 catctgtgga catagcattt ataaatagaa tttcaatatc ctctttagga taggtttgat
    13561 tttttaagtc ttcaattaga ccaggcagat atttttcttc attgtatgca ctgattacaa
    13621 tggatacgag aagactttca acattttgag tcactactac tctcctgaat ttttccttcc
    13681 accattctat cataattttc aagactttcc catttttatc ttgaaagcca gaaaccttac
    13741 ttgacattat agtgaaaaag ccttaaaata agctgttatt aaaatcagct agaagaggta
    13801 ttatatgaaa aagcaatcac tcttttttgt tccaggtatt atcctgattg gtgtttcctt
    13861 gcgaactcct tttactgttt tacccattat tttgggaaat atttcgcaag gtctggaggt
    13921 agaagttagt tcgcttggtg tcttgaccag cctgcctctc cttatgttta ccctcttttc
    13981 accattttct acccaactgg ctcagaaaat cggcttggag catctcttca cctacagcct
    14041 cttcttcttg accatcggct cacttattcg actaatcaat ctgcccctgc tctatctagg
    14101 aaccttgatg gttggggcaa gtgtcgcagt catcaatgtc ctgcttccta gtcttatcca
    14161 agccaatcaa ccaaagaaaa ttggttttct gaccacctta tatgtaacgt ctatggggat
    14221 tgcaacggct ctggcttcct atctagctgt gcccattaca caagccagtt cttggaaagg
    14281 acttatcctt ctcctcacgt tactctgtct agcaactttt ttggtctggc tcccaaatca
    14341 ccgctataat catagactag ctccacaaac caaacaaaaa agtcaaataa aggtcatgcg
    14401 taataaacag gtttgggcaa ttattatctt ttcaggtttt caatccttga tcttttacac
    14461 cgtcatgacc tggttaccta ccatgtctat ccatgcaggt ctatccagtc acgaagctgg
    14521 cttgctgact tctatcttat ctctgattag cattcctttt tcaatgacca tcccaagcct
    14581 gacaaccagt ttatctactc gcaaccgtca gctcatgctc actctggttt cactagctgg
    14641 tgtggtcggc atttccatgc tctttttccc aatcaataat ttcatttact ggcttgccat
    14701 ccatctcctc atcggaaccg caaccagtgc cctcttccct tatctcatgg tcaacttttc
    14761 actcaagaca agcgcccctg aaaagacagc ccaattgtcc ggcctatctc aaacaggagg
    14821 ctatatccta gcagcctttg ggccaaccct ctttggttac agttttgacc tgttccactc
    14881 ttgggtacca tctgtagctg ccctcttgct catcgatatc ctgatgactg tggccctctt
    14941 tacagtggac agagcggata agatccttta aacttctagt tttagctaga agttttttat
    15001 atataaaaat tttacacatt tctcttgaca gggctttctt ttagatgtac aatgtatgtg
    15061 tagaaaaatt atatataaaa atcttacaca ttagaaaagg aggtttccca tgtactttcc
    15121 aacatcctct gccttgattg aatttctcat cttggctgta ctggagcagg gtgattctta
    15181 tggttatgag attagccaaa ccattaagct gatcgctaat atcaaagaat ccacactcta
    15241 tcccattctc aaaaaattgg aaggcaatag ctttctgaca acctactcta gagagttcca
    15301 aggtcgcatg cgcaaatact actccttgac aaacggtggt atagagcagc tcttgaccct
    15361 aaaagatgaa tgggcactct atacagacac catcaatggc atcatagaag ggagtatccg
    15421 ccatgacaag aactgaatac ctgactcagc tagaactcta tctcaagaaa ctacctgaag
    15481 ctgaccgtat cgaagccatg gactatttca gagagctctt tgacgatgct ggagtcgaag
    15541 gagaagaaga actcatcgct agtttgggaa ctcccaaaga agcggcccac gaagttctat
    15601 ccaatcttct cgataaaaaa atcaatgaag cacccgctca aaaaaataac cgacaaattt
    15661 tacatatcgc cttgttagcc ctccttgcag cacctatcgg cattcctctg ggaatcgcca
    15721 tcctcgtgac cctgttcgca attcttgtag ccgctttgac tgtcattctg gctttctttg
    15781 cagtttccat actgggtatc atcggcggat tcctattttt agttgaaagt ttcactgtcc
    15841 tcgcccaagc caaatcagcc tttatcttga tttttggttc tggtttactg gctatcggtg
    15901 cttcttcgct agttttactt ggcatttcct atgcaactcg cttcttcggt ctactcattg
    15961 ttcgtctggt acaatttgtt cttaaaaaag gaaagagagg taatcagcat gcgtaaatgg
    16021 acaaaaggat ttctcatctt tggtgtggtg actaccgtta tcggctttat cctgcttttt
    16081 gtaggtatcc aatctgacgg gattaagagc ctactttcca tgtccaaaga acctgtctat
    16141 gatagccgta cggaaaagct aacctttggc aaggaagtcg aaaacctaga aattactctc
    16201 caccaacaca cgctcaccat cacagactct ttcgatgatc aaatccacat ttcttaccat
    16261 ccatctcttt ctgctcacca tgatcttatc accaatcaga acgatagaac tctgagtctc
    16321 actgataaga aactgtctga aactccgttt ctctcttctg gaattggtgg gattcttcat
    16381 atcgcaagta gctactctag tcgttttgaa gaagttattc tccgactacc aaaagggaga
    16441 actctaaaag ggatcaacat ctcagccaat cgcggacaaa ccaccatcat aaatgctagc
    16501 cttgaaaatg cgaccctcaa tacaaacagc tatatcctcc gaattgaagg aagtcgtatc
    16561 aaaaacagta aactcacaac gcccaatatc gttaatatct ttgatacagt tcttacagat
    16621 agtcagctag agtcaacaga gaatcacttc cacgctgaaa atatccaagt ccatggcaag
    16681 gttgaactga ctgccaaaga ttatctcaga atcatcctag accagaaaga aagccaacga
    16741 attaactggg acatctcaag caactatggt tctatcttcc aattcacaag agaaaagcct
    16801 gaatcaagag gtacggaatt aagcaaccct tacaaaactg aaaaaaccga tgtcaaggat
    16861 caactcattg cgagatctga tgataatatt gatctaatat ccacaccaag cagacgttga
    16921 caaaaacgct tacatttgct actatggaaa catatttatc taactaaaaa ggaggttcta
    16981 ccatgaaaca agaatggttt gaaagtaatg attttgtaaa aacaacaagc aagaacaagc
    17041 ctgaagagca agctcaagag gttgcagaca aggctgaaga aacgatagcc gatctcgata
    17101 caccaattga aaaaaatact cagttagagg aggaagtccc tcaagctgaa gtcgaattgg
    17161 aaagccagca agaagagaaa attgaagctc ctgaagacag tgaagcgaga acagaaatag
    17221 aagaaaagaa ggcatctaat tctactgaag aagagccaga cctttctaaa gaaacagaaa
    17281 aagtcactat agctgaagag agccaagaag ctcttcctca gcaaaaagca accacgaaag
    17341 agccacttct tatcagtaaa tctttagaaa gtccttatat ccccgaccaa gctccaaaat
    17401 ctagggataa atggaaagag caagtgcttg atttttggtc ttggctagtg gaagcgatca
    17461 aatctcctac aagtaagttg gaaacaagta tcacacacag ttacacagcc tttctcttgc
    17521 tcattctgtt ttctgcatct tcctttttct ttagtatcta tcacatcaaa catgcttact
    17581 atggacatat agcaagcatt aacagtcgct tccctgagca gctagctcct ttaactcttt
    17641 tttctatcat ctctatccta gtagcgacaa cactcttctt cttttcattc ctcttgggta
    17701 gtttcgttgt gagacgattt atccaccagg aaaaggactg gacgctagac aaggttctcc
    17761 aacaatatag tcaactcttg gcaattccaa tcttcctcac tgctattgct agtttctttg
    17821 ctttctttga tagcctacga tttacagccc tcttgtgtgt gattagcatt ggaatcattc
    17881 tgcttgccag tctccatatc attacaagac caagtcaagc aagtgaaacc gactccttct
    17941 atcaattatt cttgtctgtc cttgtgaacg gagttattat cctcctcttc tttgtagctg
    18001 aagtggcact gattggagat tatcttcgta tcttggcctt tctttaaact tcatactctt
    18061 caaaaatcaa attcaaacca cgtcagcttc gccttaccgt actcaagtac agcctgcggc
    18121 tagcttccta gtttgctctt tgattttcat tgagtagcaa tcctgtcatc cttggcagga
    18181 ttttttctat gtcaaaaaag caagtcgatt gacctgcttc tgctttactt ttcttgtgct
    18241 aatgttttaa aatctttgtc taatatgggt tgcacttcta tttgattggc gtctagttgg
    18301 tggtaaaaag atgggggtaa tacctctaag aattttgcat agtccaagcg agcaatggct
    18361 tcatagtctt ctggtttaga gccatctcct gtgatttgaa ccaagtcaat ctcccactgg
    18421 acttccttat ccaccaattg ctcaatatag gctgcaggaa caaacggttc tctcggtaaa
    18481 actgtcttga ctccttgagc tagatggtaa acgccatgca cttttccttc tccatcctga
    18541 tagaaggaaa ctctctcaaa gtaggcatct gtctcttgaa ccgcacgcac acgcgcttca
    18601 aactgagcca agccatctgc ctctaaaaag cttttcaagt tctcacgact aaagaaaata
    18661 ggattaaaaa ttccttggca aatcgtaaaa cgagctacag ggtctgctag ataggcttga
    18721 atacttgctt ggaaataagc ttcaaaatca aaaccatcta gcccttcaat atcagtccct
    18781 gtataagcaa gcaaagcatc taaaaatgac ttgataatct gccagtctgt ctttgttagt
    18841 aggtcaggaa gatcgacacg gtaagccttt tgaccatcag cataactgac cttaaagaga
    18901 aattgtgatt gccccactat cgcacactcg atatattcga gacgattaag aggctgacgg
    18961 agatagacta catcatagct atgtgactcc aaaccgtcta gcaaggccaa gatcgacttg
    19021 gcattcaaaa tctcttgttc tcctaaaatg ctctgtttat ttggaataaa aaatgttttc
    19081 gccataactg gactcctttg aaatcgttta catacagtat accttatttt tctgaaagat
    19141 acaaaaacaa actttatact cttcgaaaat ctcttcaaac cgcgtcagct tcgccttgcc
    19201 gtagatatat gtaactgact tcgtcagtct tatctacaac ctcaaagcag tgctttgagc
    19261 agcctgcagc tagcttccta gtttgctctt tgattttcat tgagtattag atttttgagt
    19321 acaaagcata gaaaaacagc ccctaaggac tgtttgatct tatacagtag ctgcctgatc
    19381 caagctttca ccgatagcgg ctaggcgctc aatcacttca gcttgtgtca attcattttt
    19441 tgaaacatag cggttacgtg ggtgaacacg gcactcgtgt gagcatccac gaaggtactt
    19501 gtcttcattt tcttctgatg tcaagatacg acggttacag aagggatttc cacagttgac
    19561 ataacgttca catggtgttc catcaaacca gtctttccct acgatagttg ggttgacatg
    19621 gttgacatca acggcaatac gctcgtcaaa gacgtacatt ttcccatccc aaagctcacc
    19681 ttgaacttct gggtctttac cgtaagttgc gattcctccg tgcaattggc cgacatcttt
    19741 gtagccttca cggaccatcc agcctgagaa tttctcacag cgaacgccac ctgtacagta
    19801 aaccacgaca cgcttgtcca tgaatttttc cttgttatca cggacccatt gtggtaactc
    19861 acggaagttg cgaatatctg ggcgaatagc cccacggaaa tgtcctaggt cgtactcata
    19921 atcgttacgt gtgtcaagga caacggtatc tttatcaaga agcgcttctt tgaactcttt
    19981 tggagacaag taagcacctg ttgtttcaag tgggttgatg tcattgtcaa agtcgttgtc
    20041 ttccaaacca aggtggacaa tttctttctt gtagcgaaca aacatcttct tgaaggcttg
    20101 ttctctttct tcgtcaatct tgaaccagag atcttccata cctgggaggc tgtgaacgta
    20161 gtccatgtat ttttgagttg tttcatagtc acctgaaact gttccgttaa ttccctcgtc
    20221 agcgactagg atacggcctt taaggccgat tgatttacag aaagccaagt ggtctgcagc
    20281 aaattgctct gcattttcaa ttggagtata aaggtagtaa agtaagacac gaatatcttt
    20341 tgccataaga tttgttctct tttctattct taaattttct aaatttttta tttatccata
    20401 ctagtatacc tgcttgagaa aacgaatgca atttatttga ctggaaattg tagagggttc
    20461 tcgaaatttc ctgaatggtt aaaataagga caagagaaaa catggatatc tatatccttg
    20521 tgccaaaaaa accactgccc tccccagacc aacctgagga aagcagtgat tcttatttta
    20581 ggagttagga atgaatacac gaaatcaatt tagctgatta ttttttgttt ttcaagaatt
    20641 catcgtattg tttttgcatt tcgttcaata ctttttcgta ggcaccttca gatttcaatt
    20701 tttccatcaa ttctggaata gctttatctg ggtctacagt accagtgttg atagctgtat
    20761 caaattgttg cattgtgtta gcaatagctg agatttcaga tttcacattg tcagtattga
    20821 agataaatcc aagcgctgga gattctttag cttctgccaa ttctttctta gaattttcga
    20881 tttgttggtc tgtaacgttt tcgttgatgt aaaggatcca gttgttacca gtgttccatc
    20941 cacccatgtg agtgtttcct ttgtagccat caagaacgcg aacacggttt tctttacctt
    21001 caattttttc ccagttcttg ccttctggac cgtaaacaag accgttcaag agttctggat
    21061 tcgtattcaa gaggttcaag atttccattg atttttcttt gttcttagag ttgtttgaga
    21121 tgacaaagtt agcaacttgt gttgtttggt ttttcttgat gaagttagta attggtttga
    21181 tttggatatc tttgttggca acacgtgaaa gcaagctgtt accgtagtca gctggtccta
    21241 ctgtttcttc acgaacgaac caagtatctt gttgaaggtc aaaggaagta tcgcttgttg
    21301 cgacatcttt tggaatgtag ccagcttcat agaatttgtg aagagtcttc aagtgttctt
    21361 tgaaacgagg cacttcgtaa cggtttacaa ctttagtagt atcgccttca aggtcaataa
    21421 cgaatggaag cccgtttgct actgggtagt caaaattatc agatgggatg aaaactttac
    21481 caatagcaaa tggtactacg tctggagctt tttctttgat ttgtttcaag actggctcaa
    21541 gagtttcgta agaagtaaca cctgaaatat cgataccata tttggcaagg agagttccgt
    21601 tgaaggcaaa gttttgagat gatgctacgt tggctgcaac tggtacagca taaatcttac
    21661 catttacagt attacccttg atataagctg gatcaagtgc cttgtaaagg tctttacctt
    21721 cttttttgta caattctgtc aagtcagcat aagcaccttt ttgagcattt acaatatagt
    21781 tatctgcaaa ggcgatatca tagttttcac cagatgatgt gataactgac attttcttac
    21841 catagtcacc ccagccaagg tattggatat ccaatttggc accaactttt tcttcgatga
    21901 ttttgttggc atttgctaac aattcatcca agttatctgg tttgtcaccg atttggtaca
    21961 ttttgataac aggtttgtca cctgaatcag cagctttttt actgttacct gtcaagttgc
    22021 cacaagcagc aagacctgca gccaaagcga ctacactagc agatgcaaaa gcatattttt
    22081 tccagttttt catgataaaa actccttttt ttatttttaa acttataaac aatgtaatga
    22141 tcttatactc aatgaaaatc aaagagcaaa ctagaaaact agccgcaggc tgtacttgag
    22201 tacggcaagg cgacgttgac gcggtttgaa tttgattttc gaagagtatt aacttcacac
    22261 aagggaagtt gggaactgag aaatgttatt tctcaataag cactattctt tcacaccacc
    22321 gatagtcaaa ccttttacaa agtagcgttg gaaaaatgga tacaaaatcg cgattggaag
    22381 ggttgcaacc acaaccatgg ccatacgacc tgtttctttc ggtagagcaa ctcccagttg
    22441 accagtcaag ccgaccgctt tggcaatgta gtccatattt tgttggattt gcatgagcaa
    22501 atattgcaat ggatacaagt tgtcactctt gatgtaaaga agggcgttga accagtcatt
    22561 ccagaaacca agagctgtta agagcgtgat ggttgcgata cctggtagtg acaatggcaa
    22621 acagatttgg aagaaaatcc gggcttcact ggcaccatcg atacgagccg attctagaat
    22681 ggcttctgga atggtcttct tgaagaagga acgcatcaag atgatgttaa atggtgagag
    22741 aagcattgga acaatcaagg cccaaacagt atccccaagc tgaagcacac gagtcaccat
    22801 gatataacct ggtaccaaac cagcgttgaa caacatactg agcagaacga agatggtaaa
    22861 gaatctgcga tacttaaagg ttgtccgtga aatagcgtag gcataggttg ttgtgataaa
    22921 gacatttgtc aatgtcccaa ctacggttac aaagacagag atgaagaggg cttgtaggat
    22981 tttatcctta aactgtgcca aaaactcaaa accgtctaag ccaaattggg atgggaagaa
    23041 gctatagccg tattggagga ggcttttctc gtctgtcact gaaataatga taacgaatac
    23101 aaaaggtagg atacaagaga gggcaatcaa acccgaaatg atactgaaga agatatctgc
    23161 tttcttactg aaggagtgaa tgccgacatt atcaattttt tcttttttaa ttttcttttt
    23221 tgccatattc tcctcctttc tagaacaaag ctgagtttgg atcgactcgt cttgcaagca
    23281 agtttgatag gataaccaga atcaaaccaa caacggattg gtaaagaccg gctgctgcag
    23341 ccataccgat atctgctgtc tgagtcaaac cattaaagac atatacgtcc aaaacgttgg
    23401 ttacattgta aagctgacca gcattgtgtg ggatttgata gaagagacca aagtctgcgc
    23461 ggaagatatt tccgactgca aggatggtca atacagttac aagcggagtc aactgaggaa
    23521 tggttacgtt gcgaatacgt tgccacttgc tagctccgtc cactgtcgct gcttcgtagt
    23581 aggttggatc aattcccatg atcgtcgcat agtacatgac actgctatat ccaaagcctt
    23641 tccaaatacc taggaaaagt aggagatagg gccagatgcc caggtcagcg taaaaattga
    23701 cttctttgag accaagactt tccaatagat gattgaacac ccctttatca atatttagaa
    23761 aggcatctgt aaagaaactg atgataaccc aagacaagaa gtaagggaac aacatagaag
    23821 tttggaaaat cttcaccatt ctcttagaac ggagctcgct gaggataatg gcaatcccta
    23881 cagatacaac taaacctaga aagataaagc caagattgta gaggacagta tttcgtgtga
    23941 taataaaggc gtctcttgaa ctaaataaga atctgaaatt atcgagtccg acccatttac
    24001 tatttatgat actatctatg aaaccattac tggtcatgtg gtagtctttg aaggcaacca
    24061 cgttcccaaa tactggaatg taaaagaata gaatcaacca gagtgtccct ggcaaaacca
    24121 tcaagagaaa gatccagttg tctctcaatg tttttgaaaa ctttttcata atttcctccc
    24181 tttttatttt gatatccatc taaaaattct tttttagact tttgataacg attacattat
    24241 tagtatactc ctatttgcag gttaggttaa actcctaatt atagaaaaaa ctccacaaat
    24301 tatgtagcag atttaaaact ttatcaccac tatcaaacaa atgtcctaaa tcaattgttt
    24361 attttatctc tattagccca gtgatggcgt cactctgtta taagcatcca acaacggggt
    24421 atactgaaaa atctccagac tagggaactc agagatagtt cctaatctgg agatttttaa
    24481 tatgttatta ggcgtttgct ttcaacttag caataacctc tttaagatta tcaatcacct
    24541 ctgcttcagt atgctcagag ctttttcatc tgccaagaac aaaactgctt tttgaagttc
    24601 tttttgagag ttttcaagga catccttatc tactgtttca aggtttgagt ctttaagaag
    24661 tttacttaat tccttggcta atttcttgag tttgatttgc agactcatct tctcctgctg
    24721 tttctttgcc cgctgtttgt cctccatcct tagttgctga ctggctttcc ttaatggact
    24781 ctagggaagc aatggcatct ttgactgttt gcaaaatatc acgtaaacct tgctctgtca
    24841 aactatcatc tgcaaaagct ttcttagcct ctgccaaaac cagacgtgct gaatctgtgg
    24901 taggattcga tacacctgtc aatgatctca aaagattttc taaggtttga gtctgcttac
    24961 taatactaga ctaaaatcaa aaagcattat ataacagtga tatgaaatca actaaagaag
    25021 aaatccaaac tatcaaaaca cttttaaaag actctcgtac agctaaatat cataaacgcc
    25081 ttcaaatcgt tctatagtaa aatgaaacaa gaacaggaca aatcgatcag gacagtcaaa
    25141 tcgatttcta acaatgtttt agaagtagat gtgtactatt ctagtttcaa tccattatat
    25201 ttcgtctgat gggcaaatct taataaagag attatagagc ttttatagtg atttgaaata
    25261 agatgtgaac aactctatca ggaaagtcaa attaatttat agaaatattt tagcagccaa
    25321 ggtgtactgt tataaattca atacact
//
LOCUS       DAVKMU010000027        24574 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKMU010000027 DAVKMU010000000
VERSION     DAVKMU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.027"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(68..1843)
                     /gene="spxB"
                     /locus_tag="VW343_001299"
     CDS             complement(68..1843)
                     /gene="spxB"
                     /locus_tag="VW343_001299"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9755846.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2050..4293)
                     /locus_tag="VW343_001300"
     CDS             complement(2050..4293)
                     /locus_tag="VW343_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9755847.1"
                     /translation="MTEIVKASLENGIQKIRIRSEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4303..4674)
                     /locus_tag="VW343_001301"
     CDS             complement(4303..4674)
                     /locus_tag="VW343_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9755848.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(4685..5080)
                     /locus_tag="VW343_001302"
     CDS             complement(4685..5080)
                     /locus_tag="VW343_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9755849.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5264..5362
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5446..6237
                     /gene="thiD"
                     /locus_tag="VW343_001303"
     CDS             5446..6237
                     /gene="thiD"
                     /locus_tag="VW343_001303"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9755850.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQAKDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6446..7078)
                     /locus_tag="VW343_001304"
     CDS             complement(6446..7078)
                     /locus_tag="VW343_001304"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9755851.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7071..7874)
                     /locus_tag="VW343_001305"
     CDS             complement(7071..7874)
                     /locus_tag="VW343_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9755852.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLNYRPTVIKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEAYE"
     gene            complement(7874..8398)
                     /gene="thiW"
                     /locus_tag="VW343_001306"
     CDS             complement(7874..8398)
                     /gene="thiW"
                     /locus_tag="VW343_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9755853.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(8403..9095)
                     /gene="tenA"
                     /locus_tag="VW343_001307"
     CDS             complement(8403..9095)
                     /gene="tenA"
                     /locus_tag="VW343_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9755854.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9106..9756)
                     /locus_tag="VW343_001308"
     CDS             complement(9106..9756)
                     /locus_tag="VW343_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9755855.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(9758..11143)
                     /locus_tag="VW343_001309"
     CDS             complement(9758..11143)
                     /locus_tag="VW343_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755856.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11144..11704)
                     /locus_tag="VW343_001310"
     CDS             complement(11144..11704)
                     /locus_tag="VW343_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9755857.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(11966..12054)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12263..12892)
                     /locus_tag="VW343_001311"
     CDS             complement(12263..12892)
                     /locus_tag="VW343_001311"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9755858.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGAITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(12894..13676)
                     /gene="thiM"
                     /locus_tag="VW343_001312"
     CDS             complement(12894..13676)
                     /gene="thiM"
                     /locus_tag="VW343_001312"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9755859.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            13767..13853
                     /locus_tag="VW343_001313"
                     /pseudo
     CDS             13767..13853
                     /locus_tag="VW343_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(14003..14671)
                     /locus_tag="VW343_001314"
     CDS             complement(14003..14671)
                     /locus_tag="VW343_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9755860.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYNV"
     regulatory      complement(14886..14974)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15228..16364)
                     /gene="lctO"
                     /locus_tag="VW343_001315"
     CDS             complement(15228..16364)
                     /gene="lctO"
                     /locus_tag="VW343_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9755861.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(16989..18479)
                     /gene="lysS"
                     /locus_tag="VW343_001316"
     CDS             complement(16989..18479)
                     /gene="lysS"
                     /locus_tag="VW343_001316"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9755862.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINKPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(18607..18753)
                     /locus_tag="VW343_001317"
     CDS             complement(18607..18753)
                     /locus_tag="VW343_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9755863.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            18786..19703
                     /locus_tag="VW343_001318"
     CDS             18786..19703
                     /locus_tag="VW343_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275026.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU9755864.1"
                     /translation="MYIAVWGSLGSFLLGLIVSIIRHYRIPVLAQVATAYIELSRNTP
                     LLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSAMQDSGIQVLFQGNNLLRILQGL
                     GVTIGISILSVLLSMMFGTVMGIIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFG
                     LARNFNINISGETSVIIVFTLWGTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYY
                     HIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTTSFW
                     IYGTILILYFAVCYPISKLSTHLEKHWRN"
     gene            19704..20462
                     /locus_tag="VW343_001319"
                     /pseudo
     CDS             19704..20462
                     /locus_tag="VW343_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            20474..21334
                     /locus_tag="VW343_001320"
     CDS             20474..21334
                     /locus_tag="VW343_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9755865.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGH"
     gene            complement(21414..22061)
                     /locus_tag="VW343_001321"
     CDS             complement(21414..22061)
                     /locus_tag="VW343_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755866.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKPGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(22058..22756)
                     /locus_tag="VW343_001322"
     CDS             complement(22058..22756)
                     /locus_tag="VW343_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755867.1"
                     /translation="MGKLSSYMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(22766..23518)
                     /locus_tag="VW343_001323"
     CDS             complement(22766..23518)
                     /locus_tag="VW343_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755868.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(23518..24282)
                     /locus_tag="VW343_001324"
     CDS             complement(23518..24282)
                     /locus_tag="VW343_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755869.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(24378..24560)
                     /locus_tag="VW343_001325"
                     /pseudo
     CDS             complement(24378..24560)
                     /locus_tag="VW343_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7608 a         5327 c         4423 g         7216 t
ORIGIN      
        1 agttctgctc tatgaaaatc aaagaataaa gtctacaagt ttcatatttg attttcggcg
       61 agaggaatta tttaattgcg cgtgattgca atccttcttc ttccaagaag agacggaatg
      121 gtacgagttc ttctgcttcg tatttttcct tgaaggcttt gatagcttct tctgagtgaa
      181 gttttggatc caattcaagt acttctactg gaagtggacg gtgttgagtg atgcgagcat
      241 cgatgacaac agttttacct tctttgttca atttaacagc ttctgcaaca actgcatcga
      301 tgtcttcgat acggtcaact gtgaatccaa cagctccttg agcttccgca attttagcgt
      361 agtcagcgtt tgtgaagtct acaccaaaca agtgtttgtt tgtatcttcg tatttgttct
      421 tgatgaagcc gtactcagca tttgagaaga caaggttgat aactggaagg tcgtattgaa
      481 cgtttgtgat aacgtctggg tagcacatgt tgaatgctcc gtcacccatg atgttccata
      541 cttggcgatc tggattgtct ttcttagcag cgataccacc aggaagggca atacccattg
      601 tcgcaaagag tggagatgta cgccacatgt tcttaggtgt catgtgaagg tgacgagtag
      661 atgtttgagt agtgttacct acgtcgattg agtagatagc gtcttgatca gcatgtttgt
      721 tgattgcatt gtaaacttga tacaattgca attcaccctc agttttacct tcgagtttgt
      781 tcatgtaatc acgccagttt tggttgttct taacgtttgc acgccaccat ggagttgatt
      841 caactgggtt tactttgtca aggatagctt tagctgcttg accagcatca ccaaggattg
      901 aagcgtcaag agcatgacgt ttaccaagtt tgtaagggtc gatatcgact tggataaatt
      961 tttcagtgtt cttgaatgct tcgtaaactt cagcgaatgg gaagtttgaa ccaaggaaaa
     1021 gaactgtgtc tgcttcaaag accacttcgt tggctggttt ccaaccaaca cggtaagcag
     1081 aacctgtcaa accttcatag ttccattcga aagcttcaaa gtttttacca gttgtgatga
     1141 ttggtgcttt gattttacgt gacaattcag taatcacttc accagcttta acaccaccaa
     1201 atccagcata gataactggg cgttcagcat tgttcaagat ttcaacagct ttgtcgattt
     1261 caacttcgtt caaagcagga gcgatgaatg agcgttcgta tgaacctgaa ccgtagtatg
     1321 agttttcatc gatttcttgg aaaccgaagt ttactggaat ttcaacaaca gctggacctt
     1381 ttttagaaac tgcagcacgg caggcttcgt caattacttt tggcaattgc tcagcgtaag
     1441 ctacacgttt gttgtaaaca gcgataccgt tgtacattgg gttttgatta agctcttgga
     1501 aagcatccat gttcaattcg ttaactggac gtgatccaag gatcgctagg aatggagtgt
     1561 tatccatagc tgcatcgtaa acaccgttaa tcaagtgagt cgcacctgga ccacctgaac
     1621 caactgcaac cccgattgag ccgccgaatt tagcttgcat aaccgctgca agagcacctg
     1681 tctcttcgtg gcgaacttgt aagaaacgga tatctttgtc ttcagccaaa gcatccatca
     1741 atgagctgag tgttcctgat gggataccgt agattgtatc tacgccccat gttttcaata
     1801 cgttaagcat tgctgcagat gcagtaattt tcccttgagt cataatgata actctccttc
     1861 aattttttta aacttggaga atacgattac atagaattgg aaacgttctc caaattttta
     1921 ctattccact gtatcatatt tatgctgact tttctaaaaa tctgctcaaa actctctatt
     1981 ctctattcta atacagtttt gaaagtgttg tcgtttctgt tttataacaa agaaatctag
     2041 tcattacttt tagtctattt tactaaaatt taacagaagg gaactggtca gaacagatac
     2101 agaactaaag gccatggcta gacctgccaa ttctgggttg agagccagtc caacacctga
     2161 aaagactcct gctgcaatcg gaattccgac aacattgtag ataaaagccc agaaaagatt
     2221 gagtagaatt cgatgaaagg ttttcttact catatcaaag gcacgaacca ctcctaaaag
     2281 attattggtt gtcaacacca aatctgctga ctcgatggcg atatctgttc cagcacccat
     2341 agcaattccc acatctgcta cactaagggc aggagcgtca ttgataccgt ccccaacaaa
     2401 ggctactttc cctgactgtt gcagtttatg gatttcatgg gctttttctt ctggcaagac
     2461 gcctgcaatg acctcttcaa ttccgatttg atctgcaata gcacgcgcca caccagcatt
     2521 gtctcctgtc agcatgactg ttcggagacc acgttttttt agctgactga tggctagctt
     2581 agcattttcc ttaggaatat cttgcaaagc aagcaagcct ttgatttcat tgtcaacagc
     2641 taagaacaca actgtcttag cttctttttc tagttcttct agtttatctt gataagtatt
     2701 agaaatatcc atgccatcca gcattttagc atttccaagt aaaacttgtt ttccattgat
     2761 tcgccctgaa acacctttcc cgtgcaaggt ctgaaaattt tcaacagttt gaaactcaag
     2821 tccagcttca ctcgctcgct taacgatagc ctcagccagt gggtgttgag aagcatcttc
     2881 caaggaggct gccaacccaa acacttctac ttcgtcgccg atgacatctg ttaccacagg
     2941 tttcccttcc gtcaaagtcc cggtcttatc aaagacaagg gtttgaactt tctggatttc
     3001 ctgtaagaca gttccatttt tgaggagaac ccccatcttg gcactacgtc ctgtccccac
     3061 cataagggct gtcggtgttg caagtcccaa ggcacaagga caggcgataa tcaaaaccgc
     3121 cactccgtag agaagagagg acacaaagct agctccaagc acaaccacac tatccctgag
     3181 caagacgaac caaacccaaa aggtcatgat tcctaaaatg acaactactg ggacaaaaat
     3241 ccctgaaatc ttatccgtca agtcctgaat cggcgcacga cttgtctgag ctttcttcac
     3301 aaaatccaca atctgagcca aaacagtctc tgagccaact ttttctgctc taaagacaag
     3361 cgttccacta tgattgatgg ttgagccaat gacagtatct ccaactgtct tgtccacagg
     3421 cagactctca cctgtcacca tggattcgtc aatactagag acaccttcta ctacgacacc
     3481 atcaacagca atcttttcac cgggacgcac tcgaatcagg tcgcctacct tgacttgttc
     3541 caaaggaact tggacataac tatcatcact caagacttct gcggttttag cttgcaagtc
     3601 cagtaatttc tccacagctt gggacgtatt ttttctcatt ttttcctcaa aaactgctcc
     3661 caaaagaacg aaaaagagga taaatccagc actttcgaag taaacaggga gaccagcaaa
     3721 gagagcaact aggctataga aataagccac tagagttccc agcgcaacca aggtatccat
     3781 gttggcattg tgctttttaa aactggccca agcactctgg atatatggct tacctgcaac
     3841 taacataata ggcgttgttg ctagaaaggt tccccaatgc atgacttgat gactaatgct
     3901 acctgtcaac atcccaatca tgagaatcac aagaggcaca gtaaagatac tagtaatcca
     3961 aaaacgttgc aggagagata gagattttcg agtcttctca acgactgtat agcttccctt
     4021 ttgcatcttc atgccacaag aaaattcatg tcgccctaat tcttgaggtg taaaacgaat
     4081 gactttctcc tcatctacgc cgattggttc caagatacct tcttcttcaa acagaatttc
     4141 cttataacag tttgaaggag tagcacgatg aaaggtaatc tcagctggaa ttcccttttg
     4201 aagctggata tgggctggat gatagccttt ttcagatcgg atacggattt tttgaatgcc
     4261 attttctaag cttgctttca caatttctgt catagtctcc acctactcta caatcatctt
     4321 gccgtgcatc atgttcatac cacaagcaaa gccaaattct ccagcctgtt caggcgtgat
     4381 ttccactaca tactcttccc ccattggcag gttcgtatgt acaccaaaat ctggaaaaac
     4441 aatttgatcc agacatggtg aaggatcctt gcggtcaaag acaatgcggg ctggcactga
     4501 tttcttgagg acaatcaact caggagtata gcctcccatg acttccactc gaatctcttg
     4561 gtatccgttt ttttgctggg ctttttgtcc agatttttca ggctttttga aaaaccaaaa
     4621 caagataaac gcgataaggg caatacaaat aatggttaca atactattta acatgacgtc
     4681 tcctttacat acaattacat cttacttctg ttacagcact tgatttcttc tctgaaatca
     4741 cagcttccaa gtcttccaag tcagtctgag taaattcaca ttctacaatc aagtcagcca
     4801 acaaattcct aatcctacgg gaacaaacct tgtctttgat atcttggaca agtaaatccc
     4861 gactttggtc tagagttaaa agggctgaat aaacaaagga cttgccttct tttttccgag
     4921 tcaaacactc tttctcaacc agacgagcca aaagtgtctg aaccgtggac ttggaccagt
     4981 caaaccgctc tgccaaaacc ctaatcaaat ctgtactggt ctgctccccc tgcatccaaa
     5041 taatcttcat gacctgccat tctgcatctg aaatctgcat taccatacct ccaaaatcta
     5101 catttgtcaa ttacactcat cagtatactc ttaaaatcta catttgtcaa ttatagaaat
     5161 aatattttct tcgaaaaata gaattttaat catttgaaaa acgatttgca gtcaaatatt
     5221 actatataaa caataaaaat atgctatact aaagaaaaaa gaaaacaacc actaggggtg
     5281 cgtaaagctg agattaacga ctgttagatc cctctgactc aatctaggta atgctagctg
     5341 atggaagtgg aaatgataat ggggactagc agtcttctat tgcctttcta aaacagacta
     5401 gcttgttctt aagaatacaa acttcagttg gttgggaggt tttagatgac ttatttaccc
     5461 gttgctttga ccattgcagg gactgaccct agtggtggtg ctggcattat ggcagattta
     5521 aagtcattcc aagcgaaaga tgtctatgga atggctgttg taaccagtct tgtcgctcaa
     5581 aataccagag gtgttcagct aatcgagcac gtttctcctc aaatgttgaa agcccaattg
     5641 gagagtgtct tttctgatat tccacctcag gctgtaaaaa ctggaatgtt ggctactact
     5701 gaaatcatgg aaatcatcca accctatctt aaaaaactgg attgtcccta tgtccttgat
     5761 cctgttatgg ttgctacaag tggagatgcc ttgattgact caaatgctag agactatctc
     5821 aaaacaaact tactacctct agcaactatt attacgccaa atcttcctga agcagaagag
     5881 attgttggtt tttcaatcca tgaccccgaa gacatgcagc gtgctggtcg cctgatttta
     5941 aaagaatttg gtcctcagtc tgtggttatc aaaggcggac atctcaaagg tggtgctaaa
     6001 gatttcctct ttaccaagaa tgaacaattt gtctgggaaa gcccacgaat tcaaacctgt
     6061 cacacccatg gtactggatg tacctttgct gcagtgatta ctgctgaact agccaagggc
     6121 aagagtcttt accaggcagt tgataaggcc aaggccttta tcacaaaagc tattcaagat
     6181 gcccctcaac tcggtcatgg ttctggtcca gtcaaccata caacttttaa agattaagaa
     6241 aaaaactctc tagttcccac tttaagggaa ttagagagtt tttataccct tcgaaaatct
     6301 cttcaaacta cgtcagcttc catctgcaac ctcaaaacac tgttttgagc tgacttcgtc
     6361 agtcttatct aaaacctcaa ggcagtactt tgagcaacct gcgactagct ttctagttta
     6421 ctctttgatt ttcattgagt attaattagg aaagaatgtc atgcaacttt gttaaaaagg
     6481 cttgcgtttt tgcctcaata tcttctgctt gcatcaaatc acgtacaaca gctacaccag
     6541 ctatgccagt gcccataagc tgatcaatat tctccgaagt caagccgcca atagcaacta
     6601 ttggaatggc aaccgtttgg caaattgttt tcaaggttga aatcagggtg atgggtgcat
     6661 tttccttggt ggttgtcgga aaaatggctc ctgtccccaa gtaatctgca cctgatttct
     6721 ccgcttccag agctctttta accgttttag cggtgacacc gaggattttt tcaggaccca
     6781 agacttgtcg ggcaaccgaa actgggagtt cgtcatctcc aatatgcaga ccagcagcat
     6841 caaccgcaag acaaacatcc aaccgatcat cgattatcaa gggtacctga taggcatctg
     6901 ttatttcctt gacttgtttt gccagttgat aatattgatt ggttgtgaga tttttttctc
     6961 gcaattggac tatggtaacc cctgaacggc aggccgtctc aacttttgca agaaagcttt
     7021 ccacggaatc ttgatagcga ttggttacca gatatagtct aagtgcttct ctattcataa
     7081 gcctctcctt tgatggtatc tagccaattt tcatctcttc ttaggagcga aagctgattg
     7141 agtacttggt aacgaaattc ttccaatccc attccttgaa caactatttt ctcagcagcg
     7201 atattgagat aagagactgc taagcaagaa gcttcaaaac cagtctttcc ttggctgaga
     7261 aaaacggctg ttaaggctcc aaccaaatct cctgtccctg ttatccagtc taattcagta
     7321 cagccattct ccagtacagc gacctgattt ttcgaaacga tgaggtcctt ggggccagta
     7381 actaagaatg acataccagg ataggtctga caccagtctt tcaagacttg aagcaaatcc
     7441 tccgtttctt gatctttagc actcgcatcg accccaacgc cgtggtgctt taagccaaca
     7501 agacttcgaa tttctgacat gtttccttta atgaccgtag gtctatagtt taaaaggtct
     7561 ttaactaagc tcttacgaat ggatgaagtc gttacgccaa ccgcatctac taccatcggg
     7621 agagaagatt ggtttgcata cgaagctgcc atgcggattg ctttttcctt ctcagctgac
     7681 aaatgcccca aattgatgaa aagagcctga ctttgcttag taaaatcaag aacctcacgg
     7741 ggatcatctg ccatgacagg tttgcatccc agagccaaaa tcccatttgc cagcatctca
     7801 caagaaatct cattggtcat acagtgaatt agggaactag agcctatagg aaagggattt
     7861 gtaaattcct gcatcagtct atcctttcac taaagaaata tccctgcact tttttaaaga
     7921 attcctgctt gattaaaaat cgaaaggcaa taaaggaaat cgctgtacca atcaaggttg
     7981 ctccgaaaaa tcgaggcgtg tagataaacc agctaagctt agcagctgat cctgtaaaga
     8041 gtaccataac aggataggaa acaatggaac caataatacc tgttcccaca atttctccca
     8101 aggcagaaaa ataaaatttt cgaccgtact tataaaagag acctgccaga agggctccaa
     8161 aagtcgctcc tgtgagagct aaaggcggaa tcccttgagt cgtcatacgg ataaaggctg
     8221 tgactgtagc catggccaag gcataaacag gtcccatcat gattcccgct agaatattga
     8281 ctacactgga catcggtgcc attccctcaa ttcgaaagat aggtgtaagg actacatcaa
     8341 gggcaatcat catagataaa atggtcaatt tgtgaacttg tagttggtgc tttctcatgt
     8401 ttctattctt ctcctttttc taaagactgt aaatcgctct tccatgtctg gtgttggtaa
     8461 gccatctccc aaaacttggc ttccatatga acactgatgt ggaaggcatc tagcattttt
     8521 tgcttatctg tctcatcact ttctcgatag agctgattga ccagtgctcc ctcctctctg
     8581 atctgttgct ctaactcatc cgtaatataa gtttcaatcc attgttgata gagaggattt
     8641 ggtgatggtt taagattaag tgatttgcct atatcatggt ataaccaagg acaaggaagc
     8701 aagcttgcaa aagctatggc taagttcggt tctgcaaatt gcctataaat atgagaaatg
     8761 taatgataac aggttggagc aattggatgt tgctccattt cctggtcgct gatttccatt
     8821 tccttgaaaa attgttggcg aataaataac tcaccctcca ctaaaccctg agcattttgt
     8881 ttcaagagtc ttttcatctc ttggtttgaa gtcttatcag ccaaaagatg atagatttct
     8941 gagaaggcct tcagatagta ggcatcctga atcaggtaat agcggaaaat ggcaggttct
     9001 aaattcccct cttgtaattg taaaataaag ggatgatgaa aggaagcctg ccaagctttc
     9061 ttggataatt ccatcgcaat atctgtaaat tccataataa ctcctttata aaaatagact
     9121 ggtttgaagc aataaaaaga aaagcaggta gattaatttt gtttttttag gaatataaaa
     9181 agtccgatag ctattcttca actgtgcatg ttcgtcatat ccatgagcag acagagctct
     9241 caggtaaaga tggcgccacc taaagactgt catcagaacc ttactgtaaa tcaagggcga
     9301 ccaaaaatgc agttcttgac cacgtaatag gcaagcttct ttgagggact tgatttcttg
     9361 ctgaatgaga ggaaaggaat tgaataccac aatcaaggca taggaccaag agcgtgatag
     9421 ccccttttga gccaagtaca agagaagctc ttttagtgaa atagaggaaa caaagacaag
     9481 gccgatacaa actgtcacaa aggccctcgt tccaagcatg actgcctgtg aagcatctcc
     9541 gtgtaactga actgcccagt agttggcaaa agatggtaaa atggcaagta tgatcatcca
     9601 agctaacatt ttaaatcgac ggtaatagag cataaagaga atacaaaatg cgactaccga
     9661 aagattcaga gcaatcgaag gaatgaaaga tgtttccaag gataaaatca gcaagaagag
     9721 actgataatc ggtgtctggg ttgctacttt gaccatacta tctcacctcc ccttgggtat
     9781 tgctactctg agatgtaagt ggtttggtaa tggtcacttc tttcacatgc cgaagaccct
     9841 gactagtcat ctcaatccaa taatcaacca cagaaatcaa agggtctaaa cgatgactaa
     9901 tgagcagaaa acttcttcct tgattcctct cctccacaat ccacttgcaa aaataatggc
     9961 aggctctatc atccaaacct gcaaaaggtt catctagcaa gatcacggaa gccttactgg
    10021 tcaagatggt caagagctga agaatttttt gctgaccacc acttaattga tagggactct
    10081 tatcgactgc ctgctccaaa tcaaaatatc gtaaagcttg aaaaatccgc tgatttcttt
    10141 cagaatcagg tccatctaat tgaagctcct ctcgcagact gactcggata aactgcttct
    10201 cagcttcctg aacaacacca gtcagatcac gatacaaact ctttttcttt ttcaggactg
    10261 tccccttcca tgcaatgcgc cccttatact tttgaaattg aagaatagag cgaaagaggg
    10321 ttgatttccc gacaccattg tcacccagga tacaggaaat cccttgatag aatgtgaaat
    10381 cagcaattga aaagaggggg cgattaccaa gctcaccagt cacacggttc atatggaata
    10441 gttccgggct agaagcaact tcctttgaag caacctgtgt catctcatag gaagggattt
    10501 gaaacacttc ccttagtttt ccgtctctta gctcaaccat atggtcgata taggctttat
    10561 agtcagataa atcatggtcg cacaaaataa ctgtcttccc atcaagagcc aactctttta
    10621 gaatctccaa tatctcgatt ctgctcttgc ggtcaataga agcgaagggc tcatccaaga
    10681 gatagaccct aggattcatg gcaaagagga cagccagcgc tgctttttgc ttttccccac
    10741 ctgataggtg atgaatggga cggtgtaaga ttgcctcgca acgacattgc tggacaacct
    10801 ctgctatttt agaatcaatt tcctgaaggt gatagccgat attctccaag gtgaaaatca
    10861 gctcctcaaa caagttctcc atggtaaatt gatgattggg attttgaaag agaatgccaa
    10921 ccttctggac acgtgcgatg atagaaagct gactgacctc gctcccatct atcaggactt
    10981 gaccactata gggaagagaa ctgacttggg caatgacttg aaaaaggctg gattttcctg
    11041 aaccactgct cccaactaac aaggtaaagg cttgcgcatg aaaagtaaaa tcaaacggct
    11101 cagagaagat tggggactga atcgctcgta gttccagacc catctatgct tttcctccag
    11161 ttgcaaactg atgatagagt ttgacaatgg cacgaaccaa gatggtacag aagaaataaa
    11221 cagaaataaa acgtaccaca agtaaggaaa ggacaaacgg aagggaaaag gcgtagtaac
    11281 ctaacttaat gtattcatag acaaagctaa caagcgtaat cccaatacta ttagcagtta
    11341 gagagagcca actttcatag cgattcttag ttacgataaa accaaattca cttcccaaac
    11401 cttgaaccaa gccagacaaa agagctccta gaccaaattg gctaccataa aggacttcag
    11461 caagcgcagc tagcacttct ccaatcgttg cacttccgac tctcggaaca aagatggcag
    11521 caatgggcgc agccatacac cagagaccga agaggatttc attggcaaag gcctgcaaac
    11581 caagaggtgt taagagtaga ctgagaatat tatacacata tcctgaacca acgaaaaccc
    11641 caccaaaaaa gatagacaag aaagcaagca agataacatc ttttaactgc cattttttca
    11701 acataaaaaa ctcctttttt aaaagaaaag tggggcactc aagaagaccg acctaaatac
    11761 tttgtatagc agattgaatt tagaacagta cacaggaatg ctaaaatatt tctagaaatt
    11821 aatttgaatt ttctaattga tttgttcgca tcttatttca atctactata tcatcttcat
    11881 ccagtttcgt aaaagaaaaa actctaatta cagatacgaa ttagagttca gcttacaaga
    11941 ttagacagtt cttttcgaca tacgaaaaaa acatttcaca tttcccttcg ccagtcttaa
    12001 ctgtatcagg ttcaatgggt atcatctcag cctaaagcac cccaaatgcc tttattattt
    12061 aattatgtga ttattataac acacatttta tactagttca agaaattgaa ctggaaatac
    12121 agccttgcac tcacaaagac agcagatctt tcttttgcaa aaaacaaatg acctgtttga
    12181 tgaattagcc attcaagctg aatctagaca tagcttttta aaaaaggaaa atcctactta
    12241 cttagaatcc aaggatagat atctattgtt cactcatttc ccgaacagtt ttttctatat
    12301 tttttgcata cgatattgcc gaaatgattg aaacgccgtc aacattggtc tttataatgt
    12361 ctttaatatg tttcgtctgt atcccaccaa ttgcaactaa aggcatttgt ggcaatagtt
    12421 ttctcatcaa ttcaagacct tcataaccta tagcaccacc agcatcatct tttgactggg
    12481 tatcaaatac aggcccaaca cctacataat ctacatattc aacttttgat tgttgaaatt
    12541 cttcttcgtt tcttatagaa agaccaatta ttttatctgg catcaatttt ctaatttcat
    12601 caacaccaat atcatcttga cctacatgta cgccatcggc gtcaatttcc attgctaaat
    12661 ctatatcgtc attaacgata aatggaacat tgtatttttt acaaagttct ttaatttgga
    12721 tagctagctc aagtttttct aagccttcta aagcaccctc acctttttct cgaaattgaa
    12781 ataaggttat agcacctttt aaggcttcct caacgactgt atatagattt tttccttggc
    12841 aagtagtcgt tccacaaata aaatatagtt ttagtaattc tttatgaaac atcttacttc
    12901 actcttttga attcctttac atcttcatct gtaatctcgt ataaggcatt tataaattca
    12961 gctttaaatg ttccaggaag atgtccattg ggacgttttt ctgctatttc tccagcgata
    13021 ttgtaaacca acattgctgt ttctaatgat ttcaattctt gacctttttc tagtccgata
    13081 aagcttgcta ctacagctcc taataagcat cctgtcccaa tgactttcgg catcatagca
    13141 ctaccattat gaatcgttac cacttctcca ttaacagcaa tggcatccac ttcacctgtt
    13201 actactattg gaatattgaa cttctcattt gctgctagag caatttcgtc aatattatct
    13261 acgcccgcac tatctactcc tttagatgcc acatctattc ctactaaaga ggcaatctcg
    13321 ccagcatttc ctctaatcgc tgctagttta taattgttga ttagatcatc cgctactttt
    13381 tttctatatt ctcctgctcc acaggctaca ggatctaaaa ctgctgggac attatatttc
    13441 tccgcaattt tcagagcagc ttggtataat ttccaatttt catctgtcaa tgttcctatg
    13501 tttattaata aaccaccagc atactttaac aaatcctcta aatctgctgg aaactcactc
    13561 atggctggtg aggcgcccag tgctactaat ccatttgctg tgaaattttt tactacatca
    13621 ttggttatac aaatgaccaa tggtgctttt tcttttaata attttaaact tgtcatattg
    13681 aaatccttcc ttttcacttt atacgatcta ctgatttcga tttatatcta tctttcgtta
    13741 agaaattttt ttcatttaca ttgaatgatt tatactcaat gaaaatcaaa gagcaaacta
    13801 ggaagctaac cgcaggttgc tcaaaacact gctttgaacc tgtagataag actgacgaag
    13861 tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagt aaccatacct
    13921 acggcaaggt gaaactgacg ttgtttgaag agattttcga agagtcttac ctcatcaaat
    13981 ttgtaaatat catgagcctt ctctagacat tgtaaccaat atcaaaaaaa gctaattcta
    14041 aagcgactgc ttgattccag cgttgctgaa gttctgtcaa atcttctcga tttttaccga
    14101 cacgattgag ttcgtcaacc agaaattgaa cccactctgc aaagaaagga cctctgtgga
    14161 gattgatcca ttccgaatga atatagactt caggtagagc caaatcttta gaaccccagt
    14221 ctaaatagag accttctgca atgaccagca taaccaaaag atgggcatag tctgatgaag
    14281 ccactgccga atacattaga tcctgaaagg cttttgttac agggtgcaaa gtcacttcta
    14341 gatagtcatt ctctgctact tttaactctt taaaagcctt ttggaaataa ccatcttcat
    14401 ctgcttcaag aaagcctagt tgcttggcaa aacgaagctt ggattcaagt ttatctgcgt
    14461 gagctacgca ggcacccagc atggataaga aggcatcaaa gaagtgataa tcttgaatca
    14521 gatagtcctt taagacctta ttctcaattg tccccgcaaa aagttcctta acaaaacgat
    14581 gattgattgc agcctgccaa tccttctgac tgctttttaa taattctcca acagtcaaac
    14641 ctggctgaaa tgcatagtct tgtgtttcca tatttacttc tcctctcttt acttgttagt
    14701 aattaataaa acaccaagaa atatcaagca aaatcgtaat tccacttgat ccttttaaag
    14761 cacatcgaga gcatttgcag agagctaact aaacaagcct atccagttta tataaacaaa
    14821 aaactccaat tacaatcaag aattagagtt gacttacaag attagaccgt tcatttcacc
    14881 atacgaaaaa actgttcaca tttcccttcg ccagtcttaa ctgtatcagg ttcaatgggt
    14941 attatctcag cctaaagcac cccaaatgtc tctattattt aattactgaa ccagtatagc
    15001 aaaaaatgaa agccctagca agatatttga ccgaaaaata tctttatata taatatattg
    15061 aaactagaat agtacacctc tacttctaaa acattgttag aaatcgattt aactgtcctg
    15121 attaatttgt cctattctta tttcatttta ttatagtttt cgatagcgat ttattcttcc
    15181 aatacacgaa gaaaaacctc cacattcagt ggaggcaatc tgttttatca atacaatttt
    15241 aagtcacgag ggtcaactgg gaaggttggg ttgtatggat tgtgacggag cttaaagtgt
    15301 ttgacatctt caatggtctg agctccagac aattgcataa ctgtcttcaa ttccgcattc
    15361 aagtgttcaa agacttgacg cacaccgaca ctaccaccga gagccaagcc ataaataaca
    15421 gggcgaccaa tagctaccaa gtctgctcct gaagccaagg ctttaaagac gtgttgacca
    15481 cgacgaacac cagagtcaaa gacaatcggc acacgtctat caactgcttc tgccacttct
    15541 tgaagcgagt caaaggcagc tggtccaccg tcgatttgac gaccaccgtg gttggttacc
    15601 cagataccag aagctcctgc agcaagtgaa cgttcaacgt cctcacggca ttgtggtccc
    15661 ttgacataca caggaagacc agagtattca gcgataaatt ctacatcgcg tggagacaag
    15721 cgttgtttag ctgatttgta aacaaagtcc attgatttac cagcaccttc tggcaggtat
    15781 tcttcaacaa tcggcatgcc aactgggaag acaaaaccat tacgcttatc cacttcacga
    15841 ttccccccta cagtagcatc tgccgtcaag acaatcgctt tataaccttc agccttcaca
    15901 cggtccatga tgtggcggtt gataccgtca tccttactaa agtaaaattg aaaccaatga
    15961 ggtgtccctt gaagggcttc agaaatctct ggaaggtcaa cagtagagta agaactggtt
    16021 gtataaagag aaccaaactc atgcacacca cgcgcagtcg ccacttcccc ctgttcattc
    16081 gctaatttat gggccgcaac aggcgccata atgattggtg aagatagttt ttcacctgca
    16141 aattcaatct ctgtacttgg attttcaaca tcgcaaagtg tatgaggaac gatgagtttg
    16201 tggttaaagg cgcggatatt ctctcttaaa gtgaaagtat cttccgcccc actagcgata
    16261 tagccaaatg ctgctttagg aataacttgt tgcgccattg gctccaaatc ataggtattg
    16321 atgaagtcta catgaccttc tgcattgctt gttttgtatg acataaaatg ccctccttga
    16381 ttaagtaagc gtttgctttt tactacacaa ctataataac tcttttttca atcattttca
    16441 agagttttgc ttaaaaacca tcttattttt tcatacaata tactagccct actttcctta
    16501 aactctaaac tttagtctat attaagtgaa tcgttgagat tttttgctgc caggcatttg
    16561 aactactttt ttatttgcgg attctatcta ctaatactat aaaaaaacaa gtgaataaga
    16621 caccagaaca tcctctaata ggacctatgg acttatatca gtcccatata tactgtttaa
    16681 gcaagatact atcctagttg acgctaactt cctatcccac ttattaattt tttatttata
    16741 gtgaatctga acaaatcaat tgggaaatcc aagtcaattt ctagtacttt ttgaacagtt
    16801 tcagcgtact atcgtaaaat gaaataaaac atgcacaaat tgattaggga atttaaacca
    16861 atttctaaca gtgtcttaga aatcaaagtg tactatttca acttcaatat actacacttc
    16921 attttctcct taaattcagt ctctttttga atcaaaaaat cccctggtaa gagccagagg
    16981 gtaagaattt atttcattgt tgggaagagc aatacatcac ggatagttgt tgtatcagtg
    17041 aggagcatgc agagacggtc gataccgatt cccaaaccac ctgttggtgg cataccgtat
    17101 tcaagagcct cgatgtagtc atagtcgatg cctgtcgctt catcatcacc aagttcttta
    17161 gctttagctt gggcttcaaa acggctaagt tgatcgattg ggtcgttcaa ctcagtaaag
    17221 gcattaccgt actccttagt catgataaag agctcgaaac ggtcagtaaa gcgttggtct
    17281 tcaggatttt tcttagcgag tggagataca gctactggat gtccatagac aaaggttggt
    17341 tggattaaag tttcttcgac aaactcttca aagaaggcat tgatgatgtg accaacctca
    17401 gtgtagtgtt tctcaactgg aactttcttc tcagcagcga tagctttagc ttcttccaaa
    17461 gtcatgtctt gccagaaatc gacaccagta atttctctga tagcatccac catatgaaca
    17521 cgcttaaatg gtttgttaat cttgatttca gtaccttggt agttgactgg gccatcacct
    17581 ttgactgatt tagcagcgtg ttggataatg ccttccgtca agtccatgat gtcttggaaa
    17641 tctgcataag cttggtaaac ttcgatagaa gtaaactcag ggttatgagt agcgtccatt
    17701 ccttcgttac ggaagatacg gccaatttca tagacacgtt ccataccacc cacgataagg
    17761 cgttttaagt gaagctcagt cgcgatacga agcaccatgt caatgttttg ggcattgtgg
    17821 tgggtgataa atggacgggc agcagcacca ccggcttcat tatgaagaac aggtgtttcc
    17881 acttcaagga atcctttttg gtcaaggtaa cgacggattt cagagatgat ttttgaacga
    17941 gtgacaaagc gttcaaagct ttcacgatta gaaatcaagt caaggtaacg tttacggtaa
    18001 attgtttcaa cgtctgtcaa accatggaat ttctcaggaa gaggacgaag agccttagac
    18061 aagtgtgtga tgtgggttgc cttgatagag agttctccca tatccgtacg catcacttca
    18121 ccttcgacac caaggaagtc accaaggtct gcttttttga agatttcgta gttttcttca
    18181 ccgacagcat ccttacgaac atagatctga atctggcctt cgcggtcttg aaggtgggca
    18241 aaaccaactt ttcctttacc acgtttggtt accaagcgtc ctgcgatagt agctgtttcg
    18301 tttttatcgt gtaattgttc tttatcgagg ttggcatatt tatcttttaa ttcttgtgaa
    18361 tttgcagtac gttcaaaacg ttttccgaaa ggatcgattc cttgttcgcg gagcgcagcc
    18421 attttttcac ggcgaacgat ctgctggtca tttagttctt ccatatgttc tgtagacatg
    18481 ggatcctcct agttttactc aaaattgaat acgatgagtc attttttgcg atacccccat
    18541 tttatcataa ataagcaaga aattcacgga taatctttaa aaactaaaaa gaacctgacg
    18601 ctaaaatcac tggaaagaag tgaaagtatc ttccgcccca ctagcgatat agccaaatgc
    18661 tgctttagga ataacttgtt gcgccattgg ctccaaatca taggtattga tgaaatctac
    18721 atgaccttct gcattgcttg ttttgtatga cataaaatgc cctccttgat aagtaagcgt
    18781 ttactttgta tattgcagtt tggggaagtt tgggatcctt tctgctcggt ttaatcgtta
    18841 gtatcatccg acattatcga atccctgttt tggcgcaagt agcgacagcc tacattgaat
    18901 tgtcacgtaa tacgcccctt ttgattcaac tcttctttct ctacttcggt cttccccgaa
    18961 tcgggattgt cctatcttca gaagtctgtg caacgcttgg gcttgtcttt ttaggaggct
    19021 ccgccatgca ggattcggga atccaagtac tctttcaagg aaataacctc ctgagaatct
    19081 tacagggatt gggcgttacg attgggatat ccatcctgtc tgtcctctta tccatgatgt
    19141 tcggaacagt catgggaatc atcatgacct cccattctag aatcatacga tttttaacac
    19201 gattgtatct ggaatttatc cgtatcatgc cccagctggt gctactcttc atcgtttact
    19261 ttggcttggc tcgaaacttt aatatcaata tctcaggtga gacttcagtt attatcgttt
    19321 ttaccctctg gggaacagct gaaatgggag acttggtacg tggagctatc acttctctcc
    19381 ctaaacatca gtttgaaagt ggacaggcac tcggcttgac taatgttcaa ctttactacc
    19441 acatcatcat cccacaagtc ttgagaagac tgctaccgca ggctatcaat cttgtcactc
    19501 ggatgattaa aaccacttca ttagttgttt tgattggggt tgtggaagtg accaaagttg
    19561 gacaacaaat catcgatagc aatcgcctga ccatcccaac tacttcattt tggatttatg
    19621 gaaccattct aatcttatat ttcgcagttt gctaccctat ttccaaacta tccactcact
    19681 tagaaaaaca ttggagaaac taaatgtctg aaactatctt agaaatcaag gaactaaaaa
    19741 aatccttcgg agacaatccc atcctccaag gactttctct agaaatcaaa aaaggggaag
    19801 ttgttgtcat cctagggcca tctggttgtg ggaaaagtac cctccttcgt tgcctcaacg
    19861 gcttagaaag tattcaaggt ggagatattc ttctggatgg tcagtctatc gttgaaaata
    19921 aaaaagattt tcacctagtt cgccaaaaga ttggcatggt cttttaaagt tatgaactct
    19981 ttccccatct ggatgtctta caaaacctca tcctaggccc tatcaaaact caaggatggg
    20041 acaagaaaga agtaacggaa gaagctttgc aattactaga tcgtgtcggt ttgctggata
    20101 aacaacatag ctttgcccgt caattatctg gtggacagaa gcaacgtgtt gcaattgtcc
    20161 gtgccctcct aatgcatcca gaaatcatcc tttttgacga ggtgactgct tcgctggatc
    20221 cagaaatggt gcgtgaggtg ctggaactta tcaatgattt ggcccaagaa ggccgtacca
    20281 tgattttagt aacccacgaa atgcagtttg cccaagccat tgctgaccgg attatcttcc
    20341 tcgaccaagg gaaaatcgct gaagaaggaa cagctcaagc cttctttacc aatccgcaaa
    20401 ccaaacgagc ccaggaattt ttaaacgtct ttgactttag ccaattcggc tcatatctat
    20461 aaaggagatt cttatgaaac tattcaaacc actcttaact gttttagcac ttgcctttgc
    20521 ccttatcttt atcactgctt gtagctcagg tggaaacgct ggttcatcct ctggaaaaac
    20581 aactgccaaa gctcgcacta tcgatgaaat caaaaaaagc ggtgaactgc gaatcgccgt
    20641 gtttggagat aaaaaaccgt ttggctacgt tgacaatgat ggttcttacc aaggctacga
    20701 tattgaacta gggaaccaac tagctcaaga ccttggtgtc aaggttaaat acatttcagt
    20761 cgatgctgcc aaccgtgcgg aatacttgat ttcaaacaag gtagatatta ctcttgctaa
    20821 ctttacagta actgacgaac gtaagaaaca agttgatttt gcccttccat atatgaaagt
    20881 ttctctgggt gtcgtatcac ctaagactgg tctcattaca gacgtcaaac aacttgaagg
    20941 taaaacctta attgtcacaa aaggaacgac tgctgagact tattttgaaa agaatcatcc
    21001 agaaatcaaa ctccaaaaat acgaccaata cagtgactct taccaagctc ttcttgacgg
    21061 acgtggagat gccttttcaa ctgacaatac ggaagttcta gcttgggcgc ttgaaaataa
    21121 aggatttgaa gtaggaatta cttccctcgg tgatcccgat accattgcgg cagcagttca
    21181 aaaaggcaac caagaattgc tagacttcat caataaagat attgaaaaat taggcaagga
    21241 aaacttcttc cacaaggcct atgaaaaaac acttcaccca acctacggtg acgctgctaa
    21301 agcagatgac ctggttgttg aaggtggcca ttaactctta aaagggactg gattttaagc
    21361 tccaatccct ttttaagatt ttacctataa catcctgagt ctatctaaga tgttcaatct
    21421 gaacacagtg tacatacttt atcttctatt gcatatactt tatcacataa gatacgaata
    21481 tcctcttcac tatgactagc aatcaaaatt gttgtccctt tttcactaga gagctttcta
    21541 aacaatgttc tcatattttc tacacttgat ttatccaagg cattcatagg ttcatctagt
    21601 aaaagaatag agggattctc cataattgct tgagcaatcc ctagcttttt cctcatacct
    21661 agcgaataag ttttaacttt ctggtctttt tgctcatata gaccaactat tttcagtgta
    21721 tcattgattt cctgattacc aactactcct cgtatgcttg ccaaatattg taaattctta
    21781 aagccactat aataatttat aaaaccaggt tcttcaatca aagctcccaa attagctgga
    21841 atttttctct caggaacaat attttcccca ttgattaaca cttctccata agacggacta
    21901 tataaaccag ctattaattt aaacaataca cttttccctg agccattcgc accagtgatt
    21961 cctatgattt cccctggttt acaactaaag ttaaggtttt gaaaaacaca tgtctttttt
    22021 aatttcaact caatattttt taatatgatt atttcattca ttctataaac ctcctctttt
    22081 gacgagtgaa atagaaaata cttttaaaaa gaaagactaa aaatagcaac tgaagaaata
    22141 aatctcgtcc tatatctcca ttccctcgat tcaaaatata aaatagataa ttagttcgat
    22201 tccctacaaa tagaccacca aacacaatca tgagtaaaaa gaaactaacg caagcaaagt
    22261 tcatttgtac attcgctcct agtgcgtata acataaagca aagcaaggat agcacacaag
    22321 cattcaaaga atcaactata aacaaaactc ctatatccca gtttggtaac tgtaacataa
    22381 attgaatcac tagaactttt accatgatta aaatgagtcc ataaagaaaa caggtccaaa
    22441 tctgatacct aatccagagc ttaatagaag aaaatcgaat cacttccata aagaaatcgg
    22501 atgctcttct acgatataca actgctagaa aaaatgtatg aataataaac caatgaagca
    22561 accatatgga taagactaaa aaagagtttt gactcagatt aggccctttt aaaatttcta
    22621 taacaagtct atcatttcct tctaaccaat accataatcc taaacaaatc aatatccaaa
    22681 aaagaacata accccaaaag tacttggtat agattatctg attcaacctc cacatgtaac
    22741 tacttaactt tcccatttcc aacttttaca ctctcctcca gtttctatga acagaaaata
    22801 cagtcaatgg aattatgata gatagtaatg taagccaata aatcaatgca tacggattgg
    22861 aatttaccat atactgaaaa attggcaata atccaattat cccattgtcc aactgataaa
    22921 tccagaataa ggattctaca aataaaaaag taaacagaaa tactaagctc atgtttgatt
    22981 ttttaaacca agaaaaactg ctaatagtaa gtaaggataa aaagaaatag tatgctatat
    23041 aagagaaaaa aaatcctata aagaaactag cattgtttgc aatacttata ccataaaatt
    23101 ctcttaaaaa atcaacctcc tttatctcca aagagaagct aaaaccatta ctaaatgcaa
    23161 ttagaaaaat caataaaaat aaagtcgccg tccaaatccc cgtactaaga gctgctaatt
    23221 tgaaactaaa actggtaaag tgcttaattg atttcagacg aatgcgacac tccaacctat
    23281 taaaatagtt attcatcaaa taaaaaaaga acaatatata tgtgaacgga aagcaatata
    23341 ctccagtcgt catatcttga agtaaaacta agatccattc taatacattt ggatggattg
    23401 aatattggcg acagcgcaat aaatatactg tactagataa aacacaggat agcagtaata
    23461 taaaataaac caatactgct aagaaatctt ttttataaaa ttgaacaaat tgtttcatta
    23521 tacgtagtcc tctgaatgta gaaaaaatgt accataaaca accaaacaac taacaaataa
    23581 aataaaagca agatgcccac taactaagga aagactgata tctttctgat atcccaaagc
    23641 taatgttgtc acaggttcta agtaagatag ccctaaaata gcccaaaaaa taccaccaac
    23701 catcatatag gcaactggga tgaaaatagc tcctattttt ttcttcacta gcaaagcact
    23761 agctagtcca aaaatagaga acacagcgcc ccaaactcca taccagagag tcgtcacaag
    23821 actatagagc aactgattag aatcaaataa ttcttttaag gcaccactat aatctccaat
    23881 ataaatttcc tgataaggag tcactaaaag attaattcct aataataata aataggggag
    23941 aaaaaagact agaaaagaag aaataattgc agtactaccc acaatagcta gatacctctt
    24001 tttagaaatt cggcacaatt gtgctgttag aaaatgactc tcagcatcct ctattatctg
    24061 actagaatag ggcagtgtac agataagtgc agctactagg ctaatcggtg aaaatcctcg
    24121 aatagaagaa gcggcaaaaa aaatcgataa accttcaatt ttataaatac cattgaaagc
    24181 aaggaaattt cctaaactca tgcaaagaag ggtcaaaaat aaagacatat aaaatcgagg
    24241 tgattgaacg actcgtacaa gattacaaat gaaaaattcc atccttactc ctccttataa
    24301 taaaaatagg gtgtagcatt cttttttcat gctacaccca caatcaacca tctttaaggc
    24361 ttactctgac aagtaagtta ataagaatct ggactccaag aacctgaagt atgaattctt
    24421 acatgatttc caaattgtgg cgccatagct aatctagtac cagaaccaat ataattgtca
    24481 ccacctccat tatagtacat gacaatccta gagccagacc ccaatgaata taccggggta
    24541 atatctgacc cactataggc gcgctacgaa taga
//
LOCUS       DAVKMU010000028        24073 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKMU010000028 DAVKMU010000000
VERSION     DAVKMU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.028"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(288..1895)
                     /locus_tag="VW343_001326"
     CDS             complement(288..1895)
                     /locus_tag="VW343_001326"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9755870.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENRN"
     gene            complement(2285..2872)
                     /locus_tag="VW343_001327"
     CDS             complement(2285..2872)
                     /locus_tag="VW343_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9755871.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(3000..3449)
                     /locus_tag="VW343_001328"
     CDS             complement(3000..3449)
                     /locus_tag="VW343_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755872.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3454..3843)
                     /locus_tag="VW343_001329"
     CDS             complement(3454..3843)
                     /locus_tag="VW343_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9755873.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3871..4497)
                     /locus_tag="VW343_001330"
     CDS             complement(3871..4497)
                     /locus_tag="VW343_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9755874.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVFASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4511..5074)
                     /locus_tag="VW343_001331"
     CDS             complement(4511..5074)
                     /locus_tag="VW343_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9755875.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5239..5388)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5448..5951)
                     /locus_tag="VW343_001332"
     CDS             complement(5448..5951)
                     /locus_tag="VW343_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9755876.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6144..6974)
                     /locus_tag="VW343_001333"
     CDS             complement(6144..6974)
                     /locus_tag="VW343_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9755877.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRLLV
                     LIAIFSVFLLYLSEIHFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGAYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6959..8641)
                     /locus_tag="VW343_001334"
     CDS             complement(6959..8641)
                     /locus_tag="VW343_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9755878.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHCPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLRTELVKETPELQSLFRLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKALSSFIQTEGRYLWEGQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEQEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTDPASLLSNPELLVKANLKETSIFNLAKKLDVDPLALTAFYKERREGCKLN"
     gene            complement(8743..9000)
                     /locus_tag="VW343_001335"
     CDS             complement(8743..9000)
                     /locus_tag="VW343_001335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755879.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9007..9555)
                     /locus_tag="VW343_001336"
     CDS             complement(9007..9555)
                     /locus_tag="VW343_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9755880.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9570..10418)
                     /locus_tag="VW343_001337"
     CDS             complement(9570..10418)
                     /locus_tag="VW343_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9755881.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10606..11955
                     /gene="trkA"
                     /locus_tag="VW343_001338"
     CDS             10606..11955
                     /gene="trkA"
                     /locus_tag="VW343_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9755882.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11959..13398
                     /locus_tag="VW343_001339"
     CDS             11959..13398
                     /locus_tag="VW343_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9755883.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMAFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLSLIFIVSLDSNDFLIVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13523..15202)
                     /locus_tag="VW343_001340"
     CDS             complement(13523..15202)
                     /locus_tag="VW343_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9755884.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSVMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            complement(15271..16683)
                     /gene="lacG"
                     /locus_tag="VW343_001341"
     CDS             complement(15271..16683)
                     /gene="lacG"
                     /locus_tag="VW343_001341"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9755885.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVQYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(16693..17037)
                     /locus_tag="VW343_001342"
     CDS             complement(16693..17037)
                     /locus_tag="VW343_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9755886.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(17205..19091)
                     /locus_tag="VW343_001343"
     CDS             complement(17205..19091)
                     /locus_tag="VW343_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9755887.1"
                     /translation="MVIRVFDQQKNTYSSFALEELRYYMNQVFNTDIELVEEKEADIF
                     VGLVNEEDGKDHIFISLNKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFIP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYKDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(19093..20415)
                     /locus_tag="VW343_001344"
     CDS             complement(19093..20415)
                     /locus_tag="VW343_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9755888.1"
                     /translation="MNNILTFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRIFIQRGITIKMPNGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            20583..21806
                     /locus_tag="VW343_001345"
     CDS             20583..21806
                     /locus_tag="VW343_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9755889.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPSVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTATKYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKKFDFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHSQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            22817..23101
                     /locus_tag="VW343_001346"
     CDS             22817..23101
                     /locus_tag="VW343_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755890.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDIEKL
                     LDSKELTHFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            23094..23651
                     /locus_tag="VW343_001347"
     CDS             23094..23651
                     /locus_tag="VW343_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter peripheral membrane
                     component"
                     /protein_id="HEU9755891.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNTHEELWLNNHVANMNKQPPLSLCESLYSFENLTVL
                     VVPIEYVLGMKMMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVLLEG
                     FSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT         7639 a         5182 c         4065 g         7187 t
ORIGIN      
        1 aaattaattt gacttttctg atagagatgt tcacatctta tttcaattca tgatacaatc
       61 cagttcctaa aactttgcta gaaattaatt tggctttccg aattaatttg ttcagatttt
      121 atttcatttc aatatactga taaatccatt cactagcaca gggcaagagg gagtcgctat
      181 tgatttcaac ggtagcaggt acatacagag gactcctacc tcagatatac tgcaatatca
      241 tcgtgtgcaa aaaacctctg agatttttct caaaggttct gattttgcta atttctgttc
      301 tcaaccgctg cagtaacaaa ggcagtgtag agttcttctg gtcggtttgg acggcttgac
      361 agttcagggt gatactgaca agctacaaag aatttatttt caggaatttc cacgatttct
      421 accaaacgat tgtctggaga aactcctgaa aagacaaagc ctgctgcctc aaactgctca
      481 cggaaggcgt tattaaactc ataacggtga cggtgacggc gttgcaccac ttcttgattg
      541 tgataagcag cagcagcctt agagccacgt ttcaacttag acggataaag tcccaaacga
      601 agggttccac ccatatcctc aatatcaatc tgatcacgca tgatatcaat gatagggtat
      661 tttgtttctg gtgcaagctc tgcagaattg gcaccttcaa gacctaaaac gtgacgagca
      721 aactcgatac atgtcaactg cattcccaag cagactccca acattggaac atcattttca
      781 cgcgcatagc ggatggcttg gattttccct tctgtaccac gttgaccaaa accacctggt
      841 acgatgatcc cgtctgcatc agacaagagt tctgctacat tctctgctgt cacatcattg
      901 gcattgaccc aattgatttt aacttctgca tcattgacat agccagagtg tttcaaggct
      961 tcgaccactg agatataggc atcttgcaac tccacatact taccaacaag ggaaatctta
     1021 acttgtttct tgaggttcat gaccttgtcc accatggctg accattctgt catatccgct
     1081 gctggtgcgt ctaatttcaa atgatcacaa acaatttggt ccatcccttg tgcctgcaag
     1141 ttcagtggaa tttggtaaag atgttcaaca tccaacgatt cgataacggc ttctggtgcc
     1201 acatcacaga actgggccag tttattttta attccttgac cagctggctc ttctgtacga
     1261 ataaccaaca tatttggttg gattcccaag ccacgcaatt ctttgacaga gtgttgggtt
     1321 ggtttggttt tcatttcacc agcagccttg aggtaaggaa gcaaggttgt atggatatac
     1381 atgacattat ccgcacccac atctgccttc atctgacgaa gagcctctag gaatggcaag
     1441 gactcgatat ctcctactgt tccaccaacc tctgtgataa tgacatcaga gtcggtcgtt
     1501 agagcggcac gcttgatttt ttctttcaaa gcatctgtga tatgaggaat gacttgaaca
     1561 gttgccccaa ggtattctcc acggcgttct ttacgaagaa cttcactgta aattttccca
     1621 gttgtcacgt tggaatattt gttgagattg atatcgataa aacgttcata gtgacccaag
     1681 tccaaatctg tctcagctcc gtcatctgtc acaaaaactt ccccgtgctg gtaaggactc
     1741 atggttcccg gatcaatatt gatataaggg tcaaactttt gaatggttac tttgagacca
     1801 cgatttttca agagacggcc tagactcgct gccacaatcc ctttcccaat ggacgatacc
     1861 acaccaccag ttacaaaaat atatttcgta gacatagatt cctctttcta aaatgctcaa
     1921 ggtcttgtta actacgaggg gatatagaaa acaagaccct actaataaga ataatctggg
     1981 acgactgcac cagattcaca actaatactc ttcgaaaatc tcttcaaacc gcgtcaacgt
     2041 cgccttgccg tagatatgtg ttcctgactt tgtcagtctt atctacaacc tcaaaacagt
     2101 gttttgagca gcctacggct agtttcctag cttgctcttt gattttcatt gagtataaaa
     2161 tacaagaaga taacttcttg aaaaaacaaa aatagctccc taagaactag ggagccccga
     2221 cctctaaaag aggtgcccaa acaatatgat acctaaaaat aggataattg tcaatagcta
     2281 actcttattc ctcgctggtt tcagcgtcgt catctgaaaa ctcgtcgtcg tcttcgttga
     2341 gatccacatc ttctcccaag tcatctggag cgatttcgtt gatttctgca tcataagctt
     2401 ccacttcatt tttttcatca tctggatttt catcatcgta tgaaagagct ggatctgctt
     2461 cgtatgcatc ttcgtcttct ggatcatctg cattgtagtc aatggcatct gaatcaccat
     2521 ccataaaggc attgacacgt tttttcttag cttttggtgc tacttcatcg tcgtcatttt
     2581 cttcaagagc gatgatttct tcgtcgattt cgtccacacc ataccatgaa cgaagacccc
     2641 atttgttgtc cccaagtgag atgaagctac cgtcaaagtt caactctgtg tagaacaaag
     2701 gcaaagcttc gcggatatcg ctgtttgatg ttccaaggta gttttgaatt tcgtttacaa
     2761 gatcgctaaa atgcatctca tgatcgcgac cacgaagttc caagatagca cgcgctacct
     2821 caatcataga tagttcactt ttttcttgcc cagcaaatac ttctaattcc aaagcgtttc
     2881 tcctcattta tactactatc gccagagcga acagactctg acctcatttt atcatttact
     2941 ctttatttta cgataatttt gcggaatagt caaaggttaa gggggagaaa gtggcaggat
     3001 tagactaatt ccaatataaa actccttcct ttttctgttg ctccattttc cacaaatcca
     3061 agcgacttga aacacctcct agaagcatga ttgtaggtgt agatttcctt gactctcaat
     3121 tctttccatc cttttactcg agccaattca atcaaagcac ttagaatctt ttttccaagt
     3181 cctcgatgtt ggtaagcgga attcccaatc acaatgggga gattatcctg agatagtgta
     3241 atatccccaa ttggaaacca ttctcccttc tccttgactt caatccaaaa aagctcacca
     3301 tgccgatcca aataggaata catggcttcc aaggtcgctt gactgtaagg aagcttcacc
     3361 ccatctacga ggtaaaccaa gttcacatcc tgataccaag caaaagcttc ctcacaatga
     3421 ttgaccttat aataaggaac aaaacacaat gcactacctt ttcaacaaaa gagttgctgc
     3481 ttgattaaaa ccatcacacc agttatacca ttttgcttca tacccatctt gagctaggat
     3541 acgatcttct aaatcaaaaa cagagtaaat ctttctttcc tcgcaagctt gcgcatagag
     3601 atgatatagt tcatcaccac catctctatc ccactcagca gaaatcgtat cccgacctgc
     3661 caataaagcc tgataagccc tgtgatgccc atctgtaatc agcaaacaat ctccaaaggc
     3721 aagaatactg attggatcga cttggtttgt ttctgccgac tggtaaagca tctgaatatc
     3781 ttgcaacttc ttttctgata aatatagttg agtcggatga agatctgcta tattgacttt
     3841 catttctttc tcctcaaggg aattcgatac tcacttctgt ttgcctttaa atcgccattg
     3901 gaagcggagc ttgtcataga agggaaactc gataaacagg actcccaagc ccacacagag
     3961 actggcaaag acgtctgatg ggtaatgaac ccccagatag actcttgata ccagcacact
     4021 gactaggtag aggccaagga cgatttgtac gatttttctc cagactggat ttttaattcg
     4081 ttgactgaga atgacaatca gagtacctac catcaaagtt acagccagag aatgtccact
     4141 tgggaaggaa aatcccttct cttccaccag atgtaagata gctggtcgtg ggcgctggta
     4201 gatattttta aaggtcacga ttaaaagacc tgccaaagcc agatttccca gcatgaagaa
     4261 actttctatc ttccatcgct tacgataaaa gacaaaagct gtaatgacaa cccaagtgat
     4321 aatcactggg atatcaatca gacgtgtgag ggctcgaaaa agaatagtca aataatctgg
     4381 taagtctcct cgaatggcag tctgaatcga ttggtcaaaa ttgaccaaca tttcagggta
     4441 aaatttgacc atatagccaa gaataacgaa aagtaaaagg gcaaaactgc ccttcattaa
     4501 aaatgtttgt ttatctttca tagtgtttta aggttggttt caaaaggacg tacaacaacc
     4561 agaatgaaac ggaaaagatt acaccctcaa tcaagttaaa aggtaatacc atggtcatta
     4621 ggtagttgga aagtcccaaa atttttccaa tatcaaagtt agcaaactta gcgtacaaag
     4681 gaacagcata aacatagttg agaaccaaca tggccaaggt taaaccaaca gttccagcta
     4741 gagagcctag taggaaacga agggttgtcc gttccttttt ccaaatcaaa gcaaatacga
     4801 tgacaaaaac tcccaaagct acgatattca tcggcaaacc aatgtaagta ttcactcctt
     4861 ggctgttaag aagcaatttc aatagtgagc gaagcaagag cactcctaga gcagcaggca
     4921 aatccatgac caccagaccc acaaggactg gcaagatact aaattcgatc ttgaggaaag
     4981 atgccgctgg taaaagcgga aagtcaaagt acatcagcac aaatgagatg gctgataaaa
     5041 ttgcaatggt cgaaagtcga cgtgtgtttg tcataacagg ttcctccaat tttctataaa
     5101 atcagaagaa gttggaaagg attcctctat ctattctcac tttttatatc ccaaaaattc
     5161 cctcttactc tattaaagaa aaacaaagca agtggttaca atccggctat aaatctatca
     5221 aaacagacaa agctattctt tcgtcttctc ccatccagac tatactgtcg gttgtggaat
     5281 ctcaccacat cagcttgcgc tcgcggactt ctttaaagag aaggaaaaga tcatttccta
     5341 gttaaagtca ccgccggtcg ggaatcacac cctgccctga agactctttt atcataacaa
     5401 aaaacgcttg caagcgcaag cattttgttc attttcattt tcatttttta tttcaattta
     5461 tccacttcat aggtatggac gagctcaaga cctgcaaagt tctgctggcg aagagcctca
     5521 tagacaatca tgcagacggt attagacaca ttgagactgc ggacatgttc atcattcatg
     5581 ggaatacgga gagccttctc aggatgttct cgcataaaat cttcaggtaa gcccttgtct
     5641 tcacgtccaa agagaaaata atggtcctcg tcagtcgata aatccacctc agaatagact
     5701 ttttctgcga atttcgaaat cagatagagt tttcccttca tctgagatat gaaatcttcc
     5761 aaactctcgt aaaaataaat ctctagctta tcccagtaat ccaatccagc ccgcttcatc
     5821 ttgcggtcat caataggaaa gcccataggc ttgatgatgt ggaggggaga attggtcgca
     5881 gcgcaagtac gcgcgatatt gcctgtattt tgtggaattt gaggttcaaa taatacaatg
     5941 tgatttgtca tgacttgctt cctttcacca ttgcaaaaaa atagccacac tgcccggagt
     6001 caagctcagc aaacagcgtg gttaaggcat cgttaactta cctcacaaca ggtttgaagt
     6061 aaatcagcga aactactttc ttagtataac actttcagaa tcattgtcaa tagaaatgac
     6121 ttgatttttt caattttttc aagctatttc caagggttgt aaaatcgtcc ctgattctgc
     6181 aagataagta gtaaactaac tactaaaaac aaggttgcca agagcaaggt aatatagtct
     6241 ccttttttca aggcctgata actataccat gtgcgttttt tctctttccc aaagcggcga
     6301 agctccatgg cagtcgcaat ggtatcaatg cgttctagcg agctaaaaat caagggagta
     6361 ataatgagca gattgccttt gattcgttgc ataagagaag ctttcttgga taattccatc
     6421 ccacgcgctt cctgagacat cttgatggta aagaattctt cctgcaaatc tggaatatag
     6481 cgcaaggtta gactgacaga ataggcaatc ttgtaaggca caccaatttg atttaaactg
     6541 gaagcaaact gactagggtg ggttgtcatc aaaaagataa tagccagagg aatagtgcaa
     6601 aggtacttaa tagccaaatt tagcagataa aagagctctt gactggtcag agtgtaggca
     6661 ccgattccct gccaaatcac acttctctct ccgtaaagtc caaccccata ctcgggagaa
     6721 aagagataga ccatcaaaac gtttaaaacg gcaaataccg tcgcaaaaac ggctacaaag
     6781 gaaacatctt taaaatggat ttctgacaaa tagaggagaa agaccgaaaa gatggcaatc
     6841 agaacaagca gtctggtatc atagctaatc atggccgcca atgacacgag aatgaaaaag
     6901 aggagtttcc cagcgcctga caagcgatga atcacagtat ctctatgctg gtaaccgatt
     6961 aatttagctt gcatccttct ctcctttctt tgtaaaatgc cgttaaagca agtggatcca
     7021 cgtcgagttt cttagccaag ttgaagatag aagtctcttt taggttggct tttactaaca
     7081 gctcaggatt gctcaacaga ctagctggat cagtatcagc aatcaattcc ccatccacca
     7141 tgacaagggc tcgatctgaa taatccagca tcaattgcat atcatgggta atcatgacaa
     7201 tggtatgccc ttgttgatgc agttcttcga gaaattccat aatctcagta tagttcttct
     7261 ggtcttgacc cgcagtcggt tcatctagga ggataatttc agcgcctaag accaaaattg
     7321 aggcaatagt cacacgtttt ttctgaccaa atgacagggc agaaatgggc caattacgga
     7381 attcatagag accacagatt ttcaaggttt catagactct cgtttcaatt tcctgctcgt
     7441 ccacacctcg caaacggagt cccagagcca cctcatcaaa aatcatattg gttgaaatca
     7501 tttgattggg attttgcagc acatagccta ctcgttccgc ccgctctgca acagaatctc
     7561 cttttatatc ctgtccttcc caaagatagc ggccttcagt ctggataaag ctactcaagg
     7621 ccttggctag agttgacttc cctgctccat tttttccgac aatggcaatc ttttcaccct
     7681 ttttaatatc taaatgaaga gattttaaaa tcggtctatc atcataagaa aacgacacgt
     7741 cctctagtct aaagagtgac tgcaattctg gggtttcttt taccagttcc gtccgcaact
     7801 gaacctgacc ttttgagata gacaagttat ccagatctgc taattgttct tccttgacta
     7861 aatccacacc caattgacgg agagtggtga gataaagggg ttcgcgaatt ccattttggg
     7921 tcaacaaatc agtcgccagt aactggtcag ggctcccatt aaaaaggata cgaccatcgt
     7981 ttatcaagac aatccgatcc acagggcaat gcagaacatc ctccaaacgg tgctcgataa
     8041 taagggtcgt cgtcccctcc tccttatgaa tctggtcaat caattcgata atatcctgac
     8101 ctgacttggg atcaagattg gcgagtggct catcaaacaa gagaatcgga ctttcatcaa
     8161 tcaagacacc agccagactg actcgctgct tttgtccacc tgacaaatcc tgagggcgct
     8221 gatccagtaa aggaagaagg tccagctttt cagcccattt ataaacacga cctttcatct
     8281 catctagggc tgtcacatca ttttccagag caaacgccaa atcttctgcc acagacaagc
     8341 caataaactg cccatctgta tcctgcaaaa ctgtgctaac cagatgagac ttatcataga
     8401 tgctcatatc aaaggctgtt tgacccttta tcaaaaattc tccatatgtc tgacccttgt
     8461 aaatattggg aataatccca ttcaaacact gacccaaggt agatttacct gatccagatg
     8521 gtccaacaat taagactttc tctcccttgt aaatggtcaa gtctatccct tgcaaggtcg
     8581 gttcttgttg tgtttcatac cggaaagaga aatccttcca ctcaattata gcttctttca
     8641 tcttactctc ttcattcgct tcttagactt ctattttatc ataaatcaag cccttcttgc
     8701 agtctcttct cttaaaatct tagcgccaaa aagattccta tcctagctta cttgcctaac
     8761 taatctataa acatcgaaaa agactagttg cccagccttc cccatcattt tatactcttc
     8821 gaaaatctct tcaaaccacg tcagcttcgc cttgccgtag gtatggttac tgacttcgtc
     8881 agtttcatct acaacctcaa aaccatgttt tgagctgact tcgtcagttc tatccacaat
     8941 ttcaaaacac tgttttgagc aacctgcggc taacttccta gtttgctctt tgattttcat
     9001 tgagtattag tcctttttca aacttcctgc acgagtttgg gttcctgcat aggcaagtaa
     9061 gagaagagtt cctgcaatag ctacagatac accattggca attcccgcaa caatcccttg
     9121 tgcaaatact ttttctgccg cttcttgata aatcacaaca tctccaagtg gtgccaagac
     9181 accccaaaca agggcatttg caagtagttg aatgaggtta aaaataagaa tatctttcca
     9241 gtcaaaaaca ccattgatca cgcgaacgta ctttctaaaa agtcccacaa ctaaaccaaa
     9301 gagtccgcta gcgataatcc aagtccacca tagaccataa ccagcaagag agtccttgat
     9361 tgcatgacca atcaacccga caagcaaacc gataatcggt ccaaaaataa tagaaagtag
     9421 cgcttgtacc gcatactgaa gctggatgct tgtatttgga acaggggttg gaatgttgat
     9481 catcccgatg acgacaaaga gggcagcgcc aattccgaca gcaacaactt gtttaattgt
     9541 aaatttaatt tccatactat tctcctattt tatccttcta ttttctttat ttcaatggtc
     9601 caagatgaac cgacacctac attataggcc ttggcaaagg aaccttggtt gatagccaaa
     9661 cctaaacgat agagagagtt gatgtaaagg atgggttgcc caattctcac atctgcaaat
     9721 gatttgccat agacaacctg attttgatag accagcatat cagcatgata gatggtcact
     9781 tcaaaacgat caccaaattc tggttccagc ttgtaaaatt cttcccgtgt gatagaggtc
     9841 caaagcgaac cgaaacgcac atccagaata tcaatggctc ccttcaccag atgatcttct
     9901 atgatggtcg ctacgactgg aagctctaca atctgttcca cactgagctc tggccctact
     9961 tcctcaaaag taatgtgacc actggccagt ttagcaccag tataggcata gacatcacga
    10021 ccgtggaagg tataagaatg ctctgtgttt tgacgcctat tggccacctc agaaatctca
    10081 cgaatggcta caatgccaac gtgtttcttg ataaaggaaa gcgtcccatt atctggcgtg
    10141 acaatgtatt gattttttgc agtcttggca actacactct tacgtttcga accgacacct
    10201 ggatcgacaa ccgatacaaa cgtcgttccc tcaggccagt aatccaccgt ctgaaagaga
    10261 cgatagctcc cctcaaaaat attataaggc gtgatatcgt gcgtcaagtg atggattttt
    10321 aaggttggag actcttctaa agccactcca atcatagccg ataccgcacc atcaaccaga
    10381 ccaaagtctg attgtaatac cagtaaatta ttattcattt tatctccttg tatatccttt
    10441 atcataccat atttcagaga aagtcgctaa tagctatttc aattggttag ggtatagttt
    10501 taccttatag cgaattttca ctaaaagctc ttgttattag ctagtaggtg cattttttct
    10561 tgaaaaaggg tatgatagtt acatcatgaa aaagtaggtt ttattatgaa aattatcctt
    10621 gtcggagggg gaaaagttgg ttttgccctc tgtcgctcct tggttgcaga aaagcatgat
    10681 gttttgctga ttgagcaaga cgaagctgtt ctcaatcata ttgtcagtcg ctttgatatc
    10741 attggtatcc ttggtaacgg ggccgatttt gccattcttg agcaagccag cgtccaagat
    10801 tgtgatatct ttatcgccct gactgagcac gatgaagtca acatgattgc agcagttcta
    10861 gccaagaaaa tgggagctaa agaaactatc gttcgggtgc ggaaccctga atattctaac
    10921 tcttatttca aggaaaagaa tattctcggt ttttctctta tcgttaatcc tgagctcttg
    10981 gctgcccgtg ctatcgcgaa tatcattgac ttccccaacg ccctgtctgt cgaacgcttt
    11041 gctggtggac gcgttagcct catggaattt gtcgtcaagt ccaccagcgg tctttgccaa
    11101 atgcccattt ctgattttcg taaaaaattt ggtaatgtca ttgtctgtgc gatagagagg
    11161 gatcatcaaa ttatcattcc aagcggtgac atgactgtac aggataaaga tagaatcttt
    11221 gtcactggta accgtgtcga tatgatactc ttccataatt attttaaatc acgcgccgtg
    11281 aagagccttc tcatcgttgg ggcaggtaga attgcctatt atctacttgg tattctcaaa
    11341 gatagtcgta tcgatacaaa agtcattgaa atcaatcctg aaatcgccag cttctttagc
    11401 gagaaattcc caaatctcta catcgttcaa ggagatggaa ccgcaaaaga tatcctgctg
    11461 gaagaaagtg ctcaacacta tgatgccgtt gcgactctaa caggagtcga tgaggaaaat
    11521 ctgattacat ctatgttcct tgacagggta ggtgtacaga aaaatattac taaggtcaat
    11581 cgtaccagtc tcctcgagat tatcaatgcg cctgattttt caagtatcat cacacctaaa
    11641 agcatcgctg tagatacgat tatgcacttt attcgtggtc gagttaatgc ccagtattca
    11701 gaccttcaag ccatgcacca tctagccaat ggccaaattg aaaccctgca attccatatc
    11761 aaggaagcca ataaaatgac tgccaaacct ctttctcaac tgaaattgaa aaaaggggtt
    11821 cttattgcag ccattattcg aaagggcaag actattttcc caactgggga ggatatgttg
    11881 gaagttggag acaagctcct agtaacaacc ttgttgccaa acatcaccaa gatttatgac
    11941 ttgattgcga ggtaagaaat gaataaaagt atgattcgtt acctcctttc aaagttactt
    12001 ttgattgaag ctgttctcct cttggttcct gtggctatcg ctgtctatta ccgtgaatcg
    12061 agccaagtct ttacagccct cttttcgacc atagggattc tcgtattgtt aggcggttca
    12121 gggagtttac agaagccaaa aaatcaacgg atttatgcca aggagggagt cttgatcgtt
    12181 gccctctgtt ggatcctttg gtctttcttt ggtggtctcc cctttgtctt ttctgggcaa
    12241 atccccagcc ttattgatgc cttttttgag attagttccg gctttacgac tactggagca
    12301 agtattttga acgacgtttc agttctcagt cgttccctcc tcttctggcg aagttttacc
    12361 cacttgattg gagggatggg agtgcttgtt tttgcacttg ctattatgga caatgccaaa
    12421 aatagccacc tagaggtgat gaaggctgag gttccaggtc ctgtttttgg caaggttgta
    12481 tccaaactaa aaaacactgc ccagattctc tatctccttt atctagctct cttctccctc
    12541 tttgtcatca tctattatct agctggtatg cctctctacg atagttttgt cattgctatg
    12601 gggacagcgg gaactggagg ctttaccgtc tataacgatg gaattgccca ctatggtagc
    12661 tcactgatta cctatctggt tagtatagga gttttggttt ttggggtaaa tttcaatctc
    12721 tactactacc ttatgctccg tcgggttaag gccttctttg gagatgaaga gcttcgggct
    12781 tacttggtca tcgtgctggt ttctacaggc ttgattagcc tcaatactct ctacctctac
    12841 ccaggatttt ccaaaagctt tgaaatggcc ttcttccagg tttccaacat cattacaaca
    12901 actggttttg gatatggaga tattaccaac tggcccctct tctcccagtt tatcctcctc
    12961 ttcctcatga caatcggtgg ttctgctgga tcaactgcag gtggactcaa gattattcga
    13021 ggcctcatcc tttcaaaaat tgccaaaaat caaattttgt ccattctatc tccccaccgt
    13081 gttttgactc tccatgtgaa taaaacggtg attgataagg atacccagca taaaattctc
    13141 aagtactttg tcatctatgc tatgattttg ctatccctta tctttattgt cagcctagat
    13201 agcaatgatt ttctgatcgt gaccagcgct gtctttagct gtttcaataa tatcgggcct
    13261 attctaggaa ccacttctag tttctcaatc tttagtccta tctcaaaaat tctcctctcc
    13321 tttgcaatga ttgcaggccg cttggagatt tacccaatcc tccttctctt tatgaagaga
    13381 acttggtcta agagataaaa tacaaaatcc ctctttttac aattgtgaaa gagggatttt
    13441 tatattaaaa agggggaacg tttttaaatc caagcctgtc tgtaagcaaa gtccttactc
    13501 aaataaaaga tggaaaaact tactacacag cttggtaatg ctccaataca aattgtaagg
    13561 ctttacttgg attctttgtt aaatgaatat attccattcc tctggtagca actatctgaa
    13621 tacctaatct ttctgcatcc accttcatct ctctataata actccgaact tgtggagcca
    13681 gaataattaa atcataaaca cccataatat cataatgagc tccgtacgct cctgaattcg
    13741 caatcactct aacttctgct aattgagcgc cctcgttaat tgcattggct aattgcgcac
    13801 ttgttccaga ccctgcacaa agaaccagta ctttcaactc cttacttttt atttcatcta
    13861 actcaccagg cattatttca cttgtctcct cctctaaaat aatatcattt gagcttgcaa
    13921 catcttcttt catcagtaac tgtctatcat acgctctaca gaatggcaaa taaatcaata
    13981 tgtcgacaac taaaatcaaa gataaaaata caaaggagat agattgaaaa ttcgttccaa
    14041 ttaacaatcc caagggacca ggaaaggccc aaggtaactg gatataaaat ccattcattc
    14101 caaagaaatc aataaagacc tttcctagaa atacattcac tggtggagtc atcaaaaaag
    14161 gtacaaaaag atagggattc aaaataactg gcataccaaa tagaagaggt tcattcaccg
    14221 caaataaaac aggagtaatt gtagctttac ctaccgattt taattgttta gaccgcataa
    14281 agaaaatcaa aataaatggt acaacaaagg tagcccccgt tcctccgata gcagcgatat
    14341 aatttccgaa attaggcgtt aaagaatgat aaggaaactg cccatttgca agaagttgag
    14401 cattctcttc catattgctc agttgtaaag ctgtaacagc aggtaagaca atagatggtc
    14461 catgaattcc aacaaaccaa agcaaagcgc acataaacca aatcaacatc atagcaggat
    14521 atgattctgc ccccttaaaa ataggagtca atagttgttg aaatacttgg gcaaaaggaa
    14581 catctaaact aaaccgagat acaatatcta acaaaccact aataagtaaa acaaaagaaa
    14641 aaggaaaaat atctctaaaa gcttgtgata tagccccagg aacttcctta ggtaaatgaa
    14701 tagtaatgtc tcgcttaata cagaatcggt aaacatttac agtaataaag gcactgacaa
    14761 acgaagttat caatccttga gatcctaaat agtttgtcga caaacttgta gatcccgtct
    14821 tctcatcaac tacaagcgtt acagttaata ccagataact acatatggct gccaacatta
    14881 cagaagtatc atttattacc tttccaggag gcatttttct attaacattt ccagccaatg
    14941 actttgcaac tgtccctgaa acaataacac ccaaaactcc catggttagc atatacactt
    15001 tcctcatcca ggcaatagct ggactcggta actctactcc gaaattttta ggaatcataa
    15061 taataatcat caaaaagctt gaaaacataa ttaaaggcat agtagcaatg aatccatctt
    15121 taatagccat catataatta ttagtagaaa ttttttcaaa tttcccttgt tgcttttcaa
    15181 tggcagcgac tagcttatcc ataaaactcc tcctataaaa aattcaacct catatttaaa
    15241 acatattaaa catcattcaa atcgctaacc ttaattccgt tttagccctt ttacccaaag
    15301 agcacttttc ttaatatatc gcttctgtgt ctcataatca acaaagaaaa gaccatatcg
    15361 cttattgtag ccattcgccc aagaaaattg atcttgcaaa gaccatataa aatatccttg
    15421 aatattgact cccctatctc gagcctctaa aactttgtgt aaatgttgct caatataacg
    15481 gattctctta ctgtcatcaa taatatcatt ctcgccctct ggcttaacct ctttaagggc
    15541 tgtaccattt tcagttaaat aaatgacagg atgttgagga tattcttctt tgatacgaag
    15601 taacatatca aataagcctt gaggataaat attccaatcc caatctgttg tcggaatacc
    15661 aggctttttt acaaattcac ctagagcatt taatttaaaa gaggactgtc cttttattcc
    15721 tgttgaatta aattcttgtc tattttcacc tttgtattca cgaataaatt ctgaacgata
    15781 ataattcatc ccaaacatcg tattaagagg tgcagctctt ttcataattt ctaaatcacc
    15841 atcttcaata ataaagctag aattattagc ttccaatatt tgatttaagt gaaaaagcgt
    15901 gtcatcactg tagtagccca aaaaagttcc atctaataga aatttattat tataaacatc
    15961 ataccgttta gctgccaaaa tattttcttt ttgcccatca ataggatagc ctggctttaa
    16021 agcatgaata caacctacct ttccctcaat ccctaattga tgaaattcga ggactgcaag
    16081 agcatgcgcc aacaacatat tatgattcgc ttgaattgct tcagataatt gaaaatgatg
    16141 attcggagga aactgacctc ctatatattg acctgcagca agagacatca gttcattgat
    16201 tgtaaaccaa tgcttgactt ctgtaaattc ttggaaacaa aagcgagcat atcgtatgaa
    16261 acgatcaata ttctctctat tcaaccaatc ccctgtttct aacattttct gaggcgaatc
    16321 aaaatgatgt aaagaaacaa acggaatcac attatgtttt aagcaggact gaaagactct
    16381 atggtaatac tgaacagcca atatattcgg ctccccatct atatcaggaa atatgcgaac
    16441 ccaagaaata gataaacgca aagcctgcaa accatgttct gccgccaagg ctatatcttc
    16501 ttcgtaacga taataaaaat cactagctgg atctggtaag aacggactat tttcttgcaa
    16561 ataaacatcc cacatattta ttcctttgcc atctacccta gttgcaccct ctacttgata
    16621 agcagcggta gcagctccta ataaaaactc cttcggtaag gctttaacct gtaaattttc
    16681 catgactaat cctcattgca aaccaatatg ttcttcaatc tttcgaattc gttcatattc
    16741 atcaataaaa aactttacct gatcatacaa tagcatcgtt gtcatcaaag tatcttgacc
    16801 atgaatcatg ataaaactca cttcaaaatt atctccagat gcctcctccg ctaataaatt
    16861 agtctgttct cgatgggcag acactattga gtcgtttgca gactcaacca attctcttgc
    16921 ctgttcaaaa tatccatctc tcgcaaacgc caaagcatcc atcaaatctg accttgcatc
    16981 tcctgcgtaa gcgacaattg taaaaccaag catatttatt tcttcttttg atttcatctt
    17041 tcttcctcct caatcttgat acatctttta cacatcatta ttaaaatgac ttcgctacga
    17101 tacaaataga aagttgaccc taaatatctt aaaaatcctt aaatttcata atatttagga
    17161 tcaatcttct ctgaatacct tagttcattt tctatactta taatttataa gtggaaacca
    17221 gcgtaatttt ttgctacttc aattacacga taaacatcca aattagattg gaatagcaat
    17281 tcgtgaccac ggatataatt caaaacattt ctccattcat cttgagcttg ccttgttttt
    17341 ccagttgctt gaagatataa agccttcgcc atcttaacaa catattcacg atgataactg
    17401 agctgtttcc attcatcctt ttgactattt aataacttag aaatattttc ctcaataatt
    17461 ggtaaaaaat tatcagctag attgtaagct atataataat gattcgctaa aacatcattt
    17521 tgacggctgc caattgcatt aaaataatca catgaggaat aactagataa tttttctaaa
    17581 tattcaacaa cagactgcca atttgaccca tacaaagcag aaaagtattc ttcaatcaat
    17641 tcttcataac ttcttgtctt cttccagagc atttgcccca taacataatt aggaaaatta
    17701 tgagggaatc cggcacgtaa ttcttgacac gaaatgtacc cgttcaaatg taggtttgaa
    17761 agataagata catctttgta aatagtttga ctaattttca tatagcctaa atcgccataa
    17821 tgagcacgcc ctaaaggata gtcatatacg aaactatctc ctttaaatgt tttttgccac
    17881 tcaaaaagat aagataaatt ttcttcaaga gaattcggaa gtataatttt attacgcaga
    17941 taaggtttag gcgtaggtat ggaattgtca aaatctacat ctgcataact catttcaaat
    18001 gttcttgtaa tcggtgcaaa catcatggta aagcgttcag gattatctaa tttttctttc
    18061 tgaggcgccc ataacaactc atggtaaagt aggaaacaaa tctttgtatc taatccctca
    18121 ctcgttaaag ccctatccaa ttgattgaga atacgaatat actgatccga aaccaattct
    18181 tgtctacagt tttcgcattc acaaatatta ttacgagcat ccgacaacca tacatgtaag
    18241 tagttaacat caggtctttt cttggcataa tccttaataa tttctaccat cttatcagct
    18301 acatctggat ttgaaaaatc caggctggtt aaaatcggag ccgtattaaa caattctcgt
    18361 ttcccgttta tttcagcgac atagggtttc ttctcctctg aaatactaag acctgattcc
    18421 cagccaaatt ttgaagagta acctaaaacc tcacctgtcc atccatgacc aacacgatga
    18481 tgaataagac ctcttttttg caattcttta tccaacctat cactcaattc ttgtactaat
    18541 tcatttgaga atttttcttt atttaggtat ggattaaact catgttcata ccaacgtttc
    18601 aaaaaagaat agggattttc aaactgaatg aaaaaactgt tcatcccaat cttaggtagc
    18661 caatcaatga aatctagtat attttcaaat gaatctgctc cctctataca tactccccta
    18721 tgatagtaac ttgctgtttc atctatagat agctctttat ctaaaaaatc ttcaaatctt
    18781 aactctggaa taaagtcatg tccgcgccct ggtctagtat acacaacacc aaattcatga
    18841 aacattcggt aaattccaat aagtaaacct acaattgtat tggactcaat tctcccctta
    18901 cctttgttta atgagatgaa aatatggtct tttccatcct cttcattgac taatcctaca
    18961 aaaatatccg cttccttctc ctcgacaagt tctatatcgg tattaaatac ctgattcata
    19021 tagtatctta attcctctaa ggcaaagcta gaataagtat ttttctgttg atcaaatact
    19081 ctgataacca tcttatttcc ctccaacagt agctttttct ttttcaagat tgcgtttatc
    19141 tgcaattttg aagaatggat aataaattgc tacagaaacc aaaatcaaaa caacttgtaa
    19201 tagagctcct cgccaactag cccctgtcgc aaggaagcct cctatgatag gtggcattgt
    19261 ccacggaagg attacacctg ttgtataggg tactaagcct acagccattg atacataggt
    19321 aatcaaggca ttgattgtag gagtagcaat aaacggaatc agcataatag gatttaaaac
    19381 cgttggaaac gtaaatagaa tagctgtatt gatattaaag atagacggta taatagctag
    19441 cttacctaat gttttattag cacgactctt actaaatagg aagagacaaa tcgcaagacc
    19501 aatagtcgct ccaccgccac caataaatac aaataaatct ttaaacggta atgtaataat
    19561 gtgttgtagg gtttgtcctg cagccacagc ttcttggttt tctgtagtaa attgtaacca
    19621 aactgggtct acaaaagcat ttaaaacttg tcccccatta actccacaga accaaaagaa
    19681 tgagtttaca ataacacata aaatcatacc tggaagtgtt cctgcaatta acttaagcgg
    19741 cgttccaaca attgcaccta aaagtccgtt gagacctcct gcaactcctg ctgcttccag
    19801 accttttaag accaaagccc acaaaacaaa agtagtaaaa ccaggtaaaa gagctgaaaa
    19861 tgatttactg accacatctg ggacaccatt tggcattttt atcgtaattc cgcgttggat
    19921 aaaaatacga taaatctccg ctgtaaccaa tccaatggta atcgccataa acaaagaaga
    19981 cgcattcagg ctagaaaatg gtattgcacc tccaaataac tgcttgacct gctccccatt
    20041 tttatcataa accatacttg aaaaacgagg ggccatcaat acaaaactgg ataaggctat
    20101 gatccctgca gacggaccat ctgtaccata tccttccgac aacctgtagg caataccgaa
    20161 acaagcgacc aaggagataa tcgctactgt tgactgattc atagttgtca agatacttag
    20221 caatccaaca ctattcagcc aatttgtaaa agcctcttga ggccaactga ttagaataag
    20281 aaataaagag ccgactaaaa tcaaaggcat tgccatcata aatccttcac gaatagcttt
    20341 caaatgccgt tgattgccaa ctttttcacc aaacggagcg actttcgttt ctaaaaacgt
    20401 taaaatattg ttcatcaccg gaactccttt ttttattttt tataaattaa atataccact
    20461 aacagaaagc gttgtcaata tattttttac tgtttttaca atattttttt ctttgtatag
    20521 aataataatt ttaattgatt ttttcttatg tttaatataa aataaaccaa aggaggaaac
    20581 aaatgattac aaaacaatca gcctttttac aaaatagagc cactatactg gaatttctat
    20641 atcgaaatcc agcaacatcc cgtacagata ttgttaatga aacaggactt actccagcaa
    20701 caacgactaa catcataaaa gagctaagcg aacaatccct catatacgaa actggtgatg
    20761 aatttagcga gttttccgga tctggaagac gtagaaaaac tatttctatt acagataata
    20821 tcccatctgt tgttggaggt attgaaataa acgtcttagg tatctttctc agtctatgtg
    20881 acttacaagg gaaaactctt ttcgagacag aaattttgaa tgaagattat cctatttcag
    20941 aaatcaattc caccattacc aatatgataa aaacagctac aaagtatgtc cctttggaga
    21001 caaaattact tggatttggc ttatcaatac ctggacatta taacaaagac tccggaagca
    21061 tcattacaaa caaccccata tgggaatcat ttaatttatt aaatgtaatt aaaaaattcg
    21121 atttcccttt tattgtaaaa aataatatcg attgtatggc tataggacaa taccttttta
    21181 acccacacaa tacccccgat aactttattt tcctacacgc tggattaggt atttacactt
    21241 cctttttcac aaaagaaaaa ataggagcct ctaaaaatcc ttatatcgga gaaattggac
    21301 acaccattgt cgaattaaat gggcaatatt gtgaatgcgg aaaaaaaggt tgtttacaaa
    21361 catatatttc ggatgcttgg ttaatcaaac attcccaatt attatttaaa aattctcaac
    21421 taactgtact aaaaagcctt gtaaagactg aaaaagacat tcatttagat acccttttaa
    21481 cagcttacaa cttaggcgac tctgctttac gccaacaaat tgataaagga gtcaatttat
    21541 tggccacttc tattgcaaat cttctcctca tcaatcctgc tgataaaatc tatatcaaca
    21601 gtcaattgct taattatcaa cctttcactc atgaagtcag ggataaaatc caagatcagc
    21661 tccacttcgt tccctttact cgtaatatag aaattgaaat tttaccttac aacaaacatc
    21721 gtggaagtat aggagcttgt gcattagcta tcgttgcttt tttcatagag catagcaatg
    21781 tattacaaga tattatttca ccttaatata ttagaaatct atagacctgt ttaaatcaac
    21841 tataacctgt agtaaatatc tcgtatttag acaatatgaa aacaagacga cttccatata
    21901 ggaaaccgcc ttctcgctat gctgcatgaa actgtaaaaa tagatgaaag tgatagcggt
    21961 agtcaaatca agaggcatca taactctaaa aagtcacaat atataagttc atcctcagaa
    22021 aaatatcatt ctaattattg aaatgcctac atgaaaagaa acgttaaatg ctcataaaac
    22081 aaggaataca ggtatcaaaa ctgtgacaaa acaagtccct aaatttacta aagacactac
    22141 tcaactttac acctgtaaat ggttgttgta taataaagtt acaaagatgt acgaccacac
    22201 tgttgtaaat catagtgttc gcgaatatat tactgatagc atttctacaa atacaagtaa
    22261 agggattgga tgagattcaa acgaaatatg tcagtgtttt ggcattccta gccttcatat
    22321 catttaaaga attgtataga taaaattttt cccaatacag acactcgtaa caactgcttc
    22381 atttttctac caacatattt aggaacagga taagatacaa gaggatcaat ccataaatca
    22441 gttctatacc aatctaagac aaataagcta aaaaaacgat tgataataag caaatagatt
    22501 ccaaattttc tctatctgct cattttaata aacaatacta gtgtaactat ccttccagtc
    22561 agaagcttgt caaatcacac cgaaaattct tctaaaattt atctcgttag gcaatcaagc
    22621 aaaaactcaa cgatagtaca aacattatca tacaggattg acttcctaaa ttatatactt
    22681 tagtaaggtt tttggataag aaaaaaggtt cattttacat ttctaaacac tcttttctaa
    22741 tacgagataa ttaaaaaaac tacattttta aatataaact acaataaaat aaactaaatg
    22801 ccataggaga aagacaatgg attggtacga ttatatgata caggcatcca aacaatcaca
    22861 attcaacgca agccattggt ttcgctattt gcgaaaagtt atttttgaag actattctta
    22921 tttaacaaac caagatatag aaaagttgct agactccaaa gaactaaccc attttcaaaa
    22981 aattagcttg aagtatgcct ttcaagagca tactccaact cataaatatg tgatttcatt
    23041 aaataaacct gctaagttaa ccaatgttca aaaattgatg gagaaataca agcatggata
    23101 aaatgaaacc ggtcttccaa gcactaaata aggaattaat tcaggaaaat ctgactttaa
    23161 caattatctg tgtcggtggt tatgtcttag aatatcatgg tttgcgtgcc acacaagatg
    23221 ttgatgcttt ttatgaccaa aatcagaaaa tcaatgaaat tattgctcgt gtaggaaaac
    23281 agtttaatct taacacccac gaagagttat ggctcaacaa tcatgttgca aatatgaaca
    23341 aacaacctcc cttaagttta tgcgaatcct tgtactcatt tgagaatctg accgttttag
    23401 tagtccctat tgagtatgtg ttaggaatga agatgatgag tatcagagag caggatttga
    23461 aagatattgg tgcaattatt aaatacaaaa actttcattc accattcgat acctttaaat
    23521 atttaaaaga catgggattt gatactattg acctctctgt tctattagaa ggttttagtt
    23581 atgcttatgg tatggattgg ttagaaaaat tttttaaaga aaatcaagat aaacttcgcg
    23641 aattttatta acagtatcaa acaagatgat tgtcaattag ccagtcgtct tgtttttatg
    23701 atgaaatgaa ataaaatcgg aacaaatcac ttagagaatt ccagtcaatt tctagcactg
    23761 tttgagacgt ttcagcggac tatagcaaaa tgaaataaga ataggacaaa tcgatcagga
    23821 cagtcaaatc gatttctaag aatgttttag aagtaaaggt gtactattct actttcaatc
    23881 tattatattt cagattcggt ccactattcc ttcccaaaaa cattgcatat tgcgccgaag
    23941 gaattgctca tactatccca agcacaaaac cctcagtcgt ttgaccgagg gttccttttt
    24001 attattcaag aacttgaggc tctataatat ttgtagtggg taaatcccct atggatatta
    24061 tggagcctat ttt
//
LOCUS       DAVKMU010000029        23916 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKMU010000029 DAVKMU010000000
VERSION     DAVKMU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.029"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(86..835)
                     /locus_tag="VW343_001348"
     CDS             complement(86..835)
                     /locus_tag="VW343_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9755892.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            968..3304
                     /locus_tag="VW343_001349"
     CDS             968..3304
                     /locus_tag="VW343_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9755893.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFGVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            complement(3741..6887)
                     /locus_tag="VW343_001350"
     CDS             complement(3741..6887)
                     /locus_tag="VW343_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEU9755894.1"
                     /translation="MIRTEVTSMTVPDRLPARMRMDGRSIFQQIKNYFDTENKEYFKH
                     PNTYDGISMHLEPNILTSMEHFDLTGFHCECKDFQNQGVCKHWVAMDLYFRSLPQAIQ
                     ERIGKASHKPSFASQLIPTLPSEELQDELVEEKATAPSLALHGQVEIRNHSLAWTLKL
                     QVEQAPRAYVIKDIAHFIFLIFQKEDYFVSQKIGTIRLSLNQFNQASQNLLLYIKKYF
                     IDRNEHSYFNFSYGINPRDYGRYLETPVSYLNDLVPLFQALDVFQYVTSKAEYPLIFL
                     DDSPFIPEEEIFKVVKSNNHYEIINTAYFGFIIQEKLWIRHNHFHIIKEEHRYFLDKL
                     ATWIYHYQENSPLIFSKENKAELMQVCNIISNYVPISIPDELQIHDFIPTFAFSKTRN
                     EIALNMVWSFGEKQVHSKQDLLTLPYTYQASKARKIYHQLLSAGFKEEFHSLSKIKIV
                     DFFLKELPRFRTLGQVQLDESLEKLLVEDPAVIDIFDDESFLSVQFDFSMISEDEVEK
                     AIQALWNQESHYQTKQGKVLVFDDESLKVAQSLQDLRAKFSDGKIKMHKSRAFSLSET
                     FKDNEHVNFSRDFKKMAYDLTHPEEFDIKPYEVKAKLRSYQKEGVKWLSMLDHYHFGG
                     ILADDMGLGKTLQTITLLEANLKPDQKALILAPASLLYNWKEEFRKFVPHKQVEVAYG
                     SKTERIKQIEKSATITITSYPSFRSDLEHYQKQSYDYLILDEAQMIKNSQTKTAQALR
                     EFDVKTCYALSGTPIENRLEEIWSIFQIVLPGLLPSKKEFSKLSPQLVAKLIQPFVLR
                     RKKDEVLTELPELSEHLYSNELSSSQKTLYLAQLRRMQEMVSGASAYEIKRHKIEILA
                     GLTRLRQICNTPALFLEDYKGDSGKMDSLFELLDTIREKGSRPLIFSQFTSMLDLIEQ
                     ELEKKEMSHFKITGQTPSDKRQEMVNLFNQGEKDCFLISLKAGGTGLNLTGADTVILC
                     DLWWNPAVEMQAIGRSHRLGQTKQVDVYRLITLGTIEEKIQELQESKKELFNTVLEGQ
                     ESRSNLSVDDIKEILGVE"
     gene            complement(7076..8314)
                     /locus_tag="VW343_001351"
     CDS             complement(7076..8314)
                     /locus_tag="VW343_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755895.1"
                     /translation="MHIFLKNRAFRQLTVNEWISSFGDTIFYLAFINYVSSYAFAPLA
                     IFLISLSETIPQVLQLFTGVIADFQKNRISKYISILFIKVLLYSGVTLLLTSTDFSLF
                     SVFFICSMNLISDTIGFLAGYMLTPIYIRLINDDMTEAMGFRQSTSSIVRLIGNLSGG
                     VFLGLFSISTLAFVNVLTFLFAFLGSLLIRNRLKKEEEKIEVPPYVGMSSFFQHLKES
                     MKLLMTMEDVMVLLWILSISQAVLMMVEPVSAILLIHHPFMGLSTGQSLAILIMISLL
                     HVILGGLLSGFLSKKISIRLNIYWSLLMESLIVIDFLRGSFLLILLGSAGDAFSAGVL
                     SPRLQAMIFGIIPEELMGSVQSSINVINLLIPAVLSLALVFLATSAGLEVVAFALIIL
                     LLIAAYLVHQMKNLPNQEEV"
     gene            complement(8316..9752)
                     /locus_tag="VW343_001352"
     CDS             complement(8316..9752)
                     /locus_tag="VW343_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9755896.1"
                     /translation="MTPIKDKVRRVKTPMMVNPDTDLTISSVQQDYFSLALIGFSLLT
                     GDFLSFSKGDQKTGLSAFIKICHLIKIARLDNKITKQQEYWLYDLLMISQGEKIQKIK
                     QLKQVTSDILLNTPDFSSYFEKYNFKEEAENIKSYLLAKSMDKSGRLFPSNEFGEFVS
                     PVSFQHGFGGVLFFMNKYYVEEDENTVKEWLTKLENYEAANFLHGYSLLFGKAGFLFG
                     ILDRYEKTKERYLIDISKRLVDHLMRVYDNISNLDFALGKSGILLSLMKYCTIFDDKK
                     LANFIKNNINDAYSLLESEDNGDIYSNNFAHGRSGAAYVLKAYTDIFGDSRYQNHLQK
                     FSDGISELLEEKLSSFSKLDNLGLSWCDGVSGLILYLCLIDKERYSEIIYKSQLEMVQ
                     QYEAMGTSFCHGLSSLLQTTIYNKNQKVEQFIKKILLTRSYRNNDRLLQFQGEDGINS
                     YFDFGVGNLGIYWTLLGYTFPFELSKGD"
     gene            complement(9826..10356)
                     /locus_tag="VW343_001353"
     CDS             complement(9826..10356)
                     /locus_tag="VW343_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEU9755897.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPSWKMDLFSEEEKRIYFQTTCEVS
                     SKDSAINKYKMEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSYIHKLGI"
     gene            complement(10350..10818)
                     /locus_tag="VW343_001354"
                     /pseudo
     CDS             complement(10350..10818)
                     /locus_tag="VW343_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11367..12044
                     /locus_tag="VW343_001355"
     CDS             11367..12044
                     /locus_tag="VW343_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9755898.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLCKEVSPVFVQKWQKEAVKLEFALGQIPDKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            12031..12828
                     /locus_tag="VW343_001356"
     CDS             12031..12828
                     /locus_tag="VW343_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9755899.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLEIEETTYLQETGPERGIGRIGNIQPQTFGELAQHVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            12845..13948
                     /locus_tag="VW343_001357"
     CDS             12845..13948
                     /locus_tag="VW343_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9755900.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            14249..15268
                     /gene="galE"
                     /locus_tag="VW343_001358"
     CDS             14249..15268
                     /gene="galE"
                     /locus_tag="VW343_001358"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9755901.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            15270..16205
                     /locus_tag="VW343_001359"
     CDS             15270..16205
                     /locus_tag="VW343_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9755902.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTNWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSYHRS"
     gene            16380..17024
                     /locus_tag="VW343_001360"
     CDS             16380..17024
                     /locus_tag="VW343_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755903.1"
                     /translation="MLSKEEYIEEIGLIEKQNYVEVELYPLVADIINPTLKNSLSKRY
                     VFGRRKSNMGQIYYGLSNFPDIVILDKNYQNKARKSIEIEEWKKLRGCVEIKSLKHDL
                     ITEEKIKSTISNSFEHITGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKEEIDNTIV
                     QVVNKRIETEEAGNSFDWWKNIKDLSFNYTDIYLSKDCIQEWDEFVKKVKEIEW"
     gene            17063..17164
                     /locus_tag="VW343_001361"
                     /pseudo
     CDS             17063..17164
                     /locus_tag="VW343_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(17215..17421)
                     /locus_tag="VW343_001362"
     CDS             complement(17215..17421)
                     /locus_tag="VW343_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9755904.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDILEAVKNCPTRALIGNQEA"
     gene            17473..17949
                     /locus_tag="VW343_001363"
     CDS             17473..17949
                     /locus_tag="VW343_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9755905.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKGFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMVTGSWFANPGDVIKIK"
     gene            17958..18629
                     /locus_tag="VW343_001364"
     CDS             17958..18629
                     /locus_tag="VW343_001364"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9755906.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            18760..19098
                     /locus_tag="VW343_001365"
     CDS             18760..19098
                     /locus_tag="VW343_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9755907.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            19101..19730
                     /locus_tag="VW343_001366"
     CDS             19101..19730
                     /locus_tag="VW343_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9755908.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            19970..21121
                     /locus_tag="VW343_001367"
     CDS             19970..21121
                     /locus_tag="VW343_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9755909.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            21434..22183
                     /gene="truA"
                     /locus_tag="VW343_001368"
     CDS             21434..22183
                     /gene="truA"
                     /locus_tag="VW343_001368"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9755910.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            22173..22946
                     /locus_tag="VW343_001369"
     CDS             22173..22946
                     /locus_tag="VW343_001369"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9755911.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            22924..23385
                     /locus_tag="VW343_001370"
     CDS             22924..23385
                     /locus_tag="VW343_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9755912.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     AIIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            23612..>23916
                     /locus_tag="VW343_001371"
     CDS             23612..>23916
                     /locus_tag="VW343_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9755913.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDR
                     YGIEFVKKGKNRYYSPDLKQEMINKVLHEGWTKDRVSLEYGLPSRTILLNWLAQYRK"
BASE COUNT         7069 a         4344 c         5084 g         7419 t
ORIGIN      
        1 tgttttagaa gtagaggtgt actattctag ttccaatcta ctatataaat gaaaacctta
       61 gctgtttggc caaggttcat ttcttctatg caagttcttc tcttttctta tctgggttcc
      121 agatgaagct tgcgataaag ctaaagatga tggttaggat agcgaggata atagcaagga
      181 tgagggcagg gatgcggata aaccaccaga gtggatttgg ttttttatca ttgtgccatt
      241 gtttcgtttc ttcgtccaga cgttggaatt ctgtttgaat acgattggcg actgtttcaa
      301 tgccttcatc attcattttc ttgatatctg agatatcgat tggatttcca aagttcatat
      361 cgacacgttc acggctaatc aagcccttca aagtcatggg accggtgtag gtaaccggca
      421 tgatacggac cttggccatt ttggcaatca gtgctgcgcc ccccttgaca tcgtttgagt
      481 ggcggctacc acttggaaac atgatgagag agcggtcact ttttttgaga acgttgatag
      541 gatatttgat ggctgaggcg ctgggatttt cacggtcgat gggaaaggcg ccacacatac
      601 gaatccacca accaaagata cggttggtaa agagttcttt ttttgccata aagatgaact
      661 gttttggctt ggtcgcaaag gccatataaa caggatccca ccaggtacgg tgaggcgcaa
      721 ctaaaatata attttcatct tgattaggaa ttttatcagt attatgatag tgagcattgc
      781 cattgatgga ccataagagc aatacaacta atccacgcaa ataagtataa aacatgcgat
      841 ctccttcgat tgttttcttg ttattattat accttatcaa aggagggctg gcaaactttt
      901 cccttgacta gatgcatatt tgggatgaaa ttagaattct gttaaaaaaa atgatataat
      961 agaatttatg gataaaaata agattatggg attaacccaa agagaagtca aggaaagaca
     1021 ggctgagggt ttggtcaatg actttaccgc atcagccagt accagcactt ggcaaatcgt
     1081 taaacgaaat gtctttaccc tttttaacgc tttgaacttt gccattgctt tggcccttgc
     1141 ctttgtgcag gcttggagca atctggtctt ctttgctgtt atctgcttta acgctttttc
     1201 tgggattgtg accgagctac gagccaaaca catggtggac aagctcaatc tcatgaccaa
     1261 ggaaaaggtc aaaaccatcc gtgatggtca ggaagttgct cttaatcctg aagaattagt
     1321 gctaggagat gtcattcgtt tgtctgcagg agagcagatt cctagtgatg ccttggtttt
     1381 ggaaggcttt gcggaagtca atgaagccat gttaacggga gaaagtgatt tggtgcaaaa
     1441 ggaagttgac ggcttacttt tgtcaggaag tttcctagcc agtgggtcag ttttatctca
     1501 agttcaccat gtcggtgcag acaactatgc tgccaaactc atgcttgagg ctaagaccgt
     1561 taaacccatc aactcccgta tcatgaaatc gctggacaag ttggctggtt ttactgggaa
     1621 gattatcatt ccctttggtc tggctctctt gctggaagcc ttgcttttaa aaggcctgcc
     1681 tctcaagtca tctgttgtaa actcgtcgac agctcttttg ggaatgttgc ctaagggaat
     1741 tgcccttttg accattactt cgctcttgac tgcagtgatt aagctaggtt tgaaaaaggt
     1801 cttggtgcag gagatgtact ctgttgagac cttggcgcgc gtggatatgc tctgtctgga
     1861 caagacgggc accatcaccc aaggaaagat gcaggtggag gctgttcttc cactgacgga
     1921 aacttacggt gaagaggcta ttgccagcat cttgactagc tacatggccc atagtgagga
     1981 taagaatcca actgcccaag ccattcgcca gcgttttgtg ggagatgttg cttatcctat
     2041 gatttccaat cttcccttct cgagcgaccg caagtggggg gctatggagt tagaaggctt
     2101 ggggacagtt ttcttagggg cacctgagat gttgctggat tctgaagtcc cagaagccag
     2161 ggaggccttg gagagaggat cacgtgtctt ggtcttagct ctcagtcagg agaaattaga
     2221 ccatcacaaa ccacagaaac catctgatat tcaggctcta gccttgctgg aaatcttgga
     2281 ccccattcga gagggagcag cagagacgct ggactatctc cgttctcagg aggtgggact
     2341 caagattatc tctggtgaca atccagttac ggtgtccagc attgcccaga aggctggttt
     2401 tgcggactat cacagctatg tagattgctc aaaaatcacc gatgaggaat tgatggccat
     2461 ggcggaggag acagctattt tcggacgtgt ttcccctcat caaaagaaac tcatcatcca
     2521 aacgttgaaa aaagcgggtc atacaacggc tatgacaggg gacggggtta atgatatctt
     2581 ggcccttcgt gaggcggatt gttctatcgt gatggcggag ggggatccag caacccgtca
     2641 gattgccaat ctggttctct tgaactcaga ctttaatgat gttcctgaga ttctcttcga
     2701 gggtcgtcgc gtggtcaata acattgccca catcgccccg attttcttga taaagaccat
     2761 ctattccttc ctgttagcag tcatctgtat tgccagtgct ttactaggtc ggtcagagtg
     2821 gattttgatt tttcccttca ttccgatcca gattaccatg attgaccagt ttgtggaagg
     2881 tttcccacca ttcgttctga cttttgagcg aaatatcaaa cctgtcgagc caaacttcct
     2941 cagaagatcc atgcttcgtg ccctaccaag tgccctcatg gtcgtgtttg gcgttctttt
     3001 tgtgaaaatc tttggaacga gtcaaggttg gtctgagtta gaaatctcaa ctctcctcta
     3061 ttatctcttg gggtcaattg gtttcttatc cgtatttaga gcctgcatgc cattcactct
     3121 atggcgagtc ctattgattg tctggtcagt aggaggcttc ctagccacag ctctcttccc
     3181 aagaattcaa aaactgcttg aaattgcaac cttaacagga caaacattac ctgtttatgg
     3241 tgtcatgatg ttggtcttta cagtgatttt catcctgact agtcgctatc aagctagaaa
     3301 ataaagaaag actgcaatct gtggattgca gttttttata cgatggatct atcgtagatc
     3361 tgatgtgcaa ggcctacgtg tctattatct atcggtgaac ccaagagtga ttctcaagcc
     3421 tgcttggatt gaggtaatag attcaaatat ctgtagttag actatttgaa gtctgatgca
     3481 agaaataatt ctcttcgaaa atatcttcaa accaggtccg ttctatccac aatctcaaag
     3541 cggtactttg agcaacttgt ggtcagctcc ctagtttgat agtataaaaa ctgtcagata
     3601 aaggggtggc gaaattgtat ctaaacgaac agaaacgtga tggtaaggcg tatagagctc
     3661 ataggatggc aaggaaagtc tagaacctaa aaaagaacta gataaatcca ctaagtgtgg
     3721 ttactctagt tctttttcgt ttattcaaca cccaagattt ctttaatatc atcaacgctc
     3781 agattgcttc tgctttcttg tccttcaagg acggtgttga agagttcctt tttactttct
     3841 tggagttctt gtattttttc ctctatggta ccaagtgtga taaggcgata gacatcgact
     3901 tgtttggtct ggccaaggcg gtggcttcgg ccgatggctt gcatctcaac tgctggattc
     3961 caccagaggt cacagaggat gacggtatca gctcctgtta gattcagtcc tgttccacct
     4021 gcttttaggg aaatgaggaa gcagtctttt tccccttgat tgaaaagatt gaccatttct
     4081 tgacgtttat ctgaaggtgt ttgtcctgtt attttaaagt gagacatttc ttttttctct
     4141 agttcttgct caatgagatc aagcatagag gtgaattgtg aaaagataag tggtcgtgac
     4201 cctttttcac gaatggtgtc taggagctcg aataagctgt ccatttttcc actatcccct
     4261 ttgtagtctt ctaaaaagag agcaggggta ttacagattt gtctaagtcg ggtcagtcct
     4321 gctaagattt caattttatg acgtttaatc tcataggcgc tggctcctga aaccatttct
     4381 tgcatgcgtc gaagttgagc tagatagagc gttttttgcg aactgctgag ttcattgctg
     4441 tagaggtgct cgctcagttc aggcaattct gtcagaactt cgtctttttt cctacgcaga
     4501 acaaaaggtt gaatcagttt agcaacgagt tgaggtgaca attttgaaaa ttccttttta
     4561 cttggtagga gacctggtaa tactatctgg aaaatagacc aaatctcttc gagacggttt
     4621 tcaataggag ttccagatag ggcatagcag gttttgacat caaattctct taaagcttgt
     4681 gctgttttgg tctgactatt ttttatcatc tgtgcttcat ctaggatgag gtagtcgtaa
     4741 gactgctttt gataatgttc aaggtcagat cgaaaagatg gatagcttgt aatagtgata
     4801 gtagcagatt tttcaatttg tttgatacgc tcagttttgc taccataggc tacttcaacc
     4861 tgtttgtgag gcacaaattt tcggaattct tccttccaat tataaagaag gctggctgga
     4921 gctaggataa gagctttctg gtctggttta aggttggctt ccagaagagt gatggtttgc
     4981 aaggttttcc ctagacccat gtcgtctgcc aggattcccc caaaatgata atgatccagc
     5041 atactcagcc acttgactcc ttctttttga taggagcgga gtttggcctt gacttcataa
     5101 ggcttgatat caaattcttc agggtgggtc aggtcataag ccatcttttt gaaatcacga
     5161 gaaaaattga catgttcatt gtctttgaag gtttcagaaa ggctaaaggc acgagatttg
     5221 tgcatcttga tttttccgtc tgaaaattta gcgcgaagat cttggagact ttgagcaacc
     5281 ttgagacttt catcgtcaaa gactagaacc tttccttgct tggtttggta gtggctttct
     5341 tgattccata aagcttggat agctttttca acctcatctt ctgaaatcat agaaaaatca
     5401 aactgaactg aaaggaagct ttcgtcgtca aaaatgtcaa tgacagcagg atcttctact
     5461 aagagttttt caagcgattc atctagttga acttgaccca gagtacgaaa acgggggagt
     5521 tcttttagga agaaatcaac gattttgatt ttggaaagac tatgaaattc ctctttgaaa
     5581 ccagcagaaa gcagttgatg atagattttt ctagctttgc tagcttggta agtatagggg
     5641 agggttagga gatcttgttt agaatggact tgtttttctc caaaggacca caccatatta
     5701 agggcaattt catttctagt cttggagaag gcaaaggtgg ggataaagtc atggatttgt
     5761 aattcatctg ggatactgat aggaacatag tttgagatga tattacagac ttgcatgagt
     5821 tcagccttat tttcttttga aaagataaga ggagaatttt cttgataatg atagatccaa
     5881 gtagctaatt tatccaaaaa gtaacggtgt tcttctttga tgatgtggaa gtgattgtgt
     5941 cgaatccaaa gtttttcttg aataatgaaa ccaaaataag ctgtattgat gatttcataa
     6001 tggttattag atttgacaac tttaaaaatt tcctcttccg ggataaaggg actatcatct
     6061 aagaatataa gagggtattc ggcttttgag gttacatatt ggaagacatc caaagcttga
     6121 aaaagtggga cgaggtcgtt taaatagcta acaggagtct ctaaatagcg tccgtaatct
     6181 ctagggttaa tgccataact gaagttgaaa taagaatgtt catttctatc gataaaatat
     6241 tttttaatat agaggagtag gttttgggat gcttgattaa actgattcaa ggataatctg
     6301 attgtcccga ttttctgtga aacaaagtaa tcttcctttt ggaaaatgag aaaaatgaag
     6361 tgagctatat ccttgataac ataggcacga ggagcttgtt ctacctgtaa ttttaaggtc
     6421 caggctaggc tatgatttcg gatttcgact tgtccatgaa gggcaagtga aggcgccgta
     6481 gctttttctt ctaccagttc atcttggagc tcttcagatg gcaaggtagg aatcagttgg
     6541 ctggcaaaac ttggtttatg actcgcttta ccaatgcgtt cttgaatagc ttgggggaga
     6601 ctacgaaaat aaagatccat ggcaacccag tgtttacaga ctccctgatt ttggaaatcc
     6661 ttacattcgc aatgaaagcc agtcaaatcg aagtgctcca tactggtcag gatgttgggc
     6721 tccagatgca tggaaatacc atcataggta ttggggtgct taaaatattc cttattttca
     6781 gtatcaaaat agttttttat ctgttggaaa atagagcggc catccattct catacgagca
     6841 ggtagtctat cgggaaccgt cattgaagta acctctgttc taatcattct tttctctatc
     6901 ttatcagaat ttataggaaa agaatagatt tatcttacat aaattgcatc ttgataggga
     6961 ttggaaacat ttgtagttgc aagatagttg tatgtaatat cgacaatcaa ttacaaggag
     7021 attacgattc catagaaact atcaagtgaa aactgactta tccataacat tctagctaaa
     7081 cttcttcttg gtttggtaag tttttcatct ggtgaactag ataggctgct ataagtagta
     7141 aaattataag agcaaaagca acaacttcca gaccagcact tgttgcaaga aatacgagag
     7201 caagggatag gacagctggg ataagtaagt tgataacatt gatggatgat tgaactgatc
     7261 ccattaactc ctcaggaatg attccaaaaa tcatggcttg caagcgagga ctcaatactc
     7321 ctgccgagaa agcatcaccc gcacttccta gtaaaataag taaaaaactt ccacgtagaa
     7381 aatctataac aatgagagac tccatcaata gactccaata aatattcagg cgaatagaaa
     7441 tttttttaga tagaaatcca ctcagaagtc cacctaaaat aacatgaaga aggcttatca
     7501 ttattaaaat tgccagactt tgccctgttg acaaacccat aaaaggatgg tgaatcagta
     7561 gaatcgcact gacaggctct accatcatca aaacagcttg actaatggac aaaatccaaa
     7621 gcaagaccat gacatcttcc atagtcatca ataatttcat tgactctttc aaatgctgga
     7681 aaaagctact cattcctaca taaggaggaa cttcgatttt ttcttcctct tttttcaagc
     7741 ggtttcggat aagtagactt cctagaaagg caaataaaaa ggttaaaaca tttacaaaag
     7801 ctagagtaga aatgctaaac agtcctaaaa atactcctcc gctgagatta cctatcaaac
     7861 gtacaatgga actcgtggac tgcctaaaac ccatagcctc cgtcatatca tcgtttatta
     7921 gtcgtatgta aatcggtgtc agcatgtatc ctgcaagaaa accaatcgta tctgaaatca
     7981 agttcatgct gcaaataaag aaaacagaaa agagagagaa gtcggtactt gtcaggagca
     8041 aagtcactcc agaataaagg aggaccttga taaagaggat agaaatatac ttgctgattc
     8101 gatttttttg aaaatcagct ataactccag taaaaagttg caagacctgt ggaatggttt
     8161 ctgataggct aatcaaaaat atagctaagg gagcaaaggc ataagaagag acataattga
     8221 taaaagctaa gtaaaaaata gtatctccaa aacttgaaat ccactcattt actgttagct
     8281 gacgaaaagc tcgattttta agaaaaatat gcatattaat ctcctttact taattcaaat
     8341 gggaatgtat aaccgagtaa agtccaataa atacccagat ttcccacacc aaagtcaaaa
     8401 taactgttta ttccatcttc cccttgaaat tgtaataatc tatcattatt acgataagaa
     8461 cgagtcaata agattttttt tatgaactgt tcaacctttt gatttttatt gtatattgtt
     8521 gtttgcaata aactacttaa accatggcaa aaactagttc ccattgcttc atattgttgc
     8581 accatttcaa gttgtgattt atagattatc tcagagtatc tttctttatc aatcaaacaa
     8641 agatacaata ttaatcctga aactccgtca caccaagaaa gacctaaatt atctaattta
     8701 gaaaagcttg aaagtttctc ttctagtaat tcagatatcc cgtcagaaaa tttttgtaaa
     8761 tgattttgat agcgactatc tccaaaaata tcagtatagg cttttaatac ataggcagca
     8821 cctgaacgtc cgtgagcaaa attattagaa tatatatctc cattatcctc ggattctaat
     8881 aaactatatg catcatttat attgttcttg ataaaatttg ctagtttttt atcatcaaaa
     8941 atggtacaat atttcatcaa actgagtagg ataccagact tccctaaagc aaaatctaag
     9001 ttactaatat tgtcataaac ccgcattaaa tgatcgacta atctcttact tatgtctatt
     9061 aaatatctct cctttgtttt ttcataccta tctaggattc caaataggaa acctgctttc
     9121 ccaaatagta gagagtaacc atgtaaaaaa ttagctgctt cataattttc taatttcgta
     9181 agccattctt taacggtatt ttcatcttcc tcaacataat atttattcat aaaaaataaa
     9241 actcctccaa aaccatgttg gaaagaaaca ggacttacga actcaccaaa ttcatttgac
     9301 ggaaacaaac gaccagattt gtccatagat ttagccagta agtatgattt tatattctca
     9361 gcctcctctt taaaattata tttttcaaaa taacttgaaa aatctggagt atttaaaaga
     9421 atgtcacttg taacttgttt caattgcttt attttttgga ttttttctcc ctggcttatc
     9481 attaataaat cgtatagcca gtattcttgt tgtttagtta ttttattgtc taatctagct
     9541 atttttatta gatgacatat ttttataaag gccgataatc ctgttttttg atcacctttt
     9601 gaaaaggata aaaaatcacc tgttaataaa gaaaatccaa ttagtgctaa actaaaataa
     9661 tcttgttgaa ctgagctgat agttaggtca gtatctggat taaccatcat tggtgtttta
     9721 actcgtctta ccttatcctt gataggagtc attgtctcca agtctattaa tgttatatca
     9781 ccttttttgg tatatataaa atttgtagga gctatatcta caattttata ttcccagttt
     9841 atggatgtaa cttactatat tcacaatgtt atccagtgtt ttttctctaa tatttaagga
     9901 gtgttctgtt tctcgaataa attcttcaaa gtttaacccg tcaacttgtt cctgaacaag
     9961 aaaataatca tccacgatat aaaattcatc agttaaatta gtagtataac ttttatcggc
    10021 taattttttt agcatgtgag cttcattttt tatatcatca agagctgtcc attctccttc
    10081 agcatcataa ttcacaaaag gtcttgactg cttgatgatt actttttgcc cgtccgattt
    10141 tctaattgcc cgataaacat ttcctttatt tgatctctta ataatttttt ccattttgta
    10201 tttatttatt gcagagtcct tacttgaaac ttcacatgtg gtttgaaaat aaatcctttt
    10261 ttcttcttct gaaaataaat ccattttcca gctaggaaga ctagggaaat tttgtctctt
    10321 atcttctaca tagttcttcc ttttttcatc tagcaataaa tagatgactt ttttattttt
    10381 ttcatcataa gcttgttttt ttaaaaaagc cccatatcta taatgaactg gagaatgcat
    10441 tccacattga tagtcagata gtatttttgg agccttaaat tctgacagtc tattcgtaag
    10501 attacaaatc atactcttag cttcagattc tgacttagga tatagagtta taaatttgtt
    10561 agcagtaggg ctcatttccc taggggaatt aatttttttt aattcctcta aatttttaac
    10621 aactttaaag ctacaattat ttagttggga tagtttatac acaatcttaa aaatatttac
    10681 agcgtctttt atttgggagc ttatgtgtat tttccaccct tgtgcaggca tcttatctga
    10741 taaataatag taattccaaa tatcaccctg ttggaaattt ttatcacatt ttaaacattc
    10801 tttctctaat aaatccataa caaactccta attattaaaa taggctagag acacagcact
    10861 agcctattcc gtaagtaaag ctacttattt tatataaaat ataactagcg acctgtaatt
    10921 aaacaagtaa aagaacaata cttagggaga cctgtgacaa tgatatcttc ctctggaagt
    10981 agttctaatt caaacaatgt ttgttccatg actgtatcct cctgttatga taaattccag
    11041 aaagcataga gcaaattcgt ggttcgtctg actaaatttc ttatctgaac accaccttca
    11101 aatacaaatt ttagtcaaac ttttattttt tctaactttc taattatatt ctaccatgtg
    11161 tttttatatg tcaagaattt tctgaataaa ctgtttattt tttatttttt gacatttttg
    11221 caaattgttt aaaatctttc aattggattc acttattact ttaggacatt gaaacttctt
    11281 tcgccagagg tgcttatatt actgggaagt tgaagattga aatgtggtat aataagaggt
    11341 aatagagttt tggaaagtga gagaagatga tttcaaagag attagaattg gtagcttcct
    11401 ttgtgtcaca gggggctatt ttactagatg tgggaagtga ccatgcttat ctgcctatcg
    11461 agttggttga gagaggccaa atcaaaagcg ctattgcagg tgaggtggtg gaaggtccct
    11521 atcagtctgc ggttaaaaat gttgaggctc acggcctaaa ggagaaaatc caagtccgtt
    11581 tagccaatgg cttggcagct tttgaagaga ctgaccaagt gtctgtcatt accattgctg
    11641 gcatgggtgg tcgtttgatt gctaggattt tagaagaagg tttggggaag ttagctaatg
    11701 tagagcgttt gatcctccag cccaataatc gtgaagacga cttgcgtatc tggctacagg
    11761 atcatggatt ccagattgta gcagaaagca tcttagaaga agctggaaag ttttatgaga
    11821 ttttggtggt ggaagcagga caaatgaagc tatcagccag tgatgttcgc tttggaccgt
    11881 tcttgtgcaa agaggtcagt ccagtctttg tccaaaaatg gcaaaaagaa gctgttaagc
    11941 tagagtttgc cctcggacaa atcccagaca aaaatctgga agaacgtcaa gttctagtag
    12001 ataagattca agccatcaag gaggtgctcc atgttagcaa gtgaagtgat ccaagcttat
    12061 gaagcctttt gccctcagga attttctatg gagggagaca gccgtggtct gcaaattggc
    12121 actttagaca agggtatcca aagggtcatg gttgccctcg atattcgtga agagacagtg
    12181 gcagaggcca ttgaaaaggg tgtggacttg attatcgtca agcacgcgcc tatctttcgt
    12241 cctatcaagg acttgcttgc cagccgtccg caaaatcaga tttacatcga cctgattaag
    12301 catgacatcg cagtttatgt cagccatacc aatattgata tcgttgaaaa tgggctcaat
    12361 gactggtttt gtcagatgct agaaatcgag gagacgactt atctgcagga aacaggtcca
    12421 gaacgtggaa ttggacgtat tgggaatatt cagcctcaga cctttggaga attggcccaa
    12481 catgtcaagc aagtctttga cctagatagc cttcgaatgg tgcattatca agagaatgat
    12541 ttgcagaagc ctatttcaag agtagcaatt tgtggtggaa gcgggcagtc tttctataag
    12601 gatgctttgg caaagggggc agatgtctat atcactggtg atatctacta ccatactgct
    12661 caggatatgc tgtctgatgg cttgttagca ttggatccag gtcactatat cgaagtgctt
    12721 tttgtggaaa aaatcgcagc actccttact caatggaaag agaaaaaagg ctgggaacta
    12781 gagattttac ctagtcaagc atcgaccaat cctttccgtc atatctagtt agaaagtgaa
    12841 gacaatgaaa aaagttgcca ttattggagc aggaattgtg ggagcaacag ctgcctacta
    12901 cctctcgaga gaaagtgacc tagaggtgac cgtttttgac catgagcaag gtcaagccac
    12961 caaggccgca gcaggaatta tcagtccttg gttttccaaa cgccgtaata aagcctggta
    13021 caagatggcg cgcttggggg ctgattttta tgtggattta ttagctgatt tagagaaatc
    13081 aggacaagaa atcgactttt accagcgttc gggagtcttt ctcttgaaaa aggatgaatc
    13141 caatttggaa gaactttatc aactggccct ccagcgcaga gaagaatctc ccttgatagg
    13201 gcaattagcc attctgaacc aagcctcagc taatgaatta ttccctggtt tgcagggatt
    13261 tgaccgcctg ctctatgctt ctggtggagc gagagtagat ggccaactct tggtaactcg
    13321 tctgctagaa gccagtcatg tcaagctggt caaagaaaaa gtgactctga caccgttagc
    13381 atcaggctac cagattggtg aagaggagtt tgagcaggtt attttggcga cgggagcttg
    13441 gttgggggac atgttagaac ccttgggtta tgaagtagat gttcgtcctc aaaaaggaca
    13501 actacgagat tatcagctgg tccaagatat ggaagcttac cctgttgtca tgccagaagg
    13561 ggagtgggat ttgattcctt ttgcaggtgg gaaattgtcc ttaggcgcta cccacgaaaa
    13621 tgacatggga tttgatttga cggtagatga aaccttgctc caacaaatgg aggaggccac
    13681 cttgactcac tatctgattt tggctgaagc tacttcaaaa tctgagcgtg ttggaatccg
    13741 tgcctacacc agtgatttct ctcctttctt tgggcaggtg cctgacttaa ctggtgtcta
    13801 tgcagccagt ggattaggtt catcaggcct cacaactggt cctattattg gttaccattt
    13861 agctcaactg atccaagaca aggagttgac cttggaccct gaaaactacc caattgaaaa
    13921 ctatgtcaaa cgagtaaaaa gcgaataata ctcaatgaaa atcaaagaac aaactaggta
    13981 actagccgcg ggttactcaa agcactgctt tgaggttgtg gatagaactg acgaagtcag
    14041 ctcaaaacac tgttttgagg ttgtagatag aactgacgaa gtcagtaacc atacccatgg
    14101 caaggcgacg ttgacgtggt ttgaattgga ttttcgaaga gtataagcat tttactgaaa
    14161 ttttatcagg tagtttagga tggcaaatga cattccctgt caaaaatggt aaaataagaa
    14221 aaaataatct gagaatcgag gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc
    14281 cggttttatt ggaacccaca ctgttattga attgatccaa gcaggccatc aagttgttgt
    14341 ggtggataat cttgtcaata gcaatcgtaa gagtttagaa gttgttgaaa gaattacagg
    14401 agttgagatt cctttctatg aggcagatat tcgtgacaca gctaccctca gagatatttt
    14461 caagcaggaa gaaccgactg gtgtcattca ctttgctggt ttgaaggctg ttggcgaatc
    14521 aacacgtatc cctcttgcct actatgacaa caatatcgct ggaactgtca gccttttgaa
    14581 agccatggaa gaaaacaact gtaaaaacat catcttcagt tcttctgcga cagtttacgg
    14641 ggatccgcac acagtgccca tcttggaaga tttcccactt tcagtgacca acccatacgg
    14701 tcgtaccaag ctcatgctag aggaaatttt gaccgatatt tacaaagcag actcagaatg
    14761 gaatgttgtc ttgcttcgtt actttaaccc aatcggagcc catgagagtg gtgatttggg
    14821 agaaaatcca aacggtattc caaacaatct cttgccatat gtgactcaag tagccgttgg
    14881 aaaattagag caagtgcaag tgtttggaga cgattacgat acggaagatg gaacaggtgt
    14941 tcgtgactat atccacgttg tcgatttggc taagggtcac gttgcagctt tgaaaaaaat
    15001 ccaaaaaggt tcaggactaa acgtttataa ccttggaact ggtaaaggtt actcagttct
    15061 tgaaattatc caaaacatgg aaaaagcggt gggacgtcct attccttacc gtatcgtaga
    15121 acgtcgccca ggtgatatcg ctgcctgcta ctcagaccca gcaaaagcta aagcagaact
    15181 cggttgggaa gcagaactcg acatcaccca aatgtgtgaa gacgcatggc gttggcaaag
    15241 caagcatcca aatggatttg aagactaaga tgatgatttc aatcatcgtc ccttgtttaa
    15301 acgaagagga agtacttcct cttttttatc aggctctgga agctttactt ccagatttgg
    15361 aaacagaaat cgagtatgtc tttgtcgatg atggatcaag tgatgggacc ttggaactct
    15421 taaaggccta tcgggagcaa aatccggcag tccattatat ttctttctct cgaaattttg
    15481 gcaaagaagc agccctttat gcaggcttgc aatatgcgac aggagatttg gtggtggtga
    15541 tggatgcaga cctccaagat cctcctagta tgttgtttga gatgaaaaat gtactagaca
    15601 aaaatgtaga cttggactgc gttgggacac ggagaactag tcgggaggga gaacccttct
    15661 ttcgcagttt ctgtgctgtt ctcttttatc gcctcatgca aaaaatcagc ccagtagctc
    15721 tgccgtcggg tgtccgtgat tttcgtatga tgagaaggtc tgtagtcgat gccattttaa
    15781 gcttgaccga gtccaatcgt ttttcaaagg ggctcttcgc ctgggtgggt ttcaaaacct
    15841 actatttgga ctatccaaat gtcgaaaggc aggctggcaa gacaaattgg agttttaggc
    15901 aactcttttt ctactccatt gaagggatta ttaatttttc agattttccc ttgattatag
    15961 cctttgtagc tggtctccta tcttgttttc tttctctgct gatgaccttt tttgttgtgg
    16021 ttcggaccct cattttgggc aatccgacat ctggttggac ctctctgatg gctgttattc
    16081 tctttcttgg aggtattcaa ctcttgacca ttgggattct cggcaagtat atcagtaaga
    16141 tttatttaga aaccaaaaaa agaccgcttt atcttatcaa agaaaaaagt tatcatagat
    16201 catgaaaaag taggagttat ctagcgcagt cacttttttg taattataat tcttttggtc
    16261 tggaacttga gtgattttta aaaaaattag ggatttgaca tgatataaat gaactatgtg
    16321 gaataaattt actgcaaaga cgataactta ataaataaaa aattcaggag ggttatttta
    16381 tgctaagtaa agaggagtat attgaagaaa tcgggcttat agagaaacaa aattatgtag
    16441 aggttgaact gtatccttta gttgctgata taattaatcc gactttaaaa aatagtttat
    16501 caaaaagata tgtcttcgga agaagaaaga gtaatatggg gcaaatatat tatggtctaa
    16561 gtaattttcc tgatatagtt attttggata agaattatca aaataaagcc agaaaaagta
    16621 tagaaattga agaatggaag aaacttcgag gatgcgtgga aattaagagt ttaaagcatg
    16681 atttaattac agaggaaaaa atcaaatcaa ccatatcgaa cagttttgaa catataactg
    16741 gagagatggg acaacttatt ggagaccttt tatggtataa aaaagtaatt tatacaaatg
    16801 gaattgaatg gagatttcta agtttagatg acaaggaaga gatagataat acaattgttc
    16861 aagtagttaa caaaagaata gaaacagaag aagcgggaaa ttcttttgat tggtggaaga
    16921 atattaaaga tttatcgttc aattatacgg atatatattt atctaaagat tgtatacaag
    16981 agtgggatga atttgtaaag aaagtaaagg aaatagaatg gtaggagtct attctcattg
    17041 agaaatttag attataagtc gcagtaagat ttatttagag actaaaaaaa gaccacttta
    17101 tcttatcaaa gaaaaaagtg accttcctga ttttacagaa aaaaataaag tgaaaagact
    17161 ataattttac atggaaatgt gctaaactag agggagtgga ttatcgccat tgatttaggc
    17221 ttcctggttt ccaatcaggg cgcgagttgg gcaattttta acagcctcta agatatcctg
    17281 actaggagaa atttcttttt ccagttggtc agggtcatcg taaaaacgca cgattccatt
    17341 atcgtggtaa tcaaataaat cagaataagt ttggcaaagc ccacaggcga tacatcgttc
    17401 aggtataagt gtgattttca tgttcatatt tatattgtaa taagaaaagt aaaaaaaaac
    17461 aaggagtaag gtatggcaaa agaaccgtgg caagaagata tctatgatca agaagaatca
    17521 agagcagagc gtcggcatcg aaaccacgga ggggctgata ggatggctaa tcgtattttg
    17581 acgatcctag ctagtatttt ctttgtaatt gtggtggtga tggtcatcgt tctcatctat
    17641 ctatcatcgg gggggagtaa tcgcacagca gccttaaaag gctttcatga ttctgatgca
    17701 agtgtagtac aaatctcatc ttcaagcagt tctcagcctg agcagagttc agagccagaa
    17761 tctacttcta gtagttcaga agaagctgct aatcctgaag gaacgattaa agttctcgca
    17821 ggagaagggg aagcagctat tgccgctcgt gcaggaatct ccattgctca gttagaggcc
    17881 ttgaatcctg ggcacatggt tacaggatct tggtttgcta atccaggtga tgttataaaa
    17941 ataaaatagg agccgatatg aaaacaattc aaattgctat tgatggtcct gcttccagcg
    18001 gtaagagtac ggtcgcaaag attattgcta aggattttgg attcacctac cttgatacag
    18061 gagctatgta tcgtgcagcg acctatatgg ctcttaagaa ccaattagga gttgaagaag
    18121 tcgaagccct tctagccttg ttggaccagc atccaatcag ctttggacgt tcagaaactg
    18181 gagaccagct tgtttttgta ggagatgtgg atattaccca tcctatccgt gaaaatgaag
    18241 tgaccaatca tgtttctgct attgcagcaa ttcctgaagt gcgtgagaaa ctggtttctc
    18301 tccaacaaga aattgcccag caaggcggga ttgtcatgga tggtcgcgat attggaactg
    18361 ttgtattgcc acaagcagaa ttgaaaattt tcctagtagc ttctgttgat gagagagcag
    18421 agcgtcgtta caaggaaaat attgccaagg gaattgaaac agaccttgaa accctaaaaa
    18481 aggaaattgc tgcgcgtgac tacaaggata gtcatcgtga gacttctcct ctcaaacaag
    18541 cagaggatgc tgtctacctt gatacaactg gtttgaacat tcaagaagta gttgaaaaaa
    18601 tcaaagcaga agctgaaaaa agaatgtaaa tgtagaaaag ccgatagatt gatatcggtt
    18661 tttttctaaa tttgtcaaaa tgctgatttt agggtataat agttgctgtt cgagaaattt
    18721 tgattttcaa agaatagtga atgatgataa ggagaaacca tgaacaattt accaaattgt
    18781 ccaaaatgta actcagagta tgtctacgaa gacggtgccc tactggtttg cccagagtgt
    18841 gctcatgagt ggaatcctgc tgaagttgca gaagtagaag aaggtcttgt cgctatcgat
    18901 gccaacggaa ataaattgac tgatggtgat acagtaactc tcatcaagga cttgaaagta
    18961 aaaggtgcgc caaaagattt gaaacaaggg acgcgcgtga aaaatatccg catcgtagaa
    19021 ggcgaccaca atatcgactg taagattgat ggttttggtg ccatgaaact taaatcagag
    19081 tttgtgagga agatttaagc atgtcaaaac actataaact tgtattttat agccgtatct
    19141 tcttgtttct agcggctttt acgggagttt atcttgaaat tactaagcat ggtggttttg
    19201 ggatgcttct ctattacacg gttctgtcca acctcttggt aactattttt accctttatc
    19261 ttctaaaggt tatgagccgt gtaggtgaaa attggcaaag gccaagtctc ttgcgcttaa
    19321 aaggtggggt caccatgagt atcatgatta cctgtgtgat ttaccatttc ctcttggcgc
    19381 ccattgcgac taatttctat accctagaaa atttcctttg ccactatatc gttcccatct
    19441 ggtttttagc ggataccctc ttttttgaca aacagggtca atacaagatt tgggatccag
    19501 cagtgtggac cattttaccc tttctgtata tgatgtttgc tctttttaat ggcttggttc
    19561 taaaactcaa tattccaaat gctaaggata atcctttccc ttacttcttt ttgaatgtga
    19621 acaagggttg gaatgttgtg tttaagtggt gtctgattat ctttgttgcc tatatggtag
    19681 caggatttat tttctacttt atcaagcaaa tcaagagaaa gtcatcctaa tactcttcga
    19741 aaatctcttc aaaccacgtc agcttccatc tgcaacctca aaacactgtt ttgaacaacc
    19801 tgcggctagc ttcctagttt gctctttgat tttcattgag tataagatac aagggattca
    19861 ttttcgaatt taagaaggag tctacaaacc aatgaggctt cttcttttct tgcttatttg
    19921 ataactatca aaaaagtaga agattaagag aaatatagaa agagatttca tgaaacaatt
    19981 tttagaacgg gccagcattt tggctctctc cctcgttttg attacctcct tttccatttc
    20041 gagtgcccta ccagccatgt ttgactatta tcagggttat tctaaggaac aaattgagct
    20101 cttggtgagc ttgccttcct ttggaatcat gatgatgtta ctgctaaatg gtttcttaga
    20161 aaaaatattt cctgagcgct tacagattag tttgggcttg ctgattttat cattgagcgg
    20221 tacagctccc ttctggtacc aagcctatcc ctttgtcttt ggaacacggc ttctctttgg
    20281 tttgggtctt gggatgatca atgccaaggc catttctatt atcagtgaac gctaccaagg
    20341 aaaaaggcga attcagatgt tagggctacg cgcttctgca gaggtcgttg gagcttctct
    20401 cattaccttg gccgtcggtc agttgttggc ctttggttgg acagctatct ttctagccta
    20461 tagtgctgga tttttggtgc tgccccttta tctgctcttt gtcccttatg gaaaatcaaa
    20521 gaaagaagtc aagaaaagag cgaaggaagc aagtcgttta actcgagaaa tgaaaggctt
    20581 gatttttacc ttagctatcg aagcggcagt tgtagtttgt accaatacag ctattaccat
    20641 ccgtattcca agtttgatgg tggaaagagg attgggggat gcccagttat ctagttttgt
    20701 tcttagtatc atgcagttga tcgggattgt ggctggggtg agtttttctt tcttgatttc
    20761 tatctttaaa gagaaactgc tcctctggtc tggtattacc tttggcttgg ggcaaatcgt
    20821 gattgccttg tcttcatcct tgtgggtggt agtagcagga agtgttctgg ctggatttgc
    20881 ctatagtgta gtcttgacga cggtctttca acttgtctct gaacgaattc cagctaaact
    20941 cctcaatcaa gcaacttcat ttgctgtatt aggctgtagt ttcggagcct ttacgacccc
    21001 atttgttcta ggtgcaattg gcttactaac tcacaatggg atgttggtct ttagtatctt
    21061 aggaggttgg ttgattgtaa tctctatctt tgtcatgtac ctacttcaga agagagctta
    21121 ggattgattc ctaagttttt cttttgatac tcaatgaaaa tcaaagagca tagttgattg
    21181 agtttggaat agtacgctgt agctgctaaa atatttctag aaattaattt gacttttcta
    21241 atagagttat tcatatctta tttcaatcta ctatagaaag ctagccgcag gttgctcaaa
    21301 atagtgtttt gaggttgcag atggaaactg acgtggtttg aagagatttt cgaagagtat
    21361 gagattttta tacccataca atatttgttt taggtccttg tttacagact aatttcttga
    21421 taaaatagag gttatgacga gatataaagc aactatttcc tatgatggtt atgcctttgc
    21481 tggctttcag cgccagtctc atgcgcgtag cgttcaggaa gaaattgaaa aaaccttgac
    21541 cagattaaat aaagggcaaa ccattactgt tcacggtgct ggtaggacag atagtggggt
    21601 tcatgccctg ggacaggtca ttcattttga cctgccttat cagatggatg aggagaaact
    21661 ccgttttgcc ttggataccc agtctcctga agatattgat gtgatttcga ttgagcttgt
    21721 ggcagatgat tttcattgcc gttatgccaa acatagcaag acctatgagt ttattgtgga
    21781 tagaggacgt cccaaaaatc ctatgcgccg tcactatgcc acccactttc cctacccact
    21841 cgatgtggaa cgaatgcaga ttgcaatcaa aaagctagag ggaacccatg attttaccgg
    21901 ttttacagcc tctgggacta gtgtagagga taaggttcgc accatcacag aagctagttt
    21961 aatagtcgat gagacaggac aatttttgac ctttaccttt tcaggaaatg gtttcttgta
    22021 taaacagatt cgcaatatgg tggggactct tctcaaaatc ggtaacaacc gcatgccagt
    22081 agagcagatt gatctcatct tggagaagaa ggacaggcaa cttgcaggtc ccactgcagc
    22141 accaaatggt ttgtatttaa aggagattcg ttatgaagaa taatcgtatt ttagcacttt
    22201 ctggaaatga tatttttagt ggtggtggac tgtcagctga tttggctacc tataccttga
    22261 acggcttgca tgggtttgta gcagtgactt gtttgacagc cttgacagaa aaaggatttg
    22321 aagtctttcc aactgatgat accatttttc aacatgaatt agatagcttg cgtgatgtgg
    22381 aatttggggg aattaagatt ggtcttctcc ctactgtcag tgtggctgag aaggccttgg
    22441 actttatcaa acaacgccca ggagtacctg tggtgttgga tcctgtcttg gtctgcaagg
    22501 aaacgcatga tgtagctgtc agtgagctct gccaagagtt gattcgcttt ttcccttatg
    22561 tcagtgtgat tacgcctaat ctcccagaag cagaattatt atccggtcag gaaattaaaa
    22621 ccttggaaga catgaaaact gcagcgcaga aattgcatga tttaggagct ccagcagtca
    22681 ttatcaaggg aggcaatcgt cttagtcagg acaaggcggt ggatgtcttt tatgatggac
    22741 agacctttac tatcctagaa aatccagtta tccaaggcca aaatgctggt gcaggttgta
    22801 cctttgcctc tagcattgcc agtcacttga ttaaaggtga taaacttttg ccagcagtag
    22861 aaagctctaa ggctttcgtt tatcgtgcta ttgcacaagc agatcagtat ggagtaagac
    22921 aatatgaagc aaacaaaaac aactaaaatc gcccttgtat ccctattaac cgccctttct
    22981 gtggttctag gttatttctt aaaaatccca acacctacag gaattctaac tcttttagat
    23041 gctggtgtct tctttgcggc cttttacttt ggtagtcgtg aaggagcggt agtcggagga
    23101 ctagcaagtt tcttgattga cctcttatca ggctaccctc agtggatgtt ctttagcttg
    23161 gtcaaccatg gcttgcaggg atttttcgca ggatttaaag gaaaaagtca gtggttaggc
    23221 cttattttag caattattgc catggtagga ggctacgcct tgggttctac tttgatgaat
    23281 ggctgggcag cagccctccc agaaattcta ccaaatttca tgcaaaatat ggtagggatg
    23341 attgtaggat ttattcttag tcaaagtatc aagaagatta agtaatactc ttcgaaaatc
    23401 tcttcaaacc acgtcagctt ccatctgcaa cctcaaacca ctgttttgag cagcctgagg
    23461 ctagctttct agtttgcact ttgattttca ttgagtataa agaggttgag taaaaagtat
    23521 ttcaaataag aaaaacgcat agtatcaggt gttgaatgaa ctgcccccca aaagttagac
    23581 agaaaaaatc taacttttgg ggtgttttat tatgaaacta acttatgatg ataaagttca
    23641 gatctatgaa cttagaaaac aaggatatag cttagagaag ctttcaaata aatttgggat
    23701 aaacaattct aatcttaggt atatgattaa attgattgat cgttacggaa tagagttcgt
    23761 caaaaaagga aaaaatcgtt actattctcc tgatttaaaa caagaaatga ttaataaagt
    23821 cttacatgaa ggctggacta aagatagagt ttctcttgaa tacggtctcc caagtcgtac
    23881 gatacttctt aactggctag cacaatacag gaaaaa
//
LOCUS       DAVKMU010000030        23217 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKMU010000030 DAVKMU010000000
VERSION     DAVKMU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.030"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            261..1742
                     /locus_tag="VW343_001372"
     CDS             261..1742
                     /locus_tag="VW343_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9755914.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            2104..3105
                     /locus_tag="VW343_001373"
     CDS             2104..3105
                     /locus_tag="VW343_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9755915.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3256..4173
                     /locus_tag="VW343_001374"
     CDS             3256..4173
                     /locus_tag="VW343_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9755916.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4184..5074
                     /locus_tag="VW343_001375"
     CDS             4184..5074
                     /locus_tag="VW343_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9755917.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            5103..6719
                     /locus_tag="VW343_001376"
     CDS             5103..6719
                     /locus_tag="VW343_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9755918.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6729..8048
                     /locus_tag="VW343_001377"
     CDS             6729..8048
                     /locus_tag="VW343_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9755919.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8258..8330)
                     /locus_tag="VW343_001378"
     tRNA            complement(8258..8330)
                     /locus_tag="VW343_001378"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8295..8297),aa:Lys,seq:ctt)
     gene            complement(8595..9866)
                     /locus_tag="VW343_001379"
     CDS             complement(8595..9866)
                     /locus_tag="VW343_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9755920.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            10136..10336
                     /locus_tag="VW343_001380"
     CDS             10136..10336
                     /locus_tag="VW343_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755921.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            10540..10719
                     /locus_tag="VW343_001381"
     CDS             10540..10719
                     /locus_tag="VW343_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755922.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10856..11035
                     /locus_tag="VW343_001382"
     CDS             10856..11035
                     /locus_tag="VW343_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9755923.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            11072..11524
                     /locus_tag="VW343_001383"
     CDS             11072..11524
                     /locus_tag="VW343_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9755924.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11815..12285
                     /locus_tag="VW343_001384"
     CDS             11815..12285
                     /locus_tag="VW343_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9755925.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12304..13392
                     /locus_tag="VW343_001385"
     CDS             12304..13392
                     /locus_tag="VW343_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9755926.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            13373..13531
                     /locus_tag="VW343_001386"
     CDS             13373..13531
                     /locus_tag="VW343_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEU9755927.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            13641..14675
                     /locus_tag="VW343_001387"
     CDS             13641..14675
                     /locus_tag="VW343_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9755928.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            14737..16920
                     /locus_tag="VW343_001388"
     CDS             14737..16920
                     /locus_tag="VW343_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU9755929.1"
                     /translation="MGPITIPKKTIITLEHLKYLHFSLEETKTYWEKNNIIDGILISI
                     YYNRIVAKSNRINGYFNVGGGNPFPNDTIVGAKFNDEKTKHIVTHYISRDALNKTITV
                     LSKIIEVFEEHFDRAITCEMFSDSSTFASINFSEYGISKSKFQQYLRDSCFIENFGVE
                     HTTVSDIQNSIVTFYDVHTDIFRLLNKLNIDISEANIMNQTTVLLDEKNIELLLSKAP
                     YLVSMIVEDFSKLSVDDFSLDNNDLKINLPSPMNEPVVGVIDTLFDKRVYFNEWVEYH
                     DFVSPDISKDSQDYKHGTAVTSLIVDGANLNPNLDDGCGNFRVRHFGVSLQSGFNSFT
                     IIKQIKEIVSQNADIKVWNLSLGSNDEIRENFISAEGALLDEIQFENDVIFIIAGTNA
                     SVINGKRKRIGAPADSLNSIIVNSVDFNNQSVSYSREGIVLSFFVKPDVSYYGGGNGD
                     FINVCEPLGLGRVAGTSFAAPFIARKMAYLIHIMGLSREEAKALLIDAAIPWNDKKTF
                     TDLSLIGNGIVPIKMDDILSTPDDEIKFIVSDISRAYDTYNYDFPVPISSESYPYVAK
                     ATMCYFPNCSRKQGVDYTNTEMQLTFGRLKSDGIKSINKDNQHAEDTPGYVRENAARN
                     IFRKWDNVKHIGESFTSRKRAKAILNPSNPQWGMSIKTIERLKSGDGQGVRFGVVVTL
                     KELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSLNEEIEFE"
     gene            16971..17987
                     /locus_tag="VW343_001389"
     CDS             16971..17987
                     /locus_tag="VW343_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9755930.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYRDFYEFQNKVYISRTENEEKYSN
                     FIYESLFSDKVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDDMRTSLPVINQPAIDKKYNENPHQDDIVNIVTLNYT
                     HVIDKLYNGSAKSFRNQLRANLYNFYIEPPIHAHGTVDVCTVLGVSDEIQISNSLEEQ
                     KESLIKSLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDRYIWSQIAERSISGSV
                     PVIIYHYVPHFDPGNPIRAKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIFD
                     LIER"
     gene            18002..18952
                     /locus_tag="VW343_001390"
     CDS             18002..18952
                     /locus_tag="VW343_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9755931.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTVLA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            18954..19697
                     /locus_tag="VW343_001391"
     CDS             18954..19697
                     /locus_tag="VW343_001391"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9755932.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            19708..21504
                     /gene="pepF"
                     /locus_tag="VW343_001392"
     CDS             19708..21504
                     /gene="pepF"
                     /locus_tag="VW343_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9755933.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            21751..22305
                     /locus_tag="VW343_001393"
     CDS             21751..22305
                     /locus_tag="VW343_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9755934.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLSNQGL
                     HYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPDFPKNRVHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            22416..23084
                     /locus_tag="VW343_001394"
     CDS             22416..23084
                     /locus_tag="VW343_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9755935.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         7374 a         3807 c         4899 g         7137 t
ORIGIN      
        1 ttttaaaaaa tcaaattctt taattgttgc tattatataa tttttaaaat taaaaatata
       61 atctttagac ttgaatttga ttctgtattc acgccctcaa tagacggtaa aataagaaaa
      121 ttgtttatat attgtctccg tagtgttatt atacgaaata aaagatttta ggaaaagtcg
      181 ttatatcatg ctatacctat tctttgtggt ataattgcaa gaggtttaat ccgataattt
      241 ataaagagag aaagatattc atgagagatt tattatctaa aaaaagtcat aggcaattag
      301 aattattaga attattattt gaacataaac gttggtttca tcgttctgaa ctagcagagt
      361 tattaaattg tacagaacgt gcagtcaaag atgatctatc ccatgttaaa tctgcttttc
      421 ctgacttgat ttttcattct tctactaatg gtatacgcat tattaatacc gatgatagtg
      481 atattgaaat ggtttaccat catttcttta aacattcaac tcatttttcg attttagaat
      541 tcatcttctt taatgaaggt tgtcaagctg agagtatttg taaagaattt tatatcagtt
      601 catcttcgct ctatcgtatt attagccaaa tcaataaagt gattaaaagg caatttcaat
      661 ttgaagtcag tctgacccct gttcaaatca ttggaaatga gagagacatt cgttactttt
      721 ttgcacaata tttttcagaa aaatattatt tcctagaatg gccatttgaa aatttttcat
      781 cagagccact atctcaattg ttagaattgg tttataagga aacaagcttt ccaatgaatt
      841 tgtcaacgca tagaatgcta aagttgctcc tagttacgaa cctatataga ataaagtttg
      901 gtcattttat ggaagtagat aaagattctt ttaacgacca aagtttggat tttttaatgc
      961 aggcagaagg aatagaaggt gttgctcaga gttttgaatc agaatacaat atctctttag
     1021 atgaagaagt tgtttgccaa ttatttgtgt cttactttca aaaaatgttt ttcatagatg
     1081 aaagtctctt tatgaaatgc gtaaaaaagg atagttatgt tgaaaaatct taccatctat
     1141 tgagtgattt tattgatcag atttcagtca agtatcagat tgaaatggag aataaggata
     1201 atctgatttg gcatctgcat aataccgcac atctgtatcg tcaagagttg tttactgagt
     1261 ttattttatt tgatcaaaaa gggaatacaa tcaggaactt tcaaaatatt tttccaaaat
     1321 ttgtttcaga tataaaaaaa gagctttctc actatttaga gactcttgag gtttgttcta
     1381 gttcaatgat ggtaaatcat ctatcttata ccttcatcac tcataccaaa catttggtga
     1441 ttaatttgct acaaaatcag cctaaactaa aggttttagt tatgagtaat tttgatcagt
     1501 atcatgcaaa attcgttgca gagacacttt cttattactg tagcaataat tttgaactgg
     1561 aagtttggac tgaattagaa ttatcaaagg aatctttaga agattcacct tatgatatca
     1621 ttatttctaa ttttattatt cctcctattg aaaataagag actcatctat tcaaataata
     1681 taaacacggt ctcactcata tacttgttaa atgccatgat gtttattcga ttagatgagt
     1741 aaccaaaaat aacaaaaaaa cgctatttcg cgtttgaact gtacctcaaa agttagacag
     1801 aaaaaatcta atttttgggg tgtttttgtt aagaaaagac cttcaatcat ttaagcaaaa
     1861 tgtacatcta gatttatagc cctctaaaac tcttgctttt tttgtagaag atacgaaaaa
     1921 agccataaaa ccaaggcttt tcctgtttta tgtagatgtt tacacatgaa aattgaagta
     1981 ttttcaaaag ctaattggag tgattttatt catgatggga taaatgatct ggccaaaaat
     2041 gattgagaaa tagttgctta aaaagcattt gcatgatact atatattgaa aggaggtgat
     2101 aatatggtag aacaaagaaa atcaattacc atgaaagatg ttgctttaga agcaggagtt
     2161 agtgttggaa ctgtttcacg tgtaattaat aaagaaaaag gcattaaaga agtaactttg
     2221 aaaaaagtgg aacaagcgat taaaactttg aattacattc cagattacta tgctagagga
     2281 atgaaaaaaa atcgaacaga aacgattgca atcattgtac caagtatctg gcatcccttc
     2341 ttttcagaat ttgctatgca tgtggaaaat gaagtctata agagaaataa caaattactc
     2401 ttatgttcta taaatggtac aaatagagag caagactatc tggagatgtt gcgtcataat
     2461 aaagttgatg gagtggttgc cattacctat agtccaattg aacattactt gacgtcagga
     2521 attccctttg ttagtattga ccgcacatac tcagatattg ccattccttg tgtttcatca
     2581 gataatgatg caggtggaag agaagcagct aaacaattaa ttagtaaagg atgtcagcat
     2641 ttagcctttg tgggcggaca taatacaacc attaatgaaa ccaaacgtcg tagaatttca
     2701 tttgaaaagt atgtccaaga gcataaaata cctcttagta tttttgattt ggatgagaca
     2761 gttactgact attatggaaa gctagagagt ttcttggaag aaaattcttc tatagatgga
     2821 atttttacaa tcaacgattt tacagctttg gatgtgattg aaatactaga aaaaaatggc
     2881 aaacgtattc ctcatgatgt ccaaattatt ggatacgatg gaattaaaat ggctggagat
     2941 agagattatc tactttcaac tattgaacaa ccattggaag aaatggcaaa agaagcggtt
     3001 cgtattttgt ttgatataat tgatggaaag actgttaatt tgcagacaat cctaccagta
     3061 aaatttgttg aaggaaaaac aacaaaaaat gaaaataagt cttgacagaa agcgctatca
     3121 atgatagaat gaattcagat aaaaagattt atttttaaaa caaaaatgaa acgtttcaaa
     3181 aaaagaaata aaaagacagc gccaagcgct atcttttata gaaaaaatga aacgtttcaa
     3241 aaaaaggagg ttgctatgaa tagcaaagcg aagcaagttt ctctttggga aagaatcaag
     3301 aaacaaaaac tcttgttatt gatgactgtc cccggtttag ttttaacctt tatctttaaa
     3361 tacatcccta tgtatggggt tttaatcgca tttaaagatt acaatccttt aaaaggaatt
     3421 ttagggagtg attggattgg tttttctgag tttacaaaat tcatatcctc tcccaacttt
     3481 ggtatcttgt tagccaacac attaaaatta agtatctatg gtttattgct tggcttttta
     3541 ccaccaatca ttcttgcgat tatgctcaat caactcttga gtgaaaaagt caaaaaacga
     3601 attcagctca ttttatacgc accaaacttt atctcagtcg ttgttattgt cggtatgatt
     3661 ttcctcttct tttcagtggg aggaccaatc aacaattttc tttctatgtt tggaatgaag
     3721 gctgacttct tgacaaatcc agacttcttt agacccttat acatctttag tggtatctgg
     3781 caaggaatgg gctgggcttc aacgctctac acggcaacat tggtaaatgt agatccagcc
     3841 ttagtagaag cagctcgact ggatggagcc aatatcttcc aacgaatctg gcacattgat
     3901 attccagctc ttaagcctat tatggttatc caatttgttt tagctgcagg tggaattatg
     3961 aatgtcggat atgaaaaagc attcttgatg cagacatcgt taaatttgcc aacttctgaa
     4021 attatctcga catatgtcta taaagttggt cttgtatcag gagactattc ttactcaaca
     4081 gcggttggtt tgtttaatgc agtgattaac gtagtattgc ttgttgcagt taaccaaatc
     4141 gttaaacgca tgaataatgg tgaaggaatt taaggaggaa agtatgaaaa attcgattat
     4201 ggatacaaaa tttgatagac gtatcttact cttaaataaa atcattattg tctttatcgt
     4261 tttgatgact ttgcttcctt tactttatat cgtcgtagca tcctttatgg atcctaaggt
     4321 tctggttagt agagggatta gctttaatcc agccgattgg actgtagaag gttaccagcg
     4381 tgtattcagt gaccaatcta ttctaagagg ttttatcaat tctctactat actcttttgg
     4441 atttgcagct ttaacagtat tgctatctgt gtttacagct tatcctcttt ctaagaaaga
     4501 cttggttgga cgtcgttgga ttaactactt cttgattgtg actatgttct ttggtggtgg
     4561 tttagtccca acttacttgc tcgtaaaaga attgggaatg ctcaatactc catgggctat
     4621 cattgttcca ggtgctgtca atgtttggaa tattattctt gctagggcct atttccaagg
     4681 attgcctgaa gaattagttg aagctgctgt cattgatgga gcgaatgact tgcagatttt
     4741 ctttaaaatc atgcttcctc ttgcaaaacc aattatgttt gttctcttcc tctatgcttt
     4801 tgtaggacag tggaactcat actttgatgc aatgatttat atcaaggatc caaacttgga
     4861 accattgcaa cttgtacttc gtaaaattct cattcagagc caaccaggtc aagacatgat
     4921 tggagcacaa gcggctatga atgaaatgaa acgtttagct gaattgatta aatacgcaac
     4981 tattgtcatt tccagcttgc cattgattgt tatgtatcca ttcttccaaa aatactttga
     5041 taaaggaatt atggctggtt cacttaaagg ataaaaaaag aaaaaataaa aggagttttc
     5101 tcatgaaatt caaaacattc tcaaaatcag cagttttgtt gacagctagt ttagcagtac
     5161 ttgcagcctg tggctcaaaa aatacagctt caagtccaga ttataagttg gaaggtgtaa
     5221 cattcccgct tcaagaaaag aaaacattga agtttatgac agccagttca ccgttatctc
     5281 ctaaagaccc aaatgaaaag ttaattttgc aacgtttgga gaaggaaact ggcgttcata
     5341 ttgactggac caactaccaa tccgactttg cagaaaaacg taacttggat atttctagtg
     5401 gtgatttacc agatgctatc cacaacgacg gagcttcaga tgtggacttg atgaactggg
     5461 ctaaaaaagg tgttattatt ccagttgaag atttgattga taaatacatg ccaaatctta
     5521 agaaaatttt ggatgagaaa ccagagtaca aggccttgat gacagcacct gatgggcaca
     5581 tttactcatt cccatggatt gaagagcttg gagatggtaa agagtctatt cacagcgtca
     5641 acgatatggc ttggattaac aaagattggc ttaagaaact tggtcttgaa atgccaaaaa
     5701 ctactgatga tttgattaaa gtcctagaag ctttcaaaaa cggggatcca aatggaaatg
     5761 gagaggctga tgaaattcca ttttcattta ttagtggtaa cggaaacgaa gattttaaat
     5821 tcctatttgc tgcatttggt ataggggata acgatgatca tttagtagta ggaaatgatg
     5881 gcaaagttga cttcacagca gataacgata actataaaga aggtgtcaaa tttatccgtc
     5941 aattgcaaga aaaaggcctg attgataaag aagctttcga acatgattgg aatagttaca
     6001 ttgctaaagg tcatgatcag aaatttggtg tttactttac atgggataag aataatgtta
     6061 ctggaagtaa cgaaagttat gatgttttac cagtacttgc tggaccaagt ggtcaaaaac
     6121 acgtagctcg tacaaacggt atgggatttg cacgtgacaa gatggttatt accagtgtaa
     6181 acaaaaacct agaattgaca gctaaatgga ttgatgcaca atacgctcca ctccaatctg
     6241 tgcaaaataa ctggggaact tacggagatg acaaacaaca aaacatcttt gaattggatc
     6301 aagcgtcaaa tagtctaaaa cacttaccac taaacggaac tgcaccagca gaacttcgtc
     6361 aaaagactga agtaggagga ccactagcta tcctagattc atactatggt aaagtaacaa
     6421 ccatgcctga tgatgccaaa tggcgtttgg atcttatcaa agaatattat gttccttaca
     6481 tgagcaatga caataactat ccaagagtct ttatgacaca ggaagatttg gacaaaattg
     6541 cccatatcga agcagatatg aatgactata tctaccgtaa acgtgctgaa tggattgtaa
     6601 atggcaatat tgatactgag tgggatgatt acaagaaaga acttgaaaaa tacggacttt
     6661 ctgattacct cgctattaaa caaaaatact acgaccaata ccaagcaaac aaaaactaga
     6721 ggttgattat gggagataag aaatacacag tagaaaaagc caatcgtttt atagcagaaa
     6781 ataaacatct cgttaatact caatataagc ctgaagaaca tttttcagct gagattggtt
     6841 ggatcaatga tccaaatgga tttgtctatt ttcgtggaga ataccatctc ttttatcaat
     6901 tctatccata tgatagtgtt tgggggccta tgcactgggg acatgctaaa agtaaggact
     6961 tggtgacttg ggagcacttg ccagtggcac ttgctcctga ccaagattat gaccgaaatg
     7021 gttgtttctc aggctctgcc attgtcaagg atgatcgcct ctggctcatg tacactggac
     7081 atatcgaaga agaaaccggt gtccgccaag tgcaaaatat ggcattttca gatgacggga
     7141 ttcactttga aaagatttcc caaaatccag ttgcaactgg atcagacttg ccagatgagt
     7201 tgattgctgc tgatttccgt gatccaaaac tctttgaaaa agatggacgc tattactccg
     7261 tagtagctgc caaacataag gataatgtgg gctgtatcgt tctactaggg tccgataacc
     7321 tagtagaatg gcagttcgaa tccatctttt taaaaggggt agaacaccaa ggctttatgt
     7381 gggaatgccc agattacttc gagttagatg ggaaagattg ccttattatg tcacccatgc
     7441 gttatcagcg tgagggagac tcatatcata acatcaactc atcgcttttg ttcacgggta
     7501 aggtagattg gggagaaaaa tgttttatcc cagaatcagt tcaagaaatt gatcatggcc
     7561 aagacttcta tgcgcctcaa acattgttgg acgatcaaaa tcgtcgtatc ctgattgctt
     7621 ggatgcagac atgggggcgt acccttccaa cccatgacca agaacacaaa tgggcatgtg
     7681 ccatgacttt acctagaatt ttaagattgg aagatggcaa actaagacaa ttccctgtta
     7741 aaaaaggcca atatcaaatc caaatagata aagattgtca ttaccactta ggaaatgata
     7801 tagattatct tgaatttggt tatgatagta atgcgcagca agtttacatt gatcgtagcc
     7861 atcttattca aaaaattcta ggtgaagaag aacaggacac tagtcgacgg tatgtagata
     7921 ttgaagctaa agaattggaa gttgttctag ataaaaattc catcgagatt tttgtcaatc
     7981 aaggtgaagc aagcttgact gcaacttatt acttaacggt gccagctgag ctatcacgaa
     8041 ttgattaaaa attaagttat ttctcctaaa gaaaaagttc tctttctaaa atagtggaaa
     8101 gaggactttt tgtgttttgg gtatataagc ttagtttatg gtatttgtaa aattggtgtt
     8161 ggattatgat ttaagctagt tttctaaaga atttgaaaaa aattttattt aagcaaaaaa
     8221 aacccttggt tccaaggctt ttcctgttgt atttatatgc cccctacagg gatcgaacct
     8281 gtgacccacg gattaagagt ccgctgctct gccagctgag ctaaggaggc aaagaaaaaa
     8341 gctgtattgg tgccgaaact tcacggtttg tattgaaccc gcgcaattaa gcaggtgggc
     8401 aactcgctct aactgaagct gtttccgtgt gagacggcct acatgctgtt agaagacttt
     8461 tgtttcccta ataatacaaa aaatagtcgg tcaacactta agtgtgaagt cgtacaccac
     8521 agcgtttcta tacttatatg ataccacttt ttcaaaaaaa atcaagagga aagtgcaatt
     8581 ttttgaaaag gatttcagat ttttcgcaaa cggtcgatag cttcctttct ttgagccaaa
     8641 gcccgttcat atttaccagt atcttctgct tggaaatagt gatgattacg aattttttct
     8701 ggcagatagt cttgcttgac ccaatttcca ggatagttgt gtggatagag atagtcttgg
     8761 gcattcccca gttccttgct tccactgtag tgcccatcac gtaggtgtcg cggaataggc
     8821 aagtgccctg atgttttgag gtcagcaagt gccttatcca tagctacata ggctgagttg
     8881 gattttggag aaagggccaa atcaatcacg acattggcaa tgagaatgcg ggcttctggg
     8941 aacccaatct tctgggcagc atccagagca gtcacggtat gaatctgggc ctcaggattg
     9001 gctaagccga tatcttcata ggcgataaca gtcaagcgac gagcgagact aggcagatcc
     9061 ccagcctcaa tcaagcgggc agtatagtgg agactggcat ccacatctga gccacgaata
     9121 gacttttgca gggcagatag aacatcatag tgaccgtctc catccttgtc catagtgatg
     9181 tagcttctct gaagactatt ttccatgatg tctaaggtga tgtggcgaat gccctcgtca
     9241 ttctcagggg tagagagaac agctaagtcc agtgagttaa aggcagagcg aaggtctccg
     9301 tttgtagagg ttgcgatgaa atccagcgca tcctcatcta gttctattgg aaaatcaaaa
     9361 ccacgttcag ggttacttag agctatctgc agggcctctt tgacgtcttg gttagacaga
     9421 ggttccaact cgaaaatttg aactcggcta cgaatggcag gagtgacaga gaagaaagga
     9481 ttttcagtcg ttgctccaat catgatgacc agtccacttt ccaagagagg caagaggaag
     9541 tcttgcttgg tcttatctag tcgatgaatt tcgtctagca ataggacgag accaccagaa
     9601 aatttagctt cttccgagat ttcttgcagt cgctttttac tatcaactgt cgcattgaaa
     9661 gttcgaaagg catacttggt cgttccagcg atggcagagg caatactggt tttgccgatt
     9721 ccaggagggc catatagaat catggaggac aggcggttgg cttccaccat gcggcggatg
     9781 atttttccag gtccgaccag atgctcctga ccgatgacct ggtcgatggt tttagggcgc
     9841 atgcgaagcg cgagattgtc tggcatagca gtcctttcta gcgtggattt gagtagaatg
     9901 actcatctac tatatcttac tatgtccatt ctagcctatt agttgtgata tttcaatggt
     9961 tttgtaataa tcagggagtg agatagaatc ttttttacta tttaatttaa tgaggagagg
    10021 tatcagcatg agagatttga ttttccttaa agctcaaaat aggatagttg agcctaaaga
    10081 agtggaagtg ctgacttgag aaataatatt gctgaatgtc attgtatgaa ctataatgaa
    10141 aagagaggtg ataagtaatg gaaatgatgg accaagtcag gaaattttga tttttacaaa
    10201 acaaattcgt cattggattc ttagtgatca agttatttca gggaaaagaa aacttttctt
    10261 ccgggaagat actccaaaag agattttaga tttgtacgaa aacattaaat ctaaacttga
    10321 ttttgcttac caataagttt attctaataa tgggttgaaa aaatatgaaa aatagaataa
    10381 ttgatgtttt tgaagtagta aatcgcatcc ttgtaataac tgttgaaaat cctgatttcg
    10441 aagacttgag agttaatcat actcaatgaa aatcaaagag caaactagga aactagccgc
    10501 aggctgtact tgagtacggt aaggcgacgt tgacgtggtt tgaatttgat tttcgaagag
    10561 tatcagtttg taaaaattgg agataaaaaa tatagagtac gcagtgttcc tatgattcat
    10621 tcaactccgc ctcaatctgt cttagatcga gatactttta caattgacta tacagatgat
    10681 gaattgctcg ataaagaagc agtttttact actcattaga aaaataggtc tgaataaatt
    10741 ggcttccctt agaaccacat ctaggggctt ttatgtataa tagaataata agtatcactc
    10801 ctttacaata cgtgtaacac gtgttataat ggtgttgtca ggaggtaaaa gcgctatgcc
    10861 tatgacacaa aaagagatgg ttaaactcct tacggcccat ggttggataa agactagagg
    10921 cggtaaaggc tcccatatta aaatggagaa gcaaggggaa agacttatca caatccctca
    10981 tggtgagctg aataagtaca ctgaaagagg gataagaaag caagctgggt tgtaaaattc
    11041 agcccctgtt ttttgatatg gaggtagtga aatgttagtt acgtatccag ctttatttta
    11101 ttatgacgac acagacggaa cagaagcgac ttattttgtc catttcccag attttgaata
    11161 ctcagctaca caaggagagg ggatttctga ggctttggct atggggtcgg agtggctagg
    11221 gataactgtt gcagatttga tagagagtga tggagagtta cctcagccat cagatatcaa
    11281 tagcttgtct ttaattgata atgatccttt taaagatgat gaagatttcg tgtcaaccta
    11341 tgaccttgat aaatctttta tttctatggt atctgttgat gtatcagaat acctaggaag
    11401 tcaggaaccc ataaaaaaga ctttgaccat accaaaatgg gcagataagt tgggacgaga
    11461 aatgggactt aacttttctc agactttgac agacgctatt gcagataaga aagttcaagc
    11521 ctaagattaa gcgtggtatc gtggtaatag tcaataaaaa agcacgtcca cttgtgctag
    11581 ttactcgcct attgatttta tagatttatt agccctttcg cgggcttttt atattgattt
    11641 ttatgagaaa tgaagaaatc agacttttaa gaaaatcact gataccaagg gtttaaatga
    11701 ggtaatatgg tataattagg acataaaata attttgtggt aagatggtag tatctatttt
    11761 agcatatttc cgagcaatcg gggcgattaa agagtcgcat agaaagagga caaaatggca
    11821 acatacggat ttttagatgt tttagaggaa gagttggaca agaactttcc ctttgacttt
    11881 gagattagct gggacaaacg caaccatgcg gtagaagtga gttttctatt ggaagcgcaa
    11941 aatgctgcag gtgtggagat ggtggatgaa gacggagagg ttttatcaga tgacattctt
    12001 tttgaggaag cggtcctttt ctacaatcct gccaagtcaa cagtcaatga ggaagactat
    12061 ttgactgtta ttccttacct gcctaaaaaa ggattttccc gtgaattttt agcttatttt
    12121 gcgcttttcc ttaaagatac tgctgaggtt gggctagatg tcctcatgga ctttttggaa
    12181 gacccagaag cagaggaatt tgtcatggaa tggaaccaag aagtctttga agaaggaaaa
    12241 gttggcttgg aagagggaga attttatcct tatccaagat actaggagtt ggttggaggt
    12301 gttatgaaga aaattgggac ttatttggtt tatgtgctag cttttgtctt tattatgcta
    12361 gcttttgctt gtggaacaat cgcatttgca gagttggggt attccgcagt tttagccttt
    12421 acttttggtt atgccttcgc tcttctaagc atgtatttaa tctttattct tcatgagctg
    12481 ggtcatgctt tttgtggcta cttgacaggc tatcggctgg tggcttttgg attaggacat
    12541 tttattttga ccaaaaagtc aggcaggttt catcttagta gaacagccat tctgaaaaat
    12601 gttggtgctc aatacattgg tttaaaagag gatgaaagcg atcaaagaat catcctgatg
    12661 ctttcaggag gcttgatggt tcatctcggc ttgatattat tggcgatagt gtttggattt
    12721 ttgacaagaa gctggtattt tgcagggact tggatttttc ttaatttatc tttcttccta
    12781 aataacattt tgccagtcga catcaccgat ggagcaaaaa tttgggaatt actacaacac
    12841 cctgaaaata ccaaatacgc ctacctggta ttgagacatt ctgctcaaac tttgctggct
    12901 cctcaagaat atgatttgaa agactttgtt caagcagttc ctgaggatgc acgagggagt
    12961 tttgcggatg gtgtattagg tatgcaaggt gaagtttcca ttttggaagg gaaggaagaa
    13021 gtagctaaac agcagtttca agcattactt gagaggacaa ctactccgat gatgcagaca
    13081 gttgcccaac tatccctgct tcatatcgcc ttgttggaag gagattttga gcaagcggag
    13141 caatatgcta gtattcgaca ggtcaaatcg tttctgtctc tcaaactgtc caatcttcaa
    13201 gtgatacaag cttggtatca atttaaggta aagaaagatg tagatcgaac tcgtaaggct
    13261 ctcaagattg ctagacagaa aatggatagt agtcgaatgt tgagggacga gaaacgctat
    13321 tatgaaaact tgttagcaga gctggaaaag gaaatgttag aaggaactga agatggaact
    13381 agaaatttct gatttcccag gctgtaagat tgctttgttt tgtggggata agctcttgac
    13441 tatcttacgt gatgataagg caagtattcc ctgggccaat atgtgggaac tgccaggtgg
    13501 tggaaagagt gtcagggaaa atctgatata gttttgtatt aaaagaatat cctttcaagg
    13561 ttattctttt ttaattttgt cccaaaataa ttgactttgt cccgaaaata tagtattatt
    13621 tgtgtaagga ggaatgaatc atgaaaaaat cagatgttct tgatttaata aagtatcatt
    13681 atgagggcag agagacagaa tttagaaatc aatccatagc aattgctcgg aacttcaata
    13741 aacatggtga tacacaaata gcacaatata ttatggggtt aatgtctcaa agtgatagat
    13801 tcatgccaca aatagaaaat cctagtgagt atttgactcc tgctaaacta gatattggtc
    13861 ctctcccatt gcctctttca attatgaatg atttaaaagg aataataaac gctgtcaatc
    13921 atcatattgg aataaataaa tttttatttg tgggatctcc aggtactgga aaaactgaaa
    13981 gtgtaaagca ggtggctaga cttattggca aggaattact tgtagtagat ttcagtcact
    14041 tagtagatag taagctaggg caaacagtca aaaacttagc aactcttttt aatgaaatca
    14101 ataatcttcc tttcaagcaa aattatatca tcctatttga tgaaattgat tccatagtgt
    14161 tggatagggt taatcagaat gatttaagag aaatgggacg agtgacttct gcctttttaa
    14221 aggagttgga taggctgtca ccagaaattg tattaattgc aacaacaaat ctttttgaaa
    14281 atcttgataa agcagttacg agaagattcg atgccataat tgactttgac cgttatactg
    14341 atgaagataa agtagaagta gctactatta ttttaaatga gttattaaag caatttaaga
    14401 atgtagctag agatttaaaa ttatttaaaa aaattatcaa tagtgccaat gtaataccga
    14461 atcctggtga tttgagaaac tcaataagaa cgtcattggc atttagtgac ccatcagatc
    14521 cgcatgatta ccaaaaacgc ctgctaagaa gtttgcataa tggtagaaat ttatctattt
    14581 ctaaattatc gaagctaggt tttactgtta gagaaattga gattttgaca ggtatttcta
    14641 agagtagtgt atcacgagag ttaagcgagg attaaaatga atacattatt gacattacga
    14701 ggtaaaagtt ttacacaaaa atcaagaaat aatggtatgg gaccaataac tatacccaaa
    14761 aaaacaataa ttacattgga acatttgaag tatcttcatt tttcattaga agagacgaaa
    14821 acatattggg agaaaaataa cattatcgat ggtattttga tcagtattta ctataatcga
    14881 attgttgcca aaagtaaccg tattaatggc tatttcaatg taggaggagg gaatccattc
    14941 cctaatgata ctattgtggg agcaaaattc aatgatgaga agacaaaaca tattgttact
    15001 cattatattt ctagagatgc tcttaataaa acaataaccg ttttaagtaa gataattgaa
    15061 gtttttgagg aacattttga tagagcgata acatgtgaga tgttttctga ttcatcaacg
    15121 tttgcttcta taaatttttc ggagtatggt atttcaaaaa gcaaatttca acagtatttg
    15181 agagattctt gctttattga gaattttgga gttgagcaca ctactgtttc tgatattcaa
    15241 aattctatcg tgacatttta tgatgtccat acagacattt ttagattact gaataaatta
    15301 aatattgata tttcagaagc taatatcatg aatcagacca cggtcttgct tgatgaaaaa
    15361 aatattgaat tgctactatc aaaagcgccc tatcttgtct ccatgattgt ggaagatttt
    15421 tctaagttat ctgtagatga tttttcttta gataataatg atttaaaaat aaatcttcca
    15481 tctccaatga acgaaccagt tgttggcgtt attgatacct tatttgacaa aagagtttac
    15541 tttaatgagt gggtagaata tcatgatttt gtctcacctg atattagcaa agatagccaa
    15601 gattacaagc atgggacagc tgtgacttct ttaatcgttg atggagccaa tttgaaccct
    15661 aatctggatg atggttgtgg aaattttaga gtgaggcatt ttggtgtgtc actacaaagt
    15721 ggatttaatt catttacaat cataaaacag ataaaagaga ttgtttctca gaatgctgat
    15781 attaaggttt ggaatctttc tttagggtcc aatgatgaaa taagagaaaa ttttatctct
    15841 gcagagggag cattattaga cgaaattcaa tttgagaatg atgtaatttt tatcatagct
    15901 gggacaaatg cttcagtaat aaatgggaaa cgcaaaagaa taggtgctcc tgctgattca
    15961 cttaattcta taatagtaaa ttctgttgat tttaataatc aatcagtgtc atattcacga
    16021 gagggtattg ttttatcatt ctttgttaaa ccagatgtgt catattatgg aggaggaaat
    16081 ggtgacttta taaatgtttg tgaacctcta ggattaggtc gtgtagctgg aacaagtttt
    16141 gcggcacctt ttattgcaag gaaaatggcc tatctcattc atattatggg acttagccga
    16201 gaagaagcga aggctttgct aattgatgca gcaataccct ggaatgataa gaaaacattc
    16261 actgatttat ctttgatagg aaatggtatt gttccaataa aaatggatga tattttatct
    16321 acacctgatg atgaaattaa atttattgtg tcagatatct ctagggctta tgatacctat
    16381 aattatgatt ttccagtacc tatttctagt gagagttatc catatgtagc taaagcaaca
    16441 atgtgttatt tccctaactg ctcaagaaaa caaggggttg attatacaaa tactgagatg
    16501 cagcttactt ttggtaggtt aaagtcagac ggtatcaaat cgataaataa agacaaccag
    16561 catgccgaag ataccccagg atatgtgaga gaaaacgctg ctagaaatat atttaggaaa
    16621 tgggataatg tcaagcatat tggagagtcg tttacttctc gaaagagagc taaagcaatt
    16681 ttaaatccat ctaatcctca gtgggggatg agtattaaaa caatcgaacg tttgaaaagc
    16741 ggtgatggac aaggagtacg ttttggtgta gtagttacat taaaagaatt aaatggggtt
    16801 aatcgtattg aagattttat tcagcaagct gaattacgcg gttggctagt taatagattg
    16861 caagtagaag ctcaagtaga cctctttaat agtttaaatg aagaaattga atttgaatag
    16921 tggatatttt atccttattt tagatcttag gaacacaaga gggcaaagat atgaatatta
    16981 cttatatagt tggaaatggt ttggatttgc aatatggttt gaaaacaaga tatagggatt
    17041 tctacgaatt ccaaaataag gtttatataa gtaggacaga gaacgaggaa aaatattcaa
    17101 atttcattta tgagtctcta ttttctgata aagtaaatga ttatgaaaat tggtcagatt
    17161 ttgaattgtc aattggtaaa ttgacaaaag ataacgacct catctcctca agtatagaaa
    17221 taaaggagaa atttatagat gattttagtg aagtagttga tgatttaaga gaatatctac
    17281 gcatacaaca agaaaaaaat cttgagaaag gtaatgcaat tgactttata agcactctag
    17341 atgatatgag gacctctctc cctgtaatta atcaacctgc tatcgataaa aaatacaatg
    17401 agaatccaca tcaggacgac attgtaaata tagtaacact taattataca catgtaatcg
    17461 ataaattata taatgggtca gcaaaatcat tcaggaatca attacgagct aatctttata
    17521 atttttatat tgagccacca attcatgcac atgggacggt ggatgtctgc acggttttag
    17581 gggttagtga tgaaatacaa atttcaaata gtttagaaga acaaaaggaa tccctaataa
    17641 aaagtttagt tttaaaaaac tatagagaaa atatggatgt aaagaatagt gatattataa
    17701 aaaattctga catcattatt ctctatgggg tttctttggg agaaactgat aggtatatct
    17761 ggagtcagat tgctgaacga tcaatcagcg gttctgttcc tgtcataatt taccactatg
    17821 tacctcattt tgatccgggg aatcccatta gagcaaaacg cctatataga aatgtagaag
    17881 ataaattcat acagaatagt ggtatagact tagagttaga gaaaaaatta agagacaacc
    17941 ttattgtcgt tattggtaag actatttttg atctaataga gaggtaattt taaaaaacaa
    18001 aatggaaaca tggcaagagt taaaagttac agtgaagcgt gagggagagg agttggtttc
    18061 caatctcttg attgagctgg gagcgcaagg tgttgcgatt gaagacagta tggactatgt
    18121 gggaaatgtt gaccgctttg gcgagatttt cccagaggtg gagcagcaag aagaaatcgt
    18181 agtgacagcc tactaccctg acacggtgga tgtgacagtg gttgaggcgg acttgcaggc
    18241 tcgcttagca gaattgacgg attttatgga tttgggagag ttaaaaatag gtacgactgt
    18301 cttggctgag gaagactggg cagataactg gaagaaatac tatgagccag ctcgtatcac
    18361 tcatgatttg accatcgttc cctcttggac agactatgag gcgactgcgg gggaaatgat
    18421 tatcaagctg gatcctggca tggcttttgg tactggaacc cacccaacta ccaagatgag
    18481 cctttttgcc ttggaacagg ttcttcgtgg tggcgaaacg gtgctagatg tggggactgg
    18541 ttcaggggtt ctctctattg ccagctcgct tctgggggct aaagaaattt tcgcctatga
    18601 cctagatgat gtagcagttc gtgttgctca ggaaaatatt gaacttaacc ctggaatgga
    18661 aaatatccac gttgctgctg gagacttgct taagggtgtt gagattgagg cagatgtgat
    18721 tgtagctaat atcttggcgg atattctcat tcatctgaca gacgatgcct atcgtttggt
    18781 caaggatgaa ggctacctga tcatgagtgg cattatcaag gacaagtggg acatggtgcg
    18841 cgagtcggct gagtcagctg gatttttcct tgaaacccac atggttcaag gggaatggaa
    18901 tgcctgtgtc tttaagaaaa ccaaggatat ttctggtgtg attggaggct agcatgcagc
    18961 agtattttgt aaaaggcagt gctatctctc ctgtcactat cgaggacaag gaaaccagta
    19021 agcatatgtt tcaggttatg cgcttgaaag aagaggatga ggttacctta gtctttgatg
    19081 atggcatcaa gcgcttggcg cgcgtgctgg atatggaaaa tcgtcagttt gagttggtcc
    19141 aagaattaaa tgacaatgtg gaactaccag ttcaagtgac catcgcatct ggatttccca
    19201 agggagacaa gctggagttc atcgctcaaa aagtaaccga actgggtgct agccaaatct
    19261 gggcctttcc tgcagactgg tcagttgcca agtgggatgg caagaaattg ggtaaaaaag
    19321 ttgaaaaact agaaaaaatt gcccttggag cagccgagca aagcaagcgt aatattgtac
    19381 caagtattaa acttttcgag aaaaaagcag attttctaac tcagctggac cagtttgact
    19441 ctatcatagt agcctatgaa gaatcagcta aagaaggaga agccgctgcg cttctgcaag
    19501 cagtcactgg tcttgaaaca ggagccaaac tgctctttat ctttggtcca gaaggcggtc
    19561 tctcacctgc agaaatcgaa agttttgaag ctaagggagc cgttttggta ggcctaggtc
    19621 ctcgtatttt gcgagcagaa acagcaccgc tttacgcctt atcagccctt agtgttttag
    19681 tagaattaga gaaataagag gaagaaaatg gaacaaaaac accgttcaga atttccagag
    19741 aaggaacttt gggatttaac agctctatac caagaccgtg aggatttctt gcgtgcaatc
    19801 gagaaagctc gcgaagacat caaccagttt agccgtgatt acaagggcaa tcttcacact
    19861 tttgaggatt tcgagaaggc ctttgcggaa ttggaacaga tctacattca gatgagccat
    19921 attggcaact atgcttttat gcctcagacg acggactata gcaatgacga atttgcaaat
    19981 attgctcaag ctgggatgga atttgaaaca gatgccagcg tagccttgac cttctttgac
    20041 gatgccttgg tggtagcaga cgaggaagtc ttggaccgtt tgggtgaatt gcctcacttg
    20101 acggcagcta ttcgtcaggc caaaatcaaa aaagcccact atctaggggc tgatgtggag
    20161 aaggccttga ccaatctcgg tgaagttttc tatagtccgc aggacattta tactaagatg
    20221 cgagctgggg attttgaaat ggctgacttt gaagcccatg gcaagaccta taaaaacagc
    20281 tttgtcacct atgagaattt ctaccaaaat cacgaggatg ctgaggttcg tgagaaatcc
    20341 ttccgttcct tctcagaggg acttcgtaag caccaaaata cggctgcagc agcctatctg
    20401 gctcaggtca agtctgaaaa actcttggct gatatgaagg gatacgactc tgtctttgat
    20461 tatcttctag ctgaacaaga agtggaccgt gtcatgtttg accgccagat tgacctcatc
    20521 atgaaggact ttgcaccagt cgctcagaga tacctcaagc atgttgccaa ggtaaatggt
    20581 cttgaaaaga tgacctttgc agactggaaa ttggacttgg acagcgccct caatcctgaa
    20641 gtgactattg acgatgccta tgatttggtc atgaagtcgg tagaaccttt ggggcaagaa
    20701 tattgtcagg aagttgctcg ttaccaagaa gagcgctggg tggactttgc tgctaacagt
    20761 ggcaaggatt ccggtggtta tgcggcggac ccatatcgcg tacaccctta tgtactcatg
    20821 agctggacag gccgtttgag cgatgtctat accttgattc atgaaatcgg gcattctggt
    20881 caattcatct tttcagacaa tcaccaaagc tatttcaacg cccacatgtc gacctattat
    20941 gttgaagcac cgtcgacctt caatgaattg ctactcagtg attacttaga gaaccagtct
    21001 aatgacccac gtcaaaaacg cttcgctctg gctcatcgct tgacagacac ctacttccat
    21061 aatttcatca cccacctctt ggaagccgcc ttccagcgta aggtgtatac attgattgaa
    21121 gaaggagaaa ccttcggagc aagcaaactc aacagcatta tgaaggaagt tttgacagat
    21181 ttttggggcg atgccattga aatcgacgac gatgcagctt tgacttggat gcgccaagct
    21241 cactactata tgggcttgta tagttacact tactctgctg gtcttgtcat ctcgacagct
    21301 ggttaccttc atcttaaaaa ctcagaaact ggagctgaag actggctcaa tctcctcaaa
    21361 tcaggtggta gcaagacacc acttgagtca gccatgatta tcggtgctga tatttcaaca
    21421 gacaaaccac tccgtgatac catccaattc ttgtctgaca cagttgacca gattatcgcc
    21481 tacagtgctg agttggggga gtaaggggaa tagaaaaatt ttctagaatg atgtttatgg
    21541 gcttgttaag cgtcattcta tttgtcttag ttgtagctat tttcaaaaat gtcgttgaag
    21601 gtcgtgatgt taaatttgaa cttactgtag aactggtatt gatagttatt tacgtcattt
    21661 tatatcagat tgtatttaat tgagaattca atattgagtc atttaagact gcctttttga
    21721 acagttgcta gaaatgtaga ggtattgacc atgtatcaag atttactaag aaaaatagca
    21781 gaagaaaaac caaattataa ccaggaagaa atccagtggt tgtttgatca tttgggaaat
    21841 ccttctccag aaattcgtga tgacctttca aatcaaggct tgcactatct ttcaaaagaa
    21901 aaggatacaa caggtttctc aagtcagtat ggttgggttc acgcttttgc acatggagcc
    21961 gatttactga cagaggtggt ttgtcatcca gactttccta aaaacagagt tcatgaagta
    22021 tttgatatac ttggccaact atttaaaaga atgtcgattc gctttacaga tgatgaggat
    22081 tggcgtttag caagagtgat ctatgaacct attttacaag ggaagttgga gcaagaacaa
    22141 gtagcttctt ggataaaaac tgttgatttt ccgatagaag aaagggagga tttttacaaa
    22201 ttttccaact ttagatcctg tctggtggaa gtctatgtcc aacttgacca gagaaatagt
    22261 ttacaagatg agttgaaaga agctatccag tcttttcaat actagagatt agctaatagt
    22321 tctatgattt tcaaaaaatt tccaatcaat tttcttgaaa ccctttccta cttttgatag
    22381 actagtatct agtttttgaa aaaaggagaa agaaaatgaa aaaatttgtt gctgagttaa
    22441 tcggtacgtt catgcttgtg ttcgttggga caggagctgt tgtttttgga aatggtcttg
    22501 atggccttgg tcaccttgga atcgcctttg cctttggttt ggcaatcgtg gtggcagcct
    22561 actcaatcgg aactgtttca ggtgctcact tgaacccggc tgtttcgatt gctatgtttg
    22621 taaacaaacg tttgtcatct tcagaacttg taaactacat ccttggtcag gttgttggag
    22681 ctttcatcgc ttctggcgct gtcttcttcc tcttggctaa ctcaggtatg tcaactgcta
    22741 gtcttggtga aaatgccttg gcaaacggtg tcactgtctt tggtggtttc ttgtttgaag
    22801 tcatcgcaac tttcttgttt gtattggtta tcatgactgt gacttcagaa agcaagggca
    22861 atggcgcgat tgctggtttg gtaatcggtt tgtcattgat ggcgatgatt cttgtcggat
    22921 tgaagattac tggactttca gtaaacccag ctcgtagctt ggcaccagct gtcttggtag
    22981 gcggcgcagc ccttcaacaa gtttggattt tcatccttgc accaatcgct ggtggagttc
    23041 ttgcagccct tgttgcaaaa aatttccttg gaacagaaga ataattgaaa ctcaaaaagc
    23101 cttgctcctc atcttgagga acagggcttt ttcgtatgat actcttcgaa aatctcttca
    23161 aaccacgtca gcttcacctt gccgtaggta tggttactga cttcgtcagt tctatcc
//
LOCUS       DAVKMU010000031        22815 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKMU010000031 DAVKMU010000000
VERSION     DAVKMU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.031"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            460..705
                     /locus_tag="VW343_001395"
     CDS             460..705
                     /locus_tag="VW343_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755936.1"
                     /translation="MGIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSASPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            862..1920
                     /locus_tag="VW343_001396"
     CDS             862..1920
                     /locus_tag="VW343_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755937.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEDGIQDAVASKQIVVINEFIACLNSRCVPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            2234..3211
                     /locus_tag="VW343_001397"
     CDS             2234..3211
                     /locus_tag="VW343_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755938.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKSLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            3588..3962
                     /locus_tag="VW343_001398"
     CDS             3588..3962
                     /locus_tag="VW343_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9755939.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            4170..5072
                     /locus_tag="VW343_001399"
     CDS             4170..5072
                     /locus_tag="VW343_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755940.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            5128..5481
                     /locus_tag="VW343_001400"
     CDS             5128..5481
                     /locus_tag="VW343_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9755941.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKSDCQGICAALAMSPGT
                     FIALAGSAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAGKIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            5478..6206
                     /locus_tag="VW343_001401"
     CDS             5478..6206
                     /locus_tag="VW343_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755942.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKAKEYELFKNSK"
     gene            7338..7841
                     /locus_tag="VW343_001402"
     CDS             7338..7841
                     /locus_tag="VW343_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755943.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQDGVFILEDFRNILLYNDVIERISLYEFIGDIFYSKITSCC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTD
                     VKEMKWL"
     gene            8128..10173
                     /gene="pspA"
                     /locus_tag="VW343_001403"
     CDS             8128..10173
                     /gene="pspA"
                     /locus_tag="VW343_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU9755944.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTLVRAEEAPVANQSKAEK
                     DYDAAVKKSEAAKKDYETAKKKAEDAQKKYDEDQKKTEEKAELVRKADEKRQKANLAV
                     QEAYVKFQEAQREFNESPSRKKSDAKKKLDDASAHIEEVKLKQKEADANFNKEQAKVI
                     PEASDLAVTKQKAEEAKKEAEVAKEKYDKAVQEVEVEKNKILEQDAENEKKIDVLQNK
                     VADLEKGIAPYQNKVAELNKEIARLQSDLKDAEENNVEDYIKEGLEQAIADKKAELAT
                     TQQNIDKTQKDLEDAELELEKVLATLDPEGKTQDELDKEAAEDANIEALQNKVADLEN
                     KVAELDKEVTRLQSDLKDAEENNVEDYVKEGLDKALTDKKVELNNTQKALDTAQKALD
                     TALNELGPDGDEEETPAPAPKPEQPAEQPKPAPAPQPAPAPKPEKTDDQQAEEDYARR
                     SEEEYNRLPQQQPPKAEKPAPAPKPEQPVPAPKTGWKQENGMWYFYNTDGSMATGWLQ
                     NNGSWYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGW
                     LQYNDSWYYLNANGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMAT
                     GWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV"
     gene            10573..11694
                     /gene="mnmA"
                     /locus_tag="VW343_001404"
     CDS             10573..11694
                     /gene="mnmA"
                     /locus_tag="VW343_001404"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9755945.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLATAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTNLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            11835..12290
                     /locus_tag="VW343_001405"
     CDS             11835..12290
                     /locus_tag="VW343_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9755946.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            12300..14213
                     /gene="mnmG"
                     /locus_tag="VW343_001406"
     CDS             12300..14213
                     /gene="mnmG"
                     /locus_tag="VW343_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9755947.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            14417..14494
                     /locus_tag="VW343_001407"
                     /pseudo
     CDS             14417..14494
                     /locus_tag="VW343_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(14592..16271)
                     /locus_tag="VW343_001408"
     CDS             complement(14592..16271)
                     /locus_tag="VW343_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9755948.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(16273..16506)
                     /locus_tag="VW343_001409"
     CDS             complement(16273..16506)
                     /locus_tag="VW343_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9755949.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            16786..17001
                     /locus_tag="VW343_001410"
     CDS             16786..17001
                     /locus_tag="VW343_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755950.1"
                     /translation="MYNLFAFAIGVRLLAFIVFVACVYGGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(17324..17476)
                     /gene="cibB"
                     /locus_tag="VW343_001411"
     CDS             complement(17324..17476)
                     /gene="cibB"
                     /locus_tag="VW343_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9755951.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(17478..17663)
                     /gene="cibA"
                     /locus_tag="VW343_001412"
     CDS             complement(17478..17663)
                     /gene="cibA"
                     /locus_tag="VW343_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9755952.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            17841..18524
                     /gene="tsaB"
                     /locus_tag="VW343_001413"
     CDS             17841..18524
                     /gene="tsaB"
                     /locus_tag="VW343_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9755953.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            18521..18958
                     /gene="rimI"
                     /locus_tag="VW343_001414"
     CDS             18521..18958
                     /gene="rimI"
                     /locus_tag="VW343_001414"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9755954.1"
                     /translation="MIEIKRIQQHPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            18948..19958
                     /gene="tsaD"
                     /locus_tag="VW343_001415"
     CDS             18948..19958
                     /gene="tsaD"
                     /locus_tag="VW343_001415"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9755955.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHELPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFANLDLNAKPSLAFDT
                     ME"
     gene            complement(19998..21304)
                     /locus_tag="VW343_001416"
                     /pseudo
     CDS             complement(19998..21304)
                     /locus_tag="VW343_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(21696..21776)
                     /locus_tag="VW343_001417"
     CDS             complement(21696..21776)
                     /locus_tag="VW343_001417"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9755956.1"
                     /translation="MLPLPTYSPEYNPIEKIWAYIKNISE"
     gene            complement(21977..22303)
                     /locus_tag="VW343_001418"
     CDS             complement(21977..22303)
                     /locus_tag="VW343_001418"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755957.1"
                     /translation="MEKVDRDKLKIYIETHPDAYLTEIAAEFNCPPTTIHYALKAMGY
                     SLKKSRTYCEQDPEKVNRFLKELNHLSYLTPIYIYETGVKTYFYLEYDRALSRQLVSL
                     EEDIII"
     gene            22540..>22815
                     /locus_tag="VW343_001419"
     CDS             22540..>22815
                     /locus_tag="VW343_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9755958.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFE"
BASE COUNT         7111 a         3887 c         4835 g         6982 t
ORIGIN      
        1 caactaggtc attagatttt attgaagata taaattaaaa acgaggttgg gaaaaaactc
       61 aatttgaaag tgagattgag taaatctttc tatctatata acgtatatca tcaatatttt
      121 tgaacacctg ataatatgca cttttcttat tttaaagatt ttttgccttt attcatttct
      181 tgaatgaatt ggagagatga ctttatttag gtgtttgaca ttactataga taggatttag
      241 cagtactaat atgaactcat attataggtg tagacttaaa ttttggaagc aagctaaagg
      301 atatcttatc taggtaacta agtgctataa caagatatat tggtggagtt agtatggcaa
      361 ctattactaa tgctcttaat attgtggcta ctgttgctga agtcttctca ttaggaggag
      421 ccattgcata tggtttagat atcgtagacg gtaaatttga tgggtattta tgggcgtaaa
      481 actatagcta tgaaacattt ttttgcagga attggtgaaa ttcatttttt gttattttta
      541 ttatatctaa gcatgtctgc tagtccaatt attggactag tgattggata tactaaaata
      601 aatctaataa attctattat tatagctctt ggtatgatat tggcattaat tttggtatta
      661 tcaagagtgt atagaaaata tttttataga gacgatgatg actaaagttt ttatcaataa
      721 tttgggctcc ttgtcaactg tagtgggttg aataaaagct aacgtctggg gaagggttta
      781 aagctttgct ttatcaaata ttgacttaca gagttgctct attgtattga tgcattctta
      841 gtaaataata tggtgtttta aatgaaagat gatcagaaat atttattagc gggactttat
      901 tcgcttctag ttgcaatatt ttattttcca ttgattgatt ccaaggggat ttttgtaagt
      961 atattgatgg cggttttact tttatattta atatacttca ttgcaacagt cattcatatt
     1021 gtgataatta agtttataag aaaaaaatct tttaagtatt tggttctata tccatttact
     1081 tatgatggga gctggagatt tcaacctata aacttactct actttccaga aatggtgaga
     1141 gatgtcattc cgattaattt agttcaagaa tattgccaag gacaacctta cggactgtta
     1201 aaaaagatgc tgaaaagaat tcgattgtca cgagagatat ctttactcct ggctaccatt
     1261 atcgtctatt tttttacgca tagaattcta cctctttcag tatttacgtt catattttct
     1321 tacatattgc tatttgcaca atcttatttg ggtggtaata ccgcttggat tggaaataga
     1381 agattaataa tagatgatga atttgaaaaa atcttgcttt caaaaagtta tataaaagaa
     1441 atatcatctg cacgatactc agaatacttg acatgtgaat acaaaaactt tacaccaatc
     1501 atattgttag ctatttttga aaatttgctt gactcctact tgcttcagaa tcagagtgag
     1561 gttgacttag atatttttta taaagtttta cctttgcttt ataaagaaaa atatactatg
     1621 ggattcaatt actttgtaag tttaaattat ttactatata aggttggatt tttgggaatc
     1681 atttacgata atgaagcatt aagagatttg tcaaaacaat atttaaacaa aaatatttcg
     1741 gaattacaag atggttcttt cgaggacggt atacaagatg ctgttgcctc taaacaaata
     1801 gtagttataa atgaatttat agcttgttta aattccagat gcgtgccatc ccaatatgat
     1861 agattttttt ataaagatag accttatatt ttttccagaa agagtcctat taaggggtga
     1921 aaatttgtta catttggatt aacaagtgtc ctagggatta gaaataactc tagtatagct
     1981 agatattttt gagtaactag aaatatcatt agtctaccca tatgattgcc agaaaagatg
     2041 gctgatgaat cttatttata ctgttgcagt aggcgttgtc taggataaat aagctatctt
     2101 gatgggggag attggaataa ttttagtaga aacattaaag gattatggac aagtagcgca
     2161 ggattaggtg atgcaatgtg ctaagttggc ttggtgttag cagtagtatt atttaacaat
     2221 ggaaagtaga ctaatgaaaa ataagcgtta tttttttgac acaattttaa taattttact
     2281 gcttataagc acgatatttt gtgtcagccc agtatttata aaattggaca tcttaggaac
     2341 tccctctcat gccatcttaa cctttgtatt ggcgataccg ttattctata ttttgtctca
     2401 atgcttacat actttgctat tactagtttc ctcaatcttt tgtaaattaa gaccaattta
     2461 tttttatttt atatttgtga tcattatagg agctaggaaa tactatcgaa ttctctttca
     2521 ccaactaatg ggatttagtc caggaatagc agtgttttat aaggagagtc aaacaactaa
     2581 gaatctgttt aaattttact acttcttgta ctttacaaca ctaatttcat actatttttt
     2641 ctttactttt gtgtatgaca aatcgttgct tttgcctctt attccatttt ctattatcat
     2701 agcattagta caaaaacttt atagaattga gaatcaacaa ttatttctgt taaaatcaaa
     2761 agtactaaca atactggaat ctaagaggga ttgtgagttt aatcttcagg attatcatga
     2821 aatttggaaa ttgcaatcaa aatctgagtt gccatgcgtg gctttatctt atatcagttt
     2881 gatcaagcca tatctttcag agagtgtgcg tgaacaaatt gatttattag aagttaagcg
     2941 atttaaaaaa ataaatcatc caatatcact ttatggtatg ttggatgtta taaaattaaa
     3001 tctatattta cggcattata atgaaaaaaa taaatatgaa tcaatgttaa agaaaatttt
     3061 agaggtaaga ccagactttg ttttaatcga gcagaatatt gatgatagtt taaatagctc
     3121 gcaaccctta tctttatctt tagccatttc agaaatccaa cttttgcttg aagtgtatat
     3181 ggggatcaaa catgtttcaa ttaggagata aaaccagaat aatatttgcc attatagggg
     3241 caagttgcat attagtcagt atgtatctta ttccacttat taaaactgag aataaatttt
     3301 gggaaatact tttatttata ttaggtgtat tgctaattgg aattgcgtta ctaagtcaaa
     3361 aaaaataaat tttgaaatag tattagtgag ttcgcaatta tttattgcta gtggtttaga
     3421 actgttaagt ttcaaacaat aattgatttt aaaccttagg taacctataa aaacaaataa
     3481 ggtagagaaa ggatatctta tataggtagc taagtgataa aaacacagtc gtctatcact
     3541 tgttaggaaa ctatcctaac tatgcttaat aaaaaaggag aaaataaatg gaactcgtat
     3601 taccaaataa ttatgttgct cttgagcaag aagagatgat gtatcttgat gggggatttt
     3661 ctattctgag atggcctgtt gcaacagcca ttaatatagc ttttaatggt gttttaggtg
     3721 gaggagcaat cagtctagtt agaaattata ttcgtaatta tggtttgcgg cgagttacaa
     3781 gcgcaattgc tggagcagct gcaagatatg ttggggtacg agttgcaaat agagtggcag
     3841 gatttgcact gtctgctatt aatggatttg cagcttggat gtcaattggc gatgctatta
     3901 caacaatctg ggccaacaat gatgtaaata ggagagaccc aaatttaaac gccttgtggt
     3961 aactagatat gggtattaaa atggaaaaaa tttttgttat tatttttttt gtatgtctat
     4021 ttatatcatc cataactttt ttagcctatg attttgttag cgaagaaata aaaaaattga
     4081 ttatttggat gaatgtcgtt tttttgattt taatcatagc aatgataatt tatccaaaat
     4141 taagaaaatg ataaggacac tgtaaaatta tgatacgtaa actaatcatt tttttattaa
     4201 tgctccctct ttttgtcttg tggctagagt tgcatgtcct agtgaataac ttactattga
     4261 atctagaatc tccccttgat tttgtgatta gtatgagtct tgctttctgt agtttgattt
     4321 tatcaaaaat agttttagac atactatacg cattgaaaga tttgtacaag aaagaagctc
     4381 ttattacgat ttttcctttt atttttatag gcaggaagaa agtaaatgtg aggttctcgc
     4441 cctatttttc attccatcgt aaaagtttgt cgcctgatga tttaagatct aggattatat
     4501 ggagttttat tcttgaaatt gctattatcc tagtatttat tttaaaaatt ccttttgcaa
     4561 tcatcatgct tactacaata tttttctgga ctataatgga tatcaatcat ctcgttttta
     4621 ataagacgga atttcttttc aatcaaaata aatgggaaaa agaagattcg tttgaaagtg
     4681 atttgacaaa tacgttaaag gataaaatac aaaaatcaga tctcagttat tctgacttaa
     4741 tgtctcttca gctgtatgat gcgatgaatc aatctacctt tttaacggat agtgaactct
     4801 ttgaggatat tctgagtaaa attgaagatt ctcataacac ccttctgtgt acaggtcttg
     4861 tcgagttact gctgtatgag atttctatta gtaacaataa caactggtcc aacaaggttg
     4921 ataaaattag gatacacctc atcagaatca atcaattaga ttttttctat tatacaagtt
     4981 ggttaaggca aaattttgat ttctgtatga atagagagta tcataaaatg aaatctcgga
     5041 aattattact gagtaataaa aagatagttt aagttcgtcc tatttgtaaa acaaatgaaa
     5101 caatttcaac taaggaggag aaaacaaatg gaacttgtat taccaaataa ttatgttgtg
     5161 attgatgaag aagaaatgat gtatcttgat gggggggctt atttaagcaa gagtgattgt
     5221 caaggaattt gtgcagcttt agctatgagc ccaggaactt ttatagcatt agctggatct
     5281 gcagttttaa ctaaaaaact aataaactat attaagtttg gaggtcttgg aggctggctt
     5341 attggtgcag cagcaggtgt attggctaca gcagcaggaa aaatagctta ctgtattgga
     5401 tatggtgctc ttaatagagg ttgtgatatt agcgggaacc cttatccttg ggatggattc
     5461 atatctgcga cagtaagatg aataatgtag ataagattcg aaaaatacat attatcgtat
     5521 gttggatgta tatattttta tcattcaggg cgattataaa tgacacggag tattttttgc
     5581 ttattttttt agcttttatc tactcaatag tatcactacc tctatattct gtgaaaaata
     5641 aaatagtatc tatctgtcta gccataaatt cgattctgtt aatgagtttc cccattttaa
     5701 tcaataagtt tttcccagaa agttttttga cttataccgt attaataagt gtttttatcc
     5761 tagagttagc aatctttcat ttaattgggg aagattttgc tactaaattg actaacgaat
     5821 ataagaaaat tagtcagttt agaagcaagg tgtctcaatc tccttggata aaatatttag
     5881 agatttctag ttttatatta actatatttc catctattct ttacggtaca gttgataatc
     5941 gtgtattggc tcttatcttt ttgataaaaa tttgtgtaga tactacgata aaatttttat
     6001 tcatcagatt atttgacaca agtactttaa tgaagaggag aatatttttt cttttcgcat
     6061 tggatgttat agcttactta tttttaggat atcttttagt gattcaaaaa gcaggctatt
     6121 tgttttcggt tttacttctt ttttctaatt tttcagttcc atttatcaaa gcaaaggaat
     6181 acgaattatt taaaaatagc aagtaataga aacataggtt aaatcttaga acggcttaaa
     6241 atcagatatg ataaagggaa atagtcttat ctaggtaact aagtgctaca aacacagtcg
     6301 aggatcactt gttaggatta cattttaatc atgcttaata agaatggaga attcctatgg
     6361 ggattgtatt actctgaagt ttcgaatacc ttagttctcg tgattttgag ctgttctagc
     6421 tttagaatcc ttcagaatgt aaaattttaa gcagtcaaag acttagaata gcctggaaac
     6481 atttatttta tagcaattct aggcttagaa ttgctcatat atatgtattt gagctgctcg
     6541 atagttagga cagctctatt ctgagaaatc tgagctatca atgcttcaga atagtatttc
     6601 gcaaatggtt tttattgaag aggtgattgt tttatgatag tctaaaataa aactgattta
     6661 atggagtaaa tggagaatcg tcacatgaaa catttttttg caggaattgg tgagataaaa
     6721 cttgttggct acttattgta tatatgtgta ggactagccc ctctttttca tatatatgtc
     6781 ataggttcgg aactgagttt tgtgaaaata gtgttatcta ttttgggagt tatatttgta
     6841 agtatgttaa ctattgctcg aatttatcgg acttttctat aaagaatagt ttggacttga
     6901 actgtatcta aaaacagagg ttggtcgatt atcagcctct ttcttgtcgt tgaaaagagt
     6961 gagatatatt gacttttgaa aaaaatgtca taattgagtt gggataaacc taaaagtaat
     7021 atagtagatt gaatctggaa tagtccgtta tgactgttaa aatatttcta gaaattaatt
     7081 tgactttcct aatcgatttg ttcacatctt gtttcaatct actatacttt ctcgttaaac
     7141 cgttcggcaa ttcatggtga catggacaca tagtagtaag cacattatgt ggcttggcag
     7201 agctaaaaac tgttcccttg cgataagcct aataagcaca acatagggag ttagagaagc
     7261 cgactctaat caaccacttt gggcagtagt gagaactgcc ccgtgctttt tattttgaga
     7321 aaatatggag tttgtcgttg aaattacttg attgtatttt ggactatcaa gaaagattca
     7381 atggaaaaac atgtcaagta tcaacgaatt ataagtattt agagactttc aaagtgaatt
     7441 tttgcttgac tgatttacat catttatttg gcttacacaa aatcacacga gattatgcta
     7501 gtcaaacaaa acctgctatt caagatggtg tttttatttt agaagatttt agaaatatcc
     7561 tattgtacaa tgatgtaatc gaaaggatat ctttatatga gtttataggt gatattttct
     7621 attctaaaat aacaagttgt tgcatcgtag ctaaggattt atctaaaaat actatgaaat
     7681 tggacgtcat attttttgag gataaaaata aaagatccgc agttttaggt ttacgaagag
     7741 acaaaagcgg agtatttaaa ccagttaccc tacattttac aagcgctaag aaatatgcta
     7801 aagttcgtaa aacagatgtg aaagaaatga aatggctata aagattgtcc gcaggcttaa
     7861 gcttgcgttt tttgttgttt aacgaaatta aaaaaacaag ctctctcatc ggaaatgttt
     7921 ttataggaat gaaggaagat gatgctatgt ttaaaaagag aatcgaaaag ggcaaaagta
     7981 gtgtctttat ctttttggag tagaagctta tgatatagaa atttgtaata aaaatgtaat
     8041 ataaaacatt tgacaaatat ttacgggggg agtatactta atataagtat agtctaaaaa
     8101 tgactatcag aaaagaggta aatttagatg aataagaaaa aaatgatttt aacaagtcta
     8161 gccagcgtcg ctatcttagg ggctggtttt gttacgtctc agcctacttt ggtgagagca
     8221 gaagaagctc ccgtagctaa ccagtctaaa gctgagaaag actatgatgc agcagtgaaa
     8281 aaatctgaag ctgctaagaa agattacgaa acggctaaaa agaaagcaga agacgctcag
     8341 aagaaatatg atgaggatca gaagaaaact gaggagaaag cagagcttgt tcgaaaagcg
     8401 gatgaaaaac gtcaaaaagc gaatttggca gttcaagaag catatgtaaa attccaagaa
     8461 gcacaaagag aatttaacga aagtccaagc agaaaaaaat ctgatgctaa aaagaaatta
     8521 gatgatgcat cagctcatat tgaagaggta aaattaaaac aaaaagaggc ggatgcgaat
     8581 tttaataagg aacaagcaaa agtaattccc gaagcaagtg acttagctgt tactaaacaa
     8641 aaagcggaag aagctaaaaa agaagcagaa gtagctaagg aaaaatatga taaggcagtt
     8701 caagaggtag aagtagagaa aaataaaata cttgaacaag atgctgaaaa cgaaaagaaa
     8761 attgacgtac ttcaaaacaa agtcgctgat ttagaaaaag gaattgctcc ttatcaaaac
     8821 aaagtcgctg aattaaataa agaaattgct agacttcaaa gcgatttaaa agatgctgaa
     8881 gaaaataatg tagaagacta tattaaagaa ggtttagagc aagctatcgc tgataaaaaa
     8941 gctgaattag ctacaactca acaaaacata gataaaactc aaaaagattt agaggatgct
     9001 gaattagaac ttgaaaaagt attagctaca ttagaccctg aaggtaaaac tcaagatgaa
     9061 ttagataaag aagctgcaga agatgctaat attgaagctc ttcaaaacaa agttgctgat
     9121 ctagaaaaca aggttgctga attagataaa gaagttacta gacttcaaag cgatttaaaa
     9181 gatgctgaag aaaacaatgt agaagactac gttaaagaag gcttagataa agctcttact
     9241 gataaaaaag ttgaattaaa taatactcaa aaagcattag atactgctca aaaagcatta
     9301 gatactgctc ttaatgaatt aggtcctgac ggtgatgaag aagaaactcc agctccagca
     9361 ccaaaaccag agcaaccagc tgaacaacca aaaccagcgc cggctcctca accagctcca
     9421 gcaccaaaac cagaaaaaac agatgatcaa caagctgaag aagactatgc tcgtagatca
     9481 gaagaagaat ataaccgctt gccccaacag caaccgccaa aagcagaaaa accagctcca
     9541 gcaccaaaac cagagcaacc agttcctgca ccaaaaacag gttggaaaca agaaaacggt
     9601 atgtggtact tctacaatac tgatggttca atggctacag gttggctcca aaacaacggt
     9661 tcatggtatt acctcaacag caatggcgct atggctacag gttggctcca atacaatggt
     9721 tcatggtatt acctaaacgc taacggcgct atggctacag gttgggctaa agtcaacggt
     9781 tcatggtact acctcaacgc taatggtgct atggctacag gttggctcca atacaacgat
     9841 tcatggtatt acctaaacgc taacggcgct atggctacag gatggctcca atacaacggt
     9901 tcatggtact acctcaacgc taacggtgct atggctacag gttgggctaa agtcaacggt
     9961 tcatggtact acctcaacgc taacggttca atggcaacag gttgggtgaa agatggagat
    10021 acctggtact atcttgaagc atcaggtgct atgaaagcaa gccaatggtt caaagtatca
    10081 gataaatggt actatgtcaa tggtttaggt gcccttgcag tcaacacaac tgtagatggc
    10141 tataaagtca atgccaatgg tgaatgggtt taagccgatt aaattaaatc atgttaagaa
    10201 catttgacat tttaattttg aaacaaagat aaggttcgat tgaatagatt tatgttcgta
    10261 ttctttaggt acctatctta tgatttcagg aagtgtcatt aaaaaaacga ctccttttct
    10321 ctaacttgaa aaatagatta gagaaaatgg gttgttttat ttattatagt tatttgaatg
    10381 aagataagaa gagggtatac tcacgtcatt cacatgatct gtatattgac tataagtttt
    10441 aaaaaacaat ttttaagctc ttccttgtct tctctaacca agcgtgttat aatgaatact
    10501 gctcaagcga ccttcaatcg tgaagcacac acgaccttca atcgtgaata aacgaataga
    10561 tggagactta ccatgagtga taactctaaa acacgtgttg tcgtggggat gagtggtggt
    10621 gttgattcgt cggtgacggc tcttttgctc aaggagcagg gctacgatgt gatcggtatc
    10681 ttcatgaaga actgggatga cacagatgaa aacggcgttt gtacggcgac cgaagattac
    10741 aaggatgtgg ttgcggtggc agaccagatt ggcattccct actactctgt caattttgaa
    10801 aaagagtact gggaccgcgt ttttgagtat ttcctagcgg aataccgtgc agggcgcacg
    10861 ccaaatccgg acgttatgtg caacaaggaa atcaagttca aggccttttt ggactatgcc
    10921 atgaccttgg gggcagacta tgtagcgact gggcattatg ctcgagtggc gcgtgatgag
    10981 gacggcactg ttcacatgct tcgtggcgtg gacaatggca aggaccagac ctatttcctc
    11041 agccaacttt cgcaagaaca actccaaaaa accatgttcc ctttgggaca tttggaaaag
    11101 cctgaagtac gcagactagc agaagaagca ggcctggcga ctgctaagaa gaaagattcg
    11161 acagggattt gttttatcgg agaaaagaac tttaaaaact tcctcagcaa ctacctgcca
    11221 gctcagcctg gtcgcatgat gactgtggat ggtcgcgata tgggcgagca tgcaggtctt
    11281 atgtactata caatcggtca gcgtggcgga ctcggtatcg gtgggcaaca cggcggtgac
    11341 aatgcccctt ggttcgttgt cggaaaagat ctaagcaaga atattctcta tgtaggacaa
    11401 ggattctacc atgattcgct catgtcaact aacctagaag ccagtcaagt ccactttact
    11461 cgtgaaatgc cagaagagtt tacgctagaa tgtacggcta aattccgtta ccgtcagcct
    11521 gactctaagg tgaccgtcca tgtcaaagga gacaaggcag aagttatctt tgcggaacca
    11581 caacgcgcga ttacaccagg acaggcagtt gtcttttacg atggcgaaga gtgtctaggt
    11641 ggcggtttga ttgacaatgc ttaccgcgat ggacaagttt gtcagtacat ttagattgac
    11701 aaattttctc aatttgctac aataataaca gcaatagaaa tgatggtcaa agctcatgga
    11761 tgttgcaggc ttttttgtcc tgcacttctt tgtagttttg actgtttttg tgtcgtttaa
    11821 gggaaaggac aagaatgact caacaagact ttcggacaaa agtagacaat acggtttttg
    11881 gtgttcgggc gacagccttg attgtccaaa atcacaagct tctagtcacc aaagacaagg
    11941 gcaagtatta cactatcggc ggtgcgattc aagttaacga aaaaacggaa gacgcggtag
    12001 tccgtgaagt gaaggaagaa ctaggtgtta aagctcaagc tgggcagcta gcttttgtgg
    12061 ttgaaaatcg ttttgaagtg gacggcgttt cctatcacaa tatcgagttt cattatctgg
    12121 tggatttgct tgaagatgcc ccgttgacca tgcaggaaga tgagaaaagg cagccctgtg
    12181 agtggattga cttggataag cttgaggata tccagctagt tccagccttt ttaaaaacag
    12241 ccctaccaga ttgggaaggc caactaagac acattcatct tgaggaatag gagagaaaca
    12301 tgacttatca ttttactgaa gaatacgata ttattgtaat tggtgcggga cacgctgggg
    12361 ttgaggcttc cttggccgct agccgtatgg gctgtaaggt cctgcttgcg accatcaata
    12421 ttgaaatgct ggctttcatg ccttgtaatc cctctatcgg tggttctgcc aaggggattg
    12481 tcgtgcgtga agtcgatgcc ctcggtggcg agatggccaa aaccattgac aagacttaca
    12541 tccagatgaa gatgctcaac acagggaagg ggccagctgt ccgtgccctt cgtgcgcagg
    12601 ctgacaagga actttactct aaggagatgc gcaagacggt tgaaaaccaa gaaaatctga
    12661 cccttcgtca aaccatgatt gatgagattt tggtggaaga tggcaaggtt gtcggtgtgc
    12721 gtacagccac ccatcaagaa tatgctgcta aggctgttat tgtgacgaca gggactgctc
    12781 tccgtgggga aattatcatc ggagacctca agtactcatc aggtcctaac cacagcttgg
    12841 cttctattaa cctagctgac aatctcaagg aactgggcct cgaaatcggt cgtttcaaga
    12901 caggaacccc tccacgtgtc aaggcttctt ctatcaatta cgatgtgaca gaaatccaac
    12961 caggagatga agcacctaat cacttctcat acacttcacg tgatgaggat tatgtcaagg
    13021 accaagtgcc atgctggttg acctacacca atggtaccag tcatgagatt atccaaaaca
    13081 acctccaccg tgcgcctatg tttacaggtg tggtcaaggg agtggggcct cgttactgtc
    13141 catcgattga ggacaagatt gtgcgctttg cggacaagga acgtcaccaa ctcttccttg
    13201 agccagaagg gcgcaatact gaggaagtct atgttcaagg actttcaacc agtctgcctg
    13261 aggatgtcca gcgtgacttg gttcattcca tcaaaggttt ggaaaatgca gagatgatgc
    13321 ggacaggtta tgctattgag tatgatatgg tcttgcctca tcagttgcgt gcgactctgg
    13381 aaaccaagaa aatctcaggt ctcttcactg ctggtcagac aaatggaaca tcaggttatg
    13441 aagaagctgc tggccaaggg attatcgcgg gtatcaatgc ggctctgaaa atccaaggca
    13501 agcctgagtt gattttgaag cgcagtgacg gttatatcgg ggtgatgatc gacgacttgg
    13561 tgaccaaggg aaccattgaa ccctaccgtc tcttgacaag tcgtgctgaa taccgtctca
    13621 ttcttcgtca tgacaatgct gatatgcgct tgactgagat gggacgcgag attggccttg
    13681 tggatgatga acgctgggct cgttttgaaa tcaagaaaaa tcaatttgat aatgagatga
    13741 aacgcctaga cagtatcaaa ctcaagccag tcaaggaaac caatgctaag gttgaggaaa
    13801 tgggcttcaa gccgttgaca gatgcggtga cagccaaaga attccttcgc cgtccagaag
    13861 tttcttacca agatgtggtg gccttcatcg gaccagctgc agaagacttg gatgacaaga
    13921 ttatcgaatt gattgaaaca gaaatcaagt acgaaggcta tatttccaaa gccatggatc
    13981 aggttgccaa gatgaaacgt atggaagaaa aacgcattcc agccaatatc gactgggatg
    14041 acatcgattc tattgcgacg gaagctcgtc agaagttcaa actcatcaat ccagaaacca
    14101 tcggccaagc cagccgtatt tcgggagtaa acccagcaga tatttctatt ttgatggtgt
    14161 atctggaagg taaaaatcgt agtatttcta aaactcttca aaaatcaaaa tgatacgtcg
    14221 tcggcttctt acgaatgagt tcaaagcttg gctttgattc atctccaacc tcccatagtt
    14281 ccccgaacta tgggagctaa ctcaagttat gctttcgctt agccttctag tctgattttc
    14341 attgagtttt aatctcaaaa gaaagcatag agaaaaacag ctccgaagac ggggctgttt
    14401 tgttgatttg tactcaatga aaatcaaaga gcaaactagg aagctagccg taggttgctc
    14461 aaagcactgc tttgaggttg tggatagaac tgacgaagtc agctcaaaac acagttttga
    14521 ggttgtggat agaactgacg aagtcagtaa catatatacg gcaaggcgaa gctgacgtgg
    14581 tttgaagagt attattcttc atctggtgtg aggatcatcg ggatgatgat cggttcacgt
    14641 tcggtatttt catagaggaa ggggcgaata gcgttgacaa tggcaccatt gacagattgc
    14701 acgctagcat ccttattttt cagtgcgata cgaatggcat tgaagaggat acgctggctt
    14761 tggcgaatca agtcgccaga ctctctcatg tagacaaagc ctcggctgag gatgtctgga
    14821 ccagatagaa tcatctgcga tttgaagtca acagttgcga ctgccagaac gacaccgtct
    14881 tcagatagat cgcgacgatc tttgaggaca gctgcgccaa tttcaccgat acgatttcca
    14941 tcgacataga tatcttgggc gttgaaatga cctgcgatac gagctgagtc agcagtaagg
    15001 gcaagcacat cgccattgct catgataaag atattgtcct tctcaacacc agtatccact
    15061 gctagtccag cgtggacttt ttgcatgcgg tattcaccgt ggacaggcat gaagtatttt
    15121 ggcttaatca agcagagcat gagtttttgc tcttgctgac caccgtgtcc agatgtatgg
    15181 atattgttta ctttaccgtg gataacttcg acaccagctt cagaaatgat gttaatcagc
    15241 ttgttgacgc tagtagtgtt tccagggatg ggactagaag agaagataac ggtatcacct
    15301 ggttgtaatt gtacttgacg gtgggttccg ttggcgatac gagagagggc tgccataggc
    15361 tcaccctgac tacctgtaca gaggataaga acttctcctg caggataatc tttgatttca
    15421 tttggctcga taaaggttcc cttaggagct ttgatgtagc caagatcgat tccgttgaca
    15481 atggcctttt ccatagaacg accaaagacc gcaatcttgc gtccagtctt aacagcagct
    15541 tctgttgcct gctggagacg gaagatattt gaggcaaagg atgcaaagat gatacgtcct
    15601 tcaatacctt ggataatctt cataatggac tgaccaacga ctttttcaga gttggtaaag
    15661 gttggtactt ccgcatttgt cgagtcagac aggagacaga gcacgccttc ttcaccaagc
    15721 gcagccatac gatgcaagtc cgcaggttct ccaactggag taaagtcgaa cttaaagtca
    15781 cccgtacaga cgattttccc ttgaggagta tgaatgacaa tccccaaagg ctctggaata
    15841 gagtgagtcg ttctaaagaa agttgccttg agatttttaa aggtcaactc ggtgttgtgg
    15901 ttgatttcgt aaagtttggc gttgcgcaag aggccgtgtt cttcgagttt cccacggatc
    15961 aaagccaagg caagcggtcc agcgtagata gggacatttg cttgcttgag taggaacgga
    16021 atcccaccaa tgtggtcctc gtgtccgtgt gtgattaaaa cagccttgac gcggtcgata
    16081 ttgtccacga tgtaagagta gtcaggaatg acatagtcga taccaagcaa gtcatcttct
    16141 gggaatttaa tcccagcatc gacgatgata atctcgtctt ggtattcaat tccgtaagtg
    16201 tttttcccga tttctcctag accaccgatg gcaaaaacgc cgacttcttc aggtttaaga
    16261 gtgtaggcca tattagaact ccgtaatttc gaaggcgcca gtttcttttt cgtaatcgag
    16321 caatttgtca gacaagagtt cgatatattc gatattgtac tctgggcgat tttcttcgac
    16381 aagttggcga gcagtgatac ggccctcaag ttctgagctg gcatcgatgt ctaggtaaag
    16441 cgcgcgtgtt gtttcacggc gtgggctacg ttcttttgtt tcttgataaa aaactttgta
    16501 aatcatatag ttcctttcta tttacaggtc agcttattcc aaacttttag cagagatttg
    16561 cttgatttca ttccattttg tgtctagatt gaccttgatt cgttacaatt atttcaatta
    16621 taccacaaaa tgaaaaatta gtaaaagacg gaaacgagaa agttagggga cgatttcttt
    16681 ggtaagcgtt tgcaaaaata aagaaaatgt attatgttga taacggaagt gaaatttatt
    16741 gcttgtcata attccatata gattttaagt caaggaggtt gccctatgta taacttattt
    16801 gcttttgcta tcggagttcg tttgctagct tttatcgttt ttgttgcttg cgtttatgga
    16861 ggaaatcttc tatttgccaa actggaacga cgacaaacca agttcttgtg gaaaatgacc
    16921 tttgtgactc tctactcgct ttttctcctc ctcgtgacct atgtcgtttg gtttgtattt
    16981 tactttgaat taaatgctta gatgccctgc ttatgctggg gttttttgtt tggggatgta
    17041 gagatagtag atttacgaca cgaaaattcc accccatcta tcaagatgag gtggtctttg
    17101 catttttcta acatgacatg acaatggaat tttcaaagct tattattttt atgggagtca
    17161 ttgttctgat caaaaataga gttgaagcat aaaaataaaa caatacaaac agataaatcg
    17221 aatggtaaag ggatggtagt aatgttcaaa tcattgagaa tgctgagcaa actgtaagag
    17281 atgaagacaa taaaatttat ccttctaaaa atgtgtttct tatctagcat gacttaccaa
    17341 actttttacg aaggtctttt ccaatggtat aacctgccat aaataaagcg ataccaacac
    17401 cgacaccaaa gccacccttg atttcttgca attccttatt agaaatttca cgataattgt
    17461 tcaaatcttt catcatatta atctcctcct aaaaaataac cagctgccaa accagaacct
    17521 ataagtgctg ttccacctgc aatagccttc caagatactg ctactccaaa gataaccaag
    17581 ggagcgagac cgccatcaat atctgataat tcattttcag ataaggataa aaattgattg
    17641 tcaagaatgt caaaatttgt cattgaactt tcctcctttt ttcttgttca cttttatatt
    17701 cggaaaagaa aaggaaaagt gagcattttt ttaaattatt tttagttaag tgagggcact
    17761 cttgtctggg ggcaaagaaa attcccatgt gccagctttt gtagtataat agtaagcaga
    17821 caaaaagata ggaatgtgtt atgaaagtat tagcttttga tacgtccagc aaggctcttt
    17881 ctctggctat tttagaggat aagcaggttc ttgccgagac gacgattaat attaagaaaa
    17941 atcacagtat tactcttatg cctgccatcg attttttgat ggcaagtttg gattggacac
    18001 ccaaggattt ggaccgaatc gtggtagctg aagggccggg tagctataca ggcttgcgaa
    18061 ttgcggtagc aactgctaag accttagctt acaccctgaa catcgagttg gttggtatgt
    18121 cgagtctctt ggctctggtg ccccaccaac aagaaggttt gtttgtcccc ttgatggatg
    18181 cgcgtcgcaa taatgtttat gcaggatttt atgaaaatgc caaacctgtc atggcagaag
    18241 cgcacctatc ttttgaagag gtgctagaaa aagtcaaggg tactagtcag gtaacctttg
    18301 tcggagaagt tggccccttt gttgagcaga ttcaaaaaca cttgccaagg actgattaca
    18361 aagaaacatt gcccaatgca gccaatcttg ctcttttagc ttgggacaag gaagcagact
    18421 ccttgcatga ttttgtgccg aactacctca agcgtgtcga agctgaggaa aactggctca
    18481 agaatcacac cgagtctggt gagtcctaca ttaaacgcct atgatagaaa tcaagcgaat
    18541 tcaacaacat cctgacctag ctcaagccat ctacgctgtt atggcagctg tttacctagt
    18601 cagtccttgg actctggagc aaatccaagc agatctgtcc caagaccaga cttggtatgc
    18661 attggcttat gatggggcag aagtgattgg atttctagct gtgcaggaga atctttttga
    18721 agcagaagtc ctgcaaatcg ctgtcaaagg agcttatcag ggtcagggaa ttgcgtcagc
    18781 cttgtttgct caattgccga cagacaagga aattttcctc gaagtcagac agtcaaatca
    18841 acgagcgcaa gcattttaca agaaagaaaa gatggcagtt atcgctgagc gaaaggccta
    18901 ctaccatgac ccagtcgagg acgccattat catgaagaga gaaatagatg aaggatagat
    18961 atattttagc atttgagaca tcctgtgatg agaccagtgt cgccgtcttg aaaaacgacg
    19021 atgagctctt gtccaatgtc attgctagtc aaattgagag tcacaaacgt tttggtggcg
    19081 tagtgcccga agtagccagt cgtcaccatg tcgaggtcat tacagcctgt atcgaggagg
    19141 cattggcaga agcagggatt accgaagagg acgtgacagc tgttgcggtt acctacggac
    19201 caggcttggt cggagccttg ctagttggtt tgtcagctgc caaggccttt gcttgggctc
    19261 acgaacttcc actgattcct gttaatcaca tggctgggca cctcatggca gctcagagtg
    19321 tggagccttt ggagtttccc ttgctagccc ttttagtcag tggtgggcac acagagttgg
    19381 tctatgtttc tgaggctggc gattacaaga ttgttgggga gacacgagac gatgcagttg
    19441 gggaggctta tgacaaggtc ggtcgtgtca tgggcttgac ctatcctgca ggtcgtgaga
    19501 ttgacgagct ggctcatcag gggcaggata tttatgattt cccccgtgcc atgattaagg
    19561 aagataatct ggagttctcc ttctcaggtt tgaaatctgc ctttatcaat cttcatcaca
    19621 atgccgagca aaagggagaa agcctgtcta cagaagattt gtgtgcttcc ttccaagcag
    19681 cagttatgga cattctcatg gcaaaaacca agaaggcttt ggagaaatat cctgttaaaa
    19741 ccctagttgt ggcaggtggt gtggcagcca ataaaggtct cagagaacgc ctagcagccg
    19801 aaatcacaga tgtcaaggtt atcatccccc ctctgcgact ctgcggagac aatgcaggta
    19861 tgattgccta tgccagcgtc agcgagtgga acaaagaaaa ctttgcaaac ttggacctca
    19921 atgccaaacc aagtcttgcc tttgatacca tggaataaag agtggctctt tgtcaagtgt
    19981 agtgggtagg cgaaaagcta caatctggag agtacgaaat tcgtactctc ttttttgatg
    20041 ttcaaagtca tcaaaatccg ttttttaaaa ttgttaaagt tccggaaacc aaaggctttg
    20101 cgcttgataa ccttaatcaa attattggtc gcttcaagtt tagcgttgga ataaggcagc
    20161 ttaagtgcgt tgataatctt atctctatcc cttaaaaaag tccaaaagac cgtttgaaaa
    20221 agcggatgaa ccgttggcag ttcttgctca atcaatccaa agaaatggtc ggcattcttc
    20281 tcttgaaaat gaaagagcag gagttgatag agttcgtagt aaacctgtaa tccctcagag
    20341 taagaaagca gcttatctcg tacttcccga tgggttaagt gcattcgaaa cgtgggctga
    20401 tagaatcgtt tctgacagag cttgaagcta tcctggtgta gtaaatcagt ccagtggact
    20461 gatttagccc gagcctagaa acgaaaaagc gagggttcca aaagcgcttg agcgcccgat
    20521 actccaagga ttttcggtca aattggttca tgatttggat tcgtactcgg ttcatagctc
    20581 ggctcagatg ttggacaatg tggaagcggt caagaacaat cttcgccttt ggaaagagtt
    20641 gcttagcgag ccgataataa gggctgtaca tgtccatggt gatgacctcg accagctccc
    20701 gaacctctct ctgatagcgt tggaaatggt ttcgaatcac cgcatgagtt cgcccgtcta
    20761 aaattgcgag gatggatttg gactcaaaat cttgggcaat gaagctcatt ttgctctttt
    20821 tgaagctata ctcatcccaa ctcataactt ttggcaactt ggtccaatcg gtttcaaact
    20881 taaattccct cagttttcgg atgacggtgg aagttgagac cgccaatctg tgggcaatct
    20941 cagtcataga ctgcttttca aggaggagct gagcgatttt ttgtctcacc atgttggaaa
    21001 tctggcaatt tttcttgaca atggatgttt gagaaacgac caccttaagg caattcttgc
    21061 actgaaagcg gcgctttttg agatgcagta ccgtgggtaa accctgacag tcgagaagcg
    21121 gaattttaga ggctttctgg aagtcgtatt tgatcatctt cccttgacaa tgaggacaag
    21181 gaggagcggg ggaatccaac tttgcctgaa cgactagatg ggtttggtat ttcagaacag
    21241 acaagatttt gatatttttg tcttccaatc cgagcaaatc tgtggtattc ttaagttgtt
    21301 ccatatgaac cctttctaat gagttggtta gacgcttttc attataggtc atatggggct
    21361 ttttttctac aagaaacgac cctataattc ctggggtggg attacccact acagaaatta
    21421 tagagccaaa gcattccaaa gtcttgtctg actctggaat gttttctgtt cacagttgtt
    21481 ttgtaactgc ctaataagta accgcgctat aacgaggtat ctaccgagat tgtcaattct
    21541 ccagtatact gttataaaaa acaaaactgg aggatttttt tatgtccccc catcgtccca
    21601 tcgactgttc cgtagctgcc caataacgga gtatagtcgg ctgaaacaag aataggacaa
    21661 aagtgcctca agaaaagcat cgtaatttga caatattatt ctgagatgtt tttgatgtaa
    21721 gcccatattt tctcaatggg attatactcg ggtgaatagg taggaagtgg taacagtcta
    21781 tgccctgctc cttacacatg ttcattctgt gaaaccttgc attgtccata atgataaggg
    21841 atggtgtatc taaagtaggt agtaagaatt tttggaagca agcttcgaag gagccatagt
    21901 atattgaaac aaaaaagggc attagtactt ctataacagt atcgcaatct ctggtgtgcg
    21961 ttgtctcgat cttaattcaa ataattatat cttcttccag agagactaac tgcctgctca
    22021 aggctcgatc atattcgaga taaaaatagg tcttaacccc tgtctcataa atataaatag
    22081 gagtcaggta gcttaagtga ttcaattctt taaggaaccg atttactttt tctgggtctt
    22141 gttcgcagta ggtacggctc ttttttagac tatatcccat agcctttaga gcgtaatgaa
    22201 tagttgttgg aggacagttg aattcagcag ctatttcagt caaataagca tcgggatgag
    22261 tttcaatata gatctttaat ttatctctat caactttttc aattaatagt ggaagttttc
    22321 ttgaagttag tgtattgaaa cgtttttata cagtgctaaa atttgaagtg tagtgcagat
    22381 gtatttgtaa tagtattata gcaattcttt ggagaaggtc gagacaagag aaaataggta
    22441 tctttgagat ataagcatga tttactataa aattattgag ttattcataa aaatttctct
    22501 atcaaatatc ataatgcgtc gtttaaggag atggttacaa tgcaatatag ttgtggaaaa
    22561 ataaatatta atatccctga tggatatgga gacattaagg atatagtgtt ttcggctcat
    22621 attatagtta ggtataataa tggacattgt ggaggtatag atccgcatat aattggactt
    22681 tgtaaaaagc agatcagaag gatgtctcta tatcctattt tgataatcgt atctagagat
    22741 tcaaaggtta ttgatgatta taaaaattta gatattgcct atgttgattg tactcaatgt
    22801 tcaaataatt ttgaa
//
LOCUS       DAVKMU010000032        22600 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKMU010000032 DAVKMU010000000
VERSION     DAVKMU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.032"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            162..815
                     /locus_tag="VW343_001420"
     CDS             162..815
                     /locus_tag="VW343_001420"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9755959.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            825..1289
                     /locus_tag="VW343_001421"
     CDS             825..1289
                     /locus_tag="VW343_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9755960.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1302..2570
                     /locus_tag="VW343_001422"
     CDS             1302..2570
                     /locus_tag="VW343_001422"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9755961.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2584..2844
                     /locus_tag="VW343_001423"
     CDS             2584..2844
                     /locus_tag="VW343_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9755962.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2855..4216
                     /gene="pdrM"
                     /locus_tag="VW343_001424"
     CDS             2855..4216
                     /gene="pdrM"
                     /locus_tag="VW343_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9755963.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4370..5338
                     /locus_tag="VW343_001425"
     CDS             4370..5338
                     /locus_tag="VW343_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9755964.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5354..6346
                     /locus_tag="VW343_001426"
     CDS             5354..6346
                     /locus_tag="VW343_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9755965.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6564..7607
                     /locus_tag="VW343_001427"
     CDS             6564..7607
                     /locus_tag="VW343_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9755966.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7671..9356
                     /gene="lpdA"
                     /locus_tag="VW343_001428"
     CDS             7671..9356
                     /gene="lpdA"
                     /locus_tag="VW343_001428"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9755967.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9420..10409
                     /locus_tag="VW343_001429"
     CDS             9420..10409
                     /locus_tag="VW343_001429"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9755968.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10482..11552)
                     /gene="xerS"
                     /locus_tag="VW343_001430"
     CDS             complement(10482..11552)
                     /gene="xerS"
                     /locus_tag="VW343_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9755969.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(12060..13610)
                     /locus_tag="VW343_001431"
     CDS             complement(12060..13610)
                     /locus_tag="VW343_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9755970.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(13626..14405)
                     /locus_tag="VW343_001432"
     CDS             complement(13626..14405)
                     /locus_tag="VW343_001432"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9755971.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKEN"
     gene            complement(14392..15243)
                     /gene="ylqF"
                     /locus_tag="VW343_001433"
     CDS             complement(14392..15243)
                     /gene="ylqF"
                     /locus_tag="VW343_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9755972.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWCQYFESQEIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(15535..20784)
                     /locus_tag="VW343_001434"
     CDS             complement(15535..20784)
                     /locus_tag="VW343_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9755973.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVIGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVQPALPEAVVTDKGEPEVQPTLPEAVVTDKG
                     EPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEP
                     ISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVKEVGEDGERQIVT
                     SYEELHGKKISEPVETVTILKEMKPKILVKGTKENPKEKTVPVLTLTKVTEDAMNRSA
                     NLNYELDNKDNAEISSIIAEIKDGDTVVKKVDLSKEKLTDAVQNLDLFKDYKIATTMI
                     YDRGQGSETSKLDEKTLRLELKKVEIKNISSTNLVKVNDDGTEIPSDFMSEKPSDEDV
                     KKMYLKITSRDNKVTRLAVDKIELVTEKEKELYKITASAQDLIQHVDPSKTRNEYIHY
                     IEKPVPKVNNVYYNFNELVRDMQEHPNDEFKLGADLNATNVSAFGKSYVTKDFKGKLL
                     SDGDNHYTIHNLSRPLFGNVIGGTIKNINLGNVDINMPWANQVAAVANIIKGGTTIEN
                     VKVKGNIVGKDWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVE
                     RAYVNANLIGKKAKAAGIAYWTQNEGNNNTVRQEGAIKKSIAKGTIQVTEAIESGGVV
                     GSMKHHGSVEDSVSMMKVPNGEIFYGSSDIDYDDGYWTGDNVRRNYVVIGVSDGHSSY
                     QRSKDKNRIRPISEEEAKSKIEATGITADKYEINEPVVNRLNRLTRREDEYKSTQDYK
                     VDRDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDI
                     DHVMIHYADKTKEIKAVHQKESKVAQVREYSIDGLDDIVYTPNMVDKNRDQLIKDIKD
                     RLATVELISPEVRALMDKRDTSRDPNANSDERKNGYIRDLYFEESFSETKANLDKLVK
                     SLIENADHQLNSDEAAMKALVKKVDENKAKIVMALTYLNRYYDIKYGDMTIKNLMMFK
                     PDFYGKSVDLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFT
                     NDTDLNDWFIHAAKNVYIVEPKTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAH
                     MFLISTYNTMAYSSFEKYGKYTEAEREAFKDKIKEVAHAQQTYLDFWSRLALPSVRDQ
                     LLKSQNRVPTPVWDNQNYHNVEGVNRMGYDKNNKPIAPIRELYGPTWRYHTTNWYMGA
                     MASIFQDPNNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAY
                     AQGMLQTPDKSGNGEYGALGLNMAYHRENDGDQWYNYDPDKLKTREDIDRYMRNYNDA
                     LMMLDHLEADAVIPKLHGNISRWFKKMDRQYRKNGELHQFDKVRELTEDEKKKIVINN
                     IDDLVNNNLMTKHGAPSDRTYNPEDFDSAYVNINIMTGIYGGNTSQGAPGAASFKHNT
                     FRMWGYFGYENGFISYASSKYQGEADKTNKKLLGDDFIIKKVSKDKFNNLEEWKKQYF
                     KDVKSKAEKGFTAIEIDGRQITNYAQLKTLFAEAVQKDIDGMSDPKIKDHFKNTVDLK
                     SKVFKALLKNTDGFFNKLFKEDI"
     gene            complement(20828..>22600)
                     /locus_tag="VW343_001435"
     CDS             complement(20828..>22600)
                     /locus_tag="VW343_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9755974.1"
                     /translation="ITYNVLLAKNYGTESLFKALEGYRKVFLPTISNNEWFKKQTKAY
                     IVEEKSTIEEGREKQGKEGTKYSIGVYDRLTNPSWKYQSMVLPLLTLPEEKTVFMIAN
                     ISTIGFGAYDRYRSSEYPKGEKLNKFVEDNAKEAAKRFRDHYDYWYKILDNDNKEKLY
                     RSILVYDAFKFGTDKDKDKVTHQATFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAF
                     YYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDP
                     TITINSVLKYEDSENSTRLQVADPTQRFNSAEDLHNYMHNMFDVIYMLEYLEGKAVAN
                     LETNQKYELLRKIENKFDLDQDGNNVYATNVVRRLTMDEVNKLNSFDSLIENDIITSR
                     GYKDQEYKRNGYYTIDLFSPIYSALSGEKGTPGDLMGRRIAFELLAAKGYKEGMVPYI
                     SNQYEKDAKAAGSKINSYGKEVGLVTDELVLEKVFNGQYKTWTQFKKDMYKEREKQFS
                     KLNRVNFINPNNPLSRQRNVSVTDIGVLERMIVEAVRDDAQDDVAKFYPETNSRVLKL
                     KKAIYKAYLDQTNDFRSSIFENKK"
BASE COUNT         6644 a         4396 c         4360 g         7200 t
ORIGIN      
        1 ttaaaaagta attataaaaa atagccagtc tttataaact gtattcacgt attcaaatcg
       61 tatatatagt cgatttatta aatctttaga taaactgtaa aaagtggttt ccatagccac
      121 tttttgctat aatagaggca gtaaaaatga aggagtcggc tatggaacac tcgtcttggc
      181 atgctttgat taaggcgcaa ttacctgagg gttatttcgg gaaaatcaat cagtttatgg
      241 agcaggtcta ttctcagggg attatttatc cacccaagga aaaggttttt caggctctct
      301 tgacaacact gcttgaagaa gttaaggtgg taattctagg gcaagacccc tatcacggac
      361 caggtcaagc gcagggcttg agtttttctg tacctgactc tatcccagct ccaccatcct
      421 tgcaaaatat cttgaaagaa ttgtcagatg atatcggagt taagaaatct catgatttga
      481 cagcttgggc tgagcaagga gtcttgcttc ttaatgcttg tttgactgtt cctgctggac
      541 aggccaatgg tcatgctggt caaatctggg agccttttac tgatgctgtg attcaggtgg
      601 tcaatcatct agatagacca gtcgtttttg tactctgggg agcttatgca cgtaagaaga
      661 aggcattagt taccaatcct catcacttga ttatcgaatc agcccatcca agtcctttgt
      721 cggtttatag aggattttgg ggttccaagc ccttttccaa ggccaatgca ttcttaaaag
      781 agacaggaca agagccaatc gattggctta gataaggaga aaatatgcct cagttagcga
      841 cgatttgcta cattgataat gggaaagaac tgctcatgct ccatcgtaat aagaaaccca
      901 atgatgtcca tgaagggaaa tggattggtg tgggtggtaa gctagagaga ggagagacgc
      961 cccaggaatg cgcggcgcgt gaaatccttg aagaaacagg gctcaaagcc aagccagttc
     1021 taaaaggtgt catcactttt cctgaattta caccagattt agactggtac acctatgttt
     1081 ttaaggtgac ggagtttgag ggcgacttga ttgactgcaa tgaggggacg ctagaatggg
     1141 ttccctatga tgaggttttg agcaagccga cttgggaagg tgaccacacc tttgttgagt
     1201 ggcttttaga ggataaaccc ttcttttcag ccaagtttgt ttatgatggg gataaattgt
     1261 tggataccca agttgatttc tatgaataaa ggagaaagca gatgctacta atcaaaaatg
     1321 gtcgtgtaat ggatcccaag tctggtttgg atcaagtttg tgatgtctta gttcaagatg
     1381 ggaaaattat caaaattgcg tctgagatca cggaagaagg agcagaaacg attgatgcta
     1441 ctggtcttgt ggttgctcct ggcttggtcg atattcatgt tcatttccgt gaacctggtc
     1501 aaacacataa agaagacatt catactggtg ccctagcagc cgctgcaggt ggttttacta
     1561 ctgtcgtcat gatggctaat accagtccaa ccatttcaga cgtggagact ttgcaagcag
     1621 ttctccagtc agctgccaaa gagaagatta atgtcaagac agttgcgacc attactaaaa
     1681 actttaatgg taaaaacttg actgacttta aggcactctt agaagctggt gcggttggtt
     1741 tctctgatga cggtattccg cttgagagca gtaagattgt caaggaagcc atggaggaag
     1801 ccaaaaaact caataccttt atcagccttc atgaggaaga tccaggtttg aacggtgttc
     1861 ttggctttaa tgaaaatatt gctagagaac atttccatat ctgcggtgct actggggtgg
     1921 ctgagtacgc tatgatggcg cgtgatgtca tgattgccta tgcaactaaa gcccatgttc
     1981 acatccagca tttgtctaag gaagaaagtg ttaaagtagt ggagtttgct caggggttag
     2041 gtgcagaagt cacagcagaa gtagcgccac agcatttctc taagacagaa gcacttcttt
     2101 taacacaagg tagcaatgct aagatgaatc caccgcttcg tttggaatca gaccgtcgtg
     2161 ccgttatcga aggtctcaaa tcaggtgtca tcacagttat tgcgactgac cacgcgcctc
     2221 atcatgtaga tgaaaaaaat gttgaggata ttaccaaagc gccatctggt atgactggct
     2281 tagaaacatc cctgtctctc agcttgacct atttagtaga agctggtgag ttgagcttga
     2341 tggaattact tgaaaaaatg acatacaacc cagccaagct ttacaacttt gaagcaggtt
     2401 acttggctga gaatggtcca gcagatatca ctatttttga tgccaaggct gaccgctttg
     2461 tggactccca ttttgcttcc aaagcagcta attcaccatt catcggtgaa accttaaaag
     2521 ggcaggttaa atataccatc tgtaagggac aaatcgtcta tcaagcttga taggagttag
     2581 aatatgaatt attttcgaaa acgtagggag agacaagcga aatctaatag tggaatttac
     2641 tgtcccgcag ctaatcgttc caaaattcaa tatgaaacta aagaaaaagc tgaccatgcg
     2701 gttcaatatg ataacggagg tttagttaga agctattatt gtcgtacatg tgcgtgttgg
     2761 catactactt caaaatctaa taaacctccg ctaagtttga aaaactatgg tctgaaactc
     2821 tttggtttag ataaatcaga atagaaagag aagtatgtat aagacaaagt gtttacgaga
     2881 gaagttagta ttatttttaa aaattttctt cccaatcctg atctaccaat ttgccaatta
     2941 ttctgcctct tttgttgata ctgcaatgac aggtcaatac aacactatgg acttggctgg
     3001 tgtatctatg gcaaccagta tctggaatcc tttctttaca tttctaacag ggattgtgtc
     3061 agccttggtg cctatcattg gtcaccatct tggtcgaggc aaaaaggaag aagttgcgtc
     3121 tgatttttac caatttattt atttggcctt gggcctatct gtggtcttgc tggggatggt
     3181 acttttcttg gcaccaataa tcttgaatca tattgggtta gaagcagcag tagcggcagt
     3241 agcggttcgc tatctttggt ttttatctat cgggattatc cccttgttgc tctttagcgt
     3301 cattcgttcc ttgctggatt cgctgggctt gaccaaactg tccatgtacc tcatgctttt
     3361 gttactccct ctcaatagcg gatttaacta tctcttgatt tacggtgcct ttggtgttcc
     3421 agaactggga ggggctggtg ctggtttagg aacatccttg gcctactggg tcttgcttgg
     3481 gatttctgtt ctggttttat ttaaacagga gaagctcaaa gccttacacc ttgagaaacg
     3541 aattccactt aatatggata acattaagga aggagttcgt ttaggtctgc ctattggggg
     3601 aactgtcttc gcggaagtgg ctatcttttc agtggttggc ttgattatgg ctaagttttc
     3661 gtccttgatt atagctagtc accagtcagc tatgaacttt tcaagtctta tgtacgcctt
     3721 tcctatgagt atctcatcgg ctatggctat tgtcgtttcc tatgaagtgg gagccaagcg
     3781 atttgatgat gcgaaaacct atattggtct aggaagatgg actgctctca tttttgcggc
     3841 cttcacccta accttccttt acatttttag gggaaatgtg gccagtcttt atggtaacga
     3901 cccaaaattt atcgatttga cagcgcgttt tttaacttat agtcttttct tccagttagc
     3961 agataccttt gcggcaccgc ttcagggaat tttgcggggg tataaggata cagttattcc
     4021 tttttacctt ggtttgcttg gttattgggg cgtagcaatc cctgtggcta cgctatttga
     4081 ttccctaaca gattttggag cttattctta ctggatcggc ttgattatta gtttgattgt
     4141 gagtggggcg ctctaccgtt ggcgtttaac tgtgattatg aagagatttg aatctttagc
     4201 aaaatccaaa cgctaaaaag tttgtgaaaa aatattcttg acaagaaaat cccttgtttt
     4261 cacggggttt tcttttttat tttgtgaaat ttcttttaga aaaactttga cagtatttgc
     4321 caaaagcggt aaaatagtaa ggtaagaaga aagttagaaa ggcaagaaga tgtcaacttt
     4381 agataaaaat cttttgctag aaatgttccg taagatggaa gaaatccgtc gcatggactt
     4441 aaaaattgca caattagtaa agaaagggaa agtgccagga atgacgcact tttctgttgg
     4501 tgaagaggca gctaacgtgg gggctatgtt agctctcaat ccagatgatc tgattacctc
     4561 aaaccaccgt ggacacgggc aagctattgc taaagggatt gacctcaacg gaatgatggc
     4621 tgaaatcctt ggtaaataca ctggaacctg taaagggaaa ggtggatcta tgcatatcgc
     4681 tgaccttgat gctgggaacc ttggtgccaa tggtatcgta ggtggtggta tggggatcgc
     4741 tgtcggtgca gccctcagtc agcaaatgca aaataccggt aaaatcgttg tctgcttctt
     4801 tggagatggt gcgaccaatg aaggtgtttt ccacgaagca gtgaacatgg cttctatctg
     4861 gaacctgcca gtcattttct attgcattaa caacggttac ggtatctctg cggatatcaa
     4921 gaaaatgacc aatatagaac atatccatca acgtagcgcc gcttatggaa ttcctggaat
     4981 gttcatcgaa gatggtaaca atgtcatcga tgtctatgaa ggatttcaga aagctgtgga
     5041 ccatgttcgc agtggcaatg gttcagtctt gattgaaagt gtaacttatc gctggctcgg
     5101 tcactcatca tctgaccctg gtaaatatcg tacgcgtgaa gaagtggaat tgtggaaaca
     5161 aaaagatcca atcgaaaacc tccgcaatta ccttattgaa aataacattg caagtgccga
     5221 agaattggaa gaaatccaag cgcaagtaaa ggaagcagta gaagcttctg ttaaatttgc
     5281 agaggaaagc ccattcccac cgcttgaatc agcctttgaa gatatttacg cagactaagg
     5341 agaaagagat aaaatggaaa caaaaacaat gtccttccgt gacaccatta tccttgctat
     5401 gtctgaggaa atgcgtcgcg atgaaaatgt gttcttgatg ggagaagatg tcggtgtctt
     5461 cggaggagac ttcggtactt ctgttggaat gcttgaagaa tttggtccag aacgtgtccg
     5521 tgactgtccg atttctgaag ctgccatctc tggagcagca gcaggagcag ccatgacagg
     5581 acttcgtcca atcgtcgata tgaccttcat ggacttctct gttattgcca tggacaatat
     5641 cgtcaaccaa gctgctaaaa cacgttacat gtttggtggt aaaggtcagg ttccaatgac
     5701 tgttcgttgt gcagctggta acggagttgg ctctgccgcc cagcactcac aatctctaga
     5761 gtcttggttt actcacattc caggacttaa ggttgtggca cctggaacac ctgcggacat
     5821 gaaaggcctg cttaagtctt ctatccgtga taacaaccca gttattatac ttgagtacaa
     5881 gtcagaattt aaccaaaaag gggaagtgcc agttgatcca gactacacaa ttccacttgg
     5941 ggttggtgaa atcaaacgcc aaggaacgga tgtaacagtt gttacttatg gaaaaatgct
     6001 tcgccgtgtg gttcaagctg ctgaagaatt agcagaagaa gaaatttcag ttgaaattgt
     6061 tgacccacgt acccttgttc cgcttgataa ggatatcatc ataaactcag tgaagaagac
     6121 tggtaaggtt attctggtca acgatgccca caaaacaagt ggctatatcg gtgaaatttc
     6181 agctattatt tcagaatcag aagcatttga ctatctagat gcaccaatac gccgttgttc
     6241 aggagaagat gtgccgatgc cttatgcaca aaacctagaa aatgcaatga ttccaacagt
     6301 tgaaagtatc aaagatgcaa tccgaaaaac ttataacaaa gactagagtt acataatata
     6361 agttatgtaa ctaaaataaa gctgacgaga aactttgtat cttttaggtg cagagctctt
     6421 cgtcattaat atcttatatt cgagcacggg ctaaaagctc ggaaaaaaga taaatctcct
     6481 tggcttcatc gaagccatcg tcgatttcct atttttcagt cgcttttgac gccctttgta
     6541 tcataaatag aattggagag gtcatggctg atgacaagct aagagcgact cctgcagcta
     6601 gaaagttagc ggatgattta gggataaacc tctacgacgt ttctggctca ggtgcaaacg
     6661 gtcgtgtcca caaagaagac gtggaaactt ataaagacac aaacgtggtt cgcatttcgc
     6721 cacttgcaaa acgaattgcc ctcgaacata acattgcttg gcaggaaatc caaggaaccg
     6781 gtcatcgtgg taaaatcatg aagaaggatg ttttggccct gcttcctgaa aatatcgaaa
     6841 acgatagcat caaatctcct gctcagattg aaaaagtgga agaagtccct gataacgtaa
     6901 caccatatgg tgaaatcgag cgtattccaa tgacaccaat gcgtaaggtt attgcccaac
     6961 gcatggttga atcttacttg actgcgccaa ccttcaccct caactatgaa gttgatatga
     7021 ctgaaatgtt ggctcttcgt aagaaggttc ttgagccaat catggaagca actgggaaga
     7081 agactactgt aacagacctt ctttcgcttg cagttgttaa gactcttatg aaacacccat
     7141 acatcaacgc ttcattgaca gaagatggca agactattat cactcacaac tatgtcaatc
     7201 ttgccatggc agttgggatg gataatggat tgatgacacc tgttgtttac aatgctgaga
     7261 agatgagtct ttcagaactg gtggtagcct ttaaggatgt tattggccgt accttggatg
     7321 gtaaattggc tccaagtgag ttacaaaatt caacattcac aatcagtaat ttgggaatgt
     7381 ttggtgttca gtcctttggt ccgattatta accaacccaa ctcagctatc cttggtgtca
     7441 gttcgacaat cgagaagcca gttgtcgtca atggtgaaat tgtgattcgc ccaatcatga
     7501 gtttaggatt aaccattgac caccgtgtcg tagatggtat ggctggtgct aagtttatga
     7561 aggacttgaa agagttgatt gaaacgccaa tctcaatgtt aatttaagag caagagtatt
     7621 catttttaag atatagataa aggaaggaag acatggcctt agaaataatt atgccaaaag
     7681 ccggcgtgga tatgacagaa ggacaaatcg tccaatggaa taaaaaagtc ggagaatttg
     7741 taaaagaagg agaaatcctt ttggaaatca tgactgataa agtcagcatg gaattggaag
     7801 ccgaagaaga tgggtacttg attgccattc tcaaaggaga tggtgaaact gtccctgtaa
     7861 cggaagttat cggttacctt ggtgaagaaa gggaaaacat cccaacagct ggagcagcat
     7921 cgccagaagc tagccctgta cctgtagcaa gtacctcaaa cgatgatggt aagagcgatg
     7981 atgcttttga tatcgttgtg attggtggag gtcctgctgg ttatgttgca gccattaaag
     8041 ctgcccaact cggcggtaag gttgcccttg ttgagaaatc tgaacttggt ggaacctgct
     8101 taaaccgtgg atgtattcca accaagacct accttcatag cgctgaaatt attgaaaata
     8161 tcggtcatgc tgcaaatcgt ggtatcgtga ttgaaaatcc taacttcact gttgatatgg
     8221 aaaaactttt agaaactaaa tctaaagttg ttaatactct tgttggtggt gttgcaggac
     8281 ttcttcgtag ctacggagtt gctgttcata aaggaattgg tacaatcact aaagacaaga
     8341 acgtcttggt aaatggttct gaattgcttg aaaccaagaa aatcattctt gctggtggtt
     8401 caaaagtcag caagatcaac gtccctggta tggaatctcc acttgtcatg actagtgatg
     8461 acattcttga aatgaacgaa gtgccagaaa gccttgttat catcggtggt ggagttgtcg
     8521 gtatcgaact cggtcaggcc ttcatgacat ttggttcaaa agtgactgtt atcgaaatga
     8581 tggaccgtat agttccagct atggatgtgg aagtttctaa gaaccttcgc ttgatccttg
     8641 agcgtaaagg aatgaccatc ttgactggta ctaaactgca agaaatcatt gaggaaaatg
     8701 gtcaacttcg tatcaaggtt gaaggaaaag acgatatcat cgcaagcaaa gctcttcttt
     8761 caattggtcg tatgccagac cttgaaggta ttggagaggt tgagtttgaa ttggatcgtg
     8821 gttgtatcaa ggtcaacgaa tacatggaaa cttcagttcc aggcatttat gcaccaggtg
     8881 acatcaacgg tactaagatg ttggctcacg cagctttccg catgggtgaa gtttccgctg
     8941 aaaatgccct taaaggaaat catgcagttg ccaaattgaa tttgactcct gcagccatct
     9001 acactcttcc tgaagtagca gcagtaggtt tgacagaaga acaagcccgt gagaaatacg
     9061 atgttgccat cggtaagttt aactttgctg ctaacggtcg tgctattgca tctgacgcag
     9121 ctcaaggttt cgtaaaagtt atcgctgata agaaatacgg agaaatcctt ggtgtacaca
     9181 ttattggtcc tgcagccgca gaattaataa acgaagcatc aagcatcatc gaaatggaaa
     9241 tcactgttga ggaaatgctg aagaccatcc acgggcaccc aacttactct gaagtgatgt
     9301 acgaagcgtt tgcagatgtt ctaggaatgg ccatccattc acctaagaaa aaataaaacg
     9361 aagatagatt agactggaag gttatttcca gtttctatca tataaagtaa agggatatca
     9421 tgaaatacat tatcaatcat tcaaatgaca ctgcttttaa tattgccttg gaagaatacg
     9481 cctttaaaca ccttttggat gaggatcaaa tcttcctact ttggattaac aagccatcta
     9541 tcattgttgg tcgtcaccag aacactattg aagaaatcaa ccgtgattat gttcgcgaaa
     9601 atggtattga ggtagttcgc cgtattagtg gtggtggagc tgtttaccac gatttaaata
     9661 acctcaacta cacgatcatc tccaaagaag atgaaaataa ggcatttgac ttcaagagct
     9721 tctcaactcc ggttatcaat accttggctc aactcggggt taaagctgag ttcacaggtc
     9781 gtaatgacct tgagattgat ggtaagaagt tctgtggcaa tgcccaagcc tatatcaacg
     9841 gccgtatcat gcaccacggt tgcttgctct ttgacgttga tttgtcagtc ctcgcaaatg
     9901 cccttaaggt ttcaaaagat aaatttgaat caaaaggtgt gaaatccgtc cgtgcccgtg
     9961 taaccaatat tatcaatgaa ttaccaaaaa aaatcacagt cgaaaaattc cgtgatttac
    10021 tcttggaata catgaaaaaa gagtacccag agatgactga atacgtcttt tcagaagaag
    10081 aattggccga aatcaatcgc atcaaggata ctaagtttgg aacttgggac tggaactatg
    10141 gtaaatcacc tgaatttaac gtccgtcgtg gaaccaaatt cactagtggt aaggttgaag
    10201 tctttgctaa cgttactgaa tcaaaaatcc aagacatcaa gatttatggt gacttctttg
    10261 gtattgaaga cgttgctgca gtagaagctg tccttcgtgg ggtgaaatac gaacgcgaag
    10321 atgttcttaa ggcgctaaaa accattgata tcacacgcta cttcgctggt attagccgtg
    10381 aagaaatcgc tgaagcggta gttggataaa tcaaaaagaa gttggccaac taaccaactt
    10441 ctttttttga tatttattta cataatttaa aatacgtaaa atcataaact atccagagca
    10501 ttcttttgtt catcactaac aatatgggta tagaggtcag tgacttgtgt gctagcatgt
    10561 cctagttggt gactgactaa aacttgtgat ttagtcgcat catagagcct agttgctagt
    10621 gtatggcgca gtttatgggg tgttacacgc actttaaaat cctctgagta tttagcaacc
    10681 attttctcaa cgctagaagc atcgatacga ttaggaacac ctctgtagag agttaaaaaa
    10741 agggctgtat ctgttttttc cgttttatag cgttgattcc gaatggccag ataattctct
    10801 aaataaggtt tagcaaaagc agcgacattg actgagtcac gtttgccacc ttttcgagta
    10861 acatcaataa ccatcatttt tagattgaga tctcttagat ctagattaac agcttcagat
    10921 aagcgaacac cagatgccaa gagaagggca ataatggcta aatctcgttc tttatttttg
    10981 ttgaatgatg agagagctcg atttgaaagc tgttgtgggt gctcttgatc gatataagtt
    11041 agaaaacctt ctgtttcatc acctagaaag agtttttgct tgatattttc agctctggca
    11101 gcaagggttt ctttcttttt cttggttgaa acttttttca ttacattacg atagaaataa
    11161 ggttccccct gatcgttttc aacctcctcg gttagatact tgtaaagact agaaagtgct
    11221 gataaggttc gattgatagt tgtctgtgaa acaccttgtt ttgttgtatt agcattcagc
    11281 aagggacgtt cacgtagata aaggataaag gattccatgt ctttcttaga catattttcc
    11341 aaaactgata aaggaatatc ggatatttta tcagcgtttg aaataccaga ctccaaaacc
    11401 cagctgaaaa atcggtcata ttccttaagg tattcgtaca gggttgtaaa actgtagggc
    11461 acagccagtt tagattggta gtattccaga acataccagg gcatgagttg ttttagtttg
    11521 tcgattcgtt ccagtaaaat ctcacgtttc atgtattttc tccataatta agtatactag
    11581 tagtatagca tagacttgta catttttcaa gaaaattata gtttttcaga aagatgttat
    11641 aggagttttt caaagtgata aaaagactta gacctacaaa caaaacagaa taaggaaaat
    11701 aataaaaatc tcacccacta aacttctgtt aatactacat agctagaaat tggtttgatt
    11761 gtcctgattg ttttatccta tttctatttc atttcactat ataattgcta ttgttttctt
    11821 tttcatgatt ttaatcttac tgtacagatt tgatacagta aactcctttc tagctttata
    11881 ctttatcatt tctaacttaa ttatagtctt atttttaata aagttcaatt atttatatgt
    11941 aaaattcctc atcagtaaaa aataaccgat ctcatttctg agaatcggtt ttctaaataa
    12001 atcaagccga ataaatcaag ccgatcattt acataacata aattatgtaa atgatcgtac
    12061 tacaacaaca aatctttgac ttttccaatt tcactttttg gaataaccag gtgaatcata
    12121 tcacccagat acattctggt tgagccgtta actgtttggc tcttgccatt atggacttga
    12181 gttgtgatga ggacgttgtg tggtaagttg agttcatgaa cttgtttccc agcaatttta
    12241 tcagaaactg gtatttcgat aagtgtaact tctccttcgc tagatacttc ttctggaagc
    12301 attttttcca gcatggcttc atagactggc gtacctttga gcaaatccat gataatataa
    12361 gaaacaagag tgacaagacc aagtggcatg aggttgcgaa tatctcctac catctcagtt
    12421 acgaggatca tagcagttaa gggagctttt gatatggctc caaaatagcc actcattcct
    12481 agaatgacaa atatagggaa ttgctcttga ctgacaagtc caagattgac acagataaca
    12541 ccaactaagg caccaagcaa agaaccaaga gctaaaatgg ggaggaaaat tcctcctggc
    12601 agtccacttc catagctaat catactccaa ataaagcgga ttaaaaagta agctaataaa
    12661 acttggaaac taaaattttg ttcagttaaa gaaaggacaa gctgatttcc gccaccaatg
    12721 atttgaggta agaagattcc gactggtatg ataaggataa aagccaagat gggataataa
    12781 gccctatcca aatggatttt ttgaccaatc aagtcataaa ctcttccaac gtttaataca
    12841 gctttctcat agagaaaacc tgaaaatcca aggaaaattc ccatgacgag atatatccaa
    12901 tactgatcta gggtcatggg aggaatgtta tctggcatat ccaatactgg tgtcaaaccg
    12961 aacatgagta gagacacaaa gtttgctacg atgctggctg ctagagttga gacccagaaa
    13021 aagcgcgaaa aatggtgata gacttcttct acaacaaaga gaagtgctgc aataggagca
    13081 ttaaaggctg cggctaaacc tgctgcagct ccactggcaa tcaaggaacg ttcctctact
    13141 ggactggatt tgagccactt ggcaattcct ttaccaccaa ctgctccaag ttgaatgctg
    13201 ggtccctctc gacccagcat gagtccactg gcaatagcaa gaatacctag cacatatttt
    13261 ttccaaagaa tgccccacca gttgagggac atgaggcctt tcagttcggc ttcgacttga
    13321 ggaattcctg agcctttaat atctttttct gaccgtgtta gtttggcact gagccaacag
    13381 atgagtatat aaaacaaaac cagtacaaaa agattgcgca ctaagtaccc ttgatcttga
    13441 taaactcctt gtatcaggtg gaagcccttt tcaattaaga aacgaaagga tccgacgata
    13501 attccaacga tgaggccgac aatgattcct cgaccaactt gggatagtat agtgctggat
    13561 gcaaaggcga attctttctt agaacggact atttccgact gttcctccat ggtatttcct
    13621 ttcaattaat tttctttttt acctaaaacc agtgatttaa cgggttcaaa gctggttcgg
    13681 tgaattgggg taactcctag ttttgtgagg ccttccagat gtttagctgt gccatatcct
    13741 gcattagtag cgaaatcata gccagggaac tgctgatcgt attccttcat caattcatca
    13801 cgtgttacct tggctactat agatgctgct gcgatagaga gggagttggc atctcctttg
    13861 ataatggagg tttgtgaaat gggcaagtcc agtttcatgg catcaatcaa aaggtgctct
    13921 ggttgagggc tgagctggga gattgcttct tgcatggcta gtttggttgc ttcatagatg
    13981 ttgacttggt cgatgacctg attatctatg ataccaattc caatcgacaa ggcttggtct
    14041 tgaacggctt ggaaaatctc cagatgtttc tttttaggaa ttttcttgct gtcgttgaga
    14101 cctttaatct tacaattttt aggtaaaatg acggccgcag cgactacagg accagcaaga
    14161 ggaccacgac caacctcatc aatacctaca attaaggtca atccttgctt ataaagttct
    14221 ttttcataag aaagcatgga ttccaagcgc aaattttcat ctaattcagc ttgaatggct
    14281 cttttacgct tgctgatttc cttttgaact cctgagcgat tatccttttc aagctctaaa
    14341 aaaatagggc tttctaactc cttgactgtc acaaggagtt ctttgatttc tttaatcgtt
    14401 gccatcgagg tcttccaatg tatctaaggt atagttaccg agtttgccat cacggacttc
    14461 cttcacgaag agactgtaaa aacggtcata gtcatcacgg aaaccgaggg cgcgggtcat
    14521 atccataata atcacaggcg cttcttcttc aattttcatt tgtttgaagc gttcagccag
    14581 cttttctgga taatgttctt tgaaataatt gataccaaaa atggtaacct catccatagg
    14641 aagcaactga tccttgatag ctccagtcaa tgccaactta agtgcaacag tttcatcctc
    14701 aaacttaggc cagagaatcc ccggtgtatc caagatttcc agatctttat tggttttaag
    14761 ccattgttga ccttttgtga cccctggctt gtttccaaca acagcaatct ttttaccagc
    14821 caaacggttc atcagagtgg atttaccagc gtttggaatc ccgataatca tggtacgcaa
    14881 ggtttcaatc tgaatcccac gttctttctg gcgagcaatc ttatcagcca tgagcttctt
    14941 ggccgcatct gttacaactt ttacagtcac ttgctctttg gagttgatag ctagcgtctg
    15001 gatttcttgt gattcaaaat actgacacca ttccttggtc attgctggat cagccaagtc
    15061 ggccttgttt aaaatcaaga gttttggttt atcaccaaca atcttggtca acataggatt
    15121 ttgactagat agaggcaagc gtgcatctac taaaatcgtc acaaaatcaa caaattttaa
    15181 attttcctgc acctgtcgac gcgctttaga catgtgacca ggaaaccatt gaatagtagc
    15241 cattgagttt tttcctttcg tagctctgaa gctgttaatc tagaactaag agatctcaat
    15301 ctaaaaatga atgatgtcca aacagttctt gttttatgat ataaaaatct actttatatt
    15361 atatcatgcc ttctttattt tgtccgatga tagcctttta catattatat catgccttct
    15421 ttattttgtc cgatgatagc cttttaccct atcttttacc ctatatagaa aaaagccatc
    15481 aaatgatgac ttttcagagg gagcaaaaaa ctgtcctctt tatattttaa acatttaaat
    15541 gtcttcttta aacagtttat taaagaaacc gtctgtgttt ttaagaaggg ctttgaatac
    15601 tttggatttc aagtctactg tatttttgaa atgatcttta attttcggat ctgacatacc
    15661 atcaatatct ttttgaacag cttcagcaaa taaagttttc aactgagcat aatttgtaat
    15721 ttgacgacca tcaatttcaa tagcagtaaa tcctttttct gcttttgact taacatcttt
    15781 gaagtattgt ttcttccact cttcaagatt attgaattta tccttagata ctttcttgat
    15841 aatgaaatca tcacccaata attttttatt ggttttgtca gcttcgcctt gatatttgct
    15901 agatgcataa ctaatgaatc cattttcata accaaaatag ccccacatac ggaaagtatt
    15961 atgcttgaat gatgcagctc caggtgctcc ttggctagta ttaccaccat agataccggt
    16021 cattatatta atatttacgt aagcactatc aaaatcttct ggattatatg ttctgtcact
    16081 aggagcgcca tgttttgtca tcaaattatt gttgactaaa tcatcaatgt tgttgataac
    16141 aattttcttc ttctcatctt cagttaattc acgaacttta tcaaattgat gtagctctcc
    16201 atttttgcga tattgtctat ccattttctt gaaccatcga ctaatattac catgtaattt
    16261 agggataaca gcatcagcct caagatggtc taacatcata agagcatcat tataatttct
    16321 catataacgg tcgatatctt cacgagtttt caatttatct ggatcataat tataccattg
    16381 gtcaccgtca ttttcacgat gataagccat gttaagacca agagcaccat attcaccgtt
    16441 accagattta tcaggtgtct gcaacatacc ttgcgcatag gcttcaacgt ctgtaccttg
    16501 acggtgtcta tgcccaccga agtaaagcat tctatcgttt acgtgtgttg tttcgtgagt
    16561 aaaggctgaa ataccaaacg gactaatcat atttgttccc atgaaataaa cttggtcatt
    16621 attattggga tcttggaaaa tagatgccat agctcccatg taccaatttg tagtatgata
    16681 acgccaagta ggtccatata attcacgaat aggtgcaatt ggtttattat tcttatcata
    16741 acccatacga tttacacctt cgacgttatg atagttttgg ttatcccaaa caggtgttgg
    16801 aaccctattt tgactcttga gtaattggtc acgtacgctt ggcaatgcta aacgcgacca
    16861 gaagtcaaga taagtttgtt gtgcgtgagc aacttcttta attttatctt taaatgcttc
    16921 tcgttcagct tctgtatact taccatattt ttcaaaagag ctataagcca ttgtattgta
    16981 agtcgaaatc aagaacatat gtgcattctt aagagttaat agaggtagga tcatacgatt
    17041 atgaacacca ttattcaaac catcaaatgc acgatgtcgt ttattaacaa aatctgggtt
    17101 cgttgtttta ggctccacaa tgtatacgtt cttagccgca tggataaacc aatcatttaa
    17161 atcagtatcg ttagtgaaga gacgcatgtt gtaatctaag aagccatgta attcagactt
    17221 accaatagtt ccaccaatca tatcacgata agcatccaaa gttctgtcac ccttgatatt
    17281 tcgctcactt gatccaattc gaatcaagaa gtcaagaagg tcaactgact taccatagaa
    17341 atcaggttta aacatcatca agtttttgat agtcatatcc ccatacttta tatcatagta
    17401 acgattaagg taagtaagag ccatcacgat tttagcttta ttttcatcca ccttcttaac
    17461 aagtgctttc atagctgctt cgtcactatt taattgatgg tcagcatttt caatcaaaga
    17521 tttgaccaat ttatcaagat tagctttcgt ttcagaaaaa ctttcttcga aataaaggtc
    17581 tcggatataa ccattcttac gctcatctga attagcatta ggatctctag atgtatctcg
    17641 tttatccatc aaagcacgga cttcgggaga aattaattca acagttgcta acctatcttt
    17701 aatgtcttta atcaattgat cacgattttt atcaaccatg tttggtgtat aaacaatgtc
    17761 atctaatcca tcgatactat actcacgaac ttgcgctact ttagactctt tttgatgaac
    17821 agctttgatt tctttagtct tatctgcata gtgaatcatc acatgatcaa tatctgataa
    17881 atcggtcacg aattgaccat ccttcatacc cgtaacagaa aggacttctt tcttagctaa
    17941 gtttgaatct tctgcaagtt tgttaccttg attgacaatc cattctttgt tataaaatgg
    18001 ttgcagtttc tcaatattac gataagccag atctcgatct actttatagt cttgggttga
    18061 cttatattca tcttctcttc tggttaaacg atttaagcga ttgaccacag gttcattgat
    18121 ttcgtattta tctgcggtaa ttcctgtagc ttcaatttta gattttgcct cttcctcaga
    18181 aataggtctg atacgatttt tatcttttga acgctggtag ctagaatgcc catcactaac
    18241 acctattaca acataatttc gtcttacatt atctcctgtc cagtagccat catcataatc
    18301 aatatcacta gaaccataga aaatttcacc attaggtacc ttcatcatgg aaactgaatc
    18361 ttcgacagaa ccgtggtgct tcatgcttcc tacaacacca ccactttcaa tcgcctcagt
    18421 tacttgaatt gttcccttag caatagattt tttgattgct ccttcctgac gaacagtatt
    18481 gttatttcct tcattttgtg tccaataagc aatgcccgca gccttggctt tcttgccaat
    18541 aagatttgcg ttaacatacg cgcgttcgac gagtcctttc cagttctcac caacgatacc
    18601 agtcaagaat tgtccaccat cgccgacaga agttacgtta ccgataaagg ccacatttct
    18661 gagtgtacct tggttatcaa tcttatcaat aaatccagat acccaatctt tacctactat
    18721 attaccttta accttaacat tttcaatggt agttccacct ttaataatat tcgctacagc
    18781 tgcaacttgg ttcgcccatg gcatatttat gtcaacatta ccaagattaa tattcttaat
    18841 cgttccacca ataacattgc caaataacgg acgtgacaag ttatgaatgg tatagtgatt
    18901 atcaccatca ctcaagagct tacctttgaa atctttcgta acatatgatt taccaaatgc
    18961 tgatacattt gtggcattaa ggtcagcacc caatttaaac tcgtcatttg gatgttcttg
    19021 catatctctc acaagttcat tgaaattgta atatacatta ttgacttttg gaactggttt
    19081 ttcaatgtaa tgaatgtatt catttctagt cttagatgga tcgacatgtt gaattaaatc
    19141 ttgagcgctt gctgtaattt tataaagttc tttttctttt tcagttacta actcaatctt
    19201 atcaactgct aaacgagtta ctttattatc acgactagtg atttttaagt acattttctt
    19261 cacatcttca tcagaaggtt tttcgctcat gaaatcacta ggtatttcag taccatcatc
    19321 atttaccttc actaaattag tagatgaaat atttttaatt tcgacctttt tcaactccaa
    19381 acgcaaagtt ttttcatcca gtttagacgt ttcagaacct tgaccacgat cataaatcat
    19441 tgttgttgca atcttataat ctttaaacaa atccaaattt tgtacagcgt cagttaattt
    19501 ttctttagat aaatcaacct ttttaactac tgtatcacca tctttaattt cagctataat
    19561 acttgatatt tcagcattat ctttattatc aagctcataa tttaagttgg cagaacgatt
    19621 catagcatct tctgttactt tggtcaaggt caaaacggga actgtttttt cttttggatt
    19681 ttcttttgta ccttttacaa gaattttagg tttcatttct ttcaaaattg ttaccgtttc
    19741 aactggttca ctaatttttt tgccatgtaa ctcttcatag ctagtaacaa tttgacgttc
    19801 gccatcctca ccaacttctt taacagaatc atcgcctata aatttgctag gatcagattc
    19861 ttctatagtg gttttaggaa tagtctctgt acgaatatcg gtatgtaaag aaggcaactc
    19921 ttctctttct gatgcaactt ctgaaactgt agatattggt tcagtatact ctggaatgtc
    19981 gttaacaggt ggttcaatca agtttccatt ttcatccact cctgttgtac ctactggttc
    20041 agtgtatgct agtttctcat gaacttcagg ctcaacaagg tttgcaccta ttggttgcgt
    20101 atagtcaggc ttctcatgaa cttctggttc gcctttgtca gtcacaacag cttctggtaa
    20161 tgtcggttgt acttccggtt cgcctttgtc agtcacaaca gcttcgggta aggctggttg
    20221 aacctccggt tcccctttct ctgttacaac tttgggttgc tcaactggcg aaacagttga
    20281 tgctggagaa accgacgaaa cagttttatc atcaagctcc aagtaaccaa catatctgta
    20341 accagaaatt tgcaacacac catcacgtcc agactcatgg atgttagcat tcaaattaag
    20401 tgctgaagca gttgatagtg taactaaacc tgtcgcccct acaatcaaaa atgtagctat
    20461 ttttttattt ctattatctt ttgtgataat cacaataaga ctcccgatag ctaacaaacc
    20521 caatgcagtc ataatagatt gagaactgcc tgtgtttggt agtgcgtctt tttcataatc
    20581 caaagcatat gactctttgg attcatcagg tctaccttgt ttgagttgat cacgttcagt
    20641 ttgtgtcaaa ctactataat ctaagtaatg ataagtcgat gcaccaataa ctgacggtgc
    20701 aaataaaaga ctccccaaga ataccgaacc aacaataccc tttattttcc gaatggaaaa
    20761 tttatctttt tttaataatg acaattctaa tcctccacat tcacttttta tttcctaata
    20821 gtaaacacta ttttttattc tcaaaaattg aacttctaaa atcatttgtt tggtcaagat
    20881 aagctttata tattgctttt ttcagtttaa gaactctact atttgtttct gggtaaaatt
    20941 ttgctacgtc atcttgcgca tcatctctca ctgcttcgac aatcattctc tctagtacac
    21001 ctatatccgt aaccgataca tttcgttgac gactcaatgg attattagga ttaataaagt
    21061 taactctatt tagtttactg aattgttttt ctctttcctt atacatatcc ttcttgaatt
    21121 gtgtccaagt tttatattga ccattaaata ctttttcaag tactaattca tctgtaacta
    21181 aacctacttc tttaccataa gaattaatct tacttcccgc cgcttttgca tctttttcat
    21241 attgatttga gatataaggt accatgccct ctttatatcc ttttgcagct aataattcga
    21301 atgctatacg gcgtcccatt aaatcaccag gagtcccttt ctctccacta agagctgaat
    21361 aaatcggtga aaataaatca attgtataat aaccatttct cttatattct tggtctttgt
    21421 accctctgct agtaataata tcattttcaa ttaaactatc aaaagaattt aatttattaa
    21481 cttcatccat cgttaatctg cgaacaacat ttgtcgcgta tacattatta ccgtcttgat
    21541 caagatcaaa tttattttca atttttctta gtaactcata tttttgattt gtttctaaat
    21601 tagctacagc ttttccttct aaatattcta acatataaat tacatcaaac atattatgca
    21661 tataattatg taggtcttct gcactattaa atcgttgagt tggatcagct acttgaagtc
    21721 ttgttgaatt ttctgaatcc tcatatttca agacagagtt aattgttatt gttggatcat
    21781 atggatgatc cggagcttgt aataatcctt ttgcataaaa ctcaggtcct aatccgcttc
    21841 ttcttccata tcctccaaga taaatctctc tatctgagtt atgtgtcatt tcatgagtat
    21901 atgttacagc tccatcttta tccaacatac gatatgccat ataatagaat gcatctccgg
    21961 tagcatatgc tccgtgtcca ttatgaacaa cattatttcc cgcaggtcca aagaagtatt
    22021 ttatagctgg atgatcagtt tcaaaagtag cttgatgagt tactttgtct ttatctttgt
    22081 cagtaccaaa tttgaaagca tcatatacta gaatacttct atataatttt tctttattat
    22141 cgttatctaa tatcttatac caataatcat aatgatctct aaatcgttta gccgcttctt
    22201 tggcgttatc ttctacaaat ttgtttaact tttctccctt aggatattct gaacttctat
    22261 atctgtcata ggctccaaat ccaatagtag aaatattcgc tatcataaac actgtttttt
    22321 cttcaggtaa agttaatagt ggtaagacca tactttgata tttccaagat ggattagtta
    22381 atctatcata taccccaata gaatatttag ttccctcttt tccttgtttt tctcttccct
    22441 cttcaattgt agatttttct tctacaatat atgcttttgt ttgtttttta aaccactcat
    22501 tgttagaaat tgttggtaag aatacttttc tatatccttc taatgcttta aataaacttt
    22561 cagttccata atttttcgct aataatacat tataagtaat
//
LOCUS       DAVKMU010000033        21334 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKMU010000033 DAVKMU010000000
VERSION     DAVKMU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.033"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(411..2138)
                     /gene="ezrA"
                     /locus_tag="VW343_001436"
     CDS             complement(411..2138)
                     /gene="ezrA"
                     /locus_tag="VW343_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9755975.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPPQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2220..4166)
                     /gene="gyrB"
                     /locus_tag="VW343_001437"
     CDS             complement(2220..4166)
                     /gene="gyrB"
                     /locus_tag="VW343_001437"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9755976.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVYVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4181..4744)
                     /locus_tag="VW343_001438"
     CDS             complement(4181..4744)
                     /locus_tag="VW343_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9755977.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4829..5383)
                     /locus_tag="VW343_001439"
     CDS             complement(4829..5383)
                     /locus_tag="VW343_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9755978.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5394..6617)
                     /locus_tag="VW343_001440"
     CDS             complement(5394..6617)
                     /locus_tag="VW343_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9755979.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6654..9104)
                     /locus_tag="VW343_001441"
     CDS             complement(6654..9104)
                     /locus_tag="VW343_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9755980.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVVLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9304..10233)
                     /locus_tag="VW343_001442"
     CDS             complement(9304..10233)
                     /locus_tag="VW343_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9755981.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10373..10660
                     /locus_tag="VW343_001443"
     CDS             10373..10660
                     /locus_tag="VW343_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9755982.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10694..12028)
                     /gene="ciaH"
                     /locus_tag="VW343_001444"
     CDS             complement(10694..12028)
                     /gene="ciaH"
                     /locus_tag="VW343_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9755983.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12018..12692)
                     /gene="ciaR"
                     /locus_tag="VW343_001445"
     CDS             complement(12018..12692)
                     /gene="ciaR"
                     /locus_tag="VW343_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9755984.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12801..15347)
                     /locus_tag="VW343_001446"
     CDS             complement(12801..15347)
                     /locus_tag="VW343_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9755985.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15470..15910)
                     /locus_tag="VW343_001447"
     CDS             complement(15470..15910)
                     /locus_tag="VW343_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9755986.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQAHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15885..16754)
                     /locus_tag="VW343_001448"
     CDS             complement(15885..16754)
                     /locus_tag="VW343_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9755987.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPSVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16796..17524)
                     /locus_tag="VW343_001449"
     CDS             complement(16796..17524)
                     /locus_tag="VW343_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755988.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17532..18011)
                     /locus_tag="VW343_001450"
     CDS             complement(17532..18011)
                     /locus_tag="VW343_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9755989.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            complement(18022..18720)
                     /locus_tag="VW343_001451"
     CDS             complement(18022..18720)
                     /locus_tag="VW343_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9755990.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18713..18979)
                     /locus_tag="VW343_001452"
     CDS             complement(18713..18979)
                     /locus_tag="VW343_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9755991.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(19045..19971)
                     /locus_tag="VW343_001453"
     CDS             complement(19045..19971)
                     /locus_tag="VW343_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9755992.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20046..20861)
                     /locus_tag="VW343_001454"
                     /pseudo
     CDS             complement(20046..20861)
                     /locus_tag="VW343_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20865..21185)
                     /locus_tag="VW343_001455"
     CDS             complement(20865..21185)
                     /locus_tag="VW343_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9755993.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6458 a         4678 c         3737 g         6461 t
ORIGIN      
        1 taccgtggac taaagttgta caagaaaaat gcaaataaga aatctccaga ttaggaacta
       61 tccgtgagtt ctctagtctg gagatttttc aatagacttc gttattggac ggttaccaag
      121 ttactcaaag tacagatttg aagctgcaga taagactgac gaaatcactt taatactaaa
      181 tgaaaatcaa aaagcaaact aagaagctag cctcaggctg tacttgagta cggtaaggcg
      241 acgctgacgt gatttgaaga gattttcgaa gagtataaaa cactgttttg aggtggtgga
      301 taaatcggct gggtaaaaac tcaatctcag gagaaaatga agtaaatctc cccacaacta
      361 tagaaaaaga tttgattctg ctcctcacac aataaaatct ttttctttta ttaaaaacga
      421 atcgtttcac gtgttttctc atatgaggta acaaagcgat tggttacacc aggctctgcc
      481 acttccaatg cttgagaaat cttatcaaat gaagcgtgat aatcaaattc tttttcaaaa
      541 atatctaaag cttcgttaaa tgcttcttga atgcgttcat caaatgagcg atagcggtta
      601 gaatattgca agagttgctc tgtcaaagtt gcatattgta caatattata agtttccgtt
      661 tctaaagctt ccatatcatt cgttgcaatt tcaagaactc gggtaacaga ttcaatgtta
      721 atcatttttt gttctaactc aaccattaaa tcctcggtat tattgcttgc cgtaaagaat
      781 aacttcaaga aagtttgtgg aatacctggc agattgcgtt tttccatgta tcgcttgata
      841 gtatggagac gattgacata aacattggcc ttttgacgtg cattaatatc atctttctca
      901 atttgtgtca ggcgctcact aactgaaatt tgctcatctt caatatcttt tagttgagtt
      961 tgtaaatcct caagattttc ttcaagaact gaataagctt ggggtggttc ttcttgattt
     1021 gaagttacct caacaatagc tgcctcaaaa ctctctaatt ctgtctgaat acgacgaaca
     1081 tggcttgcag ctgtctcagg aagtaaatag gtcttgttca aacgtgcaat atcttctccc
     1141 aataaagtat tattctcttt catatgttga agataagttg gaagagttgc aagtagattt
     1201 tccactactt tctgagcagc aatttctcga gtaaaaatat catacaaggc attgatttcc
     1261 tcttgtgcct gtccattctc atattcggca ttatccaatt ccaactgacg aatattctct
     1321 tggtttttct tgaatgcttc ataaagcaag tggaaacgcg cttcaatatc cgtttcaaca
     1381 aaatgataat tagcatcaat tagtttacga taaccggctt ccaaatcctg taattgatct
     1441 ggcaattctg tagaaagcgt cgtaaccaag gctggaacac gatccacaat atgacttaag
     1501 gccaaaatgt gattttctgt attatccaaa atcactgcgg cttccacagg gtcacccgat
     1561 gaattcaagg ttacaaattg tgaaaattca gattggatat tttctaattg tttttcaatt
     1621 tcatccaagg cttgaccata ctgttctgaa ttttcagcaa ctctatgctg aagttcctca
     1681 aataaatcca aagcatgaag aacacgacca ctatttttag attcttgctt ctctaagtct
     1741 gccaaagcat tgcgaattgc cgcaatatct tcttcaatca aagtaatttg actctcaatt
     1801 tggtcaattt gatgactggc cttgagaaaa cgaaatgaat ggttataacc ttctgcttca
     1861 aagagattat tttcaatatc ggcaaaagag ttgagagata aatcgaccca tttttgattc
     1921 cattcacgga aagccacttg actttgtcca atcaagtgca tattttttac agcttctact
     1981 tcatcattta ctggaagatt gtatagttct tcttttcttt cttctagtgc ctctaatctc
     2041 ccctcgtttc gcttacgtag aaagattgcc actacataag ccagaactaa aatgactgca
     2101 attgcaacca ttaaataaat tagttgtcca ttagacatat caaactcctt ttttacttga
     2161 aacaatcgta acgattatat catatttttt agaccaaggg aactacttct ccctattttt
     2221 tagacatcaa gtgtactata gacagcattt tcttcgataa actcacgacg aggctctact
     2281 cgatccccca tcaacatatc aaagatttta tctgcttctg cagcatcatc tacagaaact
     2341 ctagccatca agcgatgttc gggatccatg gttgtttccc acagctgatg atcgtccatt
     2401 tcacctagcc ccttataacg ctgaatagtc ggtttggtac gaccttcact ataacgggct
     2461 aaagcttctt ggagtttgat ttcttgatct gcacccggct ggatatattc tttaatctcg
     2521 cttccaacct tgacaccata gattggtggt tgggcaatat aaacataacc agcttctagg
     2581 attggtttca tataacgata aatcaaggtt aaaagaaggg tacgaatatg ggctccatcg
     2641 acatcggcat cggtcatcaa aacgagtttt tggtaacggg ctttcgaaac atcaaattct
     2701 gcgccaaatc ctgttcccat ggctgtgaaa agactacgaa tttcttcgtt agctagaatc
     2761 ttatccatac ttgctttttc aacgttcaaa atcttaccgc gaattggaag gatagcctga
     2821 aactcacggt tacgaccaga tttggctgat ccaccagctg agtctccttc gacgatgaag
     2881 agttctgttt cagcagggtt attagaagaa cagtctgcta gtttccctgg aaggttggaa
     2941 atttccaaac cagatttttt acgtgtgact tcacgcgcac gcttggcagc cacacgagcc
     3001 ttggcagcta aaatcccttt ttccacgata cgtttggcaa tctgtggatt ttccatgagg
     3061 aaatcggaga aggcttcact gaagaggcga ttggtaatct tgaccacttc gctatttccc
     3121 aatttggtct tggtttgtcc ttcaaactgt ggatttgggt gtttaactga gataactgca
     3181 gttaatcctt cgcgaacatc ttcccctgtt aaattatctt cattgtcttt cagtaactta
     3241 ttcttacgag cataatcatt gataacacga gtcagtgccg tacggaaacc ttgttcatgc
     3301 gttccacctt catgggtatg aatattattg gcgaaactca tgacattttc atggtaacct
     3361 gttgtgtact gcatggctac ttcaactgta atatcatcca tctcaccgtc tgtatagatt
     3421 ggtgtatcaa agattacatc cttgttctcg ttgatatatt caacgtaact agcaatccca
     3481 ccttcataat gataatgctt ggtttgttcc aaaccttggc gcttatcagt gatggaaatt
     3541 tgaagaccgc gatttagaaa ggccaactct tgaatccgtt tatttaattt atcaaaatca
     3601 aagattgttg tttcagtgaa aatttctggg tccggtgtga agtgaaccgt tgttccagtt
     3661 ctatctgtat ccccaaccac ctcaaggtct gcaacaacat gaccacgacg gtattcttgg
     3721 taatgaatct taccgttttt atggacataa acgtctaatt gagtggaaag ggcattaact
     3781 actgacgacc ccaccccgtg aagaccacct gaaaccttgt atccaccacc gccgaacttt
     3841 cctccagcat gaaggactgt aaagacggtc tcaacagcag gacggcctgt tttttcctga
     3901 atatcgactg ggataccacg cccatcatcc acaacagtaa tcgaatcatc tggctcaata
     3961 aaaacttgaa tatggctggc aaatcctgcc aaggcctcgt caattgagtt atcaacaatt
     4021 tcccagacta gatggtgaag accttctttt gaggttgatc caatgtacat ccctggacgc
     4081 atacgaacag cctctaagcc ctctaaaact tgaatttgac tggcatcata atcctgtgcc
     4141 tgcagatttt tgatttcttc tgtcatctta ttcctttttc ttatatgtct aatacttgtc
     4201 ttaaattcac gatactggta aacaagtggg tatcaagtcc tgcagcttga cctgcttcga
     4261 tatcaatcgg ccgatcacca atgacaagac cagagctaat ctgatacttt tctcttaaat
     4321 aaagcatgga ttcgggattt ggctttctct taaagcctga gctagaagtc accacttctg
     4381 taaaataagc tgctatagag gttttttcta aaatttccaa aacctgatca tttcgatgag
     4441 agaccaaaaa atgacggcca ccttgatttg aaatgacttc caataggtca gaaactcctt
     4501 caaataaaat cgggtgttca agctctctgg cttcattttc cttgtacttt tctaaaaaat
     4561 tctctaaatt gggagcgaat gtctcaatcg caaaaggagt agaaaccttt aaagcttgat
     4621 agacactgtc atggtcttgt gtgataccat acagtgccaa tgtttcaaca aatgcagctg
     4681 ttgaagtttc ataattatcc agtaaagttc cacctaaatc ccagatgtaa tcgtgatatt
     4741 tcatgccctt tattatagca tttttttatg aaaaaagcga tgatttccct gagttttcca
     4801 cataaaaaac gagagaataa ctcccgtttt actgattttt accaaagaca tcttgataga
     4861 gcattcctgt tcgtaaactc tctgcgtccc ctcctagttg ctccaccaac tcgtaggcaa
     4921 aggcaagggc tgttgaagga ccccgactgg ttgtcaactg accatctacc actactgttt
     4981 ccttgacgta gtgaccatca aggatttgct cttgaacgcc gtcataacaa gtgtatcgct
     5041 tatttttcaa tatctctgct tgattgaggg caattggtgc cgcacaaatg gctgctagtt
     5101 tcttcccttc ttgctcgaag ctttgcaatt cttgaatcaa ggtctgatta tcacgtaaat
     5161 gtgcagaacc aggcatacct ccaggaagaa caatcatatc atagtctgat aaatctccat
     5221 caaagacatg atctgctctt acttggattg catgcgaacc cgttacttgc tcttcaaaac
     5281 caaccatatc acatgtgata ttggctcgac gcaagacatc tacaactgtc aaggcttcaa
     5341 tttcttcaaa gccctgagct aacataactg ctacttttac catgattttc tccttattta
     5401 atttgtttta atacaacctt tttccgtttg aatgggactt ttccgctctt ttcttcagct
     5461 aaattagtct ggtaactcat cgataaaagc aaaccaacac caatcaaatt actgataata
     5521 gctgatcccc cttgcgaaat gaaaggcaag ggaatccccg tcaaaggaag tagtccagtc
     5581 acagcaccga tattctcaaa gatgtggaag agcaacatca taatcaaacc tgtggaaata
     5641 taagtgtaga actggttatt tgatttaaga gtaatcttca acatacggta aatcaacatg
     5701 agatagaggg caataaccag gacagagcca ataaagccaa aatcttctgc aataaccgta
     5761 aaaatcatat ctgactctcg aactgggata agcagattcg aagcattaaa tccctgacca
     5821 aataagccac cactcccaat ggcaatctgc ccttgagcct gctggtaagt cgttgtttgg
     5881 gcaaactcaa agggattgag ccaagccaaa atccgattaa tttggtaggt cggcattcca
     5941 atctggtgaa gaaaagctcg tccgtccttg ctaataaaga tagctaagaa accagcaact
     6001 cctgttacag cagtcacaaa tactgggata ataattttcc aagaaacccc tgataataaa
     6061 acgattcctg agaaaatggc tacaaaaacc aaagccgtcc ccaagtcact ttgaagtgct
     6121 aaaagaacta ggactggaat ggtaaagaga atcatccaga aaattaacaa aaagtccagc
     6181 ggaaccgtgc gtctccattc cttatgtttc tttgtaaatt ggacaatgac acgagccaac
     6241 atgaggatat aggatatctt cataaattct gacggttgga atagggtaat tccatttatt
     6301 gatacccagt ttttggcacc cgttgatgca actaagcttg gattataaaa tacaatcggc
     6361 aagatcataa gtcccaagcc taaaatatat agaaaggggg tcaccttcca aagaaattct
     6421 gtattaaaga gcatgaccac aaaaccaatc acaagcccca aggcaatcca ggcgacctgc
     6481 tgccctaaaa tgggcagaat attattggga taatcatgac taacggctat atagatagcc
     6541 accacaccga tgaccagtag aaaaaatact ggcaagagca aactgtaatc gactcttgag
     6601 tcgagagaac gtttcatata tactaacctt atactttcat acaatactat ttatcaaagt
     6661 tcattaaaaa atctatcaat agcctcgtca acttcggatc gagagatggt tttaacagtc
     6721 gcttcttctg ctagagatgc tactatttgt ttgccgtatc gttttccgac gattctccta
     6781 tccaaaataa gagttaagga acgttggtat tcacgtctca tacttcttcc caaagcctgt
     6841 tttaaacgaa taatggccat tggcaattga taatcataaa aggcattttt cccttcttga
     6901 ttcagttctt gattaatctt tttcgtcaag ggttcttgag gattttggaa aggaagcctc
     6961 ggtacaactt gaatcacaaa aggatggctt gaaaaatcaa ctccctccca gaaacttgct
     7021 gcaccaagca agatttgttg ttcacctttt tcaaagcgtt tctttagctg atgaacatcc
     7081 ccatttttat actgggccaa gtggctaact gtaagtaaat ccgatactgc tagaagcatg
     7141 tctttagcgg taaagagaac caaaatcggt tgctggaaag cttgaatttc cactagtaaa
     7201 gcagctacct ctctggcata gacttctaag gaggtttctg ttaccagagg gaaatctttg
     7261 accataacca cttcttgttc ctgttttccc cgagattcaa tcttgacaaa tttggcttca
     7321 ggatagccta aaaggtctgc caaagaaacc ctctgactaa tctcaagagt agccgatact
     7381 cccaagactt ggcaactctc aggaagtaaa gaggacagaa gagtacggcc tgattttgta
     7441 gaagaaatct gaattttctt ttgactcgtt tcagttactt caagccagta atcaccttca
     7501 gctgtaaaat agcgaaccag ctcatcaaag ccttctactt ctaattctga aaaatactga
     7561 tggagattgt ccagagaatc taagatattt ttcctagatt taccagactg aaattgttct
     7621 atcaagtaga gacactcaaa gcgaatactt tctagtatcc gttgttgaac cctgttttct
     7681 tctcctacta aagccttatc aattaaatcg ataatagatt gtatatcgta ggtctcttga
     7741 agcagatttt ctagagctaa caaaatcttt tggacttcat caataatcag taaacggtca
     7801 ctgacaaatt caggattatc ttcaagtctg gtcacgagat aggcatgatt agtcactaaa
     7861 agcttgcaag tctctgccct ttcttgacta cgtttccaaa aatcttccgt cacaaataag
     7921 ctctgggatg ataaattccc atcatgacga aggtctgcta gaaaatgttg gtaacggtag
     7981 agttgcccga tttcatccaa atctcctgtc tctgtctcag taagccagac caagacttgc
     8041 attttaaagc gtctaaataa gcgattttca tcattttcct gcaaggaatg ataaaaggca
     8101 tccaacttca gataattttg tggtcccttt aagctatgaa tatctgtatg gaacacttcc
     8161 ttgagacgtt taccttcttc ttccatgatt tgattttgaa gaatctttgt cggaacacta
     8221 agaacaattt gtcgctcttt ggattgagat aaagcgggta agagatagcc ataggttttc
     8281 ccaatccctg tcggcgcttg aatcagagag acaggttcat ctttcaatag caagacaacc
     8341 tctttagcaa aactttcttg ttcctccctc acttcaaggt tcaacagaga aatattttta
     8401 gaaaagtctt gagatagttt tcgtagctcc agagaagctg ccgttttctt aaaatacaga
     8461 ccttgaactt ggaccaagtc tggagaactc aggatagatt ggttgcgata agtttcctca
     8521 ataaccaggt aggactcata taggagagcg tcagccattt ccagcaagcg ttccaagaga
     8581 cctttaggaa gctgggtcat cttttcccgt aaaaaaagaa gtaattctgc tgtagcttgg
     8641 gcatctgaaa gggctgtgtg tgcgtgttta agaggaattc ctaattctcg acacaaaatc
     8701 ggcaagctat atttttccag ttcagggaaa aagacctggg ccaattcgac cgtatcaaca
     8761 cgagggtttc ttagctcata gccttcaaaa aataaatttt ccgccaagag attagcatca
     8821 aactgaacat tatgggctac aaaaatccca tcctccacca agtcaaatat ttttctggca
     8881 acttgcgaaa aatcaggtgc ttgcgccaga cgttggtctg tcaatcctgt cagttctttg
     8941 atatgagcat ccaagggttc atgtggattg acatccgtcg tatagtgatc gacgatttct
     9001 ccgtcctcaa tcacgacaat tcccacttgg ataattttag ccttactacc tgtgctagtt
     9061 gcctctaaat ccaccacaac atagcgatta ccaatcgttt tcattataaa aaattatacc
     9121 aaaaaaaggg ctatttggca cctgcaaaca taggataatt ttcaaactca agaacactac
     9181 ttttcttaaa aatcccatcg aatagagcat tctagcctgc tgatttttgt aaaatagtga
     9241 agaaatacga aaaattgaga gaagaaatgt tttcatcttc cttctctcaa cgaatcatgt
     9301 attttattta accatgtcag gatcgtagtc ataaaatcct gtatcaagga aacggttttt
     9361 agggtgaagt ttacgaactt cttcatccag caagaaggct tgatcatggc taaagttgcc
     9421 ctcttgaatc aagttacgcg cttggataaa gagttttgca atctcaacaa agttctgacc
     9481 aggagtgtag agcccttcta gtttccagcg agtaaagcca tgctccacca attctgttaa
     9541 tttgatcatc aaatcaaggt cattgttggc aaagatatgg gtcccatgat tatcttcaaa
     9601 aatggaatag tggctctctg gatcacttgg ctcagccaag aagaggtcac gtttatgcgt
     9661 cttttcatca tcgatatgtg taaagttata gtagttttgc aagagtggac gtttagaatg
     9721 atggatgacg ctagcaccgt aaaccaaaac ttcagcagga atttccaaaa tctctggcat
     9781 tttgaaaagt tcagctgatg gaatttcacg cgccaaaaca gcctcagatg caccagcctt
     9841 ttgtccccag aagttaatct gacgactgct agttaccatg gttgaagcat cgtagatggt
     9901 cttaaatgaa taaccatcgc ggttaactac gtaaaagacg cctgcatccc caatcgtaat
     9961 atagtctgtc ttgatttctt ccaagaagtc taagaaaggc ttgatacggt ccatcatatc
    10021 ttggtgcatg agagcattga ccgcaacgag caattcctta ccagcatcat gaaccaactt
    10081 agcgatttca cgtaattggt cataactaaa ggtcgttggc agacgaaggc caaaatcttt
    10141 ctcaccgaca tagatacggt ctacgccagc ttcgagtagt tgttcaactt gttcaatact
    10201 ttcagcagtt gctgtaatga caatcttttc cataagaaaa attataccac atttctccaa
    10261 aatcagccat gctttgaaaa tgaaaaggga ctttattctt tttgtaacct ttctgtaata
    10321 attctctgcc gaaaaaatga taaaataggt atgtactgtt aaggagagaa tcatgcccgt
    10381 aagaaaatta caatcctatg aggtagacta tcaagaagaa ttaaaccagc agcttcctca
    10441 ttaccaagct tatacacctg aagcgcaatc tgatgccaat ctcaaggaaa tcctattttt
    10501 tattaatatc gctgtttttt gtatctgtat tgctatcttt agttttatct ttttagcatt
    10561 aaaattatca actgctcttg cctttgccgc agcaatcgga ttcagcttac ttgttttaaa
    10621 agttcaacgg tctattatca aacgaaaacg tagaagataa atccaaaata tcgccccaat
    10681 tggagcgata tttttatttt ttctttttag atggtgtctg aatggcaatc ttcacttcaa
    10741 agattgttcc cttgggttta ttatctttga cagtaacagt tccttttaga gcatctacaa
    10801 tttgcttggc tagggataat cctaaaccaa aaccaccttt ttgccgggtt ctagccttgt
    10861 ctactcgata aaaacggtca aaaattttct ttttatcttc tgtcgaaata ccgattccat
    10921 tatcagaaac aagtaaataa agattgcgat cggtcgccga gataagaaaa tcaatttcac
    10981 catcctcctc agtatacttg acggcattat cgaaaagaat ggtcatcagt tgtttcagaa
    11041 gaagctgatc tgtgacaatt gttcgatgga tacgattttc aaaacggaag acacgattat
    11101 tttccgaagc aatcatctca tagtttgtga aagttgtatt aaaaaagcta gttggaactt
    11161 ctgcaagctc cggcttaatc ccatcatctc tccgagctaa gttcagcaag cttgtcgtta
    11221 aaaaacgcat atttcggact tcttccaaac tcgatgcaat gctttcgctc acatccataa
    11281 tggtagcttc tggcttacga aaaagggtct ctaagcgatt ttgcaaaact gcgagtggag
    11341 ttcgtaactc atgactggca ttttccacaa aagactgttg cttctgcata ctctcaagca
    11401 agggcctaac actgacccta gctagataga gactggcaag taaagacaaa atccagaaac
    11461 tagccatcac gaccacaatc aattgctcat gcttttgact agcctgctcc aactgactgg
    11521 tattaatcaa gacagcagca tacttgatat tggttgaaac cgaactaata ttggtctcca
    11581 tcaaaatcac acgatagatt tcttcctgcc catagctatt aaaaacctga atctggtaga
    11641 tatgtcctag ttctttcttt tctaacttaa tcttatccaa gcccaaaaat cgatttccag
    11701 aaagaagttg agtaaagtct ttatcaaaga gaatgacttc cgtattggaa ctgacattag
    11761 gttttatttc tactttacta gcgtccgccc tagcattttc taaatcttta atctcttctg
    11821 ttgccctatt tatagccagc tgaataactg cttgaggatt ttcactcagt ccatgaagct
    11881 tatcgtccac cgaagtataa agactcgaat gcatgacttg taaaataatc agagtcattg
    11941 tagaaaaaat cagggtgaag acaccgaagt tgcggataaa ataactaaag tcatccgcat
    12001 accatgtttt tttaagttta ctgaacatct tttaaaagat acccaacact acgcaaggtt
    12061 tgcaaattct ctgcaaaagt ggttcccttt aatttcttac ggacttttga aacatagact
    12121 tcgacaaccg aaatcgttgt atcactatca aatccccata gacggtcaaa aatctgcgtc
    12181 ttaggcaaaa tcacattttg attttgaagg aaataaacta gtaaatcgaa ctctttcccc
    12241 agcaattcga caggagtatc ttcaacttta acggtattgg ttgataaatt aaccacgata
    12301 ttcccataag tcaaggtgtt ttcattaaac ttccctgaac gtttgagaag ggcctgaatc
    12361 cgcattttaa gttcttctag gtagaaaggt ttggtcagat aatcatccgc tcccagttca
    12421 aatccatgtc ccttgtcatc caaactttcc ttggcagtca taatcagaac tggtgtcgta
    12481 attccctttt cacgcaattc ttttaagact tggaaaccat ttttttctgg caacatcaaa
    12541 tccagcaaaa tcaagtcata gacaccactc tcagcttcgt agagaccttc ttctccatca
    12601 aatacctgca taacatccgc aaaatcgtct aaaaagtcaa atactgaatt tgacagacct
    12661 aggtcatcct caaccaataa gatttttatc atgagaaact cctccttatt aaaactatta
    12721 taccaaattt gccttaaaaa aaactcaact ctctgcattt tacatgagat agctgagttt
    12781 tctttttatt ttaggcttat ttatgcattt ccgtattgaa gaacaactgc ttcgactgca
    12841 gctttttcac ggctaatcaa gtcaacacgt gctgcaattt ccttgattcc cataccgatg
    12901 ttacggctaa gagcaaggtc agaaagttgc ggctcaaaga actccttgta ttccgccaag
    12961 cgttgctgag tcttaaatac atgagcagga aggataacaa agctatcaaa gctcatatct
    13021 cctccaaggg ctgccttaat ccaagcccag ttttcacgcg cccaagacca agctgttttc
    13081 tgagttgctt gatgagctag gaattggtaa taccaagcag acaagtcctg tggtttgacc
    13141 acaaatttgt ccttccaaga agtaatcaag ttttggatat tatccgcatc tgtactgtat
    13201 gcaagagctg ctgccaactg gcgtttaaag acagcatctg ttgcgtgagt ataagtatca
    13261 agataaagtg ctaacaagtc tttagtctca tgatgtttca tctcattaat cagaacttgt
    13321 gaacgaatag ctgctgggag tcctgcaaga ttctccttgt gtgttgcgaa gatttggcta
    13381 gcgacttgac tagcttctgc atcatttgag cgaatcatca tcgaaacagc cagctgacga
    13441 accaattcat cctcatctga ttctccgtct ttagcttcaa aaccaagacg gtcatagtta
    13501 tgacgagcca atttagcaac cagtcctttg aaggctgttt cagcatccgt tccttcatca
    13561 ataaagcgct caagggctga aatcacttga gaaacagctg aaaccaccag ataagactct
    13621 tccttagcaa gtttatcaag aactggaagc aagtctgcat aagaaatgtg ccctgcctca
    13681 gccaacaaac gacgttcttg aacaatttgc agtttgcttg tgttatcaag tgtctctagc
    13741 tcagcaagaa cagcttctaa caagtctcct tgatagtcgg taatatagtg ggcagtattt
    13801 tcagtgttga gacgaagagc tccttcattt tcagcaagaa gagctgcgta gccagggatt
    13861 tcgatacttt cagtttcgag tgtatcaggc aagcctttcc agttgctatt gaggggcacc
    13921 acccagagac ggttcttgtc ttcgttctca ccgatgaaga attgtttttg tgaaatcttc
    13981 aagacatcat tttcaacttt aacagtaaga actgggtaac caggctgttc caaccaagaa
    14041 tccatgaagg ctgcgacatc acgtcctgac gcttgaccaa gggcatccca aaggtcacta
    14101 ccaatggtgt tgctgtattg gtgtttttca aagtaggcgt gcaaaccttt agcaaaatca
    14161 gcatctccta gccaacggcg aagcatgtgc atgagacggc ttcctttggc atagacgata
    14221 gcgccgtcaa agagtgtatt gatttcatct ggatgtttaa cttcgacgtg gacagactga
    14281 acgccatcag tagcgtcacg ttcaagagca agaggtactc cacctgtttg gaaatcttca
    14341 aagatattcc agcttggttc gatggtatcc acacagacgt attccatcat attagcaaaa
    14401 ctttcattga gccaaaggtc atcccaccat ttcatagtca cgaggttccc aaaccattgg
    14461 tgagccaatt catgggccac aacaagggca acttgttgac ggctagcaaa tgtagagttc
    14521 tcatcgacaa ccaagtaaac ttcacggtag gtcacaagac cccagttttc catagcacca
    14581 gctgagaagt caggaagggc gatgtggaga gattgaggaa ttgggtactt aactccatag
    14641 taatcttcgt aaaactcgat agagcgaaca gcgatatcca gtgagaaatc aagatttgaa
    14701 agtggatgtg ctttggttga gtagacacct accagggtac catttttagt tttagcggtc
    14761 accccttgca aatcaccagc aacaaaggcc aacaagtaag aagacatgcg aggtgttgtc
    14821 tcaaacttcc agatacctgt ttccttacgg ttttcaacat cgatttctgg catgtttgac
    14881 aaggccaatt caccttctgc ttggtcaaag cgaagagaga gatcaaaagt tgctttggct
    14941 tcaggctcat ccacacatgg gaaagcttcg cgcgcaaaat ggctctcgaa ctgagtagac
    15001 aagacctcct tcttgactcc atcaactgta taataagaag ggtaaatccc tgtcatgttg
    15061 tctgtaattt taccagaaaa ggcaagaacc aattcaactt gaccagcctc agccaattcg
    15121 atatgaaggg cttcattgtc atggtcaact gtaaatggac gagcttgacc tgcaacttct
    15181 acagaggtga tttccaagtc tttttggtgg agggagatgc ggtcactctg tgcttgacca
    15241 gtgatggtca ctttcccaga aaaagtcttg gtctcacgac tcaaatctaa aaataaatca
    15301 taatgttcag gaacaaattg cttaataaaa tgttcaactg cttgcatagt tttctcctat
    15361 tctaagttta agagctgctg ctcttcttca aacaaactta ttatatcata ttttgcttga
    15421 gaaaaaagga ttggacagcg atttccaaaa ctttcttcct tctaatagtc tacagtgggt
    15481 agaccttgcg aaattcttct aaaacaacct tgctttcagg tgtaaaagtc agccctactt
    15541 cacccaaaca gtctgtgaaa aagtcctcat gagcctggcg ccaataggca agtgatttgt
    15601 ctccctcacc ttccttaaag gcatgttgcg cagagacttg attgaagagt tcaacagaaa
    15661 cctttgtaat ttcgacaatg cagacagact gattttgact atctaaaatg acatcgaagg
    15721 tccctacttg gggaagtggt tcgtcttcta gtacatagag gtcataggct gatgctgttg
    15781 ctgtcttttc tcctttaaac accaaatccg ctaaaaggtc tggttcaact ccaaaagccc
    15841 aagcatcgat ttcatctccg atcaaaggat tgatttgctt gtatttattc cacatttctt
    15901 gcggtatcat gggtactcct ttgtaatttt ttactttctt cttttatgtg tttaagatga
    15961 tctggatggt caatctctaa atcaaaaatc tctggaatag aactgtagtg gataatgcac
    16021 ttgataccca actgattcat tttttgtatg aaagaagtat tcagatagcc tgctacagca
    16081 aaattaatct tgttctttct tgctttatcc tgcatatctc ttagcatatc taacattatt
    16141 ggactttcca tatcatgcca ttgactgttt ctcatagtcg caaaaacaaa ggaagtcaaa
    16201 tcattcattc caactacaat ctttgaaatg cccgtttcca gtatactaga taagtcaaaa
    16261 tacgctgacg gtaattcaat catcgttccg actttcccag taaagccata ctgacgcaat
    16321 actgtaatag cttgttttaa ttggtcggca tcattgacag aaggaaagat aagagataga
    16381 ttggggttgg tttgataaac ttctgtaacg acatgtgctt cagcctgaaa ttcatccaaa
    16441 cacgccagta aacgtctggt tcctctatag ccaaacaagg gatgcccttc gtcaaaaaac
    16501 tctctagtcc ccactaaaca attggcttct gtattcgtta attcagaaaa acgataccaa
    16561 acttcttcat ctgagtacag ggagcagata gtctctagat aatcttttac aaattgctga
    16621 caacttgcta atatgatgtt ttgattaagc tctctcaaca aatattcccc acgaatcatg
    16681 ccgacgtggt gaaatagttg aggataaatt ttttcaagaa ttttttcgcc actaagggca
    16741 agttgatttc tcatagccat tcacctcgtc ttaaaagata caatatcata tataattata
    16801 tcaaaatttc ctctagttca aaagcttcat aaggttcaga taactcatat cctaaaaatt
    16861 ctgctacttt tttggaagcc tcattatgag catcccaaag tggaaacata tctctattta
    16921 aactctctag aatcattgca gcaccaagct ttttagcaaa tccatttcct tgttcgtttg
    16981 gtctagttgc aacttccact tcaactgctt tacggtaaac taaccctgag gaaatcccag
    17041 caatcagttc attattttta agaatcacaa agccaaatcc accttttaaa acaaaatcct
    17101 gataggactc aaaatccccc tgtaaatcct gtgaccattc ttccgtagaa aagcagttat
    17161 aaatatgatt atcaataggc acaatattgt aaccttcctc taaatgagta actagattat
    17221 ttaaaaattc aacttgaaaa tttgccttat ctttaaaaga atagcgagta aaagaattta
    17281 gccccacttt tcgatgcaga aaatcttgcc aattagtatt ttctgaaata ataatcttat
    17341 aatctaaacc atattttcta acaaaatctt cccaaaatct ggaatccaac tcccctgcta
    17401 ggtatagaaa attcccaaca tcataaaagc ttgttgtcct atcattgttt cggtagacag
    17461 ttccaatttg agatttaaga ccatagagaa ccatattttt cttccatgtt ttaaatagtt
    17521 ccattatccc tctacttcac cttccaattc atgtaagaag tcttgtccag ttctggcaat
    17581 cctaataatt cagacttaac cttcaagact aatggcgatg cattttcttc tgtaatctct
    17641 tgaatatcca tccaaatata tccaagtgaa tcattcgcac catcagacac agcttccgaa
    17701 atcgtaactt gaggtgcact ctcattcatt tcaacatcat acaaggctat gacatggtga
    17761 accataaaat tttttaactc ttccctgacg aaaacatcgt agattcgagg attagagtag
    17821 cttctaacag taaatcccgt ctcttccata acttctctag tcagcgtttc cgtcagtcct
    17881 tcaccaagtt gctgactgcc tccaggtaga tcataccgat gttgataagg gcctctcgtt
    17941 ttttcaatgc aaagtaactt tccattttca aagcaaacac agtagacccc aaagtgattt
    18001 ttgatttcca tccaactcct cctacttcaa agaccagcca ccatctattg tcagaatttg
    18061 tccctgcatg gcagatgcct ttccactggc caaaaagaga ctgacttctg ccacttcctc
    18121 tggttcaatc cagcgcttga ttggtgtttc actagccacc cagtcagcta agccacctgt
    18181 ctcaaaatcc gcagcggtca tagctgtctt gactgctcct ggagcgatac caaagacctg
    18241 aatcccagct tcagcatagt ctagagccaa ctgcttggtg aagccagcca aggcatgctt
    18301 ggatgaagta taggcgtgac cacctccacc tgctaggcta gaagcaatgg aacacatatt
    18361 gatgatgatt ccctttttat tttccagcat ttgtgtcaaa taataccgag tcaactctac
    18421 tggaataatg tagttgattt caaaaatctc ttgaatgtcc tgcgccgttt gttccaacag
    18481 tggtttgtaa tcatccaaaa ctccggcagt attacacaaa acatccacct gagggcacca
    18541 gtcaaaaata ggttccaagt ccaaggtcaa atctctctgt aaaaagtgaa aatcaccctc
    18601 taagagtggc ttttcacctt ggtcaactcc ataaacttga tagcccttct ctaaaaagag
    18661 gcgagcttga gccaatccga tccctgaact cactcctgta atgagtacac gtttagtcat
    18721 gcacttctac ccaatccgtt gccaaaacat cacaaactgt cgggctccac atggaaaaac
    18781 cttctccttc tccagaaacg ttgattagga aataaggtgt catttcaagt gcaagcccat
    18841 tttgctcgat ggtatcaaag agttggacat agttttccgc acctccccaa ccagttcgta
    18901 catattttct cttagccttt aacccaggca ggatctcttc aaatgtcatg tttttctcct
    18961 ttaattctac attcttcatt taattatagc aaaaaaccac tttatacggc tttttgactg
    19021 tgagttattc aaacctgcta ctacttacgg caaattattc cctgcagcaa gataaatttc
    19081 ataccattct tttcttgtta agctaaagtt tgccgctcgg ctaacttctc tcaagtgctt
    19141 aggatttgtt gtacctacga ctgcctgcat ttttgctgga taacgcaata tccaagaaat
    19201 ggcaatagtt gaagaggtta ctccatattt aatagctaaa cgatcaagta cttgatttaa
    19261 agcttgaaat ttctcatttc caacaaagtt ccctttaaaa tacccgaatt gcaagacaga
    19321 ccatgcttga atgaccacat cgtgtaattt gcaatattca aaaatgctgc catctcgcat
    19381 agctgcttga ctatcttcca tattaacatg aaaagctgat tcaaatcctg gagtaaaagc
    19441 cgcactcaat tgtagctgat taacagctaa cggctgcttg acatcttttt taagcaactc
    19501 catcatcata ggattttgat tagaaactcc aaaatctcga actttacctt gtttataaag
    19561 gagatcaaag gcttctgcta cttggtcaga ttccatcaaa gcatctggtc gatgaaggag
    19621 caagctatct agatgatcaa tcttcaatct ttgcaaaata ccgtctactg attttataat
    19681 atagtcctta gaaaaatcaa aataggtaaa ttcttcaatg cgaatgccac atttggactg
    19741 aatccacatc ttttctctta aatctggacg attttttagg acaagaccta acagttcttc
    19801 acaacgacca cgaccataaa tatcagccaa gtcgaaggca ttgattccaa cagaaagtgc
    19861 tgtttctaca agctcttcaa cttcttttac agacttatct tttattctca tcattccgag
    19921 aacaatttct gataattctt tgtcatcttg accaagagtt atgtatctca tcaaattttt
    19981 ctcctttaat ttctaacatt cttcccttca ttataacaaa aaaccgcttt gcaacggctt
    20041 tttgactata cttcactcca ttttatcttc ttaaacccac ggaacaagac aaagattcca
    20101 ataaagagga cagctaaagg aataactttt gtaaggaaaa catttgaaat tcccatctac
    20161 tcataataac ggagcagaga acccaccaca agatgggcaa taatcatact gacaaatgga
    20221 cgaaagaccg cttctttcca attccaaata ccgataacta gcgaaatcgt aaagacagac
    20281 aaactatccc agggagccgg aatataaaag gctccttctt gtatgaagct tgccattcct
    20341 acatatccta aaacaactag aagaactata gtcccaacaa caatgtaagt gccaattttc
    20401 attttaggag aatcttggac taaacttctt cgtaaaattg tggccacaag tccaaatcca
    20461 atcagaaaaa taagaagttg ccctaaaaat gtgagcaaat tgactgttaa gagaggacct
    20521 ttagaaaaat cacttagtag ttgataataa cgtaataccg ccaggacaag aattggcgtc
    20581 aaaagggact ctttgataga actgcgaggt gctcccttga gaatctcttt cattattttt
    20641 ttaggattct tacctagata atcctctgca ctcatgccat ctcgttctgc ttctgagaaa
    20701 tctagcatca tcaaatagat ctgctctctg agatagtctt catcatagag aaatccagca
    20761 agattaaaac tttcccacaa ctcctcaaaa tacttttgat tctcctcaga aaactcatgt
    20821 agcaaagcgc ttgtttcttc gtaatacttc attttcttca tggtttaacc cccattctta
    20881 atcccttcta ctttttgact caaatcgtcc cattgttgcc aaaagactga gacacgctct
    20941 tctccttctt tcattaatga aaaatacttc cgatctggac catctggcga cgggcgcatg
    21001 tcgcctctta tccattgatt tttttctaac ttttgcaaca aaggataaat agttcctgga
    21061 acgatagtat caaatccagc ctctcgcaaa gtctgaacca actcataacc ataccgctct
    21121 ttttgaccaa tcatatccaa gacacaacct tcaagaacac cttttaatag ctgagtttct
    21181 ttcatcactt ctcccttcta atctattttg taatacctac tagtaacttc acctatagta
    21241 tatcacttct acactagttt gtaaagcata atagttaata ctcttcgaaa atctcttcaa
    21301 accacgtcag cgtcgcccta ccgtatgtat ggtt
//
LOCUS       DAVKMU010000034        19361 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKMU010000034 DAVKMU010000000
VERSION     DAVKMU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.034"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(101..250)
                     /locus_tag="VW343_001456"
                     /pseudo
     CDS             complement(101..250)
                     /locus_tag="VW343_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(404..2035)
                     /gene="rlmD"
                     /locus_tag="VW343_001457"
     CDS             complement(404..2035)
                     /gene="rlmD"
                     /locus_tag="VW343_001457"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9755994.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKIFMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2109..3083)
                     /locus_tag="VW343_001458"
     CDS             complement(2109..3083)
                     /locus_tag="VW343_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9755995.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3157..3771)
                     /gene="pmp23"
                     /locus_tag="VW343_001459"
     CDS             complement(3157..3771)
                     /gene="pmp23"
                     /locus_tag="VW343_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9755996.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQKGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3771..4745)
                     /locus_tag="VW343_001460"
     CDS             complement(3771..4745)
                     /locus_tag="VW343_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9755997.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4753..6009)
                     /locus_tag="VW343_001461"
     CDS             complement(4753..6009)
                     /locus_tag="VW343_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9755998.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6071..6502)
                     /locus_tag="VW343_001462"
     CDS             complement(6071..6502)
                     /locus_tag="VW343_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9755999.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSLEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGEGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6504..7106)
                     /locus_tag="VW343_001463"
     CDS             complement(6504..7106)
                     /locus_tag="VW343_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9756000.1"
                     /translation="MIDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7090..7929)
                     /gene="prmC"
                     /locus_tag="VW343_001464"
     CDS             complement(7090..7929)
                     /gene="prmC"
                     /locus_tag="VW343_001464"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9756001.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     RQD"
     gene            complement(7929..9008)
                     /gene="prfA"
                     /locus_tag="VW343_001465"
     CDS             complement(7929..9008)
                     /gene="prfA"
                     /locus_tag="VW343_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9756002.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLKELNK"
     gene            complement(9018..9497)
                     /locus_tag="VW343_001466"
     CDS             complement(9018..9497)
                     /locus_tag="VW343_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9756003.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIVISSGNYLLFCFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(9487..10086)
                     /locus_tag="VW343_001467"
     CDS             complement(9487..10086)
                     /locus_tag="VW343_001467"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9756004.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPIMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10210..10392
                     /locus_tag="VW343_001468"
     CDS             10210..10392
                     /locus_tag="VW343_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9756005.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10529..11902
                     /gene="mnmE"
                     /locus_tag="VW343_001469"
     CDS             10529..11902
                     /gene="mnmE"
                     /locus_tag="VW343_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9756006.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11949..12884)
                     /gene="dapA"
                     /locus_tag="VW343_001470"
     CDS             complement(11949..12884)
                     /gene="dapA"
                     /locus_tag="VW343_001470"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9756007.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12940..14016)
                     /locus_tag="VW343_001471"
     CDS             complement(12940..14016)
                     /locus_tag="VW343_001471"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9756008.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14358..14430)
                     /locus_tag="VW343_001472"
     tRNA            complement(14358..14430)
                     /locus_tag="VW343_001472"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14395..14397),aa:Thr,seq:ggt)
     gene            complement(14530..15039)
                     /locus_tag="VW343_001473"
     CDS             complement(14530..15039)
                     /locus_tag="VW343_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9756009.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIQKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15037..15133)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15162..15593
                     /locus_tag="VW343_001474"
     CDS             15162..15593
                     /locus_tag="VW343_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9756010.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            15675..16052
                     /gene="mscL"
                     /locus_tag="VW343_001475"
     CDS             15675..16052
                     /gene="mscL"
                     /locus_tag="VW343_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9756011.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16094..17188)
                     /gene="hemH"
                     /locus_tag="VW343_001476"
     CDS             complement(16094..17188)
                     /gene="hemH"
                     /locus_tag="VW343_001476"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9756012.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17266..18141)
                     /gene="srtG2"
                     /locus_tag="VW343_001477"
     CDS             complement(17266..18141)
                     /gene="srtG2"
                     /locus_tag="VW343_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
                     /protein_id="HEU9756013.1"
                     /translation="MTVQKRARFKNVFLVFFCVFVALFSWQRVVEASDYDHYNPIEKD
                     ASSTGFETLQHLNKDVCGWISLDGTKVDYPLLQSQDNVKYLDRNAFGDYTIMGSIFLD
                     YRFNPNFTDFNTIIYGHSMASGAMFGEIKKFADKEFFDQHRYGSIYYNGRERGLEIFG
                     ILEVDAYDTEIYRTLSSKDEEHQAYYQYLLSKAKYKRDVSLTSTDKIVLLSTCFLNIT
                     NGRHILLAKITDAPVKVAQDESGEAVGTRYFDHGIPSHWIYILAFLLLIIVLLLLVLI
                     ILIIRRDKKTKEQKK"
     gene            complement(18219..19217)
                     /gene="srtG1"
                     /locus_tag="VW343_001478"
     CDS             complement(18219..19217)
                     /gene="srtG1"
                     /locus_tag="VW343_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HEU9756014.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19235..>19361)
                     /locus_tag="VW343_001479"
     CDS             complement(19235..>19361)
                     /locus_tag="VW343_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate-transport permease PitB"
                     /protein_id="HEU9756015.1"
                     /translation="VTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLSKKRREA"
BASE COUNT         5654 a         4111 c         3651 g         5945 t
ORIGIN      
        1 accaactatt tattttttga gttttaaact ttggtagaag tcttgaactc cttatattta
       61 cataactatt tgcctctata actgttatga cacgaaacca acaagcaagt tcaagcacct
      121 ccatatcttt attcaaatga ggactaagat attcacaaac tttatcataa acctctttat
      181 cttttttcat aaaaggggca tacaacttag caaatttatc ctaaaatttt ttgttgcctt
      241 tatcaatcat ttcaacgctc ctttaaatat aaaaatgatc ctatcaatct ttatattgag
      301 cctattttct aattgatacc ttgcaagaaa ggaataatag ctgttcctat accaagtagg
      361 aatgtaataa atgcagaaag tcatatactg tcattctttt ctattaaatc attttgaagt
      421 gtctcaaagc atccatgatg gcttcttctt tttcaggtgt acactgtgga ataatttgtt
      481 tatccttttt agacttgttg taatgttctc gtaattctat tccacatttc ttttttatct
      541 gtgcaatata taatgtcgaa acttttaatt caaatttatt ccaaacatat tctttgattt
      601 gagcatatgt tgctttgctc tccgcacttg tcaaatccat ctcatccagc tcaatttcaa
      661 cacttatgtg cttatcgaca tcaagtttgg acaaaagtgc taccgtctcg acgtgatgcg
      721 tttgaggaaa tagatccacc ggctggactt tcttcaattc atatcccaat tcttggtaga
      781 gtttgatatc acgcgccatg gttgcgacat tgcaggagat ataggcgatg cgatcggctc
      841 ctgtttgggc gcttgctttg ataaagcttt ctgtcaagcc cttgcgtgga ggatcaacca
      901 agataacggt tggttgaatc ccatctttaa gccaattctt catagcattt tcagctgtgt
      961 cacagacata gtgggcgttt gaaatattgt tcagctgagc atttttttta ctattctcaa
     1021 ccgcttctgg aatcagttca acaccgtaga cttctttgac atgcttggcg actgataaac
     1081 caatggttcc aataccagaa taagcatcaa taaccacgtc atcttttttt aactctgcaa
     1141 agtcaatggc tgtttgatag agtttctccg ccatttcagt attgacttgg taaaaggctg
     1201 ggccagcgat ttggaagtca tttcccaaca tctggtccgt aatatagtct tgaccataaa
     1261 gagtgcgcca ctccttacca aaaatcgcat tggtattctg gtcgttgata ttttgcatga
     1321 cagacacaat ctctgggaac tgcttgataa cttgttcaat caattggtca acacgaaaaa
     1381 cttttggacg agttgtcacc aaaacgacca tgatttgtcc tgaatagtga ccacgacgca
     1441 ccacaagatt ccgaatcaat ccagactgtt ccttttcgtc ataaggtttt aaatcaaaac
     1501 gacggagcag gtctcgaaga gctactacga cttggtcaat gacaggatcc tggataaaga
     1561 aatcttcaag gggcatgagg ttatgcgaat tcttacggaa aaatcctgtt tccaagacac
     1621 cattcactcg acgaacgggc acctgcgcct tattgcgata cttgactgga tgttccatac
     1681 caagcgtttc agcaacttct acatctgcaa ttccagcaat cttgtagaga ctgtccttga
     1741 cttgcttggt tttaaacttg agctgttctg gataagaaag gtgtcctaaa tccgcgattc
     1801 ctgaacgcag gtaagccaaa tctagatctt gattacggtg tggtgactgg acaaggtatt
     1861 tttcaacttt tccaaagcca atctttttat tgaccttgag gacacgcatg aaaatttttt
     1921 cactcggtaa agcattctct acaaaaaaga ccaaaccatc taccttggca actcctgccc
     1981 cttcatgggt caaatcaaca atttcaactt ctacaatatc atttttcttt aacattcttt
     2041 tctctttcta ttggccgaca aaaaagacgg caacgttacg gttaccatcc attataccat
     2101 gaaatttctt aagaaccaaa actcttgaag aagttgacaa gggcttgcca gagggagctt
     2161 aagaagttac caccgtcctc tagcgcctta tccgcatcaa agttaaggtt gatattttta
     2221 aaactgtcgc cagcttgtga tacgatgctt tgtttaaggt catttagggt tttagtgaaa
     2281 tctgcattgc tgaggatatc actctttgag agattcaagg caaaattgat gatgatattg
     2341 atctggtttc ctgttatgac ctgatcaagt ttgtaatttt ttaaggtatc ttcaacaatc
     2401 ttgcggatat cttcttctgt cagatttccc ttgctttctt tagctttggc gagtcctgac
     2461 ttgatatcag ctagggcaac gtttaattta ttagcatcat agcctgattt gtccttgttt
     2521 tcagcattga tatctgacaa agcctttagc tcttcttgag ccaaatcttt attggcttgc
     2581 ggcaccttgg ctccattagc ttctagcgaa tagtagatcc cagccaaagc actctcacct
     2641 gtaactggaa tgggtgctgc tacagtgatt ttggcgtgtt ccacacccaa agttacggct
     2701 gcatttcggt acatatcctg agttacctta gtgatgtttt caggtgtttc aatcttgacc
     2761 tcaagtggcg atttgtcacc tagcttttga atcttggctg atgaatacaa ctgtaagcta
     2821 gagtcattgg ccacattcat gattttagaa taaacatcag gtgtcatggt cttgagttct
     2881 ttggtatctg ttgaggcatt gtagcccagt tttttaagag tttgattttt ttggtcttca
     2941 gatagggaag aacctaggac atattcaggt tggacatagg tttcatcgat aactttttga
     3001 acatctgttg ctgcatggac gctattcata gctgttactg cccacaagat cgcagcgcta
     3061 gtcagaaaga gtttctttct catagggaat ttcctccttt acttctttag agtaatatat
     3121 ctatcttaaa gaaaacttat aacaaaaaca cctggtctag ccagatgttg aaaagagagt
     3181 gaaacatttg atgatgtaaa ggttaagtcg tacctgtcta gaataataat agtttcctcc
     3241 atttacatag agttcagcac cgtgaaaaat ggaaatgggg tgaatataac tataagtctt
     3301 tccagtcgta ttaccaagca agggggcaac agtctcacga gagtactgtt tggctagagc
     3361 caaggtattt tccttgcctt tttgggcgat aaaatcgata taggcaggtc caaaattata
     3421 ggcttgaaca gctgtccaga tatctacacc cttcttctgc gccagataga gattgcctgt
     3481 cagagtttga atgccttgcc gaatgctaga ggcattatca ttgatggtgt tggtggaacc
     3541 acttgcagac tcactagact gcataacatc gccttctttt ccttttgttt cagtataaat
     3601 catagcaagc acaagctctt cgtttgctgg ggtgtctttt tcactcaata tttctcgcac
     3661 catgggttga taggtcatga cttgtttgac atcttgatga acgcggtaag ctttatagcc
     3721 agcaaaaagg aagactgcta gtacaagcac tcttcgaatt cgtttaaaca ttatttactt
     3781 tggatatcct cgatattttt gattaagata gagtaggttc cattttcgtt ttggataaac
     3841 tcaacagact cggcgtcttg atagacgtta ttgggaacga tgagctcaat tccatttgat
     3901 aaggagagtt tttggttttc aaatttcttt aattggcgac tggcatcaat ttcatcaaat
     3961 tgaacaggtt ctggtacggc ttctttgact tggtcaataa agctcaaacg agccgtcaga
     4021 ttgttgtcaa aaaggtcatt agccaatttc tcaggtgaca attcattgct ttcttctagg
     4081 ttgttgaaaa tagctgattt gaccttggat tgaaattgaa aatcatctgt gttaaaagat
     4141 tcagcaattc tctgggctgt tttttccagt tccttgatag attttttagg agaaatctta
     4201 ggagcgacag caagaagatt atctgaaaaa tagttcaaaa aagtcccgtt gtacttgatt
     4261 cgtttttcaa tcaggtgata cttgcgactc tgaagattga ccaccaaggc ctcgtcagca
     4321 cccgttccaa atccaggcag gttattctga gtcagcttga ttggattatc aacttctcct
     4381 ccgaggtggg tcaaggtctc ccgcagggca attcgcaaga aagcgaaatg ttctacacct
     4441 tctttagaaa attgaacaaa aatcaagtca ttggtcttga gattttcaga aatgctaaac
     4501 tcctctttcc agagattagc cagcgttact gatgtctcca acaaatcgtc tgtaatatga
     4561 ttgaagaagg gattttcttc ttcgaaaatc ccagtcttgg cttcatctga atacacatgt
     4621 tcaatttttt tacgtaggta ttcttcgatt tttggagtaa tattgagaaa cttatctgct
     4681 aagaacagct cggtatcatc cggactgaac tggtgaataa tggctttctt aatataaatg
     4741 tccataaaag ttttattcct cgtataatgg gaaggcatct gtcaattctt tgactgcact
     4801 tctcacttct tctaatacag cctcattttc tgaattctta agggttttaa tgatgagttc
     4861 agccactttg cgactttctt cttcaccaaa tccacgtgca gtaatggctg ctgctccgat
     4921 acgaatccca cttgtcttga atggtgacaa gctttcgtaa gggattgagt ttttatttaa
     4981 ggtaatattg acttcatcca acaagttttg agcaactttg ccgttttcta caactttagt
     5041 cacatcaaca aggaagagat ggttttcagt tccacctgaa ataatacgga aatcagggtc
     5101 ttgcaagaag acatctgcca tagccttgct gttcttaatt acattggcag catattcctt
     5161 gaaggctgga tccaaaactt ctttgaagga aactgcctta gccgccacaa catgctctaa
     5221 aggaccgccc tgaatacctg ggaaaatagc tgaattgatt tttttagcaa gttcttcgtc
     5281 attggtcaaa atcaaaccac cacgaggacc acgaagggtt ttgtgggtcg ttgttgttgt
     5341 gatatgagcg tatggaactg ggcttggatg aaggccagcc gcaaccaaac cagcgatatg
     5401 ggccatgtcc accatgagct tcgcaccgac agcatctgcg atttcacgga attttgaaaa
     5461 atcgataatt tgagaatatg ctgaagcacc agctacaatc agttttggtt ttacttcttg
     5521 ggcttgtttc aagatagcat caaagtctaa gagttccgtt tcaggatcaa cactataaga
     5581 aacaaagttg taggtttgac cagagaagct aacaggagcc ccatgagtca aatgaccacc
     5641 tgatgccaaa tccattccca taaccgtatc acctggctca atcaaggaca tgtaagccgc
     5701 acagttagct tggcttcctg aatgtggttg gacattggca aatttagcac cgaaaatttc
     5761 ttttgcgcgt tcaatagcaa gagtctctac aacgtctact acatcagttc caccataata
     5821 acggcgtcct gggtaaccct cggcatattt atttgtcaag atagaccctt gagctgccat
     5881 aacagccttg gaaactacgt tttccgaagc aattaactcg atattatttt gttggcgttc
     5941 ttcttctttg gcaatagcat tccagagatc agcatcatat gctttaaaat catctttgtc
     6001 aaaaatcata ggtcttctcc tttattgtgt gactagtcca ttagtttgat tttacaataa
     6061 gaaaatcaaa ctaaaagatg cgaataaacc gtttctgcat tttatcacaa gtatagccaa
     6121 ctttttcata aaatgcatga gcacccagac gatgattggc agaatttaag cggataaacc
     6181 cataaccaca tcttttggct tcttgttcca acccttgtag taaactttta ccgatacctt
     6241 caccttgcgc ttgaggtgaa actgctaaag ctaagatatt aaatcctgct ttggaataga
     6301 gtgattcgta aacttcagcg tggacatatc caagtaagac atgattagct gcatcctcat
     6361 agccaagtag gaaatgatgg gaatcctgag acagtctagc tagttggcta gccgtttcct
     6421 ctagactaaa agtataaccc aaagcctctt ggttgatgtc acatatcgct ttcacatctg
     6481 tttcttgcaa atctcttagc atttcaagcc tcctcaaaag aaatctctgg caaccgagca
     6541 agaatatctt ctcgtttaat tgcgccttgg cgtaagattt tcaccttgtc tccagacaaa
     6601 tccacaatag ttgaatcctg tccagttaga aaagcatcgt cttccagacc cagaacctct
     6661 tggtcaaaat ccttcagaat ttgttcaaag gttacaccac ttgcctgacc tgagatattg
     6721 gcagacggcc caatcaaggg acctgtctct cgaattaaat ccagtgtgat agggtgactg
     6781 ggcatccgaa atccaatagt tgcaaggtca gaatttaccc aatagggaac tcggtcattg
     6841 gcttcgagaa taatggtcaa gggacctggc aaaaaggtct ctacaagttt ttgtagataa
     6901 gttggctgat tcttagaaaa gtgcaagata tcctcgaaag aggcgatatt gagattgagt
     6961 gccttatctc taggacgacg tttgagttgg taaacatggt caactgcttt ttcatctaag
     7021 gccttggcaa aaagaccata aacagtctct gtaggtagaa cgacagctcc accattttcc
     7081 aactcttgtc taatcctgtc tatcatcaac cacgaccatc ctattttgac caaattggtc
     7141 cttgagtgtt cgaactcttt tttcaggaag atgtttccta aaaagttcgg gaacactttg
     7201 accttgcttg tatccaattt caaggtaaat cttaccacca tctttgagat agtcttttgc
     7261 atcttccgca attctacagt aaatagctag gccatcctcg tctgcaaaga gagctagatg
     7321 aggctccgaa tgcaaaacat tcaagcctac ctctgactca tcttcacgag agatataggg
     7381 tggattggat acaattatat catatttttc agaaatttct gcaaaacaat cagatttttt
     7441 aaaaaatata ttaagatttt gattgctagc attctctgat gcaagctcta aggcctcttg
     7501 agaaacatct gctgctgtca ctgaccaatc tggtctattt tttgctagag cgagagcaat
     7561 agctccacta cctgttccaa tatccagaac tgaaagattc gtctcaagat tttcagttag
     7621 aataagctcc actaactcct ctgtttctgg acgaggaatc aaaacccgtt catccacctt
     7681 taaatgcatt ccataaaaat ctgcctgtcc aatgatgtac tgagctggct tgtgagctgc
     7741 tagttgctgg taaatatctt ctacaaattg tttttcttcc tctgttgtca cctcctgctg
     7801 gagggcaaaa ataaagtctg taaaagatag atttttcaga ctacgataga caaaagagag
     7861 gctttccgct tcctctcctt gtcttatcaa ctcttcttca aaatttgaaa ataattgagc
     7921 taatttcatt atttgtttaa ttcttttagt ttttgtgttt ggtcataaag caccaaggca
     7981 tccacaactt cgtccaattt accagacaaa atcgtatcta gtttttggag ggtcaagccg
     8041 atacggtggt ctgtgacacg gttttgtggg aagttataag ttcggatccg ttctgaacgg
     8101 tcaccagtac cgattgtcga cttacgctca gcgtcttgtt catcctgagc aatctgagca
     8161 aagtggtcag cgacgcgtgc acggataatc ttcatagcct tctcgcggtt cttctgctgg
     8221 gtacgttctt cctgcatctc aaccttgata ttggttggca agtgaacgat acgaacggca
     8281 gtcgcaacct tattgacgtt ctgtccacca gcaccagagg cgtgatagat gtcgacacga
     8341 aggtcttttg gatcaatgtc gtattcaacc tcttcaactt ctggcataac aagaactgtc
     8401 gctgtcgaag tatgaacacg gccttggctt tctgtcacag gaacacgttg cacacggtgg
     8461 gcacctgatt catacttaag cttagagtat acagactgac ctgaaaccat agcaaccact
     8521 tctttaaaac caccgacacc attcatagag gcttccatga cttcaaagcg ccaaccttgg
     8581 gcttccgcat acttttggta catagttagc aaatctccag cgaaaagtgc cgcttcgtct
     8641 ccaccagctg ctccacggat ttcaaggatg atattcttgt catcgtttgg atcctttgga
     8701 aggagcaaaa ttttcagttt ttcttcatat tcttcttttt cagccttggc atctttgagt
     8761 tcttgcttgg ccatttcttc caagtccgca tctccgcctg attccttaat catctcttcg
     8821 gcatcgacga tattttgaag gacttgttta tactcacggt aggctattac ggtgtcacga
     8881 ttggaagctt cttcttttga aagctccata aaacgcttgg tgtctgaaac gacatcaggg
     8941 tcactcagca attctcctag ttcttcataa cggtcttcta cagcttgtag ttgatcatag
     9001 atgttcattt tttctcctta tttctcaatt gttaaatcat agattgctac tacttcattc
     9061 tcggatattt ccccagtttc tttaaatcca taactgagat aacaaaatct tgcctgttca
     9121 ttttctggtt cataagacaa ccaaagttta ttgcttaaac ctgctggcgc tgttcgaaca
     9181 tagtctagta ctttatccat aattggttta aaatatcctt gattttgaaa attcttatca
     9241 atcataaaac aaaatagtaa ataatttcca ctactaatta cgatcttttt atcataagct
     9301 atcatcacaa aacctataat tacatcatta tcataaactg ccaatggagc tacaaaatct
     9361 ccatttttag tgtagacgta tgcttcagct aaactaattg cgttggttgc aatgaattgt
     9421 ttttgatatt ccttgacatc caaatttaaa acatcaaaat aattttccat tgtaacatct
     9481 cttagttcaa ttgtcatagt tttgctcctt gttagaggtt atcattggcg caaaataatg
     9541 tttacggcaa accgagatat aggtttcatt accaccaatc tggatctgtt ctccatcgta
     9601 aacgggcagt ccatcctgtg ttcgcaacac catggtcgcc tttttcttgc aatactgaca
     9661 gatggtcttg atttcgtcaa tcttgtctgc taaaagcaag agatatttgg aaccttcgaa
     9721 caattcattg cgaaagtcat ttttcaaacc aaaagccatg atgggtatgt ctaactcgtc
     9781 cacaacacga gctaggtcgt aaacatggtg gcgtttaaga aactgggctt catcgaccaa
     9841 aacacagtaa ggtttttctg gtaggtctcg gatatagcca aagatatccg ttgtttcctc
     9901 aatcgcaagg gcagggcgtt tcatgccaat tcgactcgac acatagccaa caccgtcacg
     9961 cgtatccaga gccgaggtca taatgacaac accttttcct tgctcctcat agttataggc
    10021 caccttgaga atctcgattg tctttccaga gttcatggtc ccataacgat aatacaactg
    10081 tgccatgttt cttgcttcac gtccatttct aaatttttgc tacattctag tatatcataa
    10141 ttttcttaag ctttaaacgg caaaatgtgg taaaatagaa gaaatcaaaa actagtggag
    10201 gaagctatta tgccatttgt acgcatcgat ttatttgaag gacgcacgct cgagcaaaag
    10261 aaagctcttg ctaaggaagt aacggaagcg gttgttcgca acactggagc acctcaatct
    10321 gctgtccatg tcatcatcaa cgacatgcca gaaggaactt acttcccaca aggggaaatg
    10381 cgtaccaaat aagctagctt aagcagaatt gcttaggctt tttcaatctc caagtagcat
    10441 tcattgaaga aatatcctaa atttgttaca atttgaaaag aaacttggag aatttccaag
    10501 aaaatagcta ttaattaaag gaaacattat gattacacgt gaatttgata ccatcgctgc
    10561 tatctctact ccactaggtg aaggggctat tggtattgtc cgcctgagcg gaacagacag
    10621 ttttgctatt gcgcaaaaga tttttaaagg aaaagacttg aacaaggttg ccagccacac
    10681 tctcaactac ggtcacatta ttgatcctct gactggtaaa gtcatggacg aggttatggt
    10741 tggggctatg aagtctccaa agaccttcac tcgtgaggat attatcgaga ttaacaccca
    10801 cggtggtatt gcggtgacca atgagattct ccagctagct atccgtgaag gagctcggtt
    10861 ggcagaacct ggtgaattta ccaaacgtgc ctttttaaac ggtcgcgtag atttgacaca
    10921 ggccgaggcg gtgatggata tcatccgcgc caagacagac aaggccatga acatcgcggt
    10981 caaacaactt gatggttccc tttctgatct tatcaataat atccgtcaag aaatccttaa
    11041 cacacttgcc caagttgagg tcaatatcga ctatcctgag tatgacgatg ttgaggaagc
    11101 cactactgct gttgtccgag agaagacaat ggagtttgag caattactaa ccaagctcct
    11161 taggacagca cgtcgtggta aaatccttcg tgaaggaatt tcaacggcta tcattggacg
    11221 tcccaacgtt gggaaatcaa gccttctcaa caacctcttg cgtgaggaca aggctatcgt
    11281 aaccgatatc gctgggacaa cacgagatgt catcgaagag tacgtcaaca tcaatggtgt
    11341 acctctcaaa ttgattgata cagctggtat tcgtgaaacg gatgatatag tggaacaaat
    11401 cggtgttgag cgttcgaaaa aagccctcaa ggaagccaac ttggttctac tagtgctaaa
    11461 tgccagtgaa ccactgactg cgcaagacag acaacttctt gaaattagcc aagataccaa
    11521 tcgcattatt ctacttaata aaaccgacct gccagaaacg attgaaactt cgaaactacc
    11581 tgaagacgtt atccgtattt cagtccttaa aaaccaaaac atcgacaaga ttgaagagcg
    11641 aatcaacaac ctcttctttg aaaatgctgg cttgattgag caagatgcta cttacttgtc
    11701 aaacgcccgt cacatttccc tgattgaaaa agcagttgaa agcctacaag ccgttaatca
    11761 aggtcttgag ctggggatgc cagttgattt gcttcaagtt gacttgactc gtacttggga
    11821 aatcctcgga gaaatcactg gggatgctgc tccagatgaa ctcatcaccc aactctttag
    11881 ccaattctgt ttaggaaaat aagaaaaatc catgatcctt cattcggtca tggattttat
    11941 tgtctttatt agtaatctgg tcttaagacc cctgttacag ttgccttagt tgcttcgtag
    12001 tcgccatcta cgacaacctt gataatgcgt ttggcatctt cttctggtgc tggaacaaga
    12061 ggtagacgag tgggtccagc ttcaaatccc atatagttaa gaattgcctt aactggagca
    12121 ggacttggat aagagaagag agcattaacc ttaggaatga atttacgctg aattgctgcg
    12181 gctttcttca tatcgctttc tgcaatggca gtaaacatct cgtgcatttc atccccattt
    12241 gtatgagagg caacagaaat aaccccatcc gccccaaggt tcatggcatg gaaagcatct
    12301 ccatcctcac ctgtataaat caagaactct tcaggcttgt gctcaatcaa gtaagccata
    12361 ttagccaagc tagtacattc tttgacaccg ataatatttg gatggtcagc caagcgaagc
    12421 atggtttctg gagtcaattc gacaactaca cgccctggaa tgttatagat aataattggt
    12481 aggtcagaag catctgcaat agtcttaaag tgctgataca tcccttcttg agaaggtttg
    12541 ttgtagtaag gaacaatagc aagcccagct gcgaaaccac caaattccgc tacttctttg
    12601 acaaactcaa tagagtcacg cgtatcatta gtacctacac ccgcaatcaa aggaacgcgt
    12661 ccattgacaa ccttttgtac agccgcaaac aactccaact cctcatcgtg ggtcaaagtt
    12721 ggactctcag cagtcgttcc tgcgagaaga attccatccg tatgatgggc caataaatgc
    12781 tcaatcaagg ctggaatagc atcaaagtta atagaaccat cctcatgaaa gggggtaata
    12841 aaggctgtaa tgattttaca ttcttttaaa tcttgataag acatgaaaac acctctctat
    12901 ttcaaagaag gagttcatct gaactcctaa acgatatgac tattttaatt caaatttcaa
    12961 ttcggctgtt ggacgaacca atccacgttc atgaagagtt tcagcaatct gaactgagtt
    13021 ccaagcagca cctttgagaa ggttatctga aacaacccac atgtgaattc ctttttctgc
    13081 atccaagtct ttacggatac gaccaacaaa ggtatcacgc gaaccaactg cattgatagc
    13141 ttgaggatag atttgatgag ctacatcatc ttcaagaaca gcacctggga aggctgcgat
    13201 agctgctttt acttcttcga ttggagccac ttcttttgtt tcgatataaa cagactcaga
    13261 gtgagctgac aagactggaa tacgcacaca tgttgcagat actgcaatgc tatcatcttc
    13321 cataattttc ttagtttcct tggtcatctt catctcttcg tacgtgtaat cattatcagt
    13381 gaaaacatca atttgtggaa gagcgttaaa ggcgatagga taatgtttct tgtcaccacc
    13441 tgaaggcaaa atttccgcat gcaaatcaca tggtttcaca ccatcattca agacttcacg
    13501 aagttcacgt tgtgtctcaa gaattgctcc cataccagca cctgaaacgg cttgataagt
    13561 tgaaacaatg atacggtcca agccccattt ttggcgaacc ggctcaagag ccaccatcat
    13621 ttggattgtt gaacaattag ggcaggcaat gattccgttg tgggcatcaa gtgcatgagc
    13681 attgacctct ggaacaacca aaggaacatc tggattttga cggaaataag atgtattatc
    13741 cactactacc acgccagctt ttactgcgta tggtgcatac ttagctgatg tagaactacc
    13801 tgctgaaaag agagcaatat caactccttc aaaagctgtt tcagtcgttt cttcaattgt
    13861 aatatcttga tctttaaatt tcaatgactt acctgctgaa cgtgcagaag caaggtaacg
    13921 gattttatcg attggaagtg ttgattcttc caacattttt atcatctgag caccgacagc
    13981 acctgtcgcg ccgactacag caactgtata tcccataaat aacctctttc ggaattttct
    14041 aaaaatttct ataatagaag tattatacta ctttttccat gaattgcaaa gctttttcat
    14101 cattttttgg aaaataaaaa tcccagtcgc tagactaggg actaagaggc atcttcatgt
    14161 gatgagcagg ttcacacaac tcatcaaggt ccgctcctgc gttatgacct ccttatgctc
    14221 aatagtagtc cgaagactac ctatcgactc atcgcatatt ctatattact agagatactt
    14281 gattttgtca ataagaaatt ttttcgatcg agtataacaa aaaactctcg tattcaagag
    14341 tttccctata catatcatgc taattgccgg gatcgaaccg gcgacctcat ccttaccatg
    14401 gatgcgctct gccaactgag ctaaatcagc ttacttaaga agtatactat attcagaaaa
    14461 acttgtcaag tttccttaga aattttccat aggaaaaagc caggtaagaa ctacctagct
    14521 tatcttcttc tatttactta aatcaattcg acgaccaatt ttaccaataa taatcgcccc
    14581 aataatcaat gaagcaaatt caccgattcc tgttgtaaac caagtaagga agaatggtaa
    14641 ttctgctaca atatttaact ctgcagcgat agtaaacatg gaaattgaaa agaggattga
    14701 aaagaagaaa tgatcttttt gaatcaatcc gttaaagagg taatccttgc tatattttgc
    14761 aaaaagccaa acacctaaac tgaggaatac taaggttgaa ccaccaccaa caaagacatc
    14821 tagcagtccg aagctaaaga aattagcaat catacaaccg atggtaactc cgatgatgta
    14881 cttaggattg taaaaagcca taaagttcat catttctgaa atacgaaact gataagcacc
    14941 atagctgatg gcatttaggg gcggtgtgac agtcaaaacg acgtagatag cagcgacgat
    15001 tgcaatatct gcaacatcac gaatagttaa ttttttcatc ttttctcctt tggcggttgc
    15061 ccgcgtgtaa tatgcttggt gaaagaagct aagcaccaag ggttgcttta ttcaacccta
    15121 ctagtatagc acaatagcct gctttatgct atacttaaca catgaaaatt cctattcaaa
    15181 aattttttaa caatgaaatc ttagcctatc tcttttttgg ccttgcgaca actctagttt
    15241 cgattctctc acgactagtc atttatcaac taagtcatca ggaactcctg gctactgccc
    15301 tagcaaatat tatcggtatc ctatttgcct ttatcacaaa tgatacgatt gtatttaagc
    15361 aaaaaaagaa aaatagactt atccgtttag tgaaattttc tcttgctcgc ctttctactt
    15421 ttctgttaga cttgttttta acttttctct ttgtaaccca atttcctcac attatagggc
    15481 aattcgtaac tgaaaacatt gataagataa atgctattga aacaataatt gcacaaatat
    15541 tcattattat catcaattat atattgagca aggcctatat ttttagaaaa taatgctcca
    15601 tttatgagcc ttttaattgc cttctataat gatttaggat acaatgaaac acgaaacatt
    15661 gaaaggaaaa cagtatgtta aaaaatctaa aatcgttctt gcttcgagga aatgttattg
    15721 accttgctgt cggtgttgta attgcctctg cttttggtgc tatcgttact tcacttgtaa
    15781 acgacattat cactcctctt attttaaatc ccgctttgaa agctgctaaa gttgaacgta
    15841 tcgctcaact ttcttggcat ggagtcggct atggtaactt cttaagtgct attatcaatt
    15901 ttatctttgt gggtaccgcc ctcttcttta ttatcaaggg cattgaaaaa gcacagaaac
    15961 tgactggcat aaagaaagaa aaaactgccg aaaaaaaacc aaccgaattg gaagtccttc
    16021 aagaaataaa agctctcctt gagaaaaaat aatcgattaa aaggatctag cgcaaagcta
    16081 aatccttttt tctttactct ttgggtaact tgcctgcttt ttcaagcatt tttttgatca
    16141 gataaggcat cttaacattc tcccgccctt ttttctcaat cagttttttc acaaattctg
    16201 gcatttgtaa atcttcagat tcagctaaaa tgtttttcgt ctcgtctgaa ggaactaact
    16261 catcaaaggt ctcttcttct gggagaaatt ccttaaactc ttgattttca ttgactctaa
    16321 ctgtattgac caaagcatca atcaaacgag aatctgtatt tggcattggt ggacgatggt
    16381 agtttacccc aaattcctgg cataagtcat aacattctac atcattgtca aacaagacct
    16441 caatatgctc actgatgaaa ctaataggta caaaaatata atggtctgga tgttctgtct
    16501 gttctctgag atactccaaa acatctggtt taatccatgg aataccaata tcactctcac
    16561 tctgccaagt gttggtatat tgttctgaac tcaagcccag tttttcagcg actagcttgc
    16621 tattttcgaa aatttggtct atataaggat caccaaagtc caaggcaaaa atgggcacac
    16681 tatgggctga aaagatgact ttaaagctgt cttgctttac ttcttctttt aaaatcttag
    16741 caatttcatc tgcccaatag tttaagagca cttcttcctg ataccagtcc ttaatgacca
    16801 aaaattgaat ttgtttgctt tctaaaaatt tttcatagcc catgacagag tagaaagaat
    16861 aatggggctc caaaatcaag cagatacatt cttcaacacc atcagtttcc atctgcttga
    16921 tcacgtctgg aataaaggga cgagaaaatt tgttggcaaa gtagataccg tactcctccc
    16981 ctaaacgttc tttgactaaa ttgacctcct cacgtgtaat ccgctgcaaa ggcgtcccac
    17041 cgatacgaat atagttgtca tagagagtct ggatttcgtg gtcttggggt ctaactccac
    17101 gacgaatgtt tgtaaagaat tctgctactc cttcaaaact aatctcctct ggagatccaa
    17161 aggtcatcat taatatggct tttttcatgt tatcatcctt atgatattat tttctctatc
    17221 cattctatca tgaaagaaaa aaatagtaaa cgtttcctac gtctactatt tcttttgctc
    17281 ttttgttttt ttatctcgtc ttataattag gatgataagg acaagaagta agaggacgat
    17341 aataaggagt agaaaggcta gaatgtaaat ccaatgactg ggaattccgt gatcgaaata
    17401 ccgtgttcct actgcttccc cactttcatc ctgtgctact ttcaccggtg catccgttat
    17461 tttagctaaa aggatatgtc gtccattagt tatgttaagg aaacaggtgc ttaataaaac
    17521 aatcttgtcc gttgaggtca aggaaacatc tcgcttgtac ttggctttac ttagcagata
    17581 ttgatagtaa gcctggtgtt cctcatcctt ggaactcaag gttcgataaa tctccgtgtc
    17641 ataggcatcc acttctaaaa tcccaaaaat ttcaagacca cgttctcgac cattgtagta
    17701 gatagaaccg tagcgatgct ggtcgaagaa ttccttatca gcaaatttct taatctcacc
    17761 gaacatagcc cctgaagcca tagagtgtcc gtagatgatc gtattaaaat cagtaaagtt
    17821 gggattaaag cgatagtcga gaaaaattga tcccattatc gtataatcgc caaaggcatt
    17881 gcggtcaagg tatttgacat tatcctgact ttgtagaagc ggatagtcta ccttggtccc
    17941 atcaaggcta atccaaccgc aaacatcttt gttcaagtgc tgtagggttt caaaacctgt
    18001 gctcgaagca tccttttcaa taggattata gtgatcatag tcacttgctt ctactactct
    18061 ctgccaacta aaaagagcta caaaaacaca gaagaatacc agaaatacgt ttttaaatcg
    18121 cgctcttttt tgaaccgtca tcagcactcc tttctatgcg agatttctta aaaggaaagg
    18181 aggcgggaca aaaccgcctc ttttctattc acccttagtc agtaaaaggg tctgcttctt
    18241 gcattttttt tactctctct tctacgacgt aggatcaagt agaggacaag gagtaagaaa
    18301 atcaagagca agaacaatac aaacaagata aggtaccaaa tccatagtgg gattgatgag
    18361 aggaaacgtc caagagacga gtcatcaaag acactgtatg gaaatggttt tgattttttt
    18421 gtatggaaag tatttttagg gacggtgtct gtaatctttg cgactacgat atgacgacca
    18481 ttggtcacat cgagaaaaca ggtactgagt aggatgatac gatcactcgg tgagagtgag
    18541 atatcccgct tgtgcatggc gactgagagc aggtggtcta gataggcctg ctggcggtcc
    18601 tcaccctgta ttcctggatc atagatgtta aagtcatagg catcaacctc caacatctca
    18661 aagatctcga gccctttttc cacaccattg tagtatatac taccgtaacg atgctggtca
    18721 aaaaattcct gatcagcaaa cttagccaca tcaccaaaca tgaccccatt ttctacgtgg
    18781 tgcccgtaga taatggtatt aaagtcttcg aacttaggat tatttcgtgc atcgagaaag
    18841 atagcgcctg tcgctgcaaa ctcacctttg gagtccttgt tgagatactt ttcattgtcc
    18901 ttggcttgga ctaagggata gtcgatattg gtgccataga cattgatcca accgaggact
    18961 tcgggattga gttcttggag tttgctgaag cctgaaaaac tggccagctc atcctgttgc
    19021 gtcgtgacag gtcgataagc ttcgtactca cttgacgaag ctaaggagta gacttggttg
    19081 gaatcccaaa gggcgtagaa accaaagaga aagatcaaag ctgcaaaaat gagcagcaag
    19141 tgattgatca ccccatccac agcaagaacg agacgcctag acagactctt ttttggtttc
    19201 tcacgcttgg ttttcatcat ctgcccccag ccgtttaggc ttctctacgt tttttagaca
    19261 agacaacaaa tccagccaaa ccaagaccaa tcatcaaaat gaatggaagg ttatcaatca
    19321 agagaccagt aggagtaacg tcgtcgattt tgttagtaac t
//
LOCUS       DAVKMU010000035        18780 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKMU010000035 DAVKMU010000000
VERSION     DAVKMU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.035"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            200..538
                     /locus_tag="VW343_001480"
     CDS             200..538
                     /locus_tag="VW343_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU9756016.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            728..2164
                     /locus_tag="VW343_001481"
     CDS             728..2164
                     /locus_tag="VW343_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9756017.1"
                     /translation="MTTFKDRFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            2216..2454
                     /locus_tag="VW343_001482"
                     /pseudo
     CDS             2216..2454
                     /locus_tag="VW343_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
     gene            2584..2898
                     /locus_tag="VW343_001483"
     CDS             2584..2898
                     /locus_tag="VW343_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU9756018.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            3016..4995
                     /locus_tag="VW343_001484"
     CDS             3016..4995
                     /locus_tag="VW343_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9756019.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNGFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            5005..5319
                     /locus_tag="VW343_001485"
     CDS             5005..5319
                     /locus_tag="VW343_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU9756020.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5350..5856
                     /locus_tag="VW343_001486"
     CDS             5350..5856
                     /locus_tag="VW343_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756021.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5936..7282
                     /gene="celB"
                     /locus_tag="VW343_001487"
     CDS             5936..7282
                     /gene="celB"
                     /locus_tag="VW343_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU9756022.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVIIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDVFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7662..7838
                     /locus_tag="VW343_001488"
     CDS             7662..7838
                     /locus_tag="VW343_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756023.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8239..10452
                     /locus_tag="VW343_001489"
     CDS             8239..10452
                     /locus_tag="VW343_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9756024.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSKTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAIPLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNKNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQVAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GILDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPMYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLAFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTI
                     PVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELTLQGDSSIVPSNRRHRIHFKGTNVSIIELPNKNDTARFECKDNKTISLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10674..11150)
                     /locus_tag="VW343_001490"
     CDS             complement(10674..11150)
                     /locus_tag="VW343_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9756025.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11373..14452
                     /locus_tag="VW343_001491"
                     /pseudo
     CDS             11373..14452
                     /locus_tag="VW343_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
     gene            14805..15190
                     /gene="tnpA"
                     /locus_tag="VW343_001492"
                     /pseudo
     CDS             14805..15190
                     /gene="tnpA"
                     /locus_tag="VW343_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15569..16198)
                     /locus_tag="VW343_001493"
     CDS             complement(15569..16198)
                     /locus_tag="VW343_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9756026.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(16208..17209)
                     /locus_tag="VW343_001494"
     CDS             complement(16208..17209)
                     /locus_tag="VW343_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9756027.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(17240..17881)
                     /locus_tag="VW343_001495"
     CDS             complement(17240..17881)
                     /locus_tag="VW343_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9756028.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(17900..18715)
                     /locus_tag="VW343_001496"
     CDS             complement(17900..18715)
                     /locus_tag="VW343_001496"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9756029.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         6063 a         2940 c         3896 g         5881 t
ORIGIN      
        1 atacctttta tgaggctctt ttgtcttgtt cttgtttcaa ttgactataa atcgctataa
       61 gaaaaaatca ttttattggt ataaagaagt gattgaaagt aatggcaaaa cgttagataa
      121 ttagttcata aaaaacaagt ttagatcctc atctaaactt gtttttttaa aattgaagga
      181 tgattttgga ggatataaga tgacattgac tattcatgta tattataaag gtgatggaga
      241 tagtgcaata aagtttgcaa gagaaatgct tgatagtggc cttgttgaag aaattagaaa
      301 tcaaaaaggt aacttgaaat acgaatattt tttaccaatt gaaaaagagg gaactattct
      361 ccttattgat cagtggataa atcaagaaac tttagataaa cattatcaat caaagacaat
      421 gcagaaaatc ttagatttaa gaaaaaaata tcacttgcag atgcaagttg aacgttatat
      481 agaagatgat tctgggatgc cagaaagcga taagtcgttt atagacacag ggaattagaa
      541 ttattgtgag agtttttgta gtttgaaagt attgaaaatc gtaaagatat cattacgaga
      601 atacaagaag atattgttct atttccatta aaaattactt ctttccttaa caataaggaa
      661 aaagaatacg ttttcatttt ttggtttgag gtttataata agagtatcaa agtcaggagg
      721 aaaaaagatg actactttta aagatagatt tttatggggt ggtgctgttg ctgctcatca
      781 acttgaaggc ggatggcaag aaggtggcaa aggaattagt gttgctgatg ttatgactgc
      841 tggtcgtcat ggagtagctc gtgaaattac ttcgggagtt ttagagggta aatattatcc
      901 aaatcatgag gcgatagatt tttatcatcg ttataaagaa gacatagcac tttttgctga
      961 aatgggattc aagtgcttcc gtacctctat tgcatggaca cgtatctttc caaaaggtga
     1021 tgagttagag ccgaatgaag aaggattaca gttttatgat aatctttttg atgaatgctt
     1081 aaagaatggt attgaacctg tcatcactct atctcatttt gaaatgcctt atcacttagt
     1141 gaccgaatat ggtggttgga aaaataggaa attgattgat ttctttgctc gttttgcaga
     1201 agtcgtattt aaacgttaca aagataaggt taaatattgg atgactttca atgaaatcaa
     1261 taatcaagcg aattatcagg aagattttgc accatttact aactcaggta ttgtatatga
     1321 ggaaggtgat aatagagaag caattatgta tcaagcagca cattacgaac tagttgcttc
     1381 tgcacgagct gtaaaaattg gtcatgagat taatccagat tttcaaatag gttgtatgat
     1441 tgcgatgtgt ccaatttatc cagctacttg caatcctaag gatatcttaa tggcaatgaa
     1501 agctatgcag aagcgttatt attttgctga tgtgcatgtt ttaggtaaat atcctgagca
     1561 tattttcaag tattgggaac gaaaaggtat ttcagttgat tttactgacc aggataaaga
     1621 agatttactt ggtgggactg tagattacat tggtttcagt tactatatgt cctttgctat
     1681 agactctcat cgtgaaaata atccttattt tgattatctt gaaacagaag atttagtgaa
     1741 aaataattat gttaaggctt ctgaatggga gtggcaaatt gatccagaag gtttacgtta
     1801 tgcgttaaat tggtttacag accactatca cttaccactc tttattgttg aaaatggttt
     1861 tggagctata gatcaagttg cagcagatgg tatggtacat gatgattata gaattgaata
     1921 tctaggtgcc catattcgtg aaatgaaaaa ggctgtagtt gaagatggtg ttgatttaat
     1981 gggttatact ccatggggat gtattgattt ggtttcagct ggtaccggtg aaatgcggaa
     2041 acgttatggt tttatttatg tagataaaga tgacaatggg aagggaagtt ataatcgttc
     2101 cccgaaaaaa tcttttggct ggtataagga agttatttca tctaacggtg aatcagtaga
     2161 atagtataaa caaactgaga ttattctcag tttgtttagc atattgggat gacatatgat
     2221 tggaagtatt gcagcaattt taacaacatt tgctttttac cacaagtttt tcgtatagtt
     2281 aagacaaagg acacaggatc tattgctcta ggtatgtatg ttatgcaagt tattgatatt
     2341 gcattgtggc ttgctcatgg tattcgtata ggagacttgc ctttgatttt agcaaatagt
     2401 gtttcatttc tactctcagg aataatttta ttttataaat tgaagtataa ataatttttt
     2461 tccatatcat ttaggaaaat gagggaattg tcattggaaa cgctttattt tataataaaa
     2521 atagataaca gataagtcaa cttagcacag ctttgattgg atttaagaaa aggagaaaat
     2581 attatggcaa aagctttaat tatttgtgca gcaggaatgt cttcatcact tatggcacag
     2641 aaaacaacag agtttctaaa aggaaaaggg aaagatattg tagttgatgc tattagtgct
     2701 actgaaggag ggaaagctat cgcagcagca gcttttgatt tgtatcttgt cagccctcaa
     2761 acaaaaatgt actttaagca atttgaagag gctggttcta aagcaggaaa accagttgta
     2821 caaatacctc ctcaagccta tattcctatt ccaatgggaa ttgaaaaaat ggctaatttg
     2881 attcttgaga atatttgatg aacacatgaa atgtcaataa atatttaagt taagataaat
     2941 tgttttgagt ttgatataaa ttgatttaag tgtataatat gcttcaatat atgaaaacat
     3001 acgttttagg agggaatgaa tgtgttaaac attaaagaga aagatatcct tcaattttta
     3061 ataaaaaata aggaacgttt tgttacaagc aaagaactgg cagaatattt atcctgttca
     3121 gatagaactg tccgtaatgt actgaaatta attgagaaga caatgattat tcaaggagtc
     3181 cggttgattt caaaacaagg tcaagggtat caaatatttt ttgagaatca aggcgcttac
     3241 caggagttta gacaaactta tgaactggaa gaagactata cgaaaactgc ggtttcaaaa
     3301 ggtgatgatc gtttagtatt tatcttaaat aaattattat ttgagcaagt tccagttctt
     3361 tttgatgatt tagctgatga actttacgtt agccgttcaa cattatctca tgatttcaga
     3421 aagatacgag tgatgttatc agagtataat ttgtccattg agagtagggc gaataaagga
     3481 gtttatgtct caggcgagga acgtgataaa cgacgtttta tcataaatta tttcctagag
     3541 aatcaatttt ttaaaacaat acattgctat gtaaaattta acttttttga tcaaactgtt
     3601 ccattagaag aatttgctag aattgttttg gatgaatgcc aagaggccaa cctaaaacta
     3661 tctgattttg tactacagaa cttagtagtt cacatagcat tgtctgtgat tcgtttaaaa
     3721 tccggatttg aaataaaaaa tatagactgt cagatgactg atgatgcaat agagagaaag
     3781 gttgcccaaa gaattctttc taaagtaaga gaagtaacaa atcaggaatt tccagttcag
     3841 gaaatagact acattacttt gcatttgtta gctaagagtc aacaatgtca aaaaaatcag
     3901 aagaatattt ctgaagaagt tttaaaaaaa tctttattta aaacatttca gaatttagga
     3961 ttagatgata tgtataactt ttcttcagat ttccaactaa ttgaaggatt gataactcat
     4021 ctaatgacac tacaagtacg tttagaaagc cggattacct taaataaccc tttggtagat
     4081 gaaattaaac agaattatag tgatatattc tttatgacta gagaaattct tgcaaatatg
     4141 gacaagttct tggagtggac aatatcagat gatgaagtag catatgtaag tcttcacttt
     4201 ttagctgcta tggaacgtag caaagagagt actaagttta atattcttgc tatttgtgca
     4261 acaggatttg gtgcagccca aatgttaaga aatcgtttgg aaacggagtt tgggaagcga
     4321 gtagaagttg ttgatgtaat aggctattat gaattgaatc aagaaaaact taaaggaata
     4381 gattttatag tgtcagctgt tgatttatca aatctatatt ttcaaatccc agtttttaaa
     4441 gttagtgtgt ttcttaagag tgatgagatg gaaatgatta gaaaagctat ggatcagatg
     4501 caagtttcta gttatgtaca atcatcaaag attaacaaat ttgaaaacaa cggctttaga
     4561 cagtatttta gtaaagaaaa ctttttaata tgtactgaat cagacaaggt aaatttactt
     4621 gaaaagatgg ttgagagttt atctgttggt gaatcaaatg agtttgaaca atctttactt
     4681 tatggaatta aacagcgcga agaattaagt tcagttgttt tttctgaaaa aattgcagtt
     4741 ccgcatccta ttcagccctt tggtacagag ggaaaggttt cggttgcaat ttgtaaagat
     4801 tcgttactct gggataatca aagttcacat gtccagctag tatttcttct atctccatca
     4861 atatatggga acgaaggttt ggcaactgtg actaaaaaga tagtctctct gacagagaat
     4921 gatgaattgc aaaatcagct aatatcttgc aataactttg aagattttat taacatattt
     4981 gagaagataa aataggaggt tcatatgaat caagaggaac tgcaggttgc agcatttgaa
     5041 attatcctgc attcgggaaa tgcaaggtca gaaatacatg aagcatttgc aaaaatgaga
     5101 gaaggtagtt ttgatgatgc agaaagtaaa ttaaatcaat caaatgagat aattcttgaa
     5161 gcgcatcatg cacaaaccaa actcttgcaa gaatacgcaa gtggagtgga aattaaaatt
     5221 gaaattatca tggtacatgc tcaagatcat ttaatgacca ctatgacatt attagaagta
     5281 gcaaaggaaa tgttagctct ttataagaaa gttaactaaa tatgtgttgt tatataagga
     5341 ggaaatgata tggataaaaa agaacgacaa aagattgaac aacaacgtcg agagatggct
     5401 ctaactaata ctttttttaa tcgatatctt cttctacgtt attcaatagc actcttcttt
     5461 tttgggaata tttattggct tttgaatcag tttattaatc cctcgcctat tattattttc
     5521 cccattatgc tcatagtatt ttctatttta gcaactgttg aacaatttaa actctatgga
     5581 aataggaaag aaaagttggg aataacacta atgtttgtta gaatacaaat gcttatttca
     5641 ataggattac ttgttttaac ttggacaagt tggtttaaga atctttttcc gatttttgaa
     5701 aataaccaag tagcacgttt atttgttttt gtagttcttc tattaggttt agttctgagt
     5761 ttgttagaca ttagaagaat taaaaaaatt tataaacgaa cagataaggc ttatcaacaa
     5821 tttgtccaat tggaaaagaa ctcacttagt ttataatcct gatctagtat aatttgtatt
     5881 tgaaactagt ctttagaaag taaaaattat tatgttttat taaggaggtc tatttatgga
     5941 tcaacaaaac gggttgtttg gttttcttga aaaccatgtt atgggaccaa tgggcaaact
     6001 tgctcagttt aaagtagtgc gtgctatcac ggctgcaggt atggctgctg taccatttac
     6061 tattgtagga tcaatgtttt tggtattcag tattttgcca caagctttct cattttggcc
     6121 aattgtggca gatattttct ctgcttcatt tgataaattc acatcacttt acatggttgc
     6181 aaactatgcg actatgggtt ctctatctct ttatttcgtt ctatcacttg catatgaatt
     6241 gacaaaaatt tatgcagagg aagaagaact caatatgaat cctcttaatg gtgccttgct
     6301 tgccttgatg gcttttgtca tgacagtacc gcaaatcatt tttgatggtg gaatgatgaa
     6361 gactgtgaca agtctaaaag aaggtgcagt aattgcagat ggatgggcaa tgggaaatgg
     6421 agtcgcacgt tttgggacaa cagggatttt taccgcaatc attatggcaa ttgtgactgt
     6481 tattatttat cgtatgtgtg ttaaacataa ttgggttatt aaaatgcctg aagctgttcc
     6541 agaaggagtt tctcgtggat ttaccgcttt ggttccggga tttgttgttg catttgttgt
     6601 tatctttatc aacggtcttc ttgtagcaat gggaacagat gtttttaaag tcattgcaat
     6661 tccatttggt tttgtatcca atctgactaa ttcgtggatt ggtttaatga ttatttattt
     6721 actgactcaa ctactttgga ttgtaggtat ccacggtgcg aacattgttt ttgcatttgt
     6781 tagtccaatt gctcttgcta acatggctga aaatgctgct ggcgggcact tcgctgttgc
     6841 aggtgaattt tctaatatgt ttgtaattgc aggtggttct ggtgcaactt taggactatg
     6901 tttatatatt gcttttgcct ctaaatctga acagcttaaa gcaataggac gagcatctgt
     6961 agttccagcc ttatttaata ttaatgaacc attaattttt ggattaccta ttatctataa
     7021 tccagccttg gctataccat ttattttagc accaatggtt actgctacta tttattacgt
     7081 agcgaattct ctaaacttta ttaagccaat tatcgcacag gttccatggc caactccagt
     7141 agggattgga gctttcttag ggacagcaga tcttcgagct gtattagttg ctctagtatg
     7201 tgcatttgca gcattcctag tctatcttcc attcatccgt gtatatgatc aaaaattggt
     7261 gaaagaagag caaggtatct aataagtaaa aaagtatact cgatgataga atccatatag
     7321 tttaaataat gagcgttaac attagcagtg aagaggtctt tttaagatat tcttctctgc
     7381 tttttcatat tttttcgcta tgtagggtat gaagcaaaat agaaagtagt tcgaatagtt
     7441 gataaacagg gaactgctgt tattttaaaa aaacaataaa gtaaagtatg ttatcgtaga
     7501 atacgataca atagtaaaaa agcaaaaaga atgcgtttta tatttgggtt tgaggcttat
     7561 aaaataggat aagcatgctg aaaacttaga tatgacaaac aaaataaaat ttttaaaagc
     7621 tacatagaaa catggtataa tgtatctaaa ggaggtagca tatgtcctta gatatagata
     7681 aagaaaagat gacaatcatg ggaatagctt ttgaaaaccg atccgttttt aagagtgttt
     7741 ggtatgcttt aagtacaaat atgattgaag gatggcgacc tacggttagt gatgttgaaa
     7801 aattacgaga tgaagctctt gctctgggga tgacttaatg aagcgcaact gttatgaatc
     7861 ttatgactat gttgatccgg ataatttata taccgatccg ggttcttcag ttctaagaaa
     7921 taagcaggaa ggaaaaaagc acgtgagctt gaatattgta tgattgctag taagagtttg
     7981 aaattattta tcaatcctat cttagttaat agtttaaaag tagtttgttt cacaactgca
     8041 taagcttaga aaagttgaaa agtgataact ctttaataaa ctcttggttt gttaaaagat
     8101 attttgtcca ctacattttt tcaattgact attatttgtc atagatataa cagatagaac
     8161 attgcattca gaataatgaa aaacgcatca aaaatggtgc gttctgtttt tatgctacca
     8221 taaaaggagg gaaacaaatt gaaaattttt aagggagagt tttatcgcat ctctgtatta
     8281 acagacaagc tagtaaggtt agaatactct aaaactggca gttttgagga tagaactaca
     8341 caacttatct ataatagaga ttttggccaa gtttcgttag attatatcga gacatcaaac
     8401 gtactagata ttatgacgga ctattttcat ctgcacttta ataaaggaga atttaacgcc
     8461 gaaaatttat ttatagaatt aaaaggaaat tttgccgtat atggtagtcg ctggtatttt
     8521 ggtgaatcga ttgaaacgtt aaaaggaaca gctcggactc tggataaggc agatggagca
     8581 atcccattag aagatggaat tattagtcga aatggtatag ccttattgga tgattctcaa
     8641 ggatttattt gggatgaaca gtctggttat attgagagag aaaatcaaat tgacctctat
     8701 ttctttgcct atgggcatga ttatagagga gcaatcagag acttttacca tttgactggt
     8761 tcaacaccct tgttaccaag atatgcttta gggaattggt ggagtagata ttggccttat
     8821 acgtcggatg aatacttgga tttaatagac agatttgaaa cagagaaaat tccattatct
     8881 atcggtgtgt tagatatgga ttggcatata actgacattc cagctcgttt tggaagtggc
     8941 tggacaggat atagttggaa taaaaactta atacctaatc cagaacagtt attgcaacaa
     9001 cttcatgata gaaagttaaa actctcttta aatgtccatc ctgctgatgg tatacgggct
     9061 tatgaagaag cttatcctca agtcgccaaa cgattagggt taaatgtaga actagaagaa
     9121 cctgccattt ttgatttttt taatccctct tttagggaag cctactttaa agatgttcat
     9181 tatgagctag aaaagcaggg agtagatttt tggtggattg actggcaaca agggactcaa
     9241 ggcatattgg atccactatg gcttttaaac cattatcatt atcaggatag ttgtaaaaat
     9301 gcagaaggtg gtttgatttt atcaagatat gcaggtcctg gtagccaccg ctaccctgtt
     9361 ggtttttcag gggacactat tattagttgg aattccttaa gatttcaacc ttattttaca
     9421 gcgacagcat ctaatatcgg ttatagttgg tggagccatg atatcggtgg gcacatgctg
     9481 ggagattatg acgaagagct gcaaactaga tggctacagt ttggtgtttt tagtccgatc
     9541 actcgattac atagttctag aagtcctttt aatagtaaag aaccttggtt tttttcagaa
     9601 acaacatcta agattatgaa gaaatacctt cgtttgaggc atcagatgat tccatatcta
     9661 tacactatga atgtaaagac acacgaggaa ggtgccccat taatcagccc aatgtattat
     9721 ttctacccag agaatgatga gagctataat gttccaaacc aatacttttt tggaacagaa
     9781 ctaatggtgg ctcccattgt agaaaagatg gacttggcat tccaatctgc aaaagtggat
     9841 gtatggttcc ctgaaggtga atggtatgac ttcttttcag agaaaaaata tacaggtggt
     9901 gtgaagttga gtgtttatag agacatttcg actatacctg tgtttgcaaa aagtggtgcg
     9961 atcattccct tggttggttc tgagatagat atgggtgttg atttacctga agttgtagat
    10021 tggtatgtat tcccaggcaa acaacattct tttgaaatgc ttgaagatca aaatggtcaa
    10081 agatataaaa caagattatc aatcgattgg gaaatgggga tggtagagtt aacattacaa
    10141 ggagattcta gtatcgttcc aagcaataga aggcatagaa ttcattttaa aggaacgaat
    10201 gtgtctataa ttgaattacc aaataagaat gatacagcta gatttgaatg taaagataat
    10261 aaaacaatat ctttaaatga tgaagttttt agactactaa agacagcttc tcttccatat
    10321 gaattaaaag atagattgtt aaatcaattc atcaatgcga aaaactctca tgacttaatg
    10381 aatatcttgc atcatcagga taaggaattg agagggcgtt tgttggaaat gatatttact
    10441 agccaaaact aattgatata attagaaagt catttgttta gtgtatatga agattctgta
    10501 tggtattcgt tatcactggt cacattgaaa atgtgaaata agcacctaca acatagactt
    10561 tacaacactt catgtttcat atcatggggt gtttttctat ttaagtcaaa gccaatcaaa
    10621 aaatctcact attcatcagg ttaaaggaaa cctaatcata gtgagattgt gaattatagt
    10681 agagtttgta tcgcctcttc aatttgtttt gggtctgttt ttgaagaaaa gcgttcaaag
    10741 acttgcccat ctcgaccgat gagaaactta gcgaaattcc attcgactcg ttttcctagt
    10801 gggccggatt tctggtcttt taaccagaca tagagagggt ctgcttcctt accgttgacc
    10861 ttaatcttgg caaaacgtgg gaaggtggtt tgaaaatgta ggctacagaa ggcgttgatt
    10921 tcctctgcgc tgccgggtgc ttgtcccata aactgattgc aagggaaatc caatatttca
    10981 aagccctgat cttgatagcg ttcatagagt tcttgaagtc cctggtactg gggcgttaaa
    11041 ccacatccag tagcagtgtt gacaatcaag agaactttgc cacgatagct atccaaggga
    11101 gttgcttggt tgttttggtt caaaacggaa aaatcataaa gtgaagtcat gagggcctct
    11161 tttctttttc tacattgtaa cagtttttac ttttttcgtc gagtcggatg gaagatattg
    11221 ttgaagaata agccctcaat atgatagaaa ccgtttacaa ataactttaa aaatggtata
    11281 atttaattaa accggtttac ttagttgcta taaatctcta atttgagatt tagttcctat
    11341 tgattttaca atatgtttat tggagtgtat acatgcaaac aaaaacaaag aagctcattg
    11401 tgagtttgtc ttcacttgtt ttatcaggat ttttattaaa ccattatatg acagttggag
    11461 cggaagaaac gactacgaat accattcagc aaagccagaa ggaagttcag tatcagcaaa
    11521 gggatacaaa aaatttagtt gaaaatggtg attttggtca gacggaggac ggaagcagtc
    11581 cgtggacagg aagcaaagct caggggtggt cagcttgggt agaccagaag aatagttctg
    11641 cagatgcctc aactcgagtc attgaggcta aggatggggc tatcactatc tcaagccctg
    11701 agaaattaag ggcagcggtt caccgtatgg ttcctattga agctaagaaa aagtataaac
    11761 tgcgtttcaa gattaaaaca gatcataaag tcgggattgc caaagttcgt atcattgagg
    11821 aaagtggtaa ggacaagcga ttgtggaatt ctgcaacgac gtcaggaaca aaggactggc
    11881 agaccattga agcagactat agcccgactt tagatgttga taaaatcaag ctggagttat
    11941 tctatgaaac aggaactggg actgtttcct ttaaggatat tgagctggta gaggtagcag
    12001 cccagctttc tgaggattct caaacagata aacagcttga ggaaaagatt gatttaccaa
    12061 ttggaaaaaa acatgttttt tctcttgcgg actatactta taaggtagaa aatcctgacg
    12121 ttgcttcagt caaaaatgga attttagaac ctcttaagga agggacaacc aatgtcattg
    12181 tcagtaaaga tggcaaggaa gtgaaaaaga ttcctttgaa gattctagcc tctgttaagg
    12241 atgcatacac agaccgtttg gatgactgga atggcatcat tgctgggaat caatactatg
    12301 attctaaaaa tgaacagatg gccaaattaa accaggaatt ggaaggaaag gtagctgata
    12361 gcctatctag tatttcaagt caggcggacc gcacctattt gtgggaaaaa ttttcaaatt
    12421 ataaaatgtc tgcaaatctg actgccactt atcggaaatt ggaggagatg gccaagcaag
    12481 tgaccaatcc ttcttctcgt tattatcaag atgaaactgt cgttcgaaca gtcagggatt
    12541 ccatggaatg gatgcataaa catgtctaca atagtgaaaa gagcattgtt gggaactggt
    12601 gggattatga aatcggtaca cctcgtgcca tcaacaatac cttgtctctg atgaaagaat
    12661 acttctctga tgaggaaatt aaaaaatata cagatgtgat tgaaaaattt gtaccagatc
    12721 ccgaacattt ccgaaagacg actgataacc catttaaggc tctaggtgga aacttagttg
    12781 atatgggaag agtaaaagta atagctggtt tactgcgtaa ggatgatcaa gaaatttctt
    12841 ctaccattcg ctcgattgag caagtgttca agttggtaga ccaaggtgaa ggtttttatc
    12901 aagatggatc ctatatcgac cataccaatg ttgcctatac gggtgcttat gggaatgttt
    12961 tgattgatgg cctgtctcaa ctgttgccag tcattcaaaa gaccaagaat ccaatcgata
    13021 aagataaaat gcaaaccatg taccactgga ttgataaatc gtttgctcct ttgctggtga
    13081 atggagagct gatggatatg agtcgtggac gctcgatcag tcgtgcaaat agcgaggggc
    13141 acgtggccgc agtagaagta ctaagaggga ttcaccgaat agcggatatg tctgaaggag
    13201 aaaccaaaca acgtttgcag agtcttgtga agaccattgt tcaatcggat agttattatg
    13261 atgtctttaa gaatttgaag acttataagg atatcagttt gatgcaatcc ttgttaagtg
    13321 atgcaggagt cgcaagtgtt ccaagaacaa gttacctatc tgcctttaac aagatggata
    13381 aaacagccat gtacaatgca gagaaagggt ttggatttgg cttgtcactc ttttccagtc
    13441 gtaccttgaa ttacgaacac atgaacaagg aaaataaaca tggttggtat acgagtgatg
    13501 ggatgttcta tctttacaat ggcgatttga gtcactatag cgatggctac tggccaacag
    13561 ttaatccata taagatgcct ggtacaacag agacggatgc taagagagcg gatagcgata
    13621 caggtaaagt tctaccgtct gctttcgttg gaacgagcaa actagatgat gccaatgcga
    13681 cagcaaccat ggatttcacc aactggaatc aagtaatcca tataaagtct atgtcaatga
    13741 taaagaagcc tcccttacag aacaagaaaa ggattatcct gaaacccaaa gtgtcttttt
    13801 agaatcgtcc gattcgaaaa agaatattgg ttactttttc tttaagaaga gttcaatcag
    13861 tatgagtaag gctttgcaaa agggagcctg gaaggatatc aatgaaggac agtcagacaa
    13921 ggaagttgaa aatgaatttc ttacgattag tcaggctcat aagcaaaatg gagattctta
    13981 tggctatatg ctaattccta acgtggatcg tgccaccttc aatcaaatga taaaagagtt
    14041 agaaagtagc ctcatcgaaa atagcgaaac ccttcagtct gtttatgatg ctaaacaagg
    14101 agtttggggc attgtgaaat atgatgattc tgtctctact atttccaacc aattccaagt
    14161 tttgaaacgt ggagtctata ccattcgaaa agaaggggat gaatataaga ttgcctacta
    14221 taatcctgaa acccaggaat cagctccaga tcaggaagtc tttaaaaagc tagagcaagc
    14281 agctcagcca caagtacaga attcaaaaga aaaggaaaaa tctgaagagg aaaagaacca
    14341 ttcggatcaa aagaatctcc ctcagacagg agaaggtcag tcaatcttgg caagtctagg
    14401 gttcttgcta cttggggcat tttatctatt ccgtagagga aagaacaact aatttgttca
    14461 tcatctagat gaattacatg aaactgtgga aaatagaaaa aatccgaaga aggccaaata
    14521 tcttcggatt tttcttttaa aaaaggataa gttgatactt atcctttttt acttcattat
    14581 tatgtgacta tagcgttttg aaactagaat agaacgttat aactgctaaa agatttctat
    14641 aaattcattt gattttccta atcaatttgt tcgtatccta tttcactcca ctatataagt
    14701 ccgtctcgct ccgtgaggtg cgcgtcgaag gttataccaa aaaaactcca aacgcgatac
    14761 aataaaggta ttcaatccaa ttgtaaagcg aaaggagaaa aaatatggca caaaagactc
    14821 atagtttatc atacacaaag tggatgtgtt ctatcatatt gtgttcaccc ctaagtatag
    14881 acgaaaagtc atctataatc aatatagaag tagtttaggc gaaatatttc atcgcttgtg
    14941 tagttataaa ggtgttgaaa tgatcgaggg tcacttaatg ccagaccatg tacacatgtt
    15001 agtcagtatt ccgtcgaggc taagtgtttc aagttttatg gggtatttaa aaggcaaaag
    15061 tgcactcatg atgtttgaca aacacgccaa tctcaagtac aagtttggga atcggaattt
    15121 ctgggcggaa ggttattatg taagtccagt agggcttaat gaagccacaa ttaagaaata
    15181 tagtcaagat taaagaaatt atcgagtgga tgatttcttc acgagtatga aaatttgaga
    15241 actagtaaag tatgatatat agtaaaatga aataagaaca ggacaaaatc gatcaggaca
    15301 gtcaaatcga tttttaacaa tgttttagaa gtagaggtat actattccag tttcaatcta
    15361 ctatagcact agataaattg agtgtaaaag aatatgagga tccctttagg gatagtggta
    15421 agtaatacca aagtctctta aagaggcaag tgacgagtca agagcaataa ggcttgaaca
    15481 acgtgaaagc cagcgtcttt aggcgctggc tgatgatttg ggcttatagc tctgagataa
    15541 accacccgtt agacgggtag ttatgatttt atctgagtgt aatatactgt tgggcaatct
    15601 cgctgatgcg gtcaaagttg ccttgggaag cgagtttatt gagttcgcca ccaattccaa
    15661 cggcatctgc accagcagcg aaccattgag ggatgttgtt taggccgact cctccggtta
    15721 ccattacgga aacttgtggg atcggtgcct tgactgcaga gatatatgct ggactgagag
    15781 tactacctgg gaagagtttg atgatttcac taccggcttc aagtgcagtc gtgatctctg
    15841 tgagggtaat acagcctgga atgtacggtg tgctgtagag attgcacatt ttcgcagttt
    15901 cagcatggaa agatggagaa acaacgtaat ttgctccagc tagaatggca tctctagcag
    15961 ttacggcatc aagcacagta cctgcaccga tacaaacact ctgatcgtcc tgatacaagt
    16021 ctacaagttc cttgatgatt tgtcctgcat actgattggt ataggcgatt tcaatagctt
    16081 tgataccgcc cttgatacaa gcaatcgagg cttgtagtcc ttcttccttt gtatttcctc
    16141 gaatgacagc gacaattttc tgttttttta gttcaataat cgtatctgat ttggtcatgt
    16201 aattctccta acgaatgata tcttgtgcat ttgccagtaa attttcaata ctagttgcgg
    16261 aagtggagag atggtctcct ggaagagtgc atttgagagt tgcgctcgca actgcaaagt
    16321 caatggtagt ttttagggag gaatgatgga gtagttggta aagggcacca gatataaagg
    16381 catccccgct accaattcgt tgatagactg cagtttttag ttggacagac tcttcaaatc
    16441 gttcttccag agcataggct tgatagacat ttttgtcttg ctcatcacta gatcggaggg
    16501 tatggaaaat ggccttgaaa ccataggctt cttttaaaag tcgcatgcga ttttccacct
    16561 cttctaggct agcactgtct cttggaaaca tctctagatt ttggtcatca atcatgagag
    16621 gatcaatacc gaagcaatag tcagtaaaac gtgcaaactt agaaaattca tacttggctt
    16681 ctaggactga aatcatcttt gttctcagat tgagatccat tgaaacgaca attcctcggc
    16741 gcttggcttc ttccaagagt aggagaagga tcgcacggac ctcttgaccg atagctatgg
    16801 tgattccact aaaatgaaaa tggctaatcc cctgaaagag agaatccata tctagcatgt
    16861 ttggccgaat ctggctgata ctcgtatgct tacgatcgta gaaaacttca ctttgacgac
    16921 aaccaaagcc gttctccaaa tagtagaggc cgattcgatc gccaagccga cagattgaac
    16981 tggtatcgat ttggtgctgt ttcaagaatg tgagaaaacg atctccaatc tcgttggcag
    17041 ggagtgcggt aaagactttc gttgagatac ccagggcttg caaattacaa gcgatgttaa
    17101 tttctgatcc gccaaaataa gtcgaactgg caacatgatc gccgatactg gtggcatcta
    17161 atggtgagat tcgaattaat ggttcgccaa acagtaatat tttcgtcatc cattagttcc
    17221 tttctggttt agaatagctt tacttggcta atacttcttt caaataagca gcgacttcat
    17281 catcttggca gttttcaaag aagtattctt ggaagtattt gccagagatg gtgtctttga
    17341 ggaagtcttg gtcgattgtt ttaaggatgg tcatcaaatc attgtgggtg atttgtttca
    17401 cctcgtttaa gatacgagcg ttacgttgtt cagggattac acgttctctt gggtagccac
    17461 cgcccatttc ttcagcaaat aagcgttcaa acatcaattt gagggtcagt tctgcccccc
    17521 agccaaatcc tttggcataa gggatagaca aggcgttacc accattgatt tgagaataaa
    17581 ggtaagcgtc agttgggtcc actgctagac cacagacaac accagggaag ctgtttaaag
    17641 caagcatagc ccctacaccc gtaccacagc cggtaacaac aaagtcaact gcctttgtat
    17701 ttaaaaggat ggcagccatt agtccgttct gcacataagt taattgactt tctccttctt
    17761 ctccacgcat accatagtta aataattggt agtctttttt atctgtcgct tcttttagac
    17821 tatcgtaaat aatgtgattc ttgctagctt gactattttc attgattaat gcgattttca
    17881 taagatcttc tttctatttt tactcaggtt gttttccgat gtaggctaag ataccgccat
    17941 ctacatatag gatgtggcca ttgacaaaat tgctggcatc actagcgaga aagacagcag
    18001 ggcccatcaa atcttcagga tttccccaac gtgcagcagg tgtttttgca atgatgaact
    18061 ggtcaaatgg gtggcgagaa ccatcttctt gcaactcacg aagaggtgct gtttgaggag
    18121 tggcaatata acccggtcca attccgttac attggatatt ggctccaccg tattcagacg
    18181 caatgttgcg ggtcaacatt ttcaagcccc ctttagcagc agcataagcg ctaactgttt
    18241 cacgtcccag ttcgctcatc atcgaacaaa tattgataat ctttccatgc cctttcttta
    18301 tcatagaagg aataactgcc tttgaaacga taaatggtgc gtttaagtca atatcgatga
    18361 ccttacggaa atcagcggcg ctcatttcgc acattggaac tcggcggata ataccagcgt
    18421 tattaacgag gatgtcaatg acaccaacct cttgttcgat ttgcttgacc atggcttgga
    18481 taccgtcctc gtctgtcacg tcacagacat atccatgggc ttcgatgcca acttcacgat
    18541 aagcttcaat ccctttattg accagatctt gattgatatc gttaaagaca atagtagcgc
    18601 cggcttcagc gtaggatttg gcaatagcaa atccaattcc ataagaagcg ccggtgatga
    18661 gagcaatttt tccttttaaa gaaaactgct caattgagaa tgatgtattt gtcatttgat
    18721 acttcctttc aaatttgtta agacttctct ataaccaaat tatattctag taaaccggtt
//
LOCUS       DAVKMU010000036        18673 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKMU010000036 DAVKMU010000000
VERSION     DAVKMU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18673
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.036"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(171..977)
                     /gene="yidA"
                     /locus_tag="VW343_001497"
     CDS             complement(171..977)
                     /gene="yidA"
                     /locus_tag="VW343_001497"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9756030.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMADKEIVKCMFIDEPE
                     ILDATIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(990..2294)
                     /locus_tag="VW343_001498"
     CDS             complement(990..2294)
                     /locus_tag="VW343_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9756031.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2366..2743
                     /locus_tag="VW343_001499"
     CDS             2366..2743
                     /locus_tag="VW343_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9756032.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDGQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            2833..3165
                     /locus_tag="VW343_001500"
     CDS             2833..3165
                     /locus_tag="VW343_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9756033.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            3177..4748
                     /gene="ffh"
                     /locus_tag="VW343_001501"
     CDS             3177..4748
                     /gene="ffh"
                     /locus_tag="VW343_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9756034.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(4943..6226)
                     /locus_tag="VW343_001502"
     CDS             complement(4943..6226)
                     /locus_tag="VW343_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9756035.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMLATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTSGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6480..7193)
                     /gene="rsmG"
                     /locus_tag="VW343_001503"
     CDS             complement(6480..7193)
                     /gene="rsmG"
                     /locus_tag="VW343_001503"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9756036.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7287..7847
                     /locus_tag="VW343_001504"
     CDS             7287..7847
                     /locus_tag="VW343_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9756037.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7849..8748
                     /gene="htpX"
                     /locus_tag="VW343_001505"
     CDS             7849..8748
                     /gene="htpX"
                     /locus_tag="VW343_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9756038.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8897..10456)
                     /locus_tag="VW343_001506"
     CDS             complement(8897..10456)
                     /locus_tag="VW343_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9756039.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEICSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10695..11012)
                     /locus_tag="VW343_001507"
     CDS             complement(10695..11012)
                     /locus_tag="VW343_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9756040.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            11182..11724
                     /locus_tag="VW343_001508"
     CDS             11182..11724
                     /locus_tag="VW343_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9756041.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11724..12353
                     /gene="nth"
                     /locus_tag="VW343_001509"
     CDS             11724..12353
                     /gene="nth"
                     /locus_tag="VW343_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9756042.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12564..13085
                     /gene="pyrR"
                     /locus_tag="VW343_001510"
     CDS             12564..13085
                     /gene="pyrR"
                     /locus_tag="VW343_001510"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9756043.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13104..14027
                     /locus_tag="VW343_001511"
     CDS             13104..14027
                     /locus_tag="VW343_001511"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9756044.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14077..15156
                     /gene="carA"
                     /locus_tag="VW343_001512"
     CDS             14077..15156
                     /gene="carA"
                     /locus_tag="VW343_001512"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9756045.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15469..18645
                     /gene="carB"
                     /locus_tag="VW343_001513"
     CDS             15469..18645
                     /gene="carB"
                     /locus_tag="VW343_001513"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9756046.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIKSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5422 a         3614 c         4188 g         5449 t
ORIGIN      
        1 gagtgattta ttacaattca gtaccacaaa ttaagaaagc aatgcctatt ttttgtactc
       61 tcaagattta agtaactgta caactgcaat taaatttatt aatttactct cattgtgata
      121 tattcataca aaaaccgctc agctgagcgg ttttagtcta ttctttcctt ttacagtacc
      181 catgttcgga tggcgtgggc aacgccggat tcgtcatttg ttttagtgat gtatttggcg
      241 atttttttga tttctggatt tccattttcc atgacaacgg ggtttccaac gacttccagc
      301 atggcacggt cattttcttc gtcaccgatt gccatggttt catctttggt caatccgagt
      361 ttttcagcca agtgagtaat ggctgaaccc ttgtctacat tctttgtaag gagttcgagg
      421 tagaaaggag cagatttgtt gatggagtag cgctcgtaaa attctgctgg aattttttca
      481 atcgtagcat cgaggatttc tggttcatcg atgaacatac atttgacaat ttccttgtca
      541 gccatttctt caggtgtacg gtagaagata ggcatgctga cgagggttga ttcgtgcaca
      601 gtgtattttc cgatattgcg attggcagta tagataccgt ccttggtaat ggcgtgcatg
      661 tggacaccga gcttgcggct gagaaattcc atatctagat aatcctcata agtcaaggat
      721 tcgctgataa tctcatgtcc tgtagcagtt tcttggacaa gggcaccgtt gaaggttacc
      781 acatagtccc cctcgtctct caactgcaag tcgtctagaa gtttggcaac gcctgcgata
      841 gggcggccag ttgcaatcac gactttgaca ccagcttctt tggcatcttg gatggcagaa
      901 aagacttcag gagtgatttc cttttggctg ttgactaggg ttccgtcaat atcgacggcg
      961 attagtttaa tactcataaa tttcctcttt tagtttttat ttggggtaaa atgatcgttc
     1021 tcaatcaagt gtaaaaattg ctgggtgatg cttgcgaaga tgctgttttg gtctaacatt
     1081 tctttcggga aataaaagcg attatctccg tggcgacttc cagcaaggga ttggacgata
     1141 ggggacaggc tagagagttc tgccagttct ccattttttt gtaaaatctc aatctgtgtc
     1201 cttggatttt cagattcggg acggtaaata tcataaggga ggtcaaagtt cttatgaatg
     1261 gcagtatagt agtcgggatc aaagccgatg tcctcaacca attttctcat gctagcgagt
     1321 tggtcttggt cttcttgtga aaaggtaatg gatttaaaga ccttgcggtt gacaaagcga
     1381 tgcgataaat ctgcaagaat cttgtcagga ctggtcatcc agagttggaa gtaggtattc
     1441 atcacaccat catccagagc cagatagtca gtcaaggtca catttttttc gaagaaaggc
     1501 aggaggtgtg gagaagttcg ggcaaagaaa tccttgtcct caggatagag ttccttggcg
     1561 cgtttgagaa gattctgtag gagaacttcc atggcgcgtg ttgcggggtg gaaataaacc
     1621 tgcatgtaca tctggtagcg actgaggacg tagtcttcga tggcgtgcat gccattgcgc
     1681 tgaaaggcga taccattttc gataggacga atgactcgga ggattcgagt caggtcaaat
     1741 tccccatagg atgctcctgt aaaataggag tcgcgcaaga gatagtccat gcgatctgcg
     1801 tcaatctgac tagaaatgag ctgcacgacc tgcttgttag gataggtatg gtctatgaca
     1861 ctggcaactt tctctggaaa atcgggcgct acttgtagca ggacttggtg aatctctgtc
     1921 tcaggatttt gaataatctc ctgagtaatg gcttcatggt ctgtatcaaa gagattttca
     1981 aaagtatggg agtaggcacc atgcccaagg tcgtgtagga gagcagcggt catggtcaag
     2041 agagactcgg caggattcca ttcctcagga tatttttctt cgaaaatctc tgtgatgcgt
     2101 cgtgcaattt catagactcc tagacagtga gagaagcgac tgtgttctcc accgtggaag
     2161 gtataactgg aagttcccag ttgtttgatc cggcgcaaac gctgaaattc ttttgtatta
     2221 atcaagtcat agatgatttg attattgaca tggatgtagt tgtgaacagg gtcacggaat
     2281 actttttcgt tcatatgacc attatagcaa aatcctgctc tttttggtaa aatagtagga
     2341 caagagacaa gaaagagggc ctgatgtgaa gattcggatg cgaaatacaa ttcagtttga
     2401 tgagcagttg gaagtgattg accagcttta tgatgtggaa gtgcatgaaa aaggagatta
     2461 tagctacctg cttttctata atgaggaaaa ggaaaaagta gtgattaaat ttcatggtca
     2521 agaacttatg atgagccgat tttccaatcc caagaccatt atgcgctttt taaaggatag
     2581 tgatagtttg gcctatattc ctacccctat gggcatgcag gaatttatca ttcaaacgag
     2641 ccgttatcaa gtagatgggc aaaagattga gctagcttat caactacaaa atcaagaggg
     2701 gcatcccttt gccagctatc aattggaaat tacttggggc taggatctcg tttttctcaa
     2761 atctactttc tatcttcaag gattggctcc ctagtgtggt agattaggct attttttggt
     2821 ataataaaag ttatggaaat cgaaaaaacc aatcgtatga atgcgctctt tgaattttat
     2881 gcggcgcttt tgacagataa gcaaatgaat tatatcgagc tttactacgc tgatgattac
     2941 agtcttgctg agatagctga ggagtttggt gttagtcgtc aggctgtcta tgacaatatc
     3001 aagcgaacag aaaagattct ggaagattat gagatgaaat tgcacatgta ctcggactac
     3061 attgtccgta gtcagatttt tgaccaaatc ttggagcgct atcccaagga tgattttctg
     3121 caggagcaga tagaaatttt aacaagcatt gataatagag aataagagga agaaaaatgg
     3181 catttgaaag tttaacagaa cgtttgcaga acgtctttaa aaatctacgt aaaaaaggaa
     3241 aaatctctga atctgatgtc caagaggcaa ccaaagaaat tcgcttggcc ttgctcgagg
     3301 ccgacgttgc cttgcctgtt gtaaaggact ttatcaagaa agttcgtgag cgtgcagtcg
     3361 ggcatgaggt cattgataca cttaatcctg cgcaacagat tattaaaatc gttgatgagg
     3421 aactgacagc cgttttaggt tctgatacgg cagaaattat caagtcacct aagattccaa
     3481 ccatcatcat gatggttggt ttacaagggg ctggtaaaac aacctttgct ggtaaattgg
     3541 ccaacaaact caagaaagaa gaaaatgctc gtcctttgat gattgcggcg gatatttatc
     3601 gtccagctgc cattgaccag cttaagacct tgggacaaca gattgatgtg cctgtctttg
     3661 cacttggaac agaagtacca gctgttgaga ttgtacgtca aggtttggag caagcccaaa
     3721 ctaatcataa cgactatgtc ttgattgata ctgcgggtcg tttgcagatt gatgagctcc
     3781 tcatgaatga gcttcgtgat gtgaaagcat tggctcaacc aaatgaaatc ttgcttgtcg
     3841 ttgatgctat gattggtcag gaagcagcca atgttgcgcg tgagtttaat gctcagttgg
     3901 aagtgactgg ggtcatcctt accaagattg atggcgatac tcgtggtggt gctgctctgt
     3961 ctgttcgtca cattactgga aaaccaatca agttcactgg tacaggtgaa aagattacgg
     4021 acattgaaac cttccaccca gaccgcatgt ctagccgtat ccttggtatg ggggatatgc
     4081 tcactttgat tgagaaagct tctcaggaat acgatgaaca aaaagccctt gaaatggctg
     4141 agaagatgcg cgaaaacacc tttgatttta atgatttcat cgatcaatta gatcaggtgc
     4201 aaaatatggg gccgatggaa gacttgctca agatgattcc aggtatggcc aacaatccag
     4261 cccttcaaaa catgaaggtg gatgaacgcc agattgctcg taaacgtgcc attgtgtctt
     4321 cgatgacacc tgaagagcgt gaaaacccag atttgttaaa tccaagccgt cgccgtcgta
     4381 ttgctgctgg ttctggaaat acattcgtcg aagtcaataa attcatcaag gactttaacc
     4441 aggctaaaca gctcatgcag ggtgttatgt ctggggatat gaataaaatg atgaagcaaa
     4501 tggggattaa tccaaataac cttcctaaaa atatgccaaa tatgggagga atggatatgt
     4561 ctgcccttga aggaatgatg ggacaaggcg gtatgcctga cttatcagct ctcggaggag
     4621 caggaatgcc agatatgagc cagatgtttg gtggcggttt gaaaggtaaa attggtgaat
     4681 ttgccatgaa acagtccatg aaacgtatgg ctaacaaaat gaagaaagcg aagaagaaac
     4741 gcaagtaaga caagagattg atgcaaatct catttgatga gaaaataaag taaaaatttc
     4801 tatgataaaa cgtacagtat caaggtttta gaatatctga tattatacgt tttttgattt
     4861 taataatttt gttaagacac ttttattata gtatacgaaa aatccactca gctgtctgag
     4921 tggattaggt gtatttagac tcttagtctt tattttcgta tggcaagatc aagttcaaga
     4981 tgattcctgt catggctgaa agggcagtac ctgaaagtgt aactggacca agtttaagga
     5041 tagctcctcc aagtccaaga accaacatag cacttgcgat gatgaggttt cgcatttgag
     5101 cgaaatcaac acgttcttta atcaagactt tcaaaccatt gctggcgata accccataga
     5161 gaaggattga cataccacca agtacagcgt ttggaatagt tgaaatcaag gcagtgaatt
     5221 taccaaggaa gctgagggca atcgcgatga aggcagcgtt acggataact gagacagaag
     5281 cgatacgagt cataccgata acccctgtat tttctccgta agttgtattg gctggtccac
     5341 caaggaaggc agaaacagaa gttgcgatac cgtcaccaat aagagtacgg tgaagacctg
     5401 gttcttttaa gaattgacgg ccacagattt gacccaaaac agtatggtct ccgatatgtt
     5461 cagaaattgt tacgatagcg attggcaaga tagcgatggc ttctggacca aagtaaagat
     5521 tgtactcttt aaaggcacca cttgtgctaa atggcaagta gaaaccagga atttcaaacc
     5581 agttagcttc taggacaggc gtaaagtcaa ccaaaccaag agttagtgcg aagaggtaac
     5641 caccgataat ggcaaagagg aatggaatga ttcgtaggaa gccttttcct tttgtattga
     5701 taaaggcagc aattaggaaa gtaacaacgg ctaccagagc atttttccaa tttccgtctg
     5761 ctacaagacc tgcattggta acagctgaac ctgcaagtcc aagaccgata acgatgatca
     5821 taggaccgat aatgattggt ggcaagagtt tatcaatcca ttttgttcct acaaatcgga
     5881 tgctggtagc aacaaggaca tagaccaaac cagtcaagat aacccctgtt tgagcagcag
     5941 atacatcccc ccccatttct ttcatagcca gtgacatagc tgtgataaag gcgaatgaag
     6001 aacctagata aactggaact ttaaaaccag tagcaagcat gtagatgagt gttccaacac
     6061 ctgaagcaaa aagggcaaca gatacaggca ttcccaaaat caatggtacc aagatggtcg
     6121 caccaaacat ggcgaaaacg tgttggaaac taaggagaat tccttttcca gccgaaggac
     6181 gttggtcaac gtctagtaac aaatcaacag ttgattcttg tttcatataa acctcacttt
     6241 tgggcatcaa aaaagagccc attattttac agcaataggc cctcagagta agacttcttt
     6301 aaaatttaga ttttaaagag ttttaaatat ttctgcgtct tcgtcacctc acgggatgac
     6361 attaaaaacc tttgcttagg atagtatagc agaaaaaaat gattttgtaa atcatttttt
     6421 cccgagccta gaaataaaag agcgaggctg attttgtaaa catttgttta ctaaaaaatt
     6481 taaagtgggc gtttatttgg cataccagcc ttacgtggat atttatttgg tgtttctttt
     6541 ttcttttcta ccactgtgat atagcgcgga tctctattcg gtagggcgta gctgagattg
     6601 tcttcgacct tactaaaaag gagattgagg gcattcttag cttctaataa ttcctcaggc
     6661 gcattgctgg ccttgagtgc caatagtttt ccaccaacct taaggtaggg aatggtcaat
     6721 tcagatagga cctgcatacg ggcaaccgca cgagctgtta caaaatcata ttgagcacgg
     6781 aagttcttgt cttgggcaaa atcttcggca cgtccgtggt agaaatgaac tccgttcaaa
     6841 tccagttctt gagccaagag ttgtaggaag ttgatgcgct tattgagtga atcaataatg
     6901 gtcacatcta actccggata gagaattttc attggtagac taggaaatcc tgccccagcc
     6961 ccgatatcaa gaagtttgat agtttcattg ggaatcaaac cttgaagaat gggtgcaatc
     7021 gaatcgtaaa aatgtttgag ataaacttct tccttgtccg taatcgccgt caaattaatc
     7081 ttctcattcc actcgaccaa gagctcaaaa taacgttcaa attgttcttt ttgctggtcc
     7141 gaaagtggaa gattctgctc ggcaagcaag ttgtaaaatg tttttggttt catagttatt
     7201 tccttcttta gtatcatctt attttaccac aatcatagaa aatttgatat actggtacta
     7261 atctaaaagc agaaaggact tatattatga cttggattat tcttggagtt atcgctctta
     7321 ttgttatttt tgtgattgtt agctataacg gtttggttaa aaatcgtatg caaaccaagg
     7381 aggcttggag tcagattgat gttcagttga aacgtcgaaa tgacctcttg ccgaacttga
     7441 ttgagactgt aaaaggttat gccaaatatg aaggttctac ccttgaaaag gtggcagaac
     7501 tacgtaacca agtggcggca gcgacttcac cagcagaagc tatgaaagcc agtgatgccc
     7561 tcactcgtca ggtttcaggt atttttgcag ttgcagaaag ctatccagat ttgaaagcta
     7621 gtgctaactt tgttaaattg caagaggagt tgacaaacac agaaaataaa atttcttact
     7681 ctcgtcaact ctataacagt gttgtcagca actacaatgt aaaattagaa actttcccga
     7741 gcaatattat cgctggaatg tttggattta aagcggcaga tttccttcaa acacctgaag
     7801 aggaaaagtc ggttcctaaa gttgatttta gcggtttagg tgactaagat gttgtttgat
     7861 caaattgcaa gcaataaacg aaaaacctgg attttgttgc tggtattttt cctactctta
     7921 gctcttgttg gttatgcggt tggttatctc tttataagat ctggacttgg tggtttggtt
     7981 atcgcactga ttatcggctt tatctacgct ttgtctatga tttttcaatc gacagagatt
     8041 gtcatgtcca tgaatggagc gcgtgaggtg gatgagcaaa cggcaccaga cctctaccat
     8101 gtagtggaag atatggctct ggtcgcccag attcctatgc cccgtgtttt catcattgat
     8161 gatccagcct taaatgcctt tgcgacaggt tctaatcctc aaaatgcggc tgttgctgcg
     8221 acttcaggtc tactagctat catgaatcgt gaagaactag aagctgttat gggacatgaa
     8281 gtcagtcata ttcgtaatta tgatatccgt atttcgacta ttgcagttgc ccttgctagt
     8341 gctatcacca tgctttctag tatggcaggt cgtatgatgt ggtggggtgg agcaggtcgc
     8401 agacgaagtg atgatgaccg agatggaaat ggtcttgaaa tcattatgct agtggtttcc
     8461 ctactagcta ttgtactggc acctctcgct gcaaccttgg ttcagctcgc tatttctcgt
     8521 cagagggaat ttctggcaga tgcatctagt gtcgagctga ctcgcaatcc ccagggaatg
     8581 attaatgccc tagataagtt ggacaatagc aaacctatga gtcgccacgt cgatgatgct
     8641 agcagtgctc tttatatcaa tgatcccaag aaaggtgggg ggttccaaaa actcttttat
     8701 acccacccac ctatctcaga acggattgaa cgtttaaaac agatgtaaaa tgaaggctgg
     8761 aaaaaagtct ttaaaatctg aaaaacgcat aatatcaggt gtgaaaactt gatattatgc
     8821 gttttactat gggaagattt acttcttttt ctcctaaaat tgtgtttttg ccccacctat
     8881 ctgctatgtt gcaaatttat aaatcttcta aattaactag cttcaaaccg tgttctgtca
     8941 tgcgatagat gatcgagcag atttctttta agaaacgacg gtcatgcgaa acagtgatga
     9001 gaccgcctgg ataggttgca aagagttttc tgatttgggg ttgagaagtg ggagaaaagt
     9061 ttcgtgtggg ttcatccagc aggagaaagt ttggtttgcg caggactaaa tccaaaagca
     9121 ggagttttcc ctgttgtccg ccagataagg agcgaatttg atgctgcatt tctggataac
     9181 tgaaattgag actggctagg tgagattgga ttttctgaag ttcctctttt tccccagttt
     9241 tgctgatata agctattggt gataaatcca attgcagttt tttgtggtaa tcttgtggca
     9301 taaaaccaag ggaaatctct cttttatcat tcagaagttg ctttaatttg gctaacagag
     9361 ttgatttccc aacaccattt ggcccgataa taccgatttt ttcttgccca cggacagtta
     9421 gttgtagttt ttgaaccaaa actcggtcgt caatggacaa attttctttt tccagttgga
     9481 ctaagacttt agaagctggt aatggttgga tgtctgaaaa gaaaagttgg atttgttcct
     9541 cttcaagtgg cttttgagtc atggactgag ctgtcttttc gtagcgtttt tcttgtgaga
     9601 ggacagtttt catcttttta gccaataggc gaccggcagt actatctttg gtagctcgaa
     9661 gcgcagtttc tacattttgc ttaactctcc gatgtttttc catggtttta tcgtaggctc
     9721 tttggttgtt agcagcttgc tgactttgtt tggcaaaatt agcctttctc tgctcactat
     9781 agctatcata gtctaaatgc tctactagcg tttccgcttc tttacggtgt ttgaccagtc
     9841 gcaagtgaac aatagtgtct gctgtttcag aaagaaagtc ttcatcatgg gaaatgaaaa
     9901 taacggtttg cctggtcttt tgaatctggc cttttagcca atcaactgtc tcaaggtcta
     9961 ggtcatttga aggttcatct aaaaatagaa tctcaaaggg tttggctaac tcatggataa
    10021 gctgaatttt caaagcttct ccccctgata gattgccaat cttttggtca cttgcgaaac
    10081 gattgctatc aaaatgcaat tcctccgcca aacgatagag gatactgtag tctaaatcaa
    10141 tagaatctaa aaagaagtag tcgtgtaaag ttttcttttt cagctcctcg ggaagttttt
    10201 gaggaatgta ggccagtgac tgatagtcag attggatgtt tcccttgata gtgaaatcag
    10261 acaaagcttc ccccattaaa attttaagta aggttgattt gccatttcct tcttcaccaa
    10321 taatagcaac cttttccccg tcttggatgg tcatgcttag gtcagataca agatctcgta
    10381 aatctttgtt ttgtgtgatg gttagatgat tgatttttat catatgattt ccctttctag
    10441 aatgttcata gtgcataaca aaaagctcta ctttacctag tagagcccaa agtctatgtc
    10501 aaaaaaactc tattaacatg ccagaaaaag agcataaaag agcacacaaa aagagacaaa
    10561 aacaagatct actaaataaa ggttctttat ttgatagact tagttgttca tgtctccctt
    10621 acctccgagt attagtatct ttatattata cctttaagga aattttgtca acagaaaact
    10681 ggaaattcta tgctctaaaa tccaaaaaca ggtccataga gacttagtac gtgtttgaca
    10741 tgctcgtaat ggaatttatc atagtttcgc caagcagtgc gtgcttgatc gtttagtttt
    10801 aggctaccca gtccaatcag aagactggtg ggttggtaaa atttctcaag atcaattaag
    10861 atactgttgt tcaacttttc cgtttttttg agctcttgca tagtagtgtt tagcagctct
    10921 tgtagtcgaa tatcatctgc cgtaccctgt ttacagtttt ggtagctttt atttaacttg
    10981 gataggagtt gaatgagttt cttgtcttcc atgtgagcat cctccttgat ctgatgtact
    11041 ctttccctat agtatagcac taaaaaacaa aactgtcata gtaaatatgt taaaattctg
    11101 aattttaatt acaaagtttg gtgtgctgat ttgtagcctt ttcatggtat aatcaagaga
    11161 gtaaatcttt atggaggaag tatgaaatta aatattcaag aaattcgtaa gcagtctgaa
    11221 ggtttgaact ttgaacaaac gttagattta gttgatgacc tgcgtgcacg taatcaagaa
    11281 attttagatg taaaagatat ccttgcagtt gggaaagtac aatatgaaga ccgtatgtat
    11341 ttcttagatt atcaactatc ttataccatt gttcttgctt cgagtcgcag tatggagcca
    11401 gttgagttag ttgaatctta tccagtcacg gaagttttca tggaaggcgc aactaaccat
    11461 ctaaatcaag aaattttaga tgatgacttg gtcttgccca tcgaaaatga ggcgcttgac
    11521 cttgctgaga gtgtatcaga caatatcttg ctaaacattc ctatcaaggt cttgacggct
    11581 gaagaagaag ctggtcaagg atttgtctca ggaaatgact ggcaaatcat gacagaggaa
    11641 gaataccaag ctcaacaagc agtaaagaaa gaagaaaaca gtccttttgc tggcttacaa
    11701 ggactatttg acggagatga ataatggtct tgtcaaaaaa acgagcacga aaggtgctag
    11761 aagaaatcat tgccctcttc ccagatgcca agcctagtct tgattttacc aatcattttg
    11821 aactcctggt tgcggtcatg ttatcagccc agacgacaga tgcagcggta aataaggcca
    11881 caccaggtct ctttgttgcc tttccaacac cccaagctat gtctgtagcg acagagagtg
    11941 agattgcttc acatatttct cgcttgggat tgtatcggaa taaagctaaa ttccttaaaa
    12001 aatgtgccca acagttacta gacgattttg atggtcaagt ccctcagaca cgtgaagaat
    12061 tggagagttt ggcaggtgtt ggtcgcaaga cagccaatgt tgtcatgagt gtaggatttg
    12121 ggattccagc ctttgcagtt gatactcatg tggagcgtat ttgcaaacac cacgatattg
    12181 tcaaaaaatc agcgacgcca cttgaggtgg aaaagcgggt catggatatc ttgccgcctg
    12241 agcagtggtt agctgcccat caggccatga tttattttgg aagagccatt tgccatccaa
    12301 aaaatccaga gtgtgaccag tacccacaat tatatgattt tagcaatttg taagatgcta
    12361 cattgaaact ttttgcttga ttttaagcat gctttgtgct atagtaaatg ataactaaat
    12421 attcctttaa acctgtcccg tgaggcaggc aaggagcgcg ataaagtaga agggtgaagt
    12481 ctatactgtg tgcagtaggg ctcgcttggc tataaatttc aagtctcctt gtttaaggag
    12541 acttttcttt ttggaggttt gtcatgaaaa caaaagaagt tgtagacgaa ttgaccgtca
    12601 aacgagcgat tacgcgtatt acttatgaga ttatcgaacg caacaaagat ttgaataaga
    12661 tcgtcttggc tggtattaaa actcgtggtg tctttattgc ccaccgaatc caagaacgtt
    12721 tgaagcagtt agaaaatctt tcagttcctg ttgtggaatt ggatactaaa cctttccgtg
    12781 atgatgttaa aagtggagaa gatacttctt tggtttctgt cgatgtgaca gaccgcgaag
    12841 ttatcttggt ggatgatgtg ctctatacag gtcgtaccat ccgtgctgct attgataata
    12901 ttgtcggtca tggtcgtcct gcgcgtgtga gtttagcagt tctagtcgat cgtggacata
    12961 gagaattgcc aatccgtcca gattacgttg gaaaaaatat cccaaccagt cgttctgaag
    13021 aaatcatcgt agagatgaca gaacttgatg accaagacag agttctgatt actgaagaag
    13081 cttagaaagt taaaggagta gccatgtcag aaaatcaaca agcattgaac catgtggtgt
    13141 ccatggaaga cctcactgtc gatcaagtaa tgaaattgat caagcgagga attgagttta
    13201 aaaatggagc ccagcttccc tatgaagacc atccgattgt ttccaatctc ttctttgagg
    13261 attctacacg gacacataag tcctttgaag tcgcagagat taaacttgga ttggaacgac
    13321 ttgactttga tgtgaagact agttcggtta ataagggtga gacactttat gacaccattt
    13381 tgactctgtc tgctttagga gtggatgtct gtgtgattcg ccatccagag gtcgactact
    13441 atagagaatt gattgctagt ccaacgatta cgacttccat catcaatggt ggagatggtt
    13501 cgggccaaca ccctagccag agcttgcttg atttgatgac catttatgag gaatttggcc
    13561 actttgaggg tcttaaagtt gctattgcag gtgacttgga ccactcacgc gttgccaaat
    13621 ccaatatgca gattttgaaa cgcttgggat ctgaactctt tttcgctgga cctgaggaat
    13681 ggagaagtca agagtttgca gactatggac agtttgtaac cattgatgaa atcattgatc
    13741 aggttgatgt catgatgttt ctccgtgtgc aacacgaacg ccatgatagt ggagctgtat
    13801 tttcaaaaga agactaccat gcccaacatg gcttgactca agaacgttac gatcgtttga
    13861 aagaaacagc aatcctcatg cacccagctc caatcaatcg tgatgtagaa atagcagacc
    13921 acttggttga agcaccaaaa tcacggattg tccaacaaat gaccaatggt gtctttgttc
    13981 gaatggcaat cttagaatcc gtactagcga gtagaaacgc caactaaaac acaagacgtt
    14041 aaaagacgcc agtgagcgtc cacgagaggt gaaaatatga caaaaagaat tctagtatta
    14101 gaagatggca cagtttttga aggcaaggcc ttcggagcag atattgatgt aacaggcgaa
    14161 atcgtcttta atacagggat gaccggctac caagaatcca ttacagacca gtcttataat
    14221 ggacaaatct tgacctttac ttatcctttg gtaggaaatt atggtattaa tcgtgatgat
    14281 tatgaatcca ttattccaac ttgtaaggga gtcgttgttt ttgaagaagc gcgtagagct
    14341 agcaactggc gcaaccaaat gaccttggat gaatttttga aagccaagaa aattccaggg
    14401 atttcaggaa ttgatacgcg tgctcttacc aagattatcc gtaagcatgg tactatgcgt
    14461 gcaaccttga cccatgttgg ggacagtatg gaccatgtga cggaccagct ccaagcaaca
    14521 gttttaccga cagacaatat caaacaggtt tctactaaaa cgtcttatcc agctccagga
    14581 gttggtttga gcgtggtgct agtggacttt ggtctcaagc actcaatcct acgtgaactt
    14641 tctaaacgca actgtaacgt gacagttgtt ccttattcga caacggctga agaaattctc
    14701 catctcaatc ctgacggagt tatgttgtca aatggtccag gtaacccaga agacgttcca
    14761 caagcactcg acatgattcg cggtgtacaa ggaaaaattc caatctttgg tatttgtatg
    14821 ggacaccaac tctttgcaat ggcaaacggg gctaagacct ataagatgaa gtttggccac
    14881 cgtggattca accatgcggt acgtgaaatc gcaacaggac gagtagactt caccagccag
    14941 aaccatggtt atgcagtcag ccgcgaggat ttgccagagc acttgattat tacccacgaa
    15001 gaaatcaatg acaagtcagt ggaaggtgtg cgccacagat accaacctgc tttctctgtt
    15061 caataccacc cagatgcagc tcctgggcca cacgacgcta gctacctatt tgacgagttt
    15121 atcgagatga tggaagtttt taaacaatca aactaagaac gagtaaaagc tcgtcggaga
    15181 atttatgtaa ctgaacacgg acggaaagct cagaatgtag agaaaccaac ttggattgtc
    15241 aatccttcct tgatttccta aaattcaatc gctttctatt cgtcctttgt atcttattta
    15301 atgtcgccct gagaggcgac gaatagaaag gagaagggag ctccgtattt gctgaactga
    15361 atacgggcta cggacggtgc taaaaagata gttattccta gaactgacag ttcttcgtca
    15421 taactcctat tttagctttg tccgcttgac acccttaata tcttatatat gcctaaacgt
    15481 actgatattc aaaaaattat ggtgattggt tctggtccga ttattattgg tcaggctgct
    15541 gagtttgact acgctgggac tcaggcttgc ttgtcgttga aagaggaagg ttatgaggtt
    15601 gttttggtta actcaaatcc tgcaaccatc atgacggaca aggaaattgc tgacaaggtt
    15661 tacattgaac cgattacact tgagtttgtg acacgtattc ttcgtaagga aggtccagat
    15721 gccttgctac caacactcgg tggtcagaca ggtctcaaca tggccatgga attatctaaa
    15781 aatggtatcc tagacgagct tggcgttgaa cttctgggta ccaaattatc tgccattgac
    15841 caagcggagg accgtgatct ctttaaacaa ttgatggaag agcttgaaca acccattcca
    15901 gaatctgaaa ttgttaacac agtagaagaa gcagttgcct ttgcagcgac aattggctac
    15961 ccagtcatcg ttcgtccagc ctttaccctt ggtggtactg gtggtggtat gtgtgccaac
    16021 gagaaagaat tgcgtgaaat cactgaaaat ggtttgaaat tgtcacctgt tacccaatgt
    16081 ttgattgagc gttcgattgc cggtttcaag gaaattgaat acgaagtgat gcgtgactca
    16141 gctgacaatg ccttggttgt ttgtaacatg gaaaactttg acccagttgg gattcacaca
    16201 ggggattcca ttgtatttgc ccctgcgcaa accatgtcag actatgaaaa ccaaatgctt
    16261 cgtgatgcga gcttgagcat tattcgtgcc ctcaagattg aaggtggatg taatgttcag
    16321 ctagctcttg atcctaacag cttcaagtat tatgttatcg aagtaaaccc tcgtgtatca
    16381 cgttcgtcag cccttgcttc taaagcgaca ggatatccga ttgctaagtt ggctgccaag
    16441 attgcagtag gtttgacctt ggatgaggtt ataaacccag ttacaggttc aacctatgcc
    16501 atgtttgaac cagcccttga ctacgtagtt gctaagattc cacgtttccc atttgacaag
    16561 tttgaaaagg gtgaacgccg tctcggtaca caaatgaagg cgactggaga agtcatggca
    16621 atcggtcgta acatcgagga atctcttctc aaggcctgcc gctcccttga aattggggtg
    16681 caccacaatg aaatacctga acttgcagca gtttctgatg ataccttgat tgaaaaggtt
    16741 gtcaaagccc aagatgaccg tctcttctac gtatcagaag ccattcgtcg tggctacaca
    16801 cctgaagaaa ttgcagagct tacaaaaatt gatatcttct atcttgataa actcttgcat
    16861 atctttgaaa ttgagcaaga attgggtgct catccacaag atctagaagt tttgaaaaca
    16921 gccaaactta atggtttttc agaccgtaag attgctgaac tctggggaac gactgatgat
    16981 aaagttcgcc aacttcgctt ggaaaacaag attgttccag tttataagat ggtagacact
    17041 tgtgcggcag agttcgattc cgaaacgcca tatttctatt caacctatgg ttgggaaaat
    17101 gaatctatca aatctgataa agaatctgta cttgtcctag gttcaggtcc aatccgtatc
    17161 ggtcaagggg ttgagtttga ctacgcaacc gttcactctg ttaaggctat ccaggcagct
    17221 ggttatgaag ctatcatcat gaactcaaac ccagagaccg tttcaacaga cttctctgta
    17281 tcagataagc tttactttga gccattgaca ttcgaagatg ttatgaacgt cattgacttg
    17341 gaacaaccaa agggagttat cgttcagttc ggtggtcaaa cagccatcaa ccttgcagag
    17401 ccattggcaa aagcaggtgt gaccatcctt ggtacacagg ttgctgacct agaccgtgcc
    17461 gaagaccgtg acctcttcga gcaagctctc aaagaattgg atattccaca gccaccagga
    17521 caaacggcta ccaatgaaga agaagcagct cttgcagctc gtaagattgg cttcccagtt
    17581 cttgtccgcc catcttatgt acttggtgga cgtgccatgg aaatcgttga aaacgaagaa
    17641 gaccttcgtt cttacatgcg aactgctgtt aaggctagtc cagaccatcc agttcttgtc
    17701 gactcttata tcgttgggca agagtgcgaa gttgatgcca tttcagatgg taaaaatgta
    17761 ctcattcctg gtatcatgga gcatatcgaa cgtgctggtg tccactcagg tgactcaatg
    17821 gctgtttacc caccacaaac cttgtcgcaa aaggttcagg agaccatcgc agactacacc
    17881 aaacgcttag caatcggtct tcactgcctt ggaatgatga acatccagtt tgttatcaag
    17941 gatgaaaaag tctacgttat tgaggtcaat ccacgtgcca gccgtaccgt tccattccta
    18001 tctaaggtaa ccaatattcc tatggctcaa gtagcgacca agctcattct tggtcaaagt
    18061 ttatcagaac ttggctacca aaatggactt taccctgaaa gcacccgtgt tcatatcaag
    18121 gcacctgtct tctcctttac caaactagct aaggtagaca gcttgctcgg tcctgaaatg
    18181 aagtcaacag gtgaagttat gggttctgat gctactttgg aaaaagctct ctataaagcc
    18241 tttgaagctt cttatctaca cttgccaact tttggtaatg tcgtctttac catcgctgat
    18301 gatgccaaag aagaagcctt gaacttggct cgtcgtttcc aaaacattgg ttatggaatc
    18361 cttgcgacag aagggacagc agccttcttt gctagtcatg gcttgcaagc ccaacctgtt
    18421 ggtaagattg gtgatgacga taaggatatc ccaagttttg tacgcaaagg aagaattcaa
    18481 gctatcatta acacagtagg aacaaaacga actgctgacg aagatggtga gcaaattcgt
    18541 cgttcagcca ttgaacacgg ggtgcccctc ttcacagcgc tagatacagc taatgccatg
    18601 cttaaggtac ttgaaagtcg tagctttgtt acagaagcga tttaaataga aaaataagat
    18661 atagcagact gaa
//
LOCUS       DAVKMU010000037        18197 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKMU010000037 DAVKMU010000000
VERSION     DAVKMU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.037"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(85..1020)
                     /locus_tag="VW343_001514"
     CDS             complement(85..1020)
                     /locus_tag="VW343_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9756047.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1017..1877)
                     /locus_tag="VW343_001515"
     CDS             complement(1017..1877)
                     /locus_tag="VW343_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9756048.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1985..4147
                     /locus_tag="VW343_001516"
     CDS             1985..4147
                     /locus_tag="VW343_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9756049.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4239..5498
                     /locus_tag="VW343_001517"
     CDS             4239..5498
                     /locus_tag="VW343_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9756050.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5512..6378
                     /locus_tag="VW343_001518"
     CDS             5512..6378
                     /locus_tag="VW343_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9756051.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTRWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6392..7225
                     /locus_tag="VW343_001519"
     CDS             6392..7225
                     /locus_tag="VW343_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9756052.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKLTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7226..7306
                     /locus_tag="VW343_001520"
                     /pseudo
     CDS             7226..7306
                     /locus_tag="VW343_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7583..9025
                     /gene="gtfA"
                     /locus_tag="VW343_001521"
     CDS             7583..9025
                     /gene="gtfA"
                     /locus_tag="VW343_001521"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9756053.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTIEIETPTVHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9163..10356
                     /locus_tag="VW343_001522"
     CDS             9163..10356
                     /locus_tag="VW343_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9756054.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11043..13022
                     /locus_tag="VW343_001523"
     CDS             11043..13022
                     /locus_tag="VW343_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9756055.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFARPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13089..14585
                     /locus_tag="VW343_001524"
     CDS             13089..14585
                     /locus_tag="VW343_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756056.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14585..15511
                     /locus_tag="VW343_001525"
     CDS             14585..15511
                     /locus_tag="VW343_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756057.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15520..16587
                     /locus_tag="VW343_001526"
     CDS             15520..16587
                     /locus_tag="VW343_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756058.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16598..17524
                     /locus_tag="VW343_001527"
     CDS             16598..17524
                     /locus_tag="VW343_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756059.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQTELARYQKGLN"
     gene            complement(17599..>18197)
                     /locus_tag="VW343_001528"
     CDS             complement(17599..>18197)
                     /locus_tag="VW343_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9756060.1"
                     /translation="TVSLPNAKIVLDRFHIVQHLSRAMSRVRVQIMNQFHRKSHEHKA
                     IKHYWKLIQQDSRKLSDKQFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLYQLLLFH
                     FQNKEPEKFFELIEDNLKQVHPIFQTVFKTFLKDKEKIINALQLHYSNAKLEATNNLI
                     KLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         5353 a         3227 c         3830 g         5787 t
ORIGIN      
        1 caataaaact tcagatccgt aaaacttaat tgaattacta atctgaagtt atttttatat
       61 cgtttatatt ataattgtgt tattttactt ccaactattg agagaaattt cgccactatt
      121 tagccagatt tcttttccgt tatcacattg aactaaaagt tttccatttt ctgagatgtc
      181 tttagcaagt cccttgtagt ctttttgctc tagtgtgaaa gtgacttctt ttcctagaat
      241 gaatgactgt tttttgtata ggtataatag ctcttctgct ggtgtttcga agaaagcacg
      301 ccagatttct atgatcaatt cattccttgt tataggagct gtagctttaa ataagctggc
      361 agctttttct tttaattcct gagggaagtc tttaatagtg aaattgatac ctactccaat
      421 aatgatatct gtgactaagc cagtttctac agaggtcatt gcttcagtaa ggattcctcc
      481 aattttatga ttgtttagat agatatcatt gacccatttt atgtcgacat ctattaaagt
      541 taggttctta atggctttgt agacagctcc agctacaagt agtgtgtagg atggtaattt
      601 gtcatagggg agatttggtt taagatggag tgtcatataa ataccacctt gtggtgagta
      661 gaaggaacgt tgaaaacggc ctcggcctgc tgtttgatag gaagctagat agagggtatt
      721 tgcttcatgg cctaaatcaa ttgcttcttt tgcatctagt tgtgttgatt ttgtttcggg
      781 tttaaagctg actttaattg gaagattttc ttctagaatc tctggaagaa taaggtcacc
      841 attcatcagt ttatatcctc tatttttgat actatcaatt tcaatgcctt cttgttctag
      901 tcgcttgatg gctttccaaa ttgctgttcg gcttagggat agtttttctg cgattttttc
      961 tccgctgata tagtcggttt ctttagatag gatttggtag acagcttggt aggatttcat
     1021 aatgttgcct ttcttactag ttggtattga gagtattctt ttcttgtatg acttggagtc
     1081 tgattaaagt attgtttata agctttcgaa aaatggagtg gatctgaaaa acctaccgag
     1141 tatgcaatta ccttgatgga ctcttgggta ttttcgagaa gttgtctagc tcggtgcatt
     1201 cgaacgtaga gtaggtattc tttgggtgat aaggtattaa attctttgaa tacgcttgat
     1261 aagtagcttc tgtgaacgga tagttctttt gctaaatctt gaattgtaag tgattgagga
     1321 tagtggctat caattaatcg tttgcattca agatagagtt ggtgggttga tgaaatattc
     1381 ttttttttct gattgggagc aatagttccc agatgaaaca tcagttcatg aagttgtccc
     1441 atgatatgga gttgagctaa ttcacttgat tttgtaatct gagcgaagcg gacaatgtct
     1501 gagatgagtt ttgcagtagt ctgggtatga caagtttcag attggatgag ataggattga
     1561 tcagaaattt gggaaagagc aaaataatca ggggctttcc ctccagtgat tcctaaccag
     1621 tagtaggccc aaggttcttt actatctgct tgataaaagg ttagttcctc tggttttaat
     1681 agaaagaaat ctccttcttt taaatcaaca attttaccct tgtaatgaaa ttttccttgt
     1741 cctttagtaa tgtaatgtag gacgtatgta tcacgaatgg ctggaccaaa agagtaatta
     1801 ggtgtgcatt cctcatatcc ataaaagctt agggcaaggt cgattgttcc agtctggtat
     1861 tctgaaaaaa ctaacatgtg ctacctccta cctaacattt taccatattc ttgagacatt
     1921 tttctatttt ggaaagcgat ttcaggtgat aaaatagagt caataaagtg atgaggtgaa
     1981 gtaaatggga gttaggatag agaataatct attttatgtt gagagtaaaa atctaagttt
     2041 gattattgaa aatcgaaatg gctacttact tttgaaacat ttaggaaaga ctattaagaa
     2101 ctataaaggt tccaatagtg tttatgaacg agaccatgcc ttttcaggaa atccaacagc
     2161 tactaatcga acctttagtt tagatactca acgtcaaatt tttggacaac atggcttagg
     2221 agattttagg aaaccaacca tacaggttca gcataatgta actgaagtaa cagactttcg
     2281 atttgtagaa gcaaagattt taaaaggtca gaatggtcca cagggcttac cttctccaca
     2341 tagcatggac gatacagaga ctcttgtctt aatgttagaa gattctaagg ctcaacttag
     2401 tctgactttg tattatacta cttttaataa tgatgcgact attgctagct acagtaaatt
     2461 agataataat agtaatcagg aagttgtcat ccataaagat ttttctttta tggctgattt
     2521 tccagctgca gattacgaaa tagtaactct gcagggtgct tatgctcgtg aaaagactgt
     2581 tagacgtcaa caggtagaac aaggaatctt ttcgattagt tcaaaccgag gtgcttctgg
     2641 tcatgctcaa acaccagctc ttctactatg cgaacaagga gtcacagagg atgctgggaa
     2701 tgtgtttgct attcaactaa tgtatagtgg caactttgaa gcttttgttc aaaaaaatca
     2761 attgaatgaa gttcgggtgg ctattggcat taatccagaa aacttttctt ggaagttagc
     2821 ccctgaggaa tactttgaaa caccggtagc tttagtgact cattcagatc agggattaac
     2881 tggtattagt catgaaagtc agaattttgt actgaagcac attatgctaa gtgaattttc
     2941 taaaaaagaa cgtccaattc taatcaataa ctgggaagct acttactttg actttcagag
     3001 agaaaaactg ttagagttag cagatgaagc taagaaagtt ggcattgaac tttttgtatt
     3061 agatgatggt tggtttggca atcgttttga tgataatcgt gctttaggtg attgggttgt
     3121 taatgaggaa aaactgggtg gaagtctaga aagtctgatt tcagctatcc atgaaagagg
     3181 tttgcagttt ggactttggt tagaacccga aatgatttct gtagatagtg atttgtatcg
     3241 tcaacatcct gactgggcta ttcaggttcc tggctatgag catacttatt ctcggaatca
     3301 attagtactt aatcttgcca atcctcaggt agtagaatac ttgaaaagtg tcttagatca
     3361 actcctatct tatcatgaga ttgattacat taaatgggat atgaaccgca atatcactaa
     3421 gctagggaat ggattaactt atctagagac acagatgcaa tctcatcagt acatgctggg
     3481 gctttatgaa ctcgtttctt atctgacaga gaagcacagc catattctct ttgagtcctg
     3541 ctctggtggt ggtggacgaa atgatcttgg tatgatacgc tatttcccac aagtctgggc
     3601 tagtgataat actgatgcca ttgcacgttt accaattcaa tacggttcat cctatttcta
     3661 tccaaccatt tctatggggg ctcatgtgtc agcagtaccg aatcatcaga tgggacgaat
     3721 gacaccatta gaaacacgtg gccatgtagc aatgatggga aatttgggct atgagcttga
     3781 tttgacaaat ttatcagatg aagagaaagc tacgattgct aatcaggtga acttgtataa
     3841 agaattacga ccagtagttc agttaggaca gcagtataga ctaattaatc ctgatactgc
     3901 atccaatgaa gcagctgtac aatttaatta cggaaatcaa acgattgtaa cctacgttcg
     3961 cgttttatct gttgtagaga ccatggaaac aactttaaaa ttaaaagatt tggatgaaga
     4021 gggactatat aaattacagg aaaatggcga agtttactca ggtgcagaac taatgtatgc
     4081 gggcttaact gttatcttat cccaaggaga ttttttgagt agacagtata tttttagaaa
     4141 actatgatat gtactgaccc caaaagttag atttattctg tctaactttt ggggtgcagt
     4201 tcaataaggg tgtataaatt tataaaggag gctttctaat ggaatggtat aaaaaaatcg
     4261 gacttcttgc aactacaggt ttagctttgt ttgggctcgg cgcttgctcc aactatggta
     4321 aatctgcgga tggcacagtg accatcgagt atttcaacca gaaaaaagaa atgaccaaaa
     4381 ccttggaaga aatcactcgt gattttgaga aggaaaaccc taagatcaag gtcaaagtcg
     4441 tcaatgtacc aaatgctggt gaagtattga agacacgcgt tctcgcagga gatgtgcctg
     4501 atgtggtcaa tatttaccca cagtccattg aactgcaaga atgggcaaaa gcaggtgttt
     4561 ttgaagattt gagcaacaaa gactacctga aacgcgtgaa aaatggctac gctgaaaaat
     4621 atgctgtaaa cgaaaaagtt tacaacgttc cttttacagc taatgcttat ggaatttact
     4681 acaacaaaga taaattcgaa gaactgggct tgaaggttcc tgaaacctgg gatgaatttg
     4741 aacagttagt caaagatatc gttgctaaag gacaaacacc atttggaatt gcaggtgcag
     4801 atgcttggac actcaatggt tacaatcaat tagcctttgc gacagcaaca ggtggaggaa
     4861 aagaagcaaa tcaatacctt cgttattctc aaccaaatgc cattaaattg tcggatccga
     4921 ttatgaaaga tgatatcaag gtcatggaca tccttcgcat caatggctct aagcaaaaga
     4981 actgggaagg tgctggctat accgatgtta tcggagcctt cgcacgtggg gatgtcctca
     5041 tgacaccaaa tgggtcttgg gcgatcacag cgattaatga acaaaaaccg aactttaaga
     5101 ttgggacctt catgattcca ggaaaagaaa aaggacaaag cttaaccgtt ggtgcgggag
     5161 acttggcatg gtctatctca gccaccacca aacatccaaa agaagccaat gcctttgtgg
     5221 aatatatgac ccgtccagaa gtcatgcaaa aatactacga tgtggacgga tctccaacag
     5281 cgatcgaagg ggtcaaacaa gcaggagaag attcaccgct tgctggtatg accgaatatg
     5341 cctttacgga tcgtcacttg gtctggttgc aacaatactg gaccagtgaa gcagacttcc
     5401 ataccttgac catgaactat gtcttgaccg gtgataaaca aggcatggtc aatgatttga
     5461 atgccttctt taacccgatg aaagcggatg tggattagga gtagagagac tatgaaaaaa
     5521 gtattacaaa aatattgggc atgggctttt gtggtcatcc ccctcttgtt acaagcaatt
     5581 ttcttctatg tgccgatgtt tcaaggagcc ttttacagtt ttaccaactg gacaggattg
     5641 acttacaact acaaatttgt cggcttaaac aactttaagc tcctcttcat ggatccaaaa
     5701 ttcatgaatg cgattggctt taccgcaatc attacgattg ccatggtggt tggtgagatt
     5761 gcactcggga tcttcattgc gcgtgtcttg aactctaaaa tcaaaggcca aaccttcttc
     5821 cgtgcttggt tcttcttccc agctgtttta tctggtttga cagtggcttt gatcttcaag
     5881 caagtcttca actacggtct tccagcgatt gggaatgccc ttcatattga atttctccaa
     5941 accagtcttt tagggactag gtggggagca atctttgcgg ctgtctttgt ccttctttgg
     6001 caaggggtgg ctatgcccat catcatcttc ctagctggtt tgcaatctat tccaactgag
     6061 attacagagg cagcaaggat tgatggtgcg actagcaagc aagttttctg gaacattgaa
     6121 ttgccttact tgctaccaag tgtctctata gtctttatcc tagccctaaa aggtgggctg
     6181 actgcctttg accaagtctt tgccatgacc ggtggtggtc caaacaatgc cacaacctca
     6241 cttgggctct tggtttataa ctatgccttt aaaaacaacc aattcggtta tgccaatgcc
     6301 attgccgtaa tcttgttctt cttaattgta gtgatttcga tcatccaatt gagagtatct
     6361 aagaaatttg aaatttaaga ggagaagcat gatgaaacaa gatgaaagaa aagccctgat
     6421 tggcaaatac attctattga ttctaggatc ggttctgatt ttagtgccgc tccttgctac
     6481 cctctttagt tccttcaaac tcactaagga tattgtagat aatttctttg gctttccaac
     6541 caacttcaca tgggacaact ttagccgtct cttagctgat gggattggag gctattattg
     6601 gaactctgtc gtcatcactg tcttgtcttt acttgcagta atgatcttta tccctatggc
     6661 agcctactcc atcgctcgca atatgagtaa aagaaaagcc tttaccatca tgtataccct
     6721 cttaatcctc ggaatcttcg tacctttcca agtcatcatg attccgatta cggttatgat
     6781 gagtaaactc ggtttggcta atacctttgg tttgatcttg ctctacttga cctatgcgat
     6841 tccacagacc ctctttctct atgttggcta tatcaaaatc tcgattccag aaagtctgga
     6901 tgaagcagca gagatcgatg gggctaatca atttacaacc tatttccgca tcatcttccc
     6961 aatgatgaaa ccgatgcatg cgacaaccat gatcatcaat gccctttggt tctggaatga
     7021 cttcatgttg ccactccttg tcttgaaccg ggattccaaa atgtggactc tgcctttgtt
     7081 ccaatacaac tacgcaggcc aatatttcaa cgactacgga ccaagctttg cctcttacgt
     7141 ggtcggcatt atcagtatca ccattgtcta tctcttcttc caacgccata tcatttcagg
     7201 aatgagcaac ggggcagtga agtaatactc aatgaaaatc aaagagcaaa ctaggaagct
     7261 agccgcaggt tgctcaaaac actgttttga ggttgtggat gaaactgacg aagtcagtaa
     7321 ccatacctac ggtaaggtga agctgacgtg gtttgaagag attttcgaat agtataagaa
     7381 gagctcatgg aatctatagt ctttgacaca gaggaagagt tcagcttgtt gataattcta
     7441 tgtagaaaac aagaaagtag tactttgttt atcacagtct atattagtac gagtttgatg
     7501 ataaatagag atttaaaatg aattttattt atgaagccag atcatatgag acttggcttc
     7561 aattagaaaa aggagagtaa tgatgccaat tcaaaataaa accatgttga ttacctattc
     7621 tgatagcctt ggaaataatc ttaaagactt atatgataat ttggaagagc attttggaga
     7681 tgctattgga ggagttcacc ttttaccatt tttcccatca acaggtgatc gtggatttgc
     7741 gccagttgac tacgacgaag tggactcagc ttttggtgat tgggaggatg ttaagcgttt
     7801 aggtgagaga tattatctta tgtttgattt tatgattaat catatttctc gtcaatccaa
     7861 gtattataag gactatcaag aaaaacatga agccagtgaa tttaaagatc tctttttaaa
     7921 ctgggataag ttttggccag aaaaccgtcc gacacagtct gatgtagatt taatttacaa
     7981 gcgtaaggat cgtgcaccaa agcaagagat tgtgtttgaa gatggttcag tggaacattt
     8041 gtggaatacc tttggtgagg agcagattga tcttgatgtg accaaagaag taactatgga
     8101 atttatccgt aagaccattc agcacttggc aagtaatggg tgtgatttga ttcgtctaga
     8161 tgcctttgct tatgcagtga agaaattgga tactaatgat ttctttgtgg aaccagatat
     8221 ttgggattta ttggacaaag ttcgagatat cgctgctgag tatgggacag agcttttacc
     8281 tgagattcat gaacactatt cgattcagtt taaaatagca gaccatgatt actatgttta
     8341 tgattttgct cttccaatgg tgacacttta tactctttac agttccagaa cagagcgttt
     8401 ggctaagtgg ttaaagatga gcccgatgaa gcaatttacg acgctagata cccatgatgg
     8461 gattggagta gtagatgtca aggatatcct gaccgatgag gagattgact atgcttcaaa
     8521 tgaactctat aaggttggag ccaatgtcaa acgtaagtac tctagtgccg agtataacaa
     8581 cttagatatc taccaaatca attcaaccta ctattcagcg cttggagatg atgatgtcaa
     8641 gtattttctc gctcgtctaa ttcaagcttt tgccccaggt attcctcagg tttactatgt
     8701 gggtctatta gcaggcaaga atgacttgaa attattagaa gaaactaaag aaggtcgaaa
     8761 tattaatcgt cattactata gcaacgagga aatagcaaaa gaagtgcaac gatctgttgt
     8821 gaaggccctt ctcaatctat tttctttccg taaccgttca gaagcctttg atctagaagg
     8881 gactattgag atagagacac caacagttca cagcattgta atcaaacgtc aaaataaaga
     8941 taagtccgta acagcagtag cagaaattga tttgcaaaat cagacttatc gggtaattga
     9001 gaatggagtt gaagtaacat tttgaagcct tgatatggtg gataaaatag ggtttttatt
     9061 ttggaaaacg tttcctttgt tttcaaattg ctaaaaaagt ggtacaatat aggatagctt
     9121 actattatct gaatcagctg atttggagag aaaggattca ttttgaaatc aataggcttt
     9181 attgaaaagc tgaaggggtt gtctagtaaa gagctgattt tattgggaat tatcctaagt
     9241 atctttttac ccttttatct ttttgtagtt gtactctgtt tatatattat cagtttgatt
     9301 tttacaggag acatgaaaag tattcttcag aaaatggggg agcatccgat gctgcttctt
     9361 tttcttagct atagtactgt tatatccatt cttgcacaaa attggatggg tcttgtggct
     9421 tcagtaggaa tgtttctatt tactattttc tttttgcact atcagtcgat tttatcccat
     9481 aaattctttc gattgatttt gcagttcgtc ttgtttggta gtgtcttgtc agctgctttt
     9541 gccagtttag aacatttcca aattgtgaag aaatttaact atgcttttct ttcacccaat
     9601 atgcaggtgt ggcatcagaa ccgggcagaa gtgactttct ttaatcctaa ttattatgga
     9661 attatttgtt gtttctgtat tatgattgct ttctatctgt ttacaacgac caagttgaat
     9721 tggttgaaag tattctgtgt gattgcaggc tttgttaatc tctttggttt gaactttact
     9781 caaaatcgaa ctgcctttcc tgctattatc gctggagcaa ttatctatct ctttacgact
     9841 attaaaaact ggaaggcctt ttggcttagt attggggtct tcgcgattgg tttgagtttc
     9901 ctcttttcta gtgatttggg agttcgaatg ggtactttag actcttctat ggaagaacgc
     9961 atttctatct gggatgctgg gatggccttg tttaagcaaa atcctttttg gggtgaaggg
    10021 ccattgacct atatgcactc ttatcctcgg atacatgctc cttatcatga acatgcccac
    10081 agtctttata ttgatacgat tctgagttac ggaattgtgg gtaccatttt attagttttg
    10141 tcttctgttg ctcctgttcg cttgatgatg gatatgagtc aggagtcggg gaaacgtccg
    10201 attatcggcc tttatctatc tttccttaca gtggttgctg tgcacggaat ttttgacttg
    10261 gctctcttct ggattcagtc aggctttatt ttcttgctag ttatgtgcag tattccattg
    10321 gagcatcgaa tgttggtatc ggacatgacg gattaagttt tataagtttg aaatcttcta
    10381 ccttgggtag gagatttttt tatacgatgg gcatgtcgtg catctgaggt gtaagtcctc
    10441 tgtagggatc cactaccggt gaatacaata gcgctcccca agcctgacta tcgtgaggta
    10501 gcggattaaa atggtctggg gatagatcgt tttaagtctg acgcaggaaa taagaattgt
    10561 cagagaaagc aatagtgaaa tcgtaggcta tgaatagaaa cgtgatagcg aggagcatat
    10621 agtggataag gtagcgagaa attttaaagt ataaaagata gtcttaatct atatatgtaa
    10681 atcacaaaag taattgaatt cggaataagg ttggtgtgaa aagacatagt aaactgaatc
    10741 ttgaatacta gaatgatact tctacaatat tgttataatt taatttgaat tccaaaatca
    10801 cattgttccg tccgtatttc attttactgt atgtcttcct aaactccaaa gtttctgcgt
    10861 caaatttcct attttcactg tagtctttga cacttcgaac gacaatttga atcatatatt
    10921 caaaaaaaaa tgaaaaattt gtgccaacaa gcctaaatat tctgaaaatt tgtttgattt
    10981 ttaatggata atgtgatata atggttgtca atctataatt cacataaaca gaggagtaga
    11041 aaatgaaaaa aaatcgtgta tttgctacag caggtcttgt tttattagca gcaggtgtac
    11101 ttgcagcatg cagttcttca aaatcatctg attcatcagc ccctaaagct tatggctatg
    11161 tttatacagc agacccagaa accttggact acctgatttc aagtaaaaat agtacaacag
    11221 tagtgacttc aaatgggatt gatggtttat tcactaacga taattacggt aatcttgctc
    11281 ctgcagttgc agaggattgg gaagtctcta aggatggttt gacctacact tataagattc
    11341 gtaaaggggt taaatggttt acctctgatg gagaagaata tgcagaggtg acggctaaag
    11401 atttcgtgaa cggtttaaaa cacgcagcag ataaaaaatc agaagctatg tatttagctg
    11461 aaaattcggt taaaggcttg gcagattatc tatcaggaac ttcaacagat ttttcaacag
    11521 ttggtgtcaa ggcggttgat gattatacgt tacaatacac tttgaaccag cctgaaccgt
    11581 tctggaactc taagttgacc tattctattt tctggcctct gaatgaagaa ttcgaaacat
    11641 caaaaggaag cgattttgct agaccaacag atccgacatc cttgctttat aatggtccat
    11701 tcttgttgaa agggttgact gcaaaatctt ctgtagagtt tgtaaaaaat gagcaatatt
    11761 gggataaaga aaatgtccac ctagatacta tcaatctagc ttactatgat ggatcagatc
    11821 aggagtcgct agagcgtaac ttcactagtg gagcttatag ttatgcccgt ctttacccta
    11881 ccagctccaa ctattctaag gttgcagaag aatacaagga caatatctat tacacacaat
    11941 caggctctgg gattgctggt ctgggtgtga atattgatcg ccaaagttac aactatactt
    12001 ctaaaactac agattcagag aaagtagcta ctaagaaggc attgcttaac aaagatttcc
    12061 gtcaagcctt gaattttgct cttgatcgct cagcttactc agctcaaatc aatggtaaag
    12121 atggagcagc tttagcagtt cgtaatttat ttgtaaaacc agactttgtt tcagctggtg
    12181 agaagacctt tggtgattta gtcgctgctc aacttcctgc ttatggtgat gagtggaaag
    12241 gtgtgaattt agctgatggg caggatggtt tattcaatgc tgacaaggcc aaggcagagt
    12301 ttgcgaaagc taagaaagct ttagaagcag acggcgttca gtttcctatt catctggacg
    12361 ttccagtaga ccaagcatca aaaaactaca tatctcgtat tcagtccttt aaacaatctg
    12421 tagaaacagt tcttggtgtt gaaaatgtcg ttgttgatat tcaacaaatg acaagtgatg
    12481 aattccttaa tattacttac tatgctgcca atgcttcatc tgaggattgg gatgtatcag
    12541 gaggagtttc atgggggcca gactatcaag acccatctac ttacctggat attttaaaaa
    12601 caactagcag tgaaactaca aaaacatatt taggatttga taatccaaat agcccttcag
    12661 tagttcaagt tggtttgaaa gaatacgata aattagttga tgaagctgcc aaagagacaa
    12721 gcgacttgaa tgtccgttat gaaaaatatg cagcggctca agcatggttg acagatagtt
    12781 cactctttat tcctgctatg gcttcttctg gtgcagcacc agtgctttca cgaattgttc
    12841 catttactgg agcttctgcg caaacaggct ctaaggggtc agatgtttac ttcaaatatt
    12901 tgaaattaca agataaagtg gtgactaagg aagagtatga aaaagctcgt gaaaaatggt
    12961 tgaaagaaaa agctgaatca aatgagaaag ctcaaaaaga attggcaagt catgtgaagt
    13021 aaataatttc taatagaact ttctctccat gaggagaagg ttcttttggg atttttaaag
    13081 gaaataatat gaaaaaatat atttttatgc gtgttttgcg gtcattggtt tcgattttct
    13141 tagtaacgac tttgacctac acgattatct ataccttggt tcctcgaaaa ttgattttca
    13201 agcaggatcc taactataat aaaattgcga caacggctga taaacgtgat aactatgaaa
    13261 atactgtgtt tgagcgtatg ggctacattg agtattacga tactaaagag ttgcaagaaa
    13321 aggcaagtag catggattct tctgtaacag tagaagcaaa tgcgaccaat aaagctattt
    13381 atgaaaagta catcaatcaa ttaggtcatg gttggacttt gggagaattt actgaaagtg
    13441 gtcaattcta tgctactcgt gaaattccaa tttttgaacg tgtttttcac ttctatgcta
    13501 acttgattga cattgaccat acaaataaaa tccaagaccc tgaaaatcca gacttgaaac
    13561 gctaccttcg ttttgaaaat gatccagcta tcggatggtc attggtcggt tcaggaacta
    13621 aacataaata tctcttgtac tttaacagtc agttcccatt tgttcatcaa aactttgtga
    13681 acttgaattt aggtgactct tacccaacct atgctaatac accagttctt caggttatta
    13741 ctcaaggtca aggacaaacc aaaactgccc aagttcagtt cccaacaggt aagaaaacgt
    13801 cttctgtaaa tatttactca agaacctaca agtcacctag tcaggctgac tctcgtgaag
    13861 tagctagcta tgggaaagat gatccttata cagcgactga aagtaattac caatatccat
    13921 ctatgattgt cagctctgct attactggtt tgattggttt ggttcttgcc tatgctcttg
    13981 ccgtgccact tggttcagcc atggctcgtt tcaagaacac ttggattgat agcctctcaa
    14041 cagggacttt gaccttcttg cttgctcttc caacgattgc cttggtttac atcgttcgat
    14101 tgattggatc atctattgcc cttccagatt cattccctat cttgggagct ggagattggc
    14161 gttcttacgt tttaccagca gtcatccttg gtttattggg tgctcctgtt acagccattt
    14221 ggattcgtcg ttacatgatt gacttgcaat ctcaagactt tgttcgtttc gctcgtgcaa
    14281 aaggtttgtc tgaaaaagaa atttcaaaca aacacatctt taaaaatgcc atggttccgc
    14341 tggtttcagg aattcctggt gccattattg gggttatcgg tggtgcaacc cttactgaaa
    14401 cagtcttcgc cttcccaggt atgggtaaaa tgttgattga ctctgtaaaa gcatctaata
    14461 actctatggt cgttggtctt gtcttcatct ttacatgtat ttctatcttc tcacgtcttt
    14521 tgggagatat ttggatgact attattgacc cacgtattaa attgactgag aaaggaggca
    14581 aataatgtct acaatcgata aagaaaaatt tcagtttgta aaacgtgacg attttgcctc
    14641 tgaaactatt gatgcgccag catattctta ctggaaatca gtgtttaaac aatttatgaa
    14701 gaaaaaatca actgtagtca tgttgggaat cttggtagcc atcattttga taagtttcat
    14761 ctacccaatg ttttctaagt ttgatttcaa tgatgtcagc aaggtaaacg actttagtgt
    14821 tcgttatatc aagccaaatg cggagcattg gttcggtact gacagtaacg gtaaatcgct
    14881 ctttgacggt gtctggttcg gagctcgtaa ctccatcctc atttctgtga ttgcgacagt
    14941 gattaacttg gttatcggtg tttttgtcgg tggtatttgg ggtatttcaa aatcagttga
    15001 ccgtgtcatg atggaagttt acaacgtcat ctcaaacatc ccacctcttt tgattgttat
    15061 tgtcttgact tactcaatcg gagctggatt ctggaatctg atttttgcca tgagcgtaac
    15121 aacatggatt ggtattgcct tcatgattcg tgtgcaaatc ttgcgctatc gtgacttgga
    15181 atacaacttg gcgtcacgta ctttgggaac accaaccttg aagattgttg ccaaaaatat
    15241 catgcctcaa ttggtatctg ttattgtgac aaccatgact caaatgcttc caagctttat
    15301 ctcatacgaa gccttcttgt ctttcttcgg tcttggatta ccgattacag tgccaagttt
    15361 gggtcgtttg atttcggatt attcacaaaa cgtaacaacc aatgcttact tgttctggat
    15421 tccattgaca acccttgtct tggtatcctt gtcccttttc gtagttggtc aaaacttagc
    15481 ggatgctagt gatccacgta cacatagata ggagtagaaa tgacaaaaga aaaaaatgta
    15541 attttgactg ctcgcgatat tgtcgtggaa tttgacgttc gtgacaaagt attgacagcc
    15601 attcgcggcg tttcccttga actagtcgaa ggagaagtat tagccttggt aggtgagtca
    15661 ggatcaggta aatctgtttt gacaaagacc ttcacaggta tgctcgaaga aaatggtcgt
    15721 attgcccaag gtagtattga ctaccgtggt caggacttga cagctttatc ttctcacaag
    15781 gattgggaac aaattcgtgg tgctaagatt gcgactatct tccaggaccc aatgactagt
    15841 ttggacccca ttaaaacaat tggtagtcag attacagaag ttattgtaaa acaccaagga
    15901 aaaacagcta aagaagcgaa agaattggcc attgactaca tgaataaggt tggcattcca
    15961 gacgcagata gacgttttaa tgaataccca ttccaatatt ctggaggaat gcgtcaacgt
    16021 atcgttattg cgattgccct tgcctgccga cctgatgtct tgatctgtga tgagccaaca
    16081 actgccttgg atgtaactat tcaagctcag attattgatt tgctaaaatc tttacaaaac
    16141 gagtatcatt tcacaacaat ctttattacc cacgaccttg gtgtggtggc aagtattgcg
    16201 gataaggtag cggttatgta tgcaggagaa atcgttgagt atggaacggt tgaggaagtc
    16261 ttctatgacc ctcgccatcc atatacatgg agtctcttgt ctagcttgcc tcagcttgct
    16321 gatgataaag gggatcttta ctcaatccca ggaacacctc cgtcacttta tactgacctg
    16381 aaaggggatg cttttgcctt gcgttctgac tacgcaatgc agattgactt cgaacaaaaa
    16441 gctcctcaat tctcagtatc agagacacat tgggctaaaa cttggcttct tcatgaggat
    16501 gctccaaaag tagaaaaacc agctgtgatt gcaaatctcc atgataagat tcgtgaaaaa
    16561 atgggatttg cccatctggc tgactaggag gaaggaaatg tctgaaaaat tagtagaaat
    16621 caaagattta gaaatttcct tcggtgaagg aagtaagaag tttgtcgcgg ttaaaaatgc
    16681 taacttcttt atcaacaagg gagaaacttt ctcgcttgta ggtgagtccg gtagtgggaa
    16741 aacaactatt ggtcgtgcta tcatcggtct aaatgataca agtaatggag atatcatttt
    16801 tgatggtcaa aagattaatg gtaagaaatc gcgtgaacaa gctgcggaat tgattcgtcg
    16861 aatccagatg attttccaag accctgccgc aagtttgaat gaacgtgcga ctgttgatta
    16921 tattatttct gaaggtcttt acaatcaccg tttatttaag gatgaagaag aacgtaaaga
    16981 gaaagttcaa aatattatcc gtgaagtagg tcttcttgct gagcacttga ctcgttaccc
    17041 tcatgaattc tcaggcggtc aacgtcaacg tatcggtatt gcccgtgcct tggtcatgca
    17101 accagacttt gttattgcag atgagccaat ttcagccttg gacgtttctg tacgtgccca
    17161 agtcttgaac ttgctcaaaa aattccaaaa agagctcggc ctgacctatc tcttcatcgc
    17221 ccatgacttg tcggttgttc gctttatttc agatcgtatc gcagttattt acaagggtgt
    17281 tattgtagag gttgcagaaa cagaagaatt gtttaacaat ccaattcacc catatactca
    17341 agccttgctt tcagcggtac caatcccaga tccaatcttg gaacgtaaga aggtcttgaa
    17401 ggtttacgac ccaagtcaac acgactatga gactgataag ccgtctatgg tagaaatccg
    17461 tccaggtcac tatgtttggg cgaaccaaac cgaattggca cgttatcaaa aaggactaaa
    17521 ctagtaatgg ttttataatt tccatgtcaa tttttatgga aattaggctc tttgtcaact
    17581 gtagtgggtt gaagtcagct aagctcgaga aaggacaaat ttcgtccttt cttttttgat
    17641 gttcaaagcg ataaaaatcc gttttttgaa gttttcaaag tttcgaaaac caaaagcatt
    17701 gcgcttgata agtttgatga gattattggt cgcttccagt ttggcattag aatagtgtag
    17761 ttgaagggcg ttgataatct tttctttatc tttgaggaag gttttaaaga cagtctgaaa
    17821 aataggatga acctgcttaa gattgtcctc gataagttcg aaaaatttct ctggttcctt
    17881 attctggaag tgaaaaagca agagctgata gagctgatag tggtgtttca agtcttccga
    17941 ataactcaaa agcttgttta aaatctcttt attggttaag tgcatacgaa aagtaggacg
    18001 ataaaattgc ttatcactca gtttacggct atcctgttgt atgagcttcc agtagtgctt
    18061 gatagccttg tgttcatggg attttcgatg aaactgattc atgatttgga cacgcacacg
    18121 actcatagca cggctaagat gttgtacaat gtgaaagcga tcaagaacga ttttagcatt
    18181 cgggagtgaa acagtct
//
LOCUS       DAVKMU010000038        17598 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKMU010000038 DAVKMU010000000
VERSION     DAVKMU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.038"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(162..365)
                     /locus_tag="VW343_001529"
     CDS             complement(162..365)
                     /locus_tag="VW343_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9756061.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(358..1440)
                     /locus_tag="VW343_001530"
     CDS             complement(358..1440)
                     /locus_tag="VW343_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9756062.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTIFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     IIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1566..2348)
                     /locus_tag="VW343_001531"
     CDS             complement(1566..2348)
                     /locus_tag="VW343_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9756063.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2348..3691)
                     /locus_tag="VW343_001532"
     CDS             complement(2348..3691)
                     /locus_tag="VW343_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9756064.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3803..5119)
                     /locus_tag="VW343_001533"
     CDS             complement(3803..5119)
                     /locus_tag="VW343_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9756065.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5313..6539
                     /locus_tag="VW343_001534"
     CDS             5313..6539
                     /locus_tag="VW343_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9756066.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGILGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6738..8312
                     /locus_tag="VW343_001535"
     CDS             6738..8312
                     /locus_tag="VW343_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9756067.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8576..9364
                     /gene="codY"
                     /locus_tag="VW343_001536"
     CDS             8576..9364
                     /gene="codY"
                     /locus_tag="VW343_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9756068.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9364..9939
                     /locus_tag="VW343_001537"
     CDS             9364..9939
                     /locus_tag="VW343_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9756069.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFSPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10021..10106
                     /locus_tag="VW343_001538"
     tRNA            10021..10106
                     /locus_tag="VW343_001538"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10055..10057,aa:Leu,seq:aag)
     gene            10389..11519
                     /gene="ugpC"
                     /locus_tag="VW343_001539"
     CDS             10389..11519
                     /gene="ugpC"
                     /locus_tag="VW343_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9756070.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(11666..11812)
                     /locus_tag="VW343_001540"
     CDS             complement(11666..11812)
                     /locus_tag="VW343_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756071.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            12020..12778
                     /locus_tag="VW343_001541"
     CDS             12020..12778
                     /locus_tag="VW343_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9756072.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            12865..13377
                     /locus_tag="VW343_001542"
     CDS             12865..13377
                     /locus_tag="VW343_001542"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9756073.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13559..14503
                     /locus_tag="VW343_001543"
     CDS             13559..14503
                     /locus_tag="VW343_001543"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9756074.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14512..15189
                     /locus_tag="VW343_001544"
     CDS             14512..15189
                     /locus_tag="VW343_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9756075.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLIKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15287..16045
                     /locus_tag="VW343_001545"
     CDS             15287..16045
                     /locus_tag="VW343_001545"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9756076.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16083..17588
                     /gene="lytC"
                     /locus_tag="VW343_001546"
     CDS             16083..17588
                     /gene="lytC"
                     /locus_tag="VW343_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9756077.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
BASE COUNT         5281 a         3172 c         3923 g         5222 t
ORIGIN      
        1 ccctgtgcaa tacagaacta aatccttcgg ataaattaat tgtctaactt tttggggggc
       61 agttcattct acacccacta ttatgcgttt tattgtggga agattttcct cattttctag
      121 tgaaattgaa ctttttctaa catcttttga tagatgactt cttaatcaat tatcatttcc
      181 attgttgttt cttggggttt catcccttgt ctttgataaa agcggagagc accctcgtta
      241 tcattccaga cacttagggt tagattattg cagccaattt ctttagcata atagcagaaa
      301 aatgacaata aaatagcccc tagatatttt gaaaggaaag cataggggct atatagttta
      361 atcaaaataa agcaaatctt tgctggtgct tgtaaagagg tcgaagccat ccttggtaac
      421 aacaccgcag tcttcaatac gaacaccgac tttaccaggg atatagatac ctggttcaac
      481 agagaagcac atgccttctt cgatgaccat gtcgtttcct tccatgatag atgggaattc
      541 atggacatcc ataccgatac catgcccgag acggtggttg aagtactcac cataaccagc
      601 tttttcgatg atctcacggg cagcgcggtc cacttcatga gcagtcacac ctggcttgat
      661 aaagtcaaga gcagcttgtt gggcttcaag agtcaagttg taaatatctt tcttgaattg
      721 gtctggtttg ccgacagcga ctgtacgagt catatctgac gcatagccat tgaccagaac
      781 ccccaggtca aagaggagaa gagcatcatt ttcaacctta ttagctgctg gaatgccgtg
      841 tggattcgca gcattatcac cagtcaagac catggtatca aagctcattt cgtaaccttc
      901 acgtttcatg gcaaagtcaa tttgtgcgat gatatctgtc tcagtcttat caagagaaat
      961 attgtcaaaa ccaacatgaa cagccttgtc agcataaaga cctgcaacca tcattttttg
     1021 tacttcatca gctgatttga tgaggcgcat gcgttggata cgaggagtga ggttttcaaa
     1081 ctcagcagtt tcaaaaattg ttttcaaacc atggtatttg gtcaagatga gattgtcaaa
     1141 ctcaacagcg acacgtttga agtcaagttg tggaagagca tgtttgattt tttgccatgg
     1201 attttcagaa tcgacatagc ccactactgg gaaggaaacg gtgctacttg cacgttctac
     1261 ttcaagagct gggacaaaga ggagaggttc ctgatccgct aggacaaaga ggaacatttg
     1321 gcgttcatgg ggatcactgt aaaaaccagt gaggtaattg attgtgacgg ggtcagatac
     1381 gacagcgacg tctagttttt ctgattcaag atatgttagg atttgttgta atttagacat
     1441 atgctacctt ctttctaccc ctctattttg gcaaaaaatg agagaaaatg caagggagaa
     1501 gggtaaaaga aaagacaaaa aactccgaca agataacgga gttctttgat atcattttcc
     1561 tttgtttaag aaaagtcagc cttgcttttg acgtcactgt actcttcagc gatttcttgg
     1621 ctgaggatat cctcataggc agggagttgg atatcctgac catatttctt cttgagggca
     1681 gtagtaacta ggcgataagc cagattggca ttacgagaga ggataggccc gtggaagtag
     1741 gaaccaaaga cattcttata atgaacccct tcaccgacct tttcttcgtt gtttccattt
     1801 ccatagacaa cctgtcccag cggtttttgg tcatcagaga ggaaggtacg accttggtga
     1861 ttttcaaatc catagtaggt ttcatcgaaa tcttcattgt gaatcttgat gtcaccgata
     1921 aaacggttat tggtctggtt gagcgtgtag tgtcccatga cccctagccc ttcgatacgt
     1981 tttcctgaag cttcaacata atattgaccc aatagttgga aaccaccgca gatagccaga
     2041 actacaccgt cgttttggat gtagttgtca atgctctctt ttttagcagg taggtcgtct
     2101 gcaatgatac tttgttcaaa gtcttgacca ccaccgaaaa aggcgatgtc gtagtgattt
     2161 tcatcaaagt catcatggag agaaacgatg tcaacggtca catgggctcc cagtttttca
     2221 gccacatact tgagcatgag gatgtttcca ttgtccccgt aggtattcat gagatttccg
     2281 tagaggtggg caatgttgag ctgatagggg taattgccat cttttgagga aagtgaagta
     2341 taaaccatta gttcatctcc tttctaacaa tctgacgact agccagcagt tcacgaaatt
     2401 ccagcatggc agtataagtt gccagaatat aggcatgctt gcagtcttga ttctcaatgg
     2461 tcttgagaac ttgctccaga ttactcgttt cagtgatttt ctcagctgga tagccagtca
     2521 ctcggaggcg acgagcgatt tcagaatgac gaacaccgcc agcgttgatt tcaggaatgt
     2581 ccatgtcagt gatttgttca aagtctgcat cccagatcca gctagtgtca attccatctg
     2641 catagttggc attaaggagg acagataggc taaatggata aggtgctagt ttgatcattt
     2701 cgatagcttg ggttgcaccg actggatttt taatcaagac aagggtacat tccttgtcac
     2761 cgatatgaaa ggtttcttgg cgtccaaaga cagcacggct cttgtcaaat ccctgtttga
     2821 tgagttgcga atcggcacct aggaaacggg cgatggccac agcagctagg gcgttataga
     2881 tattatagag cccgccgatt tggataccgt attcttggcc gtctatgaca aagcgagagc
     2941 gattgttggt caactcaacc agttttgtca aacgatagtc gagatcagga cgtttacatc
     3001 cacaaccttc acagatatag gcacccaagt ttgcataggt attatgctca tatttgagga
     3061 tgccttggca gtcaggacag agaatccctt cggtattgta gtgagccagt tgggctggtc
     3121 ccttttccaa gtcaaaacca aaatactcta tagggtttgg aatagttggc ttgtagaaaa
     3181 gtggactgtc tccgttaagg agaacagtag cagttggaac tttccgaatg gcatccaata
     3241 tcatgttata ggtagtatag atttcaccga aacggtccat ctggtcacgg aagatattag
     3301 taatgacaaa aagactaggc tggatatagt cacagatacg agatagactg gcttcgtcaa
     3361 tttcgaggac ggcaatattt ttcccagttt tagaagattt ggctgttagg aaggttgttg
     3421 caatccctgt aatcatgttg gcaccgcttg ggttggttag aacttgacca taaacctctt
     3481 ttaaaatgcc gacagtgagg gcagttgtca gggtttttcc atttgttcca gtgacaacga
     3541 caatctcgta gttcttagct aggttttgta aaatatcttt atcaaattga agggcgactt
     3601 tccctgggag cgtacttcca cgtccaagac ggcttaaaac gaagtgggaa gaacgcccag
     3661 caagaaggcc caaagtagtt tttaagttca tgtttctatt atatcacaaa agtatatcac
     3721 aaaagaggga aaagacagat ttggtgtttc gtagaagtaa aaacagccca aagagggctg
     3781 tcagttaaga tgagatttca acttaaatcg caaacaagtc attcaagttc tcagccaagg
     3841 ttgggtgagt gaagatttgt tttgtgaagt aagtgtaagg aatcttgttg tccatagcaa
     3901 cagtaataat gttgatgatt tcttgagaac cttctgagaa gatgcttgca ccaagaattt
     3961 cttttgtttc agtattaaca acagctttga aagctccgcg aaggtctcca tttacgtgac
     4021 cacgaggcat ggctgcaaca gggatttctt tcactgcgta tggaagtttc aaatcagctg
     4081 cttggctttc agtcaaacca acttgtgaaa gtgcaggtgt gatgaacata gtatttggca
     4141 cattgagacg gtcctcaagt gtgtagctgc catctccagc aaggtagctg taaacaacac
     4201 ggaagtcatc aagtgaaatg taagtaaatt gaagaccacc gttgacatct ccaactgcaa
     4261 agacaccagg aacgtttgtt tgacagtgtt tatctacttt aatagcgcca cgttcagtta
     4321 gttcaatatc tgtattttca agttgaagtg gttctacatt tggtttgcgt ccagttgcgt
     4381 agagaagggc gtcgaaacgg taagtttcgt cttcagttac gacaagcact tggtcaccgt
     4441 cgtttttaat ttcagtagta tggatatttt gaagcaattc aataccgtct tcttccatgt
     4501 attgtttagc aagagctgcg atggaaggtt ctgcacgagg taggaatgta tccaaggcat
     4561 ctaggactgt aaccttgctt cctagtttat tgtaaaggcc agcaaattca agaccgatat
     4621 ttccgccacc aaggactcca agtttttcag gcaatttatc caagctttgg atacctgttg
     4681 agtcaaagac gtttttgctt gtagcaagtc cagggattgg caagacgttt gaaacagcac
     4741 cagtgttgat gacgatagtt tcagcagtca attcttgttt ttcatcacca gcttggattt
     4801 cgatgacttt atttgaaagg aagtgagctt cagcatcaaa gatatcgacg ccagtaccag
     4861 caacagtcgt atagttttta ccgttgaggc gaccagtgat cgtgtttttg gtagcaatga
     4921 cttcttcaaa agacaagtct ttctcagcag caaccagcaa agttttagtt gggatacaac
     4981 cgatgttgat acaagttcca ccgtacatag ctttgctacg ttcaacgagg gcaacttttt
     5041 tgccagctga agccaattta ccagctagtg ttttaccagc tttaccaaat ccgataacga
     5101 ttaaatcata agttaacatt tagaattcct cctattcgaa ttacattcta gcacaagaaa
     5161 aaaacttttg cacaattctg ctacgaaaaa gacagaaaaa tatgaaaacg cttttcaggc
     5221 aaaaaactag taattttttg acaagcttga taaactaaaa tcctatctat ttttatataa
     5281 gatagaataa atgttcgaaa ataggaggtt ttatgaaatc gaatcgttat attattgcct
     5341 ttgctggggt cattttacac ttaatgctgg gttcgactta tgcctggagt gtttatcgta
     5401 accctattat tgaaaaaacg ggatgggatc aggcttctgt tgccttcgcc tttagtctag
     5461 caatcttttg tttgggctta tcggctgcat ttatggggcg tttggtagaa aaatttggtc
     5521 cgagagtcat ggggagtcta tctgcttttc tatacgcagg tggaaatatc ttaacaggat
     5581 ttgcaataga ccgtcaggaa ctgtggttgt tgtatctagc ttatggcatt ttaggtgggc
     5641 ttggtttggg agcaggctat attacccctg tgtcaacgat tataaaatgg tttcctgata
     5701 aacgtggtct cgcaacaggt ttagcgatta tggggtttgg ttttgcttct ttattgacta
     5761 gtcccatagc gcaacatctc atcgcagggg tagggcttgt agaaactttt tatattttag
     5821 gtgcaagtta ctttattatc atgctcctag cttcacaatt cattaagcgt ccaaatgagc
     5881 aagagcttgc aattttatct gcttcaggga aagaaaaaac agactctctg acgcaaggga
     5941 tgactgcaaa tcaggctcta aaaagcaatc ggttttatat gctttggatt attttcttta
     6001 tcaacatagc ttgtggttta ggcttaattt cagcggcatc gccaatggca caggagatgg
     6061 ctggcttgtc tacaagtcat gcagcagtaa tggtgggtat tttggggatt ttcaatggat
     6121 ttggtcgctt gctctgggcg agtttgtctg actatatcgg tcgccctcta acctttagta
     6181 tattactgct tgtcaatctt ttcttttctc tctcactttg gctctttaca gattccgttt
     6241 tatttgtagt tgctatgtct attttgatga cttgctatgg agctggtttt tctttgattc
     6301 cagcttatct cagtgatatt tttggaacca aggaattggc cgctctgcat ggttatattt
     6361 taacagcttg ggcaatggct ggtttagcgg gacctatttt attagcagag acttataaaa
     6421 tggctcattc gtacacacaa accttgttcg tttttctcat tttatacagt atcgccttgg
     6481 ctttgtctta ttatctaggt cgttcaatca aaaaagaaag tcaaaaaccg cttacatgat
     6541 tttctgtaga aatggggcta tcttttgctc tggtcttgtg ttatactaga taggttgcaa
     6601 agaaaacagt acttttcttt tgtggaaaag aagcaacacg attttatatc tagttatatg
     6661 aaaatacgtc ataaaaagaa aagtataaac taagccctat agggtggctt cgcaccacct
     6721 ttagaaagaa gaataacgtg aaatttaatg aattaaactt gtctgctgat ttgctagcag
     6781 aaattgaaaa agctggtttt gtagaagcta gtcctatcca agaacaaact attcctttgg
     6841 cccttgaagg caaggatgtt atcggtcaag ctcagactgg tacaggaaaa actgcagcct
     6901 ttggcttgcc tacccttgaa aaaatccgta cagaagaagc gactatccaa gctttggtca
     6961 tcgctccaac tcgtgaacta gctgtccaaa gtcaagaaga actcttccgc tttggtcgta
     7021 gtaagggagt caaagtccgt tcagtatatg gcggatcaag cattgaaaaa caaattaagg
     7081 ctcttaaatc tggtgcccat atcgtggtgg gaactccagg tcgcctcttg gacttgatta
     7141 aacgcaaggc cttgaaatta caagacattg aaacccttat ccttgacgaa gcggatgaaa
     7201 tgcttaacat gggcttcctt gaagacatcg aagccattat ttcccgtgta cctgagaacc
     7261 gtcaaacttt gcttttctca gcaactatgc cagatgccat caaacgtatc ggtgttcagt
     7321 ttatgaaagc ccctgaacat gtcaagattg cggctaagga attgacaaca gaattggttg
     7381 accagtacta tatccgtgtt aaggaacaag aaaaatttga caccatgact cgtctcatgg
     7441 atgtggcaca accagaactc gctattgtat ttggtcgtac caaacgccgt gtggatgaat
     7501 tgactcgtgg tttgaaaatt cgtggcttcc gtgcagaagg aattcatggc gacctagacc
     7561 aaaacaaacg tcttcgtgtc cttcgtgact ttaaaaatgg caatcttgat gttttggttg
     7621 cgacagacgt tgcagcgcgt ggtttggata tttcaggtgt gacccatgtc tacaactacg
     7681 atattccaca agatcctgag agttatgttc accgtatcgg tcgtacaggt cgtgctggta
     7741 agtcaggtca atctattact tttgttgctc caaacgaaat gggttacctt caaatcattg
     7801 aaaacttgac taagaaacgc atgaaaggtc tcaaacctgc aagtgtagaa gaatccttcc
     7861 aatcaaaaaa acaggtagct ctcaagaaaa tcgaacgtga ttttgcagat gaaaccattc
     7921 gtgccaactt tgagaaattt ggtaaggatg ctcgcaaatt ggctgctgag tttactccag
     7981 aagaattggc aatgtatatc ttgagtctga cagtccaaga cccagatagc cttccagaag
     8041 tggagattgc acgtgaaaaa ccactaccgt ttaaaccatc aggtaatggt ttcggtggta
     8101 aagctaaggg aggtcgtgga ggccgtcgtg gggacgaccg tcgagagcgt gatcgccgtg
     8161 gcaatggtcg ccgtgatgag ttcaaaaaag gaagtcgtgg caacgatcgt tttgataagg
     8221 aaaaacgtta ccgtaaggat aataaaaaac cacgcaatac tttaagcgaa aagcaaacag
     8281 gctttgttat tcgtaacaag ggcgataaat aagaaaaagc ggttcaaaat gaatcgcttt
     8341 tttatataag gagaccgaat gtgaaaagac aaataaaatt attatattat cttttataat
     8401 gttatgatac acaaaaatag gcaacttgtc aatagaaaag gaacaattta attaaaaata
     8461 ttctggtttt taattaaatt gtaagtcaat gcaagtcaat aaggaatttt cagataatta
     8521 ttgaaaaaga gacgttttta tgttataatg atagcgatta aattcgaggt gaaaaatggc
     8581 acatttatta gaaaaaacta gaaaaattac atcaattttg aaacgctcag aggagcagtt
     8641 gcaggatgag cttccttaca acgctattac gcgtcagtta gcggatatta ttcattgcaa
     8701 tgcctgcatt atcaatagta agggacgtct gcttggctat tttatgcgtt ataaaacaaa
     8761 tacagatcgc gtagagcaat tcttccaaac taagattttc ccagatgact acgttcaagg
     8821 ggctaatatg atttacgaaa cagaagcaaa cttgcctgtt gagcatgata tgagtatttt
     8881 ccctgttgag agtagagatg attttccaga tggcttgacg actattgcac cgattcatgt
     8941 atcggggatt cgccttggtt ctttgattat ttggcgtaat gataaaaaat tcgaagatga
     9001 ggacttggtt cttgttgaga ttgccagtac cgttgttggg attcagcttc ttaacttcca
     9061 acgtgaagaa gatgagaaaa atattcgtcg tcgtactgct gtcaccatgg cggtcaatac
     9121 cctttcttac tccgaactcc gtgctgtttc agcaatttta ggggaattaa atggaaatga
     9181 agggaagttg actgcgtcag tgattgcaga tcgtatcgga atcactcgct ctgtgattgt
     9241 caatgctctt cgtaaacttg agtctgcggg gattattgaa agtcgctcac ttggaatgaa
     9301 gggaacctat cttaaggtct tgatttcaga tatttttgaa gaagtgaaga aaagagatta
     9361 ctaatgacaa aggctttaat ttcgattgat tatacagaag attttgttgc tgatagtggg
     9421 aaactaacag caggtgctcc agctcaggcg atttcggatg ccatcagcaa ggtaactcga
     9481 ttagcttttg aacgaggaga ttatatcttc tttactattg atgctcatga agaaaacgat
     9541 tgtttccatc cggaaagtaa gttattttct cctcataatc tgattgggac gagtggacgg
     9601 aatttatatg gagatttggg gatcttttat caagagcatg gttcagacag tcgtgtcttt
     9661 tggatggata aacgccatta ctcagctttt tcagggactg acctagatat tcgtttgaga
     9721 gagcgtagag tgtctaccgt tatcttaaca ggtgtcttga cggatatctg tgtcctacat
     9781 acagctatag atgcctataa tctaggatat gacatcgaga ttgttaaacc agctgttgct
     9841 tccatctggc ctgaaaatca tcaatttgcc ctaggtcatt tcaaaaatac actcggagct
     9901 aagttagtag atgaaaatct aaatgaactt tctgagtaac ctggttggtg aaaatgaaaa
     9961 aatttgaaaa aaagtgttga caagcatccc aaaagttgat atactagtaa agtaatcgat
    10021 gcggggatgg cggaattggc agacgcgcag gactaaggat cctgtgaccg ctttaggtcg
    10081 tgagggttca agtccctctc tccgcatagg aaaagagtta gctaaagcta gctcttttgt
    10141 atttctctaa aaaattagaa tccagtcaaa tgtagtggag aatcagcgaa aatatgggaa
    10201 aactgatcct tgtttctgct tttgtcatag aagaataata aagattatct tcttcaaatt
    10261 ctccgatatt ctctaaagtt ttgtgcaagt tgcacagaac ttttttattt ttttggtcat
    10321 cttgccatag agatataaag cgttttcatc tataatataa ttatcaaaag acaaaaggag
    10381 ttcacctcat ggtagaattg aatcttaaaa atatttacaa aaaatatcca aacagcgaac
    10441 actattcagt tgaagatttc aacttgaaca tcaaagataa agaatttatc gttttcgtag
    10501 gaccttcagg atgtggtaaa tcaactacac tccgtatgat tgctggtctt gaagacatta
    10561 cagaaggtac tgcatctatc gatggcgtgg ttgtcaacga cgtagctcca aaagaccgtg
    10621 atatcgccat ggtattccaa aactacgctc tttacccaca catgactgtt tatgacaaca
    10681 tggctttcgg tttgaaattg cgtaaataca gcaaagaaga cattaacaaa cgtgttcaag
    10741 aagcagctga aatacttgga ttgaaagaat tcttggaacg taaaccagct gacctttcag
    10801 gtggtcaacg tcaacgtgtt gccatggggc gtgcgattgt ccgtgatgcg aaagtattct
    10861 tgatggacga acctttgtca aacttggatg ccaaacttcg tgtatcaatg cgtgctgaaa
    10921 tcgctaaaat tcaccgtcgt atcggagcta caactatcta tgtaactcac gaccaaacag
    10981 aagcgatgac acttgcagac cgtatcgtta ttatgtcagc tactaagaac cctgctggta
    11041 caggtactat cggacgtgta gaacaaatcg gtactcctca agaagtttac aaaaatccag
    11101 ttaacaaatt cgttgcagga ttcatcggaa gcccagctat gaacttcatc accgtgaaat
    11161 tggttggtag cgaaattgtt tctgacggtt tccgtttgaa agtgccagaa ggagcattga
    11221 aagttcttcg tgaaaaaggc tacgaaggaa aagaattgat ctttggtatc cgtccagaag
    11281 acgtgaatgc agaacctgct ttccttgaaa cattcccaga ctgtgttgta aaagcgacta
    11341 tctctgtatc agaactgctt ggttcagaat ctcaccttta ctgccaagtt ggtaaagacg
    11401 agtttgttgc aaaagttgat gctcgtgact acttgcaaac aggtgcaaca gttgagcttg
    11461 gatttgactt gaacaaagca cacttcttcg atgtagaaac tgaaaaaaca atctactaaa
    11521 ataaataaaa ttcaaagcac tacaagaaaa gatatctctt tatcaattgt agtggagaga
    11581 tatcagttaa tctagggaga gaaacaaaat gcttctctcc tttttgctag ggaagtcata
    11641 ttatgcatct atattgtgat gctctttaat actcttcgaa aatctcttca aaccacgtca
    11701 acgtcgcctt gccgtacgta tgattactga tttcgtcagt tttatctgca acctcaaaga
    11761 tgtactttga gcagcttacg gctagtttcc tagtttgctc tttgatttcc attgagtatt
    11821 atttgtgggt accatctaca agtgaagcta tatgcgtaaa ctacgtgagc aattgaattc
    11881 gaactagaga ggtaataata aatttatgct atagttatgg tgacttgtat gcttttgatt
    11941 ctagtttatc aaataataga ttagaattgt cagataatat cattttgtgt tataatgaag
    12001 aaaaaacaga ggtgttcaaa tgtcagaagc aggtcataag tttttagcaa aattggggaa
    12061 aaaacgctta cgtccaggtg gaaagcgtgc cacagattgg ttaattgcag aaggaggatt
    12121 ttcaaaagaa aagagaatac tagaggttgc gtgtaatagg ggaactacag caattgagtt
    12181 ggcacagcgt tttggttgca agataactgc tgttgatatg gatgctcaag ctttagaagt
    12241 ggctaaaaaa tctgctggaa cggcaggtgt tgctcattta atcagttttg aaagagcaaa
    12301 tgcaatgaaa cttccttatc aagatgctag ttttgatatt gttataaatg aagctatgct
    12361 gactatgcaa gccgatcaag ctaagaaaaa atgtgtaatg gaatatctaa gggtattaaa
    12421 acctggaggt cttctcttga cacatgatgt gcttcttaag gaagctaaag agtctatcag
    12481 acaggaatta tcacaagcaa ttcatgtaaa tgtaggtcct ttaactcaag atggttggga
    12541 acaggtgatg atagaatcag gttattgtga tgtgaaagca ttgactggtg aaatgacatt
    12601 aatgaaatta tcgggtatga tttatgacga aggtttgcta ggaactttga aaatttgtgt
    12661 aaatgcttgt aaaaaggaga atagaaagca gtttttaact atgtataaaa tgtttgctaa
    12721 gaataaacag aaattgggct ttattgcgat ggctagttat aaatcgtcaa aacgttagat
    12781 aattattgaa gttaactttt ccttttttct ttcttaaaaa atatgctata atagagagta
    12841 aaaaactttg aaagaaagaa aaagatgaat ttaaaagatt acattgcaac aattgaaaat
    12901 tatccaaagg aaggcattac cttccgtgat attagtcctt tgatggctga tggaaatgct
    12961 tatagctacg ctgttcgtga aatcgttcag tatgctactg acaagaaagt cgacatgatc
    13021 gtgggacctg aagctcgtgg atttatcgtg ggttgtccag ttgcctttga gttgggaatt
    13081 ggttttgcgc ctgttcgtaa gccaggtaaa ttgccacgcg aagttatttc tgctgactat
    13141 gaaaaagagt acggtgtcga taccttgact atgcacgcgg atgccattaa gccaggtcaa
    13201 cgtgttctta ttgtagatga ccttttggcg acaggtggaa ctgttaaggc aactatcgag
    13261 atgattgaaa aacttggtgg tgttatggca ggttgtgcct tccttgttga attggatgaa
    13321 ttgaacggcc gtgaaaaaat tggtgactac gactacaaag ttcttatgca ttattaatga
    13381 aaacagtccc tagggctgtt ttctctacac taggatataa aaatagacta taactagtta
    13441 gagaaaaact ataattgaaa actatatctt cttgcagtat aataaaagga ctaagtgttt
    13501 gagatttgtc ttcaaacata tgcaattatt cctgaaagag tacagttagg agagggttat
    13561 gccgattcga attgataaaa aattgccagc tgttgagatt ttacggacag agaatatctt
    13621 tgtcatggat gatcaacgtg ctgcccacca agatatccgt cctttgaaga ttttaatttt
    13681 aaatctcatg ccacagaaaa tggtcacaga gacccagttg ttgcgccact tggctaatac
    13741 acccctacaa ctggatattg attttctcta tatggagagc caccgttcta aaacaactcg
    13801 ttcagagcac atggagacct tctataaaac ttttcctgaa gtcaaggatg agtattttga
    13861 tgggatgatc atcacgggtg ctccagttga gcatttacca tttgaggaag tggactattg
    13921 ggaggaattt agacagatgc ttgagtggtc taagactcat gtctattcga cccttcatat
    13981 ctgttggggg gctcaggctg ggctttatct gcgctatggt gtagaaaaat accagatgga
    14041 cagtaagcta tcaggtattt atcctcagga caccctaaaa gagggtcacc ttctatttag
    14101 aggctttgat gatagctatg tatcccctca ttcacggcac acggagattt ctaaggaaga
    14161 ggtcttaaac aagaccaatc tcgagatttt atcagaagga cctcaggttg gggtttctat
    14221 tttggccagt cgtgatttac gagaaattta tagttttggt catttggagt atgaccgtga
    14281 taccttggca aaagagtatt ttcgagatcg tgatgcaggt ttcgacccac atattccaga
    14341 aaattacttt aaagatgatg atgttaatca ggtaccttgt ctttgttggt cttcatctgc
    14401 agccctcttt ttcagtaatt gggtaaacca tgcggtctat caggagacgc cttttgattg
    14461 gagaaagata gaagatgatg catctgcata tgggtattta taagaggaat tatgacatat
    14521 ttagacgctt ttaaatcagg taccttggtt ttaccgagtg ccctgctctt gcattttaag
    14581 gaactctttc cttctagcga cgattttctg gtttggcaat ttttctattt gcaaaatacg
    14641 acaggcttag aagaaatgtc gccaagccag attgctgaaa ggattggcaa ggaaatttcg
    14701 gatgtcaacc agtccatttc taatctgacg gaaaggggac tgctccagta tcgtactatc
    14761 gaattaaatg gcgaaattga attgctcttt gatgctagtt tggccttgga acgtttggat
    14821 gacctgtttg gagcagttcg ttcaagttca gaccagctaa cacctcaaaa ccagctcaag
    14881 gatttggtgg aaaccttcca gcaggagttg ggacgattgt tgacgccttt tgagattgag
    14941 gatttgatca agacactaaa ggaagatgga accagtgctg acttgattaa ggaggctctt
    15001 cgtgaagctg ttttgaatgg aaaaccaaac tggaagtaca ttcaggcgat tttgagaaac
    15061 tggcgccatg aaggaatcaa gagtgtggct caaattgagg ccaagagagc agaaagagaa
    15121 gcaagcaatc ctcagttgac acaggtatct gcagatttca gaaatgccat ggatctctgg
    15181 aaggattaat ccatgcaagt aggcttgaaa tccgagtaag atttgcaagc tgtgtataat
    15241 tgtgatagaa taaatagaaa ataaattgaa aaaagaggta tgtgaaatgt cacgtaaacc
    15301 atttatcgct ggtaactgga aaatgaacaa aaatccagaa gaagctaaag cattcgttga
    15361 agcagttgca tcaaaacttc cttcatcaga tcttgttgaa gcaggtatcg ctgctccagc
    15421 tcttgatttg acaactgttc ttgctgttgc aaaaggctca aaccttaaag ttgctgctca
    15481 aaactgctac tttgaaaatg caggtgcttt cactggtgaa actagcccac aagttttgaa
    15541 agaaatcggt actgactacg ttgttatcgg tcactcagaa cgccgtgact acttccatga
    15601 aactgatgaa gatatcaaca aaaaagcaaa agcaatcttt gcgaacggta tgcttccaat
    15661 catctgttgt ggtgaatcac ttgaaactta cgaagctggt aaagctgctg aattcgtagg
    15721 tgctcaagta tctgctgcat tggctggatt gactgctgaa caagttgctg cctcagttat
    15781 cgcttatgag ccaatctggg ctatcggtac tggtaaatca gcttcacaag atgatgcaca
    15841 aaaaatgtgt aaagttgttc gtgacgttgt agctgctgac tttggtcaag aagtcgcaga
    15901 caaagttcgt gttcaatacg gtggttctgt taaacctgaa aatgttgctt catacatggc
    15961 ttgcccagac gttgacggtg cccttgtagg tggtgcgtca cttgaagctg aaagcttctt
    16021 ggctttgctt gactttgtaa aataatcagt aagtagcaaa agctaggtgg aacagcattc
    16081 agatgtctgt tacatttttt ataggagagg aaagattgaa aataaaaatt ggattagcaa
    16141 gtatctgttt actaggcttg gcaactagtc atgtcgctgc aaatgaaact gaagtagcaa
    16201 aaacttcgca ggatacaacg acagcttcaa gtagttcaga gcaaaatcag tcttctaata
    16261 aaacgcaaac gagcgcagaa gtacagacta atgctgctgc ccactgggat ggggattatt
    16321 atgtaaagga tgatggttct aaagctcaaa gtgaatggat ttttgacaac tactataagg
    16381 cttggtttta tattaattca gatggtcgtt actcgcagaa tgaatggcat ggaaattact
    16441 acctgaaatc aggtggatat atggcccaaa acgagtggat ctatgacagt aattacaaga
    16501 gttggtttta tctcaagtca gatggggctt atgctcatca agaatggcaa ttgattggaa
    16561 ataagtggta ctacttcaag aagtggggtt acatggctaa aagccaatgg caaggaagtt
    16621 atttcttgaa tggtcaagga gctatgatac aaaatgaatg gctctatgat ccagcctatt
    16681 ctgcttattt ttatctaaaa tccgatggaa cttatgctaa ccaagagtgg caaaaagtgg
    16741 gcggcaaatg gtactatttc aagaagtggg gctatatggc tcggaatgag tggcaaggca
    16801 actactattt gactggaagt ggtgccatgg cgactgacga agtgattatg gatggtgctc
    16861 gctatatctt tgcggcctct ggtgagctca aagaaaagaa agatttgaat gtcggctggg
    16921 ttcacagaga tggtaaacgc tatttcttta ataatagaga agaacaagtg ggaaccgaac
    16981 atgctaagaa aatcattgat attagtgagc acaatggtcg tatcaatgat tggaaaaagg
    17041 ttattgatga gaacgaggtg gatggtgtca ttgttcgtct aggttatagc ggtaaagaag
    17101 acaaggaatt ggcgcataac attaaggagt taaaccgtct gggaattcct tatggtgtct
    17161 atctctatac ctatgctgaa aatgagaccg atgctgagaa tgacgctaaa cagaccattg
    17221 aacttataaa gaaatacaat atgaacctgt cttaccctat ctattatgat gttgagaact
    17281 gggaatatgt aaataagagc aagagagctc caagtgatac agacacttgg gttaaaatca
    17341 tcaacaagta catggacacg atgaagcagg cgggttatca aaatgtatat gtctatagct
    17401 atcgtagttt attacagacg cgtttaaaac acccagatat tttaaaacat gtaaactggg
    17461 tagcggccta tacgaatgct ttagaatggg aaaaccctta ttattcagga gaaaaaggtt
    17521 ggcaatatac ctcttctgaa tacatgaaag gaatccaagg gcgcgtagat gtcagcgttt
    17581 ggtattaagc gatgattt
//
LOCUS       DAVKMU010000039        17309 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKMU010000039 DAVKMU010000000
VERSION     DAVKMU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.039"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..656
                     /locus_tag="VW343_001547"
                     /pseudo
     CDS             1..656
                     /locus_tag="VW343_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(720..1802)
                     /locus_tag="VW343_001548"
     CDS             complement(720..1802)
                     /locus_tag="VW343_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9756078.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(1799..2776)
                     /locus_tag="VW343_001549"
     CDS             complement(1799..2776)
                     /locus_tag="VW343_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9756079.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            2991..3368
                     /locus_tag="VW343_001550"
     CDS             2991..3368
                     /locus_tag="VW343_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9756080.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            3603..4994
                     /locus_tag="VW343_001551"
     CDS             3603..4994
                     /locus_tag="VW343_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9756081.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            5108..5950
                     /locus_tag="VW343_001552"
     CDS             5108..5950
                     /locus_tag="VW343_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9756082.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            6223..6984
                     /locus_tag="VW343_001553"
     CDS             6223..6984
                     /locus_tag="VW343_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9756083.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            7177..8094
                     /gene="glyQ"
                     /locus_tag="VW343_001554"
     CDS             7177..8094
                     /gene="glyQ"
                     /locus_tag="VW343_001554"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9756084.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            8306..10342
                     /locus_tag="VW343_001555"
     CDS             8306..10342
                     /locus_tag="VW343_001555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9756085.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            10384..10641
                     /locus_tag="VW343_001556"
     CDS             10384..10641
                     /locus_tag="VW343_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9756086.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(10864..12108)
                     /locus_tag="VW343_001557"
     CDS             complement(10864..12108)
                     /locus_tag="VW343_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9756087.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(12127..12732)
                     /locus_tag="VW343_001558"
     CDS             complement(12127..12732)
                     /locus_tag="VW343_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9756088.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            complement(12792..13715)
                     /locus_tag="VW343_001559"
     CDS             complement(12792..13715)
                     /locus_tag="VW343_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9756089.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            14009..15388
                     /locus_tag="VW343_001560"
     CDS             14009..15388
                     /locus_tag="VW343_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9756090.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            15540..16415
                     /gene="pdxS"
                     /locus_tag="VW343_001561"
     CDS             15540..16415
                     /gene="pdxS"
                     /locus_tag="VW343_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9756091.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            16416..17000
                     /gene="pdxT"
                     /locus_tag="VW343_001562"
     CDS             16416..17000
                     /gene="pdxT"
                     /locus_tag="VW343_001562"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9756092.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEDVEILATVDNQIVA
                     AQEKNMLVTSFHPELTDDAHLHQYFIEMCKEKRQ"
BASE COUNT         5259 a         3157 c         3812 g         5081 t
ORIGIN      
        1 ccatggttgg cgaaatatta tgccacgtcc agaacatcct aggaaagcag acgctcaaac
       61 cattgtcgcg tctaaaaata aaatctcaat tcaagaagac aagaaagcgc tttaaaacca
      121 gtagaccttt tcataaggtt cgcttgatgt accaagatga ggctggtttc ggtagaatca
      181 gtaaactggg atcttgttgg tctccaatag gagtaggtcc acatgtccat agtcactata
      241 tacgagaatt tcgctattgt tatggagctg ttgatgcccc atacaggcga atcatttttc
      301 ttaatagctg gtggatgtaa tactgagtgg atgaacgtct ttttagaaga gctttcacaa
      361 gcttatccag atgattattt tttactcgtt atggacaatg ctatatggca taaatcaagt
      421 accttaaaga ttccgactaa tattgatttt acctttattc ctccatacac accagagatg
      481 aacccattga acaagtgtgg aaagagattc gtaaacgtgg atttaagaat aaagcctttc
      541 gaactttgga agatgtcatg aatcaactcc aagatgtcat acaaggactg gagaaggagg
      601 tgataaagtc catcgttaat cggagatgga atagaatgct ttttgaaagc agatgagtat
      661 aaaattgaat tgcttataaa aaagctccat acactggatg tgtatagagc aatggggctt
      721 tatttgatat agagttcttg gttttttagg acaatttctc ggatacttgc aaacttttta
      781 agttttttga tttcttctgg atgagtgacg agagtaataa catatccttc cttgcccata
      841 cggccagtac ggccagcacg gtgtgtgtag gtttcgctat ctctaggaat atcaaagttt
      901 acgacacatt ctaggctatc gatatcaatt ccacgagcca aaaggtcagt tgcaagaagc
      961 agggttagtt ggttatcttt aaacttttct aagatgattt ttctaaattt aacattaaca
     1021 tcactagcga gggaaacagc caatatatca cgatactgta gtttttcctc ggcattccca
     1081 aggtctgaca ggctattgaa gaagactaga ccacggaaat cctctacatg agccagtttt
     1141 cgcagcatat ccactcgatg acgttggtct acctgcatgt agaaatgctg gatattgtcc
     1201 aatttttgat cagagagatc aatagtgcgt gtattcggca caatcttttc ttggtcaaac
     1261 ttggtcgtgg cactcatgta gaccagttgg tggtcacgag gtgcgtagtg agtgattttt
     1321 tctacaaagt gaatctgaga atcatctagt aattggtcaa attcatccag gatgatggtt
     1381 tccacattca tcatcttgat ttttttaagt ttaatgagtt caaagatacg gccaggagtt
     1441 ccaatcagaa tttctggccc ctttttaaga cgttcaattt gtcgtttctg acttgaacct
     1501 gaaaggaaga gttgagcagt caatccgata gcttctgccc acgttttaca tacatcaaaa
     1561 atctgtccag caagttccgt atttggtgct agaatcaaga gttgttgggc ttttttcttt
     1621 tgtagtctga gaagacttgg taggagatac gctagggtct taccagttcc ggtttggctc
     1681 actcctagga ggttttctcc agcaagaagg ggctcaaata gttgagtttg aatgggggtg
     1741 aattcttgga aaccgagttg gtcactcagt tcttgccatt cagtcggtag tttggttttc
     1801 atttttctgc ctcaaatcta atgccagcag tctggcgcat ggtatatagt agctcatgaa
     1861 cagagcctgc atcatacagc caagtttggt agagattcag atctggttgc tggatcatgt
     1921 gtgcaaatgc agcgacttcc tcagtcatcg tatgaggagc ctgttggata ggaagctgga
     1981 cttgatttcc ttggtggtcg gtaaaaatag ctgagcgaat atgctcaatc gtgttgagag
     2041 tcaaggttcc atctgttgta taaatctcgc aaggaagatt ggaagtgatg ttttttccag
     2101 ccttgatgtg aacttgatag tctgggtaga agaggatacc atctccattt aggtcaatgc
     2161 tattgtcaag ctgttgagca tggtaagtcg cgtcattagc tttttcaaaa agacgaacag
     2221 cagcatagag gggataaatc cccaaatcca tgagggctcc accagcaaaa cggtctgaaa
     2281 agacatttgg tgtttgtcca gccaacaagt caggcatctt ggaagagtat ttggcatagt
     2341 tgaaatctgc tcctaacact tgcttatctg ctaaaaagtt tttgatagta gtaaaggctt
     2401 tctcgtggta attacgagct gcttcaaaga taaaacagtt atttttttca gctgtttgaa
     2461 tcaaatcaaa ccattcttgt ggttgagtga cagctggctt ttcgagaata acatgtttac
     2521 cagcagacaa ggcagctttt gcctgagcaa aatgtaagga gtttggactg gcgatataga
     2581 ctaaatcaaa agaagatttg aagaagactt ctaattgatc gaatagttgg atattctgat
     2641 agcgagaagc aaaggttgct gcagtttcta gttttctaga atagattgcg accagttggt
     2701 attctccact ggtatgggct gcttctatga aatgatggct gatagcgcca gttccgatga
     2761 cacctaattt tagcataaat actccttttc cgattttaaa tccttctttc attataacat
     2821 agatagacgg gactatccaa cagagaggag aaaatttcaa ataagctatt agctttcttt
     2881 tccgaataaa tagatagaag catagaatct agcaaaccta gatttaaaaa tgtgctataa
     2941 tagaaggagg aaaaggagga ttctcagaca tccaggtatc agcccaacta atgatttgtc
     3001 aatttatccg cgatatgctg gacttgccag caaaaaatgt gacgattttg gagggaagta
     3061 acattcacgt cttgccttcc atgccctact cagcgcaaga tttctatact agtatagacg
     3121 tcttggcgga gttagataat ggaatccaag ttatcatcga aattcaggtt catcatcaga
     3181 attttttcat caatcgccta tgggcttatc tgtgcagtca ggttaatcaa aacctagaaa
     3241 aaattcgcca acgtgaaggt gatacccacc agagctacaa acaaatcgca ccagtatacg
     3301 ctatcgcaat tgtcgatagt aattacttct cagatgacct agcttttcat agttttatag
     3361 taaaatgaaa tgagaacagg acaaatcgat caggacaatc aaatcgattt ctaacaatgt
     3421 tttagaagta taggtctact attctagctt caatctacta gaaattccat agatataaaa
     3481 ctacataatc tctacagata cggatgttgg agttgatgta agatgctttg gcttgctaga
     3541 ggaattgtgg attgccaaat tgtatcattg aaattattgc tcaaatttgt tatgatataa
     3601 atatgaataa aagtagacta ggacgtggca gacacgggaa aacgagacat gtattattgg
     3661 ctttgattgg tattttagca atttctattt gcctattagg cggatttatt gcttttaaga
     3721 tctaccagca aaaaagtttt gagcaaaaga ttgaatcgct caaaaaagag aaagatgatc
     3781 aattgagtga gggaaatcag aaggagcatt ttcgtcaggg gcaagccgaa gtgattgcct
     3841 attatcctct ccaaggggag aaagtgattt cctctgttag ggagttgata aatcaagatg
     3901 ttaaggacaa gctagaaagt aaggacaatc ttgttttcta ctatacagag caagaagagt
     3961 caggtttaaa gggagtcgtt aatcgtaatg tgaccaaaca aatctatgat ttagttgctt
     4021 ttaaggttga agagactgaa aagaccagtc taggaaaggt tcacttaaca gaagatggcc
     4081 aaccttttac acttgaccaa ctgttttcag atgctagtaa ggctaaggaa cagctgataa
     4141 aagagttgac ctccttcata gaggataaaa aaatagagca agaccagagt gagcagattg
     4201 taaaaaactt ctctgaccaa gacttgtctg catggaattt tgattacaag gatagtcaga
     4261 ttatccttta tccaagtcct gtggttgaaa atttagaaga gatagccttg ccagtatctg
     4321 ctttctttga tgttatccaa tcttcgtact tactcgaaaa agatgcggcc ttgtaccaat
     4381 cttactttga taagaaatat caaaaagttg tcgctctaac ctttgatgat ggtccaaatc
     4441 cagcaacgac cccgcaggta ttagagaccc tagctaaata tgatattaaa gcgactttct
     4501 ttgtgcttgg gaaaaatgtt tctgggaatg aggacttggt gaagaggata aaatctgaag
     4561 gtcatgttgt tggaaaccat agttggagcc atccgattct ctcgcaactc tctcttgatg
     4621 aagctaaaaa gcagattact gatactgagg atgtgctaac taaagtgctg ggttctagtt
     4681 ctaaactcat gcgtccacct tatggtgcta ttacagatga tattcgcaat agcttggatt
     4741 tgagctttat catgtgggat gtggatagtc tggactggaa gagtaaaaat gaagcatcta
     4801 ttttgacaga aattcagcat caagtagcta acggctctat cgttttgatg catgatattc
     4861 acagtccgac agtcaatgcc ttgccaaggg tcattgagta tttgaaaaat caaggttata
     4921 cctttgtgac cataccagag atgctcaata ctcgcctaaa agctcatgag ctgtactata
     4981 gtcgtgatga ataagcaaga aaaaataggt ctgttagata tttgacagac ttatttttta
     5041 cagaagatag tactacttaa aaaatgtttt atgctataat tgatgaataa aatagaagga
     5101 gaagcatatg aatacctatc aattaaataa tggagtagaa attccagtat tgggatttgg
     5161 aacttttaag gctaaggatg gagaagaagc ctatcgtgca gtgttagaag ccttgaaggc
     5221 tggttatcgt catattgata cggcggcgat ttatcagaat gaagaaagtg ttggtcaagc
     5281 aatcaaagat agcggagttc cacgtgaaga aatgttcgta actaccaagc tttggaatag
     5341 tcagcaaacc tatgagcaaa ctcgtcaagc tttggaaaaa tctatagaaa aactgggctt
     5401 ggattatttg gatttgtatt tgattcattg gccgaaccca aaaccgctca gagaaaatga
     5461 cgcatggaaa actcgcaatg cggaagtttg gagagcgatg gaagacctct atcaagaagg
     5521 gaaaatccgt gctatcggcg ttagcaattt tcttccccat catttggatg ccttgcttga
     5581 aactgcaact atcgttcctg cggtcaatca agttcgcttg gcgccaggtg tgtatcaaga
     5641 tcaagtcgta gcttactgtc gtgaaaaggg aattttattg gaagcttggg ggccttttgg
     5701 tcagggagaa ctatttgata gcaagcaagt gcaagaaatc gcagcaaatc acggaaaatc
     5761 gattgctcag atagccttgg cctggagctt ggcagaagga tttttaccac ttccaaaatc
     5821 tgtcacaacc tctcgtattc aagctaatct tgattgcttt ggaattgaac tgagtcatga
     5881 ggagagagaa aacttaaaaa cgattgctgt tcaatcgggt gctccacgag ttgatgatgt
     5941 ggatttctag aaaatcataa aaagaattgt acattattct aatttttgat ataatagtca
     6001 gcaggaaaga aagtcttatg gcgttcttca agcgagcttg ggatagtggg agccaagtag
     6061 ggcaaaataa tgagctggcg ctttctgtag tattttcaaa aacaatgaag taataaatta
     6121 gggtggaacc gcgtttctga cgcccctagg ttaaatcaac ctaggattgt cggacgcggt
     6181 tcttttgctt attcagtcta ttgtgtgaaa gaaaggagag ccgtggacaa cctttatctt
     6241 gtaaaagacg atagtcaact agctacattt cgtgattttg tagtaagaaa tactgaaaag
     6301 ttgaaagatt atcaatcttt tttaaagaat gaacttgcag tctgtgattt accgcaagcc
     6361 gttatttggt cagattttaa tgctgctaca cagattatta gggaaagcgc tgttccagcc
     6421 tatacaaata atagacgaat ggttatggcg cctgatttag ctgtttggaa agagttgtat
     6481 ttgtatcaat tgatggacta cgagtgttct cagcaaactc aagcaataga aagtcactat
     6541 cattctttat ctgaaaattt cctcttacag attgtaggac atgagttagc tcattggtcg
     6601 gagcattttt tagatgattt tgatggttat gactcttata tctggttcga agaggggatg
     6661 gttgaatata ttagtcgcaa gtatttcttg acagaagagg aatttcaagc ggaaaaaatt
     6721 tgtaatcaat ctctcgtaga actttttcag aagaagtatg gttggcattc attgaatgat
     6781 tttggttctt cgacttatga taagaactat gcaagtattt tttatgaata ctggcgcagc
     6841 tttttgacag tagataagtt ggtagaaaat ttaggtagtg tacaagcggt cttagattct
     6901 tatcatttat gggcaaatac agaaaaaact tttcccttgt tagattggtt tgttcagcag
     6961 aaattaattg aaaaagaaat ataaaaacta aaggagtaaa caatgtctaa gaaattgaca
     7021 tttcactgcg tcagtggcag aaacctcctt acagtcggac tgccccacgc tcagcactag
     7081 agtgcctgag ctagacgcag tactaactcg tcttgcctcg tatgatcgac gaggcagact
     7141 catgtcgcaa gtaatttata taaaaaggag taaacaatgt ctaagaaatt gactttccag
     7201 gaaatcatcc ttactttgca acaattttgg aatgaccaag gttgtatgct tatgcaggct
     7261 tatgataatg aaaaaggtgc gggaacaatg agtccttaca ctttccttcg tgctatcgga
     7321 cctgagccat ggaatgcagc ttatgtagag ccatcacgtc gtcctgctga cggtcgttat
     7381 ggggaaaacc ctaaccgtct ctaccaacac caccaattcc aggtggtcat gaagccttct
     7441 ccatcaaata tccaagaact ttaccttgaa tctttggaaa aattgggaat caatcctttg
     7501 gagcacgata ttcgttttgt tgaggacaac tgggaaaacc catcaactgg ttcagctggt
     7561 cttggttggg aagtttggct tgacggaatg gaaatcactc agttcactta tttccaacaa
     7621 gtcggtggat tggcaactgg ccctgtgact gcggaagtta cctatggttt ggagcgcttg
     7681 gcttcttaca ttcaagaagt agactctgtc tatgatatcg agtgggctga tggtgtaaaa
     7741 tacggagaaa tctttatcca gcctgagtat gagcactcaa aatattcatt tgaaatttcg
     7801 gaccaagaaa tgttgcttga aaactttgat aagtttgaaa aagaagctgg tcgtgcatta
     7861 gaagaaggct tggtacaccc tgcctatgac tatgttctca aatgttcaca tacctttaat
     7921 ctgcttgacg cgcgtggtgc cgtatctgta acagagcgtg caggctatat cgctcgtatc
     7981 cgtaacttgg cccgtgtcgt agccaaaacc tttgtcgcag aacgcaaacg cctaggctac
     8041 ccacttttgg atgaagaaac aagagctaaa ctcctagcag aagacgcaga ataaagagag
     8101 tgacaaatta cgaaaatggg cgaacagagt gagccctgag ccagttgccg cagtgatgaa
     8161 ggtatcctta gtgaaactaa ggatactagg caaaattgga gacttttggc tccaatttta
     8221 gcaatgaaac aacgaagttg gttgcttgcg tgccaatcac ataaggcaaa ctggaaaata
     8281 aaaagatact tttcggagaa aaaacatgac aaaaaactta ttagtagaac tcggtcttga
     8341 agaattacca gcctatgttg tcacaccaag tgaaaaacaa ctaggcgaaa aaatggcagc
     8401 cttcctcaag gaaaaccgcc tgtcttttga agccattcaa accttctcaa caccacgtcg
     8461 tttggctgtt cgtgtgactg gtttatcaga caaacagtct gatttgacag aagatttcaa
     8521 gggaccagca aagaaaattg ccttggatag tgatggaaac ttcaccaaag cagctcaagg
     8581 atttgtccgt gggaaaggtt tgactgttga agatatcgaa ttccgtgaaa ttaagggtga
     8641 agaatatgtc tatgtcacta aggaagaagt tggccaatca gttgaagcca ttgttcctgg
     8701 tgttgtagat gtcttgaagt cattgacttt ccctgtcagc atgcactggg ctggaaatag
     8761 ctttgaatac atccgccctg ttcacacttt aactgttctc ttggatgagc aagagtttga
     8821 cttggatttc cttgatatca agggaagtcg tgtgagccgt ggccatcgtt tcttggggaa
     8881 agaaaccaaa attcagtcag cattgagcta tgaagaagac cttcgtaagc agtttgtaat
     8941 cgcagatcca tgtgaacgtg agcaaatgat tgttgaccaa atcaaggaaa ttgaggcaaa
     9001 acatggtgta cgtatcgaaa ttgatgcgga tttgctgaat gaagtcttga atttggttga
     9061 atacccaact gccttcatgg gaagttttga tgctaaatac cttgaagttc cagaagaagt
     9121 cttggtgact tctatgaagg aacaccagcg ttactttgtt gttcgtgatc aagatggaaa
     9181 actcttgcca aacttcattt ctgttcgtaa cggaaacgca gagcgtttga aaaatgtcat
     9241 caaaggaaat gaaaaagtct tagtagcccg cttggaagac ggagaattct tctggcgtga
     9301 agaccaaaaa ttggtgattt cagatcttgt tgaaaaatta aacaatgtca ccttccatga
     9361 gaagattggt tcccttcgtg aacacatgat tcgtacgggt caaatcactg tacttttggc
     9421 agaaaaagct ggtttgtcag tggatgaaac agttgacctt gctcgtgcag cagccattta
     9481 caagtttgac ttgttgacag gtatggttgg tgaatttgac gaactccaag gaattatggg
     9541 tgaaaaatac acccttcttg ctggtgaaac tccagcggtg gcagctgcta ttcgtgaaca
     9601 ctacatgcct acatcagctg aaggagaact tccagagagc aaggtcggcg cagttctagc
     9661 cattgcagac aaattggata cgattttgag tttcttctca gtaggattga ttccatcagg
     9721 ttctaatgac ccttatgccc ttcgtcgtgc aactcaaggt gtggttcgta tcttggatgc
     9781 ctttggttgg cacattgcta tggatgagct gattgatagc ctttatgcat tgaaatttga
     9841 cagtttgact tatgaaaata aagcagaggt tatggacttt atcaaggctc gtgttgataa
     9901 gatgatgggc tctactccaa aagatatcaa ggaagcagtt cttgcaggtt caaactttgt
     9961 tgtggcagat atgttggaag cagcaagtgc tctcgtagaa gtaagcaagg aagaagattt
    10021 taaaccatct gttgaatcac tttctcgtgc ctttaacctg gctgagaagg cagaaggggt
    10081 tgctacagtt gattcagcac tatttgagaa tgaccaagaa aaagctttgg cagaagcagt
    10141 agaaacactc gttttatcag gacctgcaag tcagcaattg aaacaactct ttgcgcttag
    10201 cccagtcatt gatgctttct ttgaaaatac tatggtaatg gctgaagatc aggctgtccg
    10261 tcaaaatcgt ttggcaatct tgtcacaact aaccaagaaa gcagctaagt ttgcttgttt
    10321 taaccaaatt aacactaaat aaaatttgat aaacggactt tatcttatta caaaggagaa
    10381 gaaatggatc cgaaaaaaat tgctcgtatc aatgagcttg ctaaaaagaa aaaaacagaa
    10441 ggcttaacac cagaagaaaa agtggaacaa gccaaactac gtgaggagta catcgaaggt
    10501 tatcgccgcg ctgttcgtca ccacattgaa ggaatcaaaa ttgtggacga agaaggaaac
    10561 gatgttacac cagaaaaact acgccaagta caacgtgaaa aaggattaca tggccgtagt
    10621 cttgatgatc caaattcata ataatactct tcgaaaatca aattcaaacc acgtcagctt
    10681 caccttgccg tacttaagta cagcctgcgg ctagcttcct agtttgctct ttgattttca
    10741 ttgagtatat gtattctttc ttttaacaaa gatagatgaa acgataacaa agagactagc
    10801 agtttgtgtt tgctagtctt ttttcgctaa aaaaggaacc ataatggttc ctaaaaacta
    10861 tcattagtaa cttgcaccgg ctgtagcgtc tgcgtcacca ccgtggcctc cagcatcccc
    10921 tgaatcagaa gcgccagaag tagcatcggc gtctccatga cctccggcag caggagcaaa
    10981 tggtccgcta ccacccacca aacgttgacc agtctctttt aggtaccagt caagccatgg
    11041 ttggaagtta aagacgattt cattgatacc agcgtatgat ccatcaggat agtacattgc
    11101 ttggtagttg tgagtgttga taacacctgc aggagaacct ggaacgatcg tacggacgta
    11161 ttcttggttt ccgttgcgaa gtgttccgat aacccactct acgttcttca tacgtgctgg
    11221 tggaagagaa ccatgaacag tcgacatacg gctacctgat tgaggtggta cacgtttagc
    11281 gaacatagtg tctggatctt ggtgagcgtt gttgtagtag aggaattggt tgttgtcgtc
    11341 agcgtatgtc aattcaaatg gcatagcttt caagaacata tcaatttggt taactgttag
    11401 gataccgtgg tccaatttga cataggtatc accagaaaca gcaccagtga atgctgcaac
    11461 tttttctacc cattctggat cgtcagggtc aacttctgtg atggttgtag cgattggttt
    11521 tccacaatcc aagtcttctg attcgattgg ttttggtttt ttcaatttcg aaacgactcc
    11581 tacgtattta acaaagttat ctaagcaagt ttcaaggaat ttaacagtgc cttcgttggt
    11641 gatatttccg ttgttatcaa aagcttcctt agctttacca agaaggaatt cgttacctgg
    11701 aagcgtgtag gcattaacac ctggagcatc aaggatttta cgaaggtgaa cttgagcacg
    11761 tgatgttcct tggtcatagt atgatgcacc cacaatcata acaggcttgt tttcaaatgg
    11821 atgaacttcg tatgaaagcc attcaagtac agatttgagt gaagctgaga tagtgtggtt
    11881 atgctcagga gtagcaatga taacaccatc tgcacgagta attttgttat ataaataacg
    11941 taattggaaa ctttcatccc atttttcatc ttggttaaac attggaactt cgtcaatttc
    12001 aagaacttct aattcaaatt tgagtttgaa gtagcgacgg ataaattcca agagcttacg
    12061 gttatatgat tgatcgtagt ttgatccaac aagtccaaca aatttcattc tttttggtct
    12121 cctatcttac aaattttccc agtcaaagtc ttcagcatct ttgcgaagta attcttgtgc
    12181 attacgtaat ttttctgtga tttttacaaa gatacggaag tcatcaaaga tggcatccaa
    12241 tttcttgata acatcaaggt caaccaagtc gccacttggg ttaaatgctt gaagagagtg
    12301 tgagagcaag aattcatctg gaagaacatt tgccttgatt tcaggagcat tcaagatttg
    12361 acgaagttgc aattgggcac gagatgaacc aagcgtaccg taagaagcac ctgtaatcat
    12421 gattggtttg ttcaaaagtg ggtaaatacc ataagataac caagcaagag cgctcatcaa
    12481 aacagctgga atagagtgat catactcagg agtaccgata ataacgccat ctgcctcttc
    12541 gattttagca gcaatttcca atatttcagc aggtacttgc ttgtcagctg gtttgttgaa
    12601 gacaggaatg gccttgattt caacaagttc aatttcagct ttgtcagtaa agtgtttttg
    12661 catgtattga agcaattgac ggtttgtaga acgttttgaa tttgttccaa caatagcaat
    12721 aagttttaac atgagatttc ctttctcttt ttacataata caattttaaa attccattga
    12781 aacagttgtc tctatagagt aggaattcct gaagaacagc ttaggtggcc ttctttatcg
    12841 atgaggatga cttcgatgcc ctccaaactt tcgacttgcc agaggataga agcaggtctt
    12901 tctccaaaga gtcgagtcgt ccagatttcg ccatcgactg atttatcaga gatgattgtt
    12961 agactcgcta gttccgtttc aacaggatat cctgtttgac tgtcaaaaat gtgatggtaa
    13021 tcttgtccat cgacggtcag gtgacgttca taaatgcctg aagtcacgac agatttattg
    13081 acaacaggga tggtcattaa atgatttccc ctaggattgg ctgggtcttg aatcccgatt
    13141 tgccatgggt tatcccctct tgcctgattt tttccaatgg tcaggatatt ccctcccaga
    13201 ttgatcaagg cagaagtcac cccctctttc ctaagaaatt gggcaacctt atccgcactg
    13261 tatcctttgg ctaaacaacc tagatcgatc ttcattcctt tctgttttaa aaacacagta
    13321 gaagtagaag aatctaactc gataccatga ggattgatta gaggcagcac cgattcaatt
    13381 tcttgaggct gggcgacctt ggcatctgaa aaaccgatac gccaggtttg aattaaggga
    13441 ccaatgctga tattgaggtg gctagagagc gctaggctat gctctaaccc aagtgaaatc
    13501 agctcaaaca ggtctggatg aaccgtgacg ggggctattc ctgcttgata attgatttcc
    13561 atcaactcag attcttgact attggcgttg aagcggtatt caagttcttt gagcaagtca
    13621 aaggattttt ggagaaagat atcggcttgc tcatccacta atgaaatagt gatagtagtc
    13681 cccattagcc gttcagaatg tgaacgaaga gtcaagctac caactccttt ctcttataga
    13741 aaataagttg taatatcaaa taatcatcta aattgaagcc cttacatttc attttcatgt
    13801 tattataata ccataaagtt agaattttca caaacaaaat ttggaaaaag tcaagaaata
    13861 tgctcataaa attcatcagg cttgaaaaca ggataaatgg ggaattattt ttgataaaaa
    13921 atgctgaaat aatagtaccc cccttgtaaa cgctaacggt aaatggtata ctagtaaggt
    13981 aaatttagaa tgaaggcagg aaatttttat gagtaaaatc gttgtagtcg gtgctaacca
    14041 cgctggtaca gcatgtatca ataccatgtt ggataatttt ggaaatgaga acgaaattgt
    14101 tgtatttgac caaaactcta acatctcttt cctaggatgt ggaatggctc tttggattgg
    14161 tgaacaaatt gacggtgctg aaggcttgtt ctattctgat aaagaaaaat tggaagctaa
    14221 aggtgctaaa gtttacatga actcacctgt tctttcaatc gactatgata acaaagtagt
    14281 tacagcggaa gttgaaggaa aagagcacaa agaatcatac gaaaaattga ttttcgctac
    14341 aggttctaca ccaatcttgc caccaatcga aggtgttgaa attgttaaag gaaaccgcga
    14401 atttaaagca actcttgaaa acgtacaatt cgtgaaattg taccaaaatg ctgaagaagt
    14461 tatcaataaa ctttctgaca agagccaaca cctcgaccgt atcgccgttg ttggtggtgg
    14521 ttacatcggt gttgaacttg ctgaagcctt tgaacgtctt ggaaaagaag ttgtccttgt
    14581 tgatatcgtt gatactgtct tgaacggtta ctatgacaaa gacttcacac aaatgatggc
    14641 gaagaacttg gaagatcaca acatccgctt ggctctaggt caaactgtta aagcaatcga
    14701 aggtgacggt aaagttgaac gcttgattac tgacaaagaa agctttgacg tggatatggt
    14761 tatccttgca gttggtttcc gtccaaacac agcccttgca ggtggtaaga tcgaactctt
    14821 ccgcaacggt gccttccttg tagacaagaa acaagaaaca tctatcccag acgtttacgc
    14881 tgttggtgac tgtgcgactg tttatgacaa tgctcgtaaa gatacaagct atatcgctct
    14941 tgcttcaaat gctgttcgca ctggtatcgt tggtgcctac aatgcttgtg gacatgaatt
    15001 ggaaggaatc ggtgttcaag gttcaaatgg tatctcaatc tacggtcttc acatggtttc
    15061 aactggtttg actcttgaaa aagcgaaagc tgctggttac aacgcaactg aaacaggctt
    15121 taacgatctt caaaaaccag aattcatgaa acatgacaac catgaagtag caattaagat
    15181 tgtctttgac aaagatagcc gtgaaattct tggtgcccaa atggtttccc atgatattgc
    15241 aattagcatg ggaatccaca tgttctcact tgctatccaa gagcatgtga caattgataa
    15301 attggcattg acagacctct tcttcttgcc acacttcaac aaaccataca actacatcac
    15361 aatggctgcc cttacggctg aaaaataaaa atgaatgagc tatctggcct taagttaagg
    15421 tcagatagtt tttagctaat ctgtccccat acaattatag tttttttatc ttgtgcttca
    15481 ttctgttctg acttaaaatg aaaaggtagc taccaataca aatgatgagg ataaaacaaa
    15541 tgactgaaaa tcgttatgaa ctaaataaaa acttggcaca gatgctcaag ggtggtgtta
    15601 ttatggatgt gcagaatcct gaacaggctc gtatcgcaga agctgctggt gcggcagctg
    15661 tgatggcctt ggaacgaatt ccggctgata ttcgtgcagc tggaggagtt tcccgcatga
    15721 gcgacccaaa gatgattaag gaaatccaag aagcggttag tattccagta atggctaagg
    15781 tcagaatcgg gcattttgtt gaagctcaga ttttagaggc tattgaaatt gattatatcg
    15841 acgagagtga agttctatct ccagctgatg accgtttcca tgtggacaag aaagaattcc
    15901 aagttccttt tgtctgtggt gctaaggatt tgggtgaagc cttgcgtcgt atcgctgaag
    15961 gtgcttccat gattcgtacc aaaggagaac cagggacagg ggatatcgtc caagctgttc
    16021 gtcatatgcg tatgatgaat caggaaattc gccgcattca aaacttacgt gaggacgagc
    16081 tttatgttgc tgccaaggat ttgcaagtcc ctgtagaatt ggtccaatat gttcatgaac
    16141 atggaaaatt gccagttgta aatttcgctg ctggaggtgt tgcaacgcca gcagatgctg
    16201 cgttaatgat gcaactaggg gcagaggggg tctttgtcgg ttcaggtatt ttcaagtcag
    16261 gagatcctgt taaacgagcg agtgccattg ttaaggctgt gactaacttc cgtaatcctc
    16321 aaatcctagc tcaaatctct gaagatttag gagaagccat ggttggtatt aatgaaaatg
    16381 aaatccaaat tctcatggct gaacgaggaa aatagatgaa aatcggaata ttggccttgc
    16441 aaggggcctt tgcagaacat gcaaaagtgc tagatcaatt aggtgtcgag agtgtagaac
    16501 tcagaaatct agatgatttt cagcaagatc agagtgactt gtcgggtttg attttgcctg
    16561 gtggtgagtc tacaaccatg ggcaagctct tacgtgacca gaacatgcta cttcccatcc
    16621 gagaagccat tctatctggc ttaccagtgt ttgggacctg tgcgggctta attttgctgg
    16681 ctaaggaaat cacttctcag aaagagagtc atctaggaac tatggatatg gtggtcgagc
    16741 gtaatgctta tgggcgccaa ttaggaagtt tctacacgga agcagaatgt aagggagttg
    16801 gcaagattcc aatgaccttt atccgtggtc caattatcag cagtgttgga gaagatgtag
    16861 aaattctagc aacagttgat aatcaaatcg ttgcagccca agaaaaaaat atgttggtaa
    16921 cttcttttca tccagaattg acagatgatg ctcacttaca ccagtacttt atcgaaatgt
    16981 gtaaagaaaa aagacagtaa aaaagctcaa aaaatcactt cctgtgctat actgtaattg
    17041 aaattttttt agtttttgcg ttagaactgt ttcctcagtc ctagcgcctt ttctttttga
    17101 taagtctgct tggtggagag tagtttgtaa acgattctga gaatcttatg agcacaagca
    17161 atgacggctt tcatcttgct tcctctttgg gaaattcgat tgtaaaactc taagcaggca
    17221 gagcctgctt agagttttac atatgctact atctttgtta tagctaactt aaattctcat
    17281 ttattaagta tacctaagtt ttatgttta
//
LOCUS       DAVKMU010000040        17021 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKMU010000040 DAVKMU010000000
VERSION     DAVKMU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.040"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            314..1360
                     /gene="pheS"
                     /locus_tag="VW343_001563"
     CDS             314..1360
                     /gene="pheS"
                     /locus_tag="VW343_001563"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9756093.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1360..1869
                     /locus_tag="VW343_001564"
     CDS             1360..1869
                     /locus_tag="VW343_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9756094.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1946..4351
                     /locus_tag="VW343_001565"
     CDS             1946..4351
                     /locus_tag="VW343_001565"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9756095.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNESAADALSVSIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLAETDLVITVADKPVALAGVMGGQATEISEKS
                     SRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4419..5420)
                     /locus_tag="VW343_001566"
     CDS             complement(4419..5420)
                     /locus_tag="VW343_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9756096.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTDSGIAPVTVIVKKSYDFYTKAETFHTTRFGTIVLHSRKQDIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5576..6065
                     /locus_tag="VW343_001567"
                     /pseudo
     CDS             5576..6065
                     /locus_tag="VW343_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6149..6415
                     /locus_tag="VW343_001568"
     CDS             6149..6415
                     /locus_tag="VW343_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9756097.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            6676..8925
                     /gene="metE"
                     /locus_tag="VW343_001569"
     CDS             6676..8925
                     /gene="metE"
                     /locus_tag="VW343_001569"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9756098.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLSAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFTTERVGENAELRARIAG
                     LTDADYTRLPVFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8989..9855
                     /gene="metF"
                     /locus_tag="VW343_001570"
     CDS             8989..9855
                     /gene="metF"
                     /locus_tag="VW343_001570"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9756099.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELVPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10540..12753
                     /gene="pnp"
                     /locus_tag="VW343_001571"
     CDS             10540..12753
                     /gene="pnp"
                     /locus_tag="VW343_001571"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9756100.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12769..13386
                     /gene="cysE"
                     /locus_tag="VW343_001572"
     CDS             12769..13386
                     /gene="cysE"
                     /locus_tag="VW343_001572"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9756101.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13398..14282
                     /locus_tag="VW343_001573"
     CDS             13398..14282
                     /locus_tag="VW343_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9756102.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14369..15712
                     /locus_tag="VW343_001574"
     CDS             14369..15712
                     /locus_tag="VW343_001574"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9756103.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15705..16091
                     /locus_tag="VW343_001575"
     CDS             15705..16091
                     /locus_tag="VW343_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9756104.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16095..16979
                     /locus_tag="VW343_001576"
     CDS             16095..16979
                     /locus_tag="VW343_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9756105.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         5052 a         3329 c         3896 g         4744 t
ORIGIN      
        1 gctataaagt tagataagaa taagctaaca agcagaagta agataggtct tgcaagaatg
       61 gtaaaaatct gataaactaa gtagtaattg aataggaatc tgaaagacta gtagcccaag
      121 ggaagtatat cagggaggag agccgtgact gcaagctctc tatatgaaag ctgggtgaat
      181 tcacttgcgc atggatttag aaatgaaggt ctgacttgtc agatgaaaac ggatggtacc
      241 gcgtgtcaac gctccgagtg gagtttttgg catgtggttt tctttttatc tacgagagac
      301 tgatggagga aatatgtcaa ctattgaaga acaattaaaa gcgcttcgcg aagaaacgct
      361 ggctagcttg aagcagatta ctgctggaaa tgaaaaagag atgcaagatt tgcgtgtctc
      421 tgtccttggt aaaaagggtt cgctcactga aatcctcaaa gggatgaaag atgtttctgc
      481 tgagatgcgt ccaatcatcg ggaaacacgt caatgaagct cgtgatgtct tgactgctgc
      541 ttttgaagaa acagctaagc tcttggaaga aaagaaagtc gcggctcaac tggctagcga
      601 gagtatcgat gtgacgcttc caggtcgtcc agttgcgact ggtcaccgtc acgttttgac
      661 acaaaccagt gaagaaatcg aagatatctt catcggtatg ggttatcaag tcgtggatgg
      721 ttttgaagtg gagcaagact actataactt tgaacgtatg aaccttccaa aagaccaccc
      781 agctcgtgat atgcaggata ctttctatat cactgaagaa atcttgctcc gtacccacac
      841 gtctccagtt caggcgcgtg ctatggatgc ccatgatttt tctaaaggtc ctttgaagat
      901 gatctcgcca gggcgtgtct tccgtcgcga tacggacgat gcgacccaca gtcaccaatt
      961 ccaccaaatc gaaggcttgg tagttgggaa aaatatctct atggctgatc ttcaaggaac
     1021 gcttcagttg attgtccaaa aaatgtttgg tgaagagcgt caaattcgtt tgcgtccatc
     1081 ttacttccca ttcacagagc catctgttga ggtggatgtt tcttgcttca agtgtggtgg
     1141 agaaggctgt aatgtatgta agaaaacagg ttggatcgaa attatggggg ccggtatggt
     1201 tcacccacgt gtccttgaaa tgagtggtat cgatgcgact gtatactctg gctttgcctt
     1261 tggtcttgga caagagcgtg tagctatgct ccgttatgga atcaacgata tccgtggatt
     1321 ctaccaagga gatgtccgct tctcagaaca gtttaaataa tgattagaaa agtagaaatg
     1381 gcagatgttg aggtgttggc taaaattgcc aaacaagcct ttcgtgaaac ctttgcgtat
     1441 gataatacgg aagagcagtt acaggaatac tttgaagagg cttatagtct gaaaactttg
     1501 tcaactgagt tgggaaatcc tgactctgaa acctatttca ttatgcatga ggaggagata
     1561 gctggttttc tcaaagtcaa ctggggaagt gctcaaactg agagagaatt agaggacgct
     1621 tttgaaattc aacgcctcta tgtgctacaa aaattccaag gatttggact aggtaagcaa
     1681 ctgtttgaat tcgcacttga acttgctaca aaaaatagtt tttcttgggc ttggttaggt
     1741 gtttgggagc ataatacaaa agctcaagcg ttttataatc gatatggttt tgaaaaattt
     1801 agccaacatc attttatggt tggtcaaaaa gtagatacgg attggttact gagaaagaaa
     1861 ttaaggtaag aagaggattc ttctattgaa atgataggaa aggaaaagaa ctagagtgac
     1921 aactgtcatt ctggagaact aaattatgct tgtatcttat aaatggttaa aagaattggt
     1981 ggacattgat gtgccatcac aagagttggc tgaaaaaatg tcaactacag gaatcgaggt
     2041 agagggtgtc gaatcaccag ctgctggtct ctcaaaaatt gtcgtcggtg aggtcttgtc
     2101 ttgcgaagat gtgccagaga ctcacctcca tgtttgtcag gttaacgttg gcgaagaaga
     2161 agagcgtcag atcgtttgtg gtgccccaaa tgtgcgtgct gggatcaagg tcatggtggc
     2221 tcttccagga gctcgtatcg ctgataacta caaaatcaaa aaaggaaaaa tccgtggttt
     2281 ggagtcactt ggaatgatct gttcacttgg tgaattggga atttctgact cagttgtgcc
     2341 taaggaattc gcagatggca tccaaatctt gccagaaaat gccgtgccag gtgaggaagt
     2401 cttttcttac ctagacttgg atgatgaaat catcgaactt tccatcacac caaaccgtgc
     2461 agatgccctt tctatgtgtg gagtggctca cgaagtggca gccatctatg acaaggcagt
     2521 caactttaaa gaatttactc taacagaaac taatgaatct gcggcagatg ccctttctgt
     2581 cagcattggg acagacaagg cgccttacta tgcagctcgt atcttggaca atgtgactat
     2641 cgcaccaagt ccacaatggt tgcaaaacct tctcatgaac gaaggcatcc gtcccatcaa
     2701 taacgttgta gacgtgacaa actacatcct gctctacttt ggtcaaccta tgcatgcttt
     2761 tgacttggac acatttgaag ggactgacat ccgtgtgcgt gaagcgcgtg ctggtgaaaa
     2821 gttggtgacc ttggacggtg aagaacgaga cttggctgag acagacctcg tgattacagt
     2881 tgctgacaaa ccagtagccc ttgccggtgt tatgggtggt caggctacag aaatttctga
     2941 aaaatctagt cgtgttatcc ttgaagctgc tgtttttaat ggcaaatcta tccgtaagac
     3001 aagtggtcgc ctgaaccttc gttctgagtc atcttctcgc tttgaaaaag gaattaatgt
     3061 ggcaacagtt aatgaagccc ttgatgcggc agctagcctg attgcggaac ttgcaggtgc
     3121 gacggtgcgt aagggtatcg tttcagcggg tgagcttgat acttcagatg tagaagtttc
     3181 ttcaactctt gctgatgtta accgtgtcct cggaactgag ctgtcttatg ctgatgtcga
     3241 agacgtcttc cgtcgtcttg gctttggtct ttctggaaat gcagacagct ttacagtcag
     3301 cgtaccacgt cgtcgttggg atatcacaat cgaagctgat ctctttgaag aaatcgctcg
     3361 tatctatgga tatgaccgtt tgccaaccag ccttccaaaa gacgatggta cagctggtga
     3421 attgactgtg acacaaaaac tccgccgtca agttcgtacc attgctgaag gagcaggttt
     3481 gacagaaatc atcacctata ctctaacaac tcctgaaaaa gcagttgagt ttacggctca
     3541 accaagtaac cttacggaac tcatgtggcc aatgactgtg gatcgttcag tcctccgtca
     3601 aaatatgatt tcaggtatcc ttgataccgt tgcctacaac gtggctcgta agaataaaaa
     3661 cttggccctt tacgagattg gaaaagtctt tgaacaaaca ggtaatccaa aagaagaact
     3721 tccaaatgaa atcaacagtt ttgcctttgc cttgacaggc ttggttgctg aaaaagattt
     3781 ccaaacagca gcagttccag ttgatttctt ctatgctaag ggaatccttg aagcgctctt
     3841 tactcgcttg ggactagaag tgacctatac agcaacagct gagattgcca gccttcaccc
     3901 aggacgtaca gcagtcattt cactcggtga ccaagttctt ggttttcttg gccaagtgca
     3961 tccagtcact gccaaggctt acgatattcc agaaacgtat gtagctgagc ttaacctttc
     4021 agccatcgaa ggggcgctcc aacctgctgt tccatttgtg gaaatcacga aattcccagc
     4081 agtcagccgt gacgttgccc ttctcctcaa ggcagaagta actcaccaag aagttgtaga
     4141 tgctatccaa gctgccggcg tgaaacgttt gacagatatc aaactctttg acgtcttctc
     4201 aggtgagaaa ttgggacttg gtatgaagtc catggcttat agcttgacct tccaaaatcc
     4261 agaagatagc ttaacggacg aagaagtcgc acgctatatg gaaaaaatcc aagcatcgct
     4321 cgaagaaaaa gtgaatgcag aagtgcgtta aacgatagta ggatataaat tccccctcta
     4381 gtttatgaac actagagggg tgattttata taaaataatt aaaatgtgat ttctgtaaaa
     4441 atacatctat gatcagagtt ttgaaaactt acaatgtcta aatctttaac atagtagtgg
     4501 tttttaggca ataaaatatg atctatcgtt gcattaaaaa gttttggact ttggctattc
     4561 caagttcctc tttcaaaagg tggcagtgca tttaatgcgt ccctatgaga gcttattttt
     4621 gcaagtgctc catgacgcat agttgcatta aaatcacctg caataatagc ctttggatat
     4681 tttgaagcca attgattatg aatgatgttt aagtcttgct tccagatttc cattaaacct
     4741 ggcagaggag gagcagtatg caaagcaatg atatctggta tatcttgttt tctcgaatgt
     4801 aatacaattg tcccgaaccg tgttgtatga aaagtttcag cttttgtata gaaatcgtaa
     4861 cttttcttga caatcacagt cacaggagct attccactgt cagtaggtgg agaagtaaaa
     4921 atatcatagt tggccataga aagtccaact tgatgaaaca atagtttgat tctctggttt
     4981 tcttgctcac ctctgatatt ggtagctagt tcagggaata tagccatatc ggcgtcaaaa
     5041 tggctaaaaa ttcgctcaat atgttgtgct tctatttgat tagcgacgtt ccaagttact
     5101 aattttaagg ttttagtctt atttgatgcg gaagaaaggg ttttatctgt tccgaataat
     5161 agtaatatga gcgataataa gagcaaaatg ccagagtaaa accgtatttg tttatttttc
     5221 ttgtaaaaag atagggaaaa aaatacgaaa cttaaaatgg cagccatagt tgcagtagct
     5281 aaggggaagg cataaatctg tgccaagata ggtctggtag atagccaaat attaagatta
     5341 gaagatggaa aaaaagaata agttatgata gggataacaa agagaatgac cgaaagggca
     5401 gagagaaatg ttttaatcat tgtgtgagta cctcaatttg caatggttgc tcggagctaa
     5461 tgtgactttg tgactttact tgattgatat tctttttctt gtcttcctta tagattaagt
     5521 acaacccaag taaaaaaagt ccaatttaag attgaaatga ttaaaaggca cagagatgca
     5581 agacaattat actacaaaag gtaaacattt gacaatcgat agccgtcgct taatcgaaag
     5641 atggaaaaaa gaaggaaaat caaatagaga agttgcctct ctacttggaa aagctcctca
     5701 aactatccac actgaaatca agcgtgggac agtccgaaaa tgtcttggaa aagggcgctt
     5761 caaagaggtt tattctgccg actacgctca acagtcttat gaaaacaatc gcaagcgctc
     5821 ggtcaagaaa tcaagcttga ccaaggaact aaaggaaaag attctccact atacataacc
     5881 aaaaattttt gcctgaaatg atggtgatgg ctaaaggggt taacgtggga atttcaacca
     5941 tttactattg gattcatcat ggaaagttgg ggttaagcaa acaggatttg ctttatccta
     6001 gaaaaggaaa atctgttaag aaacaagtta gtcccaattt taaacccgct gatcaatcta
     6061 tcgaatcata attcgctcat ttttttaaaa atacatagac gtaaaaaaaa gagggttaag
     6121 tgatgaagaa tagtgctatt gggagtaatt ggaaggatgt ccatctgagc tctttagcaa
     6181 ggaggaaatc cttgaaagtg atatgcgagt ggctatcatg agcgagttga ttgaggctag
     6241 gtatgaacaa ggaatcagtc agaaaaagct ggaagaagtc agtggaataa gccagcctgt
     6301 catagctagg atggagacag gaaagactag tcctcagttg gatacagtct taaaagtcct
     6361 agccagttta ggaaagacac tagcaatcgt cccacttgaa cagggaaaaa gttgactaga
     6421 atcagttata gtcttttcta ataacaagcc atagtcactt ataagaatta ctaataacgc
     6481 gtttgagcat tctaactaaa atacagctat aaaacaagct atacaaagga tttgagacgt
     6541 ttgcttcaag tctttttctt ttgtccgaaa cagatatata gtttcaaact atatcaaacc
     6601 ctaatttttt acaattatac agacgctttc ttttctgtta agatagtttc aacaacaaat
     6661 tttggaggac acatcatgtc aactacaatc gtcggtttcc ctcgtttggg cgaattccgt
     6721 gaattaaaat ttacaactga aaaatacttt agaaaagaaa tctcagagga agaactttta
     6781 tcagcagcaa aagacttgcg tgctaaacac tggaacattg tcaaagaaaa aggcatcact
     6841 gaaattccat caaatgactt ttctcactat gataacttcc tagatgcggc tttccttttc
     6901 aacgtggtgc ctgcatccgt tcaaaacttg gacttgtctg accttgagcg ctacttcgct
     6961 ttgggacgtg gttaccaggg agaaaaaggg gacgttcgcg cccttccaat gaagaaatgg
     7021 ttcaacacta actaccacta catcgttcct aaatttgaaa aagacactca agtaaaattg
     7081 gctggtcaca agattttcga tgagttccaa gaagccaaag aacttggatt gaacactcgt
     7141 cctgttcttg taggtccctt cactttcctt caattgtcag actttgaaga aggcgtgaaa
     7201 gcagacgact tcgtagatag cttagtggct gcttaccaag aagtttttgc taaattggct
     7261 gaacttggtg cgactcgcat ccaattggat gaagcggctc ttgtaaaaga cttgacagct
     7321 gaagaaaaag ctctcttctt gaacctttac aacaaactct tggctgacaa aaaaggtctt
     7381 gaagtcttgc ttcaaactta tttcggtgat gttcgtgacg tatacgctga ccttgtaaac
     7441 ttaccagtag atgctatcgg tcttgacttt gttgaaggta agaaaactct tgaactcgtt
     7501 aaaggtggct tcccagctga caagactctc tatgtaggta ttgtcaatgg taaaaacatc
     7561 tggcgcaaca actacgaaaa gagcttggct gttcttgagc aaatcccagc tgaaaacatc
     7621 gttttgacaa gctcttgctc acttcttcat gtgccattta caactgctaa tgaagaattt
     7681 gaaccagcac tcttgaacca ctttgccttt gcagttgaaa aattggatga aatccgtgac
     7741 ttggatgcta tccgcaatgg tcaaggttca gaagctcttg cagccaacaa agaactcttt
     7801 acgactgagc gtgttggtga aaatgctgaa cttcgtgcgc gtatcgctgg cttgacagac
     7861 gcagactaca ctcgtttgcc agtctttgca gaacgtgaag ctatccaaga agaagctttc
     7921 aaacttccag ctcttccaac aacaacgatt ggttcattcc ctcaaacaaa agaagttcgt
     7981 gccaaacgtt tggcttaccg taaaggtgaa ttgtctcaaa aagagtacga tgctttcctt
     8041 gctgaaacga ttgacgaatg gatcaagtgg caagaagata ttgactttga tgtccttgtt
     8101 cacggtgaat ttgagcgtaa tgacatggtt gagtacttcg gtcaaaactt gtcaggatac
     8161 ctcttctcta aaaatggttg ggtacagtca tacggtatgc gtggggtgaa accaccaatc
     8221 atctggggtg atgtcactcg tcttaaccct atcactgtta aatggtctag ctatgcacaa
     8281 agccgtacaa acaaacctgt taaaggtatg ttgactggac ctgttaccat cctcaactgg
     8341 tcattcccac gtgaagacat ctctatcaag gattcaactc ttcaaatcgc ccttgctatc
     8401 aaggatgaag tgcttgacct tgaagctgct ggtgtgaaaa tcatccaaat tgatgaggct
     8461 gctcttcgtg aaaaattgcc actccgccgt agcgactggt acgaagacta ccttgactgg
     8521 gctattcctg ccttccgctt ggtacactca acagtagcgc cagacacaca aatccacact
     8581 cacatgtgtt actcagaatt tacagatatc atcccagcta tcgataacat ggatgcagac
     8641 gttatttcct ttgaagctag ccgttcaaac cttgaaatct tggacgaact caaagcgaaa
     8701 aacttccaaa cagaagtggg acctggggtt tacgatatcc actcacctcg tgtaccaaat
     8761 gaaggcgaaa tcgacaacac aatcgaagcc atccttgcta aagtgccaag caagaaagtt
     8821 tggatcaacc ctgactgtgg tttgaaaaca cgtggtatcc cagaaacaaa agaaagcttg
     8881 atccgccttg ttgaagcagc taaagctgcg cgtgagaaat tgtaagattg gatttctctc
     8941 catacagtgg ttgttcaaaa agaaatcaac tagaaaaggt tattacatat gtcacgccaa
     9001 acaccgtcac tctcatttga agtgttccct ccaaacccag ccgtgggtaa tgataacatt
     9061 atttcagcct tgcaggatat gcaggagttg gttccccatt ttatcagtgt aactgccagc
     9121 aataataaat ttaatatcaa ggaaacgacg gttcgtttgg ctgactttat ccaaaatgat
     9181 ttggcgattc cgaccattgc ccacttgcca gctatttatc taactaagga caaggttgct
     9241 gaaactattg ctgacttgga caaagttggg gtgcagaaaa tcttggctct tcgtggggat
     9301 attattccag acgtggaacc acaaaaggat ttccgctatg caaccgactt gattgagttt
     9361 atcaaggaac gagcccctca ctttgatatt attggagctt gctatccaga agggcatcca
     9421 gattcgccaa atcagatttc agatattcaa aatcttaaga agaaagtgga tgcaggctgt
     9481 tcgagcctcg taactcagct cttctttgac aatgagcgct tctatgattt tcaggacaag
     9541 tgtatcttgg ctgggattga tgttcccatt catgcaggaa ttatgccaat tctgaatcga
     9601 aatcaggctc tccgactctt gaagacttgt gagaatatcc atcttccacg caaatttaaa
     9661 gccatcttag acaagtatga gcatgaccct gagtcgctca gagcagcagg acttgcctat
     9721 gcagtggacc aaatcgtgga cttggtaact caggatgttg ccggtgtgca tctctatacg
     9781 atgaataatg ctgatacagc aaaatacatc catcaagcaa cccatgcctt gtttaatcac
     9841 cagtctctag gataataaaa gcaaaccatt cttctcaggt gaggggaatg gttccttttt
     9901 aatggtaaag acctcacttt ttaagaaaaa tatgataaaa taaattctgt acgtacttga
     9961 tacaaagatg aaggtattaa ataatacaat gcatttaaac tgtaagattt aacgacacga
    10021 gtgagtcaaa tcgatgttat ttgacgagag agttagtaaa gcatttagct aatcgcccga
    10081 tagcgattag cgtgaaagct ttagatactt tagtattaaa cctttctaat gatgtatctt
    10141 gaaaactgaa cttgcctaca actctgtgga aaaagataaa tctccctagg agtagtcgct
    10201 ccgtcgttcg atttcctatc ttcctttgag ttgcttgacg gcttggtatc ttgatgactt
    10261 gaattttggt ctcatttctt aggaaaaaag ataagcttcc tagcactcgt cgagtgtgac
    10321 gtcaccttcc tatttttcta cagaaatgtt cggcaagccg aaccgtccaa aatatcttgt
    10381 gtaattgaac acggccgaaa agctgtgtaa aaaagataaa ctgtcttgtc ttcatcgaag
    10441 acttcgtcag tttcctattt ttactttgct tttgacgtcc ttggtatctt gatctttgta
    10501 ggcaaggcgt ataatttcat caatccaaag gggattaaaa tggcaaaaca agtgtttcaa
    10561 acgacttttg cgggtcgtga gttaattgta gaaactggtc aggttgctaa gcaagcaaat
    10621 ggctctgttg tcgtacgtta cggtgagtca actgtcttga ctgctgccgt tatgtctaag
    10681 aaaatggcaa ctggggattt cttcccactc caagtcaact acgaagaaaa aatgtatgcg
    10741 gctgggaagt ttcctggtgg ctttatgaaa cgtgaaggac gtccttcaac agatgcgacc
    10801 ttgacagcgc gtttgattga ccgtccgatt cgtcctatgt ttgcggaagg tttccgtaat
    10861 gaagtccaag tcatcaatac agtgctttct tatgatgaaa atgcatctgc accaatggct
    10921 gctatgtttg gttcatcttt ggcactgtct atttcagata ttccatttga cggaccaatt
    10981 gctggggtac aagtgggata tgtagatggc caaatcatca tcaacccaag tcaagaacaa
    11041 gcagagcaat ctcttcttga attgacagta gctggaacca agcacgctat caacatggta
    11101 gagtctggtg ccaaagaatt gtcagaagaa atcatgttgg aagcgcttct taaagggcac
    11161 gaagctgtca aagaattgat tgccttccaa gaagaaatcg ttgctgctgt cggtaaagaa
    11221 aaagcagaag tggaattgct tcacgtggat gctgaattgc aagctgaaat cattgcagcc
    11281 tacaacagtg acctccaaaa ggcagttcaa gtagaagaga aattggcccg tgaagctgca
    11341 actcaagcag tgaaagacca agtgactgcc gtttacgaag aaaaatatgc gaaccacgaa
    11401 gaatttgacc gtattatgcg tgatgtggct gaaatcttgg aacaaatgga acacgcagaa
    11461 gtgcgacgtt taattacaga agacaaggtg cgtcctgatg gtcgtaaggt cgatgaaatc
    11521 cgtcctttgg atgcggttgt tgacttcctt cctcgtgtac atggttcagg tctctttact
    11581 cgtgggcaaa ctcaagctct ttcagtcttg accttggctc cgatgggaga aactcaaatc
    11641 attgatggtt tggatccaga gtacaagaaa cgctttatgc accactataa cttccctcaa
    11701 tattctgtag gggaaacagg tcgttacggt gcgccaggtc gtcgtgaaat cggtcacggt
    11761 gcccttggtg agcgtgctct tgctcaagtc ttgccaagct tggaagaatt cccatacgct
    11821 atccgtctag tagcagaagt tttggaatca aacggttctt catctcaagc ttctatctgt
    11881 gcgggaactc ttgcccttat gactggtggt gtgccaatca aggcgccagt agctggtatt
    11941 gctatgggac ttatctcaga tggaaataac tacacagtat tgacagatat ccaaggtttg
    12001 gaagatcact ttggagatat ggacttcaag gttgcaggta ctcgtgatgg gattacagcc
    12061 cttcaaatgg atatcaagat tcaagggatt actgcagaaa tcttgacgga ggctcttgct
    12121 caagccaaga aagcgcgttt tgaaatcctt gatgtcattg aagcaaccat tccagaagtt
    12181 cgtccagaat tggctccaac tgctccgaaa attgatacga tcaagattga tgtggacaag
    12241 attaagattg tcatcggtaa gggtggagaa accatcgaca agattatcgc tgaaacaggt
    12301 gttaagattg atatagacga agaaggaaat gtgtctatct actctagtga ccaagatgct
    12361 attaaccgtg ccaaagaaat tattgctggt ttggttcgtg aagccaaagt ggatgaagtt
    12421 taccgtgcta aagtcgttcg tatcgagaaa tttggtgcct ttgttaacct ctttgataag
    12481 acagatgccc ttgttcatat ctctgagatg gcttggactc gtaccaatcg tgtagaggat
    12541 ttggtagaaa tcggggatga agttgatgtt aaggttatca agattgatga aaaaggccgt
    12601 atcgatgcct ctatgaaggc tcttctacct cgtccgccaa aacctgagca tgatgaaaaa
    12661 ggtgaaaagt ctgagcgccc tcaccgccca cgtcatcaca aggatcacaa acctaagaaa
    12721 gaatttacag aaacaccaaa agattcagag taagaaaagg agaaatgtat ggggtggtgg
    12781 cacgaaacca ttgatatcgt aaaagaaaat gatccagcgg cccgcaccac tttggaggtt
    12841 ttgctgactt atccaggtgt caaggccttg gcggcccacc gtctctcgca ttttctctgg
    12901 aagtacgact tcaaactctt agctcgtatg cacagtcagt tttggcgctt ttggactcag
    12961 attgagattc atccaggagc ccagattgat tcaggtgtct ttattgacca tggttctggt
    13021 ctggtgattg gagagacagc gattgttgaa aaaggcgttc ttctctatca cggagtgact
    13081 ctcgggggaa cagggaaaga ctgtggcaaa cgccatccga ctgtacgaaa gggagccctc
    13141 atatcagccc atgcccaagt tatcgggcct gtggaaattg gtgaaaatgc caaggtcggt
    13201 gctgcagcag ttgtcgtagc agatgtacct agtgatgtga cggttgtcgg tattccggcc
    13261 aagattgtcc gtcttcatgg taagaaagat gagcctgtta ttcacgaagt cgaagaaaaa
    13321 cgggagtatt atgtcaataa actcgagcag gctaaagatg ccagtcacag atcgtctggt
    13381 ttgtagagga tacctatatg attcaagcaa gaaacaagtt aagccaagag gagctatctg
    13441 aggcgaaaaa agtaattaac tgttgccaaa actatgacgg tacctatcgc gatccctatc
    13501 tctctaacat gcttaatttt gacccaaaca tgcccgcctt tttcctttat tatgaaaaag
    13561 gcaaacttgt tggtttatta actgtctatg cagatgacca agatgtagaa gtgacgatat
    13621 tggttcatcc aggtcatcgc cgtcagggga ttgcgcgtgc attgtttact agttttgaga
    13681 gagaaacagc ttctttcccc attcggtcag tcacttttca gacagaacgt atttttttag
    13741 agaatcatcc tgactttgcc agtaactggg gactaatcga ggatgaagag acagaaactt
    13801 ggttaggtaa ggatagaaga ccttatccgt tagcaaatgt ttcccatctt gaagttttgt
    13861 tagcagatag ttcgtatcag gatcaaatta gtcaattaaa atttcaggca ttttcagagg
    13921 aacatgaatc gagagaagtt gtggatagat atgtcgctga agctctgaag gatccagaaa
    13981 gtcgcttata tattttgtta aaagacggtc aggttattgg gacttgcacg gtagatttat
    14041 cgactaatac gaattacttc tacggtttag caatatcgga acctgaacgt gggaaaggct
    14101 atggaagcta cttagcaaaa tccctcgtca accaactaat tgagcaaaat gacaaggaat
    14161 ttcagattgc agtggaagat agcaatgtag gtgccaaacg tttgtatgaa aaaattggct
    14221 ttgtcaaaca gacacaggtg gtttatctga atgagaaagg agcaagggat tccgaagtgt
    14281 agagacattc ggactgaaat ttacttgagc tcttagttta gtgatgaaac taacagttct
    14341 tggattttag ctttcctgat tatgatttat gattaaaatc tatgacacca tgtctcgtga
    14401 tttgcgagaa tttgtcccga ttgaggacgg caagatcaag atgtatgttt gtgggccaac
    14461 cgtgtacaac tatattcacg tgggaaacgc ccgttcgacg gtagcttttg atacgattcg
    14521 tcgctatttt gagtaccgtg ggtataaggt tgcctatatt tccaatttta cagatgtgga
    14581 tgataagatt atcaaccgtg ccagggaaga aggcatcaca cctcaggagg ttgcggataa
    14641 gtacatcgct gcctttcgtg aggatgtgac ggccttaggc gtgaaacctg cgactcgcca
    14701 tccgcgtgta gtggagttta tggcagacat catccgtttt gtggaagact tgatcgagaa
    14761 aggctttgcc tatgagagtc aaggggatgt ctatttccgt gtagaaaaat cccacaacta
    14821 tgctaaattg gctaataaaa ccttggaaga tttggagcta ggtgcttcag gtcgtaccga
    14881 tgaagaaacg gctcgtaagg aaaatcctgt agattttgct ctatggaaat cttccaaacc
    14941 aggtgagatt tcttgggata gtccttgggg acctggacgt ccgggttggc atattgagtg
    15001 ttcagtcatg tcgacagaga ttttaggtga taccattgat atccacggtg gtggagctga
    15061 cctagagttt ccacaccaca ccaatgaaat tgcccaatct gaagctaaaa caggcaaggc
    15121 ttttgctaac tactggatgc acaatggctt tgtcaatatc gacaatgtca aaatgtctaa
    15181 gtccttgggt aacttcatta cggtacacga tgcccttaaa actcttgatg ggcaagtact
    15241 tcgtttcttc tttgcgaccc agcactaccg taagcctatc aacttcacgg aaaaggcagt
    15301 tcgcgatgcc gagaccaatc tcaagtatct gaagaatact tacgaacaac catttacagg
    15361 aaacgtggat gcccaagagt tacaaaactt taaagataag tttgtagcag ctatggatga
    15421 agattttaat gctgccaacg gtatcacagt tgtctttgaa atggccaaat ggatcaactc
    15481 agggaactat gatgcaagtg tcaagcaagc tcttgcagat atgttagaaa tttttggaat
    15541 tgtctttgtt gaggaagttt tggatgcaga gattgaagac ttgattcaaa aacgccaaga
    15601 ggcgcgtgcc aatcgagact ttgagacagc agaccaaatc cgtgaccaat tggttactca
    15661 aggaattaag ctccttgata ccaaggatgg agtgaggtgg acacgtgatt gatgtcaatc
    15721 tcattaacgg gattgcgcta gcctttgagg gggatgcggt gtattctatg tatattcgcc
    15781 gtcacctcat cctcaaaggt atgaccaaac ccaataaact ccatcaagaa gcaaccaagt
    15841 atgtgtcagc caaggctcag gctcgcctga ttgctctcat gttggaggag caggtcctaa
    15901 cggaaaaaga agaagaaatc tacaaacgtg gccgcaatac caatagccac acaaaggcta
    15961 aaaatgcaga tgtcgtgact tatcgtatgt ccacgggatt tgaagcggtt atgggctatc
    16021 tccatatgac tgagaatctg gaacgtcttg agagtttggt ttcatggtgc atccaaaaag
    16081 tggagggcta gaacatgagg gaaaaagaac tacaagactg gtttcctgag gctcggattt
    16141 cagaccaacc agtagagaaa gagggctatc tcacgctccc tttagcttct cagcagtgga
    16201 ttttgctgga ggaagctggg ctcagcgagc gtgaaaagca gttggttgcc cttttgaccc
    16261 agcaggagca ggctcgttcg ctaaaccctt ggtattccta tctggttgaa ggcaagggac
    16321 aggcaccgca agtttttaaa aagattcagt tggtttattg ccatctttct tattttcagc
    16381 aggaaaatct ggcttcttgg ctagatatga tgcggactct ttttccgaat tgtcagacag
    16441 tgctacaggt cggagctcag gattatgttt tcgtgcttca acaagacaaa tacacttctg
    16501 taagagatat tttaagtgat acgattgaag cggttgagta tgactttgga cttcgtcttt
    16561 ctatcatgtt gggtcaggtt tggtctcaga caggacatca agccctatca gacttaatca
    16621 aagctgagcg ggatttgttc aagacatggt ggcgtcaggg tcaccaaggt gttcatactt
    16681 tttctcagct ctatctttgg agtatgggag aaagactcgt ggacttgaag ccaattaaag
    16741 aatgtctaca ccagatgatt ttggatcaag atcagattca ggaaatcatt ctctctcttt
    16801 gggaaaatag tgctgttctc actaaaacag cccagcaact ctatctgcac cgcaattctc
    16861 tccaatacaa gattgataaa tgggaagagt tgacagggct tcagttgaaa gagttgaccg
    16921 acctgacctt gtgttatcaa ttgattttag gttctttgtc aactatagtt ggtttgtaaa
    16981 gaagttaata tttggagaag aggattgcca tcttctccat t
//
LOCUS       DAVKMU010000041        16664 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKMU010000041 DAVKMU010000000
VERSION     DAVKMU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.041"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..996
                     /locus_tag="VW343_001577"
                     /pseudo
     CDS             1..996
                     /locus_tag="VW343_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1184..2746)
                     /gene="guaA"
                     /locus_tag="VW343_001578"
     CDS             complement(1184..2746)
                     /gene="guaA"
                     /locus_tag="VW343_001578"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9756106.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2888..3586
                     /locus_tag="VW343_001579"
     CDS             2888..3586
                     /locus_tag="VW343_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9756107.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERHVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3629..4525)
                     /locus_tag="VW343_001580"
     CDS             complement(3629..4525)
                     /locus_tag="VW343_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9756108.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQTDIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4534..5469)
                     /locus_tag="VW343_001581"
     CDS             complement(4534..5469)
                     /locus_tag="VW343_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9756109.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5466..6191)
                     /locus_tag="VW343_001582"
     CDS             complement(5466..6191)
                     /locus_tag="VW343_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9756110.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(6275..6910)
                     /locus_tag="VW343_001583"
     CDS             complement(6275..6910)
                     /locus_tag="VW343_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9756111.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(6912..7739)
                     /locus_tag="VW343_001584"
     CDS             complement(6912..7739)
                     /locus_tag="VW343_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9756112.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKVRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDIIQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7761..8096)
                     /locus_tag="VW343_001585"
     CDS             complement(7761..8096)
                     /locus_tag="VW343_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEU9756113.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(8097..8813)
                     /locus_tag="VW343_001586"
     CDS             complement(8097..8813)
                     /locus_tag="VW343_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU9756114.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWFKQADLAIG
                     DFEETINKDHYLAGYPLFNAPAEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNGIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKMKAEIERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHL
                     YVVEPSETIEKDGDKKLIVY"
     gene            complement(8956..9147)
                     /locus_tag="VW343_001587"
     CDS             complement(8956..9147)
                     /locus_tag="VW343_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756115.1"
                     /translation="MKEILICDIIKRKGLHKKVGRMKMQRRQNRNQGGLAFRFMKGLV
                     NFLGVIASGAIRDLWRYSC"
     gene            9336..9947
                     /locus_tag="VW343_001588"
     CDS             9336..9947
                     /locus_tag="VW343_001588"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9756116.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9992..10720
                     /gene="rlmB"
                     /locus_tag="VW343_001589"
     CDS             9992..10720
                     /gene="rlmB"
                     /locus_tag="VW343_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9756117.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKNLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENSLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(10759..11670)
                     /gene="trxB"
                     /locus_tag="VW343_001590"
     CDS             complement(10759..11670)
                     /gene="trxB"
                     /locus_tag="VW343_001590"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9756118.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVRKIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(11736..11960)
                     /locus_tag="VW343_001591"
     CDS             complement(11736..11960)
                     /locus_tag="VW343_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9756119.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(12038..12781)
                     /locus_tag="VW343_001592"
     CDS             complement(12038..12781)
                     /locus_tag="VW343_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9756120.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(12781..13581)
                     /locus_tag="VW343_001593"
     CDS             complement(12781..13581)
                     /locus_tag="VW343_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9756121.1"
                     /translation="MNVTTILASDWYQNLMQLIPNGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(13739..14095)
                     /locus_tag="VW343_001594"
     CDS             complement(13739..14095)
                     /locus_tag="VW343_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9756122.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(14089..14619)
                     /locus_tag="VW343_001595"
     CDS             complement(14089..14619)
                     /locus_tag="VW343_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9756123.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(14619..15113)
                     /locus_tag="VW343_001596"
     CDS             complement(14619..15113)
                     /locus_tag="VW343_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9756124.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            15248..15694
                     /locus_tag="VW343_001597"
     CDS             15248..15694
                     /locus_tag="VW343_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9756125.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            15691..16338
                     /locus_tag="VW343_001598"
     CDS             15691..16338
                     /locus_tag="VW343_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9756126.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILTASVLQYIAIVYYM"
BASE COUNT         5020 a         3757 c         2946 g         4941 t
ORIGIN      
        1 tatcatcaac caaaaaattg ctcaaaagtt aattgaaaaa atttctatga ctgatattgc
       61 ccatcagctt tccatctcaa cttcaactgt tattcgtaag ctcaatgatt ttcactttaa
      121 acatgatttt tcgcgtcttc ctgagattat gtcttgggat gagtatgctt ttacaaaggg
      181 aaagatgagc ttcattgcgc aagattttga aaagctcgat atcatcactg ttcttgaggg
      241 tagaacacaa gctatcattc gaaatcactt tcttcgctac gatagagttg tccgatgtcg
      301 cgtcaaaatt attactatgg atatgtttag cccttactat gacttagcta gacagcttcg
      361 ctttcaaatt tctaggctca ggctgaaaca gtctcccaga ctgtttcact cccgaatgct
      421 aaaatcgttc ttgatcgctt tcacattgtg caacatctta gccgtgctat gagtcgtgtg
      481 catgttcaaa tcatgaatca gtttcatcga aaatcccatg aatacaaggc tatcaagcgc
      541 tactggaagc tcatacaaca ggatagccgt aaactgagtg ataagcgttt ttatcgtcct
      601 acttttcgta tgcatttaac caataaagag attttagaca agcttttgag ctattcggaa
      661 gacttgaaac accactatca gctctatcag ctcttgcttt ttcacttcca gaataaggaa
      721 ccagagaaat ttttcggact tattgaggac aatctgaagc aggttcatcc tatttttcag
      781 actgtcttta aaaccttcct caaagataaa gaaaagatta tcaacgccct tcaactaccc
      841 tattcaaacg ccaaattgga agcgaccaat aatctcatca aacttatcaa gcacaatgcc
      901 tttggttttc ggaactttga aaacttcaaa aaacggattt ttatcgctct gaatatcaaa
      961 aaagaaagga cgaaatttgt cctttctcga tcttagcttt tcttcaaccc actacagttg
     1021 acaaagagcc tgaaaactac ggtctaattt tttgtccaaa tgtaaaccac ttagaactat
     1081 tattcaattt attcttttta gcaaatgttc ccgttttaat cccagttaca gtttttaatc
     1141 ctttgtaacc gttgatttta caaagctttt aatttttttg cgattactcc cactcaactg
     1201 ttgcaggtgg tttacttgta atatcgtaga cgatacggtt aacatgatcc acttcattta
     1261 cgatacgtac tgagattttt tgaagtactt cccatggaat tttggcaaaa tcagcagtca
     1321 taccatcgat agaagtgata gcacggattg caatcgtgta gtcatacgta cgaccgtcac
     1381 ccataacacc gactgaacga acgcctgtgt taacagtgaa gtattgccaa atatcgcggt
     1441 caagtccagc tttagcgatt tcttcacgaa gaatagcgtc tgattcacga acggtttcaa
     1501 gtttctcttc agtgatttca cccatgacac ggatagcaag tcctggtcct gggaatggtt
     1561 ggcgccatac gatatggtct ggcataccaa gctctgtacc aagagcacga acttcatcct
     1621 tgtaaagagt attgagtggt tcaatcaatt caaactgcat atcttctgga agaccaccca
     1681 cgttgtggtg tgacttgata gtttgagctg tatccgtacc agactcgata acatctgtat
     1741 ataaagtacc ttgagcaagg aatttcacat ctttgagctt gcttgcttcg tcatcgaata
     1801 catagacaaa ctcgttaccg atgattttac gtttttgttc agggtcagaa acgccagcaa
     1861 gtttgtcaag gaaacgttta gcagcgtctg ctttgacgat attcaaacca aacttaccac
     1921 cgagcatgtc cataacttga tcagcttcgc ctttacgaag aagaccgtgg tccacgaaga
     1981 tacagatcaa ttgatcgcca atcgcttttt ggagaagaac cccaacgaca gatgagtcaa
     2041 caccacctga tagaccaaga aggacacgtt tatcaccgac ggtttcacga atttttttga
     2101 tctgcatgtc aatgaaatta tccattgacc agtcaccttt agccttacaa atgttaaggg
     2161 caaagttacg aaggatatca tttccgtata cagaatgacg aacttctggg tggaattgga
     2221 taccgtaaat gtgtttatct gggttttcga tggctgcgta tgggcagtca gctgatgtac
     2281 ctgtacgaac aaagtcagca ggaatctcag taaccgcatc accatggctc atcaaaacag
     2341 tctgttcatc aggtgttgat tcaaaaagcg ctgatggtgt gtgagttagg gttgattgac
     2401 cgtattcacg atttccagca tcacctgcag gaacaacttt tcctccaagt ttatgggtca
     2461 ataactgcat accataacag attcccaaaa ttggaattcc gagttcgaag atttctgggt
     2521 caatatcaaa tgaaccatct tcatatacag aatttggacc acctgataga ataattccta
     2581 caggattgac ttcacgaact tcagcagctg aaattttatg gctttttagt tctgaaaaaa
     2641 caccaatctc acggatacgg cgtgaaatca gctggttgta ctggctacca tagtccaata
     2701 cgatgatttt ttctacatct tgcaaatcag ttgaaatgtt gctcatcttt ttcctttctc
     2761 aaaagaaata tcttttctca atattctatc acaaataagg gcgaattacc aactaaacaa
     2821 ggcgaagttt gactttttct agtttattgg ggtacaatag agaaaagata gaaatgaagt
     2881 gaaaaatatg ctacctgctt atatgaaaat ccatgatcag ataaaaaagg atattgacga
     2941 gcaccgttgg gctattggtg agaggcttcc cagtgaaaga gatttagctg agcagtttgc
     3001 ggtcagtcgc atgaccctcc gccaagccgt atctctatta gtcgaagaag gcgtcttaga
     3061 gcgccatgta ggaagcggca cctttgtttc cagtactcga gtacaagaaa agatgcgagg
     3121 gacaaccagt tttactgaaa ttgtcaaatc ccaaggtaaa gttccctcta gccagctcat
     3181 ttcctacaaa aaaaccattc ccaatgagca agaagttgcc aagctaggaa tttttccaac
     3241 ggacaatatt atccgaatgg agcgggtccg ctatgccgac caagttcccc tagtttatga
     3301 agttgcttct attcctgaaa aattcattaa ggactttaaa aaagaagaaa tcaccagtca
     3361 tttcttccaa accttgcaaa aacatggcta tcgtatcggc aaatctcaac agaccatcta
     3421 tgctcgcctt gctaaagaaa agattgccca ctatttggaa gttgaaaaag gacatgctat
     3481 tcttggattg acccaggttt cctacctaga agatggtact gcttttgaat acgtaaaaag
     3541 tcaatatgta ggcgaacgct ttgaatttta tcttgaaaat aattagatta ccacgcaaaa
     3601 actctctgtc acggacaaag agtctttttt atttttctaa gaataaatct ttcaaagaaa
     3661 tcaaagttac agcaaacaat atcattgcca taccaagaaa atcaatggga taaaattgtt
     3721 cattcattat taagaaggca aagaaaatcg ccgatattgg ctcaattgaa gccaacaagc
     3781 ttgacttgac cggtcctatc agactggctc ctttaaggaa agctgtatag gcaaagacag
     3841 tcccgataag gataatgcct gcaaacgcaa ggagaaaatc aagactagtc gggatatcgg
     3901 tctgtagaac ccctgtaaaa ggaagggcga ccaaacctgc tatgaccatt cccacaccaa
     3961 tgaccaagct gctcccccac tttttaatca aggctatggg taaaatgata tacagagcat
     4021 aagtcaaggc agaaaagaga ccccagaaca gaccagcagg tgtcatggat aactggtcca
     4081 actgcccatg tgttgcgatc aggaaggttc ctccgatggc gaatatgatg gaaactatct
     4141 ctcccagtgt cggtgccacc ctatccttga tacagctata aattaaaatt ccgacaggac
     4201 aaacatactg aagcaccgtc gctgttcccg cattggtctc ctgaatagca gacagatagg
     4261 cgaattggtt gagaaaaaga ccaatcagag caaaaataag aagagacagc aaactctttc
     4321 tatcctttaa aaagaccagt attttatcct ttgcagtagc ataagccaag agcatgagaa
     4381 ttccaccagc gattaaaaga cgcaagttgg tcaagaccag agccgaaatt ccgtgtgcca
     4441 ttaggtattg gccactcgtt cctgacaacc cccaagcaat accagccaca actgttagta
     4501 aagtcccttt taaactattt gacatgtctc tccttactct tctttgcgaa tcaagttcat
     4561 cacttgattg cggtctaata tccattgagc tctctcgtcc ttttcatagg tacgattcga
     4621 taggaaaata gtggcttctt gcttctgacg attccacatg ataaaggtac ctgtatagcc
     4681 cgtatggtct agccaatctc cttccaaatt ccatgctaaa gaacgttcct tgtcatccaa
     4741 aggagaaaaa ttttgactta agtctcttgc aaaatcatct gctaaatagt gttctaaaaa
     4801 gatttgtaaa tcctttatag tcgaaaataa accagcgctc ccagcatgtc tacccaggag
     4861 acgagccttg ggatcatgca ctatgcctgc ctctacacct ctaactgttg gaacagcaag
     4921 ctcaactggc ccaaacttgg tttccgtcat tccccaaggt ttccagactt gatcctttaa
     4981 aatcacatcc aaatcttgat taaaaattct ttccaaaata aagcccaaca gcaaaaaatg
     5041 gacatccgaa taaagaaagg ctggctgact tcgtctgttg agatgaaaca tcgcttcctt
     5101 taattcaggg gctgttaaaa gatcacgatt aggaataaaa ggatcaaggt ctgttgcatg
     5161 agtcaagagc tggcgaatag tgatgtctgg ataatcactc tcaggtaaaa aatctattac
     5221 cagtctatca atatctaatt gacctatttc ccacaagaag gtacaaactg tgccaacccc
     5281 aacaaccttg ctgacactag ctaggtcata aactagtcct gcctcagtct gcaagccatg
     5341 ctctgggtca ctctggccta aatagaactc tgtccattga ttgtccttaa aatacgcaaa
     5401 agaggctccg ggataaattc ctgcctcgat ttgttcttct atttttttaa taatcttggt
     5461 ccacttcata cttcttcaaa ccacaattca acattatttc tatctttaca aaggaagaat
     5521 ttttcagact taggaataaa atattcagta gactcaaact tctggcgaag acttgctatg
     5581 tctaattcat tgaccaagaa cttgagcata gataagtccc aggtaaccgt attgtccaca
     5641 gtcaaatcct gcccctgagc tggaataaaa tcaagggatg ccccaatttc agatgattcc
     5701 aagaaacttt cgatatcagt tgggagatgt aattccatag aaatctcaaa tttacttaaa
     5761 gaaattgatg ccaaatctgt ttgaaattca ggcttttctc ctacttctac tagacttgct
     5821 ctgtcatctt ccgcatgaat caaaatcaaa tcatcttctg gtgagaaaat ttcaaaagcg
     5881 tagccatttt gacctttata taatcgatga atcgaatctg ttttagataa gattccttca
     5941 atttctaagg gattttccac cttgacaatc aatctagcta gtttttttct tccctctacc
     6001 ttacgagtac gcatactggg agcttcttct aaaaccagct tttcaagacc cgtttggtca
     6061 cctagtgaca gaaaggccga ttcttctaac aaagccttca ttccaagggt ttcaatataa
     6121 aatgtttcat ttaattttct attattagct tttaaagtag gaataatccg tacaatctga
     6181 tttacattca taaattcctc cgtcactttt tattttaaag gattttagaa ttttttacaa
     6241 gatattatgc tcaaatttaa cattatttag ctatttagta aagatagtct ttcaaggatt
     6301 ttgacaaagc ctgataacca gcaatactga ggtgcagtcc atcagttgta tattcttttt
     6361 tgagttggcc tgcttggtct gtcaaacaat caaatactgg cacaaattcc acctgcatat
     6421 aggcagatgc aagctcttga taggcttgat tccagttctg aattttttca ttcgagcgga
     6481 tatagactgc ctgctggtac tcctctccct cattgacagg caaaatggaa agcaatttaa
     6541 tctctgtcaa tggataatcg cgagcaacgg attgaatgat agcttcgaga ttattgagag
     6601 cctcattcac aggaacatcc tttccgatat catttgtccc aatcagaaga aaaattttat
     6661 ctactgctcc accatataga tgagcatcaa gattctctag taacagtcct gtctgatagc
     6721 cacgaattcc tcgattgaca atcgtctttg aagtcccaaa tagctcctgt agaggataat
     6781 actcgacaat ggaatcccca ataaaaagaa tgtttggttc tacaacagaa atgtgattta
     6841 ggtgacgata cttagtttga attttttctt gttcctttag gagccaattt tctaataact
     6901 gtactgccac ttcattctcc tttttctaac cagttttcca agacttgata aactcctcct
     6961 tggctgttgg ctggtgctag agcagtcgcc acagctttgg ctttctcatc agcattttcc
     7021 atcgcatagg caattccagc catttcaagc atttcaacat cattttcact atcaccaaaa
     7081 gccatgattt cttgggattt caagtcccag cgcttgagta attcctccaa gccccatgct
     7141 ttatgaatcc cagcttggat gatatcaatg caaccatagc cactggatac agctcggaca
     7201 cggccatcaa agagagcatt gatttcttcc aaaaccgaac tcaaacgttc ctcaccaaca
     7261 accatgctca tcttgagcac accaccaaag aggtcagatg ttaattcatc cacaaattgc
     7321 atccgttggt agaatttttc aatcatttct ggagtcataa aactttcaag atctgtaaaa
     7381 atcgtacctt ccttgacaaa atcacccttc atccccgtta caacaaactg gtcctgacac
     7441 gctcgaacct tgaaatgagt caaagccttg ttgacaatgg cgtcatccca tgtctgagcc
     7501 tgaatcaatt cattgttttc aaaaatacga gcgccattag caacaaccag aaccactcga
     7561 tccaccaagg gactcagtag ttgtctcatg cggtgaattt cattgcccgt cgcaatgaca
     7621 aaacgaatgc ccctttgatc caactgatct aaaatctttt ccaatcgtgg gagatcaagc
     7681 tgacctctag catccagcaa ggtcccatcc atatctgtcg caattacctt aatcatcatt
     7741 tactttcctc tggattcaag ctaataccac ttcaatccca catgttcatt catttcttta
     7801 taggctgtat caattcgttc cttagtcgct tcatctaact ggtcacgatg actgccatcc
     7861 tctataaaat cttccaaaat ataagtttgg taatgataca agggatatcc ttctggtgaa
     7921 aaggttccct ttggtgttcg attgacccaa gtaggatgag ctttagctgt tccgatagtt
     7981 gtttttccat ccttcttctt gatggtgaca tccatgagaa caccacgttc agtccactta
     8041 gcattctctt catctcccat agattcaatt cgttgattgg aaatgaagtt ccccatttaa
     8101 tagacaatga gcttcttatc tccatctttt tcaatcgttt cagatggttc aacaacgtaa
     8161 agatgccctc caaagataat atccgctccc caatcgatca tcttgtgata aagagctttt
     8221 tgttcttcag ttggttccaa tcgatactca acacccatct gaggcatgat aatggtgata
     8281 tctgcttcct tctctgcccg ttcaatttca gccttcatct tatcttcgtt taaatctgaa
     8341 agataacgat tatagtcttc ctgagaaata tactgctcaa ttccattgaa accataggaa
     8401 taggctaaca atgcaacctt gataccattc acttccttaa tgaccagcgg agcctgatca
     8461 cgtggttcgt gcgtataaac tccgattgga gtgattccag ctttctcaat aatatcggcc
     8521 gttgaaataa ctccctcaat ttgcgaatcc aaaatatgat tatgagctaa atccagcaca
     8581 tgataacctg cctccttaat agcatccata acttcagcag gagcattaaa gagaggataa
     8641 cccgctaaat aatgatcctt attaatggtt tcttcaaaat caccaatagc taaatctgct
     8701 tgcttgaacc aaggagtcac atactcaaaa ttttcatgaa agtcataggt accgtcttct
     8761 tttttagctg aaaagaaaag tccatcgtgg tagagcaaat ccccgtgggc cataattctg
     8821 gcagtttttt cctcttccta ctcctgagac ttttgcttag ttccctcttg agaaatagta
     8881 tctcgttttt gactagtcaa gagattcctt ggaagctttc aaacaataag accaagccca
     8941 ttgataaacc aactgctagc aagagtatcg ccacaaatcc cttattgctc cacttgcgat
     9001 aactcctaaa aagtttacca agcccttcat aaaacgaaaa gctaaaccac cctgatttcg
     9061 attttgtctt ctttgcatct tcattctccc tactttctta tgcaagcctt ttctttttat
     9121 tatatcacag ataagtattt ctttcacaat tgaattgaac ttcccatcta ttttctataa
     9181 atcctaaatg ccataatgct ttcaattctt gtcattttgt gatatcatgt agaggtaatg
     9241 aaaggaaatg aactaatcca cagtggctta ttccaagtat accacttggg ctttggcagt
     9301 agttaactgc actaaacata atataaggag agaaaatgtc tgcaatagaa cgtattacaa
     9361 aagctgctca cttaattgat atgaacgata ttatccgtga agggaatcct actctacgcg
     9421 cgattgctga ggaagtcact ttccccctat ctgaccagga aatcatccta ggcgaaaaga
     9481 tgatgcaatt ccttaaacat tcccaagatc ctgtcatggc tgaaaaaatg ggactccgcg
     9541 gtggtgttgg actggctgct ccccagttag atatctcaaa acgcattatc gctgttttgg
     9601 tacctaatat tgttgaagaa ggcgaaactc cacaggaagc ctacgatttg gaaggcatta
     9661 tgtacaatcc aaaaatcgtc tctcactctg ttcaagatgc tgctcttggc gaaggagaag
     9721 gttgcctgtc tgttgaccgt aacgtgcctg gctatgttgt tcgccatgcc cgcgttactg
     9781 ttgactactt tgacaaagat ggagaaaaac accgtatcaa actcaaaggc tacaactcca
     9841 ttgttgttca gcatgaaatt gaccacatta acggtatcat gttttacgat cgcatcaatg
     9901 aaaaagaccc atttgcagtt aaagatggtt tactgattct tgaataaaga aaatcccgtt
     9961 gcaagacggg gttttgtgtt ataatagagg catgaaaaca aatgatattg tctatggtgt
    10021 ccacgccgtt accgaagccc tccttgcaaa tacaggaaac aaactctacc tccaagaaga
    10081 tctccgaggt aagaatgttg agaaagtcaa ggaactagct acagaaaaga aggtgtccat
    10141 ttcttggaca tcaaaaaaaa atctctctga gatgactgaa ggtgctgttc atcaaggttt
    10201 tgttctacga gtgtctgaat ttgcctatag cgagctagat tacatccttg caaaaacacg
    10261 ccaagaagaa aattcacttc tattgattct agatggtcta accgatcccc ataatctggg
    10321 ttctatcttg cgaacagccg atgcgaccaa tgtttcaggt gtcatcattc ccaagcaccg
    10381 tgctgtcgga gtaactcctg tcgttgccaa aacagccaca ggtgctattg aacacgttcc
    10441 aattgcccga gtaaccaacc tcagtcaaac cttagataaa ctcaaggatg aaggtttctg
    10501 gacctttgga acggatatga acggtactcc ttgccacaag tggaatacaa aagggaaaat
    10561 cgccctcatc attggaaatg aaggaaaagg tatctctagc aacataaaaa aacaggtcga
    10621 tgaaatgatt accattccga tgaatggaca tgttcaaagc cttaatgcca gtgttgctgc
    10681 ggccattctc atgtacgaag ttttccgaaa tagactataa aaaagtttcc agtcatctga
    10741 ttggaaactt ttttatgatt aactatgttc tgtaatgaat ttataggctt cttgaccagc
    10801 gatagctcca tctccaactg ctgttgttac ttggcgaagg tctttcaagc gaacatctcc
    10861 aactgcaaag ataccgtcga ctgcagtctt catgtggcta tctgtcacaa tccagcctgc
    10921 ctgatcttgg atattcaatt ctttaacaaa atcgctaaga gggtccaaac caacatagat
    10981 aaagacacca ccgaaggctt gctctgtcac ttgacctgtt ttcacatttt caaaaacgac
    11041 agattctact ctgttttcac ccttgatttt ccttactaca gaatcccata taaagctgat
    11101 tttctcattg gcaaaggcgc gatcttgtaa aaccttttgg gcacgaagtt ggtcacgacg
    11161 gtgaacaatg gtaacagtct tagcaaaacg agtcaagaag agggcttctt caacagctga
    11221 atctccacca ccaactacca ataaatcttg gtcacggaag aaagcaccat cacacacagc
    11281 acagtaagaa acaccacgac tgttcagttc ttcttctcca ggcactccca aaggacggtg
    11341 tttagaacca gttgctacga taactgtacg tgtttcatat gtttggtcat cagtcatcac
    11401 tttcttaaaa tcaccatggt cttcgacatt ttcaacataa ccataaatgt gctcaacacc
    11461 aagattttca agtggttcaa acatcttttc agccaattca ggtccactaa tattagcgta
    11521 tcctgggtaa ttttcgatat cagatgtatt attcatctga ccacctggca gaccaccttc
    11581 aatcaaagct acttttagat tgcttcgagc agcatacaag gccgcagtca tccctgcagg
    11641 tccagcaccg ataataatag tatcgtacat atagattcct tctttcttgt tgtaactatc
    11701 tttattctaa ctctttcttg tcaatcacgc aaggattatg ccttgaaaac aagaattaaa
    11761 ctcataaccg caaaggataa tactgggaca accaagactc caatcataat catatcatag
    11821 agatgggctt ggcgagcctt ggctgtctta tcaactcccg acatggctct cagaccaatc
    11881 caaatcccta aaaaaatcag aacaaggatg gtggtcaaga tcaaactctc gaaatataaa
    11941 gaaaatagtt gcagtagcat gatttctctc atttctatct tttttaaaga gtaaactcag
    12001 ctagtccaac taactgagtt ttcctttatc tattatatca aatataagtc cgtttgtaac
    12061 tagcgaagaa ttcttttgtc cgctcttctt taggggtgtg gataatctca tccggagttc
    12121 cagactcgat gattttcccc ttatctaaga agagaatctt atccgcaact tgggctacaa
    12181 aggacatgtc atgactgacc aaaatcatgg tctgacctga cttagcagca tctgcaatag
    12241 acttttctac ttcaccgacc aattctgggt caagggctga agttggttcg tctaagagca
    12301 aaacatctgg tttcatagca agcgcacgcg ctagggcaac ccgttgcttc tgtccacctg
    12361 ataaatggcg aggataatgg ttttcacggt ccgaaagccc aaccttagcc aactcttcct
    12421 tggcaatctt agtcgcttct tggtcagata atttcttgac aacaaccaag ccttctttca
    12481 cattatcaag tgctgttcgg cgttcaaaca aattaaactg ttggaaaacc atagacaact
    12541 tacggcgtag ggcaaggatt tcttcttgag tgattttaga aaaatcaact gaaaaaccat
    12601 caatctgaat agagccactg tcaggtgttt caagataatt gagactgcga agaaaggttg
    12661 attttccagc tcctgaagaa ccaatcaagg ctacaacttc ccctttttga atatccaagt
    12721 ccagatgatc caagacagtc tgtcctgaaa aggatttgct taaattcgaa atcttaatca
    12781 ttaacgaagg tctcctttca catctgtttg cactgtatca ggtgcagaaa tagccatttt
    12841 tctctcgatg aaacgaccga gactttcaat tccgatattg actacccaat aaacaagggc
    12901 aacggagatg aagcgttcaa aatagcgata atcagctcca cctagaatct gagcttgggc
    12961 aaagacttcc acaacacccg cactaaaagc tagagatgtt cccttggtca aaccgatgag
    13021 ggaattaatc aaggttggag tagctaccac cgctgcatta ggaataatca ctcgtcgata
    13081 aacttgcgct cgggtcatac ccagactgcg tgccgcctca atctcaccag gattaactga
    13141 gagaatggct gcacgaatgg tttcactagc ataagctgcc tcattaaagg caaaggcgac
    13201 aatcgcaaaa gctgcagctg gaatcgcatt gatattgaga ccagttcccc attgctgatt
    13261 gagggctttc aaagccaaag ggattccgta gtaggtcaac atgagttgca ccaaaatcgg
    13321 tgtccctttt aagaaactaa caaagaaggc ctgcaaggga tataaaatct tgacacgatt
    13381 gatcttcaca atggcaaaaa gaagcgccaa aaccaagcca aaaagggcac caccaattgt
    13441 caacataatt gttgttggaa gttgttggac aattctaggg attccatcaa agaccgaacg
    13501 taggctaaac agcttgccat tcggaatcaa ttgcatcaag ttttggtacc aatctgatgc
    13561 taaaatcgtt gtaacattca taaaaacatc ctcttctgat aatgattcat tctaccatgc
    13621 ttctgccgtc aatgaaatta tttgctacgg tcagatacag actttgtcta cctactagtt
    13681 tatcatactt tgaaacaggg aagttatata ttttatctat caaagagata gataaaaact
    13741 atttcagtcc cagttcttca taagattttc gataaccgat ttgcttaacg gttccatttt
    13801 ctactaaaat gggccgtttt aacaacatac cgtcacttgc tagcaactca gccgcttctt
    13861 ggtttgacaa acttcctacc ttatctttta gccctaattc acggtatttg ataccactgg
    13921 tgttgaaaaa ttgcttcaat tcaaatcctg aggtttctag ccaattcaaa atgacttctt
    13981 ggctaggtgt ttcttccacg atatggacgg ctttatagtc cacacctaat tgatttaatt
    14041 cttgttttgc ttttttacaa gttgaacatt tggggtattc gataaattct aacatgtgct
    14101 tctccttcta ttgcttctat ctttaaaatc tactccttca tgctccaaga gccaagcttt
    14161 cttttccact cctgcagcat aacctgtcag acgcttgcct gctcccaaca cacgatgaca
    14221 aggtactagg atagaccaag gattgcgtcc cactgctcca ccaattgctt gagcagaagc
    14281 cacttgcagg tcttgagcaa tttgtccata ggtcactgtc tgaccataag gaatgccctg
    14341 taaatagtcc caaactctct tttcaaaatt cgttccgatt ggcgccaaga gcaagttgga
    14401 taaatcctga ggcttgcctt taaagtaatc atctaagcaa gcaataactg ggtctaaaat
    14461 aggatgacta acaacttctt ctatcgtttc atctcctagt cccctctcaa aatgcttctg
    14521 ctcctgaacc caaattccat acaaataatg gtcatcagct acaagtgata gaataccaat
    14581 tggtgaggag tagagtattt ttacatactt cttttgcatt atttctttaa tttttgatag
    14641 gccaagcgtt cgccatctac tggcacctta ccaacttgtt taaaaccaag tttttcaaaa
    14701 atatgttgca taaccttgtt ttcagcatgc gtatctgagc gaaaatcaag ataatcaaaa
    14761 ccttcaatca agccctctaa gaaggtttga gcaactcctt ttccctgcac atctgctgcc
    14821 acagcaatac ggtgaaagac tagatactct gactctccag cttgccagtt tccctcataa
    14881 atagcttcat aggcctcctc tggactcttg gtcacagcag cataggctag cagttctccc
    14941 tcttccaagg ctacgtaggc ttgccctgag atgatatcat cgataataac atctgcattt
    15001 ggatagccat tttgccactg gtcactacca gcatctgcta aacatttttt ggcatcctcc
    15061 atcacctgca tgatggcatc tacttcattt ggaaaagcta aacgaatttc catctttttt
    15121 cctcatttct gactagtttc tcccatcata gcataattta agtatttcta caagcagtta
    15181 aaacttgact ttgtgttttt gttattttat aatctagtta tcaaaactat ataaaaagga
    15241 gttgaaaatg aaaactacct tttcctaccc aaaatgggca gaaattccaa acattgacct
    15301 ctatctggac caggttttgc tctatgtcaa tcaggtctgc gcccctatct ctcctaataa
    15361 agacaagggc ctaacagcat ctatggtcaa caattatgtg aaaaatggtt acctgacaaa
    15421 gcctgacaaa aaaaaatacc aacgccaaca gattgcccgt ttgattgcta tcacaaccct
    15481 caagtctgta ttttctattc aagaaatagc ccagacactt aatactctac aaactcaagc
    15541 aagttcagac caactctacg atgcttttgt ggactacatg aaccaaggaa ttgacccagc
    15601 taaccctatt atccaaacca gctgccaaac cgttaaactc tatcatcaaa ctctagactt
    15661 aatcgatcat actcaagagg aggtaatcca atgaacacta gtcttaaact cagcaaacaa
    15721 ctcagttttg gagaggagat tgctaatagc gtgacccatg ctgtgggtgc agtcatcatg
    15781 cttatcttgc tgcctatttc atccatctat agttatgaag cacacggatt tttatcctct
    15841 atcggcgttt ccattttcgt catcagtctc tttctcatgt tcctatcatc caccatttat
    15901 cactctatgg cctatggttc gacccacaaa tatgttttgc gaatcattga ccattctatg
    15961 atttacgttg ccattgccgg ctcatacacg cccgttgtct tgaccttgat gaataactgg
    16021 tttggctatc tgattattgt catccaatgg ggaacgacca tctttggtat tctctataaa
    16081 atctttgcta aaaaggtcaa tgagaaattt agccttgctc tttacctgat tatgggctgg
    16141 ttggttctgg ctatcattcc tgccattatc agtcaaacga cacccgtttt ctggagtctc
    16201 atggtaactg gcggactctg ttatacagtt ggagctggat tttatgccaa gaaaaaacct
    16261 tatttccaca tgatttggca tctctttatc ctaactgcgt ccgtacttca atacatcgct
    16321 attgtttatt acatgtaaaa ctctcagtag gcagagcctg cttagagttt tacaaacgaa
    16381 tttcccaaag aggaagcaat atgaaagcag tcattgcttg tgttcataaa attctcagaa
    16441 tcatttacaa actactctcc accaagtaga cttatcaaaa agaaaaggcg ctaggactga
    16501 agaaacagtt ctaacgccca aattaaaaaa atttcaacta cagtatagca caggaaatga
    16561 ttttttgcgc ttttttactg ttttttcaaa taaaaaaaga caaagtttta tccttgtctt
    16621 ttagttttag tattgctttg gtttcaatgt caataagtgt gttc
//
LOCUS       DAVKMU010000042        16656 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKMU010000042 DAVKMU010000000
VERSION     DAVKMU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.042"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..1109
                     /gene="lytB"
                     /locus_tag="VW343_001599"
     CDS             <1..1109
                     /gene="lytB"
                     /locus_tag="VW343_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU9756127.1"
                     /translation="KMAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDG
                     KMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVV
                     PVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDAS
                     KDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLF
                     KDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESN
                     WGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGN
                     KASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(1170..2825)
                     /gene="pavA"
                     /locus_tag="VW343_001600"
     CDS             complement(1170..2825)
                     /gene="pavA"
                     /locus_tag="VW343_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9756128.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2930..3427
                     /gene="ybeY"
                     /locus_tag="VW343_001601"
     CDS             2930..3427
                     /gene="ybeY"
                     /locus_tag="VW343_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9756129.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3408..3803
                     /locus_tag="VW343_001602"
     CDS             3408..3803
                     /locus_tag="VW343_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9756130.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3820..4719
                     /gene="era"
                     /locus_tag="VW343_001603"
     CDS             3820..4719
                     /gene="era"
                     /locus_tag="VW343_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9756131.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4768..5592
                     /gene="mutM"
                     /locus_tag="VW343_001604"
     CDS             4768..5592
                     /gene="mutM"
                     /locus_tag="VW343_001604"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9756132.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5592..6197
                     /locus_tag="VW343_001605"
     CDS             5592..6197
                     /locus_tag="VW343_001605"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9756133.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            6184..7383
                     /locus_tag="VW343_001606"
     CDS             6184..7383
                     /locus_tag="VW343_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9756134.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7383..7532
                     /gene="rpmG"
                     /locus_tag="VW343_001607"
     CDS             7383..7532
                     /gene="rpmG"
                     /locus_tag="VW343_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9756135.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7572..7805
                     /gene="secG"
                     /locus_tag="VW343_001608"
     CDS             7572..7805
                     /gene="secG"
                     /locus_tag="VW343_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9756136.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KVRGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7907..10261
                     /gene="rnr"
                     /locus_tag="VW343_001609"
     CDS             7907..10261
                     /gene="rnr"
                     /locus_tag="VW343_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9756137.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAANYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10224..10691
                     /gene="smpB"
                     /locus_tag="VW343_001610"
     CDS             10224..10691
                     /gene="smpB"
                     /locus_tag="VW343_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9756138.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10707..11567
                     /gene="tehB"
                     /locus_tag="VW343_001611"
     CDS             10707..11567
                     /gene="tehB"
                     /locus_tag="VW343_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9756139.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            11640..12593
                     /gene="coiA"
                     /locus_tag="VW343_001612"
     CDS             11640..12593
                     /gene="coiA"
                     /locus_tag="VW343_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9756140.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            12612..14414
                     /gene="pepF"
                     /locus_tag="VW343_001613"
     CDS             12612..14414
                     /gene="pepF"
                     /locus_tag="VW343_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9756141.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            14416..15129
                     /locus_tag="VW343_001614"
     CDS             14416..15129
                     /locus_tag="VW343_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9756142.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            15196..16137
                     /gene="prsA"
                     /locus_tag="VW343_001615"
     CDS             15196..16137
                     /gene="prsA"
                     /locus_tag="VW343_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9756143.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFKKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(16254..16595)
                     /locus_tag="VW343_001616"
                     /pseudo
     CDS             complement(16254..16595)
                     /locus_tag="VW343_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5012 a         3099 c         3729 g         4816 t
ORIGIN      
        1 gcaaaatggc tgaaaaagaa tgggtctacg attctcatag tcaagcttgg tactacttca
       61 aatccggtgg ttacatgaca gccaatgaat ggatttggga taaggaatct tggttttacc
      121 tcaagtctga tggcaaaatg gctgaaaaag aatgggtcta cgattctcat agtcaagcct
      181 ggtactactt caaatctggt ggttacatgg cgaaaaatga gacagtagat ggttatcagc
      241 ttggaagcga tggtaaatgg cttggaggaa aagctacaaa tgaaaatgct gcttactatc
      301 aagtagtgcc tgttacagcc aatgtttatg attcagatgg tgaaaagctt tcctatatat
      361 cgcaaggtag tgtcgtatgg ctagataagg atagaaaaag tgatgacaag cgcttggcta
      421 ttactatttc tggtttgtca ggctatatga aaacagaaga tttacaagcg ctagatgcta
      481 gtaaggactt tatcccttat tatgagagtg atggccaccg tttttatcac tatgtggctc
      541 agaatgctag tatcccagta gcttctcatc tttctgatat ggaagtaggc aagaaatatt
      601 attcggcaga tggcctgcat tttgatggtt ttaagcttga gaatcccttc cttttcaaag
      661 atttaacaga ggctacaaac tacagtgctg aagaattgga taaggtattt agtttgctaa
      721 acattaacaa tagccttttg gagaacaagg gcgctacttt taaggaagcc gaagaacatt
      781 accatatcaa tgctctttat ctccttgccc atagtgccct agaaagtaac tggggaagaa
      841 gtaaaattgc caaagataag aataatttct ttggcattac agcctatgat acgacccctt
      901 acctttctgc taagacattt gatgatgtgg ataagggaat tttaggtgca accaagtgga
      961 ttaaggaaaa ttatatcgat aggggaagaa ctttccttgg aaacaaggct tctggtatga
     1021 atgtggaata tgcttcagac ccttattggg gcgaaaaaat tgctagtgtg atgatgaaaa
     1081 tcaatgagaa gctaggtggc aaagattagt actataagtg aatatgattt gagtgaatag
     1141 taagttaaaa atcctgattt caagtaaaat caggattttt tcatggatgc aatttttttg
     1201 gagtctggtg tgacgcggag ggtcttttgt cctgtgtaag tgacaaagcc gggttttcca
     1261 ccagttggtt tattgagttt tttgacttca atcatatcta cctgcaccag attcgacagg
     1321 cgcccttgag agaagtaggc agctaactct gctgcgtctg tcttgactgc atcagatggg
     1381 tcaagatttc ctgagatgac aacatggctt ccaggaatgt ccttagcatg gaaccaaagt
     1441 tcctccttgc gggccatttt aaaggtcaat tcctcatttt gaagattgtt tcgtccgaca
     1501 tagatgatgg ttttgccatc gcttgctaga tattgttcta gttttttgcg tttctggatt
     1561 ttctcccgtt gtcttctgcg gataaaacct gtttgaatca attcttcacg gatttcagcg
     1621 atttcttcca gtccagcttg gttgaggacg gtttctacac tttccagata gagaatagtg
     1681 gctttggttt cttcaatcaa atcagtcaag tatttgacag cttctttgag tttctgatac
     1741 cgtttaaaat agcgttgggc attctggttg ggagtcagag ccttatcaag cgcaatcatg
     1801 ataggttggt tggtatagta gttgtctagg ataacctggt cttggtcgtt aggcacttgg
     1861 tggaggaagg ttgtcagcaa ttctcctttt tgacgaaatt cttcagcgtt gtctgtcgcc
     1921 agtaactctt tttcctgttt tttgagtttg tgtcggtttt tctgaagttc attttcaaca
     1981 cgacgaatca gttcactggc ctgctgtttg acgcggtcgc gctcagcctt atccttatag
     2041 taggtgtcca acaaatcaga aagatttgca agaggctctc ccacctgatt tgcaaaagga
     2101 actggactga aggaagtctc agtcaagcat ggcttggttt cttgattgaa aaaatttcgg
     2161 aaagcggaaa gtttttcact aaccagtatc ctttccaatt catttgccgt atcgcgtccc
     2221 agaccttgaa agaggctttg aagatttttt gctgttagtt cttgggtttg caggatttca
     2281 aagagctttt catccttgat agtaaaagga ttgagagatt ttgtacttgg cggagcgata
     2341 taggtcgatc ctggaagtaa ggtgcggtag ctattttgtg aaaagccgac gtgtttgata
     2401 acttcgagga ttttatgact gcttttatcg accagtagaa tattactgtg tttccccata
     2461 atttcgataa tcaaggtagc ctggatatgg tctccaatct cgtttttatt ggaaactgta
     2521 atttccacaa tacggtcatt ttccacttgc tcaatcgact caatcagggc cccctgcaaa
     2581 tactttctca aaaccatgat aaaggtagaa ggttgggctg gattttcaaa agtcgtttgg
     2641 gtcagctgaa tgcgtccaaa aactggatgg gcagaaagga gcaggcgatg gctttggcga
     2701 ttgctgcgga tttgcaagac caactcttgt tcaaaaggct gattgatttt ctggatgcga
     2761 ccattcacta attcgcttcg caattcctca actatgtggt gtaaaaaaaa tccgtcaaat
     2821 gacatcgttc tctccttgtg attgtattcc atagtattat atcaaaaagg tagaataaaa
     2881 tcatggaaat gtggtataat aaagccaagt aaagagaaac gagaagcaca tgtatattga
     2941 aatggtagat gaaactggtc aagtttcaaa agaaatgttg caacaaaccc aagaaatttt
     3001 ggaatttgca gcccaaaaat taggaaaaga agacaaggag atggcagtca cttttgtgac
     3061 caatgagcgt agtcatgaac ttaatctggg gtaccgtgac accgaccgtc cgacagatgt
     3121 catcagcctt gagtataaac cagaattgga aattgccttt gacgaagagg atttgcttga
     3181 aaatccagaa ttggcagaga tgatgtctga gtttgatgcc tatattgggg aattgttcat
     3241 ctctatcgat aaggctcatg agcaggccga agaatatggt cacagctttg agcgtgagat
     3301 gggcttcttg gcagtacacg gctttttaca tattaacggc tatgatcact atactccgga
     3361 agaagaagcg gagatgttcg gtttacaaga agaaattttg acagcctatg gactcacaag
     3421 acaataaacg aaaatggaaa aatcgtgacc tgatatccag tttagaattt gccatcacag
     3481 ggatttttac tgctatcaag gaagaacgca atatgcgaaa acatgcagtg acggctctag
     3541 tggtcatcct tgcaggtttt ggttttcagg tgtcacgaat cgaatggctc tttctcctat
     3601 tgagcatttt cttggtggta gcctttgaaa ttatcaattc tgctatcgaa aatgtggtgg
     3661 atttggccag tcactatcac ttttccatgc tggctaaaaa tgccaaggat atggcggctg
     3721 gcgcggtatt agtggtttct ctttttgcag ccttaacagg cgcattgatt tttctcccac
     3781 gaatctggga tttattattt taaacagtaa gaggaaatta tgacttttaa atcaggcttt
     3841 gtagccattt taggacgtcc caatgttggg aagtcaacct ttttaaatca cgttatgggg
     3901 caaaagattg ccatcatgag tgacaaggcg cagacaacgc gcaataaaat catgggaatt
     3961 tacacgactg ataaggagca aattgtcttt atcgacacac cagggattca caaacctaaa
     4021 acagctctcg gagatttcat ggttgagtct gcctacagta cccttcgcga agtggacact
     4081 gttcttttca tggtgcctgc tgatgaagcg cgtggtaagg gggacgatat gattatcgag
     4141 cgtctcaagg ctgccaaggt tcctgtgatt ttggtggtga ataaaatcga taaggtccat
     4201 ccagaccagc tcttgtctca gattgatgac ttccgtaatc aaatggactt taaggaaatt
     4261 gttccaatct cagcccttca gggaaataac gtgtctcgtc tagtggatat tttgagtgaa
     4321 aatctggatg aaggttttca atatttcccg tctgatcaaa tcacagacca tccagaacgt
     4381 ttcttagttt cagaaatggt tcgcgagaaa gtcttgcacc taactcgtga agagattccg
     4441 cattctgtag cagtagttgt tgactctatg aaacgagacg aagagacaga caaggttcac
     4501 atccgtgcaa ccatcatggt cgagcgcgat agccaaaaag ggattatcat cggtaaaggt
     4561 ggcgctatgc ttaagaaaat cggtagcatg gcccgtcgtg atatcgaact catgctagga
     4621 gacaaggtct tcctagaaac ctgggtcaag gtcaagaaaa actggcgcga taaaaagcta
     4681 gatttggctg actttggcta taatgaaaga gaatactaag tagaggtggg ctcatgcctg
     4741 cttcttgttt ttacagaagg aggacttatg cctgaattac ctgaggttga aaccgtttgt
     4801 cgttgcttag aaaaattgat tataggaaag aagatttcga gtatagaaat tcgctacccc
     4861 aagatgatta agacggattt ggaagagttt caaagggaat tgcctagtca gattatcgag
     4921 tcaatgggac gtcgtggaaa atatttgctt ttttatctga cagacaaggt tttgatttcc
     4981 catttgcgga tggagggcaa gtatttttac tatccagacc aaggacctga acgcaagcat
     5041 gcccatgttt tctttcattt tgaagatggt ggcacgcttg tttatgagga tgttcgcaag
     5101 tttggaacca tggaactctt ggtgcctgac cttttagacg cctactttat ttctaaaaaa
     5161 ttaggtcctg aaccaagcga acaagacttt gatttacagg tctttcaatc tgcccttgcc
     5221 aagtccaaaa agcctatcaa atcccatctc ctagaccaga ccttggtagc tggacttggc
     5281 aatatctatg tggatgaggt tctctggcga gctcaggttc atccagctag atcttcccag
     5341 actttgacag cagaagaagc gacagctatt catgaccaaa ccattgctgt tttgagccag
     5401 gctgttgaaa aaggcggttc caccattcgg acctatacca atgcctttgg ggaagatgga
     5461 agcatgcagg attttcatca ggtctatgat aagactggtc aaggatgtgt acgctgtggt
     5521 accatcattg agaaaattca actaggtgga cgtggaaccc acttttgtcc aaactgtcaa
     5581 aggagggact gatgggaaaa atcatcggaa tcactggggg aattgcctct ggtaagtcaa
     5641 ctgtgacaaa ttttctaaga cagcaaggct ttcaagtagt ggatgccgac gcagtcgtcc
     5701 accaactaca gaaacctggt ggtcgtctgt ttgaggctct agtacagcac ttttggcaag
     5761 aaatcattct tgaaaacgga gaactcaatc gccctctcct agctagtctc atcttttcaa
     5821 atcctgatga acgagaatgg tctaagcaaa ttcaagggga gattatccgt gaggaactgg
     5881 ctactttgag agaacagttg gctcagacag aagagatttt cttcatggat attcccctac
     5941 tttttgagca ggactacagc gattggtttg ctgagacttg gttggtctat gtggaccgag
     6001 atgcccaagt agaacgctta atgaaaaggg accagttgtc caaagatgaa gctgagtctc
     6061 gtctggcagc ccagtggcct ttagaaaaaa agaaagattt ggccagccag gttcttgata
     6121 ataatggcaa tcagaaccag cttcttaatc aagtgcatat ccttcttgag ggaggtaggc
     6181 aagatgacag agattaactg gaaggataat ctgcgcattg cctggtttgg taattttctg
     6241 acaggagcca gtatttcttt ggttgtacct tttatgccca tcttcgtgga aaatctaggt
     6301 gtagggagtg agcaagtcgc tttttatgca ggcttagcaa tttctgtctc tgctatttcc
     6361 gcggcgctct tttctcctat ttggggtatt cttgctgaca aatacggccg aaaacccatg
     6421 atgattcggg caggtcttgc tatgactatc actatgggag gcttggcctt tgtcccaaat
     6481 atctattggt taatctttct tcgtttacta aacggtgtat ttgcaggttt tgttcctaat
     6541 gcaacggcac tgatagccag tcaggttcca aaggagaaat caggctctgc cttaggtact
     6601 ttgtctacag gcgtagttgc aggtactcta actggtccct ttattggtgg ctttatcgca
     6661 gaattatttg gcattcgtac agttttctta ctggttggta gttttctatt tttagctgct
     6721 attttgacta tttgctttat caaggaagat tttcaaccag tagccaagga aaaggctatt
     6781 ccaacaaagg aattatttac ctcggttaaa tatccctatc ttttgctcaa tctcttttta
     6841 accagttttg tcatccaatt ttcagctcaa tcgattggcc ctattttggc tctttatgta
     6901 cgcgatttag ggcagacaga gaatcttctt tttgtctctg gtttgattgt gtccagtatg
     6961 ggcttttcca gcatgatgag tgcaggagtc atgggcaagc taggtgacaa ggtgggcaat
     7021 catcgtctct tggttgtcgc ccagttttat tcagtcatca tctatctcct ctgtgccaat
     7081 gcctctagcc cccttcaact aggactctat cgtttcctct ttggattggg aaccggtgcc
     7141 ttgattcccg gggttaatgc cctactcagc aaaatgactc ccaaagccgg catttcgagg
     7201 gtctttgcct tcaatcaggt attcttttat ctgggaggtg ttgttggtcc catggcaggt
     7261 tctgcagtag caggtcaatt tggctaccat gctgtctttt atgcgacgag cctttgtgtt
     7321 gcctttagtt gtctctttaa cctgattcaa tttcgaacat tattaaaagt aaaggaaatc
     7381 tagtgcgagt aaaaattaat ctcaaatgct cctcttgtgg cagtatcaat tacctaacca
     7441 gtaaaaattc aaaaacccat ccagacaaga ttgaggtttt aaagtattgt cccaaggaaa
     7501 gaaaagtaac cctacatctt gaatctaagt agattttatg gtaaaataat agggatttta
     7561 aggagtttga tatgtataac ctattattaa ccattttatt agtattatct gttgtgattg
     7621 tgattgcaat tttcatgcaa ccaaccaaaa accaatccag caatgtattt gatgccagtt
     7681 caggtgattt gtttgaacgt agtaaagttc gcggttttga agctgtaatg cagcgtttga
     7741 cagggatttt agtctttttc tggctagcca ttgccttagc attgacggta ttatcaagta
     7801 gataagaaaa taatgggcag gactaggtct ttgcctcttt ttatttttaa aggatgtttg
     7861 agaaggtttt acagtaaaag aaaattaaaa aatctagaaa gaaaatatga aagatagaat
     7921 aaaagaatat ttacaagaca agggaaaggt gactgttaat gatttggctc aggctttggg
     7981 aaaagacagt tccaaggatt ttcgtgagtt gattaaaacc ttgtccttaa tggaaagaaa
     8041 gcaccaaatt cgttttgaag aagatggtag tctgacatta gaaattaaga aaaaacatga
     8101 gattaccctc aaggggattt ttcatgccca taaaaatggc tttggctttg ttagtctgga
     8161 aggcgaggag gacgaccttt ttgtagggaa aaatgatgtc aactatgcta ttgatggtga
     8221 taccgtcgag gtagtgatta agaaagtcgc tgaccgcaat aagggaacag cagcagaagc
     8281 caaaattatt gatatcctag aacacagttt gacaacagtt gtcgggcaaa tcgttctgga
     8341 tcaggaaaaa cctaagtatg ctggctatat tcgttcaaaa aatcagaaaa tcagtcaacc
     8401 gatttatgtt aagaaaccag ccctaaaatt agaaggaaca gaagttctca aagtctttat
     8461 cgataaatac ccaagcaaga aacatgattt ctttgtcgcg agtgttctcg atgtagtggg
     8521 acactcaacg gatgtcggaa ttgatgttct tgaggtcttg gaatcaatgg acattgtatc
     8581 cgagtttcca gaagctgttg ttaaggaagc agaaagtgtg cctgatgctc cgtctcaaaa
     8641 ggatatggaa ggtcgtctgg atctaagaga tgaaattacc tttaccattg acggtgcgga
     8701 tgccaaggac ttggacgatg cagtgcatat caaggctctg aaaaatggca atctggagct
     8761 tggggttcac atcgcagatg tttcttatta tgtgaccgag gggtctgccc ttgacaagga
     8821 agcccttaac cgtgcgactt ctgtttacgt gacagaccga gtggtgccaa tgcttccaga
     8881 acgactatca aatggcatct gctctctcaa tccccaagtt gaccgcctga cccagtctgc
     8941 tattatggag attgataaac atggtcgtgt ggtcaactat accattacac aaacagttat
     9001 caagaccagt tttcgtatga cctatagcga tgtcaatgat atcctagctg gcgatgaaga
     9061 aaagagaaaa gaatatcata aaattgtatc aagtatcgaa ctcatggcca agcttcatga
     9121 aactttagaa aacatgcgtg tgaaacgtgg agctctcaat tttgatacca atgaagcgaa
     9181 gattttagtg gataaacaag gtaagcctgt tgatatcgtt cttcggcagc gtggtattgc
     9241 cgagcggatg attgagtctt ttatgttgat ggctaatgaa acagttgccg aacatttcag
     9301 caagttggat ttgcctttta tctatcgaat tcacgaggag cctaaggctg aaaaggttca
     9361 gaagtttatt gattatgctt cgagttttgg cttgcgcatt tatggaactg ccagtgagat
     9421 tagtcaggag gcacttcaag acatcatgcg tgctgttgag ggagaacctt atgcagatgt
     9481 attgtccatg atgcttcttc gctctatgca gcaggctcgt tattcggagc acaatcacgg
     9541 ccactatgga ctagctgcta actattatac tcactttacc agtccaattc gtcgttatcc
     9601 agaccttctt gttcaccgta tgattcggga ttacggccgt tctaaggaaa tagcagagca
     9661 ttttgaacaa gtgattccag agattgcgac ccagtcttcc aaccgtgaac gtcgtgccat
     9721 agaagctgag cgtgaagtcg aagccatgaa aaaggctgag tatatggaag aatacgtggg
     9781 tgaagagtat gatgcagttg tatcaagtat tgtcaaattc ggtctctttg tcgaattgcc
     9841 aaacacagtt gaaggcttga ttcacatcac taatctacct gaattttatc atttcaatga
     9901 gcgtgatttg actcttcgtg gagaaaaatc aggtatcact ttccgagtgg gtcagcagat
     9961 ccgtatccgt gttgaaagag cggataaaat gactggagag attgattttt cattcgtacc
    10021 tagtgagttt gatgtgattg aaaaaggctt gaaacagtct agtcatagtg gcagagggcg
    10081 tggttcaaat cgtcgttcgg ataagaagga agacaagaga aaatcaggac gctcaaatga
    10141 taagcgtaag cattcacaaa aagacaagaa gaaaaaagga aagaaacctt tttacaagga
    10201 agtagctaag aaaggagcca agcatggcaa agggcgaggg aaaggtcgtc gcacaaaata
    10261 aaaaggcacg ccacgactat acaatcgtag atacgctaga ggcagggatg gtcctgactg
    10321 gaactgaaat caagagtgta cgagctgctc gaattaatct caaggatggc tttgctcaag
    10381 tgaaaaatgg agaagtttgg ctgagcaatg ttcatatcgc gccttacgaa gagggcaata
    10441 tctggaacca ggaaccagaa cgtcgtcgta aactcctgct ccataaaaag caaattcaaa
    10501 aattggaaca agagaccaaa gggacaggaa tgaccttagt tccccttaag gtctatataa
    10561 aagatggcta cgctaagctt cttttaggac ttgccaaagg gaagcatgac tatgacaaac
    10621 gggagtctat caaacgtcgt gagcaaaatc gagatatcgc gcgtgtgatg aaagctgtta
    10681 atcagcgata aaaagaggaa ttgaaaatgg aaaaattagt tgcctataaa cgcatgcctt
    10741 tgtggaataa acaaacaatg cctgaagctg ttcagcaaaa gcacaataca aaagttggga
    10801 cttgggggaa aattactgtc ttgaagggag ctctcaagtt tattgaattg acagaagaag
    10861 gggaagttct agctgaacac ctctttgaag caggggcaga caatccaatg gcccaacctc
    10921 aagcctggca ccgagtggaa gctgccacag atgatgtgga atggtacttg gaattttatt
    10981 gtaaacctga ggattatttt gctaaaaaat acaataccaa tcctgttcat tcagaggtcc
    11041 tagaggccat gcagacagtg aaacaaggga aagctttgga tttgggttgt ggtcaggggc
    11101 gtaattctct ttttctagcc cagcaagatt ttgatgtgac ggctgtagat caaaatggac
    11161 tagctcttga aatcttgcaa agcattgtgg agcaggaaga tttggacatg cctgttggcc
    11221 tttacgatat caattcagct agcattgaac aagaatatga ttttatcgtt tcaacagttg
    11281 ttctcatgtt tctacaagcg gaccgcattc cagctattat tcaaaatatg caggagaaaa
    11341 ccagtgttgg tggttacaac cttatcgttt gtgccatgga cacggaggat tatccttgct
    11401 cggttaattt cccattcacc tttaaagaag gagaactggc agactattac aaggattggg
    11461 aattggttaa gtacaatgaa aatccaggcc atttgcaccg tcgcgatgag aatggcaatc
    11521 gtattcaact acgctttgcg accttactag ctaagaaaat caagtaaaca cacatgaaga
    11581 ttaggaattt tcctgatctt ttttcttttt tacgaatgat atagaaaagg agggaattca
    11641 tgtttgttgc gagagatgct aggggagaat tggtaaatgt gttagaggat aaacttgaga
    11701 agcaagcata cacctgccca gcttgtggag gccagctcca tttgcgtcaa ggaccaagtg
    11761 tacggacgca ttttgcccat aaatccttaa aagactgtga ttttttcttt gaaaatgaaa
    11821 gtccagaaca cctggccaat aaggaatccc tctatcactg gttgaaaaaa gagacaaagg
    11881 ttcaattaga gtacccgctt tcagaactta aacagattgc ggatgtattt gtaaatggca
    11941 atctagctct agaagttcag tgtagtccct tgcctcagaa agtccttaaa gagcgaagtg
    12001 agggatatcg tagtcagggt taccaagtac tgtggttgct gggtcaaaaa ctgtggctca
    12061 aggagcgttt gactcgtcta cagcaaggtt ttctttattt cagtcaaaac atgggctttt
    12121 atgtttggga attagacaag gaaaaacaag ttttaagact caaatacctg atttaccagg
    12181 atctccgcgg taaactccat tatcaaatca aggaattttc ctatggtcaa ggtagtttat
    12241 tggaaatatt gcgtcttccc tataagaaac aaaaaatatc tcattttaca gtttctgagg
    12301 acaaggacat ctgtcgctat atccggcaac aactttatta tcaaaatctc ttttggatga
    12361 aagaacaagc agaagcctat caaaagggag aaaatatcct gacttatgga ctgaaagaat
    12421 ggtatccaca aattcgacca atagtgggca aatttttcca gattgaacaa gacttgacta
    12481 gctattatca gcacttttat acctattacc aaaaaaatcc tcaaaatgat tggcaaaagc
    12541 tttatccacc agccttttat cagcaatatt tcttgaaaaa tatggtagaa tagaaaggat
    12601 ggaggaatct aatggtatta caaagaaatg aaataaatga aaaagataca tgggatctat
    12661 caacgatcta cccaactgac caggcttggg aagaagtctt aaaagattta acagaacaat
    12721 tggagacagt agcccagtat gaaggccatc tcttggatag tgcggataac ctactagaaa
    12781 tcactgaatt ttctcttgaa atggaacgcc agatggagaa gctttacgtt tatgctcata
    12841 tgaagaatga ccaggataca cgtgaagcta agtatcaaga gtactatgcc aaggccatga
    12901 cattctacag ccagttagac caagcctttt cattctatga gcctgaattt atggagatta
    12961 gcgaaaagca gtatgctgac tttttagaag ctcaaccaaa gctgcaggtt tatcaacact
    13021 attttgacaa gctcttgcaa ggcaaggatc acgttctctc acaacgtgaa gaagaattat
    13081 tggctggagc tggagaaatc tttggttcag caagtgaaac cttcgctatc ttggacaatg
    13141 cggatattgt gttcccttat gtcctagacg atgatggtaa agaagttcag ctatctcatg
    13201 ggatttacac acgtttgatg gagtctaaaa aacgtgaggt tcgccgtggt gcctatcaag
    13261 ctctttatgc gacttacgaa caattccaac acacctatgc caaaaccttg caaaccaatg
    13321 ttaaggtgca aaattaccgt gctaaagttc gtaactacaa gagtgctcgt catgcagccc
    13381 tcgcagcgaa ttttgttcca gaaagtgttt atgacaattt ggtagcagca gttcgcaagc
    13441 atttgccact cttacatcgc tatcttgagc ttcgttcaaa aatcttgggg atttcagatc
    13501 tcaagatgta cgatgtctac acaccgcttt catctgttga atacagtttt acctaccaag
    13561 aagccttgaa aaaagcagaa gatgctttgg cagtcttggg tgaggattac ttgagccgtg
    13621 ttaaacgtgc cttcagcgag cgttggattg atgtttacga aaatcaaggc aagcgttcag
    13681 gtgcctactc tggtggttct tacgatacca atgcctttat gcttctcaac tggcaggaca
    13741 atctggacaa tctctttact cttgttcatg aaacaggtca tagtatgcat tcaagctata
    13801 ctcgtgaaac tcagccttat gtttacggag attactctat ctttttggct gagattgcct
    13861 caactaccaa tgaaaatatc ttgacggaga aattattgga agaagtggaa gacgacgcaa
    13921 cacgctttgc tattctcaat aacttcctag atggtttccg tggaacagtt ttccgccaaa
    13981 ctcaatttgc tgagtttgaa cacgccattc accaagcaga tcaaaatggg gaggtcttga
    14041 caagcgattt cctaaataaa ctctacgcag acttgaacca agagtattat ggtttgagta
    14101 aggaagacaa tcctgaaatc caatacgagt gggctcgcat tccacacttc tactataact
    14161 actatgtata tcaatattca actggctttg cggccgcctc agccttggct gaaaaaattg
    14221 tccatggtag tcaagaagac cgtgaccgct atatcgacta cctcaaggca ggtaagtcgg
    14281 actatccact taatgtcatg agaaaagctg gtgttgatat ggagaaggaa gactacctca
    14341 acgatgcctt tgcagtcttt gaacgccgtt taaatgagtt tgaagccctt gttgaaaaat
    14401 taggattggc ataaaatggt tgaatcgtat agtaagaatg ctaaccataa catgcgtcgt
    14461 cctgtcgtca aagaagaaat tgtagacttg atgcgtcagc gtcaaaagca ggtcacaggt
    14521 ttcttgaaag aattggaaaa ctttgcccgc aaggaaaata ttcctattat tccccatgaa
    14581 acggttgctt atttccgttt tcttatggaa accatgcagc ctaaaaatat tctggaaatt
    14641 gggacggcta tcggtttttc agctctcttg atggctgaac atgcgccaaa tgctaagatt
    14701 acaactattg atcgtaatcc agaaatgatt ggttttgcca aggaaaattt tgcccagttt
    14761 gacagtcgca agcaaatcac tctcctagag ggagatgcgg tggatgtctt atctacactg
    14821 acagagtctt atgatttcgt ctttatggat tctgccaagt ctaaatacat cgtctttctg
    14881 ccagaaatcc tcaaacattt ggaagttggt ggtgtggttg tcttggatga tatttttcaa
    14941 ggtggtgatg ttgccaagga tattatggaa gtccgtcgtg gtcagcgaac catttatcga
    15001 ggccttcaaa aattatttga tgcaacctta gacaatccag aactcaccgc aacattagtg
    15061 cctttaggag atggtattct catgcttcgt aaaaatgtag cagatgttca actgtctgaa
    15121 agcgaatgat tttcagaaaa atttaagaaa aaatagtaaa atagatagag taacacttat
    15181 ctcaaaggag tagacatgaa gaaaaaatta ttggcaggtg ccatcacact attatcagta
    15241 gcaactttag cagcttgttc gaaagggtca gaaggggcag accttatcag catgaaaggg
    15301 gatgtcatca cagaacatca attttatgag caagtgaaaa acaacccttc agcccaacaa
    15361 gtcttgttaa atatgaccat ccaaaaagtt tttaaaaaac aatatggctc agagcttgat
    15421 gataaagagg ttgatgatac tattgccgaa gaaaaaaaac aatatggcga aaactaccaa
    15481 cgtgtcttgt cacaagcagg tatgactctt gaaacacgta aagctcaaat tcgtacaagt
    15541 aaattagttg agttggcagt taagaaggta gcagaagctg aattgacaga tgaagcctat
    15601 aagaaagcct ttgatgagta cactccagat gtaacggctc aaatcatccg tcttaataat
    15661 gaagataagg ccaaagaagt tctcgaaaaa gccaaggcag aaggtgctga ttttgctcaa
    15721 ttagccaaag ataattcaac tgatgaaaaa acaaaagaaa atggtggaga aattaccttt
    15781 gattctgctt caacagaagt acctgagcaa gtcaaaaaag ccgctttcgc tttagatgtg
    15841 gatggtgttt ctgatgtgat tacagcaact ggcacacaag cctacagtag ccaatattac
    15901 attgtaaaac tcactaagaa aacagaaaaa tcatctaata ttgatgacta caaagaaaaa
    15961 ttaaaaactg ttatcttgac tcaaaaacaa aatgattcaa catttgttca aagcattatc
    16021 ggaaaagaat tgcaagcagc caatatcaag gttaaggacc aagccttcca aaatatcttt
    16081 acccaatata tcggtggtgg agattcaagc tcaagcagta gtacatcaaa cgaatagtcc
    16141 aaatcaatga gtcagggaaa aaaactcgac ttcaggaaaa aatgaagcaa acattcccac
    16201 aataaaacgc atagtacaag gtttgtactg accccaaaag ttagacaatt aatttatctg
    16261 aaggatttag ttctgtattg cacagggcta agtcctttta gttttacctt aattctctta
    16321 ttgttgtaat aatcaatata gtctataatg gcttgttcca attgattaag tgatttaaat
    16381 gttttctcat agccataaaa catttcggat tttaaaatgc caaagaaaga ttccatccta
    16441 ccgttgtctt ggctgtttcc tttgcgtgac atagatgctt gaattccctt actctctagg
    16501 aagcgatgat aagaatcgtg ttggtattgc cagccttggt cactatggag aatcgtattc
    16561 tcgtagtgct tctctttgaa tgcctgttcc aacattaacg atcaatcaat ttaatcatgt
    16621 acctaatatt agaattgttt atcccaaatt tatttg
//
LOCUS       DAVKMU010000043        16200 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKMU010000043 DAVKMU010000000
VERSION     DAVKMU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.043"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(150..2255)
                     /locus_tag="VW343_001617"
     CDS             complement(150..2255)
                     /locus_tag="VW343_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9756144.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     TKHLEADMEDGVLVIREKA"
     gene            complement(2536..2724)
                     /locus_tag="VW343_001618"
     CDS             complement(2536..2724)
                     /locus_tag="VW343_001618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756145.1"
                     /translation="MLEIDLTVLIDLSCSYFVLIYSFEMVDRTDEVSSKHGFEVVDKE
                     KLMWFEEVFEECKKILVS"
     gene            complement(2864..3844)
                     /locus_tag="VW343_001619"
     CDS             complement(2864..3844)
                     /locus_tag="VW343_001619"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9756146.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(3846..6098)
                     /gene="pbpX"
                     /locus_tag="VW343_001620"
     CDS             complement(3846..6098)
                     /gene="pbpX"
                     /locus_tag="VW343_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9756147.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(6102..6419)
                     /gene="ftsL"
                     /locus_tag="VW343_001621"
     CDS             complement(6102..6419)
                     /gene="ftsL"
                     /locus_tag="VW343_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9756148.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(6431..7381)
                     /gene="rsmH"
                     /locus_tag="VW343_001622"
     CDS             complement(6431..7381)
                     /gene="rsmH"
                     /locus_tag="VW343_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9756149.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            7547..7741
                     /locus_tag="VW343_001623"
     CDS             7547..7741
                     /locus_tag="VW343_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9756150.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            7757..8299
                     /locus_tag="VW343_001624"
     CDS             7757..8299
                     /locus_tag="VW343_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9756151.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNIIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            8310..8552
                     /locus_tag="VW343_001625"
     CDS             8310..8552
                     /locus_tag="VW343_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756152.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            8563..8898
                     /locus_tag="VW343_001626"
     CDS             8563..8898
                     /locus_tag="VW343_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756153.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(9008..10009)
                     /locus_tag="VW343_001627"
     CDS             complement(9008..10009)
                     /locus_tag="VW343_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9756154.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(10069..11970)
                     /locus_tag="VW343_001628"
     CDS             complement(10069..11970)
                     /locus_tag="VW343_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9756155.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHYSKDYEKVKRSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKSYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIRVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(11992..12285)
                     /locus_tag="VW343_001629"
     CDS             complement(11992..12285)
                     /locus_tag="VW343_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9756156.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(12285..13103)
                     /locus_tag="VW343_001630"
     CDS             complement(12285..13103)
                     /locus_tag="VW343_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9756157.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(13090..13869)
                     /locus_tag="VW343_001631"
     CDS             complement(13090..13869)
                     /locus_tag="VW343_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9756158.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(13884..14375)
                     /locus_tag="VW343_001632"
     CDS             complement(13884..14375)
                     /locus_tag="VW343_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9756159.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(14386..15576)
                     /locus_tag="VW343_001633"
     CDS             complement(14386..15576)
                     /locus_tag="VW343_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEU9756160.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(15588..16022)
                     /locus_tag="VW343_001634"
     CDS             complement(15588..16022)
                     /locus_tag="VW343_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9756161.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         4714 a         3659 c         2861 g         4966 t
ORIGIN      
        1 ttttttcagc tggctccttc ttttttatcc tcaaaattct atcttatact caatgaaaat
       61 caaagagcaa actaggaaac tagccgcagg ctgtacttga gtacggcaag gcgacgttga
      121 cgtggtttga atttgatttt cgaagagtct taggctttct cacgaataac caaaacgcca
      181 tcttccatat ctgcttccag atgtttagta tctaaatgat ccaagtggaa gtctgtcacc
      241 ttatcacgaa tttcttgttc aaccacgcga cggagaggac gaacccccat gacttcgtcg
      301 taaccttctt ctgtgatata atctttagcc gcttgactga ctaccaagtc aatgtctttc
      361 ttagccaagg tttggttaac ttcagccaac atcaaatcta caatcttaga aaggtcttcc
      421 ttagtcaatt gtgagaactc gatgactgca ttaaagcggt tgaggaattc tggacggaag
      481 aagggtttca aacggtccat caattctggt ttatccgcat cttctgtcaa gttggcttca
      541 tagccaaatc cagcatttga ggtcgcaata atgacagtgt tcttgaagtt tactgtattt
      601 ccttgaccat ctgtcaaacg accatcatct agaacttgga ggagaagggt aataacttga
      661 ggatcagcct tttcaatttc atccaagaga atgatagagt atggattgcg acgaacacgt
      721 tctgttaagg tattgctatt gtcatcataa cccacatagc ctgctgttgt accaattagc
      781 ttagaaacag ctgtgcggtc actgtattca gacatatcca aacggataat cgcatcctgg
      841 gttccaaaca tatcgagtgc caattgctta gcaagctccg tcttaccaac cccagtagac
      901 cctacaaaga ggaagctgcc gattgggcga tttccttcat caaaaccagc acggttacga
      961 cggatagcgc gagctacagc ttctacggcc ttatcttgac caatcacctt gtcttgcaag
     1021 cgatgagcca tatctttcaa acgttcgata tctgaagctc ccatttgcga tactgggata
     1081 cctgtcattc gttccacaga ttcagccaca tcgttgacac ttgcagtcac tttcatatct
     1141 tctgtgtggt tttcgatttt cttttccaat tctgcaatgc gtgttttata gtttagagct
     1201 gcttcaaaat cttctgcttc aactgctttt tcttgcttgt ctttttccgt ttcgatttct
     1261 cgttcaacag catgcacatc tgttactgga tgttgagccg ccaagtgagc agccgttaca
     1321 tcgacaaggt caatagcctt atctggcaag ctacgttgag gaatgtattg aacagaataa
     1381 tccaccgctg ctttcaagac ttcgtctggc aagatgacat tgtggtgttg ttgatagagg
     1441 tcacgaattc cttgaagaat tttaaaagta ttctctgccg aaggagcatt gaccttcact
     1501 tcgttgaaac gacgagcaag agcagcattc ttcaagatgg tgttacggta ttcgtcttga
     1561 gttgttgccc caatcactgt caattctcca cgagagagag ctggcttgag aatatccgca
     1621 agtcctttag aaccactgtc tccaccagtg ctaccagcac caagaatttg gtgaatttca
     1681 tcaaagaaga ggataatatt ccctgcttct ttcacttcat tgactaagtt ttggacattt
     1741 tcttcaaagc taccacggta ttgagtacca gcctcaagac ctgagatatc aatagaaata
     1801 atttccttgt tcttgatagc agcaggaaca tctccgttca caatggcttg cgctagacct
     1861 tcgacaactg ctgtcttacc aacacctgca tctccgacca aaacaggatt gttcttggtg
     1921 cggcgtgaga ggatttcaga tgtttcttga atttccttgt ttcgtccgat aacaggatcc
     1981 aacttgccct cacgcgcttc tgctgttaag tttcgaccta gttttgcaag gacaccgtct
     2041 tgtttcatac ctgatgcctg ttgtggcatt tgcacatcag tttctgcatt tcctggtaat
     2101 tgaccagtcg cacgatagtg agcaaattcc tcaggtgtga cttcgcgtcc attaattaag
     2161 taacggcgat tttcagaact gtatcctcgc ataccaccca tcaattggtt aaataaatca
     2221 tccatgttat taaaattatt aaagttgtta ttcatattct ttacctcttt ttgtttactt
     2281 agttatgatt actgatattg actatctttg accattgttt taaaaaattt agactagcta
     2341 gatcgctacc ctacgtacta tttctggttt ttctttttca agcaggagta gactatcttt
     2401 acttctgaag atttctagat gaaacataga cccacctctt tctattttac tttaaataag
     2461 tgttctagta aggctttcat ttaccttacg ttcataatat actacattgg tcagaaaagg
     2521 tcaagagttt tgactttaac tgaccaatat ttttttacac tcttcgaaaa cctcttcaaa
     2581 ccacatcagc ttctccttat ctacaacctc aaaaccgtgt tttgagctga cttcgtcagt
     2641 tctatcaacc atctcaaaac tgtatattaa aacgaaataa gaacaggaca aatcaatcag
     2701 gacagtcaaa tcgatttcta acaatatttt agaagtagat gtgtactatt ctagtttcaa
     2761 tctactatat tttgagcagc ctaaagttag ctttctagtt tgttctttga ttttcattgt
     2821 gtatttaaac acaaaaacac cctgaaacat cagggtgcca ttcttacatc aaatataaaa
     2881 ttgctagggt caggagactt gctagaagtc ccactcccca aaagaagaag tcaaccttcc
     2941 actcgctcca aggatttcct ttaccagaca atcccccaag ctcaaaatgg tgatgtacag
     3001 gcgtcatacg gaaaatacgt ttaccacctg tcagtttgaa ataactgact tgcatcataa
     3061 cagaagttgt ttcaaaaaca tacacaattc cgataatcaa gagagtccat tcttggtgga
     3121 gagccataga gatagctgcc agcatcccac ctagggccaa acttcccaca tcacccataa
     3181 agaccttggc aggcttatgg ttaaagatga agaaaccgag caaaccacca atcatggcaa
     3241 gaatcactag aagaatatcc atctgacctt gcacataggc aataactcca taggcagaca
     3301 aactaatcac aacggaaata ctagctaaac cgtcaacacc gtctgtcaag tttactgcgt
     3361 ttgaaaaacc gactagccag aaaagagcga agaaaatata gaaaaatccc aaatgaactg
     3421 gataaccaaa gacagacagg atatcgccac cgcgctcata gaaaagatag aagataactc
     3481 cacctagaag ctgaagagct aatttttgct taggattaag cccctcattg attttacgaa
     3541 agaccttgag aaagtcatct aaaaatccga ccaagccata caagaccaag atgaacaaaa
     3601 tcattcccac attattgctg aattggctac taaatagggc gaaaaagaaa gcaaccaaaa
     3661 cagaagtaat caagaaaacc aaacctccca ttgtaggagt cccagctttt gcctgatgct
     3721 gtttgacatc ttcatgcatc tgctgtcctg taatttgcgc ctttctataa aattggataa
     3781 aggccggaat tcctactaaa gttagtaaaa atgtcacaat tccagcactg atggaaataa
     3841 acatattagt ctcctaaagt taatgtaatt tttttaatgt ccttgatagc tgtgttagca
     3901 cgaacatctt gcttctgcac agtagagccc gaaccttgaa attcaagttc tatattgagc
     3961 cacttagcaa gggtctcagc agtctccttt gtccaaccat acatatctgg aacttcctct
     4021 gctttatcag ataagataag gacttgctga ttcggggcaa gattcttccc ttcttcagca
     4081 gaactgtttt taatcttcgt tcctgttccc acaacgatgg gttgtacaag attgcgacgc
     4141 aattcttctg ctaaatcacc aggtgaaata tccttgacac taggcatagg ataaggactt
     4201 tgttgactta cttgctccaa agctttagct gttgtttgaa gattgagaga gtctttcata
     4261 gctgaagccc gctccaagat aggattggca aattctccca actgaatacc tgaataatgt
     4321 tcaggttgtt ggaccgtcac atacaagata aaatcaggat tttcagccgg actcatcgat
     4381 acagccgaga aaatatagtc ggttaccccg actagataac caccattttt ctcgtcagca
     4441 atctgagccg taccagactt gagggctaca ttttgcccag gaacagttac agttggcttg
     4501 cctgtgctgt ggttatacat ggttccataa accggatccg tccctaccaa aaccatgtta
     4561 gtccgagtta gactagctgc atctttagaa acaggatttc ccacaatttc tttttgagat
     4621 ttccgagcag tttgatcatt tggatcataa atggcactaa taaatttagg ctccagcatg
     4681 acaccgtcat tagcaatagc tgtaaaggca cgaatcattt gcgtctgggt cactgaaatc
     4741 ccttgtccaa atgagctttg cgcaatgttg acaatattat ccgcaggaag ctgaccagca
     4801 tactcatccg tcaaaccgaa acgggtcgga actccaaatt taaaacgatt aagataatca
     4861 agccaggtag catctcccat cttttgctca aggagggtca tcccaacgtt acttgagtgt
     4921 gcaaaacctt gagaaaaagt catcattctg ccaccagtca atccttcatt aacgtcccaa
     4981 tctcgaatcg tggcatctgc aatttttaac tcactactat taaagacttc tcctcctgga
     5041 aaggtattat tatcaatagc agcagccaac atcatcactt tcatagtgga acctggctca
     5101 tagttacttt ggtaaaggat atcacgccaa acaaagtcct ctgtaatgcc ttcttttgta
     5161 tctgcatcaa aggtcggtcg ttgcgttgtt gccagaattt cccctgtttt agcactgacc
     5221 aaagtcgctg tcatgtactt tccttttacc ttctcttgaa aagcatccat ctgggtttcc
     5281 ataaaggact ggagggggct ggaaatggtt gtataaacat ccttaccgtc catcgttcgt
     5341 tgggaaactt gttctgttcc gggtacaata ttacccagac gatccttttc ataggtaata
     5401 atgccgtctg tccctgcaag aatactgttc aaggaactct ccattccaga ggttcccagc
     5461 aagctcttgc ttccatcttc attttcatgg agctgagcta ggccgataaa actagaagca
     5521 aattgtccgt ttgggtaact acgattggga ctggttgtaa aatcaatccc cttgacctct
     5581 gcagcttcca attctttttt gatagacatc atattggcat aggtaatccc atttcccttt
     5641 gctccaaagg aaacttgctt gagattaggt tgcgagagtt gctctcttac ataggattct
     5701 tccatgtcca aatacttatg aaagacctct gcaaccttgt taaattgtgt tttttctacg
     5761 taaagaatct tacccgttgc tgacttatag ttctcatcaa tgaccgcata gacattatag
     5821 gaggttgcat cctcagcaat cgggactcca tttcggtcat aaatagtccc acgtttggca
     5881 ggaactgtac gggtggtttg atgaactttc ttagcttcct tcgctaaatc tgttccaaag
     5941 cgagtgcctg tcccaataat gaccgcaaaa ttgactaaaa aaatggcaaa aacaaagaca
     6001 gataataaac tcagactttt tccaactctg cgtctgtttt cagccggcga tttccgattt
     6061 ttggtcgcat aacggattac tctttttgtc cacttcatat cttactccgc tattctaata
     6121 ttttcattgt ttaattgcaa atcgtgtgaa ttggcaattt ctttcaaacg ttctgcacgt
     6181 aatagttcat tgacctcttg cttggcatcg tccaattcgg ttttcttttc ctctatctgc
     6241 gcattgattt ttgtcaaatc attctgcact tgcaagagct tggtctgcat aaaaataata
     6301 ctaatggcta caataagagt ggttacagca atggaaaagt aaaaagcttt ttccacacgc
     6361 gaaaaccgtt taagttgcat ctgtagtatt tgacctgttt tttccatttt ttctgccatc
     6421 tttttccctc ttacttgtga atttttctgg ccacgcgcaa cttggctgag tgcgagcggt
     6481 tattggcttc taactcttcc gcacttggca agattggctt acgggacacc aattccatct
     6541 tgggcttgag atcatctggg atgaaaggca agccttttgg aacttcaact gttgaagctt
     6601 ccttgaacaa ttgcttggtc aagcggtctt ctaaggaatg aaaggtaatc actgaaattc
     6661 taccatccag agccaacata tccatagcct gctggatgga ctcatctgcc gcccccagtt
     6721 catcattgac ttcaattcga atagcctgga aaatctgctt agcaggatgc cccttcttct
     6781 tgagttcctt ggcaggtttg accaacttga taatctctgc taactcagtc gttgtctcaa
     6841 tcggcttcac ttcacgcgct tgctcaatct tacgcgcaat ctgtttagag aatttgtcct
     6901 ctccatactt gaagaaaata cgaaccaagt catgatagtc ataattgttc accacttcat
     6961 acgctgtcag actagcatcc tgattcatcc gcatgtccag tggcgcatcc tttttataag
     7021 aaaaaccacg ctcacgctgg tctaattgag gactagacac tcccaagtca taacaaattc
     7081 catcaatttc ctgaacacca gcttcgcgca aacgtgcctg taaatgacgg aagttgtcct
     7141 tgataaaggt caccattccc ttctcaatgt aaggtgccaa gcgtttttgc gcattgtcaa
     7201 tggcattctg atcctggtca aaggcataga gatggccttt ttcacttaat ttacttaata
     7261 aatactcgct atggcctgct ccacccaaag tcgcatcaac gtagataccg tcaggtttta
     7321 cgtcaagcat atcaatcgtt tcgtggagta agaccgttac atgatgaaat tcttttgtca
     7381 tatcttatct attttaccac aaatccgacc agcttgcact tgtcagacaa ataggacaag
     7441 gagactacta tttcttcaaa aaatatgtca aaaatacttg acactttatg tttaaaagac
     7501 tataatatag tcaaatatat acgacataaa tcagaaagga gaccagatga atcgtgtgaa
     7561 agaatttcgc aaggaattgg gtatttccca gctcgaactc gccaaggata tcggtgtctc
     7621 gagacagact atcaatatga ttgaaaacga caagtacaat ccaaccttgg aactctgtct
     7681 caatctcgcc cgcagcctcc aaactgacct caacagtctc ttttgggagg aagattttta
     7741 aaaaaggagc aaactcatga aaaaagaaac cttcactgaa aaactgatca aacgcacata
     7801 cggtatttct ggtccccttg acgaatacaa acggcgtgag gccgatagta ttgggaacca
     7861 agtctttatc gtcctctttt atctgatgat tttcggaaat attattccac tccttctggc
     7921 ctataaatac cctcaagaag tggctctaat ctatcctcct ctgattttag tgattgccct
     7981 catcgcttct ggctatgtca cctaccaaat gaaaaaaaca ggcatcacag tcattgaacc
     8041 agatatgctg aatgagaaag aaagcaagca actacactac cctggtctga aagcaggttt
     8101 gttctttggt ctatggatgt tttttataac tcctcttctc agtatactca tagatgaagg
     8161 tcaggactat tttcattctc ttctcactat aagaaatggt gtatcaagta ttctcggttc
     8221 tattttcttc ggagcgagca tacagttcct catctcccgt cgcattgcaa aaactaagaa
     8281 aaatcaagat gaggattagg aggaaactca tgaaaaaaga aaagaaacag ttacgttatc
     8341 ctggtctgaa agcaggttcg atttatggaa cagtgatatt ttttataatt ccactcattg
     8401 ataccctaac aagtgaaaat ccaaatttta tcagttctct tctaaataca aaacatattt
     8461 ttaaaactat actgggagct ttctttttcg gagtgatgat acatattgtc gactcacttc
     8521 gtattgcaag agctaaaaaa gaccaggatt aggaggtgcc ttatgaaatc actagctaga
     8581 ctactgatca ttcatgtttt tatcagtatt ttccttttct tcgcccttac ttcaggagct
     8641 atttctcata cagttttact actcctactc ctctttcttc ctgcgctcaa taaaggactt
     8701 gagaaaatac aatcaaaacg gatacctgtc ctcaacgcag ccctcttctt tctcctcata
     8761 tcctttccac aacttttaac caaccctgtc caatggaaat tttcaatatt cctagtcgta
     8821 accatcattt caagtttggc ctacttctat aacttttatc aagtagttaa agaagtagat
     8881 caaaaacagt tgatttagga gcttgctccc ataatttctc attttatagg caaataatcc
     8941 cacctagctt gttctaggtg ggattatttt atttcttaac agagattgca agtcattttc
     9001 cttcatttta gaaagtcgac tctttccaat tcacactaca atccaagact tgttgccttt
     9061 cttcttgatt gcgaccttca atctggtcaa tcaaaatctt tgtagcctgt tgtccttcct
     9121 caaaggaggg ctgaaccagc gtcgaaacac ttggagaaga aaagcaagtc cactccgtat
     9181 tgtcaaaacc aatcaaccca acttgcggca agttataatt caactctttg ataacggtaa
     9241 agactagagg tagggcccaa cagttaggga taaataccag agttttttca tcgggatcga
     9301 tttctttttg taaaaattct ttaatttgtt ccaaattcgt atgcttatct tcaatggtta
     9361 gactggcgtg acgcatatta gcatctgtta aagcatccac aaaaccactt gcccgctcaa
     9421 tccgagtact caaacgactc gtatccgctg taatcaagag aaaatgttca taaccttttt
     9481 cgatacagga ctgggtcatg tcataaacgg catcatagtt attggtttta acccagctag
     9541 tccggtgttc atagagctga ctatcaaaaa agaccatttt cttctttttc tcatcgatga
     9601 tacgagaata ttttcggaaa ttagaggtcg gctgaataat aaagccgtct actcccaaga
     9661 gaagcatgct ttcaatatac cggtcctcac tctcttggct gtaattacta tttcctatca
     9721 ttacctggta gccattctgg ctggcgatat cctcaattcc cttaacaatt tggtttgaga
     9781 aactgttggt aatatcacca atcaaaacac cgattaattt tgttcgtttg gagtttaagc
     9841 tacgcgcaac aatgctcggt ttgtaatttg tttcatgaat aactttttca atcttttcac
     9901 gtgtctcttg ggacattttt tcatatttcc cgtttaggta aaatgacacg gttgttttcg
     9961 aggtctgagc catttccgca atgtctttta tggtcagttt cttctccaat ctaaaacacc
    10021 tcctactaat acttattata acatattcac tattcaaaat gcctatttct agcactgcaa
    10081 gcgaatcatt ctctcattta ttttatcata tactagacat tttccacgca tttttgttcc
    10141 gtcaacgagg cacaattttt cacctacacg aatatcctcg cttaataaca gaatcagctt
    10201 gtctgtttcg tcatttttca cttcaaaagc cagagcattt tccacttcac gatcatctgt
    10261 ctggtagaca gaatgacgga tgatttcaaa actctcatgc gcaatgatgg tataatccag
    10321 catctgattc tcaaagaatt ggcgcttggt gagtttgcta cttctttcaa gctcattgta
    10381 tttaggagaa atgatggtat cttccaaatc aaaatcaact tcactccata gtctcaactg
    10441 attgattttc ccatcttgat aggtcacatc cttgtcaagg ataaactgag tcaacgcctc
    10501 atgctgacct tgacacctga tgtcatctac caagagccag acatcctcta ccaacatgag
    10561 gatttttctc ttgtgaagat aaggcaaatc aggttctgct gaccaataag ccccttcaat
    10621 ataatgcact ccctcccttt ctttatggtg acaaaacagg gagtgaggat agtattcata
    10681 ttcccaggat cccgtgattc tttccggagc tttcccatct acaatgcagg tcgaatgact
    10741 ccaagcactc tttaagagat aacgttcata tatctcccga taagaataac gcccagcatc
    10801 tatgaaaata ggttggcctt gatactgtaa gcaaaaacta ttctcgtcac tatggctatg
    10861 ggcacttcct agcggaccat ttttgaaaaa tagataacga tgttcatcct taatgcagac
    10921 atgtccagag tcttcaaaga tcatggactt aggctgccaa gctctccttt caaattcctg
    10981 cagtcgcttg accttttctc gccccaggaa caagaggcta agcaaatcga ctttaacatc
    11041 cagaccgtta agaaggtctt ccttgttcaa aaccacagca gacagactca aaatttctgt
    11101 cgtttctgta gaatcgctat caccaaaagc caaagttcgt ccatctaagc ctgtcatcat
    11161 ttgaatatag gtcgccatct tttccagcaa ctctcggtaa ctatcttgca agtctggaag
    11221 caagagacac aaatccagca aggctttata aacctctaca tgatagagaa tcgactgttc
    11281 aaactggctt ccatctccta aaatctgtgt ctcaatttgc tgtttcaact cctctgaagc
    11341 aaaatggtaa gcttcttcta gatccatctt atctgaaaag aaataataga tagcaagcat
    11401 cggaattgtt tgtaaaatcc cccagttact aagggtgtac ttggcgcgat agtggctttt
    11461 cataaagtca atctgctttt ctagactgac caaaattttc tctagttctt tctcctctag
    11521 caagtcaaat ttcaagagga gcaagagtag tttcaaccaa gtaaaggaac gaatacccgt
    11581 atccaaggtt ctagtcatca aggattgagg agaaaattct ctcacctgct caatccaatc
    11641 aaatagaaag aacttgcact tttgaatata gctcttatct ccttctacca cataccctat
    11701 cataaactgc aagagatatt cttgtcgatt gagcatataa gcccattctg gatcatcttc
    11761 aaatacttga tcccatacca tcggctggat ttgatggatt tttgaacaag gctccatatc
    11821 ccaaggacta tcaaacataa aacgattgtc catcaagcgt tcaagggaac gcttgacttt
    11881 ctcatagtct tttgaatagt gggacaagat ataatcacga cattggtttc catcgattct
    11941 ttcaaaaaat tgtcttcttt cttctttcat tatctattac cagaaaaaga actacttaaa
    12001 aagcagttct tttgtctttc ccattacact ttccttttct acatagatga ccacaccttt
    12061 tgcaatctgc aaggagacca agtcatcttt gatagaaatg atttttccat gaattccaga
    12121 caataacaac acttcatcac cagatgttaa agaagctaaa tactcttgtc gttgctccat
    12181 ctgtttgcga agcaactttt gctgacgaat agaatgaaag cttgacagta aaaggggact
    12241 cactgccaag acaatcacta ttccataaaa caatgttgta tccattaagc tataatctta
    12301 agccagcttc cgataattcc gatgataact gttaaaataa cgagtttata tgttgtccat
    12361 ttcttttctt tgatcaagta gtaaactaaa agtgtaaata gggctggtag aagagctgga
    12421 gcaaccttat caagcattcc ctgaatactt acgatacttt gtttagcgtc tgctttaact
    12481 tcccctgcag caaaggtaat cggcaccata atcttaacag atgtcgctgc caaaccagca
    12541 attacggtta caccgataat attggcaata cgagaaatcg ttgccatctg ctcgcttagt
    12601 ttatcaatca cagttgttcc tagtttgtat ccatacagac cagttgacaa tttaatcgct
    12661 gttaaaatcg tattcatcgc aaggaagaac aagattggac cgacaaccaa gccttcttga
    12721 gcaaacgaag ctgcgatggt tgagaacaat ggagctaaac agaattgaga aagagaatcc
    12781 ccaatacctg ccaatggtcc catcaaggcc atcttgatgc tacgtgtttc ttttgttggt
    12841 cgaccatttt ccaacattac aagatgcaag ctggtaataa aaggcaggaa gtgtgggttg
    12901 gtattataga attcacagtt ttcttccaag gcttggtaga aaccttcctg atcctctcca
    12961 tagtgttttt tcaaagcagg atacatcaca ttggcatatc ccaacccttg atagttacta
    13021 tagttaaatc cattttgaca aaagaatgcc cgcaaagacg ttttaagata atcacgtttt
    13081 gttaatttgt tagatccagt catcgtgtgc ttcctcctct accacatgat ccgctgtttt
    13141 tggcttgtta taaaattcaa tcaaagcaaa gatagtacct acaattgcaa taccaattgt
    13201 tgggatgttt agataagctg cacaaacata tcccaacaag acaaagggaa tcaactcttt
    13261 cttagccatc actgacaaga tcatcgcaaa accgatagct gggagcattt taccagcaac
    13321 tgtcaaacct gtaagtaata ccggtggaat gtagtctacg agtttcaaca aggtatccat
    13381 tgaaagggca cccaagcaac ccaaggtaaa tccaataaag gcaaacaacc aaattgttgc
    13441 atttagagtg aacttaaatt tcttcaaatt atggtttttc aagtgcttca tcgctgtttc
    13501 aggcgcacca gcacgtacag tgtaggcgaa agtttgtaag aattgaatcg ccacagcaat
    13561 cggagtagag agggcaagag ccgcttctgg actgacttta ccagcactag tgatagccat
    13621 caaggtacca aagataccag gtccgattgg gtttggtgga acagtacctc cagcacctac
    13681 accgaatccc atataagcca attcaccaat agctcccatt gcaagagcag taggcagatc
    13741 acctagaata attccgacac caaatgacag aacaatacat ctattggtgt aaattcctaa
    13801 cagcattcca ctaaaacaga aagctgtcca taatccaatt aaaatcgctt gaaatacatt
    13861 aatcgacata acgatctcct cttttaaata tagtccataa tgttgacttg aacagctcca
    13921 tcatttcctg ttggagttgt tttcgtatta aatgttactt gatgagtttg gctcaattcc
    13981 ttgagggctg ccttgtcctc ttcacccagg aagatggagc gtgtcacttg ctctttacca
    14041 ggggcattgt gaatgttccc aatattgatt tctttgatag ggacaccacc ttctaccaag
    14101 gttaaagcgt cctttacatc ctttacaacg ataaagatcg tttgagcagg attagccttg
    14161 tgaatgatat caatcacctt ttgcaaaggg aagaaacgca tggcaactga gtctggcaca
    14221 actgttttca tcagagtttg ttgcatcttg tccgtgctta cttcgtcatt ggcaacaatg
    14281 accgtattac aacctaggta ttttacccaa agttgtcctt gtccatgaat caaccgttca
    14341 tcgatacggg tcataataat atttggcatt gtcatatttc tcctcctacc aatataggtt
    14401 ccagtctttg tagaaacgga taagggcttc taggtaatag tagtcacccc agatattgcc
    14461 ttcatccact cctttacctg aatgccatga gtacacaccg tggaggagac ttgtcccacc
    14521 aggggtaaat tgatcatttg cataatgttc gatcaaggaa cgaagcatgg catgcatagc
    14581 atgtttataa atatctttgt cagcatccac ctctgggaga tgttttagca tttcatgaat
    14641 cccacagacg gcgatagctg ttgctgaaga atctcgtgat tgatcactac catcattaaa
    14701 aatcaaatcc caataggaca catgatcttt tggcagacga ttcaagaaat aattggtcac
    14761 acccttaaac aagtcaaagc aggactcgtc ttttaagtga cgataagtca aaggaatacc
    14821 atagactccc catgattgac cacgtgccca gcatgaatca tcactatacc cttgtctcgt
    14881 tacaccttta aagggttgac ctgtctcagg atcaaaatag aaggtgtgga aggacgaagc
    14941 gtcatcacgg attacattat tagctgaagc atagaaatga ctttctgcaa tatcgtagta
    15001 tttttgatca cctgtttctt gataagcaaa gaataagagt tggatattga gcaagcagtc
    15061 gataatcaaa cggtaatgct ctttcttgcc caagtctccc caagcttgaa taaaaccacc
    15121 tttttcttga tagcgttcaa tcaacttatc tgcagctttc aaggttgctt ctctagcctc
    15181 tccatctcca tttatcttat attcagccat acaagacggt gtgtacaaga agccgagatc
    15241 atggtgatcc aattctactc tcttattgac acgatccagg aaagaaagaa catttttatg
    15301 agcgatgttt ttaaatgcat cctgttgact gtattcataa gccaaccaca gttctcctgt
    15361 ccagaaacca ttggtccatt ccgtgttatc catgattgga tagacattat caaaggtagc
    15421 tggcgtcggg aaatcttcct tgaaatagtc aaggttgagt tctaactgcc gaataacctt
    15481 atcgattgcc tggccgactt cttctttcgt cagaagtggt acttctaaga agcgctcagg
    15541 cgattttatt ttttcaatcg taaccttttt tatcattctt ttacccttta aatacccgat
    15601 tcgaaatctt cttcctcttc ctctgcatcc gttgaaaaca attctttacc atttacgact
    15661 ccgccctggg ccgccactac tgatttatta acaacctcat caaagctatg agccattcta
    15721 gcaaagactg cttccattaa catggctaag ttcaaacccg agagaacatt cattgttttg
    15781 gctggatttt ctcccattat ggtagaagaa accttgaatg gcgatcctcc caagagatca
    15841 cttaggatta aaacttcttc ttcatttgaa attgcaagta agattttttg cttgagttca
    15901 tctgctgaca ttccttccac aaagtcaatc gcctccacat tttcttgatt gcctgcaatc
    15961 aattgtaaag aactataaat ccctgtagca aaatgtccat gccctacaag tacaattttc
    16021 atcttttctc ctttcttgtt taggtttacc ggtttacttt atattcccca ttgtaatcct
    16081 ttatttttat cttgtcaata gttttttaaa aaaaaatatt agcgctttca ttatcttttt
    16141 aaccatttta ataggcagaa atttcaaaat cttccgtttt tttaggaaaa caaacctttt
//
LOCUS       DAVKMU010000044        15591 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKMU010000044 DAVKMU010000000
VERSION     DAVKMU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.044"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            16..537
                     /locus_tag="VW343_001635"
     CDS             16..537
                     /locus_tag="VW343_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcin A-M57"
                     /protein_id="HEU9756162.1"
                     /translation="MKFKKIVTSALVVLSLFSIMSPSIASVRVFADDVTTTEVSEIST
                     QEQKQIDDVANVLEQMFRNGVNEKNFTEYVYKNFSQKDIALAENELETNINNPYDRVP
                     WDEMGGCIAGKIRDEFFAMINVSLIVKYAQKKAWSELAKVVLRFVKANGLKTNIYIIA
                     GQLAIWAVQCGLE"
     gene            1021..1752
                     /gene="budA"
                     /locus_tag="VW343_001636"
     CDS             1021..1752
                     /gene="budA"
                     /locus_tag="VW343_001636"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9756163.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            1762..1968
                     /locus_tag="VW343_001637"
     CDS             1762..1968
                     /locus_tag="VW343_001637"
                     /inference="COORDINATES: protein motif:HMM:NF021592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9756164.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLDLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALL"
     gene            2005..2514
                     /locus_tag="VW343_001638"
     CDS             2005..2514
                     /locus_tag="VW343_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9756165.1"
                     /translation="MVAFFEETARLVFFKWLEKKRSLEKADALAYGLGHGGLELIFLG
                     LTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQSLSVWQIYLLGFERILALGFQLLL
                     TVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQVGWLTNPVLVEVILALELVLVAYG
                     TKEIFCKKS"
     gene            2626..3531
                     /gene="murB"
                     /locus_tag="VW343_001639"
     CDS             2626..3531
                     /gene="murB"
                     /locus_tag="VW343_001639"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9756166.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            3692..4849
                     /locus_tag="VW343_001640"
     CDS             3692..4849
                     /locus_tag="VW343_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756167.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            4830..5636
                     /locus_tag="VW343_001641"
     CDS             4830..5636
                     /locus_tag="VW343_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756168.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            5633..6406
                     /locus_tag="VW343_001642"
     CDS             5633..6406
                     /locus_tag="VW343_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756169.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            6403..7473
                     /locus_tag="VW343_001643"
     CDS             6403..7473
                     /locus_tag="VW343_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9756170.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGSEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            7742..7981
                     /locus_tag="VW343_001644"
     CDS             7742..7981
                     /locus_tag="VW343_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756171.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLNDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            8256..8741
                     /locus_tag="VW343_001645"
     CDS             8256..8741
                     /locus_tag="VW343_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9756172.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAAIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            8763..11381
                     /gene="alaS"
                     /locus_tag="VW343_001646"
     CDS             8763..11381
                     /gene="alaS"
                     /locus_tag="VW343_001646"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9756173.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(11471..12925)
                     /locus_tag="VW343_001647"
     CDS             complement(11471..12925)
                     /locus_tag="VW343_001647"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9756174.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            13104..13853
                     /locus_tag="VW343_001648"
     CDS             13104..13853
                     /locus_tag="VW343_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756175.1"
                     /translation="MIEFQNVSKLYGDKKALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            13834..15468
                     /locus_tag="VW343_001649"
     CDS             13834..15468
                     /locus_tag="VW343_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756176.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSLMIAILLVIAFSTSLTAFYNVFYESK
                     DLISYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVDPFSTEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
BASE COUNT         4340 a         3047 c         3457 g         4747 t
ORIGIN      
        1 aaaggagtgt tttttatgaa atttaaaaaa attgtaactt cagctcttgt tgtattatca
       61 ctattttcta ttatgtctcc ttctatagct tctgttagag tatttgctga tgatgtaact
      121 actacggaag tttctgaaat tagtacccaa gaacaaaaac aaatcgatga tgtagccaat
      181 gttcttgaac aaatgtttag aaatggagtt aatgaaaaaa attttactga atatgtttat
      241 aaaaactttt ctcaaaaaga tattgctctt gcggaaaatg aattagaaac taatattaat
      301 aatccctatg acagagtccc ttgggatgag atgggaggat gtatagcagg aaaaatacga
      361 gatgaatttt ttgctatgat caatgtttcg ctaattgtta agtatgcgca gaaaaaggct
      421 tggtcagaat tagctaaagt ggtactaaga tttgttaaag ctaatggact taaaacaaat
      481 atttatatca ttgctggtca gttggctatt tgggcagttc agtgtggtct ggaataatgc
      541 gtgataaaaa ctttttaata aaagatggga ttattagctt tttgattgga tttgtatatg
      601 catatttagt gttacaaaaa tttatccttt ggaaaatcct tttgtttgca tttatttttg
      661 caattattaa tacaatattt atgtttacta ggaacaaaaa ataaataatg atttagttaa
      721 ataaaaagcg acccctaggg aagtgtgttc cttaggggtt gttgtgctag tgcaagtttt
      781 gtttctgtca aaatctcagg ctacccgtcg gaaatcgtcc agactcgata ccatttttca
      841 gataggaaac gacctctttg tacttgcttt gtttcttcat tccagacctc ccttgatttt
      901 gttacattgt accctgtttt tccttcttgg caatgtctag agcgtttctc tcgttcacat
      961 ctaggggcaa atgtggtata cttaggagta agatttatag aataacagac aagaaagtga
     1021 atggataaga acgtgcagga accagtgaaa ttatttcaat acaatactct tggagccttg
     1081 atggctggcc tttatggtgg tactatgaca gtaggagaat tgctagaaca tggtgacctt
     1141 ggtttgggaa ccttggattc cattgatggg gaattgattg tcttggatgg caaggcttat
     1201 caggccaaag ggtcaggaga ccagccagaa attgtggaag tgtcaccaga tgcccttatt
     1261 ccttacgctg cagtggtacc gcatcaggca gaggtcattt tccgccagcg ctttgagatg
     1321 acagacaagg aattgaaaga acgaattgag tcttattatg atggggaaaa tcttttccgc
     1381 tctatcaaga ttcgggggga attttcacat atgcatgtgc gtatgattcc taagtcaaca
     1441 cccgatacca agtttgctga tgtcgcaacc catcaaccgg aatatagtcg tgacaatgtt
     1501 gcggggacta ttgttggttt ctggacgcct gagattttcc atggggtcag tgtggcaggc
     1561 taccatctgc acttcatatc agatgatttg accttcggtg gacatgtcat ggattttgtc
     1621 atcaaggaag gcattatcga ggtgggagca gttgaccagt tggaccaacg tttcccagtc
     1681 caagaccgtc aatacttgtt tgctaagttc aatgttgacg agatgaaaaa agatattgaa
     1741 aaggcagaat aggagaagaa aatgaccatt catatcatta ttaccatgtt gctgttgcta
     1801 gcttttctga taggaagcat ttggtttgcc aaaaagaaat accagattaa tctagctgtc
     1861 ttggacttgg gtgcagtcgc tttctttgtc tcttcgcaga ttttggaaaa actggtgcat
     1921 atcttgattt tgcatcctca aaaagacggt agtattgccc tcttgtaagt tcatccgctt
     1981 atctatatta tctatggcct agccatggta gcattttttg aggaaacggc tcgtcttgtt
     2041 ttcttcaaat ggttggagaa aaagagaagt ttggaaaagg cagacgcctt ggcctatgga
     2101 ttggggcatg gtggcttgga gttgattttt ctaggcctta ctagtttact caatctttac
     2161 attgttcttt cggcagttca aactcagaat ccacaggtta tgcaattact gtctgaaaat
     2221 atgttaaaaa ccattcagtc actttcggtc tggcagattt atttgcttgg ttttgagcga
     2281 atcttggcgc ttggtttcca attacttttg acagtttggg tttatcaagc tgttcgccag
     2341 aagaaatgga tttatctcct aacagcctat ggcttgcatg ccttctttga tctggcacca
     2401 tctcttttcc aagtaggctg gttgacaaat ccagtcttgg ttgaagtgat tctagcactg
     2461 gagctcgttc tggtcgccta tggaaccaag gaaatctttt gtaaaaaatc ataaaaagag
     2521 gggaacctct ttttcttatg caaaatccaa acaaggtatt tttatggtcg tcaaatgtct
     2581 ctaaaaatgg tataatggaa tgaattttgt aaaaggaaga gtgacatgtc tgtaagagaa
     2641 aaaatgcttg aaatcttaga aggaattgat atccgttttc aggaaccctt gcatagctat
     2701 agttatacaa aagtaggtgg agaggctgat tatttggtct ttccacgaaa tcgttttgag
     2761 ttggctcgcc ttgtgaaatt tgccaaccaa gaaaatatcc cttggatggt tcttggcaat
     2821 gcaagcaata tcatcgttcg tgatggtggg attcgtggat ttgtcatctt gtgtgacaag
     2881 ctcaataacg tttctgttga tggctatacc attgaagcag aagctggggc taacttgatt
     2941 gaaacaactc gcattgccct ccgtcatagt ttgactggct ttgagtttgc ttgtggtatt
     3001 ccaggaagcg ttggcggtgc tgtctttatg aatgcgggtg cctatggtgg cgagattgct
     3061 cacatattgc agtcttgtaa ggtcttgacc aaggatggag aaatcgaaac cctgtctgct
     3121 aaagacttgg cttttggtta ccgccattca gctattcagg agtctggtgc agttgtcttg
     3181 tcagttaaat ttgccctagc cccaggaacc catcaggtta tcaagcagga aatggaccgc
     3241 ttgacgcacc tacgtgaact caagcaacct ttggaatacc catcttgtgg ctcggtcttt
     3301 aagcgtccag tcgggcattt tgcaggtcag ttaatttcag aagctggctt gaaaggctat
     3361 cgtatcggtg gcgtagaagt gtcagaaaag catgcaggat ttatgatcaa tgtcgcagat
     3421 ggaacggcca aagactacga ggacttgatc caatcggtta tcgaaaaagt caaggaacac
     3481 tcaggtatta cgcttgaaag agaagtccgg atcttgggtg aaagcaagta ggaagctgta
     3541 tttgaaaagc tgctgctatg tcatggaaag gggggtgaaa gcctatcggt agcgaagatg
     3601 tatgcaggtg gttttactcc ctgcaagagg tagtggggac ctgacagagc cccgatcggt
     3661 taatctatga aaaagaagga atttatgcca attgaaaaaa ccaattatcg aattcaaaaa
     3721 cgtctctaaa gtttttgaag acagcaacac caaggttctc aaagacatca actttgagtt
     3781 ggaagaaggg aaattctaca cccttctagg tgcatctggt tcggggaaat caactatcct
     3841 aaacattatt gcaggtttac tggatgcgac gacaggagat atcatgctag acggtgttcg
     3901 tatcaatgat attccaacca acaagcgcga cgtacatact gtcttccaat cctatgcctt
     3961 gttcccacat atgaatgtgt ttgaaaatgt tgcctttcca cttcgcttgc gcaagattga
     4021 caagaaagaa atcgagcaac gcgtagcgga agttctcaag atggttcagt tggaaggtta
     4081 tgaaaaacgt tccatccgca aactttctgg aggacaacgt cagcgtgtgg ccatcgcccg
     4141 tgctatcatc aaccaacccc gtgtggtctt gttggacgag cctttatcag cgctggactt
     4201 gaaattgaga acagacatgc agtacgaatt gcgtgaatta caacaacgat tgggcattac
     4261 ctttgtcttt gtcactcacg atcaggaaga agctcttgcc atgagtgact ggattttcgt
     4321 tatgaatgat ggcgagattg tccagtctgg aacccctgtg gacatctacg atgagccaat
     4381 caaccacttt gttgccacct ttatcgggga gtcaaacatc ttgccaggta ccatgattga
     4441 ggactacttg gtcgaattta acggcaaacg ctttgaagcg gttgatggtg ggatgaagcc
     4501 aaatgaacct gttgaggtcg ttattcgtcc agaggacttg cgcattaccc ttcctgaaga
     4561 aggcaaactc caagttaagg tcgacaccca gctcttccgt ggagttcatt atgaaattat
     4621 cgcctatgac gaacttggaa atgaatggat gatccactca acccgtaagg ccatcgtggg
     4681 tgaggaaatc ggtctggact ttgaatcaga agacatccac atcatgcgtc tcaatgaaac
     4741 cgaagaagag ttcgatgctc gtatcgaaga atacgtggaa atcgaagagc aagaagcagg
     4801 tctgatcaat gcgatcgagg aggaaagaga tgaagaaaac aagctctaaa ctctttgtag
     4861 tgccctacat gctttggatt gccctctttg tattggcacc cttggtcttg attttcggac
     4921 aatccttttt caacattgaa ggccagttta gtttagaaaa ttataaatct tactttgcgt
     4981 cacaaaactt gacctacctc aaaatgagtt tcaactcagt gctttatgca gggattgtga
     5041 cctttgtgac cttgcttatc agctatccaa cagccctctt tttgacccgt ctcaagcacc
     5101 gtcaactctg gctcatgctg attatccttc ctacctggat caatttgctc cttaaagcct
     5161 atgcttttat cgggattttt ggtcaaaatg gctctattaa ccaattcttg gaatttatcg
     5221 gaattggttc acaacagttg ctttttaccg atttttcctt tatctttgtc gcaagctaca
     5281 tcgagctccc ctttatgatt ttgccgattt tcaatgtctt agacgatatg gataataatc
     5341 tcatcaatgc tagttatgac cttggtgcaa ctaagtggga gaccttccgt catgtcatct
     5401 tccctctatc tatgaacggt gtgcgaagtg gggttcagtc ggtctttatc ccaagtttga
     5461 gtctcttcat gctgacccgt ttgattggtg ggaaccgcgt tatcaccttg gggacggcta
     5521 ttgagcagaa ttttctaacc aatgacaact atggtatggg ttcaaccatc ggtgtgattc
     5581 tcatcctgac catgttcatc accatgtggg tgactaagga aaggagagaa cgatgaaaaa
     5641 atttgccaac ctttatctgg gactggtctt tctggtcctc tacctgccta tcttttactt
     5701 gattggctat gcctttaatg ctggtgatga tatgaatagc tttacaggtt ttagctggac
     5761 tcactttgaa accatgtttg gagatgggag actcatgctg attttggctc agacattttt
     5821 cttggccttc ctatcagcct tgatagcgac cattatcggg acttttggtg ccatttacat
     5881 ctaccagtct cgtaagaaat accaagaagc ctttctatca ctcaataata tcctcatggt
     5941 tgcgcctgac gttatgattg gtgctagctt cttgattctc tttacccaac tcaagttttc
     6001 acttggcttt ttgaccgttc tatctagtca cgtggccttc tccattccta tcgtggtctt
     6061 gatggtcttg cctcgactca aggaaatgaa tggcgacatg attcatgcgg cctatgactt
     6121 gggagctagt caatttcaga tgttcaagga aatcatgctt ccttacctga ctccgtctat
     6181 cattactggt tatttcatgg ccttcaccta ttcgttagat gactttgccg tgaccttctt
     6241 tgtaacagga aatggctttt caaccctatc agtcgagatt tactctcgtg ctcgcaaggg
     6301 gatttcctta gaaatcaatg ccctgtctgc tctagtcttt ctctttagta ttatcctagt
     6361 tgtaggttat tactttatct ctcgtgagaa ggaggagcaa gcatgaaaaa aatctattca
     6421 tttttagcag gaattgcagc gattatcctt gtcttgtggg gaattgcgac tcatttagat
     6481 agtaaaatca atagtcgaga tagtcaaaaa ttggttatct ataactgggg agactatatc
     6541 gatcctgaac tcttgactca gtttacagaa gaaacaggaa ttcaagttca gtacgagact
     6601 tttgactcca acgaagccat gtacactaag ataaagcagg gtggaacgac ctacgatatt
     6661 gccattccaa gtgaatacat gattaacaag atgaaggacg aagacctctt ggttccgctt
     6721 gattattcaa aaattgaagg aatcgaaaat atcggatcag agtttctcaa ccagtccttt
     6781 gacccaggta ataaattctc catcccttac ttctggggaa ccttaggaat tgtctacaac
     6841 gaaaccatgg tagatgaagc gcctgagcat tgggatgacc tttggaagcc ggagtataag
     6901 aattctatca tgctctttga tggggcgcgt gaggtgctgg gactaggact caattccctc
     6961 ggctacagcc tcaactccaa ggatctgcag cagttggaag agacagtgga taagctctac
     7021 aaactgactc caaatatcaa ggctatcgtt gcggacgaga tgaagggcta tatgattcag
     7081 aataatgttg caatcggcgt gaccttctct ggtgaagcca gccaaatgtt agaaaaaaat
     7141 gaaaatctac gttatgtggt accgacagag gccagcaatc tttggtttga caatatggtc
     7201 attcccaaaa cagttaaaaa ccaaaactca gcctatgcct ttatcaactt tatgttgaaa
     7261 cctgaaaatg ctctccaaaa tgcggagtat gtcggctatt caacaccaaa cctaccagcg
     7321 aaggaattgc tcccagagga aacaaaggaa gataaggcct tctatcccga tgttgaaacc
     7381 atgaaacacc tagaagttta tgagaaattt gaccataaat ggacagggaa atatagcgac
     7441 ctcttcctac agtttaaaat gtatcggaag taggagataa acgtaagaag acgaatcagt
     7501 caagctggtt cgtttttaag gaatagaagt cagtttttag agccctatct ttttctattc
     7561 tagttgttta tttgcttatt tgcttatttg cttatttgct tgcctagtag tatactaagc
     7621 ctatactctt caaaccatgt cagcgttgcc ttgccgtact caagtacagc ttgaggctag
     7681 cttcctagtt tgctttttga tttttattga gtattaacct tacagaaagc ggcaacaaat
     7741 aatgaaaatg tctacttttt tcaaaaaaag tttctggcca acctttatga ttgttaacca
     7801 aactgctata ctattccatt taaatgatgg cttggatcga caatacctaa caactgagtc
     7861 catatattgg gtgataggca cctttatatt tggaaatatt ctagtagctg tgtttagtaa
     7921 tatgaaaatt tgggataaaa agaaaaatgg cagtaagaaa aaatatatcc tgaaaaagta
     7981 atcagagatg tagggaattg gtctaattca acggagatca tttccagttg acgcaagctt
     8041 aaaaaaacgt tataataaga aagttgagaa ttgattttca cttgtcttag tccagagaaa
     8101 tggcggtgct gcgagccatc taagctagga agtcatgcta ctcaatcata caattgcata
     8161 agaacaagag aatgacaagt tcattgaatg aaggtggtac cgcggttttt cgcccttcgt
     8221 gatatgagct tgtcttttga tttttggagg tgttgatgaa aacatttctt gtcaaacaaa
     8281 agtttcgtct tggaggcgaa cgcttcgcta tcaaggatga caggggagaa attgcctatc
     8341 aggtggaggg atcatttttt aagattccca aaacttttac catctatgat gcggctggtg
     8401 aacaagtcag tcagatcagt aaagaaatct tgaccttgct tcctcgtttt gagattcagc
     8461 ttcgggatgg ctcgagtttt gtcattcgta agaagttgac cttctggcga gataagtatg
     8521 agtttgataa tctaggtctt cgtatcgagg gcaatatctg ggatttgaat ttcaaattgc
     8581 tggatgatcg cgatcagttg attgcggcaa ttaagaagga actcttccat ctgacctcta
     8641 cctataccgt aacggttctt gaagacgctt atgcagacct agtcatttcc ctctgtgtcg
     8701 cgattgacta tgtggaaatg ctggaaagcc aatcacatta aacaagtgaa taaggagaca
     8761 atatgaaaca actatctagt gcacaagtac gccaaatgtg gcttgatttc tgggcgacca
     8821 aaggtcactc agtagaacca tcagtaagtt tggttcctgt aaatgaccca actcttttgt
     8881 ggatcaactc tggggtagca acccttaaga aatactttga cgggaccatt atccctgaaa
     8941 atccacgtat taccaatgcc caaaaggcta tccgtaccaa cgacatcgaa aacgtaggga
     9001 agactgcgcg tcaccatacc atgtttgaaa tgctggggaa cttctctatc ggggattact
     9061 tccgtgacga agctatcact tgggcttatg agcttttgac aagccctgaa tggtttgatt
     9121 tccctgctga aaaactttac atgacctact atccagacga taaagattct tacaaccgct
     9181 ggattgaagt gggagtggat ccaagtcact tgattccaat tgaggacaac ttctgggaaa
     9241 tcggtgcggg accttctgga ccagatacag aaatcttctt tgaccgtggg gaagcctttg
     9301 acccagaaaa tatcggtctt cgcctgcttg cagaagatat tgaaaacgac cgttatattg
     9361 aaatctggaa catcgttttg tcacaattta acgcagaccc tgctgttcct cgtagcgaat
     9421 acaaggaatt gccacataag aacattgata cgggcgctgg tttggagcgt ttggtggccg
     9481 ttatccaagg ggctaagacc aactttgaaa cggacctctt catgccgatt atccgtgaag
     9541 tcgagaaatt gtctggtaag gtttatgacc aagatggcga caacatgagc ttcaaggtta
     9601 tcgcagacca cattcgttct ctttcatttg ctatcggtga tggtgccctt ccaggaaatg
     9661 aaggtcgtgg ttatgttctt cgtcgtcttc tccgtcgtgc ttctatgcat ggtcaaaaat
     9721 tgggtatcaa cgagcctttc ctttacaaat tggttccaac tgttggaaaa atcatggaaa
     9781 gctactaccc agaagtgctt gagaaacgtg actttattga gaaaatcgtt aagagcgaag
     9841 aagaatcatt tgcccgtacc cttcactcag gtcaacactt tgcccaaggc attgtggcag
     9901 acttgaaaga aaaaggtcaa tctgtcattg ctggtcaaga tgtctttaaa ctctatgata
     9961 cttatgggtt cccagttgaa ttgactgaag aaatcgctga agaagctggt atgactgtag
    10021 accgtgaagg ttttgaagca gccatgaaag aacagcaaga acgcgcgcgt gcgtcagctg
    10081 tcaagggtgg ctcaatgggt atgcaaaatg aaactcttca aaacatcact gtagaaagtg
    10141 tcttcaacta caatactagc caattgtctt ctaaattggt agctatcgtt gctgacaatg
    10201 cagaagtagg agctgtttca gaaggaactg cctctcttat ctttgcggaa acgtcatttt
    10261 atgctgaaat gggtggacag gtagctgact acggacaaat cttggatgag tcaggtaaga
    10321 ttgtggctac tgtgaccaat gttcagaaag ccccaaatgg tcaagccctt catacagttg
    10381 aagtccttgc accgcttgcc ttgaaccaag aatatacctt ggcaattgat agcaatcgcc
    10441 gtcaccgtgt catgaaaaac cacactgcga ctcatttgct tcacgctgcc cttcacaata
    10501 tccttggaaa ccacgcaaca caggcaggat ctcttaacga agttgaattc cttcgctttg
    10561 actttaccca cttccaagca gtaactgctg aggaacttcg tgcgattgaa cagcaagtta
    10621 acgagaaaat ttgggaagca cttgaagtga agacagttga aacggatatt gacactgcta
    10681 aagaaatggg agctatggcc ctctttggtg agaaatacgg caaggaagtt cgtgtcgtga
    10741 ctatcggtga ctactctatt gaactttgtg gtggtactca tgttggcaac acttctgaga
    10801 ttggtctctt caaaattgtc aaagaagaag gaatcggttc aggaactcgc cgtatcttgg
    10861 cagtgactgg taaggaagcc tttgaagcct atcgtgaaca agaggatgct cttaaagctg
    10921 tcgcagcaac cttgaaagca cctcaagtca aggaagtacc tcacaaggta gaaggacttc
    10981 aagaacaact tcgtcaactt caaaaagaaa atgctgagtt gaaagaaaaa gccgcagctg
    11041 cagccgcagg cgatatcttc aaagatgtta aggaagtcaa cggtcatcgt tacattgcta
    11101 gtcaagtgtc tgtatccgat gcaggtgccc ttcgtacctt tgcagataac tggaaacaaa
    11161 aagactactc tgatcttctt gtcctagttg ccgctatcgg tgacaaagtc aatgtccttg
    11221 tagcaagcaa gacaaaagac cttcatgcag gaaaccttgt caaagaatta gcaccaatca
    11281 tcgatggacg tggtggtggt aaaccagaca tggccatggc aggaggaagc aaccaaccta
    11341 agatccaaga attgttggat gcagtagcag gtaaattgta atcaagtaaa gatctatcca
    11401 tttgggtagg tctttttgtg aatacaaaaa agccaaatcc gcttggacct agcttggtta
    11461 ttatgagcta ttagatggta ttgactgccc agacacttac ggatctagct gagacaggga
    11521 attgtccata accttcctca tcaattgtaa cttgaccttg gtggttacca agtaaatcta
    11581 caaaggtttg attagtccat tcttgaccga caaacattga cttgctgttt tcttggtcat
    11641 ttgagataag gactgcgatt ggggattgat tttcagcacc tgaacgtacc caaccgatac
    11701 agttagcatg gtcaaagtag tcattttgtt ctccataggc caaatctttt cggatggcta
    11761 ggaggcggtc aaggatttct ttgaaatctt cttgagcata ctggcctgaa atcccatagt
    11821 agtctccgta aaagacacat ggaaggccgt cttggcgtaa caaaatgagg gcataggctg
    11881 ctggcttgaa ccattcttca acggtagact caagggcttg tcctcgttgg gtatcgtggt
    11941 tgtcgacaaa agtcacagcc ttgtcaggct tgagttcaac caggctatct gtgaaaatgc
    12001 cacgaaggtc atagtttgcg ccagcttgac tggcttcaaa gagattctga tggagacgaa
    12061 catcgacaag gtcaaagtgt tcttccgttt tttcgagata gtccagattg gcttccttgt
    12121 ctgggttcca aaattcacca aaaacataga aatcgtcacc gtatttttcc ttcatatcgc
    12181 ggatgaagtt gcgcataaag aaagagtcaa tatgcttaac ggcatccaaa cggaaaccag
    12241 ctacaccagt cgtttccatg aaccaatcag cccagtcata gatgttttgg atgacttcag
    12301 gatgtttaaa gtctaggtcg gcatacatga ggtagtcgta gtttccgttt tcgttatcga
    12361 ccaattcctc gttagcccag cccttgttgt ccccttggat cagataaatc ccagacttac
    12421 ggcgtttggc atcatagtct gtacctgtga agtggtacca gtgccagtgg aagtcattgt
    12481 aggtatcttg gcgaccatcg aaggtaaaac tagtccagcc attgatggtg aagggttctc
    12541 caagttcaac tgtacggtct acaggatcca cttcaataac ttgaaaggct tccctgtgat
    12601 cagcagcagc cttgtggttg agaaccacat cggccatagg ttgaattccc tgcgctttaa
    12661 gggcttgaat ggcttgaaga tagtcttctt tgaaaccata cttggtgcgg acagtccctt
    12721 tttggttgaa ctctcctaag tcaaataagt catagacccc atagccgaca tctttttcgt
    12781 tggttgcctt gaaggctggt ggcatccaga cgtgactgat ccccagatga gctaggtgtg
    12841 gagcattttc agccagacgc gtccagtgtt gaccgtcgtg gggcagatac cattcaaagt
    12901 attgcatgag tgtttgattt tgcataatgt ttcctcttac ttgtcaatct tgttctttat
    12961 tctatcataa agtatctatt agcgcaaacg tttgcgtaag atagaaggaa actattttaa
    13021 ctacttaaaa taaagtattg atttttttgt atcaaagtgc tagtatagcg atatataata
    13081 cagataaaga taggagttgt cccatgattg aatttcaaaa tgttagtaag ttgtatggtg
    13141 ataaaaaggc cttgagtaat ctcaatttgc agattgaaaa tggagagatt atgggcttga
    13201 ttggtcataa tggggctgga aaatcgacca ctataaaatc cctagtcagt atcatttcac
    13261 ccagcagtgg tcgtattttg gtagacggtc aggagttatc ggaaaatcgc ttggctatta
    13321 aacgaaagat tggctacgta gcagactcgc ctgacttatt tttacgctta acggccaatg
    13381 aattttggga attgatcgcc tcatcctatg atctgagtag atctgacttg gaggctagtc
    13441 tagctaggct attgaacgtt tttgattttg ctgaaaatcg ctatcaggtt attgaaactc
    13501 tttctcacgg aatgcgtcag aaagtctttg tcatcggagc actcttgtct gatcccgata
    13561 tttgggtctt ggatgaaccc ttgactggtt tggatcccca ggctgccttt gatttgaaac
    13621 agatgatgaa ggaacatgca caaaaaggga agacagtctt gttttcaact catgtcctag
    13681 aggtggcaga gcaagtctgt gatcggattg ccattttgaa aaaggggcat ttgatttatt
    13741 gtggtagtgt agaggacttg agaaaagatt acccagacca gtctttggaa agtatctacc
    13801 ttagtcttgc tggtagaaaa gaggaggttg cggatgcgtc tcaaggtcat taaaaaatta
    13861 gttgatatca atatccttta ttcatctcaa gaagctaatc tggctaatct acgaaagaag
    13921 caggctaaga atcctgggaa aaaagtaaat gtttccgcta gagtcttaag ttcttacatt
    13981 ttttccagtc tcttgatgat catctgtttt agtaatatag ccattcattt tccttttgag
    14041 gaaataccta tttattttag cttgatgatt gctattttac tagtgattgc cttttcaact
    14101 tctttaacgg ccttttacaa tgtcttttat gagagtaagg atttgatatc gtataggccc
    14161 tatgccttta aagaatcaga gattataatt gctaaaggac tgtctgtcct tttgccagct
    14221 ctaactggaa ttgtaccaat cctagcttat tttctggtcc tctacattag gctagctcct
    14281 tctctatggc tgggtttgcc tttgatgcta ctgtccttga ccttattatt tgtctctgtg
    14341 gctctagtga tggtagtggc agtgcatttc ttggctcaaa ctagggtctt cagaaagtat
    14401 cagtctattt tttcgaatgt gatgattggg ataggagttc tcataccttt aatatttatc
    14461 ttctttcttc agtcgacttt tggaagtatt gttgacaaag ttagagacat tccatttctc
    14521 ctttatcctc ttcatatctt ttacaaaata gcagtggatc ctttttcgac agaagccata
    14581 ttgggtctgc tcgcttggat aggactaact ctcttcctgc tttatctgac caaaaagaag
    14641 gtccttcctc gtttttatga cgtgatcctg cttaatagtg aggagaaggt caaaaaagaa
    14701 cgtcgcagca aggagagaat ttcaaccact aaaaagggat ttttccgcat ggttttacgc
    14761 taccacctca ccctcttggg acagggtact ggcgtggtta cagtgctttt tacaagtgct
    14821 ttctttcctt atctcatgat gatcggtctg atttccaaaa tccgagattc tcagatagtt
    14881 ccagacattc atcctccata ctggttaccc ttgttttttg taggagtgtt tatagcagtt
    14941 gtcaataaca atatcaccag cctgcattca attgccttgt ccttggagag ggaaaatgtt
    15001 gattttctta agagtttacc ctttgacttt gctcgttatg tgaaagtgaa attttggatt
    15061 atatatgctg tccagtcctt tttaccagtt ctgactttgc ttggtctttc tctatatcta
    15121 ggcttgccca tcatttcgat gatttacctt attgtggcat ggatccttgc cagtgtcatc
    15181 ctttcttgcc accattactt taaggacgtt aaaaatctgt caaccaattg gagtagcatt
    15241 acggacctgg tgaatcgttc aaatggcata gtcgccatag ttttattgtt tatttatagt
    15301 gcaatcctga tggcccttgt aattgggagc atattcttgg ttcagtctct ctcccctatc
    15361 cttgccatca acttgggagt aggagctctt atcgtcttgc ttgctcttgc tatttttggc
    15421 tatcattatt acctgtcacg catattggca gaaatagaaa aaagatgagc tggttggttg
    15481 cttgcttgat aaattcaatt cgacaaacgc ttttatagtt tgttagaatg tagttgtaaa
    15541 tgattaagat tggtaatcaa aaatataggg aggaatgttt tgaataagaa a
//
LOCUS       DAVKMU010000045        15572 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKMU010000045 DAVKMU010000000
VERSION     DAVKMU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.045"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            87..884
                     /locus_tag="VW343_001650"
     CDS             87..884
                     /locus_tag="VW343_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9756177.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSIFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGSLIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1032..1154
                     /locus_tag="VW343_001651"
     CDS             1032..1154
                     /locus_tag="VW343_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU9756178.1"
                     /translation="MTRDFKFETLQLHAGQVVTPATESRAVPIYQTTSFVFDDT"
     gene            1176..2291
                     /locus_tag="VW343_001652"
     CDS             1176..2291
                     /locus_tag="VW343_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU9756179.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDIDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGSSCHNLSYIRDVGAATFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETLSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGDEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPNQIRLSIGLENVEDLIEDLRLALEKI"
     gene            2387..3661
                     /locus_tag="VW343_001653"
     CDS             2387..3661
                     /locus_tag="VW343_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9756180.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3671..4549
                     /gene="truB"
                     /locus_tag="VW343_001654"
     CDS             3671..4549
                     /gene="truB"
                     /locus_tag="VW343_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9756181.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4790..5092
                     /locus_tag="VW343_001655"
     CDS             4790..5092
                     /locus_tag="VW343_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9756182.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5200..5838
                     /gene="udk"
                     /locus_tag="VW343_001656"
     CDS             5200..5838
                     /gene="udk"
                     /locus_tag="VW343_001656"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9756183.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5966..7306
                     /locus_tag="VW343_001657"
     CDS             5966..7306
                     /locus_tag="VW343_001657"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9756184.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7284..7496
                     /locus_tag="VW343_001658"
     CDS             7284..7496
                     /locus_tag="VW343_001658"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9756185.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7493..8368
                     /locus_tag="VW343_001659"
     CDS             7493..8368
                     /locus_tag="VW343_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9756186.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8361..9176
                     /locus_tag="VW343_001660"
     CDS             8361..9176
                     /locus_tag="VW343_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9756187.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDVKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9169..9600
                     /locus_tag="VW343_001661"
     CDS             9169..9600
                     /locus_tag="VW343_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9756188.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9607..11274
                     /gene="recN"
                     /locus_tag="VW343_001662"
     CDS             9607..11274
                     /gene="recN"
                     /locus_tag="VW343_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9756189.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGVPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLHLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11276..12004
                     /locus_tag="VW343_001663"
     CDS             11276..12004
                     /locus_tag="VW343_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9756190.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12083..13906
                     /gene="lepA"
                     /locus_tag="VW343_001664"
     CDS             12083..13906
                     /gene="lepA"
                     /locus_tag="VW343_001664"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9756191.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14200..14280)
                     /locus_tag="VW343_001665"
                     /pseudo
     CDS             complement(14200..14280)
                     /locus_tag="VW343_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14454..14929
                     /locus_tag="VW343_001666"
                     /pseudo
     CDS             14454..14929
                     /locus_tag="VW343_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            14967..15269
                     /locus_tag="VW343_001667"
     CDS             14967..15269
                     /locus_tag="VW343_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9756192.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            15277..15572
                     /locus_tag="VW343_001668"
                     /pseudo
     CDS             15277..15572
                     /locus_tag="VW343_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         4658 a         2826 c         3637 g         4451 t
ORIGIN      
        1 ataaactttt atgaaataca gaaaacgctt cctaaaacct aaagtttgtg atataattat
       61 caagaaaaaa gatttaggag tttattatga tttcttcaga atttatctca aagattgaat
      121 ttgcttgcaa taagaaagaa agtctttata gtcaaagcaa atttaagtat gcgattcgtt
      181 cgatattcgc aggtgcattt ttaaccttca gtactgctgc aggtgcagtt ggggctgact
      241 tgattaataa aattgcacca ggtagtggac gcttcctctt tccattcgtt tttgcttggg
      301 gcttggccta cattgttttt ttgaatgccg agttggtcac ttcaaacatg atgttcttga
      361 ctgctggtag tttcttaaaa aaaatctctt ggagaaaaac agctgagatt ttactatact
      421 gtaccttgtt caaccttatc ggatccttga tagcagggtg gggctttgct cattcggcag
      481 cctatgcgaa tctgacacac gatagtttca tctcaggtgt tgttgagatg aagttaggcc
      541 gctcaaatga attggtcttg cttgaggcga ttttggcaaa tattttcgta aatattgcga
      601 ttctgtcatt tattttggtc aaagatggtg gtgccaaact ttggcttgtg ttgtcagcta
      661 tttacatgtt tgtattctta acaaacgagc acattgcggc gaactttgct tctttcgcga
      721 ttgtgaaatt cagtgttgct gcggattcaa ttgccaactt cggtgttgga aatatgcttc
      781 gccactgggg tgtgactttc atcggaaact ttatcggagg aggcctcttg atgggtcttc
      841 catatgcctt cctcaataaa aacgaagata cttatgtaga ttaagaaaat gagcacgatt
      901 gagtcgtgct tttttcattt tcaaaataag gtaatagcta tttcttatat caaaatatag
      961 aaaactgata tttgtaaact ataactcaag gtgctacaat atccttaata aaataatatg
     1021 gaggtcacct tatgactcgt gattttaaat ttgaaactct acaactacat gctggtcaag
     1081 ttgtgactcc agctactgag tctcgtgcag tgccgattta tcaaacaaca tcttttgttt
     1141 ttgatgacac gtaggaaggt gctgatctgt ttgccttgag gaaaccaggg aacatttata
     1201 ctcgtatcac caatcctaca acagctgccc ttgaaggtgg tgttgaagcg ctagcaacag
     1261 catcaggtat gactgcagtg acttatacga ttttggcgct tgcccatgct ggtgaccatg
     1321 tagtggctgc ttcgactatt tacggtggaa ccttcaatct tttgaaagaa ccccttcctc
     1381 gttatggtat cacaacaacc tttgtcgata ttgataattt ggaggaagta gaagcagcta
     1441 tcaaagacaa taccaagctt gtcttgattg aaaccttggg taactccttg attaatattc
     1501 cagacctgga aaaactggca gagattgctc ataaacatca aatcccactt gtgtcagaca
     1561 atacttttgc aacaccttat ttgattaacg tcttctctca tggcgttgac attgccattc
     1621 actctgcgac taagtttatc ggtgggcatg gtacaactat tggaggaata attgtcgata
     1681 gtggtcgttt tgactggacg gcttcaggga aattccctca atttgttgac gagggttcaa
     1741 gctgccacaa tttgagctat attcgtgatg tgggtgcagc aacctttatt atagctgttc
     1801 gagttcaatt gcttcgtgat acaggtgcag ccttgtcacc attcaatgct ttcctcttgc
     1861 tacaaagact tgaaactctt tcacttcgtg tggaacgcca tgtacaaaat gctgagacaa
     1921 ttgttgattt tcttgtcaac catcctaagg tagaaaaggt aaattatcca aaacttgcag
     1981 atagtcctta tcatgccttg gctgagaaat acttgccaaa aggtgtcggt tcaatcttta
     2041 ccttccacgt caaaggtgac gaggaagaag cacgcaaggt cattgataat ttagaaatct
     2101 tttctgacct tgcaaacgcg gcagatgcta aatcgcttgt tgtccatcca gcaacaacca
     2161 ctcacggtca attgtcagaa aaagacctag aagcagcagg tgtcacacca aaccaaattc
     2221 gtctgtcaat cggtcttgaa aatgtagaag atttgattga agacttacgc ttggccttgg
     2281 aaaaaattta aagtaaaaga agataaacag tgggttttga ctcactgttt ttgattttcc
     2341 ctcaggcatg atataatggt tacagaagtc tagaaagagg aatgtaatga acgaaatcaa
     2401 atgtcccaac tgtggggaag tctttacagt aaatgaaagc cagtatgctg aacttttgtc
     2461 ccaagtgaga acggcagagt ttgataagga actacacgat aggatgaggc aggaactggc
     2521 cttggctgag caaaaggcta tgaatgagca acagactaaa ctggctcaga aggatcaaga
     2581 aattgcgcaa ttacagagtc agatccaaaa ctttgataca gaaaaagaat tggccaagaa
     2641 agaggttgaa cagacaagcc atgaggctct cttggctaag gacaaggaag tacagctctt
     2701 agaaaatcag ttggctacct tgcgtttgga gcatgaaaat caactacaaa agaccctttc
     2761 tgacctagaa aaagaacggg atcaggttaa aaaccaacta cttttgcagg aaaaggaaaa
     2821 tgaattatct ttggcttctg ttaagcaaaa ctacgaagcc cagctcaagg cagctagtga
     2881 acaagtcgag ttttataaga attttaaggc tcaacaatct acaaaagcga ttggggaaag
     2941 cctagaacag tatgcagaga gtgagtttaa caaggttcgt agtttcgcct ttccaaatgc
     3001 ttactttgag aaggataaca aggtctcttc gcgtgggtct aaaggggact ttatcttccg
     3061 tgagtgtgat gaaaatggag ttgaaatcat ttctatcatg tttgagatga aaaacgaagc
     3121 ggacggaaca gagaagaagc acaagaatgc agatttttac aaggaattgg acaaggaccg
     3181 tcgggagaag aactgtgagt atgccgtttt ggtgaccatg cttgaggctg ataatgacta
     3241 ctttaacaca gggattgttg acgtcagtca cgagtatgaa aaaatgtatg ttgttcgtcc
     3301 tcaattcttt atccaattga ttggtctctt acgtaatgcg gcgctaaatt ccctaaaata
     3361 caagcaggag ttggccttgg ttcgcgagca aaatattgac attacgcatt ttgaggaaga
     3421 tttggatgcc tttaagctag cttttgctaa gaactataat tcagcttcga ctaactttgg
     3481 aaaagctatt gatgaaatcg acaaggccat caaacgcatg gaagaggtta agaaattcct
     3541 gaccacatct gaaaaccaac tccgtttagc taacaacaaa ttggaagatg tctctgttaa
     3601 aaaattgacc cggaaaaatc caacaatgaa agcgaagttc gaagcactga agggggagta
     3661 gaaagcaaaa atgaacggta ttattaactt aaaaaaggaa gcaggaatga cctcgcatga
     3721 tgcggttttt aaactgcgta agattttggg aaccaagaaa attggtcatg gtggaacctt
     3781 ggatccggat gtggtgggtg ttttgccgat tgcggttggc aaggcgacac gcatggtcga
     3841 gtttatgcag gacgagggta agatctatga gggggaaatc actctgggct attccacgaa
     3901 gactgaggat gctagtgggg aagtggtcgc agaaacccct gttttgtctc tcttggatga
     3961 aaagcttgtt gatgaagcga ttgctagctt gactgggcct attactcaga ttccccctat
     4021 gtattcggca gttaaggtta atggtcgcaa gctctatgag tatgcgcgtg ctggtcagga
     4081 agtggagcgt ccagaacgtc aggtgaccat ttatcaattt gagcgaacaa gtccgatttc
     4141 ttatgatggc caacttgccc gattcacttt tcgtgtaaaa tgcagtaaag ggacgtacat
     4201 ccgtactttg tcagttgatt tgggtgaaaa gcttggttat gcggctcata tgtcccattt
     4261 gactcgtact agtgctgctg gcttacaatt agaagacgct cttgccttgg aggaaattgc
     4321 tgaaaaagta gaggctgggc aattagattt tctccatcct ttagagattg ggacaggtga
     4381 ccttgtcaaa gttttcctaa ctccagaaga ggctacagaa gttcgctttg gtcgttttat
     4441 tgagctagac caaacggaca aagaactggc tgcctttgaa gatgataaat tgttagccat
     4501 tctagaaaaa cggggcaatc tctataagcc aaggaaggtt tttagctaga tcgtttagga
     4561 ataaaaatcg ggtgatagat aacaattgct tgataaaacc ccatactaat agtagaatgg
     4621 ttttgggaat tataatattc caattgttgc gagttgtagg tactcaaata atctatatag
     4681 aaatttagag gtgtgaaatg aagcaattta aaattctttc agataaatat ttagagtcca
     4741 ttacaggttc tgatgggaac ttaggcccag gatttggtgt gataattcca tgatgcgaaa
     4801 tgagtttcga gaaagggtgg agcaacttct tcaacaaaaa gaaataaatg aaaatagtga
     4861 gttgagtcac ctgtttcgtc ttgctataca aaatttagac agaaatgaaa aataccaatc
     4921 ggtcatggcc aatttgagtc aagggttgtc actttacctc atgacgcatc attaccaggc
     4981 acctaagtct gtcattgatt ttggtttatg gattgccaaa gctcctagcc aggaaagagg
     5041 gagactggct ttcttgcaga tgcttgctca aaccctacag ggctttcgtt aaaactgtcg
     5101 aacatcagag ggaagactct ggtgtttttt gctgatgaga ctataaattt tgtaaaaaat
     5161 gtgatagaat agacgacgga taaaataacg gaggatagca tgcaaaatag accaatcatt
     5221 attggagtga caggtggttc tggtggtggt aaaaccagtg tttcaagagc cattttatcg
     5281 cattttcctg atgaaaagat ttccatgatt gagcatgatt catactacaa ggatcagtct
     5341 catttgacct ttgaagagcg tgtcaaaacc aactatgacc atccttttgc ctttgataca
     5401 gacttgatga tcgagcagat taaggaattg ttggcagggc gtccggtgga catcccgacc
     5461 tacgactata cagagcatac acggagtagc aagacctatc gtcaggaacc tcaagatgtc
     5521 tttatcgttg agggtatttt ggtcttggag gacaagcgtc tgcgcgattt gatggatatc
     5581 aagatttttg tggatacgga tgacgatgtg cgcattattc gtcgtatcaa gcgtgatatg
     5641 gaggagcgtg gccgtagcct tgatagcgtt attaaccagt acttaggtgt ggtcaaacca
     5701 atgtaccacc agtttatcga gtcaactaag cgttatgctg atatcgtcat tcctgaaggg
     5761 gttagcaata ccgtggctat cgacctgttg acgaccaaga ttgcaaagat tttggaagaa
     5821 gctcgaaaca gcaaataatc acatgtggag gccttgcctc ctttttctat ttttccctca
     5881 taatggtaca taaatggaga tttttaggca tatttttggt ataataatac ccatgaaaga
     5941 gcaagtaaat gaatagtagg tggagatgga aaagtattta tcggtaacaa ctttgaccaa
     6001 gtatctgaaa atgaaattcg ataaagaccc atacttggaa cgggtctatt taactggtca
     6061 agtttccaac tttcgtaaac gacctactca ccaatatttc tccctaaagg atgaccatgc
     6121 agttattcaa gcgaccatct ggtctgggat ttatcagaaa ttagggtttg acctggaaga
     6181 aggaatgaag atcaatgtga ttgggcgtgt acaggtctat gaaccaagtg gtagctactc
     6241 catcatcatt gaaaaagctg agcctgatgg ggttggggcg cttgcgattc agtttgaaca
     6301 acttaagaaa aaattgacag aagaaggcct gtttcaagaa cgcttcaagc aagctctgcc
     6361 ccaattttct aagagaattg gtgtagtaac cagccgtagt ggagccgtta ttcgagatat
     6421 tatcacgacc gtcagcaggc gattcccagg tgttgacatc cttctttatc cgaccaaggt
     6481 tcaaggtgaa ggggctgcgg aggaaattgc tagaaatatt gcgcgtgcta atcaacggga
     6541 cgatttggat ttgctcatta ttggtcgtgg aggtggttct attgaggatc tctgggcctt
     6601 taacgaagaa attgtggtac gagctatttt tgaatctcgt ttgccagtta tctctagtgt
     6661 ggggcatgag acggatgtga ccttggcaga ttttgtggca gatcgacgcg ctgcaacgcc
     6721 aacagcggcg gctgaactgg caacacctgt gaccaagttg gatgtattag ctcatttgca
     6781 aaatcaggaa aaacggatgg taacggcagt ccgaaatgtt ctatctaaga aacaagaggc
     6841 tttgaaaaaa tgcagtcagt ctgttatctt tagacaacct gagcgcttgt atgacggtta
     6901 tttgcaacgc ttggaccaac tgcaactgcg tttgaaacaa agtttgcgaa ctcggatttc
     6961 tgataacaaa caattagttc aagcaagaac tcatcaatta gtacaattat cacctgttac
     7021 caaaatccaa cgctatcaag accgtttagg acagttggac aagctcttag gtagccaaat
     7081 ggcgttagtt tatgacgcca aggttgctga ggccaagcga ctttcggaag ctttgctcat
     7141 gttggatact agccgaatcg tggcgcgtgg ttatgctatt gtcaaaaaag aagagtccgt
     7201 tgtagattcg gttgagagtt tgaagaaaaa agaccaagta acgcttttga tgcgagatgg
     7261 tcaagtagaa ttagaggtta aagatgtcaa aacaaaagaa atttgaggaa aatctagcag
     7321 aactggaaac cattgtccaa agtttggaaa atggtgaaat tgctctggaa gatgcgatta
     7381 ctgcctttca aaagggcatg gtcttgtcaa aagagctcca agctacgctg gacaaggctg
     7441 aaaagacctt ggtcaaggtc atgcaagaag acggaacaga aagtgatttt gaatgaaaaa
     7501 gcaagaaaaa ttagctcttg tcgagtcggc tttggaagat ttttatggag accagcagtt
     7561 tgcctctagt ttacgggagt ctgttctcta ttctattcat gctggtggca agcgtattcg
     7621 gccttttctc ttgttagaag ttctggaagc cttgcaggtt accatcaaac ctgctcacgc
     7681 gcaggtagct actgccttag agatgattca tacagggagc ttgattcacg atgaccttcc
     7741 tgctatggat gatgacgatt atcgaagagg gcggttaacc aatcacaaga aattcggtga
     7801 agctatggcc attttggctg gagatgcttt attcttagac ccatatgcct tgattgcgca
     7861 ggcagatttg ccaagtcaga tcaaggtgga cttgattgcc aacttatccc ttgcttcagg
     7921 tagtctgggt atggtggcag ggcaagtttt ggatatggag ggcgaacacc agcacttgtc
     7981 tctggaagaa cttcagacta ttcatgccaa taagactggg aagttactag cctatccctt
     8041 ccaagcggca gctattatag ctgaattgtc acctgaaatg caggtgaagc tgaaaactgt
     8101 gggtgaattg attggacttg cttttcaagt cagagatgat gtactggatg tgacagctag
     8161 ttttgaggaa atcggcaaga cacctcaaaa ggatctgcag gcagaaaaat caacctatcc
     8221 tgccttgttg ggcttggaag agtccattgc cttttgtaac caaaccctgg atcaagctaa
     8281 tgaaaaatta gaagaaattg cccagcagct tccctttgaa acagaatcga ttgtaagtgt
     8341 agtagaaagt ttgagaatca atggctaagg aaagagtgga tgtactagct tataaacagg
     8401 ggttgtttga aacgagagag caggccaagc gaggtgtgat ggctggccta gtcgtagcag
     8461 tccttaatgg agaacggttt gacaagccag gagagaaaat tccagatgac accgaattaa
     8521 aactcaaggg ggagaaactc aagtatgtca gccgtggtgg tttgaaactg gaaaaggcct
     8581 tgcaggtctt tgatttgtcg gtggatggcg cgactacgat tgatatcggg gcctctactg
     8641 gaggttttac cgatgtcatg ctacagaata gtgccaagtt ggtctttgca gtcgatgttg
     8701 ggaccaatca gttggcttgg aaattacgcc aagacccacg agttgtcagc atggagcagt
     8761 tcaatttccg ctatgctgaa aagactgatt tcgagcagga gccgagcttt gccagtattg
     8821 atgtgagttt catttccctt agtctgattt tgccagcctt gcaccgtgtc ttggctgatc
     8881 aaggtcaggt ggtagcactt gtcaaacctc agtttgaggc aggacgtgag cagattggga
     8941 aaaatggaat tattcgagat gttaagattc atcagaatgt ccttgaatct gtaacagcta
     9001 tggcagtaga ggcaggtttt tcagtccttg gcttggactt ttctcccatc caaggtggac
     9061 atggaaatat tgaattttta gcgtatttga aaaaagaaaa gtcagcaagc aatcagattc
     9121 ttgctgagat taaagaagca gtagagaggg cgcatagtca atttaaaaat gaataaaaaa
     9181 gaaagacttg aaaaaattag aagattggtg acagattatc aaatcggcac gcaagaagaa
     9241 attgtagaac atttgaaaga agcaggtatc actgccactc aggcgacggt atcccgagat
     9301 atcaaagagt taggtattgt caaaattcct ttgagagaca acacctatgt ctacgaatta
     9361 ccaaaatcaa tcgtaaaaag tctgcaactg gctgaagaca atatcgaatc ggctgaattg
     9421 atggataaga tgatcaatct ccaagttatt ccaggaaata cggcttttgt aaaagctcag
     9481 ttaatcgaga cttttgcaga caagattttt agctgtttga ctgatgatag ctcgatttta
     9541 gtcattgcca gaagtggaag tctagcagag gaaatctttg aacaagtaaa aaattggtag
     9601 gtctatatgt tacttgaaat ttcgataaaa aactttgcca ttattgaggc tatttccctc
     9661 aattttgaaa aggggatgac tgtcctgact ggtgaaacgg gtgcagggaa gtcgattatc
     9721 attgatgcca tgaatatgat gttgggagct cgtgcgacga cagatgttat tcgtcatggt
     9781 gtaccaaagg cagagattga ggggcttttc tcagttgaga atagtcgtct tttacaggaa
     9841 atttttgatg agcaaggttt agaattaggt gatgaaatta tcatccgtcg ggaaatcttg
     9901 caaaatggtc gtagtatcag ccgtgtaaat ggtcagatgg ttaatctgtc tgttttgcga
     9961 gctattgggc aacatcttgt agatattcat ggtcagcatg accatgagga gttaatgcgt
    10021 ccccaactgc atatccagat gttggatgaa tttggtgatg ccgctttttg ggacttgaaa
    10081 gaaacctatc aaacgagttt tgatgcctat cggaaaatgc gcaagcaggt tctggaagtc
    10141 aagaaaaacc aacaggaaca caaggcacgt atcgaaatgt tggaatttca aatggcagag
    10201 attgaggcag caaacttgca ggctggagaa gacttggctc tcaatcaaga gcgagataaa
    10261 ctcctcaacc acaaaaatat tgcggataca ctgaccaatg cctacagtat gttggacaat
    10321 gaagattttt caagtctggc caatgttcgt tcagccatga atgacatgga aagtgtcgaa
    10381 gagtatgacc ctgaataccg tgaaatttca agctctctgt ctgagaccta ctatgtttta
    10441 gaagacatta gcaaacgttt ggaagctatc attgaggacc ttgattttga tggcaatcgc
    10501 ctcatgcagg ttgagaatcg tttgcacctc cttcatacta ttactcgtaa gtatggtggg
    10561 actgttgatg atgttttgct ttattttgcc aagattacgg aagaatacaa tctcttgaca
    10621 ggcaataatc tttcgtctga ggacatggaa gcagagctta agaagttgga agtcaatctt
    10681 gtcaatttgg caggtcaact tgcttctgct cgtcataatt tggctcagca actcgaagct
    10741 gagattaaac aagaactgca agatctttat atggaaaaag cccagtttca ggttcatttt
    10801 agtaagggaa aattcagtcg tgggggaaat gaaatggttg agttttatat ttcaaccaac
    10861 cctggagaag actttaaacc cttggttaag gttgcttctg gaggggaatt atctcgtctc
    10921 atgttagcca ttaagtctgc cttttcacgt aaagaaggca agactagcat tgtctttgat
    10981 gaggtggata cgggagtttc aggtcgtgtt gctcaagcta ttgctcagaa aattcataaa
    11041 attggtcagc atggtcaggt tttggctatc tcccatttgc cacaagtaat tgcgattgca
    11101 gattatcaat tctttattga gaagattagt aatgaccatt caacggtttc gactgttcgt
    11161 ctcttgacag tcgaagagcg agtggaggaa gttgccaaga tgttggcagg tgatgatgtg
    11221 acagaagcag ccctgacgca agccagagaa ttgttgagaa acagggagaa ataagatgac
    11281 agattattat gtaattggag atgttcacgg aaaagctggg atgctggaag accttcttaa
    11341 aacctgggat ggtcagaccc agttgctctt tctaggtgat ttgattgaca gaggtgagga
    11401 tagtcaccgt gttcttgaaa tggtcaagga cttggtggac aatcaagggg ctatctgttt
    11461 gtctggaaac cacgagtata tgtttttgac ttggctagat gacccagaag aaagttatga
    11521 ccattatcgt cgcaatggtg gagatacaac cattaactct atcctaggtc gtcccttgga
    11581 tgcaccagtt gatggagtag aagatgccaa gcgggttgcc gctgaagcgt cagacttggt
    11641 cgaattcatt cgtcaaatgc catttgtagt agagacagac aagtatatat ttgttcacgc
    11701 aggtattgat ttgactttgg acgactggca tgaaacaaca gattataaaa aagtatggct
    11761 tagaaaacca ttccacggag ctgaaaatca tactggaaaa accattgtct tcggtcatac
    11821 accggtctat ggtttgttac agcaagaacg cggtacatct gagctttgga ctacagaaga
    11881 tggcaagatt ggaatggatg gaggagctgt ttatggtggt gtccttcacg ggattgtctt
    11941 tacagaccaa ggaatgacag aacaccactt tatcgaaaat gacggctttg tcgctgaaga
    12001 ttagtactcc tagcagggta tggtcttgtc aaaatatcaa aaacaattta taataaatag
    12061 ataccctgaa aggaagagaa tcatgaactt agaagaattg aaaaaacgac aggggaagat
    12121 ccgtaacttc tctattatcg cccatattga ccacgggaaa tcaactctag cagaccgcat
    12181 tttggaaaaa acagagacgg tttcaagtcg tgaaatgcag gcccagcttt tggatagcat
    12241 ggatctagag cgggaacgtg gaattactat taagttgaat gccatcgagt tgaattacac
    12301 tgcaaaagat ggggaaactt atattttcca cttgattgac acaccagggc acgttgactt
    12361 tacctatgaa gtttcacgtt cgctagctgc ctgtgagggt gctattttgg tggtcgatgc
    12421 ggctcaagga attgaggctc aaactcttgc caacgtttat ctggctttgg ataatgattt
    12481 ggaaatcatg cctatcatta ataaaattga cctgccggct gcagatccgg agagcgtgcg
    12541 tacagagatt gaagatgtga ttggtttgga tgccagtgaa gcagttttgg cttctgccaa
    12601 ggctggtatt gggattgaag aaatcctcga gcaaattgta gaaaaagtac cagcaccaac
    12661 gggtgatgtg acggcgccac ttaaggcctt gattttcgac tctgtttacg atgcttaccg
    12721 tggggttatc ctccaagtgc gtgtcatgga cggagtggtc aaacctggtg ataagattca
    12781 gctcatgagc aatagtaaga cctttgatgt ggccgaagtc ggtattttta caccaaaagc
    12841 ggttggtcgt gatttccttg cgactggtga tgttggttac attgcggcgt ctatcaagac
    12901 ggttcaggat actcgtgtgg gtgataccgt taccttggca accaatcctg cggcagaacc
    12961 attacatggt tataagcaga tgaatcctat ggtctttgcg ggtctctacc caatcgaatc
    13021 aaacaagtac aatgacctgc gtgaagcgct tgaaaaattg caactgaatg atgctagtct
    13081 tcagtttgaa ccagaaacat ctcaggcact tggatttggt ttccgttgtg gatttcttgg
    13141 acttctccat atggatgtta tccaggaacg tttagagcgt gagttcaata ttgatctcat
    13201 catgacagct ccgtctgtta tttacaaagt taatttgacc gacggtgagt ctatggatgt
    13261 gtctaaccca tctgagtttc cagacccaac taagattgcg accattgaag agccttatgt
    13321 caaggcgcaa atcatggtac cacaggagtt cgtcggagca gtaatggagc tagctcagcg
    13381 taagcgtggg gactttgtga ctatggacta tattgatgat aaccgtgtca atgttatcta
    13441 tcaaattcct cttgctgaaa tcgtctttga cttctttgat aaacttaagt cttcgacacg
    13501 tggttatgca agttttgact acgaattgtc agaatatcgc ccatctaagc tagtgaaaat
    13561 ggatatcctt ctcaacggag acaaggtgga tgccctcagc tttatcgttc ataaggactt
    13621 tgcttatgaa cgtgggaaac tcatcgttga taagctcaag aaaatcatcc ctcgccaaca
    13681 atttgaggtt ccaatccaag cggctattgg acacaagatt gtcgctcgta ctgatatcaa
    13741 ggcccttcgt aagaacgtac ttgctaaatg ttatggtggt gacgtttctc gtaagcgtaa
    13801 actccttgaa aaacaaaaag ctggtaagaa acgcatgaaa tccatcggat cagttgaagt
    13861 tccgcaagaa gccttcctca gcgtcttgag tatggatgaa gaataagtta tcagaaatgc
    13921 ctttccttgt tcacaggaaa tttatataag aatcaatagt aaagcaggct tagatggtct
    13981 gcttttttag tttttatact cttcgaaaat ctcttcaaac cacgtcagcg tcgccttacc
    14041 gtatgtatgg ttactgactt cgtcagtttc atctacaatc tcaaaaccat gttttgagct
    14101 gacttcgtca gtttcatcta caacctcaaa accatgtttt gagctgactt cgtcagtttc
    14161 atctacaacc tcaaaaccat gttttgagct gacttcgtca gtttcatcta caacctcaaa
    14221 actgtgtttt gagtaacctg cggctagctt cctagtttgc tctttgattt tcattgagta
    14281 ttagaacaga aaaatgcttg gagtatttgt ttgtgtgttt atttttatat aacaaactat
    14341 aaacacaaca aaaatataaa aaaagagaca aaaaagaaca gaaagtaatt aacaacgatt
    14401 tcatatagtg ttatacttat agcataaagt taatggaaag aagggaaaac cttatggtat
    14461 tagattattt ctttgacaaa aaccttgtgt tttgcttaga agcggataat caagaataac
    14521 tctttgatca ggtagcaagc ttgttggagg aacgagaaat tgtaactcca acttatcgtg
    14581 aagctttgat cacgcgtgaa aagtcatttc caactggttt agatatggaa tttctaggaa
    14641 aggacttgca aatgtaatga ttcctcacac agatattgtt cataatctag ctgagaaagt
    14701 ggtggttgtt cgattagaga aaccggtgac tttccataat atgattgcgc caggtaagga
    14761 agtagaagta tccttgctct tctttatcat caataattca agttcaagtc aaacgaacat
    14821 tcttgctcag ttgatggact ttttcacagg aaatggacat cttgaagacc tatcaaaaat
    14881 ttccgaacca gaaaaacttt atgcttatat agatgaagca atcgcttaat cttgtctatt
    14941 aaaaaataaa atcggaggaa gtctatatga ttaaaattct tgctgcctgc ggtgcaggtg
    15001 ttaactcaag tcaccaaatt aaaagtgctc tagaagaaga actttcaaac cgtggttacg
    15061 atgttcactg tgatgcagtt atggtgaaag atgtaaacga agaccttatg aaaggttacg
    15121 atatctttac accaatcgct gcaacagacc ttggttttga accaggtatc ccagttatcg
    15181 aagctgggcc aatcttattc cgtatcccag caatgagcgt tccagtattt gacaatatta
    15241 gacttcctgc gaaacaaaat atggtatagt agttctatga atgatgaagc aagtaaacaa
    15301 ctaactgatg cacgatttaa gcgtcttgtt ggtgttcagc gtaccacttt tgaagagatg
    15361 ttagctgtat taaaaacagc ttatcaactt aaacacgcaa aaggtggacg aaaacctaaa
    15421 ttaagcctag aagaccttct tatggccact cttcaatagt gcgagaatat cgaacttatg
    15481 aacaaattgc ggctgatttt ggtattcacg aaagcaactt tatccgtcgg agccaatggg
    15541 ttgaagtaac tcttgttcaa agtggtgtta cg
//
LOCUS       DAVKMU010000046        15012 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKMU010000046 DAVKMU010000000
VERSION     DAVKMU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15012
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.046"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..987)
                     /locus_tag="VW343_001669"
     CDS             complement(<1..987)
                     /locus_tag="VW343_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9756193.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIIKKDTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYA
                     FTKGKMSFIAQDFNNLNIITVLKGRTQAVIRNHFLKYDRAVRCRVKIITMDMFSPYYD
                     LARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDS
                     RKLSDKRFYRPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLLFHFQNKKPE"
     gene            1101..1625
                     /locus_tag="VW343_001670"
     CDS             1101..1625
                     /locus_tag="VW343_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9756194.1"
                     /translation="MIEPIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     HEWMLTDIKNFLTKHKAWVIDGNYSWCCYQERMQEADQIIFLNFSPLTCLFRTFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1648..2358)
                     /gene="deoD"
                     /locus_tag="VW343_001671"
     CDS             complement(1648..2358)
                     /gene="deoD"
                     /locus_tag="VW343_001671"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9756195.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(3016..3528)
                     /locus_tag="VW343_001672"
     CDS             complement(3016..3528)
                     /locus_tag="VW343_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9756196.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(3553..4533)
                     /locus_tag="VW343_001673"
                     /pseudo
     CDS             complement(3553..4533)
                     /locus_tag="VW343_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(5009..5818)
                     /locus_tag="VW343_001674"
     CDS             complement(5009..5818)
                     /locus_tag="VW343_001674"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9756197.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(5818..6375)
                     /locus_tag="VW343_001675"
                     /pseudo
     CDS             complement(5818..6375)
                     /locus_tag="VW343_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            complement(6377..7588)
                     /locus_tag="VW343_001676"
     CDS             complement(6377..7588)
                     /locus_tag="VW343_001676"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9756198.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(7602..8285)
                     /gene="rpiA"
                     /locus_tag="VW343_001677"
     CDS             complement(7602..8285)
                     /gene="rpiA"
                     /locus_tag="VW343_001677"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9756199.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(8567..9439)
                     /locus_tag="VW343_001678"
     CDS             complement(8567..9439)
                     /locus_tag="VW343_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9756200.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            complement(9581..10438)
                     /locus_tag="VW343_001679"
     CDS             complement(9581..10438)
                     /locus_tag="VW343_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9756201.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(10592..11326)
                     /locus_tag="VW343_001680"
     CDS             complement(10592..11326)
                     /locus_tag="VW343_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9756202.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESRKVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(11326..12009)
                     /locus_tag="VW343_001681"
     CDS             complement(11326..12009)
                     /locus_tag="VW343_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9756203.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            12199..12429
                     /locus_tag="VW343_001682"
     CDS             12199..12429
                     /locus_tag="VW343_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9756204.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            12658..14916
                     /locus_tag="VW343_001683"
     CDS             12658..14916
                     /locus_tag="VW343_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9756205.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKTEVKSSEKEK"
BASE COUNT         4458 a         3317 c         2769 g         4468 t
ORIGIN      
        1 ctccggtttc ttattctgaa agtgaaacag caagagttga tagagctgat agtggtgttt
       61 caagtcttgt gaatagctca aaagcttgtc taaaatctct ttattggtta agtgcatacg
      121 aaaagtagga cgataaaatc gcttatcact cagtttacgg ctatcctgtt gtatgagctt
      181 ccagtagcgc ttgatagcct tgtattcatg ggattttcga tgaaactgat tcatgatttg
      241 aacacgcaca cgactcatag cacggctaag atgttgtaca atgtgaaagc gatccaacac
      301 gattttagca cacgggaaaa gttgtctagc taaatcatag taaggactaa acatatccat
      361 agtaatgatt ttgacgcgac atcggacggc tctatcatat ttaagaaagt gatttcggat
      421 gacagcttgt gttcttccct taagaacagt gatgatattg agattgttaa aatcttgcgc
      481 aatgaagctc atctttcctt ttgtaaaagc atactcatcc caagacataa tctcaggaag
      541 acaagaaaaa tcatgtttaa agtgaaagtc attgagctta cgaataacag ttgaagttga
      601 gatggaaagc tgatgggcaa tatcagtcat agaaatcttt tcaattaact tttgagcaat
      661 cttttggttg atgatacgag ggatttggtg attcttcttg acgatagaag tttcagcgac
      721 catcattttt gaacagtgat agcacttgaa tcgacgcttt ctaaggagaa ttctagtagg
      781 cataccagtc gtttcaagat aaggaatttt agaaggtttt tgaaagtcat atttcttcaa
      841 ttggtttccg cactcagggc aagatggggc gtcgtagtcc agtttggcga tgatttcctt
      901 gtgtgtatcc ttcttgatga tgtctaaaat ctggatatta gggtctttaa tgtctagtaa
      961 ttttgtgata aaatgtaatt gttccatatg aatctttcta atgagttgtt ttgtcgcttt
     1021 tcattatagg tcatatggga ctttttttct acaataaaat aggctccata atatctatag
     1081 tggatttacc cactacaaat attatagaac caattatcgg atattctggt tctggtaagt
     1141 caactctagc agaaaagtta tctaactact actccattcc aaaactgcac atggacacac
     1201 tccaatttca acctggttgg caagacagtg accacgaatg gatgttaacc gatataaaaa
     1261 actttctcac caagcataaa gcttgggtca tcgatggtaa ttattcttgg tgctgctacc
     1321 aagaacgaat gcaagaagct gaccaaatca tctttctcaa tttttcgcca ttgacctgtc
     1381 tctttagaac ctttaagcgt tatcttaaat accgtggaaa agtcagagaa agtatggcgg
     1441 cagattgccc tgaacgcttt gactgggagt ttatcagatg gattctttgg gatgggcgta
     1501 gcaaaactca aaaagaaaat taccaaaaac tttgccaaga atattcacat aaagtcacta
     1561 ttcttcgaaa tcagagagag ctagatcaat ttctggataa gaaaaggaag tcctacaatt
     1621 cataaagggc ttcctttttg gctataatta ttctgcaatc aaggtttcca aaccaacctt
     1681 catcatatca gtgaaggtat tttgacgttc ttctgcagtt gtgtcttcgt ctggattgac
     1741 caagctatca gagatggtca tgatagctag cgcatcaaca tggtattggg cagcaagata
     1801 gtaaagagct gctgcttcca tttccacagc cttgactccc catttaccaa gctcgatatt
     1861 cttttcaaag taatttgagt aaaagacatc agatgacaaa acgttcccaa cgtgagtagt
     1921 cataccaagt tttttggcga tatggtaggc tttatcaagc aaatcaaagc tagcaatttg
     1981 tggaaaatcg tactgtggcc agtcattacg aacgatgttt gagttggttg cagccgcctg
     2041 cgccaaaact aattcacgaa catgaacctc ttcattcaaa gaacctgcag ttcccacacg
     2101 aatcaatttc ttcacaccgt agtctacgat taactcacgc gcataaatcg aaatagatgg
     2161 cattcccatc ccagttccca tgacagatac acagtgaccc ttgtaagtac cagtgtaacc
     2221 aaacatgtta cgcacttcgt taaaacaaac agcatcatca aggaaattct ccgcaataaa
     2281 cttagcacga agaggatccc caggaagaag aattttatca gcaatttcac cctgctgagc
     2341 agcaatatgg atagacataa tttatgatac aaagagcgag aagaaaacga ctgaaaatta
     2401 ggaacctgac gagaaatcct gatttttcag tcagattatc tattttccga gttttccgct
     2461 cgtgttcaaa tcaaaacaca cgctctacct ttctttattt tatattttat attgagaaag
     2521 ataccaaacc catcaaaaag cgaagggaaa ataggagttg ggcgcagtga gcgatgctcg
     2581 ctagaccaac tatctttttc ccactgcttt tagggtgggg tcaattcctt tctttcttaa
     2641 ttttgattta gaggagagtc gcccgtattc agttcagcga atacagttta cccatccttt
     2701 cgtttttatt tttagaaaag ttttctactc gtgttcaaat tagaacacgc gctctacctt
     2761 tctgtttata ctcttcgaaa atctcttcaa accacgtcaa cgtcgccttg gattatatat
     2821 gtgactgact tcgtcagtct tatctacaac ctcaaagcag tgctttgagc aacctgcggc
     2881 tagtttctta gtttgctctt tgattttcat tgagtattaa ttttgttctt tcacagagag
     2941 ctacctgagt tctattcaag ctcaggtagt actttcttat aaactagaca aactaactgt
     3001 cattgtacca tcagattaca agacatcatc gtcactcacc ttggaattca atgtcgtacc
     3061 ccaatgggta attttacggt ggggttgagc taaaattggt ctgttttcat agattgtttg
     3121 ccatctattc catagtaggc ccgtcttttt ctcaatctta actcgcagat ttctcatatt
     3181 ttctttgatt gggaggttga ggacaaaacc tgcagtctgg ttgcgaccgt ttccttccca
     3241 agaatgacta cgaacaactt ggtttccatc tttatctact gtaacttctt cccaagttat
     3301 ggagtagcgg gcaatgtaag ctccactgtg ttgaattgtc aatgttttat ctttcacagg
     3361 agtctgactg attggttgaa ctggcttaga aacttgtgtc gccgtttcag cattcgtagc
     3421 tataaagcgg aaaacttcaa cttctgcaag actgagggca ttatagcctt ctagcccaat
     3481 ccgaacatag cgcgcttttt tatggttaat cgtaatttgt gctgacacat ctttaggaga
     3541 tattatacgt tttcactcaa tttctttacc agaactgtct aaaagaatga catcaaagtt
     3601 tgccaatcta tcctgggcag tgtctgttcg gttgtaaata ttgatttggc gaatggtttc
     3661 ttctttagcc aaatctactt gccaccaagg cttagattgg aagtttgtat gagtgacaga
     3721 attgtgacca tagttaccat cgactttgcc atccacagct cttctagcat ctcctccgaa
     3781 agctgtcgaa ctttgagtaa cctgttttcc taaggcaata ttttccattg gttcagcgct
     3841 tggttgacta gaagattgag gtagagcctg tttcttaaag aatcgcactt tatccaattt
     3901 cggatcacga taaccatgtt tatccaaggt agcttgttta ccaatattaa agttaggaat
     3961 atcattcatt ggactttcaa aatagccacg agaacggtgt ttaaagcgtg aattacctgt
     4021 aaatgtaaca atatcacgat tattatgaat caagggagtc acaacattgt cattatcaac
     4081 cacataatgc ttaatttttc cactaacagc taatttacga ctttccaaac caacatctca
     4141 gaaaccattc ttagcatccc aaacagttct ggaagtaatc actttaggag cactgaaagt
     4201 tgtcactttc ctcaatacat ggttataaaa atcaggatat ttttggtaag cttcaaccgc
     4261 tgcaatctga gctaggtaac atccaagaga atgacctgtc atatacaact tagtaatact
     4321 tatatccttg gctaattccc caacaacctt gcggatatca tccgcttgag ctggctctat
     4381 tatctctact tttgactctt cccttttgtt ttcaagaact tcacttgctt ccggaagcgt
     4441 ttgaggagct agtcttggct cctctctaag aggagtttct agttttggag catcattttc
     4501 tgactctttc aattcctcct tagaatctag cacacctatt accacttcag atgttacatc
     4561 ttgctttact tcattggctg aaattccagc tggagctaga aatcaaagcc gactacaaca
     4621 gatacaactc cgatacttaa ttttttatgc caaagcgcat aattcgttga ccaattttca
     4681 ttatttctta tctttctttt cgtttattta caagcagact caagcgaaat ctcattgtgc
     4741 tatatatgat aagactgaga aattcagaaa gtcaattcat tctcatctct ccttatcata
     4801 ctagtctact tggctaggaa gctaatgcta gtatcctaga atcttgtctt tcacaatgct
     4861 ggctatcaga tcagacacac ctattcttcc gcttctttca gttataagag caggaagaag
     4921 ctcatagtat tcctctgttt cttctttaat ccggataggc agtcaatcct gaaaaaacag
     4981 aggcactccc ccttttaaat cttttttctt acaattcagc aagaatcgct ttaagcaagc
     5041 ctttgaaatc acctttaaca cgttcagtca cttctacaac ttcttcgtga ttgagttctt
     5101 cttggaaacc ggccgcaaag ttagtgatac atgaaattcc cagaactttc aagccagagt
     5161 gggccgccac gataacttca ggaaccgtag acataccaac tgcatctgct cccagtgtct
     5221 tataggaacg aatttctgct ggtgtttcat aagtcggacc agtaactccg atatagacac
     5281 cttcatcaag cttgatatta agttttttag ccacttcatg ggcagtggca cggtattctg
     5341 gtgtgtaggc cctagacata tctgggaaac gtgggccaaa gtcatccaag ttttcaccca
     5401 tcaatggatt ttgccccgtc atgttgatat ggtctgagat agccatcaag gtaccaggac
     5461 caaatccgat accgccagct gcattggtta caataacacc ttcacatcca agaactttca
     5521 tcacacgaac tgggaaagtc accacttcca gaggattccc ttcatagaaa tggaaacgcc
     5581 cttgaagagc caagaccttg cgacctgcca gttcaccata taccaattta ccagcatgac
     5641 cgactactgt tgaacggccc cagtttggaa tctcagcata gtctactaca actggatttt
     5701 cgatttcttc tgccaattct ccaagtcctg acccaaggat tagaccgaac tcaggggctg
     5761 caattccctt ttctttcagg aaagtagcag tttcatggat tttgtttaaa aatgtcatta
     5821 tgtgtctcct ttattatttt ttagcatttg accaattttg tcaaaggttt tagcattacg
     5881 aatagtaatg tggcggtaga aaggcacctt cagcaagtac ttatgatagg cagtcttaga
     5941 ataggattct tcagaaaatt tcccccagaa aatcccaagt tttctaaaat aaagcacttc
     6001 atctttcagc tctaaacttt caactgtcgc gatgacttgg tccacatcca aaccctcagt
     6061 gtaaaagaga aaatcttttc gtgccaagtc tctgctccac caagctggta gattttcaag
     6121 ttccgcctca aagtcctcta gactcagtaa agaaaagctc tgaataaatg gataatggac
     6181 tgcaaagaaa gtctctagct tttaaaccaa ttgggctttg gaatctatcg aagtaaagaa
     6241 aatattgcca ctgttgatgt agctctcaac cttttccagt cccaagtttg tcaattcttg
     6301 acgaagctcc gccataacga ccttattctt actaccaaca ttgatacctc tcaccagcaa
     6361 agcatagcgc gtcatcttat accaatttat ctaagaaact ttccccaatc atagcagttt
     6421 ccacaccaaa gttatcggca acagtcgctg aaatatctgc aaaatgtcct actggaatga
     6481 gaccatttcc tttaaaggca gggctatatg ccaacaatgg aatatattcc cgagtgtgat
     6541 ccgtccctgc atacgttggg tcatttccat ggtccgcagt aatcaagaga aggtcattct
     6601 ctctcatagc tgcgataatt tcaggtaagc gttcatcaaa ctcatgcaaa caatcacggt
     6661 aaccgtgagc attacgacga tggccgtaaa gggcatcaaa gtcaactagg tttgtgaatg
     6721 agaatccttt ttcaaactca gcaagtccca tagtcttcaa tagtgtatca attccatgac
     6781 tatttgactt gttgtgaccc atgtcatggt tgataccagc accgttaaag atatcgttga
     6841 ttttacccac agcataagta tcgataccag cctcattcaa tttatccaaa acagttgggg
     6901 aaaatggaga tacagccaag tcacgacggt ttgccgtacg agtgaagtta cctggttcac
     6961 ctacataagg gcgagcaatg atgcgaccaa gaagggcagg acgctcaagg gtaatcgaac
     7021 gagcgtattc acagatacgg tacaattcat ccaaaggaat aatgtcttcg tgggcagcaa
     7081 tctgcaaaac agggtcagct gaagtataga taatcaactc tccagtttcc atctgacgtg
     7141 gtccaaaatc atcgataaca gccgttcctg aataaggttt gttggcttca cgaataacct
     7201 tgcgtcctga gaattcttcg atttttgtca ggatttcttc tgggaatccg ttccagaaag
     7261 tatcgaaagg ctcagtaatg ttgagtccca tgatttccca gtgtccagtc atagtatcct
     7321 taccaagaga tacttcctct aattttgttg catatccagt tggattgatt tcagctgcta
     7381 cagtcttaag aggagtttca cgaggaatat ttccaagacc tattttagcc atgtttggga
     7441 cattcaaacc aactgttttt gaaatgtgtc ccagtgtgtc agaagctcca tctggaaccc
     7501 ctgcattgac aaagttatta gcatctggtg ctgcaccgat tcctacagaa tccagtacca
     7561 ccaaatgaat acgattaaat ttagacatct tatgccccct tctattttcc tttttttgaa
     7621 gttgaaatct gaactccatc tcgtccagca acgattacct tatccaccat ttggttgaat
     7681 aaaccatgct ccacaacacc aacgacatgg tccaattctt gtccaaaagc aattggattt
     7741 tcaatgacat ccaaggcgag gtcaatgata aaattctgca tatccgtcac aaaacgttgg
     7801 ccgtcttttt cacggaaact tggtttgtag ccagctcgtt caaaatgacg aaagacctgc
     7861 tctgcaccat actgaaccac ttctactggc aatttaaaag cacctagttt ttcgaccagc
     7921 ttgctttcat ccaccaccca aatgtattct tttgatggtg ttgcgaccac cttttccatg
     7981 agaagggcac caccaccgcc tttgattcca ttaaactgac tatccacttc atccgcgcca
     8041 tcaaccgtca catcgacaaa gtctacttgg tcaatagact tgagcgggat attaaggcct
     8101 tctgcctgtt tagtggtcac actagaagtt gttacagctg taatctgcaa tccttcttcc
     8161 ttgatacgac gaccgatttc ttcgacaaaa taataggcag tcgagcctgt tccaagtcca
     8221 acgaccattc catcgctcac gaactcagca gccttgatac ctgccatttt cttcagattt
     8281 tccaccgatt tacctccatc attctcagtt cttattttac tatgaaaccg ctttttattc
     8341 aagtaaaaac atcaaatcta catttttcat caatctttct ttaaaaatat gcattttatt
     8401 tttaccaaac gaataaaaat gagtttatct tttatgtatt tgatttgttc atctctttcc
     8461 agttacaaaa acaaactctt aactttgata cgtctagttt agccttaaag caggcgcatt
     8521 tcaagaatca taaattaaga gaaggaagta gatttatctg tctattttaa acaagtttat
     8581 ctttgcttac cctctcttgt tgacaaataa ttttcattat tttgggaaga tacctgctga
     8641 tttccgtcgg taagaccaca taattttcct gagatagttc ttgggctatg gctgaatgaa
     8701 gatgggtcgc tactgccaca cgctcgtaga gactggcctg tcggaattgg cctacaaatc
     8761 ctgcgatcat cccagccaga gtatctccca ttcccccagt cgcctgataa ggaccaccaa
     8821 cctgtaactg gtaataatca gattggccaa cttcccaaat acgagtagct gggcctttct
     8881 ccaccaaaat tgttccttga gggaaggaag tcagaacact agccgttgca tcttcctttt
     8941 gcttttcaat cgtaattcca gataattttt cccattcttt ttggtgagga gttaggataa
     9001 gctggcttga gggaaatgac aaacttgtcc tagcaagaat ggtcaaggca cctccgtcta
     9061 caatcaaaat ctgattttgg cttaaattaa caaagacctg ttttactaga ttttctccag
     9121 aagcatcgtc tcgtaaacca ggccccaaca agacaacttc tgccttctcc aattgctctt
     9181 ttaacaattg ctggtcttga agagaaaagg ccataggctc aggtaaatgg ctgtgcagag
     9241 ccgggatatt ttccctgtcc gttccaacgg tcaccaatcc tgcaccgctt tttacagctg
     9301 ctaaagcagc catgatgatg gcacctccat aaggataagt accaccaagc agcagcagac
     9361 gaccataatc tcctttatga cttgaacgag aacgttcaat aataactttt tctaataagg
     9421 tttgattaat cactttcatc cttttttcct ctcactttta ttatacaata aaaaggagac
     9481 gcagacctcc ttttgtaatc ttatatctaa aatttaatat tcatttctgc cattttagat
     9541 atagctatag aaaatacact ctattaatcg aatgtttctc ttattttcta tccaatgtcc
     9601 gaagtgctgc ttgataagtt tgctccatca gcatggtaat ggtcatagga ccgacacctc
     9661 cagggactgg cgtgatatag ctagcaagtg gtgcaactgc ctcataatca acatctccac
     9721 agagcttccc attttcatct cggttcatcc caacgtcaat gacaaccgca cctggtttga
     9781 caaagtcagc agtcacaaac ttggcgcggc cgattgcgac tacaagaata tctgctttag
     9841 cagccacctt ggcaagatta tgagttcgtg agtgggtcaa ggttactgtc gcatttttag
     9901 ccaaaagaag ctgagccata ggttttccaa cgatatttga acgaccgatg acgaccgcat
     9961 ttttaccttc caagtcaatc ccatattcat gaaacatttc cataattcct gcaggtgtcg
    10021 agggaatcat gactggatgt ccagaccaaa gacgtcccat gtttaaggga tggaaaccat
    10081 ccacatcctt ttctgggtca atggctaata aaaccgcctc ttcatcgata tgttttggta
    10141 atggcaactg gaccaaaatc ccatgccaag ctggatcctg attatattta gcaatcaggt
    10201 ctaacaattc ctcttgagta atggtctctg gaactcgcac tacttcgcta cgggaaccag
    10261 ccgcaagagc tgacctctcc ttgttgcgaa cgtagacttg gctggctgga ttatccccaa
    10321 ccaaaatcac taccaaacca ggcactagag ctgtttcttc ctttaatttt gcagtctttt
    10381 cagccaactg cccctgcaat ttggccgcta aagctttccc atcaataatc tgtgtcatat
    10441 cacttctctt ttctttcaaa tatcttccat tataccaaaa actgctagtg ccctctaaca
    10501 cttctttcct aggttatcct atagtttcaa actataagaa aaagctctgg ttgaactttt
    10561 cactaatctt gtcttgaaat tttaaaatag attataaaac cttactcaag aaatctttag
    10621 tccgttgttc ttgggtttgt tcaaaaatct gctcaggtgt cccatcttca acaaccacac
    10681 cgtctgccat aaagataaca cgatctgcca cctcacgggc aaatcccatc tcatgtgtta
    10741 cgataaccat agtcatccct gacttggcaa ggtcttgcat aacagccaat acctcaccta
    10801 ccatttcagg atccaaggct gaagttggtt cgtcaaagag caaaacatct ggttccatag
    10861 ccaacccacg cgcgatggca atccgttgtt gttgcccacc cgacagactc tgtggataag
    10921 cgtctgcctt atctggcaaa ccaacttttt ccaaaagctc ttgggctctc ttctcagcaa
    10981 ctttcctgct ttcaccttta gtcttgatag gtgacaaggt aatattttcc atcacagtca
    11041 tattaggaaa gaggttaaat tgttggaaaa ccatgcccat cttctcacgc atggcaaaca
    11101 ggtcattctt cttgtccgta atatcgactc cctcaaagat aaccttcccc ttggttgctt
    11161 cctccaacaa attcatagag cgaagcaagg tagatttccc actccctgaa ggaccgatga
    11221 taacgacaac ttctcctctt ttaatctcga ggttgatgcc cttcaatact tcattctttc
    11281 caaaagattt atgtaaattt tcaattttta tcaaggtttc tgtcattatt tcttatctcc
    11341 ttgtcccata tgtttttcaa aagctttcaa ggctactgtc agaatagagg tcataatcaa
    11401 gtagtaaaat gctgcaaata aaagtggtgt taaaggtaga taggttgttg tagaaactgt
    11461 tgtagcccca ttccacaact ccatgacccc aatagctgat aagagggagc tgtccttgat
    11521 aatggtgata aattcgttcc ccaatgctgg caagatattt ttgactgctt gtggcaaaat
    11581 cacataacgc atcgcatttt taggacgaat ccctagcgaa taagccgcct ctagctgacc
    11641 ttttggaacc gcattgattc cggcacgaac agtctccgaa acataagcac cactattcat
    11701 agagataatc aaaatccctg gaatcagacg cgaaaaatca acacctaaaa ttccaatctg
    11761 aatagtcgga gcattgatat gcataagagc aaaggcaatc ataatttgaa ccatcatcgg
    11821 tgtcccacgg aaaatccaaa cgtacaagtt ggccaaccaa acaagcggtt taaactttga
    11881 acgttgccca aaagccaaga caacacccaa aatagttccc aaaaagataa cacagataga
    11941 aataagaatc gtcacaacag ccccatagtt aaaataaggt aaatacttag gtaaaaaaga
    12001 aaaattcata gtcacactgc tttctaaaat agaatactgt atgattataa catgttttga
    12061 attaaaattt aaatttttta tccaatttat ttaaaaatct ttaaactagc agaattaacg
    12121 agaaatctta gttttttcta gtcaagttgg cctcctttat ggtaaaatag taacgataag
    12181 aaatgaagga gaatcaaaat ggaatcacat ttggttagaa tcatcaaccg ccttgaagca
    12241 atggcaaacg atggcggaaa tttaaaacgt aattttgaac gcgaaggagt tgttgttgca
    12301 gaagttgcgt acagccacga cgaagaaaac ggcccaatct ttacccttcg tgacgtagaa
    12361 gatcgcgaaa cgtatacgtt tgatagcatt gacttaatcg caatggaaat ttacgaactt
    12421 ctctactaat ctaaaacaag ctgggattgt ccctgcatgt ctaagtaaaa tcctttttgt
    12481 ctcacaaata ggattttttt atagtccaaa ttcacaaaaa ttttcattgt aacaacttct
    12541 caaagcttca atctttttac ttgctttaca ccccaatcct gttataatga gagtatagaa
    12601 tttgactatt tttgaccttt aaacaagtca ggctaaagaa agaaatgagg tagatgtatg
    12661 ctttgtcaaa actgtaaaat taacgactca acaattcatc tttacaccaa tctcaatgga
    12721 aaacaaaaac aaattgacct ctgtcaaaac tgctataaga ttatcaaaac agatcctaac
    12781 aatagcctct tcaaaggtat gacggatctg aacaatcgtg acttcgatcc ctttggtgat
    12841 ttcttcaatg atctaaacaa tttcagacct tctagcaata ctcctcctat tcccccaacc
    12901 caatcaggtg gaggttacgg tggaaacggc ggttatggtt cccaaaatcg tggatctgct
    12961 caaactccgc cacctagcca agaaaaaggc ctgctggaag aatttggtat taatgtaact
    13021 gaaattgccc gtcgtggaga cattgacccc gttattgggc gcgacgatga gattatccgt
    13081 gtcatcgaga ttctcaatcg tagaaccaag aataatcctg tccttatcgg tgaacctggt
    13141 gtcggaaaaa cggccgttgt cgaaggtcta gctcagaaaa ttgtcgatgg cgatgtgcca
    13201 cataaactcc aaggtaaaca agtcatccgt ctggatgtgg ttagcttagt tcaaggaacg
    13261 gggattcgag gacaatttga agaacgcatg caaaaactca tggaagaaat tcgcaaacgt
    13321 gaagacatca tcctctttat cgatgaaatc catgaaattg ttggtgctgg ttctgcgagt
    13381 gatggtaata tggacgcagg aaatatcctc aagccagccc ttgctcgtgg agaactgcaa
    13441 ctagtcggtg ctactaccct caatgaatac cgtatcattg aaaaggatgc tgccctcgag
    13501 cgtcgtatgc agcctgttaa agtcgatgaa ccaacggtgg atgaaacaat cactattctc
    13561 aaagggattc aaaagaaata cgaagattac caccacgttc aatataccga tgctgcgatt
    13621 gaagcagctg caactctttc caatcgctac atccaagatc gcttcttgcc tgacaaggcc
    13681 attgacctcc tagatgaagc tggttctaag atgaacttga ccttgaattt tgtggatcct
    13741 aaagtaattg atcagcgctt gattgaggct gaaaatctca agtctcaagc tacacgagaa
    13801 gaagattttg agaaggcggc ctacttccgc gaccagattg ccaagtataa ggaaatgcaa
    13861 aagaaaaaga tcacagacca ggatactcct atcatcagcg agaaaactat tgagcacatt
    13921 atcgagcaga aaaccaatat ccctgttggt gatttgaaag agaaagaaca atctcaactc
    13981 atccatctag ccgaagatct caagtctcat gttattggcc aagatgatgc agtcgataag
    14041 attgccaagg ctattcgccg taatcgtgtc ggacttggta cccctaaccg cccaatcgga
    14101 agcttcctct tcgttgggcc aactggtgtc ggcaagacag aactttccaa acaactggct
    14161 atcgaacttt ttggttctgc tgatagtatg attcgctttg atatgagtga atacatggaa
    14221 aaacatagtg tggctaagtt ggtcggcgct cctccaggtt atgttggcta tgatgaggct
    14281 ggtcaattaa ctgaaaaagt tcgccacaat ccatattctc tcatccttct cgatgaagtg
    14341 gaaaaagctc acccagatgt tatgcacatg tttcttcaag tcttggacga tggtcgtttg
    14401 acagacgggc aaggacgcac cgttagcttc aaggatgcta tcattatcat gacctcaaat
    14461 gcaggtacag gaaagaccga agctagcgtt ggatttggtg ctgctagaga aggacgtacc
    14521 aattctgtcc tcggtgaact cggtaacttc tttagcccag agtttatgaa ccgttttgat
    14581 ggcattatcg aatttaaggc tctcagcaag gataacctcc ttcagattgt cgagctcatg
    14641 ctagcagatg ttaacaagcg cctctctagc aacaacattc gtttggatgt aactgataag
    14701 gtcaaggaaa agttggttga cctaggttat gatccaaaaa tgggagcacg cccacttcgt
    14761 cggactattc aagactatat tgaggacaca atcactgact actaccttga aaatccaagc
    14821 gaaaaagatc tcaaagcagt tatgactagc aagggaaaca ttcagattaa atctgccaaa
    14881 aaaactgaag ttaaaagttc tgaaaaagaa aaataaatcc tataaaaaaa gagtagaaaa
    14941 tgaaattttt ctgcttcttt ttttactaaa ataactgtaa tttcttgaca gcttgccctt
    15001 tgtccattat ga
//
LOCUS       DAVKMU010000047        13559 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKMU010000047 DAVKMU010000000
VERSION     DAVKMU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.047"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(157..234)
                     /locus_tag="VW343_001684"
                     /pseudo
     CDS             complement(157..234)
                     /locus_tag="VW343_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            305..961
                     /locus_tag="VW343_001685"
     CDS             305..961
                     /locus_tag="VW343_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9756206.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTLVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            951..1274
                     /locus_tag="VW343_001686"
     CDS             951..1274
                     /locus_tag="VW343_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9756207.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKHKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1377..2207
                     /locus_tag="VW343_001687"
     CDS             1377..2207
                     /locus_tag="VW343_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9756208.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2361..3215
                     /locus_tag="VW343_001688"
     CDS             2361..3215
                     /locus_tag="VW343_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9756209.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3315..4688
                     /locus_tag="VW343_001689"
     CDS             3315..4688
                     /locus_tag="VW343_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9756210.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4681..5742
                     /locus_tag="VW343_001690"
     CDS             4681..5742
                     /locus_tag="VW343_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9756211.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            5744..6436
                     /locus_tag="VW343_001691"
     CDS             5744..6436
                     /locus_tag="VW343_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756212.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6465..7034)
                     /locus_tag="VW343_001692"
     CDS             complement(6465..7034)
                     /locus_tag="VW343_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9756213.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7169..7783
                     /locus_tag="VW343_001693"
     CDS             7169..7783
                     /locus_tag="VW343_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9756214.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7785..9431
                     /locus_tag="VW343_001694"
     CDS             7785..9431
                     /locus_tag="VW343_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9756215.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9443..10729
                     /locus_tag="VW343_001695"
     CDS             9443..10729
                     /locus_tag="VW343_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9756216.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10781..11314
                     /locus_tag="VW343_001696"
     CDS             10781..11314
                     /locus_tag="VW343_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756217.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11392..11862
                     /gene="nrdI"
                     /locus_tag="VW343_001697"
     CDS             11392..11862
                     /gene="nrdI"
                     /locus_tag="VW343_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9756218.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12155..13417)
                     /locus_tag="VW343_001698"
     CDS             complement(12155..13417)
                     /locus_tag="VW343_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9756219.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIIIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3953 a         2544 c         3110 g         3952 t
ORIGIN      
        1 gctatataat agtccatgct gtggctggat tcttttcagc ctacttgtac tcttcgaaac
       61 tctcttcaaa ccacgtcagc ttcactttgc cgtagtacgg ttactgactt cgtcagttct
      121 atccacaacc tcaaaacagt gttttgagct gacttcgtca gttctatcca caacctcaaa
      181 acggtgtttt gagcaacctg cggctagttt cctagtttgc tctttgattt tcattgagta
      241 ttattaagac aatatgagga gaaagatgaa agaaaaagga ttttaggagg gggcgcaggc
      301 agccatgcca acggctcttg gctatgtcag tatcggcctg gcctgtggaa ttatcggtgc
      361 gccctatgtg acacttgttg agatgggctt gatgagtctc tttgtttatg ctgggagtgc
      421 ccagtttgcc atgttggcac tgattgtggt tcaagctcct gtggcagcta ttgctatgac
      481 ggtttttcta atcaacttgc gtctcttttt gttgagttta cacgcatcga cttatttccg
      541 tcataccagt ctttggtaca atatcggtat gtctagtatc ttgacagatg agacctatgg
      601 cgttttgatg ggtgaattgg cccatacaga caaggtaaat cctatgtgga tgcacgggaa
      661 caatctcaac agctatgtgg cttggtttgt ggggacagta gtcggaacgg ctctgggtgg
      721 cctgctacca aatccagaaa tctttggctt ggattttgcc ctggttggga tgtttattgg
      781 tatttttgct tcgcaatttc agattatgca aagacggatt cctgtccgca atctgctcat
      841 tatcctagca gttgttgcgg tgtccttctt tttgctcttg acagtggtgt ctcagtcact
      901 agctgttctg tttgcgacgc tacttggttg tagcatgggg gtggttttag atggtcagta
      961 agtatctttt attagcagtt attttctctg gtcttgtgac ctggattccc cgtatgattc
     1021 ccttcatctt ggtcaagcat aagggcctgc ctgcgattgt tgagcgtttt ttgaagtttt
     1081 tgcctgtttc cattatcttt gccttgattc tttcaagcgt agtgacaggt aaggttggga
     1141 gccttcctca aattaaatgg ctagacttct tagccgtctt tccaacagct tgggtagcct
     1201 ttcgctaccg caatctagtc ggaacagttc tctttggagt ggtcttgatt gccatcttgc
     1261 gtttggtctt ttaaattacc cataaaaaaa cttatcacag agatagatat catataatgg
     1321 cgtaaatgct ctttttctgt taagattata aggtattcta ttttggagga aatgacatga
     1381 aaaaaatcgt taaatactca tctcttgcag cccttgctct tgttgctgca ggtgtgcttg
     1441 cggcttgctc aggtggtgct aagaaagaag gacaagcagc tagcaagaaa gaaatcatcg
     1501 ttgcaaccaa tggatcacca aagccattta tctatgaaga aaatggcgaa ttgactggtt
     1561 atgagattga agtcgttcgc gctatcttta aagattctga caaatataat gtcaagtttg
     1621 aaaagacaga atggtcaggt gtctttgctg gtcttgatgc tgaccgttac aatatggctg
     1681 tcaacaatct tagctacact aaagaacgtg cggagaaata cctctatgcc gcaccaattg
     1741 cccaaaatcc taatgtcctt gtagtgaaga aagatgactc tagtatcaag tctctcgatg
     1801 atatcggtgg aaaatcgacg gaagtcgttc aagccactac atcagctaag cagttagaag
     1861 catacaacgc tgaacacacg gacaacccaa ctatccttaa ctatactaag gcagatttcc
     1921 aacaaatcat ggtacgtttg agcgatggac aatttgacta caagattttt gataaaatcg
     1981 gtgttgaaac agtgatcaag aaccaaggtt tggacaactt gaaagttatc gaacttccaa
     2041 gcgaccaaca accgtacgtt tacccacttc ttgctcaggg tcaagatgag ttgaaatcat
     2101 ttgtagacaa acgcattaaa gaactctaca aagacggaac gcttgaaaaa ttgtctaaac
     2161 aattcttcgg agacacttat ctaccggcag aagctgatat taaataattt cttgaaatca
     2221 tccattctga gtaagatggg tggtttctca gtttttaggg atatagtttt aaactatata
     2281 tctgactatc taataacaat ttgtgaaatc aaacaaattg tgagatacta gtacggtatt
     2341 atttttaagg agaaagaatc atgaaaatca aaaaatggct tggcctagca gcccttgcta
     2401 cagtcgcagg tttggctctt gcagcttgcg gaaactcaga aaagaaagca gacaatgcaa
     2461 caactatcaa aatcgcaact gttaaccgta gcggttctga agaaaaacgt tgggacaaaa
     2521 tccaagaatt ggttaaaaaa gacggaatta ccttggaatt tacagagttc acagactact
     2581 cacaaccaaa caaagcaact gctgatggcg aagtagattt gaacgctttc caacactata
     2641 acttcttgaa caactggaac aaagaaaacg gaaaagacct tgtagcgatt gcagatactt
     2701 acatctctcc aatccgcctt tactcaggtt tgaatggaag tgccaacaag tacactaaag
     2761 tagaagacat cccagcaaac ggagaaatcg ctgtaccgaa tgacgctaca aacgaaagcc
     2821 gtgcgcttta cttgcttcaa tcagctggct tgattaaatt ggatgtttct ggaactgctc
     2881 ttgcaacagt tgccaacatc aaagaaaatc caaagaactt gaaaatcact gaattggacg
     2941 ctagccaaac agctcgttca ttgtcatcag ttgacgctgc cgttgtaaac aataccttcg
     3001 ttacagaagc aaaattggac tacaagaaag cacttttcaa agaacaagct gatgaaaact
     3061 caaaacaatg gtacaacatc attgttgcga aaaaagattg ggaaacatca cctaaggctg
     3121 atgctatcaa gaaagtaatc gcagcttacc acacagatga cgtgaaaaaa gttatcgaag
     3181 aatcatcaga tggtttggat caaccagttt ggtaataaga aacagggagg tgggagagaa
     3241 aattccacct cttgcttttg tatagagtat agattgtaaa gaagactatt cgttcataga
     3301 aaggtagaga gaatatggtt tttcctagcg aacaagaaca gattgaaaaa tttgaaaagg
     3361 atcatgtagt ccaacattat tttgaggttt tgcgtacctt gatttctaag aaatcagtct
     3421 ttgcccagca ggttggactc aaggaagtcg caaattatct gggtgagatt ttcaagcgtg
     3481 ttggagctga agtggagatt gatgagagct atacagcgcc ctttgtcatg gcacatttca
     3541 agagttcgcg tccagatgcc aagaccttga ttttctataa ccactatgac actgtgccag
     3601 cggatgggga tcaggtctgg acagaggatc cttttacgct ttcggtccgc aatggcttca
     3661 tgtatgggcg tggggttgat gacgacaagg gtcatatcac agctcgcttg agtgctttga
     3721 gaaaatatat gcagcaccat gatgatttac ctgtcaatat cagctttatc atggagggag
     3781 cggaggaatc ggcttcaaca gacctagata agtatttgga aaagcatgca gacaaactcc
     3841 gtggggcgga tttgttggtc tgggaacaag ggaccaaaaa tgccttggaa cagctggaaa
     3901 tttctggtgg caataagggg attgtgacct ttgatgccaa ggtaaaaagc gctgatgtgg
     3961 atatccactc gagttatggt ggtgttgtgg aatcagctcc ttggtatctc ctccaagcct
     4021 tacagtctct tcgtgctgcg gatggccgta tcttggttga aggcttgtac gaagaagtac
     4081 aagagcccaa tgaacgagaa atggccttgc tagaaactta tggtcaacga aacccagagg
     4141 aagttagtcg gatttatgga ttggagttgc ctctcttaca ggaggagcgg atggcctttc
     4201 taaaacgttt ctttttcgag ccagcgctta atatcgaagg aatccagtct ggttatcaag
     4261 gtcagggtgt taagactatt ttacctgcag aagccagtgc caagctagag gttcgtctgg
     4321 ttccgggcct agaaccgcat gatgttctgg aaaaaattcg gaaacagcta gacaaaaatg
     4381 gctttgataa ggtagaatta tactatacct tgggagagat gagctatcga agcgatatga
     4441 gcgcaccagc cattctcaat gtgatcgagt tggccaagaa attctatcca cagggcgttt
     4501 cagtcttgcc gacgacagcg gggacaggac ctatgcatac ggtctttgat gccctagagg
     4561 taccaatggt tgcattcggt ctaggaaatg ccaatagccg agaccacggt ggagatgaaa
     4621 atgtgcgaat cgctgattat tacacccata tcgaattagt agaggagctg attagaagct
     4681 atgagtagag atattatcaa gttagatcag atcgatgtga cttttcacca aaagaagaga
     4741 accattacag cggttaagga tgtgaccatt cacatccaag aaggggatat ctacggaatc
     4801 gttggatatt ctggagcagg aaaatcaacc cttgtacggg tgattaatct cttgcaaaaa
     4861 ccatctgcag ggaaaattac cattgacgac gatgtgattt ttgacggcaa ggtgaccttg
     4921 acggcagagc agttgcgtcg taaacgtcaa gatatcggaa tgattttcca gcattttaac
     4981 ctgatgagcc aaaagacagc agaggagaat gtagcctttg cccttaaaca ctctgaactc
     5041 agcaaggaag aaaagaaggc taaagtagct aagttgttgg acttggttgg tttggcagat
     5101 cgtgctgaaa actacccttc acaactatct ggagggcaaa aacagcgtgt ggcaattgcg
     5161 cgtgccttgg ccaatgatcc aaaaatcttg atttcagacg agtcaacttc tgcccttgat
     5221 ccgaagacaa ccaagcagat tttggccttg ttgcaagatt tgaaccaaaa attaggcttg
     5281 actgttgtct tgattacgca tgaaatgcag attgtcaaag acattgccaa ccgtgtggcg
     5341 gttatgcagg atgggcattt gattgaagag ggtagtgtgc ttgaaatctt ctcagaccct
     5401 aaacaacctt tgactcaaga ctttatctca acagccacag gtattgacga agccatggtc
     5461 aaaatcgaga agcaagaaat cgtggaacac ttgtctgaaa acagtctctt ggtgcaactc
     5521 aagtacgctg gatcttcaac agacgagcca cttttgaatg aattgtacaa gcattatcaa
     5581 gtaatggcta atattctcta tgggaatatc gaaatcctcg atggtactcc tgttggagaa
     5641 ttggtggtgg tcttgtcagg tgaaaaagca gcgttggcag gtgcccaaga agccattcgt
     5701 caagcaggcg tacagttaaa agtattgaag ggaggacagt aagatggaat cattgattca
     5761 aacctatttg ccaaatgtct ataagatggg ttggtctggt caggcaggct ggggaacagc
     5821 tatctaccta accctctata tgacagttct ttccttcatt atcggaggct tcttggggct
     5881 agtggcaggt ctctttctcg tcttgacagc gccaggtggt gtcttggaga ataaagtcgt
     5941 attctggatt ttagacaaaa ttacctcaat ttttcgtgcg attcccttta tcatcctctt
     6001 ggcaatcttg tcaccacttt ctcacttgat tgttaaaaca agtatcgggc caaatgcagc
     6061 ccttgtccca ctttcttttg cagtctttgc cttctttgcc cgtcaggtgc aggttgtctt
     6121 ggctgaattg gatggcggtg tcattgaggc ggctcaagcg agcggagcga ctttctggga
     6181 catcgtgggt gtttacctat cagaaggtct tccagatttg atccgtgtga cgactgtgac
     6241 cttgatttcc cttgttgggg aaacagctat ggccggtgcg gttggagctg gtggtatcgg
     6301 taacgtagcc atcgcttatg gatttaaccg ctacaatcac gatgtgacca tcttggcaac
     6361 cattattatc attttgatta tctttgcaat ccaattcttg ggagatttct tgactaagaa
     6421 attgagtcat aaataaaaag agccgcgtgg ctctttttaa ctgatcagat tttctgtgca
     6481 aattttttac tcaaggcttg tccaatcaag gcacccacta gggctccgat gacaatactt
     6541 gcgataaata gaaggacagt tccagggttt ggagcgacca tgatacggtc gatatattct
     6601 tgggattttc ctcttgccag aagagtagcc atataggctt tgggcgcaat ccacataagc
     6661 aagattggtc ctgttgtact aaaggcgaaa ataatgaaag aaaggaagtt ctttgttttg
     6721 tccttgtatt ttcctaaatg agctacttca tctgctagga ggccacagat aattccagga
     6781 aggaaggaac cagcaccgtg tttagttccc aagaaaaaga gggcaatgac aaggccgata
     6841 gtggtaatgg ctccaaagcg cggaactttt gcgactagga tcatatagac gctaccgccg
     6901 acaagggcag taaaggcagg cgcataaaac atgtttccag tttggtcaaa gagattgccc
     6961 aaaaggacac caatccccat gcagaggaag taaagaactg caaaaagcag tgtagttaag
     7021 atagatttct tcatgaattg tctcctgata attttttcac aattctcatt gtaccacggt
     7081 ttggtgggat tgtaaatgga tactagaagt ttttgaaaac gcttgaaata tgatataatg
     7141 gtttcatagt tatttttagg ggaatatcat gggtagattt ttagactttg tctttaatcg
     7201 tttcttttta gggatgattg cgacagcctt cttttggcta ttaactttag caggagggat
     7261 tatccttggt ctagcgccgg ctagtgccac cttgatgagc ttatatgcag aacatggtta
     7321 tagctttcgg gaatacagtt tgaaggaggc ttggtctctt tacaagcaaa attttgtctc
     7381 aagcaacctg attttctata gctttttagg tgtgggtcta gttttgacct atggtttgta
     7441 tctcttggtg caattgcctc atcagaccat tgttcatttg attgcgaccc ttttgaatgt
     7501 cctagtagtt gccctgatct ttttggctta tacagtatct ttaaaattac aagtttattt
     7561 tgccttgtcc tatcgaaata gtctcaaatt atccttgatt ggcatcttta tgagtctagc
     7621 agctgtggcc aagattctcc ttgggactgt gctacttgta gcaattggtt attatatgcc
     7681 tgccctgcta ttttttgtag gaattgggat gtggcatttc tttatcagtg atatgttgga
     7741 acctgtctat gaaatcatcc atgaaaaatt ggcgacaaaa tagaatgaag cagttttggc
     7801 tacatacgct tctaagaacc tatagttcag tgatgatcat tatcattgcg agttttgcaa
     7861 tcttactctc ttacgctgac tgggattcac gtgaaaagga agcccagaga gtagcccagc
     7921 gtgtaactgc tagaacagtg agtgaaattg aatattacca tagagagtca acccagatag
     7981 ctcaggcttt agttgaaaac caagctcgta ttgagggaat ctataaatac tttagtctta
     8041 gcatgccaga ctatttttac tggcaattag agcggaaagc ttcgccttat atatcagtct
     8101 ctctgtatga aaatgttgat gacctctatg ttcgaaatga ttttgtaact ggagtggcca
     8161 ttgcttttca agattacaag gaagtctatg tttctactaa agacaaacgt agtggagaaa
     8221 aaatcagggc tgaggatttc aaaccagcag gaaatagttt tgccattccg gtgtcagatc
     8281 cagtgtcaga tcaagattta ggagtgattt acatctcctt ggatcctgct gttttatacc
     8341 atgccattga taatactaga ggtcatactc cgatggcagt aacagtgacc tcaccttttg
     8401 atacggagat ttttcatatt ggtgagacag ttgataagga gagtgaaaat tggctagttg
     8461 gcttaacttc tcatggatat caggttcagg tggcagttcc taaaaacttt gttttacaag
     8521 gaacagtgac tagctctgct ttgattgtgg gtttgagcct tctctttatt gtcattcttt
     8581 atctgacttt gaggcagact tttgctaatt accaaaagca ggtagtggat ttagtagaat
     8641 ccattcaagt cattgctcaa ggcgaagagg ggcgtcggat tgacatttcc gagaaagatc
     8701 aggaattact cctaatcgcg gagacgacca atgatatgtt ggatcgattg gaaaagaata
     8761 tccatgatat ttaccagtta gagcttagtc aaaaagatgc caatatgcga gccttgcagg
     8821 cgcaaatcaa tcctcatttt atgtataata cgctggagtt cttgcgcatg tatgcagtta
     8881 tgcagagtca agatgagttg gcagatatca tttatgaatt cagtagtctc ttgcgtaaca
     8941 atatttccga cgaaagagag accctcctca aacaggaatt agaattttgc cgtaaataca
     9001 gctatctctg catggttcgc tatcccaagt ccattgccta tggtttcaag atagatccag
     9061 agttagagaa tatgaagatt cccaagttta ccttgcaacc gctggtagaa aactatttcg
     9121 cgcatggtgt tgaccacagg cggacagata atgtgattag catcaaggct cttaaacagg
     9181 atggttttgt ggaaattttg gtggtcgata atggtagagg aatgtcggct gaaaagttgg
     9241 caaatatccg agaaaaatta agtcagagat attttgaaca ccaagccagc tacagtgatc
     9301 aaaggcagtc tatcgggatt gtcaatgtac acgagcgttt tgtgctctat tttggagacc
     9361 gctatgccat tactatagag tctgcagagc aagccggtgt tcagtatcgt attacaattc
     9421 aagatgagta gaaagggaga aaatgtataa agtattatta gtagatgatg agtacatggt
     9481 gacagaaggt ctgaagcgtt tgattccctt tgataagtgg gatatggagg ttgtcgcaac
     9541 agccagtcat gccgatgaag ctctagaata tgttcaggaa aatcctgtcg atgtcatcat
     9601 ttccgatgtc aatatgccag acaaaacagg gcttgatatg attcgggaga tgaaagagat
     9661 cttaccagat gctgcctata tcctgctctc aggttatcag gagtttgatt atgtaaaaag
     9721 agcaatgaac cttagtgtgg tggactattt ggtcaagcct gttgataagg tagagctggg
     9781 aaatctgctg gagaagattg caggtcagct cggcgagaga gggaagaaaa gtcagactct
     9841 tagtcaagaa ttagacgagg ctggatttgt tagttattta ggggataagg agaattggtg
     9901 gataggtcta tccaaggaaa aacaaggttc cttcaccatt ccctactatg tcttgggtca
     9961 agactggcag attttcattt ctggccaacc cttagatggc ttagtcgtta ctccctttga
    10021 agctccctat caagaacact ttgaacgctg gaagctgaat gctgagaaaa ccctctttta
    10081 cggttctgta aatctgcagc agtctgagag tctctttgcc tattacgaac cgatttatag
    10141 ggttatcatt cagggaaatc tcaatcaaat cgtagaagag ttaaatctct tggagaaggt
    10201 agttcttgaa aatacgccgc gtgttccgat tactaaacag ctttttattc agtttgtcat
    10261 ggatgttttc catttatttg aacatctcaa agctgatgac ctgacagaca tcgttaaaac
    10321 cattcatgct attcaatcct tcgatgaatt ggttccttat atcaaggaaa ctctgaccag
    10381 ctttttcggt caataccgta tgaatgaaaa tgtggtcagt gtgctggaag tcattggtcg
    10441 tgattaccaa aaagagcttt ccctcaagga tatcagtaag gctctcttta tcaatcctgt
    10501 ctatctaggg cagttgatta agcgtgaaac cgattcgact tttgcagagt tactaaacaa
    10561 acaacgtatt aaggctgccc agcagctctt gctttcaact agtgacagca tcgaagatat
    10621 ttgttatgct gttggttaca gtaaccttgg atatttctat aaagttttcc gaaaattgtg
    10681 cggaaaatcg ccaaaagcct accgaaaaca ggtagaaact atactataag atttgtattc
    10741 ctttacaaaa tgtgctataa tattatcagg aggttctatc atgaaaaaga aaccaattta
    10801 tctatgggtc ttgctaatct tgtctgccct tatttcagtt ccgtctctgt ttggaattgt
    10861 gagtcccttg cctagtaaag aagcccttcg tgctgctcag aaacaagttg caggggtcaa
    10921 tgctcagcaa ttagaagacc agcttaatta tacctataga gtagcagaag catctcattc
    10981 tatttttaat gttgctttga ttgtgctatc tactatttta gtggtcgtag cgattgtctt
    11041 ccttgttcgt aaaaatttgc aatatgcaaa ctatacttac gttggctatg ttttgttagc
    11101 tattattggt tcgatttatg gctatgttgg tttacaagat gctgtgcagt tggttcaaga
    11161 tgagagcatg cgtttgacag tgagtattgg ttcaaaagct gttagcattt tctatatcgt
    11221 catcaatgtt ctcttcctag cccttgtctt ctataagatg tggcgacaac agaaagcctt
    11281 ggcagaagaa gaggaaacag aagagcttgc ctaagtattg acaaaaaaga actatcaaga
    11341 tacaatcttg gtagttgttt tttgattata gataaaatca tggaggtaca tatgaagaca
    11401 atttctttag tttatattag tttgagtggc aacactgaaa gttttgtgac gcgcttgaaa
    11461 gactatctct tgtcccagta caaagggatt gaggttcaaa agattcatat caaggatttg
    11521 gtcaaggaag gcaaaaattt ctacgaaatg gaccatccct atgttgcttt tttgccgacc
    11581 tatctcgaag gtgggaatgg cgtggataac ggagatgttg agattttgac gacaccggtg
    11641 ggagatttta ttgcctatgg taacaatgct agtaagtgtt ttggtgtggt tgggtcagga
    11701 aatcgtaact ttaataacca atactgccta acagccaagc aatacagtca acgctttggc
    11761 tttcctgtat tggctgactt tgaaatgcga ggcatgctgg aagatatcaa acatgtcgca
    11821 gctattatcg cagatttgta tgagttggaa aaggagaatt aatactcaat gaaaatcaaa
    11881 gagcaaacta ggaagctagt cgcaggttgc tcaaagcaca gctttgaggt tgcagataga
    11941 gctgacgtgg tttgaagaga ttctcgaata gtataaagtt cttataagag gtggttgaag
    12001 ttgttcaact gttttttgag tataaacagt ctttaagaac gtaaaaaagc ataagttcag
    12061 gtcttcaaaa ccttgttctt atgcttttta tcatagaaat atttgctgaa ttttctcttg
    12121 aaatgaaacc ttatcttcat ctcgcgcttg tcccttaccc tgcttttccg attgcgagtg
    12181 cgtagataaa gaagatggcg aagccaaaga ggaaaatcaa tcccgcaatg gcaaggtgtt
    12241 tgagccaaga aggaagattt ttgttttcac gtgttaccaa ggcgtaattc aaaagagcaa
    12301 agaaaggtgt tgtcaggaaa gagccaatca tggcaaagcg gagcatggtt gaaacctgac
    12361 cagcgaagaa cttgataatg atgataccga tgatagcagt gatggtcatc cagatgttca
    12421 aagatttacg attatcctct ttttgactga ttagcagtcg gagagattcc tgattaacgc
    12481 gagaatagcc atcgataaca gttataactg ttccaaagat acagaggaag gcaataaagg
    12541 taatcaagta acgggaccat tcgccaagaa cagaggcata catgcccacg aattgagaga
    12601 tgtatttggc tgaagcagct tcaaccgcct gccctgtagg atactgaatc agtgctccca
    12661 gtgccacaaa gaagacggct aggatagctg ttccaatata accagtgtta aagtcaaaca
    12721 gagcgtcttc tgtgttaaag ttgacggtct ttctcttttc agctgaccaa agtgaattga
    12781 tggctgaaat ttcaatagga gccggcatcc atcctaggag ggagacgatg aagggcagag
    12841 ctgccatttg ccaaggtgtc ttctcgacaa aatcagaact gtattctgga tgcttgaccg
    12901 ccgcaatgat aactgcaaga acagtcgcaa tggttaaggc agacataatc catttgacca
    12961 tgccgtctaa aagtttgtag cctccaaaga gtagcatagc ccaaatgatt gcaacgagaa
    13021 tgagggacca ctgagtaatg ctaagtccaa tcattgggaa ggcactggcg atgatagctg
    13081 agcacagaat ggcaacacca gccgtgttga ccatagccga aaagacattg aggataaaga
    13141 aaatccagag atagagtttt cctttttcgg cataaccttc aaccaaagtc tttccagtat
    13201 cagctgtgta ttcagcacca aaacggaaaa atggatattt aaagacattg gctaagatga
    13261 ccaagagaag tagagaccaa ccgtaagaac cgccagcttg agttgaggat acaatgtggg
    13321 aacctccaac agcggcagtt gccattagga ttccaggtcc cattgctttt agcttgcttg
    13381 cccaggttga ttgatttaag gaaatagctt gcgacatgat aaatactcct ttttgaattt
    13441 tcagaataat ctgtatatgt aatctatatt ctacaaaaaa ttttggaaaa atgcaagagt
    13501 attttgaaaa aagatagaaa atttacaaag aaaatctgaa ataactgtta tgatataga
//
LOCUS       DAVKMU010000048        12547 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKMU010000048 DAVKMU010000000
VERSION     DAVKMU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.048"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..1077
                     /gene="dnaN"
                     /locus_tag="VW343_001699"
     CDS             <1..1077
                     /gene="dnaN"
                     /locus_tag="VW343_001699"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9756220.1"
                     /translation="AISSKNAIPILSTIKIDVTNEGITLIGSNGQISIENFISQKNED
                     AGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQ
                     YPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVAT
                     DSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENI
                     SFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKD
                     GVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAV
                     RPFTLVPADTDEDFMQLITPVRTN"
     gene            1142..1336
                     /locus_tag="VW343_001700"
     CDS             1142..1336
                     /locus_tag="VW343_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9756221.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1420..2535
                     /gene="ychF"
                     /locus_tag="VW343_001701"
     CDS             1420..2535
                     /gene="ychF"
                     /locus_tag="VW343_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9756222.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLIESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYESEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2606..3175
                     /locus_tag="VW343_001702"
     CDS             2606..3175
                     /locus_tag="VW343_001702"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9756223.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     KADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3176..6685
                     /gene="mfd"
                     /locus_tag="VW343_001703"
     CDS             3176..6685
                     /gene="mfd"
                     /locus_tag="VW343_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9756224.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKELMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6743..7009
                     /locus_tag="VW343_001704"
     CDS             6743..7009
                     /locus_tag="VW343_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9756225.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7002..7370
                     /locus_tag="VW343_001705"
     CDS             7002..7370
                     /locus_tag="VW343_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9756226.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGVVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7490..8758
                     /locus_tag="VW343_001706"
     CDS             7490..8758
                     /locus_tag="VW343_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9756227.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKISFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8755..10032
                     /gene="tilS"
                     /locus_tag="VW343_001707"
     CDS             8755..10032
                     /gene="tilS"
                     /locus_tag="VW343_001707"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9756228.1"
                     /translation="MREQDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNIDVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10036..10578
                     /gene="hpt"
                     /locus_tag="VW343_001708"
     CDS             10036..10578
                     /gene="hpt"
                     /locus_tag="VW343_001708"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9756229.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10594..>12547
                     /gene="ftsH"
                     /locus_tag="VW343_001709"
     CDS             10594..>12547
                     /gene="ftsH"
                     /locus_tag="VW343_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9756230.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4315 a         1893 c         2556 g         3783 t
ORIGIN      
        1 gctattagtt ctaaaaatgc cattcctatt ttatcaacaa taaaaattga cgtgaccaat
       61 gaaggtatta ctttaattgg ttcaaatggt caaatttcaa ttgaaaattt tatttctcaa
      121 aaaaatgaag atgctggttt gttaattact tctttaggtt cgatccttct tgaagcttct
      181 ttctttatca atgtagtatc tagtttacct gatgtaactc ttgattttaa agaaattgaa
      241 caaaatcaaa ttgttttaac cagtggcaaa tcagaaatta ccctaaaagg aaaagatagc
      301 gaacaatatc cacgaatcca agaaatttca gcaagcactc ctttaatact tgaaacaaaa
      361 ttactcaaga aaattattaa tgaaacagcc tttgctgcaa gtacacaaga gagtcgtccg
      421 attttaacag gtgtccactt cgtattgagt caacacaaag agttaaaaac agttgcaaca
      481 gactctcatc gcctaagcca gaaaaaattg actcttgaaa aaaatagtga tgattttgat
      541 gtcgtaattc ctagccgttc tctacgcgaa ttttcagcgg tatttacaga tgatatcgaa
      601 actgtagaga ttttctttgc caataaccaa atcctcttta gaagcgaaaa tattagcttc
      661 tatactcgtc tcctagaagg aaactatcct gatacagatc gcttgattcc aacagacttt
      721 aacactacta ttacttttaa tgtggtaaac ttacgccagt caatggagcg tgcccgtctt
      781 ttatcaagtg cgactcaaaa tggtactgtg aaacttgaaa ttaaggatgg ggttgttagc
      841 gcccatgttc actctccaga agttggtaaa gtaaacgaag aaatcgatac tgatcaggtt
      901 actggtgaag atttgaccat tagtttcaac ccaacttact tgattgattc tcttaaagct
      961 ttaaatagcg aaaaggtgac cattagcttt atctcagctg ttcgtccatt tactcttgtg
     1021 ccagcagata ctgacgaaga cttcatgcag ctcattacac cagttcgtac aaattaagtg
     1081 aaagaggttg agcctggctc gcctctttta tgatataatc gaaaaagaaa aggagagtag
     1141 tatgtatcaa gttggaaatt ttgttgagat gaaaaaatca cacgcttgta caatcaagtc
     1201 gactggtaaa aaggctaatc gttgggaaat tacacgtgta ggagcagata tcaaaataaa
     1261 atgcagtaat tgtgagcatg ttgtcatgat ggggcgatat gattttgagc gaaaaatgaa
     1321 taaaattatt gactgagaac ccttagttag agggttagca ctttatccct ttttgtgtta
     1381 taatattagg gattgaaatg aaaacggaga atgagaaata tggctttgac agcaggtatc
     1441 gttggtttgc caaacgttgg taaatcaaca ctatttaatg caattacaaa agcaggagca
     1501 gaggcagcaa actacccatt tgcgactatt gatccaaatg ttggaatggt ggaagttcca
     1561 gatgaacgcc tacaaaaact aactgaaatg ataactccta aaaagacagt tcccacaaca
     1621 tttgaattta cggatattgc agggattgta aaaggagctt caaaaggaga agggctaggg
     1681 aataaattct tggccaatat tcgtgaagta gatgcgattg ttcacgtagt tcgtgctttt
     1741 gatgatgaaa atgtgatgcg cgagcaagga cgtgaagacg cctttgtgga tccacttgca
     1801 gatattgaca ccattaacct agaattgatt cttgcagact tagaatcagt caataaacga
     1861 tatgcgcgtg tagaaaagat ggcacgtacg caaaaagata aagaatcagt agcagaattc
     1921 aatgttcttc aaaagattaa accagtccta gaagacggga aatcagctcg taccattgaa
     1981 tttacagatg aggaacaaaa ggttgtcaaa ggtcttttcc ttttgacgac taaaccagtt
     2041 ctttatgtag ctaatgtgga cgaggatgtg gtttcagaac ctgactctat cgactatgtc
     2101 aaacaaattc gtgaatttgc agcgacagaa aatgctgaag tagtcgttat ttctgcgcgt
     2161 gctgaggaag aaatttctga attggatgat gaagataaaa aagagtttct tgaagccatt
     2221 ggtttgatag aatcaggtgt agataagttg acgcgtgcag cttaccactt gcttggattg
     2281 ggaacttact tcacagctgg tgaaaaagaa gttcgcgctt ggactttcaa acgtggtatg
     2341 aaggctcctc aagcagctgg tattatccac tcagactttg aaaaaggctt tattcgtgca
     2401 gtaaccatgt catatgagga tctagtgaaa tacgaatctg aaaaggccgt aaaagaagct
     2461 ggacgcttgc gtgaagaagg aaaagaatat atcgttcaag atggcgatat catggaattc
     2521 cgctttaatg tctaaaaatt aataaatggt gtcaattagg ttggaaaaaa attccaaccc
     2581 ttttggcttt tgaaaggaaa aataaatgac caaattactt gtaggcttgg gaaatccagg
     2641 ggataaatat tttgaaacaa aacacaatgt tggttttatg ttgattgatc aactagcgaa
     2701 gaaacagaat gtcactttta cacacgataa gatatttaaa gctgacctag catccttttt
     2761 cctaaatgga gaaaaaattt atctggttaa accaacgacc tttatgaatg aaagtggaaa
     2821 agcagttcat gctttattaa cttactatgg tttggatatt gacgatttac ttatcattta
     2881 cgatgatctt gacatggaag ttgggaaaat tcgtttaaga gcaaaaggct cagcaggtgg
     2941 tcataatggt atcaagtcta ttattcaaca tataggaact caggtcttta accgtgttaa
     3001 gattggaatt ggaagaccta aaaatggtat gtcagttgtt catcatgttt tgagtaagtt
     3061 tgacagggat gattatatcg gtattttaca gtctgttgac aaagttgacg attctgtaaa
     3121 ctactattta caagagaaaa attttgagaa aacaatgcag aggtataacg gataaatggt
     3181 gaccttatta gatttattct cagaaaatga tcagattaaa aaatggcatc aaaatttaac
     3241 tgataagaaa agacaactaa tacttggttt atcaacatct actaaggctc ttgcaattgc
     3301 aagcagttta gaaaaagaag ataggattgt gttattgacg tcaacttatg gagaagcaga
     3361 aggacttgtt agtgatctta tatctatctt gggtgaggaa ctcgtctatc catttttggt
     3421 agatgatgct cctatggtgg agtttttgat gtcttcacag gaaaaaatta tttcacgggt
     3481 tgaagccttg cgttttttga ctgattcatc taagaaaggg attttagttt gtaatatcgc
     3541 agcaagtcga ttgattttac cgtctcccaa tgcattcaaa gatagtattg taaaaatctc
     3601 agttggtgaa gaatatgatc aacacgcgtt tatccatcag ttaaaggaaa atggctatcg
     3661 aaaagttact caagtacaaa ctcagggcga atttagtctt cgaggagata ttttagatat
     3721 ttttgaaata tcccagttag aaccttgtcg aattgagttt tttggtgatg aaattgatgg
     3781 tatcaggtca tttgaagtag aaacacaatt atcgaaagaa aataagacag aactcactat
     3841 ctttccagct agtgatatgc ttttgagaga aaaggattat caacgaggac agtcagcttt
     3901 agaaaaacaa atttcaaaaa ctttatcacc tattttgaaa tcatacctag aagaaattct
     3961 ttcaagtttt caccaaaaac aaagtcatgc agactctcgg aagtttttat ctttgtgcta
     4021 tgataagaca tggactgtct ttgattatat tgaaaaagat actccaatat tctttgatga
     4081 ttatcaaaaa ttgatgaatc agtatgaagt ctttgaaaga gacttagcgc agtactttac
     4141 agaagaatta cagaatagta aagcattttc tgatatgcag tatttttctg atattgaaca
     4201 aatctataaa aaacaaagtc cagtgacctt tttctctaat cttcaaaagg gtttaggaaa
     4261 tctcaaattt gacaaaattt atcaattcaa tcaatatcct atgcaggaat ttttcaatca
     4321 gttttctttt ctaaaagaag aaattgaacg atataaaaaa atggattaca ccattattct
     4381 gcagtctagc aattcaatgg gaagtaaaac attggaggat atgttagagg aatatcagat
     4441 taaattggat tctagagata agacaaatat ctgtaaagaa tctgtaaact taatagaggg
     4501 taatctcaga catggttttc attttgtaga tgaaaagatt ttattgataa ctgaacatga
     4561 gatttttcaa aagaaattaa agcgtcgttt tcgaagacaa catgtttcaa atgcagagag
     4621 attaaaagat tacaatgaac ttgaaaaagg ggactatgtt gtccatcata tccatgggat
     4681 tggtcaatat ctaggaattg aaaccattga aatcaaggga attcatcgcg attatgtcag
     4741 tgtccaatac caaaatggtg atcaaatttc tatccccgtg gaacagattc atctactgtc
     4801 caaatatatt tcaagtgatg gtaaagctcc aaaactcaat aaattaaatg acggtcattt
     4861 taaaaaggcc aagcaaaagg ttaagaacca ggtagaggat atagctgatg atttaatcaa
     4921 actctactct gaacgtagtc agttgaaggg ttttgctttc tcagctgatg atgatgatca
     4981 agatgccttt gatgatgctt tcccttatgt tgaaacggat gatcaacttc gtagtattga
     5041 ggaaatcaaa agggatatgc aggcttctca gccaatggat cgacttttag ttggggatgt
     5101 tggttttgga aagactgaag ttgctatgcg tgcagccttt aaagcagtca atgatcacaa
     5161 acaggttgtc attctagttc cgacgacggt tttagcgcaa cagcactata cgaattttaa
     5221 ggaacgattc caaaattttg cagttaatgt tgatgtgttg agtcgcttta gaagtaaaaa
     5281 agagcagact gcaacacttg aaaaattgaa aaacggtcaa gtcgatattt tgattggaac
     5341 acatcgtgtt ttgtcaaaag atgttgtgtt tgctgatttg ggcttgatga ttattgatga
     5401 ggaacagcga tttggtgtca agcataagga aactttgaaa gaactgaaga aacaagtgga
     5461 tgtcctaacc ttgaccgcta cgccaatccc tcgtaccctc catatgtcta tgctgggaat
     5521 cagagatttg tctgttattg aaactccgcc gactaatcgc tatcctgttc agacctatgt
     5581 tttggaaaag aatgatagtg tcattcgtga tgctgtcttg cgtgaaatgg agcgtggagg
     5641 tcaagtttac tatctttaca acaaagttga cacaattgtt cagaaggttt cagaattaca
     5701 ggagttgatt ccggaggctt cgattggata tgttcatggt cgaatgagtg aagtccagtt
     5761 ggaaaatact ctattagact ttattgaggg acaatacgat atcttggtga cgactactat
     5821 tattgagaca ggggtggaca ttccaaatgc taatacttta tttattgaaa atgcggacca
     5881 tatgggcttg tcaaccttat atcagttaag aggaagagtc ggtcgtagta atcgtattgc
     5941 ttatgcttat ctcatgtatc gtccagaaaa atcaatcagt gaagtctctg aaaagagatt
     6001 agaagcgatt aaaggattta cagaattggg ctctggcttt aagattgcaa tgcgagatct
     6061 ttcgattcgt ggagcaggaa atcttttagg aaaatcccag tctggtttca ttgattctgt
     6121 tggttttgaa ttgtattcgc agttattaga ggaagctatt gctaaacgaa acggtaatgc
     6181 taacgctaac acaagaacca aagggaatgc tgagttgatt ttgcaaattg atgcctatct
     6241 tcctgatact tatatttctg atcaacgaca taagattgaa atttacaaga aaattcgtca
     6301 aattgacaac cgtgtcaatt atgaagagtt acaagaggag ttgatagacc gttttggaga
     6361 atacccagat gtagtagcct atcttttaga gattggtttg gtcaaatcat acttggacaa
     6421 ggtctttgtt caacgtgtgg aaagaaaaga taataaaatt acaattcaat ttgaaaaagt
     6481 cactcaacga ctgtttttag ctcaagatta ttttaaagct ttatccgtaa cgaacttaaa
     6541 agcaggcatc gctgagaata aggaattaat ggagcttgta tttgatgttc aaaataagaa
     6601 agattatgaa attttagaag gtctgctgat ttttggagaa agtttattag agataaaaga
     6661 gtctaaggaa gaaaattcca tttgatattt ttcttctata aaatagataa aaatggtaca
     6721 ataataaatt gaggtaataa ggatgagatt agataaatat ttaaaagtat cgcgaattat
     6781 caagcgtcgt acagtcgcaa aggaagtagc agataaaggt agaatcaagg ttaatggaat
     6841 cttggccaaa agttcaacgg acttgaaagt taatgaccaa gttgaaattc gctttggcaa
     6901 taagttgctg cttgtaaaag tactagagat gaaagatagt acaaaaaaag aagatgcagc
     6961 aggaatgtat gaaattatca gtgaaacacg ggtagaagaa aatgtctaaa aatattgtac
     7021 aattgaataa ttcttttatt caaaatgaat accaacgtcg tcgctacctg atgaaagaac
     7081 gacaaaaacg gaatcgtttt atgggagtgg tattgatttt gattatgcta ttatttatct
     7141 tgccaacttt taatttagcg cagagttatc agcaattact ccaaagacgt cagcaattag
     7201 cagacttgca aactcagtat caaactttga gtgatgaaaa ggataaggag acagcatttg
     7261 ctaccaagtt gaaagatgaa gattatgctg ctaaatatac acgagcgaag tactattatt
     7321 ctaagtcgag ggaaaaagtt tatacgattc ctgacttgct tcaaaggtga taaaatggaa
     7381 aatttattag acgtaataga gcaatttttg agtttgtcag atgaaaagct ggaagaattg
     7441 gctgataaaa atcaattatt gcgtttacaa gaagaaaagg aaaggaagaa tgcgtaaatt
     7501 cttaattatt ttgttgctac caagtttttt gaccatttca aaagtcgtta gcacagaaaa
     7561 agaagtcgtc tatacttcga aagaaattta ttacctttca caatctgact ttggtattta
     7621 ttttagagaa aaattaagtt ctcccatggt ttatggagag gttcctgttt atgcgaatga
     7681 agatttagta gtggaatctg ggaaattgac tcccaaaata agttttcaaa taaccgagtg
     7741 gcgcttaaat aaacaaggaa ttccagtatt taagctatca aatcatcaat ttatagctgc
     7801 ggacaaacga tttttatatg atcaatcaga ggtaactcca acaataaaaa aagtatggtt
     7861 agaatctgac tttaaactgt acaatagtcc ttatgattta aaagaagtga aatcatcctt
     7921 atcagcttat tcgcaagtat caatcgacaa gaccatgttt gtagaaggaa gagaatttct
     7981 acatattgat caggctggat gggtagctaa agaatcaact tctgaagaag ataatcggat
     8041 gagtaaagtt caagaaatgt tatctgaaaa atatcagaaa gattctttct ctatttatgt
     8101 taagcaactg actactggaa aagaagctgg tatcaatcaa gatgaaaaga tgtatgcagc
     8161 cagcgttttg aaactctcct atctctatta tacgcaagaa aaaataaatg agggtcttta
     8221 tcagttagat acgactgtaa aatacgtatc tgcagtcaat gattttccag gttcttataa
     8281 accagaggga agtggtagtc ttcctaaaaa agaagataat aaagaatatt ctttaaagga
     8341 tttaattacg aaagtatcaa aagaatctga taatgtagct cataatctat tgggatatta
     8401 catttcaaac caatctgatg ccacattcaa atccaaaatg tctgccatta tgggagatga
     8461 ttgggatcca aaagaaaaat tgatttcttc taagatggcc gggaagttta tggaagctat
     8521 ttataatcaa aatggatttg tgctagagtc tttgactaaa acagattttg atagtcagcg
     8581 aattgccaaa ggtgtttctg ttaaagtagc tcataaaatt ggagatgcgg atgaatttaa
     8641 gcatgatacg ggtgttgtct atgcagattc tccatttatt ctttctattt tcactaagaa
     8701 ttctgattat gatacgattt ctaagatagc caaggatgtt tatgaggttc taaaatgagg
     8761 gaacaagatt ttttaaatca ttttctcaag aagggatatt tcaaaaagca tgctaaggcg
     8821 gttctagctc tttctggtgg attagattct atgtttctat ttaaggtatt gtctacttat
     8881 caaaaagagt tagagattga attgattctg gctcatgtga atcataagca gagaattgaa
     8941 tcagattggg aagaaaagga attaaggaag ttggctgctg aagcagaact tcctatttat
     9001 atcagcaatt tttcaggaga attttcagaa gcgcgtgcac gaaattttcg ttatgatttt
     9061 tttcaagagg tcatgaaaaa gacaggtgcg acagctttag tcactgccca ccatgctgat
     9121 gatcaggtgg aaacgatttt gatgcgcttg attcgaggaa ctcgcttgcg ctatctatca
     9181 ggaattaagg agaagcaagt agtcggagag atagaaatca ttcgtccctt cttgcatttt
     9241 cagaaaaaag actttccatc aatttttcac tttgaagata catcaaatca ggagaatcat
     9301 tattttcgaa atcgtattcg aaactcttac ttaccagaat tggaaaaaga aaatcctcga
     9361 tttagggatg caatcttagg catcggcaat gaaattttag attatgattt ggcaatagct
     9421 gaattatcta acaatattga tgtggaaaat ttacagcagt tattttctta ctctgagtct
     9481 acacaaagag ttttacttca aacttatctg aatcgttttc cagatttgaa tcttacaaaa
     9541 gctcagtttg ctgaagttca gcagatttta aaatctaaaa gccagtatcg tcatccgatt
     9601 aaaaatggct atgaattgat aaaagagtac caacagtttc agatttgtaa aatcagtccg
     9661 caggctgatg aaaaggaaga tgaacttgtg ttacactatc aaaatcaggt agcttatcaa
     9721 ggatatttat tttcttttgg acttccatta gaaggtgaat taattcaaca aatacctgtt
     9781 tcacgtgaaa catccataca cattcgtcat cgaaaaacag gagatgtttt gattcaaaat
     9841 gggcatagaa aaaaactcag acgtttattt attgatttga aaatccctat ggaaaagaga
     9901 aactctgctc ttattattga gcaatttggt gaaattgtct caattttggg aattgcgacc
     9961 aataatttga gtaaaaaaac gaaaaatgat ataatgaaca ctgtacttta tatagaaaaa
    10021 atagataggt aaaaaatgtt agaaaacgat attaaaaaag tcctcgtttc acacgatgaa
    10081 attacagaag cagctaaaaa actaggtgct caattaacta aagactatgc aggaaaaaat
    10141 ccaatcttag ttgggatttt aaaaggatct attcctttta tggctgaatt ggtcaaacat
    10201 attgatacac atattgaaat ggacttcatg atggtttcta gctaccatgg tggaacagca
    10261 agtagtggtg ttatcaatat taaacaagat gtgactcaag atatcaaagg aagacatgtt
    10321 ctatttgtag aagatatcat tgatacaggt caaactttga agaatttgcg agatatgttt
    10381 aaagaaagag aagcagcttc tgttaaaatt gcaaccttgt tggataaacc agaaggacgt
    10441 gttgtagaaa ttgaggcaga ctatacctgc tttactatcc caaatgagtt tgtagtaggt
    10501 tatggtttag actacaaaga aaattatcgt aatcttcctt atattggagt attgaaagag
    10561 gaagtgtatt caaattagaa agaataatct ttaatgaaaa aacaaaataa tggtttaatt
    10621 aaaaatcctt ttctatggtt attatttatc tttttccttg tgacaggatt ccagtatttc
    10681 tattctggga ataactcagg aggaagtcag caaatcaact atactgagtt ggtacaagaa
    10741 attaccgatg gtaatgtaaa agaattaact taccaaccaa atggtagtgt tatcgaagtt
    10801 tctggtgtct ataaaaatcc taaaacaagt aaagaaggaa caggtattca gtttttcacg
    10861 ccatctgtta ctaaggtaga gaaatttacc agcactattc ttcctgcaga tactaccgta
    10921 tcagaattgc aaaaacttgc tactgaccat aaagcagaag taactgttaa gcatgaaagt
    10981 tcaagtggta tatggattaa tctactcgta tccattgtgc catttggaat tctattcttc
    11041 ttcctattct ctatgatggg aaatatggga ggaggcaatg gccgtaatcc aatgagtttt
    11101 ggacgtagta aggctaaagc agcaaataaa gaagatatta aagtaagatt ttcagatgtt
    11161 gctggagctg aggaagaaaa acaagaacta gttgaagttg ttgagttctt aaaagatcca
    11221 aaacgattca caaaacttgg agcccgtatt ccagcaggtg ttcttttgga gggacctccg
    11281 gggacaggta agactttgct tgctaaggca gtcgctggag aagcaggtgt tccattcttt
    11341 agtatctcag gttctgactt tgtagaaatg tttgtcggag ttggagctag tcgtgttcgc
    11401 tctctttttg aggatgccaa aaaagcagca ccagctatca tctttatcga tgaaattgat
    11461 gctgttggac gtcaacgtgg agttggtctc ggcggaggta atgacgaacg tgaacaaacc
    11521 ttgaaccaac ttttgattga gatggatggt tttgagggaa atgaagggat tatcgtcatc
    11581 gctgcgacaa accgttcaga tgtacttgat cctgcccttt tgcgtccagg acgttttgat
    11641 agaaaagtat tggttggccg tcctgatgtt aaaggtcgtg aagcaatctt gaaagttcac
    11701 gctaagaaca agcctttagc agaagatgtt gatttgaaat tagtggctca acaaactcca
    11761 ggctttgttg gtgctgattt agagaatgtc ttgaatgaag cagctttagt tgctgctcgt
    11821 cgcaataaat cgataattga tgcttcagat attgatgaag cagaagatag agttattgct
    11881 ggaccttcta agaaagataa gacagtttca caaaaagaac gagaattggt tgcttaccat
    11941 gaggcaggac ataccattgt tggtctagtc ttgtcgaatg ctcgcgttgt ccataaggtt
    12001 acaattgtac cacgcggccg tgcaggcgga tacatgattg cacttcctaa agaggatcaa
    12061 atgcttctat ctaaagaaga tatgaaagag caattggctg gcttaatggg tggacgtgta
    12121 gctgaagaaa ttatctttaa tgtccaaact acaggagctt caaacgactt tgaacaagcg
    12181 acacaaatgg cacgtgcaat ggttacagag tacggtatga gtgaaaaact tggcccagta
    12241 caatatgaag gaaaccatgc tatgtttggt gcacagaatc ctcaaaaatc aatttcagaa
    12301 caaacagctt atgaaattga tgaagaggtt cgttcattat taaatgaggc acgaaataaa
    12361 gctgctgaaa ttattcagtc aaatcgtgaa actcacaagt taattgcaga agcattattg
    12421 aaatacgaaa cattggatag tacacaaatt aaagctcttt acgaaacagg aaagatgcct
    12481 gaagcagtag aagaggaatc tcatgcacta tcctatgatg aagtaaagtc aaaaatgaat
    12541 gacgaaa
//
LOCUS       DAVKMU010000049        12378 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKMU010000049 DAVKMU010000000
VERSION     DAVKMU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12378
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.049"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..182
                     /locus_tag="VW343_001710"
     CDS             <1..182
                     /locus_tag="VW343_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9756231.1"
                     /translation="IVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIA
                     LNIKKERTKFVLSRA"
     gene            complement(268..1470)
                     /gene="rpsA"
                     /locus_tag="VW343_001711"
     CDS             complement(268..1470)
                     /gene="rpsA"
                     /locus_tag="VW343_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9756232.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRVARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1632..1703)
                     /locus_tag="VW343_001712"
     tRNA            complement(1632..1703)
                     /locus_tag="VW343_001712"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1669..1671),aa:Gln,seq:ttg)
     gene            complement(1713..1793)
                     /locus_tag="VW343_001713"
     tRNA            complement(1713..1793)
                     /locus_tag="VW343_001713"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1757..1759),aa:Tyr,seq:gta)
     gene            complement(1843..2073)
                     /locus_tag="VW343_001714"
     CDS             complement(1843..2073)
                     /locus_tag="VW343_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9756233.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2241..2885)
                     /gene="pcp"
                     /locus_tag="VW343_001715"
     CDS             complement(2241..2885)
                     /gene="pcp"
                     /locus_tag="VW343_001715"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9756234.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2900..3823)
                     /locus_tag="VW343_001716"
     CDS             complement(2900..3823)
                     /locus_tag="VW343_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9756235.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3820..4509)
                     /locus_tag="VW343_001717"
     CDS             complement(3820..4509)
                     /locus_tag="VW343_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9756236.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4574..5278)
                     /locus_tag="VW343_001718"
     CDS             complement(4574..5278)
                     /locus_tag="VW343_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9756237.1"
                     /translation="MVLSTLAILVACGKTDKEADAPTTFSYVYAVDPASLGYSITTRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPIVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(5334..6356)
                     /locus_tag="VW343_001719"
     CDS             complement(5334..6356)
                     /locus_tag="VW343_001719"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9756238.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6486..8957)
                     /gene="parC"
                     /locus_tag="VW343_001720"
     CDS             complement(6486..8957)
                     /gene="parC"
                     /locus_tag="VW343_001720"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9756239.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLRIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKNFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(9377..11320)
                     /gene="parE"
                     /locus_tag="VW343_001721"
     CDS             complement(9377..11320)
                     /gene="parE"
                     /locus_tag="VW343_001721"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9756240.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTALKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11456..12097
                     /gene="plsY"
                     /locus_tag="VW343_001722"
     CDS             11456..12097
                     /gene="plsY"
                     /locus_tag="VW343_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9756241.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(12140..>12378)
                     /locus_tag="VW343_001723"
     CDS             complement(12140..>12378)
                     /locus_tag="VW343_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9756242.1"
                     /translation="KGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSLNQL
                     EQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT         3567 a         2804 c         2171 g         3836 t
ORIGIN      
        1 agattgtcaa cgcccttcaa ctacactatt ctaatgccaa actggaagcg accaataatc
       61 tcatcaaact tatcaagcgc aatgcctttg gttttcggaa ctttgaaaac ttcaaaaaac
      121 ggattttcat cgctctgaat atcaaaaaag aaaggacgaa atttgtcctt tctcgagctt
      181 agcttttttt caacccacta cagttgacaa agagccagaa aaatcctttg tttagtaaac
      241 aaaggatttt gtgaattttc aatttgatta aagttcgata tcaccaaaca aatcagccat
      301 tgaaaatcct gtttgtgttt ctggaagttc gaaatcacgc ttttcttgac gtcttggacg
      361 acgtggacga gcaacacgtt tttcttcttt ttgtccttct tcttgggctg gacgttcttc
      421 aagagcttta atagaaagtg acacgcgttc tgcatctgcg ttaacttcaa gaactttaac
      481 ttgaacttct tgaccaactt taagagcttc ttttggattt tcaatccgtt tgtgtgaaat
      541 ttgtgatacg tgaacaagtc catcgatacc tggcaatact tcaacaaatg caccgaagtc
      601 agtcaaacgt ttaactgttc cttctactac atcaccttta gccaattttt gctcaacgcc
      661 atcccatggt cctggtactg ttgctttaag tgaaagtgat acacgtcctt cttcttcgtt
      721 aagatcaagg attttcactt caatttcttc accaacagtt acaactgatt ttggtgatac
      781 attacgttca tgtgacaatt cagtcaagtg aaccaatccg tcaacaccac caagatcgac
      841 gaaagcgccg aagcttgtga tacgagcaac tttaccagtt acaacatcac caacagccaa
      901 tttaccgaat acttcagcgc gagctgctgc agtagctgct tcaacaactt cacgacgtga
      961 aaggatgaag cggttttctt tagcgttaac ttctttgatt ttagtatcaa attcttgacc
     1021 tacaaaacgc tcagcgttac gtacgaaacg agtatccaac attgaagctg ggataaatcc
     1081 acgaacacct tcaaattcta ctgaaagtcc acctttaacg gcacgcgttc ctttaacagt
     1141 aacaacttct tcttcgcgac caacaagttt gtcccatgct ttgcgagctt caaggcgttt
     1201 tttagataca aggtatgtaa ctgtatcagt atctttacca actacttgac gaagtacaag
     1261 aacatccaat acttctccta ctttaacaaa gtcattgata tctgcatcac gatcgtttgt
     1321 caattcgcga agagtcaaga caccttcaac accagttcca gagattgcaa cgttagcttg
     1381 agtcgcatca actgtcaata cttcagcact aacaacatca ccagtctcaa cttgactaac
     1441 gctatttagc aaatcttcaa attcattcat ctaaaaaatc ctccaacaat caagtttttc
     1501 ttaaacttga catacttata atttttttcc taagcacccg caaggacatg ttcatatcat
     1561 tcacgtcttt caattataaa aataaaatac cctaagatat tttgcttttt atcttgatta
     1621 ttacctaaga actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg
     1681 ccttaccgct tggctatacc ccattaatga aatggagaga gagggattcg aacccccgaa
     1741 cccgaaggag cggatttaca gtccgccgcg tttagcctct tcgctatctc tcctacaatc
     1801 aacatggact attatatcat gaaaatttca aaaaaacaag acttattttg ctaaattata
     1861 attttcaatc aaaatttcca cagctttttc caatttttta taaaaactat cgacattccc
     1921 attctttata atggcaaatt gtccgtctaa ttcggcaatt tctccgataa cttttttacc
     1981 gatagtcaat gtataacctt caaaactgtc tttaccaaca ttaactttgg catccgctac
     2041 ttgaatttcg atttttttat ctttcttgct catttcatac ctctcttcac tttttctatt
     2101 ttacaagaaa atcctaaaac tagcaagaaa aaactctata tcaggcttct ctgatataga
     2161 gttttatgta aacaacctca atcgatttag catagttaat agttagtaaa caagcataat
     2221 tgactatcct ttttaggtta tcagtgagtc tcaccgccta ctaactgcaa atccttatct
     2281 ttgtagtcta caatagcttc aatagctacc tctatgcctc ttacaatatc acatagactc
     2341 attcctgcag tattcggctt atttactact tgttccatca tatatggaat atgcatgaaa
     2401 cctgctctca tattagggaa ttttttatcc actaaataaa gagcttggta catcaaatga
     2461 ttgcaaacaa aggttcctgc actattggat acaactgccg gaagtccctg ttttttgata
     2521 gcttgtacca tcgctttgat aggtaaacta ctaaaatagg ccgatgctcc atcaatacga
     2581 atcggtgtat caattggttg attgccttcg ttatcaggta tgcgagcatc atcttgatta
     2641 atagccactc gttcaggtgt taagccggtc ctgccgcctg cttgtccaat acaaagtaca
     2701 gcatctggtt gatatcgtaa tatttctgcc tctaaaactt ctgacgactt ataaaaaacc
     2761 gttggaattt ctacccagcg aacttcagcc ccattaatct cagatggtaa taattttaca
     2821 gcctccaaag ctggattaat cttttcacct ccaaaaggat taaaacctgt aaccaatatt
     2881 ttcattttat tttcctttac taaaatgcga gaaagtacat taagaatatg tgaataacaa
     2941 tcattactag agcaacacct gcttgagcct ttataacgcc attctgatct ttcatatcca
     3001 tcaatgctgc tggtagagcg ttaaaattag cagccattgg ggtcaataag gtcccacaat
     3061 aacctgctgt catggcaaga gcaccagcca caattggatt agctcccaga gcaaatacga
     3121 agggaactcc aacacctgct gtaataacgg tgaatgctgc aaaagcattt cccataatca
     3181 ttgtgaatag aaccattcca agaacatagg ccaaaactcc tataaagcga ctatctgaag
     3241 gaacaatacc gctaatcaga tgagagataa catcaccaac acctgctaca gtaaaaatag
     3301 cccccaaagc ccctaataat tgaggaacaa tcccacttgt tgaaacttgc tgagtcattc
     3361 gattattttc tgataacaga ctcttagggt gactattggt aatcacaaga acagaaattg
     3421 tagcaaacaa ggcggcaagg ctaatcgaaa tcttgctaaa ttctggaatc atttgcgcta
     3481 agaccaacgc aagtattgcc atcagcataa ctggaataaa aattttattt ttcaacctgt
     3541 tagattcaat attggctttc atttcatcta aggatggcaa ggttccgata cggacttgct
     3601 taaacaatgt taacagcgat aataggatta caataatacc aatactcata tttggcatat
     3661 aggaaccacc tataaacgta atagacaata gagtccaaaa tgcagatgtc ccaagtcgaa
     3721 ctgggtttgt tttatcttta taactacaat aggctgtatg gagaaattga caaccaatca
     3781 caatataggt caactctaat agttgctttg ccaactctgt catttttgtt ctcctcccct
     3841 agtctttttt gatatcaatt ttttatcaaa taaataattg taaatcccca ctacaataag
     3901 tgttataaca gcaacaataa tagatgtaga agcaatccct gcataattgc tttcatagcc
     3961 taactgatct aatgttcccc ctatcaagag gactccccca gcacctacaa acgtattttg
     4021 agcaaagaaa tttccaaaat tttcattcgc agccgcacgc gcttttattg tctcatcttc
     4081 aacctctgtt aactttctac ctaattgaga ctctgcagct gcttctccca taggttgaac
     4141 caaaggtctg acaaactgag ggtgtcctcc tagacgaatt gaaaagaaac cagctaactc
     4201 tcgaataaag aaataaactg tatagaagtt tccaactgtc agacctttaa tctttcgaat
     4261 caaatcgatt gatcgttgct tgagtccaaa ggtttctgac agccccacaa gaggcaaggt
     4321 aaccataaaa atcgtgagca ctcgctgatt gctaaattct tttcccaaaa tctccaaaaa
     4381 ttcaacgaga gaaacacctg aaactaaagc tgtaaccaaa ccagctaaga ctactgttgc
     4441 aattgtatca aattttaaaa taaaacccac aacaatgatt gctattccta ttaatctaat
     4501 ccactccata tcaaactcct ttatattcaa aatgacagta ttttaaaaat tttatcaaga
     4561 tcaataccat tccttattta atgtgttttt ctagttcttt ttggtatttg ctattggatt
     4621 ccaatttttc tttttgccat tttttaaaaa cctcgttata ttcttttgtt gtaacaatat
     4681 ctttttgcaa tttcattcct ttaaagatat atggttcccc cttaatacca acttgtgagt
     4741 atggttttga gaatggtact acgttactta caattggaga accaccagat gaagctgttg
     4801 gcatcaataa tgaactatct gtcgaccaag cttgagcttt ggcatatttt tcatatctct
     4861 tctctaggtc agtggtctca gaaacagcat cttctaacaa tttcttatat ttatccaaac
     4921 caggtttagc tacaacatcc ttatcttttc ctttcgtaat accaaggtgt ttcatggcag
     4981 aaccagattt tggatccata atattcaagt aagacgctgg atcttgatag cttggagccc
     5041 atcctgtact attcaaatca tagtcttttt gagaaggagc aacattgccg tatttatcat
     5101 tttccatcaa accatcaata acatttccaa taacgtctgt cgtcgatgtt cgagtcgtta
     5161 tactgtagcc caatgatgct ggatctactg catagacata agaaaatgtt gtcggtgcat
     5221 ctgcttcttt atcagttttt ccacaagcca ctaaaatagc taacgtgctc aggaccactc
     5281 ctgctgttaa gagccacttt ttctatttca taaaaaatct cctttggttt attttaatct
     5341 acttttacaa tccaaccttc tggcgcttca atatcgccaa actgaatacc cgtcaattca
     5401 ttatataatt tacgcgtcac aggacctact tctgtttcac tatagaatac atggaaatca
     5461 tcaccatgtt gaatacctcc aattggagaa ataaccgctg ctgtaccaca ggcacctgcc
     5521 tctacaaaac ggtcaagatt atcaattgga acatcaccct caataggagt taatcccaag
     5581 cgatgttctg ccaaataaag caaggaatac ttggtaatag atggcaagat agatggactc
     5641 aatggtgtta caaattcatt atcagctgta attccaaaga aattagctga tccgacttct
     5701 tcaatctttg tatgagttga tgggtccaga tagataacat ctgagaaatg gcgtgacttg
     5761 gccatttttc ctggtaagag acttgcagca tagtttccac caaccttagc cgcacctgta
     5821 ccatttggtg ctgcacggtc gtactcatcc tgaatcaaga agttggttgg gaccaaacca
     5881 cctttaaagt aatttccaac tggcatagca aagatggtga aaatgtactc ttctgccggt
     5941 tttaccccga taatatctcc gacaccaatc aaaagagggc gaagatataa ggttccacct
     6001 attccgtatg gtggtacgta ttcttcattc gcacggacaa ctgctttaca agcttctaca
     6061 aacatgtctg tcggaacttg tggcatcaag agacggtcac atgtacgttg caggcgttta
     6121 gcattttcat caggacggaa cagttgaaca ctgccatcct tagtacgata agctttcaaa
     6181 ccttcaaatg cttgttgtcc atagtgaaga cttggagaag actctgaaat atgcaaagtt
     6241 gcatcctctg taagctctcc ttgatcccat tgtccatttt tgaaatgagc aagatagcga
     6301 taaggtaatt tcatatagga aaaaccgagg ttttcccaat caatcgttac tgtcatattc
     6361 cactccttat tctaaaaacc tatttcttat attctacact atttttctaa aatagcaagt
     6421 atattttgta attttcagaa aattcctcca ataaaaacca tcttagaact gattcttcat
     6481 ttcacttatt tatcttcagt aactacttcc tgaagataag cgtcaaaaac ttcttcatct
     6541 gaaatcgtgt cagaaatgaa gcttccattg ctagtgcgtt ctgacaagtt caagtcttgc
     6601 aatcggcttt catagattgt tcctttattg gattggacaa gcagagtttg gtcgttcaca
     6661 tccacttccg tactgaagaa atcgccaaca aatccttgct ctgcaactgc tcctgccaag
     6721 aagacacgat gcggtttgtt tttcaactca cgcaagactt gtaatcctcg tttggcacgg
     6781 ctggttgcta gaatttcctc aatggaaaca cgtttcaagc ttccacgctg ggtcaagagg
     6841 tagaaggacg aagtattaca gataaagcca gattggagga catcatcttc tttcaaattc
     6901 atagccttga cacctgctgc cttagcaccg acaaccggaa cctcttcgat attgaaacgc
     6961 agggcataac cattttgact aaccaagaca acatcatcta gtttaatcgg agccactgct
     7021 acaatctgat ctgtatcgtc tttgagctta gcatacttga cagacttaga tctataggtc
     7081 cgccatggag tgaattcttt tcgctctacc cgtttgattt gaccaaggcg agtcgctgca
     7141 aagtaggttg tcgcatcgtc aaactgatcc agtacttcca cataaaggat tgcttcattc
     7201 gtttcaaagt ttgtgatggt ttggctcaga tgctctccga tgtccttcca acgaatatct
     7261 gccaactcat ggattggtct gtagatgaca tttccaagac ttgtgaacat caagaggtgc
     7321 tgggttgtct tggcagattg aacaaaaatc aaacggtcat catcacgctt gccaatttct
     7381 tccaaggtgg aagccgcaaa ggaacgtgga ctggtacgct tgatgtaacc tgccttggtc
     7441 acgctgacgt aggtatcttc ctcagcgata agactagctg tatcaatctc aattgctttc
     7501 gcagtgtctt ctaaagaact caaacgagga gttgcaaagt tcttcttgac ctcacgaagt
     7561 tctttcttca tgagattgta catagtcctt tcatcaccga taatagccgc cagcatagca
     7621 atcttctcac gaagctctgc ttcttcttcc tgcaagacaa ccacatcggt attggtcaaa
     7681 cggtacagtt gcaaagttac gatagcctca gcctgttctt ccgtaaaatc atagctaact
     7741 ttgaggtttt ccttggcgtc cgccttattc tcagaagcac ggataagagc aatgacttca
     7801 tccaaaatcg aaatcacacg aatcaaacct tcgacgatac ggagacgttt ctcagccttt
     7861 tctttgtcaa agcgtgaacg cgccaaaatc acttctcgac ggtgagcgat gtagctagac
     7921 aggattggaa caatcccaac ctgacgaggt gtgaaattgt caatcgccac catattaaag
     7981 ttgtagttga tttgtaggtc ggtgtactta aataagtagt tgagaacaag ctcagtatta
     8041 gcgtctttct taagttcaat agcgatacga agaccatcac ggtcagactc atcacgaacc
     8101 tcagcaatcc cagctacctt gttattaaca cgaacatcat cgattttctt gactagattg
     8161 gccttattga tttcataagg aatctcagta ataacgattt gttccttacc accttttagc
     8221 ttttcaattt cagtcttgga acgaacaacc acgcgccctt tcccagtctc ataggctttc
     8281 ttgatttcat cacgaccctg aataatagcc cctgtaggga agtctggtcc aggtaagaat
     8341 tccatgagtt tatcaatctt tgcagttggg tggtcaatca tgtaaactgc agcatctatg
     8401 acctcagcta aattatgggg aggaatgtct gtggcataac cagccgaaat cccagtcgaa
     8461 ccattgacca agaggtttgg aaaggctgct ggcaagaccg ttggttcttt ctccgtatcg
     8521 tcaaagttcc atgcaaaagg aactgtcttt ttctcgatat cctgaagaag gtagcctgca
     8581 atttcagaca aacgtgcctc agtataacgc atagccgcag gaggatctcc gtccatagaa
     8641 ccgttattac cgtgcatttc aactagaatc tcacgatttt tccagttctg tgacatacga
     8701 accatggcat catagataga agaatccccg tgtgggtgga aattccccat gatgttcccg
     8761 actgacttgg ccgacttacg gtagctcttg tcaaaagtat tgctatcctt attcatagaa
     8821 taaagaatac ggcgttgaac cggcttcaac ccatcacgaa tatctggcaa agcccggtct
     8881 tgaataatgt acttggagta gcgaccaaag cgctctccca tgatgtcctc cagggacatg
     8941 ttttgaatgt tagacataag atacaaagcc cataaaatac caagtgaaaa tagaaaattc
     9001 ttgaagtaag caaactcaca agagaattta tctttttcac acagtatcta gggcgtgttc
     9061 aactcctttc aaagaatgta gagtaggttt ttatgcagta aaagatattt tacgggaatt
     9121 cgtcccgtgt tcagttacga taagtaacca aactatcctg tttgtatttt tcaatatgaa
     9181 aatctggttt tccaaaatta gtcttagttt gtgtcttaac cgctccctta agcgcctctt
     9241 taagataagc actcatagca gattcttcat taataatcct gcaatttttt caaaccaaga
     9301 ttttcaaact gctttttcac atagtcattc acatccgact ctaatttcca gtttactaac
     9361 atattatttt ctttcattaa aacactgtcg cttcttctag cgtaaacttg acattatctt
     9421 caatccattt acggcgtggt tctaccttat ctcccatgag aacattgacg cggcgttcgg
     9481 cgcgcgctaa atcttcaatt gtgacacgga tgagggtacg tgtttctggg ttcatggttg
     9541 tttcccagag ctggtccgca ttcatctcac caagtccttt gtatcgttgg agggtagcgc
     9601 ctttaccgaa ctgtttacgg agttcttcta gttctccgtc cgtccaagcg taggccactt
     9661 cttctttctt gcctttacct ttggacatct tgtaaagagg tgggagggca atatagacat
     9721 gacctgcctc gactagcgga cgcatgtaac ggtagaaaaa tgtcaagagc aaggtctgga
     9781 tatgggcacc gtcggtatcc gcatcggtca tgataatgat cttatcatag ttggcatctt
     9841 caatagagaa gtctgctcca acacccgcac caatggtata aatcatggta ttgatctctt
     9901 catttttgag gatatccgcc atcttggcct tggctgtatt gataacctta ccacgaagag
     9961 gtagaatagc ctggaacttg cggtcacgac cttgtttggc agaaccaccg gcagagtccc
    10021 cctcaactag atagagttca ttcttagcag gattcttaga ttgggctggg gtcaatttcc
    10081 cagacaacaa gcccttatct ttcttgtttt tcttcccatt tcggctctca tcacgcgcct
    10141 tacgtgctgc ttcacgagca tcacgggcct tgatagcctt gcggatgagg ttagaagcta
    10201 attccccatt ttccataagg aaaaaggtca acttatcagc cactattcca tccacaactg
    10261 ggcgagctag ggggcttcct agtttatcct tggtctgtcc ttcaaactgc aagtgttctt
    10321 caggaactaa gatagaaaga acggccgcta gtccctcacg atagtctgaa ccttcaaggt
    10381 ttttatcttt ttccttgaga agacctgttt tacgtgcata gtcattcatg accttggtaa
    10441 tggcagactt gagtcctgtc tcgtgcgttc caccgtcctt ggtgcgaacg ttattgacaa
    10501 aggatagaat gttatctgag aatccgtcat tgtactggag ggctacttcc acttgaaaac
    10561 cattgtcttc cccttcaaag taaagaactg gcgtcaagat ttccttatct tcgttgagat
    10621 aagaaacaaa atcttgtact ccattctcat agtggaactc aatcgcttca tctgttcgct
    10681 tgtccgttaa agacaaggtc acatttttca agagaaaggc tgattcatta aggcgctctg
    10741 aaatggtatt gtacttgaaa tctgtcgtag aaaagatagt cgcgtcaggc ataaaagtaa
    10801 ctttggtgcc tgttttagac ttgagtgctg taccgatttt cttcaaagtc gtgacaggtt
    10861 ttccaccatt ttcaaaacgt tgcttgtaaa ctgcgccatc acgggtaatt tcaacttcta
    10921 accagctaga aagggcgtta acaacggaag aacccactcc gtgaagtcca cctgatgtct
    10981 tatagccacc ttgaccgaat ttccctccgg catgaagaat ggtaaagata acctcaacag
    11041 ttggaattcc catagcgtgc atacctgtcg gcatcccacg tccatggtct tgaaccgtta
    11101 gactaccgtc tttattgata gttacatcaa tacgatcacc aaacccagac aaggcttcat
    11161 cgactgcatt atcaacgatt tcccaaacta ggtgatgaag accagcgcca tcggtcgatc
    11221 caatatacat ccctggacgt tttcggaccg catccaaccc ttctagcacc tgaatagcat
    11281 catcattata attgttaata ttgatttcct tttttgacac aaggaacctc ctattcgttc
    11341 atctttacta ttctacaggt tttccaagga ttttgcaaaa tttttctttc tccgatgtga
    11401 caatttcagc agagattctc tgcttttctt tcccaattca tgatataata ggagtatgat
    11461 tacaatagtt ttattaatcc tagcctatct gctgggttcg attccatctg gtctctggat
    11521 tggacaagta ttctttcaaa tcaatctacg cgagcatggt tctggtaaca ctggaacgac
    11581 caacaccttc cgcattttag gtaagaaagc tggtatggca acctttgtga ttgacttttt
    11641 caaaggaacc ctagcaacgc tgcttccgat tatttttcat ctacaaggcg tttctcctct
    11701 catctttgga cttttggctg ttatcggcca taccttccct atctttgcag gatttaaagg
    11761 tggtaaggct gtcgcaacca gtgctggagt gattttcgga tttgcgccta tcttctgtct
    11821 ctaccttgcg attatcttct ttggagctct ctatcttggc agtatgattt cactgtctag
    11881 tgtcacagca tcgatcgcgg ctgttatcgg ggttctgctc tttccacttt ttggttttat
    11941 cctgagtaac tatgactctc tcttcatcgc tattatctta gcacttgcta gtttgattat
    12001 cattcgtcat aaggacaata tagctcgtat caaaaataaa actgaaaatt tggtcccttg
    12061 gggattgaac ctaacccatc aagatcctaa aaaataaaac gccagttctg tactgccccc
    12121 aaacagttag acaaataatt tatccaaagg atttagttct gtattgcaca gggctaagtc
    12181 cttttagttt taccttaatt cgtttgttgt tgtagtaatc aatatagtct ataatggctt
    12241 gttccaattg gttaagtgat ttaaatgttt tctcatagcc ataaaacatt tcggatttca
    12301 gaatcccaaa gaaggactcc atcataccgt tgtcttggct gtttcccttg cgtgacatag
    12361 atgcttgaat tcccttac
//
LOCUS       DAVKMU010000050        12337 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKMU010000050 DAVKMU010000000
VERSION     DAVKMU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.050"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(171..1802)
                     /locus_tag="VW343_001724"
     CDS             complement(171..1802)
                     /locus_tag="VW343_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9756243.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNIIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2095..7074
                     /locus_tag="VW343_001725"
     CDS             2095..7074
                     /locus_tag="VW343_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9756244.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELTFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7164..8360
                     /locus_tag="VW343_001726"
     CDS             7164..8360
                     /locus_tag="VW343_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9756245.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8496..9023
                     /locus_tag="VW343_001727"
     CDS             8496..9023
                     /locus_tag="VW343_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9756246.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRVRLFLEKKEK"
     gene            9100..9456
                     /gene="glnR"
                     /locus_tag="VW343_001728"
     CDS             9100..9456
                     /gene="glnR"
                     /locus_tag="VW343_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9756247.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9493..10839
                     /gene="glnA"
                     /locus_tag="VW343_001729"
     CDS             9493..10839
                     /gene="glnA"
                     /locus_tag="VW343_001729"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9756248.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10935..11054)
                     /locus_tag="VW343_001730"
     CDS             complement(10935..11054)
                     /locus_tag="VW343_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9756249.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11312..11449
                     /locus_tag="VW343_001731"
     CDS             11312..11449
                     /locus_tag="VW343_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756250.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
     gene            11542..12337
                     /locus_tag="VW343_001732"
                     /pseudo
     CDS             11542..12337
                     /locus_tag="VW343_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3996 a         2237 c         2643 g         3461 t
ORIGIN      
        1 aaataataac aggacaaatc gatttctaac aatgttttag aaatagaggt gtactattct
       61 agtttcaata tactatatat ctgcagaata ctctttcagt ttcattcagc ctagtcttgc
      121 ttaggaaaaa gtaagaagcc atccaattat gatggatagc ttcttggtta ttagatttgt
      181 tctgcaaaaa ccttttcagc aaggttcata gcgtggtctg atacacgagt gtagtgtgag
      241 atgatgtcga taaagttgac cccagcttgt gttgaacatt cgcctttgtt gaggcgtttg
      301 atgtgggttt ttctgagaac acgttctatc ttattgattg cttcatgacg ttctacaaga
      361 ctgcgtgctt tttcaatatc attgttttcc acactatcca gagcatcttt gataaagtca
      421 ctagtttggc ggtaaacttc ctctaattct ttcaaggcgg catcagaaaa ttcaacattt
      481 ttccgttgaa gatagtcagt cagattgagt agagcctccg tgtggtctcc aatccgttcc
      541 aaatcacggg aggaatcaag gatattggta agcacttcac tttctttttg gctgagagct
      601 tcgcttgaaa gggcaatgag ataacgtgtt aattgctcat cgatggtgtt aattgcttct
      661 tcggttttat gccctttttc agcaactttt tcatccaagt caatgatgta cttataggaa
      721 aggtcaaagg ctttagcagc gtagtttcct aagtgcaaga gctctttctt agcatttcct
      781 agagcgatag atggggcctg tttgatgaaa tgttcatcaa gatataaggg ttcgtatttg
      841 acaacctcgt cctctccagg aataatcttg gttacaaagt aagccagagc tccgataaat
      901 ggaaattgga caatggtgtt ggtaatatta aaggttccgt gagcaaaggc gatggtcatt
      961 tccggtgcta gatttagcgt agcttcaaac caatgaatca ggacagtaaa aggaactaga
     1021 aaaataacgc agacgactgt tccgataatg ttgaaggcaa catgagctcc tgctacccgt
     1081 ttagctgcaa tattagcccc taaagaggca atgatggctg taatggttgt cccgatattg
     1141 tcaccaaata gaactggcaa agctccctgt aggtcaatta gattgccggc gtagaggttt
     1201 tgtaaaatcc caatggtagc cgaagaagct tgaattagca aggtcaagcc agtaccgaca
     1261 aagacaccca aaacaggatt cttacttagc tcaatcatat agtccttaaa gacctgtaaa
     1321 tccttgagtg gagccattgc gccgctcatg agattgaggg caaaaaagat accaccgata
     1381 ccaaagagga tgcgtccgat attattgact gtccgatttt tcgtaaaaaa gagacagacg
     1441 gcaccgataa agagcatagg tagggcatag ttacctaatt taaaaccgat gagaaaggat
     1501 gtgacagttg tcccaatatt agcacccatg acaatcccga tagcctgacg taaggttaag
     1561 agaccggcac tgaccaggcc gactgtaata actgttacac cagaactaga ctgaattaga
     1621 gcagtcatcc caataccaac cagaactcca aagaaaggat tactagtata tttgtcaatg
     1681 taaaaacgaa ggcgatctcc agcagcttgt tgcaaaccgt ctcccatggt cttgatgcta
     1741 tataagaata gccccagacc acctaaaaag tgaaataaaa tttcctgcca attaatggac
     1801 atttcttttt cctccgaaaa ataatcgcgg aatatctcct attctatttt aaaggataaa
     1861 agtaaatcta acaagtgtta acgtcaagtt tgtaaaagaa aattataatt gataagtgta
     1921 ttatttttaa gagtttttaa ccttatttta gatttatgaa agcagctatt gttttttgtc
     1981 taaaaatgtt ttaggataaa aatgtgatta tcatggttct agagtttgat aaaaaactac
     2041 agaatataaa gcgctttctt gaaaacaaca aaatcaatct tttaggagga gaaaatgaag
     2101 aatccatttt ttgaaagacg ttgtcgttac agtattcgta agttatcagt aggagcctgc
     2161 tcgctgatga ttggtgctgt tttatttgct ggtccagcct tggctgaaga aactgcagtt
     2221 cctgaaaata gcggagctaa tacagagctt gtttcaggag agagtgagca ttcgaccaat
     2281 gaagctgata agcagaatga aggggaacat gctagagaaa acaagctaga aaaggcagaa
     2341 ggagtagcga tagcatctga aactgcttcg ccagcaagca atgaagctgc aactactgaa
     2401 actgcagaag cagctagcgc agctaaacca gaggaaaaag caagtgaggt ggttgcagaa
     2461 acaccatctg cagaagcaaa acctaagtct gacaaggaaa cagaagcaaa gcccgaagca
     2521 actaaccaag gggatgagtc taaaccagca gcagaagcta ataagactga aaaagaagtc
     2581 cagccagatg tccctaaaaa tacagaaaaa acattaaaac caaaggaaat caaatttaat
     2641 tcttgggaag aattgttaaa atgggaacca ggtgctcgtg aagacgatgc tattaaccgc
     2701 ggatctgttg tcctcgcttc acgtcggaca ggtcatttag tcaatgaaaa agctagcaag
     2761 gaagcaaaag ttcaagcctt atcaaacacc aattctaaag caaaagacca tgcttctgtt
     2821 ggtggagaag agttcaaggc ctatgctttt gactattggc aatatctaga ttcaatggtc
     2881 ttctgggaag gtctcgtacc aactcctgac gttattgatg caggtcaccg taacggggtt
     2941 cctgtatacg gtacactctt cttcaactgg tctaatagta ttgcagatca agaaagattt
     3001 gctgaagctt tgaagcaaga cgcagatggt agcttcccaa ttgcccgtaa attggtagac
     3061 atggcaaagt attatggcta tgatggctat ttcatcaacc aagaaacaac tggagatttg
     3121 gttaaacctc ttggagaaaa gatgcgccag tttatgctct atagcaagga atatgctgct
     3181 aaggtaaacc atccaatcaa gtattcttgg tatgatgcca tgacctataa ctatggacgt
     3241 taccatcaag atggtttggg agaatacaac taccaattca tgcaaccaga aggagataag
     3301 gttccggcag ataacttctt tgctaacttt aactgggata aggctaaaaa tgattacact
     3361 attgcaactg ccaactggat tggtcgtaat ccttatgatg tatttgcagg tttggaattg
     3421 caacagggtg gttcctacaa gacaaaggtt aagtggaatg acattttaga cgaaaatggg
     3481 aaattgcgcc tttctcttgg tttatttgcc ccagatacca ttacaagttt aggaaaaact
     3541 ggtgaagatt atcataaaaa tgaagatatc ttctttacag gttatcaagg agaccccact
     3601 ggccaaaaac caggtgacaa agattggtat ggtattgcta acctagttgc ggaccgtacg
     3661 ccagcggtag gtaatacttt tactacttct tttaatacag gtcatggtaa aaaatggttc
     3721 gtagatggta aggtttctaa ggattctgag tggaattatc gttcagtatc aggtgttctt
     3781 ccaacatggc gctggtggca gacttcaaca ggggaaaaac ttcgtgcaga atatgatttt
     3841 acagatgcct ataatggcgg aaattccctt aaattctctg gtgatgtagc tggtaagaca
     3901 gatcaggatg tgagacttta ttctactaag ttagaagtaa ctgagaagac caaacttcgt
     3961 gttgcccaca agggaggaaa aggttctaaa gtttatatgg cattctctac aactccagac
     4021 tacaaattcg atgatgcaga tgcatggaaa gagctaaccc tttctgacaa ctggacaaat
     4081 gaagaatttg atcttagctc actagcgggt aaaaccatct atgcagtcaa actatttttc
     4141 gagcatgaag gtgctgtaaa agattatcag tttaacctag gacaattaac tatctcggac
     4201 aatcaccaag agccacaatc gccgacaagc ttttctgtag tgaaacaatc tcttaaaaat
     4261 gcccaagaag cggaagcagt tgtgcaattt aaaggcaaca aggatgcaga tttctatgaa
     4321 gtttatgaaa aagatggaga cagctggaaa ttactaactg gctcatcttc tacaactatt
     4381 tatctaccaa aagttagccg ctcagcaagt gctcagggta caactcaaga actgaaggtt
     4441 gtagcagtcg gtaaaaatgg agttcgttca gaagctgcaa ccacaacctt tgattggggt
     4501 atgactgtaa aagataccag cctaccaaaa ccactagctg aaaatatcgt tccaggtgca
     4561 acagttattg atagtacttt ccctaagact gaaggtggag aaggtattga aggtatgttg
     4621 aacggtacca ttactagctt gtcagataaa tggtcttcag ctcagttgag tggtagtgtg
     4681 gatattcgtt tgaccaagcc acgtaccgtt gttagatggg tcatggatca tgcaggagct
     4741 ggtggtgagt ctgttaacga tggcttgatg aacactaaag actttgacct ttattataaa
     4801 gatgcagatg gtgagtggaa gctagctaag gaagtccgtg gtaacaaagc acacgtgaca
     4861 gatatcactc ttgataaacc aatcactgct caagactggc gcttgaatgt tgtcacttct
     4921 gacaatggaa ctccatggaa ggctattcgt atctataact ggaaaatgta tgaaaagctt
     4981 gatactgaga gtgtcaatat tccgatggcc aaggctgcag cccgttctct aggcaataac
     5041 aaggtacaag ttggctttgc agatgtaccg gctggagcaa ctattaccgt ttatgataat
     5101 ccaaattctc aaactccgct cgcaaccttg aagagcgaag ttggaggaga cctagcaagt
     5161 gcaccattgg atttgacaaa tcaatctggt cttctttatt atcgtaccca gttgccaggc
     5221 aaggaaatta gtaatgtcct agcagtttcc gttccaaaag atgacagaag aatcaagtca
     5281 gtcagcctag aaacaggacc taagaaaaca agctacgccg aaggggagga tttggacctt
     5341 agaggtggtg ttcttcgagt tcagtatgaa ggaggaactg aggacgaact cattcgccta
     5401 actcacgcag gtgtatcagt atcaggtttt gatacgcatc ataagggaga acagaatctt
     5461 actctccaat atttgggaca accggtaaat gctaatttgt cagtgactgt cactggccaa
     5521 gacgaagcaa gtccgaaaac tattttggga attgaagtaa gtcaggaacc gaaaaaagat
     5581 tacctagttg gtgatagctt agacttgtct gaaggacgct ttgcagtggc ttatagcaat
     5641 gacaccatgg aagaacattc ctttactgat gagggagttg aaatttctgg ttacgatgct
     5701 caaaagactg gtcgtcaaac cttgacgctt cattaccaag gccatgaagt tagctttgat
     5761 gttttggtat ctccaaaagc agcattgaac gatgagtacc tcaaacaaaa attagcagaa
     5821 gttgaagctg ctaagaacaa ggtggtctat aactttgctt catcagaagt aaaagaagcc
     5881 ttcttgaaag caattgaagc ggccgaacaa gtgttgaaag accatgaaac tagcacccaa
     5941 gatcaagtca atgaccgact taataaattg acagaagctc ataaagctct gaatggtcaa
     6001 gagaaattta cggaagaaaa gacagagctt gatcgcttaa caggtgaggt tcaagaactc
     6061 ttggctgcca aaccaaacca tccttcaggt tctgccctag ctccgcttct tgagaaaaac
     6121 aaggccttgg ttgaaaaagt agatttgagt ccagaagagc ttacaacagc gaaacagagt
     6181 ctaaaagatc tggttgcttt attgaaagaa gacaagccag cagtcttttc tgatagtaaa
     6241 acaggtgttg aagtacactt ctcaaataaa gagaagactg tcatcaaggg tttgaaagta
     6301 gagcgtgttc aagcaagtgc tgaagagaag aaatactttg ctggagaaga tgctcatgtc
     6361 tttgaaatag aaggtttgga tgaaaaaggt caagatgttg atctctctta cgcttctatt
     6421 gtgaaaatcc caattgaaaa agataagaaa gttaagaaag tatttttctt acctgaaggc
     6481 aaagaggcag tagaattgac ttttgaacaa acggatagtc atgttatctt tacagcacca
     6541 cactttactc attatgcctt tgtttatgaa tctgctgaaa aaccacaacc tgctaaacca
     6601 gcaccacaaa acaaagtcct tccaaaacct acttatcaac cgacttctga tcaacaaaag
     6661 gctcctaaat tggaagttca agaggaaaag gttgcctttc atcgtcaaga gcatgaaaat
     6721 gctgagatgc tagttgggga acaacgagtc atcatacagg gacgagatgg actgttaaga
     6781 catgtctttg aagttgatga aaacggtcag cgtcgtcttc gttcaacaga agtcatccaa
     6841 gaagcgattc cagaaattgt tgaaattgga acaaaagtaa aaacagtacc agcagtagta
     6901 gctacacagg aaaaaccagc tcaaaataca gcagttaaat cagaagaagc aagcaaacaa
     6961 ttgccaaata caggaacagc tgatgctaat gaagccctaa tagcaggctt agccagcctc
     7021 ggtcttgcta gtttagcctt gaccttgaga cggaaaagag aagataaaga ttaaatatcg
     7081 aaaaatcttg tgaaatcttt ccttatattt ccaaagtgtg atataatagt ttcgaataaa
     7141 ataaataaag gagtttttgt aacatggcaa aacttactgt taaagacgtt gacttgaaag
     7201 gtaaaaaagt cctcgttcgt gttgacttca acgtaccatt gaaagatggc gtaatcacta
     7261 acgataaccg tatcacagca gctcttccaa ctattaagta catcatcgaa caaggtggac
     7321 gtgcaattct tttctctcac cttggacgtg tgaaagaaga agctgataaa gctggtaaat
     7381 cacttgctcc tgtagcagct gacttggcag caaaacttgg tcaagatgtt gttttcccag
     7441 gtgtcactcg tggtgctgaa ttggaagcgg caatcaacgc tcttgaagat ggacaagttc
     7501 tcttggttga aaacactcgt tacgaagatg ttgacggcaa gaaagaatct aaaaacgatc
     7561 ctgaacttgg taaatactgg gcatcacttg gagatggtat cttcgtaaac gatgcattcg
     7621 gtacagctca ccgtgcacac gcatctaacg ttggtatctc agcaaacgtt gaaaaagcag
     7681 ttgctggttt ccttcttgaa aacgaaattg cctacatcca agaagcagtt gaaactccag
     7741 aacgtccatt cgtggctatc cttggtggtt caaaagtttc agacaagatc ggtgttatcg
     7801 aaaacttgct tgaaaaagct gataaagtcc ttatcggtgg tgggatgact tacacattct
     7861 acaaagcaca aggtatcgaa atcggtaact cacttgtaga agaagacaaa ttggatgttg
     7921 cgaaagctct tcttgaaaaa gcaaatggta aattgatctt gccagttgac tcaaaagaag
     7981 ctaacgcatt tgctggttac actgaagtgc gtgacactga aggtgaagca gtttctgaag
     8041 gcttccttgg tcttgacatc ggtccaaaat ctatcgccaa atttgacgaa gctttgactg
     8101 gtgccaaaac agttgtatgg aacggaccta tgggtgtatt tgaaaaccca gatttccaag
     8161 ctggtacaat cggtgtgatg gacgctatcg tgaaacaacc aggagttaaa tcaatcatcg
     8221 gtggtggtga ctcagctgcc gcagcgatta accttggccg tgcagacaag ttctcatgga
     8281 ttagtacggg tggtggagca tcaatggaac ttcttgaagg taaggttctt ccaggacttg
     8341 cagccctgac agaaaaataa gattttataa acaaatcaaa gaagagaggg atgaaagttc
     8401 ctcttttctt ttgcttaaaa taaaaacgct tcctctcaac tattactcat aaaaatcacc
     8461 gatttatgat aaaatggaaa tagaaagttg agattatgag ttattttaaa aaatataaat
     8521 tcgataaatc ccagttcaaa cttggtatgc gaacctttaa aacaggtatt gctgtttttc
     8581 tagttctctt gatttttggc ttttttggct ggaaaggtct tcaaattggt gctttgacag
     8641 ccgtttttag cctgagggag agttttgatg agagtgttca ttttgggact tcgcgtattc
     8701 taggaaatag tatcggtgga ctctatgcct tggtcttctt cttattaaat acctttttcc
     8761 acgaagcctt ttgggtgacc ttggtagttg ttccaatctg caccatgtta accattatga
     8821 caaatgtagc catgaataac aaagcagggg ttattggtgg tgtagcagct atgttaatca
     8881 ttaccctatc aattccaagt ggtgagacaa tttcgtacgt gtttgtgcgt gtatcagaaa
     8941 cgtttatggg agtttttgtc gcaattatcg taaattacga tattgatcgt gttcgtctct
     9001 ttttagagaa aaaagaaaaa taatgttaca ttttataaca ttatcaattg acgtttgtct
     9061 ttttttagac tataatagac agaaagaagg aaattgtaaa tgaaggaaaa agaatttcgc
     9121 cgaaatatgg ctgtttttcc tatcggcagt gttatgaagt tgaccgatct atcggcgcgt
     9181 cagattcgtt attatgaaga tcaagagttg atcaagcccg atcgaaacga aggaaatcgt
     9241 cgcatgtatt ccttgaatga catggatcgt ctgcttgaaa tcaaagatta tatctctgaa
     9301 ggttataata tcgctgccat taagaaaaaa tatgctgaac gtgaagcgaa atccaagaaa
     9361 gcggttagtc agactgaagt acgtcgtgca cttcacaatg aactcctcca acaggggcgt
     9421 tttgcttcag tacagtcacc ttttggtcgc ggttaggcaa ccgcaagtag tcatagatta
     9481 aggagaaaac ttatgccaat cacagctgca gatattcgtc gtgaagtcaa ggaaaaaaat
     9541 gttaccttta ttcgtcttat gttctcagat attttgggaa ccatgaaaaa cgtcgaaatt
     9601 cctgctacag atgaacagtt agataaggtt ttgtcgaaca aggttatgtt tgatggatct
     9661 tctattgaag gttttgtacg tatcaatgag tcggatatgt acttgtaccc ggacttggat
     9721 acatggacag tcttcccttg gggagatgaa aatggaagtg ttgcaggtct gatctgtgat
     9781 gtctatacaa cagaaggtga accatttgcg ggtgaccctc gtggtaattt gaaacgagct
     9841 cttcgtcaca tggaagaagt tggattcaaa tccttcaacc ttggtccaga gccagaattc
     9901 ttcctattta agttggatga aaatggggac ccaacacttg aagtgaatga caagggtggc
     9961 tactttgatt tggcacctac tgaccttgcg gacaacacac gtcgtgagat tgtgaatgtc
    10021 ttgaccaaaa tgggatttga agtagaagcg agtcaccacg aggttgcggt tggacagcat
    10081 gagattgact ttaagtacga tgaagttctc cgtgcttgtg ataagattca aatctttaaa
    10141 cttgttgtta aaaccattgc tcgcaaacac ggactttacg caacatttat ggcgaagcca
    10201 aaatttggta ttgctggctc aggtatgcac tgtaatatgt ccttgtttga tgcagaagga
    10261 aataacgcct tctttgatcc aaatgatcca aaaggaatgc agttgtcaga aacagcttac
    10321 catttcctag gcggtttgat caagcatgct tacaactata ctgccatcat gaacccaaca
    10381 gttaactcat acaaacgttt ggttccaggt tatgaagcgc ctgtttacat tgcttgggct
    10441 ggtcgtaacc gttcgccact tgtgcgcgta cctgcttcac gtggtatggg aactcgtctt
    10501 gagttgcgtt cagtggatcc aatggcgaac ccttacattg ctatggctgt tcttttggaa
    10561 gttggtttgt atggtattga aaataaaatc gaagcaccag ctcctatcga agaaaatatc
    10621 tacatcatga cagcagaaga gcgcaaggaa gctggtatta cagaccttcc atcaactctt
    10681 cacaacgctt tgaaagcttt gacagaagat gaagtggtta aagctgctct cggagatcac
    10741 atctacacta gcttccttga agccaaacga atcgaatggg caagttatgc aaccttcgtt
    10801 tcacaatggg aaattgataa ttatttagac ctttactaat actaatatag aagaaagatt
    10861 gcctgtgggt aaacagtcac acagcagtct aagccatcaa atacctataa cccttaaaat
    10921 agcgatttcg gtcgctattt atttttcgaa aaatcctctt gactcttttg cgaattagat
    10981 ttatcatcag acgtgtagaa gaaaattaag gagtcactat ggaaaaattc aatttcaaaa
    11041 acaatatcgg gcaagaaaac aagttacttc aaatcgaaat ctacaagttt actaactttt
    11101 gcaagcttca aaattacaca tctgtaaata ttttttcgaa agatatattt gaagccatag
    11161 taaattgaga ctagaatggt acactacatc tgctaaaaat ttctagaaac taatttaact
    11221 ttcctaatcg atttgtttac atcttatttc aatctactat attatctcaa taaatgatga
    11281 tggcgactgt tttgtttgtg cttgtcgatt tttgaatcct gatcgagcgg aagaatattt
    11341 ctgccgaggt tgccaagggg aaaatcctga agatattgaa ttttatgatg aacaattaca
    11401 ggcagaaaag gtggaggttt tgaatattcg attggaagta aaaaattgaa ttatctacaa
    11461 tctcatcaac aataaaccta tttcaacccc attatctata ggcgtatttt tacgcctttt
    11521 tatatacttg aagcacataa gaaacattat gggaagtttg ctgatggaag tactcaagaa
    11581 attgatgttc cttatgatat tcctgatact tgggagtggg tgaggttttc tacattggtt
    11641 gaaattgtca gaggtggctc tccacgacca atcaaagatt atcttacttc tgaagtagat
    11701 ggaataaatt ggataaaaat aggtgatact gaaaagggtg aaaagtatat aaataatgtt
    11761 aaagaaaaaa tcaaaaaatc agggcttaac aaaactagat ttgtaaaaaa aggtacattt
    11821 ttgttaacta attctatgag ttttggtaga ccttatattt tgaatgttga tggtgcaata
    11881 cacgatggat ggttggctat ttcgaactat gaaaactcat taaataaaga ttacctattc
    11941 tatattcttt catcaaatgt agtttattct caatttctat ctctaattag tggagctgtt
    12001 gtgaaaaact tgaatagtga taaagttgct tctattctta tccctctccc cccactatcc
    12061 gaacaacaac gcataataga agcaatcgaa tcagctttag aaaaagtaga tgaatatgct
    12121 gaaagttata atagactaga acagctagat aaagaatttc cagataaact aaaaaaatct
    12181 attcttcaat atgctatgca aggaaaatta gttgaacaag acccaaatga tgaatcagtc
    12241 gaagttttac ttgaaaaaat acgagcagaa aaacaaaaac tctttgaaga aggcaagatt
    12301 aaaaagaaag atttggacat ttctattgtt tcccaag
//
LOCUS       DAVKMU010000051        11867 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKMU010000051 DAVKMU010000000
VERSION     DAVKMU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.051"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..674
                     /locus_tag="VW343_001733"
     CDS             <1..674
                     /locus_tag="VW343_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU9756251.1"
                     /translation="KRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFIK
                     ELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRYR
                     QNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYK
                     NLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYD
                     VGESL"
     gene            1358..1519
                     /locus_tag="VW343_001734"
     CDS             1358..1519
                     /locus_tag="VW343_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756252.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1611..3290
                     /locus_tag="VW343_001735"
     CDS             1611..3290
                     /locus_tag="VW343_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9756253.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            3558..4124
                     /locus_tag="VW343_001736"
     CDS             3558..4124
                     /locus_tag="VW343_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756254.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4446..4634
                     /locus_tag="VW343_001737"
     CDS             4446..4634
                     /locus_tag="VW343_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9756255.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4696..5454
                     /locus_tag="VW343_001738"
     CDS             4696..5454
                     /locus_tag="VW343_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9756256.1"
                     /translation="MVVYIRQSKLPSEVSINKYHAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            5512..6366
                     /locus_tag="VW343_001739"
     CDS             5512..6366
                     /locus_tag="VW343_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9756257.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6478..7362
                     /locus_tag="VW343_001740"
     CDS             6478..7362
                     /locus_tag="VW343_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9756258.1"
                     /translation="MKKVMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7501..7602
                     /locus_tag="VW343_001741"
     CDS             7501..7602
                     /locus_tag="VW343_001741"
                     /inference="COORDINATES: protein motif:HMM:NF014902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9756259.1"
                     /translation="MNTLGDKLMELSAIYHRPESEYAYLYKDKKLHI"
     gene            7666..9264
                     /locus_tag="VW343_001742"
     CDS             7666..9264
                     /locus_tag="VW343_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9756260.1"
                     /translation="MEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTE
                     GQNILYGDKGCVENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFAN
                     GNALLNPEGTLDWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSN
                     HKYNTTDYFEIDRYFGDKETFRELVDQAHHRGMKVMMDAVFNHIGSQSLQWKNVVKNG
                     EQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATY
                     WIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHA
                     VMNYPLSDSIKDYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWT
                     ANEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNF
                     MKRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEA
                     VALASNCQELENQLVISPDGFVIF"
     gene            9539..9994
                     /locus_tag="VW343_001743"
     CDS             9539..9994
                     /locus_tag="VW343_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756261.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            10439..10915
                     /locus_tag="VW343_001744"
     CDS             10439..10915
                     /locus_tag="VW343_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9756262.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            10915..11676
                     /locus_tag="VW343_001745"
     CDS             10915..11676
                     /locus_tag="VW343_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9756263.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSSVYDSGENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT         4294 a         1637 c         2451 g         3485 t
ORIGIN      
        1 acaaaaggat aaaaaggtta aggagaatat ctggaggaga tacctatacg ataatctacc
       61 taaaacttct actactaagc ctaaaagatg agggcaaact ctattatgac ggagtagaaa
      121 gtgattttat aaaagagtta gcactaacca tagatgaaac agacgatgat gtaatggtaa
      181 cagtaaatta tttaatcaat caaggcttat tagaagttgt aacagaaaat gatgaatact
      241 acctaactga aataccaaac ttaatcggat cagaaacagc ctgggcagag aaaaaaagaa
      301 gatacagaca aaataaacag aggacattgt ccttaatgag tccaacccat gtccgacaag
      361 agatagagat agagaaagat atagagatag ataaagagag agagggagag atagataaaa
      421 agtccacccc cattttttat ggagaattca aaaatgtaag gttaagcaaa gaagaatata
      481 aaaatctaaa agaaaaatta aactcacaca cagatataat gataaataaa ctatccagat
      541 acatggaaag cagtggtaag acctatcaaa accactatgt gacaatctta aaatggtatg
      601 aagaagataa agacaaacta agacagaaag gtttaaataa aaaaatgaat tatgatgtag
      661 gagaatcttt atagataaaa agaggtggaa aggcactatt aaagacttta agtttaaaaa
      721 ggtatcaaag tatgccagag ataaaaaaat ataaatatga cactttaaag gcgattagaa
      781 aattaaagaa atgctagaag acttttagat gacagttacc aagagggtct aattttcact
      841 gctacttatg cagtaggaat tggacctttt tatataagca aattctattt caaaattggt
      901 taccaagaaa gacttctttt cactgttaag tataagaggg taatttatag aattttgtta
      961 catcaagata aatattttct gatggtcaaa ttttaaaaag attaataact ctcgataaat
     1021 tttgaacctt aacaattgaa tagaccaaga caagacgtta attatttttg gagcgtaata
     1081 acctatccgt caagatcttt cggctgaata attcggcaaa aaaagttctg ctaagcaaaa
     1141 agaaagtgag cgataagtaa gagtttgaat atagggaggg ataccctatc cgctatgaag
     1201 aaaatgagga taatgatact tctaaggttg aagccggctc atccggaata gaggcggagg
     1261 tgagattcct atgttgctaa tccaaagcac ttgtcaatgt caaaaaactt aattaaacaa
     1321 ataattatta gatataaaac tttagaggag gtatatattg gacaatgact ggaatggatt
     1381 ggctgattta atagcaaatc tgattgcaaa atatgctggt gctttagatt tagataatct
     1441 acccgatcca acgccagcaa aaaatcaaga gatgaaaaat agttttgaca tggcaaaaac
     1501 acaaattgag acggactaat aaaagtgtta taatatactt gaaatgatag tccaaactta
     1561 aattaaaaaa attatatatg atatagaaaa attatattga ggttaatact atgtcaaaag
     1621 aaaaaataaa agtatacctc tatacacgag tatctacgtc aatacagata gaggggtatt
     1681 cgttagaggc acaaaaatca cgaatgaaag cttttgctat ttacaacgat tatgaaattg
     1741 ttggagagta tgaagatgcc gggaagtctg gcaagtctat tgaaggtaga atacagttca
     1801 atcgaatgat ggaagatata aaatctggaa aagatggagt atcttttgtt cttgtgttta
     1861 agttatcaag atttgcaaga aatgccgctg atgttctttc tacacttcag ataatgcagg
     1921 attatggagt taatcttatc tgcgttgaag atggcattga ttcatctaaa gatgctggga
     1981 aactaatgat ttctgtttta tcagctgtgg ctgaaataga aagagaaaac atccgtattc
     2041 aaacaatgga aggttgcatt caaaaagcaa gggaaggaaa atggaatggt ggctttgctc
     2101 cgtatggata taaacttgaa gacggcaagc tgtttataaa tgaggaagag gcagttgcaa
     2161 taagaacgat tttcgaccag tatgtaaata ctacgatagg agccaatggg atatctaaat
     2221 acttagagaa tcatggaatt agaaaaatcc caagacagaa tggtaagaat cctttgtttg
     2281 atgcaggtct tataagaaag atattaaaga atcctgtata taatgggaaa atagcatttg
     2341 gaagaagaac tttagaaaaa gttcatggta caagaaatga atataagcag gttgaacaag
     2401 atgaatatct aatatctgaa gggatacatg aagctatagt ttccgatgaa gtttggcaag
     2461 ctgctcaggt taagctaaaa tctcaagcaa agaaatatga gcatgtgaat aaaggaaaag
     2521 atacacgcac acacttgctt tctggaattg taaaatgccc gatatgtgga gtgggaatgt
     2581 ttgggaacaa gtgtatcaag aaaaagaaag atggcacaaa gtataaagat ttttattact
     2641 atggttgtaa acataggcag atgataagag gtcataagtg tacattcagt aagcaaatta
     2701 gagaagaatt gttagatgat gctgttgcag aggtgattgt caagatagta agtaatccga
     2761 aatttgcttc tatgatgcaa gaaaaaatca acatgaaggt ggatacctct gaaatagaaa
     2821 aagaaataga taattaccaa aaggaattga gaaagagtca ttctacaaag tttaagctaa
     2881 ttgaggaaat agataattta gatgttgaag ataagcacta taagagaagg aaacaggatt
     2941 tagatgatag actatatcgt atgtatgaca aaatagatga attagaatca tcactaattg
     3001 atgcgaaagc aaagaaacag actattgaag ctgaaaaact tacaggagat aacatatata
     3061 aggttctgat ctattttgat aagctctata aagtcatgaa tgatgtagag cgtaggcagt
     3121 taatttcagc tttgatttct gaaattcaag tttatgaaga aaaacaatct aacggacaat
     3181 ggctaaaatc cattactttt aagctaccaa ttatagaaga aaatctcaac atagatttgg
     3241 acaatgatga acaagttgaa tgtgtaagtt tattagagaa acgtagttaa tcctcaaaag
     3301 gttccccaaa ctttttgagt cccactgacg catcacgctg aggcggtatc actgcttgta
     3361 cgagttgaag catcagcgaa gtaaaagtag atgttcagcc aatgcgacga ggtgagaccg
     3421 aaggtcgata aggtgtgttc tgggtatagg actcgtaaag taagtaggaa atatgatgag
     3481 atgatacaga gagcctgaat tggtcaggtg acgtgcttat ttacattagc ctgataatga
     3541 aaatggagag attatcgatg gttatattgg aattgtacca aaatgattat tctaaagatt
     3601 tagtagcatt tgattccata gaagatggga aagcctttgt ggctcaaatc ccgggataca
     3661 ctctggaaac agaagatggt tttgaagtgg agtattttaa tcctaaaaac ataccggatt
     3721 atatggaaat tatttttaac ggaaatattg ttcctttatc taaatttatg ttcgatcctg
     3781 aggaaaatgt aaacattatt tggaaagaga tttcaaattt atccctcaaa aatgatagag
     3841 tgatcgaagg atattcaaaa attgatgcat atgtcgttaa caaccatgaa gttaaagcct
     3901 atgtcgaaac aagagaaacg aactatcgta aagcaaaaga cttcttagaa agccgcggat
     3961 atgaaatcga cagaagcttt ttcggaagtg aagacggtga agcgattctt tacagaaaaa
     4021 aagggatcga agtttggcat ttcttgtgtc acttagaccc aatgtttgta gagattgaag
     4081 atgtagaagg atatgttaaa gaagaagttg gagagattca atagtcaacc aaagttcctt
     4141 gtgagtatat acctttgcta gtaaaagagt caaaacgtta taacgaaggg ggggaagaaa
     4201 acccttgagt gtaagtcttt ttcttgttta gagggaaagt attagagtag ccgctcataa
     4261 tttatactag atttgataac tgatttgaga cagattttat ttctataaat attgtgaaaa
     4321 ttttttaata ccaaagattt aaagttactg tgttaaaaat cctctccact tttaaactaa
     4381 tgtattataa aagccagttg acacaagtgt tacacctgtg atagaattaa cttgaggtga
     4441 tgaaaatggc aaccgataaa aatagaataa tgattagctt ggatgataaa aatctagaga
     4501 aacttgaaaa tttagtagaa gatgctagag atagaagggg aatgcgacta acaaagtccc
     4561 aggttattga attactccta aataccgttg attatttcga tgatattatg ggggctattt
     4621 actcaaaaaa ataagagcaa aattgctatt attttttaca aattggtgta acactagtga
     4681 tataggagtg atgaaatggt tgtttatatt agacaaagta aattaccaag tgaggtttca
     4741 attaataaat accatgctca agtaggtgcc tacttacaag gagaagaagc tgttttatat
     4801 caatcttttt ctgaaataaa agagttaaca agtgaagata tagttgtaga ttatataatg
     4861 gagactagag cattactaaa aatgatgggc ttaaacgttc cggttcatga ctatcctatt
     4921 gagcttaaag agttttatgg tcgaaagatt tatgctggta ttttaggaga gattgtgaat
     4981 atacctgata attggggaaa atttattaag cctaaagctg gttcaaaagt ctttactgga
     5041 agagttgtta atggaaccca tgatttaata ggtattggtc tacctttcga ctatcctata
     5101 tggattagtg aggttgtaga attcatagct gaatggcgtt gttttgtgtt agatggtcgc
     5161 gtattagatg ttcgacccta tacaggtgat tatcatgcac aatttgatgc aagtgtaatt
     5221 gatgaagcga tatcatgttg gaaagatgcg ccaatagctt atggactaga tatcggtgtt
     5281 actcgcgatg gcagaacact tgttgttgaa gtaaatgatg gttatgcatt gggaaattat
     5341 ggcctatctc ctttaaaatc aatcaatttt catagagcta ggtggaaaga aatggtaaaa
     5401 ccctattttg aaaaaaatga aatttttaaa atccaacagg atgttgtttt ctaaaataaa
     5461 tatggtatat aaccaatata aaaagcatca aaatgaacaa ggaggaatct gatgcttgga
     5521 gcaattattg gagatattgt cggttctgtt tacgaatgga acaatatcaa aacaaaggac
     5581 tttcctttat ttcggaagga ctgctttttt acagatgaca cggttatgac ctgtgctgtt
     5641 gcagaagcaa ttatgaatgg tggacaaaaa gacgatttca ttgacgcgat gaagaaatat
     5701 ggcagaatgt atccgaatgc tgattacggt gctcggttta atgcatggct aaacagcgat
     5761 aaccgtgagc cttataatag ctttgggaat ggatcagcta tgcgtatttc tccatgtgct
     5821 tggatcatgg actgtggttt ttatgcgaaa actggtatgt ggccatcatc tagaggactt
     5881 acgagtcttt ctgcagaggt aactcataat catccagaag gtgtcaaagg cgctatggct
     5941 acagctgatg ctatctttct atgtcgtttt tactttggtg gttattgtag agagtacgag
     6001 caatcaatta acgacaatcc tacagagtgt aaaagacgaa ttaaggatta tatagaaaaa
     6061 gaatacgatt acaatctatc tcaaacttta gatgaaatcc gtcctaacta tcgttttaac
     6121 gaaacatgtc aggaaactgt ccctcaagcc attatcgcct ttcttgaaag tagagacttc
     6181 gaagatgcga taagaaatgc catctcactt ggtggcgaca gtgatactct cgcagcaatc
     6241 acttgtaaca tagcagaggc agcttacggt attcctgatt ggatcaagga taaggcctat
     6301 tcttacttgg atgaaccctt aaaggatgta gttaggcgat gggaaaatag aataaaagcg
     6361 tattaatctc cagtacgtca gatttctctt ttctgtattt tcattgtata ataggattac
     6421 atttacttgc atacgacgaa atgtaaacat ttctgtttaa aattaaggat aaaatcaatg
     6481 aaaaaagtaa tggtaattat caaccctact tctggtggcg agaaggcttt ggattacaaa
     6541 gaaaagctgg agaataaagc aaaagaatac tttgaatatg ttgaaaccaa aattaccgaa
     6601 aaagcgctgg atgcaacaca ttttgctgaa gaagcttctc gtgagcagta tgatgcagtg
     6661 gttgtgtttg gtggagatgg aactgtcaat gaagtcattt caggtattga tgagagagac
     6721 tacattccta agttagggat tatcccaggc ggtacgggta acctcattac aaaacttttg
     6781 gaaatcaatc aagacatcga tggcgcaatt gacgaactgg attttgattt aaccaataag
     6841 attgatatcg gtaaagcaaa tgacaactat tttggttata tctttagtat cggttctctg
     6901 cctgaggcga ttcacaatgt tgaaatagag gacaaaacaa aattcggtat tttaacctat
     6961 gctgtaaata ccatgaagtc tgtcatgaca gatcaggtct ttaacattaa ggttgagaca
     7021 gaaaatggaa attatgttgg tgaagctagc catgttttgg tccttttgac aaattacttc
     7081 gctgataaga aaatctttga agaaaacaag gacggctatg ccaacatttt gattctgaaa
     7141 gatgcctcta tattctccaa attatccgtc attcctgatt tattaaaagg ggatgttgtc
     7201 gcaaatgata atatcgagta tatcaaagcg cgtaatatta aaatctcttc agatagtgaa
     7261 ttggagtcag atgttgacgg agataaatca gataacctac ctgtagaaat caaagtccta
     7321 gctcagcgag tagaagtatt ttcaaaaccg aaagaggatt agtatataga gaaagccttt
     7381 tttaaggctt tttgtatact ttaaaagata gttcctttaa caacggacat tccttgcaaa
     7441 tagtttacaa aaatagtata ctggatttat tgagtttgaa aacgtttgcg taaaatttga
     7501 atgaatactt taggagacaa attgatggaa ttgagtgcta tttaccatag gcctgagtcg
     7561 gagtatgcct atctttataa ggataagaaa ctccatattt gaattcgaac taagaaaggg
     7621 gacattgaaa gcatcaactt gcactatggg gaccctttta tctttatgga ggagttttat
     7681 caggatacaa aagaaatggt caagataact tctggtacct tatttgacca ttggcaggtt
     7741 gaagtgtcag ttgactttgc acgtatccag tatctctttg agctcagaga tacagaaggt
     7801 caaaatattt tgtatggcga taaagggtgt gtggaaaatt ctctagaaaa tcttcatgca
     7861 attgggaatg gatttaagtt gccttatctt catgagattg atgcctgcaa ggttcctgac
     7921 tgggtttcaa atacggtatg gtatcagata tttcctgaaa gatttgccaa tggcaatgct
     7981 ctattaaacc cagaagggac tttagactgg gattcatctg tcacacctaa gagcgatgat
     8041 ttctttggtg gtgatttaca ggggattatt gatcatatgg attacttgca agacttgggt
     8101 attactggac tatatctttg tcccatcttt gaatctacaa gcaatcacaa gtacaatacg
     8161 acagattact ttgaaattga ccgttatttt ggagacaagg agacctttcg ggaactggtg
     8221 gatcaagcgc atcatcgtgg catgaaagtc atgatggatg cggtatttaa tcatattggt
     8281 tcgcaatctc ttcaatggaa aaatgtcgtc aaaaatggcg aacagtctgc ttataaggat
     8341 tggttccata ttcaacaatt cccagtgaca actgaaaagc tagttaataa gagagactta
     8401 ccctatcatg tttttggttt cgaggactat atgcctaagc taaatacagc caatccagag
     8461 gtcaagaatt atcttttaaa ggttgcgact tattggattg aagagtttaa tatcgatgct
     8521 tggcgtttgg atgtggctaa tgagattgac catcagttct ggaaggattt tcgtaaggca
     8581 gttttagcta aaaatcctga tctttatatc ctaggagaag tctggcatac atctcagcct
     8641 tggctaaatg gagatgagtt ccatgccgtc atgaattatc ctttatctga tagtatcaag
     8701 gactatttct tacgaggaat taagaagaca gaccagttca tcgatgaaat caatggagag
     8761 tctatgtatt acaagcagca gatttcagag gtcatgttta atctcttgga ttcacatgat
     8821 acagagcgaa tcctgtggac ggccaatgaa gatgttcaac tggttaaatc agccttagcc
     8881 tttctctttt tacaaaaagg aacaccgtgc atttattacg gaaccgagct agccttgact
     8941 ggaggaccag atccagattg tcgtcgttgt atgccttggg aacgtgtatc aagtgacaat
     9001 gatatgctga actttatgaa gaggctgatt aagattcgga aatacgcgtc agtaatcatt
     9061 tcgcatggca agtatagcct tcaagaaatc aaatctgatc tagtagctct ggaatggaaa
     9121 tacgaaggac ggatcctcaa agcaatattc aaccaatcaa cagaagatta tcttttagag
     9181 aaagaagcag tagcactagc aagcaattgc caagaattgg agaatcagct tgtcatctct
     9241 ccagatggat ttgtgatttt ctaaaaacta gttgatgaag attatggtac atttcatatc
     9301 ttatatagta taataaggct agttactaaa cttgtaaagg agaacttaaa tgaattgtag
     9361 aggacatgaa acaagacaaa gaattgttag agattttgaa gtttagccta aagcacatat
     9421 taagctgtta gcaaatcaac aaaaacatag tgatgcagga gcaactattg aagatgaata
     9481 ttatgtattt atcgctgaga gtaaaattga tggcaagaag gaagttattc agtgttgcat
     9541 gggtgcggca agggattttt tagaactaat taatcacaaa gggctacctc tttttaatcc
     9601 gcttgtaggt gattctcatg taaataatag acaagaatat gacaatacag ggagtggaaa
     9661 tttacaacct gaaaagtgga atgaaactgc aaagcagctt tataatgcta taatgtggtt
     9721 gattatttta tggaatgcta agccggatac acctttattt aattttaaag acgaagtaat
     9781 taagtataaa acatatgagc cttttgaaag cagtataaaa agagtaaata ctactataaa
     9841 gaatggtagt aaagggaaaa ctctgactga gatgattaat ggctacagag cggataacga
     9901 tattagagat gaaatttgta actttaatat tctgaaaaat aaaattcgtg atatgaaaga
     9961 ccaacaagga aatacaatgg aatcttactt ttagttattg ttgaattttg ggtattctat
    10021 aaaatatcct aattgagatt taaatagtag actatacaat atagttaaaa tatcagtaaa
    10081 aacaacactt tattgaggta ttggatacgc tttgctaata gcctaataat cacatgtgga
    10141 gtgttgctac aacgaaaaag gtgataatcc ttaatttcaa gctattttat aagcattttg
    10201 tctttgtaga taaaggcaat tttgacaata aaaatcctaa aaggtgaatc gttatagatg
    10261 tatttgtaga tattgtttgc gcatcgaaaa aattaataca agaataaata tttatagctc
    10321 tttaggtgac ttttatagaa gtaaagttta ggatagaaaa acaagaaata acgcaccatt
    10381 tttggtgcgt tatgcttttt tatgctataa tggatttata aaaataaagg agtttgctat
    10441 gattggaaag aacataaaat ccttacgtaa aacacatgac ttaacacaac ccgaatttgc
    10501 acgaattata ggaatttctc gaaatagtct gagtcgttat gaaaatggaa cgagttcagt
    10561 ctctaccgaa ttaatagaca tcatttgtca gaagtttaat gtatcttatg tcgatatcgt
    10621 aggagaagat aaaatgctca atcctgttga agattatgaa ttgactttga aaattgaaat
    10681 tgtgaaagaa agaggtgcta atctattatc tcgactctat cgttatcaag atagtcaggg
    10741 aattagcatt gatgatgaat ctaatccttg gattttaatg agtgatgatc tatctgactt
    10801 gattcagaca aatatctatt tagtagaaaa ttttgatgaa atagagagat atagcggcta
    10861 tttggatgga attgaacgta tgttagagat atctgaaaag cggatggtag cttaatggaa
    10921 atccaagatt atactgatag tgaattcaaa catgctttag caaggaatct tcgttcactg
    10981 acaagaggaa aaaagtccag taaacaacct atagcgattt tgcttggagg tcaaagtggt
    11041 gccggtaaga ctacaattca tcgtattaaa cagaaagaat ttcaaggaaa tattgttatc
    11101 atagatggtg atagttttcg ttctcagcat ccacactatt tagaactgca gcaagaatat
    11161 ggcaaagaca gtgtagaata taccaaagat tttgcaggaa aaatggtaga gtctttagta
    11221 acaaaattga gtagtttgag atacaatctt ttgatcgagg gaactttacg aacagttgat
    11281 gttccaaaga aaacagcaca actcttgaaa aataagggat atgaagtaca attggcctta
    11341 attgcgacaa agcctgaatt gtcgtatcta agtactctta tccgttatga agaactgtac
    11401 attatcaatc caaatcaagc acgcgcaact ccaaaagaac atcatgattt cattgtaaat
    11461 catctagttg ataatacacg acaattggaa gaactggcta tctttgaaag aattcaaatt
    11521 tatcaacgag atagaagttc tgtatatgat tcaggagaaa atacaacttc agcagcagat
    11581 gttcttcaag agttactgtt tggggagtgg agtcaggtag agaaggagat gttgcaggtg
    11641 ggggaaaaga gacttaatga attacttgaa aaataaacaa ttgatatttt taggagagta
    11701 gaaatgagag ggtttaataa caagataaag tctgtttatc aagaactaac aaattccaaa
    11761 gagaaattcg gtagctttca caagacttta attcatttgc atacacctgt ttcttatgat
    11821 tacaagctat tttctaattg gactgcaacg aaatatagaa aaattac
//
LOCUS       DAVKMU010000052        11357 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKMU010000052 DAVKMU010000000
VERSION     DAVKMU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.052"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(172..786)
                     /locus_tag="VW343_001746"
     CDS             complement(172..786)
                     /locus_tag="VW343_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9756264.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIQRL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1601..1870)
                     /gene="rpsO"
                     /locus_tag="VW343_001747"
     CDS             complement(1601..1870)
                     /gene="rpsO"
                     /locus_tag="VW343_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9756265.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2011..2640)
                     /locus_tag="VW343_001748"
     CDS             complement(2011..2640)
                     /locus_tag="VW343_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9756266.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADEFDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNGKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            3499..3822
                     /locus_tag="VW343_001749"
     CDS             3499..3822
                     /locus_tag="VW343_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9756267.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            3880..5823
                     /gene="thrS"
                     /locus_tag="VW343_001750"
     CDS             3880..5823
                     /gene="thrS"
                     /locus_tag="VW343_001750"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9756268.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5872..6846)
                     /locus_tag="VW343_001751"
     CDS             complement(5872..6846)
                     /locus_tag="VW343_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9756269.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6839..7516)
                     /locus_tag="VW343_001752"
     CDS             complement(6839..7516)
                     /locus_tag="VW343_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9756270.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7672..8745
                     /locus_tag="VW343_001753"
     CDS             7672..8745
                     /locus_tag="VW343_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9756271.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            complement(8836..9138)
                     /locus_tag="VW343_001754"
     CDS             complement(8836..9138)
                     /locus_tag="VW343_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756272.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9207..9644)
                     /locus_tag="VW343_001755"
     CDS             complement(9207..9644)
                     /locus_tag="VW343_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9756273.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9720..10562)
                     /locus_tag="VW343_001756"
     CDS             complement(9720..10562)
                     /locus_tag="VW343_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9756274.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10674..11324
                     /locus_tag="VW343_001757"
     CDS             10674..11324
                     /locus_tag="VW343_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9756275.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
BASE COUNT         3498 a         2540 c         2051 g         3268 t
ORIGIN      
        1 gtcctattct tatttcattt tactatattt ataagaatcc cctaatcaaa tcaaaagcca
       61 ctctacaata gagtaacttt aagattgtcc atttatactc aatgaaaaag ccctctgaaa
      121 atcagagagc tgatttgagc tcaggctaaa atcctagtga aacaaaaaaa tctacacgat
      181 cagaaaagtc tctatcgtgc cattttcata catgatgtat agtccaagta gaatgaatac
      241 taagggcaca atgattcgct cgtatttttc aattgtctcg aatattaacg gaatagagga
      301 taacacccta ctaatctcgc aaaagataat tatgccgatt acaaacacaa gcaaggccac
      361 gagggtctgt gaccaatcta acgaagcaaa ataaggtata tagataccta aattatctcc
      421 gccagacgca attgtcagca atgtaactgt ccaaaacagt tgatttgcct tgctttgttc
      481 taatctttga ataatttctt cctcttcttc ttccgcatct tctccaacaa ttgcaaagcg
      541 aatccctaaa tagataggga ttaaaccaag caatccaacc atccattctt caggcacgaa
      601 attaacgaca taagcagcaa ctaaactcgc ccctacaagt aagcctgtgc ctagatattg
      661 ccccgcataa atatgccatt tctgtttatt ctgtgatagc tgtgcaaata aaataattaa
      721 aataattaaa taatcgatac tggtggaaat ataaacacca atagcagata tgattgtctg
      781 tcccataaaa aaaacctcct gtattagtca gtaataaatc agcagatttt aggagagcac
      841 aaacacatta atttagctat cgctagtggg taatgtcgaa cgtaggaaag cattttactc
      901 ctcctcaaaa cgtagttaca aagtaatttc taactggaat tcagtgacta cttccatgta
      961 aagtataaca tactatactt tactttgtaa agagtgggct cttctagggg gcttgcatac
     1021 agctactcgg cttgaaaagc cgagtagctg cgcaccttta tggtgtaatt agctgatacc
     1081 atttgagatt tttgtagtct ccaaaattgt taccgataaa acctcaataa aaaagccctc
     1141 tgaagtcaga gagctgattt tagcccgggc taaaatccta gtgaaaaaga tgaaactcct
     1201 tgtgctcatc gaacactgtg tcatttccct attttcatac ggatttttga cacccttagc
     1261 atcatgattc ttgctagata aatcgtttat agactagggg acgaggtgaa gatcgtacga
     1321 aactttcagt gaaacaaaaa aatctccacg aagggagaaa agctagttta ttttacctta
     1381 cagtgctagg aaccgtaacc gaagattata cttgagtata gcgaggtaag gtgacaacgc
     1441 aacaaagcta cccaatttag agtagcttta ttatcaagat atgctcaatt gaacacggcc
     1501 taagcacttg gcaaaaaaga taaaatctcc tagaaactga agtttcttcg tcaattttcc
     1561 tatttttgct ttgtgctttt gacggccttt gtatcttgaa ttagcgacga agtcctagag
     1621 agttgatcaa ctcacggtaa cggttaacgt cgtttttacg caagtatgca agcaagttac
     1681 gacggcgacc gatttttttc atcaatccac ggtaagtagc gtggtctttt ttgtgttgtt
     1741 tgatgtgttc gttaaggtgg ttgatttccc aagtaaggac agcaacttga acctctactg
     1801 aacctgtatc accttcgtga cgtgcatatt gtgcaatgat ttcatttttt ttctcttttg
     1861 agattgccat gatgtttctc ctttttattt ggcttcatcc gagtgacagg ttggcatgcc
     1921 tgtaaccaag aagaagttat ttgtctttac gacaatccct attttaccaa gatttaaatt
     1981 ttctgtcaaa cattaatact ctcatagtta ttatttttca agtaaagaga tacgttcttt
     2041 tatatgatga cctttttcaa gttcatctac caatccaata gcataatctg cataactaat
     2101 ctggcttatt ccttttccat tagtagtaaa aatttctcct gctagaatgt agtttcctgt
     2161 tttttcacca tcgggaataa aatctacagc aggactgaca aaagtccagt tgagattatt
     2221 ttttgtacga agaagatcaa attcatccgc ttgagctttt gctagtgact taaattcttc
     2281 aggaaaatca gacgtatcta aaagtcgtgt cgtttttgtt tcatctatat aaagacttcc
     2341 tgctccccct acaactagaa aacgtgtctg tgttccagca agaagctgat taaatagttc
     2401 gattgatttg ctgtggagcg gtagcgtatc tggtgtataa gcaccaaacg ctgaaataac
     2461 agcatcaaat ccagtaagat catcttttgt caactcaaat aaatcttttt taataataga
     2521 ctcagcttga cttttgtttt cagaacgaac aatagccgtt acttcatgtc ctcgtttgac
     2581 tgcttcttca acaattgctt tccccgcttg tccatttgct gcaataactg ctagtttcat
     2641 tttttatacc tctattgttg taattatttt agttacaaca aatgtgacac tcttaataat
     2701 caatatcaat agtcttgctt aattattatc aaaatatttc taccaagaaa actaactatg
     2761 attctagtga aaagaaaaat cttctttgtc aacaaattta ctttcttgtt ttaaacatgc
     2821 tataataatc atagcaagag atctaagttg tctgtttttt taaaacgagg tgattatcat
     2881 gcgtagattc tattcccatc tcccctacta tctggtcata ttattctttt attggccact
     2941 ttatgagttg ttcttgctag ttgtttctga cccctttaca ctatataaac aatcttctct
     3001 tctttacacc tctggtaatc ttgattgtat cgttactcta tagctaccgt ttccgtttct
     3061 cactttgatg gttagttggt aacggactgc tcttttactt tactatcata acctttggtg
     3121 agtttatact aatttacttg ctaatctatg aaacagttgc tctggtgggc atggattctg
     3181 gtattagcat caagcatatt ctacaaaata tgaaaaacaa aaaactttca caaaatcctt
     3241 gaaaaatctc acaatcatgc tataataatc catagagaca agtcacttag tccctttcta
     3301 ctagagagtg cgtggttgct ggaaacgcat aggaagtcta aactgatact actcttgagt
     3361 tttttatgaa aacataaaac ggtggccacg ttagagccga tcagaggtgt ccctctcttt
     3421 tgaggtacat aaatgaaggt ggaaccacgt tgcgacgtcc tttcgaggat gtcgcatttt
     3481 tttattagga tactaattat ggagttgcaa gaattagtgg agcgcagttg ggcaatccga
     3541 caagcttatc acgaactgga agttaagcat catgatttca agtggacggt agaagaagac
     3601 ctcttggctt tatctaatga tattggaaat ttccaacgac tggtgatgac aaagcaagga
     3661 cgctactatg atgaaacacc ctacacactg gaacaaaaac tttcagaaaa tatctggtgg
     3721 ctattagaac tttctcaacg tttggatata gacattctga cggaaatgga aaacttcctc
     3781 tctgataaag aaaagcaatt gaacgttagg acttggaagt agtctgctga taaaaaatca
     3841 atgcttagaa actatgaaat aataaaaaag gagaacatca tgattaacat tactttccca
     3901 gatggcgctg ttcgtgaatt cgaatctggc gtaacaactt ttgaaattgc ccaatctatc
     3961 agcaattccc tagctaaaaa agccttggct ggtaaattca acggcaaact catcgacact
     4021 actcgcgcta tcactgaaga tggaagcatc gaaattgtga cacctgatca cgaagatgcc
     4081 cttccaatct tgcgtcactc agcagctcac ttgttcgccc aagcagctcg tcgtcttttc
     4141 ccagacattc acttgggagt tggtccagcc atcgaagatg gtttctacta cgatactgac
     4201 aacacagctg gtcaaatctc taacgaagac cttcctcgta tcgaagaaga aatgcaaaaa
     4261 atcgtcaaag aaaacttccc atctattcgt gaagaagtga ctaaagacga ggcacgtgaa
     4321 atcttcaaaa atgaccctta caagttggaa ttgattgaag aacactcaga agacgaaggc
     4381 ggtttgacta tctatcgtca gggtgaatat gtagacctct gccgtggacc tcacgttcca
     4441 tcaacaggtc gtatccaaat cttccacctt ctccatgtag ctggtgcgta ctggcgtgga
     4501 aacagcgaca acgctatgat gcaacgtatc tacggtacag cttggtttga caagaaagac
     4561 ttgaaaaact accttcaaat gcgtgaagaa gctaaggaac gtgaccaccg taaacttggt
     4621 aaagagcttg acctctttat gatttcacaa gaagtgggac aaggtttgcc attctggttg
     4681 ccaaatggtg cgactatccg tcgtgaattg gaacgctaca tcgtaaacaa agagttggct
     4741 tctggctacc aacacgtcta cactccacca cttgcttctg ttgagcttta caagacttct
     4801 ggtcactggg atcattacca agaagacatg ttcccaacca tggacatggg tgacggggaa
     4861 gaatttgtcc ttcgtccaat gaactgtccg caccacatcc aagttttcaa acaccatgtt
     4921 cactcttacc gtgaattgcc aatccgtatc gctgaaatcg gtatgatgca ccgttacgaa
     4981 aaatctggtg ccctcactgg ccttcaacgt gtacgtgaaa tgtcactcaa cgacggtcac
     5041 ctattcgtta ctccagaaca aatccaagaa gaattccaac gtgcccttca gttgattatc
     5101 gatgtttatg aagacttcaa cttgactgac taccgcttcc gcctctctct tcgtgaccct
     5161 caagatactc ataagtactt tgataacgat gagatgtggg aaaatgccca aaccatgctt
     5221 cgtgcagctc ttgatgaaat gggcgtggac tactttgaag ccgaaggtga agcagccttc
     5281 tacggaccaa aattggatat ccagattaaa actgcccttg gaaaagaaga aaccctttct
     5341 actatccaac ttgatttctt gttgccagaa cgcttcgacc tcaaatacat cggagctgat
     5401 ggcgaagatc accgtccagt catgatccac cgtggggtta tctcaactat ggaacgcttc
     5461 acagctatct tgattgagaa ctacaagggg gccttcccaa catggctggc accacaccaa
     5521 gtaaccctca tcccagtatc taacgaaaaa cacgtggact acgcttggga agtggccaag
     5581 aaactccgtg accgcggtgt ccgtgcagac gtagatgagc gcaatgaaaa aatgcagttc
     5641 aagattcgtg cttcacaaac cagcaagatt ccttaccaat taattgttgg agacaaagaa
     5701 atggaagacg aaacagtcaa cgttcgtcgc tacggccaaa aagaaacaca aactgtctca
     5761 gttgataatt ttgttcaagc tatcctagct gatatcgcca acaaatcacg cgttgagaaa
     5821 taagagtcta gcataaaagc ctccaatctg gaggcttttt ctcatctatt tttactcaag
     5881 gactaagttc acttgagcaa actgaatccg cactgtcgtt ccttttccga cctcagactc
     5941 gatacgaatc tggtgcccca gttcttcaga aattttctta gatagataaa ggccaagtcc
     6001 agaggactgc tgggtcaaac ggccattgta tcctgaaaag ccacgttcaa atactcggag
     6061 gacatcactg ttttttatcc cgattcccgt atctttgata caaagctctt ggtcatccat
     6121 ataaatctcc agaccacctt ccttggtgta cttgagactg tttgagatga tttgctcaat
     6181 aaccactagc agccactttt tatccgtcac gatttcttta tcaaggtcat gtagattgac
     6241 atttaagcct ttttgaataa agaaaagagc atatttacga attatttcct tgaccaagtc
     6301 ctcaatttga acctgcttta agaccaaatc atcatggaaa ctttctaaac gcaggtactg
     6361 taaaactagg ttggtatagg agtcgatttt gaaaatttcc tgttctagtt gctgcttcag
     6421 ttggcggtcg accacttctg caactaagag ttgactggct gcaatggggg tctttatctg
     6481 atggacccac aaggtatagt aatccagcaa atccgtcagt tttctttctg cttttgacct
     6541 ctgctgatag agttccatct cacgcgcttc taatttttct gctaaagcta tttccaaagg
     6601 agacttggct tccctctctc catagagaag ttcctggcga tagacctgcg tttccaccaa
     6661 tatgtcccaa gtgaaaaata atatggttac aaagcaacac aagaagaaaa agtagaggaa
     6721 gtaaattcct agactggcaa ataaaaactg aaagagtaag acaagaaatg ccaaagaaag
     6781 cagatagata aaaagacgac tacgggagcg cagataggct agaaaaaatt gtttccaatc
     6841 aagcatgctt caatccgtac cctattcctt tcttggtctc gataaatcct accaatccct
     6901 gctcctccaa ctttttacgc aaacgagcca cattgacaga gagggtatta tcatcaatga
     6961 aaaagtcact gttccaaagt tcccgcatca ggtcgtcacg tgctacgatg ttgcctgcat
     7021 gctcaaataa cacgcgtaaa atctggaatt cattcttggt caaattcaag acttgccctt
     7081 gataatgtaa atccatggat ttggtattga ggataacacc agcatattcc agcaaactct
     7141 catcacgccc aaactcatag gaacgacgca acaagccctg aaccttagct aaaagaacct
     7201 gctggtcaaa aggcttggtc acaaagtcat ccgcccccat attgattgcc atgacaatat
     7261 ccatagcctg gtctctcgaa gaaagaaaca tgataggtac cttggaaatc ttgcggattt
     7321 cctgacacca gtgataacca ttaaacaagg gcaaaccaat atccatgagg accagatgag
     7381 gttccgactg aacaaataga ctcaaaactt ccataaagtc ttctaccagg accacttcaa
     7441 atccccattc agagagcatt ttcccaatct gttgacgaat gacctgatca tcttctatta
     7501 ataaaatctt gtgcatgcgc ttctcctttt ccattattat aacagatttt tccatgctag
     7561 atggtctgaa actgaatttg aaatagcctg tttttagcca gtacaaacag gctatgctac
     7621 tagctaattt gagggaaatt tgctaagata aataaaaaga aaggagctct tatggccaat
     7681 atttttgact atctgaaaga tgtcgcatat gattcttatt acgaccttcc cttgaatgag
     7741 ttagacattc taaccttaat agaaatcacc tacctctcct ttgataatct ggtctccaca
     7801 cttcctcaac gtcttttaga tctagcacct caggttccaa gagagcccac catgcttact
     7861 agcaaaaatc gccttcaatt attagatgaa ttggctcaac acaagcgctt caaaaattgc
     7921 aaactctccc attttatcaa cgacatcgac cctgaactgc aaaagcaatt tgcggctatg
     7981 acttatcgtg tcagcctcga tacctatctg attgtctttc gtgggacaga tgacagtatc
     8041 attggctgga aggaagattt ccacctgacc tatatgaagg aaattcctgc tcaaaagcac
     8101 gcccttcgct atttaaagaa cttttttgcc catcatccta agcaaaaggt tattctggct
     8161 gggcattcca agggaggaaa tctcgctatc tatgctgcta gccaaattga gcaaagtttg
     8221 caaaatcaga tcacagcagt ttatacattt gatgcacctg gtctccatca agaattgaca
     8281 cagactgcgg gttatcaaag gataatggat agaagcaaga tattcattcc acaaggttcc
     8341 attatcggta tgatgctgga aattcctgct caccaaatca tcgttcagag tactgccctg
     8401 ggtggcatcg cccagcacga tacctttagt tggcagattg aggacaagca cttcgttcaa
     8461 ctggataaga ccaacagtga tagccggcaa gtagacacaa cctttaaaga atgggtggcc
     8521 acagtccctg acgaagaact tcagctctac ttcgacctct tctttggcac tattcttgat
     8581 gctggtatta gctctatcaa tgacttggct tccttaaagg cgcttgaata cattcatcat
     8641 ctctttgtcc aagctcaatc cctcactcca gaagaaagag aaaccttggg tcgccttacc
     8701 cagttattga ttgatactcg ttaccatgca tggaaaaata gataatactc ttgaaaatta
     8761 aatgtataca aaacaaaaga cctagaatac atactttcat gttcattcta agtcttttta
     8821 aatagaatct aatagtcaat aaaaatcaaa gagcattgag agataatggg gcttggaacg
     8881 tccctctcgt ttcaacaaaa tgaccccatt atagattaaa aagatgccac ttagaaaaag
     8941 caaaaaagga agtaagacaa aggcaaatac ataaaaagct aactgaacat tctcgtatcc
     9001 atttttataa aaaaggtagg atagataaaa ataacttgaa atgagggata ataaaaataa
     9061 tactggattc cacaaacttc tattatcctt ccaaaatgac actataaagg ctaatacaat
     9121 ttctataacg agatacattt cttactcctt taatagctac attttatcat aattatccaa
     9181 agaaaaaaag agggcattta tccctcttaa tccttcatct gactctctgc atcggccacg
     9241 actttttcta gactggtctg accaagttct gcctccatag tcaactgaat tctctccaat
     9301 ttttgatcca aaacatcatg aatatgagct cctacagggc aatttggatt cggattgtca
     9361 tggaaactga agagttgacc tgtcttacca agacattcga ccgcctgata aacatctaaa
     9421 agactaatat ccttaaggtc cttgacaatc tctgttccgc ccgttccacg cgctactgaa
     9481 atcagctctg ccttcttcaa ctgggacaag atctttctga taatgacagg attgacccca
     9541 acactagcag ccagaaaatc actggtcacc ttgctttcct tcccctcgag ggcaatgatt
     9601 atcagcatat gagttgcaat ggtaaatcta cttggaattt gcatcctctt ctccttttta
     9661 cgaggctacc ctgcctctac tcttcttttt ctattattat acccttttta gttgtaatgt
     9721 caatcgttac cacttttcaa ccagtcgtct aactcccgat cgcaaccctc tttctgagcc
     9781 aattctctca aaaattcctg atgatgagta tggtggatcc cattgaccag actttcatag
     9841 taaacctcaa aatagggaag tctcaggtct ttagccagct gcaattcagc tgccacatcg
     9901 taatctaccc gtcggaagtc catatctacc aggcccttgt catcaaactc caaaatcata
     9961 tactgggccc gcaagtcctt ccgtagctga gcgtccaaaa agaaaggttg gccaatcgaa
    10021 cccggattga caatcaattg cccaccagtc ccgtaacgaa gcaactgctg gtgaatgtgt
    10081 ccataaacag caatatcaca gggaggatga gtcaccaagc ggtcaaactc ctcttgtttg
    10141 ccagtatgaa tcaactctcg cccccagttc ttatcaggca gatgatggct aattcccact
    10201 gtcaaatccc caaactgacg atgaatttga agaggttgat tgtggagcac ttcaatttct
    10261 tctagggaaa tttcctctaa aacatactgg cactggcgca agagatagcg ttgactaggg
    10321 cgagtactat ccaattcctt acggacacca tgccaaagac tgtcttccca gtttcccaaa
    10381 actctagccg taatcggtag ttgatccaac aagtccaaaa tccttctacg ccctgtccct
    10441 ggcatgagaa tatctcccaa aagccagtat tcatccactc ctagctgccg agcatctgcc
    10501 aaaacagcct ccaaggcggt ggtatttcca tgaatatctg aaagaagagc tattttcgtc
    10561 atatccatct cctcgttttt tctcttgcaa taagtataac ataaaaagtc acagctagag
    10621 aaatctagct ttttttgata tactagataa agatattaga caagaggaaa cgaatgaccc
    10681 caaacaaaga agactatcta aaatgtattt atgaaattgg catagacctg cataagatta
    10741 ccaacaagga aattgcggct cgcatgcaag tctctccccc tgccgtaact gaaatgatca
    10801 aacgaatgaa aagtgaaaat ctcatcctaa aggacaagga atgtggctat ctactgactg
    10861 acctcggtct caaactggtc tctgagctct atcgtaagca ccgcttgatt gaagtttttc
    10921 tagttcatca tttagactat acaagtgacc agattcacga ggaagctgag gtcttggaac
    10981 acactgtctc tgacctgttc gtggaaagac tagataaact gctaggtttc cctaaaacct
    11041 gcccccacgg aggaactatt cctgccaagg gagaactcct cgttgaaatc aataacctcc
    11101 cactagctga tatcaaggaa gctggcgcct accgcctgac tcggatgcac gatagttttg
    11161 acattctcca ttatctggac aagcactcac ttcacatcgg tgaccagctc caagttaagc
    11221 agtttgatgg cttcagcaat accttcacta tcctcagtaa cgacgaggat ttacaagtga
    11281 atatggacat tgcaaaacaa ctctatgtcg agaaaatcaa ctaatttctc aagtccccta
    11341 ccaaccctga aagtttt
//
LOCUS       DAVKMU010000053        10991 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKMU010000053 DAVKMU010000000
VERSION     DAVKMU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.053"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            52..774
                     /locus_tag="VW343_001758"
     CDS             52..774
                     /locus_tag="VW343_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9756276.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            771..1619
                     /locus_tag="VW343_001759"
     CDS             771..1619
                     /locus_tag="VW343_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9756277.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1651..2580
                     /gene="psaA"
                     /locus_tag="VW343_001760"
     CDS             1651..2580
                     /gene="psaA"
                     /locus_tag="VW343_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9756278.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2703..3194
                     /gene="tpx"
                     /locus_tag="VW343_001761"
     CDS             2703..3194
                     /gene="tpx"
                     /locus_tag="VW343_001761"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9756279.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3290..5998)
                     /locus_tag="VW343_001762"
     CDS             complement(3290..5998)
                     /locus_tag="VW343_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9756280.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQCSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATDQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMSQSAVSSVVQNASAIRLFDSVASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(6000..6701)
                     /locus_tag="VW343_001763"
     CDS             complement(6000..6701)
                     /locus_tag="VW343_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756281.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDVLNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6951..9361)
                     /locus_tag="VW343_001764"
                     /pseudo
     CDS             complement(6951..9361)
                     /locus_tag="VW343_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
     gene            complement(9414..10106)
                     /locus_tag="VW343_001765"
     CDS             complement(9414..10106)
                     /locus_tag="VW343_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9756282.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10641..10976)
                     /locus_tag="VW343_001766"
     CDS             complement(10641..10976)
                     /locus_tag="VW343_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756283.1"
                     /translation="MGGILLLICPFVLLGTAVNTAATTLNGGATAGAFSGVALLLNAL
                     KIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGGILAIIGGSLFLA
                     TLKKFKSEE"
BASE COUNT         3201 a         2523 c         2066 g         3201 t
ORIGIN      
        1 gacttaattt tttttttaat gtatattaag agacaggagg aatacaagtt tatgatacgt
       61 atcgaaaacc tcagtgtctc ctacaaagaa acgttggcac ttaaggatat ttcactagtg
      121 ctccatggac caacaattac cggcatcatt ggtccaaacg gcgctgggaa atcaacacta
      181 ttaaaaggta tgttgggaat tatcccacat caaggtcagg catttctcga tgacaaggaa
      241 gttaaaaaat ccttacaccg aattgcctat gtcgaacaaa aaatcaatat cgactacaac
      301 tttcccatca aggtcaagga atgcgtctcg ttaggactat ttccctctat tcctctcttt
      361 cgaagtttaa aggctaaaca ttggaagaaa gtgcaagagg cccttgaaat cgtcggccta
      421 gctgactacg ctgaacgtca aattagtcaa ctgtctggag gtcaattcca gcgggtcttg
      481 attgccagat gtttggtgca ggaagccgac tatatcctct tggatgaacc ctttgctggg
      541 attgactctg tcagtgagga aatcatcatg aatacgctga gagatttgaa aaaagctggg
      601 aagacggttc tcatcgttca ccacgacctc agcaagattc cccactactt cgatcaagtc
      661 ttacttgtca atcgagaagt gattgccttt ggtccaacaa aagaaacttt taccgaaacc
      721 aatctaaaag aagcttacgg taatcaactc tttttcaatg gaggtgacct atgattgcag
      781 aatttatcga tggattgcaa aaattccatt tcctacaaaa tgccttgata acagctattg
      841 tcgtcgggat tgtagctgga gccgtgggat gtttcattat tctacgcggg atgtcactca
      901 tgggagatgc catttcacat gctgtcttac caggtgtagc cctctccttc atcttgggcc
      961 ttgacttctt tatcggagcc attgtctttg gattgctagc tgccatcatc attacctaca
     1021 tcaaaggaaa ctcgattatc aaaagcgata ccgccatcgg cattaccttt tcttctttct
     1081 tagccctcgg tatcatcttg attggtgtcg ctaaaagttc aactgacctt ttccatatcc
     1141 tttttggtaa tatcctggcc gtccaagata cggatatgtt tattactatg ggtgtggggg
     1201 cagccatcct cttgttaatc tggattttct tcaagcaact cttgataact tcctttgatg
     1261 aactcttggc taaagccatg ggaatgcctg tcaatttcta tcactacctt ctcatggtac
     1321 tcctgactct cgtgtctgtg acagccatgc aaagtgtcgg aactatcctg attgtagcca
     1381 tgctgattac cccagctgca actgcttatc tgtatgctaa tagtctgaaa agcatgattt
     1441 tcctttcctc aaccttcgga gctactgctt cagttttggg actctttatt ggctatagtt
     1501 ttaatgttgc ggcaggttct agtatcgtgc ttacagctgc tagtttcttt ctcattagct
     1561 tctttatcgc tcccaaacaa cgatatttga aactgaaaaa taaacatttg ttaaaataag
     1621 gggcaaagcc ctaataaatt ggaggatcta atgaaaaaat taggtacatt actcgttctc
     1681 tttctttctg caatcattct tgtagcatgt gctagcggaa aaaaagatac aacttctggt
     1741 caaaaactaa aagttgttgc tacaaactca atcatcgctg atattactaa aaatattgct
     1801 ggtgacaaaa ttgaccttca tagtatcgtt ccgattgggc aagacccaca cgaatacgaa
     1861 ccacttcctg aagacgttaa gaaaacttct gaggctgatt tgattttcta taacggtatc
     1921 aaccttgaaa caggtggcaa tgcttggttt acaaaattgg tagaaaatgc caagaaaact
     1981 gaaaacaaag actacttcgc agtcagcgac ggcgttgatg ttatctacct tgaaggtcaa
     2041 aatgaaaaag gaaaagaaga cccacacgct tggcttaacc ttgaaaacgg tattattttt
     2101 gctaaaaata tcgccaaaca attgagcgcc aaagacccta acaataaaga attctatgaa
     2161 aaaaatctca aagaatatac tgataagtta gacaaacttg ataaagaaag taaggataaa
     2221 tttaataaga tccctgctga aaagaaactc attgtaacca gcgaaggagc attcaaatac
     2281 ttctctaaag cctatggtgt cccaagtgcc tacatctggg aaatcaatac tgaagaagaa
     2341 ggaactcctg aacaaatcaa gaccttggtt gaaaaacttc gccaaacaaa agttccatca
     2401 ctctttgtag aatcaagtgt ggatgaccgt ccaatgaaaa ctgtttctca agacacaaac
     2461 atcccaatct acgcacaaat ctttactgac tctatcgcag aacaaggtaa agaaggcgac
     2521 agctactaca gcatgatgaa atacaacctt gacaagattg ctgaaggatt ggcaaaataa
     2581 gcctctgaaa aacgtcattc tcatgtgagc tggcgttttt tctatgccca catttccggt
     2641 caaatcattg gaaaattctg actgtttcag atacaatgga agaaaaaaga ttggagtatc
     2701 ctatggtaac ttttctcgga aatcctgtga gctttacagg taaacaacta caagtcggcg
     2761 acaaggcgct tgatttttct cttactacaa cagatctttc taaaaaatct ctggctgatt
     2821 ttgatggcaa gaaaaaagtc ttgagtgtcg ttccttctat cgatacaggc atctgctcaa
     2881 ctcaaacacg tcgttttaat gaagaattgg ctggactgga caacacggtc gtattgactg
     2941 tttcaatgga cctacctttt gctcaaaaac gttggtgcgg tgctgaaggc cttgacaatg
     3001 ccattatgct ttcagactac tttgaccatt ctttcgggcg cgattatgcc ctcttgatca
     3061 acgaatggca cctattagca cgcgcagtct ttgtcctcga tactgacaat acgattcgct
     3121 acgttgaata cgtggataat atcaattctg agccaaactt cgaagccgca attgcagctg
     3181 ctaaagccct atagaaaaaa tcctctgtca caaagagttc cctgctcttt gaaacggagg
     3241 atttttgaat attagtaaat agaagttcaa tcagctaaaa ataactacct tactctacag
     3301 atttcagggc ttctaacata tcaacctttc tcagataata attgacgaag aaaccaagca
     3361 aggtcaaaat gatgcttact gctgccactg ggattacata gacttcccag cctacctgcg
     3421 gataaaagag aatagtcgca ggcgaaatca tttgaatcaa aaattggtgt aaatagaaac
     3481 cagctaccag accaagtacg attcccacaa gggatagcac aatcgtctca cggtaaatgt
     3541 agagggtgac ttcattatta tgaaaaccaa gaaccttggt agtggagagt tcacggattc
     3601 tctcagctac gttgatattg gtcagattgt aaaggatgac aatagctaat agaaccgata
     3661 cgatgaccaa gatggtcatg gtctgattga gtgagctagc gacagagtcg aagagtcgaa
     3721 tggctgaagc attttggaca acgctggaca ccgcagattg actcataagc aagcccgcct
     3781 gactttcgat actagttgca ctggtatccc ttaatgagac cagataagtg ttggcttggg
     3841 gtagctgtcc gtaaagttgc tcatagctag cctgactcat ataaataaag tgaccaacgt
     3901 agttctcagt aatagcagcg acctttagtt ccttaccttc aatttctaaa gtctgcccaa
     3961 ccttgacacc tgccagctgg gcgagtttag ctgtaataac gatgccatct tttaatgtca
     4021 gctcctgctg atgatgttga agatggataa agggagtcaa atcttccttc tctatcatca
     4081 taagagtaat gttttgaaga ccagccttgc ctttgaaatc cttgtctagc gttttagaat
     4141 agattttctg gtaggctagt atctcctgcc ctttcaacac ttctgctagc tctaccttgt
     4201 cctgatcggt cgcactagga ttttcagaga caagcatctg atactgttgg atttgttgaa
     4261 actgtttaga cggaactcct gctacagaag attggattcc caaacctgca aagagcagag
     4321 ctacagaacc tgccacacca aagattgtca tcaacattct ctgcttataa cgaaagatgt
     4381 tgcgggctgt taccttatga gtaaaactga gacgacgcca gataaaaccg atacgctcca
     4441 ataagatttt agctcctttg acaggaggtt taggaagtag aagctgggct gcttcggcat
     4501 gaagttccct ccaagccacc agataggctg gtaacacact cgccaaccag ctcaagacaa
     4561 gagctagtaa gctataggtc caatagaact gaatctgggt ttctcccacc accatgcctt
     4621 ttgtaatgac acttgaaatt acactggcta gcaaataatg accaagtata ctacctagag
     4681 ccgttccgac agtcccagct actagtccat aaaggagaaa cttggcgata atatccttac
     4741 tacggtaacc caaggcctta aaaatccctg catgagttcg ctcttcgtct acaaagcgag
     4801 tcatggtcgt aaaggtcacc atggctgcta cggcataaag taccacagga aagatattgc
     4861 ccactgctcg aatactggat gaagcattgc tatacataag atagccctga ccacctggca
     4921 tggtctgacg attatagacc tgataccttg gctccgccaa atcgtccaag acttgctgat
     4981 gtttttctaa tttttccttt tcttgggcta gattttgttc agcttgcttt agtttgtctt
     5041 cttccttgcc caattcctgc ttggcttggg taagttgagc actagcctgc tctctctgaa
     5101 cttgaggaaa caaggctagt tgactttctt gagcctgtat acgagcttga gcagctgcta
     5161 aacgacgctt gccttcctgc aaattagtct gagccttgtc aagggtctct tgccccttgt
     5221 ctagagactc ttgtccttct tttttcaaaa gttgcagacg taccttgcca ttatctgata
     5281 aagtttgttc aaggtcttcc tgatgttgct tggatttttc ttcataagct gatgaaaagg
     5341 catttaaacc tgctaaatct tgatatttca aacgagctat attgtagact ttctgatcaa
     5401 actgactagg taaaatcacc ccataggctg tcagagtccc acttccactt cctgcgtagc
     5461 ccatatctcg ctgggagagg atttcagccg aatccacaaa accagtaatg gtataagtat
     5521 ggtcttttaa agaggaatga ccctcttctt tttctttaaa actaatctcc tgtcccacgc
     5581 tgcattggcc ttgcaaatga gtggccaaag cgatttcctt gtctgactga ggaagtcgtc
     5641 cctttcttag ctgaaaggtt gaaattcgct ctggtttgga gtacagccga atggcatcct
     5701 gcccattatc catagtcaca tctgtcaaat agccaaactc gacctctgcg ccctccgtct
     5761 gttttagttc ttcttggtct gcttgatcca agccatagtt agacatgact gccaaatcca
     5821 aggtttgagc agttgttaaa taagcattag ctgtcgcctc catgttggga ctggttactt
     5881 tgaggcctac taaggctaga gatcccaaca tcatcaggat caagatggat aaaaaacgcc
     5941 ccttggagcc tgtgaaggac tgaattaagt ccttccaata agtttttcgc ttgatcatac
     6001 tagtactcca aactgtcaat atcctgagga tgctggttga gcaccacatc cttgacactg
     6061 gcatcgtgca tatgaatcac gcgatcagca atgggcgcca aagctccatt atgagtcacg
     6121 atgatcaccg tcgctccctt ttgacgagac atatcttgga gaattttcaa aacctgcttg
     6181 cccgtctgat aatccaaggc tccagtaggt tcatcacaaa gaagaatttt aggatttttg
     6241 gctaccgcgc gtgcaatgga gactcgctgt tgctcccctc cagaaagctg ggctggaaag
     6301 ttatttagac gatgagccag acctacatct gtcaagacct gatcaggatt caagacatct
     6361 gtcacaattt cagaagccag ttccacattt tccttagctg tcagattaga aactagatta
     6421 taaaactgaa aaacaaaccc cacatcattt ctacggtaat tggtgcgctg gtgggaacta
     6481 taatccgcaa tattaacacc atcaatccag atttcccctt catcattggt atccattccc
     6541 ccaagaaggt taagcactgt tgacttgcct gcacctgaag caccaaggat aataaccagc
     6601 tccccctttt caatctcaaa attcacatca cgattggcca caatctccgt gtccccaacc
     6661 tgataacgct tgtaacagtg tttcatctca atataagcca tcagacccac cctctatcaa
     6721 acaaattata ctcctcgaaa atctctcttc aaaccacgtc agcttccatc tgcaacctca
     6781 aaacactgtt ttgagcaacc tgcggctagc ttccttgttt gtgctctttg attttcattg
     6841 agtattactt cctattatca ttataacgct ttttcaacta gttgaaaact gcttatattc
     6901 ttaaatcctt attaaaaggc agtttcaaat tactgcctta taacatcttc ttaaaaataa
     6961 aattgaacaa gatcaccttc tgctgttgcc accgaaatac aatctttccc catgcatttc
     7021 gcttttattt tttcttgatt cattttaatc acactcttct caatatctgg ataagaaact
     7081 cgcaaatctc ctccactcct tgataaaatg gtcaactgta agagtttttt atcttcccag
     7141 ctcatgctca cttcaaaatg tccacgtgcc attaagcctg aaacagaacc tgttgaccaa
     7201 gcatcaggta gggcagctag aggtaccaga taagctgcat gagactggag taacatttct
     7261 gccatgccac tagtagcacc aaaattacca tctatctgaa aaggaggatg gctacaccaa
     7321 agatttggca aggtggatgt ctttaactgc tctgccaata atttatgggc tcgattgcca
     7381 tctcccaaac gcgcccagag attgatctta ttagccttgg accagcctgt gccgccatct
     7441 ccacgatcat tgaggctagc acgcgccgct tcaatatact cttgtccctt gtagctaaag
     7501 agattgccag gatagagtcc cactagatgg gaagcgtgcc gatgctgggc ctccactttc
     7561 tcattttgaa aatactgctc ttcctcctca taccactccc tgattcgacc agattgagtg
     7621 atttgcaaag gatttagtaa atcagacttc tccttaacct cagtcaacaa gtcctcatcc
     7681 agtcccaatt cctgagcagc ctgaataaaa tcatgaaata actgccaaat tagagattgg
     7741 tcataagtat tgccaatcga aatcggccca tgttctgggg aataagacgg agaagacacc
     7801 caacgctgcg cctgctgatc cttatgtaaa aaggcattcc aaaaacgaac cgtttccctc
     7861 aacatgggat aaattttctc tctgagatag tcttggtccc tataaaatga ataggcttca
     7921 taaacggttt gcatcatcca cgcattggca gctggtgacc aaccccaata gtaatcccaa
     7981 ccaggtgccg tccaaccaaa gggagtcgct tgagtatgaa ccaaccaacc attttcctca
     8041 cctttttgag agacgattcc tgcatacttt acagccgcta gacgaccata gacacgcaaa
     8101 tcatctacat agttgatgac cggaaagacc gcctctagga gattggtaac ataggctggc
     8161 caataattca tctgcagatt gacatttaag tgatagtccg aattccaagg aggattgtcg
     8221 accgcattcc agactccctg taggttagct ggtagagcat ctgggcagtc tctggacgaa
     8281 ctaatcaata aataccgtcc atactggaag aacagctcct ccaaagcctg cccttcttgt
     8341 ggcttataat tttttaacaa atcatctgta gtggatgcgt caacatcagc ttccaaatcc
     8401 aattgaacac gctggaataa ggcttggtag tcctcgatat gccttgattt caattgggta
     8461 tagccctttt ctttagctgt gtccaccaag tctctcacct gttgctctaa atctagtttc
     8521 ttgcgataat tgctagcagg attttgggca aaatccgtct tagcggccaa gaagagattg
     8581 gcataactgg ctcctgatat ctgaacccta tatgaccaaa ctctaatatc tccatccgtt
     8641 tcccaagcta gataactagc aaaccgcaga tcattatcct taactcttcc cttcatcaag
     8701 atatgagaat cagtaatatc caacttacac tccttgtaat cagacttttc ctgttcatac
     8761 tttccattag aagccaaatc acaggtcaag gatagttcta tagtaaaatc tagagtttcc
     8821 aacccttcct tagtaaaaca ttgaaccaag agatcatctg gaaaactcgc aaaagcttca
     8881 cgttcaaatc gcgttccctt atagacataa gaagtcgtcg caagtgcctt actaatattc
     8941 agctgtctct gatagtccgt cacctgagac aaagtcgtac cttgctggct gaactcaatg
     9001 tgaatatccc caaaagacag ataggtccca tattgactcg tttttggccc aattaggtgc
     9061 tgctcagcca gttccttagc cagattgtaa tctctcttct ccaaagcctg ccgaatctca
     9121 gctaaaaaac atactgatcc tgaagatttc caccctgata atctgaacta tcaggaagtg
     9181 gacctccaga ccagagactt ttttcattaa attgaatccg ttcagcccct ataaggccaa
     9241 atacttttgc tcctaaagaa ccattgccta tcggtaaagc ttcttcttcc caacccatgt
     9301 aagtggttga agctggttgc ttgtaggcca gtacataatc ttgtttttta ttccttatca
     9361 tacttccata ctaacataaa aagcctagaa atcaattcta gacttacaat tttttatttt
     9421 ccaaggtagt attcagaaac gacgttcaat ttttcgtcga attcgaatac caatggtggg
     9481 aagttaggga tttccacgtc catgatctcg tcatctgaca aacctttgat gtgttttaca
     9541 agggcacgga ttgagttacc gtgagctcct acgaatacgt ttttaccatc tttaagagct
     9601 ggagcgattt tatcttccca gaatggaaga gcacgttcca aagtcacttt caagttttca
     9661 gcatctggga taactgagtc gtcaagtgaa gcgtaacgac ggtctgtgtg agctgagtgc
     9721 tcatcatcac ggtccatgtt tggaggcaat acatcgtatg aacgacgcca gatgtgaact
     9781 tgctcatcac caaattgttc agcagcttca gctttatttt taccagtcaa accaccgtag
     9841 tgacgttcgt tcaagcgcca tgatttttca actggaaccc acaattggtc agaagcttca
     9901 agagccaagt tagttgtttt gatagcacgt ttcaatactg aagtgtaagc ttggtcaaat
     9961 ttgataccag cttctttgat caatttacca gcgtcaatcg cttgttgtgt acctttttca
    10021 gacaaatcaa catcagccca accagtgaaa aggttagctt tgttccattc agactcaccg
    10081 tggcgagcaa aaaccaattt taccattaga tggattctcc tttaaatttt ccgaggtttc
    10141 ccctcgttta tctattctat tttacacaat ttttcacaaa aaagctagtc aaaaccaaaa
    10201 aggaaaggac tgtttgacaa tgtttggcaa tcacattgtt tttggttgtt tctattctat
    10261 cttcattatt taaccttttt ttctgcgcat cgaataattt ttgagttgac tcttttagtt
    10321 tagtaaacga gtcattattt tccatagctg tgcctttgaa accttcctca gttttagtaa
    10381 aataatcatc aaaaatattt ttataaagat ctatataatc tgcataagtg cgtattgatt
    10441 caatcgcatc gtctgttaca accggtgtat cgaaagtgat tgtttctttt gaactattgg
    10501 atgttgctac tgtcttctca tcattacttg ttgaagtatt ctttgaactt tatgttccac
    10561 atgcaactag aagagaaaga gataatagag ctactcctat accgataaac ttttttgttc
    10621 ttttcatgct aaaatacctt ttattcttct gatttgaatt tcttcaatgt tgcaaggaat
    10681 agagatcctc cgataatagc aagaatcccc ccaacccatc ctaagaatgg aatgagacta
    10741 acttcaccag aaacaatcat tagtacagac ggagctgcac ctacacgctt atctccttta
    10801 tagtaaacaa tagcaatgat accaagaaca agatttgcaa tcttcaaggc attcaagagt
    10861 aaggcaacac ctgaaaaagc ccctgcagta gctcctccat taagagttgt agcagctgtg
    10921 tttaccgctg ttcccaacaa gacaaaagga caaataagaa gtaaaattcc tcccacaaga
    10981 ccgacgatac c
//
LOCUS       DAVKMU010000054        10437 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKMU010000054 DAVKMU010000000
VERSION     DAVKMU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.054"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            180..2108
                     /gene="glgB"
                     /locus_tag="VW343_001767"
     CDS             180..2108
                     /gene="glgB"
                     /locus_tag="VW343_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9756284.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRKDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATNITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            2098..3240
                     /locus_tag="VW343_001768"
     CDS             2098..3240
                     /locus_tag="VW343_001768"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9756285.1"
                     /translation="MKNEMLALILAGGQGTRLAKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGIDSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLELGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3230..4369
                     /gene="glgD"
                     /locus_tag="VW343_001769"
     CDS             3230..4369
                     /gene="glgD"
                     /locus_tag="VW343_001769"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9756286.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            4366..5799
                     /gene="glgA"
                     /locus_tag="VW343_001770"
     CDS             4366..5799
                     /gene="glgA"
                     /locus_tag="VW343_001770"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9756287.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5858..6229
                     /locus_tag="VW343_001771"
     CDS             5858..6229
                     /locus_tag="VW343_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9756288.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVPGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            6269..6502
                     /locus_tag="VW343_001772"
     CDS             6269..6502
                     /locus_tag="VW343_001772"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU9756289.1"
                     /translation="MISPQVKKETLEKWRKKLKLSKERTVAIGDGVNNLLMLKSAELG
                     IAFCSKEMLKKEIPHHVDKRDFLEVLPLIDCLE"
     gene            6511..7627
                     /locus_tag="VW343_001773"
                     /pseudo
     CDS             6511..7627
                     /locus_tag="VW343_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(7624..8070)
                     /locus_tag="VW343_001774"
     CDS             complement(7624..8070)
                     /locus_tag="VW343_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9756290.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            8234..9538
                     /gene="eno"
                     /locus_tag="VW343_001775"
     CDS             8234..9538
                     /gene="eno"
                     /locus_tag="VW343_001775"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9756291.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFLGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            complement(9711..10437)
                     /locus_tag="VW343_001776"
                     /pseudo
     CDS             complement(9711..10437)
                     /locus_tag="VW343_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3133 a         1851 c         2313 g         3140 t
ORIGIN      
        1 tagaaaaaga aaggtgtttt cgcaattttt tgaaaaaata ttgatttaca attgaacgag
       61 taagtgaact tgttgtagat tttcaaaata attaaagaag tatagtaatt tacttgaatc
      121 ttctctaaaa aggtagtata ataaaaacat agaaaacgct tacaggagaa agggagtata
      181 tggataatag agaagcatta aaaaccttta tgacgggtga aaatttttat ctccaacatt
      241 atctaggagc acatagggaa gaactaaatg gagagtatgg ctataccttc cgtgtttggg
      301 cacctaatgc tcaggctgtt cacttggttg gtgattttac caattggatt gaaaatcaga
      361 ttccaatggt aagaaaggac tttggggtct gggaagtctt taccaatatg gctcaagaag
      421 ggcatattta caaatatcat gtcacacgtc aaaatggtca tcaactgatg aagattgacc
      481 cttttgctgt caggtatgag gctcgtccag gaacaggggc aatcgtaaca gagcttcctg
      541 ataagaaatg gagggatgga ctttggctgg cacgaagaaa acgttggggc tttgctgaac
      601 gtcctgtcaa tatttacgag gttcatgctg gctcttggaa gagaaatcct gatggcagtc
      661 cttatagttt tgcccaattg aaagatgaac tcattcctta cctagttgag atgaactata
      721 ctcatattga gtttatgccc ttgatgtccc acccactagg tttgagttgg gggtaccagc
      781 ttatgggtta cttcgcttta gagcatgcct atggtagacc agaggagttt caagattttg
      841 tcgaggagtg tcatacccat aatattgggg ttattgtgga ctgggtacca ggtcacttta
      901 ccatcaatga tgatgcctta gcctattatg atgggacacc gacttttgaa taccaagacc
      961 ataataaggc tcataaccat ggttggggtg cccttaattt tgaccttgga aaaaatgaag
     1021 tccagtcctt cttaatttct tgcattaagc attggattga tgtctatcat ttggatggta
     1081 ttcgtgtgga tgctgttagc aacatgctct atttggacta tgatgatgct ccatggacac
     1141 ctaataaaga tggcggaaat ctcaactatg aaggttatta tttccttcag cgcttgaatg
     1201 aggttattaa gttagaatat ccagatgtga tgatgattgc agaagaaagt tcgtctgcga
     1261 ccaatattac gggaatgaaa gagattggtg gtctaggatt tgactacaaa tggaacatgg
     1321 gctggatgaa tgatatcctc cgtttctacg aagaagatcc gatctatcgt aagtatgact
     1381 ttaacctggt gactttcagc tttatgtatg ttttcaagga gaattatctc ttgccattct
     1441 cgcacgatga agtggttcat ggcaagaaga gtatgatgca taagatgtgg ggagatcgtt
     1501 acaatcaatt cgcaggcttg cgcaatctct atacgtacca aatttgtcac cctggtaaga
     1561 aattgctctt catgggtagc gaatacggtc aattcctaga atggaaatct gaagaacagt
     1621 tggaatggtc taacctagaa gacccaatga atgctaagat gaagtatttc acttctcagc
     1681 taaaccagtt ttacaaagat catcgctgtc tgtgggaaat tgataccagc tatgatggta
     1741 ttgaaatcat tgatgcggat aatcgagacc agagtgttct ttcctttatt cgtaagggta
     1801 aaaagggaga aatgttagtc tgtatcttta atatggtacc tgttgagcgg aaagatttta
     1861 caatcggact acccgttgca ggaatttacg aagaagtatg gaatactgag ttggaagagt
     1921 ggggaggcgt ttggaaagaa cataatcaaa ctgttcaaac gcaagaagga ctatggaaag
     1981 attatgagca gaccttaacc tttaccctac cggctatggg agcaagtgta tggaaaatca
     2041 aacgtcgctt gaaatctact aaaaccgtca caaataaaaa ccaaaaagga gtagaaaatg
     2101 aaaaatgaaa tgttagcttt gattcttgct ggtgggcaag gaactcgtct cgctaaactc
     2161 actcaaagca tcgcaaaacc agctgtgcaa tttggtgggc gctaccgtat cattgacttt
     2221 gccctatcaa actgtgccaa ctcagggatt cacaatgttg gggtcgttac acagtatcaa
     2281 ccacttgctc tcaacaacca tattgggaat ggttcaagct ggggactaga cggtattgat
     2341 tcaggtgtct ctattcttca accttattct gcaagtgaag gaaatcgttg gtttgagggg
     2401 actagtcacg ctatttacca aaatatcgac tatatcgaca gtgtcaatcc tgagtatgtc
     2461 ttgattttgt ctggggatca catctacaaa atggactatg atgatatgct ccagtctcat
     2521 aaggataata atgccagctt gacagtagca gttttagacg tccctcttaa agaagcaagc
     2581 cgttttggta tcatgaacac agatgctaac aatcgtattg ttgaatttga agaaaaacca
     2641 gctcaaccta aatctacaaa agcttctatg ggaatctaca tttttgattg gcaacgcctt
     2701 cgtaatatgt tagtcgctgc tgaaaagagc aaggttggca tgtcagactt tggtaaaaat
     2761 gtcattccaa attaccttga gttaggtgaa agtgtttatg cttacgaatt tagtggttat
     2821 tggaaagatg ttggtactat tgagtcactt tgggaagcga acatggagta tatttctcca
     2881 gaaaatgcct tggatagtcg taaccgtcaa tggaagattt actcaagaaa cttgatttca
     2941 ccaccaaact tcctcggggc aaatgctcat gtggaagact cattagttgt agacggatgt
     3001 ttcgttgatg gaactgttaa acattctatc ctttcaacag gcgcgcaagt tcgcgaagga
     3061 gcggaagtcc ttgattcagt tatcatgagt ggagctatca ttggtcaagg agctaagatt
     3121 aaacgtgcca ttattggtga aggtgcgatt atttctgacg gtgtcgaaat tgatggaaca
     3181 gatgaagtac aagttgtagg atataatgaa gtagtggggg tagcaacaga tgaagattga
     3241 taaatattct gccattttag gaaatacagt tggttttcac aatatgtcga cattgacgga
     3301 ccaccgtcca gtagcaagtt tgccatttgg tgggaaatat cgtttgattg atttcccact
     3361 ttcaagcctt gctaatgcag gtgttcgtag tgtctttggt attttccagc aggataatat
     3421 cagctcagta tttgaccata ttcgttcagg acgcgagtgg ggcttatcaa cccttcttag
     3481 ccattactat ctaggaattt acaatacccg tgtagaaagt agtacagttg gaaaagaata
     3541 ctaccaacag cttcttactt atttgaaacg ttctggctca aaccaaacgg ttgccctcaa
     3601 ctgcgatgtt cttattaaca ttgatttgaa ccaagttttc cacctacata gtacaacaaa
     3661 agagcctatc actgtagttt ataaaaaact agctaagaaa gatatttcag aagtaaatgc
     3721 aatcttggat gtagatgaaa cagaccatgt tctttctcat aaactctttg atagcaagtc
     3781 aacagctgaa acgttcaata tgtctacaga tatctttgtc gttgatacac cttggttgat
     3841 tgaacacttg gaagaagaag ctaaaaaaga acatccagag aaattgcgct atgttttacg
     3901 ggatttggct gtaaaagagg gagctttcgc ctacgagtac acgggctatc tagccaatat
     3961 tcactctgtt aaatcttatt atcaagcgaa tatcgatatg cttgaatcac aaaaattcta
     4021 ttctcttttc tcaccaaacc aaaagattta tacaaaggtc aaaaacgaag agccaactta
     4081 ctatgctaat acatctaagg taagcacttc tcagtttgcc tctggtagta ttattgaagg
     4141 tcaagtagct aattctgttc tatcacgtaa tattcatgtc cataaggata gcttggttaa
     4201 agatagcctg ctcttccctc gtgttgttat tggagaaggg gctcaggtcg aatatgctat
     4261 cttggacaaa ggggttgaag ttgagcctgg tgttgtgatt cgaggaactg cagaacatcc
     4321 agttgtggtt aagaaaggtg ctaaagtaac agaggatatt cattcatgaa aattttattt
     4381 gtagcagcag agggtgcacc cttttcaaaa acaggtggtt tgggagacgt cattggcgct
     4441 cttccaaaat cactggtaaa agctgggcac gaagttgcag tgattttacc ctactatgat
     4501 atggtagagg ctaaatttgg aaatcagatt gaagatgtgc ttcattttga ggtgagcgtt
     4561 ggttggcgca gacagtattg tggaattaag aaaacagtat taaatggtgt aaccttctac
     4621 tttattgaca atcaatatta tttcttccgt ggtcatgttt acggtgattt tgatgacgga
     4681 gaacgctttg cctttttcca actggctgcc attgaggcta tggaaaggat tgactttatt
     4741 cctgatcttc tccatgttca tgactaccat acagctatga ttcctttctt gttgaaggaa
     4801 aaataccgtt ggattcaagc ctatgaggac attgaaacag ttttaaccat tcataattta
     4861 gaattccaag gacaattttc agaaggaatg ttgggtgatt tgtttggagt tggctttgaa
     4921 cgttacgctg atggcaccct tcgatggaac aactgtctga actggatgaa ggcaggtatt
     4981 ctctatgcga accgtgtttc aaccgtttca cctagctatg ctcatgaaat tatgactagt
     5041 cagtttggat gtaatttgga tcagattctt aaaatggagt ctggtaaagt atctggtatc
     5101 gtgaatggga ttgatgctga tctttataat cctcagacgg atgctctttt agactatcat
     5161 ttcaatcagg aagatttgtc tgggaaagcc aaaaataagg caaaattgca agaaagagtt
     5221 ggcttgcctg ttagagcaga cgttccactg gtgggaattg tttctcgttt gacacgtcaa
     5281 aaaggttttg atgtggtggt cgaaagtctt caccatatct tgcaagaaga tgttcagatt
     5341 gttcttttgg gaactggcga tccagccttt gaaggagctt tctcatggtt tgctcagatt
     5401 tacccagaca agctatcaac aaatatcact tttgatgtca aacttgctca ggaaatctac
     5461 gctgcttgtg acctcttcct catgccaagt cgttttgaac cgtgtggctt gtctcaaatg
     5521 atggctatgc gttatggaac cttgccattg gtccatgaag ttggaggctt gcgagataca
     5581 gttcgcgctt tcaatccaat cgaaggaagc ggtactggct ttagctttga caatctatct
     5641 ccttattggt taaattggac tttccaaaca gcattggact tgtatagaaa ccatccagac
     5701 atttggagaa acctacaaaa acaagctatg gagagtgact tctcatggga tacagcctgc
     5761 aagtcatacc ttgacttgta ccatagttta gttaattaat agataaaatc gtatgatgac
     5821 ttcatacgat ttttaggctg tttagagagg agaactgatg tctcaagtaa aaggcttgtg
     5881 tgttttggat gtagatggaa ccttaatcct agaagaagtg attgatcttt tagggagaga
     5941 ggcaggtcat gaggcggaaa tttcgcagat tacaagtcga gcaatgcgag gagagttggt
     6001 ctttgaaagc agtttaagaa aaagagtgtc cttgttggaa ggtcttccta ttttggtctt
     6061 tgataacgtc ttcaactcaa ttcatctatc gctaaatgtc ccagagttca tctctattct
     6121 ccaaaagaat ggcatcctag ttggtctggt gcctggtgga tttacaccaa tagttggaga
     6181 gattagcaaa atcccttggt attgcctatt tcactgccaa ccagcttgaa gtcaaagaag
     6241 gtttttaaca ggaaaattag ttggacaaat tataagtccc caggtcaaaa aagaaactct
     6301 ggaaaaatgg agaaagaaac taaaactttc taaagaaaga acggtggcaa tcggtgatgg
     6361 ggtcaataat ctattaatgt tgaagtcggc ggagttagga atcgcctttt gttccaaaga
     6421 aatgctcaaa aaagaaatac cacatcatgt tgacaagagg gattttttag aagttcttcc
     6481 tttgattgac tgtttagaat gagggaaaat atgaagattg taattgcacc ggattcgttt
     6541 aaggaaagct tgacagctca acaggtagct gaagcaataa aaagaggctt ccaacaatcg
     6601 atagcagatg tagaatatct cctctgccct gttggtgatg ggggagaagg tactgtagat
     6661 gctatccgac attctcttga cctaaaagaa aaatggatcc aagtgacaga cccttttgga
     6721 caaaaagaag ccatgcgcta ttttcaaaaa ggggaactgg cactatttga agtagctgac
     6781 ttggttggtc ttggaaaaat tccgctagag aaacgaaatc cacttcaaat ccaaacttgt
     6841 ggtattggag agttgattct ccatctcatt tctaaaggga ttaaagatat ttatatcggt
     6901 gttggtagca cggccagtaa tgatggagga ctggggattg ctgctggttt aggttatcaa
     6961 ttttatgata gggatggaaa tgtcttgcct gcttccagtc aatccttatt aaacttagct
     7021 tctgtttcaa cagaaaattg ctataaaatt cctgaaggtg ttcaaattca tattctagca
     7081 gatgtcgtga gtcccttatg tggtcatcaa ggtgcgactt acacttttgg caatcaaaaa
     7141 ggtctacatc cgactatgtt tgcagtcgta gatcaggcga tccaagattt ttatgaaaaa
     7201 ttctcacctg caacattaga aattaaagga gcaggagcag gtggaggcct tgctggtggt
     7261 ttgtgtgcct ttgctcaggc aagtatcgtg tctggaattg ataccttgct tggacttaat
     7321 caactttgat aagaaagttt cagatgctga cttgcttgtt gttggagaag gaagacttga
     7381 cagtcaaagc tttgctggga aagcgcctat aggtgttgca aaaagaaccc ctgttggagt
     7441 tcctgttatt gctatttgcg gtagtcttgc tgaagattta cctcccctac catttgaaaa
     7501 tatccaagct gtcttttcta ttttggagaa aagtgaacct ttagaagata gtttgaaaaa
     7561 tgccagtctc tatctggagc gcacggctgc caatattggg cacttattaa atatgtgcaa
     7621 gatttagcta aaccatttct tccataagga tgttttggct ggttcttcct ttttaacttc
     7681 ccaaagtttt ggaaaagcaa gtcgaaggtc tttttcttct acttgaactg gtgcattgct
     7741 atgaataacc aggccaaaag gagaagtaat atgctcttca gatactatgg tggcttgact
     7801 atcagtttct tttgcttctt ttaagtagaa aacttgctta tcaaattcga tagttggtga
     7861 aatcttcaca aatagtggct ctgctttttc ctgaaggttt tctaaaataa ataaaaagcc
     7921 tttttctaac tgagggctat ttgctgtgtc aatatctaca tatccaagaa ctctttcctc
     7981 aaaagtacca agatagcgtc tttgctcatc cggatttatt tttaacccac catgagcttt
     8041 ttcaagtaat tgttttgata aatctgtcat gaaaacagta tatcataaaa gttagaataa
     8101 aacagacaaa agaaagcgat tactttataa agttaaggaa aagttgcaca aagataacgt
     8161 tttttcttga aaaaggaggg gttttaaggt attatagagg tgtaaaaaat ttaactcata
     8221 aggagagtaa aaaatgtcaa ttattactga tgtttacgct cgcgaagtcc tagactcacg
     8281 cggtaaccca acacttgaag tagaagttta cactgaatca ggtgctttcg gacgtggtat
     8341 ggttccatca ggagcttcta ctggtgaaca cgaagcagtt gaacttcgcg acggtgacaa
     8401 atctcgttac ggtggtcttg gtacacaaaa agctgttgac aacgtaaaca acatcattgc
     8461 tgaagctatc attggctacg atgtacgtga tcaacaagct attgaccgtg ctatgatcgc
     8521 acttgacggt actcctaaca aaggtaaatt gggtgcgaat gcaatcctcg gtgtgtctat
     8581 cgctgtagct cgtgctgctg ctgactacct tgaaatccca ctttacagct accttggtgg
     8641 attcaacact aaagttcttc caactccaat gatgaacatc atcaacggtg gttctcactc
     8701 tgacgctcca atcgctttcc aagagttcat gatcttgcca gttggtgcgc caacatttaa
     8761 agaagccctt cgttacggtg ctgaaatctt ccacgctctt aagaaaatcc ttaaatcacg
     8821 tggtttggaa actgccgtag gtgacgaagg tggattcgct cctcgtttcg aaggaactga
     8881 agatggtgtt gaaactatcc ttgctgcgat tgaagctgct ggatatgtac caggtaaaga
     8941 cgtatttctc ggatttgact gtgcttcatc agaattctac gataaagaac gtaaagttta
     9001 cgactacact aaatttgaag gcgaaggtgc tgctgttcgt acatctgcag aacaaatcga
     9061 ctaccttgaa gaattggtta acaaataccc aatcatcact attgaagatg gtatggatga
     9121 aaacgactgg gatggttgga aagctcttac tgaacgtctt ggtaagaaag tacaacttgt
     9181 tggtgacgac ttcttcgtaa caaacactga ctaccttgca cgtggtatcc aagaaggtgc
     9241 tgctaactca atccttatca aagttaacca aatcggtact cttactgaaa cttttgaagc
     9301 tatcgaaatg gctaaagaag ctggttacac tgctgttgta tcacaccgtt caggtgaaac
     9361 tgaagattca acaatcgctg atatcgcagt tgcaactaac gcaggacaaa tcaagactgg
     9421 ttcactttca cgtacagacc gtatcgctaa atacaaccaa ttgcttcgta tcgaagacca
     9481 acttggtgaa gtagctgaat atcgtggatt gaaatcattc tacaacctta aaaaataaaa
     9541 tagttcagtg aactatttta tcccgaacca tgaaattcaa aagttcggcc gttgatttaa
     9601 caacgtttct agcccctcgg attttatccg aagggctatt tttgtattta gagggcaaaa
     9661 aagggacaaa actttttggc tctttgtcaa ctgtagtggg ttgaagtcag ctaagctcga
     9721 gaaaggacaa attttgtcct ttcttttttg atattcagag cgataaaaat ccgttttttg
     9781 aagttttcaa agttccgaaa accaaaggca ttgcgcttga taagtttgat gagattattg
     9841 gtcgcttcca atttggcgtt tgaatagggt agttgaaggg cgttgacgat tttctctttg
     9901 tcctttagaa aggttttaaa gacagtctga aaaagaggat gaacctgctt tagattgtcc
     9961 tcaatgagtc cgaaaaattt ctccggtttc ttattctgaa agtgaaacag caagagttga
    10021 tagagctgat agtggtgttt caagtcttgt gaatagctca aaagcttgtc taaaatctct
    10081 ttattggtta agtgcatacg aaaagtagga cgataaaatc gcttatcact cagtttacgg
    10141 ctatcctgtt gaatgagttt ccagtagcgc ttgatagcct tgtattcatg ggattttcga
    10201 tgaaactgat tcatgatttg aacacgcaca cgactcatag cacggctaag atgttgtaca
    10261 atgtgaaagc gatcaagaac gatttcagca ttcgggagtg aaacagtctg ggagactgtt
    10321 tcagcctgag cctagaaatt tgaaagcgaa gctgtttagc caagtcatag taagggctaa
    10381 acatatccat agtaataatt ttgacgcgac atcggacaac tctatcgtag cgaagaa
//
LOCUS       DAVKMU010000055        10359 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKMU010000055 DAVKMU010000000
VERSION     DAVKMU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.055"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(308..700)
                     /gene="rpsI"
                     /locus_tag="VW343_001777"
     CDS             complement(308..700)
                     /gene="rpsI"
                     /locus_tag="VW343_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9756292.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(719..1165)
                     /gene="rplM"
                     /locus_tag="VW343_001778"
     CDS             complement(719..1165)
                     /gene="rplM"
                     /locus_tag="VW343_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9756293.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISELI"
     gene            1522..1839
                     /locus_tag="VW343_001779"
     CDS             1522..1839
                     /locus_tag="VW343_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9756294.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1990..2802)
                     /gene="folK"
                     /locus_tag="VW343_001780"
     CDS             complement(1990..2802)
                     /gene="folK"
                     /locus_tag="VW343_001780"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9756295.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2845..3399)
                     /gene="folE"
                     /locus_tag="VW343_001781"
     CDS             complement(2845..3399)
                     /gene="folE"
                     /locus_tag="VW343_001781"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9756296.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3380..4702)
                     /locus_tag="VW343_001782"
     CDS             complement(3380..4702)
                     /locus_tag="VW343_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9756297.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLESIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEEKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGCFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADH
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKARNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4704..5648)
                     /gene="folP"
                     /locus_tag="VW343_001783"
     CDS             complement(4704..5648)
                     /gene="folP"
                     /locus_tag="VW343_001783"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9756298.1"
                     /translation="MSSKANHAKTAICGIINVTPDSFSDGGQFFALEQALQQARKLIA
                     EGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDTWKSQVAEAAL
                     AAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLIFPHFGFGQTF
                     TEKELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGLTKKENLLLLR
                     DLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTASAHVTSIAARQ
                     GVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5773..6480)
                     /locus_tag="VW343_001784"
     CDS             complement(5773..6480)
                     /locus_tag="VW343_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9756299.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLMVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6533..7951)
                     /locus_tag="VW343_001785"
     CDS             complement(6533..7951)
                     /locus_tag="VW343_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9756300.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTKVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            8141..8953
                     /locus_tag="VW343_001786"
     CDS             8141..8953
                     /locus_tag="VW343_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9756301.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9292..10311
                     /gene="adhP"
                     /locus_tag="VW343_001787"
     CDS             9292..10311
                     /gene="adhP"
                     /locus_tag="VW343_001787"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9756302.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSETMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3047 a         2339 c         2020 g         2953 t
ORIGIN      
        1 agtagattga aactagaata gtacacctct acttctaaaa cattgttaga attcgatttg
       61 attatcctcg gtttgtccta ttcttatttc accttactat aaaaataagg taaacttttg
      121 agtgctttga aacattgata taatcgtatt gattaacgtt ttgaggcaac tgaccttgtc
      181 aggttgcagc cacatttgta agcgactaaa gtcgcaacaa ctgtgtcaat tgcaccaatt
      241 tagtaagaaa gtataaaaaa agaacacccc gaaaggtgct ctgtataagt atagtaattc
      301 tttcgaatta acgtttacta aattgtgatg ctttacgagc tttcttaaga cctggtttct
      361 tacgttcaac tttacgtgag tcacgtgtaa gaagtcctgc gcgtttcaat gaatcgcgga
      421 agtctgggtc tacttgaaga agggcacgag cgataccgtg acggatagct cctgattgac
      481 cagcgtatcc accacctaca acgttaacga aaacgtcgta tgaacctaca gttgaagtaa
      541 ctgcgaatgg ttggttgatg acaagacgaa ggtcagcgtg tgggatgtac tcttcaacat
      601 cttttttgtt aacagtgatt ttaccagttc ctggaacaag gcgaacgcgt gcaacagcgt
      661 ttttacgacg tccagtacct gcatattgtg cttgtgacat actttattgt tcctttcctt
      721 agataagttc tgaaatgtca agaacttctg gttgttgtgc agcgtgagtg tgctcagctc
      781 caacaaatac tttcaacttc ataccttgag cgcgtccaag tgtattgtgt ggaagcatac
      841 ctttaactga tttctcaatc aaacgtactg catttttaga acgaagttca cctgcagaga
      901 tttgtttcaa tccacctggg tggtttgagt gagtgtagta gattttatca gttgcttttt
      961 taccagtcaa tttaactttt tcagcattga taacaatcac gaagtcacct gtatcagtgt
     1021 gtggtgtaaa tgttggtttg ttttttccgc gaagtacgct agcaactact gcagaaagac
     1081 gtccaagtgg tacatcagtt gcgtcaacta cgtaccattt acgttcaact tggcctggtt
     1141 tagccataaa tgttgttttg ttcatgattt ctcctatata tagttcgatt tttgtttacg
     1201 gtgatgggtg ttccttccca tcgaaaagta tttggaaggt tccggggcct ttcaaatggg
     1261 gtaaacaata ccgcctacta tcataccaaa ttttacccct aaaagtcaat agatatgaaa
     1321 aatatttttc ggtaacttct caaagtaact gaaagttata taatcaagtt aatttgggaa
     1381 tttacctatt aacttcggta gatttttggg aatttttttc cttctttttc cctgtagtta
     1441 aaatcctttt caaaaaatca ccttttcttg atttttaatc ctatttgctg tatgataagg
     1501 gaaaagaaag gggacagaga tatggctttt accaataccc acatgcggtc tgctagtttt
     1561 ggcattgtta ccagcttgcc cgatgacatc attgactctt tttggtatat catcgatcat
     1621 ttcttaaaaa atgtctttga attggaagaa gaactcgagt ttcaattgct taataaccaa
     1681 ggaaagatta ccttccactt ttcaagtcaa cacctcccta cagccattga ttttgacttt
     1741 aaccatcctt tcgaccctcg ttatccccca agagtactgg ttttagacat ggacggtaga
     1801 gaaactatcc tcctcccaga agaaaatgac ctattttaat actcaatgaa aatcaaagag
     1861 caaactagga agctaaccgc aagctgtact tgagtacggt aaggcgacgc tgacgtggtt
     1921 tgaatttgat tttcgaagag tataaaaact ctagccttca gttgcaagtg actgaaaact
     1981 agagtttttc tattttttca aagcatcata caagttgcgg atcggttgtt ttaatatcgg
     2041 atggataaaa tgaggcgcaa tttcctgtaa ggactcaagg acaaaaaggc gttccgctat
     2101 gtaaggatga ggcaatatga ggtcgtctgt ataaaggatc tggtcctcca caaagagcaa
     2161 gtccaaatca atcaaacgag gtccccaatg cacttctctc acccgtccca gctctgactc
     2221 aatggctaac aaggtttcta acaagtcttg tgctggtagc caggtttcca cctcaaccac
     2281 ttgattggca aagctatcct gctccactcc accccaaggc tccgtcgcta agacactgga
     2341 ctctttgaga atatggatgc cacgagctcg cagtttgtca atggcttgct tcaagtttgc
     2401 ttgtttatct cccatattgc ttcctagggc gataaaggct cgttgcttgc ggcgatgaat
     2461 ggttaccgag caagtatcta gtgacaaatg caccggtgcc caaggttttt tcagttccaa
     2521 cttcatttct tggacaagag gataagactc aaaggtacgt tccaccagtt tataggctac
     2581 cgtttcaatc aaatcttcac tcgtttcctg aaaccaagtc gtccactgct gacacaattc
     2641 tccgtaatgg acagaggctg ttaaatccaa gtctgtagct gccttggtca tatcatagga
     2701 taggatggct gaaacgacaa atttctgccc caattctttc tcactaggaa aaagaccatg
     2761 ataggcaaac atttccaaat ccttaatctg cagttgatcc ataatgattc ctttctagaa
     2821 aaatccgctt gaagcggatt ctttttatag ccccattaaa cgataagctt ggtcacggag
     2881 atccttatct gtttcaaata gaccacgagc tactgtcgtc aaggttgcag tgcctggttt
     2941 tctaacacca cgcatactca tacacatatg ttccgcctca atgacaacaa aggctccttt
     3001 agcacctaga tagtccatca aggcatcggc cacttcgata ttcaaacgtt cttgaatttg
     3061 tggttttttc gaataaactt caaccgtacg ggctagctta gacaagcctg ccacacgacc
     3121 atctggaatg taggcaatgt gcgctctacc ataaaatggc aagaagtggt gttcacacat
     3181 ggtatggaaa aagatatcct tttctaccac catattatcg tcaataattt caaaggattt
     3241 tgacaaatgt tcctctgctg tttgaccaag acctgaaaaa atctcttgat acatacgggc
     3301 tacacgagca ggtgtttcct gcaagccctc gcgattagcg ttctctccta cagcctcgat
     3361 aatcattttt acagctgctt caatcttttg tgtatccatt ctcgttcttc ctctccatca
     3421 gataggccct cacttggctc aagaaataca aggaacctgt gacaatccta actgtttgtt
     3481 tctcttcttt tttatctgtc aaattctgct ctagaaaatc atgccaatct tggtagctga
     3541 gatttctagc cttagctgcc tctttcagca cgctttcatc cgtcgcccga ctatccgcaa
     3601 aatgtgttag agtaagctcg gtatctggca tggctcccag caagtccaac atatcctcca
     3661 aggctttggt tttgatacaa gtgaagagga tttccttatg atgatccgca aaacgttctt
     3721 gcaaggttac caacaaggcc ttgatagcat ggggattgtg ggctccatcc aaaatcatca
     3781 agggatctct tgacacgatt tccaaacgcc ctggccaact tgtttcttcc aaggcttgac
     3841 caagaaagtc attgcttgct agctctcgac catcttcttg acaaaaagta tcaagtaaag
     3901 ctatggccat cccagcattc tctatttggt acaaaccaag caggctagtc tggaagcaac
     3961 cttgtctgac agcacttgta tagtcaaaga cttcccctgt caccacactt tcttgatgac
     4021 gaacctgata atctgtcccg taggcaagtc tcggcgcatc tttctcttcc gcaatgcggt
     4081 caatcacagc taaggcttct ggagcaatac gccctgttac caagggcatc ccttgtttga
     4141 taatacctgc cttctgctct gctatggact ccaaggtgtc accaagtagg gccacatgat
     4201 ccaagccaat agttgtaatt cctgtcaaaa tgggctgaca gacattggta ctatccaaaa
     4261 gtccacccat gccaacttcc atgatggcca catctacttg ctctgaggca aagtagtcat
     4321 aggccagggc tgtgataatc tcaaactcgg ttgtgccctg taaattggcg accgcttctc
     4381 cctccagcaa agactgatag tctgccatga gagcttctag cctcgcttct gagatcgatt
     4441 ccccattgat gctaatctgg tctgtgtaat gaatgagata gggcgagcta aacacgccaa
     4501 ctctcaaccc tagcttttct agcatctttt tcaaaaaagc aatagtcgag cccttgccgt
     4561 tagtccctcc gatatggagg accttgagtt tgagatgggg attgccacgc aaagctaaca
     4621 gttccaccat tcgttccaag ccaaaatgcg gttgatccgt ccggtagtta gcaatccact
     4681 gattgttttc aatttctttc atcttattta tattgtttta aatctaaatt ttccgcttca
     4741 tcagccagac gaatggcaga ggcaatttca actgccatcc tgtgactagc tacgtcatgc
     4801 acgcgcacca cttctacacc ctgtctcgca gcgatactag ttacatgagc cgaagccgtg
     4861 tcccgatttc ggaaaccaag ctctgtctca ggattgactt caaaaccatt ctcctctagg
     4921 atattgatga caaatcgctt gcgcgacact ccgagaaaga ttggatagcc cttctgatgt
     4981 agtttatcca ggtcccgtaa aagaagcaga ttttctttct tggtcagacc aaagccaatt
     5041 cctggatcca acaggatatt ttctggtgca ataccagctt ctgccgctct cgctagtgct
     5101 cgttcaaaga aagcctccat caattcttcg attggcaatg tttcaaagtc cgctaactct
     5161 ttttctgtaa aggtttgacc aaaaccaaaa tgagggaaga taagcgaact aggatgctga
     5221 ggccgagcca taactgggtt aaacatgatg accacttgcg ctctcgcttc agctaccaca
     5281 tgagccattt tctcatcacc cataagacca gtgatatcat tgactagatc ggcaccagca
     5341 gccaaagcag cctctgctac ttgactcttc caagtatcaa tagagatgag gacatcactt
     5401 tccttgcgaa tcgctttgat cactggaaca acacgctgga tttcctcttc tatctcaaca
     5461 tagctactgc ccggccgagt cgattctccg ccgatatcga gcatactggc tccttctgct
     5521 atcaatttac gagcctgctg gagtgcctgc tcaagagcaa aaaattgacc accgtccgaa
     5581 aaggagtctg gggttacatt gataattccg caaatagctg tttttgcatg attggcttta
     5641 cttgacatat cggtcactcc ctcaaggctt ttcatcttat tatttttcta ttttaccata
     5701 aaaagaaaaa gatggacacg attgcattca tctttcccta gtagaaagag gtcggtttct
     5761 acataaaagc ttttaaacag aaattcctaa tgtccgactc ataatcacca caagagccaa
     5821 caaacagaaa gcaatcccat taacaatcat gtgaagcaag atcgacattt ccaaacgttg
     5881 ggtcttgtag gccgtccaag atagaactgt cgacatacct ccataaatca ataaagaagg
     5941 taaattactt ggttgatgca ataaagcaaa cacaatcgta ccgactacaa atcccaagtt
     6001 ctccttgcct cggaaaatct ttttaggaac aatcccacga cacaagattt cctcacaaat
     6061 cggagcaagc aaggctagca agaagaaact ggaaatcaac gaactatttt gaaccatatc
     6121 attaatctga gactggttag ctgttgtcgt ctcatttgac agttgcaaca agatagaacc
     6181 aagtatattt gacccgacaa taactaaata actcaaaccc aaacgtgcca aatctttagc
     6241 tctaaaaaaa gaaaaattaa aactagctaa cttggtttta cgagctccca taataaatag
     6301 agccagaacc acaattgaaa ggccagcaac catcagccct gactgtagca gggctacttc
     6361 ttttaaagtc aaaatagagg taaccactag gggaacctga tataaaagga cggcaagtaa
     6421 aaagaccaaa atccagcctg cacgattcaa caattctttc cacatatttt tctctttcat
     6481 cacctatctc ctttgtatta aaaaaggggg gaaatccccc tgggtcattc tattataagg
     6541 ccatgctaat gtagttaagg ataaacaagg catccaaaat ccaaatcatg gcatgaacat
     6601 ctttaacttg acctttaaca agcttagtca aagtgtaagt caagaaacca actgcaatcc
     6661 cttgagtgat agagtagctg aatcccataa agatagatgt gaagaaggca ggaactgctt
     6721 cagacatatc atcccaatgg atatttttca agctaccaag catcataatc ccaacgataa
     6781 tcaagattgg agctgtagcc gctgttggta cgatcgctag aagtgggcta aagaagcttg
     6841 aaatcgcaaa acagatagct acaaccaagg ctgtcaaacc agtacgtcca cctgcaccga
     6901 taccagcagc agactcaaca taagtcgtta cgtttgaagt acctgcaatg gcaccgactg
     6961 tcgttccaat caaatcagag taaagagcct tatccaattt ggctgattgg tgattttcac
     7021 catttgtcgc tacaatacca actttttcac ctgtaccgat caaggtacca attgtgtcaa
     7081 aaatatctgt caatgagaag gcaagaatgg ccatcagagt ttcaggcaag cgagctgtat
     7141 ctgaaaccaa agctcctaaa ccttctgaac caagagctgc accaaagatt gtcttcaaat
     7201 cttcaaaagc tgcaccaaca tggttattag caaaatcgat actagacaaa tcaaccaaac
     7261 caactgcaat agcaagaaca gttgttgtca agatagagag aataattccc cctttaatcc
     7321 ctttgatgac aaagaagata gtaatggcaa gtcctgcaag agccactaaa acagctggat
     7381 tattaaagct gaccaatcct ggaactgctg aagagtttgc tgcaatcgtt gcttgagctt
     7441 tgtcagcccc ttctcctaca acagtatagt tgcctggatc aatcgtgaat ttcaaaagtc
     7501 cagcattctt aatccctaca taggcaagga agacaccgat accagctgaa atagctgagc
     7561 gaagagcatt gggaatcgat tcaatgatca ttttacgaac ctttgtcaag gtaataatca
     7621 atgaaataat cccacagatg aagaccatag ctagggcttc ttgccaagaa taaccaagcc
     7681 cgaatacaac tgtaaaggta aagaaggcat tgagtcccat acctggcgct tgggcataag
     7741 gtaagttagc ataaaaagcc atcatcaagg tacccgctac tgcaccaata atcgtcgcta
     7801 ggaagacacc ctgagcaggc attcctgttt gtgaaagtat ttgtgggttt acaaagagaa
     7861 tatagctcat tgcaaagaaa gttgttaaac cagcgagaac ctctgtacga acgtctgtac
     7921 cgttctcttt tagtttaaat aatttgtcca tcatcaatct ccttttaatt tttacgaaca
     7981 ataatagaat tatatcattt atcattcact ttttcaatgt ctttgtttga tttgtttaag
     8041 aaattaatcc aatttcattt tttgtttgga atctgctttt ctgcctgctt tctgttataa
     8101 tagaagacat cttatctgaa aacacactag aaaggtccat atgacgaaaa aaattattgc
     8161 agttgacctg gatggaaccc tgctcaactc agacagtcaa atttctgact ttaccaaaaa
     8221 aaccatcaaa aaagttgctg aaaaaggcca tcaggttatt attacgacag gtcgccctta
     8281 ccgtatgtca aaagattttt accgtgaact gggcttagac actcctatga ttaacttcaa
     8341 cggatccctt actcatttac cagaccaagt ttgggatttt gaaaagtgtt tgactgtaga
     8401 caaaaaatat ctgctagata tggttcaacg ttcagaggac attcaagccg attttatcgc
     8461 tggagaatat cgtaaaaaat tctacattac aaatcccaat gaagagattg ccaatcccaa
     8521 actatttggt gtagaagctt tccagcctga agatcaattc cagcctgaat tggtgaccaa
     8581 ggaccctaac tgtatcctct tgcagaccag agccagtgac aaatatgcct tggcaaaaga
     8641 catgaacgcc ttctaccagc atcaactttc tatcaatacc tggggaggtc cgctcaatat
     8701 ccttgaatgt accccaaaag gtgtcaacaa ggcctttgct ttggactact tgctcaagat
     8761 aatgaatcgt gacaaaaaag atttgattgc ctttggagat gaacacaatg ataccgaaat
     8821 gctcgctttt gctgggaagg gttatgccat gaaaaatgcc aatccagagc tacttcctta
     8881 tgcagatgag caaatttccc ttaccaacga ccaagatggg gttgccaaaa ccctacaaga
     8941 cttattctta taacctatac tgatactcaa tgagaatcaa agagcaaact aggaagctag
     9001 ccgtaggttg ctcaaagcac tgctttgagg ttgtagatga agctgacgtg gtttgaagag
     9061 attttcgaag agtatgacca gcttgcaagc tggtctttgt gctcttttca cagtctcatc
     9121 aaccagttaa tcgattgttc tactttttgt ctccaaaacc ttggaaaagg ctttcttttg
     9181 tgatatactt cacatataaa tacaagagag ctcgtcacac tcgactctgt tgacaaaaaa
     9241 tcaatccagt cccgttgtcc ctgttctcgg tcaatatcaa ggaggatagc tatgaaagct
     9301 gttgttgtaa atccagaaag cactggtgtt gctattgaag aaaaagtact ccgtccactt
     9361 gaaactgggg aagcacttgt agaagttgaa tactgtggcg tttgccacac cgacctccac
     9421 gttgctcatg gtgactttgg tcaagtccca ggacgtgttc ttgggcacga aggtatcggt
     9481 atcgttaaag agattgctcc agatgtgaaa agccttaaag tcggtgaccg cgtcagcgtt
     9541 gcttggttct ttgaaggatg tggcacttgc gaatactgta caactggtcg cgaaaccctt
     9601 tgccgtacag tgaaaaatgc tggctactca gtagacggtg gtatggctga acaatgtatc
     9661 gtaactgctg actatgctgt caaagttcct gacggacttg atccagccca agcttcttct
     9721 atcacatgtg ctggagtaac aacctataaa gctatcaaag aagcaaaagt tgaaccaggc
     9781 caatgggttg ttctttacgg tgctggtgga cttggtaacc tcgctgttca atacgctaaa
     9841 aaagtattca atgctcatgt tatcgcagtc gatatcaaca atgacaaact tgcccttgca
     9901 aaagaagtag gcgctgacat tgtgattaac ggcctcgaag ttgaagatgt agctggactc
     9961 attaaagaaa aaactgatgg aggagctcat tcagctgtcg taactgctgt gtctaaagtt
    10021 gccttcaacc aggctgttga ctccattcgt gctggtggtc gcgtcgtcgc tgttggtctt
    10081 ccttctgaaa cgatggaact cagcatcgtt aaaacagtcc tcgatggaat ccaagtcatc
    10141 ggttctcttg tcggaactcg taaagactta gaagaagcct tccaatttgg tgcagaaggt
    10201 ctggtagtcc cagttgttca aaaacgtcca gtagaagatg ctgttgccat tttcgacgaa
    10261 atggaaaaag gccaaatcca aggacgtatg gtactcgact tcacccacta atctaataga
    10321 aacctatttt aaaagttgga agcttcttcc aactttttt
//
LOCUS       DAVKMU010000056        10321 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKMU010000056 DAVKMU010000000
VERSION     DAVKMU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.056"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            125..3226
                     /locus_tag="VW343_001788"
     CDS             125..3226
                     /locus_tag="VW343_001788"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9756303.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQKSEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQVDD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSDQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            3308..4315
                     /gene="pfkA"
                     /locus_tag="VW343_001789"
     CDS             3308..4315
                     /gene="pfkA"
                     /locus_tag="VW343_001789"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9756304.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     L"
     gene            4374..5879
                     /gene="pyk"
                     /locus_tag="VW343_001790"
     CDS             4374..5879
                     /gene="pyk"
                     /locus_tag="VW343_001790"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9756305.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6613..6838)
                     /locus_tag="VW343_001791"
                     /pseudo
     CDS             complement(6613..6838)
                     /locus_tag="VW343_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7686..8558
                     /locus_tag="VW343_001792"
     CDS             7686..8558
                     /locus_tag="VW343_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9756306.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            8571..8735
                     /locus_tag="VW343_001793"
     CDS             8571..8735
                     /locus_tag="VW343_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756307.1"
                     /translation="MTVILGFLFLFLMTWWVISIVNSQKPLKNETFIGYVQRYDIDGN
                     GYQSLPCPQN"
     gene            complement(8704..9048)
                     /locus_tag="VW343_001794"
                     /pseudo
     CDS             complement(8704..9048)
                     /locus_tag="VW343_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9191..9502
                     /locus_tag="VW343_001795"
     CDS             9191..9502
                     /locus_tag="VW343_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756308.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            9506..10222
                     /locus_tag="VW343_001796"
     CDS             9506..10222
                     /locus_tag="VW343_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9756309.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
BASE COUNT         3199 a         1782 c         2191 g         3149 t
ORIGIN      
        1 aatgatgaca aaaatacaaa tgaatagaaa ataaattagt aagctgatga aatttttctc
       61 aagagaagcc atttataggt gaaaatggta taatatagtg agaaggatag aggagaagtg
      121 taaattgatc gcacaactag atacaaaaac agtctatagt tttatggaaa gcgtcatttc
      181 gatcgaaaag tatgtgagag cagctaaaga atacggctac actcatttgg ctatgatgga
      241 tattgacaat ctttatggcg ctttcgactt tctagagatt acaaaaaaat acggcattca
      301 tcctttgcta gggcttgaaa tgacagtgtt tgtagatgat caggaagtga atttgcgctt
      361 tttagctcta tctagtgtgg gctatcagca gttgatgaag ctttcgacag ccaagatgca
      421 gggggagaaa acttggtcag tcctgtccca gtacctggag gatatcgcgg tcattgtgcc
      481 ttattttgat agagttgagt cgttagaact aggctgtgat tactatatag gggtttatcc
      541 agaaacacta gcaagcgaat ttcatcatcc tatcttacct ctttatcggg tcaacgcttt
      601 tgaaagcagg gatagagaag ttcttcaagt tttaacagcg attaaagaaa atctaccgct
      661 cagagaagtt cccttgcgtt cgagacaaga tgtctttata tcagcaagtt ctttagagaa
      721 actattccaa gagtgttttc cgcaagcttt ggacaattta gaaaagctta tttcaggcat
      781 ttcttacgac ttggatacta gtctgaaact gcctcgtttt aatccagcta gaccagcagt
      841 agaggagttg agagagcgtg ctgaactggg gcttgttcag aaggggttga ctagtaaaga
      901 atatcaagat agactagacc aagaattgtc tgttattcat gatatgggct ttgatgatta
      961 tttcttggtt gtttgggatt tgttgcgttt tggacgatcg aatggctatt atatgggaat
     1021 gggaaggggt tctgcagtag gcagtttggt ttcttatgcc ttagacatca cggggattga
     1081 cccagtagag aaaaatctga tttttgaacg ctttcttaat cgtgaacgct ataccatgcc
     1141 tgatattgat attgatatcc cagatattta tcgtccagat tttatcagat atgttggtaa
     1201 taaatatggt agtaaacatg cggcacaaat cgttactttt tcaacctttg gagccaagca
     1261 agctcttcga gatgtcttga aacgctttgg tgtgccagag tatgaattat ctgcaattac
     1321 taagaaaatc agttttcgtg acaatcttaa gtcggcctat gagggcaatc tccagtttcg
     1381 tcagcaaatc aatagtaagt tagaatacca aaaagctttt gagattgctt gcaagataga
     1441 gggctatcca agacaaacct ctgtccatgc ggctggtgtt gtaattagtg accaagattt
     1501 aaccaactat attcctctaa agtatggtga tgaaattcca ctgactcagt atgatgctca
     1561 tggagttgag gctagcggac ttttgaagat ggactttctg ggactacgaa atttgacctt
     1621 tgtccagaag atgcaagagt tgcttgctga aatagaaggt attcacctta aaattgaaga
     1681 aatagatttg gaagacaaag aaacgttagc tttatttgcc tctggaaata caaaaggtat
     1741 ctttcaattt gagcaaccag gtgccattcg tctgcttaag cgtgtgcaac cagtctgttt
     1801 tgaagatgtc gtcgcgacta cttctctaaa tcgaccgggt gctagtgact atatcaataa
     1861 ttttgtggca agaaagcatg ggcaggaaga agtgactgtt ctggatccag tactggagga
     1921 tattttggct ccaacctacg gcataatgct ctatcaggag caggttatgc aggttgccca
     1981 gcgatttgcc ggatttagtc ttgggaaagc cgatattttg cgtcgggcta tggggaaaaa
     2041 ggatgcctct gccatgcatg agatgagggc ttcctttatt caaggttcat tagaagctgg
     2101 tcatactgtg gaaaaagcag agcaggtctt tgatgttatg gagaagtttg caggttatgg
     2161 ttttaacagg tcacacgcct atgcctactc agccttggcc ttccagttgg cttattttaa
     2221 aacacattat ccagccattt tttatcaggt catgttaaat tcttccaaca gtgattactt
     2281 aatagatgca cttgaagcag gttttgaagt agcctctcta tccatcaaca ccattcccta
     2341 tcacgataaa attgccaaca aggccatcta tctaggtttg aaatccatta aaggagtcag
     2401 taatgattta gctctctgga ttattgaaaa tagaccttat tctaacattg aagattttat
     2461 agctaaatta cctgagaatt atctgaaact tcctctgcta gaacctttgg taaaagttgg
     2521 tcttttcgat tcatttgaaa aaaatcgtca aaaagtattt aataatttag ctaatctatt
     2581 tgaatttgtg aaagagttgg gaagtttgtt tggagatgct atttatagtt ggcagaaatc
     2641 ggaagattgg acggaacaag aaaaatttta tatggaacaa gagcttttag ggataggtgt
     2701 cagcaaacat ccactacaag ctattgcaag taaggctatt tacccgatta ccccaatcgg
     2761 aaatttgtca gaaaatagct atgctattat tttggttgaa gttcagaaaa taaaagtgat
     2821 tcgtaccaaa aagggtgaaa atatggcttt cttacaggtg gatgacagta agaaaaaatt
     2881 ggatgttaca ctcttctcag atttatatcg acaggttggt caggaagtaa gagaaggagc
     2941 attctactat atcaaaggga aagtccaatc acgtgacggt cgtctgcaaa tgattgcaca
     3001 agaaataaga gaagcagttg ctgaacgctt ttggattcag gttaaaaatc atgactcgga
     3061 tcaagagatg tcacgtattt tagaccagta taaaggccca atcccagtca tcatccggta
     3121 tgaagaggaa cagaaaacca tcgtttctcc ccatcatttt gtagctaaat ctgatgaatt
     3181 agaggcgaaa ttgaatggaa tagttatgaa aacgatttat cgctaaaatt acggaaaata
     3241 gaagaatttt caacgtaaat gtggtataat cagtaagaat gttaaaagaa aaaggagcat
     3301 aaccaatatg aaacgtattg ctgttttgac tagtggtgga gacgcccctg gtatgaacgc
     3361 tgccatccgt gcagttgttc gtcaagcaat ttcagaagga atggaagtgt ttggtatcta
     3421 tgacggatat gctggtatgg ttgccggtga aattcatccc ctagatgcag cttcagtagg
     3481 ggacatcatt tctcgtggtg gtactttcct tcactcagct cgttacccag agttcgctca
     3541 acttgaaggg caacttaaag ggattgagca attgaaaaaa cacggaattg aaggtgtagt
     3601 tgttatcggt ggtgacggat cttaccacgg cgctatgcgt ttgactgaac atggcttccc
     3661 agctattggt cttccaggta caatcgataa cgatatcgtt ggtactgact ttacaatcgg
     3721 ttttgacaca gcggttacta ctgccatgga cgctatcgat aagattcgtg atacatcatc
     3781 aagtcaccgt cgtacttttg taatcgaagt tatgggacgt aacgctggtg atatcgctct
     3841 ttgggctggt attgcaactg gtgctgatga aatcatcatc cctgaagcag gcttcaagat
     3901 ggaagatatc gtagcaagca tcaaagctgg ttatgaatgt ggtaaaaaac acaatatcat
     3961 cgtcttagct gaaggtgtga tgtcagcggc tgaatttggt caaaaactta aagaagctgg
     4021 agatacaagc gaccttcgtg taacagaact tggacatatt caacgtggtg gttctccaac
     4081 tgcgcgtgac cgtgttttgg cgtcacgtat gggtgcacat gctgttaaac ttcttaaaga
     4141 aggtatcggt ggtgttgcgg ttggtattcg taacgaaaaa atggttgaaa atccaattct
     4201 tggtactgca gaagaagggg cattgtttag ccttactgca gaaggtaaga ttgtggttaa
     4261 caacccacac aaagccgata ttgagctatc tagcttgaat aagagcttgt tataatttac
     4321 aatttagtta ataagaccaa aaaggtcata tatataaagg agtcacaaaa atcatgaaca
     4381 aacgtgtaaa aatcgttgca actttgggtc ctgcggtaga aatccgtggt ggtaaaaaat
     4441 tcggtgagga cggatactgg ggtgaaaaac ttgatgttga agcttcagct aaaaacattg
     4501 ctaaattgat tgaagctggt gctaacacat tccgattcaa cttctcacac ggcgaccacc
     4561 aagaacaagg tgagcgtatg gcaactgtta aacttgcgga aaaaattgca ggtaaaaaag
     4621 ttggtttcct tcttgataca aaaggacctg aaatccgtac agaattgttc gaaggtgaag
     4681 ctaaagaata ttcatacaaa actggtgaaa aaattcgtgt tgcaactaaa caaggaatca
     4741 aatcaactcg tgaagtgatt gcgttgaacg ttgctggtgc tcttgatatc tatgatgatg
     4801 ttgaagttgg tcgtcaagtt ttggttgacg atggtaaact tggtcttcgt gtggttgcta
     4861 aagatgatgc aactcgtgaa tttgaagttg aagttgaaaa cgatggtatc atcgctaaac
     4921 aaaaaggtgt gaacatccct aacactaaaa ttcctttccc agctcttgct gaacgcgata
     4981 acgacgatat ccgtttcggt cttgaacaag gtatcaactt catcgcaatt tcattcgtac
     5041 gtactgcaaa agatgtgaac gaagttcgtg caatctgtga agaaactgga aacggacatg
     5101 ttcaattgtt cgctaaaatc gaaaaccaac aaggtatcga taacttagac gaaatcatcg
     5161 aagcagctga tggtattatg attgctcgtg gtgatatggg tatcgaagta ccgttcgaaa
     5221 tggttccagt ttatcaaaaa atgattatca agaaagtcaa tgctgcaggt aaagttgtta
     5281 tcactgcaac aaacatgctt gaaacaatga ctgaaaaacc acgtgcaact cgttcagaag
     5341 tatcagacgt attcaacgct gttatcgacg gaactgacgc tacaatgctg tcaggcgagt
     5401 ctgcaaacgg taaataccca ctcgagtcag taactacaat ggctacaatc gacaagaacg
     5461 ctcaagctct tcttaatgaa tacggacgtc ttgattcaga ttcatttgag cgtaactcta
     5521 agacagaagt aatggcttct gctgttaaag atgctactag ctcaatggat atcaaattgg
     5581 ttgtaactct tactaagaca ggtcatactg cacgtttgat ttctaaatac cgtccaaatg
     5641 ctgacatctt agcattgaca tttgacgaat tgacagaacg tggcttgatg ttgaactggg
     5701 gtgttatccc aatgttgaca gatgctccat cttcaactga cgatatgttc gaaatcgctg
     5761 aacgtaaagc ggtagaagca ggtctcgttg agtcaggcga tgatatcgtt atcgttgctg
     5821 gtgtgccagt aggagaagct gttcgcacaa acacaatgcg tatccgcaca gtacgttaag
     5881 aaaaatataa aaacctatca tatccagctt tctagcctgt atgatgggct ctttttttgt
     5941 ccagggggca aaaagggggc aaaactttca taatggattg atactcttcg aaaatctctt
     6001 caaaccacgt cagcgtcgcc ttaccgtata tatgttactg acttcgtcag ttctatctac
     6061 aacctcaaag cagtgctttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
     6121 cattgagtat gaaataagat atgcacaaat tgattaggaa attcaaatga atttctacaa
     6181 atgttttagc aatcgcaatc tacttgtcta gattcgatct gatatatttt cgatttaatg
     6241 atatggtatt taaaacctcc aaagtagctt gctccattct tttacttacg tgagtgtaga
     6301 tgttatttac tgttttagcg tttttgtgtt ctactctaac cattatagca ttcttctcag
     6361 ctagtgtact aaggagtgtg tgcctgaaaa tatgggaact aagggctggt ttatcggttt
     6421 ctctagttta gtatttgcct tttgcaaagt gatcttaaat gcctttctct aaatttacat
     6481 atcactattg tttaacaaaa tctaatctac tttaggtcac ttattctttt tttgaaatgt
     6541 agaatgaact ttttcaaagt ttttcgaatc ttttaaaatc tgtttgtttt atatcgccat
     6601 tctccccctt ttttaattct ccctatatag cctgacaact ttcccgatgg tacgaatatg
     6661 gttgctttcg tctaggtgga tgtcggagta ttcgggattg agtttttttg aggtagcctt
     6721 ggcggagttt cttgacatag ttagtgccgt ctacttggaa gatgccgatg gtattatagt
     6781 caatctgtgg ggtattcttg ataaataggt agtcgctgtt tcttatcttt ggctccatgg
     6841 acttgctgac gacataagcg attgggtcgt agtcgtctgg gataatggaa actccataac
     6901 taaatcgtcg tcctgcatca agcggctacc tgcagagata aactacctaa cacgagagta
     6961 agtagtctgt ctgtagtcgt ccagtctgat gatttttacg atacttcgtt tttctgatca
     7021 tacagttgcc tctcggcata ggtcagaact ttaccttgtc tgggtggtgc ccgttggtcg
     7081 tagatagatt ggatatcgct aggagaatcc ttttgaactg gaggaaagag ggcatcaatc
     7141 aagctactga atactttaac taagtcaaat atagtatttt tcttagtaga cctaaccctt
     7201 ttttcataat ttctaatggt gtttttactt atacctatct tagtacccaa ttcttattga
     7261 gtccaaccat tactagtcta tattgtttta tagttgattg agtttggaat agtacgctgt
     7321 agctgctaaa acatttctag gaattaattt gcctttccta atagagttgt tcatatctta
     7381 tttcaatcta ctatattttt cacctctaac aatcgcaatc tcttctttat ccatgaacga
     7441 aaacgctttc tatctttgta agtaaagcat cacacgaaat ccacgaaaat gaaaaccttt
     7501 gttgtgtttt cgtaaaaaat ttgttgacag agcacgaaac gcctactata attaaatcaa
     7561 gtttaaggaa ataacaaaaa aacaaatcgg agggaaagac gatgaacaca ttaaatgaga
     7621 aagtaatcaa tatctgtaaa gcagtagtta aagaaacttt aatccaagac atttaggaga
     7681 aagaaatgaa gaaatcaaga aaattagcca cattaggaat ctgttcagcc ttgtttttag
     7741 gcttggctgc ttgtcaacaa caacatgcta cttctgaggg gacgaatcaa aggcaaagca
     7801 gttcagcgaa agttccatgg aaagcttcat acaccaacct aaacaaccag gtaagtacag
     7861 aagaggtcaa atctctctta tcagctcact tggatccaaa tagtgttgat gcatttttta
     7921 atctcgttaa tgactataat accattgtcg gctcaactgg cttatcagga gatttcactt
     7981 cctttactca caccgaatac gatgttgaga aaatcagtca tctctggaat caaaagaagg
     8041 gcgatttcgt tgggaccaac tgccgtatca atagttattg tcttttgaaa aattcagtca
     8101 ccattccaaa gcttgaaaag aatgaccagt tgcttttcct agataatgat gcgattgata
     8161 aaggaaaggt ctttgattca caagataagg aagagtttga tattctattt tcgagagttc
     8221 caactgaggc aactacagat gtcaaggttc acgctgaaaa gatggaaaca ttcttctcac
     8281 aatttcaatt caatgaaaaa gctcgaatgc tgtctgtagt cttgcacgac aatctggatg
     8341 gcgagtatct gtttgtaggt cacgttgggg tcttagtacc tgctgatgac ggtttcttat
     8401 ttgtagagaa attgactttc gaagaaccct accaagcgat taaatttgct agtaaggaag
     8461 attgctacaa gtatttgggc accaagtatg cggattatac aggcgaggga ctggctaagc
     8521 cttttatcat ggataatgat aagtgggtta aactttaata gtgttatgat atgacagtaa
     8581 tcttaggatt tttatttcta tttttgatga cttggtgggt tatttcaatc gttaatagcc
     8641 agaaaccact caaaaatgaa acatttattg gctatgtcca aagatacgat attgatggga
     8701 atggctatca atcattaccg tgtcctcaga actgagagga gttcttgaaa tcgtaacacc
     8761 actttgaaca agagttactt caacccattg gctccgccgg agtaagttgc tttcgtgaac
     8821 accaaaatca gccgcaattt cttcataagt gcggtattct cacacatatt gaagagtggc
     8881 cataagaagg tcttctaggc ttaatttagg ttttcgtcca ccttttgcgt gtttaagttg
     8941 ataagctgtt tttaatacag ctaacatctc ttcaaaagtg gtacgctgaa caccaacaag
     9001 acgcttaaat cgtgcatcag ttagttgttt acttgcttca tcattcatag aactactata
     9061 ccatattttg tttcgcagga agtctaatgc taaaatctta tcagtgagaa gatttcttaa
     9121 agtttattct ttgtagtttc atctgtatta gtacataatt taacttagag aaaataggag
     9181 gactaatcga atggaattaa aagattttac agaaaaagaa caggaaatga ttaagaaaag
     9241 gcttacaatg tctaatatta gtgacaaaga aactactgag aagattctgg cgctagtacc
     9301 acaagacttg attaagcgaa tcccgttttt tgtcagaaaa catgctacaa cacgtacgat
     9361 taaacgcatt tcaattgaat accctgaact ctacgctgta gctcaaacaa gtggtgagat
     9421 tccagaaaaa gaacgcgaag aattgcgtca gattatcaca actatctttg aacaaaagat
     9481 gaataagcat agtattaagt agagaatgaa aaaatatttt attagcggtt tgggaagcaa
     9541 tgcctatcat agcaaggatt tttttcaaga actagattcg caggtctatt ttctaaatcc
     9601 atatgaaaag catcttcgag atgaaacaga attgaaatca tggtttaaaa atgagattgt
     9661 agaggaagaa tctatctgtc tgataggcca ttctcttgga ggagatttag ctcgttattt
     9721 cgcatcggaa tttgaagaag taaagaaact gattcttttg gatggtggct atctagattt
     9781 agataagatt ttacctttgg atacagagtt agaggaaact aaaaattata tcaaatctca
     9841 aattgtttcg gacttagctc ttcttatttc taaagagaag tccgaagcaa agcattggtc
     9901 agaaaatatg gagaaagctg taagacagtc ctatcactgg aatgttgagt ataatagata
     9961 tgagttggct ataaattatg aaaatataga agcgatactc cgcctacgga gaaaaataca
    10021 agcttttaag agagaagtgg gagatacctt gtttattagt cctcgctatc ctaatggagc
    10081 tacatggaga gaggaagccc taaaagaatt gccagactat tttaatacta tttttttaga
    10141 aaactttggc catgagcttt atactcaagc acctaaagaa atcgctagtc taatgaatga
    10201 gtggctcgct tattttctat gagctacttg tattgctgga aatggtataa gatgaaaatc
    10261 tacaggctct ataatatttg tagtgggtaa atccactata gatattatgg agcctatttt
    10321 t
//
LOCUS       DAVKMU010000057         9931 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKMU010000057 DAVKMU010000000
VERSION     DAVKMU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.057"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(71..307)
                     /locus_tag="VW343_001797"
     CDS             complement(71..307)
                     /locus_tag="VW343_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9756310.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(375..1295)
                     /locus_tag="VW343_001798"
     CDS             complement(375..1295)
                     /locus_tag="VW343_001798"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9756311.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            1493..1897
                     /locus_tag="VW343_001799"
     CDS             1493..1897
                     /locus_tag="VW343_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756312.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            2113..2694
                     /locus_tag="VW343_001800"
     CDS             2113..2694
                     /locus_tag="VW343_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9756313.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETSGDLTIVIQKKQ
                     GAPSAKSKMEGVFGNCEILKKDKGYYILRSVKE"
     gene            2691..3968
                     /locus_tag="VW343_001801"
     CDS             2691..3968
                     /locus_tag="VW343_001801"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9756314.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILVPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            3986..4648
                     /gene="deoC"
                     /locus_tag="VW343_001802"
     CDS             3986..4648
                     /gene="deoC"
                     /locus_tag="VW343_001802"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9756315.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            4635..5024
                     /locus_tag="VW343_001803"
     CDS             4635..5024
                     /locus_tag="VW343_001803"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9756316.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            5114..6166
                     /locus_tag="VW343_001804"
     CDS             5114..6166
                     /locus_tag="VW343_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9756317.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            6310..7845
                     /locus_tag="VW343_001805"
     CDS             6310..7845
                     /locus_tag="VW343_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756318.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            7838..8896
                     /locus_tag="VW343_001806"
     CDS             7838..8896
                     /locus_tag="VW343_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756319.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            8899..9855
                     /locus_tag="VW343_001807"
     CDS             8899..9855
                     /locus_tag="VW343_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756320.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         2821 a         1757 c         2294 g         3059 t
ORIGIN      
        1 gataattgcg attttcattt tctacctttt cctatttgga gacaaaaaaa cagcctctat
       61 ggactgtttc ttatttagca agtttagctg aaagacgagc tttatcgcgg cttgctttgt
      121 ttttgtgaat caaaccttta gtttctgctt tatcgatagc tgagctagca gcacggaaaa
      181 gttcttcaga tgggtttgct tcgaaagctt ttatagcagt acgcatagct gatttttgag
      241 ctgagttctt ttcgttttgt ttaacgttca attcagcgcg tttgatagct gatttaatgt
      301 ttgccaatgt tcttacctcc atatttacta actataccat tatatatgaa aacttacgtt
      361 ttgacaaggg aaaattattt ttttaagtaa atttcatcga tttcatggtt cttgctttta
      421 tgaagaatca cttctgcacg atttctggtt ggttcaatat aattttgtag atttgtgaga
      481 ttgatactgg tccagacctg atgggcaaag gcttccactt ccccaatcgg catctgagta
      541 aaacgataat agtagctatc agggtcgttt tgggctagac tcagcatttt caagaaacgg
      601 tccagatacc aactttcaat atcatccact ccagcatcta catagatgga aaagtcaaag
      661 aagtcagtga tatagagacg atcgttttgt ggattttgaa agacattgat tccctcaaca
      721 attacaaaat cagcagcttt gacactttgt ttctcttcgg gtacgatgtc gtaaacttca
      781 tgagaataga caggaatatc tacatcttgt ccatttttga tgcggtccaa gaagttgaga
      841 agagcttcca tatcatagct ttcaggaaat cctttacgat ttaaaatccc ctgctcaatc
      901 aaggtttgat tgggatagag aaaaccatca gttgtaacca actcaaccgt agcatctgta
      961 aacgtacggg acagtaggat ttgaagtagg cgactggttg tggattttcc aacggcaaca
     1021 ctcccagaaa ccccaataat aaaaggttga gatttacttt cacgttggag gaaaattcct
     1081 tttgaaaagg ctaaatcttc cttagttcgc ttgtaaatct gaatcagatg agccaagggg
     1141 agatagatat ctgtaacgtc ttggagactg atttggtcat taaaactctt gatagattcc
     1201 aattcttctt ctgtcaaagg aggtgttgtc tttcgatgta aagattgcca agtctggcgg
     1261 ctgatttttt caaaatgtaa aaattcgttg gtcatttgtt ttcccctaga tattttgtct
     1321 atcataccat aaaaatctaa aaaaataaag cggtttcttg aatgtggtaa gtcaatggta
     1381 tatagtataa gtgatcaatt tattgatgaa ataggagata gattctactg atttataagt
     1441 ttataagctt ataaaggggg ctttttagtt cacaacttat aggaggtgta ctatgaaaat
     1501 cttaaaacgt tacatattgg aactctgttt tattttaagt tttgctttac cttttataaa
     1561 aggagccaat gcagataatg gtagatgctt tgtggaaacc tattacggtt ttactttttt
     1621 gatggaacat gctattgtaa cagctgtctt tatctattcg tttttaattg ctttcttact
     1681 aaaaaaacga tgggcgaaat ggattgctgc tggtagttat tgctttttag tcctatggat
     1741 tgcgacagag ggttatttct tccgcatgtc actagaagat ttgatacgac tttggacaag
     1801 tttggaaatc ctgacacaaa cgtatcagtt gggcttttat ctaaacattt tgttgggaat
     1861 tttgttgata ataaagtatt ttaaggttaa gcaatagaaa taataatctg cctgattata
     1921 gatgataaac tttttgtttt tatctggact gtgatattta tagttctaaa tggtttatta
     1981 tttataagtg ttgaaagaga ttgaagaaaa tggcttcgtg actcgctttc ttcggtcttt
     2041 ttcgttttat tatcaatttg taaacgattt acaatttgag tcaaattatg gtaaaatagt
     2101 gtcatgagta aaatgtatta tgcagaaaat cctgacgctg ctcacgacat tcatgagttg
     2161 agagtagagt tgttgggaca aaaaatgacc tttttaacgg atgcgggtgt ttttagcaag
     2221 aaaatagttg actttggaag tcaactattg ctcaagtgtc ttgaggtcaa ccaaggagaa
     2281 actgtccttg atgtaggctg tggttacgga cctttgggtt tgtccttagc taaggcttat
     2341 ggagttcagg ctactatggt tgatatcaac actcgtgcct tagatttagc tcggagaaat
     2401 gctgaaaaaa ataatgcaaa agcgacgatt ttccagtcca acatctatga acaagttgaa
     2461 ggacattttg atcatgtcat ttccaatcct cctatccgtg cgggcaagca agtcgttcat
     2521 gaaatcattg agaaaagtaa agatttcttg gaaactagtg gggatttgac aatcgtcatc
     2581 cagaaaaaac aaggggctcc aagtgctaag tccaagatgg agggtgtgtt tggtaattgt
     2641 gaaatcctta aaaaagacaa gggatactat atccttagaa gtgtgaaaga atgagagcag
     2701 ttgatttaat acaaaaaaaa cgagacggtc aagaactgac ttcaagtgaa attgaatggc
     2761 tggtagaagg ctatgtgtca ggaactgttc ctgattatca gatgtctgcc tttgctatgg
     2821 ctgtttattt taaaggaatg acgactcgag aaatctctga tttaacgatg aatatggtta
     2881 agactgggca agagtttgac ctgtcagcca ttgatggtgt gaaagttgac aagcattcta
     2941 ctggtggtgt cggcgataag gtaaccttga ttttagttcc tcttgttgct agcttcggtg
     3001 tgcctgtagc aaaaatgagt ggtcgtggtc ttggccatac aggcggaaca attgataaat
     3061 tggagtccat taagggctat caagtagaac gtagtcaaga ggattttatt cgtcaggttc
     3121 aggatattgg agtatctgtc attgggcaat cagaccagct ggtcaaagca gataagcttc
     3181 tctatgccct tcgtgatgtg accgcaactg tcgacacgat tcctttgatt gcgagttcgg
     3241 tgatgagcaa gaaaattgcg gcaggggcgg atgctatttt gctagacgtg actgtcggtg
     3301 agggtgcctt catgaagacg gttgatgagg cgcgtgagtt ggctcaaact atggtagatc
     3361 ttggtaaggt tgttggacgg aagacagtag cagtgattac ggatatgagc cagcctttgg
     3421 gacgagcgat tggaaatcgt ctggaaatcc ttgaagcatt ggagattttg caaggtcaag
     3481 gacgtcagga tattacccac tttatttgtg aattggctca aattatgctt ggtttggcaa
     3541 atgttaacaa aactgttgaa gaagttcgcc aacatcttga gaatggtcaa gcactggcta
     3601 agtttgagga aatggtgcaa gcccaaggtg gagacttgga agacctctat cgtcctgtaa
     3661 atgtagctca tgtggtggaa atccctgctc aggaaacggg tgtcatttca gctcttccag
     3721 ctatggactt tggtctttat gccatgaggc tgggtgctgg tcgtgcagtt aagtctgatg
     3781 ccttggacta tgaaacagga attgtttttg aaaagaagat tggagactcc gttcaaaagg
     3841 gtgaaattgt tgcaaaagta tacacaaatg aaaaaattcc acctcaacta gttacagatt
     3901 tccaaaaatg tgttaaaata agtgatgaag tgaaaaaaat ccgtgaaatt gtagaaatca
     3961 tctcctaaat ggagggaaaa ccgaaatgaa attaaataaa tatatagatc atacgctttt
     4021 aaaacaagat gcaaagaaaa aacaaattga tagtttgttg tctgaggcta gagagtatga
     4081 ctttgccagt gtttgcgtta atccgacctg ggttgaacat gctaaaaaag gacttgaagg
     4141 cacagatgtt aaggtttgca cagtagtagg tttccctttg ggagcaacaa cttcagccgt
     4201 gaaagcattt gagacaaaag aagctatcca aaatggtgca gatgagattg atatggtgat
     4261 caatgttgga gctctcaaat caggtaattt agccttggtt gagtcagata ttcgcgcagt
     4321 agtggaagca agtggtgata agttagtgaa agtcattatt gaagcttgcc ttctgacaga
     4381 ccaagaaaaa attgttgttt gccaattggc ccaaaaagct ggggctgact ttgttaaaac
     4441 atctactggt ttttcaactg gtggtgctac gatagcagat gttacattaa tgcgtgaaac
     4501 agttggatct gatatgggag tcaaggctgc tggtggagct cgttcttatg cagatgctct
     4561 tgcctttgtc gaagcaggtg cgacccgtat cggaacgtca gctggggtag ctattttaaa
     4621 aggagaattg gcagatggcg actactgagt tgattgaact ggcaattgaa accagcaaac
     4681 atgcatatgt cccctattct cactttccta tcggagcagt tttagtagcc aaagacggaa
     4741 gtgtttacac gggagtgaat atcgaaaatg ctagctatcc tttgaccaat tgcggtgaga
     4801 gaacagctat ttttaaggct atatctgaag gccaaagaga attttcagaa ttgattgtct
     4861 atggtcaaac tgaaaaaccg atctcgccat gtggtgcttg tcgccaagtg atggctgaat
     4921 tttttgaaca agatctaaaa gtgaccttag tcgcaaaaga taaatcgacg gtcgagatga
     4981 cggtcgggga attacttcca tactctttta cagacttaaa ctagtctgag tcgctctctg
     5041 agtggcacgg tccttgtggc caaccaatcc atacttgcaa catcgttgca catcttattt
     5101 aggaggttca gtaatgaaca agaaacaatg gctaggtctt ggcctagttg cagtggcagc
     5161 agttggactt gctgcatgtg gtaaccgctc ttctcgtaac gcagcttcat cttctgatgt
     5221 gaagacaaaa gcagcaatcg tcactgatac tggtggtgtt gatgacaaat cattcaacca
     5281 atcagcttgg gaaggtttgc aggcttgggg taaagaacac aatctttcaa aagataacgg
     5341 tttcacttac ttccaatcaa caagtgaagc tgactacgct aacaacttgc aacaagcggc
     5401 tggaagttac aacctaatct tcggtgttgg ttttgccctt cataatgcag ttaaagatgc
     5461 agcaaaagaa cacactgact tgaactatgt cttgattgat gatgtgatta aagaccaaaa
     5521 gaatgttgcg agcgtaactt tcgctgataa tgagtcaggt taccttgcag gtgtggctgc
     5581 agcaaaaaca actaagacaa aacaagttgg ttttgtaggt ggtatcgaat ctgaagttat
     5641 ctctcgtttt gaagcaggat tcaaggctgg tgttgcgtca gtagacccat ctatcaaagt
     5701 ccaagttgac tacgctggtt catttggtga tgcggctaaa ggtaaaacaa ttgcagccgc
     5761 acaatacgca gccggtgcag atattgttta ccaagtagct ggtggtacag gtgcaggtgt
     5821 ctttgcagag gcaaaatctc tcaacgaaag ccgtcctgaa aatgaaaaag tttgggttat
     5881 cggtgttgat cgtgaccaag aagcagaagg taaatacact tctaaagatg gcaaagaatc
     5941 aaactttgtt cttgtatcta ctttgaaaca agttggtaca actgtaaaag atatttctaa
     6001 caaggcagaa aaaggagaat tccctggcgg tcaagtgatc gtttactcat tgaaggataa
     6061 aggggttgac ttggcagtaa caaacctttc agaagaaggt aaaaaagctg tcgaagatgc
     6121 aaaagctaaa atccttgatg gaagcgtaaa agttcctgaa aaataatgga tggaagtcat
     6181 ttcttaggaa gcggccaagg gccgcttttt taagagttga gacaaagtca ttttgtctca
     6241 gtaaagcttg ctactagaca aactagcaag ttttattgaa ataaaataag actctgaaag
     6301 gaagagcaca tggcacacga aaatgtcatt gagatgcgtg atattaccaa ggtgtttggt
     6361 ggatttgttg ccaacgacaa aatcaacttg cacctacgaa aaggtgaaat tcatgcactt
     6421 ttaggagaaa atggggctgg taagtccacg ctaatgaaca tgttagcagg ccttcttgaa
     6481 ccaactagtg gtgaaatcgc ggtcaacggt caagttgtca atctcgactc cccatctaaa
     6541 gcagctagct tgggaatcgg gatggttcac cagcacttta tgttggttga agccttcaca
     6601 gtggctgaaa acatcatttt aggtagtgaa ttgactaaaa atggtgtgct agatatcgct
     6661 ggagctagca aagaaatcaa ggctctttct gaacgttatg gcttagctgt tgacccttct
     6721 gccaaggtag cagatatctc agttggagcc caacaacgtg tagaaatttt aaaaacactt
     6781 tatcgggggg ctgatatcct tatctttgac gaaccaacgg ctgttttgac tccatcagaa
     6841 attgatgagt tgatggctat tatgaaaaat cttgtcaaag aaggaaaatc aattatcttg
     6901 attacccaca aattggatga aattcgcgca gtttctgacc gtgttacagt tatccgtcgt
     6961 gggaaatcaa ttgaaaccgt tgaaattgca ggggctacca atgctgattt ggcggaaatg
     7021 atggtaggac gttctgtttc ctttaaaaca gagaagcaag cctctaaacc aaaagaagtg
     7081 gttttgtcta tcaaagattt ggtggtcaat gaaaaccgtg gtgttccagc tgttaaaaat
     7141 ctgtccttgg atgttcgtgc tggagagatt gttggtattg cggggattga tggaaatggt
     7201 cagtctgaac tgattcaagc cattacaggt cttcgtaagg ttgaatctgg tagcattgag
     7261 ctaaaagggg attcaattgt aggcttgcac ccacgtcaga ttacagaact aagtgttggg
     7321 cacgttccag aagaccgtca ccgtgatggc ttgattttgg aaatgatgat atctgaaaat
     7381 attgcccttc aaacctacta taaagaacca catagtaaaa atggaatttt gaattattca
     7441 aatattactt cttatgctaa aaagctgatg gaagagtttg atgttcgcgc tgccagtgaa
     7501 ttagttcctg cagctgcact ctcaggagga aatcaacaaa aagcaattat tgctcgtgaa
     7561 attgatcgag atcctgatct ccttatcgtt agccagccaa ctcgtgggtt ggatgtcggt
     7621 gccattgagt atatccacaa acgcttgatt gaagagcgtg ataatggcaa ggctgtcctt
     7681 gttgtcagct ttgaattgga tgagatttta aacgtctcag accgtattgc cgttatccac
     7741 gatggtaaga ttcaaggtat tgtatcacca gaaacaacca ataaacaaga acttggtgtc
     7801 ttgatggctg gtggaaactt gggaaaggag aagagtgatg tttaaaaaat tacaacaaat
     7861 ttcggttccc ttgatttctg tatttctagg aattttactc ggagccattg tcatgtggat
     7921 cttcggttat gatgctattt ggggctacga agaattgttc tatacagcct ttggcagtct
     7981 gcgtgggatt ggagaaatct tccgtgctat gggtcctctg gtcttgattg gtcttggttt
     8041 tgcggttgcc agtcgagctg gtttctttaa cgtcggactt cctggtcagg ctttggcagg
     8101 ttggattctc agtggttggt ttgccctgtc gcatccagat atgccccgtc ccttgatgat
     8161 tctagcaacc atcgtgattg ccttgattgc tggtgggatt gtcggagcga ttccaggtat
     8221 tcttagggcc tatctaggga cgtcagaggt tattgtaacc atcatgatga actacattgt
     8281 cttgtatgta gggaatgcct ttatccatgc tttccctaaa gacttcatgc aaagtacaga
     8341 ttcgaccatt cgtgttgggg ctaatgcaac ctatcagaca ccttggttgg ctgagttgac
     8401 tggtaactca cggatgaata ttggtatttt ctttgccatc attgccgttg cagttatttg
     8461 gttcatgctc aagaaaacaa ctcttggttt tgaaatccgt gcagttggtc ttaatccaca
     8521 tgcttcagaa tatgctggta tttctgccaa gcggactatt atcctatcta tgattatttc
     8581 aggtgccttg gcaggtcttg gtggagctgt tgaaggtttg ggaaccttcc agaacgtcta
     8641 tgttcaaggt tcgtcattag ctatcggatt taacggaatg gcggttagtt tgcttgcggc
     8701 caactcacca attggtatac tctttgcagc cttcctattt ggcgttctcc aagttggggc
     8761 tcctggtatg aatgctgcgc aggtaccatc tgagcttgtc agcattgtaa cagcgtctat
     8821 tatcttcttt gtcagtgttc attaccttat cgaacgcttt gttaaaccga aaaaacaagt
     8881 taaaggaggt aagtaaagat gtctattata accttgctcc ccctcttggt gtcttctatg
     8941 ctgatttact cagcacctct catctttaca agtatcggtg gtgttttctc tgaacgtggt
     9001 ggtgtggtaa acgttggcct tgaaggaatt atggttatgg gtgccttttc tggagttgtc
     9061 tttaaccttg aatttgcaga acaatttgga gcagcaactc catggctatc cttgcttgta
     9121 gcaggattgg ttggtagtgt tttctctatc atccacgcag cagcgacggt tcatttccgt
     9181 gcagaccatg ttgtcagcgg tacggtattg aacttgatgg cgcctgcctt ggctgttttc
     9241 ttagttaaag ttctttataa caaaggacaa accgacaacc taagtcaaac ttttggacgc
     9301 tttgatttcc cagtcttggc aaatatccca gtgattggtg atatcttctt caagtcaact
     9361 agtctacttg gttatctggc gattgccttc tcattccttg cttggtttat tctctttaag
     9421 actcaatttg gtcttcgtct ccgctctgtc ggtgaacatc ctcaagcagc ggataccttg
     9481 ggaatcaacg tctacaagat gagatattta gggattatta tttcaggttt tctaggtgga
     9541 attggcggag cgatttatgc gcaatcaatc tcagttaact tctcagtgac aactattgtt
     9601 ggacctggat ttatcgccct tgctgcgatg atctttggga aatggaatcc aatcggtgct
     9661 atgctttcta gtctcttctt tggactttca caaagtttgg ctgttatcgg ttctcaattg
     9721 ccgttcctac aaggagtgcc agcggtttat cttcaaattg caccttatgt tttgacaatt
     9781 cttgtcttag cagccttctt tggaaaagca gtcgcaccaa aagcagatgg tatcaactac
     9841 atcaaatcaa aataagcata caaaaaaacg tcagttcaat gactgaactg ccccccaaaa
     9901 gttagacaga aaaaatctaa cttttggggt g
//
LOCUS       DAVKMU010000058         9787 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKMU010000058 DAVKMU010000000
VERSION     DAVKMU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.058"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            262..357
                     /locus_tag="VW343_001808"
                     /pseudo
     CDS             262..357
                     /locus_tag="VW343_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(610..1230)
                     /locus_tag="VW343_001809"
     CDS             complement(610..1230)
                     /locus_tag="VW343_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9756321.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1230..1712)
                     /locus_tag="VW343_001810"
     CDS             complement(1230..1712)
                     /locus_tag="VW343_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9756322.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1712..2554)
                     /locus_tag="VW343_001811"
     CDS             complement(1712..2554)
                     /locus_tag="VW343_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9756323.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2649..3449)
                     /locus_tag="VW343_001812"
     CDS             complement(2649..3449)
                     /locus_tag="VW343_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9756324.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3593..4972
                     /gene="glmU"
                     /locus_tag="VW343_001813"
     CDS             3593..4972
                     /gene="glmU"
                     /locus_tag="VW343_001813"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9756325.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4982..5527
                     /locus_tag="VW343_001814"
     CDS             4982..5527
                     /locus_tag="VW343_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9756326.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5539..5853
                     /locus_tag="VW343_001815"
     CDS             5539..5853
                     /locus_tag="VW343_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9756327.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAAIFLLILLVLAMK
                     LL"
     gene            5870..6562
                     /locus_tag="VW343_001816"
     CDS             5870..6562
                     /locus_tag="VW343_001816"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9756328.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6639..7130
                     /gene="rocS"
                     /locus_tag="VW343_001817"
     CDS             6639..7130
                     /gene="rocS"
                     /locus_tag="VW343_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9756329.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7228..7818
                     /locus_tag="VW343_001818"
     CDS             7228..7818
                     /locus_tag="VW343_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9756330.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAD
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            7827..9590
                     /locus_tag="VW343_001819"
     CDS             7827..9590
                     /locus_tag="VW343_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9756331.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3023 a         1699 c         2086 g         2979 t
ORIGIN      
        1 agttagattt tttctgtcta acttttgggg tgtagttcat gtacacctga tatgatgcgt
       61 tttataattt taaagacttt ttgaccagcc tcattttttt aacttgatac tcagtgaaaa
      121 gcaaagatta aactaggaag ctagctgtag gctgctcaaa gaacagcttt gaggttgcag
      181 ataaaacttg tgaggtcacc aacatatata atgtgaagct gacgtggctt gaatagattt
      241 tagaagggta tgagtctgga agttttaatg gataatgcaa gattccatag aatgggtaag
      301 ctagagttct tatgtgaaga gtttgggcat aaacttttac cttttcctcc ctactcatct
      361 tagtatagaa aagtgaatct aaaatagtac ataactgctt ctaaaacatt cttataaatt
      421 gatttaaatt ctcaaatcat attattcagt tcttatttca ttttgctcta caatcctgtt
      481 gagaagacac gtgttcatat caaaaaggta ttggcaagtt gcaatacctt tttacaaggt
      541 tcttttgtct tatttttgtt tcaactgact atatctccta tggttctagt tcagaaggct
      601 aggctataat tatgattgat aagaagtatc attccaagta ttgagagtga atgtttcaaa
      661 atcatgggtt tctataatgg tcaggctggc atttgctaga ccgccatctt tacgaagaag
      721 tggttcttta tagcctagga gagtacgaag actggcagta agattggcgc cgtgtccgac
      781 aattagaata cgctcagctg gactatcttt taatgatttg ataaattgga tggtccgttg
      841 agttgtacta tagagggatt cggctccgaa cattcgagtg tcaaattgag caagatttga
      901 acgaaaagcc tggatttgtt gcgggtaaat agcttccaag gttgcaattt tcaaaccttc
      961 taacttccca agttgccatt cacggagatt aggaacgatt tctaaagaac agggggtata
     1021 gagttgactt tggataatct cagcagattt gaccgctcga ggtaaatcac ttgaataaat
     1081 ctgatcaaaa ggaatttcct tgagatactg gccaagtcgt tttagggttt caatggattc
     1141 aggaagaaga ggagaatcac cgctagcacc ttgaaaacga ccttcttggt tccagagggt
     1201 acgaccgtgg cggacaaagt agagtttcat tacttgatgt cctccaaaat atctacaaag
     1261 tctgccttta caaagctagc caagtcttgt ggcgcgacga taatgctgtg tccgacttcg
     1321 cctgcagaga caatcattcg atccaaatct agagcaattt tatcgataaa aatgggataa
     1381 ttgtgtttct gacgaattcc gacaggatta ttggctccat gaatgtaacc agttgttttt
     1441 tctaagtcct tttgtggaat catgctcact tttttattgc cagaaatttt agctagtttc
     1501 ttttcagaca agtgctgagt gatagggaca attccgataa tcggtccggt cttgtctccc
     1561 aaaagcgcca aggttttgaa aatctgatct cgttcataac cttgaggaag ctctccttct
     1621 agggcattga tttgaatccc ctgatgaggg atagctgctt tagataggat ttgttccacc
     1681 aatgtttttt tgattttaac tttttttgcc attatttata tttatcctcc aattgactca
     1741 tccaaatacc aagccagatt cccagcgcaa agaagaaggc gatgatgaca taaccgacaa
     1801 gtgaaagtcc tgtgtattgg atactttcag cgtttcctgc atttggaatt aagatcaaaa
     1861 gggtacttga taggacgata ccgatgatga aatgatagac gcgtgagtgg tagttgttta
     1921 aggcataatc catcaatttt gaaaaaacga tgagagttgc acctgcacca attccaatcg
     1981 gaaagaaggt tcccaagaga tcaaaagttt taaaaccagt caacatagga gcatagagtc
     2041 ccaaaatcaa aagtaagttc gatgggctga ggccaggaac caagacgcca agggccaata
     2101 gtgcgcctgc taggacgaag ttaagaaagc tggcgcttaa ggttccaacg acaaaattta
     2161 aggcatagag tcctaatcca gaaatgataa aggttgtcca taaccaagct aaatcaatct
     2221 tgtctcggtc agattctcga gttgattctt tgaggaggct aggaactgta ccgataatag
     2281 ctcccgcaaa gctccataat acaaagacct gataattttc aagcaggtat tcaatcgggt
     2341 aggaaaataa gccgattccc agaagcatac cgatggcaac tggaataaag tacaaaacat
     2401 tttctttaag gtctttaaag ggatgggcca gaaagccaat cattcgttca tagattccta
     2461 agattgctgc tagaacccct ccggaaattc ccggtaggat aaatccaaga gcaattacaa
     2521 tccctttaat aacgcgtgct aaccatgaga gcatatattt cctccaacta tttctatttc
     2581 aaaaaatcct tactatattg tatcacaatc agacacaaaa agaaaaagca aatgataaac
     2641 aaatgctttt aaggttttaa aaaaagtttt cgaaaggttt cttctttatt ttttaaggga
     2701 gagataacgt tgatatctaa atcgtggtca aagccggcaa tttttccttt agatgtgtat
     2761 tggtgaatat cataatctaa atcagtttta ggactgctct ccaaaaatcc tgagtctgag
     2821 ccgtaggacg gaatccaaac agaggtaaac ttgcctgtat caatactgtg ttcttccatg
     2881 aagtagacac caacgtagat gccgatgttt ttagcaccta gtgatgctag ttttgctcga
     2941 aagttttcga caccttcgtt catattagac atggttttgt cttccacgtc aagccaatag
     3001 taactagggc tgtaaggaga ggcagcattg tagaaaactt cggcagcctt ttccatttct
     3061 tggacacttt ttccagctac ataagcgtag acagcaactg ggacattccg cttttgaagt
     3121 tcagtgatat gactcttata ggccttgtct attccattga taaatgaagc atcattttct
     3181 tttgttgttt gagcaccact gtgaacacga acaatagcac ctgaaatatt ttgtgagagg
     3241 gcatcgtagt tgatttcctc aggacgctgc cagccagaga ggtcaataat cggtttgtct
     3301 aagtgtttca aagcctgtgc ttcaatctgt gctatattgg attttgtttt aaacgattgg
     3361 ctgtcattaa gtgggcgatt gatgattaaa atgaacatca taatcccaaa aaaactaaat
     3421 aaaataagtg gatgaatttg ttttctcata tcttataagt ctaccctaaa aatcaaaaaa
     3481 aatcaaaaaa atgggttaag gaagagactt tagagcattt tttcattcaa gagtgcggaa
     3541 tgatttgaaa tatggtataa taaaagggaa tttctacaga aaagagaaga ttatgtcaaa
     3601 ttttgccatt attttagcag cgggtaaagg gactcgcatg aaatctgatt tgccaaaagt
     3661 tttgcacaag gttgcgggta tttctatgtt ggaacatgtt ttccgtagtg tgggagctat
     3721 ccaacctgaa aagacagtaa cagttgtagg acacaaggca gaattggttg aggaggtctt
     3781 ggctggacag acagaatttg tgactcaatc tgaacagttg ggaactggtc atgcagttat
     3841 gatgacagaa cctatcttag aaggtttgtc aggacacacc ttggtcattg caggagatac
     3901 tcctttaatc actggtgaaa gcttgaaaaa cttgattgat ttccatatca atcataaaaa
     3961 tgtggccact atcttgactg ctgaaacgga taatcctttt ggctatggac gaattgttcg
     4021 taatgacaat gctgaggttc ttcgtattgt tgagcagaag gatgctacag attttgaaaa
     4081 gcaaatcaag gaaatcaaca ctggaacata cgtctttgac aacgagcgtt tgtttgaggc
     4141 tttgaaaaat atcaatacca ataacgctca aggcgaatac tatattacag acgtcattgg
     4201 tattttccgt gaaactggtg aaaaagttgg cgcttatact ttgaaagatt ttgatgaaag
     4261 tcttggggta aatgaccgtg tggcgcttgc gacagctgag tcagttatgc gtcgtcgcat
     4321 caatcataaa cacatggtca acggtgttag ctttgtcaat ccagaagcaa cttatatcga
     4381 tattgatgtt gagattgctc cggaagttca aatcgaagcc aatgttacct tgaaagggca
     4441 aacgaaaatt ggtgctgaga ctgttttgac aaacggtact tatgtagtgg acagcactat
     4501 cggagcagga gcggtcatta ccaattctat gattgaggaa agtagtgttg cagacggtgt
     4561 gacagtcggt ccttatgctc acattcgtcc aaattcaagt ctgggtgccc aagttcatat
     4621 tggtaacttt gttgaggtga aaggatcttc aatcggtgag aataccaagg ctggtcattt
     4681 gacttatatc ggaaactgtg aagtgggaag caacgttaat ttcggtgctg gaactattac
     4741 agtcaactat gacggcaaaa acaaatacaa gacagtcatt ggagacaatg tctttgttgg
     4801 ttcaaattca accattattg caccagtaga acttggtgac aattccctcg ttggtgctgg
     4861 ttcaactatt actaaagacg tgccagcaga tgctattgct attggtcgcg gtcgtcagat
     4921 caataaagac gaatatgcaa cacgtcttcc tcatcatcct aagaaccagt aggagcctat
     4981 catggagttt gaagaaaaaa cgcttagccg aaaagaaatc tatcaaggac caatatttaa
     5041 actggtccaa gatcaggttg aattaccaga aggcaaggga actgcccgac gggatttgat
     5101 tttccacaat ggggctgtct gtgttttagc agtaacggat gaacaaaaac ttatcttggt
     5161 caagcagtac cgcaaagcta tcgaggctgt ctcttacgaa attccagccg gaaaattgga
     5221 agtaggagaa aacacagccc ctgtggcagc tgcccttcgt gaattagagg aagaaacagc
     5281 ctatacaggg aaattagaac tcttgtacga tttttattca gctattggct tttgtaatga
     5341 gaagttaaaa ctatatttag caagcgattt gacaaaagtg gaaaatccgc gtccgcagga
     5401 tgaggatgaa accttggaag tccttgaagt gagcttagaa gaagcgaaag aattaatcca
     5461 atcaggtcat atctgtgatg ccaagacaat tatggctgtt cagtattggg agttgcagaa
     5521 aaaatagagg aggtcagtat gggtaaatct ttattaacgg atgaaatgat tgaaagagct
     5581 aatagaggcg aaaaaatttc aggtcctcct ttgctagatg ataatgagga aactaagatt
     5641 ttaccaacct cttcttcccg ttttggttat gccaatccta aggatcatgg ttttagccag
     5701 gaaaccttga agattcaggt cgaaccatct attcataaaa gccgtcgtat tgaaaatacc
     5761 aagagaaatg tcttcaattc taagttgaat aaaatcttat ttgcggccat ctttctcttg
     5821 attttgcttg ttttagcaat gaaacttttg taatagaaaa ggaattgaaa tgaaaatagg
     5881 aattattgct gctatgccag aagaactggc ttatctggtc cagcatttag ataataccca
     5941 ggagcaagtt gttttgggga atacctatca tacaggaacc attgcttctc atgaagtcgt
     6001 tcttgtagaa agtggaattg gtaaggtcat gtctgctatg agtgtggcga ttttggctga
     6061 tcatttccag gtggatgccc ttattaatac gggttcagct ggggcagtag cagaaggtat
     6121 cgctgttggg gatgtcgtga ttgctgacaa attagcctat catgacgtgg atgtcacagc
     6181 ttttggctat gcttatggac aaatggcgca acaaccgctt tatttcgaat cagacaaaac
     6241 ctttgttgct caaatccaag agagtttatc tcaattggac caaaactggc atcttggttt
     6301 gattgctaca ggagatagtt ttgttgcagg aaatgacaag atagaagcga ttaagtccca
     6361 tttcccagaa gttttagccg tggagatgga gggggcagct attgctcaag cagcgcatgc
     6421 cctcaatctc ccagtcttag tcatcagagc tatgagtgac aatgccaacc atgaagcaaa
     6481 catctttttt gatgagttta ttatcgaagc tggacgtcgc tctgcccaag tcttgttgac
     6541 ctttttgaag gctttagatt aagcggaaat ttgacagttt ttctagctta tgataagatt
     6601 taagtaaaga aaagctagaa aacgtttcag aggatattat gagtattgaa atgaccgtca
     6661 gtgagattgc agaggtctta ggattatctc gccaagcaat caataaccgt gtcaaagaat
     6721 taccagaaga agacacagat aaaaatgaca aaggggtaac agttgttacc agaagtggct
     6781 tgattaagct agaagaaatc tataaaaaaa cgatttttga agatgagcct gtcagtgaag
     6841 atgtcaaaca acgtgaactg atggagattc tagtggatga gaagaatgca gaaattcttc
     6901 gtctttatga acaattaaaa gccaaggatc gtcagttatc agaaaaagac gagcagatgc
     6961 gtatcaaaga ccgtcagatt gctgagaaag acaaacaatt agatcagcaa caacaattga
     7021 cccttcaagc catgaaggat caagaaaatc ttaagctaga gctggaccaa gcaaaagaag
     7081 aagtccaatc cactaagaaa ggcttttttg ctcgtttatt tggaggataa tcaaggagct
     7141 gtttaggtta aatgctaacc tgatggcttc ttttgtttgt gaatagtata gttgattaga
     7201 acagagacag tagaggagag gtacagaatg gaacgattag aagattacat tgcttttgac
     7261 ttagaattta atcagcacga aggacaaaca cacttgattc aagtatcggc agtgcgtttt
     7321 agagatggtc gggaaatcga tgcctatgat tcctatgttc ataccagcgt ccctctaaaa
     7381 agctttatca atggtttgac cgggattaca gctgagacct taaaagatgc tcctccagtt
     7441 gagaaagtct tagaagaatt ccaagagttt gtgggttctt tgctgatgat aggctacaat
     7501 gctgctaaaa gtgatttacc tattttagca gatcatggtt tagactatag ccaacagtac
     7561 aaggtggatt tgtatgatga agcttttgaa cgtcggagtt cagatttaca tggtattgcc
     7621 aatctcaaat tgcaaactgt tgcctcattt cttggatttc aaggtcagtc tcataatagt
     7681 ttagaggatg ctcggatgac ggcgcgtgtt tacgaatcct tcttagagtc ggatcaagct
     7741 agggaattgt tagagacaga aagtagctta tcaaacaatc cttttggtgt cttggacttg
     7801 tctcaattat ttgactagga gtgcctatga aacctgaaaa acctaaaaaa ttgggtctta
     7861 aacagattta cctcaatctt gataaaatcc tctttctatt tttcttgatt tttatgatag
     7921 tggagtattt gtgggtacca ttcaattcat ggttggctag cttcttatta gatcagacag
     7981 gttatctttt tatttcctat aacaatgtct tagccatcct tactcattct ccattgatta
     8041 gtctagcttt tttagccttg attgtaatta accttctggt tgcttatttt caaataggac
     8101 tcctctttat tggtgctcgt catctcctct ataatgaaaa gcgaaggcta attgagtata
     8161 tccgcaaggt tttccatgaa agttttggat ttatgaaaaa gctaacttgc gctaaagcct
     8221 tgtttatctt tttctatata gctatgcttt ttccttttat acggaaaatg ctgaagattt
     8281 attatcttaa taaaatcgtc attccagagt ttatccaagc ttatttagaa gataaatatt
     8341 ggatgtggtg gctgagcatc ctacttctat ctcttatctt tctctatgtc tctgtcagat
     8401 tgatgtttgc ccttccgaag attgtatatg atcagctgac tgttcgagaa gcagtgatgt
     8461 ttagcttgga gaaaaccaag aagagagtta ctttttatgc ttggaattta ttttacatct
     8521 tgctcaaagc acatttatta ttttatcttc ctttgattcc tttattattg gctcaaactt
     8581 tggtagatga tatcactcag agagagtctc tgattcttgg tattttgaat tttgtcgtga
     8641 ttaaaaatct ctattatatg gctctgactt attttttggt taaatttgtt tcatttttga
     8701 caggaaagga gctggatatg cttcctagga gagaaaaaga tcacatcgtg cgatggggtg
     8761 tcatgacttg tgccagtctt ttctttgcct tagaaggtta tatttatctg gaggctccca
     8821 tggttcatct acctcaactt atttctcacc gaggggtttc caatgcaaat gggattcaaa
     8881 atacagtaga gtccttggaa actacagcac aactcaaacc agacttggtg gagacggacg
     8941 tgcaggaaac aaaagatggg cagtttgtca tgatgcatga tgctaacttg aaaaatctag
     9001 caggtatcaa taaaagtcct caagacttaa acttggagga gcttaaaggg attgatattt
     9061 ttgaaaatgg ctaccagact aaaatttcaa gctttgaaga ttatctcagt cgagccaacg
     9121 aacttggtca aaaattacta attgaaatta aaaccagtaa aaaagatagt ccagacatga
     9181 tgaaccgctt tttagcccgt tatgctgcaa agctcaagat ttatggacat caaatccagt
     9241 ctttagacta ccatgttgtc gaaaaagtaa gacagtatga tgccgaactg ccagtttatt
     9301 tcatcatgcc ctacaattct gtctttccta aaacaagagc gacaggatat actatggagt
     9361 actcaacctt ggatgaatat tttgtaagca aactatggac aacggatcag aaactttatg
     9421 tctggactgt caatgattca gaagctatca gcaaatctct tcacttagga gtagatggag
     9481 tgattacaga tgatttggaa aaagttcagc gggaaataga agtagcccaa gaagatccag
     9541 aatatacgga tttgctcttg aaaaaagcat tggaattctt tgaattttag taataaaaaa
     9601 gaggaacggt tgttcctccc atgataggta agtattcttg tgaatgcttg cttttttgtg
     9661 gttgcaatcc gtttacgaga attgtttgta ttgttcttgt ttcaatttac tttatagtgc
     9721 tttgaagttg gaatgctaca ctataacttc taaaatatag taaaatgaac caaaaatagt
     9781 acacaat
//
LOCUS       DAVKMU010000059         8956 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKMU010000059 DAVKMU010000000
VERSION     DAVKMU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.059"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(298..1146)
                     /gene="dprA"
                     /locus_tag="VW343_001820"
     CDS             complement(298..1146)
                     /gene="dprA"
                     /locus_tag="VW343_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9756332.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            complement(1242..1931)
                     /locus_tag="VW343_001821"
     CDS             complement(1242..1931)
                     /locus_tag="VW343_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9756333.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1943..2821)
                     /locus_tag="VW343_001822"
     CDS             complement(1943..2821)
                     /locus_tag="VW343_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9756334.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2811..3680)
                     /gene="licA"
                     /locus_tag="VW343_001823"
     CDS             complement(2811..3680)
                     /gene="licA"
                     /locus_tag="VW343_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9756335.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3697..4719)
                     /locus_tag="VW343_001824"
     CDS             complement(3697..4719)
                     /locus_tag="VW343_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9756336.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4724..5431)
                     /locus_tag="VW343_001825"
     CDS             complement(4724..5431)
                     /locus_tag="VW343_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9756337.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5767..7254
                     /gene="tacF"
                     /locus_tag="VW343_001826"
     CDS             5767..7254
                     /gene="tacF"
                     /locus_tag="VW343_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9756338.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVVPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7264..8067
                     /locus_tag="VW343_001827"
     CDS             7264..8067
                     /locus_tag="VW343_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9756339.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8069..8878
                     /locus_tag="VW343_001828"
     CDS             8069..8878
                     /locus_tag="VW343_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9756340.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2811 a         1804 c         1518 g         2823 t
ORIGIN      
        1 aaagcgcaaa ctaggaagct agccacaggt tgctcaaagc actgctttga ggttgtagat
       61 aagactgacg aactcagctc aaaatactgt tttgaggttg cagatggaag ctgacgtagt
      121 ttgaagagat tttcgaagag tattatatga gaaaaatcct ctactctact aatccactta
      181 gaaaaaacgc atattagcaa gtgttaaaaa cttgctaata tgcgttttat tatggaaatg
      241 ttgggttcat tttctcttaa aattgatttt ttcttagaat tctagcttag gtcattttta
      301 aaattcaaat tccgcaagaa catcttgccc actggtgagc aattttgctc cttcttgaat
      361 caaatgatgg caaccgtctg atagtccatc taaaatgcta ccaggaatag caaagacatc
      421 gcgtccttct tccattgctc gctcacacgt aatgagacta cctgaacgca tcttagcctc
      481 tgctacaatc acaccacgac aaagtccagc aatgatgcga ttacgggcag gaaaatgaaa
      541 tttcagaggt tgttcgccag gtccatattc acttagaacc agatggtcat tgccgatgta
      601 gtcttgcaag cgtttattgg ctttaggata aaacacatcc agtcctgttc caatcactgc
      661 aatggttttt ccgccattct gaagagctgc catatgagct gctgtgtcaa tgcccttggc
      721 cagaccactg acaataacca gttcattttc caagccttga atgacttttt caactgactt
      781 agctccctgt ttgctacaag cacgactgcc cacgaccgct accttcggga atttcaggag
      841 gtcaagattt cccttgtaaa ataaaagtac aggcgcatca tatatttcac tcaaatccca
      901 aggataacag tcatctaaaa tagagaaaga tggaaatttt tgaaactctt tcgacaaatg
      961 cgcatcgtct atctgaaaat aacgttccat aaaaacggct ggattacggc aacctgatat
     1021 atctgcaata tcacccaaca aaagctcctg atcaacattt tcaccgtatt ctagcacttt
     1081 caaaatctgt tgattggtca aacctgattt ttttaactta tagatttcat agtttgtgat
     1141 tttcataaat aactccattt ctttttctac tcatctatct attcgtaaaa gttcgtaaaa
     1201 aatcaaaaaa catccgacta ttttgtcaga tgttggaatc tttaattttc gtttttaaga
     1261 atttcttcta atttacgata gtcttggaca ctatcgatct cataaatgct attgccttct
     1321 aattcttcaa catagacatc tagctctttg atattatcct taaccatatt gtcccaatag
     1381 agatcaacaa attcaccact tgcataagcc ttgtcgataa agctgacaat cttttctgca
     1441 gttggagcat cccagaagga tacaccacta aggatgcgac ctgccttgct atcaacaata
     1501 atgtcttgaa ccttgtagtc atctccatag accaagaacc attcgttggt acaatcttca
     1561 cgataaacac taaaataagt cgaacgtgtc aaatcattgc ggaacatatt tttaaagaga
     1621 taattgtcag catcaataac atagctgttg gccaattctt cttttacaag atagagagag
     1681 taaaagttat tgtagtcagc gtatttatca ttgaaaacga ggcgaacacc gtatttttct
     1741 tttaagtaat cgaattgttc tttaagataa ccaacaatga tgatgatgtc attgattcct
     1801 ttttctttga gaaactcaat ttggtattca atcaaaggtt tttgattaac ctgaaccaag
     1861 gctttagggg tattttcagt cataggacgc aagcgagttc ccaatcccgc tgctaagata
     1921 atggctttca cacgaatctc ctttattctt taataataat ataaactcca gcaatgacga
     1981 caagtgacgt cataatggtc agcatatcta gcggtgcacc caaaaaaaca actgcaaaca
     2041 agaccgtcca tactacatag ctcacgttca agcctgtagc cttggctggt tgcaagcgat
     2101 tgatagcgat ataataagcc aagtaggaaa tcatatcaaa ggctgcaaaa acaatcatga
     2161 gacctagcaa ttgtccattg gctactgcag taaatgactg atgagagaag agcacaatca
     2221 caagatagga caagaacgaa gtcacttgac ggattaagag ggcttcgatt tcactcagtt
     2281 cactttccat agcaaaggag ctaagaacac tctcacttcc ccatgcaata gcacaaacca
     2341 aagcacagag aatcccaatg tagaaggaat taacctgttc aaccttatag gtctgggcaa
     2401 taatccctcc aataatcaag acaatcccaa atacagtatt tttcgaaatc ttgtgcttca
     2461 aaaagaagaa agccaataga actgaaatcg cagggtaaat agccgataca gatgaagcta
     2521 aagaacttcc gatatactta actgcataaa gattggcctg cataccgata gggcctgcta
     2581 gcaaggctcc gatgataaca ctgacattgc gaatatttaa gaaaattgag aggcgaactt
     2641 tcccttcttt taccaagaga aaagctagta agataaagat gctcaaaaaa tcatgagctg
     2701 cagccaccac aaagggtgac aaatctgtaa aaatcgaaaa gatataagca ctaaccgtta
     2761 gacccaagcc ccagaaaata cctgagagaa ggccaaaagg aactccgttt ttacttttca
     2821 tctgaacctc cataagaagc caaaccttta acagctcttt ggtaacgatt cacaccatag
     2881 tcaccaaaat cttcaccttg ctcttcctta tagacagtcc atagactcca aatagtatct
     2941 tgtaaaattt tataaatagc aatcttttca tgagaaaccg gtgtttggtc actctcatag
     3001 tgagataaga aagtttcttc ctcttgggaa gtgaattcag actctaaaaa gagggcagcc
     3061 aaatcccaca ttggatcatt cattgatgaa tattcccagt caatcaaata aagtcgtcct
     3121 tgaggtgatt cgataaagtt ttcaggcacc aaatcgatat gacaagattt tctgtcaaca
     3181 cctaagtcag ccagtctttt ctctaaggag aagactgcat ttctaacaga ttcatagttg
     3241 gcataaggaa tttgttcttc aatcaaggat tcgtattttt tgatttcttc aaaaggagca
     3301 aattctcctc ttaattcctt agcagacgta tgaatagttt gtaatattgg agcaattttg
     3361 tcgaacttgg tcttgattga cgttgaatca agcgtaatcg cagattcgat atactcattt
     3421 actttgatac cagcttcaat atcaaaaaga taatttttta catctaagcc taaatccttt
     3481 agtagttcaa gattgtactt ttcatcttgt cgattgataa gcttttctgt ccctttacca
     3541 aagaatttaa caatgtattg cttatttgtt gttttggcca aatagttttg attggtcatt
     3601 ccacccagtt gttcaacact gaggacttcc tcttcttgac taagtaagga agaaattttt
     3661 tctttaatga ttttctccac aattaacctc cagtacttat acttcccact taaacactgt
     3721 tttaaaggct gtgtttaaat cggttgcaaa gacacgatga atatctttaa tttctcttac
     3781 aggttcttct agataaagga tatttttaag acgattggca aatttcttga cttccatcat
     3841 ttggatagca tttttaaaat caatgcgacc agaacgagat gacccaacca aaatcaagcc
     3901 cttttctaag gcatcgcgag tattgagatt gactttatat tcgctaactc ccatcatgag
     3961 aatcgttccc tgaggacgaa tgtagcgaat caagtcatta atagctggtc cagtaccatc
     4021 accaccacaa cattcaaaag catggtcaaa ggccaactct tcaggaatat tatccgtaat
     4081 atagcattct ttggcaaatg agaagagttc caacttttcc caatgacgac caataaccac
     4141 aatctctgat tctggcaaag tatagttgat aatattggca accacaaaag ctaaacttcc
     4201 atctccaata acggcgatcc gctcccgctt gctatgagca agagtcaata gacgattcat
     4261 agcgtgcatg cccacactga caaactctgt aatggctgca accgtatctt caatagcatc
     4321 ataagccacc acacgatctt tagggagaga aacaaactct ctcataaagc catcaaatcc
     4381 actagacaag aaatgggtcc ctgtcatgta gttttcatag aattcttcat cactctgcat
     4441 aggagactga ttgggaatca tgacaacttt ttgaccaacc tcgtaggttc cggtcgggtc
     4501 agaaatgacg gttccacatg actcgtgaat cattgccatt ggaagctttt tattcaaaat
     4561 cttgggatca cgttttccct gatagtaacg ctgatccgca tgacagacag ccatgtagtt
     4621 gggacggata aggatatgat tctcttggtc aatagcctct tcctgatatt tgacattgat
     4681 aaacttaggc ttagttagtt gataaatttg attaatcatt ttactagtct ttctcaatca
     4741 tactttttgc aatcttcaaa tctgttacgg ttgtaatctt cagatttgag tattcacctt
     4801 tggccaaggc cacatctttt cctttgatca caaagatttt acatgcatct gtcaagattt
     4861 ccttctcttc atcagaaaga gatccataaa ggtccatgaa gtccttgcaa cggaatgttt
     4921 gaggtgtttg tccttgataa aggtgagcac gatttggaat atctgtaata aattgaccat
     4981 tggtactttc aacgatagta tcaaccgctt ctaccactgt gtccactgcg tcatgatttt
     5041 gggcaagttg gatattgtcc tgaatcatgc gaagtgtaat aaatggacga acagaatcgt
     5101 gggtaacaac gatatcctct ggagtaagcg gacgataagc atcaatggct tcaatgattt
     5161 tcttaatact tgtattgcgg tcagcaccac cctttgtaat gatgatacgt tccttataaa
     5221 gaggaagata tttatctaca agatcttctg catgagaaac ccagtctcca tgaacaccaa
     5281 ctacaatttt ttcaatactt ggctccaaga caaatttttc aattgtatga atcaaaatag
     5341 gtcgattacc tagctctaaa aattgttttg gcaagttact gatccccatg cgtgtgccag
     5401 ttccaccggc aagaattcct gcataaatca tctattttct cctttgtctt actaaaaaaa
     5461 cttattctct ctattatacc acaatctagt cctatcataa aagaaatact ggtcctcccc
     5521 ttctagaata ggagaattat gcagttctat tattcaaaga aactagtcca tttctagtaa
     5581 tgactaggaa ttctgtagca attactaata aatggctgcg aaattttaga gtccttcaga
     5641 ataatatact ttccttaaag aaaacttaaa acttaagatt tgaaacttca aagtgattac
     5701 ttgacttact gcccttcatt tcttattagc taactttatg atataatgaa aaacgaggta
     5761 aattgaatga aaagtataaa attaaatgct ctatcttaca tgggaattcg tgtcttgaat
     5821 attatttttc ccatcctaac tggaacctat gtcgcgcgtg tcttggaccg aactgactat
     5881 ggttacttca actcagtcga cactattttg tcatttttct tgccctttgc aacttatggt
     5941 gtctataact acggtttaag ggctatcagt aatgtcaagg ataacaaaaa agatcttaac
     6001 agaacctttt ctagtctttt ttatttgtgc atcgcttgta cgattttgac cactgctgtc
     6061 tatatcctag cctatcctct cttctttatt gataatccaa tcgtcaaaaa ggtctacctt
     6121 gttatgggga ttcaactcat tgcccagatt ttttcaatcg aatgggtcaa tgaagctctg
     6181 gaaaattaca gttttctctt ttacaaaact gccttcatcc gtatcctgat gctggtctct
     6241 attttcttat ttgttaaaaa tgaacacgat attgttgtct atacacttgt gatgagttta
     6301 tcgacgctga ttaactacct gattagttat ttttggatta aaagagacat caaacttgtt
     6361 aagattcacc taagtgattt taaaccactc tttctccctc tgacagccat gttagtcttt
     6421 gccaatgcca atatgctctt cactttttta gatcgcctct tcctcgttaa aacagggatt
     6481 gatgtcaacg ttagttacta taccatagct cagcgaattg tgaccgttat agctggggtt
     6541 gtaacaggtg caattggagt gagtgtgcct cgtctcagtt actatctggg gaaaggagac
     6601 aaagaagcct atgtttctct ggttaataga ggtagtcgaa tctttaactt ctttatcatt
     6661 ccactgagtt ttggactcat ggttttagga ccaaatgcca tcctacttta cggtagtgaa
     6721 aaatatatcg gaggcggcat cttgacctct ctcttcgctt ttcgtacgat tatcctggcc
     6781 ttagatacca ttcttggttc ccaaattctc tttaccaatg gctatgaaaa acgtatcaca
     6841 gtctatacag tctttgctgg gctactcaat ttgggcttga atagtctcct ctttttcaac
     6901 catatcgtgg ttcctgaata ctacttactg acaactatgt tatcagagac ttctctactt
     6961 gttttctata ttattttcat ccatagaaaa caactcatcc acttgggaca tatctttagc
     7021 tatactgttc gatactctct cttttcactt tcctttgtag caatttattt cctgattaat
     7081 ttcgtgtatc ctgtggatat ggtcattaat ttgccatttt taattaatac tggtttgatt
     7141 gtcttgctat cagctatctc ttatattagt ctacttgtct tcacaaaaga tagcattttc
     7201 tatgaatttt taaaccatgt cctagcctta aaaaataaat tcaaaaaatc ataggagttt
     7261 aaaatgaaac aactaaccat tgaagatgcc aaacaaattg aattagaaat tttggattat
     7321 attgatactc tctgtaaaaa gcacaatatc aactatatta ttaactacgg tactctgatt
     7381 ggggcggttc gacatgaggg ctttatccct tgggacgacg atattgattt atccatgcct
     7441 agagaagact accaacgatt tattaacatt tttcaaaagg aaaaaagcaa gtataagctc
     7501 ctatccttag aaactgataa gaactacttt aacaacttta tcaagataac cgacagtacg
     7561 actaaaatta ttgatactcg aaatacaaaa acctatgagt ctggtatctt tatcgatatt
     7621 ttccctatag atcgctttga tgatcctaag gtcattgata cttgttataa actggaaagc
     7681 ttcaaactgc tgtctttcag taaacataaa aatattgtct ataaggatag ccttttaaaa
     7741 gattggatac gaacagcctt ttggttgctc cttcgaccgg tttctcctcg ttattttgca
     7801 aataaaatcg agaaagaaat tcaaaaatat agtcgtgaaa atgggcagta tatggctttt
     7861 attccttcaa aatttaagga aaaggaagtc ttcccaagtg gtacctttga taaaacaatc
     7921 gatttaccct ttgagaattt aagccttcct gcacctgaaa aatttgatac tattttgaca
     7981 caattttatg gagattatat gaccctacca ccagaagaaa aacgcttcta cagtcatgaa
     8041 tttcacgctt ataaattgga ggattaggat gcaatattta gaaaaaaaag aaattaaaga
     8101 aattcaacta gccctgctgg actatattga tgagacttgt aagaaacatg atattcctta
     8161 ttttctcagt tatggaacca tgcttggagc catccgccac aaaggtatga ttccttggga
     8221 tgatgatatt gatatttccc tttatcgtga ggattatgag cgtttactga agattattga
     8281 agaagaaaat caccctcgct acaaggttct ttcctacgat acatcttctt ggtacttcca
     8341 taatttcgca tcgattttgg acacttctac tgttatagaa gaccatgtta agtacaagcg
     8401 tcatgatacc agccttttca tcgatgtctt cccaattgat cgatttacag acttgagcat
     8461 tgtcgacaag agctataagt atgtggccct tcgtcaacta gcttatatca aaaaatcacg
     8521 agcagttcac ggtgatagca aactaaaaga ttttcttaga ttatgtagct ggtacgctct
     8581 ccgatttgtc aatcctcgct acttttacaa gaaaattgat caactagtca aaaatgctgt
     8641 aaccaacact cctcaatatg aaggaggagt tgggatcggt aaggaaggga tgaaagaaat
     8701 cttcccagtt gataccttta aagaactgat tttaactgag tttgagggcc gtatgttgcc
     8761 tgttcccaaa aaatatgacc aatttttaac ccagatgtat ggcgattata tgacaccacc
     8821 atcaaaagaa atgcaagagt ggtatagtca tagcattaaa gcttatcgca aaaactgatt
     8881 gagggggatt atacaaacta ctaagataga ggttattcaa aaacataatt ttagtagaaa
     8941 atgaaatata tattcc
//
LOCUS       DAVKMU010000060         8928 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKMU010000060 DAVKMU010000000
VERSION     DAVKMU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.060"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            32..496
                     /locus_tag="VW343_001829"
     CDS             32..496
                     /locus_tag="VW343_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9756341.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            653..1078
                     /gene="lacA"
                     /locus_tag="VW343_001830"
     CDS             653..1078
                     /gene="lacA"
                     /locus_tag="VW343_001830"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9756342.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1109..1624
                     /gene="lacB"
                     /locus_tag="VW343_001831"
     CDS             1109..1624
                     /gene="lacB"
                     /locus_tag="VW343_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9756343.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1635..2564
                     /locus_tag="VW343_001832"
     CDS             1635..2564
                     /locus_tag="VW343_001832"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9756344.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2566..3546
                     /gene="lacD"
                     /locus_tag="VW343_001833"
     CDS             2566..3546
                     /gene="lacD"
                     /locus_tag="VW343_001833"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9756345.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3780..4616
                     /locus_tag="VW343_001834"
     CDS             3780..4616
                     /locus_tag="VW343_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9756346.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4652..4969
                     /locus_tag="VW343_001835"
     CDS             4652..4969
                     /locus_tag="VW343_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9756347.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            4971..6665
                     /locus_tag="VW343_001836"
     CDS             4971..6665
                     /locus_tag="VW343_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9756348.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6874..8280
                     /gene="lacG"
                     /locus_tag="VW343_001837"
     CDS             6874..8280
                     /gene="lacG"
                     /locus_tag="VW343_001837"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9756349.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTDYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8370..8582
                     /locus_tag="VW343_001838"
     CDS             8370..8582
                     /locus_tag="VW343_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9756350.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
BASE COUNT         2870 a         1501 c         1831 g         2726 t
ORIGIN      
        1 taaagaaact agcaaggtca ggccttactt tattggtgga catccaggat ttaattgtcc
       61 tctacttgat gatgaagtct atgaagatta ttatctagag tttgagaaag aagagacttg
      121 ctctgttcca cgtcctttcc cagaaacagg tatgttggat ttccaagata gaagtccatg
      181 gctagaggga caaaaagaaa tagatcttag ttatgatctt ttcagcaaag atgcagtgac
      241 tttggatgaa ttgcaatctc gaacaattgc ccttcgttct cttaaacatg ataggggatt
      301 gaaagtgcat tttgcagaat ttccaaacct catcatctgg tcaactttga acaagggacc
      361 tttcattacc tttgaaccat ggtctggctt gtcaacattc cttgaagaag gagatcattt
      421 agaagataag aagaatgttt gtctcttaga agccaatcag gttgaagaat tagggtttga
      481 gatagaagtt ttataaaaaa gtaaaaaata aataacaaaa acaaacacaa actgttgact
      541 tcttcaaaca atagtagtat attatgtttg tgaggactaa ttgactgttt tttaaagaca
      601 gccaatacac tgctggaatc cagcatagaa aaaataaaaa ggagtataca atatgtctat
      661 tgttatcggt gcagatgctg caggtttgag attgaaagaa gttgtgaaag acttcttaga
      721 aaaagaaaac ttccaccttg tggatgttac agctgaaggt caagactttg ttgatgtgac
      781 tctcgctgtt gctgcagaag taaacaaaga agaacaaaac cttggtatcg tgattgatgc
      841 ttatggagct ggtccattta tggttgcaac taagatcaaa ggaatggttg ctgcagaagt
      901 ttctgatgaa cgttcagctt atatgactcg tggccacaac aactcacgta tgatcactat
      961 gggtgcacaa cttgttggtg atgaattggc taaaaacatc gctaaaggat ttgttaatgg
     1021 taaatacgac ggtggtcgtc accaaatccg ggttgacatg ttgaacaaaa tgggctaatt
     1081 agattatagt aagaaaggta agttaaaaat gagaattgca attggatgtg accacatcgt
     1141 aacagatgaa aaaatggcgg tttcagaatt tttgaaatca aaaggatatg aagtcattga
     1201 ctttggtaca tatgaccata cacgtactca ctacccaatc tttggtaaaa aagtagggga
     1261 agctgtaact agcggtcaag ctgatcttgg agtatgtatc tgtggtactg gtgttggtat
     1321 caacaacgct gtaaataaag ttccaggtgt tcgttctgcc ttggttcgtg atatgacaac
     1381 agccctttat gctaaagaac aattgaacgc taacgttatt ggttttggtg gtaaaattac
     1441 tggtgaattg cttatgtgtg atatcatcga agctttcatc catgctgaat acaaaccaac
     1501 tgaagaaaac aaaaaattga ttgcgaaaat tgaacatgtt gaaagtcaca atgctcaaca
     1561 aacagacgca aacttcttca ctgaattcct tgaaaaatgg gatcgcggag aataccacga
     1621 ctaagaggta acctatgatt ttaacagtta caatgaaccc atccattgat atttcctatc
     1681 ccttggatga gttgaagatt gatactgtca atcgtgtggt ggatgtaacc aaaacggctg
     1741 gtggtaaggg actcaatgtt acccgagtac tttcagaatt tggcgattct gttcttgcta
     1801 ctggtttagt gggtggcaaa cttggtgagt ttttggttga acatatcgat aatcaagtaa
     1861 agaaagattt cttctcaatt cagggagaaa ctcgtaactg tatcgctatt ctccacggag
     1921 acaaccaaac agaagttctt gaaaaaggtc ctgaagtatt ggaacaggaa ggtcaagact
     1981 ttttggaaca tttcaaaaaa ctcttggagt cagttgaagt agtggccatc tcaggtagtc
     2041 tgccagctgg ccttccagtt gattactatg cgagcttggt agaacttgct aatcaagctg
     2101 gcaagcctgt agtcttggac tgctcaggtg cagcacttca ggctgttctt gaatcacccc
     2161 ataaaccaac agtcatcaaa ccaaataatg aagaattgtc tcagcttctt ggaagagaag
     2221 tttctgagga tttggatgaa ttaaaagaag tacttcaaga acctttgttt gcagggattg
     2281 aatggattat cgtttcactt ggtgccaacg gtacttttgc caaacatggt gacactttct
     2341 acaaggtaga tattcctaga attcaggtgg taaatcctgt tggatctgga gactctactg
     2401 tggcaggaat ttcttcagga cttcttcaca aagaatcgga tgcagaatta ctcatcaagg
     2461 caaatgtcct tggtatgctc aatgctcaag aaaaaatgac tggtcatgtc aacatggcca
     2521 actatcaagt tctatatgat caattaatag taaaagaggt ataaaatggc tttaacagaa
     2581 caaaaacgtg tacgcttaga aaaactttct gatgaaaatg gtatcatctc agctcttgca
     2641 tttgaccaac gtggtgcttt gaaacgcctc atggctcaac accaaacaga agaaccaact
     2701 gtggcccaaa tggaagaact taaagtcttg gtagcagatg aattgactaa atacgcttca
     2761 tctatgcttc ttgaccctga gtatggactt ccagcaacta aagctcttga tgaaaaagct
     2821 ggtcttctcc ttgcttatga aaaaacaggt tatgacacaa caagcacaaa acgcttgcca
     2881 gactgcttgg atgtctggtc tgcaaaacgt attaaagaag aaggtgcaga tgcagttaaa
     2941 ttcttgcttt actatgatgt agatagctca gacgaactca atcaagaaaa acaagcctac
     3001 atcgaacgca tcggttctga gtgtgtggct gaagatatcc cattcttcct tgaaattctt
     3061 gcttacgatg aaaaaattgc ggatgcaggt tctgtagaat acgcgaaagt aaaaccacac
     3121 aaagttatcg gcgctatgaa agtcttttca gacccacgct ttaacattga tgtcttgaaa
     3181 gttgaagttc ctgttaacat taaatatgtt gaaggcttcg ctgaaggtga agtagtttat
     3241 acacgtgaag aagcagcagc cttcttcaaa gcgcaagatg aagcaacgaa cttgccatac
     3301 atctacttga gtgctggtgt atcagctaaa ctcttccaag atactcttgt atttgctcat
     3361 gaatcaggtg caaactttaa cggagttctt tgtggccgtg caacatgggc tggatcagtt
     3421 gaagcataca tcaaagatgg tgaaacagca gctcgcgaat ggcttcgcac aactggattt
     3481 gaaaacattg atgagctcaa caaagttctt caaacaacag caacttcatg gaaagaacgt
     3541 gtgtaagaaa gtcctcctag tttaggaaca tgaatctaaa aaaatttaaa aaaagttgta
     3601 tgtaaaggtt tacaaaataa cttacttgtg ctatacttaa atcacaagtt aatacaaggt
     3661 gagtgttact aagtaatatt aggcatgatc acaggtgaat tagaaatcag ctgattttct
     3721 agttcatttg tggtcatttt ttgtactcat atacctttaa gatataaaag gaggttgaca
     3781 tgtatcgaat tctaaatcca atgaatcaca atgtctcgct tgtcagaaat gataagggag
     3841 aagaggtgat tgtaattggt aaggggattg cattcggaaa gaagaagggg gatttgattg
     3901 ctgaaaatca tgttgagaaa atctttcgga tgaagaccga agagtccaga gaaaacttta
     3961 tggctcttct caaagatgtt ccgcttgatt ttatcacagt gacctatgaa atcattgata
     4021 agctatcaaa gaaatatcat tatccgattc aagagtatct ctatgtaacc ttgacagatc
     4081 atatttactg ttcttatcaa gctctaactc aaggaaggta caaggatagt aatctgccag
     4141 atatttctgc taagtatcct gtcgcttttc aaatcgcaaa tgaagctttt gaaatttact
     4201 gtcagaagct agcagatcat tttcctgagg acgaaattat tcggattgct tatcatttca
     4261 ttaatgctga aggtgaaaat gaagtggaac ttgtggagtc gattgataag aggaaagaaa
     4321 ttctcaggaa tgttgaagaa gttttaacgg actatgcaat tcaacgaact aaaaagaata
     4381 accatttcta tgatcgcttt atgatccatt tgaattattt cttggattat ttagacagat
     4441 ctagagatga taaccaatca cttctggata tggaagatca tattaaacaa tcctatccaa
     4501 aagccttcga gattggttcc aagatctatg atgtgattac gcaacatacg ggtcttgatt
     4561 tgtataaaag tgaacgagtt tatctagttc tacatatcca acgtttattg tcataaaaat
     4621 ttatttaaaa ctatataagg agaattctat catgaataga gaagaagtaa cattgttagg
     4681 ttttgaaatc gtagcctatg ctggcgatgc tcgttcaaaa ctattggaag ccttgaaggc
     4741 tgctgaagct ggtgattttg aaaaagcgga cgctctggta gaggaagctg gtagctgtat
     4801 tgcagaggct caccacgcgc aaacaagtct attgactaag gaagcttctg gtgaggactt
     4861 agcgtatagt gtgaccatga tgcatggtca agatcacttg atgacaacta tcttgttaaa
     4921 agacctaatg caccatttaa ttgaacttta caagagagga gttaaataaa atggataagt
     4981 taataatttt tattgagaaa ggaaagcctt tctttgagaa attatcgaga aatatatact
     5041 taagagcgat aaaagatggt tttatttcca gtatgccagc agtattattc tcaagtattt
     5101 ttatactaat agctgctgtg cccaatattt ttgggtttaa atggtctgat gaacagttag
     5161 cttttatttt gaaaccttac aactactcaa tggggatttt agctctctta gtagctggga
     5221 caactgcaaa atcgttaaca gattcagtta atacacgtag tatggagaaa accaaccaaa
     5281 tcaactacat gtcaactttt ttagcagctg ttgttggatt gttaatactg gcagctgatc
     5341 caattgaagg tggatttgct aatggtcttt taggtactag aggattactt actgcatttt
     5401 tggcagcttt tattacagta aatatctata aagtatgtat taaaaataat gtaaccatta
     5461 gacttcctga agaggttcca ccaaatattg cacaagtttt taaagatgtt attccgtttg
     5521 ccttatctgt tctttcaata tatggattgg atttaattgt tagaaatatt tttggtacaa
     5581 atgtagcaga atctgttggt aaaatattag cccctctatt ttcagctact gatggttata
     5641 tcggtttagc tattgtattt ggtgcttatg ctttcttttg gtttgttgga attcatgggc
     5701 catctgttgt agaaccttta attgtagcta tcagttatgc aaatatagaa gctaatgttc
     5761 agcttgtgca agctggtatg cacgcagata aaattttaaa tccagttact caaacttttg
     5821 ttgtaacaat gggtggtact ggtgcaacac tagtagttcc atttatgttt atgtggttat
     5881 gtaaatcaaa aagaaataga attgtcggac gtgcgtctgt agttcctact ttctttggcg
     5941 taaatgaacc aatcctattt ggtgctccaa ttgttttaaa tcctatcttc tttattccgt
     6001 ttgttactgc tcctattatc aatgtttgga ttatgaaatt ttttgtagat gttctacaaa
     6061 tgaatagttt cagtattatt ttaccttgga caacacctgc tccaattggt attgttatgg
     6121 gaacggcgct ggctccattg tcattcgtct tggctataac tttaatcatt atcgacactt
     6181 taatctatta tccatttgtt aaagtttatg accatcaaat tttagaagaa gaacggaaag
     6241 gaaactcttc atctgaattg aaagaaaaag ttgctgcaaa cttcaacact gcaaaagcgg
     6301 atgctattct tgaaaaagcg ggtgtagaag cagcacaaaa tacaatcact aaagaaacaa
     6361 atgtcctcgt tctctgtgca ggtggaggaa caagtggtct ccttgcaaat gctttgaata
     6421 aggcagcagc agaatacaat gtccctgtga aagcagcagc aggcggctat ggtgctcacc
     6481 gtgaaatgtt accagagttt gatcttgtta tccttgcccc tcaagttgct tcaaactttg
     6541 aagatatgaa agcagaaaca gataagctcg gtattaaact agcgaaaaca gaaggcgctc
     6601 aatacatcaa attaactcgt gatggaaaag gtgctcttgc attcgtacaa gcgcaattcg
     6661 attaaggcta gagactctga aatagtctcc tatcgttacg gaaatcgcta tggcgaattt
     6721 cctattatta attcgtcggt aaaaagatat cgtttttacc tcctcatgtc acaattcggt
     6781 gacttggtac aagaagtgag atggagaagg atggctcatt gactcctctc ctctcacttt
     6841 tactttattt aaatcaagaa ataggtgaaa aaaatgacaa aaacacttcc aaaagacttt
     6901 atttttggtg gcgcaacagc tgcctatcaa gcagaaggtg ctacacatac tgatggtaaa
     6961 ggaccagtag cgtgggataa atatcttgaa gataactact ggtacactgc agaaccagcc
     7021 agtgattttt acaatcgata tccagttgac ctcaagctag cagaagagta tggtgtcaat
     7081 ggtattcgaa tttctattgc ttggtcacgt attttcccga ctgattacgg ccaagtaaat
     7141 gctaaaggtg ttgagtttta tcataattta tttgcagagt gtcacaaacg tcatgttgag
     7201 ccttttgtaa ctcttcatca ctttgacacg ccagaagctc tccactcaaa tggagacttc
     7261 ttaaaccgtg aaaatatcga acattttgta gactacgctg ccttctgttt tgaagaattt
     7321 ccagaagtaa actattggac aacctttaat gaaattggac caatcggtga tggtcaatat
     7381 ttggttggga aattccctcc aggtatccag tacgaccttg ccaaagtctt tcaatcacac
     7441 cacaatatga tggtgtctca tgcacgcgcg gtaaaattgt acaaagagaa aggctataaa
     7501 ggggaaattg gtgttgttca cgccctgcca actaaatatc ctctagatcc tgaaaatcca
     7561 gcagatgttc gtgcagctga gttggaagat atcatccaca ataaattcat cttagacgca
     7621 acttatctag gtcgctattc agctgaaacc atggaaggtg tcaaccatat cttatcagtc
     7681 aatggtggta gcttggatct tcgtgaagaa gattttacag cattagaagc tgcaaaagac
     7741 ttgaatgatt tcctaggaat caactactat atgagtgact ggatggaagc ctttgatgga
     7801 gaaactgaaa ttatccataa tggtaaaggt gaaaaaggaa gctctaagta tcaaatcaaa
     7861 ggtgttggtc gtcgtgtagc tcctgactat gtaccacgca cggattggga ttggattatc
     7921 taccctcaag gtttgtatga ccaaatcatg cgtgtgaaga aagattatcc taactacaag
     7981 aagatttaca tcactgaaaa tggtctcggc tataaagatg agttcgttga taacactgtt
     8041 tacgatgatg gtcgtattga ttacgtgaaa caacacttgg agatcttgtc tgatgcgatt
     8101 gcagatggag ctaatgtaaa aggttacttc atttggtcat taatggatgt cttctcatgg
     8161 tcaaacggtt atgagaaacg ttatggtctc ttctacgtag attttgaaac tcaagaacgt
     8221 tatcctaaga aatcagctca ctggtacaag aaagtagcgg aaactcagat tatagactag
     8281 tagaattagt cattagatat agaattttag tgagtcaaaa agatgttcaa agattttatc
     8341 caatctattt atgaaaaaaa gtttatatta taaatttcga aaaatgctct caaataccgt
     8401 gtttgacgag tgaagaattg aaaagtcttg gaaaatggta tgtctcgact ggtaaagaat
     8461 ggatttgtca ttcagatgat gagctggaag aatttaaaaa tctattttta aattttatca
     8521 atcctgaaga atgggatact atctcctttg attcagattt tatgccgttt caacaatcgt
     8581 aaccaatttc tcaaaaaagt taaatcttat atttagtact ctgtaaaact cttatctaat
     8641 cacgttgctt atactcaatg aaaatcaaag agcaacttta aactaggaag cgagtcgcag
     8701 atttctcaat gcatagcttt gaggaattgg gcaaaaagtc tttgatatag aaaaaagcat
     8761 agtatcaggt gttgaaacac ctgatactat gctttttatt gtgggaagat ttactttttt
     8821 tcttctgaaa ttgagttgtt acccaggctc tttcagttta ttaaggcttg atgactttaa
     8881 tgtgtttaga tagcttaaaa aggattgaat cacttagttt agaatctg
//
LOCUS       DAVKMU010000061         8924 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKMU010000061 DAVKMU010000000
VERSION     DAVKMU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.061"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            122..277
                     /locus_tag="VW343_001839"
                     /pseudo
     CDS             122..277
                     /locus_tag="VW343_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VW343_001840"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VW343_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9756351.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYIDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERKNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VW343_001841"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VW343_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9756352.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGCQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFCFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     HVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7614..8792
                     /locus_tag="VW343_001842"
     CDS             7614..8792
                     /locus_tag="VW343_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756353.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSIC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDYRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2762 a         1863 c         1890 g         2409 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct acccgtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgaatt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaagggc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc gccttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgctga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcccgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga cttgtatatt gacctcagtc
     1681 agtctgatat tgatgctttt gaacaatata tccgctatct tggtattaat ggcttgccag
     1741 cctttcagca aaccttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttttg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggaaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatcagca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcat cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaagttgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg tagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggttgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc gccagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtacttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgtgctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag atagcgattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagaaaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatcctcaa gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac atgcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggctgaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg gattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgcct tggttgccct tatgaagtct ccaatgtttt gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggcttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 accccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaatcca aggaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacacgttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agtactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     7501 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag ctgactgcgg
     7561 ctagctttct agtttgctct ttgattttta ttgagtatta gaaaggagac ctcatgacac
     7621 ttccagttag aaaatccctg cacgatgcag ttttacaggc ttcgaaagcg gatacttggg
     7681 aacaagctac caaggaatgg aatgaagttt ccttgatttt taacggtatc ggccgtagca
     7741 tttgtgtctg tggaaatgtc atcaaatacg cttatgaact ctttaacggt gtcacaggcc
     7801 aacgcctctt tccaattggt agcgactgtg ttcgtcattt tcatcgattg tcccttgacc
     7861 agcaattgga agaggaagaa aaactgctca gaaaggttga aaatctaacc agaaaagctc
     7921 agaaaaagga aaaaatcaag gtcaataaaa gtgactttga cgagcgactt ctaaaatggc
     7981 tctgggaaaa aggtgttttc aaacccaatc gtggcaatca atttgcgcct gagagagact
     8041 accagctttt cctagaagtc tttcagggag gaagttggac caaggcggaa ccaaagaaaa
     8101 aggctcggat ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt gggaaatcgg
     8161 atgaccaact ctaccttgtc aagctaggca aggagaaaat tgactacgaa caagaactcc
     8221 gtatccaggc agagaaagaa cgcaagaaga gggataaaat cgccaagcaa tatgcagaca
     8281 atctggttct agccatggga cccgcagagc gtgcttatca agattacttt ggctttacag
     8341 agaccctgac tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa aatagagcag
     8401 aacgggctat taaggccaaa caataccaaa aagagctgga aaaagaccaa cgaattgcca
     8461 gtcaggatcc aattgaaaga aaacagaaac agacttggct cctcaattcc tattttcgtg
     8521 agcttcctga agaaaaagct cgattttcta ggctcttatt agaatatcga aaaaatggtg
     8581 aagtaccctt ttcaactgaa tacctgtcag atcatctcat cgactttttc tataaaatga
     8641 aagcctttga atttgaaatc gcaccagaac aagtccgaga ttttctaaaa aaaagcctgc
     8701 aggaagacta tcgctcatca gcacaaggaa gctggataga aggaattctc ctcaactgcc
     8761 tcaaaccatt tttagaacga ttagtgatat gagtaacacc taccgtgtaa atttcatggt
     8821 aggtgttatt gtacccccaa ataatataaa acaaagtatg aaagcgcatc tcaattttaa
     8881 gacttttttg taaattaaga gtataattaa ggtatactgc cttt
//
LOCUS       DAVKMU010000062         8666 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKMU010000062 DAVKMU010000000
VERSION     DAVKMU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.062"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..586)
                     /locus_tag="VW343_001843"
     CDS             complement(<1..586)
                     /locus_tag="VW343_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9756354.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQR"
     gene            complement(874..1836)
                     /gene="nrdF"
                     /locus_tag="VW343_001844"
     CDS             complement(874..1836)
                     /gene="nrdF"
                     /locus_tag="VW343_001844"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9756355.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(2024..4183)
                     /gene="nrdE"
                     /locus_tag="VW343_001845"
     CDS             complement(2024..4183)
                     /gene="nrdE"
                     /locus_tag="VW343_001845"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9756356.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4264..4482)
                     /gene="nrdH"
                     /locus_tag="VW343_001846"
     CDS             complement(4264..4482)
                     /gene="nrdH"
                     /locus_tag="VW343_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9756357.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
     gene            4833..5096
                     /locus_tag="VW343_001847"
     CDS             4833..5096
                     /locus_tag="VW343_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9756358.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            5102..6835
                     /gene="ptsP"
                     /locus_tag="VW343_001848"
     CDS             5102..6835
                     /gene="ptsP"
                     /locus_tag="VW343_001848"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9756359.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            7090..>8666
                     /locus_tag="VW343_001849"
     CDS             7090..>8666
                     /locus_tag="VW343_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9756360.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKL
                     VDDILAFLAPIRHPERLG"
BASE COUNT         2674 a         1641 c         1813 g         2538 t
ORIGIN      
        1 tgcgttgagc ctctccctct tcctcgctaa aagtagctag agctccgttt tgaataccat
       61 tacagctaac gaaagcttta gaaaattgga gattagagag attttgtagg gtcaatgtgc
      121 caacaaaagc acctgtaata tcgcgataat ttccacctat caaaatcaaa tctgttaatt
      181 ttcgttcgct taaaatcaga aaaacaggta gactgttggt tacgacgcgg atattgtcaa
      241 taggcaactc acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa atagtttctc
      301 tttcttctac tagactgcct gcaaaatggg ctatttcttg tttttctgcc gtttggaggg
      361 cttgtttttc aatatttgat cgctcattag tcaaaaggga gttggttcga agtttttcag
      421 ctccaccatg cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa tagcgccttg
      481 cagtcatatc tgaaacggct atttcgtcca taatctgttt aactgttatg gttcctttac
      541 tatttactat ctctaaaatt ttggttaatt tttcttgttt gagcatatta tcacctcgtt
      601 tcctactact atcttaccat aaacaaactc atcattcaaa tacaaaaaca acaaaatgaa
      661 acaaaaacaa aaatatcgaa gtttgttttc aaaactttcg atatttttgt tgggttatta
      721 ttttgttgtt tctagtttac tttttgatgg ttaagagtgg tggagaatta tactcaatga
      781 aaatcaaaga gcaaactagg aagctagccg caggctgtac ttgagtacgg caaggcgaag
      841 ctgacgtggt ttgaatttga ttttcgaaga gtattagtcc aaaccgtagt tgtagtcatc
      901 atcttgcatg gcttcaactt cgccaagaag gtaaccattt ccgacttgag agaagaagtc
      961 atggttggaa gttcctgttg aaataccgtt cataacgatt gggttgacat cttcagctga
     1021 atctgggaaa agtggatctt gtcccatgtt catgagagct ttattggcat tgtagcgaag
     1081 gaaggtttta acctcttcag tccaaccaac accgtcataa agactctctg tgtagccttc
     1141 ttcattttca taaagagtat agagtaggtc gtacatccat tctttgagtt tttcttgctc
     1201 ttcttcaggt aattcattga aaccaagttg gaatttgtaa ccaatgtagg ttccgtgaac
     1261 agactcgtca cgaataatca atttaatgat ttctgcaacg ttggcaagtt tgttgttacc
     1321 gagatagtag aggggagtga agaaaccaga gtagaagagg aaggtttcga ggaagacgct
     1381 ggcaactttc ttttcaagtg ggctgccgtt taggtagatt tcgttgacaa tctcagcctt
     1441 cttttgtagg taaggattgg tattggtcca ttcgaaaatt tcttcaatct cagccttagt
     1501 attcaaggta gaaaagattg atgagtaaga tttagcgtgg acagattcca taaattggat
     1561 gttattgaaa acagcttcct catgtggtgt acggatgtct gcgcgaaggg cttgaacccc
     1621 agtttcagat tgcatagtgt caagaagggt taaaccacca aaaacttttc cgaccaagtc
     1681 tttctctttg ttagatagct ttctccagtc atccaagtcg tttgataagg gaatacgtgt
     1741 atcgagccaa aattgctccg tcagtttttc ccaagttgat ttgtcgatga catcttcgat
     1801 ggcattccag ttaatggctt tgtagtaagt ttccatttaa aatctctttc tgtgtttagt
     1861 attgcgaact cacaattatt tctactttac cataattcta taggagtatc gcacaaaaag
     1921 tcggaagccc gacttttaaa atgttacata aattatgtta tgacatagta gatttgattt
     1981 tatcagtgct gcttagggaa aaatagtgtt tctatgctag aaactaaatc acacagcttt
     2041 cacattggtt ggcgccgact tctccaccgt catctgtaaa ggtacggacg tagtagatag
     2101 acttgattcc cttgttaaag gcatagttac gaaggatgga caagtcacgt gtcgtttgtt
     2161 tattttccct cttccattcg taaaggcctt ttggaatgtc actacgcatg aagagggtga
     2221 gtgaaagtcc ttgatccacg tgttcagtcg cagcagcgta aacatcgatg actttacgca
     2281 tatccatatc gtaggcagaa gtgtagtaag gaatggtttc tgtagacaag ccagcagcag
     2341 ggtagtagat tttaccaatt ttcttttcct gacgttcttc aatacgttgc gtaatcgggt
     2401 ggatagaagc agaaacatcg ttgatgtagc tgatagaacc atttggcgct acagcaaggc
     2461 gattttggtg gtaaagacca tcctcttgaa ccttgtcgcg aagttcagtc caatcagcaa
     2521 caccagggat aaaaacattt ttgaagagtt ctttaacacg gtctgatgtt ggaacaaatt
     2581 caccagttac atacttgtca aagtaacttc cgttagcata gtctgatttt tcaaagttgt
     2641 ggaaggtaat accacgttca cgcgcaatat tgtttgactc taccaaggtc cagtagttca
     2701 taagcataaa gtagatgctt gtaaattcaa cagactcagg tgaaccatat tcaatgagtt
     2761 gttgggcaag gtagctgtgc agtcccatgg caccgagacc aaaggtgtgg gcttggctat
     2821 ttccatggtc aatcgttggt acagctacga tatgtgaact atctgtaacg aaagtaaggg
     2881 cacgaaccat agcacggata gaacgaccaa aatcaggtga agtcatcatg ttaaccacgt
     2941 tggttgaacc caggttacat gaaacatctg ttcccatttg aaggaattct tgagcatcgt
     3001 tgatcaagct tggttcttga acttgaagaa tctcagaaca caagttactc atgataatct
     3061 ttccatcgac aggatttgca cggttagccg tatctatgtt gactacataa ggatagccag
     3121 actcttgttg caatttagag atttcagttt ccaaatcccg cgccttgatt tttgtcttgc
     3181 gaatatttgg atttgcgacc aattcatcgt atttttcagt aatgtcgatg taattgaatg
     3241 gcacaccgta ttctttttct acagagtaag ggctgaagag gtacatttct tcatttttac
     3301 gagccaattc gtagaattta tcaggtacta caacaccaag tgatagagtc ttgacacgta
     3361 ctttttcatc agcgttttct ttcttagttg aaaggaaagc gatgatatct gggtgaaaga
     3421 cgttgaggta gacaacaccg gcaccttgac gttgccccaa ttggttggag taagagaagc
     3481 tgtcttcaaa aagcttcata acaggaacga cacctgaagc agctccttca tagcctttga
     3541 taggtgcacc agcttcacga aggttgctga gggtaattcc cacaccacca ccaatacgtg
     3601 aaagttgaag agctgagttg atagaacgtc cgatagagtt catatcatcc gtcacttgga
     3661 ttaggaaaca agataccaac tccccacgac gagcacgtcc agcattcaag aaggaaggag
     3721 tagcaggttg gtagcgttgg tggatgattt cattggcaat atcgattgca acagcttcat
     3781 tcccatcagc gaaataaagg gcgttgaaga agacacggtc ttccatattt tcaagatagt
     3841 attcaccgtc attagtcttt aaggcatatt gattgtaaaa tttataagct gccatgaatg
     3901 acttgaattg gaagttttgg tctttgataa attgagccaa ttcttccaag aactctggac
     3961 ggtatttctt gataaaggct gtttcgatgt agttgtgttc aatgaggtaa ttgattttgt
     4021 ctttgattga atcaaaaacc atagtgtttg gaactacatt ttctttaaag aaagcatcca
     4081 aggcttcctt atctttatga agcatgattt gtccattaac aggacggtta atttcgttat
     4141 taagacggaa gtaagtcacg tcttcaagat gttttaatcc cataaaattt cccttatcta
     4201 attacaaaag aaaggcttct aagttagccc taaaagcagt ttcttctgga tgatgtacta
     4261 agattatgct aattgtttca gtttttctgg ttggaaacct gaaaagactt cagttggtgt
     4321 ttggataaca ggagctgcgc taaaaccgag ctctttaact tgatcgacgt actcaggttg
     4381 ctcatcaaga ttgatttcac gataagagac attattactg tccaagaaac gcttggtcat
     4441 tttacattgg acacaattgt ttttagaata aacggttacc attgtgtaac tcctcttcaa
     4501 aatttaatac tatcttagta tatcagaaaa taaaattttg tcaatgattt aaactaaata
     4561 tagtggtgtt cttttgttgt ttttatatat aaacacaata tatagtgttt gtattgtgaa
     4621 atagcaatga ttatcctata aattgttttt gaagaaacta tatcctgtgt tttgttatct
     4681 aggatagaag attttcagca agttatctga aaggaaattg aaggaaaatc cagaaaatgc
     4741 ttgaaaaaaa tcctagaaga tggtataata ctaaattgta agggttatca catataactc
     4801 aaaaaaagaa agaacaaaag gagagtcaaa ctatggcttc taaagatttc cacgtagtgg
     4861 cagaaacagg tattcacgca cgtccagcaa cattgttggt acaaactgct agcaaatttg
     4921 cttcagatat cactcttgag tacaaaggta aatcagttaa ccttaaatca attatgggtg
     4981 ttatgagtct tggtgttggt caaggtgctg acgtaactat ctcagctgaa ggtgcagatg
     5041 cagatgacgc tatcgctgca atctcagaaa caatggaaaa agaaggattg gcataaggga
     5101 aatgacagaa atgcttaaag gaatcgcagc atctgacggt gttgcagttg caaaagcata
     5161 tctactcgtt cagccggatt tgtcatttga gactattaca gtcgaagata caaacgcaga
     5221 agaagctcgc cttgatgccg ctctacaggc atcacaagac gagctttctg ttattcgcga
     5281 gaaagcagta ggtacgctcg gtgaagaagc agctcaagtt tttgatgctc acttaatggt
     5341 tcttgctgac ccagaaatga tcagccaaat caaggaaact atccgtgcga agaaagtgaa
     5401 tgcagaagca ggtctgaaag aagttacaga tatgtttatc actatctttg aaggcatgga
     5461 agacaaccca tacatgcaag aacgcgcagc ggatatccgc gacgtgacaa aacgtgtatt
     5521 ggcaaacctt cttggtaaaa aattgccaaa cccagcttct atcaatgaag aagtgattgt
     5581 gattgcgcat gacttgactc cttcagatac agctcaattg gacaaaaact ttgtaaaagc
     5641 ttttgtaacc aacattggtg gacgtacaag ccactcagct atcatggcac gtacacttga
     5701 aattgctgct gtattaggta caaacaacat cactgaaatc gttaaagacg gtgacatcct
     5761 tgctgttaac gggatcactg gagaagtgat tatcaacccg acagatgaac aagcggcaga
     5821 atttaaagca gctggtgaag cctatgcgaa acaaaaagct gaatgggcac ttttgaaaga
     5881 tgctcaaaca gtgactgctg acggtaaaca cttcgagttg gctgccaata ttggtactcc
     5941 aaaagacgtt gaaggtgtta acaacaacgg tgcagaagct gttggacttt accgtacaga
     6001 gttcttgtac atggattctc aagacttccc aactgaagat gagcagtatg aagcatacaa
     6061 ggctgttctt gaaggaatga acggtaaacc tgttgtcgtt cgtacaatgg atatcggtgg
     6121 agataaggaa cttccttact tcgatatgcc tcacgaaatg aacccattcc ttggattccg
     6181 tgctcttcgt atctctatct ctgagactgg agatgctatg ttccgcacac aaatccgtgc
     6241 tcttcttcgt gcgtctgttc acggtcaatt gcgtatcatg ttcccaatgg ttgcgctctt
     6301 gaaagaattc cgtgcagcga aagcagtctt tgatgaagaa aaagcaaacc ttcttgctga
     6361 aggtgttgca gttgcggata acatccaagt tggtatcatg atcgagattc ctgcagcggc
     6421 tatgcttgca gaccaatttg ctaaagaagt tgacttcttc tcaattggta caaacgactt
     6481 gatccaatat acaatggcag cagaccgtat gaacgaacaa gtttcatacc tttaccaacc
     6541 atacaaccca tcaatcctac gcttgattaa caatgtgatc aaagcagctc acgctgaagg
     6601 taaatgggct ggtatgtgtg gtgagatggc tggtgaccaa caagctgttc cacttcttgt
     6661 cggaatgggc ttggatgagt tctctatgtc agcaacatct gtacttcgta cacgcagctt
     6721 gatgaagaaa ctcgacacag ctaagatgga agagtacgca aaccgtgccc ttacagaatg
     6781 ctcaacaatg gaagaagttc ttgaacttca aaaagaatac gttaattttg attaatcgaa
     6841 aagtccctgc aactcagtta cagggacttt ttttgatatt ttaaaaagaa ttttcaagaa
     6901 aatctttctt ataaaaagtc caaccttgaa aaagtagtgg tcagaacaaa aaatacttaa
     6961 atggttcata aaattcttga caagttggag atttaggagt aaactattaa ccagttaagt
     7021 aatagagagg agtttctgca atttagaaat gaattgcaac tagaaatatc aaatagaaag
     7081 agagtttcga tgaaaattaa taagaaatac cttgttggtt ctgcggcagc tttgatttta
     7141 agtgtttgtt cttacgagtt gggactgtat caagctagaa cggttaagga aaataatcgt
     7201 gtttcctata tagatggaaa acaagcgacg caaaaaacgg agaatttgac tcctgatgag
     7261 gttagcaagc gtgaaggaat caatgctgag caaatcgtca tcaagataac agaccaaggc
     7321 tatgtcactt cacatggcga ccactatcat tattacaatg gtaaggttcc ttatgatgcg
     7381 attttcagtg aagaattact catgaaagat ccaaactata agctaaaaga tgaggatatt
     7441 gttaatgagg tcaagggtgg atatgttatc aaggtagatg gaaaatacta tgtttacctt
     7501 aaggatgctg cccacgcgga taacgtccgt acaaaagagg aaatcaatcg acaaaaacaa
     7561 gaacatagtc aacatcgtga aggtgggact ccaagaaacg atggtgctgt tgccttggca
     7621 cgttcgcaag gacgctatac tacagatgat ggttatatct ttaatgcttc tgatatcata
     7681 gaggatactg gtgatgctta tatcgttcct catggagatc attaccatta cattcctaag
     7741 aatgagttat cagctagcga gttagctgct gcagaagcct tcctatctgg tcggggaaat
     7801 ctgtcaaatt caagaaccta tcgccgacaa aatagcgata acacttcaag aacaaactgg
     7861 ctaccttctg taagcaatcc aggaactaca aatactaaca caagcaacaa cagcaacact
     7921 aacagtcaag caagtcaaag taatgacatt gatagtctct tgaaacagct ctacaaactg
     7981 cctttgagtc aacgccatgt agaatctgat ggccttgttt tcgacccagc gcaaatcaca
     8041 agtcgaaccg ccagaggtgt agctgtccct catggtaacc attaccactt tatcccttat
     8101 gaacaaatgt ctgaattgga aaaacgaatt gctcgtatta ttccccttcg ttatcgttca
     8161 aaccattggg taccagattc aagaccagaa caaccaagtc cacaatcgac tccggaacct
     8221 agtccaagtc tgcaacctgc accaaatcct caaccagctc caagcaatcc aattgatgag
     8281 aaattggtca aagaagctgt tcgaaaagta ggcgatggtt atgtctttga ggagaatgga
     8341 gtttctcgtt atatcccagc caaggatctt tcagcagaaa cagcagcagg cattgatagc
     8401 aaactggcca agcaggaaag tttatcgcat aagctaggag ctaagaaaac tgacctccca
     8461 tctagtgata gagaatttta caataagact tatgacttac tagcaagaat tcaccaagat
     8521 ttacttgata ataaaggtcg acaagttgat tttgaggctt tggataacct gttggaacga
     8581 ctcaaggatg tctcaagtga taaagtcaag ttagtggatg atattcttgc cttcttagct
     8641 ccgattcgtc atccagaacg tttagg
//
LOCUS       DAVKMU010000063         8597 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKMU010000063 DAVKMU010000000
VERSION     DAVKMU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.063"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..383
                     /locus_tag="VW343_001850"
     CDS             <1..383
                     /locus_tag="VW343_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEU9756361.1"
                     /translation="IERIGDRIVEILKNLQILQNNEILKKIISEIKILHEVIGLHMNR
                     AISCYREEQSGCLDMVVIQKQNEIEELSINMEKKIMNYIFEDDGNVSEVISALDIIHH
                     LDKIAHTTQAIYKWIMYRKYGNIN"
     gene            396..1871
                     /gene="galT"
                     /locus_tag="VW343_001851"
     CDS             396..1871
                     /gene="galT"
                     /locus_tag="VW343_001851"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9756362.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1884..2894
                     /gene="galE"
                     /locus_tag="VW343_001852"
     CDS             1884..2894
                     /gene="galE"
                     /locus_tag="VW343_001852"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9756363.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNIFE
                     SVNKIPIPYKIVPRRSGDVATYYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            3058..3717
                     /locus_tag="VW343_001853"
     CDS             3058..3717
                     /locus_tag="VW343_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9756364.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3742..4809
                     /locus_tag="VW343_001854"
     CDS             3742..4809
                     /locus_tag="VW343_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9756365.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4913..5923
                     /locus_tag="VW343_001855"
     CDS             4913..5923
                     /locus_tag="VW343_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756366.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5935..7626
                     /locus_tag="VW343_001856"
     CDS             5935..7626
                     /locus_tag="VW343_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9756367.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7629..8339
                     /locus_tag="VW343_001857"
     CDS             7629..8339
                     /locus_tag="VW343_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9756368.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2925 a         1171 c         1685 g         2816 t
ORIGIN      
        1 acattgaacg tattggggat agaattgttg aaattttaaa aaatctacag attcttcaaa
       61 ataatgaaat tttgaagaaa attatttcag aaataaaaat acttcatgaa gttatcggac
      121 tgcatatgaa tagggctata tcttgttata gagaggaaca atctggatgt ttagatatgg
      181 ttgttataca aaaacagaat gagatagaag agcttagtat aaatatggaa aagaaaataa
      241 tgaattacat atttgaagat gatgggaacg tttctgaagt aattagtgct ttagatatta
      301 ttcatcacct tgataaaatt gctcatacta cgcaagcaat ttataaatgg ataatgtata
      361 gaaaatatgg gaacataaat tagaaagaaa aatatatgaa aacaattatt gataattttg
      421 ttgatagagt aattgaatac ggaatgtata atgaaattga caaaatatat gtgaaaaata
      481 gagtactagc gttaataggt gaggagggaa ttgatcgcat ttccgatgaa aatgacctga
      541 aacaaataaa agattactta gtggaaatcg ccttaaaaaa cggtaaaatt aaagatttaa
      601 tcgaggaaaa ggaatgttta ggagcagagt taatgaattt catagttcct ttacctagtc
      661 gattaaatga tattttttgg agttcatatg atatatcacc tcaagaagca gttgaagagt
      721 tttataagtt aagtaaagat agtgattata ttaaaacttc tgctattgct aagaatattg
      781 agtttcgagc atcaactaaa tacggagagc tagagattac gataaatttg tcaaaacccg
      841 aaaaagatcc aaaaacaatt gctgctgaaa aattagtaaa agcaactaat tatcccaaat
      901 gtttactatg tatggaaaat gaaggatatc aggggcgaat aaattatcca gcacgttcga
      961 atcatagaat tattcggttg aaacttggtg acgaagtatg gggattccag tattctccct
     1021 actcatactt taatgagcat gcgatatttt tgaatagtca acatgtacca atggctatta
     1081 cctctaaaac atttgaacaa ttattggaga ttgttgatat tttgccagga tattttgctg
     1141 gttcaaattc tgatcttcct atctcaggag gctccattct tagtcataac cactaccagg
     1201 gagggaaaca tattttccca atggaaaaag caaaatttga gagtgaattt tgttttaaag
     1261 actttgagga tgttaatgct ggtattgtaa aatggccaat gtcagtaatt aggttacaaa
     1321 gtgaaaataa aaatcgcttg ttagatttag ctactaaaat tctaaataag tggagagaat
     1381 attcagattt agaagtagac atcattgcta tgactgaaga tgtcccacac catacggtga
     1441 ctcctattgc tcgtaaagtg gacgggagat acgaattaga tattgttcta agagataatc
     1501 atactactga acaataccca gatggtgttt tccatcctca tcaagatgta caacatatta
     1561 aaaaggaaaa tatcgggtta atcgaagtga tgggacttgc gatattacca cctcgtttga
     1621 aaccagagtt agaagaagtt ggaaaatatc ttttaggaga ggataatgca attgcggatt
     1681 atcatttaga atgggccgac caattgaaag aaaaatatcc tcgcattaat aaagaagagg
     1741 ttaatagcgt agttcaacac gaagcaggac aagtgtttgc gagagttctg gaagatgcag
     1801 gagtttataa gaatacaccg tctgggcatg aagcgtttat gagatttgtt aagtcggttg
     1861 gaattaatta ggaggaaacc cagatggcaa tattggtaac aggcggagct ggttatattg
     1921 gtagccatac cgtagtagaa ttactaaatt taggaaagga agttatcatt gtcgacaacc
     1981 tttcgaactc tagcaaattg gtgctagacc gtattgaagc aattacagga atacgtcccg
     2041 tgttttacga attagatgtt tgtgataaac aagcattgag aaaggtattt gaacaagaat
     2101 cgattgatgc tgcaattcat tttgcaggtt ataaagctgt cggagaatcc gtgcaaaagc
     2161 ctgtgatgta ctacaaaaat aatattatga gtacattggc acttgttgaa gtgatgtcag
     2221 aatttaatgt taaaaagatt gtcttttctt caagtgcgac tgtatatgga attaacaatc
     2281 agtcacctct aattgagacg atgcaaacaa gtgcgacaaa tccttatggg tatacgaaag
     2341 tgatgcttga gcaaatttta aaagatgttc atgtggcaga ttcagaatgg agtattgcgt
     2401 tgcttcgtta tttcaatcca attggtgctc atgagtctgg tttgattggg gaagatccct
     2461 caggaattcc taacaacttg atgccttata ttgcacaagt agcggtaggt aagctatcag
     2521 agctaagtgt gttcggaaat gattatgata cgcttgatgg tactggtgtg cgtgattata
     2581 tccatgtagt agatttagca ataggacata taaaagcttt agaaaaagta tctgaaaaaa
     2641 cagatgttta tatctataac ctaggctcgg gagaaggcac tagtgtatta caacttgtaa
     2701 atatatttga aagtgttaat aagatcccta ttccttataa aatagttcca agacgttcag
     2761 gagacgttgc aacttattat gcaaatgcag acaaagcata taaggaatta aattggagga
     2821 caacaaaatc gattgaagac atgtgtagag atacatggaa ttggcaatca aaaaatccca
     2881 atggctataa ttgatgatac tgtctatatg agtgctgtat tttggaaaaa ttacttaacc
     2941 ttttaatgaa agataaatta aaaaaaatgt ttgacagcgc ttgacaaagg aaacggtttc
     3001 attgtataat gtaaacacaa tgacaccgat gtcaaatata aaaaagaagg tctttatatg
     3061 aaaaacttaa caaaaattaa gttcaaagaa aatggggaat ttaatcattt ccctgggaat
     3121 acagttgtag caaatcttta tactaaacaa gatttgatgg aagttgttga tattattcaa
     3181 tcacgttata gagaattacc atttatcgat aagtttactt taactccaag gaattctatt
     3241 cacatgactg taattgaatt gttgtgccat gaaaatcgtg aaacggagtt ttggagcagt
     3301 aatcttcctc tagatacacc tttacaggaa atacatgatt actttgctaa acaacttgaa
     3361 atttttccat tgttggatga agaaattcat atgcgtataa ctgaaatggg aaaacaaaac
     3421 atactagttg aacctgcaga tgaagcttct gcaaagagat tagaagaaat tcgtacttat
     3481 gtctcagaaa aagcaggtgt ttgtttccct aatcatgata gatatcaatt ccatatttca
     3541 attgggtatc ttcggattcc tctaaccgaa gaggaggaag aagagtttac taaagtcaga
     3601 gcagaattaa ctgaaatttt attagagaag attccaacta ttactgtaaa ccgtattgac
     3661 tatactgtat ttgaagatat gagacaattt gttccatatc acgaaaaatt taaataaaat
     3721 aataaaaaag gagaacttat catgaaaaaa atgaaagttt ggtctactgt acttgcaacg
     3781 ggagttgctc ttactacact tgctgcttgc tctggaggtt caaattctac gactgcttct
     3841 tcatctgaag aaaaagctga taaaagtcaa gaattagtta tctattcgaa ctcagtctca
     3901 aatggtcgtg gtgattggtt aactgctaaa gcaaaagaag ctggttttaa tataaaaatg
     3961 gttgatatcg ctggcgctca attagcagac cgtgttattg ctgagaagaa taatgcagtt
     4021 gcagatatgg tatttggaat tggtgctgtt gactcaaata aaattagaga tcaaaaatta
     4081 ctagtacagt acaagcctaa atggttagat aaaattgatc aatctttatc agataaagat
     4141 aattattata atcctgtgat tgttcaacca ttagttttaa ttggggcgcc tgatgtaaaa
     4201 gaaatgccta aagattggac tgaattaggt agtaagtata aaggtaaata ttcaatttat
     4261 ggtcttcaag gtggtacagg acgggcaatt ctagcaagta tcttagttcg ttacctggat
     4321 gaaaaaggag atttaggcgt atcagaaaaa ggatgggaag ttgcaaaaga atactttgca
     4381 aatgcctata ctcttcaaaa aggtgaaagc tcagttgtta aagcgttaga taaagaaagc
     4441 ccaattcaat acggtatgat ctggggttct ggagcgttaa tcggacaaaa agaacaaaat
     4501 gttgaattca aagttatgac tcctgagatt ggtgtaccat ttgtaactga acaaactatg
     4561 gttttaagca ctagtaaaaa acaagcgtta gctaaagaat ttattgattg gtttggtcaa
     4621 tcagaaattc aagtagaata tagtaaaaaa tttggatcta ttcctgcaaa taaagatgcc
     4681 ctaaaagagt tacctgaaga tacgaaaaaa tttgttgatc aagtgaaacc acaaaatatt
     4741 gactgggaag ctgttggaaa gcatttggat gaatgggtag aaaaagctga attagaatac
     4801 gtaaaataag aaataagagt tattttttat gtccaagtct ctctattatt tctaaattta
     4861 aggaagaaat aaagagaggc ttttggtttt agtaatagag ggagttagtg agatgattaa
     4921 atttgataat attcaaatta aatatggtga ttttgttgca attgataatc tgaatttaga
     4981 tatacatgaa ggggaattct ttacatttct tgggccttca ggatgtggta aatcaactac
     5041 tttgagagca ttggtaggtt ttttagatcc atcatcagga agtattgaag ttaatggaac
     5101 agatgtcact catttggaac ctgaaaagcg tgggattggt attgtatttc aatcttatgc
     5161 gctatttcca actatgactg tttttgataa tattgcattt ggtttaaaag ttaagaaggt
     5221 agctccagat gttattaaag ctaaagtatc agcagtggca gcaaaaatta agatctctga
     5281 tcaacagtta cagcgtaatg tatcagaatt atctgggggt caacaacaaa gggtagcatt
     5341 ggctcgtgct ctggttcttg aacctaaaat tctttgtcta gatgaaccat tgtcaaacct
     5401 tgacgcaaaa ttacgtgtag atttgagaaa agagttgaaa agacttcaaa aagagttagg
     5461 tattactact ttatatgtta ctcatgatca agaggaagcc ttgactttat ctgatagaat
     5521 tgcagtcttt aacaatggat acatcgaaca ggtcggtaca ccagtagaga tttatcataa
     5581 ttctcaaact gaatttgtat gtgattttat tggagatatt aatgttttga ccgatgaaac
     5641 agtccacgaa gtattattga aaaatacaag cgttttctta gaggataaaa aaggatacat
     5701 tcgattagag aaagttcgat tcaatcgtga aactgaacaa gattttattc taaaagggac
     5761 aattattgat gttgagtttt ctggagttac aattcactat acaataaaag tttctgaaag
     5821 tcagattctt aatgtaacaa gtattgatag tcaggctgct attagatctg tcggagaaag
     5881 tgtggaatta tttatcacac catcagacgt tctgcaattt taaggaggtg cagtatgcgt
     5941 cataaattaa atttaaaaga ttggcttatt cgtttagggt taatctggtt cttagtaaca
     6001 tttattattt atccaaactt tgatctagta gtgaatgtat ttgtaaaagg aggagaattt
     6061 tcccttgatg ctgtacatcg tgttctaaaa tctcagaggg cacttcagag tattatgaac
     6121 agttttaagt tagcattttc actcattatt acagttaatg tcgtaggtat tctttgtgtt
     6181 ctatttacag agtactttga tattaaaggt gctaaaattt taaaattagg ttatatgacc
     6241 tctttaattt atggaggagt ggttttagcg actggatata aatttgtcta tggtccttat
     6301 ggattgatta caaaattttt acaaaatgtt atcccttctt tagactctaa ctggtttatt
     6361 gggtatggtg cagtcttatt cattatgaca ttttcaggaa ctgctaatca tacattgttt
     6421 ttaacaaata caattcgaag cgttgactat cacactattg aggctgctcg aaatatggga
     6481 gcaaaaccat ttactgtttt ccgaaaagta gtgttaccaa ccttaattcc aactctattt
     6541 gcacttacta ttatggtttt tcttagtggt ttatctgcag tagcagcacc catgattgtt
     6601 ggtggtaaag aatttcaaac tataaatcca atgattatta catttgcagg gatggggaat
     6661 tctcgtgatt tagctgcctt acttgcaatt attttaggta ttgcaactac aattttgctt
     6721 actatcatga ataagataga aaaaggtgga aattatattt ctatctctaa gactaaagcg
     6781 cctcttaaaa aacaaaaaat tgcgtctaag ccttggaata tcattgctca cattgtagca
     6841 tatggattgt tcacagtttt catgcttcca ctaattttta tagtattata ctcatttaca
     6901 gatccagttg caattcaaac aggtaactta acattatcaa actttacttt agaaaattat
     6961 cgcttattct ttagtaatag tgcggcattc tctccattct tggtcagctt tatttattct
     7021 attattgctg cgacaacagc aacaattctc gcagttgtat ttgctcgtgt tgtcagaaaa
     7081 cataaatctc gttttgattt cttatttgaa tatggtgctc tacttccttg gttactacca
     7141 agtacacttt tagcagtaag tttattattt acttttaatc agccacaatt tcttgttttg
     7201 aatcagattt tggtaggtag tttggtaatt ctacttattg catatatagt tgtaaaaatc
     7261 ccattttctt atagaatggt acgtgctatt ttatttagtg ttgatgatga gatggaagat
     7321 gcagcaagaa gtatgggtgc ttcacctttt tatactatga tgaaggttat cattccattt
     7381 attttaccgg ttgttctctc tgttattgct ttaaacttta actctttatt aactgacttc
     7441 gacttatctg tattccttta ccatccccta gctcaaccat taggtattac gattcgatct
     7501 gcaggtgatg aaacagcaac atctaatgca caagctctgg tatttgttta tacaattgtt
     7561 ctgatgatta tttctggaac ggtattatac ttcacacaaa gaccggggcg taaagtaagg
     7621 aaataatcat gacagccact agtcttgggt tatcaaatat tgaaatagtt gtcaggattg
     7681 ttttatcagt agtcattggt agtataattg gtttagagag agggagcaaa tcccagcctg
     7741 caggcatccg aacttatagt attgtttgtc tagctgcatg tttgattatg atgacgaatg
     7801 aatacgtatc ttataaattt gggacaggag atcctacacg attaggagct caagttatat
     7861 caggtgtggg ttttctaggc gctggaacga ttcttattac agataagaag aaaattacag
     7921 gtctgacaac tgcagcaggc atttgggctt cggcaggaat tggattagct attggagtag
     7981 gtttttatga gggagctctt ttagtagcca tttctgtttg gggtgtgata tccatgttcc
     8041 aaccactaaa aaaatatctg caaaatcgtt ctaaaatgat tgaattgtat atagtagtta
     8101 aatctacaga agcctataat cgtgtattgg tttattgtgc agaaaatggt atcagaatga
     8161 ccgattcaag aacagcgttt ggagatgtta attctgacag aattgaatac tttgatgttc
     8221 cggacaaaaa aatagcgtca tttattactc tggaattatc aggtagattt gaacatcttc
     8281 gactgatgga agaaattgct aatattgtag gtgttattta tgtcgaagaa attagctgaa
     8341 atttagtaaa caaagggaca gatgtttttg tccttttgtt ttttagatgt tttaatcatc
     8401 taatttttga gtagattttt gctcagaggt ttttatgaaa gattataaaa tcaattttga
     8461 tttaggtaaa atagaatatt ttgataataa ttgtttgata caagtataca aatttatttc
     8521 tttttatgat atttgtgaaa tggtgtttgc ctttcattta cctcctgatg agttgataac
     8581 aaatgttatc tttaaag
//
LOCUS       DAVKMU010000064         8291 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKMU010000064 DAVKMU010000000
VERSION     DAVKMU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.064"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            146..793
                     /locus_tag="VW343_001858"
     CDS             146..793
                     /locus_tag="VW343_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9756369.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            924..1337
                     /locus_tag="VW343_001859"
     CDS             924..1337
                     /locus_tag="VW343_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756370.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            1356..1541
                     /locus_tag="VW343_001860"
     CDS             1356..1541
                     /locus_tag="VW343_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756371.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(1808..2251)
                     /locus_tag="VW343_001861"
     CDS             complement(1808..2251)
                     /locus_tag="VW343_001861"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9756372.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(2281..4503)
                     /locus_tag="VW343_001862"
     CDS             complement(2281..4503)
                     /locus_tag="VW343_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9756373.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYPSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5519..6148
                     /locus_tag="VW343_001863"
     CDS             5519..6148
                     /locus_tag="VW343_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9756374.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(6271..8163)
                     /gene="pepO"
                     /locus_tag="VW343_001864"
     CDS             complement(6271..8163)
                     /gene="pepO"
                     /locus_tag="VW343_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9756375.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         2251 a         1904 c         1590 g         2546 t
ORIGIN      
        1 ttattttcag ggttttattt ctatcatttt attttacttt catttggtgg gagaatattt
       61 gaaaaaaaga aaggtagttt aacctatgaa aaaatcacta aaaatttttg ctacatctaa
      121 atagtttgac ctcttcgggg ttgctttggt cgttgggatt gcgattgcat ctggttacct
      181 caactcacac ctcgataaat ttgtagactg gggatcatgg acagctcttg taccctttgg
      241 attgatttcc gtaaccaacg ttgggatttc catgttgtcc actcgtttca cggggaaatt
      301 aagcaaatgg ggaaattact ttggtattgt taataccatt ttgtccggtg ctattgatta
      361 tatccttgga aataaggcgg ccatcattac ctatcccgtc accttcctca tttatacctt
      421 tgctataaag aagtgggaag cttcgcaaga aggcagaccc aaccaaatga gccaaaaaca
      481 ggtaaaattg gcggccatca tcatttccat catcgccttc ctctttgcct ttgtgaccaa
      541 ctatatcgga tatgggggca agatgaatct ccttgcctac gtaacaacta ttgcctttgc
      601 actgtccctc attgccaatg ctttgaatgc attaaaacta acaactcagt ggggcttttg
      661 gttgatttac aatttcgttc agctgacaaa agctggtatt caaggaaact tcgccaatat
      721 cggtaaatac atcttttata tcctcaatgc aatcggagct ttatttgtct ggaatgatga
      781 agaagttaga taatagaagg aaactcaaat aaagaatact agccaactta agagaatctc
      841 ccttctgcta tttttatcat tttagttgta actcccattc ttttggagta gaatgaagtt
      901 agataaaaga gggaggtctt gtcatgaaaa aactctatag aatccatttt atagcaattg
      961 cagttattga tttgctacta tttgcatttt ttatcactag actcgagaca tcttttgagt
     1021 ggctcttgct ctctggttta attttcttcc ttgctcaagg actcctgcta tttctcttgg
     1081 tcgtccgact caaacatcaa ttcgctgaga tttatcctca aatcaataaa aagattcgct
     1141 tctactattt aggggttctc accattgatt ttctattttt tgttctctta gccttcatta
     1201 gttctcagcg tttttcatct cttatgccaa tcatcactgc ttgccattct actttttatt
     1261 atatgacagc tgactaccta agagaaaact atccagactt ttacgacaaa cacatctctt
     1321 tatgggagtg tctctaagga aaaggaggtt ttagcatgaa aaaaatcatc ttcatcaaaa
     1381 ccattcaact ccttgtcatt gatggaatca tgctggcatt tttgacattt aaaagggggc
     1441 ttacttggga ctggattttg atttatagcg gttggctcat tttctttcat cctgtgctat
     1501 tgacctatct ttcaaaccaa ctttgtgacc actttagtta actctattcc cagattagac
     1561 cgagattctg gcgttttgct ttacaaattc tcctatggga tagcctgatg attctctcct
     1621 tggtgtcttt aagtgatatt ccacttttcc ttcagggaac tctcctcatc ctaggacatc
     1681 tcatcccttc ctatcgcatc tgccaaagcc tgaaaagaga cttcccccaa gcatatcaag
     1741 aaccgatttc tttttggagt attttatgat agatgagaaa gaccaagccg actgggcttg
     1801 gtctttctta tctcttttta gtatctagga taatggtaac aggtccatta ttaaccagct
     1861 caacctgcat atctgctcca aagatgcctg tctgaacggg cacttcttgc gctaattttt
     1921 gattgaaagc atcatagaag tctgatgcca tatcaggttt agctgcccct gtaaaggctg
     1981 gacgattgcc tctcttagta tccgcaaaga gggtaaactg agaaatagag aggatttctc
     2041 cttcaatatc tttgacagac aggttcatct tgccttctgc gtctgaaaaa atccgcatat
     2101 tgaccagttt tctcacagca tagtccaaat cttcctcttg gtcctctggt ccaacaccaa
     2161 ccagcaataa aagtccctga ttgatttttc cctgaatctg gccttctata ctcacttggg
     2221 cttttttaac ccgttggata atgattttca taatagcctt tctagtaaga gctaggacaa
     2281 ctagccgttg gtccgtttga cagagtaaac ttctggcaca ctcttaattt tatcgacaac
     2341 cgtggtcagt gtagagaggt tggcaatacc gaaggacaca tggatattag caaacttcat
     2401 atccttggtt ggttgggcat tgaccgttga aatattcttg gttgtatttg aaagaacttg
     2461 cagtacatcg ttcaacagtc ctgtacggtt gagaccgtag atatcgatat gggccatata
     2521 ctccttattt gagctagggt actggtcttc ccattccaca tcaaggagac gttgctcgta
     2581 gttttcttgg gcacgcaggt tcatacagtc cacacggtga atagccacac cacgaccctt
     2641 ggtaatgtag ccaacaatat cgtcaccagg cacggggtta caacacttag caatccgcac
     2701 taggagacca gaagcacctt caataaccac tcccccctca tgcttgacct tgagggtttc
     2761 tttattttca accttgacct cgccaccttt gacaagctcc tctgcctcag ctttggcctt
     2821 ggcacgctct tcctcacggc gttccttttc agtcagacgg ttaaagacgg taatcgcacc
     2881 gatttcccca aaaccaatgg ccgcaaagag ggagtcttct gtcttgtaac tggtcttttg
     2941 cagaacttga tccatgtggc gcttgtccat aaatttattt gccacatagc cattttcttg
     3001 gaactgagcc atcagcatct cacgaccctt gttgacagac aattccttat cttggttttt
     3061 aaagaactgg cgaatcttat tgcgcgcctt gctagtcttg accatattga gccagtcacg
     3121 gctaggtcca aaggagttcg ggttggcgat aatttcaacc tgatcccctg tctttaactt
     3181 ggttgtcagt ggaaccatgc ggccattgac cttggcacca gttgcttttt caccgacctt
     3241 ggtatggatt tcgtaggcaa aatcaatcgg tcctgaatct ttgggaaggg aacggacagc
     3301 tccatctggg gtaaaaacgt aaatctcctc agccaaatag ttttccttaa cagagtccac
     3361 aaattcctta gcatcatcag cctggtcttg gagctccatc atctccttga tccagttcat
     3421 tccaatagct gattccttgc tgttaacttg cccctttata cctttcttat aagcccagtg
     3481 agccgcaacc ccgtactcag ccacctcgtg catttccttg gttcgaatct ggaattcaat
     3541 cggccctttt ggtccataaa cagtcgtatg gatagactga taaccattgg ccttgcggtt
     3601 ggcgatatag tctttgaagc gacctggcat cggtttccaa aattcatgca cgtaaccaag
     3661 catggcataa acatcacttt gggtatctaa aatacaacga atagcaatca gatcatagat
     3721 ttcctcaaac cgttttctct tgtcctgcat tttgcggaaa attgagtaaa tatgcttggg
     3781 acgaccataa atcttccctt tcaagtgacg ttctgtcgta tactcctcta attttgtgac
     3841 tacctcatcc accaaggcct cacgctccct gcgcttttcc ttcatcatat gggtaatctt
     3901 gtaaaactcc gttggattga gataacggaa agacaagtct tctaattccc atttgacact
     3961 ggaaatcccc aaacgatggg caagcggggc atagatttcc atggtttctt tggaaatacg
     4021 ctcctgcttg tcttttcgaa gatgtttcag ggtccgcata ttgtgcaagc ggtcagacag
     4081 tttgaccaaa ataacgcgga tgtcctcaga catggccatg agcatcttgc gatgattttc
     4141 cgctaattgc tcctcgatcg atttgtactc gaccttgcca agcttggtaa ctccgtcaac
     4201 aatcacccgc acatcaggac caaactctct ttccaaatcg tccaaggtcg catctgtatc
     4261 ttccaccaca tcatgcaaga atccacaagc tactgttaca gcatccagct ttagcttagc
     4321 taaaatacct gccacttgga tagggtgaat gatataaggc tcgcctgatt tgcgatattg
     4381 accactgtgg cattcaacag catagaccaa ggccttatgg acaaaatgaa catcctcttc
     4441 cgttaaatat tctttggtta aagcgacaac ttcttcgcct gttaaattca cttctttcgg
     4501 catctctact ctccaattct tcctaccatt ttatcacttt tttaagaata tgaaaactag
     4561 attggaacag aataagaaaa aaataattca aaattgcttg ataattctga attattggcc
     4621 cataatatac tacgaagtta gattttaaac ttaggtgata gaaggagaga tagaagaacg
     4681 gaaaccatat tgtaacccaa agactttctg atttccccaa ttccattgaa gatacgaaag
     4741 ataaacggtg gaactcgtat cacatacact ggtaccttga ctggattttg gaattaatac
     4801 tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag gttgctcaaa gcactgcttt
     4861 gaggttgtag ataagactga caaagtcagt cacatatata cggcaaggcg acgttgacgc
     4921 ggtttgaaga gattgttgac gcggtttgaa gagatttttg aagagtataa aaatcctcaa
     4981 gatactttct tctatccttt agtttataag gagaatacct atgaaaaaaa ctgctatttc
     5041 tatctttgct ctcctaatgt taggagtttg ctgcctgttc ctattcagcc agcaaagcta
     5101 taaaaaactc gttcaatact atgctaacga ccagaacctg cccagtagga taacttatag
     5161 tgaatatagc gacaaatgat aagccaacta cggtagcact ctaaacatca cgtctatcaa
     5221 acaagctaat gacggagttt atgcaaccta tgaagggcaa ttgacacctt tccaatattg
     5281 ataaattgat aaccagcctg tcttcatcta gtcatgctgg tttttaagtt cattttaaat
     5341 ccttacctat tctccctaac tgtgctatac ttaatttata ctcaatgaaa atcaaagagc
     5401 aaactagaaa gctagccgca ggctgttcaa agcactgctt tgaggttgca gataaagttg
     5461 acgcggtttg aagagatttt cgaagagtat tagtacattc tttgagattg gagctagtat
     5521 gaaaatccat aaaaccgtga atcctgttgc ctatgaaaat acctattatc tagaaggcga
     5581 aaagcacctc atcgtcgtcg atcctggtag tcattgggaa gccattcgtc agacaatcga
     5641 gaagatcaac aaaccgatct gtgctattct cttgacccac gcccattatg accatatcat
     5701 gagtctggac ttggttcgcg agacgtttgg caatcctcct gtctatatcg cagagagcga
     5761 agccagctgg ctctacactc ctgtcgataa tctctccggt ctccctcgcc acgatgatat
     5821 ggcagatgtg gtcacaaaac ctgcagaaca cacctttgtc tttcacgaag aataccaact
     5881 agaggaattt cgttttaagg ttctaccgac cccagggcac tctatcggtg gtgtttccct
     5941 agtctttcct gatgctcacc tagtcttgac gggagatgct ctattccgcg aaactatcgg
     6001 acggaccgac cttccgactg gtagcatgga gcaactcctt catagtatcc agacccaact
     6061 cttcacccta ccaaactacg atgtctatcc aggacatggt ccagctacta ctatcgctca
     6121 cgaaaaggcc ttcaatccct ttttctagca agatgatgac aatcgaaatt taagtaaact
     6181 atccagcaaa tctttctatt acaaaaggca tcctatcaag gttttcacac gtgattggat
     6241 gccttttttc tgatgactag attttttgta ttaccaaata atcacgcgct cctctggtga
     6301 acgccacatt ccgtctcctt ctttgacatc ataggttgta aagaaatcgt cgaagtttgg
     6361 tacttgcaca ttgacacgga gtttggctgg tgcgtgcaca tcgacgctag ccaaaagttt
     6421 cataaattct ggtcgacctt tcatgcgcca gatgcgaccg aagttgtaga agaactcttc
     6481 tgctgagaag tctgcttctc tcttagctgc ttcaagcgct gctgcgattc ctcccaagtc
     6541 agccacgttt tctgatacag tcaatttacc gttaatggtt gctccataag aatcctgtcc
     6601 atcaaattgg tcaatgactt tttgtgtttt ctccttgaag gcagcatagt cgctctctgt
     6661 ccaccaatcc ttgaggctac cattttcgtc aaaggaagcc ccgttagtat caaaggcgtg
     6721 ggaaatttca tgggcaatca ctgccccaat accaccgtag ttagcagaag atgactgatg
     6781 caagtcatag aaaggcgcct gtaaaatggc cgctggaaag acaatcaggt tcttctgagg
     6841 attgtagtag gcattgacca tatgagcagg catgccccat tccttatagt ctacaggctg
     6901 gttccactta ctccaactgt gcttgatttc cacacgcgca aaggctagag cattctcaaa
     6961 aagactggca gtttcattca ctaccttatc cttgtaacgt gcaggcaatt cttctggata
     7021 gccaatataa ggtttgatca cattgagctt cacgatagcc tgtttacagg tttctggagt
     7081 gagccagtca ttcttaagca gacgctcctt atagacatca atcatggttg ccactttttt
     7141 ctccacatcc gccttggctt ctggagagaa cttctcacgg gcgtaccaaa gacccagggc
     7201 ttgcttgaaa ggttcttgtg ctagatgata agctgctttg accttatctt ttgcctctgg
     7261 aactccagaa agggcacggc tgtaggcacc agacaaaaca cggatatcct ctgttaaata
     7321 gctggttgaa agattgacaa cactcaaaat caaggttgct ttaaggagag accaggcttc
     7381 ctcactgtag aattgctctg ctgcttgcca gaaacgttcc tcgtctacaa taaccttgtc
     7441 tggtaattgc ccaataactg ctttgaagaa gtcatccaaa ggtagggcag gcgcgaattt
     7501 cttaaaatct tcgtaagaat atggatgata gagtttagca tattctgaac tttcttcatt
     7561 agagagcacc actgccgcaa ctcggcggtc caattcaagt cttttttcta gcaagtcttc
     7621 aatttcttca tcagagaaat cataagcctt gaggagattt gcgctgcttt ctttccaaag
     7681 agtcaagagc tcttcgcgct gaggatgttc ttctgcatag taggtcgtat ctggcaagat
     7741 tgtgcttgga gcgctagccc atagaacatt gattctagca tccataaagt ctggcgatac
     7801 accaaaagga aggaagtttg gttttcctgc aagctcaaac tctgctagtt tagctgtaaa
     7861 atccgcaaaa gtttccaatt cttggaattc tttaaggagt ggtaagacag gtgtgatacc
     7921 gtcagcttct ctcttgtcaa aatcacgaac taggcggtgg tatttgacaa agttttccaa
     7981 gatagcatcc tcaggcactt cttcacctgc taaccacttg tctgttgtcg ccagcatcag
     8041 gtcttcaatt tcctggtcta aatcaacaaa acctccagtt tgagacttat ctgctgggat
     8101 ttcagctgtc tgttgccatt ctccattgat agcatcataa aaatcatctt gataacgtgt
     8161 catcttgttc tcgctttcat ttgtatttgc atttatctta acaaaaatca ctagggaatg
     8221 caattaataa ccatttaact attatttttt agctattata aaaaatcttt ccatgataaa
     8281 acgcataatt c
//
LOCUS       DAVKMU010000065         8282 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKMU010000065 DAVKMU010000000
VERSION     DAVKMU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.065"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            137..1753
                     /locus_tag="VW343_001865"
     CDS             137..1753
                     /locus_tag="VW343_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756376.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTSQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            2014..2442
                     /locus_tag="VW343_001866"
     CDS             2014..2442
                     /locus_tag="VW343_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756377.1"
                     /translation="MSEIVEMSKKISNLNDKKEFHKKNCELRFQDSVLDNINSVPGTT
                     HLFSCLNIQTLVKIILYIFRYNSCMKIFNFRDKELIFFNKIVNGLIQNIEENLEDDIE
                     RILKYLYICLFNEIFIIKNKVNFFDDVEFNQTLSEFLDKL"
     gene            2454..3203
                     /locus_tag="VW343_001867"
     CDS             2454..3203
                     /locus_tag="VW343_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9756378.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            3216..4388
                     /locus_tag="VW343_001868"
     CDS             3216..4388
                     /locus_tag="VW343_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU9756379.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKSILP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIAIPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     FFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFEFGGYCLPKDTKQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            4783..5646
                     /locus_tag="VW343_001869"
     CDS             4783..5646
                     /locus_tag="VW343_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9756380.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFLYIPIFE
                     AELESMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFTQSLQVLENPQLTSLFE
                     LRLQQYKKLID"
     gene            5932..6129
                     /locus_tag="VW343_001870"
     CDS             5932..6129
                     /locus_tag="VW343_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9756381.1"
                     /translation="MLNLQFAETMELTEAELETGYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            6197..6862
                     /locus_tag="VW343_001871"
     CDS             6197..6862
                     /locus_tag="VW343_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9756382.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            6934..8160
                     /locus_tag="VW343_001872"
     CDS             6934..8160
                     /locus_tag="VW343_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9756383.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLT
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         2685 a         1191 c         1532 g         2874 t
ORIGIN      
        1 aatcaagaag gcaagagaag aaaaaatttt taacgaattt ctttttttga aatacatgtt
       61 attatattat tttggttttt tctattgtat atatgacaac tatgttcaat taaaatttag
      121 aaagtgagaa actattgtgt atactattat aaaatcaaat ataaaaaaat ttagtttatt
      181 aacgatattt attgttgctg gtcaattatt gctaatttat gcagcaacta ttaatgctct
      241 ggtgttgaat gaattaattg cgatgaattt agagcggttt ttgaaattgt caatctacca
      301 aatgattgtc tggtgtggga taatattcct tgactgggta gtgaaaaatt atcaggttga
      361 agtgatccaa gagtttaatc tagagattcg aaatagagtt gccacagaca tctctaactc
      421 tacctatcaa gaatttcata gtaaatcatc aggaacatat ctttcgtggc taaataatga
      481 tgttcagact ttaaatgatc aggcttttaa acaacttttt ttagtaataa aaggaatttc
      541 tggtactata tttgcagttg tgactcttaa tcactatcat tggtcattga ctgtagccac
      601 cttgttttca ttaatgatta tgctacttgt accaaaaatc tttgcatcga aaatgcgaga
      661 agttagtcta aatttaacta gccaaaatga agctttttta aaatctagtg agactatatt
      721 gaatggattt gatgtgttag cgtccttgaa tcttttatat gtattgccta agaaaattaa
      781 agaagcagga attttattaa agatggttat acaaagaaag acaactgtag aaacgttagc
      841 aggcgctatt agcttctttc tcaatatttt ttttcagata tctctcgttt ttttaacagg
      901 ctatcttgca ataaaaggaa tagtgaaaat tggtactatt gaagcaatag gagcactaac
      961 aggtgttatt tttacagcgc taggtgaatt aggaggtcaa ttatcctcta ttattggtac
     1021 gaagcctatt tttttaaaat tgtattcaat taatccaatt gagtcaaata aaatgaatga
     1081 tatcgaacca aatgaggtga atagagattt tccgttatat gaagcaaaaa atatttgcta
     1141 taagtatgga gataaagaaa tattaaaaaa cttaaatttt tgttttcaac gtaatgaaaa
     1201 gtatttaatt ttaggtgaaa gtggaagcgg gaaatctaca ttattaaaat tattgaatgg
     1261 ctttttgaga gattatagtg gagaattgcg attctgcggg gatgatataa aaaaaacctc
     1321 ctatttaaat atggtttcga atgttctata tgtagatcaa aaagcttatt tgtttgaagg
     1381 tacgattaga gataatattt tattggaaga aaattatact gatgaagaaa tactacagtc
     1441 tttagaacaa gttggtttga gtgtaaaaga ttttcctaat aacattttag attattatgt
     1501 tggtgatgat gggagattac tgtcaggagg gcagaaacaa aaaattactt tagctagagg
     1561 cctaattaga aataagaaaa tagtattaat tgacgaggga acttctgcta tcgataggag
     1621 aacttcgtta gcgattgaac gtaagatatt agatagagag gatttgactg tcattattgt
     1681 tacccatgct ccgcatccgg aacttaaaca atattttact aagatatatc aatttccaaa
     1741 ggattttatt taagaatata ggggagggaa acatgagatt ctattgtgaa gcttatgagg
     1801 taattactga agagaaaaaa gtatcatttt tttatgtaga tgagaaaccc ttaaggtatc
     1861 tgttatttga ctatatattt ctactagttc tagctaaaaa aaaatatccc aatcttatga
     1921 actgcacccc aaaagttaga tttattctgt ctaacttttg gggtgcagtt cattttgcat
     1981 agttttggca ttcatcctag atatccatat aaaatgtctg agattgttga aatgtctaaa
     2041 aaaatatcaa atctaaacga taaaaaagaa tttcacaaaa agaactgtga gttgaggttt
     2101 caagatagtg ttcttgataa cataaatagt gtcccgggta caactcattt atttagttgt
     2161 ttaaatattc aaacattagt aaaaattatt ttatatattt ttcgatacaa cagttgtatg
     2221 aaaatattta attttagaga taaagagctt atctttttta ataagattgt aaatgggctg
     2281 atacaaaata ttgaggagaa tttagaggat gatattgaaa ggatattgaa atatttatat
     2341 atatgtttat ttaacgaaat attcattatt aagaataagg tgaatttttt tgatgatgtg
     2401 gaatttaatc agacacttag tgagttttta gataaattgt agagggaatg gttatgagta
     2461 acaatactaa gtatatcttt ttatctccac atttagatga cgcaattttt tcctgtggtg
     2521 attatatttc aaaattaact tcagaaggag aaattgtact tgttattact atattttctg
     2581 gttacccttt gagccaacag ctacagccct ctgctaagca atttcataag ttgtgtaacc
     2641 ttgggaaata tccgattgaa gaaaggaaga aagaagacag gttagcttgt gaacgtctac
     2701 aatgtgattt tagacactta tcgtactatg aatgtctcta taggaaagat agaaatggca
     2761 attttctata ccgtcatatc tatagtgaat taaaaaatga ggacacatta aagaatgata
     2821 tcataaaaga gctgttaatg catttagacg ataaatgtgt cgtatattgt ccgctttcac
     2881 ttggtgacca tgttgatcat gtttttgtga atagtattgg aagggcgctt gaatttatgc
     2941 gttataaagt aatatattat gaggacttcc catatgtaag tgatagtagt atggttagtt
     3001 atatgggaaa aactaaagaa ttaaaaatgt atcaagagga acttgatgaa aaacattata
     3061 ttgatagaat atcatcaata ttgtgctata aatctcagat tttgataatc tggaaatcag
     3121 ttgaaaaact tttaaacaat attaaagagt tgtatttaag gaatggggca gcttattcta
     3181 ttagattttg gattaagaaa taaggaagta gttttatgaa tatagcagta attggtttgg
     3241 ggcatgttgg gctggcctac gcgttactat ttgcatctaa atataaagtt gttgcatatg
     3301 atatagattc tgtaaaaata aataatttaa aaaagagcat tcttccatct aaaaatgaag
     3361 agcttatgaa gtttttttgc gagaataact taaatattac tttttttgat acattttctg
     3421 aaattaaaaa taatattgat tattatatta ttgcgattcc gacagattat gatgagaaaa
     3481 ttggtagttt taatacatat gaaatcgaac aaacggtatc gaagattctg agggtaaaac
     3541 ctaatggaaa gattatttta aagtcaacag ttccgatcgg cttttcaaac aaattaaaaa
     3601 ggctgtttga tacaaaaaat atcatttttg tccctgaatt tttgagagaa ggttgttcta
     3661 tatatgataa tttatatcca agtcgcatag ttgttggaga tgagacagtt gaaggaagaa
     3721 aaattgcaga gttgttcctt tcgattagta ctcatagtac tgccaatatt aaaaatgtta
     3781 tgttagtttc tcctactgaa gcagaagcaa ttaagctttt ttctaacaca ttcttagctc
     3841 tccgtgttgc tttttttaat gaactagatt tttttgctga gaggagaagt ttaaatgctg
     3901 aagttgtaat aaaaggtgtt tgtttagatc caagaattgg aaatttttat aataatcctt
     3961 cttttgaatt tggaggatat tgtcttccca aagatactaa acaattaaaa aaagaattta
     4021 tagaaataaa tgccccagtg atagaagcga ttgatatttc aaacacaaat agaaaacagt
     4081 tcatagttaa acaaatattg gaacgaaagc caaagatagt aggaatatat aaattgggga
     4141 tgaaatataa ttcagataat tacaaagagt cagctatttt aagtataatt aatgaactat
     4201 tgattgtcgg tataaaaatt ttagtatatg aaccaaattt aaacgttagt atagataacg
     4261 taatttttga aaagaatttt gaactattta caaaacaaag tgatctaatt gttgcaaata
     4321 gatgggatag gggccttgaa gcttataaag ataaagttta cacaaggggg atctggataa
     4381 gagattaact attgatatta aaaaggcaat aatttataaa atttgtacaa aaaaatgtta
     4441 aattttcaca ttttcatagt atactgttta atagaaagta ggttctttgt aaccgctcca
     4501 ttataaaatc ctttcactct tccggtatgc tattaggaaa aacataattg gaggatattt
     4561 tgagctcaca cctcatcgtt ccagtggctg ttccccaagc tcgtgtactg cccaaaaaag
     4621 ttagacattc tatttagaac gaggattgag ttctgtattg tacaaggctc ggtcctttta
     4681 gagtcagctt aaggctggct ttttcaatca ccaaagtgtc agaatgtttt gacaaatgaa
     4741 cacaaataat gatataatat gcaaaagcta ggaggtggta ggatgattga aaaaatggaa
     4801 ctgggggaat tttacaagga attacgtttg gcgagaaagc tcaagcagtc agatgtggct
     4861 tgtgacgggc taacagcttc tcagttgtcc aagtttgagc tagggcagtc catgttgtct
     4921 gctgataagc tcatattagc catccaaggg atcaatgtga cctttgatga gtttgggcac
     4981 aagctcaaca actatcaaga atctccacat atgcagattg gtagaaaggt tgtggatcgc
     5041 tttgctcatc aagatatagc tggcttagag caactgttgg aggaagtcga gcaagaacag
     5101 atggcagaga cttatcgtcg tttgaatgct attgtaatca aagatgccat ccactcacta
     5161 gataaaagct acccactagc agaggaggat agcgagtttt tgaccaccta tctctatgct
     5221 atcgagtctt ggacctggtt tgaactctat ctcttttgca atactctgcc cttcttgagc
     5281 aatcaagatc tgatcttttt atcaacctcc ttactcgaaa aatccaaaga atttaaagag
     5341 ttagtacaca atcgattgta tatgaagcaa ggactcttaa atatcttatc agaactcatg
     5401 gagcgaaaac ttttcttata catcccaatc ttcgaagccg agttggagag catgttgcgt
     5461 ccgtacgatg tttttgaaaa agtatcgtgg caatttttaa agaagatgag tgtctttctt
     5521 caaacaaagg gaagcaatca aaaagagatt gaacgcttta cccaatctct gcaggtatta
     5581 gaaaatccac aattaacatc cctttttgaa ttgcgtttgc agcaatacaa aaagcttatc
     5641 gattagttag aaatggcaaa ataataataa tctcagcaac gatcgtcaat aatgttaaaa
     5701 cttgtttctt catattactt ccctcctcat tagtaataac tttattatac cagggaaata
     5761 atcaaatcta ccaaaatcgc aaatctgaaa tttctataag aaaaaatatc aaatatgcga
     5821 ttttctaaaa taagccaatt tccgtgttat actgtccttg taaaacattt gaagcaaatc
     5881 ctaggtcgca gaagtggttt acaaatgaag ataattgaag gagtgtaaaa gatgttaaat
     5941 ttacaatttg cagaaacaat ggaattgaca gaagctgagt tggagacagg ttatggaggg
     6001 gaatttggga ataatgctgt tatcccagcc ggtgcttggg gaggtttagg aacgtcttgg
     6061 tcaatcacta atttctggaa gaaatatttt aaccatgatt cttccactgt taatcgtcgc
     6121 cattattgat attcagagtc cttgcctttt tcttgggtag ggactgtctt tatttcatag
     6181 tataggagaa aatagaatga aaaagataat ctcacaccgc tactttatta tagtttttct
     6241 actagttatt gctgaccaga agttcagtgt tttagtttta cgcagcgacc ttgttgctgg
     6301 gctatctgac tttgcctatt atctgtcgga tatgatgttg aattttcttg tggttttatt
     6361 tgctcttatt gctatgattt ggtcggggaa atggcagaaa atcaatagta gaaagtttaa
     6421 aggatcctat cttttctatt ccttcctagc tctgcttgct tttgttgctt ggaattttgt
     6481 cacctttttt cttttcccac ctactcgaaa tgaaatttct tatcaacatg ctgctcctac
     6541 ttttacagga gctacggcat ttttgatgta ttttttttat cctgtgattg caggtcccat
     6601 ttttgaagac atgatttatc gtggattagt gatgactgct ctggaaaaag gaaagaaatg
     6661 ggggctagat gtacttggtt ccgctgtttt atttggggtc tcgcacatta gtaatcacgg
     6721 ttgggtcttg acagactttg tcttctatat gggtggaggt cttatatttg cagtcttatt
     6781 tagaatgaca aagtcaattt attggcctat tggactgcat atagtctaca atggcattgg
     6841 tcagcttttg atgttactgt aattttggtt gttagtagta tcgcggtcta tccttgggga
     6901 aagattccca tttgaactta agaaggagtt gtgatgaaag tatttcttca aaatagagat
     6961 tttaggcaat taactatcaa ccagtggatt tcaacgcttg gggatacgat tttttatctg
     7021 gcctttctga attatgtggc agatgcgtct tttgcacctt tgacgatttt actcattacg
     7081 atttcagaaa cccttcccca aattttacaa atctttctgg gagttttggc agattttcaa
     7141 catcatcgtg tcttgaaata cacagtcatt agttttgcaa aatttttgct ttactctatc
     7201 gtttctttat cactttcagg gcagtctttc tccttgttgc tagtagcatt tatttgtctg
     7261 cttaacctct tgtctgacac attgagttac ttttcaggag ccatgctcac tccgattttc
     7321 attagaatta ttgggcaaga ccatctggca gaagctattg gctttaaaca gtcaactgtt
     7381 agtttagtga aaacaatcag taatatccta ggaggagtct tactaggcat tctatccatc
     7441 cagtttattt ccttactgaa cgctcttacc tttttaatcg catttttagg tatcctcttc
     7501 ataaaaactg acctcttgaa agtagaaaaa acgattagct atcaagaagg actctctgta
     7561 aaatcctttt gccagcattt gctccaatca tcaaaattga tatggaatat gaataaggtg
     7621 ctcttggttt tgtttattat ctctactagt caagcagtga taaatgttac ggttcctatt
     7681 tccactctat ttttaaggaa ccagcccttt ttgaatttac aaacaggtca atctcttgcc
     7741 ttgctatcca cttttgaact gtcagccctg attgtcggaa gccttgtaag tggctatctg
     7801 caacatacca tttccataaa aacagctctc tatgcctcac ttgtcatcca gttgcttctt
     7861 ctagtaggat ttgccacagt tcgttttgac tggattctca tctttagtac cttggatgcc
     7921 tttttcgcag gtgttctctc tcctagattg caagaactcg tttttaaaca aatacctgag
     7981 gagtcaatgg gagcagttca atcctctatc ggtgccatta cggttgtttt acctagctta
     8041 tttacaatag ctttggtaac cattgctact agctttggaa ctctggcagt tagctttgtt
     8101 ttattgctat ttcttctagt tgcctttgtt atgcttctga atattcgtga aagtatttag
     8161 tggagaattt ctataatata tgtttgagac tggattgcca gtctttttat gatggtcaaa
     8221 atcaacagac tttttctctt ttctgaatgt gttatagtag actgaatcta aaatagtacg
     8281 aa
//
LOCUS       DAVKMU010000066         8083 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKMU010000066 DAVKMU010000000
VERSION     DAVKMU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.066"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(91..963)
                     /locus_tag="VW343_001873"
     CDS             complement(91..963)
                     /locus_tag="VW343_001873"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9756384.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            1199..2509
                     /locus_tag="VW343_001874"
     CDS             1199..2509
                     /locus_tag="VW343_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9756385.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALKLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            complement(2875..3069)
                     /locus_tag="VW343_001875"
     CDS             complement(2875..3069)
                     /locus_tag="VW343_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9756386.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(3528..4256)
                     /locus_tag="VW343_001876"
     CDS             complement(3528..4256)
                     /locus_tag="VW343_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9756387.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            4414..5823
                     /locus_tag="VW343_001877"
     CDS             4414..5823
                     /locus_tag="VW343_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9756388.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            5841..7136
                     /locus_tag="VW343_001878"
     CDS             5841..7136
                     /locus_tag="VW343_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9756389.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            7141..7449
                     /locus_tag="VW343_001879"
     CDS             7141..7449
                     /locus_tag="VW343_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9756390.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            7446..7754
                     /locus_tag="VW343_001880"
     CDS             7446..7754
                     /locus_tag="VW343_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9756391.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEVHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         2619 a         1541 c         1423 g         2500 t
ORIGIN      
        1 ttactatatt tgaagaggcg tttaaactat ctgacataaa actcgttcta gaggaaagac
       61 atcctttaaa aagttagttt attttacaac ttagacatca aggtaggtta accccttcat
      121 ggaaaaatca agactcttag cactatgggt taaactacca ctggagacgt aatcaatcgc
      181 taaaccacga aaacggctaa tagtggtcat atcaatattt ccagaacatt caatccgaga
      241 acgtcctgca attagggtaa tggcctgttc aatctgttcc aatgacatat tatccaacat
      301 gataatatca gcacccgccg ccgcagcttc ttcggcagca gcaaggcttt ccacttccac
      361 ctcgaccatt ttcacaaaag gggcataggc acgcgcttga gcaattgcct tttgaacact
      421 acctactgcc gcaatgtgat tgtcttttag catgatagca tctgataaat taaagcgatg
      481 attatagcca ccgccaactc tcacggcata tttctcaaaa agacgtaaat taggagtagt
      541 ttttcgagta tcaaatacct taatgcaatc atcgcctaag gcttctacat aagcagctgt
      601 catcgaagca atccctgata aatgttgtaa aaaattcaag gcaacgcgtt cacatgttaa
      661 gagacttctc accgagccta tgatttctaa aaccaaatcg ccactagtca aacgatcccc
      721 atccttaaat tgatgaggat tctggaaggt cacctcggca tcaaataggg taaaaaccct
      781 ttgaaaaacg gttagccccg ctaaaacacc agcttccttg gcaaaaagcg acaccttagc
      841 ttggccatga tgatcaaaaa tggcattggt actgtaatct tcggaatgaa catcttctcg
      901 caaggctgct ttcaatgtat catctatttg aaaaggggtt aaatcagttg aaatgattga
      961 catcactaag tcctctattt ttaatatcac ttgtaagcac tagaggcaat tactattaac
     1021 ttccatagga aatcacaagc ctgaatacct catttttaat tctaacaagt gtcaatacaa
     1081 ttgtcaagac aattgtattt ttttgtgtat aatagtcaag taccaatagg aaacaggtgg
     1141 tcttttgagc gccaaaattg ccaacctatt tccatattct aactgaaagg acaattcaat
     1201 gattcattta ataatgatta gcgccattgc tctagccatt ggaattggtt accgcaccaa
     1261 aatcaatatt ggcctgctgg ctattgcttt ttcttacctc atcgcaacca ctctcatggg
     1321 attaagcccc aaagaacttc ttcatttttg gccaacctca ctctttttta ccatttttag
     1381 cgtctctctc ttttataacg ttgcaacaac taacggtact cttgatgttt tggctcaaca
     1441 cattctctac cgcacacgca cccaccctaa cgccctctac atgattttat acctgatggc
     1501 aacccttttg tctgctttag gtgctggatt tttcactact atggccgttt gctgtcctct
     1561 agcgattacc ctctgtcaaa aagcggacaa acaccctttg attggagctc aagccgtcaa
     1621 ttggggagct tcaggagggg ctaatttgat aaccagtagc tcaggcattg tctttcaagg
     1681 cctgtttaag caaatgggat gggaagagca agctttttca cttggcaatc atatctttat
     1741 cgtcagcatt atctacccac tcatcgtctt gcttttatta tcctgttaca gtcactatag
     1801 taagggaaga acaaactcat ccttgactat tgaccaacca ccactcctca gcaaggttca
     1861 aagacaaacc accttgctca tgatcagcag catggttttg gtttggcttt ttcccttgtt
     1921 gcatctcatt ttcccaaaca tcgcctggat agctacttat caaaaaacat ttgatattgg
     1981 ctttgtctca atactcatgg tctgcctcgc cttaaagctc aaattaggga agcaagaagc
     2041 cattttagct aaggttccct gggctacaat tatcatgctc tgcggcatga gcttactaat
     2101 gtctctagcg gtcaagtcag gtttagtcac actgataggg cacctcatga caacaaccat
     2161 tcctcatttc tggttacctc tgttcttttg tgtgatagcc ggagtgatgt cactctttag
     2221 ttccacccta tccgttgtag ctccagccct ctttcccatc attgctatca tcagtgctca
     2281 aaatcctcag attgatattc acttactcac aacagccacc gttattggcg ctctatctac
     2341 taatatctca cccttttcat cagctggttc tctcattcaa ctctcacttc ccaatataga
     2401 ggaacgcggt cttgccttca aaaagcaaat tattctgggt gtccctatca gcttaagttt
     2461 gggactcttg accacctgga ttctcatcct tctagcttcc ttaagttaga ttcattatct
     2521 aacttaaggg tggacttggt ataataaagt agacacaaaa ttaagtggaa acgaggaaaa
     2581 gtcattatgc caagaaaaac ctttgataaa gccttcaaac tctctgctgt aaacctcatc
     2641 ctgtaaaaat aattagttca actttaaaaa ttcatcccaa tagtctttat cgatggattc
     2701 aagaatatga aaaatatgaa gaaagtgcgt tcccaggaca tgggagcgca cttcgtcata
     2761 ctcaatttga gatgtagtga aatgaaataa gaacaggaca aatagatcag gatagtcaaa
     2821 tcgatttcta acaatatttt agaagtagat gtgtactatt ctagtttcaa tctactatag
     2881 taaagttgac tttcctgtac catttttacc agtaattaat gtcacgcctg aactcgaagt
     2941 atcaaaagaa atattatcaa acaatatacg atgatccacc ctatacgaaa tattttctac
     3001 tttcattttt tctccttttg aactaatagc aaaagaaacc gttctgtttt catactcttt
     3061 ccccttcatc tatattattc gtaatgaaaa ataaaaaata aaattttttt aaatgattat
     3121 gattcagaat ttatttaata tattaaaaga agatatgaac aaataaattg ggagattgaa
     3181 attcatttct agcaatttgt tagaagtcac agtctactat tctaacatca gtatactata
     3241 tgtgttacaa gaaaagactc aaaaacttct cgtcagtgta agaatatttg aaacactatt
     3301 taaaactaaa taactataag aagtagatca ggtgaacaat ttcagtacct actctataag
     3361 ctatacgaag atatttttca acatgatgga taaaacgatc actcttttaa agccatctta
     3421 tttagaaaag agaaaccatt taagcataga tgggaccttc ttgccctgtt ttaaattttt
     3481 cttaaactag agtctgtaaa agattggctt cattttcaca ttttctccta ggaggaatgt
     3541 cgtaatgcaa aagaattaaa ttcaaataaa tcatagcgat gacgactaat cgagtactca
     3601 aacggagttc cgttatccaa ataagccact tgttctactt caaataccgg ttccgttgga
     3661 aggagctgca agtaatgttg ctcattttct gaactagaag aagctcgcaa gatttttgta
     3721 gcactggcaa tatgcaatcc taactttcct tcaatgtaat tgtaaatcga tttttgtaaa
     3781 atatcttcag taatacctgg aataacatca gtactcatat aagtttgttc cagaacataa
     3841 ggtttaccgt caataatacg cagacggtaa atattataaa ctggactctg caaagcgacc
     3901 tgtagttttt ctgctaaaaa ttcatttgca aattctaatt tgaagtgaat aatcctcgag
     3961 tcaattgtag aatgagcatc ttcagatatt tttgtcagtc cccggatatc tctttctgga
     4021 acgattaatt ttctttttga gctgccacga gagagaacaa aggttccctg cccacgtttt
     4081 ctaaaaatta agccctcaga aactaacaag tctaaagctt ttttgatcgt cattcgacta
     4141 caggcaaact gtttagctaa tgtcatttca tcaggcaatt gttcgttcat ggtatattta
     4201 ttacaaagaa tcttttcacg aatctcattg taaataaata aatattttgt actcatttaa
     4261 tctggttctc tctataatat tctaatagta gtataacaaa gttcaagaaa tgaggcaaac
     4321 acaagatatg tatcaacaat ttttaaaaat gtctagacaa attttaaaat tatgctatac
     4381 tttatgtata gaataataga aaggaggttg agcatgaaaa aaaagttagt atttcctaat
     4441 ctattttggt ggggagctgc ttctagcgga cctcagacag aaggtcaata tggaaaagta
     4501 catgaaaatg tgatggacta ctggttcaaa acgcatccag aagatttttt cgataatgtc
     4561 ggacctcttg tagccagtaa cttttttcat acttacaccg aagatttcca cttgatgaag
     4621 gaaattggag ttaattcttt ccgcacttcc atccaatgga gtcgactcat caagaattta
     4681 gagacaggtg agcctgatcc aaaaggtatt gctttctaca atgccatcat tgaagaagct
     4741 aaaaagaacc agatggatct tgtgatgaat ttacatcatt ttgatttacc agtggaactt
     4801 cttcaaaaat acggtggttg ggaaagcaaa catgtagtgg agttattcgt gaagtttgcc
     4861 aagactgctt tcacatgctt tggagataag gttcattact ggacaacttt caatgagcca
     4921 atggtcattc cagaagcagg atacttatat gctttccatt atccaaatct aaaaggaaag
     4981 ggaaaagagg ccgtacaagt catctataat ctaaaccttg ctagtgcaaa agtgattcaa
     5041 ctatatcgct cattagaact tgatggaaag attgggatta ttttaaactt gacacctgct
     5101 tatccaagaa gtaattctcc agaagactta gaagcaagtc gatttacaga tgacttcttt
     5161 aacaaagtct tcttgaatcc agctgttaaa ggaactttcc cagaaagatt ggtaaaacag
     5221 ctagagagag atggcgtgtt atggagtcat accgaaaaag agcttcaact gatgaaatca
     5281 aatacggttg attttcttgg agtaaactac taccatccaa aacgtgttca agcacaagca
     5341 aatcctgaag aatatcagac gccctggatg ccagaccaat acttcaaaga gtatgaatgg
     5401 ctggagcgtc gcatgaatcc atatcgtggt tgggaaattt ttccgaaagc catttatgat
     5461 attgctatga ttgtgaagga agaatatggt aatatcccat ggtttatcag tgaaaacgga
     5521 atgggtgttg aaaacgaagc acggtttatc gatgaaaatg gagttatcga tgacgtgtat
     5581 cgtattgaat tttatgaaga acatttaaga tggctacata aagccattga agagggaagt
     5641 cactgttttg gataccacgc ttggaccgca tttgattgct ggtcttggaa taatgcatat
     5701 aagaatcgtt acggatttat ctccgttgat ttagaaacgc aaaagagaac catcaagagc
     5761 tcaggaagat ggtatcgcaa agtaagtgac aataacggtt ttgaagtaga aattgaggag
     5821 taaagagagg agatattcat atgaaaaact caaaatttat agaccaattt gccacctttg
     5881 ctggtaaact agggaaccaa attcatttaa aaaccctaag agatgcattc gtaacagtaa
     5941 tgccattata tattttggca ggtttaatcg ttcttttgaa caacacggta tttaagtgga
     6001 ttttccaagg ggatacatta acaagattcc aatattgggg aataacaatt gcaaacggta
     6061 ctttaagtat ttcaggtatg attattgctg taatggttgg ttatttctta gctaaaaaca
     6121 gagatttcga aaacccgtta gcagcatcaa tgctatcatt agtttcttta attgtgatga
     6181 tgccaaatac agtttctgta gttcctgacg gagcaaaaga tgcggtaaac atttcaggtg
     6241 ttctttcatt caacaacaca ggtacaggcg caatgttcgc cggggttatc gtagcgatta
     6301 ttgcaacaga attattcatt gaattatcaa acgttaaagc tttacaaatg aaccttggtg
     6361 aaaatattcc accagctgtt agtagatcat ttagcgtatt acttccagtc atgaccgtca
     6421 tctccttatt tggggttgtt tcagcattat tattcaatat aactggaatg aacttaatct
     6481 caatcattac aatctttatt caagaaccaa ttcgtcatat tggtacaagc ttaatcgggg
     6541 tcattattat ttactcttta ggaaatatgt tatggctatt tggtattcac caagcagtta
     6601 tttacagtgc catcctagaa ccattactat taattaacat tactgaaaac atcactgcag
     6661 caaataatgg acaagccatt ccacacatca tcaacctatc acaaatacaa acattcgctt
     6721 taatgggtgg tagtggatct acattatgtt tattaatagc aacattctta gtgagtcgca
     6781 atgctgtctc taaaaacgtg gctaaattat cttttggacc tggtatcttc aatatcaatg
     6841 aaccagtatt attcggttac ccaatcgttt ataacatttc attagctatt ccatttatca
     6901 cagttccagt ccttggtatt ttaatcagct acttagcaac agttacagga ttcatgagtc
     6961 ctgcatttat acaagttcct tggactacac cagtattctt aaatgcatgg ttagcaacag
     7021 caggggacgt gagagcagtt ctagttcaat tcatcatctt tgcacttgga gttcttctat
     7081 acattccatt tatcaaagtt aatgacaaag ttgttgaaca agaaatggaa ggttaagatt
     7141 atgaaacaaa ttttattagt atgtaatgcg ggaatgtcga caagtatgct tgttaaaaaa
     7201 atgcaacaaa gtgcgactga acgtgggatt gaaatttcta tccaagcaaa atcaatgaca
     7261 gaggcgaaaa agaatatcca tgaagcagat gtcattctaa ttgggccaca aattcgttat
     7321 gagttacttg ctgtgaaaga aattgcagga aatatccctg tagataccat tgatatgcgg
     7381 gattacggaa tgatgaatgg agcaaaagtt ctggaacaag cacttgcatg gataggagaa
     7441 ataagatgac agaagaaatg gaatatatct gttttcagct cattgccaat agtggagcag
     7501 ccaaatcatc ctttattgaa gccatccaac tagctaaggc aggaaattta aaagaagcga
     7561 aaattaaagt tgaagaagcg gaagactcct tagtagaagt gcacaagatt cactctaatc
     7621 tcatacaaaa agaagctact ggagaaaaaa taggattctc cttgctattt atgcatgcag
     7681 aagaccaaat ggcttcaacg gagattattc agttgctatc taaagaattc atcgatctct
     7741 atcaaaataa ataggaacac tgtcgttccc atctgtcttt attaatgacg gctacattgt
     7801 aggaacctcc aaaagttgga caaaatgatt gaaaggattg gatcctgcat tacacaagat
     7861 ccaatccttt tcttttactc tagtttttat aatatgttga atctagaata gtccactgta
     7921 gtttctaaaa aattgctgga aatggatgtt aagctccaat ttatttgttt atatcttatt
     7981 tcagtttact atactagctt attattctag tgaaaaattg aatgagccac tatttattta
     8041 atatgtatga tgagggaatg actcaaaaac ttcttgtcga tat
//
LOCUS       DAVKMU010000067         7741 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKMU010000067 DAVKMU010000000
VERSION     DAVKMU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.067"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..459
                     /locus_tag="VW343_001881"
     CDS             <1..459
                     /locus_tag="VW343_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9756392.1"
                     /translation="RYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLK
                     HHYQIYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHY
                     SNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(494..916)
                     /gene="nusB"
                     /locus_tag="VW343_001882"
     CDS             complement(494..916)
                     /gene="nusB"
                     /locus_tag="VW343_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9756393.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREYT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(909..1298)
                     /locus_tag="VW343_001883"
     CDS             complement(909..1298)
                     /locus_tag="VW343_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9756394.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(1320..1880)
                     /gene="efp"
                     /locus_tag="VW343_001884"
     CDS             complement(1320..1880)
                     /gene="efp"
                     /locus_tag="VW343_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9756395.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(2009..3451)
                     /gene="gatB"
                     /locus_tag="VW343_001885"
     CDS             complement(2009..3451)
                     /gene="gatB"
                     /locus_tag="VW343_001885"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9756396.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(3451..4917)
                     /gene="gatA"
                     /locus_tag="VW343_001886"
     CDS             complement(3451..4917)
                     /gene="gatA"
                     /locus_tag="VW343_001886"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9756397.1"
                     /translation="MTFNDKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDTDNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSASVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILSAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(4917..5219)
                     /gene="gatC"
                     /locus_tag="VW343_001887"
     CDS             complement(4917..5219)
                     /gene="gatC"
                     /locus_tag="VW343_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9756398.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            5432..6976
                     /locus_tag="VW343_001888"
     CDS             5432..6976
                     /locus_tag="VW343_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9756399.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            7231..7428
                     /locus_tag="VW343_001889"
     CDS             7231..7428
                     /locus_tag="VW343_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9756400.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            7539..7715
                     /locus_tag="VW343_001890"
     CDS             7539..7715
                     /locus_tag="VW343_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9756401.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
BASE COUNT         2175 a         1531 c         1762 g         2273 t
ORIGIN      
        1 cgttactgga aactcatcca acaggatagt cgtaaattga gcgataaacg tttttatcgc
       61 cctacttttc gcatgcactt aacaaataaa gaaattcttg acaagatttt aagctattca
      121 gaagacttga aacaccacta tcagatctat caactcttac tttttcactt tcagaataag
      181 gaaccggaga aatttttcgg actcattgag gacaatctga agcaggttca tcctattttt
      241 cagactgtct ttaaaacctt cctcaaagat aaagaaaaga ttgtcaacgc ccttcaacta
      301 cactattcta atgccaaact ggaagcgacc aataatctca tcaaacttat caagcgcaat
      361 gcctttggtt ttcggaactt tgaaaacttc aaaaaacgga tttttatcgc tttgaacatc
      421 aaaaaagaaa ggacgaaatt tgtcctttct cgagcttagc ttttcttcaa cccactacag
      481 ttgacaaaga gccttattgt tcttctgtta caaactggct gagcagtcca ttgataaaac
      541 gggcagattt ttgatcggag aagtcctttg caagctcgat agcttcatta acagcaacca
      601 gctgaggagt gtcaaatgaa gtgatttcaa agactcccaa gcgaaggagg tttctctcca
      661 cgagcgttaa gcgttcaatg gtccaacctg cttttaaatg ctgagtgatt tgcttatcta
      721 gttcttcctt tttagcttga acaccagaaa cgaggtctat caaaaaggct ggaagttgta
      781 catccgtata ttcacgatca tgagtatagg cgaaacgaca agcagtttcg acatccgtac
      841 cgaactcaag gctcatgaga gcttgaaaag cgcatttacg gagttggcgt ctagattcta
      901 atagtggact agtcattgag gaagtcctcg tcaaatagat ctttcaattc tggttttggt
      961 gttttatctg ggacgatacc tgcaacgtga atattgatag cagcgagttc tacatcagcc
     1021 atattacgga cggcatcttt gacagctttc tggatagcaa ccgctacctt aggaactttt
     1081 actccgtact caaggtagag atagatatct gctgtgagtt cttcgtccac gtttttaaga
     1141 taaatgccac ggccgagtga aagttttgaa agggtatcag acactgatct gtttgaaaaa
     1201 gagtgaacac cctctacctt tgcagtagcg atagcaatga ttttttcaag tacacgtggg
     1261 gcgataacga tttcgccaag ttgttcttca attcccatag aatgacctct ttctagagat
     1321 taggcacgag aaacgtaagt tccttctgca gtgttgataa cgagtttttg tcctgcttcg
     1381 atgaagtctg gaacgtttac gacaagtcca gtttccatcg ttgctggttt accagaacct
     1441 gtaacagtag cacctttgat agatggttga gtttcagcaa ctgtcaactc aacagtagta
     1501 ggaacggtga caccgatcac ttcagttccg tagaattgga ttttcacatc agagttttca
     1561 aggatgtaaa gcaattcgtt ttcaacattg actacaggga tttcgtattg gtcgtaagtt
     1621 tctgtattca tgaagtatgc tgtgtcatcc attttgtaca agtattgagc tgggacagtc
     1681 tcgataatag cttgttcaaa tttttcctct ggacggtagc ttgtgtcaaa tgtagaacca
     1741 gtacggacat cacgcaattt catacgcatg atcgtgtttc ctttacctgg tttgtggtga
     1801 ctagcttcca aaacgcgaat caatttgccg tcagctgttt caaaggtcat accagctttt
     1861 aatttacttg cttcaatcat gtttttacct ctttaataaa tattattact cttcatttta
     1921 ccataaaata ctctggaaat aaagccaaaa tagttattgg gagttggtag agaagaaaaa
     1981 accaacctta gggctggttt tgttctgtct agttttcttt caacttcgcc aattcctgtg
     2041 caagtagttt aagggcaact tgtgggttgg cttggccttt ggttgccttc ataaggaatc
     2101 ctgtaaaggc cttgtcggcg ttacgtttgc ctgacttgaa gtcggcaaca gcagcttcgt
     2161 tatcggcaaa gacttggtgg atgattggga tcaagatagc tggatctgaa atttgaacca
     2221 tacctgcttt ttccacgtat tcacgcgcgc caccgccatt tttagctaga tggacaaaga
     2281 ctttcttggc aatcttagat gaaatagtac cgtcttcgat gatggtaatc atttcaacca
     2341 agttttctgg tgtcaattcg atttgttcca gtgttttacc ttcagcattc aagaactgag
     2401 cgacttcccc ttggagccag ttagagactt gtttggcatc accacctagg gcaacagctt
     2461 tttcaaagaa gtcagaagtg actttattag cagtcaactg actagcatcg tagtctgata
     2521 aaccaaggtc agatacataa cgcgcacgac gttcttttgg aaactctggc aactcagtcc
     2581 gcatttcctc aatccactcg tcagaaattt caaagagggg taggtctggt tctgggaagt
     2641 agcggtagtc agcagcccct tccttgacac gcatgaggat ggttgttttg ttagcttcat
     2701 cgtaacggcg tgtttcttgg cggatttgac cacctgagcg aagaatttca gcctggcgtt
     2761 ggacttcgta ttcaagacct ttacgaacgt ttgagaagga gttgaggttc ttcaattcag
     2821 tcttggtacc gaatttttct tgaccataag gacgaaggga gatgttggca tccacacgca
     2881 tagaaccttc ttccatctta acgtcagaaa tgccagcgta ctggataact tccttgaggg
     2941 ctgtcagata agcataggct tcttcaggag aacgcatatc tgcctcagat acaatctcaa
     3001 tcaagggaac cccttggcgg ttgaggtcaa cataagagta gccatctgta ccatgggtgt
     3061 ttttaccagc gtcttcctct aagtgggcac gttcgatacc gattttcttg gtcgtaccgt
     3121 cttctagttt gacttcaatc cagccattat atccgattgg ttcatcaaat tgagaaattt
     3181 ggtaggcttt ggggttatca ggatagaagt agttcttgcg gtcaaagtgc atctttttgt
     3241 ggatgtccat gttgagggca agagcagcct tgataccggc atcaacaacc cctttattga
     3301 gaactggtag aactcctggg aaagaccagt caattacgtt agtgttggca ttttggtcat
     3361 ttccgaagtg ggcagaagta ggtgagaaga tttttgaatt ggtgttgagc tctacgtgga
     3421 cttcaagtcc gatgactgtt tcaaagttca ttagttgtca cctccaaaaa tcacgggttg
     3481 ttgtttgtgg tagtctgttg ttgcttcaaa agcagcagca gcttggtaaa tggtttcctc
     3541 agagtacttg ggaccaatca attggagtcc gacaggtaga ccttgagaga atccagcagg
     3601 aatcgaaatt ccaggcagtc ctgccaagtt tacaggtatg gtcaagaggt cggctaagta
     3661 catggcaact gggtcatggt tgagagaatc caagtcatag gcaacacttg gagcagttgg
     3721 acccaaaatc aaatcgtaat ccgcgaagac tttttcgaaa tcttgaatga taagtgtacg
     3781 gacctgtcca gccttcttgt agtaggcatc gtagtaacct gatgaaagac tgaaagtacc
     3841 cagcatgata cgacgtttta cctcttcacc aaaaccttgg cttcggctgt ttacatagat
     3901 ttcatcaagg ttggttgcat cttctgcgcg atagccgtaa cggataccgt caaagcgttg
     3961 caagtttgat gaagcttctg atgaagcgat gatgtagtat acggcaactc cgtatttaga
     4021 gtgaggaagg ctgacttctt cgacaatagc accaagtttt tcaaagtgtt tagcggcact
     4081 tagaatggtt tccttaacct ctgggttaat tccttcaccg aggtattcct taggcaaagc
     4141 gattttcata cccttgatgt cttggccgat ttttgaagta aagtcggcga tgcggacaga
     4201 agcagaagta gagtctttag catcttcgct ggcaatagcg ttgagcaaga gggcattttc
     4261 cttaacagta ggagcaaaag gtccaatctg gtctaatgag ctgccaaagg caatgagacc
     4321 gaaacgtgaa actgttccgt aggttggttt gagaccaacg attccgttga aggcagcagg
     4381 ttggcggatg gaaccaccag tatcagaacc aagtgacaag cgaacttgtc ctgaggctac
     4441 agctgcggca gaaccacttg atgacccacc aggaaccttg ctgtggtccc aagcgttttt
     4501 agttgctccg tagtgtgaag tttcacctga accacccata gcaaattcgt ccatgttggt
     4561 ctttccaacg acaatcatgc ccttggtttt tgcattggca acagctgtcg catcaaagat
     4621 tggctcatag ttgtagagca tttttgaggc agcagttgtg agaataccgt ctgtagagat
     4681 gttatcctta acagcaagtg gaattcctga aaggacattg tcagtatcaa ttccagcttc
     4741 atcaatggct ttagcttgaa caagagcttg ctcctcagcg atggtgacaa atgaattgag
     4801 ggcttcctca cgagacttga tattttcaag tgttgcttgg gtcaattctg ttgcagaaat
     4861 ttccttagag acaaggagat tgtgcaactc ttcaatagtt ttatcgttaa aagtcattag
     4921 gcatctcctc catcgtctag gatagctggc accttgatat agtagttgtc tttttcaggt
     4981 acgtttttaa acaagcgatc acggtctgtt ccttcttcgg ccacatcagg gcggagtaca
     5041 gtcttgcggt cagccatagt cgtagtaggt gcgacaccag ttgtgtcaac ttcgcccagc
     5101 aattcaacca tgtcaacaat cttagacaag gtggtcgcaa aggcagcagt ttcttcttca
     5161 gagaatctta attttgaaag attggcaacg tgtgttacct cttcttgcgt aattttcatc
     5221 ttgcatcctt tcgtgaaatg atgattttta gtctgttcta ttttaccata ttttcctata
     5281 aataagggag ggggaagtga aaagaaatag aaattgaaaa aaatgctaaa atccgatata
     5341 atggagtgtt gaaaggaatg gtaaaatcca atgtaaaaat cattctcagc tattgaaaca
     5401 agaaaaatag agaatcaaag aaagagaact tatgaatatt caagaagaaa ttaagaaacg
     5461 tcgtaccttt gccattatct cccacccgga cgcggggaaa acaaccatca ctgagcagtt
     5521 actctacttt gggggtgaga ttcgtgaggc tggtacggta aaagggaaga aaacagggac
     5581 ttttgctaaa tctgactgga tggatatcga gaagcaacgt gggatttctg ttacttcatc
     5641 tgttatgcaa tttgactacg acggcaagcg cgtgaatatc ttagacacgc cagggcacga
     5701 ggacttctca gaagatacct atcgtacctt gatggcggtg gatgctgcgg tcatggtcgt
     5761 ggactctgcc aaggggatcg aggctcaaac caagaaattg tttgaggttg tgaaacatcg
     5821 tggcattcca gtctttacct ttatgaacaa gctggaccgt gacggtcgtg agcctttgga
     5881 tctcttgcaa gaattggaag aaatcttggg cattgctagc taccctatga actggcctat
     5941 cgggatgggg aaagcctttg agggcttgta tgacctctat aaccaacgtt tagagcttta
     6001 taaaggggat gagcgttttg ccagtctaga agacggggac aaactctttg gtagcaatcc
     6061 tttctacgag caagtcaagg atgacattga gcttttaaat gaagctggga atgagttttc
     6121 agaggaagct attctggctg gagaattgac gcctgtcttt ttcggttcag ccctgacaaa
     6181 ctttggtgtg cagaccttcc ttgaaatctt cctcaagttt gctccagaac cacatggtca
     6241 caagaaaaca gacggtgaaa ttgtggatcc ttatgacaag gatttctcag gctttgtctt
     6301 taaaatccaa gccaacatgg atcctcgtca ccgtgaccgt attgcctttg tccgtatcgt
     6361 atcaggcgaa tttgagcgcg gcatgagtgt caatctccct cgtactggta agggggctaa
     6421 actatctaat gttacccagt ttatggcgga gagtcgtgag aatgtgacca atgccgtggc
     6481 aggtgatatt atcggggttt acgataccgg tacttatcag gttggggata ccttgacggt
     6541 tggaaaaaac aagtttgaat ttgaaccact gccaaccttt actcctgaaa ttttcatgaa
     6601 agtttctgct aagaatgtta tgaagcaaaa atccttccac aaggggattg agcaattggt
     6661 gcaagaagga gccgttcagc tttataagaa ttaccaaaca ggtgagtaca tgctgggagc
     6721 tgttggtcaa ctccagtttg aagtctttaa acaccgtatg gaaggcgaat acaatgcgga
     6781 agtggtcatg agcccaatgg gtaaaaagac cgttcgttgg atcaagcctg aggacttgga
     6841 tgaacggatg tcgtcaagtc gcaatatctt agccaaagac cgttttgacc aaccagtctt
     6901 tctctttgaa aatgactttg ccctccgctg gtttgcggac aagtatccag acgtagagtt
     6961 agaggagaag atgtgactca gtaccaacaa ttggaactaa agttccaaat tgttggacgc
     7021 tagtcgctat ttggcgaact agctaactgc ctcactaact acgtttggca aatgaaatcg
     7081 atttgtaaaa ctccgtgtcg taatgtaaaa attgtaaacc gtaatgggac ttcatctaaa
     7141 atctccactg gtatggtttt acgaacgaat aggcgaaaat aatggttacg tagaaacata
     7201 tgatgggaat aaaaagaaat gagataaaaa gtgaaagaag agaaaaaagc gattgtctta
     7261 ggtgcagata atgcttatat ggataaagtt gaaacaacaa taaaatctct ttgtgttcat
     7321 cattataatc ttaagtttta tgtttttaat gatgaccttc cgcgagaatg gttccaattg
     7381 atggaaaaaa gactagagac tttgaatagt gagattgtga atgtttagat tgacagtaat
     7441 attctgaaag gttatcgtct cccctttgag ggactttcat atgcggcttt tttaggtatt
     7501 ttattccaaa gtatgtaagc gagtctcgtg tactgtattt ggattcagat attgttgtaa
     7561 gaaaatcgat agatgagctg tgggatttgg acttgacggc tataccctta gctgcagtta
     7621 gagatgattt ttatacacat aattttaatt ctggtgttct gttaatcaac gatggcatgt
     7681 ggagagcaga aaatgtcacg caagacctga tttaagacac aaataaaaaa tcttccaatt
     7741 c
//
LOCUS       DAVKMU010000068         7712 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKMU010000068 DAVKMU010000000
VERSION     DAVKMU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.068"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(177..1592)
                     /locus_tag="VW343_001891"
     CDS             complement(177..1592)
                     /locus_tag="VW343_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9756402.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAKLLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1605..3443)
                     /locus_tag="VW343_001892"
     CDS             complement(1605..3443)
                     /locus_tag="VW343_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9756403.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVIPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     DVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDESVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3461..4303)
                     /locus_tag="VW343_001893"
     CDS             complement(3461..4303)
                     /locus_tag="VW343_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9756404.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLT
                     KNLD"
     gene            complement(4603..7281)
                     /locus_tag="VW343_001894"
     CDS             complement(4603..7281)
                     /locus_tag="VW343_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9756405.1"
                     /translation="MAVFVSLDGIVVEVLDVFSSFDGDSEFFLCKRLKDKSQFVMERS
                     RFEEMFQLQSSHLTTQEKLQLFTSVFAGRYDVYAKNFINEQGKIQYFPSYDYGWKQLP
                     PEKRSFQTLTNSVLKSHFRGETAIGIFPMHLDDSCHFLVLDFDEGDWKEAGLTIRRIA
                     RERQMEAHLEISRSGHGLHIWFFFEEAIPSREARLFGKKLIELAMQESMQLSFDSFDR
                     MFPNQDVLPKGGFGNLIALPFQGEAYHQGRTVFVDEQFQPYEDQWRYLQEIQRVSTAK
                     VALLIQEELGKQELDKGLKIVLSNMIQLEKSSVTPKTLFFLKNMASFSNPEFYLKQAM
                     RQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVVVEDRRKVQRSISVEFKGEL
                     TFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKTKTIILVHNKQLLDQWLDR
                     LNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKLVDVVMIQSLFKLENSQSL
                     LDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPERKNGHEPIVFQRIGEILH
                     TADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWIATDSARNQLILKDILAQV
                     AEGRNILVLVNRIQQIDVFEKLLKEKEVDDCYIISGKTKVRERTSLLETLEQLDKGFV
                     LLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIHRNYKDKSLVRIFDYVDIH
                     VPYLEKMFQKRQVAYRKMDYRAIEGEEKQFVYVDSRYEKVLIEDLAGERQECLLILPY
                     VHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKIKVSFTQSKIVTPILLVNK
                     TIVWYDAMPLLGKVDEMTILRLESASIVSELVAGLR"
     gene            7530..>7712
                     /locus_tag="VW343_001895"
     CDS             7530..>7712
                     /locus_tag="VW343_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9756406.1"
                     /translation="MKLTYDDKVQIYELRKQGYILEKLSNKFGINNSNLRYMIKLIDR
                     YGIEFVKKGKNRYYSPD"
BASE COUNT         2543 a         1598 c         1070 g         2501 t
ORIGIN      
        1 ataaccaatc tatcctaagt taactattta taatcggctc tctgtaacaa aattaaatta
       61 attaatcccc ctataaaata aagaggttta gaatttgatg tcttccaaac ctctttattt
      121 catattcaat gagatgttgc cttaaccgtg ataatatcta atcatcaata aacaaactat
      181 ttgaataagg attctccatt tgattcaatc actcctttat accaagtaaa ggacattttc
      241 ttatatcgat tgaatgtgcc atttccatca tcatttcgat caacataaat gagaccgtac
      301 ctcttagaaa gctgtgcagt ggacatagaa acgcagtcaa tacatcccca agacgtatag
      361 cccataattt ccacaccatc ttgtagagct tcagctactt gcaataaatg ttctttcata
      421 tactgaattc tataatcatc ttggactgtt aagttattaa attcatcttt tattagttga
      481 tctttagcac ctaatccgtt ttccactata aataatggaa tttgataacg gtcataatat
      541 ctatttaaaa ttatacgtag tccaattgga tcaatttgcc atccccactc agaagactct
      601 aaataaggat ttactaagcc accaataata ttcccttctc ctgaattata ctgtgttgga
      661 agagcagatt gagtcacact catgtaatag ctaaaggata aaaaatctac ggtataattt
      721 tttaataact ttgcatcttc agctgcaaac tctatgttaa tgtcattttc cttaaaatat
      781 ctttttgcat aattcggata ataacctcta acatgcacat ctgaaaatag ataatttaga
      841 ttctcatact catgagtcgc ccatacatct tttggatttg gagtcattgg ataagctggc
      901 atagctaata ccatacaccc caccttaaac tctgaattaa tctcacgagc aatttttgta
      961 accaaactcg aggcgactaa ttcatgatgt atagcttgat ataattcttg tttcgaaaga
     1021 ttctccttag gtatatctat tcctccacta gtaaacggta attccaaaac agagtttact
     1081 tcgttaaatg taagccaata tttaacttta tctttatacc tttctaaaac tgttcgagca
     1141 aatttttcat aaaaatgaat cattctccta tcaatccatc catgatattt tcttgctaaa
     1201 tataatggag tctcatagtg tgaaagagtt acaagtggtt ctatcccgtg agcatgtagt
     1261 tcatcaaaca attcatcata atatttcaac ccagcttcgt taggttcttc ctcatctcct
     1321 tttggaaaaa ttctactcca tgcaatagaa gtacgaaaaa cattaaagcc catttcagaa
     1381 aacaaggata tatcttcctt atatttatga taaaaatcaa tacctatcaa ttttaagtta
     1441 tcttctgtag gattttctgt tgcttctcct aatccacctt tgggtaacac atcctgaact
     1501 gataagccct taccatcttc attatatgct ccctctactt gattagctgc aacagctcca
     1561 ccccaaagaa aatcatctgg aaaaatggtc ataactttcc tccattataa tattaccagt
     1621 aattccttag aatgctcgat tgtctgatta ttaggtaata ctaatacatc tagaaaatca
     1681 ttggtattcg ttacaattac tggtgtaact gtttcgtagc ctttagtctt gattaaattc
     1741 aagtccattt caaaaatcaa ctgatttttg aaaactctgt ctccttcttc tacatgacta
     1801 ataaaacctt gaccttttag ctcaacagta tctaatccaa tatgaattag taactcaaca
     1861 ctctcatcac tcttcaatcc aattgcgtgc ttagtcggaa aaatatttgt aattttccca
     1921 tcaaatggtg cataaacctt accttcactt ggaataatcg ctactccgtc tccaattagt
     1981 ttatctgaaa atgttttatc ctggacatcg cttaacggaa tgatttctcc tgatatagga
     2041 gaaaatatca tttttttatt tgaaactcca gctttaactt ctaaattgct agaactctct
     2101 tcttcatcga ttccaaatat ataagctaat acaaaggtaa taacaaccga aataaccgcc
     2161 acaattaaag catttacaat atttgatggt acatcagaat aaataaattg aggcaacgct
     2221 atcaaagatg ggacagcaaa tagatacgct ttaacactag taagacctgc aaataatccc
     2281 gctaatccac caccaatcat agctgcataa agcggttttt tatattttaa agtcacacca
     2341 tataatgcag gttcggtaat ccctgcaagt aaggctgaga aacctgctgc aaaagcaatt
     2401 tgttttgtat tattattttt actctttaat gcaacagcca tcgaagcagc cccttgagct
     2461 aagtttgacc ctaacattgc tggaagaatt aatacgtctg gagtagcaat agatgccgcc
     2521 aaaaaaatag gtgcaaaagc ccaatgcatt ccagtcataa caataaatgg cataatagca
     2581 ccaagaatag ctaatgtaag ccatccagct acatcataca tttgcccaac tagatttgat
     2641 aatccttcac caacaattac tccaataggc ccgactacaa ctaaggcaat acagcttgat
     2701 actaataata ctagcgtagg ttgcaaaaaa ctcttagtaa cagctggtgt taatttagca
     2761 atcatttttt caatatattt catcaaccaa accataataa gaattggaac gactgacgaa
     2821 ccataactag ctggtatcac aggtacacca aataaactaa gaggattccc tgattgcacc
     2881 atttgaacaa aatttggatg gagaagtaca cctgctacag acatagctaa tgtagatgtt
     2941 acttttaatt tttgtgatgc agaataagct aataacagcg gtaagaaata atatggagca
     3001 tccccaaaaa atgtcaaaaa agcaatagtc tgagaatctg attgcaatat accaagcatt
     3061 ggtaaaataa ttaccaagac tttcagcata cctcccccta acattgctgg aatgattgga
     3121 gtcatggaac cagcgatata ctcaatgatt ctttctaaaa tattcccttt gtgcccttga
     3181 acaactgaat cggattcaaa attgccaagt ttaacgaatt ctttataata attagctaca
     3241 tcattaccaa gtataatttg atattgtcca ttctttttca taatacctat tacacctggt
     3301 atcttcttca catcatcatc attgactaaa ttttcatctt ttaattctaa tcttaaacgt
     3361 gttacacaat gggtaactct attgacattt ttttcacctc caattacatc gaggattttt
     3421 tgtaccttat ctttataact catggtattc tcctattcta ttaatctaaa ttttttgtta
     3481 agcgacgaat atgagccatc aaataaacta attcactaga agtcagcaaa taattgtact
     3541 cagtttgtat aaacattgcc acctgttcac cacattcata ttctctagga tatttatttt
     3601 tcattaatgc taacaagtct tcatcatcat catcgtcaca ataagttgta ttttcaacca
     3661 agcgatgggc aaataatttt atatgagtta tgaatctata ataatcaata gaatcttggt
     3721 taaactcagt acaatagaaa tcttgaatga ttttctcaat tcccataatt atttcagtga
     3781 ttttatacga ctcttgaaaa ttattttcta aattagcatt aacaaaatgt agcgctatga
     3841 atgcagcttc gtcaattgga agttcaatac ctaactcttt ttttattatt tgtaaagccc
     3901 ttttcccaag ctcaaattct tctttataat atttcgagat ttcccagcgt aggggattcg
     3961 aaataataac tccttcttta taacgttcta tactcgaaga aatatgatcc gttagattta
     4021 tatagataat ctcatcaaaa ttattaccta atgttatttt acctagatta attatttttt
     4081 cggtgatgta caccaactcc aaaggaacgt taataaacat ctctgtaaaa cgggtcatat
     4141 tatctctatt tttgagaaaa aaacttttct caatatcaat tggatttact ttatctccaa
     4201 tttttcttcc aaaagctatt ccccttccaa aaagaagaat atcgatatct tttttacttt
     4261 gcgcaattac ggcattgtgg tttagtattc gttttatgat cataaaattt cccttttaaa
     4321 ataaaaggaa cctacttaaa aacatcaaga aacttcttct gatgtgatta aataggttcc
     4381 tgctcacttg tattagtgat aacatccttt tatttcaatt tttaatggtg acatgaagct
     4441 taaataatta ataactctgt aaccattttc attttaagat atttcaatct atttgtcaag
     4501 tacttgtaga aattttttta ttttgtgtga ttcgaatgtg ataatggcaa cagtctcact
     4561 aaagaaatga aaatcataca tagaaatttt taaaaatttt ctctatcgta aacctgccac
     4621 tagttcagaa actatactag ctgattccaa acgtaatatg gtcatctcat ctaccttccc
     4681 taataatggc attgcatcat accaaacaat agtcttattc accaaaagaa taggcgttac
     4741 tatttttgat tgagtaaaag acactttaat tttctggctc ttcaactggt ctagccatgc
     4801 tttatttgca accgtctctg gtatacatat ctcaatttga ctaatcctaa attcttttag
     4861 aaaattcatc agttttgtct ggtgcacata aggtaaaata agcagacatt cctgtctttc
     4921 ccctgctaag tcctctatca acaccttctc atatctacta tcaacataaa cgaattgttt
     4981 ctcctcaccc tcaatggcac gataatccat ctttcgataa gctacttgtc gtttctgaaa
     5041 catcttttct aaataaggaa catgaatatc cacataatcg aaaatacgca ccaaagactt
     5101 atccttgtag tttctatgaa tccgacctgc atactgaatt aaattatttt tccaagaaaa
     5161 gggtgctgcc aagataagcg tgtccaactg aggtaagtca aaaccttcgc caatgtattt
     5221 tccagtagac aacaaaacaa accctttatc taactgttct aacgtctcca gtaaactcgt
     5281 tctttctcgg actttggttt ttccgctaat aatgtaacag tcatcaacct ctttctcttt
     5341 caataacttt tcaaagacat ctatctgttg aattcgatta actaaaacca agatattccg
     5401 tccttctgcc acttgggcta gaatatcctt gagaatcaac tgattcctcg ctgagtctgt
     5461 agcaatccaa tcactgagct gtataaaatt acttgctttg gtcttttcaa tctccaaatg
     5521 accaaaagaa gtgaatctta attgcaattg ccgtttaaaa tccgtttccc tcttatcagc
     5581 agtatggagt atctcaccaa ttctctgaaa aacaataggc tcatgaccat tcttacgctc
     5641 aggcgtagcc gtcaaaccgt aaagatactt ccctctaaac tgagcaacaa ctttttcaaa
     5701 catcaaggca gagacatgat gacactcatc cacaatcatc atctcatact catccaaaag
     5761 actttgacta ttttccaact taaatagaga ttgaatcata acgacatcaa ccagtttact
     5821 cagccatttc ttagtcccac cgtactgtcc aacatagccg attacctttt cacgacctga
     5881 tgccgtataa cggatagcct cctcctcttc gaaagtcaaa aagcagttta agcgatctag
     5941 ccattggtct aagagttgct tattatggac tagaataatt gtttttgttt tccgttcaga
     6001 gataagagca gcacctaaaa cggtcttccc aaaaccagtc tccgcatgaa gtaaaccatt
     6061 ttctttagaa gtcatatctg acagggctaa ctcttgctca aaagtgagtt ctcccttaaa
     6121 ttccacgcta atagaccttt gtacctttct cctatcttcc acaactacct gcttaaattt
     6181 atcttgcaat ggatacagca aacctcttgg caaccataaa taatgatcgg attctccaaa
     6241 taaatacatt ctctcaggaa tttgataggt tggctgtcgc atagcctgct ttaaataaaa
     6301 ttcgggatta gaaaatgaag ccatattttt caagaaaaac agtgtcttgg gtgtcacaga
     6361 cgatttttca agttggatca tattggataa gacgatcttc aaccccttgt ccaattcttg
     6421 cttgcctaac tcttcttgga ttaacagtgc cactttagca gttgaaaccc tctgaatttc
     6481 ttgtagatac ctccattggt cttcataagg ctgaaactgt tcatccacaa agaccgttcg
     6541 cccttgatgg taagcttctc cttgaaaagg caaggcaatc aaatttccaa atcccccctt
     6601 aggaaggaca tcctgatttg gaaacatgcg atcaaaagaa tcaaaggaca gttgcatact
     6661 ttcctgcatt gccagttcta tcagtttctt tccaaacaag cgagcctctc gactcggaat
     6721 cgcttcctca aagaagaacc aaatatggag tccgtgaccc gaacgagaaa tctctaaatg
     6781 ggcttccatc tggcgttccc tggctattct tcgaatggtt aaaccagctt ctttccaatc
     6841 tccttcatca aaatccagta ccaaaaaatg acagctatca tctaagtgca taggaaagat
     6901 accgatagct gtctccccac gaaaatgaga tttcaaaacg gagttcgtca atgtctggaa
     6961 actccgtttt tcaggtggca actgcttcca accataatca taggaaggaa aatactgaat
     7021 tttcccttgt tcattgataa aattcttagc ataaacatca taacgtccag caaacacgga
     7081 ggtaaacaat tgtaattttt cttgcgtcgt caagtgacta ctttgaagtt ggaacatctc
     7141 ctcgaaccga gagcgttcca taacaaactg actcttgtct ttaagacgtt tacacaagaa
     7201 aaactcacta tccccatcaa aagaagaaaa gacatcaagc acttctacca caattccatc
     7261 caaagacaca aaaacagcca taagagtcac ctccttgatt cctataggct gattataaca
     7321 agaatagctg aaattgtaca cgaaaataaa atccgaatag cactcatttt gtatgcgact
     7381 aatagtccat ctcgctccgg aatgtacgag gtaaatagag tttaaacgag cagtctataa
     7441 aattcaaaaa acgcatatta ccaaggttgt tttgaactac accccaaaag ttagacagaa
     7501 aaaatctaac tttttggggt gtttttatta tgaaattaac ttatgatgat aaagttcaga
     7561 tctatgaact tagaaaacaa ggatatatct tagagaagct ttcaaataaa tttgggataa
     7621 ataattctaa tcttaggtac atgattaaat tgattgatcg ttacggaata gagttcgtca
     7681 aaaaaggaaa aaatcgttac tattctcctg at
//
LOCUS       DAVKMU010000069         7707 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKMU010000069 DAVKMU010000000
VERSION     DAVKMU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.069"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(782..2002)
                     /gene="divIB"
                     /locus_tag="VW343_001896"
     CDS             complement(782..2002)
                     /gene="divIB"
                     /locus_tag="VW343_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9756407.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQKDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGVYSYTVADKLIMEAEEKAKKEAEEAEKRQEEERKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            complement(2012..3070)
                     /locus_tag="VW343_001897"
     CDS             complement(2012..3070)
                     /locus_tag="VW343_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9756408.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(3074..4426)
                     /locus_tag="VW343_001898"
     CDS             complement(3074..4426)
                     /locus_tag="VW343_001898"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9756409.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(4530..5171)
                     /locus_tag="VW343_001899"
     CDS             complement(4530..5171)
                     /locus_tag="VW343_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756410.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(5173..7194)
                     /locus_tag="VW343_001900"
     CDS             complement(5173..7194)
                     /locus_tag="VW343_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9756411.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLIDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(7265..7564)
                     /locus_tag="VW343_001901"
                     /pseudo
     CDS             complement(7265..7564)
                     /locus_tag="VW343_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         2308 a         1686 c         1319 g         2394 t
ORIGIN      
        1 ctaagaacaa tataaaatct taaataatat ttcttcccta ctaacactaa taagtccgat
       61 atggtaaatt cttgacctat tagttgctct aaccattttt ttcctgtttc ccaactaata
      121 catgtttctt actggatggt atatatagat gatactgtcc ctcataagga gtaggtaata
      181 tcttaatctt atcaacaatt ctcataacaa acctaacctt taataaataa aagaggggct
      241 aagtaaaaca tactctagtc cctcagtccg caatcaataa aataatgact aattctatta
      301 atctcttgct gcaagactaa tacggaaaat tcaaatgact gtctaaattg aaaaagcttg
      361 atattagtaa gtgaacaaac ttcaacaacc agcaagagca aggattgcta aaccaaatcc
      421 gatacctgtt gcaagtccag ctgaaaaatc actaactggt actgaaattt cttcaatttc
      481 ctcaaataaa aagttatctt gcatgattat cactccctat ctttttaaca acaggctact
      541 ccaattaaga gaactacccc agttccaagt aaaactcccc cccaaaattc accatctgta
      601 ggggctacga tttcttcaat ttcttcaaat aagaatactt catctgacat atgtgttacc
      661 ttcttcataa aaaatttttt tgtaatcgat tacattgcaa atgtaacata aagaaaaaca
      721 gatgtcaaat attaaacgta aaaacatggc aactaaagag ctataggaga aaaggtaaaa
      781 cctagcgacg cgatgagcgc tgggtcgttt ggtttcgatt gctctcttcc tcttgttttt
      841 tctgttcttc ttctaaacgt ttacgttcct cttcctgcct tttctcagct tcttcggcct
      901 cttttttagc tttttcttct gcttccataa tcaatttatc cgctacagtg tagctgtaga
      961 ctccagcttc catgtcaatc acactcggtt ctgacaattg tggcttaatc ttactgtaat
     1021 atggcagttt cttactcatt tcagatagag gaaccaagac ttcgtccgaa tcattcatgg
     1081 tcaatcgaat taaatcggat gtcaccttgc ttggggctaa ttccaccttt tggatagccg
     1141 ccttgagttc tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt tgttcactat
     1201 cattaaagag aactgataaa taagtttctg gtaaactgtt cagactcaca gaactagtct
     1261 caagctgacc actggaaaga ataggataat gattttcacc agaaatatag taggccacaa
     1321 tatcatattc cttgacctta atagtgaact tagttggaaa ttgatagaca agttgagctg
     1381 attcaaccca atagttagac ttaatctgct tttcatattt tgccttgtct agcagaaggt
     1441 taatcgtata atccgaatcc tgaatgcctg aagcctgtcg aatatcatca gctgtagttt
     1501 gcaccgttcc ctcaacacga atatctttca tggtcgcata aggactgagc aagtaggcag
     1561 agacaatcaa taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag atatggatac
     1621 caggaatctt tgctttggct ggtttttcct ttgtagcctt tttagcaagc tttttatcct
     1681 gttcctcctt ctctttagac tctggttctt ctttctcttc tttctctttg tcagcctctg
     1741 aggatgctac tttttcttca gactcttcct tagctgattc tgaatctttc tggtctgttt
     1801 cactctcctg gtcctgttta tcctctgact tctcagattc ttctcccatt cgagcttgtc
     1861 tttccttttc cttctcctca gctagagccg cctcttcttc agccttcttt tttagatatt
     1921 cttggtttcg tttctgccat tctgataact ctttcaattc ttcgagggtt tctttgtcct
     1981 catttttctt atcttttgac atttactttc cttatgataa atcttttttc aacaattgat
     2041 aaaaatctgc tagagatttc aattccttag aagctttcat cttagcttgg taatcttcct
     2101 tgtgacttag taagtgagaa agcttctctt ccaaactatc caaggtcaaa tcgctttctt
     2161 gaaggtcttc tgcatagcct ttcttaacaa agtaagctgc attttcaatc tggtcaccac
     2221 gactagcttc acgaccaagc ggcacaatga catgcaattt tgctatcgcc aagagctcaa
     2281 aaatcgtatt ggcaccacca cgtgtcacaa caatatcagc caattccatc aagggttgat
     2341 agagatcggt cacatagtca acacgaaaaa gattttggct caactcattc aggctagaat
     2401 ctccagttag attgataata ttgtagcgct ctgttagttc ttttttatgg tctgtcacca
     2461 attggttaaa gacacgagca cctgcagaac cgccaacaaa caatacagtc ggcaatttat
     2521 gattaaagtg gctttgaata tccaccaatt catctggttc tggatttttt tgatctgaaa
     2581 ccttggtcac tgctcccaca tgctcaacct tagccaaact cgaagcttgt tcaaaggttg
     2641 aatacatctt agtcgcaaat ttataggcga ttttattggc caagcccata gacaggtcag
     2701 attcgtgaat aaagacaggc actcctgaca cacgcgcagc gataacaggc ggtactgaga
     2761 caaagccccc ctttgaaaaa agggtctgtg gacgaagtcg caacatgata aagagcgatt
     2821 ggacgattcc ccagccaact ttgaagacgt ccagcatatt ttgccaagag aaatagcgac
     2881 gcaatttccc agtcgcaatg gagtggaaag tgacatccaa acctgactta aggatttctt
     2941 ggtgttcgat accacgcttg tccccgatat agtggacttc ccaaccatct tcgatgaact
     3001 tgggcattaa caaaagattg agggtaacgt gtccaaccgt ccccccacct gtaaagacaa
     3061 tttttttcat attttattct tttaactccg ctactgtgtc gataaagagg tcgccacgta
     3121 cttcaaagtt agcatacata tcccagctgg cattggcagg actaagaaga accacatctc
     3181 cttgagtcgc aagctcatag gccttgcggg tcgcatctgc aatatctgtc gcctccacat
     3241 aagcgacacc agccttgtct gctgcccgtt tgacacgttc tgcagattga cccaggatga
     3301 ccatcttctt gagtccagta atatctggca ccaattcgtc aaactcattg ccacggtcca
     3361 aaccacctgc aatcaagacg accttgctgt tgtcaaatcc tgacaaggct ttttgagtag
     3421 ccaagatatt agttgattta ctgtcgttat agaatttaac acccttgatg tcatccacaa
     3481 actggagacg gtgtttgaca ccaccgaagg ctgaaagagt ttccttgatg gtttgattgt
     3541 ccacaccacg aagcttggct acagcaatag tcgcaagggc attttccaca ttgtggctac
     3601 ctggaacacc gatttcattc gctgccatga ctacttcacc acggaagtag agttgaccat
     3661 cttccagata agctccatca accttttcaa gtgttgaaaa tggtacaaca gtggcttctg
     3721 tcttggaagt caagtctttt gccaagtctt gattaaagtt caagacaagg aaatcagctg
     3781 ctgtcatctt gttctggata ttccacttgg ctgctacata ttcctcaaat aatccatgat
     3841 aatcgatatg agttggcata aggttagtaa taaccgcaat ctctggatga aattcttgaa
     3901 cacccatgag ttggaaagaa gaaagttcca taacaagcgt gtccttgtcc gacgcagttt
     3961 gggccacttg actagctgga tagccgatat tccctgataa aagaccatgt tgcccagcag
     4021 cagtcaaaac ttccccaatc atagtcgttg tggttgtctt accgttcgat cctgtgatac
     4081 caataatcgg tgcttctgaa atcaaataag ccaattccac ctcagtcaag actggaattc
     4141 ccttggccaa agccttttca atcatgggat tgttgtaggg gatacctgga tttttcacca
     4201 taagggcaaa ctcttcatcc aagagttcca aaggatggcc acctgtaatg accttgatcc
     4261 cttcttccag caaactttgg gcagctggat tgtcctcgaa tggtttcccg tcatttactg
     4321 tcactatggc gcctagctta tccaacaaac gagctgcaga ttcaccagac ttggccaaac
     4381 ctaaaacaag aactttctta tttttaaatt gatctattac tttcatgtct cgaactccat
     4441 ttctactcct actattttac catttttatg gaaataaaaa agccacaaag tgtgtttgtg
     4501 actctttctt ctaactgaat cttaccatat tatctatgtg ataaatcggt aactcgaatg
     4561 acctgatcca cttgctccca aatcagagga ttgtgggtcg caataataat ggtccgattc
     4621 ggatttttta aagattctag gatggaaagt aattcctcag agtttttggg gtctaaggaa
     4681 gcggttggtt catctgcaag aatcaaaggc ggatccttta aaattatctt cgctaatgca
     4741 acacgttgtg cttctcctcc tgataactca aatataggtt gcttcaaatc caaataagag
     4801 aggtttacac ggtttagagc ttgtttcatc aaagagattt tctctttttc cttcaacttt
     4861 ttaccaacta aacccagatt gagattctct ttgacggttt ggctttcaat taagccaaaa
     4921 tcttgaaata agtatcccaa gtaatctcta aagaaaacag aaggcttgat gtccttaaga
     4981 gaagtgccat catagatgat ttgccctttg tcatatggct ctaatcgtcc aatcatattc
     5041 aagagtgttg tcttaccaca gccacttgta ccgattaagg cataaatttt cccaccttca
     5101 aaatgaagat ttgtatctga aaatagctga cggcttccaa attttttaga tatattcttt
     5161 agttcaatca tcctattttc ctttcataat tgtcatagaa acacgagatt ctttctgcgc
     5221 ttgacggtaa agcgtcagaa ctgcactagc tagaaagact aataaagtga gcaagccaat
     5281 caccaagtct cgactgctta aaataaagag actagcacca aatacaaaac tggcaaattg
     5341 actaaccata tactgagcat gtgtttcaaa aaatcgtaaa cctgaaattc gtttaatcaa
     5401 aatatctcgg cggaattgct cgaaatatag aagattgaca gaataaaata gtaacaagga
     5461 actagctatc cccacaatag ctcctaagat taaagttgct gtttcagttt gaacttcatt
     5521 ataacgagtt agataaacac ttcttccttc tttaagatag gatacttgct cataaattcc
     5581 agctttcttc aagagttcta gcccactctc atatcctttg ataaagagtt gttttccagc
     5641 attgatagac caactagata aggatataaa actatcacct gtagaagtcg gcgtgaatac
     5701 aactaaaatc ggatcaatca aatactgagt ggatacggga ttctcaccgt tattataaac
     5761 aaaccgcttt tctcccattg aaagataact aacgtgcgct ttcatctcat aatccaaagg
     5821 agcacttgcc tcctcaccag attttccata ataactcaat ctttcttcaa aaactttctt
     5881 aagttctgct tctcgagagc gcaaatgttc tgggagcaag aggataaact cacctttttg
     5941 gagatgggct aacttctgtt tggtctcagc atctaccacg accttttcct tgtccaaata
     6001 actgggacta acatagagcg tattagcatc tggactatag gtatccagtg tctctccctg
     6061 ttcatttttt ccttgtggat tggcaaaatg gagcagatta tcctttacat aaagagcttg
     6121 ttcttcttcg attgcttcct tggcaaaggc ataccacttg ctctgatttt ctgtatcttt
     6181 tcctctatca cctaagccaa aagaaatttg ataatagtct gctctatcct gccatgcttg
     6241 ttttgaaatt tcaagttctt tcaatcgttg gtaagacgtc aaacctgtct taacagcgta
     6301 tcctactgta aagacagcaa ccaactgaca caatagggtt aaaatcatca ggcgtttaag
     6361 gggtaatctc cccttaataa cgggaactaa tgctttgtaa ttcaaactca ttagataaag
     6421 aagcattagt aaaattgaaa tcgccaataa aaacaacaga tagaaactaa tcccaaaacc
     6481 ataggtggct aacaagatag gataaaacaa accttgacta aaaagaacga ctcccccacc
     6541 taggaaggaa aggagggctg atagaaggag ccatttgata tcagtagata aagaatgccc
     6601 catgatggat aagagagtct gaccagaaaa gagttttata cctgctgctc tcatttcctt
     6661 aatccgagtg ataatcacta aagcaaagaa agataaacca aatattgcta aactaattaa
     6721 aataagggga tttagtaata ttcgaaaagc aagaaaatag ggcggtatct ttcggtcagc
     6781 acttgcttta taacccaaat ctcctaattt atcggcaagc ttttctttcg tcaaggagcc
     6841 tgacaaaagg agataactat ttagcggatc actacgttca cgactttctt ggctagcttc
     6901 ttggaattct tttggtaaag ttccctgacc ataagttgca taagtaaagt gagtcgtccc
     6961 atccttactc ggctctacaa ttcttctagc tattaaactc tgttctgagt ttgcaaaatt
     7021 ctccaattcc tgttcaaata cctcacgcgt cggttcctga gtatcttttt tgacacgaag
     7081 taaagaaacg gaatcatagc ttgcatataa atattgtggc gcacgtaaga caataatcca
     7141 agcaaggaag aagctgagaa aaaaagttga taataatatg aatagtttct tcatagtaga
     7201 ctccttgtaa acaaaattcc ccctgtaatt tcttacaagg ggaacgattt aaatcaatga
     7261 acgattagtc ataatcacag taaaatgcta cttgttctcc ccatttagtc caaatccatg
     7321 cataggaggt ttttccagca ggagcttctc ctttatcaga atgaccatcc catctgctca
     7381 cgatggatga ataatgatat tttttaccat gatagtaatt tgaaaaagcc taaccacctc
     7441 ctgaaccttc tccatatgtc catactcctc catctggata ttgtacagca gctgatgcag
     7501 ctcccaataa tgtaaaattt gaaataagag ctagagcaag taatctatgt tttttcgttt
     7561 tcattttatt ttttctttca aaaaaatcac accttgagca acaatgcaac aaaataaatc
     7621 ctcctctctc ttttgttgaa atcgctttct tatgtgataa gaataacatt tttattattt
     7681 gttgtcaagg aaaaaatcga atttttt
//
LOCUS       DAVKMU010000070         7645 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKMU010000070 DAVKMU010000000
VERSION     DAVKMU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.070"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..846)
                     /locus_tag="VW343_001902"
     CDS             complement(1..846)
                     /locus_tag="VW343_001902"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9756412.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKACAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            1102..2129
                     /locus_tag="VW343_001903"
                     /pseudo
     CDS             1102..2129
                     /locus_tag="VW343_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2430..4319)
                     /locus_tag="VW343_001904"
     CDS             complement(2430..4319)
                     /locus_tag="VW343_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9756413.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGTEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKEQSKQLLTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFERDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLLGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            4457..6022
                     /locus_tag="VW343_001905"
     CDS             4457..6022
                     /locus_tag="VW343_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9756414.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYANAKSLDDFNGAKITSQQGVYLYD
                     LIAQITGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMVQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTITGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            6022..6762
                     /locus_tag="VW343_001906"
     CDS             6022..6762
                     /locus_tag="VW343_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9756415.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(6871..7086)
                     /locus_tag="VW343_001907"
     CDS             complement(6871..7086)
                     /locus_tag="VW343_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756416.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         2417 a         1703 c         1355 g         2170 t
ORIGIN      
        1 ttatacaaat aaacggacaa aactgtaaag tagcaaaaca ctaccaagca cgatacggta
       61 tttaccaaaa agggtgaagt cgtgtttttt cacatagctg gtcaagaagc gaatagccac
      121 catgctgacc gcaaaagcta ctcccatcgc gaccaagagc aaaaacaatt gcccaaagct
      181 caagagttct ccggctttca caaatttgaa aatctttaag gcactagctc caaacataac
      241 gggaatccca agatagaagg taaattctgt cacaactgaa cgactggttc catttaacaa
      301 accaccgaca atcgttgcac ctgaacggct agtccctggt aaaagagcaa gaacttggaa
      361 gagtccgata tagaaagcgg tcgtataagg aagcttgtcc aactctgtta cacttggctc
      421 gatagcacac gctttattgc gcttttccaa atagatgaag gcaaccccgt agataatcaa
      481 catgagagca actgaaacca tgttatggaa gtgggtatca aaccaatcat caaatttaaa
      541 gacaccaagt aaaggtaaag tagcaatcaa gaccttcaac catagtctcc aagtcttacg
      601 aacttcctgt ttgtccttag tcggtttaaa aggattgagc ttgttaaaat aaatcaccat
      661 aactgctaaa atagcaccaa gctgaatcac gacattaaac atggacataa aggcttcatt
      721 ttgattttgg tattggataa actcctctgc taaaatcaag tgacctgtac tggaaatcgg
      781 caaccattcc gtaattcctt caacaatccc gaagaagata gattttaaaa tttcaataag
      841 atacatatat ttctccttat attatattta acgcagttag ctactgccaa agcccaagtg
      901 gtatacttgg aataagccac tgtggattag ttcatttcct ccaatcgctt gacgatttcc
      961 gaaaggatct aaccacagct ttttgtttca attggtaatg agtttgataa cgcatagacg
     1021 ttttatctcg tctaaggtaa caaggcaaaa agttcgtgga taaccaagta gacttgaaat
     1081 actatatctg gaggtactcc catgctttat gtgggcattg atgtcgctaa aaataaacac
     1141 gatgttacag ccttgaatgt tcgaggaaaa ctgttcttaa accactcact ttttcaaata
     1201 ataaagctgg ttttgaacta ttagatctgt ctcttcgaca gctcaaccaa gactgtctca
     1261 tcgctcttga agataccgga cattatgcct ttaatctact gaacttcctg catgaacaag
     1321 gatataaagt ttacacctac actcctttgc tcatcaagga atttgccaaa tcattatcac
     1381 ttcgcaagac taaaacggat aagaaagacg ctcatggcat cgctcttaaa cttctttctg
     1441 accccaatcg tgaacaattt caacacgata atcggcaagt agaactaaaa atactggcta
     1501 gacatattca tcgtctcaag aaaaaacagt ctgattggaa agtacaatac actcgttgtc
     1561 ttgatatcat ctttcctgag ttggataaaa tcgttggaaa gcattcagaa tatacctacc
     1621 aactcttgac atgctaccct aatcctcaga aaaggcttga ggcaggattt gataagctga
     1681 tagaaattaa gcgattgacc gcttctaaaa ttcaggatat cctctcagtc gcacctcgtt
     1741 ctatcggaac aacatctcct gctcgtgaat tcgaaatcat cgaaaacatc aaacattaca
     1801 agaggctcat tgacaaggcg gaaaaatgtg tcaatgactt gatggctgag ttcaactcgg
     1861 tcatcacgac ggttactggg attgggaatc gtttaggggc ggtcatttta gccgagattc
     1921 aaaatattca tgcctttgat aatcctgctc aattacaagc tttcgctgga ctggattctt
     1981 ctatttatca gtcaggtcag attgatttag ctggaagaat ggtcaaacgg ggttctcctc
     2041 atctgcggtg ggcactcata caagctgcca aagcatgcgc tcgcttttca cctgctttta
     2101 aggcctatct taagactaag ttagaataag aaaaacatta caatgtagcc atcatccacc
     2161 ttgcaaaaaa acttatccga accctgtttt atatcctaaa aaagagctgc catttgacga
     2221 acaaaaagtg atgtaatttc tgaaatcaac acaggattac ctttgaaaaa atggactttt
     2281 tcaagggctt tttgccgtgc tctaaaatca tcgccaaatc atcaaattga aataagaaat
     2341 agtttagata aaattattga cttttcttat agtttgtctt tcttctatct tccattatat
     2401 catacttttt cagtctatgg tagctctctt taaaaggctc cgatactgcc gccacctccg
     2461 cctccagaga agccaccgcc agaacttcca cttccagaag atacggagta ggtacttgct
     2521 gtatttgcga cactagcata atggctcatt tgcgcgcttg aatgataaaa catactgtgc
     2581 cagccataag ctacatagag attgatatct ggattttcaa cttgaatctg atgaaccttc
     2641 atcaaatgac taaccttgtc cgcatagcca agtaaggttg catagaccaa gagacgattc
     2701 caaacaacaa tactttccaa ttcagcctta tccagtcgtg caatctcacg taacatattt
     2761 tcaaaactgg tccagagata gtagacctca gctcccgctt catttagaac accatcacga
     2821 ttatctagtc gaagcttcca ataatagaaa acagacaaaa ctaaccctag aaaaccaagt
     2881 attggcaaag ggaggtaaag atagccatga acgtctaaac tgtacaagaa caaaccaaat
     2941 ccgataaata ggggcaggat agtcaaggca cccataccca cttgcaaggc cttttcccca
     3001 ccagttaaag gacgataata atctgggagc ccccagaagg aaactcgttt tctcactcct
     3061 tcttgcatct ggttcaatac ctcttcaaaa gaagatttga gttgaagccc tcttgcttga
     3121 atccgttttt catcagaaac tttggctcta cgataaagac tatcagatac cttgtaatcc
     3181 gcaaacaaat tggaaagagt ttcttctttt ttacctgaaa aggctagatt tagacagtct
     3241 ctctcaaagc ttgacaaacc atcttctttt actagcctca aaccaactgc atctccttct
     3301 gaaatgatag agacattccc acggtctatc acatctagca aggtagcttg aataagttga
     3361 tcaaaggtga attttccagc tcccttgacc aagggactca cttcctccaa ggaggtcgag
     3421 tagactgctt ctgataaaac cataggctct aattccattg gtggttcata gagacgatga
     3481 tttttggcat atttgactga aggagtggtc tttcttctat aaataaaata gaagcagaca
     3541 ctcaataaca aggagatgga aaggatcgaa gggaggaccc aagtaagtag ttgtttactt
     3601 tgctcttttt ctctaacaat cgagtcttct atcttattaa actcttctaa tcgattccct
     3661 ttcaatccct gatccctagc gctagcaaaa tcggtccgag gccaataggc atgcaactca
     3721 actccacgct tagccggaag attgtctaaa cggatagtat aatcaaggtt actcttttca
     3781 atcgttccct ctctaaaaag tttccctgta tggaaaaaga gtttttcagt ccccttgtct
     3841 ccccttacat gaaattcaaa cttttcaata gactctgaac tatctgtcag aggttgccaa
     3901 tttaattcag cgatatcatc ataaaggaaa agtaaatttt ttaagttcca gacgaggtca
     3961 acttcaacta tgtcgccctc ctgacctgga ttatagactc tcacagtata accatccgct
     4021 tcttctgtta cttcgctagt cacatctgct agttctgcac cgtttttcgc ggcctgaatc
     4081 tttggatgag ggtcaatgtc aaacccgcta ggcatcttac cagcacgtcc aagtcccacg
     4141 atttggccct taaagtcctc ctcaaactgg taaactatct tctgtctaaa ctctgccgta
     4201 ttatctgcat gaatatacaa atccccttga taagagttta tcttgaaatc aatggcaaaa
     4261 acagagagtg gaagaaggca aaacaagcct aacaccagta agaaaaaagt ttttttcatc
     4321 aactaagccc cctttttatc tttcttatca ttatagcatt ttttcgtaag taaaggctta
     4381 cttatgttga aaaattacgc atttttcgat aaaataggag acagttattt tttaatagat
     4441 cagaatagga gaaatcatga gaaaaatata cttatctatt ttcacaagtc tcttgctgat
     4501 gctaggactt gtcaatgttg ctcaagccga tgaatattta cgcatcggga tggaagcagc
     4561 atatgctccc tttaactgga cccaggatga tgatagcaac ggagctgtca aaatcgatgg
     4621 gaccaatcag tatgccaacg gatacgatgt tcaaatcgcc aagaaaatcg ctaaggactt
     4681 aggtaaagaa cctttggttg ttaaaaccaa gtgggaaggt ctagtccctg cccttacttc
     4741 tggtaagatt gacatgatta tcgcaggtat gagtccaact gcagaacgca aacaagaaat
     4801 tgccttttcg agcagttact acactagcga accagttcta ctggtcaaaa aagattctgc
     4861 ctatgcaaat gctaaatctt tggatgactt taatggtgcg aaaatcactt ctcaacaagg
     4921 tgtctacctt tatgacttga ttgcacaaat cacaggtgct aaaaaagaaa cagccatggg
     4981 agacttcgct caaatgcgcc aagctcttga ggctggtgtc atcgacgcct atgtttctga
     5041 acgcccagaa gctctgactg ccgaagccgc taactctaag ttcaagatgg tccaagtaga
     5101 accaggtttc aaaactgggg aagaagatac agctatcgcc attggacttc gtaaagatga
     5161 cactcgtatt agccaaatca atgccagcat tgaaaccatt tcaaaggacg accaagttgc
     5221 cctgatggat cgtatgatca aggaacaacc tgccgaagct acaacaactg aagagactag
     5281 cagtagtttc tttagccaag ttgctaaaat tctttctgaa aactggcaac aactcttgcg
     5341 tggtgctggt atcactcttt taatctctat cgtcggaacc atcacaggtc tcattattgg
     5401 acttgccatt ggtgttttcc gtactgctcc tctctctgaa aacaaagcca tttacggcct
     5461 acaaaaacta gtcggctgga ttctcaacgt ctacattgaa attttccgtg gtacgccaat
     5521 gattgttcaa tcgatggtta tctactatgg aactgcccaa gctttcggga tcaaccttga
     5581 ccgtacactg gctgctatct tcatcgtttc aatcaatacc ggtgcctaca tgactgaaat
     5641 cgtccgtggt ggtatcctag cagttgacaa gggacaattt gaagctgcga ctgctcttgg
     5701 tatgacccat aaccagacca tgcgtaagat tgtcctacct caggtagtcc gtaacatcct
     5761 acctgcaact ggtaatgaat ttgtcatcaa tatcaaagat acatctgtat tgaacgttat
     5821 ctctgttgtc gaactttatt tctcaggaaa taccgtggca acacaaacct atcaatactt
     5881 ccagacattt acaatcatcg ccgtgattta ctttgtcctc accttcaccg taacacgtat
     5941 cctacgattt atcgagcgcc gaatggacat ggatacctat actacaggtg ctaaccaaat
     6001 gcaaacggag gatttgaaat aatgacacaa gcaatccttg aaattaaaca cctcaaaaaa
     6061 tcctatggac aaaacgaagt gctaaaagac atttcactca ctgtccacaa gggagaggtc
     6121 atctctatca tcggaagctc tggaagcgga aaatcgacct tcctacgctc cattaaccta
     6181 cttgaaacac caactgatgg acaaatcctt tatcatggac aaaacgtcct cgaaaaaggc
     6241 tatgacctca cgcaataccg tgaaaagttg gggatggttt tccaatcctt taacctcttt
     6301 gaaaatctca atgttcttga aaacacaatc gtcgctcaga caactgtcct aaaacgcgaa
     6361 cgcacagaag ctgaaaagat tgccaaagaa aacctggaaa aggtcggcat gggagaacgc
     6421 tactggcaag ccaaaccaaa acaactctca ggtggtcaaa aacaacgtgt ggccatcgct
     6481 cgtgccctct ccatgaatcc ggacgctatt ctctttgatg aaccaacatc agctctcgat
     6541 ccagaaatgg ttggagaagt cctcaaaatc atgcaggacc tggctcagga aggcttgacc
     6601 atgattgtcg taacccatga aatggaattt gcccgtgatg tctctcaccg tgttatcttt
     6661 atggataagg gcgtgatcgc tgaagaaggt aaaccagaag acctcttcac caatcctaaa
     6721 gaagaccgaa caaaagagtt ccttcaacgc tatctcaaat aaaaagaaaa ggctgcatca
     6781 accgtgcagt ctttttgctg tcctcaaaat gaaaaggcag attctctatc tctactactt
     6841 gaaggtcaag aatccgccgt tatccaaaga ttaatcttca aatttttcat aatttttttc
     6901 atagaaatca attaggccta gggctgtttc aaatgaaata ttttttactt ttgcacgacc
     6961 ctgcgccaga gcaatgatag acatttcacg cgcattcgtt tctttagaga tacggtagcc
     7021 tgtgattttc ttatcacgaa cccagccaac aactgattct actttttcaa agtttgactt
     7081 agccatgttt ttctcctatt ttcttcttta taatatttaa ttgtatacgt ttttactact
     7141 tttgtcaata aaaaaacatg tattcaacga aaataattag ttcagaattt tagcttcaga
     7201 attttcttac ttcctttttg aatccgataa tatataggtt ttactagtct ctacaccata
     7261 aatatttgct aataaaaata ctgtattatt accctcttaa ggtgcgaaat tattcaagtt
     7321 cttaaaaaat gcttctctat ttccgtaaga tagcttattt cttcgattta agaactgaag
     7381 atgatagttc taacctttag aaatccaagc cttcactagc tcaaaaactc ctatctttct
     7441 tcctcaataa atcatctgag agataggtat tccttatgtc cttcaaattc atcactcctt
     7501 tggatattga ggtgctagcc tagaagcaga cacccgatta tctaaaccat ccttaataaa
     7561 gatgacaaga cagataagct cagttaatta cttatatatc tttttaatat ggtatatttg
     7621 tcaacaaaaa gaaatcaaat catac
//
LOCUS       DAVKMU010000071         7086 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKMU010000071 DAVKMU010000000
VERSION     DAVKMU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.071"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..211
                     /locus_tag="VW343_001908"
     CDS             <1..211
                     /locus_tag="VW343_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9756417.1"
                     /translation="TPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPP
                     LALQNEFADFVVQVDKSQFACEIAI"
     gene            279..3629
                     /locus_tag="VW343_001909"
     CDS             279..3629
                     /locus_tag="VW343_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9756418.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFGENCCVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIEEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3900..3977
                     /locus_tag="VW343_001910"
                     /pseudo
     CDS             3900..3977
                     /locus_tag="VW343_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4215..4685)
                     /locus_tag="VW343_001911"
     CDS             complement(4215..4685)
                     /locus_tag="VW343_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9756419.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4702..6975)
                     /locus_tag="VW343_001912"
     CDS             complement(4702..6975)
                     /locus_tag="VW343_001912"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9756420.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHINKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2178 a         1203 c         1521 g         2184 t
ORIGIN      
        1 gacaccaaat ctaaatcaga aatttattat ccatgtttta aggaataata attatagtcg
       61 agtgatatca ggaagtgctc agcctcagtt accaattaca aaattaaaaa aaatacttct
      121 ccccctcccc ccactagccc tccaaaatga gttcgcagac tttgtagtcc aggtcgacaa
      181 atcacaattt gcttgtgaga tagctatata aaagtgtgga gaaatagctt gaaatttagt
      241 ataatatagc taaactattt gtttaaagtg agaaaaaaat gggaaatttt agctttcttt
      301 taaaaaatga cgaatatgaa tctttttcaa aaccttgcat tgaagctgag aatatgattg
      361 ctacatcaac tgtggctact gcctttatgg cgcgtcgtgc tttagagcag gctgtccatt
      421 ggatatatag tcacgattca tatttagaag ctccctatcg tgctactcta tcttctttag
      481 tatgggatga tgattttagg gatatcgtag attctgaact ccacaagcag atagttctgt
      541 tgattcggtg gggaaaccat gctgctcatg gtggtgaaat taaggaacga gaagcgattt
      601 tagctttgca tcatttgtat cagtttgtta attttatcga ttattgttac agcaatgagt
      661 ttgtggagcg ttattttgat gagaagtgct taccactttc agcaaacatc aaataccgag
      721 aaactccaca atctatgata aagttacaag acagtttacc agaactgcct gattttcatg
      781 aacagatggc tgctcagtcc gtagaagttc aagagactta tactgaaaaa cgtgagactg
      841 cagcgcaacg gcaagatgtg cctttccata ttgatcaatt atctgaggca gagacaagaa
      901 agctctttat tgatatcgat ctccgtttag caggatggat atttggagaa aactgttgtg
      961 ttgagatagc cgttgatggt ctcaagcacg gttcaggaat tggttactgt gactatgtac
     1021 tttatggtaa aaatgggaaa attttagcga ttgtggaggc taaaaaagcc tctgtcaatc
     1081 cagaagtagg ggaagtacag gtcaaagaat atgctgaagc tttggagaaa catatcggct
     1141 atcagccaat ttgctttatt acaaatgggt tgaagcacta tatacttgat ggtccgaacc
     1201 gacgccagat tgcaggcttt tactctcaag aagaattgca attagtgatg gatagacgtc
     1261 atcttcaaaa accacttgag gatatttcta gtaaaattag ggacgatatt tccgggcgtc
     1321 actaccaaaa acatgccatt gcaagcgttt gtgaagcttt ctctaatcat cgtagacagg
     1381 cacttttggt tatggcaact ggggcgggga aaactcgtac agcagtttct ctagttgata
     1441 tcttatcacg tcataactgg gtaaaaaacg ttctcttctt agccgataga acttccttgg
     1501 ttaagcaagc ctatgattcg tttagaaaat tactcccaga tctttccgtt tgtaacttct
     1561 tagaagataa agaaggagct caatcaagtc gtatggtctt ttcaacttat ccgaccatga
     1621 ttggagcgat tagtggtcaa gaagaagtaa atcaacgccc ttttactgtt gggcattttg
     1681 accttatcat aattgacgaa tctcaccgtt ctatttatca gaaatacaag tccatttttg
     1741 attattttga tgcaagaatt gtaggcttaa cagctactcc gcgtcaagat ttagataaaa
     1801 acacctatgg attctttaat ttggagaatg gggttccaac atatgcatat gatttggaag
     1861 aggctgttaa agacggatat ttagtagcct atcattctat cgaaaccaaa ctgaaactac
     1921 ctacggatgg tctacattat gatgatttgt ccgaagaaga aaaggaacat tttgatagca
     1981 aatttgaaga cgatagctgt gaaaaagata ttgatgggag tgtatttaat tcctttgttt
     2041 tcaataaaag tacagtagaa attgttttaa atgaactcat gacaagagga attcagacag
     2101 cctcgggtga tgaaattggt aaaactatta tttttgctaa aaatcatgat catgcagaat
     2161 atatcagagg tatttttaac aaccgctatc ctgaaaaagg gagcgactat gctcaggtga
     2221 ttgattatag tattaagcat tatcagacct tgattgatga ttttaaaatt gaggagaagt
     2281 atcctcaaat tgcgatttct gtcgatatgt tagatacagg tattgatgta ccagaggttg
     2341 ttaatttagt cttcttcaag aaagtacgct ctaaaactaa gttttggcag atgattggtc
     2401 gaggaacccg tctatgtaaa gatttatttg gacctgagca ggataaggaa aacttcttgg
     2461 tatttgatta tggggataat tttgattatt ttcgtgcaga tccaagagat ggagagggtc
     2521 gtcacattgt ttcgttgact cagcgtttat ttaatatcaa agtggacttg attcgagaac
     2581 ttcagggact ccaataccaa gaagatcagt ttgcgagagc ataccgtcag cagcttgtct
     2641 cggaacttca aggtcgtata gagagcttaa atgagttgga cttcagggtt cgtatggttt
     2701 tagatacagt ttatagctat aggaaattgg aaagttggca gaatctaact gctgttacaa
     2761 gtgaaaccat tcaaaaaaat ctctctccgc ttttatttga tgaagataaa gaagatgaga
     2821 tggccaggag atttgatttg tggttgcttc atattcagtt ggggcaactg acagctaaat
     2881 cttccactgt tcatatttcc caagtgatga agacggctag agctctttct gctattggca
     2941 atatcccgca ggtttttgag caggctgaaa ttatcaggaa agtacaggag cctgaatttt
     3001 ggaaagaagt taacttgtct gatttggaaa aaattcgtct tgctattcga gatttattac
     3061 agtttttgga taaaacagac cgtaaaccct actatgttaa ctttgaagat cgtatactct
     3121 ccactgttca cgagaccaca gcatttttgc aggtcaacga tcttcggtct tacaatgaaa
     3181 aagttgagca ttatttgaaa actcatctgg atgaggagtc catttctaag ctataccata
     3241 ataaaaagtt gacatctgat gatatgcttg cacttgaaaa attgctttgg gaaaaattag
     3301 gtagtaaagc agactaccaa agtcattatg aaaataaggc aattccgaga ttggttcgtg
     3361 agattattgg cttagataga gagtctgcca atcgtatttt ttctaaattt ttgtcggatg
     3421 agaatcttaa tgccaggcag atttcatttg taaaattgat tgtagactac attgtagaaa
     3481 atggtttttt agagacgaaa gtgttaacgc aagagccgtt taaatcttat ggttctgttc
     3541 aactactctt ccaacaccaa ctaccagtac ttcgtaatat tgttcaaatc attgaactta
     3601 tcaataatcg agctggagaa gcggcttaaa ttctaaagtg attgccatgc tgagactcat
     3661 ttaaaattaa aaagagtaga aatttatgct atatatgaga agttttatta ggaagaatgt
     3721 catcgttttc ctagaataca gtatcagttg ttaagtggtt gataaatttc aaagtagata
     3781 cttgtaccac gatgtttgtt gatcgagtta ttaacaaaag agctactttg attttaaaga
     3841 aatagaaaac aaaaagccga gcaagaattc aattgcagga gaaaatgaaa taatactcaa
     3901 tgaaaatcaa agagcaaact aggaaactag ctgcaggctg ctcaaaacac tgttttgagg
     3961 ttgcagataa gactgacgaa gtcagctcaa aacatggttt tgaggttgta gatgaaactg
     4021 acgaagtcag taaccatacc tacggcaagg cgacgttgac gcggattgaa gagattttcg
     4081 aagagtataa atcttcctag gataaagcaa aacgcatagt atcaagggtt ttcaacactt
     4141 gatactatgc gttttctgat gttaaagact ttctaccagt ttttttaagc ataattgtta
     4201 gttgtagtca tttattattc ttcaaagaaa aatggtgggg cgaatttttt cagttcttca
     4261 aagcactttt gagcagtatc tgcatcttca cagatgataa gacagacatc attaccacaa
     4321 agggtagcga tagcgtcagg gaagctcaaa gtatcaatga tagaaccaaa ggattgagcc
     4381 agtccaggaa gggtttttag taggacttgg tgttgaactg ggcgcatcca gacaagggcg
     4441 tcttccatgt agagttcgag acgtttttcc cattttgaga tggaaccatt gttaagaaca
     4501 taataagcgc tatcttcttc gcggactttt gataggttca tatttttgat gtcgcgtgag
     4561 agggttgcct gggttacttg aatgtcgttc ttagcaagaa gggcttgcaa ctcagcctgt
     4621 gtatgaatct tgttttttgt gataagagcg cgtataagtt ggtggcggtg ttctgattta
     4681 ttcataataa tgtaactcct tttagcaagg taaggtaagc atggactgag cgaggtcgac
     4741 agtcaagtgg tagtctgtat tgtcacggat ggtgatttca aagtcagtag tatagaggac
     4801 taaacggaga gtgtctcctt cttttagctt gtaaatagtt ggctgcagtt caaattgaac
     4861 atccatccat tcatctgcag taatatcctc tactaacagt aaatcatttc tattttgtaa
     4921 attaaggtaa ccttttgtca cgactcgttg tgcctctggt ctaaatggca attcacagag
     4981 attttccaac atgtgatagc gaccgttgtc aatggttcta gcacttaaaa tagctggata
     5041 aggttgtagg tatttctttt gcccaaattc tagcagttgg gcagataaga gccccttgtt
     5101 tgtactggat ttgatacgaa gattgagctg agcgcgaccg tttaggtgga gatctttagt
     5161 cacaggaagg ttaatagtaa tctgattggc tttcccttga tagagctctg tattgaaggt
     5221 ttggtatgtc ttaccatagc gctcaaaatc cttatctggg tactggtttt gaatagcttg
     5281 ctcttcttga ccaagtgaga aggtttcaca gttttcttgc ccaccgaagt tatcaagtga
     5341 taaccaagtc tgtggagctg tattgtcctg ccagataaca gtaggaagtt gaaagtctgt
     5401 ttcctgtcct agtaatttct tggtcaataa ggcatttatg gactcacgga agtcaattga
     5461 ttgccaattg ttcatgtaaa catgggcacc attatggaaa aagagatgct tgtttatatg
     5521 agtaggaaga gcatggaaca tctggtaaac atgaagtggt ttgacattcc aatcctgaga
     5581 accatgagta aagacaacct ctgcctttac tttatgggca ttgagcagat aattgcggtc
     5641 atgccaaaac tgattgtagt ccccagtttt tcggtctagc tgagctttca ctttttctaa
     5701 gtcagcttgg tgagcttcat tgccacggat atagtcgcca gctaagagat tacgagaata
     5761 ggttaactca gcaagggagt caaagtcctc acctggataa ccacctgggc tagtcaccag
     5821 accgttttca cggtagtagt tgtaccatga tgaaattcct gcctcggcaa tgataacttc
     5881 taaaccatcg actcctgtag tcgcaagacc attggacatg gtacctagat aggaaagtcc
     5941 tgttgtagca acttttccgt ttgaccaatc agccttgact tgacgctggc gcgtgtgatc
     6001 agtaaaggca cggcaacggc cgttaagcca atcgatgaca tttttataag cctcgatttg
     6061 ctggtagtct ccattagtca tgaaacctgt cgagtctttg gtaccaacac ctgagacata
     6121 gagattggca aagcctctcg gaaggaagta gtcgtttagt gtatagctag agttgatgtg
     6181 agttagcttt tcctcagcct ctgctataag ctcagcttta ccttggggtt ggacgagatt
     6241 tagttgaggt ttctctagct caatcttgtg aggaagctta acctcaagct cgccctccat
     6301 cttgtagaga gccttgtcac tagccttgtc attggttccc tgatgataag ggctggctgt
     6361 catgatggca gggatttttc catcaaaacg agggcgaata atgctaacct ttactaggtc
     6421 tgatagccct ttttggtcag tatcgacacg agactcaacg taaacgactt cacgaatgac
     6481 atcctggtta gaaaaagtag ccaaactctt gccgttaaag tagtggtagt cattatcctc
     6541 cggaataaga ccatcactaa caagttggtc gataagagta tttccttttt tggtgcgagt
     6601 attgagtaac tgatagagat tttcaatcaa gtcaccatat ataatgggaa atccagtttc
     6661 tttacgaaaa acgtcactat cttcgaagtc aaccaaataa gaaaagccta aaagttgaaa
     6721 agcaacagta taaaaaatat ctgctgtcag ttcatcttct gattgaaaaa atgtcagcag
     6781 gtctgttttt ttatcagctg ctaggataga aagtgggtag ttggtgtctt gataagtgaa
     6841 aaagaaacga cgtaaaaagg tttcaagtga gtctttgtga ttggctgtat tttgtaaatc
     6901 aaagccacat ttttttagtt cagataagac attttctttt ggaaaattga tataactata
     6961 ttgattaaaa cgcatagaac ctccatatag aatgacagtt aaggttatta tatcaaaaaa
     7021 aaagcagaaa gggaattgtt aacttcaaaa ggaaataatc caataaaaat gaataaagta
     7081 ctaaat
//
LOCUS       DAVKMU010000072         6917 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKMU010000072 DAVKMU010000000
VERSION     DAVKMU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.072"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(106..381)
                     /locus_tag="VW343_001913"
     CDS             complement(106..381)
                     /locus_tag="VW343_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9756421.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(488..1327)
                     /locus_tag="VW343_001914"
     CDS             complement(488..1327)
                     /locus_tag="VW343_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9756422.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1469..2317
                     /locus_tag="VW343_001915"
     CDS             1469..2317
                     /locus_tag="VW343_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9756423.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2410..3327)
                     /locus_tag="VW343_001916"
     CDS             complement(2410..3327)
                     /locus_tag="VW343_001916"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9756424.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3673..3881
                     /locus_tag="VW343_001917"
                     /pseudo
     CDS             3673..3881
                     /locus_tag="VW343_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4191..4484)
                     /gene="rpmA"
                     /locus_tag="VW343_001918"
     CDS             complement(4191..4484)
                     /gene="rpmA"
                     /locus_tag="VW343_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9756425.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4501..4845)
                     /locus_tag="VW343_001919"
     CDS             complement(4501..4845)
                     /locus_tag="VW343_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9756426.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4861..5175)
                     /gene="rplU"
                     /locus_tag="VW343_001920"
     CDS             complement(4861..5175)
                     /gene="rplU"
                     /locus_tag="VW343_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9756427.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5211..5298)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5363..6061)
                     /locus_tag="VW343_001921"
     CDS             complement(5363..6061)
                     /locus_tag="VW343_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9756428.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
     gene            6463..6762
                     /locus_tag="VW343_001922"
     CDS             6463..6762
                     /locus_tag="VW343_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9756429.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2174 a         1394 c         1154 g         2195 t
ORIGIN      
        1 ttcggaacat tcaacttttt aactctattt attactagat ttcataatta aaaagcctac
       61 tgaccaagct agaaagcttg atacaatagg ctttttaaag actgattatt taacagcgtc
      121 tttaagagct ttaccagctt tgaatgctgg tactttagaa gctgcaattg tcatttcttt
      181 accagtttgt gggttgcgac ctttacgttc tgcgcgctca cgaacttcaa agttaccaaa
      241 accgatcaac tgaacttttt caccagctgc aagatagtca gctactgctg caaatacagc
      301 ttcaactgct gctgctgagt ctttcttagt taattctgta gcttctgcta ctttagcgat
      361 caaatcttgt ttgtttgcca tgttaatgat tcctccaaat aatttctaat taacaaatat
      421 aatcatatcc taaaatccct tttaggtcaa gttaaaaacg ctatttcttc ccattttatt
      481 tattttttta ggagtggtaa cgtatcaaaa tagcccaagc gttctcaccc gtgtgagttt
      541 gaataatgga acccgtttcc aaaacagaaa ttggcttttc aacataagct tgtaagcttt
      601 ctttcatctc ttttgcccaa tcatcactac cagaatatga aattccaatc tctgctacag
      661 cacgttcaga aagcgatgtt atcaactcat ctaaccattt tttaaatgtt ttagttccac
      721 gacctttaac cattggctgc aattcatggt ctttcatttg catgacaaca cggatattga
      781 gaagagagct caacaagcca gttacacggc taattcgtcc acctttgaca agattttcca
      841 aagttgaaac accaatataa agctctgtat ggtttttaac ctcttctaca tgagataaaa
      901 ttgcctccat atctttacct tcttgagcta acttcgcagc ctcaacaact tggaatttca
      961 gggcttggtc agtgaaggaa ctatcaacaa cagtcacatc tgcagtagat aggctagcac
     1021 cttggcgtgc tgcttctacc gtacccgaaa gagcatggga catatgaata gcaagaatct
     1081 ggccaccatc tttgcatagg tcttcaaaaa tctcagcaaa gacacctaca ggtggctgac
     1141 ttgttttcgg aagattctta ctttcttgca tcaactgaag aaatttacct tcttctttca
     1201 aatccgcatc agaataaaca acattatcaa tcattacaga taatggaaca attgtaatat
     1261 ctaattgctt tactagttca ggttcaatag taacagatga atcggttaca atcttaattt
     1321 ttgtcatagt atcaatcttt ctattttagg attcagattg gtttccttac ttctaattat
     1381 atcaaaaaaa agattaaaaa tcctaatgga gtcaatcaaa ttttccgtaa aatttgatat
     1441 aatcaactta taagaaaaga ggtgtcctat gattaaaaaa atttacccca tttttaccat
     1501 tttactaggt gctgctattt atgcttttgg actgacttat tttgtagttc cccatcatct
     1561 ctttgaagga ggggcgacag gcattaccct catcaccttt tatcttttta aaatccctgt
     1621 ttccctcatg aacctgctga ttaatattcc ccttttcatc ctagcttgga agatctttgg
     1681 agccaaatcc ctctattcta gtttactagg aaccttagct ttgtccgcct ggttagcttt
     1741 ttttgagcat attccccttc atattgatct tcaaggtgat ttactaatca cagcccttat
     1801 agcgggaatc ctattgggaa ttggccttgg aattattttt aatgctggag gtacaactgg
     1861 cggaactgat attctagctc gtattctcaa caaatacact catatatcca taggaaaact
     1921 gctctttatc ttagattttt gtattctcat gttgattctc ctaatcttca aggatttgag
     1981 attggtttcc tacacgcttt tgtttgattt tattgtttct cgtgttattg atttgattgg
     2041 tgaaggagga tatgccggca aaggctttat gattatcaca aaacgtcctg accaacttgc
     2101 taaggcgatt aatgatgacc tcggaagagg tgttactttt atttctggtc aaggctacta
     2161 tagtaaagaa aatttgaaaa tcatctactg tattgtcgga agaaatgaaa ttgtgaaaac
     2221 gaaggaaatg attcatcgaa tcgatcctca agcctttata actattacag aagcccatga
     2281 aatcctagga gaaggcttca cctttgaaaa agaataaaaa gaggtaatgt cgtgacctca
     2341 aaagttagac taaatcatct atcttttggg ttacagacaa cctctttttt gttttattta
     2401 ctcaagctct taagaccaat tccgagttac ttcttcatca gcctttaact gatccactaa
     2461 ttggtcaact gagtcaaatt tggtcatatc acgaatgcga tcaagccaat aaaccatgac
     2521 ggtttcccca taaatatctt gattaaaatc aaaaatattg acttcaaaac gtgcttcttc
     2581 tccatcaaag gtcacatttt tcccgacact agccatagca cgatacttct gtctttgaat
     2641 ctcaacatca acgacataaa cgccatctgc tggcatataa gtacggtcta aaagcactaa
     2701 attcgctgtc ggataaccaa ttgtacgacc acgagcatta ccatgaacta ccatacctct
     2761 tgatggaagc ggtgccccca aaagttttcc tgcttctttc acatttccat ctaaaatagc
     2821 ttgacggata cgagttgaac taatctttcc tttctcatct tctacaggtg gaacaatgat
     2881 aacttctcca tcaaagtaat cctttaaatc ttctgctgtt tttttgtcag aaccaaatgt
     2941 ataatcaaaa cctgcaacaa taattttggc attcatagcc ttgatataag ttgcaaagaa
     3001 ttcttgtgca gtaagactag cgaattgact actaaaatca aggagatata attcttctac
     3061 accttcgcgc tttaattttc tttcacgttc agcagggttc aaaatatgca aaaacaaatc
     3121 tggatgataa ggctctaaag cgatctttgg agattcatta aaggtcataa cgacgatagg
     3181 taataaatcc tttctcgcag ccttgttggc aacacgaaat aattcttgat gccccttatg
     3241 tatgccatca aaatagccga gaacaacgac tgaatcagat ggtgtgccaa tatctttttg
     3301 gttttttata ggaatagtaa taatcataaa ataattatat catagcgata gctatttctg
     3361 gaacagaaaa tctgaaatgt tgtttttttc acatgaagtg tacctgtttt caaaaagcac
     3421 tttattctat cgttgcttaa ctatgaactt tgcaatattc ttctcaaaaa cttgtaggac
     3481 atcttcaaaa ttttgcaagg agtgattaga cttgttcggt aaccataaag tgtcatacta
     3541 tgttatgtat gaaaaagcaa tgcaactaac tcctgagaac tttaaattac taattggtgc
     3601 cgaaaaggta gaatttagaa tcgaggtaca cctatggctg taaaatttac aaaatgagac
     3661 aacttgggca agatgtttga agaatttcct aaactcccat ctagtgtgct tcagactagg
     3721 ctatttttct ctccatctgt tagcttggat tctcagaccg tttcagctaa agaatatctt
     3781 ttcccttatc agaaggaacg gctcaagcca ttcagacaag tgaagggacg acaagccaat
     3841 atttgaaacc agatagcagt tcttatagtc aattgaaata aaatctgaag aaatcgagta
     3901 ggaaactcat atcaattttt aacagtgttc tattccagat tcatactcaa tgaaaattaa
     3961 agtgcaaact aggaagttag ccgcaggctg tacttgagta cggcaaggtg aaattgacgt
     4021 ggtttgaatt tgattttcga agagtatcaa tccactatac atattaaaaa gtatgggtat
     4081 caaaataaaa aacaggctct ccgaaaactc ggaaagcctt attttaagct gtttctagct
     4141 tcctcacctt tacatttcaa ggttcgggat aaaaggttca atggaccttt ttatttagcg
     4201 attgggtaaa cagaaacttg ttttttatcg cgtcctttac gttcaaagcg tactacgcct
     4261 tcaactttag cgaacaaagt atcgtctcca ccacgaccaa cgtttacacc tggatagatg
     4321 tgtgtaccac gttgacggta aaggattgat ccacctgtta cagtttgtcc gtcagctgct
     4381 ttagctccaa gacgttttgc ttgtgaatca cgtccgtttg atgtagaacc tccacctttt
     4441 ttgtgggcga aaagttgcaa gttgttaaga gtcattttta acataatgtt ttcctccgtg
     4501 ttagttttct gtgataactc tggtttggac gaactcagaa tagttctccg ataagtttgc
     4561 catacctaag aaaaatgatt caaagaataa ctgggtcatt tctctctggt gtgaaggaag
     4621 atcttttggt atttcaacca tcagatagcc accttcatct tcgtttaatt ctaggattgg
     4681 ttcatagcct gcaaatttct caatagaatt gataaagtta atggcaagcg tagaaaccga
     4741 tgcacacacg acatctaagc cgtattcgcc actctcggcg tgtccagtaa tttccgcact
     4801 cctcagctcg ccatcttcgg ctctctcaaa gactgcctgt atcatgtgtt ctccttaaaa
     4861 ttaagcgttg attgcgttga tgacaacttt tgtatatggt tgacggtgac cttgtttacg
     4921 gtggctacct tttttaggtt tgtacttgta agtaaccact ttcttttgtt ttccttgttt
     4981 ttcaacagtt ccaactacag tagctccagc aacaagtgga gttccgacaa cagtgttttc
     5041 accaccaaca agaacaactt cgttaaaagt aacttcttga ccagcttcaa cgttcaattt
     5101 ttcaacgtaa actgcttgac caacttcaac tttaacttgt tttccgccag ttttgataat
     5161 tgcgtatgtg ctcattatgc acctcctatg atttttatgg gtttccccga ttttttgtga
     5221 agactcgcct agcatcgtgg gacgaaccac ttaaacttga ataatcaagc aacgatgttc
     5281 gtgcggttgc acaggatcgt gcatagtcaa ctcttcaagt atagcatatc tcctattttc
     5341 ttacaagtaa taacacctaa aatcaagctt tttcttttac ttttttctgc caagaggcaa
     5401 aaagcatgct gaggtaaaaa acgctcatca taataggaac accaagaatg gtcttttcat
     5461 gatagaaaat cgtcaaatag gctgaaaaga caacgccaag gacaaaacta ctaagcaggc
     5521 taacaaatat gaatccttca cgcaaaaaag gagtgtgctt ggttcggaaa taatctccaa
     5581 aagccagcat ggtccgtttg atattccctg tcataaaagc gttattatag gcaatacccg
     5641 acacttctcc aaaagcagtt gtcaccagtc ccatacagaa ggccaagggc ggcactagat
     5701 agatattatc cacagtttgc ggcacaaaag caataatgat tgataagatt gccaagggaa
     5761 tcaaggacag aataggtttt ttcacaattc tcaatttttc cttataaatc gttaataaaa
     5821 agactcccat cataaacgct agcaaggtga gaaccttgtc cctaacatcc gaaacattat
     5881 ttttaattaa ttctactgaa agaaagacaa catttccagt ttgtccagct acaagggtat
     5941 tcccgcgaac aataaaagtg taagcatcca catatccagc acaaaacgtc aaaaaaagtg
     6001 ctaacctttt agactgacgt gatatttttc ttataggtaa taacctcatt ttacctccca
     6061 ttgtattttc tcttagaaat attgtaccat tttctttcta aaaaatcgta ggctaccatt
     6121 tagattttac tattagcata aaaataataa tagacaacta tttatccaaa aatagataga
     6181 tgtaacatgt ttgcaaacaa agcatacgaa cctttagtaa aatcatttcc atgaaactag
     6241 aatagagccc tcttagcaaa aatcattatt ttaatttatt tctaatcact ccttgacata
     6301 aataactctc accaataaaa gactatgtct taaaaaaatg gtataataaa atcaatactt
     6361 gggcttgatg gctatgctac taataacaat taggagagaa aatcaggcac ttgttaacaa
     6421 caaggattat ccccttgaga tgaaaggaac tttagaaatc ttatgatgaa catgcaaaac
     6481 atgatgcgtc aagcacaaaa acttcaaaaa caaatggaac aaagccaagc tgaacttgct
     6541 gctatgcaat ttgttggcaa atctgctcaa gatcttgtcc aagcgacctt aactggcgat
     6601 aagaaagttg tcagcattga tttcaatcca gctgtcgttg acccagagga ccttgagact
     6661 ctttctgata tgaccgttca agccatcaac tctgctcttg aacaaatcga tgaaactacc
     6721 aagaaaaaac tgggtgcttt cgctgggaaa ttacctttct aaaaacaagg agctagaaca
     6781 atgcttgtcg ataacaaagg ctaagaaagg tgcaaaaatg actctataat atttgtagtg
     6841 ggtaaatcca ctatagatat tatggagcct attttattgt agaaaaaaag tcccataaga
     6901 tctataatga aaagcga
//
LOCUS       DAVKMU010000073         6857 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKMU010000073 DAVKMU010000000
VERSION     DAVKMU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.073"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..465
                     /locus_tag="VW343_001923"
                     /pseudo
     CDS             1..465
                     /locus_tag="VW343_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            632..832
                     /locus_tag="VW343_001924"
     CDS             632..832
                     /locus_tag="VW343_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756430.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            857..1273
                     /locus_tag="VW343_001925"
     CDS             857..1273
                     /locus_tag="VW343_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756431.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLDVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1504..2211
                     /gene="ccdA2"
                     /locus_tag="VW343_001926"
     CDS             1504..2211
                     /gene="ccdA2"
                     /locus_tag="VW343_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9756432.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2222..2779
                     /gene="sdbB"
                     /locus_tag="VW343_001927"
     CDS             2222..2779
                     /gene="sdbB"
                     /locus_tag="VW343_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9756433.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2900..4291
                     /locus_tag="VW343_001928"
     CDS             2900..4291
                     /locus_tag="VW343_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9756434.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4434..5351
                     /gene="adcAII"
                     /locus_tag="VW343_001929"
     CDS             4434..5351
                     /gene="adcAII"
                     /locus_tag="VW343_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9756435.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5359..>6857
                     /locus_tag="VW343_001930"
     CDS             5359..>6857
                     /locus_tag="VW343_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9756436.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT         2124 a         1247 c         1432 g         2054 t
ORIGIN      
        1 atagagatag acttaaaaac tatcttactg acaatccaga cgcttatttg actgaaatag
       61 cttctgaatt tggctgtcat ccaactacca tccactatgc gctcaaagct atgggctaca
      121 ctcgaaaaaa gaaccacacc tactatgaac aagacccaga aaaagtagcc ttatttctta
      181 agaattttaa tagtttaaag cacctagcac ctgtttagat tgacgaaaca ggattcgata
      241 cttattttta tcgagaatat ggtcgctcat taaaaggtca gttaagaaga ggcaaagtat
      301 ctggaagaag atatcagaga atttctttgg ttgcaggtct aacaaatgaa tggtgagtta
      361 atcgctccaa tgacttacga agagacgatg acgagcgact tttttgaagt atggtttcag
      421 aaatttctct taccaacatt aaccacacca tcggttatta tagtaaaatg gaataagaac
      481 aggacaaatc gatcaggaca gtaaaatcga attctaacaa tgttttagaa gtctatatgt
      541 actattctag tttcaatata ccataactgc tcctagtcaa cctgcaagtc ctgtcccagc
      601 tccaagctca gctccgactc aagatcctag agtggtagca agcgcttcgg ctagctcaac
      661 gagcacacaa gctcaagagc aagttgacaa gtctgaactt cgtgccttga gtcaagagtt
      721 agaccaacgc ttgaaagctt tggcaacagt atctgatcca aaaatcgatg caaccaaggc
      781 tgtcctccta gatgctcaaa aagctccgga agatagtgcc ttgacagagt aaggtctgca
      841 tactgcagta gagtctgtga aggctgcctt ggactctctc aaagatgtga aagcaaatgc
      901 tagcgacagc aagcctgcac aggacaagaa ggatgcaaaa caaggaacgg aagatagtaa
      961 ggattcagat aagatgactg aaacaaactc agttccggca ggagtgattg tggtcagtct
     1021 acttgccctc ctagacgtga ttgccttctg gctgattcgc cgtaagaaag agtcagaaat
     1081 ccagcaatta agcacggaat tgatcaaggt tctaggacag ctagatgcag aaaaagcgga
     1141 taaaaaagtc cttgccaaag cccaaaacct tctccaagaa acccttgatt tcgtgaaaga
     1201 agaaaatggc tcagcagaga cagaaactaa actagtagag gagcttaaag caatccttga
     1261 caaactcaag taaaacatga tttatgaaat gtcagaccta aaagacattt tcgttcacaa
     1321 aagcagtgac gagtaatcct tgtcgttgct ttttggctat tttgggggat aagatccgcc
     1381 ttgactagca aaagaaaaga ttggtccaat agtgcccgat acgattttgc gtagcagaaa
     1441 gcttacaaat aaaattgaat atagtaaaat aggattagaa ttattaagaa agttggttct
     1501 ttattggaaa cgatagtatt tttaatctct gtttttctag caggtgtttt atcctttttt
     1561 tctccttgta tttttcctct tctgccagtc tatgctggga ttttattgga tgatcaagaa
     1621 agtgcaaaaa gcttttcttt gtttgggaga aaggttctct ggtcaggctt gattcgaaca
     1681 ctttgcttta tcgctggtat ctctctcatt ttctttattc taggctttgg tgctggttac
     1741 tttggtcata ttctctatgc aaattggttt cgatatgtca tgggagctat tattatcatt
     1801 ttgggtcttc accagatgga aatttttcat ttgaagaaat tagaagttca aaaaagtttt
     1861 acctttaaaa aatcagattc taatcgttat tggtcagctt ttttacttgg tattaccttt
     1921 agctttggtt ggacaccttg tattggtcca gttttaagtt ctgttttagc acttgcggct
     1981 tctggaggca atggtgcttg gcaaggagcg atttatactc tcatttacac tctgggcatg
     2041 gcccttcctt tcttggtctt ggcactagct tcaggtctag tcatgccata ttttagtaaa
     2101 atcaagcgtc atatgatgct actaaagaaa attggtggtt tcctcattgt tttaatggga
     2161 attttgttac tattaggaca agtaaatgtt ctagctggaa tttttgaata aaggaggaca
     2221 aatgaaaaaa gtaatgtttg ctggcttaag tctcttgtca ttagttgtat tgatggcctg
     2281 tggtgaggaa gaaactaaaa agactcaagc agcacaacag ccaaaacaac aaacgactgt
     2341 acaacaaatt gctgttggaa aagatgctcc agacttcaca ttgcaatcca tggatggcaa
     2401 agaagttaag ttatctgatt ttaagggtaa aaaggtttac ttgaagtttt gggcttcatg
     2461 gtgtggtcca tgcaagaaaa gtatgccaga gttgatggaa ctagcggcga aaccagatcg
     2521 tgatttcgaa attcttactg tcattgcacc aggaattcaa ggtgaaaaaa ctgttgagca
     2581 attcccacaa tggttccagg aacaaggata taaggatatc ccagttcttt atgataccaa
     2641 agcaaccacc ttccaagctt atcaaattcg aagcattcct acagaatatt taattgatag
     2701 ccaaggaaag attggaaaga ttcaatttgg tgctatcagt aatgcggatg cagaagcagc
     2761 atttaaagaa atgaactaga atcgataaaa tctgattaca agatgtagtc agattttttt
     2821 agaaaaacat tttataaata ttcacatatc tcctatattt atggtaaaat agaattatca
     2881 gtttattttg gagtcaaaga tgaatatatt tagaacaaag aatgttagtt tagataaaac
     2941 agagatgcat aggcatttga agttatggga tttgattttg ctgggtatcg gagccatggt
     3001 agggacaggc gtctttacaa tcacaggtac tgcagctgca acacttgctg gcccagccct
     3061 agtgatttca atcgttattt ctgccttgtg tgtgggatta tcagccctct tttttgcaga
     3121 atttgcctcg cgagtacccg ctacaggagg tgcctatagt tacctctatg ctatcttagg
     3181 agaattccct gcctggttgg ctggttggtt aaccatgatg gagttcatga cagccatatc
     3241 aggcgtagct tcgggttggg cagcttattt taaaggcttg ctctctcaat acgggatagc
     3301 ctttcctcag gctttaaatg gtacctttaa tcctcaagca gggacatttg ttgatttatt
     3361 gcctattctt gtcttggtct tggtgacttc gcttgtttta ctaaatgcca aggcagcctt
     3421 acgctttaat tcgattctag ttattttgaa attttccgct ttagctctct ttgttttagt
     3481 aggaatttgg aatattaagt ttgataattg gagcaatttt gctccttacg gttttggaca
     3541 aatctatgga gctagcactg gtattatggc tggtgcgtcc ttgatgttct tcggtttttt
     3601 gggatttgaa tccatctcta tggctgtaga tgaagtcaag actcctcaaa aaaatattcc
     3661 tagagggatt gtcttatcgc tttctatcgt aaccattctc tatgccttgg tgacccttgt
     3721 tttgactggt gtggttcact atagtcattt aaatgtcgat gatgccgttg cctttgccct
     3781 tcgtagtgtt gggattagtt gggcagccaa ctatgtgtca ttagtggcta tcttgacctt
     3841 gattacagtt tgtatttcga tgacctatgc cctatcgcgt atgatttaca gtttagcacg
     3901 tgacggctta gtgcctgctg cctttaagga actgacgaag actagcaaga taccaaagaa
     3961 tgctactatc ttgacaggtc tagcttcagc agtagcagca ggaatgttcc cactagccag
     4021 tattgcagcc tttttaaata tttgtacctt agcctacttg atcatgctgg cttatggtct
     4081 gattcgctta cggaaagaaa aaggaatgcc caaagctggg gaatttaaaa caccattggt
     4141 acctctgtta ccaattttat cgatcatcat ttgtttatcc ttcatgttac agtataatat
     4201 gaacacctgg attgctttcc tagttgcgtt gttagtagga agtattattt acttcactta
     4261 tggctataag cattctacca tagaagaata aagtgaaagt ctattgagtt gttgacactc
     4321 agtagatttt tatatgtgaa agaaatttca aattttttct aaaaatatct tgacagatta
     4381 aattttcagg agtagaatat ttactagtta attaaatggt taaggagttg ttcatgaaga
     4441 aacaaaattt atttttagtc ctgttaagtg tctttctttt gtgtttgggg gcttgtggtc
     4501 aaaaggaaag tcagacagga aaggggatga aaattgtgac cagtttttat cctatctacg
     4561 ctatggttaa ggaagtatct ggtgacttga atgatgttcg gatgattcag tcaagtagtg
     4621 ggattcactc ctttgaacct tcggcaaatg atatcgcagc catctatgat gcagatgtct
     4681 ttgtttacca ttctcataca ctcgaatctt gggcaggaag tctggatcca aatctaaaaa
     4741 aatccaaagt gaaggtctta gaggcttctg agggaatgac cttggaacgt gtccctggac
     4801 tagaggatgt ggaagcaggg gatggagttg atgaaaaaac gctctatgac cctcacacat
     4861 ggctagatcc tgaaaaagct ggagaagaag cccaaattat cgctgataaa ctttcagagg
     4921 tggatagtga gcataaagag acttatcaaa aaaatgcgca agcctttatc aaaaaagctc
     4981 aggaattgac taagaaattc caaccaaaat ttgaaaaagc gactcagaaa acatttgtaa
     5041 cacaacatac agccttttct tatctagcga agagatttgg gcttaatcaa cttggtattg
     5101 caggtatctc tcctgaacaa gaaccaagtc cacgacaact aacagaaatt caggaatttg
     5161 ttaagaccta taaggttaaa acgattttta cagaaagtaa cgcttcttca aaagtagctg
     5221 aaactcttgt caaatcaaca ggtgtgggtc ttaaaactct gaatccttta gagtcagacc
     5281 cacaaaatga caagacctat ttagaaaatc ttgaagaaaa tatgagtatt ctagcagaag
     5341 aattaaagtg aggaaagaat gaaaatcaat aaaaaatatc tagcaggttc agtggcagtc
     5401 cttgccctaa gtgtttgttc ctatgaactt ggtcgtcacc aagctggtca ggttaagaaa
     5461 gagtctaatc gagtttctta tatagatggt gatcaggctg gtcaaaaggc agaaaatttg
     5521 acaccagatg aagtcagtaa gagagagggg atcaacgccg aacaaattgt tatcaagatt
     5581 acggatcaag gttatgtgac ctctcatgga gaccattatc attactataa tggcaaggtt
     5641 ccttatgatg ccatcatcag tgaagaactt ctcatgaaag atccgaatta tcagttgaag
     5701 gattcagaca ttgtcaatga aatcaagggt ggctatgtga ttaaggtaga cggaaaatac
     5761 tatgtttacc ttaaagatgc agcccatgcg gacaatattc ggacaaaaga agagattaaa
     5821 cgtcagaagc aggaacgcag tcataatcac aactcaagag cagataatgc tgttgctgca
     5881 gccagagccc aaggacgtta tacaacggat gatgggtata tcttcaatgc atctgatatc
     5941 attgaggaca cgggtgatgc ttatattgtc cctcacggca atcacttcca ctatattcct
     6001 aagagtgatt tgtctgccag tgaattagct gctgcccaag cctattggaa tgggaagcag
     6061 ggatctcgtc cttcttcaag ttctagtcat aatgcaaatc cagctcaacc aagattgtca
     6121 gagaaccaca atctgactgt cactccaact tatcatcaaa atcaagggga aaacatttca
     6181 agccttttac gtgaattgta tgctaaaccc ttatcagaac gccatgtaga atctgatggc
     6241 ctagtatttg atccggcaca aataacatat cgtacagtca atggtgttgc tgtaccgcac
     6301 ggagaccatt atcactttat tccttattca caactgtcac ctttggaaga aaaattggct
     6361 cgtatgattg ctgttaaggg acaaaatggt gcagtcttgc caggaatgca ttatttgaaa
     6421 ccagcaccaa aaccgcaagt tcaaccatca actgagaaaa aacaaactga ttttgcagta
     6481 gaacaggttg ttagaaaagt tggagaaggc tatgttgtgg aaattgctgg tgtcagtcac
     6541 tatgtttttg ctaaagactt ggctaaggat aagattgatg ctattgaaaa tctcttgtct
     6601 aagaaaactc aagagacaca tgctctagtt gctaaaaaag aaaatgtcgc tcctcgtgac
     6661 caagaatttt atgataaagc ttacaatctg ttaacccagg ctcataaagt cttgtctgaa
     6721 aataaaggtc gtacttctga tttccaagcc ttagacaaat tagcagaacg cttgaataat
     6781 gaatcttcta ataaagtaaa attggtagat gatttattgg cattcctagc accaattacc
     6841 cacccagaac gccttgg
//
LOCUS       DAVKMU010000074         6846 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKMU010000074 DAVKMU010000000
VERSION     DAVKMU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.074"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..752)
                     /locus_tag="VW343_001931"
     CDS             complement(<1..752)
                     /locus_tag="VW343_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9756437.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNTPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQI
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHAQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(749..2074)
                     /locus_tag="VW343_001932"
     CDS             complement(749..2074)
                     /locus_tag="VW343_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9756438.1"
                     /translation="MDFFLLVDLILYFLIISHSYRLICKDQINRKELFFFGAYTLLTE
                     IVLDFPFYLLYLDGLGIATFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYYGLFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRVYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKRKFE"
     gene            complement(2087..2227)
                     /gene="comC"
                     /locus_tag="VW343_001933"
     CDS             complement(2087..2227)
                     /gene="comC"
                     /locus_tag="VW343_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9756439.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIQGGEMRKMNEKSFNIFNFFNFFR
                     RR"
     gene            complement(2392..2465)
                     /locus_tag="VW343_001934"
     tRNA            complement(2392..2465)
                     /locus_tag="VW343_001934"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2429..2431),aa:Arg,seq:tct)
     gene            complement(2509..2988)
                     /gene="rlmH"
                     /locus_tag="VW343_001935"
     CDS             complement(2509..2988)
                     /gene="rlmH"
                     /locus_tag="VW343_001935"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9756440.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3171..4352
                     /locus_tag="VW343_001936"
     CDS             3171..4352
                     /locus_tag="VW343_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9756441.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            4410..5168
                     /locus_tag="VW343_001937"
     CDS             4410..5168
                     /locus_tag="VW343_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9756442.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIIYFSNQEEYSRIINSLK"
     gene            5381..6742
                     /gene="dnaA"
                     /locus_tag="VW343_001938"
     CDS             5381..6742
                     /gene="dnaA"
                     /locus_tag="VW343_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9756443.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2374 a         1221 c         1086 g         2165 t
ORIGIN      
        1 cacttttgag attttttctc taaaatatct tttaattttc taatttttaa tcttgaaata
       61 ggacaacgat ggtcttcata gaaaacaatt tctaagtttt ttcgatcaat ttctctgata
      121 ttgcctatat ttaccaaaaa tgacttgtga ggagaataaa atcgctgagc atgtttgtcc
      181 ttttcctgaa tatctgtcat ggtaccataa aactcttttg caaaattctt accaataatg
      241 cgcaatttat gagagactcc tgtcgtttca atatacaaaa tatcatggta aggaattttt
      301 aaatcatttc ccttgtaatt gtagtcgaaa taatctacaa catcttcatt ttcaagtaac
      361 atactcttcg tgtagaagat attttgctca attcgcttct taaacatctc atcgttgata
      421 tccttatcaa caaaatctag ggctgatatc tggtatttat aggtcagagt cgcaaactct
      481 gatcgactgg tgataaagac gataatagcg taaggattgt aatgacgaat gagttgagca
      541 acttcaaatc cctttttctc aattccatga atatcgatat ctaggaaata aagttgattt
      601 acttcatcat tttcaatgta ttcttcaaat tcacggactt ttcccgttgt cttgtatgat
      661 attggagtat tcgattcttt cgaaatttca tccaatattc tctctagtct cacttgatgt
      721 tcaataacat cttctaaaat taaaactttc attcaaattt cctcttaaat ctaatgattt
      781 gtctaaatgt actgccttcc atctctgttt ctaaaataat gttgttgtac ttatctagta
      841 gttctttcac attatttaat ccgactccgc gatttcttcc cttagtggag aatcctaagg
      901 caaatagatc tcctgaagga gtcatcgtca ttttacatga attctgaatc acaataactg
      961 tttcagtttc catcttaata actgctactt ccatctgctt tttatagcta tcagccgatc
     1021 cttcgacagc attgttcaat aaaacgctca tgatacgcac taaatccaat agttcaattg
     1081 gaagcttggt aatcgtatct tttacttcca gtgtaaactc tacaccatta tttcgagcat
     1141 agacaattga ctgagcaacc aaacttcgta aagctgaatc ttctatgttg ttcaaatcaa
     1201 agtaagtgta cttatctgaa cgtagtttat gatttgcttt gaccaaaact tcattgtaaa
     1261 ctctgtcaat ttcctgtaaa ttaccactgt caattgccat ctgcatgctg acaagcattc
     1321 cagcataatc atgtcgaaaa ccacggattt cattatacag accgacaatt tcatctgtgt
     1381 aattctgtaa atgtttctgt tcaaatttct tctgcttcaa agcaatctct ttctccattt
     1441 gaactttatg agaattcatt gcaaagaagg tcaaaaggag agagataaag acaatagatg
     1501 acaaaatact tccaaaacta ttcaaatgtt taatcgtact taccatatct gaaacgaaag
     1561 atacaatatg tagcaatagt aaagcaaaaa atactttttt caagaaagga taaaggtagt
     1621 ccttgtcaaa ataggctagt tccaaatgga aataataaat gatttttaat gtaacaaaat
     1681 aggtcaacac cgtcacaacg aaaaagaata ggccataata ctgtaaaaca aaattgtctc
     1741 ctgttataga agagaaagtt acggacagaa agttatgagt gctctcatat aaaagagata
     1801 gtagtaaact tagaaatagt cctctatccc tctcatactg tttcatccat cgaaaatagg
     1861 aatataggcc caaaggaaat aaaaatgttg caatccctaa cccatctaaa tatagaagat
     1921 agaagggaaa atcaagtact atttctgtta gtaatgtata agcaccaaaa aagaataatt
     1981 cttttctatt tatttgatct ttacaaatta aacggtaact atgactaata attaaaaaat
     2041 ataatatcaa atctactaat aagaaaaaat ccattctgct actcctttat cttcttctaa
     2101 agaaattaaa gaaattaaag atattaaagg acttttcatt cattttcctc atctccccac
     2161 cttgaatatt ttgcaagtct ttttccttca aagctacaaa ctgttccaat ttaactgtgt
     2221 ttttcataat aaaatctcct aaaatgtttt ttcttgtaag ctaacttaca aaaaccatta
     2281 tacaaaatgg aatttcgttt tagataaaat tctctcaact gtcatttttt tctcccaaag
     2341 tgtacttttt taagaaaaaa gccgggaaaa ttcccagctt tgctattata ttgatcccag
     2401 caggattcga acctgcgacc gttcgcttag aaggcgaatg ctctatccag ctgagctatg
     2461 agacctaaca caattattct accaaaaatt caattaaaag tcaattttct atttatggta
     2521 ggggaatccc tgctgaatcg taaaagcgcg atagatttgt tcaacaagaa ctagtctcat
     2581 taactgatga ggcaaggtta ggcgaccaaa actgacagaa agattggctc tattttttac
     2641 agatgatgat aatcctaaac ttcccccaat aataaaagta agagtagaaa atccttttat
     2701 agaagtttct tctaactgct tactaaattc ttctgagaag aaagttttcc cttcaatggc
     2761 taacacaata acgaaatcac ggtcagcaat ttttgataaa attctctgac cttctatttc
     2821 taaaatcttt tgattttctg attcactggc cttatctggt gttttttcat ctgataactc
     2881 aatcatttca aacttagcaa atctagaaat tcgttttgaa tactctgcga taccatcttt
     2941 taaatacttt tctttcagtt tcccaactgt tacaacttta attttcatga ctctattcta
     3001 acatattctc tattttttca catcttattc acaaaataaa aaatagattt caattaagaa
     3061 aatcacagtt tcaaaagagt tatccacagt ttgtgtaaaa cttttgtgtt taagttataa
     3121 ttaagctagt cagtttatac tttcagtaat tcaaacatat ggaggcaaat atgaaacatc
     3181 taaaaacatt ttacaaaaaa tggtttcaat tattagtcgt tatcgtcatt agctttttta
     3241 gtggagcctt gggtagtttt tcaataactc aactaactca aaaaagtagt gtaaacaact
     3301 ctaacaacaa tagtactatt acacaaactg cctataagaa cgaaaattca acaacacagg
     3361 ctgttaacaa agtaaaagat gctgttgttt ctgttattac ttattcggca aacagacaaa
     3421 atagcgtatt tggcaatgat gatactgaca cagattctca gcgaatctct agtgaaggat
     3481 ctggagttat ttataaaaag aatgataaag aagcttacat cgtcaccaac aatcacgtta
     3541 taaatggcgc cagcaaagta gatattcgat tgtcagatgg gactaaagta cctggagaaa
     3601 ttgtcggagc tgacactttc tctgatattg ctgtcgtcaa aatctcttca gaaaaagtga
     3661 caacagtagc tgagtttggt gattctagta agttaactgt aggagaaact gctattgcca
     3721 tcggtagccc gttaggttct gaatatgcaa atactgtcac tcaaggtatc gtatccagtc
     3781 tcaatagaaa tgtatcctta agatcggaag atggacaagc tatttctaca aaagccatcc
     3841 aaactgatac tgctattaac ccaggtaact ctggcggccc actgatcaat attcaagggc
     3901 aggttatcgg aattacctca agtaaaattg ctacaaatgg aggaacatct gtagaaggtc
     3961 ttggtttcgc aattcctgca aatgatgcta tcaatattat tgaacagtta gaaaaaaacg
     4021 gaaaagtgac gcgtccagct ttgggaatcc agatggttaa tttatctaat gtgagtacaa
     4081 gcgacatcag aagactcaat attccaagta atgttacatc tggtgtagtt gttcgttcgg
     4141 tacaaagtaa tatgcctgcc aatggtcacc ttgaaaaata cgatgtaatt acaaaagtag
     4201 atgacaaaga gattgcttca tcaacagact tacaaagtgc tctttacaac cattctatcg
     4261 gagacaccat taagataacc tactatcgta acgggaaaga agaaactacc tctatcaaac
     4321 ttaacaagag ttcaggtgat ttagaatctt aattgacatc tatgtaaaga aagctttaca
     4381 taagagaaaa gatgtgttag tgtagaatca tggaaaaatt tgaaatgatt tctatcacag
     4441 atatacaaaa aaatccctat caaccccgaa aagaatttga tagagaaaag ctagatgaac
     4501 tagcacagtc tatcaaagaa aatggggtca ttcaaccgat tattgttcgt caatctcctg
     4561 ttattggtta tgaaatcctt gcaggagaga gacgctatcg ggcttcactt ttagctggtc
     4621 tacggtctat cccagctgtt gttaaacaga tttcagacca agagatgatg gtccagtcca
     4681 ttattgaaaa tttacagaga gaaaatttaa acccaataga agaagcacgc gcctatgaat
     4741 ctctcgtaga gaaaggattc acccatgctg aaattgcaga taagatgggc aagtctcgtc
     4801 catatatcag caactccatt cgtttacttt ccttgccaga acagattctc tcagaagtag
     4861 aaaatggcaa actatcacaa gcccatgcgc gttccctagt tgggttaaat aaggaacaac
     4921 aagactattt ctttcaacgg attatagaag aagatatttc tgtaaggaag ttagaagctc
     4981 ttctgacaga gaaaaaacaa aagaaacagc aaaaaactaa ttatttcata caaaatgaag
     5041 aaaaacagtt aagaaaacta ctcggattag atgtagaaat taaactatct aaaaaagaca
     5101 gtggaaaaat cattatttat ttttcaaatc aagaagaata tagtagaatt atcaacagcc
     5161 tgaaataagg ctgttctttt atttttttat ctcacaaggt tatccactat gtttttcgat
     5221 aaaaagctta ataaatcaat aatttcttct tttatcccca acctgtggat aaagtttagt
     5281 aacattgtgg attatttttc acagcttgtg gaaaattctt gctatctatg gtaaaatatc
     5341 tctagtatta aacttttaaa tagtaaagga ggaaaaagga ttgaaagaaa aacaattttg
     5401 gaatcgtata ttagaatttg cacaagaaag actgactcga tccatgtatg atttctatgc
     5461 tattcaagct gaactcatca aggtagagga aaatgttgcc actatatttc tacctcgctc
     5521 tgaaatggaa atggtctggg aaaaacaact aaaagatatt attgtagtag ctggttttga
     5581 aatttatgac gctgaaataa ctccccacta tattttcacc aaacctcaag atacgactag
     5641 ctcacaagtt gaagaagcta caaatttaac tctttatgac tatagtccaa agttagtatc
     5701 tattccttat tcagatacgg gattaaaaga aaagtatacc tttgataact ttattcaagg
     5761 ggatggaaat gtttgggctg tgtcagccgc tttagctgtc tctgaagatt tggctctgac
     5821 ctataaccct ctttttatct atggaggacc aggccttggt aagactcact tattaaacgc
     5881 tattggaaat gaaattctaa aaaatattcc taatgcgcgt gttaaatata tccctgccga
     5941 aagctttatt aatgactttc ttgatcacct aagacttggg gaaatggaaa agtttaaaaa
     6001 gacctatcgt agtcttgatc ttttgttaat cgatgatatc cagtcactca gcggaaaaaa
     6061 agtcgcaact caggaagaat ttttcaatac ctttaacgcc cttcatgaca agcaaaaaca
     6121 gattgtccta acgagtgatc gtagtccaaa acatctagaa gggctcgagg agaggcttgt
     6181 cacgcgtttt agttggggat tgacacaaac tatcacaccc cctgactttg aaacacgtat
     6241 tgccatttta caaagtaaaa cggaacattt aggctacaat ttccaaagtg atactctaga
     6301 atacctagct gggcaatttg attcaaatgt tcgagatctt gagggagcca tcaacgacat
     6361 cactttaatt gccagagtaa aaaaaatcaa ggatatcact attgatattg ctgcagaagc
     6421 cattagagcc cgcaaacaag atgttagcca aatgctcgtc atcccaattg ataaaatcca
     6481 aactgaagtt ggtaactttt atggtgttag tatcaaagaa atgaagggaa gtagacgcct
     6541 tcaaaatatt gttttggccc gtcaagtagc catgtattta tctagagaac taacagataa
     6601 tagtcttcca aaaattggga aggaatttgg gggaaaagat cataccacag tcattcatgc
     6661 ccatgccaaa ataaaatctt tgattgatca agacgataat ttacgtttag aaattgaatc
     6721 aatcaaaaag aaaatcaaat aatttgtgga taacttttag ttttttatct tttttatcca
     6781 cattttttaa acaagctaaa aaacttgata ggacttgttt aaaggctgtt ttccacagat
     6841 ttcaca
//
LOCUS       DAVKMU010000075         6832 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKMU010000075 DAVKMU010000000
VERSION     DAVKMU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.075"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            84..6503
                     /locus_tag="VW343_001939"
     CDS             84..6503
                     /locus_tag="VW343_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9756444.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     AENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKEENSNK
                     SQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGSKAIKELSSLKNTKVLYTYDRIF
                     NGGAIETTQDNLNKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHF
                     IRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKEKSSF
                     TIDRNISTIRDFENKDLKKLIKKKYKEEDDFVTGGKRTVELDYKYDDKGNITAYEDES
                     ALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEIKATK
                     YDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDDRIKAETKIRMPEKNKETKAEY
                     PYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKV
                     TTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLM
                     SALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEA
                     IKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVN
                     ESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYL
                     QTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFD
                     GEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSE
                     LKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYV
                     INLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDH
                     SQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLG
                     LKKKNQD"
BASE COUNT         2704 a          919 c         1326 g         1883 t
ORIGIN      
        1 ttagtaaaac tttttataac aaaggctagc aaagttaaag tttattatct attggaagtt
       61 atataaatat gtaaggaatt aaaatgaaaa aaagtacagt attgtcatta accacagctg
      121 cagttatttt agcagcctat gcccctaatg aggtagtctt agcagacaca tctagctctg
      181 aagatgcttt aagcatctct gataaagaaa aagtagcaga aaataaagag aaacataaag
      241 atattcataa tgctatagaa acttcaaagg atactgaaga aaagaaaaca acaattattg
      301 aggaaaaaga agttgttagt aaaaatcctg tgatagacac taaaactagc aatgaagaag
      361 caaaaatcaa agaagaaaat tccaataaat cccaaggaga tcatacggac tcatttgtga
      421 ataaaaacac agaaaatccc aaaaaagaag ataaagttgt ctatattgct gaatttaaag
      481 ataaggaatc tggatcaaaa gcaatcaagg aactatccag tcttaagaat acaaaagttt
      541 tatatactta tgatagaatt tttaacggtg gtgctattga aacaactcaa gataatctga
      601 ataaaattaa acaaatagaa ggtattacat cggttgaaag ggcacaaaaa gtccaaccca
      661 tgatgaatca tgccagaaag gaaattggag ttgaggaagc tattgattac ctaaagtcta
      721 tcaatgctcc gtttgggaaa aattttgatg gtagaggtat ggtcatttca aatatcgata
      781 ctggaacaga ttataggcat aaggctatga gaatcgatga tgatgccaaa gcctcaatga
      841 gatttaaaaa agaagactta aaaggaactg ataaaaatta ttggttgagt gataaaatcc
      901 ctcatgcgtt caattattat aatggtggca aaatcactgt agaaaaatat gatgatggaa
      961 gggattattt tgacccacat gggatgcata ttgcagggat tcttgctgga aatgatactg
     1021 aacaagatat caaaaacttt aacggcatag atggaattgc acctaatgca caaattttct
     1081 cttacaaaat gtattctgac gcaggatctg ggtttgcggg tgatgaaaca atgtttcatg
     1141 ctattgaaga ttctatcaaa cacaacgttg atgttgtttc ggtatcatct ggttttacag
     1201 gaacaggtct tgtaggtgag aaatattggc aagctattag ggcattaaga aaagcaggca
     1261 ttccaatggt tgtcgctacg ggtaactatg cgacttctgc ttcaagttct tcatgggatt
     1321 tagtagcaaa taatcatctg aaaatgaccg acactggaaa tgtaacacga actgcagcac
     1381 atgaagatgc gatagcggtc gcttctgcta aaaatcaaac agttgagttt gataaagtta
     1441 acataggtgg agaaagtttt aaatacagaa atataggggc ctttttcgat aagaataaaa
     1501 tcacaacaaa tgaagatgga acaaaagctc ctagtaaatt aaaatttgta tatataggca
     1561 aggggcaaga ccaagatttg ataggtttgg atcttagggg caaaattgca gtaatggata
     1621 gaatttatac aaaggattta aaaaatgctt ttaaaaaagc tatggataag ggtgcacgcg
     1681 ccattatggt tgtaaatact gtaaattact acaatagaga taattggaca gagcttccag
     1741 ctatgggata tgaagcggat gaaggtacta aaagtcaagt gttttcaatt tcaggagatg
     1801 atggtgtaaa gctatggaac atgattaatc ctaataaaaa aactgaagtc aaaagaaata
     1861 ataaagaaga ttttaaagat aaattggagc aatactatcc aattgatatg gaaagtttta
     1921 attccaacaa accgaatgta ggtgacgaaa aagagattga ctttaagttt gcacctgaca
     1981 cagacaaaga actttataaa gaagatatca tcgttccagc aggatctaca tcttgggggc
     2041 caagaataga tttactttta aaacccgatg tttcagcacc tggtaaaaat attaaatcca
     2101 cgcttaatgt tattaatggc aaatcaactt atggctatat gtcaggaact agtatggcga
     2161 ctccaatcgt ggcagcttct actgttttga ttagacctaa attaaaggaa atgcttgaaa
     2221 gacctgtatt gaaaaatctt aagggagatg acaaaataga tcttacaagt cttacaaaaa
     2281 ttgccctaca aaatactgcg cgacctatga tggatgcaac ttcttggaaa gaaaaaagtc
     2341 aatactttgc atcacctaga caacagggag caggcctaat taatgtggcc aatgctttga
     2401 gaaatgaagt tgtagcaact ttcaaaaaca ctgattctaa aggtttggta aactcatatg
     2461 gttccatttc tcttaaagaa ataaaaggtg ataaaaaata ctttacaatc aagcttcaca
     2521 atacatcaaa cagacctttg acttttaaag tttcagcatc agcgataact acagattctc
     2581 taactgacag attaaaactt gatgaaacat ataaagatga aaaatctcca gatggtaagc
     2641 aaattgttcc agaaattcac ccagaaaaag tcaaaggagc aaatatcaca tttgagcatg
     2701 atactttcac tataggcgca aattctagct ttgatttgaa tgcggttata aatgttggag
     2761 aggccaaaaa caaaaataaa tttgtagaat catttattca ttttgagtca gtggaagaaa
     2821 tggaagctct aaactccaac gggaagaaaa taaacttcca accttctttg tcgatgcctc
     2881 taatgggatt tgctgggaat tggaaccacg aaccaatcct tgataaatgg gcttgggaag
     2941 aagggtcaag atcaaaaaca ctgggaggtt atgatgatga tggtaaaccg aaaattccag
     3001 gaaccttaaa taagggaatt ggtggagaac atggtataga taaatttaat ccagcaggag
     3061 ttatacaaaa tagaaaagat aaaaatacaa catccctgga tcaaaatcca gaattatttg
     3121 ctttcaataa ccaagggatc aacgctccat catcaagtgg ttctaagatt gctaacattt
     3181 atcctttaga ttcaaatgga aatcctcaag atgctcaact tgaaagagga ttaacacctt
     3241 ctccacttgt attaagaagt gcagaagaag gattgatttc aatagtaaat acaaataaag
     3301 agggagaaaa tcaaagagac ttaaaagtca tttcgagaga acactttatt agaggaattt
     3361 taaattctaa aagcaatgat gcaaagggaa tcaaatcatc taaactaaaa gtttggggtg
     3421 acttgaagtg ggatggactc atctataatc ctagaggtag agaagaaaat gcaccagaaa
     3481 gtaaggataa tcaagatcct gctactaaga taagaggtca atttgaaccg attgcagaag
     3541 gtcaatattt ctataaattt aaatatagat taactaaaga ttacccatgg caggtttcct
     3601 atattcctgt aaaaattgat aacaccgccc ctaagattgt ttcggttgat ttttcaaatc
     3661 ctgaaaaaat taagttgatt acaaaggata cttatcataa ggtaaaagat cagtataaga
     3721 atgaaacgct atttgcgaga gatcaaaaag aacatcctga aaaatttgac gagattgcga
     3781 acgaagtttg gtatgctggc gccgctcttg ttaatgaaga tggagaggtt gaaaaaaatc
     3841 ttgaagtaac ttacgcaggt gagggtcaag gaagaaatag aaaacttgac aaagacggaa
     3901 ataccattta tgaaattaaa ggtgcgggag atttaagggg aaaaatcatt gaagtcattg
     3961 cattagatgg ttctagcaat ttcacaaaga ttcatagaat taaatttgct aatcaggctg
     4021 atgaaaaggg gatgatttcc tattatctag tagatcctga tcaagattca tctaaatatc
     4081 aaaagcttgg cgagattgca gaatctaaat ttaaaaattt aggaaatgga aaagagggta
     4141 gtctaaaaaa agatacaact ggggtagaac atcatcatca agaaaatgaa gagtctatta
     4201 aagaaaaatc tagttttact attgatagaa atatttcaac aattagagac tttgaaaata
     4261 aagacctaaa gaaactcatt aaaaagaaat ataaggaaga agacgacttt gtaactggtg
     4321 gaaaaagaac agtagaactt gattataaat atgacgataa aggaaatata actgcctatg
     4381 aagatgaaag tgctctagaa tatgaaactg aaaaatttga cgaaataaaa tcaaaaattt
     4441 atggtgttct aagcccatct aaagatggac actttgaaat tcttggaaag ataagcaatg
     4501 tttctaaaaa tgccaaggta tattatggaa ataactataa atttatagaa atcaaagcga
     4561 ctaagtatga ctcacattca aaaacgatga catttgattt atacgctaat attaatgata
     4621 ttgtggatgg attagctttt gctggagata tgcggttttt tgttaaagat gatgatcgaa
     4681 taaaagctga aactaaaatt agaatgcctg aaaaaaataa ggaaactaaa gcagaatatc
     4741 cctatgtatc aagttatggg aatgtaatcg aattaggaga aggagatctt tcaaagaaca
     4801 aaccagacaa tttaactaaa atggaatctg gtaaaatcta ttctgattca gaaaaacaac
     4861 aatatctgtt aaaggataac atcattctaa gaaaaggcta tgcactaaaa gtgactacct
     4921 ataatcctgg aaaaacggat atgttagaag ggaatggagt ctatagcaag gaagatatag
     4981 caaaaataca aaaggccaat cctaatctaa gagtcctttc agaaacaaca atttatgctg
     5041 atagtagaaa tgttgaagat ggaagaagta cccaagctgt attaatgtcg gctttggacg
     5101 gctttaacat tataaggtat caagtgttta catttaaaat gaacgataaa ggggaagcta
     5161 tcgataaaga cggaaatctt gtgacagatt cttctaaact tgtattattt ggtaaggatg
     5221 ataaagaata cactggagag gataagtcca atgtagaagc tataaaagaa gatggctcca
     5281 tgttatttat tgataccaaa ccagtaaacc tttcaatgga taagaactac tttaatccat
     5341 ctaaatctaa taaaatttat gtacgaaatc cagaatttta tttaagaggt aagatttctg
     5401 ataagggtgg ttttaactgg gaattgagag ttaatgaatc ggttgtagat aattatttaa
     5461 tctacggaga tttacacatt gataacacta gagattttaa tattaagttg aatgttaaag
     5521 acggtgacat catggactgg ggaatgaaag actataaagc aaacggattt ccagataagg
     5581 taacagatat ggatggaaat gtttatcttc aaactggcta tagcgatttg aatgctaaag
     5641 cagttggagt ccactatcag tttttatatg ataatgttaa acccgaagta aacattgatc
     5701 ctaagggaaa tactagtatc gaatatgctg atggaaaatc tgtagtcttt aacatcaatg
     5761 ataaaagaaa taatggattc gatggtgaga ttcaagaaca acatatttat gtaaatggaa
     5821 aagaatatac atcatttgat gatattaaac aaataacaga caagacacta aacattaaga
     5881 ttgttgtaaa agattttgca agaaatacaa ccgtaaaaga attcatttta aataaagata
     5941 cgggagaggt aagtgaatta aaacctcata gggtaactgt gaccattcaa aatggaaaag
     6001 aaatgagttc aacgatagtg tcggaagaag attttatttt acctgtttat aagggtgaat
     6061 tagaaaaagg ataccaattt gatggttggg aaatttctgg tttcgaaggt aaaaaagacg
     6121 ctggctatgt tattaatcta tcaaaagata cctttataaa acctgtattc aagaaaatag
     6181 aggagaaaaa ggaggaagaa aataaaccta cttttgatgt atcgaaaaag aaagataacc
     6241 cacaagtaaa ccatagtcaa ttaaatgaaa gtcacagaaa agaggattta caaagagaag
     6301 atcattcaca aaaatctgat tcaactaagg atgttacagc tacagttctt gataaaaaca
     6361 atatcagtag taaatcaact actaacaatc ctaataagtt gccaaaaact ggaacagcaa
     6421 gcggagccca gacactatta gctgccggaa taatgtttat agtaggaatt tttcttggat
     6481 tgaagaaaaa aaatcaagat taagataaaa gctatagaaa aaaatggttt atgtactgag
     6541 attagatagt gaggtgatga catagttttg tgaaaacagc catttataac tcaattattt
     6601 agtttacttc actattgata ctatttggag ttattaatgg acttagttta gataactaat
     6661 gaattgattg aaagggttag tattgacaat attggtcata ttgattagaa aatagagtct
     6721 atcaaaattt aaaggctaat agaggtgatg agacaatttc ggctctttgt caactgtagt
     6781 gggttgaagt cagctaagct cgagaaagga caaattttgt cctttctttt tt
//
LOCUS       DAVKMU010000076         6687 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKMU010000076 DAVKMU010000000
VERSION     DAVKMU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.076"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..561
                     /locus_tag="VW343_001940"
                     /pseudo
     CDS             1..561
                     /locus_tag="VW343_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            489..941
                     /locus_tag="VW343_001941"
     CDS             489..941
                     /locus_tag="VW343_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756445.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1760..2119
                     /locus_tag="VW343_001942"
     CDS             1760..2119
                     /locus_tag="VW343_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756446.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            2129..2494
                     /locus_tag="VW343_001943"
     CDS             2129..2494
                     /locus_tag="VW343_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9756447.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSRLISQEQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSH"
     gene            2481..4331
                     /locus_tag="VW343_001944"
     CDS             2481..4331
                     /locus_tag="VW343_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9756448.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSYEEMVEMYNVNFTNNDKLYEYRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIMELTGGRFRFIVATHTDKDHIHNHILINAIDCNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRFSYRDYQKYRATSHKFELKQRLYFLMQQSKSFDDFLEKAEQLH
                     VHIDFSQKHSRFMMTDRAMTKPIRGRQLSKRDLYDEDFFRMHFTKQEIASRLEFLLNC
                     VNSLEGLLTKSKELNLTIDLKQKNVIFILEENGKQFSLSHKKISDEKLYDVNFFQDYF
                     KNKEVGVSEGIENLQAQYRAFQEERDKEKVSTEEIEEAFETFKEKRDAVHEFEVKLTE
                     HQIEKLVDEGIYIKVSFGINQSGLIFIPNYQLDIMEEENQKKYKVYIRETTSYFVYNK
                     EHSDKNQYIKGRTLIRQLTNDSRVIPYRRPTVERLQEKISEISLLIELTETDKKYQDI
                     KDNLVSEIAELDIKLTQTNEKIATLNKMAEVLINSKSEGSGSQKLARHEFSKLNMTES
                     TTLEQVNEELLKLQQEFGNVLDEYEKTIRKLGQLFKVFDECINKEIMNEI"
     gene            4587..6019
                     /locus_tag="VW343_001945"
                     /pseudo
     CDS             4587..6019
                     /locus_tag="VW343_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(6257..6391)
                     /locus_tag="VW343_001946"
                     /pseudo
     CDS             complement(6257..6391)
                     /locus_tag="VW343_001946"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         2326 a         1055 c         1308 g         1998 t
ORIGIN      
        1 aagacaattt ttttagtaat tttgataagg ttgttttttc tagttcaaaa gaatatatta
       61 gttttcttat gttagcagag gcaatcataa aaaatggaat agaaatagtt gtagtaactg
      121 atcataatac taccaaaggt attaaaaagt tacaaatggc agtctcaatc ataatgaaaa
      181 attatccgat ttatgatata catcctcata ttttacatgg agtagaaatt agtgcagcag
      241 ataaattgca tattgtatgt atatatgatt atgaacaaga atcatgggtt aatcaatggt
      301 taagtgaaaa tattataagt gagaaagatg gaagttatca acattcactg actataatga
      361 aggatttcaa taatcaaaaa atagttaact atattgctca tttcaatagt tatgacattt
      421 tgaaaaaagg ttctcactta tcaggtgcat ataaacgaaa aattttttct aaagaaaata
      481 cacgattttt ggagtttaat attaactcga aagaatcttc gcaacaactt gatattctct
      541 ataaagaagt tggtgtatta agtttgggac aaaaagttgt agccatgctt gattttttat
      601 tagcatatag tgactattct aaagacttca gaccattgat tattgatcag cctgaagaca
      661 atctagacaa tcgttatatt tacaggcatt tagttcagca gtttagagat gtgaaagctc
      721 aacgtcaaat tattttagca acacataatg ctacaattgt aacaaattct atgacagatc
      781 aagttgttat tatggagtca gatggagtta acggatggat tgaatcacag ggatatgtta
      841 gtgaaaaata tataaaaaat catatcatca atcaattaga gggaggaaaa gattccttca
      901 agcataaaat gtctatatat gagacggctt tatcagagta gagtcagaaa aagtaggtta
      961 gaaatttagc ctactttttt ctttgttcga caggcatagt gtacatctga ggtccaagtc
     1021 ctctgtggat atttgctgca gatgaaacca atagcgactc ctaagcctga atatcgtgag
     1081 gtagggggga taggaaggaa ttagcgaaat caaggttcta caaacagaat cgtgacttga
     1141 agccatatat agcggatgag gaactctaaa atccaaatag gtgtcgtaac ctatatacgt
     1201 aaattacgag agtaaactag gaaagatgta cggcttattc cgtgagcgtt taggacgtag
     1261 tacaacgaat catgggagtc agctgaacac atagtattga agaaatttct gtaatggaaa
     1321 tggagcgaag aagtgaacaa ttaaatgaat acctctctaa ttaaatttgt caattctaat
     1381 tcctggtatg aaaagacagt gacctaaaaa tgtaaacgat gggagctgat cataaatata
     1441 ggacggtaca tgcagtggtg ttagagatta gtccctactt gatttgtgat aacttcccca
     1501 actttcttct gctatacttt tctcaacttt taaaaatcca actaagaatt ttacctggga
     1561 gtttgggggc gtagccacca agttatctta tggttagctg tcaaaactgg aaggttttgg
     1621 ttagctggcg atatgatttt ttgggtattg tgaccacaat gcctgagctc gcaaagaccg
     1681 aacaaggact ttaagaaatt tcccgtatga cacacggggt cggggtttac ccgagaaagg
     1741 atgaaagtga ggaaaagaga tgtctgaaca gtaccgtgac attcgaaaag aggtaaatct
     1801 aaccgcagat gagctaaaac agattgaaaa aatgatggag gttgataatt atcgtcactt
     1861 ttcaccattc gtcagggata aaattttgat gactgatgac aaacagttag ctgccaaaga
     1921 atggttttcc ctttggcagt ctcaaaagtt tgaacagatc agtcgagatg tgcatttggt
     1981 tttgattatc gcaagagaga atcaccaagt gactcaagaa cacgtgtcaa tcttattgac
     2041 ctgcgttcaa gaattgattg cggaagtcaa tcaagtacag tcactcagtc gtgggtttcg
     2101 tgaaaaatac atgagatagg aggctactat ggtttatcgt tatcgtacca atctcaaaaa
     2161 agtatttcta acagattcag aattacacca attgaatgaa cggattgcta agagcaattg
     2221 tcaaaatttt tcagtctatg ccagaaaagt tttgcttaat cccaatatgt catttgtcac
     2281 gattaacacg gatacttatg accagttagt ttttgaattg agacggatcg gaaataacat
     2341 taatcaaatt gcgcgtgcta tcaatcaaag ccgtctgatt tctcaggaac agttacaaga
     2401 attgagtaaa ggagtcggag agttgatcaa ggaagtggac aaagaatttc aagtggaagt
     2461 gaaaagattg aaggagtttc atggtagtca ctaagcattt tgcgacacac ggaaaaaaat
     2521 atcgtaggcg tttaattaag tatatcctca atcctgataa aacggacaat ttgaaattag
     2581 tatctgattt tggtatgagc aattacttag actttcctag ctatgaagaa atggtagaaa
     2641 tgtacaatgt caactttacc aataatgaca agttgtacga atacagaaat gaccgacaag
     2701 aaaaacacca acaaaatatt catgcccatc acctcattca atcattttct cctgaagata
     2761 atctgacacc tgaagaaatt aaccgcattg gttatgagac cattatggaa ttaacaggag
     2821 gccgttttcg tttcatcgta gcgactcata cagacaaaga tcatattcat aatcacatct
     2881 taatcaacgc cattgactgt aattcagata aaaagttgat atggaattat gccttggaac
     2941 gaaaccttcg tatgatttca gaccgcattt ctaaaatggc tggggcgaaa attattgaaa
     3001 agcgtttctc ttaccgtgac tatcaaaaat atagagcgac tagccataag tttgaactaa
     3061 agcaacgtct ttattttttg atgcagcagt caaaatcctt tgatgatttt ttagaaaaag
     3121 cagagcaatt acatgttcat attgatttta gtcagaagca tagtcgattc atgatgacgg
     3181 atcgggcaat gacaaaacca attcgaggac gtcaactcag taaacgagat ttatatgatg
     3241 aagatttttt tagaatgcat tttaccaagc aagagattgc aagtcgttta gaatttttgt
     3301 tgaactgtgt caattcttta gaagggttac taacaaaatc aaaagaattg aatctaacca
     3361 ttgacttaaa acaaaaaaat gtaatcttta tacttgaaga aaatggaaag caattcagtc
     3421 tgagtcataa aaaaataagt gatgagaaat tatatgatgt caattttttt caagattatt
     3481 ttaaaaataa ggaagtaggt gtttcagaag gaatagaaaa tctacaggca cagtaccgtg
     3541 cttttcaaga agaacgagat aaggagaagg tatctactga agaaattgag gaagcctttg
     3601 agacatttaa ggaaaaacga gatgccgttc atgaatttga agtaaaactt acagaacacc
     3661 aaattgagaa gttagttgat gaggggattt atatcaaagt gtcctttggt ataaatcaga
     3721 gtggtcttat tttcattcct aactatcaac tggatattat ggaagaagaa aatcagaaaa
     3781 aatataaagt ctacattcgt gagacaacct catattttgt ctataacaaa gaacattcgg
     3841 ataagaatca gtatatcaaa gggagaacct tgattaggca gctaaccaac gatagtcgag
     3901 taataccata cagaagaccg actgttgaaa gacttcaaga aaagatttct gagattagcc
     3961 tcttgattga gttaactgag acagataaga aataccaaga cattaaagac aacttggttt
     4021 ccgaaatagc agagctagat ataaaactga ctcaaacaaa tgaaaaaatt gccaccttaa
     4081 ataagatggc ggaagtactt atcaattcta agagtgaagg ttcgggtagc caaaaactag
     4141 ctaggcatga attttctaaa ttgaacatga cagaatcaac aactttagaa caagtcaatg
     4201 aagaactttt gaagttacaa caggaatttg gtaatgttct tgatgagtat gagaaaacaa
     4261 taagaaagct tggacaatta tttaaggttt ttgatgagtg tatcaataaa gaaattatga
     4321 atgaaatata aatcagactg aagacaaact caaatttttt tggatatctt ttctgtttat
     4381 ggttgccaat atcggtttaa cttctaaaac aaacattaat aaaaatgcat tcaaagacga
     4441 ttttacctat tttacgatta cgcttcgacc ccaaatcatt aaagttgtct atgctctatc
     4501 attgcatcac tggacttgtc aagagttaag ataattcgga gtccactgaa aaagtcatcg
     4561 tatgatggta tactaaagat gaggtgatgg tatgcttgat aatcgatgtt agtccctatt
     4621 ctactctgtg tgatattgtt gttcccaaaa ctcatttttt acgacagcta atggaactct
     4681 gtgattttag ttttatttat gacgagctgg aaaagaatta tcaacctgac ttcggatgtc
     4741 gttcctactc tctccttatc atgatgttca agtatctctt attaaaagac atctataagt
     4801 tgtccgatgt ggatgtggtg gagcgctcct tttctggtat gaccttcaaa tacttccttg
     4861 gtctagctcc tgttattgaa ccgtcctcat tgactaaatt ccgaaaactt cggaacaaag
     4921 atgaacgatt acttgatttg ctcatcgcta aatctgttca gattgcgata gagctcggtc
     4981 tgattaaaag taacattctc attgtggatg cgactcatac caaagttcat tacaatcaca
     5041 agaaacctca agaagtcctc cgagaacgct caaaagcctt gcgtaaaacg atttatcagt
     5101 attcggaata tatcaaggca gaatttccta gtaagcctca ggaagatact ctagtagctg
     5161 aattaagata tacacaagaa gtaatatcag tccttgagaa gcatgacgaa ttgactggaa
     5221 taccagctat ctcacaaaat tcaattacct taaagaagct gtagaggatg acttagagca
     5281 cttggaatcc tctgtcaaag aagaagcaag aattggacat aagtcagccg atagttcctt
     5341 ttacgggtac aaagagcatt ttgccatgac cgatgaacgg attattactg cctgtgtggt
     5401 gacatcgggt gaaaaaagtg atggtcctgt tttagaagaa ctctatcaca aatctaagga
     5461 taatggcgtg acgattgaag ctattgttgg agatagggcc tactctggga aggataatat
     5521 gcaatttacc aagaaagaac gtgtgcattg agtcgctagg ccatatccaa ctatttcaaa
     5581 aggctttcgt aggaaggaag atgagttttt ctataacaag gacgcaggtc tctttgtttg
     5641 tccagaaggg catatggcac taaagaagga aaagacagga agaaggacgg ctcaggaaac
     5701 ccactatttt gacatcaagc actgtcaaat atgcccttca aaagtgggct gttataaaga
     5761 aggggctaag tctaagactt atttgatacc ccttaagagc gatgaacata ttttccagaa
     5821 gaggtttcag gaagcgccct attttaaaga aaaggccaaa catcgctaca agatagaagc
     5881 caaaaatgcg gaacttgaaa acagacatgg tcttaatgta gctagagcac caggtctttt
     5941 aaacatggag ttacaagccg ccacaaccat atttgtggtt aatatgaaga ggattataac
     6001 tctaataaac caaaaataag gaaaagcaaa gaggaatcta tccaagcagg agatagatcc
     6061 ctcttttttc attttagttg aactgtagag cttacttttt cagtggactc gatgattagt
     6121 gactattttt ttcaaactct attagttatt ggtaccctaa tgccgaatgg agatatttag
     6181 gattgtaata gatttcaata tatttaaaga tttcgttcaa tagttgcgaa attggcatca
     6241 ttgacaagtt ctctttttat gttttgtaaa aggattccat gggtgcatta tcatatggat
     6301 tgccttttcg actcaggcta gacttgcatc ttttctgttt aaggagttct tgaaatctag
     6361 ctccagtata ttgagatcct tgatcggtat ggccgactag ttcttatttg agattttctc
     6421 tatgataagc ttgattgaaa acattcgtta ccaacttatc ttgcattcga tttcccattg
     6481 cctaaccaac aatttttctg ctataaacat atatataatg ataaataaag agtacctgct
     6541 tttgtcggaa tataagttaa atcccccagc cagatttcat ttttaccttt tgtttgaaaa
     6601 cactgattaa tcaaattggg tcgtgttaat gaggaatgcc gacggctata gtacttgtat
     6661 ttatagcgga ttcctttagc atagaga
//
LOCUS       DAVKMU010000077         6655 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKMU010000077 DAVKMU010000000
VERSION     DAVKMU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.077"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(73..165)
                     /locus_tag="VW343_001947"
                     /pseudo
     CDS             complement(73..165)
                     /locus_tag="VW343_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(201..484)
                     /locus_tag="VW343_001948"
                     /pseudo
     CDS             complement(201..484)
                     /locus_tag="VW343_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(474..614)
                     /locus_tag="VW343_001949"
     CDS             complement(474..614)
                     /locus_tag="VW343_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9756449.1"
                     /translation="MVSCHSSLRSYIAEPILKQAGFTVQNLDGAYSLYKMVNPEGVEY
                     GN"
     gene            706..1470
                     /locus_tag="VW343_001950"
     CDS             706..1470
                     /locus_tag="VW343_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9756450.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            2035..3414
                     /locus_tag="VW343_001951"
     CDS             2035..3414
                     /locus_tag="VW343_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9756451.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            3428..4093
                     /locus_tag="VW343_001952"
     CDS             3428..4093
                     /locus_tag="VW343_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9756452.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            4228..4302
                     /locus_tag="VW343_001953"
                     /pseudo
     CDS             4228..4302
                     /locus_tag="VW343_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4448..4987
                     /locus_tag="VW343_001954"
     CDS             4448..4987
                     /locus_tag="VW343_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9756453.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            5054..5464
                     /locus_tag="VW343_001955"
     CDS             5054..5464
                     /locus_tag="VW343_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9756454.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            5580..>6655
                     /gene="pavB"
                     /locus_tag="VW343_001956"
     CDS             5580..>6655
                     /gene="pavB"
                     /locus_tag="VW343_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9756455.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKA"
BASE COUNT         1813 a         1283 c         1525 g         2034 t
ORIGIN      
        1 aaaacagtgt tttgagctga cttcgtcagt tccatccaca accttaaaac agtgttttga
       61 gctgacttcg tcagttccat ccacaacctt aaaacagtgt tttgagcagc ctgcggctaa
      121 cttcctagtt tgcactttaa ttttcattga gtattacttc atcttctcat gaaattgagt
      181 ttttgcccag ctcctcttga ttattctact tgaccgggcc aagcattcat accaccttct
      241 acgttggtaa cggtgaggcc ttgggagctg agaaattggc aggtagatgc agagcggact
      301 cctccgtgac aaatgacata gtattcatgg tcaggtttga gttctttgta gccttgctct
      361 aaggtattta tggaagattt ttgggtcctg cttggaattc atgtgcttca cggacatcga
      421 taagttccag attttcattt tgatattttc tataaaagtc agccatgctg atactagttt
      481 ccatattcta ctccttctgg gttaaccatt ttgtatagtg aataagcgcc gtcaaggttt
      541 tggacggtaa atcctgcttg cttgaggata ggctctgcga tatagctgcg caaactactg
      601 tggcaactga cgatgtaggc ttgtttttct cctcagttca tcagcttcct ccttgacact
      661 cggtcagatt ttgatacaat agtacaaaat tagaggaggt gggctatgat tcagaaacat
      721 gcgattccca ttttagagtt tgatgataat cctcaggcgg ttatcatgcc caatcacgag
      781 gggttggatt tgcagttgcc aaagaagtgt gtctatgctt ttttaggtga ggagattgac
      841 cgctatgcga gggaagtagg ggcgaactgt gttggcgaat tcgtttctgc taccaagacc
      901 tatccagtct atgtcatcaa ctacaagggt gaggaggtct gtctggctca ggctcctgtt
      961 ggctccgctc cagcagccca gtttatggat tggttgattg gctatggtgt ggagcagatt
     1021 atctctactg ggacctgtgg tgtcctagct gatatagagg aaaatgcctt tctagtccct
     1081 gttcgcgctc tgcgagatga aggagccagt taccactatg tggcaccttc tcgttatatg
     1141 gaaatgcagc cagaggctat tgctgccatt gagcgagttt tggatgatag agggattcct
     1201 tatgaagaag tcatgacctg gtccacagat ggtttttacc gagaaacggc tgaaaaggtg
     1261 gcttatcgta aggaagaagg ctgtgctgtt gtggagatgg agtgttctgc tcttgcggca
     1321 gtagctcaat tgcgtggagt tctctggggt gaattgttgt tcacagcaga ttctctagcg
     1381 gacttggacc agtacgacag tcgtgactgg ggctcggaag cttttaataa ggcactagaa
     1441 ctgagtttag caagtgttca ccacctttag ttgtactggc aaaggatttg ttttatcata
     1501 aaatgtctag ctcatacttt tcaaaaatat gtttaaacga ggtcaccttc ctcttgtcct
     1561 aggcatgttg aggttgggaa aaatctttaa aatcagaaaa acgtatcata tcaggtgatg
     1621 aaaattttga tactatgcgt tttatgttgg taagatttag agtgagatga aatgatactc
     1681 ttcgaaaatc tcttcaaacc aggtcagctt caccttgccg tatagatgtt actgacttcg
     1741 tcagttctat ccacaacctc aaaacagtgt tttgagcaac ctgtgactag ctttctaagc
     1801 gatgccttgg ttctcattgc ctataatcaa aaagagaaat tttctcctga aaagcatata
     1861 gagtagctgg cgttaaaagc tcctgtcttg cttttttgac ctatagtcac atctatcaag
     1921 tattgttctt gcctaagcta tcaataaaaa ggtggcattt tttaggcttg gtgttagtag
     1981 attttgcctt atcctatcta agtcatttcg aactttttat ggtacaatgg aaacatgtta
     2041 ttcaaattat ctaaggaaaa aatagagcta ggcttatctc gtttatcgcc agcccgtcgt
     2101 atttttttga gttttgcctt ggtcatttta ctaggctctc ttcttttgag cttgcccttt
     2161 gtccaagttg aaagctcacg agcgacttat tttgatcatc ttttcactgc tgtctctgca
     2221 gtctgtgtga caggtctctc aacccttcca gtagctcaca cctataatat ctggggccaa
     2281 ataatctgtt tgctcttgat tcagatcggt ggtctagggc tcatgacctt tattggggtt
     2341 ttctatatcc agagcaagca aaagcttagt cttcgtagcc gtgcaactat tcaggatagt
     2401 tttagttatg gagaaactcg atctttgaga aagtttgtct attctatttt tctcacgacc
     2461 tttttggttg agagcttggg agctattttg cttagttttc gccttattcc tcaacttggc
     2521 tggggacgtg gtctttttag ttccattttt ctagcgatct cagccttctg taatgccggt
     2581 tttgataatt tagggagcac cagtttattt gcttttcaga ccgatttact ggtcaatctg
     2641 gtgattgcag gcttgattat tacaggcggc cttggtttta tggtctggtt tgatttggct
     2701 ggtcatgtag gaagaaagaa aaaaggacgt ctgcactttc atacgaagct tgtactatta
     2761 ttgactatag gtttgttgtt atttggaaca gcaactactc tctttcttga gtggaacaat
     2821 gctggaacga ttggcaatct ccctgttgcc gataaggttt tagttagctt ttttcaaaca
     2881 gtgacgatgc gaacagctgg cttttctacg atagattata ctcaggctca tcctgtgact
     2941 cttttgattt atatcttaca gatgtttcta ggtggggcac ctggaggaac agctggggga
     3001 ctcaagatta cgacattttt tgtcctcttg gtctttgcac gaagtgagct tctaggcttg
     3061 cctcatgcca atgttgcgag acgaacgatc gcgccgcgaa cggttcaaaa atcctttagt
     3121 gtctttatta tctttttgat gagcttcttg ataggattga ttctgctagg gataacagcc
     3181 aaaggcaatc ctccctttat ccacctcgta tttgaaacca tttcagctct tagtacagtt
     3241 ggtgtaacgg caaatctgac tcctgacctt gggaaattgg ctctcagtgt tatcatgcca
     3301 cttatgttta tgggacgaat tggtcccttg accttgtttg ttagcttggc agattaccat
     3361 ccagaaaaga aagatatgat tcactatatg aaagcagata ttagtattgg ttaagaaagg
     3421 aaagagcatg tcagatcgta cgattggaat tttgggcttg ggaatttttg ggagcagtgt
     3481 cctagctgcc ctagccaagc aggatatgaa tattatcgct attgatgacc acgcagagcg
     3541 catcaatcag tttgagccag ttttggcgcg tggagtgatt ggtgacatca cagatgaaga
     3601 attattgaga tcagcaggga ttgatacctg cgataccgtt gtagtcgcga caggtgaaaa
     3661 tctggagtcg agtgtgcttg cggttatgca ctgtaagagt ttgggggtac cgactgttat
     3721 tgctaaggtc aaaagtcaga ccgctaagaa agtgctagaa aagattggag ctgactcggt
     3781 tatctcgcca gagtatgaaa tggggcagtc tctagcacag accattcttt tccataatag
     3841 tgttgatgtc tttcagttgg ataaaaatgt gtctatcgtg gagatgaaaa ttcctcagtc
     3901 ttgggcaggt caaagtctga gtaaattaga cctccgtggc aaatacaatc tgaatatttt
     3961 gggtttccga gagcaggaaa attccccatt ggatgttgaa tttggaccag atgacctctt
     4021 gaaagcagat acctatattt tggcagtcat caacaaccag tatttggata ccctagtagc
     4081 attgaattcg taaagaggga tgacccctct tttttgatgc ctaagatggc aaatagagac
     4141 agaagcccct tgtcttctag taaaagttct tcaaaggctg gactttatgg taaaatagaa
     4201 agaagtgaca agagagagta atactcaatg aaaatcaaag atcaaactag gaaactagct
     4261 acgggctgct caaaacactg ttttgaggtt gcagatagaa ctgacgaagt cagctcaaaa
     4321 cactgttttg aggttgcaga tagaactgac gaagtcagta acatctatac ggcaaggcga
     4381 cgttgacgcg gtttgaagag attttcgaag agtataagaa aaaatcagtc ccctaaagga
     4441 gtagattatg aagttattgt ctatcgcaat ttctagctat aatgcagcag cctatcttca
     4501 ttactgtgtg gagtcgctag tgattggtgg tgagcaagtt gggattttga ttatcaatga
     4561 cgggtctcag gatcagactc aggaaatcgc tgagtgttta gctagcaagt atcctaatat
     4621 cgttagagcc atctatcagg aaaataaatg ccatggcggt gcggtcaatc gtggcttggc
     4681 agaggcttct gggcgctatt ttaaagtagt tgacagtgat gactgggtgg atcctcgtgc
     4741 ctacttgaaa attcttgaaa ccttgcagga acttgagagc aaaggtcaag aggtggatgt
     4801 ctttgtgacc aattttgtct atgaaaagga agggcagtct cgtaagaaga gtatgagtta
     4861 cgattcagtc ttgcctgttc ggcagatttt tggctgggac caggtcggaa atttctccaa
     4921 aggccagtat accatgatgc actcgctgat ttatcggaca gatttgttgc gtgctagcca
     4981 gttctaactg cctgaacata ctttttatgt cgataatctc tttgtcttta cgccccttca
     5041 gcaggtcaag accatgtact atctgcctgt cgatttctat cgttatttga ttgggcgtga
     5101 ggaccagtct gtcaatgagc aagtgatgat taagtgcatt gaccagcaac tcaaggtcaa
     5161 tcgactcttg atagaccaac ttgatttgtc ccaagtgagt catcccaaaa tgcgagaata
     5221 tctgctgaat catattgaac tcacgacggt gatttccagt accctgctca accgatctgg
     5281 aacagcggag catctggcaa aaaaacgcca attgtggacc tatattcagc agaaaaatcc
     5341 agaagtcttt caggctattc gtaagaccat gttgagccgt ttgaccaaac attctgtctt
     5401 gccagatcgc aaactgtcca atgtcgtcta tcaaatcacc aaatctgttt atggatttaa
     5461 ttaatataag tgttttataa gagggattta agaaaaattt taactttttc ttagtccttt
     5521 ttaatttcag gagattatac tagagtcatc aaataaagaa agactctaag gagaatccta
     5581 tgaaattcaa tccaaatcaa agatatactc gttggtctat tcgccgtctc agtgtcggtg
     5641 ttgcctcagt tgttgtggct agtggcttct ttgtcctagt tggtcagcca agttctgtac
     5701 gtgccgatgt ggtcaatccg accccaggtc aagtcttacc tgaagagaca tcgggaacga
     5761 aagagggtga cttatcagaa aaaccaggag acaccgttct cactcaagcg aaacctgagg
     5821 gcgttactgg aaatacgaat tcacttccga cacctacaga aagaactgaa gtgagcgagg
     5881 aaacaaactc ttctagtctg gatacacttt ttgaaaaaga tgaagaagct caaaaaaatc
     5941 cagagctaac agatgtctta aaagaaactg tagatacagc tgatgtggat gggacacaag
     6001 caagtccagc agaaactact cctgaacaag taaaaggtgg agtgaaagaa aatacaaaag
     6061 acagcatcga tgttcctgct gcttatcttg aaaaagctga agggaaaggt cctttcactg
     6121 ccggtgtaaa ccaagtaatt ccttatgaac tattcgctgg tgatggtatg ttaactcgtc
     6181 tattactaaa agcttcggat aatgctcctt ggtctgacaa tggtactgct aaaaatcctg
     6241 ctttacctcc tcttgaagga ttaacaaaag ggaaatactt ctatgaagta gacttaaatg
     6301 gcaatactgt tggtaaacaa ggtcaagctt taattgatca acttcgcgct aatggtactc
     6361 aaacttataa agctactgtt aaagtttacg gaaataaaga cggtaaagct gacttgacta
     6421 atctagttgc tactaaaaat gtagacatca acatcaatgg attagttgct aaagaaacag
     6481 ttcaaaaagc cgttgcagac aacgttaaag acagtatcga tgttccagca gcctacctag
     6541 aaaaagccaa gggtgaaggt ccattcacag caggtgtcaa ccatgtgatt ccatacgaac
     6601 tcttcgcagg tgatggtatg ttgactcgtc tcttgctcaa ggcatctgac aaggc
//
LOCUS       DAVKMU010000078         6472 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKMU010000078 DAVKMU010000000
VERSION     DAVKMU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.078"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..776)
                     /locus_tag="VW343_001957"
     CDS             complement(<1..776)
                     /locus_tag="VW343_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEU9756456.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKS"
     gene            1084..2064
                     /locus_tag="VW343_001958"
     CDS             1084..2064
                     /locus_tag="VW343_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9756457.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDDFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKKGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            2869..3096
                     /locus_tag="VW343_001959"
     CDS             2869..3096
                     /locus_tag="VW343_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756458.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            3384..6281
                     /gene="nanA"
                     /locus_tag="VW343_001960"
     CDS             3384..6281
                     /gene="nanA"
                     /locus_tag="VW343_001960"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9756459.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKEIDGKTYQILY
                     REGEKEAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         2101 a         1110 c         1405 g         1856 t
ORIGIN      
        1 gattttcgtt caaactgatt cataatttga acacgaaaac gactcatggc acggctgaga
       61 tgttggataa tatggaaacg atctagaacg attttagcac acggaaaaag ctgtttagcc
      121 aagtcatagt aaggactaaa catatccatc gtaatgattt tcacttgaca acgaacggct
      181 ctatcgtagc gaagaaagtg atttcggatg acagcttgtg ttctgccttc aagaacagtg
      241 ataatattaa gattatcaaa atcttgcgca atgaaactca tctttccctt agtgaaggca
      301 tactcatccc aagacataat ctttggaagc cgagaaaaat catgctcaaa gtgaaagtca
      361 ttgagcttgc gaatgacagt tgaagttgaa atggccagct gatgggcaat atcagtcata
      421 gaaatttttt caattaactt ttgagcaatt ttttggttga tgatacgagg gatttggtga
      481 tttttcttta ccaggggagt ctcagcaacc atcatttttg aacagtgata gcacttgaaa
      541 cggcgttttc taaggagaat tctagaaggc ataccagttg tttcgaggta agggatctta
      601 gacggttttt gaaagtcata tttcttcatt agacttccac aatcagggca agatggagcc
      661 tcataatcca gcttagcgat aatttctttg tgggtatcca tattgatgat atctagaatc
      721 ttgatgtttg ggtctttaat atcgagcagt tttgtgataa aatgtaattg ttccatatga
      781 atctttctaa tgatggtttt gtcgcttttc attataggtc atatgggact ttttttctac
      841 aataaaatag gctccataat atctatagtg gatttaccca ctacaaatat tatagagccg
      901 caaaaaacta gtataaacag aagagagagc gaaatgctct tttttcgttt ctaaaactac
      961 tttcagccca tcatcctaaa agtaaagaat ctaaattcac tttctattta cccttctttc
     1021 ttgcattgat tacatagata tgctacagtt gtggtaacga ttacaaaata aaaggagcat
     1081 gctatgaaaa atccagcttt gctagaagaa attaagacct atagaggaag ggatgaggtt
     1141 ccggaagact ttgatgattt ctgggatggg gaagtgaaaa atgtttccac gcttccatcc
     1201 taccacttgg aggaaagaga tttccacatt cctcaagtca agtgctatga gttaacattt
     1261 gaaggaagca agaaaggaaa ggtctatgca cgcattgttc ttccaaagag tgaggagaag
     1321 gttccactaa tcttccattt tcatggttat atgggacgtg gctgggactg ggccgacatg
     1381 ctgggcttca ccgtagctgg ttacggtgtt gtttccatgg atgtgcgggg ccagtcaggt
     1441 tactcacaag acggcttgcg ttctccttta ggaaataccg tgaaggggca tattatccgt
     1501 ggtgctgtgg aaggtcggga ccacctcttt tataaggatg tttatctgga tatttaccag
     1561 ttggtcgaaa ttgttgctag tctgtctcag gttgatgaga agcgtctttc tagctatggt
     1621 gcctcacaag gaggggctct agctctagtt gcagcagcgc tcaatcctcg aattcagaaa
     1681 acagttgcca tttatccctt cttgtcagac ttcagacggg tgattgagat tggtaatact
     1741 agcgaggctt acgacgaact tttccgttat ttcaagtttc acgacccctt ccatgaaaca
     1801 gaggaggaaa tcatggcgac ccttgcctat atcgatgtca aaaatcttgc ccatcgtatc
     1861 caaggtgagg ttaagatgat tacgggcttg gacgacgatg tttgctatcc cattacccag
     1921 tttgcgattt ataatcgtct gacctgcgat aaaacctatc gcatcatgcc tgagtatgct
     1981 cacgaagcca tgaatgtatt tgtcaatgac caagtctaca actggctctg tggaagtgag
     2041 attcctttta aatatctaaa ataaggagtc gactctaagc acaaaatctt aaaaattaca
     2101 aacacgcata gtatcagggg attaagaaaa cttgatacta tgcgttttat catggaaata
     2161 tagtaaaatg aaacaagaac aggacaaatc gatcaggaga gtcaaatcga tttctaacaa
     2221 tgttttagaa gtagatgtgt actattctag tttcaatcta ttatatttat agaatttttt
     2281 gttgctagat ttgtcaaatt gcttaaaata atttttttca gaaagcaaaa gccgatacct
     2341 atcgagtagg gtagttcttg ctatcgttag gcttgtctgt aggtgttaat acttttcaaa
     2401 aatctcttca aaccacgtca gcttcgcctt gccgtatata tgttactgac ttcgtcagtt
     2461 ctatctgcca cctcaaaacg gtgttttgag ctgactttgt cagtcttatc tacaacctca
     2521 aaacagtgtt ttgagcatca tgcggctagc ttcttagttt gctctttgat tttcattgag
     2581 tataaaaaca gatgagtttc tgttttcttt ttatggacta taaatgttca gctgaaacta
     2641 ctttcaagga cattattata taaaagaatt ttttgaaact aaaatctact atattacact
     2701 atattgaaag cgttttaaaa atgaggtata ataaatttac taacacttat aaaaagtgat
     2761 agaatctatc tttatgtata tttaaagata gattgctgta aaaatagtag tagctatgcg
     2821 aaataacaga tagagagaag ggattgaagc ttagaaaagg ggaataatat gatatttaag
     2881 gcattcaaga caaaaaagca gagaaaaaga caagttgaac tacttttgac agtttttttc
     2941 gacagttttc tgattgattt atttcttcac ttatttggga ttgtcccctt taagctggat
     3001 aagattctga ttgtgagctt gattatattt cccattattt ctacaagtat ttatgcttat
     3061 gaaaagctat ttgaaaaagt gttcgataag gattgagcag gaagtatggt ataaatagca
     3121 taagctgatg tccatcattt gcttataaag agatatttta gtttaattgc agcggtgtcc
     3181 tggtagataa actagattgg caggagtctg attggagaaa ggagagggga aaattggcac
     3241 caatttgaga tagtttgttt agttcatttt tgtcatttaa atgaactgta gtaaaagaaa
     3301 gttaataaaa gacaaactaa gtgcattttc tggagtaaat gtcttatttc agaaatcggg
     3361 atatagatat agagaggatc agtatgaatc ggagtgttca agaacgtaag tgtcgttata
     3421 gcattaggaa actatcggta ggagcggttt ctatgattgt aggagcagtg gtatttggaa
     3481 cgtctcctgt tttagctcaa gaaggggcaa gtgagcaacc tctggcaaat gaaactcaac
     3541 tttcggggga gagctcaacc ctaactgata cagaaaagag ccagccttct tcagagactg
     3601 aactttctgg caataagcaa gaacaagaaa ggaaagataa gcaagaagaa aaaattccaa
     3661 gagattacta tgcacgagat ttggaaaatg tcgaaacagt gatagaaaaa gaagatgttg
     3721 aaaccaatgc ttcaaatggt cagagagttg atttatcaag tgaactagat aaactaaaga
     3781 aacttgaaaa cgcaacagtt cacatggagt ttaagccaga tgccaaggcc ccagcattct
     3841 ataatctctt ttctgtgtca agtgctacta aaaaagatga gtacttcact atggcagttt
     3901 acaataatac tgctactcta gaggggcgtg gttcggatgg gaaacagttt tacaataatt
     3961 acaacgatgc acccttaaaa gttaaaccag gtcagtggaa ttctgtgact ttcacagttg
     4021 aaaaaccgac agcagaattg ccaaatggtc gagttcgcct gtatgttaat ggtgttttat
     4081 ctcgaacaag tctgaaatct ggcaatttca ttaaagatat gccagatgta acgcatgtgc
     4141 aaatcggagc aaccaagcgt gccaacaata cggtttgggg gtcaaatcta cagattcgga
     4201 atctcactgt gtataatcgt gctttaacac cagaagaggt acaaaaacgt agtcaacttt
     4261 ttaaacgctc agatttagaa aaaaaactac ctgaaggagc ggctttaaca gagaaaacgg
     4321 acatattcga aagcgggcgt aacggtaaac caaataaaga tggaatcaag agttatcgta
     4381 ttccagcact tctcaagaca gataaaggaa ctttgatcgc aggtgcagat gaacgccgtc
     4441 tccattcgag tgactggggt gatatcggta tggtcatcag acgtagtgaa gataatggta
     4501 aaacttgggg tgaccgagta accattacca acttacgtga caatccaaaa gcttctgacc
     4561 catcgatcgg ttcaccagtg aatatcgata tggtgttggt tcaagatcct gaaaccaaac
     4621 gaatcttttc tatctatgac atgttcccag aagggaaggg aatctttgga atgtcttcac
     4681 aaaaagaaga agcctacaaa gaaatcgatg gaaaaaccta tcaaatcctc tatcgtgagg
     4741 gagaaaagga agcttatacc attcgagaaa atggtactgt ctatacacca gatggtaagg
     4801 cgacagacta tcgcgttgtt gtagatcctg ttaaaccagc ctatagcgac aagggtgatc
     4861 tatacaaggg tgaccaatta ctaggaaata tctacttcac aacaaacaaa acttctccat
     4921 ttagaattgc caaggatagc tatctatgga tgtcctacag tgatgacgac gggaagacat
     4981 ggtcagcgcc tcaagatatt actccgatgg tcaaagccga ttggatgaaa ttcttgggtg
     5041 taggtcctgg aacaggaatt gtacttcgga atgggcctca caagggacgg attttgatac
     5101 cggtttatac gactaataat gtatctcact taaatggctc gcaatcttct cgtgtcatct
     5161 attcagatga tcatggaaaa acttggcatg ctggagaagc ggtcaacgat aaccgtcagg
     5221 tagacggtca aaagatccac tcttctacga tgaacaataa acgtgcgcaa aatacagaat
     5281 caacggtggt acaactaaac aatggagatg ttaaactctt tatgcgtggt ttgactggag
     5341 atcttcaggt tgctacaagt aaagacggag gagtgacttg ggagaaggat atcaaacgtt
     5401 atccacaggt taaagatgtc tatgttcaaa tgtctgctat ccatacgatg cacgaaggaa
     5461 aagaatacat catcctcagt aatgcaggtg gaccgaaacg tgaaaatggg atggtccact
     5521 tggcacgtgt cgaagaaaat ggtgagttga cttggctcaa acacaatcca attcaaaaag
     5581 gagagtttgc ctataattcg ctccaagaat taggaaatgg ggagtatggc atcttgtatg
     5641 aacatactga aaaaggacaa aatgcctata ccctatcatt tagaaaattt aattgggaat
     5701 ttttgagcaa aaatctgatt tctcctaccg aagcgaaagt gaagcgaact agagagatgg
     5761 gcaaaggaga gatgggcaaa ggagttattg gcttggagtt cgactcagaa gtattggtca
     5821 acaaggctcc aacccttcaa ttggcaaatg gtaaaacagc gactttccta acccagtatg
     5881 atagcaagac cttgttgttt gcagtagata aggaagatat cggacaggaa attattggta
     5941 tagctaaagg aagcatcgaa agtatgcata atcttcctgt aaatctagca ggtgccagag
     6001 ttcctggcgg agtaaatggt agcaaagcag cggtgcatga agttccagaa tttacagggg
     6061 gagttaatgg tacagagcca gctgttcatg aaatcgcaga gtataaggga tctgattcgc
     6121 ttgtaactct tactacaaaa gaagattata cttacaaagc tcctcttgct cagcaggcac
     6181 ttcctgaaac aggaaacaag gagagtgacc tcctagcttc actaggacta acagctttct
     6241 tccttggtct gtttacgcta gggaaaaaga gagaacaata agagaagaat tctaaacatt
     6301 tgattttgta aaaatagaag gagatagcag gtttttaagc ctgctatctt tttttgatga
     6361 cattcaggct gatacgaaat cataagaggt ctgaaactac tttcagagta gtctgttcta
     6421 taaaatatag tagattgaaa taagatgtga acaactctat caggaaagtc aa
//
LOCUS       DAVKMU010000079         6461 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKMU010000079 DAVKMU010000000
VERSION     DAVKMU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.079"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(87..1511)
                     /locus_tag="VW343_001961"
     CDS             complement(87..1511)
                     /locus_tag="VW343_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9756460.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVAEKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2056..4335)
                     /gene="pulA"
                     /locus_tag="VW343_001962"
     CDS             complement(2056..4335)
                     /gene="pulA"
                     /locus_tag="VW343_001962"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9756461.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPYGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4447..6405)
                     /gene="ligA"
                     /locus_tag="VW343_001963"
     CDS             complement(4447..6405)
                     /gene="ligA"
                     /locus_tag="VW343_001963"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9756462.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERGNLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
BASE COUNT         1871 a         1446 c         1189 g         1955 t
ORIGIN      
        1 accaaaaagt tcgtattttt tctataatta ttattatttt atagcaaaaa aataaaacca
       61 ggaaaacaat ttcctgattt tacacgttat ttcacatcaa aaacaatgga tttgacattt
      121 gtcatcgctt caatgctata tttaattcct tgcactccag ctccagaacc tttgacacca
      181 aggaatggga aattatctgg accacgctgg gttttattat taatgtggac tgtacctact
      241 tcaagttttt cagcaatttc aaaggctttt ttgaaatcat ttgtaaagac tgatgattga
      301 aggccgaatt cagattcgtt ggcaaaggca atagcttcct ctacactagc cacacgaatg
      361 attggtaaaa caggaccaaa tggctcttcc catgccactt tcatatcttt tgtaacttgg
      421 tcaaaaagca ctggccagag gagattgccc tcacgtttga ttggtgtaag agcctgagcc
      481 tctttttctt gtgcatcctc aatcaagccc caaatgaagt cggctgaagc attgtcaata
      541 acaggtgtaa tatcagcatt gtcaaatgga tcaccaactg ttaatttaga aacttcttcc
      601 tgaagcaaag tagctaattt ctctgctaca ctttcgagaa caatgacacg tttaatggcc
      661 gtgcaacgtt gtcctgagta gctaaaggct cccgcaacaa tttgcttggc agcatgttcc
      721 aaatctgcat cttctagtac aagagctgca tctttcccac caagttccaa catgatagga
      781 cgcataccag ctaaacgacc aatacgttct ccaataggag ttgaacctgt aaagttgatg
      841 aagttgactt ctttgtgctc aatgatataa tccccaattt ctgaaccacg acctgtaatg
      901 gtgttgaaaa cacctgccgg aatccctgct tcttcaaatg ctttagccaa caagagtcca
      961 gaaatggaac cttgtgttgg tggcttaaac atgaccacat tccctgcaat caaggcaggt
     1021 gcaattttag aagcagataa attaactgga taattaaagg gagcaatcgc tagcacgata
     1081 ccaactggtt cacgacggac aacagccagt ttgtttttac ttgctgcctc aaaaccacca
     1141 ccttccattg cttgtccagt gatacggaga ccttcctcag cagcataacg aatcaagtct
     1201 gctgtacgca ctacttctcc aattgctgct ttaatccctt ttgctacttc tttggcaagg
     1261 atagtaccaa tttcttcctt atcgcgttct aaaatagctg ctgttttatg taaataagcc
     1321 gcacgttcaa ctgctgataa agctcgccat gctggcaggg ctgcacgcgc agcttgcata
     1381 gcctcatcag cttcagtctg agtcatggct ggaactgtac ccaattcttc ttgattgatt
     1441 ggtgaataaa tcgtaatttc ttgttcagat gatttccatt ttccatttac taaattctga
     1501 tatcttgtca aattcttgtc ctcctgaaaa tgattaagat atattttacc aaattttcag
     1561 aaaatcacaa ccctttttgc aaaatttttg agattatttt cacaaacttg atttttcaaa
     1621 gtatactcaa taaaaataaa aaaaatccac tacgtcaagg cgaggctaat gtggtttgaa
     1681 gaaattttcg aagagcgtga atgagtatca tctatagtaa aataaaaaaa ctgaacaatt
     1741 tggttgggga cagccaaacc aatttctcac aatgtttcag aaacaagggt gtgctactcc
     1801 aatttcagcc tactataact gtcatagatt gctgaaacaa agtctaggta aaagtcttca
     1861 taataaaaag acctcctatc aagtgttcaa aaactttgat aggaggtctt gttttgtgaa
     1921 aatatttatc aaattttcta tacaagtgag ctgttagcca ggttctttct attctttcaa
     1981 tttcaatgaa tggatttttt actaatactc ataactggga atttgtctgt gtaaaaatag
     2041 cgagatagat ggtatttata aaacactcaa gacagctaga ctaatatcat ttaaaacatt
     2101 atcttctttt gagcgactgt tggttaccaa catagctaaa tttcctgcat tttcaaattg
     2161 atagggttct gatttagcat tcacaaccac caagaggtgt tctttgccgt gaacttcata
     2221 gataaggtag ccgctatgtt caatcgcaga atgcacaaag acatgatggt aaatttcatc
     2281 atagctagag taagaaaagg caccagtttt tgtcttcaat cggatgactt gacggataaa
     2341 ctcaatactg tcttgacgct cattaatcaa gttccagttc acttggttca cactgtcagg
     2401 agcattatag ctattcatcg cacgctctct atcatcatgg gtcaactcac cattttcacc
     2461 agtcgcaacc agtttggtac gaccaaattc ttgaccgatt tccataaagg ccatcccctg
     2521 catgagcaga ttcatggctg tggcagtttc gaccttgcgc atgatttgct ctgaactttg
     2581 gtctggatga agggttgcca ataaatcgtg aagattgtaa ttgtcatggg cttctacata
     2641 gttaagcacc tgatttggat gtgtatagct tcctaattca cgacttccta ggattgcttt
     2701 agctagaatt ggctctgtcg cagcaccact gacaaaacct gacttgatag caccataaac
     2761 ttctccccct ttgacagcat cgcgctgatt gtcattaaag aaaccaatat ttggcatctg
     2821 gtaggcattg tccttcttgg ccttatcata aggggcaaga cctgttccca tatcccatcc
     2881 ttctccatag aggataatgt tggagtcgat ttcatccaag ctttgacgaa tcatctgcat
     2941 ggtcttgaca tcatgaatcc ccatcaagtc aaaacggaag ccgtcaatat tatattcctg
     3001 cacccagtat agaagagaat caatcatata cttgcgaaac atttcgtgtt cactggctgt
     3061 ttcatttcca acacccgttc cattctggaa ggtaccatat ggattcatac gataatagta
     3121 atcagggact gttgtttgga atggtgcatc aacaactgag aaggtatggt tatagactac
     3181 atccataatg actccaatac ccgcatcgtg ataagcttga accatcacct tcaaatcacg
     3241 aatgacctga gctggatcat ctggattagt tgaaaaacta gtttctggcg cgttatagtt
     3301 ttgtggatca taaccccagt tgtaggttac atttccatcc tcatcgtatt ctttatgacg
     3361 gtctgcaatt ggttgcaatt gaacataatt gtagcccagc ttcttgatgt aatcaaaagc
     3421 agttgactgg ccgtattggt taactgttcc agcctgagca gcacccaaga aagttcctcg
     3481 aagatgttca tctacacccg atgtaggtga tttagtcaaa tcacgaatat gcatttcaca
     3541 gataactgcc ttacatggat tttccaagcg ccaagtagcc tccgaaccgt gcttaacctc
     3601 gaagttttca acttgctttt ctacatggct cagaatagct gaacgtttgc catcagggct
     3661 ggtcgcgatt gtataaggat cacgtgtcag tgtttggtga tgagggaatt ggacttgata
     3721 ctgataagcc ttacctacca aatcttcctc aacatccaaa ctccagacac cgattgtatt
     3781 gtccttatga ttataagagt agctattgcc tcttttcatc tcaaaagtct tccaaacggg
     3841 tgcatcatta gcagctgatt cataaacgac aacttgcact tctgtcgctg taggtgacca
     3901 gagggaaaaa tgagcctgat tgtcctctac acggcaaccc aattctcctt ggtaacccca
     3961 atgatggtca aaactagcac tgttaatggc cttatcaaag gcaaaaggat tttgattttt
     4021 atagaaagga ctggcaatag caggattttc agagtaataa atcctatcat cgccttccaa
     4081 aatccagacc tctgttaata ggggatagtg attaaaacgg atagaatatt ctttactagt
     4141 ttgacctgta tgaaccacaa aattcaagct ttctataaca tgtgaacttg ggtgttcaaa
     4201 gctaaataaa gctccaaaat aatcttcttt gtaggttagc aaatcaattc gttgatcctg
     4261 actttttaca aaggagcaag tgtcatattc tccattctta cgatggtaat gaatgcgcat
     4321 agggtagtta tacatttttt atttttcctt tttactttgt ttctatttca ctaataaatt
     4381 tttgtcaatc tcgtctcaat taacagacat agtcatattc tctaaactct gtttttaaac
     4441 gatccattac aaactttcta gccatgcctc atctctgacc tggataccaa gttcttgtgc
     4501 tttttgcagt ttacttccag cgtctgcacc taccacgacg aggtcggtct ttttagaaac
     4561 actacctgtc actttggcac ccagactttc gagtttactt ttagcttctg agcgcttgag
     4621 tcgttccaat tttcctgtca ataccacggt caaacctgac aaggccgcat ccgctactac
     4681 cgtctgtcct ttatagtcca gattgacccc agtttctttc aattctctga gcagaatttc
     4741 agagccttct gtcgcaaaat aagtctgaag acttttggca atcacgccac ctagactttc
     4801 aatactagcc acttcctctg aatctgcctg atacagattt tcaattgaat ggaaatattg
     4861 aagtaaaagc tgactagcct tgcttccgac atgacgaatt cccaaaccaa ataagagctt
     4921 ctcggcagaa ttttcctttg atgcttggat agcctgatac agtttagcag cggacttttc
     4981 cttaaccccc tctaaaagga ggaaatcctc ttcttgcaaa cgataaatat ccgccacatc
     5041 cttgactaaa ttagcagcaa aaagcttctc aacaatagat ggaccaaggc ctgtaatatt
     5101 catagcatca cgagaagcaa agtgaatcaa gccttccatg atttgagcag ggcaacgcgg
     5161 attgatacaa cgtagggcca cttcatcttc aaagtgcaac aagtcagagt tacaacttgg
     5221 acagtttgta gggatatcta gtttttcttc agaaacccgt ttggactcta ccacacgtaa
     5281 aacggcaggg atgatgtcac cagccttata tacaatgacc gtatcgtctt ttcggatatc
     5341 tttttcagca atataatcta cattgtgcag ggtcgcacgg ctaacagtcg taccggcaag
     5401 ttgtactggt gttagattag cagttggagt tacaacaccg gtacggccaa ctgtccagtc
     5461 aactgataag agttgagctt ctttttcttc agcagggaac ttgtaggcta ctgcccactt
     5521 tggagcctta acggtaaaac caagttcttc ttgacttgct aggtcgttga ccttgattac
     5581 cactccatca atatcgtaag gcagatttcc ccgttcctgt cctacttctt ggataaaatt
     5641 ccagatttca tctatgtttt cagccaagat tcgcttagga ttgaccacaa aacctagttg
     5701 ttctaggtac ttcaaaccct tttcttggct atcacgagtt gaagggctgg cttcttgata
     5761 gagaaacgtt gcaagattac gcttggcaac tactgctgta tccaactgac gcagagttcc
     5821 tgctgccgca ttacgaggat tagcaaattc aggctctcca ttttcttggc gcacttggtt
     5881 aacttggtca aaggaagcgc gtggcatgta acattcccca cgaactgtga tatctagttc
     5941 ttctggcaaa gtcaaaggga tgtccttaac acgcttgagg ttttctgtga tattttcacc
     6001 aattgaacca tctccacgtg ttaccccagc aaccaaaatc cccttttcat aagtcagcga
     6061 gatagataag ccatcgattt tcagctcaca aatataggtc ggatgagcca cttccttacg
     6121 aacacgcgca tcaaaagcat ctagctcctc acgtgaaaaa gcatcctgca aactataaag
     6181 aggatactga tgactgtatt tttcaaaacc atctaaaacc ttgccaccaa cacgatgagt
     6241 cggactgtct gctagcactt gctctggata agcagtttct aactcgacca actcacggta
     6301 aaggcggtca tactcactgt ctgaaaccga gggattatcg ctggtatagt actcagtcgc
     6361 atagcgattg agcaaagcga ctaactcatt cattctttta ttcataagac cattttacca
     6421 taaaacaagc cctcctcaca aacgagaagg gcggaaaaaa c
//
LOCUS       DAVKMU010000080         6092 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKMU010000080 DAVKMU010000000
VERSION     DAVKMU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.080"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..644
                     /locus_tag="VW343_001964"
                     /pseudo
     CDS             1..644
                     /locus_tag="VW343_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            755..1681
                     /locus_tag="VW343_001965"
     CDS             755..1681
                     /locus_tag="VW343_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9756463.1"
                     /translation="MDLSQTMPSYAQVVSLYDDTSISVSNNETDKVLAGSLYTDTNEQ
                     GLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQFQTKIVRVINDE
                     DDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQKNKKYTVLS
                     QQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIGVMKAVGYQ
                     NRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFELNLLSVLG
                     TLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1858..2865
                     /gene="gap"
                     /locus_tag="VW343_001966"
     CDS             1858..2865
                     /gene="gap"
                     /locus_tag="VW343_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9756464.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2932..3768)
                     /locus_tag="VW343_001967"
     CDS             complement(2932..3768)
                     /locus_tag="VW343_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9756465.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3894..6089
                     /locus_tag="VW343_001968"
     CDS             3894..6089
                     /locus_tag="VW343_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9756466.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
BASE COUNT         1867 a         1102 c         1311 g         1812 t
ORIGIN      
        1 tagacttaaa aactatctta ctgacaatcc agacgcttat ttgactgaaa tagcttctga
       61 atttggctgt catccaacta ccatccacta tgcactcaaa gctatgggct acactcgaaa
      121 aaaagaacca cacctactat gaacaagacc cagaaaaagt agccttattt cttaagaatt
      181 ttaatagttt aaagcaccta tcacctgttt agattgatga aacaggattt gatacttatt
      241 tttatcgaga atatggtcgc tctttaaaag gtcagttaag aagaagatat cagaggattt
      301 ctttggttgc aggtctaaca aatgaatggt gagttaatcg ctccaatgac ttacgaagag
      361 acgatgacga gcgacttttt tgaagcatgg tttcagaagt ttctcttacc aacattaacc
      421 acaccatcgg ttattattat ggataatgca agattccata gaatgggtaa gttagaactc
      481 ttgtgtgaag agtttgggca taaactttta cctcttcctc cctactcacc tgagtacaat
      541 ctattgagaa aacatgggct catatcaaaa agcacctcaa aaaggtatta ccaagttgca
      601 atacctttta cgaggctttt ttatcctgct cttgtttcaa ttgactatag ttcacggata
      661 cagttgggaa agaagctatt aaatatttgg agagtccttt tgggatgaac atgtcaaata
      721 ttactataga tgggcaagaa aagatgggca agaaatggat ctgagtcaaa cgatgccttc
      781 ttatgctcaa gtagtgagtc tttatgatga tacaagtatc tctgttagta ataatgagac
      841 ggacaaagtg ttggctggct ccctctatac tgatacaaat gaacagggat taacgattcc
      901 aagcagttta ctaaaaaatt ggaatgaaca gacaggaaaa aatttgacag ctaatgatct
      961 tattggcaaa tcagtctcag ccagcattgt agaaagtgct gccgaaacta gtaagattgc
     1021 tcaatttcaa acgaagattg tacgtgtaat caatgatgaa gatgacatgg aggacagcaa
     1081 cagtttcatg ctgtctcatc aaatggaaac gattttgaaa gaggctggat ttacgaaagc
     1141 tgtatcttat tttatcttgg aactcaaaga tccatcacag acaaaagtag taacagaaga
     1201 attacagaaa aataagaagt atactgtgct ttctcaacag agagttcttg atattgtgat
     1261 tacctttatt cgtgttattc agggattatt gattgtgctt tcatcacaag ctattgtggt
     1321 agcagcggtt atgattggta tcattattta catcaatatc atgcaacgtt ccaaggaaat
     1381 aggtgtcatg aaagcagttg gttatcagaa tcgtggtgtc aaaggaattt ttatttacga
     1441 ggctatctgg attgtaggca tcgccttgct gctggcattt ttggttgcac aaggggtggg
     1501 aagtttggcg aatgcgattg taagtcactt ttacccatcc atcactaagg tttttgaatt
     1561 aaatctttta tctgttttag gaactctagt tttcgctcta ttacttggtt atgtctcagc
     1621 ctacttcccg gcgcgtaaga ttagtaaaat ggatcctgta gaatcgttac gctatgaata
     1681 ataagggatt ccttgattta cagtcttaaa aagaaactca catagaaaaa caatggccat
     1741 acttgcaatt caactcaaat agttgtataa taggtttgtt gaaaggtgat ttgtagtgat
     1801 tcaagttact cttttcacaa taaaaatttc atgattttca taaggaggaa atcactaatg
     1861 gtagttaaag ttggtattaa cggtttcgga cgtatcggtc gtcttgcttt ccgtcgtatc
     1921 caaaacgtag aaggtgttga agttacacgc atcaacgacc ttacagatcc agttatgctt
     1981 gcacacttgt tgaaatacga cacaactcaa ggtcgtttcg acggtactgt tgaagttaaa
     2041 gaaggtggat tcgaagttaa cggtaaattc gttaaagttt ctgctgaacg tgatccagag
     2101 caaatcgact gggctactga cggtgtagaa atcgttcttg aagctactgg tttctttgct
     2161 aagaaagaag cagctgaaaa acaccttaaa ggtggagcta aaaaagttgt tatcactgct
     2221 cctggtggaa acgacgttaa aacagttgta ttcaacacta accacgacgt tcttgacggt
     2281 actgaaacag ttatctcagg tgcttcatgt actacaaact gcttggctcc aatggctaaa
     2341 gctcttcaag ataactttgg tgttgttgaa ggattgatga ctactatcca cgcttacact
     2401 ggtgaccaaa tgatccttga cggaccacac cgtggtggtg accttcgccg tgctcgcgct
     2461 ggtgctgcaa acatcgttcc taactcaact ggtgctgcta aagctatcgg tcttgtaatc
     2521 ccagaattga acggtaaact tgacggatct gcacaacgcg ttccaactcc aactggatca
     2581 gttactgaat tggtagcagt tcttgaaaag aacgttactg ttgatgaagt gaacgcagct
     2641 atgaaagcag cttcaaacga atcatacggt tacacagaag atccaatcgt atcttcagat
     2701 atcgtaggta tgtcttacgg ttcattgttt gacgcaactc aaactaaagt tcttgacgtt
     2761 gacggtaaac aattggttaa agttgtatca tggtacgaca acgaaatgtc atacactgca
     2821 caacttgttc gtactcttga atacttcgca aaaattgcta aataattctt gagttgatag
     2881 aaagcaaggc tttgtggtct tgctttttat atggaaaaat ggatgacacg atcatccatt
     2941 cttttttaat tctttttcaa atgtatttga aagggtagtg aaagttagct tctctaaagt
     3001 aagtgggtgg gtaaaggaaa gtcggaaggc atgaagcata agccggcttg tctttgattt
     3061 actattatag agagggtctc ccaggatagg aagattatga tgcgaaaggt gcacacgaat
     3121 ctgatgggtt cgccctgtct ttagcttgca atgagccaag gaagtcttgt ttgagaattg
     3181 ctttaatctg cttacatgcg tttcagcata ttgcccattt tttgcatcaa ctattctttt
     3241 tctacgatca tggcgatcac gtccaatttt gtctctgaaa acaagttctt ttctgttgat
     3301 atttccatca actagagccc aatattctct agaaatctct tttttctcca ataagcgatt
     3361 gagaatgggc aggataaaag gatttttggc aaagagaact aagccactgg tttccatgtc
     3421 cagacgatga acgacatagc aggtttggcc aacataggta ctgacatggt taagaagggc
     3481 aatttcgttt ggttgattac catgcgtttt catcccctct ggtttgttta caataatcaa
     3541 gtgttgatct tgataaactt cctgcactaa gtctgggttg ccccaaggga tcgtcttttg
     3601 ggaataatct tcctcgtcaa aagtcaactg gcaaacatct ccaggattta cgatttcctt
     3661 ccagtggact tcttcttgat ttatcaaaat atgtttcttg attctcaaaa aatgacggat
     3721 ttttctaggg atgaggagtt gttcctcaag taattgcttt actgtcattt gaggtagaga
     3781 ggcgggtaat gtaaatgtga attgcataca gatattgtaa caaaaaaagc cctatttgga
     3841 taggaaatag ctaaattctt gtcttcctat gatgaagatg ataaaataaa cgcatgaaat
     3901 tagataaatt atttgagaaa tttctttctc tttttaaaaa agaaacaagt gaactagagg
     3961 actctgattc tactatctta cgtcgctctc gtagtgatcg aaaaaaatta gcccaagtag
     4021 gtccgattcg aaaattctgg cgtcgttatc atctaacaaa gattatcctt atactaggtt
     4081 tgagtgcagg cttgctagtt ggaatctatt tgtttgctgt agccaagtcg accaatgtca
     4141 atgatttgca aaatgccttg aaaactcgga ctcttatttt tgaccgtgaa gaaaaagagg
     4201 ctggtgcctt gtctggtcaa aagggaacct atgttgagct gactgacatc agtaaaaact
     4261 tgcagaatgc tgttattgcg acagaagacc gttctttcta taaaaatgac gggattaact
     4321 atggccgttt tttcttggct attgtcactg ctggacgttc aggtggtggc tctaccatta
     4381 cccaacagct ggctaaaaac gcctatttat cgcaggatca aactgttgag agaaaagcga
     4441 aagaattttt ccttgcctta gaattaagca aaaaatatag taaggagcaa attctaacca
     4501 tgtaccttaa caacgcttat tttggaaatg gtgtgtgggg tgtagaagat gcgagtaaga
     4561 aatactttgg agtttctgca tcagaagtga gtctggatca agctgcgact ctggcaggga
     4621 tgctcaaggg gccggaactg tataatccct tgaattccgt agaagattct actaatcggc
     4681 gcgatactgt cttgcagaat atggttgcag caggatatat tgataaaaac caagaaaccg
     4741 aagctgctga agttgatatg acttcgcaat tgcacgataa gtatgaagga aaaatctcag
     4801 attaccgtta cccctcttat tttgatgcgg tggttaatga agctgtttcc aagtataatc
     4861 taacagagga agagattgtc aataatggct accgcattta cacagagctg gaccaaaact
     4921 accaagcaaa tatgcagatt gtttatgaaa acacatcgct atttccgagg gcagaggatg
     4981 gaacgtttgc tcaatcagga agtgtagctc tcgaaccgaa aacaggggga gttcgtggag
     5041 ttgtcggtca agttgctgac aatgataaaa ctggattccg gaatttcaac tatgcaaccc
     5101 aatcaaagcg tagtcctggt tctacaatta agcctttagt tgtttatacg ccagcagttg
     5161 aagcaggctg ggctttgaat aagcagttgg ataaccatac catgcagtat gacagctata
     5221 aggttgataa ctatgcaggg atcaaaacga gtcgagaagt tcctatgtat caagccttgg
     5281 cagaatcgct taatctacct gctgttgcca ctgttaatga tttgggtgtc gataaggctt
     5341 ttgaggcagg cgaaaaattc ggactcaaca tggaaaaggt cgaccgtgtt cttggtgtcg
     5401 ccttgggaag cggtgttgaa accaaccctc ttcaaatggc tcaagcatac gctgcctttg
     5461 caaatgaagg tttaatgcct gaagctcatt ttattagtag aattgaaaat gctagtggac
     5521 aagttattgc gagtcataaa aattcacaaa aacgggtgat tgataagtct gtagctgaca
     5581 agatgaccag tatgatgttg gggactttca ccaacggtac cggtattagt tcatcgcctg
     5641 cagactatat tatggcaggg aaaactggaa caactgaagc agttttcaat ccggagtaca
     5701 caagtgacca gtgggtaatt ggttatactc cggatgtagt gattagccac tggcttggct
     5761 ttccgaccac tgatgaaaat cactatctag ctggctctac ttcaaacggt gcagctcatg
     5821 tctttagaaa cattgccaat accattttac cttatacgcc aggaagtacc tttacggttg
     5881 aaaatgctta taagcaaaat ggaattgcac cagccaatac aaaaagacaa gtacaaacca
     5941 atgataatag ccagacagat gataatttgt ctgatattcg agggcgtgcg caaagtctag
     6001 tagatgaggc tagccgggct atctcagatg cgaagattaa ggaaaaggct caaacaatat
     6061 gggattcgat agtcaatcta tttcgctaag at
//
LOCUS       DAVKMU010000081         5963 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKMU010000081 DAVKMU010000000
VERSION     DAVKMU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.081"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            67..309
                     /locus_tag="VW343_001969"
     CDS             67..309
                     /locus_tag="VW343_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9756467.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            311..565
                     /locus_tag="VW343_001970"
     CDS             311..565
                     /locus_tag="VW343_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9756468.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHWRDIY"
     gene            612..1264
                     /locus_tag="VW343_001971"
                     /pseudo
     CDS             612..1264
                     /locus_tag="VW343_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
     gene            complement(1524..2510)
                     /locus_tag="VW343_001972"
     CDS             complement(1524..2510)
                     /locus_tag="VW343_001972"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9756469.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            2704..5172
                     /gene="gyrA"
                     /locus_tag="VW343_001973"
     CDS             2704..5172
                     /gene="gyrA"
                     /locus_tag="VW343_001973"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9756470.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            5172..5915
                     /gene="srtA"
                     /locus_tag="VW343_001974"
     CDS             5172..5915
                     /gene="srtA"
                     /locus_tag="VW343_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9756471.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         1875 a          981 c         1297 g         1810 t
ORIGIN      
        1 tttatattga ctgaacacct aaaaagtaat acaatggtgt taccattaaa aaagggagca
       61 caaaagatga ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca
      121 atggctaagt ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc
      181 ctagaagatg aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac
      241 ttggaaaaag gagttgaacc cattacttgg gaagaaatga tgcatgattt aggcttgaag
      301 gatgaataat ttgtataaat tagttccaac aagacgtttt atcaagcaat tgaaaaaatt
      361 ggaccgttat acgcagaagc taattacaaa ctatttacaa accaatgttt tggaagaccc
      421 aagacgacac ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg
      481 taattatcga gttatcgtac aaattgtaga tgatgaatta gttgttgcta ctctagaagt
      541 tggtcattgg agagatattt attgaattaa agctgagtaa taacagtata gaaagtaagg
      601 atagtaaaaa tttgaataaa aaatggttat ttgcagatta ttatgatata acaattattt
      661 tacttgcttt aatctctgtt attctggtat tacttggatt tgctgagatg attgatttgg
      721 acaatccgcc ttatagtatt attgatttag taatctgggg tgtttttgta attgattata
      781 gttggcgctt ttttattact aaaagaaaat ggcgctttat tcttgaaaat atttttgatt
      841 tattgactat tctcccttta aatgctattt ttacagtgtt tcgattagga cgtatcttcc
      901 gcttagcaag gctgacaaaa ttactgaaac tgactcgttt acttagaata attggactta
      961 caggtaaatt agaaagaaaa attagtagat tcttacgaac aaatggattg atttatatct
     1021 tgtatgttaa tatctttatt gttctagtag ggagttcgat tttatccgtt gttgaagaaa
     1081 aatccttctc agatagtctt tggtgggctc ttgtgacagt aactactgtt ggttatggtg
     1141 atattgttcc agtttccctt tttgggaaga cgaattatcg agccaaagaa tattaataga
     1201 gaaacaatcc gagaagatag aagaactgca tcggatgata caagatttgc ttgaaaaaac
     1261 ataataaaaa taaagatgag acaaagtttg agttgaggag aaatcaagta agttttgaca
     1321 caatagaacg catattatca ggtataaaaa cctgatacta tgcgttttat agagaaatga
     1381 aataaaatct gaacaaatca attaggaatt taaagcattt tataacaacg ttttagagta
     1441 atggggggct atttcaactt caacctacta taatacagaa aaaaacaact ccctgataat
     1501 tcaaggagtt gtctatagtt aaattagttt ttagaagctt cttggaattc tgggtttttc
     1561 catgcttcgt caatgatagc ttgcaattct ttagcagatg cttgcatttt ttgagtttct
     1621 gcgtcgttca atgggatatt tactggacga acaataccat gtgcaccaac aacagctggt
     1681 tgaccgataa agacgttctc aactccgtat tgaccttctt ggaatactga aagtggaagt
     1741 actgcatttt catcgtcaag gattgcttta gtgatacgag caagggctac tgcgataccg
     1801 tagtatgttg cacctttttt gttgatgatt gtgtaggctg catcacgaac accttcgaac
     1861 aattcaatca attcagcttc ttgaacattt tgagtatctt taaggaattc ttcaaggttt
     1921 acaccagcga tgttagcgtg tgaccaaaca gcgaactcag agtcaccgtg ttcacccatg
     1981 atgtaggcgt gaactgaacg agcatctacg tccaattttt cagcaagtgc ttgacggaaa
     2041 cgagctgagt caagtgaagt acctgaaccg ataacgcgtt ctttagggaa accagagaat
     2101 ttccaagttg agtaagtcaa aacgtcaact gggttagcag caacaaggaa gatacctttg
     2161 aaacctgatt caacaacttg agttacgatt gatttgttga tagcaaggtt tttacctaca
     2221 aggtcaagac gagtttcacc tggtttttga ggcgcacctg cagtgatcac aacaaggtca
     2281 gcgtctgcac agtcagagta ttgagctgca tagatttttt taggtgaagt gaaggcaagg
     2341 gcgtgactaa ggtcaagcgc atcaccaaca gctttttcat gcaattgtgg aatttcgata
     2401 attccaagct cttgtgcaat tccttggtta acaagtgcaa aagcgtaaga tgaacctaca
     2461 gcaccatcac cgacaaggat aacttttttg tgttgtttag ttgaagtcat tgttttaaac
     2521 atctccttaa ttttattagg ggattttccc tagacaactt cattctatca cttttaaaaa
     2581 actttgtcac gaatatgcct tatagttctc gatgtaaacg ttttagtggt ttagaggctg
     2641 aaatagatgg gaatttatgg tataatgttg ttacttacta attgtgaaat gaggcattta
     2701 ttaatgcagg ataaaaattt agtgaatgtc aatctgacaa aggagatgaa ggcaagtttt
     2761 atcgactacg ccatgagtgt tatcgtagcg cgagctcttc ctgatgttcg agatggctta
     2821 aaacctgttc accgtcgcat tctttacgga atgaatgaat tgggtgtgac cccagacaaa
     2881 ccccataaaa aatctgctcg tattacaggg gatgtcatgg gtaaatatca cccacacggg
     2941 gattcctcta tttatgaagc catggtccgt atggctcaat ggtggagcta ccgttacatg
     3001 cttgtagatg gtcatgggaa ttttggttcc atggatggag atagtgctgc cgctcaacgt
     3061 tataccgagg cacgtatgag caagattgct ctggaaatgc ttcgtgatat caacaaaaat
     3121 acagttgatt tcgttgataa ctatgatgcc aatgaacggg aacccttggt cttgccagcg
     3181 cgttttccaa accttttggt taatggagca actggtatcg cggttgggat ggcaaccaat
     3241 attccacctc ataatctggg tgaaaccatt gatgcagtga agttggtcat ggataatcct
     3301 gaagtgacta ccaaggactt gatggaagtc ttgcctggac cagattttcc aactggtgct
     3361 cttgtcatgg ggaaatcagg tatccataag gcttatgaaa caggtaaagg ttcgattgtc
     3421 ctacgttctc gtacagagat tgaaacgact aagactggtc gtgagcgcat cgttgtaaca
     3481 gaatttcctt acatggtcaa taaaaccaag gtgcatgagc atattgttcg cttggttcag
     3541 gaaaaacgca ttgagggtat cacagcagta cgtgatgagt caaaccgtga aggtgttcga
     3601 tttgttattg aagtcaagcg cgacgcctca gccaatgtta ttctcaataa cctcttcaaa
     3661 atgacccaaa tgcaaaccaa ttttggtttc aatatgctcg ctatccaaaa tggtataccg
     3721 aaaattttgt ctcttcgtca gattttggat gcttatatcg agcaccaaaa agaagtggtt
     3781 gttcgtcgta cacgttttga taaggaaaaa gcggaagcgc gcgctcatat cttagaaggt
     3841 ctcttgattg cgctagacca tatcgacgaa gtgattcgta tcatccgtgc tagtgaaacg
     3901 gatgcggaag ctcaagctga gttgatgagc aagtttaagc tttctgaacg tcaaagtcaa
     3961 gctatccttg atatgcgtct tcgtcgtttg acaggtttgg aacgcgataa gattcaatct
     4021 gagtatgatg acctcttggc tctgattgcg gatttagcag atattcttgc taagcctgaa
     4081 cgtgtttctc aaattatcaa agacgaattg gatgaagtta aacgtaaatt ttctgataag
     4141 cgccgtacag agttgatgat tggacaggtc ttgagtctcg aggatgagga cttgattgaa
     4201 gaatcggatg tcttgattac cctttctaac agaggctaca ttaagcgttt ggatcaggac
     4261 gagttcactg ctcaaaaacg tgggggtcgt ggtgtccaag gaacgggagt gaaagatgat
     4321 gactttgttc gtgagttagt gtcaactagc acccatgatc atctgctctt cttcacaaac
     4381 aagggacgtg tctatcgtct taaaggttat gaaattcctg agtatggtcg gactgccaaa
     4441 gggctaccag tagtcaatct cttgaaattg gatgaagacg aaagtattca gacggttatc
     4501 aatgttgagt ctgatcgcag tgatgatgct tatctcttct ttacaacccg tcacggtatt
     4561 gtgaagagaa ctagtgttaa ggagtttgcc aatattcgtc aaaatggtct caaagcgctg
     4621 aatttaaagg atgaagatga gttaatcaat gtcttgttgg cagaaggaga tatggatatt
     4681 atcattggta ccaagtttgg ttatgcagtt cgctttaatc aatcagccgt tcgtggtatg
     4741 agtcgtatcg ccactggtgt gaaaggtgtt aaccttcgtg aaggagacac agttgttggt
     4801 gccagcttga ttactgatca agatgaggtt cttattatca cagaaaaagg atatggtaag
     4861 cgtacagtcg ctactgaata cccaacaaaa ggtcgtggtg gtaagggaat gcagacagct
     4921 aaaattaccg aaaaaaatgg cttgctggcc ggtcttatga ctgttcaagg ggatgaggat
     4981 ttgatgatta tcactgatac aggtgtcatg attcgaacca atcttgccaa tatttcacaa
     5041 acaggacgtg caactatggg agttaaagta atgcgcctgg atcaagatgc tcagatagtg
     5101 actttcacaa cggttgcggt ggcagaaaaa gaagaagttg ggacagaaaa cgaaacagaa
     5161 ggtgaagcat aatgtctcaa aaaaataata aaaagaaaaa caagcgaaaa aatctgctga
     5221 caaatatcct agcaggattt ctgatattac tgtcactggc tttgattttt aatactcaaa
     5281 ttcgaaatat tttcatagtc tggaatacca ataagtatca agttagccag gtatcaaaag
     5341 aaaaattaga agaaaatcag gatacagaag gcaattttga ctttgattct gtcaaagcta
     5401 tctcttcgga agctgttcta acttctcaat gggatgctca aaaattacca gttattgggg
     5461 gaattgcaat tcctgaattg gaaatgaatt tgccgatttt taaaggactt gataatgtta
     5521 atctcttcta cggagctggt acaatgaaac gcgagcaagt aatgggagaa ggaaattata
     5581 gtctagctag tcaccatatc tttggtgttg ataatgctaa taaaatgtta ttttctcctt
     5641 tagataatgc taaaaatggc atgaagattt atctaactga taaaaataaa gtttatactt
     5701 atgaaatacg tgaagtcaaa cgtgtgacac cagatcgtgt tgatgaagtt gatgatagag
     5761 atggggtcaa tgaaatcaca ttagtaacct gtgaagacct tgctgctaca gaacgtatta
     5821 ttgtcaaagg tgatttgaaa gaaacaaaag attattcaca aacatctgat gaaatcctaa
     5881 cagctttcaa tcaaccatat aaacaatttt attaatacaa atcagtgaaa tcatggattt
     5941 cactgatttt ttaagatttt cat
//
LOCUS       DAVKMU010000082         5939 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKMU010000082 DAVKMU010000000
VERSION     DAVKMU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.082"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            411..632
                     /locus_tag="VW343_001975"
                     /pseudo
     CDS             411..632
                     /locus_tag="VW343_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(919..1401)
                     /locus_tag="VW343_001976"
     CDS             complement(919..1401)
                     /locus_tag="VW343_001976"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9756472.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(1496..2980)
                     /locus_tag="VW343_001977"
     CDS             complement(1496..2980)
                     /locus_tag="VW343_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9756473.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3133..4740
                     /locus_tag="VW343_001978"
     CDS             3133..4740
                     /locus_tag="VW343_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9756474.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDELIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            4905..5072
                     /locus_tag="VW343_001979"
     CDS             4905..5072
                     /locus_tag="VW343_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU9756475.1"
                     /translation="MKKRILSAGLTFTSALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(5065..5586)
                     /locus_tag="VW343_001980"
                     /pseudo
     CDS             complement(5065..5586)
                     /locus_tag="VW343_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1752 a         1117 c         1322 g         1748 t
ORIGIN      
        1 aactggttcc ttttgaggta cgacgggcat gtcgtatagt aggttgaaac tagaatagta
       61 cacatctact tctaaaacat ttttagaaat cgatttgact gtcctgatcg atttgtcctg
      121 ttcttatttc attttactat atatctgagg tgtaagtcct cggtgggcac ctgttaccgg
      181 tgaacccaat agcgattccc aagcctgact atcgtgaggt agcggattaa aacggtctgg
      241 ggatagaccg ttttaagtct gacgctggaa ataagaatcg tcagaggaag ggatagcgaa
      301 ctcgtggctc tacgaacagg aacgtgatag taaggcgtat atagcggata agggggcctc
      361 aaactctaaa gtccaaaaag gtagtcgtaa cctatatgcg taaatcacga gagcaactga
      421 atttgtccat tcgaggatgg ataaactatt tttcattggg aaatatgaaa agtatagtca
      481 ccagcataga tgagcgtttg catactcgcc tacgagtgat tatctggaag caatggaaga
      541 agaaatcgag acgattatgg ggattgctta agttaggggt tcctaaatgg atagcagata
      601 aggtatctgg ctggggcgac cactatcaat tatagtaaaa tgaaataaga atagaacaaa
      661 tcgatcagga tagtcaaatc gatttctaac aatgttttag aagcagaggt gaactattct
      721 agtttcaatc cactatagta gctcagaagt cggtacttaa acgtgctata tcaaaaccag
      781 tccttgaaaa acgtggattg gtttcgtgtt tggattatta ccttgaacga catgcgttaa
      841 aaagttagtt gaaccgccgt atgccgaacg gtacgtacgg tggtgtgaga ggggctagag
      901 attatcccct actcgatttt aaatcacatg acgttcaaag gcatcatctg aaatcccttg
      961 ttccaagatg agttttgccc attctttagc agagaagagg ctgtggtcct tgtagtttcc
     1021 gcaagattcg atggttgtcc ctgggacatc ttcccaagta gtagtttcag cgatttcctt
     1081 gagcgaatcc ttgataacag ctgcgatttt agcactggtg tgacgtcccc acataatcat
     1141 gtggaagcct gtgcggcaac caaatggtga acagtcaatc atgccgtcaa tgcgggtacg
     1201 gatgagtttg gctaagaggt gctcgatagt gtgaaggccg gcagtaggga tagagtcttc
     1261 gtttggttgc accaagcgaa tatcataatt ggagatgatg tctccttttg gtcctgtttc
     1321 ttccccaatc aagcgaacat agggtgcttt gacaatggtg tggtcaagtt caaaactttc
     1381 gacaataact tcttttgaca tggtaaatcc tttcagtttt cttctctcat tatatcataa
     1441 aggttgctcc tgagacagag agaaaacctc tccgaggctg gagaggttga aatctttact
     1501 tacgatataa gcggtcgtat tggtagtatg ggtcaaaggt tacgttgata cccagtttac
     1561 gaaggacatt cttgtcttca tcagtcaaga tgatggttga gtgggcttcg cttcctttga
     1621 ggttgccgag ttcttccata gcgcgggcag catcaggatt ttctgtagct gtgatagcaa
     1681 gtgcaatcag gatttcattt gaatgaaggc gtggattgcg gctaccgaga tgatcgattt
     1741 taagaccttg gattggctta acaacttcag gctcgattag ttttacttct ttagcgatgt
     1801 cagctgattt tttgatggcg ttgatcaagg cagcggctgt aggcccaaag agttctgagt
     1861 tcttaccagt gacgatttcc ccatttggca attcaaaggc tagggctggt ccaccagttt
     1921 cttctgcttt ttggcgcgca acgacagcaa ccttacggtc tgcaggtgtg ataccgaggt
     1981 cgttcatgag caactcaatt ttcttgacgg cagcttcgcc aactttttca gctttgaagt
     2041 caagaactgt ttgatagtaa cggcggatga tttcttgttt agaagcttcg acagcagcct
     2101 cgtcatctgt aatagcgaaa ccaaccatgt tgacacccat atctgtcggt gaagcgtatg
     2161 gtgattttcc gagaatacgt tccaacatgc gcttgagcac tgggaagatt tcgatatcac
     2221 ggttgtagtt gacagtggtt tctccatagg tttgaagatg gaaggggtca atcatgttga
     2281 catcatcaag gtcagctgtg gcagcttcat aagccaagtt aactggatga tgaaggggaa
     2341 gattccaaac agggaaggtt tcaaatttag cgtagccaga cttgatgcca ttgatttggt
     2401 cgtggtacat attggacata cacgttgcca attttccaga accgggtcca ggagcggtta
     2461 cgacaatcaa gttgcgactg gttttgatgt agtcgttttt gcccatgcct tctggggaaa
     2521 tgatgtgatc catatccgtc ggatatcctt tgattggata atgaagataa gaatcaattc
     2581 cgtttttctc aagttggttg cggaaggcat ctgcagcgag ttggccagcg tattgtgtaa
     2641 tgacaacgga accaacaaaa atccctaatt cattgaattt gtcaatcaaa cgaagaactt
     2701 cttggtcata agaaatgcct aagtcgccac gtgctttgga atgttcaatg ttgctagcat
     2761 taatggcaat cacaacctca acctgctctt tcaattcttg caagagcttg attttgttgt
     2821 caggttcata accaggaagg acacgagcag cgtggaaatc ttctaacatt ttaccgccaa
     2881 actctaagta gagcttgccg tcaaattggt taatgcgctc taagatgtgg tcgcgttgta
     2941 aattcaaata ctgttcagaa ctaaaagctt gttttttcat ttttttacct ctggattcta
     3001 ttataataaa aaattggaag ttaggaaact acggagctaa aaaagaaatt aaaaagatta
     3061 agcaaacgct tgcacaaaat tttaaaaagt gctatcatat actatagatt attaaaataa
     3121 tgaggtaaac agatgcaaga aaaatggtgg cataatgccg tagtctatca agtctatcca
     3181 aagagtttta tggatagtaa tggagatgga gttggtgatt tgccaggtat taccagtaag
     3241 ttggactatc tagctaagct aggaatcaca gcgatttggc tttctcccgt ttatgacagc
     3301 cctatggatg ataatggcta tgatattgct gattatcaag cgattgcggc tatttttgga
     3361 accatggagg acatggatga actgattgca gaagctaaga agcgtgatat ccgtatcatc
     3421 atggacttgg tggtcaatca tacctcggat gagcatgcct ggtttgtaga ggcctgtgaa
     3481 aatcctaata gccctgagcg agactactat atctggcggg atgagcctaa cgatttagat
     3541 tctatcttta gtgggtctgc ttgggaatac gatgaaaagt caggtcaata ctatctccac
     3601 tttttcagca agaaacagcc ggatctcaac tgggaaaatg aaaaacttcg ccagaaaatt
     3661 tatgagatga tgaacttctg gattgataag ggtattggtg gtttccgtat ggatgttatt
     3721 gacatgattg gcaaaattcc tgacgagaag gtagtcaata atggtcctat gctccatccc
     3781 tatctcaagg aaatgaatca ggcgaccttt ggagataagg atctcttgac agtaggggag
     3841 acttggggag caacgccaga gattgccaaa ctctactctg atccaaaggg gcaagaattg
     3901 tctatggtct tccagtttga acatatcggt cttcagtatc aggaaggtca acctaaatgg
     3961 cactatcaaa aagagctgaa tatcgctaag ttaaaagaaa tcttcaacaa atggcagaca
     4021 gagttaggag ttgaggacgg ctggaattcc ctcttctgga acaaccatga cctccctcgt
     4081 attgtctcaa tctggggaaa tgaccaagaa taccgcgaaa aatctgccaa agcctttgca
     4141 atcttacttc atctcatgag aggaactcct tatatctacc aaggtgagga gattgggatg
     4201 accaactatc cgtttgaaac actggatcaa gtagaagata ttgaatctct caactatgcg
     4261 cgtgaggctc ttgaaaaagg tgttccgatt gaagaaatca tggacagtat ccgtgttatt
     4321 ggacgtgaca atgcccgtac ccctatgcaa tgggacgaga gcaaaaacgc tggtttctca
     4381 acaggtcaac cttggttggc agttaatcca aattacgaga tgatcaacgt ccaagaagcg
     4441 ctggcaaatc cagattctat tttctatacc tatcagaaac tggtccaaat tcgcaaggag
     4501 aatagctggc taattcgagc tgactttgaa ttgcttgata cggctgataa ggtctttgct
     4561 tatatacgta aggatggcga ccgtcgcttc ctagttgtgg ctaacttgtc caatgaagag
     4621 caagacttga cagtagaagg aaaagtcaaa tctgtcttga ttgaaaacac cctagctcaa
     4681 gaagtctttg aaaaacaaat cttagttcca tgggatgctt tctgtgtgga attactataa
     4741 atattttttg cagaaaaatt taaaattgaa atcgtataaa aacaagggag gactgtataa
     4801 aaggcagaaa tcctttgttt tttataacca aggtttataa accttcattc tcgaaattca
     4861 attaacttta caaattccca ctattaagga gaaagaagac gaacataaag aagcgtatcc
     4921 ttagtgcagg cctgactttt acatctgctt tgcttttagc tgcttgcgac caatcaggtt
     4981 cagatacaaa aacttactca tcaaccttta gtggaaatcc aactacattt aactatctat
     5041 tagactatta cgctgataat atagtcaatt gaaacaagaa caagacaaaa gagcctcata
     5101 aaaggtattg caacttggta ataccttttt gaggtgcttt ttgatatgag cccatgtttt
     5161 ctcaatagga ttgtactcag gtgagtaggg aggaagaggt aaaagtttat gcccaaactc
     5221 ttcgcataaa agttctagct tccccattct atggaatctt gcattatcca taataataac
     5281 cgatggtgtg gttaatgttg gtaagaaaaa cttctgaaac catacttcaa aaaagtcgct
     5341 cgtcatcatc tcttcgtaag tcattggagc gattaattca ccatttgtta gacctgcaac
     5401 caaagaaatc ctctgatatc ttcttccaga tactttgcct cttcttaact gaccttttaa
     5461 tgagcgacca tattctcgat aaaaataagt atcgaatcct gtttcatcaa tctaaacagg
     5521 tgctaggtgc tttaaactat taaaattctt aagaaataag gctacttttt ctgggtcttg
     5581 ttcataatta gattgaaact agagtagtag acctctgctt ctaaaacatt gttagaaatc
     5641 gatttgactg tcctgatcga tttgtcatgt tcttatttca ttttactata tttttgtttc
     5701 gcgggaagtc tactaagata cttaaagatg cagatagtaa aaaaaatgtg tcaatcgaaa
     5761 gtgattatgt ccccaaaaaa tggagagttt gagggcaatt attttgattt catttgacac
     5821 gaggaaccct tgaagctgtt ttgttagcta aaccaaggct agtcttagcc ttggctcgcc
     5881 agcctaacag ctccaagcct cttatcaaat gaaatacttg tcttacactc ctcactttc
//
LOCUS       DAVKMU010000083         5852 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKMU010000083 DAVKMU010000000
VERSION     DAVKMU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.083"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            109..1809
                     /locus_tag="VW343_001981"
     CDS             109..1809
                     /locus_tag="VW343_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9756476.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1802..2278
                     /gene="ilvN"
                     /locus_tag="VW343_001982"
     CDS             1802..2278
                     /gene="ilvN"
                     /locus_tag="VW343_001982"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9756477.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2344..3366
                     /gene="ilvC"
                     /locus_tag="VW343_001983"
     CDS             2344..3366
                     /gene="ilvC"
                     /locus_tag="VW343_001983"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9756478.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3473..3709
                     /locus_tag="VW343_001984"
     CDS             3473..3709
                     /locus_tag="VW343_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756479.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     SREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            3947..4303
                     /locus_tag="VW343_001985"
     CDS             3947..4303
                     /locus_tag="VW343_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9756480.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            4353..5603
                     /gene="ilvA"
                     /locus_tag="VW343_001986"
     CDS             4353..5603
                     /gene="ilvA"
                     /locus_tag="VW343_001986"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9756481.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1810 a          988 c         1337 g         1717 t
ORIGIN      
        1 taaacgttaa aatagaaaat tcagaaaatt tcttctttta tcttgaaaaa ttttgaaaaa
       61 atggtatgat agtaacaagt tatttttaag aggaaagaaa ggggaacaat ggagaaaatc
      121 agtttagaat ctcctaagac ggggtcggac ctagttttgg aaacacttcg tgatttagga
      181 gttgatacca tctttggtta tcctggtggt gcggttttgc ctttttatga tgcgatatat
      241 aattttaaag gcattcgcca cattctaggg cgccatgagc aaggttgttt gcatgaagct
      301 gaaggttatg ccaaatcaac tggaaagttg ggtgttgccg tcgtcactag tggaccagga
      361 gcaacaaatg ccattacagg gattgcggat gccatgagcg atagcgttcc ccttttggtc
      421 tttacaggtc aggtggcgcg agcagggatt gggaaggatg cctttcagga ggcagacatc
      481 gtgggaatta ccatgccaat cactaagtac aattaccaag ttcgtgagac agctgatatt
      541 ccgcgtatca ttacggaagc tgtccatatc gcaactacag gccgtccagg gccagttgta
      601 attgacctac caaaagatat atctgcttta gaaacagact tcatttattc accagaagtg
      661 aatttaccaa gttatcagcc gactcttgag ccgaatgata tgcaaatcaa gaaaatcttg
      721 aagcaattgt ccaaggctaa aaagccagtc ttgttagctg gtggtggaat tagttatgct
      781 gaggctgcta cggaactaaa tgaatttgca gaacgctatc aaattccagt ggtaaccagt
      841 cttttgggac aaggaacgat tgcaacgagt cacccactct ttcttggaat gggaggcatg
      901 cacgggtcat tcgcagcaaa tattgccatg acggaagcgg actttatgat tagtattggt
      961 tctcgtttcg atgaccgttt gacggggaat cctaagactt tcgctaagaa tgctaaggtt
     1021 gcccacattg atattgaccc agctgagatt ggcaagatta tcagtgcaga cattcctgta
     1081 gttggagatg ctaagaaggc cttgcaaatg ttgctagcag aaccaacagt tcacaacaac
     1141 actgaaaagt ggattgagaa agtcactaaa gacaagaatc gtgttcgttc ttatgacaag
     1201 aaagagcgtg tggttcaacc gcaagcagtt attgaacgaa ttggtgaatt gacgaatgga
     1261 gatgccattg tggtaacaga cgttggtcaa caccaaatgt ggacagctca gtattatccc
     1321 taccaaaatg aacgtcagtt agtgacttca ggtggtttgg gaacaatggg ctttggaatt
     1381 ccagcagcaa tcggtgctaa aattgctaac ccagataagg aagtagtctt gtttgttggg
     1441 gatggtggtt tccaaatgac caaccaggag ttggctattt tgaatattta caaggtgcca
     1501 atcaaggtgg ttatgctgaa caatcattca cttggaatgg ttcgccagtg gcaggaatcc
     1561 ttctatgaag gcagaacatc agagtcggtc tttgataccc ttcctgattt ccaattgatg
     1621 gcgcaggctt atggtattaa aaactataag tttgataatc ctgagacctt ggctcaagac
     1681 cttgaagtca tcactgagga tgttcctatg ctaattgagg tagatatttc tcgtaaggaa
     1741 caggtgttac caatggtacc ggctggtaag agtaatcatg agatgttggg ggtgcagttc
     1801 catgcgtaga atgttaacag caaaactaca aaatcgttca ggagtcctca atcgctttac
     1861 aggtgtccta tctcgtcgtc aggttaatat tgaaagcatc tctgttggag caacagaaga
     1921 tccgaatgta tcgcgtatca ctattattat tgatgttgct tctcatgatg aagtggagca
     1981 aatcatcaaa cagctcaatc gtcagattga tgtgattcgc attcgagata ttacagacaa
     2041 gcctcatttg gagcgcgagg tgattttggt taagatgtca gcgccagctg agaagagagc
     2101 tgagatttta gcgattattc aacctttccg tgcaacagtg gtagacgtag cgccaagctc
     2161 gattaccatt cagatgacgg gaaatgcaga aaagagcgaa gccctattgc gagtcattcg
     2221 cccatacggt attcgcaata ttgctcgaac gggtgcaact ggatttaccc gcgattaaaa
     2281 atccaactta aatttattaa accagcctaa aaggcaataa ataatagaaa agagagaaaa
     2341 gctatgacag ttcaaatgga atatgaaaaa gatgttaaag tagcagcact tgacggtaaa
     2401 aaaatcgccg ttatcggtta tggttcacaa ggacatgcgc atgctcaaaa cttgcgtgat
     2461 tcaggtcgtg acgttattat cggtgtacgt ccaggtaaat cttttgataa agcaaaagaa
     2521 gatggatttg atacttacac agtagcagaa gctactaagt tggctgatgt tatcatgatc
     2581 ttggcgccag acgaaattca acaagaattg tacgaagcag aaatcgctcc aaacttggaa
     2641 gctggaaacg cagttggatt tgcccatggt ttcaacatcc actttgaatt tatcaaagtt
     2701 cctgcggatg tagatgtctt catgtgtgct cctaaaggac caggacactt ggtacgtcgt
     2761 acttacgaag aaggatttgg tgttccagct ctttatgcag tataccaaga tgcaacagga
     2821 aatgctaaaa acattgctat ggactggtgt aaaggtgttg gagcggctcg tgtaggtctt
     2881 cttgaaacaa cttacaaaga agaaactgaa gaagatttgt ttggtgaaca agctgtactt
     2941 tgtggtggtt tgactgccct tatcgaagca ggtttcgaag tcttgacaga agcaggttac
     3001 gctccagaat tggcttactt tgaagttctt cacgaaatga aattgatcgt tgacttgatc
     3061 tacgaaggtg gattcaagaa aatgcgtcaa tctatttcaa acactgctga atacggtgac
     3121 tatgtatcag gtccacgtgt aatcactgaa caagttaaag aaaatatgaa ggctgtcttg
     3181 gcagacatcc aaaatggtaa atttgcaaat gactttgtaa atgactataa agctggacgt
     3241 ccaaaattga ctgcttaccg tgaacaagca gctaaccttg aaattgaaaa agttggtgca
     3301 gaattgcgta aagcaatgcc attcgttggt aaaaacgacg atgatgcatt caaaatctat
     3361 aactaattag aaatatatag cgctggagat gattttatga aaaagattat gagaaaaact
     3421 gcatcgttat tattggttct agttgtataa tgtaattaca ccgtcggtaa tagtgctagc
     3481 agaccaaaat aaagcagatt ggtcgtatga tgaaaatgct gtaattaaca tttatgatga
     3541 tgctaatttt gaagatggta ggttgcatat gaactttgaa caattcttca aattggcaca
     3601 aatatctaga gaagaaggtc ttgaaattca ttctccgttt gagagagctg gtgcgactaa
     3661 atctgctcgt tatatagcga aatggatttt gagaaataaa aaacattaac aaatatagtt
     3721 ggtaaatcat taggacctaa atcagctgtt agattcggag aagctttatc ctatattgaa
     3781 ggtcctcttc gcagaataaa tgagacgata gatggcggtt tatatcaaat agagcaaatt
     3841 attgcatctg gattgaaaga atcgggttta aatgactgga ctgcgaaaac tttagcttca
     3901 gctattcgtg ggatattaga tgtacttatt taggggttga aatcatatga atattaccaa
     3961 tttgttttct atcaagacag gatgtgatga aactgatagg caactgcaaa aactattttt
     4021 tcagttggat ttacaattgg gagaattgac agatcaacta agaaaattag attctaattt
     4081 tgttcctcgt agtcaatttg tagacacgtt ggatttgaat gatgtagaat ataaagaaat
     4141 tttaaactat tttatcttcc atcgtaatga tagtgaagaa agtttggtag aatggttata
     4201 tgattggatt tccacaaatc gttatgaact tcctaaagag ttttcgattc gtatggctca
     4261 taaataccat gaaagtgtta ctgaagtttt cggagatgaa taactaaaaa acagtcatta
     4321 gtgactgttt tttatagaaa aagaggtttt atatgttaag ttcaaaagat ataatcaagg
     4381 ctcacaaggt cttgaacggt gtggttgtga atactccact ggattacgat cattatttat
     4441 cggagaagta tggtgctaag atttatttga aaaaagaaaa tgcccagcgt gttcgctcct
     4501 ttaaaattcg tggtgcctat tatgccattt cccagctcag caaggaagaa cgtgaacgtg
     4561 gggtagtctg cgcttctgcg ggaaatcatg cgcagggagt agcctatact tgtaatgaaa
     4621 tgaaaattcc tgctactatc tttatgccca ttactacgcc acaacaaaag attggtcagg
     4681 ttcgcttttt tggtggggat tttgtaacta ttaaactagt tggagatacc tttgatgcct
     4741 cagccaaagc agctcaagaa tttacagtct ctgaaaatcg tacctttatt gatccttttg
     4801 atgatgctca tgttcaagca ggtcaaggaa cagttgctta tgagatttta gaagaagctc
     4861 gaaaagaatc gattgatttt gatgctgtct tggttcctgt tggtggtggc ggtctcattg
     4921 ccggggtttc tacctatatc aaggaaacaa gtccagagat tgaggttatc ggagtagagg
     4981 cgaatggagc gcgttccatg aaagctgcct ttgaggctgg aggtccagtc aaactcaagg
     5041 aaattgataa atttgcggat gggattgctg tgcaaaaggt aggtcagttg acctatgaag
     5101 caactcgtca acatattcaa actttggtag gtgtcgatga gggattgatt tctgaaacct
     5161 tgattgacct ttactctaag caagggatag tcgcagaacc tgctggagca gctagtatcg
     5221 cctctttaga ggttttagct gaatatatta aggggaaaac catttgttgt atcatttctg
     5281 gaggaaataa tgatatcaac cgtatgccag aaatggaaga gcgtgccttg atttatgatg
     5341 gtatcaaaca ttactttgtg gtcaatttcc cacaacgtcc aggagctttg cgtgagtttg
     5401 taaatgatat cctggggcca aatgatgata tcacacgttt tgagtatatc aaacgagcta
     5461 gcaagggaac aggcccagta ttaattggga tcgctttagc agataagcat gattatgcag
     5521 gtttgattcg tagaatggaa ggttttgatc cagcttatat taacttaaat ggtaatgaaa
     5581 cgctttataa tatgcttgtc tgaggactaa taaaaaaata tcatattatt tgtacaactg
     5641 atgtatatgt gctataatga agatgaagaa aggggacgat tcctatggtt ttagaaaact
     5701 tatcgtgcaa ttcttacttc tgttcattat agcacttatg atgtttggct cagatttcca
     5761 tccttcattt tgatttccta tctattgaca agcatagtca cactgtcttt aatactcttc
     5821 gaaaatctct tcaaaccacg ttagctctat ct
//
LOCUS       DAVKMU010000084         5581 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKMU010000084 DAVKMU010000000
VERSION     DAVKMU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.084"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            121..1151
                     /locus_tag="VW343_001987"
                     /pseudo
     CDS             121..1151
                     /locus_tag="VW343_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1148..1366
                     /locus_tag="VW343_001988"
     CDS             1148..1366
                     /locus_tag="VW343_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756482.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETKPTDESGT"
     gene            complement(1423..1707)
                     /locus_tag="VW343_001989"
     CDS             complement(1423..1707)
                     /locus_tag="VW343_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9756483.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(1761..2696)
                     /locus_tag="VW343_001990"
     CDS             complement(1761..2696)
                     /locus_tag="VW343_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9756484.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            2800..3243
                     /locus_tag="VW343_001991"
     CDS             2800..3243
                     /locus_tag="VW343_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9756485.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSIGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVV"
     gene            3359..3625
                     /locus_tag="VW343_001992"
     CDS             3359..3625
                     /locus_tag="VW343_001992"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9756486.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            3622..3996
                     /gene="crcB"
                     /locus_tag="VW343_001993"
     CDS             3622..3996
                     /gene="crcB"
                     /locus_tag="VW343_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9756487.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            3990..4319
                     /gene="crcB"
                     /locus_tag="VW343_001994"
     CDS             3990..4319
                     /gene="crcB"
                     /locus_tag="VW343_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9756488.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            4438..4785
                     /gene="rplS"
                     /locus_tag="VW343_001995"
     CDS             4438..4785
                     /gene="rplS"
                     /locus_tag="VW343_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9756489.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            4827..4898
                     /locus_tag="VW343_001996"
     tRNA            4827..4898
                     /locus_tag="VW343_001996"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4860..4862,aa:Arg,seq:cct)
     gene            5065..5232
                     /locus_tag="VW343_001997"
     CDS             5065..5232
                     /locus_tag="VW343_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756490.1"
                     /translation="MSLIYSSAKKDFLYWNVLIFIMELPNDVKVQFYELRKKVQSFNQ
                     LSKRFGMDVSG"
BASE COUNT         1694 a          976 c         1161 g         1750 t
ORIGIN      
        1 tcagaaatca cttgttaatt tatccgtgat atgttagatt tgctagtaaa attatgacca
       61 ttttggagga aagcaatatt tataccagac taaaatcaaa aagcattata taatagtgat
      121 atgaaatcaa ctaaagaaga aatccaagcc atcaaaacac ttttaaaaga ctctcgtaca
      181 gctaaatatc ataaacgcct tcaaatcgtt ctatttcgtc tgatgggcaa atcttataaa
      241 gagattatag agcttttaga ctgtaatcaa acaacgattt ggcgaaatgt aaaaaaatat
      301 gaggagttcg gactcgactc tctccttcaa gaaacacgtg gtggtcgtaa ccatgcatat
      361 atgacagttg aggaagagaa agcctttctt gcccgccatt tgaaggctac agaggcagga
      421 gaatttgtta caatacctta tttcaggctt atatcgttcc tacacacgtg atgccttcta
      481 tcaactgttg aagcgccatg gttggcgaaa tattatgcca cgtccagaac atcctaagaa
      541 agcagacgct caaaccattg tcgcgtctaa aaataaaatc tcaattcaag aagaaaagaa
      601 agcgctttaa aaccagtaga ccttttcata aggttcgctc gatgtaccaa gatgaggctg
      661 gtttcggtag aatcagtaaa ctgggatctt gttggtctcc aataggagta ggtccacatg
      721 tccatagtca ctatatacga gaatttcgct attgttatgg agctgttgat gcccatacag
      781 gcgaatcatt tttcttaaga gctggtggat gtaatactga gtggatgaac gcctttttag
      841 aagagctttc acaagcttat ccagatgatt atcttttact cgttatggac aatgctatat
      901 ggcataaatc aagtacctta aagattccga ctaatattgg ctttgcattt attcctccgt
      961 acacaccaga gatgaacccc attgaacaag tgtggaaaga gattcgtaaa cgtggattta
     1021 aaaataaagc ctttcaaact ttggaagatg tcatgaatca actttaagat atcatacaag
     1081 gactggagaa ggaggtgata aagcccatcg ttaatcggag atggactaga atgctttgtg
     1141 aaagcagatg agtataagtc ctcgttttga aaccttagag caggccattg cctccaaaga
     1201 tttggaaaaa gtccgagaag cttttaagaa aatgaattcc acttggacca tcaatgaaag
     1261 tgtggttcgt gacaatagta cagctcatta tggacgtgtt gaaacagcta tttccttcct
     1321 gcctagtagc atggaaacca aacctacaga tgagtctgga acatgaaaac agcagttgat
     1381 ttcaagaaac aaaaaagaac agccgaaact gttctcttat cattatttga gaccgtattt
     1441 tttgttgaaa cgatccacgc gtccatctgc ttgagtgaac ttttgacgtc cagtgtagaa
     1501 tgggtgtgag tctgatgaaa tttccacacg gatcaatggg taagtttcgc cttcgaactc
     1561 aactgtttcg ttagagcgtt ttgttgaacc gctaaggaat tggtaaccag tagttgtgtc
     1621 catgaagaca actgggcgat attctggatg gatatctttt ttcattttgc aatatttcct
     1681 ttctgccatg gtctctttgc gagccataga ttgttaccat actagtctat cagattctga
     1741 aaagtttggc aagtatttta tcagttttta agcaagtttt ttaacttttg gtagatgatt
     1801 tcgttttctt ctaggctata ggaattagca ccacttgcta gagggtggcc tccaccatca
     1861 tgctccttgg cgatttcatt gataggatgg attttactgc gtaagcgaac tcggtagtgg
     1921 ccatcagcct gttcgacaaa aattccccag agactcactc tgtcaatgcg tccaggtgca
     1981 ccgacaatgg ctgcagtttc agcatcggtt acattgtatt gtttcaagac ttcttgactc
     2041 aggataacgc gagcagcacc attttcatcc acttccagat ggtcgtagat gtagccttgc
     2101 agtttagcaa ttttgtagct catagtgtcc attttgcgag tgagagccgc aaagtcaaag
     2161 ttatgttctc tcaaataagc agccaggcga agagtccgtg cagtggtaga agggtagagg
     2221 aagcgacctg tatcaccgac aattcctgca aagagcaact cagcatcgcg atctgccaag
     2281 gctagttggg ttgtttgggc aaatagggta atcatctcgc tagcgctact tgaactagta
     2341 tcgacccaag acaggtcacc gtatacatca tcatttggat ggtggtcaat cttaatgaga
     2401 aaatcacctt gactatagcg cttatcatcg atacgagcag tattagctgt atcacagacg
     2461 atgacaagtg cgccttggta ggctctatct tcaacaagat ccatctcagc catccaagta
     2521 agagttggtt catcaaaacc gacggctttg atggtttttt ctgggaaatg atgttccagc
     2581 aaggctttca atcccacctg acttcccaag gcatcagggt ctggtttcat atgacgatga
     2641 atgataatgg tgtcgtattc tttaattttt tctaaaattt gttggcaaat ctccataaat
     2701 aacctcaatt ctttctcatt tcattgtagc acaagaattt ttatttttaa aatgaaaaag
     2761 atgtgatata atggagaaag ataaattgag gaggacgata tggcattagc aaaaattgta
     2821 tttgccagta tgaccggtaa taccgaagaa attgcagata ttgtagcaga caaattacgt
     2881 gacttgggct tggatgttga tgttgatgaa tgtacaactg ttgacgcttc agacttcttg
     2941 gaagcagaca tcgctatcgt tgcgacctat acttatggtg atggtgaatt gccagatgag
     3001 atgatggact tctacgaaga cctagcagat ctcaacttga atggcaaaat ctacggagtg
     3061 gtcggctcag gagatacctt ctacgatgaa ttctgtaagg ctgttgatga ctttgaccgt
     3121 gtctttgtgt caataggagc agaaaaaggt tcagagtgtg ttaaagttga tctttctgcc
     3181 gaggaagaag atattgaacg cttggaacaa ttcgcagaag aattggttgc taaagtagta
     3241 taagcataaa agtgcgctga tgtaatcaat cagtgcattt ttcttatcga gagttgggat
     3301 tttactagcc taattgatgg ctttttgata caataattca aataaaggag gagactgtat
     3361 ggatttagat attattcgtc aagaaatcga tcaaatcgat gaccaaattg tcaaactgtt
     3421 agaagaacga atgcatttag ttgagggggt ggttgcttat aagaaagctt caggtaaacc
     3481 gattttagat actaagagag aagaagttat ttttgaaaag gttaggagtc gtgtagagga
     3541 caagcgctat caggagacta ttgtagcgac tttttcggac atactcaaac gttcgcgtga
     3601 ttatcaggat caaaatatca aatgaaaaaa gaacaatttt atccgctagg aatttttcta
     3661 gctgctatgt tgggcggact tgtccgatat ctagtttcca cctggttacc agccagtcca
     3721 gactttcctt ggggcactct ctttgtcaac tatctgggaa ttttctgctt gatttttctt
     3781 gtcaagggct atctggtcta taaggggact agtaagggct tgattttagc actggggacg
     3841 ggtttttgcg gaggtttaac aactttttcc agcctaatgc ttgatactgt gaagctgctt
     3901 gatacagggc gttatcttag tttagtcctg tatttgcttt tgagcatcgg tggaggccta
     3961 ctcttagctt attttctagg gaggaagaaa tggtaatcgt ctatcttgca atcgcctgtg
     4021 gcttgggagc actcgtacgg tatttctttt cccgctacaa tcaagcttct aaattgccac
     4081 tcggaacgct catagctaat cttttaggtt gttttttaat tggagtattc tacaatcatg
     4141 tagagtctaa ggaagtatat gctattctag caacaggatt ttgtggcggt ttaacaactt
     4201 tttcgacctt gaatgacgaa cttcaaagac tgttaagtga taagaaggtc ttttattctt
     4261 acctgacttt gacttacata ggtggtttgg ttgcgatttt tttaggaatt ttgctataaa
     4321 tttctttact tttttgtgaa aatttggtag actgtatctt gtgtgtgatg gacgcacaaa
     4381 aatacagttt atccgctgag gaaatttcct caagattgac aaagatagga gaataaaatg
     4441 aatccattaa tccaaagctt gactgaaggt caacttcgta cagatatccc atcattccgt
     4501 cctggtgaca ctgttcgtgt acacgcgaaa gttgtcgaag gtaaccgtga acgtatccag
     4561 atttttgaag gtgttgttat cgcacgtaaa ggtgctggaa tctcagaaaa ctacacagtt
     4621 cgtaaaatct ctaacggtgt aggtgttgag cgtatcttcc caatccacac tccacgtgtt
     4681 gaaaaaatcg aagttgttcg ttacggtaaa gtacgtcgtg cgaaattgta ctacttgcgt
     4741 gctcttcaag gtaaagcagc tcgtatcaaa gaaatccgtc gttaattcga ctaaaaaact
     4801 ctgcttttca gcagagtttt tatctagctc ccttagttca atggatataa caactccctc
     4861 ctaaggagta gttgcaggtt cgattcctgc agggggcatt tttagtaatg taaagaaaga
     4921 caaagaaaat tcaaaatgtt tatttgaagg caattttctc aagttcattt ttaaaaataa
     4981 tttagcattt ttaatttttt tgaccaaatt ttgaccaaaa attatatttt ggtcaatgat
     5041 tatagaataa aatggatcat aaaaatgtct ttgatttata gtagtgctaa aaaggacttc
     5101 ctttattgga atgtccttat ttttattatg gaattgccaa atgacgttaa ggttcagttc
     5161 tatgaactta gaaagaaagt acaaagcttt aatcagcttt caaaaagatt tggtatggat
     5221 gtttctggct aaggtatgtg acaaggtgaa ttgatcgtta ttgaacagag agcgttaaaa
     5281 aagggaagaa tcgttactat tccactgaat taaaacaaga aataaatcaa caaaagccta
     5341 tataataaca aatgtattaa aaataaaaag atttagttca gtgtaatgca gaaccaaatc
     5401 ttatggataa attatttgtc taatttttta ggaattatat ctctaaacaa tatcatttaa
     5461 caattaaatt gttagatcta gcttaaattt ctggtttaat tcatttattg tgtcactatc
     5521 attatattcc ttaaatattc tcatagcttt agaacattct tttttaccta actctatttg
     5581 a
//
LOCUS       DAVKMU010000085         5497 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKMU010000085 DAVKMU010000000
VERSION     DAVKMU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.085"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(81..713)
                     /locus_tag="VW343_001998"
     CDS             complement(81..713)
                     /locus_tag="VW343_001998"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9756491.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKDDQENWQEG"
     gene            complement(747..1448)
                     /gene="pyrF"
                     /locus_tag="VW343_001999"
     CDS             complement(747..1448)
                     /gene="pyrF"
                     /locus_tag="VW343_001999"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9756492.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            1769..1861
                     /locus_tag="VW343_002000"
                     /pseudo
     CDS             1769..1861
                     /locus_tag="VW343_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2183..2896)
                     /locus_tag="VW343_002001"
     CDS             complement(2183..2896)
                     /locus_tag="VW343_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756493.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(2889..3722)
                     /locus_tag="VW343_002002"
     CDS             complement(2889..3722)
                     /locus_tag="VW343_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756494.1"
                     /translation="MIEVSHLSKSFGDKIALNDISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQLLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSKFY
                     NISKLRVDNLLKRVGLYDSCKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSGLD
                     PTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKLVEQGAPSELIQR
                     YNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGKLN
                     A"
     gene            complement(3932..5080)
                     /locus_tag="VW343_002003"
     CDS             complement(3932..5080)
                     /locus_tag="VW343_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEU9756495.1"
                     /translation="MDSRYSRTKGYYSLFYNEEYNKIQNKTVLVLGAGALGCYISLSL
                     SMYGVRKLIVADYDIIEPSNLNRQILYTESDVGKEKINVLSQKIHKYNSDVQVVPISI
                     KVSSVEELENIVAEYGSIDFIVKAIDTPIDIIKIVNQFAVSHKISYISGGFNGCYLII
                     DNIYIPTIGSCFACRNINKDINKYTLSDKTKWPTTPEMPAILGGIMTNLIIKIFLGCY
                     NEILIDNAYVYNMRNHALSQEKYVLENGECPICKKNNKVKDNNIRAKTFIRSVCFCLL
                     SGGVAFLSAIGQFTVIETQLIVLFLGIIFAIYYAYYNKNIQTSLENIVWLFSSFEILF
                     LLVNFRTFIQLPVDIFISMIIFLMLWIFIMLGIVCLSYYITLLFSKEA"
     gene            complement(5117..5305)
                     /locus_tag="VW343_002004"
                     /pseudo
     CDS             complement(5117..5305)
                     /locus_tag="VW343_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
BASE COUNT         1812 a         1061 c          830 g         1794 t
ORIGIN      
        1 attttattta aaatattgtc atattttaaa taaaatggta aaagaggctc ggtagcctcg
       61 tctctcttta tcttactgac ctaaccttct tgccaatttt cttggtcgtc tttaaagcgt
      121 tttagaagat caaggccctc tggcgtgatg taaccttctt cttgggctag atggataagc
      181 tcgctatagt ttgaaagcgt cacaagttta acaccagcat ctgcaaagtt cttatctgct
      241 tttggcaatt ggtagctgaa aatcgctaca actccaagca catctgctcc ttctcgcttg
      301 gctgctgcta cagcttcaag aactgaacca cccgttgaaa taaggtcttc aaccactacc
      361 attttttgac cttgagctac gcgaccttcg atttgattac cagctccatg gtcttttggt
      421 ttgctacgga tgtaggcaaa aggcaagtcc atcttatcag caataatggc tccgtgtgga
      481 atccctgctg ttgcagttcc tgcaatcact tctacttcag gaaaggcttc tttgatagct
      541 tccacaaaac cattttcaat tagggtacga gtttctggat aggctagtgt cacacgatta
      601 tcagtgtaaa tcggtgactt gataccagat gcccaagtga agggttcctc tggtttgagg
      661 taaacggctt ggattttcaa gaggtggcta gcgatatctt tagcaagtgt catggtattc
      721 tccttttatt tttctaatct agttctttaa ttccagtcct gtgtccattc atccttgatg
      781 gcatgataag ctgcaacagg atcctcagct tgggtaatgg gacgtcccac tacgatatag
      841 tcactgccga tttgataggc atcagcaggt gtcatgactc gtttttgatc tccaactgca
      901 acaccagctg gacgaatccc tggtgtcaga cagataaaat ctggattggt agcctgcttg
      961 atgacttgta cttcctgagc cgagcaaaca acaccatcca agccagcttc agctgtcttc
     1021 ttggcatagt gaatcacaga ctcttgcaga ctggtttgga tattttgaaa ctcctgcatc
     1081 tgagcttctg acgttgatgt gagctgagtt acagcgatca atttggcttg acttccaaga
     1141 ccttcacgcg cagccttcat catctctaca ccaccagccg catgaacatt ggtcatatcc
     1201 acaccaagct gagataggat cttcattgct gacttgactg tattaggaat gtcatgaagt
     1261 ttgagatcca aaaagacact atgacccaaa ccttttaagt aggacacaat ctcaggcccc
     1321 gctgcgtaat aaagctccat ccctaccttg agataaaggc tttcttctgc tgggaaaaga
     1381 gctaaaaatt ccttgaccgc ctcaaaacta ggaaaatcaa gagcaatgat tggacgatgt
     1441 tctcgcatag gtttctcctt ttacctttac tagtgagaga gtctggccaa cagaagccac
     1501 gaaaaaagga acctgccaac agcaaggttc cacgaaaaat gggtagtctc caccctttca
     1561 ccgtctaacc ttagctgcct ctctggactg ctttaaaggt tttttactat tctattattt
     1621 ctactagcac ttgtcaagta aaaacatggt cacgtatgtg ttgactgcta ttgaacacct
     1681 cacaagactc tttccaaaag aatagttaaa gactttcact taggacagag aaaaagagtt
     1741 tgcttgttac cctttaattg aacaaatggg aattctgttt tattttgcgg atgcctattc
     1801 atcttggcaa aggggaagta atgagaatgc gaatggactt cttcgtgagt attttcttaa
     1861 accgatttgg caacaattac cgaaaaaatc cttaatggtg ctttgctagc ttattaatca
     1921 ccgtccccaa aaatacttgg attacaagac aggttttgaa gcacttatag aggagattta
     1981 aaaatgttgc attttttatt gcaatttatc agttatctta tcacccaaaa cttctgcaat
     2041 tagtatgagc actattgtta tttttctttt ttcgtacact caaatcaaaa tatagcaata
     2101 agactattct gtagaattgc tgtcaaaaag atcagttcat ttttccttcc cacttgtgtt
     2161 tataatcatt aaaataactc aattaagaaa ttttcttttt cctaatagtt atcgtaatta
     2221 aagttaatag aagaacaatc caaataagta gtgttagatt aggaattaga gtttcattcc
     2281 atgaaaatcc ctcccatttt gtgaaaaact tagtaaacag tcccataaaa ctataatctg
     2341 ttatcttcgc aactgtctta tccaaaccag atagcatcgg taagaaagca actaaaatca
     2401 tagcaggaag actgataacc tgagctacta cttggctctt cgcggttaaa cctatcaata
     2461 aatagaataa aatgatggat aaagaggtta gaagaacgat tgtaatataa ttaaaagtat
     2521 gtacaattgt aactcctaaa ataagaggag tagttcccat aatcacaaaa gttagcaaaa
     2581 aaggaagaaa catcgttgat aaaatgtatt cggatccttt aacaccactc aacagaagag
     2641 tttgtaaatt gtacttttct ttttcttcag acaagataat agttatagga cttccaacag
     2701 ccaaagccaa agaaaaaggt aaacacatca tcaaaagaac taatgcctgt tgatcgttga
     2761 ccttcccctg tgtttccata agatatttat aaaaatatgt gaaagcaaaa ggcactaaaa
     2821 cttgcaataa aatactctta ttggaaagga taatttgaca acgcaaccat accaaagcca
     2881 gaaaccgttt aagcatttag ctttcctcct gttaatgtga taaaaatatc ttctaaagta
     2941 ggctcacatg aatgaattga aaaaatattt tccagatcag cctgagatac ctgttctagt
     3001 gatgtaaaat caaaagttat ctgattccca ttataatctg taaccttaat ctttttatct
     3061 ttattatatc tttgaatgag ttcagaagga gctccttgct caactaattt ccctttattt
     3121 aataaggcaa cataatcaca taaaagagtt gcttcattca tatcatgagt cgttagaaaa
     3181 atcgttgtcc ctgctgtttt caattctaaa attaactcat gaattgttcg agaagttgtg
     3241 ggatctagac ctgaggtcgg ttcatccaga aagagtacag cgggtttgtt gataagagct
     3301 cgtgctaaaa gcattcgttg cctcattcca gtggataatt ttcctgctac catcttgcaa
     3361 ctatcatata atcctactcg ctttaacaaa ttatcaacac gtaatttact aatattataa
     3421 aatttactat aaaaaagaag attgttatac agagacattt tctcataaaa tccacttgta
     3481 tcgctaacca atccaattct ctttaattca ccgcttgtta aattttgaga tttttgtccc
     3541 aaaataatag attgtccttt atcggcaagg agctgcccag tcagaatatt aatcgttgtg
     3601 gtctttccag aaccagatgg tcctaaaaat ccaaaaatct gaccttcttt aacagtgaaa
     3661 cttatatcat ttaaagctat tttatcacca aaactttttg ataaatggct aacttcaatc
     3721 atactcattg cccctccttt atattctaca tttttactta acatctattt acttagcttt
     3781 atcaaataaa cgtcttaaaa aaaacaataa cattaagcat ataaccgtta gcaagattgt
     3841 ttcaatatta ttgataatat gatacattct caagcagtat aaaataatta tatatatgca
     3901 aatgctcatt atacctattt ttttaaacat actaagcctc cttgctaaat aataaagtta
     3961 tataataact aagacacact atacctaaca taataaatat ccacagcatt aaaaatatta
     4021 tcatactaat aaaaatatcc actggtaact gaataaatgt tctaaaatta acaagcaaaa
     4081 ataaaatctc aaacgatgaa aaaagccata ctatattttc caatgatgtt tgaatatttt
     4141 tattgtagta agcataatat atagcaaaaa taatacccaa gaacaatact attaattgtg
     4201 tttctataac agtgaactgc ccaatagcag ataaaaaagc tactcctcct gataataagc
     4261 aaaaacaaac tgaacgaata aatgttttcg ctctaatatt gttatctttc actttattat
     4321 tttttttaca aattggacat tctccgtttt ccagaacata tttttcttga cttagagcat
     4381 gatttctcat attataaacg taagcgttat ctattaggat ttcattataa catcccagaa
     4441 atattttaat tattaaatta gtcattatcc ctcccaaaat agcaggcatc tctggtgtag
     4501 tcggccactt tgtcttatca gataaagtgt acttatttat atctttgttt atattccgac
     4561 aagcaaagca agaaccgatg gtagggatat atatattatc aataataaga tagcatccat
     4621 taaaccctcc tgatatgtag gatatcttat gcgatacagc aaattgattg acaattttta
     4681 taatatcaat gggcgtatca attgctttaa cgataaaatc tatactccca tattccgcaa
     4741 caatgttttc taattcttct actgaagaaa ctttaataga aataggtact acctgaacat
     4801 ctgaattata cttgtgtatt ttttgagaaa gaacattaat cttctcctta ccaacatccg
     4861 actctgtata aagaatttgc ctatttaaat ttgatggttc tattatatcg taatcagcga
     4921 caataagttt cctcactcca tacatactta gacttagaga tatataacat cctaaggctc
     4981 cggctcctaa tactaatact gttttattct gtattttatt atactcttca ttataaaata
     5041 acgaatagta acctttagtt ctagaatatc tactatcaat atctatctca tccgaaatta
     5101 aaatttgttc atttaatagt tcaaatgtta gtttactacc taatacttct tctatctcta
     5161 atagttcctt acaactatta aatagatcgt tgataaattc ttgcgaaatt tgaggttcta
     5221 tagctatttc ccgcatactc cgaccacgca aatacattat accttctttt ttataagcaa
     5281 aaacataact atttacgtag aacatatttc gtccttcctc tcatatataa tatagaaagc
     5341 agtatagaaa caactacccc tcctatacta cgaacaataa tattaggtgt caaaaattgt
     5401 aatagttgac tcaaagggaa ataaataata acaaatgcta ttaaaagttg aactatatat
     5461 tgaaaattca agttattatt gttagaattc aagtatc
//
LOCUS       DAVKMU010000086         5455 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKMU010000086 DAVKMU010000000
VERSION     DAVKMU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.086"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            45..1799
                     /locus_tag="VW343_002005"
     CDS             45..1799
                     /locus_tag="VW343_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756496.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNINNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1783..3504
                     /locus_tag="VW343_002006"
     CDS             1783..3504
                     /locus_tag="VW343_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756497.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDVK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            3515..4099
                     /locus_tag="VW343_002007"
     CDS             3515..4099
                     /locus_tag="VW343_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9756498.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            4099..4794
                     /locus_tag="VW343_002008"
     CDS             4099..4794
                     /locus_tag="VW343_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9756499.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKIAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            4782..>5455
                     /locus_tag="VW343_002009"
     CDS             4782..>5455
                     /locus_tag="VW343_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756500.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRS"
BASE COUNT         1954 a          714 c         1024 g         1763 t
ORIGIN      
        1 cgaatttata tctaaaaggg atattaaaga aaggagatat gcttatgaag atttacaaaa
       61 aactatttgc ttatgtccaa gataagaaat atcttggggt tttggccata attttttctg
      121 ctatatctgc tctacttaca gtatatggat attatttaat ctacaaattt ctagataagt
      181 taataattaa ttcaaattta tccggtgcag agagtatagc attaaaatct gttattacac
      241 taacaagtgg agcgatattt tattttgtct caggaatgtt ttcacacatc ttgggattta
      301 ggcttgaaac aaatttaaga aaaaggggaa ttgatggctt agagaaagca agttttaggt
      361 tctttgactt aaatccatct ggtcaaataa gaaagatcat agatgataat gcagcacaaa
      421 ctcatcaggt ggtagcacac atgattcccg atagttctca ggcaataatc acacccgtac
      481 ttgtacttgc acttggcttt atagtaagta taagagttgg cataattttg cttgctctta
      541 ctataattgg cggcttaatt ttaggggcaa tgatgggcga gcaagaattt atgaagatat
      601 accaagaatc cctatctaaa ctaagtgctg aaactgttga gtacgtgaga ggaatgcaag
      661 ttgtaaaaat atttaaagca aatgtagagt cttttaaaag cttttataag gcgataaaag
      721 attactcaaa gtatgcttat gattattccc tatcttgtaa aaggccttat gttttgtatc
      781 aatggttatt ttttggactg attgcaattt taattattcc tatagtttat tttatgacta
      841 gcttagctag cgcaaaggtg attttacttg agcttatcat gattttattt ttatcaggag
      901 ttctctttgt ttcattcatg agaatgatgt ggtactccat gtatatttct caaggaaatt
      961 atgcagtaga tactttagag gcgctttacg aagatatgca aaaagacaaa ttagtgcatg
     1021 gtaatatcaa taattttaaa aactataata tagaatttga gaatgttagc tttgcttata
     1081 atgataaagc tgtcattgaa aatttatcct ttaatttaga agaaggaaag tcctacgcac
     1141 ttgtcggttc atctggatca ggcaaatcaa cagtagcaaa acttatatca ggtttttata
     1201 atgttaataa aggaagcata aagataggcg ggatagcaat aagtgaatat tctgacgaag
     1261 ccttaattaa agccatttcc tttgtttttc aagattcaaa attattcaag aagagcattt
     1321 atgataatgt agctttagct aataaagatg cgacgaaaga tgacgttatg agagccttaa
     1381 aattagcagg atgcgattta atattagaca aattcccaga aagagaaaat acaatcatag
     1441 gctcaaaagg tgtttattta tccggtggag aaaaacaaag aattgcaatt gctagagcaa
     1501 ttttaaagga ttccaaaatt attattatgg atgaagcatc agcatctatt gacccagata
     1561 acgagtttga attgcaaaaa gcttttaaaa atcttatgaa ggataaaaca gttatcatga
     1621 ttgcacacag gctatctaca attaaagacc ttgatgaaat tattgtcatg gatagtggaa
     1681 aaattataga aagagggtct gacaaagaat taatgtcaaa agatacaagg tataagagcc
     1741 tgcaagagat gtttaacagt gcgaatgaat ggagggtttc aaatgaaaga gttttataaa
     1801 aaaagatttg ctcttacaga tggaggagca agaaatttaa gtaaagcaac actggcttca
     1861 tttttcgttt attgtataaa catgcttcct gccatattac ttatgatttt tgctcaggaa
     1921 gttttggaaa atatgggcaa aagcaatggc ttttatatag tattctcagt tttgactttg
     1981 atagcaatgt atattttgct ttctatcgaa tacgataaat tatataacac aacctatcaa
     2041 gaaagtgcag atttaagaat aaggacagcg gagaatttat caaaattacc tctatcttac
     2101 ttttctaaac atgacatttc cgacatttca caaacaatca tggctgatat tgaaggcata
     2161 gagcatgcaa tgagccactc aataccaaag gtgggcggca tggtactgtt tttcccatta
     2221 atatctgtaa tgatgctagc gggcaatgtc aagatgggtt tagctgtaat tattccatct
     2281 attttaagct ttatatttat acctttatct aaaaaatatc aggttaatgg acagaataga
     2341 tattatgatg tcttaagaaa aaactcagaa agctttcaag aaaatatcga aatgcaaatg
     2401 gagattaaag catataattt atcgaaggat attaaagatg acttatataa aaaaatggaa
     2461 gatagtgaga gagtacactt aaaggcggaa gtaactacaa ttttaacttt gtctatatct
     2521 tcaatattta gctttatatc tcttgctgtt gtgatatttg tcggcgtaaa tctaattatt
     2581 aataaagaga taaattctct ctaccttata ggatatttac tagctgctat gaagataaaa
     2641 gactctttag atgcatctaa agagggcttg atggaaatat tttatttatc gcccaaaata
     2701 gaaagattaa aagaaattca aaatcaagat ttacaagaag gcgatgacta tagcttaaaa
     2761 aaatttgata ttgatctaaa agatgttgag tttgcctaca ataaagacgc aaaagtttta
     2821 aatggtgtaa gttttaaagc taagcaggga gaggtcactg ctttggtagg tgcaagtggc
     2881 tgcggtaaaa caactatctt gaaacttata tcaagacttt atgattatga caagggacaa
     2941 atcttaatcg atgtcaaaga tataaaagaa atatcaacag aatctctttt tgataaggtc
     3001 tctatagttt tccaagatgt ggttctcttt aatcaaagcg ttatggaaaa tattagaatc
     3061 ggaaagcaag atgcaagtga cgaagaggtt aaaagagcag caaaacttgc aaattgcaca
     3121 gattttatag aaaaaatgga taaaggtttc gatacagtta ttggtgagaa cggagctgag
     3181 ctatcaggag gagaaagaca aagattatca atagccagag ccttcttaaa agatgcgccg
     3241 atattgatct tagatgagat aacagcaagc cttgatgtta acaacgagaa aaagattcaa
     3301 gagtctttaa ataatttagt taaagataaa actgttgtaa tcatttcaca tagaatgaaa
     3361 tccatagaaa atgcagacaa gatagtagtt cttcaaaacg gaagagtaga aagcgaaggt
     3421 aagcatgaag agcttttaca aaaatcaaaa atttacaaaa atttaataga aaagacaaaa
     3481 atggcagaag aatttattta ttaggaggac tacaatggat aataaaaaat taaaagtaaa
     3541 agatttagta agtatcggtg tttttggcgt aatttatttt gccttcatgt ttggagttgg
     3601 tatgatgggc ttgattccaa tattgttctt aatatacccg acagtattag ccatagttgc
     3661 aggaactgtt gttatgttat ttatggctaa ggttcaaaag ccatgggcac tatttatatt
     3721 tggtatgata tcaccacttg tgatgtttgc agctggtcat acctacgtag ttgtggtttt
     3781 atcacttata gtaatgataa tagcagaatt aattagaaag attggtaatt ataattcatt
     3841 taaatacaat atgctttctt atgcaatctt cagcacatgg atatgtagct ctttaatgca
     3901 aatgctttta gcaaaagaaa aatatatgga gtggtctttg atgacgatgg gaaaagatta
     3961 tgttgatgta ttagaaaagt taataactta tcctcacatg gctttagtag ccttaggtgc
     4021 tttcttagga ggaattcttg gagcatatat aggcaaggct ctattgaaaa aacactttga
     4081 aaaagcaggc atcgtataat gcctactttt tcttcaaatg ctccctttaa tcccaatccg
     4141 atctctaaga tattagtcgt atttatcaca ggcttaactg ttatgcatag cataaacatc
     4201 cgttttgagc tggcaattgt ttgtcttatt ggtattttgc tttatttgaa tggatacaaa
     4261 aaaacacttt tcaaatggat tatattgtgt gggatacttt actccttgcc taattttatg
     4321 gtgttatctg aattaaaccc tatagttaag atgtttttga gtatacctat tgttattaga
     4381 atgtttattt taccatttat ggcagcaagc tttatgataa agacctcgga tgtaggcgca
     4441 ataatttcat cgatggataa gcttaagatt tcaaagaatg tatccatacc tattgcggtt
     4501 atgtttagat tcttcccatc ttttaaggag gagaagaaaa acatcaaaat agctatgaga
     4561 gtaagaggga taaattttaa aaacccagtc aaatatcttg aatatgtttc tgtgccacta
     4621 ctcattatat catctaatat atcagatgac attgcaaaag cggcagaaac aaaggcaata
     4681 gaaaatccaa ttgccaagac cagatacatt cgcgtaaaga tacagctaat tgattttgtt
     4741 tatgttttag cggttgctgg acttattgtg ggaggcttaa tatggttgaa ataaaaaatt
     4801 taagtcttga ttatggtgaa gagcatatat tagatgatat atcactatcc atagccgagg
     4861 gagagtgcgt gctatttaca ggaaaaagtg gaagtggtaa gtcatcttta ataaattcaa
     4921 tcaatggact agctgtaagg tatgataacg caaagacaaa gggcgaaata attattgatg
     4981 gtaagaatat aaaaaatttg gaactttatc aaatctcaat gcttgtttca actgtttttc
     5041 aaaatcctaa gacatatttt tttaatgtca atacgacatt agaattatta ttttatttgg
     5101 aaaatatcgg tcttgcaaga gaagagatgg acaggcgttt gaaggatatg cttgagatat
     5161 tcccgataaa aaatcttttg aacagaaata tatttaatct atccggcggt gaaaaacaaa
     5221 ttctttgcat tgcagcttct tatatagcag gtacaaagat tatagttatg gatgagtctt
     5281 catcgaattt agatattaaa agcataagtg ttttggcaaa gatgctaaag atattaaaag
     5341 agaaaggcat aagcataatt gttgcagagc atagaattta ttatttgatg gacatagttg
     5401 accgtgtatt tttaatagat aaaggaaagc ttaaaaaaac ttatactaga agtga
//
LOCUS       DAVKMU010000087         5387 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKMU010000087 DAVKMU010000000
VERSION     DAVKMU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.087"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..782
                     /locus_tag="VW343_002010"
     CDS             <1..782
                     /locus_tag="VW343_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9756501.1"
                     /translation="IEDVFNTKSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYVLK
                     GINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKI
                     FGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEG
                     NNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHR
                     LDTIKSADKILVMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            806..2544
                     /locus_tag="VW343_002011"
                     /pseudo
     CDS             806..2544
                     /locus_tag="VW343_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            2595..3362
                     /locus_tag="VW343_002012"
     CDS             2595..3362
                     /locus_tag="VW343_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9756502.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            3364..3996
                     /locus_tag="VW343_002013"
     CDS             3364..3996
                     /locus_tag="VW343_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756503.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(4942..5355)
                     /gene="ndk"
                     /locus_tag="VW343_002014"
     CDS             complement(4942..5355)
                     /gene="ndk"
                     /locus_tag="VW343_002014"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9756504.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
BASE COUNT         1874 a          798 c         1015 g         1700 t
ORIGIN      
        1 gaatagagga tgtgtttaat actaaatccg aattaattcc agaaagagtc agtcaagata
       61 taaaatttga taaaaaaata gaattaaaag atatttggta taaatatgga ttatttgatg
      121 attatgtttt gaaaggaata aatgttacta ttaaaaaagg agaaactgtt gctattgttg
      181 gagaatcagg ttcaggtaag agtacattag ctaaaatttt attaggttta ttagaaccta
      241 atattggttc aatagaagtt gatggagtag aaaaagaaga aattggtcaa acattgtata
      301 gaaagatttt tggagcagtg ttacaaaatt caaccctaag ttatggtacc ttaagagaga
      361 atttgacatt tggacacttt gtttcagatg aagaattaat gacaaatcta aattcaattg
      421 gtcttagcaa tgtagttaaa tctttacctc ttggattaga gacaatcatc gctgaagaag
      481 gtaataactt ttctggaggg cagcagcaaa tgatactttt agctcgttgt cttttgtcga
      541 aaccttcggt agttgttttg gacgaagcaa caagtagttt agataattta tctcaacaaa
      601 ttacaacttc ttacttaagt gaaatcggta ccactaagat tttaattgcc catcgactag
      661 atactatcaa gtctgcagat aagatcttag taatgcataa tggtgaaatt gtagagattg
      721 ggacccatag agaacttctt gaactaggag gcatttataa gcaattgtat tcaaataatt
      781 agtttttgat taaaagggta aatttatgaa gattatgaaa aaaaaatatt ggactttagc
      841 gatattattc ttttgtttgt tcaataattc tgttactgct caagaaatac ctaaaaatct
      901 tgatggcaat ataactcaca ctcagactag cgaaagtttt tctgaatctg atgaaaaaca
      961 ggttgactat tctaataaaa atcaagaaga agtagaccaa aataaatttc gtattcaaat
     1021 cgataagaca gaattatttg taacaacaga taaacattta gaaaaaaact gttgtaaatt
     1081 ggaacttgaa ccacaaataa ataacgatat tgttaactct gaaagtaata atttactagg
     1141 cgaagataat ttagataata aaattaagga aaatgtttct catctagata atagaggagg
     1201 aaatatagag catgacaaag ataacttaga atcgtcgatt gtaagaaaat atgaatggga
     1261 tatagataaa gttactggtg gaggcgaaag ttataaatta tattctaaaa gtaattctaa
     1321 agtttcaatt gctattttag attcaggagt cgatttacaa aatactggat tactgaaaaa
     1381 tctttcaaat cactcaaaaa actatgtccc caataaagga tatttaggaa aagaggaggg
     1441 agaggaagga ataatatcag atattcaaga tagattaggt catggtacgg ctgttgtagc
     1501 tcaaattgta ggggatgaca atattaatgg agtaaatcct cacgttaata ttaacgtcta
     1561 tagaatattt ggtaagtcgt cagctagtcc agattggatt gtaaaagcaa tttttgatgc
     1621 tgtagatgat ggcaatgata ttatcaatct tagtactgga caatatttaa tgattgatgg
     1681 agaatatgag gacggaacaa atgattttga aacatttttg aagtataaaa aggctattga
     1741 ttacgcgaat caaaaaggag taattatagt agctgcatta gggaatgact ccctaaatgt
     1801 atcaaatcag tcagatttat tgaaacttat tagttcacgc aaaaaagtaa gaaaaccagg
     1861 attagtagtt gatgttccaa gttatttctc atctacaatt tcggtcggag gcatagatcg
     1921 cttaggtaat ttatcagatt ttagcaataa aggggattct gatgcaatat atgcgcctgc
     1981 aggctcaaca ttatctcttt cagaattagg acttaataac tttattaatg cagaaaaata
     2041 taaagaagat tggatttttt cggcaacact aggaggatat acgtatcttt atggaaactc
     2101 atttgctgct cctaaagttt ctggtgcgat tgcaatgatt attgataaat acaaattaaa
     2161 agatcagccc tataattata tgtttgtaaa aaaattctgg aagaaacatt accagtaaaa
     2221 aatggtataa aagtgttaaa tataccaaac gtattgagat atgatttgaa tatgttacaa
     2281 ttagaatata aaaatgaaca aagttgggat agtttcatag ataatgttaa tttaattgag
     2341 ttggaagaga gaattcaaac tactattgga attaaacaaa taaacacaca caatattatt
     2401 actattgccc gagaagggta ctctcaaaat tatttaccta acacttcaga aaatacatat
     2461 aattcattac aagtcagttt agttggagta ttactacttt ttataagtat ggtaaatatt
     2521 ttatgggcta aaaaaagtaa atgaaaataa aatttggagc cctctgaaaa agtaagtcct
     2581 acagttcaac taaaatgagt caaaagatga atcaccttga tgtaggggag tttgtcttat
     2641 tgctgcctga acacctccgt tcagaggaag aacattataa atctgttttt gaagacgact
     2701 taaccagtcg catatctagt caagatgaac gacagcaaat gactgctacg gtaggttatt
     2761 tagaatcagg tcaggatcgt tttgtgtata atacgacccc tatttcttac cagcagtttt
     2821 tgaaagatcc aatcatcatt gttataacac cccaatcaac tggtccacag tccattttgt
     2881 tttggataga cgcagtacag aactacgttc tctttaatca attgtctgat gcccaggagc
     2941 ttatccagag acaaggcatt gaaaattggg tctcagaaat gcaaacaggt taccacaact
     3001 acatcacatt attggataat atccagaggg aacgttgggt aatgctagca ggagctgtgc
     3061 ttgggattgc aacttcaatc ttgttgttta acactatgaa taggctctac tttgaagaat
     3121 ttagacgtgc catttttatc aaacgcattg caggtctcag gttcttagaa atccatcgca
     3181 cttatctctt tgctcaactg ggtgtgtttt tactgggatt tgttgcgagt gtatttcttc
     3241 aggtagagat aggagttgct ttcttagtct tgttactctt tactggtcta tctcttttac
     3301 agttacatgt ccaaatgcag aaagaaaaca agatgtccat gcttgttttg aagggaggtt
     3361 aatatgattg aacttaaaca ggtgagtaaa tcttttggag aacgagagtt attttcgaat
     3421 ctttcaatga catttgaggc tggaaaagtc tatgccttaa ttggttcaag tggtagcgga
     3481 aaaacaacct tgatgaacat gattgggaaa ttagaacctt atgatgggac gattttttac
     3541 cgaggtaaag acttggccaa ttataaatca agtgattttt tccgtcacga attgggctac
     3601 ctcttccaga actttggctt aattgaaaac caaagtattg aagaaaacct taagctaggt
     3661 ctcattggtc aaaagttgag tcggtcggaa cagcggttga ggcagaagca ggctttagaa
     3721 caggtcggcc tggtttatct tgacctagat aagcgcatct ttgagttatc gggcggagaa
     3781 tcgcaacggg ttgccttggc aaaaattatc ttaaagaatc caccctttat tctggcagat
     3841 gagccaacag cttcaataga cccagcaacc tctcagttga ttatggagat tttgctatct
     3901 cttcgagatg ataataggct aatcattatc gcaacacata atccggcaat ttgggagatg
     3961 gctgatgaag tgttcacgat ggatcatctg aaataaaaat ccttgttttt aattgcacga
     4021 tgagttactg aaatattatc atgaatcaag aattggagtt aatttagaat tgtacttaat
     4081 ttagaattgt actttattaa tattgaggta actttttctt gataaaggaa gaaataatgg
     4141 agaggaagtt agaatgaaaa aattcgacaa ttatattatt gagaagcctt gcgattctaa
     4201 ttcagataaa ctgcaaaaaa tcttaataat tgaaagtttg gtagatgata ttttgcaatt
     4261 ttctctcaga atcaataata gtgtaggaga gattttcctc ctacaaccgt tttaaaagaa
     4321 aactatcttt attccatgtt attttgagga agatattgtg aaagtcaaag atgatgataa
     4381 agttgagtgg aatttgttag aatttcaaaa atttagagca tttttggctt agtaatctgt
     4441 gttgaaggct caaaacctat ggtaaaaaag tagctttgaa aacgtattgc ctccaaagat
     4501 ttagttaaat aatgatttaa cacaaaaaga aattattgaa gttctggaaa gatgttgttt
     4561 cagtattgag aaaaggtggg aaaaacttgc gattttcaca gagaaaggaa gaaaaagtat
     4621 agaaatatag tcaattgaaa caagaacagg ataaaagaac cttttgtgcc atatttttct
     4681 cctttcgctt tacaattgga ttgaacacct ttattgtatc gcgtatggag tttttttggt
     4741 ataaccttcg acgcacaccc gcatagcggg tgtttttttt gtctcgcacc taacggagcg
     4801 agacaaacta atagtcactt aatcaaaaaa cgcaccatat caaaaactaa aaagtttgat
     4861 atcatgcgtc atgtcttaaa ctaattgact atactttcta ttcaaatgag cttttaacca
     4921 attgattgag ccaatccact cttaaaacca aagagcaatt tctcgcttag ctgactcttc
     4981 tgaatctgaa ccatgtacaa cattttggat aatctcattt tctccagcag cttttgcaaa
     5041 atcacctcga atagtgcctg gtaaagcttc ttctggacga gttgcaccca tcatggtccg
     5101 ccaagtttcg attactttgg gaccagaaat gacacccaca agaactggac ctgaagtcat
     5161 gaattcacga atcggtgggt aaaaactctg accaaccaag tcctgatagt gctggtcaat
     5221 caactcttct gaaacctgtg aacgaaactc caatttttcg attgtaaatc cacgttgttc
     5281 gatgcgcttt aacacttcac ccactagccc tctttttaca ccatctggtt tgatgataaa
     5341 gaatgtttgt tccatacccg tctcctttgt cagcttcttt cttttat
//
LOCUS       DAVKMU010000088         4421 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKMU010000088 DAVKMU010000000
VERSION     DAVKMU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.088"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            93..185
                     /locus_tag="VW343_002015"
                     /pseudo
     CDS             93..185
                     /locus_tag="VW343_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(611..808)
                     /locus_tag="VW343_002016"
     CDS             complement(611..808)
                     /locus_tag="VW343_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9756505.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
     gene            complement(793..1071)
                     /locus_tag="VW343_002017"
     CDS             complement(793..1071)
                     /locus_tag="VW343_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9756506.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(1110..1895)
                     /locus_tag="VW343_002018"
     CDS             complement(1110..1895)
                     /locus_tag="VW343_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9756507.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(1897..2715)
                     /locus_tag="VW343_002019"
     CDS             complement(1897..2715)
                     /locus_tag="VW343_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9756508.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(2717..3709)
                     /locus_tag="VW343_002020"
     CDS             complement(2717..3709)
                     /locus_tag="VW343_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9756509.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1491 a          931 c          758 g         1241 t
ORIGIN      
        1 ttgttatctc tctataaaaa cagtatatca tttaaaaaaa tttaagtcaa aaaaattaac
       61 attagttaat ttatttttta gcacacatta aaaaataaga ttagtactca atgaaaatca
      121 aagagcaaac taggaaacta gccgcagatt gctcaaaaca gtgttttgag gttgtagatg
      181 gaactgacgt agtcagctca aaacactgtt ttgaggttgt ggatagaact gacgaagtca
      241 gtaaccatac gcacggcaag gcgacgttga cgtggtttga agagattttc gaagagtata
      301 agtccaaaac tttcgttaac cgggatacat tataagcaca ttcataccat caaaaatagt
      361 agcaataaac ttgaacaact accagaaaca cgaaaacagg cataattgat aaaatattag
      421 cgctattaaa caatataaat aaccgctaag agtaaggctt aacggttatc ttctacctat
      481 ctattttttc aaatcctcag tcgtttatct acctataacc taggctttac gagtgccttg
      541 ctctaccaac taagctaaat cggcgattac acttacagta tagcactctg agaatagatg
      601 tcaagtaata ttattcaagc tttttaatca aaatctgaca aggattttgc ggattccata
      661 gttgaggaaa aatctctaac tttttaaagc caagacctcg ataaaagtca tttgttcgat
      721 cataatcttt attagaacct tctgccactg ttttgacctg cagataacca acttttttac
      781 gagcttcact ctctaaagta gcaagcaatt ggctcccaat ttttctacct tgataagctt
      841 ttttacgccg agacaatcaa tctctgcaca atcttcactc gaataggata agcttacaaa
      901 tctagtcaaa tcactctcct gataggcggt ccaaacttgc agtgtcgtgg ttccttctat
      961 ataggcttgt gtgctttctg ggattccaaa ccattctggc aaatccttca aaacctcagc
     1021 gacaactgcc tttttttgat tttcatcctt gacttcttca attacaaaca tcataagttt
     1081 ctttcttgat tttagtctta ctttagcttt tacgaacccg cactttcgta ggaatctaag
     1141 cccttatccc aaagtttaag ggaaataaca aagaaaacca gagaaatcaa catcaaacct
     1201 cctacgttaa agaacacatc cttttcctgt aagaaatagc tagctggata gtaggctgta
     1261 aaggcgaaag gcacgataaa gctaatcaac caacgaagaa gagaattgta aatagaaatc
     1321 ggatacttag caaagtcatt gaacatatag aagatgtaaa tcatggcgcc tgactgctta
     1381 gtccaaaagg cgatactggc tgttgcgatt ttaagagaag tataaatcaa ggtcgcaaaa
     1441 ggaatacaaa ctaggaaaag caggaatttt ggaagagtcc aaacaatgct ggtcactgtt
     1501 gttcccaata aaataccacc gactaagagt tcacccaagg catcaatctg aaaggtttca
     1561 actaggatgt gaaagagagg attgatggga cgagtcagat acttgtcaaa ctcccctttt
     1621 cggactaggc gttgccctag tgcccagaga ttgtcaaaaa agagatggtc cattcccttg
     1681 ggaatcaagg aaaatccata aatgaaagct atctcttgaa aggtccagcc ttctaggaat
     1741 ggaatatgtt gaaagatgac attgagaaac aagagattca agccttgagt cagaaagact
     1801 cccaagacac caaccacaaa atctacctta tattccatga tttgtttgat gtattgtctg
     1861 ataaaaatca gatgcattcg ttgatatttt ttcatactaa cctccttgaa tggtgataaa
     1921 ggactggacc cgtttccaaa ttaactgaga caatcccacc atcactaaga gccagaagaa
     1981 ctgcaaaagg agtgcctgaa gaatctgact ggcatcgtat tttccaacaa tgatcataac
     2041 tggagtataa atcaaggatg aaaaaggcaa aaaggagaga atatctgaaa caacctttgg
     2101 aaaaaatgcc aagggaatca aactccccga cataaaagcc actatggaag tcttaagtag
     2161 gttggaaccc caaagatttt taaacacaaa ggctgaaaat ccaaagcaaa tattaaagaa
     2221 aaagttaatc agataggcga gcgttaagct aaaaagataa atgacagtta atcctagcac
     2281 ctctacaata ccttgacccg atatgatttt catcaagaca atgacactta aaaatggaag
     2341 gccaacgctg ataaaaatca accacttgga accaagctcg gtgaaaagat aggaggccgc
     2401 aaaatgcact ggtcgcaaca aacgcatgat aatggagcca tccttgacct cctccccaat
     2461 cataaaggac gaatcggatc tagtcagaag attggtcaca aaactcatga tgatgtagag
     2521 ggtgatatcc gccatactga agccctgaat caaagactct tgcgaagaat caaagacagc
     2581 cttccagaga taaaaggcca caaaagcccc catgacatcg ccaatccgat agagaataaa
     2641 gttgactcgg taagtaatca actcctgaac ccctgcattg ataaagggtt tataacgtct
     2701 ccataatttg atcatcctag agctcctttc ggtagaagcg acggataata tcctcaatat
     2761 ccgtatccac catcttcaaa tcgcggattt caaaatcaga cagggtttgc ttgataatgt
     2821 cagctgactg gtagcgagaa ctatcaaatt caatgttgag gctgtttcct tgtctatcaa
     2881 tggtcatatc agacagacct tcatagtgag agacgagatg actttgacct ggtagcagtt
     2941 caaaagagag agtcttcatc ttaccaaagg tctccttgag ttggctcacc gttccatcaa
     3001 aaatctcttg ccccttgtca atcatgaaaa tccgatcaca aagttgctca atatcactca
     3061 aatcgtgagt ggtcaaaaga atggtagttt cttcctcttg attgatctga gtaattgccc
     3121 gacgaatatt atccttaacc gaaacgtcca aaccaatggt cggctcatct aaaaaaagaa
     3181 ccttgggatt gtggagcaag gaggccgcaa tatccgcccg catccgttgt cccagtgaaa
     3241 gagtccgcac gggatccttg ataaagtcct tcaaatccaa gacttcattc aaaaagtcca
     3301 tgcgcttatg aaagagcgag tttggcacat cataaatctc ttttaagaca gtgtaggtct
     3361 cttgcagagc caaatcccac catagctggg tgcgttgtcc aaagactacg ccaatatctt
     3421 tgacataatc ttgccgattg tcctggggaa tcttgccgtt aatccgacaa aaaccagatg
     3481 ttggtttcaa aattcctgtc agcattttaa tggttgtcga cttcccagca ccatttgccc
     3541 cgataaatcc taaaatctgc ccttttggaa cctcaaaggt caaatccttg accgcttcaa
     3601 aggtctgctt ttcaggatga ataaaggagc gcaaagcccc cttcaagccc ggttccttaa
     3661 cagtcttcac aaaatttttc tgaagatgtt ccacttctat cattgccata tctatctccc
     3721 tatcgtaagg aatagcaaca ttgtgttttt aactacaaat attctaaatc cttgctttct
     3781 acaccttcta cattttatta ctacaaaagg ctttatacca acctattata gtccaataaa
     3841 aaacgaaatg caagcgtttg cctatggatt gtgaaattag gatttctctc ataaaataat
     3901 acttttaatc aaaaattctg tttaatagtt taaattacca acctactcat cttctcacca
     3961 gagggcggta ttaactttga tcataaaatc ggatactccc tctatccaga aaatatgctc
     4021 agaatgattg tctctaaaca atacctgctc ttttacctca ttcatgaaaa agaagtccat
     4081 accctgagaa ttatcaactc acgcatcgac tacctaaacc aactcgacca cctctttcgt
     4141 acatgtagga aactattttc ctcacaaata atctcattat aaaatccaat aaattttttt
     4201 agctcacttg aatattttct ttgtatcgtt ttatcagctt caatctgatc tagacttgtt
     4261 accttggaac gacttgaaat aacctttata aaataacact taaggctttc atcatctcgt
     4321 ataaattttt caatagtgat ataaaaagct ttcatattct gtttgtaatc agatatcaat
     4381 tgattaattt tatcattctg atgaagattc gataccataa t
//
LOCUS       DAVKMU010000089         4323 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKMU010000089 DAVKMU010000000
VERSION     DAVKMU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.089"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            27..1052
                     /locus_tag="VW343_002021"
     CDS             27..1052
                     /locus_tag="VW343_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9756510.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1042..2049
                     /locus_tag="VW343_002022"
     CDS             1042..2049
                     /locus_tag="VW343_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9756511.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2049..3056
                     /locus_tag="VW343_002023"
     CDS             2049..3056
                     /locus_tag="VW343_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9756512.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFVLLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3056..3850
                     /locus_tag="VW343_002024"
     CDS             3056..3850
                     /locus_tag="VW343_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756513.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT         1564 a          634 c          737 g         1388 t
ORIGIN      
        1 acttgaaata tgtttaagga gtttttatga aaaacaaatt ttttctaata gctattttag
       61 ctatgtgtat agtttttagc gcttgttctt ctaattctgt taaaaatgaa gaaaatactt
      121 ctaaagagca tgcgcctgat aaaatagttt tagatcatgc tttcggtcaa actatattag
      181 ataaaaaacc tgaaagagtt gcaactattg cttggggaaa tcatgatgta gcattagctt
      241 taggaatagt tcctgttgga ttttcaaaag caaattacgg tgtaagtgct gataaaggag
      301 ttttaccatg gacagaagaa aaaatcaaag aactaaatgg taaagctaac ctatttgacg
      361 atttggatgg acttaacttt gaagcaatat caaattctaa accagatgtt atcttagcag
      421 gttattctgg tataactaaa gaagattatg acactctatc aaaaattgct cctgtagcag
      481 catacaaatc taaaccttgg caaactttat ggagagatat gattaaaatt gattcaaaag
      541 ccttaggtat ggaaaaagaa ggtgatgagt taatcaaaaa tactgaagct cgtatatcca
      601 aagaattaga aaaacatcca gaaatcaaag gaaaaatcaa aggaaaaaaa gtattattta
      661 ctatgattaa tgctgcagat acatcaaaat tctggattta tactagcaaa gatccaagag
      721 caaattattt aacagattta ggtctagttt tccctgaatc attaaaagaa tttgagagtg
      781 aagatagttt tgcaaaggaa atttctgcag aagaagcaaa taagataaat gatgctgatg
      841 taatcataac ttatggtgat gataaaactc ttgaagcttt acaaaaagat cctcttttag
      901 gtaaaataaa tgcaattaaa aatggtgccg ttgctgtaat tccagataat acaccgttag
      961 cagcctcatg cactccaaca ccactttcaa taaactatac tattgaagaa tacctaaatc
     1021 ttttaggaaa tgcatgcaaa aatgcgaaat aaaaaacaaa taaacctagg cataattttt
     1081 gtaatctgcc taggtcttct tattacaata tttttgtcat taaagcttgg aacaaaagaa
     1141 attaatatca gagatttttt agcagctttt ggaatgggta atacaaatga tgattttatt
     1201 aaatcaatta tatataaaag aatacctaga actatttttg caattttagc aggttctagt
     1261 cttgccataa gcggtgtatt gatgcaatca gttactagaa acccaatagc tgatccaggt
     1321 atactcggta taaacacagg agcaagtctt agtgtagtaa ttggtctttc ttttttagga
     1381 atttcatcaa gcataagcca tataagtttt gcaatcattg gtggcttagt aagtgcaatt
     1441 tttgtatacg cgattgctgt aagcggaaaa gcaggcctta cccctataaa acttgcctta
     1501 tcaggaactt gtgttagtat ggctttaagc agttttgtaa gttttttaat tttaccgaat
     1561 aataacgtct tagacaaatt tagattttgg caaataggta gccttggagc agctacatta
     1621 tcttctatat ctacactact accttttata attttaggtc acttgatagc tatatttatt
     1681 tcatcagatt taaacgcttt agctatgggt gatgaaatgg ctgttggtct tggagttaat
     1741 gttaatagga taagatcact tgcaataatt gcaagtgtgc ttttatgttc aagtattact
     1801 gcaattggtg gacctattgg cttcgtaggt cttatagttc ctcacttttg tggcttattt
     1861 ataagcaaag atatacgcac aatgaccatt tcttcatctt ttataggtgc agagctcttg
     1921 cttatatgtg atataatcgg ccgtatgtta ggtaaaccag gtgaaattga agtagggata
     1981 attactgcaa taatcggggg tccagtactt atttatgtaa ctatgaaaaa tagaggggtt
     2041 aataactaat gcaaaattta attataggta ttcaaaaaag aaaaaataga ataacactat
     2101 tttcctcact atttttatta ataataatca gtctatcatt tttcgtttta cttatcggag
     2161 atgaaagtta ttctttttca actttgatta aagtcttaaa tagtgaaact gttcctggag
     2221 ctagtttttc gattatggaa attagattac caaaattatt agcaggaatt atagctggct
     2281 ggtcttttgg attggcagga tttatctttc aaactatgtt aagaaatcct cttgcaagtc
     2341 ctgatataat cggtgtcaca agttcttcat ctattgcagc ggtcttttgc atattggtat
     2401 taaaaacaaa tagtttaact actggaatta tttcaataac ttgtggacta acatcatctt
     2461 taatattatt tttactagct aaaaaagatg gtttttcagc agcaagactg ataatattag
     2521 gcattggttt tcaagctgtc acaagagcag gcacctcatt tttattgttg aaagtagcaa
     2581 gatatgaatt acaagaagtt atgagatggc tcagtggctc tttatctttt acaaagttag
     2641 atgacatacc tcttgttcta atagtaagta ttattgctac tatattagtt ttatttttta
     2701 ataaaagact agaaattatt gaacttggtg aagaaatagc aatcggactt ggagcaaatc
     2761 ccgagctttc aaggcttgtt ttaatttttt gcgctgtatc tttaactgct ttttctactt
     2821 caattacagg accaatagct tgtatatctt ttttagctgg tcccatagcc ttaaatattg
     2881 gcaagaaaag aagcccaata ttagctggat tggttggaat tttactagtt ttgttatcag
     2941 acatattctc tcaaaatatt ttaccagcta gatatccagt aggtgttgta actggcttgt
     3001 taggttcacc atacttaata tatttactaa taaaaatgaa caggaggaat atataatgaa
     3061 aggtctgtgg tcaaataatc ttacctgcgg ttatgatgaa aaaataatct tggaaaatat
     3121 aaatataaaa atacctgaag aaaaaatatc agttattatt gggtcaaatg gttgtgggaa
     3181 atcaacactc attaaaacct tgtctcgact tataaagcca ttagagggag aagtattgct
     3241 tgataataaa tcaattaatt cttataaaga aaaagattta gcaaaacaca tagctatatt
     3301 acctcaatct ccaataatcc ctgaatcaat aacagtagct gatcttgtaa gccgtggtcg
     3361 tttcccctac agaaagcctt ttaagagtct tggaaaagat gaccttgaaa taataaacag
     3421 atcaatggtt aaggccaatg ttgaagatct agcaaataac ctagttgaag aactttctgg
     3481 gggtcaaagg caaagagtat ggatagctct agccctagcc caagatacaa gtatcctact
     3541 tttagatgag ccaactactt acttggatat ctcatatcaa atagaactat tagacctctt
     3601 gactgatcta aaccaaaaat ataagacaac catttgcatg attttgcacg atataaatct
     3661 aacagcaaga tacgctgatt acctatttgc aattaaagaa ggtaaacttg ttgcagaggg
     3721 aaagcctgaa gatatactaa atgataaact agttaaagat atctttaatc ttgaagcaaa
     3781 aattatacgt gaccctattt ccaattcgcc tctaatgatt cctattggca agcaccatgt
     3841 tagctcttaa taattgaata ttggaattca tttaaaagtc gcaaattttg aactgaccct
     3901 caaaagttag gccaaaaaac taactaaagg aggtcagttt tttaatggca aatacagcac
     3961 agaatttaag ataaaattag taaaagaata tcatgaatga aaaataggat atagagaata
     4021 acagcaatat taaaaagatc aggactgata ataaacaaaa agaaagtaca acgactagtt
     4081 caaaagctaa aacttcaagt aaaaagttat tcaagaaaat ctagaaaata ctcatcctac
     4141 aaaggacagg taggaaaaat atcagacaac aaaataaaaa gaaatattta ttaccgaaaa
     4201 aaaggggaaa tcgagtcaag taccaacagg ctcatctatt ccaaaaagat atttactcga
     4261 tggaaaaact cctagaatta gtgctacaaa tattgacaat ggtatcttag gttactacga
     4321 aga
//
LOCUS       DAVKMU010000090         4315 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKMU010000090 DAVKMU010000000
VERSION     DAVKMU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.090"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            64..567
                     /locus_tag="VW343_002025"
     CDS             64..567
                     /locus_tag="VW343_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEU9756514.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
     gene            697..2649
                     /locus_tag="VW343_002026"
     CDS             697..2649
                     /locus_tag="VW343_002026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU9756515.1"
                     /translation="MDAGIGTGTNINAGLTEAQRLLQSARAGAKKVVILLSDGEANMY
                     YESNSGRTIYNYYSNPNVGRMIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYI
                     GSNDSITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVL
                     SKYVQLLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLED
                     DAKYVLKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRT
                     KIKDYSEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSY
                     RKVTSPVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQ
                     LPSLTVKKILEGEQSPNKSFTINVTLSDKDGKPINGKFGNTTVTNGKAQISLKNSQET
                     ALSYLPRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVIKKVTG
                     AFANLLQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKIL
                     DLPLNARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTL
                     VLGVVLPLGGIFFIILLGHLVVNRRK"
     gene            2649..3200
                     /gene="sipA"
                     /locus_tag="VW343_002027"
     CDS             2649..3200
                     /gene="sipA"
                     /locus_tag="VW343_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HEU9756516.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            3245..>4315
                     /gene="pitB"
                     /locus_tag="VW343_002028"
     CDS             3245..>4315
                     /gene="pitB"
                     /locus_tag="VW343_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HEU9756517.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNK"
BASE COUNT         1517 a          780 c          871 g         1147 t
ORIGIN      
        1 tcttatggaa gtctcagatt tatatagtat cagattaaaa catattctag caagggagat
       61 agcatgaacg ttcaatatga ttttaagaag attcaatatt ttaccagtag tttagttatc
      121 tttctcgcta ttcttttttt gtgtgcacca attaattctt tacgtgcaga ttcaataact
      181 gaacctcaga caactctgca caaaacgatt actccgatat cagggcaaaa agaccagtat
      241 gagttgtcac tggatatcac atctaaactg ggaacggaga cccagtcaga acccttggat
      301 gtagtcttgg ttgccgatct ttcagggagt atggaagagc gagatgtgtg gtcttactct
      361 agtagacgat acattagtag gattgaagca ctaaaacata cactgaaagg tgtgaatggt
      421 cgtcaggggc tcattgatac aattctttct aattcccaaa accgtctgtc tatagttggt
      481 tttgccggaa agattgataa tcagtataat gaccgttatt ataatgaata ttatctgagt
      541 tatcaatatg gaacttggcc aaattgagct ggttggtatt caaatatctc ttcatatgat
      601 gatgctaaaa ctttagtatc ttggagcacg gattctaata gctcaaaaaa tattgttagt
      661 tcgttaacaa ttgctgactc tagtcgttct tatggtatgg acgcgggcat tggcactggg
      721 acaaatataa atgctgggtt aactgaagct caaagattgt tgcaaagtgc aagggctggg
      781 gcaaaaaaag tagttattct gctgtcagat ggcgaagcta atatgtatta cgagtctaat
      841 agtgggagaa caatatataa ctattattct aatccaaatg tgggacgtat gattgatact
      901 ccatattggt ttacctctgg tttagagaga ggaatgctga atatatctag tttaatagct
      961 ccaaaaatag atggctttta ttcaatcaaa ttcagatata taggttcaaa cgatagtatc
     1021 acatctctta aaggatatat cagtggttat aattctggaa tccccaacga aatattttct
     1081 gccaataatg aaaatgactt gcaacaaaaa ttcaaagaaa tcacagataa aattctacct
     1141 ctaggcgtac accatgtaac tatatcagat gtcttgtcca agtacgtgca gctgttacct
     1201 ggtgatgctt cacaccttcg tgtcgtcaaa atcaaggatg gtaacgagca agaactgaat
     1261 gacaatcaag ttacgattga aactaagaag aacgaacagg gattagtgga agtaacagcc
     1321 aagtttaatc cgagttacac tttagaggat gacgccaagt acgttctcaa gtttactgtc
     1381 acctctagcc aagaggcatt tgatgcgatt gcgggtgata agacacttac tagtgatgat
     1441 gccgaagaag ccgatgctac taaactctac tccaacaagg gggcaaaagt tgcctattcc
     1501 tatggtattg ggacctcacg taccaaaata aaagactatt ctgagaagcc cactttcaag
     1561 ccgtcagatc cattgacggt tcctgtagag attgagtgga aaggtgtgga tggaaaatca
     1621 aatccatcag caaatcgtcc acctagtgtc gaattaaact taaaccaaaa gaaagatgga
     1681 agtataaagg attcctatcg aaaggtcact agtccagttc aaacgaatag ttttactgaa
     1741 aatactagtt ttgcaaaggt agctaaggga tatgactacg aactgaaagc accagacgct
     1801 ccgggataca cagtcgaagt tcaaaagaca ggtacgaaag agaaaccatc cttcaaagtt
     1861 atttaccgac agcttccaag tctcaccgta aagaaaatcc tagaaggtga acaatcacct
     1921 aataaatctt tcacaattaa tgttaccctt tcagataagg atggcaagcc gattaacggc
     1981 aagtttggga atacaacagt gactaacggg aaagcacaga tttctctcaa aaatagtcag
     2041 gaaactgccc tcagttatct gcctcgtgat acccactata aggtggaaga agtagagaac
     2101 tctagaacgg gatatcatgt cacctatgaa aaacaagagg ggactttgtc agaggatgtt
     2161 caaacaatcg tcaccaacca cagacttccg acactttcag tcataaaaaa agttacaggt
     2221 gcttttgcta atcttctgca atcctttaag attaccatta acgtaaagga tgcgcaaaat
     2281 aaaccattga atggatcgta tagtgcaata gtaaataatc aaaaaacaac gctacaattc
     2341 accaatggta aggcgacagt tgatctaaag aaagataaaa ccatcaagat tctcgacctt
     2401 cctctaaatg ctcgttatag tatcgaagaa gaagcaagtt cgtctcgtgg gtatcaggtg
     2461 tcctatgata aaaaagaagg aactcttgat gcaaataagt ctgcgacagt cacgaataat
     2521 aaaaacagcg tacctgaaac gggaattgac ttcttgagta gcactctcgt gcttggagtc
     2581 gttcttcctc taggagggat cttctttatc atcttacttg gtcaccttgt ggtgaatagg
     2641 aggaagtgat gctgcttaaa aagaaacata agaaaccagt aacacaagtc aatcgggata
     2701 agtctccgcc gagtgtctgg ggagatatcc tttacttagt cagtaaactt ctgatggttg
     2761 gatttgtact agccatcctt tactttttcg tctttggatt attaagatac aatgacgatg
     2821 gcatgaagcc cgccttaaaa gatggcgact tggtcgtcta ttataggttg gataaacgct
     2881 attcgattgg tgatttgcta gtctatagtt ataaaggtaa ggaaagagtg gcgcgtgtca
     2941 tagcaaccga aggaagtaca atcgatataa acgaaaatgg tctcatcatc aacggttctc
     3001 ctcaacaaga gcaagatatc tacaaagaaa cgctgctcta taaggaaggg gcaaccttcc
     3061 cgatgaaagt cccagcagga caactttttg tcctcgggga caatcgaaca acggctgtag
     3121 acagtcgtgc ttttggaacc atccctatac aggatactca aggcaaagtt gtaacagtca
     3181 ttagaagacg aggcttttga tgatcataat gaaaaaagaa aataaaaaaa caaaagaaat
     3241 aatcatgaaa aaaacattct ttaaaaagct attcactgca agcattgcag ctataaccgc
     3301 tttgtccgta ttcagaggtg tcccgacttt tgcggatgat aattcagcaa taaccaaagc
     3361 aaatggtgaa aataatgctg ttgtgaagat taataaaacg ttgaatattg cagagggaat
     3421 aacaacacca acagcgacat ttacatttaa gtttacagaa aaaacaggac aatcttctaa
     3481 cggtgcgcca tatcaaaccg gagttgcaat tccagataga aatgtagaat acaataaaaa
     3541 tgatcaccca actgctgata agattcaaaa agcaacagaa gacatttttt cgggagttgc
     3601 ttatggccat gctggtgaat acgtttatga tgtagcggaa gcaaaaactg gatggcaggc
     3661 gattaccaaa aatggtaaaa caattgatgc catgagatac gacaaacgta catatgaaat
     3721 gcacgttatt gttaagaata aagtaaatgg tggtgtctat atttcatcag tatactttaa
     3781 ggaaaataat aaatctaacg cccctaaagt agaaccaagt gaacaaggcg tttataattt
     3841 atttgataac acatatacca aagacgcaag taaggagcct aatcctgatg atccgagtca
     3901 agtagacccc aatgcgaaag cattaacaat tactaaaaaa gttgatggag cttcagggga
     3961 taaaacaaga gatttccaat tccatatcaa gattcaactt ccaagtacaa ataaaacagc
     4021 agaaacccct gttacgaata ttatagtaaa acatggatct aagtcagagg tgttggcagt
     4081 agtgacccca gcagatacag ttgagtacaa ttttactctt aaagatggtg aaacatttac
     4141 agttgaacaa ctaccagcag gttctaaata tacagtaact gaaactggag tagcaggtta
     4201 tacagattca tcaatttata ctacaaatgg tgcagaacaa acatctcaag gacaaaaaaa
     4261 tgtagatttt acattaacag atatcctcat aggtgaaaag aaaaacgaca acaaa
//
LOCUS       DAVKMU010000091         4272 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKMU010000091 DAVKMU010000000
VERSION     DAVKMU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.091"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            206..2107
                     /locus_tag="VW343_002029"
     CDS             206..2107
                     /locus_tag="VW343_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9756518.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGDELDKLMSDYDHLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSKKTSGNVVLTVENTAI
                     GYDGEVLSQPINVDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYIEKRATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            2110..2973
                     /locus_tag="VW343_002030"
     CDS             2110..2973
                     /locus_tag="VW343_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9756519.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYFFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            3053..4231
                     /locus_tag="VW343_002031"
     CDS             3053..4231
                     /locus_tag="VW343_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9756520.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGFIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSIHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
BASE COUNT         1280 a          719 c          919 g         1354 t
ORIGIN      
        1 gcgtacgtaa ataatgaaaa gaattaaata ataggcttgt gcgataacta tttgaaacaa
       61 gattagctgg ttagaaaatc caacaagata gtgaaggaat ctaatataaa atatacataa
      121 tttagtttat tttatgttat aaaagttgta ctaaaagact tacgattatc ttgaatgact
      181 tagggaaaca tgctatacta cttgtatgat tattttacaa gctaataaaa ttgaacgttc
      241 ttttgcagga gaggttcttt tcgataatat caacctgcag gttgatgaac gagatcggat
      301 tgcccttgtt gggaaaaatg gtgcaggtaa gtctactctt ttgaagattt tagttggaga
      361 agaggagcca actagtggag aaatcaataa gaaaaaagat atttctctgt cttacctagc
      421 tcaagatagc cgttttgagt ctgaaaatac catctatgat gagatgcttc atgtctttga
      481 tgacttacgt cggacagata aacaacttcg tcagatggag ttggagatgg gtgaaaagtc
      541 tggcgatgaa ttggataaac tgatgtcaga ttacgaccac ttatctgaga attttcgcca
      601 agcaggcggc tttacctatg aagctgatat tcgagcgatt ttgaatggat tcaagtttga
      661 cgagtctatg tggcagatga aaatagctga actttcaggt ggtcaaaata ctcgtttggc
      721 tctagctaaa atgctccttg aaaagcccaa tctcttggtc ttggacgagc caactaacca
      781 cttggatatt gaaaccatcg cctggctaga gaattacttg gtaaactata gcggtgccct
      841 cattatcgtc agccacgacc gttatttctt ggacaaggtt gcgacaatta cgctagattt
      901 gaccaagcat tccttggatc gctatgtggg gaattactct cgttttgtcg aattgaagga
      961 gcaaaagcta gctactgagg caaaaaacta tgaaaagcaa cagaaggaaa tcgctgctct
     1021 ggaagacttt gtcaatcgca atctagttcg tgcttcaacg actaaacgtg ctcaatctcg
     1081 ccgtaaacaa ctagaaaaaa tggagcgttt ggacaagcct gaagctggca agaaagcagc
     1141 caacatgacc ttccagtcta aaaaaacgtc gggcaatgtt gttttgactg ttgaaaatac
     1201 tgctattggt tatgacgggg aagtcctgtc acaaccgatt aacgtagatc ttcgtaagat
     1261 gaatgctgtc gctatcgttg gtccaaatgg tatcggcaag tcaaccttta tcaagtctat
     1321 tgtggaccag attcctttta tcaagggaga aaagcgcttt ggcgctaatg ttgaggttgg
     1381 ttactatgac caaacccaaa gcaagctgac accaagtaat acggtgctgg atgaactctg
     1441 gaatgatttc aaactgacac cagaagttga aatccgcaac cgtcttggag ccttcctttt
     1501 ctcaggagat gatgttaaaa aatcagtcgg catgctatct ggtggcgaaa aagctcgttt
     1561 gcttttagct aaattgtcta tggaaaacaa taactttttg attctggatg agccgaccaa
     1621 ccacttggat attgatagta aggaagtgct agaaaatgcc ttgattgact ttgatggtac
     1681 cttgcttttt gtcagtcatg atcgttactt tatcaatcgt gtggcaactc atgttttgga
     1741 attgtctgag aatggttcaa ctctctacct tggagattac gactactata ttgagaagag
     1801 agcaacagca gaaatgagtc agactgagga agcttcaact agcaatcaag caaaggaagc
     1861 aagtccagtc aatgactatc aggcccagaa agaaagtcaa aaagaagttc gcaaactcat
     1921 gcgacaaatc gaaagtctag aagctgaaat tgaagagcta gaaagtcaaa gccaagccat
     1981 ttctgaacaa atgttggaaa caaacgatgc cgacaaactc atggaattac aggctgagct
     2041 ggacaaaatc agccatcgtc aggaagaagc tatgcttgag tgggaagaat tatcagatca
     2101 ggtgtaaaga tggaacatct tggaaaagta tttcgtgaat ttcgaacaag tggaaattat
     2161 tctttaaagg aagcagcagg cgaatcctgc tctacctctc agttatctcg ctttgagctt
     2221 ggggagtctg acctggcagt ctcccgtttc tttgagattt tggataacat tcatgtaaca
     2281 atcgaaaatt tcatggataa ggcaaggaat tttcataatc atgaacatgt gtctatgatg
     2341 gcacagatta tcccacttta ctattcaaac gatattgcag gttttcaaaa gcttcaaaga
     2401 gaacaacttg aaaagtctaa gagttcgacg actccccttt attttgagct gaactggatt
     2461 ttgctacaag gtctgatttg tcaaagagat gcgagttatg atatgaagca ggatgatttg
     2521 gataaggtag cagattattt cttcaaaaca gaagaatgga ccatgtatga gttgattctt
     2581 ttcggtaacc tctatagttt ctacgatgta gactatgtca ctcggattgg tagagaagtt
     2641 atggagaggg aggaatttta ccaagagatt agtcgccata agagattagt tttgattttg
     2701 gccctcaatt gttaccagca ttgtttagag cattcttctt tttataatgc caactatttt
     2761 gaggcttata cagagaagat tattgacaaa ggtattaagc tttatgagcg taatgttttc
     2821 cattatttaa aaggttttgc cttatatcaa aaaggacagt gtaaagaagg ctgtaagcag
     2881 atgcaagagg ccatacatat ttttgatgtg ttaggtcttc cagagcaagt agcctattat
     2941 caggaacact acgaaaaatt tgtcaaaagt taattttccc aaataaggga aaaaataaaa
     3001 agctcctttc ggttttgata caatagtttc aaaatttgag aggagcttta atatgaatcg
     3061 ctatgcagtg cagttgatta gccgtggggc tatcaataaa atgggaaata tgctctatga
     3121 ttatggaaat agtgtctggt tggcttctat ggggactata ggacagacag ttttaggaat
     3181 gtatcagatt tctgagctcg tcacatctat tctcgtcaat ccctttggcg gagttatttc
     3241 agaccgtttt tctcgtcgta agattttaat gacggcagat cttgtttgtg ggattctttg
     3301 tctggctatt tctttcataa ggaatgatag ctggatgatt ggcgctttga ttgttgctaa
     3361 cattgtgcag gctattgctt ttgccttttc tcgcacagcc aataaagcta tcataactga
     3421 agtggtggag aaagatgaga ttgtgatcta taattctcgc ttagagctgg ttttgcaggt
     3481 tgtaggtgtt agctctcctg ttctttcctt ccttgtttta cagtttgcaa gtctccatat
     3541 gacgctactg ctagactcgc tgactttttt cattgctttt gttctagtgg ctttccttcc
     3601 aaaagaggaa gcaaaagttc aagagaaaaa ggcttttact gggagagata tttttgtaga
     3661 tatcaaggat gggttacact atatctggca tcagcaagaa attttcttcc ttttgctggt
     3721 agcttccagc gttaatttct tttttgcagc ttttgaattt ctacttccct tttcgaatca
     3781 gctttacggg tcagaaggag cctatgcaag tattttaact atgggggcta ttggtttcat
     3841 cattggggct cttctagcta gtaaaattaa agctaatatt tataatcttt tgattttact
     3901 ggctttgaca ggtgtcggag tttttatgat gggattacca cttccaactt ttctttcctt
     3961 ttctggaaat ttagtttgtg aattgtttat gacgattttt aatattcact tttttactca
     4021 agtacaaacc aaggttgaga gcgaatttct tggaagagta ctgagtacaa tttttacctt
     4081 agctattcta tttatgccta ttgcaaaagg atttatgaca gtcttgccaa gtatacatct
     4141 ctcttctttc ctgataattg gaagtggtgt tatcatcttg tcttgtatat cgtttattta
     4201 tgttcgaact cattttgaaa aattgatata aggttgcttg agtttcaaaa ataatttcaa
     4261 actaagtgtt tt
//
LOCUS       DAVKMU010000092         4013 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKMU010000092 DAVKMU010000000
VERSION     DAVKMU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.092"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(148..1209)
                     /gene="dinB"
                     /locus_tag="VW343_002032"
     CDS             complement(148..1209)
                     /gene="dinB"
                     /locus_tag="VW343_002032"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9756521.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            1558..3882
                     /gene="pflB"
                     /locus_tag="VW343_002033"
     CDS             1558..3882
                     /gene="pflB"
                     /locus_tag="VW343_002033"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9756522.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1208 a          846 c          778 g         1181 t
ORIGIN      
        1 agaaaaacct tgaaggggca actcttcaag gttttatact cttagaaaat ctcttcaaac
       61 cgcgtcagct ttatctgcaa cctcaaaaca gtgttttgag cagcctgcgg ctagcttcct
      121 agtttgctct ttgattttca ttgagcttta aaatccagtc agggtaatcc ccaataggcg
      181 gacacctctt tctttctcgc ttaattcttc atagagttgc agggctattt ggcttatctg
      241 actagcatct tgtgtttttt gagcaagact ttttcgtttg gtaagagttg aaaagtcctc
      301 gtagcggatt ttcaaaatga caatttttcc agctttttct tgttgatgta gattgagagc
      361 gactttttct gatagaagag tcagctcttt tttgatatct tcctcagcaa ggagaatctt
      421 cccgtaggtt ttctccttgc cgattgattt acggatgcga ttggatttga ctggagagtt
      481 gtgaatgcca cgagcctttc gatacagatc atagcctagt ctaccaaaac ggtctattag
      541 ggttacctca ggaacttcaa gtaaatcagc accagtaaaa acgcccattt gatgaagacg
      601 ttctactgtc ttttttccta ctccatgaaa tttggaaata tccatttgtt tgagaaaatc
      661 ctcagcctgt tcaggtagaa tcactgtcaa accatgtggt ttttgataat cactcgccat
      721 tttagctaag aatttgttgt aagaaacgcc tgcggaagca gttagatgga gttcttgcca
      781 gatatctttt tgaatgaggc gagcaatttt gaccgctgac ttgataccga gtttattttc
      841 tgtcacatcc aaataggctt cgtcaatgct catgggttca atcaaatctg tatagcgctt
      901 aaaaatagct cgaatctgga gtcccacaga cttgtatttc tcataattcc ctgagataaa
      961 gacagcctgg ggacaacgtt cataagcttc cttggaactc atggcagaat ggacaccaaa
     1021 agctcttgcc tcataactac aggtagaaac gactccccgt ccacctgttt gccgagggtc
     1081 gcttccaata atgacaggtt ttcctctgag tttaggatta tccctgattt ccactgcagc
     1141 aaaaaaggca tccatgtcaa tatggatgat ttttcttgac aaatcattta acaaaggaaa
     1201 aatcaacatg cctagcacct ttttatactc ttcgaaaatc tcttcaaacc acgtcagctc
     1261 tatctgcaac ctcaaaacag tgttttgagc aatctgcggc tagcttccta gtttgctttt
     1321 cgatttccat tgagtgttac tgcttatttt cttttattat accctttttt ctgaaaaaaa
     1381 gaaaaaagga ctttattttt tcaaaaatat aatacagttt gaaataaaat atagactgtt
     1441 ttagaaaaga aagtgtaaaa atagggaatt ttcacttgtt gaaatcggtt actatatggt
     1501 atacttgtct tatgaatgta acagatgact gttactagaa aaaaagagga cattaatatg
     1561 gttgttaaga cagttgttga agcacaagat atttttgaca aagcttggga aggcttcaaa
     1621 ggcgtagatt ggaaagaaaa agcaagtgta tcacgatttg tacaagctaa ctacacacct
     1681 tatgatggag acgaaagctt ccttgcagga ccaacagagc gttcacttca catcaagaaa
     1741 attgtagaag aaactaaagc acactacgaa gaaactcgtt tcccaatgga cactcgtcca
     1801 acatctatcg ctgatatccc tgctggattt atcgacaaag aaaatgaagt tatctttggt
     1861 atccaaaatg atgaactctt caaattgaac ttcatgccaa aaggtggtat ccgtatggct
     1921 gaaactactt taaaagaaaa tggatacgaa ccagacccag ctgttcacga aatcttcact
     1981 aaatatgtaa caacagttaa cgacggtatt ttccgtgcct acacttcaaa tattcgtcgc
     2041 gctcgtcatg cacacactgt aactggtctt ccagatgcat actcacgcgg acgtatcatc
     2101 ggtgtttacg cacgtcttgc tctttacggt gcagactact tgatgcaaga aaaagtaaac
     2161 gactggaatg caatcaaaga aatcgatgaa gaaacaatcc gtcttcgtga agaagtaaac
     2221 cttcaatacc aagcattgca acaagttgtt cgcctgggtg acctttacgg ggttgatgtt
     2281 cgcaaaccag cgatgaacgt gaaagaagca atccaatggg ttaacattgc tttcatggct
     2341 gtctgccgtg tgattaacgg tgctgctaca tctctaggtc gtgtaccaat cgtattggac
     2401 atctttgcag aacgtgacct tgctcgtggt acatttactg aatcagaaat ccaagaattc
     2461 gttgatgatt tcgttatgaa acttcgtaca gttaaatttg ctcgtacaaa agcttatgac
     2521 caattgtact caggtgaccc aacctttatc acaacttcta tggctggtat gggtaacgac
     2581 ggtcgtcacc gtgttactaa gatggactac cgtttcttga acacacttga caacatcggt
     2641 aactcaccag aaccaaactt gacagttctt tggactgaca aattgccata caacttccgt
     2701 cgctactgta tgcacatgag ccacaaacac tcttctatcc aatacgaagg tgtaacaaca
     2761 atggctaaag acggatatgg tgaaatgagc tgtatctcat gctgtgtgtc tccacttgat
     2821 ccagaaaatg aagaacaacg ccacaacatc cagtacttcg gtgctcgtgt aaacgttctt
     2881 aaagcccttc ttactggttt gaatggtggt tacgacgatg ttcacaaaga ctacaaagta
     2941 tttgatatcg aaccaatccg tgacgaagtt cttgaattcg aatcagttaa agcgaacttt
     3001 gaaaaatctc ttgactggtt gactgacact tacgtagatg ccttgaacat catccactac
     3061 atgactgata gatacaacta cgaagctgtt caaatggcct tcttgccaac taaacaacgt
     3121 gccaacatgg gattcggtat ctgtggattt gctaacactg ttgatacatt gtcagctatc
     3181 aaatacgcta cagttaaacc aatccgtgac gaagatggct acatctacga ttacgaaaca
     3241 atcggtgact acccacgctg gggtgaagat gacccacgtt caaacgaatt ggcagaatgg
     3301 ttgatcgaag cttacacaac tcgtctacgt agccacaaac tatacaaaga cgcagaagct
     3361 acagtatcac ttttgacaat cacatctaac gttgcttact ctaaacaaac tggtaactca
     3421 ccagttcaca aaggtgtata cctcaacgaa gatggttctg tgaacttgtc taaacttgaa
     3481 ttcttctcac caggtgctaa cccatctaac aaagctaaag gtggttggtt gcaaaacttg
     3541 aactcacttt ctagccttga ctttagttat gcagctgacg gtatctcatt gactacacaa
     3601 gtatcacctc gcgctcttgg taagactcgt gatgaacaag ttgataactt ggtaacaatc
     3661 cttgatggtt acttcgaaaa cggtggacaa cacgttaact tgaacgttat ggacttgaac
     3721 gatgtttacg aaaaaatcat gtcaggcgaa gacgttatcg tacgtatctc tggatactgt
     3781 gtaaacacta aatacctcac tccagaacaa aaaactgaat tgacacaacg tgtcttccac
     3841 gaagttcttt caatggatga cgccttggat gcattgagct aatcaagttc ggctctttgt
     3901 caactgcagt gggttgaagt cggctaagct cgagaaagga caaaattcgt cctttctttt
     3961 ttgatgttca gagcgataaa aatccttttt ttgaagtttt caaagttccg aaa
//
LOCUS       DAVKMU010000093         3632 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKMU010000093 DAVKMU010000000
VERSION     DAVKMU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.093"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..177
                     /locus_tag="VW343_002034"
     CDS             <1..177
                     /locus_tag="VW343_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756523.1"
                     /translation="AEALLEKVTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDS
                     LLALLKESQPTPIQ"
     gene            387..3506
                     /gene="phtE"
                     /locus_tag="VW343_002035"
     CDS             387..3506
                     /gene="phtE"
                     /locus_tag="VW343_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9756524.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLKEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1358 a          592 c          697 g          985 t
ORIGIN      
        1 gcggaggcct tgctagaaaa agtaacagat tctagtatta gacaaaatgc tgtggagaca
       61 ttgactggtc taaaaagtag tcttcttctc ggaacgaaag ataataacac tatttcagca
      121 gaagtagata gtctcttggc tttgttaaaa gaaagtcaac cgactcctat acagtagtaa
      181 aatgaatgga gcatatttta tggagaagta acctttcgtg ttacttctct tttttagaaa
      241 aacgtaacag aaacttgaca aaagtaattt taaatagtaa actatttact ggttaattaa
      301 atggttaaat aaccggttta gaaaactatt taataaagta aaagaagttg agaaaaaact
      361 tcatcattta ttgaaatgag ggatttatga aattcagtaa aaaatatata gcagctggat
      421 cagctgttat cgtatccttg agtctatgtg cctatgcact aaaccagcat cgttcgcagg
      481 aaaataagga caataatcgt gtctcttatg tagatggcag ccagtcaagt cagaaaagtg
      541 aaaacttgac accagaccag attagccaga aagaaggaat tcaggccgag caaattgtaa
      601 tcaaaattac agatcagggc tatgtaacgt cacacggtga ccactatcat tactataatg
      661 ggaaagttcc ttatgatgcc ctctttagtg aagaactctt gatgaaggat ccaaactatc
      721 aacttaaaga cgctgatatt gtcaatgaag tcaagggtgg ttatatcatc aaggtcgatg
      781 gaaaatatta tgtctacctg aaagatgcag ctcatgctga taatgttcga actaaagatg
      841 aaatcaatcg tcaaaaacaa gaacatgtca aagataatga gaaggttaac tctaatgttg
      901 ctgtagcaag gtctcaggga cgatatacga caaatgatgg ttatgtcttt aatccagctg
      961 atattatcga agatacgggt aatgcttata tcgttcctca tggaggtcac tatcactaca
     1021 ttcccaaaag cgatttatct gctagtgaat tagcagcagc taaagcacat ctggctggaa
     1081 aaaatatgca accgagtcag ttaagctatt cttcaacagc tagtgacaat aacacgcaat
     1141 ctgtagcaaa aggatcaact agcaagccag caaataaatc tgaaaatctc cagagtcttt
     1201 tgaaggaact ctatgattca cctagcgccc aacgttacag tgaatcagat ggcctggtct
     1261 ttgaccctgc taagattatc agtcgtacac caaatggagt tgcgattccg catggcgacc
     1321 attaccactt tattccttac agcaagcttt ctgccttaga agaaaagatt gccagaatgg
     1381 tgcctatcag tggaactggt tctacagttt ctacaaatgc aaaacctaat gaagtagtgt
     1441 ctagtctagg cagtctttca agcaatcctt cttctttaac gacaagtaag gagctctctt
     1501 cagcatctga tggttatatt tttaatccaa aagatatcgt tgaagaaacg gctacagctt
     1561 atattgtaag acatggtgat catttccatt acattccaaa atcaaatcaa attgggcaac
     1621 cgactcttcc aaacaatagt ctagcaacac cttctccatc tcttccaatc aatccaggaa
     1681 cttcacatga gaaacatgaa gaagatggat acggatttga tgctaatcgt attatcgctg
     1741 aagatgaatc aggttttgtc atgagtcacg gagaccacaa tcattatttc ttcaagaagg
     1801 acttgacaga agagcaaatt aaggctgcgc aaaaacattt aaaggaagtt aaaactagtc
     1861 ataatggatt agattctttg tcatctcatg aacaggatta tccaagtaat gccaaagaaa
     1921 tgaaagattt agataaaaaa atcgaagaaa aaattgctgg cattatgaaa caatatggtg
     1981 tcaaacgtga aagtattgtc gtgaataaag aaaaaaatgc gattatttat ccgcatggag
     2041 atcaccatca tgcagatccg attgatgaac ataaaccggt tggaattggt cattctcaca
     2101 gtaactatga actgtttaaa cccgaagaag gagttgctaa aaaagaaggg aataaagttt
     2161 atactggaga agaattaacg aatgttgtta atttgttaaa aaatagtacg tttaataatc
     2221 aaaactttac tctagccaat ggtcaaaaac gcgtttcttt tagttttccg cctgaattgg
     2281 agaaaaaatt aggtatcaat atgctagtaa aattaataac accagatgga aaagtattgg
     2341 agaaagtatc tggtaaagta tttggagaag gagtagggaa tattgcaaac tttgaattag
     2401 atcaacctta tttaccagga caaacattta agtatactat cgcttcaaaa gattatccag
     2461 aagtaagtta tgatggtaca tttacagttc caacctcttt agcttacaaa atggccagtc
     2521 aaacgatttt ctatcctttc catgcagggg atacttattt aagagtgaac cctcaatttg
     2581 cagtgcctaa aggaactgat gctttagtca gagtgtttga tgaatttcat ggaaatgctt
     2641 atttagaaaa taactataaa gttggtgaaa tcaaattacc gattccgaaa ttaaaccaag
     2701 gaacaaccag aacggccgga aataaaattc ctgtaacctt catggcaaat gcttatttgg
     2761 acaatcaatc gacttatatt gtggaagtac ctatcttgga aaaagaaaat caaactgata
     2821 aaccaagtat tctaccacaa tttaaaagga ataaagcaca agaaaactca aaacttgatg
     2881 aaaaggtaga agaaccaaag actagtgaga aggtagaaaa agaaaaactt tctgaaactg
     2941 ggaatagtac tagtaattca acgttagaag aagttcctac agtggatcct gtacaagaaa
     3001 aagtagcaaa atttgctgaa agttatggga tgaagctaga aaatgtcttg tttaatatgg
     3061 acggaacaat tgaattatat ttaccatcgg gagaagtcat taaaaagaat atggcagatt
     3121 ttacaggaga agcacctcaa ggaaatggtg aaaataaacc atctgaaaat ggaaaagtat
     3181 ctactggaac agttgagaac caaccaacag aaaataaacc agcagattct ttaccagagg
     3241 caccaaacga aaaacctgta aaaccagaaa actcaacgga taatggaatg ttgaatccag
     3301 aagggaatgt ggggagtgac cctatgttag atccagcatt agaggaagct ccagcagtag
     3361 atcctgtaca agaaaaatta gaaaaattta cagctagtta cggattaggc ttagatagtg
     3421 ttatattcaa tatggatgga acgattgaat taagattgcc aagtggagaa gtgataaaaa
     3481 agaatttatc tgatctcata gcgtaaggaa tagcagtaga aaaagtctga atcaaaaatg
     3541 aagttctctc aaaagttaga aataaaactc tgactttggg agaatttcat tttattatta
     3601 atatataaaa tttcttgaca tacaacttaa aa
//
LOCUS       DAVKMU010000094         3542 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKMU010000094 DAVKMU010000000
VERSION     DAVKMU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.094"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(238..849)
                     /gene="rpsD"
                     /locus_tag="VW343_002036"
     CDS             complement(238..849)
                     /gene="rpsD"
                     /locus_tag="VW343_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9756525.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1044..2096)
                     /locus_tag="VW343_002037"
     CDS             complement(1044..2096)
                     /locus_tag="VW343_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9756526.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2093..2791)
                     /locus_tag="VW343_002038"
     CDS             complement(2093..2791)
                     /locus_tag="VW343_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9756527.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQI"
     gene            complement(2958..>3542)
                     /locus_tag="VW343_002039"
     CDS             complement(2958..>3542)
                     /locus_tag="VW343_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU9756528.1"
                     /translation="PWSDNGDAKNPALSPLGENVKTKGQYFYQLALDGNVAGKEKQAL
                     IDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSN
                     SGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPN
                     TGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          977 a          791 c          685 g         1089 t
ORIGIN      
        1 gttcgctcac aataagagag aactttttta cgaaaatcta ttgaatatgc cataaaagat
       61 tataccacat tgtgtactat ttttggttca ttttactata tcactttgga ctatatcggt
      121 ctagttatgt ctcaacgatt atagctggat actaagagaa atcaaaaaag cactaaggga
      181 gaacgtccca aagtgcttaa tatcaaggcc tcaaagcctg tcttattcaa taaaatatta
      241 caacatcttg ttgtagaatt caacgacaag tgcttcgttg atttctgggt tgatttcgtc
      301 gcgttctggc aagcgagtca atgaaccttc caacttttca gcgtcgaatg atacgaatgc
      361 tggacgtcca agagtagctt ctactgcttc aaggattgct ggaactttca atgatttttc
      421 acgaactgag atcacttgac ctggagttac gcggtatgat gggatatcaa cgcgtttccc
      481 gtcaacaagg atgtgaccgt ggtttacgaa ttgacgagct tgacgacgag tagtcgcgag
      541 accaagacgg taaacaacat tatccaaacg acgttccaaa agaagcataa agttgaaacc
      601 taggattcca cctttgattt ttgtagcttg tacgaacaag ttacggaatt gtttttcacc
      661 tacaccgtaa gtgaaacgaa gtttttgttt ttcagccaat tgcaaaccat attctgacaa
      721 tttagaacgg ttgtttggtc cgtgttgtcc tggtacgtag ttacgacgtg ccaattcttt
      781 acctgtacct gtaagtgaaa ggccaaggcg acgagcttgt ttccaagatg gtcctgtata
      841 acgtgacata tgaatgtcct cctgatataa ataatatttc ggtggaaata gtcacttaga
      901 aagccctgat tcgtgcagat gcccttcgcc taaacagcca aggttacttg tcataagaca
      961 cctgttgacg agcttcatgc tttcctgctg atatttcaca caaaatccat tttaccatga
     1021 atagctggat ttgtaaaggg gttttaggct ttattttcac taccagagag gttgagaacg
     1081 agggtaaagg tacttcctag accgtactgg ctgctgactg tgatttcccc acccaattga
     1141 tgggccaatt cacgcgcaat cgcaagtcct aatccatgac cacctgtctt catgttacgc
     1201 gaagtttcga cacgataaag gcgtttgaaa atattttcca aatcctctgg ggcaatgccc
     1261 tgcccttcat cggtcacact gattgaaagc tggtccttct ccagcttagc caccacttcc
     1321 agcttggttc ctggagcaga atatttaaaa gcgttatcga ccagattcac caagatacga
     1381 gaaagcttag cataatctcc ctcaatccgg gcagactctg ggattacctg caagtggaca
     1441 tctcttctct cctgctcaat caaaaactga aattcactca tgcactcaat taagagcttg
     1501 tccagaaaaa tactgtcttt actggtagtt tccacctgat ttctagctgt gtttagggtc
     1561 aaaaaattca actcctcaac cagtttattg agcctctccg tctggcgtcc aatggttgct
     1621 agataatgag cttgctccga ctccttgata atcccatcca aaatcccttc taccgtcgct
     1681 tggatcgaag tgataggggt cttaatatca tgcgacaact gggcaatcat caagcccttt
     1741 tctcgttcgc tttcttccaa ggaatcaaag cttacctgca aatcatggga catctcatta
     1801 aaagtttgcc ctaattgctg aaattctaca ggaccttgaa cctccaaatt tgaaggaaaa
     1861 tccttggccg ctacccgctt ggcatgctcc ttgagtttgc ccaacgacgt aaagactggc
     1921 aataggagaa agagactaat cccagcaccg acaaggctgg caacgatggt catcccaagc
     1981 aaaaagtaaa tctcgccttt cgcaatcagc attttttgaa cagcccaaaa aacaaccaaa
     2041 atggttaaga gggttgaaat aatatatcca accaaaatat aactttttag tttcatattt
     2101 gtcctctcgg tttctctatc ttatatccca acccccaaac tgtcttaata gtgggagttt
     2161 ggtcactact atattttgcc agctcctgtc gaagagcatg gatatgcaca ttcaaggtat
     2221 tggtgtcatc cacgtagtct tctttccaga tcttttcata gaggtctgtc ttggagaaaa
     2281 ctcgctctgg attgctagct aaaatccaca gcaattcaaa tgatttaaca gttaaatcca
     2341 gcatttcttc tcctatttga acttcatgac tactatgatt catttttaga ttgccaaggg
     2401 aaatcagctc tgtttcgccc ccacgatgaa ggcggcgcaa aatattgtgg acacgcaaaa
     2461 ccagctcacg tgggctaaaa ggcttagcaa taaaatcatc tgctcccaag ctcaggccgt
     2521 aaatcttgtc ctgttcactg gtcttagcag taataaatag gaaaggctgc tctggtgata
     2581 agtattgaac ctcactgatt aaatcataac catccatccg aggcatcatg acatctgtga
     2641 taatcaaatc aatcggtttt ttcttgaaca gctctagcgc ttccagtcca tcatgggcta
     2701 ccaagacctg ataacctgcc tgaattaagt atctctgatg aatatctgtg atttctacct
     2761 cgtcgtcaac gagtaaaatt gtctttccca tctgtctctc ctttgataaa aacagtgcta
     2821 tactgcttta agtataacac tatttttatt gttttacgat gattttaatc tgctattgaa
     2881 agaaagtcct gattgctgat tgagttgtta aactaatctt agtcagccca ctatccatca
     2941 tttatttcct tagctgatta gttttcttct tttttgtttt tcaaacctag accaccgagt
     3001 aaacctgcaa gcgcaagccc aaggaaacca atacttgcca ttgatgtttg agtctcacca
     3061 gtatttggta gcatagcttt atcctctgac atcatcgtat cagacatctt gttagcagaa
     3121 gcagccatgt tttcacctgc catcgtgttg gtagaacttg tcatggtgtc agcaggcatg
     3181 ctatctgtag tacctgtagc atgattgtga ttcatcggag tcacgccaga accagagtta
     3241 gaaggtgata atgaaccatt tgctgtgtct gatgtttctt taacatttat cttaatagtg
     3301 acttttttag ttgctacgat gttgtccaaa tctggtttac cgtctttgtt accatagaca
     3361 ttgactgtag cgctgtaagt ttgagtacca tttgctcgga actggtcaat gagcgcttgt
     3421 ttttctttgc cagctacatt tccgtccaag gctaattgat agaagtattg acctttggtc
     3481 ttcacgtttt cacctagtgg agatagagct gggtttttag cgtcgccgtt atctgaccat
     3541 gg
//
LOCUS       DAVKMU010000095         3541 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKMU010000095 DAVKMU010000000
VERSION     DAVKMU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.095"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            62..622
                     /locus_tag="VW343_002040"
                     /pseudo
     CDS             62..622
                     /locus_tag="VW343_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            727..1075
                     /locus_tag="VW343_002041"
                     /pseudo
     CDS             727..1075
                     /locus_tag="VW343_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(1346..1567)
                     /locus_tag="VW343_002042"
     CDS             complement(1346..1567)
                     /locus_tag="VW343_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756529.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            1532..2161
                     /locus_tag="VW343_002043"
     CDS             1532..2161
                     /locus_tag="VW343_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756530.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            complement(2197..3420)
                     /gene="pepT"
                     /locus_tag="VW343_002044"
     CDS             complement(2197..3420)
                     /gene="pepT"
                     /locus_tag="VW343_002044"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9756531.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
BASE COUNT         1205 a          661 c          670 g         1005 t
ORIGIN      
        1 aatttctata atcatttaat aatgaagaca tcatataaat aattatagaa atgaggtaat
       61 tatgaaattt aaaaagaaat atatagcagc tggatctgtt gttatccttt ccttaagtct
      121 gtgtgtttat gctctgaacc aacatagcta acaggccaat acagataaaa atcgtgtttc
      181 atatgtaaac agtaataaag acactaagaa gactgaaaat ttgactccag actaggttag
      241 tcaaaaagaa ggaattcaag caaaacaaat tgttattaag attacagatc aaggttatgt
      301 aacttctcat ggcgatcatt atcactacta taatggtaaa gttccttttg atgcgctctt
      361 tagtgaagag ccattaatga aagcccccaa ctatcagccg aaagatcaag atattgtcag
      421 ttaggttaag ggaggatata ttattaaggt agatggaaag tattatgttt accttaaaga
      481 tcaagctcat gcagaaaatg tacgaacaaa agatgaaatc aatcgccaaa aacaagaaca
      541 tggtaaagac gataaaggag ctagtgcaga agtatctgta gcaaaattac agggacgtta
      601 tactaccgat gatggttata tatagtgagt tgaatttaga atagcacatc atagtttcta
      661 aatcattgtt agaaattact ttaaaatccc cttatcaatt tgctcatatt ctatttcaat
      721 ctactaatag taccacatgg tggtcactat catttcattc ctgagagtga cttgtcggca
      781 ggtgaattag cagccacata tgtctttaac cctaatgata ttgtcagaga tataggtgat
      841 gcttatatag taagacatgg ggaacactat cactatattc ctaagtcatc tcttaacaat
      901 cccccatcac actctaacac ggaagaggta ggaagttctt ctagttctgt attaagtaat
      961 ccgtcactac atgtccacca tgaggaagaa gatggtcatg gtttttgatg ccaatcgaat
     1021 tataagtgaa gattcagaag gttttgtgat tcctcatggc gaccacaatc actacatcaa
     1081 ggttcaaact aagggatatg aagcggcttt gaaaaacaag attccatccc tgcaatccaa
     1141 ctatcaacct ggaacttttg atgagaaggc agtcttggca aaagtagatc aacttctagc
     1201 tgatagcaga agcatctaca aagacagatt gagttagctt taggtcagtt tactgaaagt
     1261 ttgaaaaaaa taaaagttag ttgattgatt acttctctaa attttgggca cactaaaaag
     1321 aggtcttgac acctaaactt taaaattaaa gcatattgaa tccgtgctgc tgtgcgaagc
     1381 gaatagcgtc ttggacgaac attgtcttat ctggatttct tatttctgta aggccttgta
     1441 atcttggatt ataactctcc tgttttagga gtacatggta gatagcaacc aataatctgc
     1501 gacagatagc aaggaagaag tagaaagtct tttgaacaag gcaaatcaac tcttggaaca
     1561 aatccatgaa gaaggaatca gacaatcctt ggcagaagaa gtagaaaatc taaaagctgc
     1621 cacaaacaag gctgatgcag acttggatga agtaaacagt caggtaaaag atgttttgac
     1681 tcgtatcgct agcgcccttc aacaagaaaa ggaaaatgct gagcaagatt ctcagacact
     1741 tgtactctat caaaaactct acgatattct catgtcgctt cactcttatt tagaaagtaa
     1801 taatggatct gatgcggact ttgataaggt ggatgcatta ttagatcaac tttctgctaa
     1861 tagtaaagac aaaacagctt tacttgaatc gacaaaagct attctagttt tgaatcatca
     1921 aatccagtca aaagttagcg caagtgaaga aacaagtcca gcaagaaatg ctgaagcaaa
     1981 tagtgataac accagtgctg agaaccaacc aaacgcaact gcagaatcta acattgaaac
     2041 tgctagcgac gaaaacaaac caagcaatac aagagattct aaacctgctg aatcaacttc
     2101 agaaaatgaa acaacagaat cttctacaac tactggaaat caagaaaaac cagtagaata
     2161 aaaagaaagg cgaagtagct gagccacctc gtctttttag cctttataag ctacgatgcc
     2221 aatgatggta tcaactgcac gttccatagt ctgaaggcta acgtattcaa aacgtccgtg
     2281 catattttcg ccacctgcaa agatattcgg agttgggatt cccataaagg aaatcttaga
     2341 gccgtctgtt ccaccccgga ttggttcgat aataggcgtg atacctagat cttccataac
     2401 ggctttagca atggtaattg gagtcatatc tttttcaatg acttctttca tattgtagta
     2461 ctggtctgtc aagttgagag tgacacggtc gctcccaagt tcttcattca tcttatcagc
     2521 gatagattgc atggatgctt tacgcgcttc aaaggcatct ttttcaaaat cacgaatgat
     2581 gtagcttgca cgcacctcct caacactacc tgtcacatcc attagatggt aaaaaccttg
     2641 gtaaccttca gttaactcag gtcggtcatt ttctggaagt tgattatgaa aatcaattgc
     2701 tagctgaagg gcattgacca tctgcccttt ggcagtacca gggtggacat tacggccttg
     2761 gaaatgcaat tcagcaccag cggctgagaa agtctcgtac tgaagttcac ctagtggacc
     2821 accatcaaca gtgtaggcaa aatccacatc aaaatcttct gcatcaaatt tattggcacc
     2881 aacaccgatt tcttcatctg gaccaaaacc aacacgaatc tcacagtgct taatttcagg
     2941 atgagcagtt agatattcaa tggctgtcat aatttcagca attcctgact tgtcatcagc
     3001 acctagcaag gttgttccat ctgttgtgat gagtgtttgt cctggatatt tttcaagact
     3061 cttgaagtcg gctggatcga gtttgaaacc agaattccct agttcaatca caccaccatc
     3121 gtagttttca attacctgtg gattgactcc ttcagcatta aaatcagcag tatccatgtg
     3181 cgatataaaa ccaatcttac gtgttaaaga cggatcgttg gctggcaagg ttccaatagc
     3241 aaaaccattc ggtagatagt aaacattttg cagtccaaca cgtttcattt caggaattag
     3301 gacatttgtt gcgaagtcaa cctgactctg tgtacttgga gtagtagtag agtgttcatc
     3361 agagcgcgtg ttgaccttaa cgtaggttaa gaagcggtcc aagagatttg gataagtcat
     3421 aaaaaaactc cttttttatc ataaaataga atacaaaact tcggaaaaat atgatttgag
     3481 aaattttatg aattttgaaa aaatataata attcaaattc agaagacttt caaaagaaat
     3541 t
//
LOCUS       DAVKMU010000096         3484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKMU010000096 DAVKMU010000000
VERSION     DAVKMU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.096"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..240
                     /locus_tag="VW343_002045"
     CDS             <1..240
                     /locus_tag="VW343_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9756532.1"
                     /translation="KQVHPLFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLI
                     KRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(370..1185)
                     /locus_tag="VW343_002046"
     CDS             complement(370..1185)
                     /locus_tag="VW343_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9756533.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(1182..2087)
                     /locus_tag="VW343_002047"
     CDS             complement(1182..2087)
                     /locus_tag="VW343_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9756534.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            2477..2788
                     /locus_tag="VW343_002048"
     CDS             2477..2788
                     /locus_tag="VW343_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9756535.1"
                     /translation="MERQTLKDIEVIIVNQSTEEIASISQQMNLSFNIKIIDENSFAE
                     LSDMITGDYITFLSSNDSLFDWTFEKMYHAIKLSDSDVVLGHFADLVDGVFYFYPLGG
                     G"
     gene            2793..>3484
                     /locus_tag="VW343_002049"
     CDS             2793..>3484
                     /locus_tag="VW343_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEU9756536.1"
                     /translation="MTENLTNVQVLQKMDDTDHRIRKQYRSLFGKLFNSKLLRKINQF
                     DINNLIWRLYIHSKTATYINFPTYIYKPVIDAKPLKDSYKIILENYENRIKDCAVLEN
                     YDIERAKKQYIQELANFSAWLKNDGEYLDSDIVYHKLIAAEKGIFPFLELDRLDFTII
                     SNNCVGGLIYKQLGFQYRTPFVGLFILPDDYYKLTKHFRYYMEQELVFEEELISPSWT
                     HEVYPLGHLGDI"
BASE COUNT         1176 a          580 c          674 g         1054 t
ORIGIN      
        1 aagcaggttc atcctctttt tcagactgtc tttaaaacct ttctaaagga caaagagaaa
       61 atcgtcaacg cccttcaact acactattct aacgccaaat tggaagcgac caataatctc
      121 atcaaactta tcaagcgcaa tgcctttggt tttcggaact ttgaaaactt caaaaaacgg
      181 atttttatcg ccctgaatat caaaaaagaa agaacgaaat ttgtcctttc tcgagcttag
      241 ctttttttca acccactaca gttgacaaag agccaatcta caagtcacta attttcatat
      301 aacaaaaaaa cgaacaggct ctttttctgt tattcagaag agaacttgtt cgtttttatc
      361 ttgattttct taaaaagctc tatatacata gggattttct ggtttatcga ctttggtaaa
      421 gctgtcgatt tccaaggttg gaaggttttg tttgagttct ttatggtagt gattgaccag
      481 ttttcctttg gctgtaatcc aggtgttgtt ttcatactgc cgggtattgc ccttggtcag
      541 caatccatag acaccagaat ctgcgataca gtggataatg ccgaatcgga acagaaattg
      601 actattggca tgactggggt cgttatagac aaagcctgta aactggattg tcttgccctc
      661 aaattcatct ggatagtcgt agatagcctc catgacttcc atatagtttt cattagtgat
      721 ctgaatacta tcttgggata agtatttatc cgccgccgtt cgcatttcct tttcataggc
      781 tgattttgaa aaataagaac tagtatctgg tttcaaatat tggcttgtcg tcccttcact
      841 tgtctgaata gctagatccg ttccttccga tagggggaaa tgataacctt tagcagaaac
      901 ggtctgagaa tccaagctaa cagttgggaa agttaagccg atgacaagtg gaatagccag
      961 aagagaaata ctcgtcatct tggccaatcg gctgttcaga tgactgtggg ttttgacttg
     1021 cttcatccag atatacaatt gaacaattgc caagataaag gaaagcacca tggagatata
     1081 ggccagatag gaatagtgca tgttgatgta ctggtttagt ttgcccgaca gatggaggta
     1141 aatagtcagt tcaaaatagc cagctaaaac taaaaatcga atcataggat gactccaatc
     1201 aagagagaat aggctaaaac gaccagagtc acaatcgtaa tgaagtgact aataaatcgt
     1261 gctttcaagt aatttttcat catgagaata tttttgatat ccagcattgg gccaatgacg
     1321 agaaaggcca gaactggtgc caaaccgaaa ctcgagagta gagaagcacc tataaaggca
     1381 tccgcctcac tacagagcga aagaagaaag gctaaaagca tcaagagcag gatagcaaaa
     1441 agaggagtcg cactgataga ggtcagaatc cgagtcggaa cgtagacctg tattatagaa
     1501 gcaaagaggc agccaaatac caaataacgc cccgtatcaa aaaattcatc aatggcctgc
     1561 acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag aaaaatcatg ctcatgacaa
     1621 gccagacgat tttctttctg aatcggttct tgccagaaaa atcctagaaa aattcctagt
     1681 attacagcca caagaatgga acccagagct cgtaataggg cgacatggaa ggagttgcca
     1741 aaggcagaat aggtcgcaaa aagaacaatg ggattgataa caggtgctgt cacaagaaaa
     1801 ggaacggccg tgtaacttgg aacctttttt tccagaaaac gattgatgat ggggacgatt
     1861 ccacattcac aagaagggaa aagtataccg acaaaggtcc caaaaaagat tctcccccaa
     1921 cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga tataaacttc aatcagccct
     1981 gagacaatgc ttcctatcag aacgaaggga agggcttcaa tgataatgga gagaaaaatg
     2041 gctccagttt gaagtacact agatgggagt tgttgaaaag cagtcatcta gtttttcttt
     2101 tctgctttga cttttttctc tttgtcatca gggaaagtaa cttgtttcaa atcagggagt
     2161 ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt ttgtaaattt gacagccata
     2221 ggtgtacctc gattctaaat tctacctcta ttatactatt attttgcgga aaagggaaaa
     2281 gttaaaattt caaattgagt agctaagaga taatatagct tgatattacc tgatatatat
     2341 cataattgtg ctataattaa ataaaaagaa aatcattatc agaaaattat gggaggtagt
     2401 caaactgact tactcaatta gtgttgtttt gttacatcaa ggagataaaa atagtttatt
     2461 agagtctttg tcaatagtgg agagacaaac attaaaagat atagaagtta ttattgtaaa
     2521 tcaatctaca gaggagatag cttccattag ccaacagatg aatctatcat ttaatattaa
     2581 gattatagat gagaattctt ttgctgagtt gtctgatatg attacaggag attatattac
     2641 gtttctatct tcaaatgatt cattatttga ttggacattt gaaaagatgt atcatgcgat
     2701 aaaattatcc gattctgatg ttgttcttgg tcattttgct gatttggttg atggagtatt
     2761 ttacttttat cctttggggg ggggataatt ggttgacaga aaatttaacg aatgttcaag
     2821 ttttacaaaa gatggatgat acagatcatc gaattagaaa acaatatcgc tcattatttg
     2881 gaaaattatt caatagtaag cttttacgaa aaataaatca atttgatata aacaacctta
     2941 tttggagact ttatattcac tctaaaactg caacctatat taatttcccg acatatatct
     3001 ataaacctgt tatagatgct aaacctctaa aagactccta caaaatcata ttagaaaatt
     3061 atgaaaatag aatcaaagat tgcgccgtct tagaaaacta tgatatagaa agagcgaaaa
     3121 aacaatatat tcaagaattg gcaaatttta gtgcttggtt aaaaaatgac ggtgaatatt
     3181 tagattctga tattgtttat cataagttaa tagctgcaga gaaaggaatt ttcccttttc
     3241 ttgagcttga tagattagat tttacgatta taagtaataa ttgtgttgga ggattaattt
     3301 ataaacagct tggttttcaa tatagaacac cttttgttgg tttatttatt cttcctgatg
     3361 attattataa acttacaaag cattttcgtt attatatgga acaagaactt gttttcgaag
     3421 aggaacttat ttcaccgagc tggactcatg aagtttatcc tttggggcac ttaggagata
     3481 taga
//
LOCUS       DAVKMU010000097         3408 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKMU010000097 DAVKMU010000000
VERSION     DAVKMU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.097"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            13..306
                     /locus_tag="VW343_002050"
     CDS             13..306
                     /locus_tag="VW343_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEU9756537.1"
                     /translation="MEEQIEKKEESIKQLQNQKRQLKKKLNEQERKARNKRLIEKGAV
                     FESIFEESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKDEIT"
     gene            616..2004
                     /locus_tag="VW343_002051"
     CDS             616..2004
                     /locus_tag="VW343_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU9756538.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEQWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDIERDKVIDNPKLFNLTNYLLTYSEIQKEKSKYLKGYAKT
                     NKEKYDFKKLTSAYSYLRKNNIETIGQLQTKIETLKSNSYRLNKKAKTIHKEMEDVEK
                     KILYYEIYKAKKEVYEEYQKKNIFTKEAFYNKHKKDIDQYKVVSGKLKKLLSDKEKLS
                     PKKWNEEKILLM"
     gene            2330..2503
                     /locus_tag="VW343_002052"
     CDS             2330..2503
                     /locus_tag="VW343_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756539.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            2689..3216
                     /locus_tag="VW343_002053"
     CDS             2689..3216
                     /locus_tag="VW343_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEU9756540.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            3343..>3408
                     /locus_tag="VW343_002054"
     CDS             3343..>3408
                     /locus_tag="VW343_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEU9756541.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRI"
BASE COUNT         1716 a          423 c          546 g          723 t
ORIGIN      
        1 aaaagattaa gtattgaaga acagattgaa aagaaagaag aaagcattaa gcaattacaa
       61 aatcaaaaaa gacagttgaa gaaaaaactt aatgaacaag aaagaaaagc aagaaacaaa
      121 agacttatag aaaaaggtgc agtgtttgaa agtatttttg aagaaagtat tgatttaaca
      181 aaagatgaat tttataagtt aataaaaacg ttaaatgatg aagaaataag attaaacata
      241 atggaaattt tagaagaaag aatagatgat aatgtagaaa aatcatctaa agatgaaata
      301 acataattgt cccttggtta caagggcgca attatacacc ctaaagggtg cttgcgtcct
      361 gcggagtcaa acccttgcag ggagcaacaa ccattcgctt tttaaaagtc aagggtaaat
      421 aaactcgcta aggctcgccc ttgactttta aaatttgaaa tgaaagtaac aattaagccg
      481 acatactgaa acaacaaaaa ttaattcagt agtcggcttt ttttattcta tcatttcaaa
      541 acgctcattc acaaagagga tataaaaaaa ctattaagcc tccacctaaa acaacaaaga
      601 aaggaagtga taaaaatggc agacagtttt catttttcag taaacataat atcaagagga
      661 aaaggcaaaa gtgcagtagc aagtgcagca tatataagtg gagaaaaaat aaaaaatgaa
      721 tgggacggag taacccatga ctacacaaga aaagaaaaaa tattagtaaa aaatataata
      781 ttgcctgatc atataccaaa agaatttaat gataggtcta ccttatggaa taaagtagaa
      841 atggcagaaa aaaattctaa tgcacaacta gcaagacaat tcataatagg actaccaaaa
      901 gagttatctt taagtgaaaa taaaaacctg gttgaaagat atataaaaga aaatttaaca
      961 tcacaaggaa tgatagtaga ttatgcaatt catgatgaga gtcaagataa aaatggaaat
     1021 atccattgtc atataatgac cataatgcga ccaataaacg aaaaaggaga gttcttagcc
     1081 aagtcaaaaa aagaatatat cctagatgaa aaaggagaaa aagtattaaa caaaaatgga
     1141 aaaccaaaga caagaaaagt agaactaaca acctggaacg atacaggcaa tgtagaacaa
     1201 tggagagaaa atttttcaga tctttgtaac aaatatttag aaagagcagg agcagaaaaa
     1261 agagtagacc ataggagttt taaaagacaa aattcagatt atctaccgac aatccattta
     1321 ggatcagcag caagtgcaat ggaaagaaag ggaatagaaa ctgacaaggg aaactataat
     1381 agagaaataa gaaaatataa tcaacttgta aaaacaataa aagaagagat aaaaacatta
     1441 aagggttgga taggaaattt attagataac ttatctactg cttatgaaaa atttaaggat
     1501 atcgaaagag ataaggtaat agacaaccct aaacttttta atctaacaaa ttatctatta
     1561 acttattcag aaattcaaaa agaaaaaagt aaatatctaa aaggatatgc aaaaactaat
     1621 aaagaaaaat atgacttcaa aaagctaaca agtgcatata gttatttaag aaaaaacaac
     1681 atagaaacca taggtcagtt acaaacaaaa atagaaactt taaagtctaa tagttatagg
     1741 ctaaataaaa aagcaaagac aatccataaa gaaatggaag atgtagaaaa gaaaattcta
     1801 tactatgaaa tctacaaagc caaaaaagaa gtttatgaag aatatcaaaa gaaaaatata
     1861 ttcacaaaag aagcatttta taacaaacat aagaaagata tagaccaata taaagtagta
     1921 agtgggaaat taaaaaaact cctatcagat aaggaaaaac taagtcctaa aaagtggaat
     1981 gaagaaaaaa ttcttttaat gtaaaatctt gaagaaataa ataaagaaaa agacaagata
     2041 aaagatgaat accaggaaat taatcatata aaatattcag tagattttgt aaacaaagaa
     2101 cttggtatag atttatccat agaaatagat aagctaataa aacagggaga aaaaccaagt
     2161 gtaatagcac agattaaaaa gttccaagac caagtcaata aagataatga atacagagaa
     2221 atgatgaaaa acaagaaaat ggatcaagaa agataagacc tggtaaaaat atcttatcca
     2281 agctataata tgaccaaaca actaaagtca gatctaggag gtaatagaga tgaataaaag
     2341 gcaagagcta atagatgaac tcataaaagc aaatgaagat ggaacataca aaacctataa
     2401 aagcacagaa gaaataaaag caatgaacaa tgaagaaata cagattatat attccaacat
     2461 gaaaaactat ctatcagaca aaagaacaca cataaactat taaaggtgga aaggtagtgt
     2521 aagctatttt taaaagtata aaaggtacaa agacctaaga aatatataaa aacatcaaaa
     2581 taaagtattc caccaccaag aacaataaaa taaaaacaag caaagggaag tatagaaaaa
     2641 ttaaaaccta tacttccctt ttttaattca aacaaaggag agaaaagcat gataaacgaa
     2701 gaaataagca aggaagcagg tcaagcagca caaaccataa tatcatacac aataaaggca
     2761 acaaaagaat caatcaattt agaaaaagaa ataagaaaaa agatgaatga aactttagaa
     2821 aaagcaaatg gaaacttaaa aagtcttatg ggcgatgaaa tgaaaataaa agatctctac
     2881 aagaaaggac aactagaaaa tataagcata gatcaaatcg acctcaaaga cttaaaaaaa
     2941 gaactaaaca aacttggagt aagtttctca gtaatgaaaa acaaagaaag caaaaactat
     3001 gaaatattct tccaagccaa agacataaaa gtaatggaat atgcctttaa gcaagtcata
     3061 gccaaggaaa ataaaaaaga aaaagaaagt atcctaaaac aaataaagaa atacaaagac
     3121 ctatccaaaa acaaagataa gacaaaagaa aaaggaaaaa ggaaagtaaa agaaaaagta
     3181 aaaccaaaca aaaaagatat gaccagagaa atctaaaggg agtaacgaaa agttacaccc
     3241 ttaaataacc ataataacaa gacttccgaa aaacagaagt ccagaattcc gaaaatcgga
     3301 aatcaagaat ttcgattatc gaaaatcaag aaaggagcag atatggcaac aaacaaaaga
     3361 tactattgga taaaattaaa agaagaattt ttcacagaca aaaggata
//
LOCUS       DAVKMU010000098         3287 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKMU010000098 DAVKMU010000000
VERSION     DAVKMU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.098"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            68..1240
                     /locus_tag="VW343_002055"
     CDS             68..1240
                     /locus_tag="VW343_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9756542.1"
                     /translation="MVIDNVKVDDKVCEIISEIMDESYTDIQILTHKLYSKGDDIKAS
                     KTIWMKKSGKEYSEAFAGTGEARIILLVNDIVNAQSNSLILIDEPEISLHPSAIYKFK
                     EFLLQECLNKKHQIIITTHSTQLIKDFLREAVKLLVKNGEKVDVIENIDYQDAFFELG
                     DVYHSRKMIYVEDRLAKYILEFVITHSGSENLKQNLVVRYIPGGANQIICNNILNSSY
                     LDSDNHYFWFDGDQNTNVSESNNLMNYLENGVVISDKIPESDNKNLDDIIKLITGCPI
                     KFNVSGNKGQKNNIELIAKQRSFIDYWAKYVSYLPFPTPEFFLANLCNSVDREGYDFS
                     KDGNGKEYFRKKTQVALGIENITSEDIFQEQRRAVSKIQPESSMFQCIKEKLEALF"
     gene            1620..2423
                     /locus_tag="VW343_002056"
     CDS             1620..2423
                     /locus_tag="VW343_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9756543.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(2539..3287)
                     /locus_tag="VW343_002057"
                     /pseudo
     CDS             complement(2539..3287)
                     /locus_tag="VW343_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1087 a          507 c          592 g         1101 t
ORIGIN      
        1 acgatctaaa ccagttgctg atattttaaa gaataataca agtagttata cgcgttataa
       61 aaaggaaatg gttatagata atgttaaagt tgatgataaa gtttgtgaaa ttattagtga
      121 aataatggat gaatcttata cagacattca aattttaaca cataagttgt attcaaaagg
      181 tgatgatata aaagcttcca aaactatttg gatgaaaaaa tctggaaaag aatattccga
      241 ggcttttgca ggaacaggtg aggctagaat tattttgtta gtaaatgata tagtaaatgc
      301 gcaatcaaat tcattaatat taatagatga acctgaaatc tcacttcatc cgagtgcaat
      361 ctataaattt aaagagtttt tacttcaaga gtgtttaaat aaaaaacatc aaattattat
      421 cactacacat tctacacaac ttataaaaga ttttcttaga gaagccgtga aacttttagt
      481 gaaaaacgga gaaaaggtag atgttattga aaatattgat tatcaggatg cattttttga
      541 attaggtgat gtgtatcatt ctaggaagat gatttatgtt gaagatagac tagctaaata
      601 tattctagag tttgttatca ctcattcagg tagtgagaat cttaaacaga atttagtagt
      661 gagatatatt cctggtggag caaatcaaat aatttgtaat aatattttaa actcatcgta
      721 tttagattcc gataaccatt atttttggtt tgatggagat caaaacacta atgttagtga
      781 atcaaataat ttaatgaact atcttgaaaa tggtgttgtt atatcagata aaattcctga
      841 atcagataat aaaaatcttg atgatattat aaaattgata acgggatgtc caattaaatt
      901 taatgtttca ggtaataaag ggcaaaaaaa taatattgaa ttaattgcga aacaaagaag
      961 ctttatagat tattgggcta aatacgtttc ttatcttcct ttcccaaccc cagagttttt
     1021 tttagctaat ttgtgtaaca gcgtagatag ggaaggttat gatttttcta aagatggcaa
     1081 cggaaaagaa tattttagaa aaaaaactca agtagcgtta ggaatagaga atataacttc
     1141 tgaagatatt ttccaggagc aaagaagagc tgtttcaaaa atacagccag aatcatcaat
     1201 gtttcagtgt attaaagaaa aattagaggc attgttctaa ttccttagat taggtactac
     1261 ataacaaatt ttttaaattc aaacccgcac caaaaacaac attgcaactt tcttctcagg
     1321 taatgtgttg ggatttgaac agaccaacga gttcatagag gtgtatgcca atgagtttga
     1381 taagtatgcg cgtcagactt attagttcat ttatccagat atatctcgat aatcatatta
     1441 tttagtcaaa gaacatccca tatgagggca tgaaatgcta agtttagctt gctcactatt
     1501 aagatgaagt atatatattt catagaactt attgtgaagg atttttataa ttttcgccaa
     1561 ttccataccc atttcaccat ttaccgatga cacagaaaaa caattgaaag gatatttcta
     1621 tgcaaccaac ttacaacatt gacaatccaa acttgtctta tgaagcgaag cgtgatttat
     1681 ggcggatagg ttttggtctg cagaaagttg acaatctagt gccatcagcg tatatggaat
     1741 ctttggctga gaaacagtcc cggggagaac tgacttatga gcaggtttat gaggatgcaa
     1801 cggcttatca ccatactatt gatgcaagta cggaagaggc agacttggtt tctctacgta
     1861 ttgtagaact attgtctcga agaggcttta gctttagtcc tgcgacctta cttgctattc
     1921 ataaggagtt gtttcaagat atatttgaac cctctattcc ggtggggcaa tttcgtcaga
     1981 ccaatatcac aaagaatgaa cctgttttga atggtgaaag tgttgtgtac tctgattact
     2041 ccatgattca aatgacctta gattatgatt ttaatcagga aaaacaagtt gcatatgcga
     2101 cactaactca ggcggatgtg gttaaacaaa tccagcattt tatttcagga atctggcaga
     2161 ttcatccatt tcgcgaagga aacactcgga cagtaacggt ctttttgatt cagtatcttc
     2221 gtgagtttgg ttttgatatt gataatacac catttcagca acattccaaa tattttcgtg
     2281 atgccttggt attagataat gcaaagattt tacagcgacg tcctgagttt ttaacagctt
     2341 tttttgaaaa tctcttgctc ggtggtcaaa atgatttgtc ttcagaaaaa atgtatctag
     2401 atttagacct tgatttttca taatccaaat actgagtaaa cattgaattt taggcaaaaa
     2461 tgaagtaaat attctcacaa gaaaacgtat atcatcaaag ttttaggtgt actgacccca
     2521 aacagttaga caattaattt atccgaagga tttagttctg tattgcacag gactaagtcc
     2581 ttttagtttt accttaattc gtttattgtt gtagtaatca atatagtcta caatagcttg
     2641 ttccaattgc ttaagcgact gaaacgactt ctcataaccg taaaacattt ccgatttcag
     2701 aatcccaaag aaagattcca tcataccgtt gtcttggctg tttcccttgc gtgacatgga
     2761 tgcttgaact cccttactct ctaggaaccg atgataagaa tcgtgtcggt attgccagcc
     2821 ttggtcacta tggagaatcg tattctcata gtgcttctct gtgaatgcct gttccaacat
     2881 tgtttgtact tgttctaagt tgggtgaagt tgaaagatta taggcgataa tttcgctatt
     2941 aaagccatct aaaactggtg ataagtaaag cttttgagta cttgctggaa tggcaaattc
     3001 tgtcacatct gtgtagcact gttccattgt tttagagcct tcaaattggc cttgaatgag
     3061 attctctgcc ttcttgccaa cgtctccttt atgagaagaa tattttcgtt tctgtcgcat
     3121 tttagcttgt aaattgagta cttcatcaag tgttgaactc ttttatgatt taccagataa
     3181 ccacgatttc ttagttctaa ataaatccga cgataaccat aatttccctt gtgttcgata
     3241 aagatggatt gaatttcagc tttaagctct tggtccttat ctgtttt
//
LOCUS       DAVKMU010000099         3084 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKMU010000099 DAVKMU010000000
VERSION     DAVKMU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.099"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..478)
                     /locus_tag="VW343_002058"
     CDS             complement(<1..478)
                     /locus_tag="VW343_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9756544.1"
                     /translation="MENFGAVLKDIRISKNFRLKDLSCNEISESTISRFENGITKLSI
                     NHFYILLNRLGISFSEFEELVHCYYSKKECLFEELEHAVNSSDIFLLQELVDKIELKQ
                     KQEKSLCNYHIKLIAEQQINRLANLPYNSSKCNELIKYLLSVDTWMEYELKLFYNSV"
     gene            572..1792
                     /locus_tag="VW343_002059"
     CDS             572..1792
                     /locus_tag="VW343_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU9756545.1"
                     /translation="MKKILLSTVALLSLVASLLANNPVSAQESSSQATYSKSSGSWIK
                     SGNRWWYKHSDGSYTTNGWEKINGTWYYFDSEGWMKTGWIKEYGKWYYLDDSGAMKTG
                     WCLVSGSWYYLNSSGVMQTGLQTINGKQYYLAAGGAMQTGWHNIGDDTYFFANSGENQ
                     NINRRALVLGETSTRAVPIADVNAMEKVFNNQNFSEVVRFPDRTKSEIIAKMQELFES
                     SSEGDVNYLYFTCHGGRDGRIYIGSDGLAFSGWELASVLKQYKGKFVVMLDCCHAGTI
                     ISKDNTGEGNEGASTEYFDLDEFVSGFSNMDGNEKSGEMIDSKFLVLCSSRGAEYSSG
                     GSLSLATKYWSLGSGWNPLQNSQAYLAADQNNNRRITLNELYTYSREQVLKQNSNQHI
                     EVYPDNSQFVLFKK"
     gene            1805..2986
                     /locus_tag="VW343_002060"
     CDS             1805..2986
                     /locus_tag="VW343_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9756546.1"
                     /translation="MKVSKKITLFSLSFAGFVLLTLPQAGKAFELKEDWAFKGGIRYE
                     NGKVSKINNGYEVNIKVLDLPSTSAIEWTVRLNGEKQNTNFLAEERTVSKTEDKGRFL
                     HFYIPYGYRGDIVVEAKSGNEVKTWSTKVVDDVYSDSAKSGYFILDGEQILESSWDSV
                     NESYIATLPTVTSGKTVVAWREKGTLNLIKPGRIARQYNSSGSYVELSPIFETASWLK
                     SNQNWYYQKQGQLVQNSWIKDQGSWYFMDDEGVMFNQTWLHQGGSWYAFKSSGAMISA
                     DWLYDNGSWYYLKDSGSMVTGWLKNGGSWYYLNKSGSMATGWIKDSGTWYYLKNSGSM
                     ATGWVKDSGSWYYLKNSGSMATGWVKDNGKWYYLASSGNMLRNTRTPDGYYVDGSGAW
                     K"
BASE COUNT         1044 a          423 c          633 g          984 t
ORIGIN      
        1 acactgaatt atagaaaagt tttagttcat actccatcca agtatctaca gaaagcagat
       61 attttattaa ttcattacat ttagatgaat tataaggaag gtttgcaaga cgattaatct
      121 gttgttcagc aatcaatttt atgtgataat tgcataaact tttttcttgc ttttgtttta
      181 actctatttt atccactaat tcttgaagta aaaatatatc tgaagaattt acagcatgtt
      241 ctaattcttc aaaaagacat tcttttttgg aataataaca atgaactaat tcttcaaatt
      301 ctgaaaatga tattccaaga cggtttaaca gaatataaaa atgattaata gataattttg
      361 taatgccatt ttcgaaacga gaaatagttg attcactaat ttcattacat gataaatctt
      421 taaggcgaaa attttttgaa atacgaatat cctttaaaac tgctccgaaa ttttccataa
      481 tgttcctctt ccatttctgc aaaaaataaa acgcttgttt tttatttatg atatgattat
      541 acaaaaataa aatagaaagg acaataatat catgaaaaaa attttattgt caacagttgc
      601 tttattaagc ttagtagcat ctctgctagc aaataatcca gtttctgctc aagaatcatc
      661 ctctcaagca acttatagta agtcaagtgg aagttggata aaatctggta accgttggtg
      721 gtataagcat tctgatggtt cttatactac aaatggatgg gaaaaaataa atggtacctg
      781 gtactatttt gatagtgaag gttggatgaa gacaggttgg attaaagaat atgggaaatg
      841 gtattaccta gatgatagtg gtgctatgaa aactggatgg tgtttggttt ctggtagctg
      901 gtattattta aatagtagcg gtgtaatgca aacgggacta cagactatca atggaaaaca
      961 atattattta gctgccggcg gtgctatgca gacaggttgg cacaatatcg gagatgatac
     1021 ctatttcttt gctaatagtg gggaaaatca aaatatcaat cgtcgtgctt tagttcttgg
     1081 tgaaacatca acgagagcag tacctattgc agatgttaat gctatggaaa aggtttttaa
     1141 taaccaaaac tttagtgaag ttgttcgttt cccagataga acaaaatccg agataattgc
     1201 aaaaatgcaa gagctttttg aatcttctag cgaaggcgat gtgaactatc tttactttac
     1261 ttgtcatgga ggaagagatg gaagaatcta tattggtagt gatggcctag cattttctgg
     1321 ttgggaattg gcgtccgttt tgaaacaata caaaggtaag tttgttgtta tgctggattg
     1381 ttgtcatgct ggaacaatta tttctaaaga taatacaggt gaaggcaatg aaggagcctc
     1441 tacggaatat tttgatttag atgaatttgt atcaggattt tctaatatgg atggtaacga
     1501 aaaatcaggt gagatgattg actctaaatt tttagtatta tgctcatcaa gaggtgcaga
     1561 atactctagt ggtggatcat taagtttagc gactaaatat tggtctttag gttcaggatg
     1621 gaatcctcta cagaattcac aagcatattt agcagcagac caaaataata atcgcagaat
     1681 cacattaaat gaattgtata catactctcg tgaacaagtt ttgaaacaaa attctaacca
     1741 gcatattgaa gtttatcctg acaacagtca atttgtgtta tttaagaaat aagggggcaa
     1801 taaaatgaaa gtatcaaaaa aaattacact atttagtttg tcttttgcag gttttgtttt
     1861 attgacttta cctcaagcag gaaaggcttt tgaacttaaa gaagactggg catttaaagg
     1921 tggcattcga tacgagaatg ggaaagtcag caaaattaat aatggatatg aagtaaatat
     1981 taaagtgtta gatttaccta gtactagcgc aatcgaatgg acagttagat tgaatggaga
     2041 aaagcaaaat actaacttct tagcggagga aagaactgta tctaaaactg aagataaggg
     2101 acgtttcttg cacttttata tcccctatgg atatcgtggg gatattgtag tagaggctaa
     2161 gagtggaaac gaagtgaaga cttggtctac taaggtagtt gacgatgttt attcagattc
     2221 tgctaagagt ggctacttta ttctcgatgg ggaacaaatc ttagaaagtt catgggattc
     2281 cgtaaatgag tcttatattg caacgcttcc aactgtaaca tcaggaaaaa ctgttgttgc
     2341 ttggcgtgaa aaaggaactc ttaatttaat taaaccagga agaattgctc ggcaatataa
     2401 tagcagtgga tcatatgtag aactttctcc aatctttgaa actgctagtt ggctaaaatc
     2461 aaaccaaaac tggtactatc aaaaacaagg tcaattagtt caaaattctt ggatcaagga
     2521 tcagggaagc tggtacttta tggatgatga aggggttatg tttaatcaaa catggctaca
     2581 ccaaggtgga agctggtatg cctttaagtc atcaggagcc atgatttcag ctgattggtt
     2641 gtatgataat ggttcttggt attacctaaa ggactcaggt tctatggtaa caggatggct
     2701 taaaaatgga ggctcttggt attacctaaa caagtcaggt tcaatggcta caggttggat
     2761 taaagatagt ggaacttggt attatctaaa gaattcaggc tctatggcga caggctgggt
     2821 gaaagatagt ggctcttggt attatctaaa gaactcaggt tcaatggcaa caggttgggt
     2881 caaagataat ggtaagtggt actatctcgc atcatctggt aatatgcttc gcaatacacg
     2941 cacaccagat ggctattatg tagatggcag tggcgcttgg aaataaattt ttaaaagaga
     3001 aaatctactg agaaggtaga ttttcttttt agttaatgaa tatagaataa agtagatcag
     3061 gactggtcta ttttattttg taac
//
LOCUS       DAVKMU010000100         2977 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKMU010000100 DAVKMU010000000
VERSION     DAVKMU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.100"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..1354)
                     /gene="rlmD"
                     /locus_tag="VW343_002061"
     CDS             complement(<1..1354)
                     /gene="rlmD"
                     /locus_tag="VW343_002061"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9756547.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1392..2168
                     /gene="recX"
                     /locus_tag="VW343_002062"
     CDS             1392..2168
                     /gene="recX"
                     /locus_tag="VW343_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9756548.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDENIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2257..2790
                     /locus_tag="VW343_002063"
     CDS             2257..2790
                     /locus_tag="VW343_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9756549.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          962 a          539 c          534 g          942 t
ORIGIN      
        1 aaactttttt tattaatttt acaacagctt cagttcgagc tgtatgtggg aacatatcga
       61 ccgactggat ataatgaaga tcatagactt ctactaagcg taccaaatca cgagccaagg
      121 tcgaaacatt acaagaaata taaaccattt tttctggtac ataagtaaga atagtatcta
      181 ataacttatc atccagacct gtacgtggtg ggtcaacaat caaagcatct gctcggtagc
      241 cttccttgta ccaacgagga ataatctctt ctgccgttcc agcttcataa tgagtattgt
      301 caaatcccat tcttttagca tttcgcttgg catcttcaat agcttctgga ataatatcca
      361 tacctctgag tgtttttact ttctttgcaa aggcaaatcc aatcgttcca actccacaat
      421 aagcgtcaat caaatggtct tctttatcaa catccagcgc ttttactgct tcgctataga
      481 ggacttctgt ttgctcagga tttagttgat aaaaagctcg aggggatagt gaaaattcat
      541 aattgagtac accttcttga atactctctt gcccccagat aatctctgtc ttttcaccat
      601 atatctcact ggttttagct gtatttgtat taacagctac tgtcacaact tctgggaaat
      661 ctttaaccaa ctcttttacc aattgagtta aattaagctg gcggtttgta acaataataa
      721 tctgaacctg tccggtcttt ctcgcgcgtc ggaccataat agtacggaca cctagaactt
      781 ttctctcatc cgtgattgga atctggtgat aagtaagtaa ttctgctaag cgattagcaa
      841 tcacttgggt ttccttatct tgtaccaggc agtctttcaa ctctactaaa tagtgagagt
      901 tttgtgcata taagcccgcc ttgacctgat ttttaaattt tcgagtctga aattgtaact
      961 tagctctgta atattttggt tcctgcattc caatagttgg acgaatttca taattttcat
     1021 atcctgcagg agcaaatttt ttcagcgctt gatgaagtaa gtccgtcttg aactccagct
     1081 gcttatcata atgcaggtgc atgatttggc agcctccgca ttcattataa atagtacaag
     1141 atggcacaat tcgaaattta gacttcttgt tgaccttcag taattttgct tcaacaaagt
     1201 tgcgtctaat agaagtaatc tgacaataga tatcttcgcc tttgagagct ccaggtacaa
     1261 agactaatgt tttttggtaa aagccgattc cctcaccatt aattcccatg cgtttgattt
     1321 ttaatggtat tttttgtttc actttcagat tcatacccct atcttatcac attttgagtt
     1381 ataatagagc tatgaaaatc acaaaacttg aaaagaaaaa aagactctat ctgatggagc
     1441 ttgataatgg cgacaaatgc tatatcaccg aagatacaat tgttcgtttt atgttatcga
     1501 gagataaggt gataagcgaa gaggaattga aagagattca agactttgct caattttctt
     1561 atggtaagaa tctggccctc taccacttat cctttaaagc acgcactgaa aaagaagtca
     1621 gagaatatct gaaaaaatac gatattgatg aaaacatcgt ttctcaagtt attgctaatc
     1681 ttaaagaaga taagtggatt aatgatggtc agtacgctta tgctatcatc aataccaatc
     1741 aactttcagg agacaagggt ccttatgtgc tgactcagaa actagctcaa aaagggattt
     1801 caaaatctac tatagaagag aacttgaaag aatttgattt ttctgaagtt gctcaacgtg
     1861 tagccaataa actattgaaa aaatatgagg gaaaacttcc agctcgtgcc ctgcaagata
     1921 agattatcca gaacttgact aacaagggct tctcttattc tgatgctaaa attgcctttg
     1981 acgacttaga tagccaagtt gaccaagaaa cgactcaaga actcatcttc aaggaacttg
     2041 ataagcaata tactaagtat gctcgaaagt atgaaggata cgaactgaag cagcgtttaa
     2101 ctcaagtttt agcacgaaag ggctacgatt tttcggatat agcaagcgct cttagagaat
     2161 atctttaata tttccaagtg aaattcacag attttaggta attttatggt acaatagtaa
     2221 atgataaact tataaattgt agaaagttgg ttagttatga agcttccaaa agaaggcgac
     2281 tttattacaa ttcaaagtta taagcatgat gggagtctcc accgaacttg gcgggacacc
     2341 atggtactaa aaacaacaga aaacgccatt attggtgtca acgatcatac acttgttacc
     2401 gaaagtgatg gtcgtcgttg ggtcactcga gaaccggcta ttgtttactt tcacaagaaa
     2461 tattggttta atatcattgc catgattcgt gataatggaa cttcttacta ttgcaatatg
     2521 gctagcccct actatctgga tgaagaagca ctgaagtata ttgattacga tttggatgtt
     2581 aaaattttta cagatgggga aaaacgtctc ttggacgttg aagagtacga gcgtcacaaa
     2641 cgcaaaatga attattctga tgacttggac tatattttaa aagaacatgt caaaattctt
     2701 gttgattgga ttaacaatgg acgtggtcct ttctcagaag cctatgtaaa catttggtac
     2761 aagcgctatg tagaactaaa gaatcggtaa agttgtcaaa ggtcaggaac aaaatcctga
     2821 cctttttttg gtagtataaa ataaattgtg taaacacaaa aaggaataaa tctattatag
     2881 cagagttaca acacattact agaaagagat ttatgcaaaa ttcgtaaaat atataaaaca
     2941 aatagatata ctgttatttt ttaatgtctt tattcct
//
LOCUS       DAVKMU010000101         2830 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKMU010000101 DAVKMU010000000
VERSION     DAVKMU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.101"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..143)
                     /locus_tag="VW343_002064"
     CDS             complement(<1..143)
                     /locus_tag="VW343_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9756550.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     GKEA"
     gene            348..536
                     /locus_tag="VW343_002065"
     CDS             348..536
                     /locus_tag="VW343_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9756551.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            693..1058
                     /locus_tag="VW343_002066"
     CDS             693..1058
                     /locus_tag="VW343_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9756552.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            1061..2728
                     /locus_tag="VW343_002067"
     CDS             1061..2728
                     /locus_tag="VW343_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9756553.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT          901 a          516 c          624 g          789 t
ORIGIN      
        1 gcttcttttc ctttctgttg ataggctagt agcagacgat tgatttgtct ttcagaaaga
       61 ttgagttcga cacaggcccg tttctttgtt ttctttcctt gggctatagc ttttatcaca
      121 agatattttt tcgtttcatt catattcagt tggatccttt tcatatgact attctaccaa
      181 atgtgacatt ttcacgttcg atttactaaa gacattatca cattcgaatc acaacacaaa
      241 taaaaaatct tccaattccc ttgcaaaaaa caagaaagtg tgataacata gtacggtatg
      301 tggtgctagc acatccgcta tattagatct aataggagga aaacacaatg gctaaagtat
      361 gttactttac aggtcgtaag actgtatcag gaaacaaccg ttcacacgcg atgaaccaaa
      421 caaaacgtgc cgtaaaacca aaccttcaaa aagttactgt tcttatcgat ggtaaaccta
      481 aaaaagtttg ggcttcagct cgtgctttga aatcaggtaa agttgaacgc gtttaataat
      541 aaaagcaaag gagaccttag ggtctttttt cttttattgt gggtataaca tcgtttgaaa
      601 aatagagtaa atatccgctg atacatcatt ctgcttttac acttgggctg aaatatgata
      661 aaatagagta tcaactagtt gaggtaaaaa ggatgactgt aaaaattaat acaaaagatg
      721 gtcaaatcga actaacagat gaagtgattg caaccgtcgt aggtggagca gcaactgaga
      781 tttttggtgt ggtcggtatg gctagtaaaa atgccctcaa agataatttc caagccctgc
      841 taggtaagga aaattattct aaaggtgtcg tcgtaaaggc agccgaagat ggcagtattg
      901 cagttgatgt atataccgtg ttaagctacg gaacaaagat tagtgaagtg tcaaaaaaca
      961 ttcaagagcg tgttgccttt agcttggaaa accagcttgg aattactgct cagactgtaa
     1021 atgtctacat tcaaaatatc aaagttgtag gagaataacc gtgtcaaaga ttactactag
     1081 cttatttcaa gaaatggtgc aggctgcatc aactcgcttg aataagcaag ctgaatatgt
     1141 caattcatta aacgtctttc cagttccaga tggagatact gggacaaata tgggaatgac
     1201 cattgaaaat ggtgctaaag aagttgcaga caagccagct tctacagttg gagaagtagc
     1261 gagcattctt gccaaagggc ttttgatggg tgcgcgtggg aactcaggag tgattacatc
     1321 tcagcttttc cgtggatttt cccaagctat caaggataaa gacgagttaa caggtcaaga
     1381 cttggctctg gccttccaat caggtgtgga agttgcctat aaggcagtga tgaaacctgt
     1441 tgaaggaacg attttgacag tttctcgtgg agctgctatc ggtgctaaga aaaaagctga
     1501 gcaaacagat gatactgttg aagtcatgcg cgcagccttg gaaggtgcta aaacagctct
     1561 agctaaaaca ccagacatgc ttccagtatt gaaagaagtt ggcgttgtgg actcaggtgg
     1621 tcaaggattg gtcttcatct acgaaggttt cctttcagcc cttactggcg aatatattgc
     1681 atctgaggac tttgtagcga ctcctgccaa catgagtgag atgatcaatg tagagcatca
     1741 taagtctgta gctggtcacg tagcgactga ggacatcacg tttggttact gtactgaaat
     1801 catggtagct cttaagcaag gtccaaccta tgctaaagat tttgactacg atgaattccg
     1861 taactacttg gatgagcttg gggattctct cctcgttgtt aacgatgatg aaattgtcaa
     1921 agtccatgtt catacagaag atccaggact tgttatgcaa gaaggtctca aatatggtag
     1981 cttggtcaag gtaaaagttg acaatatgcg taaccaacac gaagcacagg ttgagaaaga
     2041 agctactcaa gttatcaagt cggctgaaga aaaagagtat gctttgattg ctgtggtggc
     2101 tggtaaaggt ctagcagata tcttctgttc tcaaggcgtg gattatgtta tcgaaggcgg
     2161 tcaaaccatg aacccttcaa cagaagactt tatcaaggct gttgaacagg tcaatgcccg
     2221 taacatcatc ttcttgccaa acaacaagaa catcttcatg gcagctcaat ctgcggcaga
     2281 agttttggag caaccagcag tagtggtaga ggctcgcact cttcctcaag gtatgacaag
     2341 tcttcttgcc tttgatccaa gcaagtccat tgaagaaaac caagagcgta tgacagctgc
     2401 tcttagcgat gtcgttagcg gaagcgtcac aacagccgtg cgtgatacaa cgatcgatgg
     2461 cttagaaatc catgaaaacg ataatctagg tatggtggat ggaaaaattc ttgtgtcaaa
     2521 ccctgatatg caccaaacat tgactgaaac cttgaaacat atgttggatg aagacagtga
     2581 aatcgtaacc ttctatgtcg gtgaagacgg aagcgaagaa cttgccaatg aaatcgctca
     2641 agaaatcgta gaagaattcg aagacgttga agtcgagatt caccaaggtc aacaacctgt
     2701 ttacccatac ctatttagtg tggaataaga ttaatgaagg actgagaaat cagttctttt
     2761 ttctttttta gtaaatgaaa tcggtatctt tttaataaaa acaaaaataa cattcataaa
     2821 taaacgttaa
//
LOCUS       DAVKMU010000102         2786 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKMU010000102 DAVKMU010000000
VERSION     DAVKMU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.102"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(159..617)
                     /locus_tag="VW343_002068"
     CDS             complement(159..617)
                     /locus_tag="VW343_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9756554.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(614..1054)
                     /locus_tag="VW343_002069"
     CDS             complement(614..1054)
                     /locus_tag="VW343_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9756555.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1487..2353
                     /locus_tag="VW343_002070"
     CDS             1487..2353
                     /locus_tag="VW343_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9756556.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
BASE COUNT          930 a          399 c          590 g          867 t
ORIGIN      
        1 ctagaatagt acacctctgc ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc
       61 gatttatcct gttcttattt catttcacta tagtaaggtg cagcaggata aagaaaaacc
      121 gagaagtttc tttctcggat ttgcttatta tgaggttcct attttctatg ctccaagagt
      181 tgattgatgt gatctacttt tttagcctct cttttataga gaatagccca gatgatgaga
      241 tagacaatcg caaactcgat aatgagctgg aggtagaaaa tccagtggaa agggaaccaa
      301 ccagcaagag ttgctagtgg gacaaagcca gccagcataa ggaagaaatg agtcagagtg
      361 gcacgaagca tgctccagtc acggctaaat aagcggttgc caaagttgaa gagcataccg
      421 atggttgccc agataagtgt gcagtagagc aagaccaggg caccgtgaac ctgatgctga
      481 tccatcactt ggccgatgag agagtaggga tttagtggtg cgtaggtatt tggtgcataa
      541 atgagtgaaa agaggataga gaggatgagg ccgataagaa caccggtagc tgcatcgtga
      601 aatacttgtt ttttcatagt tctaatttct ccttgatggt ttttagataa cggcgtgaag
      661 agtaggtgaa gctttcgttt ttcaagaaaa tttctaccag accgtttggc gtgagcttga
      721 ggtgagagat ggaatcgata ttgatgattt ctgattggga aatttggata aaattggttg
      781 gcaagagttt aagaacctga tagagtcgca aatcaatgct gtaggtctga ctcgcggttt
      841 ctgctagaac cttccgattc tcgatataga agcgctgaat cttgccaatc tcaactagat
      901 agacctgatc atcgattttt cctttgattt tttctctttg gtccagattt tctgcgaact
      961 cgatgacttt ctggactttt tcggtttctt gaggtgcttg gacaatcagc ttttcctcct
     1021 cgtaagtctc actaatctgt agttctactt tcatagtttt ctctcctttt cagttataca
     1081 aggttgtgat cacttcctgt atatccttat taaacactat tttatagccc atggcaagga
     1141 accttgtcta tgtggaggga ttgggctcct atgtggtgga gcttttctgt tctttctgaa
     1201 atatggtata atagcactaa tcaatttcta ggaaaataga tacggaaagg ggctgaaaga
     1261 actaggggaa cagttggaat tagaaagtga taggtatgaa aagtggcgtt gaagctgaat
     1321 aagtttataa agcgtttgaa tgaagaaatg gaaagtaaat aagttgacga tgaattacaa
     1381 aatagatttt atctacaatt tgtatctttc aaatcattga tttgaatttt agatttaatc
     1441 atgttacaat taaaactgaa atcaaataga agaggaaaat gcaaacgtgg gcacactatt
     1501 agcaacaaga ttaaaaaata gacggaaaga attaaaaatg tctcagcgag aactagctga
     1561 aggtatctgt aaacaggggc aaatcagtcg attagagagc ggagagttta cccctggagc
     1621 ggatttcctg aatgcccttg ctaagaagtt aaaagtcagt atggattatt tttttgatga
     1681 gcaggttgta gagaaagttg aggagttatc agaatttaag aaattagcac agacatttat
     1741 tacaaatcgc aattatgagt ctttgaaata tatatatgaa ttggagaatg taaaagctca
     1801 ccgcctatct ttagcagata aaatctacat ggaatggata aaatctctga tagattttta
     1861 tttttacgga cagaaagaag atgcgattgt acgattagag ggggtattat cacagttaag
     1921 tgtatctgat ttgaactatc tccaagtttc aaacacccta ttcaattttt attatgatat
     1981 tggagattta ggtcgtttca ataaaattcg tgaaaaacta gagtatcaag taaatcacct
     2041 caatttgagt acaatcgaag agttagaact atctattaag tttaactaca acgtttgtcg
     2101 ttatctatgg ttacagaaaa atattgaaga agctattact aaaattacag ctactataaa
     2161 acaatgtaag gcttacagaa cgaattatct tttggctgat ttgtacttat tgatgggaaa
     2221 tgtaagtgaa aatttttctt ctaaaagttc ggtgaaagaa tattttgaga cggcatattt
     2281 tctatacaaa cttgaggaga acatgtcaat gactctgaaa gttgagcatt acattgcaga
     2341 tataacagaa taatgaatac ggtgttcaag tgagcatcgt ttttcaaacc tagttcagat
     2401 acaaaaaagt atcaaacaaa aaaaaataaa aaaacattat aaaaacaatt gacaagctaa
     2461 ttttgaagtg ataaaataaa tgcaagaaaa caaaaggaga ggtatatgag aagtttgttt
     2521 aagaaaatag ttgctgtatt agttattggc ttaattctgt taggaattgc gagaacacct
     2581 caagttcata agatggctag agggattgat cctggtcctg cgaattttat ttagaaatca
     2641 atttctacta attgagtact aattgagtat taatgggtaa aaagattgta tggataagaa
     2701 aaatttactt gtattggtta ctggttcggt aggtgccagt aatgtagata catacttata
     2761 ttatataaaa aaattctata atgtta
//
LOCUS       DAVKMU010000103         2655 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKMU010000103 DAVKMU010000000
VERSION     DAVKMU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.103"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..679
                     /locus_tag="VW343_002071"
     CDS             <1..679
                     /locus_tag="VW343_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9756557.1"
                     /translation="EVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPC
                     EIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPKTV
                     HSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINC
                     HFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFF
                     AHIDALI"
     gene            736..1533
                     /gene="psrA"
                     /locus_tag="VW343_002072"
     CDS             736..1533
                     /gene="psrA"
                     /locus_tag="VW343_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9756558.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1544..2143
                     /locus_tag="VW343_002073"
     CDS             1544..2143
                     /locus_tag="VW343_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9756559.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKALTKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQL"
     gene            complement(2097..>2655)
                     /locus_tag="VW343_002074"
     CDS             complement(2097..>2655)
                     /locus_tag="VW343_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9756560.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIIFKVDLLFQKVSQLFE"
BASE COUNT          916 a          380 c          459 g          900 t
ORIGIN      
        1 cgaagtttta cttgaaaaaa tacgagcaga aaaacaaaaa ctctttgaag aaggcaagat
       61 taaaaagaaa gatttggaca tttctattgt ttcccaagga gatgataact cttattatga
      121 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
      181 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      241 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccaa
      301 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
      361 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgg
      421 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcggggg ttattaattg
      481 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      541 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      601 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      661 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      721 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      781 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      841 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      901 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      961 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
     1021 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
     1081 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1141 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1201 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1261 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1321 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1381 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1441 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1501 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1561 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
     1621 aaaaataaaa gatatttttt caatgaatac aggtctttct tacaagaagg gcgatttaag
     1681 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
     1741 tctgttggat aacgattact acattgatac acaattcatc tcctctgagc aagtttattt
     1801 aaaacataat cagctaataa cacctgtatc aacctcttta gaacatattg gaaagtttgc
     1861 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
     1921 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtcct ctccgttatt
     1981 ttataaacaa ttgaaagcat taactaaact atcaggtcaa gctttatata atattcctaa
     2041 aactacactg agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
     2101 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tgaaaatgat tcttttcatc
     2161 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
     2221 cctttgatag catcttttga atcacctaaa gtagagataa gtggcaaaaa tatcattaag
     2281 taatctctga taatattttc tttattagca taggggaata tcgatataat ggcttcatta
     2341 tgagtggcag gaatatccaa tatggcaact tttccaatag ataatttaaa actcattaat
     2401 aaagttcctt taggtgaaat gtctattttc tttgatttta atgctaattt agaaatagat
     2461 tctctcgtat tagttacata accagatata ggcatatctg atatagatac ccaaggtatt
     2521 tcagttcccc aaaaagtagc ttcactgcgt ggaggagttt ttcctattct gaagttaact
     2581 aggctagcaa atttaatata tctccatgct tctgggattt catatatagg ataagaggtt
     2641 gtttcgtctt tgttc
//
LOCUS       DAVKMU010000104         2583 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKMU010000104 DAVKMU010000000
VERSION     DAVKMU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.104"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..438)
                     /locus_tag="VW343_002075"
     CDS             complement(<1..438)
                     /locus_tag="VW343_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756561.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYPLKRAI"
     gene            complement(452..1165)
                     /locus_tag="VW343_002076"
     CDS             complement(452..1165)
                     /locus_tag="VW343_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9756562.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1625..2005)
                     /locus_tag="VW343_002077"
     CDS             complement(1625..2005)
                     /locus_tag="VW343_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9756563.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            complement(2422..2574)
                     /locus_tag="VW343_002078"
     CDS             complement(2422..2574)
                     /locus_tag="VW343_002078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756564.1"
                     /translation="MLEKVTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLA
                     LLKESK"
BASE COUNT          838 a          504 c          393 g          848 t
ORIGIN      
        1 aatcgctctt tttaaaggat aatactcttt aatattatta actttttgtt cgtaggcata
       61 ttttgctaca tgatctaggt atatatcaaa aaagtcatga ggaaaatctc ccatacagcc
      121 attacgattc atattgaggc tatattttcg attagtaaga taattcggaa atatatttcc
      181 tattactcta agattatcta cttgtgaatc ccttctccca atataatccc cttctttgac
      241 taatgatgga gatttccaag taaaaattaa atcgccagag aaatttttct tatcaaaaaa
      301 cttaaaaaga gattgataaa tactatttgc taattgacaa gtgtcagatg taaattcata
      361 attaacagct tcattaaaat caacttcaga catttcaatg tactcttcaa tagtaatatt
      421 agcttttttt gtagtcatta gctaatctcc tttaaaatgt gatcttttca agaatatttt
      481 tatctaaaca atccagcaag tcttggtaag aatagacttc gtaagtcggc tgggcttgtg
      541 tgtgattttc gaggtgatga ggattatacc agatagtgtc aatccccgca ttattgccac
      601 cttgaatgtc ggcggttaga gaatctccaa tcatcagcgt cttttcttta ctaaatccag
      661 caatttgctg gccaatcttt tcataaaaga gagcatccgg cttttgagtt tgcaactgtt
      721 ctgagataaa gacttgattg aaataaggtg ctagaccaga ttgagccaaa cgtcctgtct
      781 gaatggcagt aatgccattt gtcgcagcat acaagtcata atcacgctca atgaggctgt
      841 ccaagagatc atgagcgccc gatagtgttt gtccctgctg ggcgaggtaa aattggtaac
      901 gctgggcaag aaaactaccg tctttttcct gtccaaaatg agaaaataaa cgagaaaagc
      961 gcgtgttaac cagctcttgt ttactgattt tcttcagctc caagtctttc cagagagcct
     1021 tgttcatagg aacgtaataa tctttataag cctgaatatc cgcaactcct tcttctttta
     1081 gaagttgggt caaagccaca tcctcagcag catcaaaatc aagaagagtg tggtcgaggt
     1141 cgaagagtag aaatttgtag aacaatttga ggttttcctt tctgaaaatt cattaaaaac
     1201 attatatcat aaagcacctc atacaattaa ctaatttaat cacttaaaaa aaattcgaac
     1261 actttctata caactgacag ctcaaatctt tcagaataga acaatactaa ctatcgagca
     1321 cccccgtctt cataaatata tgtgtaattc tagacctaga attcctataa actaaatgct
     1381 ttcatactct tccaagtaat tgattgcctt aaattttaat ttttgaagga ttctaaagct
     1441 agaatagccc catcacaatc agttttgatt gattcacaat ttagaaacac tatagtttca
     1501 ctcctgttaa aataaaaagg aactgcataa agcaatccct ttctaatttt gaaatcattt
     1561 acttaacatt ttatagttga gataatcaat agcttaccta taaaaagagt tatagtaaaa
     1621 ttccttattt attgatttca agctccgcta actgtgtttg aataactgac agttctgcac
     1681 cagcctgcag aagagcagct gcattatagg caccttctac aattggaacc ctgttgatga
     1741 tgatactttt atcactgaaa tcagtcacca tttttaagtt cattttagca gaacctaggt
     1801 caaaaaaggc aagtaaagta tctgctggat tttcggaaac aaccctatct acttgatcaa
     1861 aactcgttcc aattcctccg ccctcggttc ctcctacata agtaatcgga acatctttag
     1921 ctactttact aatcagttca acaacacctt ctgcaatgtg tttggaatgt gaaacgataa
     1981 caagaccaat accaatactt tccatcaaac caccccagtt tctaaaatag cagtaaagag
     2041 taatcctgat gagaatgatc caggatcaat atgtccaaga aaccatatgc tcctaagaca
     2101 agagccaaca gactggtcat caataatagt attgttcttt ttttcatcat tactccttaa
     2161 ctagtgttta actgattaat tagccagtaa atagtttatc tttatttaca ctatctgtta
     2221 agatatagta aaataagaac aggacagtca aatcgatttc taacaatgtt ttagaagtag
     2281 aggtatacta ttctagtttc aatctactat attttgcaca ttttcatgaa aaaaatgaga
     2341 actagaactc acattctgct ctcatttttc gttttcccgt tctcctatcc tgtttttagg
     2401 agttagaaaa tgctgctacc tttacttact ctcctttaat aaagccaaga gactatctac
     2461 ttctgctgaa atagtgttat tatctttcgt tccgagaaga agactacttt ttagaccagt
     2521 caatgtctcc acagcatttt gtctaatact agaatctgtt actttttcta gcaaggcctc
     2581 cgc
//
LOCUS       DAVKMU010000105         2539 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKMU010000105 DAVKMU010000000
VERSION     DAVKMU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.105"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..2367
                     /locus_tag="VW343_002079"
     rRNA            <1..2367
                     /locus_tag="VW343_002079"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2444..>2539
                     /gene="rrf"
                     /locus_tag="VW343_002080"
     rRNA            2444..>2539
                     /gene="rrf"
                     /locus_tag="VW343_002080"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          705 a          513 c          747 g          574 t
ORIGIN      
        1 agaacctgaa accgtgtgcc tacaacaagt tcgagcccgt taatgggtga gagcgtgcct
       61 tttgtagaat gaaccggcga gttacgttat gatgcgaggt taagttgaag agacggagcc
      121 gtagggaaac cgagtctgaa tagggcgcct tagtatcatg acgtagaccc gaaaccatgt
      181 gacctaccca tgagcaggtt gaaggtgcgg taagacgcac tggaggaccg aaccagggca
      241 cgttgaaaag tgcttggatg acttgtgggt agcggagaaa ttccaaacga acttggagat
      301 agctggttct ctccgaaata gctttagggc tagcgtcgac attagagatt cttggaggta
      361 gagcactgtt tgggtgaggg gtccatcccg gattaccaat ctcagataaa ctccgaatgc
      421 caatgaatta tggtcggcag tcagactgcg agtgctaaga tccgtagtcg aaagggaaac
      481 agcccagacc accagctaag gtcccaaaat aattgttaag tggaaaagga tgtggggttg
      541 cacagacaac taggatgtta gcttagaagc agctattcat tcaaagagtg cgtaatagct
      601 cactagtcga gtgaccctgc gccgaaaatg taccggggct aaaacaattt accgaagctg
      661 tggatacctt tataggtatg gtaggagagc gttctatgtg tgatgaaggt ataccgtgag
      721 gagtgctgga acgcatagaa gtgagaatgc cggtatgagt agcgaaagac aggtgagaat
      781 cctgtccacc gtaagactaa ggtttccagg ggaaggctcg tccgccctgg gttagtcggg
      841 acctaaggag agaccgaaag gtgtatccga tggacaacag gttgatattc ctgtactaga
      901 gtatgtagtg atggagggac gcagtaggct aactaaagca gacgattgga agagtctgtc
      961 taagcagtga ggtgtgaatt gagtcaaatg cttaattcta taacattgag ctgtgatggg
     1021 gagcgaagtt tagtagcgaa gttagtgacg tcacactgcc aagaaaagct tctagcgttt
     1081 aaacatactc tacccgtacc gcaaaccgac acaggtagtc gaggcgagta gcctcaggtg
     1141 agcgagagaa ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg
     1201 tgctgacttt aagtcagccg cagtgaatag gcccaagcaa ctgtttatca aaaacacagc
     1261 tctctgctaa atcgtaagat gatgtatagg gggtgacgcc tgcccggtgc tggaaggtta
     1321 agaggagtgc ttagcgtaag cgaaggtatg aattgaagcc ccagtaaacg gcggccgtaa
     1381 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg
     1441 cgtaatgatt tgggcactgt ctcaacgaga gactcggtga aattttagta cctgtgaaga
     1501 tgcaggttac ccgcgacagg acggaaagac cccatggagc tttactgcag tttgatattg
     1561 agtgtctgta ccacatgtac aggataggta ggagtctaag agatcgggac gccagtttcg
     1621 aaggagacgc tgttgggata ctacccttgt gttatggcca ctctaaccca gataggtgat
     1681 ccctatcgga gacagtgtct gacgggcagt ttgactgggg cggtcgcctc ctaaaaggta
     1741 acggaggcgc ccaaaggttc cctcagaatg gttggaaatc attcgcagag tgtaaaggta
     1801 taagggagct tgactgcgag agctacaact cgagcaggga cgaaagtcgg gcttagtgat
     1861 ccggtggttc cgtatggaag ggccatcgct caacggataa aagctaccct ggggataaca
     1921 ggcttatctc ccccaagagt tcacatcgac ggggaggttt ggcacctcga tgtcggctcg
     1981 tcgcatcctg gggctgtagt cggtcccaag ggttgggctg ttcgcccatt aaagcggcac
     2041 gcgagctggg ttcagaacgt cgtgagacag ttcggtccct atccgtcgcg ggcgtaggaa
     2101 atttgagagg atctgctcct agtacgagag gaccagagtg gacttaccgc tggtgtacca
     2161 gttgtcttgc caaaggcatc gctgggtagc tatgtaggga agggataaac gctgaaagca
     2221 tctaagtgtg aaacccacct caagatgaga tttcccatga ttatatatca gtaagagccc
     2281 tgagagatga tcaggtagat aggttagaag tggaagtgtg gcgacacatg tagcggacta
     2341 atactaatag ctcgaggact tatccaaagt aactgagaat atgaaagcgt attgttttct
     2401 tggtttgaat agatattcaa ttttgagtag gtattactca gagttaagtg acgatagcct
     2461 aggagataca cctgtaccca tgccgaacac agaagttaag ccctagaacg ccggaagtag
     2521 ttgggggttg ccccctgtg
//
LOCUS       DAVKMU010000106         2430 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKMU010000106 DAVKMU010000000
VERSION     DAVKMU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.106"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            145..1690
                     /locus_tag="VW343_002081"
     rRNA            145..1690
                     /locus_tag="VW343_002081"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1742..1814
                     /locus_tag="VW343_002082"
     tRNA            1742..1814
                     /locus_tag="VW343_002082"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1775..1777,aa:Ala,seq:tgc)
     gene            1939..>2430
                     /locus_tag="VW343_002083"
     rRNA            1939..>2430
                     /locus_tag="VW343_002083"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          685 a          502 c          712 g          531 t
ORIGIN      
        1 tcgcttgaga gaagcaagtg acaaagacct ttgaaaactg aacaagacga accaatgtgc
       61 agggcgctac aacataagtt gtagtactga acaatgaaaa aacaataaat ctgtcagtga
      121 cagaaatgag tgagaactca aacttttaat gagagtttga tcctggctca ggacgaacgc
      181 tggcggcgtg cctaatacat gcaagtagaa cgctgaagga ggagcttgct tctctggatg
      241 agttgcgaac gggtgagtaa cgcgtaggta acctgcctgg tagcggggga taactattgg
      301 aaacgatagc taataccgca taagagtaga tgttgcatga catttgctta aaaggtgcac
      361 ttgcatcact accagatgga cctgcgttgt attagctagt tggtggggta agggctcacc
      421 aaggcgacga tacatagccg acctgagagg gtgatcggcc acactgggac tgagacacgg
      481 cccagactcc tacgggaggc agcagtaggg aatcttcggc aatggacgga agtctgaccg
      541 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa gctctgttgt aagagaagaa
      601 cgagtgtgag agtggaaagt tcacactgtg acggtatctt accagaaagg gacggctaac
      661 tacgtgccag cagccgcggt aatacgtagg tcccgagcgt tgtccggatt tattgggcgt
      721 aaagcgagcg caggcggtta gataagtctg aagttaaagg ctgtggctta accatagtag
      781 gctttggaaa ctgtttaact tgagtgcaag aggggagagt ggaattccat gtgtagcggt
      841 gaaatgcgta gatatatgga ggaacaccgg tggcgaaagc ggctctctgg cttgtaactg
      901 acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgctg
      961 taaacgatga gtgctaggtg ttagaccctt tccggggttt agtgccgtag ctaacgcatt
     1021 aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc
     1081 cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct
     1141 tgacatccct ctgaccgctc tagagataga gttttccttc gggacagagg tgacaggtgg
     1201 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
     1261 cccctattgt tagttgccat catttagttg ggcactctag cgagactgcc ggtaataaac
     1321 cggaggaagg tggggatgac gtcaaatcat catgcccctt atgacctggg ctacacacgt
     1381 gctacaatgg ctggtacaac gagtcgcaag ccggtgacgg caagctaatc tcttaaagcc
     1441 agtctcagtt cggattgtag gctgcaactc gcctacatga agtcggaatc gctagtaatc
     1501 gcggatcagc acgccgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc
     1561 acgagagttt gtaacacccg aagtcggtga ggtaaccgta aggagccagc cgcctaaggt
     1621 gggatagatg attggggtga agtcgtaaca aggtagccgt atcggaaggt gcggctggat
     1681 cacctccttt ctaaggataa ggaactgcgc attggtcttg tttagtcttg agaggtcttg
     1741 tggggcctta gctcagctgg gagagcgcct gctttgcacg caggaggtca gcggttcgat
     1801 cccgctaggc tccattggtg agagatcacc aagtaatgca cattgaaaat tgaatatcta
     1861 tatcaaatag taacaagaaa ataaaccgaa aacgctgtag tattaataag agtttatgac
     1921 tgaaaggtca gaaaataagg ttaagttaat aagggcgcac ggtggatgcc ttggcactag
     1981 gagccgacga aggacgtgac aaacgacgat atgccttggg tagctgtaag taagcgatga
     2041 tccagggatt tccgaatggg ggaacccaac aggtagtacc tgttacccac atctgttaag
     2101 gatgtgagga ggaagacgca gtgaactgaa acatctaagt agctgcagga agagaaagca
     2161 aaagcgattg ccttagtagc ggcgagcgaa acggcagaag ggcaaaccga agagtttact
     2221 cttcggggtt gtaggactgc aatgtggact caaagattat agaagaatga tttgggaaga
     2281 tcagccaaag agagtaatag cctcgtattt aaaatagtct ttgtacctag cagtatcctg
     2341 agtacggcgg gacacgtgaa atcccgtcgg aatctgggag gaccatctcc caaccctaaa
     2401 tactccctag tgaccgatag tgaaccagta
//
LOCUS       DAVKMU010000107         2410 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKMU010000107 DAVKMU010000000
VERSION     DAVKMU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.107"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            301..1572
                     /locus_tag="VW343_002084"
     CDS             301..1572
                     /locus_tag="VW343_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9756565.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(1592..2407)
                     /locus_tag="VW343_002085"
     CDS             complement(1592..2407)
                     /locus_tag="VW343_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9756566.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
BASE COUNT          751 a          550 c          428 g          681 t
ORIGIN      
        1 acgatttcaa gcattttcct aaaaatatgt acaagtaata tgtacaagta ataaggggaa
       61 attagcataa aaacttttta aaacaaatca accccagata aaaatacaca tgttgtttta
      121 aagacaaaaa agggtacgaa aaactgtcac atctgcattt gattgctctt tgatttcaaa
      181 aaagtattaa aaatgggagt ataccacatt tgcccctaga tgtgaacgag agaaacgctc
      241 tagacattgc caagaaggaa aaaaaagggt acaatgtaac aaaatcaagg gaggtctgga
      301 atgaagaaac aaagcaagta caaagaggtc gtttcctatc tgaaaaatgg tatcgagtct
      361 ggacgatttc cgacgggtag tcgcctgcct tctatccgtc aactgagcct tgactttcac
      421 tgcagcaagg acaccattca acgagccctg ctggaattac ggcacgaaca atatctctat
      481 gccaagcctc agagtggcta ctatgtatta gaacaagggc aacatcaaga cctagaaatc
      541 gaggttaccg acgaacatgc cagtgcctat gacgatttcc gactctgtgt caatgaaacc
      601 ttgattggcc gagaaaacta cctcttcaac tactatgaca atcaagaagg attagaagac
      661 ctaagacagt ccattcacaa actcctcttt gagcaagctc tctactgcaa ggctaaccaa
      721 ctagtactga cttctggaac ccaacaagcc ttgtttatcc tctctcaaat atcctttcct
      781 agacaagcca aggaaatctt ggtggaacag ccaacctacc atcggatgaa tcgcctcttg
      841 attgcacagg ggctggacta tcaaacgatt gaacgaggca ttgatgggat tgacttggag
      901 gagctggaag gccacttcaa aacaggaaaa attaaatttt tctacaccat tcctagattt
      961 cactatcctc tgggacattc ctattctgag caggacaaac gatctattct taacttagct
     1021 gccaagtatg atgtctatat cgtagaggac gattatctgg gtgatttgga ctccaagaag
     1081 ggccaaacct tccactatct tgatacagag gagcgtgtca tttatatcaa gtccttctcg
     1141 accagccttt ttcctgccct tcgtattaca gcactcattc ttccaaatgc tatcaaagaa
     1201 gcatttgtgg cctacaaaaa tatcctagac tacgacagca acctcattat gcaaaaggcc
     1261 ctgtcactct atattgacag tcaattgttt gaaaaaaatc gtttggctcg cttgaccaat
     1321 catgaatctt accaaaaaca aatcgaggaa aggataacta aaacaccttg tccccttcct
     1381 cattatcccc tacacgatgg tttattgcta gacctgagac agtatcctaa aatcgccagt
     1441 ctcaaacaca gtcaactggg cttggacttc tttgaagagg cctatttaag cacctgtcct
     1501 tatcaatttg ccaaggtgtc cctagacaat ctggaaaatg ttttaaacta tttaaaagca
     1561 gaattggaat gacatccaac tctgcttttt cttattcttc aacttcagtt aaacttgctg
     1621 ctttttcaag atagctttgg taagttcctt catccttaag tttttggatg accttgtcta
     1681 ccacttcttt caaatcatca ctattttttc taagagcaac ggcattggcg tcgccgtcct
     1741 tcatcttcaa gctgacagtt gcgacagcta agccagcgtt tttagcagca taactaagtg
     1801 caacaggctc atccatatga acagcatcta cttttccagc ctgcaattca ttgactgctt
     1861 cacccatatt agttagggaa gttaattgag cttttggcaa ttgttccttg accattgatt
     1921 ctggaacagt ccctttttgg gctgcaatat tagcactttc taggctagtt aaatccttgt
     1981 atttttccac atcagcctta cgaaccaaga aactaatctt gttttcatag tatgggattg
     2041 aaaaatcaaa gacttctttt ctctcgtcag tagcactaat tcctgcaact gctaggtcag
     2101 ccttaccagt ttgaagactg gtcaaaacat tatcaaaact catgcttgag atttccaact
     2161 taaccccaag ttcatcagcg atagcctgag ccatgtcgat gtctgcaccg actacctggt
     2221 tctttccatc aaccaatgat tgaaattcaa agggtgcata gtcaggactg gttgccacaa
     2281 ctaatttccc cttttgctta atggcctcaa cagctgactg agaaccatta gaacctgact
     2341 ggcaagctac taagaagaaa cttgcaagaa aattacataa aacaaatatc catttttttg
     2401 atttcataaa
//
LOCUS       DAVKMU010000108         2309 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKMU010000108 DAVKMU010000000
VERSION     DAVKMU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.108"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            216..947
                     /locus_tag="VW343_002086"
     CDS             216..947
                     /locus_tag="VW343_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9756567.1"
                     /translation="MVNNVAVKVSNLSKEFLLGQDKTVSILKDISLSVNYGEFVSILG
                     VSGSGKSTLLSCLSSLSEPTSGEVVINGVNPYTLKEGKLAKFRRQDIAIIFQNYNLVP
                     ALPVLENVTLPLRLSGKSVDSNKVKKMLDSLNFKAELSSLVATLSGGEQQKVAITRAI
                     IADSKIIFADEPTGALDSVSRKLIFETLRNLASQGKCVLMVTHDIELASKTDRALILK
                     DGKISRQIIKPSADELYQALESSKD"
     gene            965..2281
                     /locus_tag="VW343_002087"
     CDS             965..2281
                     /locus_tag="VW343_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9756568.1"
                     /translation="MLKLIIYQFQYSKRQWLGTIPLLFVSSLIVGTSLFGIASSIKTA
                     NINASQLFQMLIIFGGTTLFFLISNNIRLLIDIFKKDYQLWTILGASRTQLSLLVSGQ
                     FYLMAVIVSSIGTILSFIMADSYYKFLQNLLGRDELPDLVITANIQSILLSIFIVPTI
                     VGIGAYFYSSRILKISSILKPKKKKRKVTVTGFVNISVRLFLWLLCIGSIVSAGFIRN
                     KEIIEKQSSIILFLLIIHILIIQSLSPSIQMFLIKFLMRIFPTENYVINTGFWNLLSN
                     PSYLKSIQTSMSMGVTLISGFILYTQNMYSFMNTANGVNEARASFIAYMSAPIILIIT
                     SSISLTILSSNKDIEDIKQLKTLGVSRLQLFKIRIGEAIIHSVLILLVSVIFNLIILI
                     LVSIIGQFLGRSLVDISGFWQPSLIVISLLVIFYSITKGFYLFQDR"
BASE COUNT          798 a          331 c          364 g          816 t
ORIGIN      
        1 aagctatgtt tacgctttca atcaagtctc atttatgata ctattatggt aatagaaaaa
       61 attcttagtt cgctaaagac ctttattcga gatccagagg aaatagaaat tttatccatt
      121 aaaaatacgt ttcataagtt acttacttga tattaataga attccaatat tgtataatta
      181 aatcaaagat gacactggaa tcaggaggat ttattatggt taataatgta gctgttaaag
      241 tctcaaacct ttcaaaagaa tttttgttag ggcaagacaa aactgtctct attttgaaag
      301 atatttcctt atctgtcaat tatggggaat ttgtatcaat acttggtgtc agtggttctg
      361 gaaagtctac tttgctaagt tgcttatcaa gtttatctga accaacaagt ggcgaagttg
      421 ttatcaatgg tgtaaatcca tataccttaa aagaagggaa gcttgctaaa tttagaagac
      481 aagatattgc aataattttt caaaactata atctggtacc tgctctacct gtactagaaa
      541 atgttacact tcctctgaga ctatcaggaa agagtgttga tagcaataaa gtaaaaaaaa
      601 tgttggatag tttgaatttt aaagcagaac tatcatcatt agttgctacc ttatcaggtg
      661 gagaacagca aaaagtggct attactcgtg caataatagc tgatagtaaa attatttttg
      721 ctgatgagcc aacaggagct ctggacagcg tctcaagaaa acttatattt gaaacacttc
      781 gtaatttagc gagtcaagga aaatgtgttc ttatggtcac tcatgatatt gagttggctt
      841 caaaaactga cagagcactc attttaaaag acggaaaaat atctcgacaa attattaaac
      901 cttcagctga tgagctctac caagcacttg aaagtagtaa agattaattt gaggaggatt
      961 tattatgcta aaattaatca tttaccaatt tcagtactca aaaagacaat ggttaggaac
     1021 aataccatta ctatttgtat ctagcctgat tgttggaaca tctttatttg gtattgctag
     1081 ttcaatcaag actgctaata ttaacgcttc acaacttttt caaatgctaa taatttttgg
     1141 cgggactact ctatttttcc ttatttcaaa taatataaga ctgctaatag atatctttaa
     1201 aaaggattac caattatgga ctatcttagg cgcaagcaga actcagctat ctttattagt
     1261 atctggacag ttttatttaa tggcagtgat tgttagtagt attggtacaa tactttcatt
     1321 tatcatggca gatagttatt acaaattttt acaaaattta ttaggaagag atgagttacc
     1381 tgatttagtt attacagcca acattcaatc aattttattg agtattttta tagtcccaac
     1441 aattgtcggg ataggggctt acttttattc aagtcgaata cttaaaatat catcaatatt
     1501 aaaaccaaaa aagaaaaaaa gaaaagttac agtaactgga tttgttaaca tctctgttcg
     1561 tttattccta tggctactat gtataggttc tattgtttca gcaggtttta tcagaaacaa
     1621 agaaataatt gaaaaacagt caagcataat attattttta ttaattattc acatccttat
     1681 tattcaatca ctatctccat caattcaaat gtttctaata aaatttttaa tgaggatatt
     1741 tccaactgaa aattatgtaa ttaatacagg cttttggaat ctactatcca atcctagcta
     1801 tttgaaaagt atacaaactt caatgagcat gggagtaact ctcatttctg ggtttattct
     1861 ttacacccaa aatatgtatt cattcatgaa tacagcaaac ggagtaaacg aagcaagagc
     1921 ttcctttatt gcttatatgt ctgcaccaat tattctgatt attactagtt ctatttctct
     1981 tacaatttta tcttctaata aagatattga ggacattaaa cagttaaaga cactgggagt
     2041 gtcaagatta caacttttta aaatacgtat aggcgaggca ataatacatt cggtgttaat
     2101 tttattagtg tcagtaattt tcaatttaat tattttaatt ttagttagca ttattggcca
     2161 atttttaggt cgtagtttag tagatatatc aggtttctgg caaccgagtc ttatagttat
     2221 ttctttgtta gttatttttt atagtattac aaagggattt tatttatttc aagatagata
     2281 aattggaata gttactgaac caaaaagaa
//
LOCUS       DAVKMU010000109         2209 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKMU010000109 DAVKMU010000000
VERSION     DAVKMU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.109"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            72..523
                     /locus_tag="VW343_002088"
                     /pseudo
     CDS             72..523
                     /locus_tag="VW343_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            688..987
                     /locus_tag="VW343_002089"
     CDS             688..987
                     /locus_tag="VW343_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9756569.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1096..2209)
                     /locus_tag="VW343_002090"
                     /pseudo
     CDS             complement(1096..2209)
                     /locus_tag="VW343_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          680 a          426 c          379 g          724 t
ORIGIN      
        1 taccacattg tgtactattt ttggttcatt tcactataat aataacgata ctagataatg
       61 aggaaaatgc aatggcaaac ccaactttcg gagaaaaaaa gtgaatacag actatattac
      121 tcgctatggc gtatatgcag ttatccctaa tcctgaacaa aaacaaattg ttcttgttca
      181 agaaccaaat ggtgcttggt tcctaccatg tggaaaaatt gaagcaggtg aaaatcatca
      241 ggaagcccta aagcgtgagt tgattgaaga gcttggtttc acagcagaaa ttggtaccta
      301 ttacggacaa gctgacgaat atttctattc tcgtcatcgt gatacctact actacaatcc
      361 tgcctacctc tatgaagcaa ctcctttcaa agaagtacaa aagccactag aaaactttaa
      421 tcatattgcc tggttcccta ttgacgaggc tatcaaaaac cttaaacgtg gtagccataa
      481 atgggccatt gaatcttgga aaaaacagca taagattggc taatactctt cgaaaataaa
      541 attcaaatca cgtaagcgtc gccttaccgt actcaagtat agcctgcggc tagcttccta
      601 gtttgctctt tgattttcat tgagtataaa tcaagcgact ttattcaaaa agtgttataa
      661 tagaattaga aaatcggagg aaatattatg aagcttatca atacgacaaa ctcacactcg
      721 caacttgtaa aaagtcaact agaaagtacc gatgcaaccc ttgttgaggt ttattctgca
      781 gggaatacag atgttatttt tacccaagct ccgcttcact atgaaatcct catctcaaac
      841 aaacaccgtg ctattcgtga gcctgaaatc gaaaccattc aagaattttt cttgaaacgt
      901 aaaattgata agacctctgt tgatgaagcc aatatcaaga cactctactc agagaaatta
      961 attggaattt caatcccaat caagtaagaa aattgagtaa aaactcgatt tgagagaaag
     1021 tccaataagt ctttccataa caaaacgcat agtagcaaga gttttgtact gaccccaaaa
     1081 agttagacaa ataatttatc cgaaggattt agttctgtat tgcacaaggc taagtccttt
     1141 tagttttacc ttaattcgtt tattgttgta gtaatcaata tagtctacaa tagcttgttc
     1201 caattgatta agtgatttaa atgttttctc atagccataa aacatttccg atttcagaat
     1261 cccaaagaag gactccatca taccgttgtc ttggctgttt cccttacgtg acatggatgc
     1321 ttgaattccc ttaccctcaa gaaactgatg ataagaatcg tgttgatatt gccagccttg
     1381 gtcactatgg agaatcgtat tctcgtagtg cttctctgtg aatgcctgtt ccaacattgt
     1441 ttgtacttgt tctaagttgg gtgaagttga aagattatag gcgataattt cgctattaaa
     1501 gccatctaaa actggtgata agtaaagctt ttgagtactt gctggaatgg caaattctgt
     1561 cacatctgta tagcactttc ccattgtttt agagccttca aattggcctt gaatgagatt
     1621 ctctgccttc ttgccaacgt ctcctttatg agaagaatat tttcgtttct gtcgcatttt
     1681 agcttgtaaa ttgagtactt tcatcaagcc ttgaactctt ttatgattta ccagataacc
     1741 acgatttctt agttctaaat aaatccgacg ataagcataa tttcccttgt gttcgataaa
     1801 aatggattga atttcagctt taagctcttg gtccttatct gttttgtcta gctgtttcaa
     1861 gtgatagtag taggtcgaac gagctagttt aatggatttt agaagaagaa ctaacgaaaa
     1921 ctcagtcatt aattcttgaa caatttctgt ctttcttctt tctctttttc ctccttcaat
     1981 cggagttctc ttaacttttt taggatggca ttctccgctc tcaggtactc tcccttttgt
     2041 tttctcaaca atagtatacc cgtttttcct gtattgtgct agccagttaa gaagtatcgt
     2101 acgacttggg aggccgtatt caagagaaac tctatcttta gtccagcctt catgtcagac
     2161 tttatgaatc atttcttgtt ttaaatcagg agaatagtaa cgatttttt
//
LOCUS       DAVKMU010000110         2190 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKMU010000110 DAVKMU010000000
VERSION     DAVKMU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.110"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..342)
                     /locus_tag="VW343_002091"
                     /pseudo
     CDS             complement(1..342)
                     /locus_tag="VW343_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(287..469)
                     /locus_tag="VW343_002092"
     CDS             complement(287..469)
                     /locus_tag="VW343_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9756570.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            804..>2190
                     /gene="pcpA"
                     /locus_tag="VW343_002093"
     CDS             804..>2190
                     /gene="pcpA"
                     /locus_tag="VW343_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9756571.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNRGV"
BASE COUNT          780 a          323 c          354 g          733 t
ORIGIN      
        1 cgtaacacca ctttgaacaa gagttacttc aacccattgg ctccgacgga ttaagttgct
       61 ttcgtggata ccaaaatcag ccgcaatttc ttcataagtg cggtattctc acacatattg
      121 aagagtggcc ataagaaggt cttctaggct taatttaggt tttcgtccac cttttgcgtg
      181 tttaagttga taagctgttt ttaatacagc taacatctct ttaaaagtcg tgcgctgaac
      241 accaacaaga cgcttaaatc gtgtatcagt taattgttta cttgcttcat aatttcgcag
      301 ggagtctatt gactctttgg taggtgtcaa tgtttttttc atctatcccg agaattattt
      361 tcccgccatt tgtatttgca aatgctgagt aggtttccca gaaagactct ggaagattgt
      421 ttttagcttt tttgtattct aaatcaaccc cttcaaattt taagtccata tttttccttt
      481 acatctgttt tttgtggttc tggtatttgt tcaagttgag tgataatata gcgaattgaa
      541 tttcgagagt ttttactcag ttaatttctt ttttaaccca ctttaatttc ttttttaaca
      601 cgggttaaaa aagaaattaa agtgggttaa ttttccttga cttaaaattt aaaaatgata
      661 tactgtgttt atggagagat gacaatttat aaaatgtact actctattct actagataat
      721 gattgaataa aactttttat aacaacggct agcaaagtta taattttata tctattagaa
      781 aataaatatg taaggaaatt taaatgaaaa aaactacaat attatcatta actacagctg
      841 cggttatttt agcagcatat gtccctaatg aaccaatcct agcagatact cctagttcgg
      901 aagtaatcaa agagactaaa gttggaagta ttattcaaca aaataatatc aaatataagg
      961 ttctaactgt agaaggtaac ataggaactg ttcaagtggg taatggagtt actcctgtag
     1021 agtttgaagc tggtcaagat ggaaaaccat tcacgattcc tacaaaaatc acagtaggtg
     1081 ataaagtatt taccgttact gaagtagcta gtcaagcttt tagttattat ccagatgaaa
     1141 caggtagaat tgtctactat cctagctcta ttactatccc atcaagcata aaaaaaatac
     1201 aaaaaaaagg cttccatgga agtaaagcta aaactattat ttttgacaaa ggcagtcagc
     1261 tggagaaaat tgaagataga gcttttgatt tttctgaatt agaagagatt gaattgcctg
     1321 catctctaga atatattgga acaagtgcat tttcttttag tcaaaaattg aaaaagctaa
     1381 ccttttcctc aagttcaaaa ttagaattaa tatcacatga ggcttttgct aatttatcaa
     1441 atttagagaa actaacatta ccaaaatcgg ttaaaacatt aggaagtaat ctatttagac
     1501 tcactactag cttaaaacat gttgatgttg aagaaggaaa tgaatcgttt gcctcagttg
     1561 atggtgtttt gttttcaaaa gataaaactc aattaattta ttatccaagt caaaaaaatg
     1621 acgaaagtta taaaacgcct aaagagacaa aagaacttgc atcatattcg tttaataaaa
     1681 attcttactt gaaaaaactc gaattgaatg aaggtttaga aaaaatcggt acttttgcat
     1741 ttgcggatgc gattaaactt gaagaaatta gcttaccaaa tagtttagaa actattgaac
     1801 gtttagcctt ttacggtaat ttagaattaa aagaacttat attaccagat aatgttaaaa
     1861 attttggtaa acacgttatg aacggtttac caaaattaaa aagtttaaca attggtaata
     1921 atatcaactc attgccgtcc ttcttcctaa gtggcgtctt agattcatta aaggaaattc
     1981 atattaagaa taaaagtaca gagttttctg tgaaaaaaga tacatttgca attcctgaaa
     2041 ctgttaagtt ctatgtaaca tcagaacata taaaagatgt tcttaaatca aatttatcta
     2101 ctagtaatga tatcattgtt gaaaaagtag ataatataaa acaagaaact gatgtagcta
     2161 aacctaaaaa gaattctaat cggggagtag
//
LOCUS       DAVKMU010000111         2137 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKMU010000111 DAVKMU010000000
VERSION     DAVKMU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.111"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..192
                     /locus_tag="VW343_002094"
     CDS             <1..192
                     /locus_tag="VW343_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756572.1"
                     /translation="IRSAYSGSDITPVYSLGSGSRIVMYYNGGGDNYIGSGTRLAMAP
                     QFGNHVRIHTSGSWSPDSY"
     gene            251..925
                     /locus_tag="VW343_002095"
     CDS             251..925
                     /locus_tag="VW343_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HEU9756573.1"
                     /translation="MLHPIFIIRRSWDGIFHLSEWKRNEEFDFFNKMDVPYLSMSSRI
                     EVTQAINFHKKHSISLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTS
                     EDKLNFSSFTMGDNLIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVST
                     PMNLSKIDTVPRITWGKVIQENQRYFCTVNLQINHGMGDGLHVSNFFVLLQRFVNKIN
                     EYFQKK"
     gene            993..>2137
                     /locus_tag="VW343_002096"
     CDS             993..>2137
                     /locus_tag="VW343_002096"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9756574.1"
                     /translation="MSIFIGGAWPYANGSLHIGHAAALLPGDILARYYRQKGEEVLYV
                     SGSDCNGTPISIRAKKENKSVKEIADFYHKEFKETFEKLGFTYDLYSRTDSPLHHEIV
                     QELFLQLYEKKFLYTKKIKQLYCTFDNQFLPDRFVEGKCPNCGTHSRGDQCDNCSAIL
                     DPIDLVDKRCSICSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETVRWRKNAINLTK
                     RYLREGLPDRAVTRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASVDWAQKLQNNIT
                     DFWNNRTKSYYVHGKDNIPFHTIIWPAILSGLEIEPLPEYIISSEYLTLENKKISTSN
                     NWAIWLNDIIKKYDADSIRYFLTINAPEMKDANFSWREFIYSHNSEL"
BASE COUNT          745 a          291 c          404 g          697 t
ORIGIN      
        1 attcgtagcg cctatagtgg gtcagatatt accccggtat attcattggg gtctggctct
       61 aggattgtca tgtactataa tggaggtggt gacaattata ttggttctgg tactagatta
      121 gctatggcgc cacaatttgg aaatcatgta agaattcata cttcaggttc ttggagtcca
      181 gattcttatt aacttacttg tcagagtaag ccttaaagat ggttgattgt gggtgtagca
      241 tgaaaaaaga atgctacacc ctatttttat tataaggagg agttgggatg gaatttttca
      301 tttatcagag tggaaaagga atgaagaatt tgactttttc aataaaatgg atgttcctta
      361 tttatctatg agttcacgaa tagaagtaac gcaggctatc aattttcata aaaaacattc
      421 gatatcactt tatgccataa tatcgtggtg tgttatgagt gcaataaatt ctattccaga
      481 attactaatg gataccgacg gaaagattgt ctggcaatac aatcaaagag gttgttcttt
      541 cactaccctt acatcggaag ataagcttaa tttttcttca tttacgatgg gagataattt
      601 aattgaattt gtttctgcgt tcaacataaa taaacagaag gcagaagaag ggcaaaaacc
      661 aaatattgac aagaataaca ttgcttatct ttcttgtgtt ccatggattg attttttaca
      721 tgtaagcact cctatgaatc tttcaaaaat tgatacagtt cctcgaataa cgtggggaaa
      781 agttattcaa gagaaccaaa gatatttttg tacagtgaat ttacaaatta atcatggaat
      841 gggagatgga ttacacgtat ctaatttttt tgtgttattg caaaggtttg taaataaaat
      901 aaatgaatat tttcaaaaaa agtaatgatt gaaaagatat tgcatttaaa taatgttata
      961 ataaaaataa agaatttaag gagaaataca atatgtcaat ttttattgga ggagcatggc
     1021 catatgcaaa cggttcgtta catattggtc acgcggcagc gcttttaccg ggggatattc
     1081 ttgcaagata ctatcgtcag aagggagagg aagttttata tgtttctgga agtgattgta
     1141 atggaacccc tatttctatc agagctaaaa aagaaaataa gtctgtgaaa gaaattgctg
     1201 atttttatca taaggaattt aaagaaactt ttgaaaaatt aggttttaca tatgacttgt
     1261 actcgcgaac agattctcca ctacatcatg agatagtaca agaattattt ttgcaattat
     1321 acgagaagaa atttttgtat acaaaaaaaa ttaaacaact ctattgtaca tttgataatc
     1381 aattcttacc tgataggttc gttgaaggaa aatgcccgaa ttgtggaacc cattcaagag
     1441 gagatcaatg tgataattgc tcagcaattt tagatcctat agatttagtt gataaaagat
     1501 gttctatttg ttccaatgaa ccagaagttc gtgagacaga acacttttat tatgttttta
     1561 gtgagtttca aaatttgtta gagacttact taaatgatgc tgaagaaaca gtgagatggc
     1621 gaaaaaatgc aataaatcta actaaaaggt atttacggga aggtttacct gatcgagctg
     1681 ttactcgtga tctaccaaat gggattccag ttcccattga tggttttaga gataaaaaaa
     1741 tatatgtctg gtttgaagcg gtagcaggtt actacacagc aagtgttgat tgggcgcaaa
     1801 aacttcaaaa taatattact gatttttgga acaatcggac aaagtcatat tatgttcatg
     1861 gaaaagataa catcccattt catactatta tttggccggc aatactctca ggtttagaaa
     1921 tagagcctct tccagagtat attatttcta gtgaatattt aacgttagaa aacaaaaaaa
     1981 tatcaacaag taataattgg gctatatggc ttaacgatat cataaaaaaa tatgatgctg
     2041 attcaatccg atacttccta acaataaatg ctcctgagat gaaagatgca aatttttctt
     2101 ggagagaatt tatttatagt cataactcag aattatt
//
LOCUS       DAVKMU010000112         1881 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKMU010000112 DAVKMU010000000
VERSION     DAVKMU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.112"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..426
                     /locus_tag="VW343_002097"
     CDS             <1..426
                     /locus_tag="VW343_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEU9756575.1"
                     /translation="SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVI
                     GKIANGIADDLVTATVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESK
                     KTYEVGLRKAIDSTCSMLQPQSLVKELIKLENIVLEEGH"
     gene            647..1753
                     /locus_tag="VW343_002098"
     CDS             647..1753
                     /locus_tag="VW343_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU9756576.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
BASE COUNT          687 a          228 c          327 g          639 t
ORIGIN      
        1 agtaaggaat taattagtta tttttgtgat aaagtttatg atgaaatatt tgttgaagag
       61 gtagttccgc acgtttttct gccatatgaa tctgacttac ttcttatttt accagctacg
      121 gcaaatgtga ttggcaaaat tgctaatggt attgctgatg atttagttac agcaactgtt
      181 ttaaacttta ataaaaaaat aattttttgt cccaatatga actctactat gtgggacaat
      241 cacatagttc aaagaaatgt atcaattcta aaggagttgg gacatatatt tttatttgag
      301 tctaaaaaaa catatgaggt aggattgcgt aaagcaatag attcaacatg ttcaatgtta
      361 caaccacagt cgttagtaaa agaacttatc aaattagaaa atattgtcct tgaagaggga
      421 cattaaaaac tactgagaat attaaggagg ggaaaaaatg gaaaattcat caatcgatgt
      481 agatatgctg ttggaagaat tgacacaaga agcaatggtc gttgttgctg ttgataagga
      541 ctgttaattt aaacttatgg caatatatga aaggttactg gatgttttaa attatgcagg
      601 cagtagcctt ttattatata caaatggata aagtaaggat aatacaatga ttaataaaaa
      661 aatacaacaa gttgttttgg aatcattaca gaattttttg aatgggaact tcatttcgcc
      721 ttgtgtagtc tatgattttg gcttgctgga aactgtactt gatgaattta aaaatcaaat
      781 tcctgtaaca ttcaattacc aactttttta tgccgttaaa gcaaattcaa atgagaagat
      841 acttgaattc ttagtagata aaattgatgg agttgatgtg gcgtcattat ctgaattaga
      901 tgtggctaaa aaatttttcc caccaactca aatttctgtt aatggtcccg cattttctta
      961 tgaaacttta tataatctga ttaaaaaaca atataaagtt gatattaact ttttggaaca
     1021 tcttcaacaa ttttccccaa aagaatctgt tggaataaga gtaacggagc cagatgaact
     1081 taataatcgt atgagtcgat ttggaataaa tatttgcagt gataattgga ctagtaattt
     1141 acaaaatcct ttaattacac gactgcattt tcattttgga gaaaaagatg ataaatttat
     1201 tgttaagtta gataaaatat tatttaagtt acaagaaatt aataaactta gagaggttag
     1261 agaaataaat cttggaggcg gttttatgaa attatttatg gaaaatcgtt tgaaagaatt
     1321 ttttctatca cttatggaaa tctataaaaa gtacgatatt gatagtactg tgactacaat
     1381 aatagaacca ggtagtgcaa ttacttcatt ttctgcctat atgattacta gcccagttaa
     1441 tgttagtgag gtgaatgagc agcaggttat cacgttagac acatcaatat acaccaatac
     1501 attatggttt gttccgcata ttattacaac gttaaattca agtagtaaag agcgttatag
     1561 tactattctc tatggtaata cctgttatga acatgacaag tataaaatga aagtttcgct
     1621 tccaaggtta actcaaaata gcagtatagt gttttttcct gtaggagctt atataaaaag
     1681 caatcattca aatttacatc gtaatgattt tatgcgggag gtatatttgt ggacaaaaaa
     1741 cttgacatat tagataaagt taaggaatat ttaggaaata aaactactca aattctggat
     1801 aatcaatata aagaattttt gaaacttaat gatataaggc gagcgtttgg tatttcagaa
     1861 aaagtattaa acaattcttt t
//
LOCUS       DAVKMU010000113         1855 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKMU010000113 DAVKMU010000000
VERSION     DAVKMU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.113"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            16..88
                     /locus_tag="VW343_002099"
     tRNA            16..88
                     /locus_tag="VW343_002099"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Asp,seq:gtc)
     gene            113..185
                     /locus_tag="VW343_002100"
     tRNA            113..185
                     /locus_tag="VW343_002100"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:146..148,aa:Lys,seq:ttt)
     gene            191..272
                     /locus_tag="VW343_002101"
     tRNA            191..272
                     /locus_tag="VW343_002101"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:225..227,aa:Leu,seq:tag)
     gene            291..363
                     /locus_tag="VW343_002102"
     tRNA            291..363
                     /locus_tag="VW343_002102"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:324..326,aa:Thr,seq:tgt)
     gene            386..457
                     /locus_tag="VW343_002103"
     tRNA            386..457
                     /locus_tag="VW343_002103"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:418..420,aa:Gly,seq:gcc)
     gene            465..550
                     /locus_tag="VW343_002104"
     tRNA            465..550
                     /locus_tag="VW343_002104"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:499..501,aa:Leu,seq:taa)
     gene            560..633
                     /locus_tag="VW343_002105"
     tRNA            560..633
                     /locus_tag="VW343_002105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:594..596,aa:Arg,seq:acg)
     gene            649..722
                     /locus_tag="VW343_002106"
     tRNA            649..722
                     /locus_tag="VW343_002106"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:683..685,aa:Pro,seq:tgg)
     gene            728..801
                     /locus_tag="VW343_002107"
     tRNA            728..801
                     /locus_tag="VW343_002107"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:762..764,aa:Met,seq:cat)
     gene            815..888
                     /locus_tag="VW343_002108"
     tRNA            815..888
                     /locus_tag="VW343_002108"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:849..851,aa:Ile,seq:cat)
     gene            899..988
                     /locus_tag="VW343_002109"
     tRNA            899..988
                     /locus_tag="VW343_002109"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:935..937,aa:Ser,seq:tga)
     gene            999..1072
                     /locus_tag="VW343_002110"
     tRNA            999..1072
                     /locus_tag="VW343_002110"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1033..1035,aa:Met,seq:cat)
     gene            1076..1148
                     /locus_tag="VW343_002111"
     tRNA            1076..1148
                     /locus_tag="VW343_002111"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1109..1111,aa:Phe,seq:gaa)
     gene            1168..1238
                     /locus_tag="VW343_002112"
     tRNA            1168..1238
                     /locus_tag="VW343_002112"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1200..1202,aa:Gly,seq:tcc)
     gene            1274..1347
                     /locus_tag="VW343_002113"
     tRNA            1274..1347
                     /locus_tag="VW343_002113"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1308..1310,aa:Ile,seq:gat)
     gene            1354..1441
                     /locus_tag="VW343_002114"
     tRNA            1354..1441
                     /locus_tag="VW343_002114"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1388..1390,aa:Ser,seq:gct)
     gene            complement(1574..>1855)
                     /locus_tag="VW343_002115"
     CDS             complement(1574..>1855)
                     /locus_tag="VW343_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9756577.1"
                     /translation="KEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYS
                     NAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT          437 a          386 c          490 g          542 t
ORIGIN      
        1 ccgttaactc ccaaaggtcc cgtagtgtag cggttatcac gtcgccctgt cacggcgaag
       61 atcgcgggtt cgattcccgt cgggaccgtt taaggtaacg caagttattt tagactcgtt
      121 agctcagttg gtagagcaat tgacttttaa tcaatgggtc actggttcga gcccagtacg
      181 ggtcatatat gcgggtttgg cggaattggc agacgcacca gatttaggat ctggcgctta
      241 acggcgtggg ggttcaagtc ccttaacccg cattaagata taacaaatga gccggcttag
      301 ctcagttggt agagcatctg atttgtaatc agagggtcgc gtgttcaagt catgtagccg
      361 gcatttttag atagaagaaa acagtgcgaa cgtagttcag tggtagaaca ccaccttgcc
      421 aaggtggggg tcgcgggttc gaatcccgtc gttcgcttag agaggccggg gtggcggaac
      481 tggcagacgc acaggactta aaatcctgcg attggtaacg atcgtaccgg ttcgattccg
      541 gtcctcggca taatatggag cacccttagc tcaactggat agagtacctg actacgaatc
      601 aggcggttag aggttcgact cctctagggt gcatttttct atttaactcg ggaagtagct
      661 cagcttggta gagtacttgg tttgggacca aggtgtcgca ggttcgaatc ctgtcttccc
      721 gattcatggc ggtgtagctc agctggctag agcgtccggt tcatacccgg gaggtcgggg
      781 gttcgatccc cttcgccgct ataatgatct tgtcggacct ttagctcagc tggttagagc
      841 tctcggctca taaccgagtg gtcgtaggtt caagtcctac aaggtccatt tgaatattgg
      901 aggattaccc aagtccggct gaagggaacg gtcttgaaaa ccgtcaggcg tgtaaaagcg
      961 tgcgtgggtt cgaatcccac atcctccttt tatattaacg cgggatggag cagctcggta
     1021 gctcgtcggg ctcataaccc gaaggtcgta ggttcaaatc ctgctcccgc aataaggctc
     1081 ggtagctcag ttggtagagc aatggattga agctccatgt gtcggcggtt cgattccgtc
     1141 tcgcgccatt ttattgatag tatttatgcg ggtgtagttt agtggtaaaa ctacagcctt
     1201 ccaagctgtt gtcgcgagtt cgattctcgt cacccgcttt gaactttgtt caaattacca
     1261 agttttttaa cttgggcgcg tagctcaggt ggttagagcg cacgcctgat aagcgtgagg
     1321 tcggtggttc gagtccactc gtgcccatta attggagaat tactcaagag gctgaagagg
     1381 acggtttgct aaatcgttag gtcgggtaac tggcgcaagg gttcgaatcc cttattctcc
     1441 gttttaatga gagtttgtaa ctctcttttt tatttttaga gacaataaag cttcagatcc
     1501 gtaaaactta attgaattac taatctgaag ttatttttag gctctttgtc aactgtagtg
     1561 ggttgaaaaa aagctaagct cgagaaagga caaattttgt cctttctttt ttgatgttca
     1621 aagcgataaa aatccttttt ttgaagtttt caaagttccg aaatccaaag gcgtttcgtt
     1681 tgataagttt gatgagatta tttgtcgctt ccagtttggc gttagaatag tgtagttgaa
     1741 gggcgttgac aatcttttct ttatctttga ggaaggtttt aaagacagtc tgaaaaatag
     1801 gatgaacctg cttcagattg tcctcaataa gcccgaaaaa tttctctggt tcctt
//
LOCUS       DAVKMU010000114         1781 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKMU010000114 DAVKMU010000000
VERSION     DAVKMU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.114"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            9..>1781
                     /locus_tag="VW343_002116"
     CDS             9..>1781
                     /locus_tag="VW343_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEU9756578.1"
                     /translation="MANNRKTETLGVSYLSTFIDKHELLQSYFESNDKTPVWDGEIHV
                     LKSPSEKKDEILGKVPVQIKTTRQKKDVLKSFSLDTRDLELYKPNGGVVLFVVWLNED
                     NGLRDIYYKSLPPLSIKNLLKKSKLKNKSTNRKKLSIEIFKLDEKKMYPMLVDFINNS
                     QKQYSFINVEGISVEDIPDDKTLKFYFYGQEKEEIFNYQEEHDLFIYYLDPITGIEIP
                     LENTIKIVETEEETDLIIKIGDYVFQDVKRHRFPDGSVQLHFGESFTMSFDIKKKQFK
                     FNYTRPDLLSKAIKCTQVFQELGKIGYFTLNGNKIELDERSIKDISSLDLEADIKGLL
                     KISNFMKKMGIQKDVDLSCFDKQSQRNLNILYSGLVLKKKVALNYNESKLLHLNIANI
                     HIITLYSFLSDKNGTMIDIFTETPWCREGETEDEDYLDISIFEVFEPNDWLKIDNCKI
                     DSVIASYQRLVDNKLKYEGADRTILKIVIAADMAEDKTKRELLLNWAQCLSDWNLKYS
                     KNCEMAIINDLQIKSRVRKLNSKETETLTNILVNSNDNYELCFGSSVLLKSKPQADLF
                     WNKLDNETKERYKDFPIYTLYMKLS"
BASE COUNT          685 a          221 c          308 g          567 t
ORIGIN      
        1 gtgcagattt ggccaataat aggaaaactg agacattagg tgtttcatat ctttcaacat
       61 ttatagataa gcatgagtta cttcaatctt attttgaaag taatgataaa actccagtat
      121 gggatggaga aattcatgtt cttaaatcac cgagtgagaa gaaagacgaa atattaggta
      181 aagtacctgt tcagattaaa actactagac agaaaaaaga tgtgttaaaa tccttctcat
      241 tagatactag agatttagaa ctatataaac caaatggtgg agtagtttta tttgtagttt
      301 ggttgaatga ggataatggt ttaagagata tttactataa gtcattacct cctctttcta
      361 taaagaattt gctcaaaaag agtaaactaa aaaataaatc tacaaatagg aagaaacttt
      421 ccatagagat ttttaaactg gatgagaaaa aaatgtatcc aatgttagtg gatttcataa
      481 acaatagtca gaagcagtat agtttcataa atgtcgaagg tatttcagtt gaggacattc
      541 ctgatgataa aactctaaag ttctacttct atggtcagga aaaagaagaa atttttaact
      601 atcaggaaga acatgatctc tttatttatt atttagatcc tataactggc atagaaatac
      661 ctcttgaaaa cacaataaag attgtagaaa ctgaagaaga aacggattta attataaaaa
      721 taggcgatta tgtttttcaa gacgtaaaaa gacatcgttt tcctgatggg agtgtacagc
      781 ttcattttgg tgaaagcttt acaatgtctt ttgacataaa gaagaaacaa ttcaaattta
      841 actatactag gccggatttg ctgtcaaaag ctataaagtg tactcaggta tttcaagagc
      901 taggaaaaat cggatatttt acccttaatg gtaataaaat agagttagat gaacggtcaa
      961 taaaggatat ttcttcactt gatttagagg ctgatataaa gggattgtta aaaatttcta
     1021 actttatgaa aaaaatgggt atacaaaaag atgttgattt atcttgcttt gataaacaat
     1081 ctcaaagaaa tctaaatatt ctttattcag gtcttgtctt aaaaaagaaa gttgctttaa
     1141 attataatga atcgaaatta cttcacctaa atattgctaa catccacatt ataactttat
     1201 atagtttttt gtcagataaa aatggaacta tgatagacat ttttactgaa actccttggt
     1261 gccgggaagg agaaactgaa gatgaagatt atttagacat ttcaattttt gaagtatttg
     1321 aaccaaacga ttggctaaaa attgataatt gtaaaattga ttcagttatt gcctcgtatc
     1381 aaagattggt agataataag ctgaaatatg aaggcgctga ccgtactatt ttaaaaatag
     1441 ttatcgctgc agacatggca gaagataaga ctaaaagaga gttgttactt aattgggcgc
     1501 aatgtctttc tgactggaac ttaaaatact caaaaaattg tgagatggct ataataaatg
     1561 accttcaaat aaaatctaga gttcgtaaat taaacagcaa agaaacagag acactaacta
     1621 atattttagt aaatagtaat gataactatg aactttgttt cggttcatca gtgctattaa
     1681 aatcgaaacc acaagctgat ttgttttgga ataaactgga taatgagaca aaagaaagat
     1741 ataaggactt tcctatttat actttgtata tgaaattatc g
//
LOCUS       DAVKMU010000115         1719 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKMU010000115 DAVKMU010000000
VERSION     DAVKMU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.115"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>1719
                     /locus_tag="VW343_002117"
     CDS             <1..>1719
                     /locus_tag="VW343_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9756579.1"
                     /translation="KQVAPLPEYTGPQASAIVEPEQVAPLPEYTGVQAGSIVEPEKVE
                     APKEYTGKIEQPSAEDTKPENGASSTNGESERPKDKIKEEKQVDKKLELRNVSNVELY
                     TVENNKYRHITAVDGALDSSLKYFMKVKSENFKDIMLPVTKIESTTKNNKEVYKIVAH
                     AENLIQHENNVISNDYTYYLPKTQQSETGVYTSFKNLVDAMNSDPNGTFHLGATMDAR
                     EVELPDDQESYVKNEFYGKLIGKNNGKYYAIYNLKKPLFKTLNTATIQNLSIKEANVS
                     SKEDAATISKEAKYNTLIDNVHSDGIIAGERGIGGLVSKVDNSRISNSSFTGRITNTY
                     DTTAGYEIGGLVGKLSGSLASIEKSIASIDIASNAKSGDQIVGGIAGVVEKSATIKYS
                     YVEGNVNNVRHFGKVGGVAGNLWDRDSQDVSKSGKLSYVLSDVNVTNGNAIAGYNFNG
                     IKTIETYSNKNNKVVNVVQEDDEVVTKDSDVQRGTVLDADKVKEKKVELVSKHSTKVE
                     DFDFTSRYNTNYNEVTGYQQSREQVYKNIEKLLPFYNRETIVKYGNLVEDNSDLFTKK
                     LLSVVPM"
BASE COUNT          678 a          232 c          339 g          470 t
ORIGIN      
        1 aaacaggtag caccgcttcc agaatataca ggaccacaag ccagcgctat tgttgaacca
       61 gagcaggtag caccactacc agaatacaca ggggtacaag ccggatcgat agtcgagcca
      121 gagaaagtag aagcaccgaa agaatatact ggtaaaatcg aacagcctag tgcagaagat
      181 actaaacctg aaaatggggc aagctctaca aatggggaat cggaacgtcc taaagataaa
      241 attaaagaag aaaaacaagt cgacaagaaa ctagaactta gaaatgtttc aaacgtggaa
      301 ttatatacgg tagaaaataa taaatatcga catattacgg ctgtagatgg tgctttagat
      361 agttcattga agtatttcat gaaggtaaaa tcagaaaatt ttaaagatat aatgttgcct
      421 gttactaaaa ttgaaagtac aacaaaaaac aataaagaag tatacaaaat tgtggctcat
      481 gcagaaaatc taattcaaca cgaaaacaat gttatttcga atgactatac atattattta
      541 ccaaaaactc agcaaagtga aactggcgtc tatacatcat ttaaaaattt agttgatgca
      601 atgaatagtg accctaatgg aacattccat ttaggtgcaa ctatggatgc tcgtgaagtt
      661 gaacttccgg atgatcaaga aagttatgta aaaaatgaat tttacggaaa attaataggt
      721 aaaaataatg gtaagtatta tgctatttat aacttgaaaa aacctttatt taaaactcta
      781 aacaccgcta ctattcagaa tctttcaata aaagaagcga atgtttcttc taaagaagat
      841 gctgctacaa tatcgaaaga agcaaaatac aatactctta tagataatgt tcattctgat
      901 ggtattatag ctggtgaacg tggaattggt ggattggttt caaaagtaga taactcgaga
      961 atttctaata gtagttttac aggcagaata actaacacat atgatacaac tgcagggtat
     1021 gaaattggtg gtttagtagg taaactttct ggttcgcttg cttctataga gaaatcaata
     1081 gcatcgattg acatagcctc taatgcaaaa tcaggagatc aaattgttgg tggtatagcc
     1141 ggagttgttg agaaaagtgc gacaattaag tatagctatg ttgaaggaaa tgttaacaat
     1201 gtgcgacact ttggtaaagt tgggggagtt gcaggtaatc tttgggatag agattcacaa
     1261 gatgtaagta aatcaggtaa actatcatat gttttaagtg atgtcaatgt tacgaatggt
     1321 aatgctatag ctggctataa tttcaatgga ataaaaacga ttgaaacata tagcaataaa
     1381 aataataagg tagtaaacgt agtacaagaa gatgatgagg ttgtgactaa agattcagat
     1441 gtacaaagag gtacggtatt agatgctgat aaagtaaaag agaaaaaggt agaattggtt
     1501 tctaaacaca gcactaaagt agaagatttt gattttactt caagatacaa tactaattac
     1561 aatgaagtaa caggttacca acagtcgaga gaacaagtat ataaaaatat tgaaaaatta
     1621 cttcctttct ataacagaga aacaatagtt aagtatggta atttagtaga ggataacagt
     1681 gacttattta ctaaaaaact tctatcagtt gttcctatg
//
LOCUS       DAVKMU010000116         1555 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKMU010000116 DAVKMU010000000
VERSION     DAVKMU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.116"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(525..1517)
                     /locus_tag="VW343_002118"
     CDS             complement(525..1517)
                     /locus_tag="VW343_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9756580.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          532 a          265 c          193 g          565 t
ORIGIN      
        1 atagtggatt tacccactac aaatattata gaacccaaaa atgctctgaa acactgattt
       61 caaggcattt tttgttagtt taaaattact cccattcttc ttttccaaac atacaatata
      121 tccaaaacca ttcaaaatac tagattctat tttttataat atcactaaat ccacctaatt
      181 ataggacgtt ttcagatttt tagtcccgtc ccagtcccag taccggagaa atattgtttt
      241 aatataatat ctctttttgt cttctaagct cttaaaagca aaagaacaag taaagagtca
      301 agacaaggat aaaaagtcca tattagggca aataaaaagc tttaagacag atgacaaatc
      361 taagtcaaat aagaaagacc atagcaaagg tgcagagaga taaatattgg cggtcttcgg
      421 actgccttta tttttttatc catttttcaa atcaaattta ttcagactat atatgcacat
      481 atacacttaa attcatataa aaacatggct tgtaaaaaat tactttaatc acaataatcg
      541 catttaaaat tgtgatgttt gcaagctaaa ttacggactt cacttggaag ttttcccttg
      601 tatcttttat aatagataga aaatttgctg gcagatgaat atccaacaga ttctgctatc
      661 tcttttatag gtagttcagt gtttaaaaga agagtttcag ctacattcat tctttttctt
      721 tgagtgtact ctgtaatgct ttgacaatat ttttccttaa ataaattttt taatttagta
      781 ccactcattt tagatatttt ttcaagcgtg ccttgattta cattcgttgc aaaatgatca
      841 tctaagaatc ttgctacatc ttcaagtgct ttatcatcat caatttcaat tttatatttt
      901 tttctattta agtatgtgtc aattactata cttatccatt catttgcctt tgctttaaag
      961 aaaaaatcag cggcaggagc gtccatctta caatttaata tttccattgc cactctttct
     1021 aaggcctttg taagtattat ttgattcggt tgaagcaagg ttgaataaaa agattctgga
     1081 ttaatgttaa tagatgctaa atgtttttct attagctctt ttttaaaacc aatggaaaca
     1141 gcaagataac aacaattctc gtgtaataaa aaaacaaaat catcttttat attatcaaaa
     1201 tcaaaagtac ataaagagtt tgcggtaata gtttgatacg gattaaactt ttctccgttt
     1261 gcactgacaa tgtaacttga ataaattgaa acatagtctg acatactata agtgctattt
     1321 tgaactactt cctctttgat ataaaaatca tgtatatcga taatgaagct gcctccttca
     1381 taaaaccaat ataggccttc tgcataagtt gaattctctt ttttccaaca aaatgtatga
     1441 cctgcacttg aatacttctc attgttttta cattcatcta ctttcatata atcttttaca
     1501 aaatcataat atgacataac acactatccc ttttagacaa tattccaatt agcct
//
LOCUS       DAVKMU010000117         1538 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKMU010000117 DAVKMU010000000
VERSION     DAVKMU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.117"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            20..1366
                     /gene="gdhA"
                     /locus_tag="VW343_002119"
     CDS             20..1366
                     /gene="gdhA"
                     /locus_tag="VW343_002119"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9756581.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
BASE COUNT          466 a          308 c          322 g          442 t
ORIGIN      
        1 acaataaaag gagaacgata tgacatctgc taaagaatat atccaaagcg tgtttgaaac
       61 tgtaaaagct cgtaacgggc acgaggctga attcctccaa gctgttgaag aatttttcaa
      121 cactttggaa cctgtatttg aaaaacaccc tgagtatatc gaagaaaata tcttggcacg
      181 tattactgag cctgagcgcg tgatttcttt ccgtgttcct tgggttgacc gtgatggaaa
      241 agttcaagta aaccgcggct accgtgttca attcaactca gctgttggac catacaaagg
      301 tggacttcgt ttccacccaa ctgtaaacca agggatcttg aaattcctcg gatttgaaca
      361 aatctttaaa aatgtcttga ctggacttcc tatcggtgga ggtaaaggtg gatcagactt
      421 cgatcctaaa ggtaaaacag atgctgaagt gatgcgcttc tgccaaagct tcatgactga
      481 attgcaaaaa cacatcggac catcacttga cgtacctgct ggtgatatcg gtgttggtgg
      541 acgtgaaatt ggttaccttt acggtcaata caaacgtctt aaccaatttg atgctggtgt
      601 cttgactggt aaacctcttg gatttggtgg tagcttgatt cgtccagaag caactggtta
      661 cggtttggtt tactatactg aagaaatgct caaagctaac ggtaacagct ttgctggtaa
      721 gaaagtcgtt atttcaggtt ctggtaacgt tgctcaatac gctcttcaaa aagcaaccga
      781 acttggtgca acagttatct ctgtttctga ctcaaacggc tatgttatcg atgaaaacgg
      841 tatcgacttc gatcttttgg ttgatgttaa agaaaaacgt cgcgctcgtt tgactgagta
      901 tgcagctgag aaagcaactg ctacttatca tgaaggttct gtatggactt acgctggaaa
      961 ctatgacatt gcgcttccat gtgctactca aaacgaaatc aacggtgaag cagctaaacg
     1021 tttggttgct caaggcgtta tctgtgtatc tgaaggtgcc aacatgccaa gcaaccttga
     1081 tgccatcaaa gtttacaaag aaaatggtat cttctacgga cctgccaaag ctgccaacgc
     1141 tggtggtgta gccgtttcag cccttgaaat gagccaaaac agccttcgcc tctcatggac
     1201 tcgtgaagaa gttgatggac gtctcaaaga catcatgacc aacatcttta acacagctaa
     1261 aacaacttca gaaacatatg gtcttgataa agactacctt gcaggagcta acattgctgc
     1321 ctttgaaaat gtagcaaacg ctatgattgc acaaggcgtg gtataaaaaa aaattacgga
     1381 aactgactac ccagacgggt agccagtttt ttcaattcct aaaatttaag attctaatac
     1441 cctttatgca aaaatataga attctacctt tttctttata taggctaatg tttttaatat
     1501 atttattgtc tatttttgaa aagacaataa aactattc
//
LOCUS       DAVKMU010000118         1448 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKMU010000118 DAVKMU010000000
VERSION     DAVKMU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.118"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..666
                     /locus_tag="VW343_002120"
                     /pseudo
     CDS             1..666
                     /locus_tag="VW343_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(701..1448)
                     /locus_tag="VW343_002121"
                     /pseudo
     CDS             complement(701..1448)
                     /locus_tag="VW343_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          451 a          244 c          292 g          461 t
ORIGIN      
        1 atagagatag acttaaaaac tatcttactg acaatccaga cgcttatttg actgaaatag
       61 cttctgaatt tggctgtcat ccaactacca tccactatgc gctcaaagct atgggctaca
      121 ctcgaaaaaa agaaccacac ctactatgaa caagacccag aaaaagtagc cttatttctt
      181 aaaaatttta atagtttaaa gcacctagca cctgtttata ttgatgaaac aggattcgat
      241 acttattttt atcgagaata tggtcgctca ttaaaaggtc agttaataag aggtaaagta
      301 tctggaagaa gatatcagag gatttctttg gttgcaggtc taacaaatgg tgagttaatc
      361 gctccaatga cttacgaaga gatggtgacg agcgactttt ttgaagcttg gtttcagaag
      421 tttctcttac caacattaac cacaccatcg gttattatta tggataatgc aagattccat
      481 agaatgggta agttagaact tttatgcgag gagtttgggc ataaactttt acctcttcct
      541 ccctactcac ctgagtacaa tcctattgag aaaacatggg cttatatcaa aaagaacctc
      601 aaaaaggtat taccaagttg caataccttt tacgaggctc ttttctcttg ttcttgtttc
      661 aattgactat attagaggcg agacattttt cggttctttg tcaactgtag tgggttgaag
      721 aaagcgaaga tctagaaagg acaaatttcg tcctttcttt tttgaagttt tcaaagttcc
      781 taaaaccaaa ggcattgtgc ttgataagtt tgatgagatt attggtggct tccagtttgg
      841 cgttggaata aggtaattga agggcgttga cgattttctc tttatctttg aggaaggttt
      901 taaacagagt ctgaaaaaga ggtggaaaag caagagctga tagagattat agctgataga
      961 gattatagtg gtgtttcaag tcttcgaata gctcaaaagt ttatctagaa tttctttatt
     1021 agtcaagtgc atacgaaaag tagggcgata aaatcgttta tcactcagtt tctgactatc
     1081 ttgttgaatg agctccagta gcgcttgata gccttgtatt catgggattt cggatgatgg
     1141 cttgtgttct gctctcaaga acagttatga tattgagctt atcaaagtcc tgagcaataa
     1201 agctcatctc tatctcccga ttgaaacagt cactccccag actgtttcaa cgtcctagga
     1261 cataatctca ggaagacgcg aaaaatcatg ctcaaagtga aaatcattga tcttgcgaat
     1321 gacagttgaa gttgaaatag acaactgatg atcaatgtcg gtcatagaaa tcttttcaat
     1381 taacttttga gcaatctttt ggttgataat acgagggatt tggtgattct tcttgacgat
     1441 agaagttt
//
LOCUS       DAVKMU010000119         1389 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKMU010000119 DAVKMU010000000
VERSION     DAVKMU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.119"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            50..>1389
                     /locus_tag="VW343_002122"
     CDS             50..>1389
                     /locus_tag="VW343_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9756582.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIEIKIDFQEEI
                     QENPDLVEGIVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALSKAVIT"
BASE COUNT          522 a          215 c          270 g          382 t
ORIGIN      
        1 ataataaagg tctttgtaac atatataaat tttatttcaa ggaggaataa tggaaaagta
       61 ttttggtgaa aaacaagagc gtttttcatt tagaaaatta tcagtaggac ttgtatctgc
      121 aacgatttca agtttatttt ttatgtctgt attagctagt tcatctgtgg atgctcaaga
      181 aactgcggga gttcactata aatatgtggc agattcagag ctatcatcag aagaaaagaa
      241 gcagcttgtc tatgatattc cgacatacgt ggagaatgat gatgaaactt attatcttgt
      301 ttataagtta aattctcaaa atcaactggc ggaattacca aatactggaa gcaagaatga
      361 gaggcaagcc ctagttgctg gtgctagctt agctgctctg ggaattttaa tttttgctgt
      421 ttccaagaaa aaggttaaga ataaaacggt attacattta gtattggttg cgggaatagg
      481 aaatggtgtc ttagtttcag tccatgcttt agaaaatcat cttttgctaa attacaatac
      541 ggactatgaa ttgacctctg gagaaaaatt acctcttcct aaagagattt caggttacac
      601 ttatattgga tatatcaaag agggaaaaac gacttctgat tttgaagtaa gtaatcaaga
      661 aaaatcagca gccactccta caaaacaaca aaaggtggat tataatgtta caccaaattt
      721 tgtagaccat ccatcaacag tacaagctat tcaggaacaa acacctgttt cttcaactaa
      781 gccgacagaa gttcaagtag ttgaaaaacc tttctctact gaattaatca atccaagaaa
      841 agaagagaaa caatcttcag attctcaaga acaattagcc gaacataaga atctagaaac
      901 gaagaaagag gagaagattt ctccaaaaga aaagactggg gtaaatacat taaatccaca
      961 ggatgaagtt ttatcaggtc aattgaacaa acctgaactc ttatatcgtg aggaaactat
     1021 agagataaaa atagattttc aagaagaaat tcaagaaaat cctgatttag ttgaaggaat
     1081 tgtaagagta aaacaagaag gtaaattagg taagaaagtt gaaatcgtca gaatattctc
     1141 tgtaaacaag gaagaagttt cgcgagaaat tgtttcaact tcaacgactg cgcctattcc
     1201 aagaatagtc gaaaaaggta ctaaaaaaac tcaagttata aaggaacaac ctgagactgg
     1261 tgtagaacat aaggacgtac agtctggagc tattgttgaa cccgcaattc agcctgagtt
     1321 gcccgaagct gtagtaagtg acaaaggcgt accagaagtt caaccagcgt tgtccaaagc
     1381 agttataac
//
LOCUS       DAVKMU010000120         1351 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKMU010000120 DAVKMU010000000
VERSION     DAVKMU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.120"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            199..539
                     /gene="tnpB"
                     /locus_tag="VW343_002123"
                     /pseudo
     CDS             199..539
                     /gene="tnpB"
                     /locus_tag="VW343_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(572..1351)
                     /locus_tag="VW343_002124"
                     /pseudo
     CDS             complement(572..1351)
                     /locus_tag="VW343_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          410 a          279 c          246 g          416 t
ORIGIN      
        1 ggaaggcagt tcattctaca cccactatta tgcgttttta tcattttgaa atctactgct
       61 aatcaatgaa gaagcaaaga tcaaactagg cagctagtta caagttacgt aagcgcgtca
      121 taacaaggta tctatcctaa ttccccaact tccagtatac tagataaaaa ataattatcg
      181 gaggaaaggt attgtactat gataattcaa ctaagtgatt taggtcaagt tcaccttgtt
      241 tgtagcaaga tagatatgag gcagggaata gactcattag cctatgtagt taaaacccac
      301 tttgaattgg atcctttctc cggtcaagtc tttctctttt gtggtggacg taaagaccgc
      361 tttaaagccc tttactggga tggtcaagga ttttggctac tatataaacg ctttgagaac
      421 ggcagactga cttggcccaa tacagaaaag gatgtcaaag ctctcacacc tgaacaagta
      481 gactggctta tgacgggctt ttctatcact ccaaaaataa atccatcaga aagtcgtgat
      541 ttctattgaa atgaggactt tctttttata ctcatctgct ttcaaaaagc attctattcc
      601 atctccgatt aacgatggac tttatcacct ccttctccag tccttgtata acatcttgga
      661 gttgattcat gacatcttcc aaagttcgaa aggctttatt cttaaatcca cgtttacgaa
      721 tctctttcaa caattgttca atggggttca tctctggtgt gtatggagga ataaatgcaa
      781 agccaatatt agtcggaatc tttaaggtac ttgatttatg ccatacagca ttgtccataa
      841 cgagtaaaag ataatcatct ggataagctt gtgaaagctc ttctaaaaag gcgttcatcc
      901 actcagtatt acatccacca gctattaaga aaaatgattc gcctgtatag gcatcaacag
      961 ctccataaca atagcgaaat tctcgtatat agtgactatg gacatgtaga cctactccta
     1021 ttggagacca acaagatccc agtttactga ttctaccgaa accagcctca tcttggtaca
     1081 tcaagcaaac cttatgaaaa cgtctactgg ttttaaagcg ctttcttttc ttcttgaatt
     1141 gagattttat ttttagacgc gacaatggtt tgagcgtctg ctttcttagg atgttctgga
     1201 cgtggcataa tatttcgcca accatggcgc ttcaacagtt gatagaaggc atcacgtgtg
     1261 taggaacgac ctaactcctt tttataagcc tgaaataagg catcaattgt aacaaattct
     1321 cctgcctctg cagccttcaa atgacgggca a
//
LOCUS       DAVKMU010000121         1309 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKMU010000121 DAVKMU010000000
VERSION     DAVKMU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.121"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..>1309)
                     /gene="lanM"
                     /locus_tag="VW343_002125"
     CDS             complement(<1..>1309)
                     /gene="lanM"
                     /locus_tag="VW343_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEU9756583.1"
                     /translation="IYPWIVKPLVLEINSLREKGLLEGESEQQRYKYFITLFDKEENI
                     LNFYNKYPVLLRQISESCLRFYTYFIEILSNLENDFSVLEEELGLRGKLNDIKFGKGD
                     THSQGKTVLILFFDDAKIVYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDH
                     SYEEFIDYLPLEQKKNLPEYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFET
                     MLQQPLFDDKTGQSLLDTLFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNG
                     QVLINLNTDKVKFDIGKIDFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNK
                     TEEFKMLLKANLYGLKTRVLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKF
                     SNQGIVASECEQMRLLDIPIFYTDTNINEIYDDFGNHFISLHQF"
BASE COUNT          447 a          246 c          150 g          466 t
ORIGIN      
        1 aaaattgatg tagggaaata aaatgatttc caaaatcatc gtagatttca ttgatatttg
       61 tatctgtata aaaaatcgga atatctaata acctcatttg ttcacattcg cttgctacaa
      121 taccttgatt agaaaactta ttgctccaga gattttccaa tgctttttct ctatctaaca
      181 tttcttcata aaaatcagga tgatataaaa aagatagtac tgaagcatag ctatttgtgt
      241 ctctaaaaag tacccttgtc tttaaaccat acaagtttgc ttttaatagc attttaaatt
      301 cttctgtttt atttaactct tcaaatatca gataaaaatc cctaaaacct tttttgaaat
      361 cttttatata cttatcaaat tctatatcac catcccgaac aggcaggttt ttcccacctt
      421 caaaatcaat tttcccaata tcaaacttta ccttatcagt atttaaatta attaaaactt
      481 gaccattaaa agcatccttc ttaaaatttc ctgtaagagc actcatctca acatcaagac
      541 catcctccct ttttactccc tctgttggaa gaagcaaagt tcgagtcacc ctgtgaaaca
      601 gggtatccag caaactctga ccagttttat catcaaatag aggttgctgt aacattgttt
      661 caaagtctat tattacaggc atatctccat aggaaattaa attttcaaaa tgtatatcac
      721 tcccattaaa taaatatata aatgctaaaa gcacaccaaa attataataa tattcaggta
      781 aatttttctt ttgctctaga ggtagataat caataaattc ttcatagctg tgatccgaat
      841 aagcaatagt tcgaggtatt cttatcctaa ttttttcatc aacctttcgg atatactcag
      901 caatagtatt tagtgagtta ttgattatta aatttttagg cttgtaaaca attttcgcgt
      961 catcaaagaa gagtatcaaa acagtttttc cttggctgtg tgtatcaccc tttccaaatt
     1021 ttatatcatt taatttcccc cttagcccta attcttcttc tagcacacta aaatcatttt
     1081 ctaaatttga taaaatttct ataaaataag tatagaaccg aagacaagac tccgatattt
     1141 gcctcagtaa aacgggatat ttgttataaa aatttaatat attctcttcc ttgtcaaaca
     1201 atgttataaa atatttgtac cgttgctgct ccgattcccc ttcaagtaaa cctttttcac
     1261 gcaatgaatt tatctctaaa accaaaggtt taacaatcca aggatagat
//
LOCUS       DAVKMU010000122         1303 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKMU010000122 DAVKMU010000000
VERSION     DAVKMU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.122"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..518)
                     /locus_tag="VW343_002126"
                     /pseudo
     CDS             complement(1..518)
                     /locus_tag="VW343_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(594..821)
                     /locus_tag="VW343_002127"
     CDS             complement(594..821)
                     /locus_tag="VW343_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9756584.1"
                     /translation="MRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSGKVENQAIT
                     LYHHDQSRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            849..1034
                     /locus_tag="VW343_002128"
                     /pseudo
     CDS             849..1034
                     /locus_tag="VW343_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1129..1303
                     /locus_tag="VW343_002129"
                     /pseudo
     CDS             1129..1303
                     /locus_tag="VW343_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          398 a          287 c          237 g          381 t
ORIGIN      
        1 taataatagc tcttgcttta gctccttcaa aactttgaga aaataaccac cctcgctttt
       61 ttaaactgag ctcaggctaa aagagtccac tggactcttt tactccgtcc cataaccaat
      121 gatttaatgg cgcgttcagc tagattattg gaaaggacca gatgtccgtc tttcaaaata
      181 gtcttaaagg tttcttcata cttgaggctg tattcaattg cccttcctag ttttgaacct
      241 gctaaaactg actgacggcg gcaccaagca aagaagtctt ccattagggg ctggagatgt
      301 tcttgacgtt tctgtagtcg ttcatcagct ggcaaagcct cccagtctct ttccaaggaa
      361 aataactgat cacaataagc taaaccttta gctcctaagg atgatttatc tgcttgcttg
      421 ggggtcgctt caaaaaattt ccttctcaca tgcgcccaac atccaacaag ttcagcttct
      481 tccagttgac gataagcttc tagcagttcg cagtttacca agcttagcgt cgccttgctc
      541 ctaaaccttg gactgctatc gcataggcag aactagagga ctaaagtcct aagttactgc
      601 cgcaacatat cacaatgaac atagccagaa taatctccta ggaattcttg tactaccgaa
      661 ccactccgac tctgatcatg atggtacagc gtgattgctt gattctcaac tttcccagac
      721 aaaaaggtcc aatagtaggt cagatggcta tgactctcta gaacccgata agaagtttca
      781 tccgcatgaa gtaagggctg agtcaatagt ctctctcgca agaggttata aaggggcttt
      841 aggaatagga gctttcacaa gcttatccag atgattatct tttactcgtt atggacaatg
      901 ctatatggca taaatcaagt accttaaaga ttccgactaa tattggtttt gcatttattc
      961 ctccatacac accagagatg aaccccattg aacaagtgtg gaaagagatt cgtaaacgtg
     1021 gatttatagt aaaatgaaat aataacagga caaatcattc aggacagtca aatcgatttc
     1081 taacaatgtt ttagaagcag aggtgtacta ttctagtttc aatctactat agataaactt
     1141 ttgagctatt cagaagactt gaaacaccac tataatctct atcaactctt gctttttcac
     1201 tttcagaata aggagcctga caaatttttc ggactcattg aggacaatct gaatcaggtt
     1261 catcctctaa aaccttcctc aaagataaag agaagattgt caa
//
LOCUS       DAVKMU010000123         1301 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKMU010000123 DAVKMU010000000
VERSION     DAVKMU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.123"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..213)
                     /locus_tag="VW343_002130"
     CDS             complement(<1..213)
                     /locus_tag="VW343_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9756585.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     GKEAFRHGHGNRNRKPKHAIPDEIKER"
     gene            377..529
                     /gene="rpmG"
                     /locus_tag="VW343_002131"
     CDS             377..529
                     /gene="rpmG"
                     /locus_tag="VW343_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9756586.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            539..715
                     /gene="secE"
                     /locus_tag="VW343_002132"
     CDS             539..715
                     /gene="secE"
                     /locus_tag="VW343_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9756587.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            770..>1301
                     /gene="nusG"
                     /locus_tag="VW343_002133"
     CDS             770..>1301
                     /gene="nusG"
                     /locus_tag="VW343_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9756588.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT          410 a          204 c          258 g          429 t
ORIGIN      
        1 acgttctttg atttcatcag ggattgcatg ttttggtttt cgatttctgt ttccgtgtcc
       61 gtgtctgaag gcttcttttc ctttctgttg ataggctagt aacagacgat tgatttgtct
      121 ttcagaaaga ttgagttcga cacaggcccg tttctttgtt ttctttcctt gggctatagc
      181 ttttatcaca agatattttt tcgtttcatt catattcagt tggatccttt tcatatgact
      241 attctaccaa atgggacatt ttcacgttcg atttactaaa gacattatca cattcgaatc
      301 acaatagtca atctatttcg ctaagatgct tgtcaaagcc tagctttctt gttataatgg
      361 ataagatgga ggcgttatgg cactaaaaaa agcaagccta gcttgtgcgg tttgtggttc
      421 gagaaactat tcaatcaaga tcagcggaaa ccccaagcct acacgactag aagtaaataa
      481 attttgtaag cattgtggca agtacactac acacagagaa acgagatagg agagagcgat
      541 gcgttttatt ggagatattt ttagacttct taaagacaca acgtggccaa ctcgcaagga
      601 aagctggaga gattttcgtt ctatcatgga atacacagct ttctttgtag taattattta
      661 catttttgac cagttgattg tttcaggttt gattcgattt attaacattt tttagaagat
      721 tagtggagtt aattacacta gaaatcttct atttatgaaa ggaaatatca tggatagttt
      781 tgataaaggg tggtttgttt tacaaactta ttctggttat gaaaataagg tgaaagaaaa
      841 tctattacaa cgtgcacaaa cctacaatat gttggataat attctacgcg ttgaaattcc
      901 aacacaaaca gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt
      961 tccaggttat gttcttgtag aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa
     1021 cacaccaaat gttacaggat ttgtcggatc acacgggaac agatcaaaac caactccatt
     1081 attggaacaa gaaattcgtg acattttggt atctatggga caaactgttc aagaatttga
     1141 tttcgatgtt gagattggtc aaactgtacg tattattgat ggtgcttttg cagactacac
     1201 tggtaagatt acagaaattg ataataacaa agtgaaaatg attatctcta tgtttggtaa
     1261 tgacacagtt gcagaagtaa acctaaacca aatcgcagaa t
//
LOCUS       DAVKMU010000124         1243 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKMU010000124 DAVKMU010000000
VERSION     DAVKMU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.124"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            146..1156
                     /locus_tag="VW343_002134"
     CDS             146..1156
                     /locus_tag="VW343_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin formation protein"
                     /protein_id="HEU9756589.1"
                     /translation="MVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFM
                     YYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLA
                     CLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLG
                     DTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIH
                     SLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELES
                     LLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLL
                     EI"
BASE COUNT          445 a          153 c          220 g          425 t
ORIGIN      
        1 caacttgata tcattgaaat ttattttcca agctttgatc agcgaaaaaa aggatataaa
       61 ttaaacttat ctaatagaaa aaaaactccc aaatgaaagt ttagttacta ttctgcataa
      121 tgaagtagaa aatattgcag actctttggt tattaataat atttatgacg aatatccaat
      181 ttggagtata cctgttaatt ttaaaggttc taaatggtgt cttgaacaaa cgggtaatga
      241 tatgtatagg ggtagagctg gaatattact gtttatgtac tatgtaaaat tattgaaaga
      301 taaatcatca tatactcaac tgtacaataa aatgttatct gcaattccgc catctgtttc
      361 tttatctaag accacatttg gtttaactgg ggatatagga tatttcattg ttctatccat
      421 tatagaggat tacgatacaa atactctcgc atgcttaaat tatataaaga gtgttttaac
      481 tgagcttgaa aagactgatt ttgcaagtgt ttcaaacaag tctgactata ttaatggtct
      541 tctaccttta attaatacac tggtaagata ttataggaga ggaatagaga aggaacgtgt
      601 tctaaggcta gtattaagtt taggtgatac gctatataat gatgtaagcg agagagataa
      661 tagtaatttt ggatatagtt ttggccatgg actatcaggt gtaattttta cattaaaaaa
      721 tatttataaa gtgacaagac ttaaaaaata taaagaatta attttacaac tagttcctag
      781 ggagaaaatc aaaattcatt ctttgaagtg gtgtagtgga gaatttggga cattagttaa
      841 tcatccccaa agtgttccag agggaattgt agctagttta gataatgata cattttgcca
      901 tggaagtatg gccataataa atttttacat cgactacatg aaagaaatgg atactagtat
      961 ggaattggag tctttattgt gtagtgttgt aatgaataaa tcaaaagaag gagagtatcg
     1021 tatcgtccaa acaagcaatt tcccagattt ttctttgtat acaggtgtga ctggtattgc
     1081 ttatactttt ttaaggtata tcgcactgaa tcagaatgac aaaaagatag ttatcccctc
     1141 attacttgaa atctagctta atttatagtt ttgtttctaa aatagaggtg tccactcatt
     1201 aagcaatata aaaataccaa gattgactag tttaatatgt aat
//
LOCUS       DAVKMU010000125         1135 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKMU010000125 DAVKMU010000000
VERSION     DAVKMU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.125"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            313..>1135
                     /locus_tag="VW343_002135"
     CDS             313..>1135
                     /locus_tag="VW343_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9756590.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFATKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVD"
BASE COUNT          333 a          198 c          264 g          340 t
ORIGIN      
        1 ataaataaaa aagcgatatt ctagactgta tcaatccttt tccattatag gagcaacaga
       61 gtgggagtag tcatctaagg actaatttat atatttttac gagtcagtat cttgggatac
      121 tggtttttac ttttttagac tttttgacta cttgttaaaa ctggggtaat tttcgactgt
      181 ttaatagtta ttatgcaaag tctaaaagat tagaattgtc aaaacaatcc gtctaggctt
      241 gattttatcc tttatttact ataaaatgag aaggaaaaat gtcaaacttt tatattgcaa
      301 ataggagaaa tcatgacaaa aacattaaaa cgtcctgagg ttttatcacc tgcagggact
      361 ttagagaagc taaaggtagc tgttcagtat ggagcagatg ctgtctttat cggtggtcag
      421 gcctatggtc ttcgtagccg tgcgggaaac tttactttcg aacagatgga agaaggcgtg
      481 cagtttgcga ccaagtatgg tgccaaggtc tatgtagcgg ctaatatggt tatgcacgaa
      541 ggaaatgaag ctggtgctgg tgagtggttc cgtaaactgc gtgatatcgg gattgcagca
      601 gttatcgtat ctgacccagc cttgattatg attgcagcga ctgaagcacc aggccttgaa
      661 atccaccttt ctacccaagc cagtgccact aactatgaaa cccttgagtt ctggaaagag
      721 ctaggcttga ctcgtgtcgt tttagcgcgt gaggtttcaa tggaagaatt agctgaaatc
      781 cgcaaacgta cagatgttga aattgaagcc tttgtccatg gagctatgtg tatttcatac
      841 tctggacgtt gtactctttc aaaccacatg agtatgcgtg atgccaaccg tggtggatgt
      901 tctcagtcat gccgttggaa atacgacctt tacgatatgc catttgggaa agaacgtaag
      961 agtttgcagg gtgagattcc agaagaattt tcaatgtcag ccgttgacat gtctatgatt
     1021 gaccatattc cagatatgat tgaaaatggt gtggacagtc taaaaatcga aggacgtatg
     1081 aagtctattc actacgtatc aacagtaacc aactgctaca aggcggctgt ggatg
//
LOCUS       DAVKMU010000126          936 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKMU010000126 DAVKMU010000000
VERSION     DAVKMU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.126"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..840
                     /locus_tag="VW343_002136"
                     /pseudo
     CDS             1..840
                     /locus_tag="VW343_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            833..>936
                     /locus_tag="VW343_002137"
     CDS             833..>936
                     /locus_tag="VW343_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9756591.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQS"
BASE COUNT          345 a           84 c          197 g          310 t
ORIGIN      
        1 gttaaaaaaa cttgacaaga atggatttgg aaaggttaag acagtgataa catgccatgg
       61 atgggttgaa tataacttaa aaaagaaatt cttgacttat tttgattttt ggacatattc
      121 tatgggagat gcttttattt gtgtatctga aactatgaaa aaagattgga gagtataata
      181 aaaaataaga agataattgc aataaataac ggaattaatg tatcaaatag cgatttagat
      241 gtagtaggtg ttcaagactt taaaaaagaa ttttgtattc caaataacaa aaaaatcatt
      301 tgttatattg gaaggttgga tccagaaaaa aggccggata gattccttga atttgcagaa
      361 aaattatttt tagtcagaga agatgtgatt tttattatgg ctggaaatgg tagtatgtgg
      421 gctgctttga aagaaaaaat ttgccatttg aaatgtagag ataatttcag gttacttggt
      481 gaaatctatc cagctactat agtttaccaa atatcggatt tattgtatat tccttctgat
      541 acagaaggaa tacctatgtg tgttttagag tcaatgtcac aaggaactcc ggttttagct
      601 agtaatgttg gcgggttaag tgaaattatt gaacataggg ttgatggatt tttatttgag
      661 aaggaagatg ttgagggagt gtgtgcttgt gctaattttt tactcaatga ttctgagtat
      721 ttgaaatata taggtgagaa tagtaaatca aaaataagaa aacatttttc tgtgcaaaaa
      781 atgtttgtag aaaccatgag agtatatgat gaattattag agaagagtag tcatggatag
      841 gaatatagat caagaattag taagtattat aataccaaca cataataggt atgaatctct
      901 gattagagct gtgaaaagtt gtttacatca aagtta
//
LOCUS       DAVKMU010000127          923 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKMU010000127 DAVKMU010000000
VERSION     DAVKMU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.127"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            239..>923
                     /locus_tag="VW343_002138"
     CDS             239..>923
                     /locus_tag="VW343_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEU9756592.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWY"
BASE COUNT          349 a          106 c          169 g          299 t
ORIGIN      
        1 agcttttcat tgagataata atataataaa gaaaagttct attctaattg ctaaacggct
       61 tgaggtatat gctactgtta tagaaatgat tttaaactaa aatcaaaatt actccaaaaa
      121 tagtattgat tttggtttaa tacaaaacat atgtaataat gtgagtcaac aaaagtctgt
      181 ttttgtacca tactatcttg aaaaatctac taaattcaaa ttgagaggat gaattaaaat
      241 gaataaaata tttatatatg caggagtgcg aaatcataac tctaagactt tagagtatac
      301 taagagactt tcaagtatca tttctagtag aaataatgtt gatatatctt tcaggacacc
      361 tttcaattca gagctagaaa tatctaattc tgattcagaa gagttgttta agaaaggaat
      421 tgataggcaa agtaatgctg atgatggagg tgttataaaa aaagagttat tagagtctga
      481 tattataatt atctcttctc ctgtatattt acaaaacgta tctgtggaca caaaaaactt
      541 tattgaaagg attggggggt ggagccatct ttttcgatta gctggaaagt ttgttgtaac
      601 attagatgtt gctgagagta acggctctga taatgtgtca gaatatctta gagatatatt
      661 ttcatatatg ggaggacaga tattacacca agtttcaatt accaattcac tcaaagatat
      721 tgcagaggct cagttgatgg aggcaactta taaaattgaa gatgtacttg aagggaaaat
      781 taagtacaaa acaacggatt atcaagaaag agcttaccag acattaaaat taattttaga
      841 aaattatgat tccgaacatt ttgagaagat gtattgggag aaaaaacgcc tttttgaagc
      901 taatagttta gaggaatggt att
//
LOCUS       DAVKMU010000128          901 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKMU010000128 DAVKMU010000000
VERSION     DAVKMU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.128"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..568
                     /locus_tag="VW343_002139"
                     /pseudo
     CDS             1..568
                     /locus_tag="VW343_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            692..>901
                     /locus_tag="VW343_002140"
     CDS             692..>901
                     /locus_tag="VW343_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9756593.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCP
                     ECGSQMKKYDFQKPSKIPYLETTGMP"
BASE COUNT          313 a          175 c          155 g          258 t
ORIGIN      
        1 tagacttaaa aactatctta ctgacaatcc agacgcttat ttgactgaaa tagcttctga
       61 atttggctgt catccaacta ccatccacta tgcgctcaaa gctatgggct acactcgaaa
      121 aaaaagaacc acacctacta tgaacaagag ccagaaaaag tagccttatt tcttaagaat
      181 tttaatagtt taaagcacct agcacctgtt tagattgatg aaacaggatt cgatacttat
      241 ttttatcgag aatatggtcg ctcattaaaa ggtcagttaa taagaggcaa agtatctgga
      301 agaagatatc agagaatttc tttggttgca ggtctaacaa atgaatggtg agttaatcgc
      361 tccaatgact tacgaagaga cgatgacgag cgactttttt gaagcttggt ttcagaagtt
      421 tctcttacca acattaacca caccatcagt tattattatg gataatgcaa gattccatag
      481 aatgggtaag ttagaactct tgtgtgaaga gtttgggtat aaacttttac ctcttcctcc
      541 ctactcacct gagtacaatc ctattgaggc tctataatat ttgtagtggg taaatcccct
      601 atggatatta tggagcctat ttttgtgtag aaaaaaagtc ccataagatc tataatgaaa
      661 agcgatcaaa caactcatta gaaagattca tatggaacaa ttacatttta tcacaaaatt
      721 actagacatt aaagacccta atatccagat tttagacatc atcaataagg atacacacaa
      781 ggaaatcatc gccaaactgg actacgacgc cccatcttgc cctgagtgcg gaagtcaaat
      841 gaagaaatat gactttcaaa aaccgtctaa gatcccttac ctcgaaacaa ctggtatgcc
      901 t
//
LOCUS       DAVKMU010000129          876 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKMU010000129 DAVKMU010000000
VERSION     DAVKMU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.129"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            96..551
                     /locus_tag="VW343_002141"
     CDS             96..551
                     /locus_tag="VW343_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756594.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            548..694
                     /locus_tag="VW343_002142"
     CDS             548..694
                     /locus_tag="VW343_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756595.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
BASE COUNT          322 a          129 c          161 g          264 t
ORIGIN      
        1 ttttatgtgc agagttatag tggcttgatg ctgggaaagt acactgtgac tgctaaaaca
       61 ttcctagaag ttgctttgat ttacctaatc tatttatgca aattttatgt tattttacta
      121 taacagttgt tgctaagcca aataatagtg gggaagttca tttagacgta agcattgaag
      181 ataatcaggg aggtagtggg cataatttca gttctgtttc aagtagctca caaacagcta
      241 aatatgaaga aactggttat aataacaatt catcattata tataacgatt gataaaacgt
      301 ctgatgcaac agctcttttg aaattaaagt tgaataatgt tgataatcaa cctgctactg
      361 aagttcctag ttcaggaatt actgtaaaat taaatgctaa agataatgct ggaaactgga
      421 caagtgcttc gaataaaaaa gaaataacag taaaaattgt ttctgctaaa ccgacatatc
      481 cagacaaaat cttagtgaaa aatcctgata atataaaaga tacagaaaaa atgccattat
      541 tgaaaaattg aaagaggcaa ataaaaatca tccagcagga gctccaacct ttgctaaagg
      601 tgaaggagag catgcaaatg atattgtagc aacttattca gatggtacaa cttattatgt
      661 accgttaaat gatgtgacaa aatatgcgag gtagtggctg taccactcac ttattcacct
      721 ccccgtgatt tgtagtagtg ataggctttc tcactattat tataaaacaa aataaagatc
      781 acaacacttt ttcattctgt gttgtgcctt gagtgaaacg aaaggaatga attataaata
      841 tgaaaagtat agtcactagc atagatgagc gcttgc
//
LOCUS       DAVKMU010000130          866 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKMU010000130 DAVKMU010000000
VERSION     DAVKMU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.130"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..265)
                     /locus_tag="VW343_002143"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="VW343_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(500..811)
                     /locus_tag="VW343_002144"
     CDS             complement(500..811)
                     /locus_tag="VW343_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9756596.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCSFCFSRFNPGCQNHKERYF
                     K"
BASE COUNT          275 a          171 c          138 g          282 t
ORIGIN      
        1 catccacagc cgccttgtag cagttggtta ctgttgatac gtagtgaata gacttcatac
       61 gtccttcgat ttttagactg tccacaccat tttcaatcat atctggaatg tggtcaatca
      121 tagacatgtc aacggctgac attgaaaatt cttctggaat ctcaccctgc aaactcttac
      181 gttctttccc aaatggcata tcgtaaaggt cgtatttcca acggcatgac tgagaacatc
      241 caccacggtt ggcatcacgc atactctaga cgcaattcta atcttttaga ctttgcataa
      301 taactgttaa acagttgaaa attaacccag ttttaacaag tagtcaaaat accagataaa
      361 ggaaaaaagt ctttaaaatc ttaaaaacgc atagtatcag gtattgaaca atcttgatat
      421 tatgcgtttt attgtgggaa gatttacttc attttctctt ggaattgagt tttctcctac
      481 ttcaaaacta gatgatttgc tatttaaaat agcgttcctt atgattttgg caacctggat
      541 taaagcgaga aaaacaaaag gagcaactta aactttcttt atattgctta tatgttagta
      601 gttttagaca aacaccacat aaaataggct tatcctgtat caaagataag ggatacggct
      661 caaaagggtg attttctaat gaatcatgac accgataaca agcatagtac tttttacaat
      721 catagcattg taaagcaata atatccaact tgctatggta atgaatgcat cttgtttgct
      781 tatccactaa gaaaccctgt gcctctatca tttccttttc ctttcttaag cacgaactgt
      841 gacgctggtt ccatcttcct tataaa
//
LOCUS       DAVKMU010000131          838 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKMU010000131 DAVKMU010000000
VERSION     DAVKMU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.131"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..792
                     /locus_tag="VW343_002145"
     CDS             <1..792
                     /locus_tag="VW343_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element IS1202 family transposase"
                     /protein_id="HEU9756597.1"
                     /translation="FDKQETLNAYYHVLEQILANHGIPLQMKTDKRTVFTYQASNSKK
                     MEDDTYTQFGYACHQLGILLETTSIPQAKGRVERLNQTLQSRLPIELERNKIHTLEEA
                     NTFLLSYIQTFNEQFGNKTKLSVFEEAPNPSERNLILARLAERVVDSGHHIRFQNRCY
                     IPTEQGKEVYFIRKTKALVLKAFDGDIYLNIADKIYHTKELLDHELYSKNFEQEPEQK
                     KERRKYIPPQTHPWKLTSFKQYLHKNKKDYEEFTSEEIHSPQLQV"
BASE COUNT          299 a          200 c          136 g          203 t
ORIGIN      
        1 tttgacaaac aagagacctt gaatgcctac taccatgtcc tcgaacaaat cttagccaat
       61 cacggcattc cccttcaaat gaaaactgat aagagaacgg tctttaccta tcaagcatcc
      121 aactctaaga aaatggagga cgacacctat acacaatttg gatacgcctg tcaccaacta
      181 gggattctcc ttgagaccac ctctatccct caagctaaag ggagggtcga aagactcaat
      241 cagacactac aatcacgcct gcctatcgaa ctggaacgaa acaagattca tacattggaa
      301 gaagccaata ctttccttct ttcctacatc caaaccttta atgaacagtt tggaaataag
      361 acaaaactct ctgtttttga ggaggctcct aacccctctg aacggaatct cattctagct
      421 agactggcgg agagagtcgt cgatagtgga caccatatcc gattccaaaa ccgttgctat
      481 attcctaccg aacaaggaaa agaagtctat ttcatcagaa agacgaaagc attagttcta
      541 aaagcatttg atggtgacat ctacctcaat atcgctgaca aaatctatca cacaaaggag
      601 ctactagatc atgaactcta ttcgaaaaac tttgaacaag aaccagaaca aaaaaaagaa
      661 agacgcaagt atatccctcc acaaacccat ccgtggaaac tcacatcttt caaacaatac
      721 cttcataaaa acaaaaagga ttatgaagag tttactagtg aggagattca ttctcctcaa
      781 ctacaagtat aatctatttt tcgctaaatg ggaagctttt ccttaaagga agtcactc
//
LOCUS       DAVKMU010000132          834 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKMU010000132 DAVKMU010000000
VERSION     DAVKMU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.132"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            105..584
                     /locus_tag="VW343_002146"
     CDS             105..584
                     /locus_tag="VW343_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9756598.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGELSHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            678..749
                     /locus_tag="VW343_002147"
     tRNA            678..749
                     /locus_tag="VW343_002147"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Glu,seq:ttc)
BASE COUNT          311 a          114 c          194 g          215 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattatcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aagcaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagctc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgtgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ctaggaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa agactgtata atagtaagag
      661 ttgaaaataa caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
      721 taacacgggt tcgaatcccg tacggactat ggtatgttgc ggttggaaca cttgatgaaa
      781 aaaagtttaa aaaaacttaa aaatcttcaa aaaagtgttg acaagcgaaa gcag
//
LOCUS       DAVKMU010000133          826 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKMU010000133 DAVKMU010000000
VERSION     DAVKMU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.133"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..518)
                     /locus_tag="VW343_002148"
                     /pseudo
     CDS             complement(1..518)
                     /locus_tag="VW343_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          241 a          180 c          141 g          264 t
ORIGIN      
        1 taataatagc tcttgcttta gctccttcaa aactttgaga aaataaccac cctcgctttt
       61 ttaaactgag ctcaggctaa aagagtccac tggactcttt tactccgtcc cataaccaat
      121 gatttaatgg cgcgttcagc tagattattg gaaaggacca gatgtccgtc ttttaaaatg
      181 gtcttaaagg tttctttata cttgaggctg tattcaattg cccttcctag ttttgaacct
      241 gctaaaactg actgacggcg gcaccaagca aagaagtctt ccattagggg ttggagatgt
      301 tcttgacgtt tctgtagtcg ttcatcagct ggcaaagcct cccagtctct ttccaaggaa
      361 aataactgat cacaataagc taaaccttta gctcctaagg atgatttatc tgcttgcttg
      421 ggggtcgctt caaaaaactt ccttctcaca tgcgcccaac atccaacaag tgcagcttct
      481 tccagttgac gataagcttc tagctgttcg cagtttacca agcttagcgt cgccttactc
      541 ctaaactttg gactgctatc gcataggcag aactagagga ctaaagtcct aagttactgc
      601 cgcaacatgt cacaatgaac atagccagaa taatctccta ggaattcttg ttctttctgt
      661 aaataatttg caaactatag taactgttaa cttttactta accatttttc gattgtaagt
      721 tcaaaatggt caactactat tatattttca ggaatcgtat caaaatcaaa taataaatta
      781 tctgaatgag tttttataac tactccaaaa tcaagttttt tggagt
//
LOCUS       DAVKMU010000134          806 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKMU010000134 DAVKMU010000000
VERSION     DAVKMU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.134"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>806
                     /gene="phtD"
                     /locus_tag="VW343_002149"
     CDS             <1..>806
                     /gene="phtD"
                     /locus_tag="VW343_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9756599.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPL
                     DRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYV
                     EHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEP
                     THPESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSV"
BASE COUNT          285 a          160 c          180 g          181 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatca ggattcagga
      241 aatactgagg caaaaggagc agaagctatc tacaaccgcg tgaaagcagc taagaaggtg
      301 ccacttgatc gtatgcctta caatcttcaa tatactgtag aagtcaaaaa cggtagttta
      361 atcatacctc attatgacca ttaccataac atcaaatttg agtggtttga cgaaggcctt
      421 tatgaggcac ctaaggggta tactcttgag gatcttttgg cgactgtcaa gtactatgtc
      481 gaacatccaa acgaacgtcc gcattcagat aatggttttg gtaacgctag cgaccatgtt
      541 caaagaaaca aaaatggtca agctgatacc aatcaaacgg aaaaaccaaa cgaggagaaa
      601 cctcagacag aaaaacctga ggaagataag gaacatgatg aagtaagtga gccaactcac
      661 cctgaatctg atgaaaaaga gaatcacgct ggtttaaatc cttcagcaga taatctttat
      721 aaaccaagca ctgatacgga agagacagag gaagaagctg aagatactac agatgaggct
      781 gaaattcctc aagtagagca ttctgt
//
LOCUS       DAVKMU010000135          785 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKMU010000135 DAVKMU010000000
VERSION     DAVKMU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.135"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..>785)
                     /locus_tag="VW343_002150"
     CDS             complement(<1..>785)
                     /locus_tag="VW343_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9756600.1"
                     /translation="LNIVFVTTRTKYCSQILQLLLKYFDKNPNKISEVYSILEANYGL
                     VTEREYIDYTLAENTISELANLDLTKSYNQELIVTILKQFLKIEIERTETHEEKFTFG
                     RYKVPDSEKLKQYHRSILKLLANLYNIGSCETRFYIEKMLYDYRRKILTYSESHRNTI
                     FGDLRNIRKLFFNDIKNLSMIGEKIVYALHKAEVKENLPIVFDDYIISDRQKIYNNLT
                     NPNHAWFYDASEIKLQQIANSYSNVWLKIFNFANQFKHSLFMN"
BASE COUNT          270 a          119 c           90 g          306 t
ORIGIN      
        1 cattcataaa taagctatgt ttgaattgat ttgcaaaatt aaaaatttta agccaaacat
       61 ttgaatagct atttgcaatt tgttgtagtt taatttcaga agcatcataa aaccaagcat
      121 ggttaggatt tgttaaatta ttatatattt tttgtctgtc agaaattata taatcatcaa
      181 aaacaatcgg taaattttct ttcacttcgg ctttatgcaa agcataaact attttttctc
      241 caatcataga taagttttta atgtcattaa aaaataattt cctaatattt ctcaaatctc
      301 caaagatagt attacgatga ctttctgaat aggtcaaaat ttttcttctg taatcatata
      361 gcattttctc aatataaaat ctagtttcac aactccctat attatacaaa ttcgctaaaa
      421 gttttagtat acttctatga tactgtttta atttttcact atccgggact ttatagcgcc
      481 caaaagtaaa tttctcttca tgagtctctg ttctttcgat ttcaatttta agaaactgct
      541 tcaaaattgt cacgattaat tcttggttat aacttttcgt caaatcaaga ttagcaagtt
      601 cagaaatggt attttcagct aaggtataat caatatattc tctttctgta accaacccgt
      661 agtttgcttc aagaatacta taaacctcag aaattttatt aggattttta tcaaagtact
      721 ttaacaataa ttggagaatt tgactacaat attttgtccg tgtagttaca aatacaatat
      781 ttaaa
//
LOCUS       DAVKMU010000136          703 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKMU010000136 DAVKMU010000000
VERSION     DAVKMU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.136"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..661
                     /locus_tag="VW343_002151"
     CDS             <1..661
                     /locus_tag="VW343_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9756601.1"
                     /translation="ALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLKDIS
                     FKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDV
                     NHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASV
                     MCKNSPFVFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFE
                     L"
BASE COUNT          274 a           77 c          133 g          219 t
ORIGIN      
        1 tgctttaagt ttaagagata aagaattaag taaattaaaa gttccttatt taaaagaagg
       61 tggagagtat cagataaaaa atcttagcta caaatttact gatgatgagt gtttaagctt
      121 aaaagatatt tcgttcaagc ttgggaaaat ttatggcata ataggatcca acggacgagg
      181 aaaatcaacg cttttaagat gtttaatagg tcttgagaaa aaatcaaaag aagaaattta
      241 ttttaaggga gagaagctat ctaaaaaaga aagactcaaa aactcttcac ttgttatgca
      301 agatgtaaat catcaattat tcacagatga agtattcaac gagcttagat taggagtaaa
      361 gaattttgat gaagaaaagg cgaaaatcat tttaaaagat ttaggcctgg acgaatttat
      421 tgaaaggcat ccgatgagtt tatcaggagg gcaaaagcaa aggcttgcaa tagcatctgt
      481 tatgtgcaag aattctccat ttgtcttttt tgacgaacct tcaagtggta tggattattc
      541 caatatgata aaaatatctg aactgattaa taagtataaa accatggata aaataatttt
      601 tattgtttcc catgatatag aatttttaaa tgaagtggca gatgaaattt ttgaattgta
      661 aaatagagtc tgttaaacat gtctatagaa ctggtaaatt gtc
//
LOCUS       DAVKMU010000137          690 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKMU010000137 DAVKMU010000000
VERSION     DAVKMU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.137"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..513
                     /locus_tag="VW343_002152"
                     /pseudo
     CDS             1..513
                     /locus_tag="VW343_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            601..690
                     /locus_tag="VW343_002153"
                     /pseudo
     CDS             601..690
                     /locus_tag="VW343_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          252 a          122 c          123 g          193 t
ORIGIN      
        1 taaaccacca agtaaaagga acaaaaccaa gaaaagttga tagagataga cttaaaaact
       61 atcttactga caatccagac gcttatttga ctgaaatagc ttctgaattt ggctgtcatc
      121 caactaccat ccactatgcg ttcaaagcta tgggctacac tcgaaaaaaa gaaccacacc
      181 tactatgaac aagacccaga aaaagtagcc ttatttctta agaattttaa tagattaaag
      241 cacctagcac ctgtttagat tgacgaaaca ggattcgata cttattttta tcgagaatat
      301 ggtcgctcat taaaaggtca gttaagaaga ggcaaagtat ctggaagaag atatcagagg
      361 atttctttgg ttgcaggtct aacaaatggt gagttaatcg ctccaataac ttacgaagag
      421 acgatgacga gcgacttttt tgaagtatgg tttcagaaat ttctcttacc aacattaaac
      481 acaccatcgg ttattatagt aaaacgaaat aagaatagga caaatcgatc aggacagtaa
      541 aatcgaattc taacaatgtt ttagaagtag atgtgtacta ttctagtttc aatctactat
      601 attatggata atgcaagatt ccatagaatg gggaagctag aacttttatg cgaagagttt
      661 gggcataaac ttttacctct tcctccctac
//
LOCUS       DAVKMU010000138          654 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKMU010000138 DAVKMU010000000
VERSION     DAVKMU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.138"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>654
                     /locus_tag="VW343_002154"
     CDS             <1..>654
                     /locus_tag="VW343_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9756602.1"
                     /translation="DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKN
                     SRGKYISFLDDDDEYMPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDF
                     VGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVR
                     EFLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNI"
BASE COUNT          254 a           65 c          121 g          214 t
ORIGIN      
        1 tgataattat agtaatgtaa atttaagaaa taaaattatc catcaatttg gctatacaaa
       61 tcatagaatt aagttaattt taagtaatga agatttaggt gcaactaatg ccagaaacat
      121 aggtataaaa aattctagag gtaagtatat atcattttta gacgatgatg atgaatatat
      181 gccagatcga attttaaagt tgatggcttg ttttaaaaag agtagaatga agaatttagc
      241 tttagtttat agttatggaa taataattta tccaaatggt acacgagaag aggagaagac
      301 cgattttgtt ggaaatccct tgtttgttca aatggttcac aatatagcag gtacgtcatt
      361 ttggttgtgt aaaaaagagg tgctagaatt aattaatggt tttgagaaaa tagattcaca
      421 tcaggacggt gttgttttat taaaactact tgctcaagga taccaaattg atatagtgcg
      481 agaattcttg gtgaattact acgctcacag taaagaaaac ggtatcactg gagtgacaca
      541 aaaaacaatt aatgcagatg aagaatatta taattactgt aggaaatatt ttaatttatt
      601 gagtttcaac gagagaatat tggttacaaa gaaatattat tctttaaaca taaa
//
LOCUS       DAVKMU010000139          467 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKMU010000139 DAVKMU010000000
VERSION     DAVKMU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.139"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
BASE COUNT          167 a           55 c           79 g          166 t
ORIGIN      
        1 gatttaatta ataacgaaaa ttatttattc gaatatgcat gtagaattag agaggaatgg
       61 agaaaaaaat gctttaatca ttcttatcgt tttctatgct cacctataat tacagatgat
      121 tttcttaaca cgaagacatt gagaagtagc caaattgaat ataaatatga gcgatattta
      181 tcgaaaagtt cgataggcga tagagcggtt gatggctttg tttccttcaa tactttaaca
      241 gctaatggta tgtctgctat taaactatgt cttgagatat taaactctat tttcttcaag
      301 aagaagattg atttattata ttcaaccgga tattatgaaa caagattttt attaaataat
      361 cttgctaaat caggtattag ttgctatgag gtaagtaatt gtgaattgga taaagataaa
      421 ttttataatg tattcatgat ggaacccaat cgagccgatt taacatt
//
LOCUS       DAVKMU010000140          452 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKMU010000140 DAVKMU010000000
VERSION     DAVKMU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.140"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..452
                     /locus_tag="VW343_002155"
                     /pseudo
     CDS             1..452
                     /locus_tag="VW343_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          166 a           90 c           73 g          123 t
ORIGIN      
        1 tcttcttcta aaagccatta aactagctcg ttcgacctac tactatcact tgaaacagct
       61 agacaaaaca gataaggacc aagagcttaa agctgaaatt caatccatct ttatcgaaca
      121 caagggaaat tatgcttatc gtcggattta tttagaacta agaaatcgtg gttatctggt
      181 aaatcataaa agagttcaac acttgatgaa gtactcaatt tacaagctaa aacgcgacag
      241 aaacgaaaat attcttctca taaaggagac gttggcaaga aagcagagaa tctcattcaa
      301 ggccaatttg aaggctctaa aacaatggaa cagtgctaca cagatgtgac tgaatttgcc
      361 attccagtaa gtactcaaaa gctttactta tcaccagttt tagatggctt taatagcgaa
      421 attatcgcct ataatctttc aacttcaccc aa
//
LOCUS       DAVKMU010000141          379 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKMU010000141 DAVKMU010000000
VERSION     DAVKMU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.142"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..345)
                     /locus_tag="VW343_002156"
     CDS             complement(<1..345)
                     /locus_tag="VW343_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU9756603.1"
                     /translation="MWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVVSYD
                     DAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIK
                     VPQPVQSGDMVRA"
BASE COUNT          101 a           88 c           77 g          113 t
ORIGIN      
        1 tgcacgaacc atgtctcctg attgaacggg ttgtgggact ttaatagtca atagttccat
       61 tggatttgga gcgcggtcga ttttattgcc tttggcatca tgcaaatctt caatataggt
      121 ttcaaaatga cggaaacctg gaccataaaa ctcaacttgg tccccttcgt taatgacgtt
      181 tcgttgacga atggttgctg tttgtgccgc atcatcataa gaaaccactt cagcgacaaa
      241 cttgtactca ggaattttac ggcgagcacc aaacaactgc tcattttcag atggtatacc
      301 gtagtagaaa cctgttgcca attcacgttg ggcaaccttc cacatctcgt ccaccaagtc
      361 ttgtttgata gcttcaaac
//
LOCUS       DAVKMU010000142          334 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064480
            SAMN33606457-rid17954023.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKMU010000142 DAVKMU010000000
VERSION     DAVKMU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606457
            Sequence Read Archive: SRR23727338
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722380.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722380.1
            Genome Coverage       :: 251x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:09:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,108
            Genes (coding)                    :: 1,970
            CDSs (with protein)               :: 1,970
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 71 of 138
            Pseudo Genes (incomplete)         :: 63 of 138
            Pseudo Genes (internal stop)      :: 38 of 138
            Pseudo Genes (multiple problems)  :: 28 of 138
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606457-rid17954023.denovo.143"
                     /strain="AUSMDU00064480"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>334
                     /locus_tag="VW343_002157"
     CDS             <1..>334
                     /locus_tag="VW343_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEU9756604.1"
                     /translation="ETYKPNVLHFCELLAEEEGIKLSDTTVRKILYKKNILSPKSHRK
                     TKKRVRKQAKLNLNQPLDNPILPTAKDFLEDPKKVHPSRPRKKFAGELIQMDASPHAW
                     FGPETTNL"
BASE COUNT          130 a           76 c           55 g           73 t
ORIGIN      
        1 atgaaacata taaaccaaat gtccttcatt tctgtgaatt actagctgaa gaagagggaa
       61 ttaagctctc tgatacaact gttagaaaaa tactctataa gaaaaacatc ctttctccta
      121 agtctcacag aaagacaaag aagagagtaa gaaaacaagc taaactgaac ctgaatcaac
      181 ccctagacaa tccaatctta ccgacggcta aagacttcct ggaagaccct aaaaaggtac
      241 atcctagtag acccagaaag aaatttgctg gagaactcat tcaaatggat gccagccctc
      301 atgcctggtt tggaccagaa accaccaact taca
//
